LOCUS       DACDXO010000001       146938 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E23605_2017
            SAMN12639989-rid9881463.denovo.001, whole genome shotgun sequence.
ACCESSION   DACDXO010000001 DACDXO010000000
VERSION     DACDXO010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639989
            Sequence Read Archive: SRR10023389
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 146938)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 146938)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741914.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:42:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,972
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 33 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 17 of 73
            Pseudo Genes (multiple problems)  :: 10 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..146938
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639989-rid9881463.denovo.001"
                     /isolate="E23605_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(19..729)
                     /locus_tag="IWM35_000001"
     CDS             complement(19..729)
                     /locus_tag="IWM35_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HAP9917890.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
     gene            complement(726..2198)
                     /locus_tag="IWM35_000002"
     CDS             complement(726..2198)
                     /locus_tag="IWM35_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAP9917891.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            2336..2809
                     /locus_tag="IWM35_000003"
     CDS             2336..2809
                     /locus_tag="IWM35_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP9917892.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            complement(2962..3513)
                     /locus_tag="IWM35_000004"
     CDS             complement(2962..3513)
                     /locus_tag="IWM35_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAP9917893.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            3618..4148
                     /locus_tag="IWM35_000005"
     CDS             3618..4148
                     /locus_tag="IWM35_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HAP9917894.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            complement(4631..5074)
                     /locus_tag="IWM35_000006"
     CDS             complement(4631..5074)
                     /locus_tag="IWM35_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9917895.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(5090..6250)
                     /locus_tag="IWM35_000007"
     CDS             complement(5090..6250)
                     /locus_tag="IWM35_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP9917896.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKKQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            6757..8457
                     /locus_tag="IWM35_000008"
     CDS             6757..8457
                     /locus_tag="IWM35_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9917897.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQKKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
     gene            complement(8559..9392)
                     /locus_tag="IWM35_000009"
     CDS             complement(8559..9392)
                     /locus_tag="IWM35_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HAP9917898.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCQVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQSQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(9696..11861)
                     /locus_tag="IWM35_000010"
     CDS             complement(9696..11861)
                     /locus_tag="IWM35_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9917899.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            12121..14415
                     /locus_tag="IWM35_000011"
     CDS             12121..14415
                     /locus_tag="IWM35_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HAP9917900.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGFPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            14408..15073
                     /gene="pgmB"
                     /locus_tag="IWM35_000012"
     CDS             14408..15073
                     /gene="pgmB"
                     /locus_tag="IWM35_000012"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HAP9917901.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKCH
                     DHG"
     gene            15066..16073
                     /locus_tag="IWM35_000013"
     CDS             15066..16073
                     /locus_tag="IWM35_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAP9917902.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            16095..17114
                     /locus_tag="IWM35_000014"
     CDS             16095..17114
                     /locus_tag="IWM35_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP9917903.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDPVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
     gene            complement(17395..18762)
                     /gene="nhaC"
                     /locus_tag="IWM35_000015"
     CDS             complement(17395..18762)
                     /gene="nhaC"
                     /locus_tag="IWM35_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HAP9917904.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFVFFSILAPVFTILGGFLLNR
                     RRVKN"
     gene            complement(18872..20293)
                     /locus_tag="IWM35_000016"
     CDS             complement(18872..20293)
                     /locus_tag="IWM35_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP9917905.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            complement(20536..22413)
                     /gene="tdc"
                     /locus_tag="IWM35_000017"
     CDS             complement(20536..22413)
                     /gene="tdc"
                     /locus_tag="IWM35_000017"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HAP9917906.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            complement(22704..23960)
                     /locus_tag="IWM35_000018"
     CDS             complement(22704..23960)
                     /locus_tag="IWM35_000018"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HAP9917907.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEVEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     gene            24540..25841
                     /locus_tag="IWM35_000019"
     CDS             24540..25841
                     /locus_tag="IWM35_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HAP9917908.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(25910..26509)
                     /locus_tag="IWM35_000020"
     CDS             complement(25910..26509)
                     /locus_tag="IWM35_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP9917909.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
     gene            26763..27644
                     /locus_tag="IWM35_000021"
     CDS             26763..27644
                     /locus_tag="IWM35_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9917910.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTVVEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            27657..29267
                     /locus_tag="IWM35_000022"
     CDS             27657..29267
                     /locus_tag="IWM35_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetronasin resistance protein"
                     /protein_id="HAP9917911.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAEGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRMGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(29344..30003)
                     /locus_tag="IWM35_000023"
     CDS             complement(29344..30003)
                     /locus_tag="IWM35_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAP9917912.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVAVYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(30286..30951)
                     /locus_tag="IWM35_000024"
     CDS             complement(30286..30951)
                     /locus_tag="IWM35_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9917913.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
     gene            complement(31098..31703)
                     /gene="dhaL"
                     /locus_tag="IWM35_000025"
     CDS             complement(31098..31703)
                     /gene="dhaL"
                     /locus_tag="IWM35_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit L"
                     /protein_id="HAP9917914.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            complement(31731..32714)
                     /gene="dhaK"
                     /locus_tag="IWM35_000026"
     CDS             complement(31731..32714)
                     /gene="dhaK"
                     /locus_tag="IWM35_000026"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HAP9917915.1"
                     /translation="MKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRVVAKNQKAEQ
                     VGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEADSGKGVLLIV
                     KNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAGTVLVHKIVG
                     DAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDEIEYGVGIHG
                     EPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQFVFMNDVLA
                     LLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTISW"
     gene            complement(32716..33111)
                     /gene="dhaM"
                     /locus_tag="IWM35_000027"
     CDS             complement(32716..33111)
                     /gene="dhaM"
                     /locus_tag="IWM35_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HAP9917916.1"
                     /translation="MKKSILLISHSQKVTDGIKEMIEQMQKSEEVQIFSLGGINGEIG
                     SDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEEEQQQRYHLVDAPLVEGA
                     FAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            33265..33585
                     /locus_tag="IWM35_000028"
     CDS             33265..33585
                     /locus_tag="IWM35_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HAP9917917.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            complement(33697..34566)
                     /locus_tag="IWM35_000029"
     CDS             complement(33697..34566)
                     /locus_tag="IWM35_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP9917918.1"
                     /translation="MGKVMKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTI
                     SSSGAENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELE
                     EALEAAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQ
                     QAKEFNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAAS
                     FQRIKDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGV
                     LVAAAYGIIRKKK"
     gene            complement(34662..35936)
                     /locus_tag="IWM35_000030"
     CDS             complement(34662..35936)
                     /locus_tag="IWM35_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003745485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP9917919.1"
                     /translation="MLRYLFVYYVFFLVSYPILGGFVWFIGVWCYVFLYKHKQKEWVD
                     VPLSVEPFITIMVPAHNEEIVIEDTIEYLMTKLNYHNYEVLVTDDGSTDQTPEILARL
                     MKKYANLRVVRIEKNKGKAHAFNIGLAFAKGKLILSNDADTVPEPDALIRYVNYFIRP
                     GARHIAAVTANMDVQNRTKLIAKSQTVEFSSIVGIIKRTQSAVFGGLYAYSGANTMYR
                     KEALIDVGGFRQDRATEDISIAWDHQLNDWLSVFAPEIIFFMEVPVTLKMLYRQRKRW
                     AKGGTEVWLTNFKKVMLHPFKHIGRTIIFIDQTLSIVWSIFFCISVVLFAGLIGHYVY
                     QGNYEQIYITFTFSFVFICFEMVAGFFQLLASLIVDDRRRKLKYLLFAPLYMLLFWIV
                     NAITIVTTFIPAVKTILGYGSGTWKSPERTKK"
     gene            complement(35962..36471)
                     /locus_tag="IWM35_000031"
     CDS             complement(35962..36471)
                     /locus_tag="IWM35_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9917920.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFEYEDEKLKKRQELLEEVYTERFGTKEERETVRFYSVSEEKNLDTTFIADL
                     YKENGVELK"
     gene            complement(36646..38781)
                     /locus_tag="IWM35_000032"
     CDS             complement(36646..38781)
                     /locus_tag="IWM35_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP9917921.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            complement(38864..40519)
                     /locus_tag="IWM35_000033"
     CDS             complement(38864..40519)
                     /locus_tag="IWM35_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAP9917922.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            complement(40521..40835)
                     /locus_tag="IWM35_000034"
     CDS             complement(40521..40835)
                     /locus_tag="IWM35_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAP9917923.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            41005..41265
                     /locus_tag="IWM35_000035"
     CDS             41005..41265
                     /locus_tag="IWM35_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HAP9917924.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            41279..41593
                     /locus_tag="IWM35_000036"
     CDS             41279..41593
                     /locus_tag="IWM35_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur reduction protein DsrE"
                     /protein_id="HAP9917925.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
     gene            complement(42323..42877)
                     /locus_tag="IWM35_000037"
     CDS             complement(42323..42877)
                     /locus_tag="IWM35_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HAP9917926.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(43003..44931)
                     /locus_tag="IWM35_000038"
     CDS             complement(43003..44931)
                     /locus_tag="IWM35_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HAP9917927.1"
                     /translation="MDNEMISQLQFDRIKKEIQARAIGNYSKKRISDMTVSTNLQTVL
                     DRQEETREARLILESSQHVPFMGLPRIDALTEQVKKGLILQPTDLIEYADFLRSSRMI
                     TKFFDKNQYQTPLLFAYSKHLPDLINVEELIDQKIKNNKVSDDASRNLRKVRKQLQLI
                     EKEIQSKLLKFLRHPKNKEMIQEAMIVQKGEYYTIPIKASYKNKIDGTIIDESNKRTT
                     VFIEPTVISKLNEHYQLLKAEEISEEYQVLAALTGAIAENEEVIDLLIETITVLDIIF
                     ARAKFSREINGSTPRINKSEHIIIKQGRHPFLPEHAVPLDVEIGKDYRGLIITGANAG
                     GKTVVLKTVGLLTLMAMFGMQVPAKEGTELAVFDEIFVDVGDHQNLENALSTFSGHMQ
                     NIAAILKKIKRNTLVLLDEIGSGTEPNEGAALAIAIMESMYEQGALIIATTHYGEIKK
                     FAGDHEDFVPAAMAFDREALRPKYQLRVGETGESQALWIAHKMDMSMKLIHQAERYLA
                     EKAYRTQKKEFLPKTAVTNRKENKKERQLFAKGDRIFVNEYQKEALVYEDIGEDTIDV
                     YLDKKIIHVPRQRVRLVRSAEDLYPTGYDLDSLFIDYKTRKRQRDLERGSKKAHKVLV
                     KEMRKRQEERRVNDENSK"
     gene            complement(45237..45953)
                     /locus_tag="IWM35_000039"
     CDS             complement(45237..45953)
                     /locus_tag="IWM35_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HAP9917928.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            complement(45954..47777)
                     /gene="glpO"
                     /locus_tag="IWM35_000040"
     CDS             complement(45954..47777)
                     /gene="glpO"
                     /locus_tag="IWM35_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HAP9917929.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            complement(47791..49287)
                     /gene="glpK"
                     /locus_tag="IWM35_000041"
     CDS             complement(47791..49287)
                     /gene="glpK"
                     /locus_tag="IWM35_000041"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HAP9917930.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            complement(49423..50883)
                     /locus_tag="IWM35_000042"
     CDS             complement(49423..50883)
                     /locus_tag="IWM35_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP9917931.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            complement(50873..52168)
                     /locus_tag="IWM35_000043"
     CDS             complement(50873..52168)
                     /locus_tag="IWM35_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAP9917932.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVEEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            complement(52500..53177)
                     /locus_tag="IWM35_000044"
     CDS             complement(52500..53177)
                     /locus_tag="IWM35_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HAP9917933.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            complement(53207..54298)
                     /locus_tag="IWM35_000045"
     CDS             complement(53207..54298)
                     /locus_tag="IWM35_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HAP9917934.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEEAYKELYLPSPLYNFLKARYQ"
     gene            complement(54399..54833)
                     /locus_tag="IWM35_000046"
     CDS             complement(54399..54833)
                     /locus_tag="IWM35_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP9917935.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            complement(55417..56874)
                     /gene="ascB"
                     /locus_tag="IWM35_000047"
     CDS             complement(55417..56874)
                     /gene="ascB"
                     /locus_tag="IWM35_000047"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAP9917936.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENW
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIKDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(56871..58766)
                     /locus_tag="IWM35_000048"
     CDS             complement(56871..58766)
                     /locus_tag="IWM35_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9917937.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPITLLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSPVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(58824..60599)
                     /locus_tag="IWM35_000049"
     CDS             complement(58824..60599)
                     /locus_tag="IWM35_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP9917938.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARLPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYFVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESWEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(60649..62145)
                     /locus_tag="IWM35_000050"
     CDS             complement(60649..62145)
                     /locus_tag="IWM35_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP9917939.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     VIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRTWKEEDCAPVMLDGDEQCLQANTADYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(62339..63961)
                     /locus_tag="IWM35_000051"
     CDS             complement(62339..63961)
                     /locus_tag="IWM35_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HAP9917940.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESINAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTVPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(64410..65723)
                     /locus_tag="IWM35_000052"
     CDS             complement(64410..65723)
                     /locus_tag="IWM35_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAP9917941.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCVLMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGIAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLFGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFVGFMWILNVK"
     gene            complement(65968..66192)
                     /locus_tag="IWM35_000053"
     CDS             complement(65968..66192)
                     /locus_tag="IWM35_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9917942.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(66212..66580)
                     /locus_tag="IWM35_000054"
     CDS             complement(66212..66580)
                     /locus_tag="IWM35_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAP9917943.1"
                     /translation="MIQKTRVMLYVNDVEMICRFFVEKIGAEISETIELPEEFKSIVL
                     SISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETIYENMEEPGEITDNNGILT
                     FNFSDPEGNYFVIGKAESSD"
     gene            complement(66778..67488)
                     /locus_tag="IWM35_000055"
     CDS             complement(66778..67488)
                     /locus_tag="IWM35_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9917944.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGGESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(67506..68003)
                     /locus_tag="IWM35_000056"
     CDS             complement(67506..68003)
                     /locus_tag="IWM35_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9917945.1"
                     /translation="MTPSDVDLLQQISIETFTDTFGKDNSPEHLADYLTQAYALEKLT
                     EELKDKETDFFLVSIDQEPAGYLKLNTGTSQSEEMADHFLEIERIYVRKKFKHQGIGT
                     FLLDTAIEKAKEKQKTAIWLGVWEHNDVALAFYRKKGFIKYSEHVFYMGDDPQTDYLF
                     IKQLF"
     gene            complement(68040..68492)
                     /locus_tag="IWM35_000057"
     CDS             complement(68040..68492)
                     /locus_tag="IWM35_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003331879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP9917946.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            complement(68627..69232)
                     /locus_tag="IWM35_000058"
     CDS             complement(68627..69232)
                     /locus_tag="IWM35_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HAP9917947.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
     gene            complement(69682..70302)
                     /locus_tag="IWM35_000059"
     CDS             complement(69682..70302)
                     /locus_tag="IWM35_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP9917948.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLCQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            complement(70304..71173)
                     /locus_tag="IWM35_000060"
     CDS             complement(70304..71173)
                     /locus_tag="IWM35_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase family
                     protein"
                     /protein_id="HAP9917949.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANEAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            complement(71196..71603)
                     /locus_tag="IWM35_000061"
     CDS             complement(71196..71603)
                     /locus_tag="IWM35_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HAP9917950.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDKHKLAKLYEQVGLKFSELYVNDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            complement(71622..72911)
                     /locus_tag="IWM35_000062"
     CDS             complement(71622..72911)
                     /locus_tag="IWM35_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018378547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP9917951.1"
                     /translation="MGVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGGFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     VLLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFINATATDVDTVAAALLYAWILSPVLKAF"
     gene            complement(72938..73240)
                     /locus_tag="IWM35_000063"
     CDS             complement(72938..73240)
                     /locus_tag="IWM35_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP9917952.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
     gene            complement(73265..75358)
                     /locus_tag="IWM35_000064"
     CDS             complement(73265..75358)
                     /locus_tag="IWM35_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAP9917953.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKVFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLVEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            complement(75847..76611)
                     /locus_tag="IWM35_000065"
     CDS             complement(75847..76611)
                     /locus_tag="IWM35_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HAP9917954.1"
                     /translation="MKKINYLAIILLVGLCLFALPTQGFAVAGGHGGGTSSGRSSGRG
                     SFGGRSYGGSYFGSRSYGRDSYGYYGPGTGPSSYEQTGFLALAVLSVGGVSAFSIVKN
                     KRQKKRLKAEILSRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFA
                     EHQKVLQKNKADGVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLI
                     SGSKTQRQYFSQIWYFDFSEKEKRWQADFIQPIMLD"
     gene            complement(76722..77591)
                     /gene="mdcB"
                     /locus_tag="IWM35_000066"
     CDS             complement(76722..77591)
                     /gene="mdcB"
                     /locus_tag="IWM35_000066"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase MdcB"
                     /protein_id="HAP9917955.1"
                     /translation="MINDERKKAVTQQLAALVEEALIAEVTLSPKPGLVDALDAGAHS
                     DMDYALFLKSAKTLTPFFEEMAQIAWHHAIDQELRERIAEIGRKAEKAMLIATNGINT
                     HKGAIWAMGLLISVIAQKISMEQPFSLSKILDDVSKLAAFPDTKYRHKKTTHGHRVKQ
                     KYGVNGAYEEAILGYPHIRLAIQAAKELDNDSYVQKQMHMLLVLMSSLDDTCVLHRSD
                     QKTLVEMQKLAHAASQSALPNRPFTALLHFCQENQISPGGSADLLSASLFMLKIEELW
                     TADTYICPMLLND"
     gene            complement(77616..78692)
                     /locus_tag="IWM35_000067"
     CDS             complement(77616..78692)
                     /locus_tag="IWM35_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HAP9917956.1"
                     /translation="MFENKKIKINVSDFQKVSAVTAVMMQTILSFALANSHSATNTAF
                     LGTFYVIVKYTAPMFIFAIVYNMVKTSQHLSYWEFLKEKFFELVVPYLLWTTAYLWLF
                     PAVQQQTPYTDTASFLLKYVTGDGAPHLWYTVMMLQIQLFMPFFVWLGYKVFANKKYV
                     WPVLIGSTILYVAWYLFYQTQVLTGPYHDSWYLLDRFVASFMIYGVAAFVYHEKVYQY
                     LDRVRYLFLPVALVIAFFSVRSLLAHPGDLSFANAPYLNTIQSLYSLVIIFAVFIGAS
                     KMIVNDSPKLPLFKWLSVYAYRTYLANVFVFQVLLLLFKDSWLQLPSGVMILVAYLMT
                     ASCAFALSWLLHIIWVAIKKGFSK"
     gene            complement(78716..79309)
                     /locus_tag="IWM35_000068"
     CDS             complement(78716..79309)
                     /locus_tag="IWM35_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase holo-ACP synthase"
                     /protein_id="HAP9917957.1"
                     /translation="MIQAHDIVLFDKHEIEGFTLPEWVVLTEKMFGTVRRASTDHAEI
                     LPIGLRGPARNQRWSTEISKHAVKQVIHPWDIIEQESYRQEKIMDYPVYQQFHAARNI
                     LSGCKWGIGGSLGFELSTGIPAVKETSDFDLLLYANSPIELPIQVIQSHPAFFEQFDT
                     QVVTRQGGFSLKEYLREPEKKILLKTVEGPKLTKELW"
     gene            complement(79313..80989)
                     /gene="mdcD"
                     /locus_tag="IWM35_000069"
     CDS             complement(79313..80989)
                     /gene="mdcD"
                     /locus_tag="IWM35_000069"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HAP9917958.1"
                     /translation="MKNSFVELNARERVNALLDNGRELIDPFDQIIAPSLPAQGIVPE
                     SDDGIVVSRGTINGKQAVVIAMEGKFQGGGIGEVSGAKIVAALEHVLEENKKGNEIFP
                     VIVLDTGGVRLQEANYGLLSISEIGNVIIALKQYVPVIGLIPGRVGSFGGMSITSALM
                     SYLIATKKARVGLNGPEVIEQEAGVREFDSSDKDLIWNTLGSRQRVESKIIDELVTDS
                     VEAIKAAVIAAMDERKDTHRSESSAFYLSLLDSLDLSKPMAIDDYNEAIANHQAKSID
                     LPEVAEGNEPAAASVGYDWFAALTGMKNPTSSISTVYYGKSSKFIEEAIVTSIVPDPK
                     NPMYRVRNGEVGLVEGFRMGQILDHVYEEDKDKKVKRPIIVVIDVPSQAYGYNEELIG
                     IHVALANSAAAYAKLRQAGHPVIGLIAGNAISGAFLAHGLQSSRLIALNNKEITVQAM
                     SKESAARVTKRSVAEIEAAAEKVPAIAYDINNYTKLGAIYHFLEDITDQAASEQNVSK
                     VIEAINEAIQDVRNTNDTMLTNRYTNETAKTFGRVETNKVRAKMDEEWDA"
     gene            complement(80979..81278)
                     /locus_tag="IWM35_000070"
     CDS             complement(80979..81278)
                     /locus_tag="IWM35_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit delta"
                     /protein_id="HAP9917959.1"
                     /translation="MEQLAYKYPTKQPINKKIHVGVVGSGDLEILMFPTEKTESTVKI
                     CTGSDGFGEVWNNVLTRFFDRYPISADIVINDFGATPGVVYLRLTQAMEELSDEK"
     gene            complement(81380..83026)
                     /gene="mdcA"
                     /locus_tag="IWM35_000071"
     CDS             complement(81380..83026)
                     /gene="mdcA"
                     /locus_tag="IWM35_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
                     /protein_id="HAP9917960.1"
                     /translation="MEKPNRNWASKREKKAQRLEKISNLLNGKYVETEKIVEVMEKLI
                     APGDKVVLEGDNQKQASFLSESLEKVDAEKVNDLHLIMSSISRPEHLNIFEKGIASKI
                     DFSYAGAQSLRVAQMIEDGTMKLGDIHTYLELYGRLFIDLIPNVVLVAADKADKDGNL
                     YTGFNTEETPTIIEAAAFKDGIVIVQVNEVVDSLPRVDIPGDWVDVVVKADKPYQLEA
                     LFTRDPQNITELQILMAMMAIKGIYAEHGVQSLNHGIGFNTAAIELLLPTYGESLGLK
                     GKIAKNWVLNPHPTMIPAIESGWVESIHSFGGEVGMEKYIAARPDVFFVGKDGTMRSN
                     RTLSQAAGQYAIDMFIGSTLQLDYEGNSSTVTKGRLSGFGGAPNMGHNPGGRRHSTPA
                     WQSLRDNDDPLGKGQKLVVQMVETYGSNKKPVFVEELDALAVQKQAGLANAPVMIYSE
                     DTTHIVTEEGIAYLYKAKTKEERQAAIAAIGGVTPLGMTSTKESLDSLRAAGIVKLPE
                     DLGIKRSEAKRSLLAAQTIDDLIEWSDGLYEPPMKFRTWS"
     gene            complement(83033..83944)
                     /locus_tag="IWM35_000072"
     CDS             complement(83033..83944)
                     /locus_tag="IWM35_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HAP9917961.1"
                     /translation="MSTGFLFPGQGSQKLGMLADIPEMYMERLENVTGYRLVDKEENY
                     QDTVFIQLALLTKAVFYLDEMEKNGIQPDIAAGHSIGAFCAAVACRSLRFENAAELVY
                     HRAVLMKEAYPSGYAMGVVVGVTRSEAEEIVAKTFDPDHPVYLSNENCPMQHTLSGSI
                     EGLSVTLAEAKRHQAQTAKLLKVPVPSHCLLMNDTVKKFKPYMQSVHLSDARCLYIKN
                     TDGRATQDAEEIRKDLLENLAFPVQWNQMMDLAKELGMDLTIEFPPGNTLTKLIHTKF
                     GVDKGIRTINLDQHGIDDATFLYQKWR"
     gene            84064..84921
                     /locus_tag="IWM35_000073"
     CDS             84064..84921
                     /locus_tag="IWM35_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP9917962.1"
                     /translation="MKLLHLQYFKKVFETKNVTQAAKELFISQPALSRAIKHLENELG
                     VPLFYHSGRNIETTVYAEEFYPYAVKTLDTLEEGIKLLSSVNEKIVTSVILYLEVASV
                     SIPNLVRIFMEKHPDIQLTIMQHHLPDDITDPVLYITSEMKPGMTNIPIMKEPVYVAI
                     PKKHPLAKKEHLQLEDIQRTPILMLSKSNAFRHTLDAALEAKSIELTISSTTDDPATL
                     RSIVKQGLAVSFFPKVSWSYDKNDPFVLREIKDLPLTRTIYLSSSFTEDHPLIKMIAK
                     TMREFYLGK"
     gene            complement(85108..85470)
                     /locus_tag="IWM35_000074"
     CDS             complement(85108..85470)
                     /locus_tag="IWM35_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9917963.1"
                     /translation="MLTKKELIDHINETYNLSPEYIFKKFPDYCVFRHQKSKKWFGVL
                     MSVPEEKIYDDASDHRIINILDLKVDPELGGILRGKEGYFPAYHMNKEHWISIDLRKI
                     EKLDELADLIDGSFKSTQ"
     gene            complement(85659..85841)
                     /locus_tag="IWM35_000075"
     CDS             complement(85659..85841)
                     /locus_tag="IWM35_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9917964.1"
                     /translation="MIFFISEYINQTPFYSKNTEKINGGQVSTDFSIIVYFSGAFTDD
                     KNFIMNLKNDTTKIKK"
     gene            85816..87255
                     /locus_tag="IWM35_000076"
     CDS             85816..87255
                     /locus_tag="IWM35_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP9917965.1"
                     /translation="MYSLMKKIITEKDIRRHVSLVEQLLNHTKITVNELAEIIGTTER
                     TIFSDLQSIRSQLPEGWDIISDQAGISLQNQQNLLTNDLWEIFFKQSVSVELLKNLLF
                     TKKVAVPDFLADHGLSYGTLKRHVTKINQRLASYDLQIDLTKYTACILGKERAIRTFY
                     HRLLIPFTHNNYFFEDYSIHESHYFQFLRNLSQTELAVETEEIFGTCWFFINTIRIKA
                     NCRLDSSIHTNSTLSSLYDSALKKLYLTEGIYLKDTELSFASFCFLESWNYNNNYGQE
                     TARCLHHSPFLEVLETFVEELASELSLDQLKKTSLTDNLALLILKYHESPILIEALDR
                     QYHRFLETYDQQYAHLYQQKENLLNDLKKEIEINEPNYFLQLLSLLIQQTIFSIKPNL
                     FNVYFIFQGEPSWKAFLQQELKDYLGKRVCFLPIELTDLSTISFEKTDILISNFPLDS
                     LDIPIVYISSIPTKNELNRLTELTFKTFL"
     gene            complement(87262..89814)
                     /locus_tag="IWM35_000077"
     CDS             complement(87262..89814)
                     /locus_tag="IWM35_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HAP9917966.1"
                     /translation="MNWYRLTVEDVLKNYQTSTEGLSQEERQKRLTDQGYNEIKARQQ
                     TKRWKKIAKHFTDLLMIVLIIASFLKFASSEYIEGSIILFVVIVNGLVSYWQERKAEE
                     SLNGLKQLMGQEAIVLSNGMQEKIPAKELVKGDVIVLRAGDVVPADIRLIEAHDLLVE
                     ESILTGESEASEKSHAVLTEEESIGDQKNMAFSGTLVQSGSAVGVVVETGNTTEIGKI
                     NQALQSVEQQTTPLIKKINRLNKQIFQGILCLSLFLVIFTTFRYGMDWHILLSATIAL
                     VVSMVPEGLPAVLTMILSVGVHEMAKEKAIIKGLPSVETLGSMTVICSDKTGTLTKNE
                     MTVVDVAAKEEACVLSIMKNCQELKTKEEQKTENLSGNPTEVALIRHTETANLPLKKI
                     EAKIPFSSEYKYMATMHAEEKGAVIYVKGAPEVLFAKSTLSSAEQEAWSQTAAEFARK
                     GQRVLGFAYKKVDSKQELTHETLTQLTFAGIAGLIDPPKESAVKAVKECQQAGISVKM
                     ITGDHKDTAKAIAEQIGLKHTAKVLEGIDLDLMSDEELIQQVPIVDVFARTTPEHKLR
                     IVKALQKNGEIVGMTGDGVNDAPALKRSDVGIAMGIKGSEVSKQAADMVLGDDNFHTI
                     AKAVKEGRRILDNLQKTINFFLPTALAQGLILIWALMLNRPLPLSPVQILWVNMVTTI
                     TLSYALGFESPDPEIMKRPPRDPKQGILSGYHLFRIIYVSLLIMVPAYLLAMQFEGQS
                     LQQTILLQNIVLAQAVYMINCRELSKPSLNQGLLQNKALFLSLGILALLQGMVIFLPV
                     GQQLIGTVSLTMTQQLLIGANVILLFLVVEIEKGIMNKLFRKKEKTAREYSR"
     gene            complement(89928..91259)
                     /locus_tag="IWM35_000078"
     CDS             complement(89928..91259)
                     /locus_tag="IWM35_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP9917967.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYGLMRAMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKENFGQIDELLPFGFAGMVVSFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKKAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQVIH
                     CQGGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFAGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHVQFIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIWFVFLNFVYKRISNRNIS"
     gene            complement(91225..92703)
                     /locus_tag="IWM35_000079"
     CDS             complement(91225..92703)
                     /locus_tag="IWM35_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP9917968.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITLLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLENPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            complement(93008..94843)
                     /gene="tdc"
                     /locus_tag="IWM35_000080"
     CDS             complement(93008..94843)
                     /gene="tdc"
                     /locus_tag="IWM35_000080"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HAP9917969.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEKVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     GQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKKIAKAMEKKLNEIIQ"
     gene            95313..96740
                     /locus_tag="IWM35_000081"
     CDS             95313..96740
                     /locus_tag="IWM35_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAP9917970.1"
                     /translation="MIEEYIEKNILRQLFLCGQFYVNKEVNLEKLSNLLHVCKTTLLN
                     DINNIKKEFEEQIAYTHREKDRYTLYFSEHIPRCKIMQQLSQNSLFLKTCLLYLEEDE
                     PDYLQLTECEFISVSKAYSLKKQVLAYFNDCGIEIDRYSPRFTEMERRLLLLNVSYRL
                     GGFNSWELPESFFERADRFIESVTENSGRFYDKENKEILSIGFAISFLRQQVCAVTID
                     SKFIEEIKKRPIYNYVESAWENTDFQTYYKKEEFAFILTLFNLCNYGFHSYQLIAEDF
                     QQLHQVFIDNTPEIKELVATFESHFNQELFGNQPFERALIHLMRSAWDNYQLFMPEKF
                     YLLNEEQTNLYKEVQTIFSSWSSQLPYDLRLNPNCMRAFVIELSGILRLTKEHLTIYI
                     VTNSDVHYLIYREALEAVTTFDFQVAPTIYSSISDIKKYAQQSSNRVLCERTLYTPDA
                     VQYENIIPISINTIDRAIISAVQNK"
     gene            complement(96832..97197)
                     /locus_tag="IWM35_000082"
     CDS             complement(96832..97197)
                     /locus_tag="IWM35_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HAP9917971.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQSDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPLDVQNK"
     gene            complement(97194..97559)
                     /locus_tag="IWM35_000083"
     CDS             complement(97194..97559)
                     /locus_tag="IWM35_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HAP9917972.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
     gene            complement(97751..97999)
                     /locus_tag="IWM35_000084"
     CDS             complement(97751..97999)
                     /locus_tag="IWM35_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9917973.1"
                     /translation="MKNASLFSLSISKDSSFLLECQMIGREEKGNFDKKEEVSDFPVN
                     KNRVGKKKRFEEVYGSIFRNLGTKKAPRQCFFEKLMTD"
     gene            97998..98126
                     /locus_tag="IWM35_000085"
     CDS             97998..98126
                     /locus_tag="IWM35_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HAP9917974.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            98183..99877
                     /locus_tag="IWM35_000086"
     CDS             98183..99877
                     /locus_tag="IWM35_000086"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HAP9917975.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKITNTLTQ
                     EEEYVVVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
     gene            99944..100150
                     /locus_tag="IWM35_000087"
     CDS             99944..100150
                     /locus_tag="IWM35_000087"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9917976.1"
                     /translation="MFLIWLDLLPQMQAFEHRLPRGYEKAALHQVIRAAKLKQLLMFL
                     TWLDLLPQMQAFEHRLPRGYEKGV"
     gene            100528..101661
                     /locus_tag="IWM35_000088"
     CDS             100528..101661
                     /locus_tag="IWM35_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HAP9917977.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNDLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQSGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYA
                     MIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP"
     gene            101692..102012
                     /locus_tag="IWM35_000089"
     CDS             101692..102012
                     /locus_tag="IWM35_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9917978.1"
                     /translation="MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLL
                     TGIFYIFGSLASLTKIPDRYGTYFYLAGAIFLTIRPILRIVRNIFIYDEKKAMKRTSK
                     KKEA"
     gene            complement(102041..102505)
                     /locus_tag="IWM35_000090"
     CDS             complement(102041..102505)
                     /locus_tag="IWM35_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9917979.1"
                     /translation="MVTYNWIKLREHPELVKQSANWFSSKWGVPAVVYEESIRTGIAN
                     KESIPQWYVVLDEKETIVGGAGVIENDFHNRPDLTPNLCALFVEPEKRKQGIAKRILA
                     EIRKEMHQLGYEKLYLITDHTTFYERYEWTFLTMVKEESGTTARLYQSDTIG"
     gene            complement(102637..103890)
                     /locus_tag="IWM35_000091"
     CDS             complement(102637..103890)
                     /locus_tag="IWM35_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP9917980.1"
                     /translation="MDKFVAFMEKHFIPVASKIGAQRHLVAIRDSFMVSMPLMILGAL
                     AVMINNLPIPGFQELMNSIFGGESWKGFGAAAWNGTFAILSVLIAFLLAYHLANGYRK
                     DGVSAGVISLGSFFALGGALGMSSNGLFIAIIVGIISTEIFVRLSGNEKLIIKMPDGV
                     PPAVAKAFASLLPAMITISAFALVAAIFAAFGVDDIVGSFYTVVQEPFMGLANSYPSA
                     LLLAFITPFLWFFGLHGANMVDPLMQTINAPAIEANVNAIAAGHSAPFIVNKPFFDSF
                     VNLGGTGATLGLLLAIYLVGRKNKPYMVVTNLSIAPGVFNINEPTMFGLPIVLNPIMF
                     IPFILTPMVLVSVAYFATSTGLVPAATVMPPWVTPPIIGGVLATKSIAGGVLAAVNLV
                     LSILIYLPFVKVATIQERRKEALDA"
     gene            complement(103919..104236)
                     /locus_tag="IWM35_000092"
     CDS             complement(103919..104236)
                     /locus_tag="IWM35_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAP9917981.1"
                     /translation="MEDQKNLEAIMGLIMFGGNAKSDAMEAIAAAKKGDFTLADEKIK
                     DAEESLVQAHHSQTEMLTQEAQGNHVEVTLLTVHSQDHLMTSIAFTDLAKEIINLYRR
                     LNA"
     gene            complement(104262..104582)
                     /locus_tag="IWM35_000093"
     CDS             complement(104262..104582)
                     /locus_tag="IWM35_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP9917982.1"
                     /translation="MEKKTIMLVCSAGMSTSLLVTKMQKAAEAKGMEADIFAVSASDA
                     DNQLESKNVDVLLLGPQVRFMQNQFAEKLAPKGIPLDVINMADYGMMNGEKVLDQAEK
                     LINK"
     gene            complement(105046..106461)
                     /locus_tag="IWM35_000094"
     CDS             complement(105046..106461)
                     /locus_tag="IWM35_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP9917983.1"
                     /translation="MKITIPKNFILGAASSAWQTEGWKGKKEGQDSFIDSWYKNEHFV
                     WHNGYGPAVATNFMEQYQQDVDLMKSIHLTHYRTSINWARFFTDYENCIVDEEYAQHI
                     SEMIDSLIEADVEPMICLEHYEVPAFLLEKYDGWSSRKVVDFYAKYAQIAFDRYGDRV
                     KNWFTFNEPIVPQTRCYLDAVRWPHEQNTKKWMMWNYHKVLATAKAVEIYRSGNYGGR
                     IGCILNPEMVYARSNSPEDQKAAYMYDLFYNRVFFDPMVKGYYTEEFLELCQKQSIYF
                     DPATEDLETIQHHTVDFLGINQYYPKRVRAPRYQWNEETPFHPERYYEVFDLPGKKMN
                     NSRGWEIYPKIMYDIAMYLKENYHNIPWLITENGMGREYEEQYMDEKGIVQDDYRIEF
                     IGQHLHYLIKATEEGANCEGYMLWAFTDCVSPMNAFKNRYGLVRIELNDERSRTLKKS
                     ASWYQTLIDKRELDLPDEIYK"
     gene            complement(106478..107794)
                     /locus_tag="IWM35_000095"
     CDS             complement(106478..107794)
                     /locus_tag="IWM35_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP9917984.1"
                     /translation="MRGSGMSFMDKLANVLGNVATRINNLRYIMVIKNAFAALIPVII
                     TGAFGTLFSAMVFDSENGLAQISFLRFLEELKPIASAISYVTLSFLTIYAVFLIGIEL
                     AKLNKVDGVFPGIIAVMSYLSVNPTVYELVNENQTVIVENVLAKQYTDTKGLFLGMLV
                     SILSIELYCWLRKQKKLQLRMPDTVPTNVSASFSALFPTILTVSGIAAAGFIIKELTG
                     MYLYDIIYNVVQRPLEGVVQGLPGILLLMFIAQVFWVIGIHGNQMVKPIREPLLLAAI
                     AVNTEAFEAGKEVPNIITMPFWDMYMSMGGSGVTIGLLIAVLLVGKREDMKQITKLSL
                     APGIFNINEPVIFGMPIMLNPILAIPFILTPLITGTIGYFATALGFAAKAVVMVPWPM
                     PPIVNAYLATAGNIGAVITQLVCIIVSILIYLPFVKVANRVENDGI"
     gene            complement(107944..108642)
                     /locus_tag="IWM35_000096"
     CDS             complement(107944..108642)
                     /locus_tag="IWM35_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HAP9917985.1"
                     /translation="MEENKTLVEVTTDKILRYLKEMQLQVGDKLPNEYELSDYLEVGR
                     STVREAVRALASRNILEVRQGAGTFVSDKRGIANDPLGFSLIEDQDRMIRDLFELRYL
                     LEPKMAGLAAEYATKNQIVQMKKLTQKIETSFREGTQEHVQLDIDFHTMIAEASGNVA
                     LLHIIPIINESIALFNKNYDIQHLKIETIDIHTEIVQAIERRNALAAFDAMTVHMAIN
                     RRELQKIIGEKETN"
     gene            complement(108931..109920)
                     /locus_tag="IWM35_000097"
     CDS             complement(108931..109920)
                     /locus_tag="IWM35_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HAP9917986.1"
                     /translation="MYIADSARYEKMEYRKSGHSGLKLPILSLGLWQNFGDYDPIHNQ
                     REILRGAFDMGITHFDLANNYGGPAGAAEKNFGRIFREDFQAYRDELIISSKAGYHMW
                     EGPYGEWGSRKSIISSCDQSLQRMGLDYVDIFYHHRPDPDTPLEETAEALMQLVRQGK
                     ALYIGISNYNGEDTKKMTEILKRKEAPFIIHQMRYNMFSRALLEDDLSPVLEEEGLGA
                     ITFSPLAQGLLTNRYLHGIPEDSRAHRKEIPFLSEEQVGSTLEKIKALQTIAVSRGQS
                     LAQMALAWNLRQKSVTSVLVGASRLSQLQESVRMMDNLDFSPEEELRIDQILE"
     gene            complement(110000..110833)
                     /gene="msrA"
                     /locus_tag="IWM35_000098"
     CDS             complement(110000..110833)
                     /gene="msrA"
                     /locus_tag="IWM35_000098"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAP9917987.1"
                     /translation="MDEEQIFIQTLYNLILNPNTRDWERKVLIQTKNDIRENISVKEQ
                     LSKLEATLRPLAIRMNLTPDVMDFYLLITEGFDKEQKYDFSKHAMQDADYQERAVFAG
                     GCFWCMVEPFESKKGILSVLSGYTGGHVEKPNYDQVSGGYTGHVEAVEIIYDTREISY
                     SELLTIYWQITDPTDTFGQFQDRGKQYRPVIFYQVERQKELAEQSKQKLDSSGTFHQP
                     IVTKIEPAGTFWPAENYHQQFYKKQPKRYKKIQQARNQFLIYQRVKNKWQKNIRKNHF
                     D"
     gene            complement(111008..112642)
                     /locus_tag="IWM35_000099"
     CDS             complement(111008..112642)
                     /locus_tag="IWM35_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9917988.1"
                     /translation="MKINKKVVILSGLIIVSSIYSFIFFKNTQSIYTTGDLSFHLSRI
                     KGLSTIFTSPINYETFNYTGYGVNYFYPFLTFFPAVMLYWMTKNLIVSYIIYVWLLNL
                     CTTLVAYHYGERFLKQKKAAFLFSCLYIFSAYRTVDIYYRSAIAEAIAITLVIPVLFY
                     AYQIISGKEEKYPSVKLALSMSLLVYSHVLSTLMSTALIIIFIFIRLVSKGFKNADFI
                     AIFKKLFSAAGMTLVLTSAFWYPMFEQMLYQKINKPSVTNLYAHASNVFDSLTEAMNN
                     DLTTYSMGLVGLLSLCIPLILFKKLTNIEKKIYYGTCLTWLATTSLVPWYLLQNTPAK
                     LLQFPWRILSLQIIFSSLILTMIFFKNRRYNKTRELFYLFMTVLLLITLTVSSKKNFN
                     QKIISQPGHIVVNKETVEAYTTKGMDYFLYDYSPIASLENKEHIIKHEAKIGSQWVNL
                     PYKTTGSSIIYRVTNEKSQVITLPVYAYLGTNVEINHQPVKEQVNANGLVQISGVKGV
                     ADIKVSTSYTVTTIISYLVTLTAYLCLFFKIVFHKR"
     gene            complement(112629..114476)
                     /locus_tag="IWM35_000100"
     CDS             complement(112629..114476)
                     /locus_tag="IWM35_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9917989.1"
                     /translation="MFFFIFFSYLKVIKIDSFLVYHTSAMKNLTQNLLKKDNSSILKI
                     ELKKENNKSMKISKKAFLLVVLILLSTLYSVNFMRNAQEIYTTGDLSFHLSRIKGLSS
                     IFEGPINYTTFNNYGDGLNYFYPFLTIIPAVVFYGISNNLILSYVLYIWLLNICTILI
                     SFYYGEKFFKKIDAAFLFSCLYTFATYRTVDIYYRSAIAEAIALTIIPAVLYYAYGLI
                     FNREKKVIALSVSISLLVYTHVLTTLLCSALIACMIIVRLFLSKQIKNDIKFVVVNLV
                     KAVFLTCALTSYFWYPMLQQMNYQEIHSPFKTDLQLEALSISDSIIQALNNDITTYTM
                     GLLGLVALIFPLFILKEMEKKEKIIYVGVVSTWVCVTNLFPWFLFQKTPISLLQFPWR
                     ILGIQIFFSSMIITLVFMKKTMNKKYSWLIIGATVVFLLVTSIAAKENYAKVIQSKHG
                     HIVINEETLPRYTTSDMGGLYDYAPTEAIKERPHLEKHEVKVGESWEKGKYKAADNNI
                     TYTVASNKKQKVTVPVYAYLGTQVKVNGKVVNDSYKKHGLVNVDVNAGENKIEITTKY
                     TPTALMALWISILTYCLVVYSSFKRFAPNKLKDSRYVTKKEINKKNENK"
     gene            114463..116100
                     /locus_tag="IWM35_000101"
     CDS             114463..116100
                     /locus_tag="IWM35_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HAP9917990.1"
                     /translation="MKKNKNQIIDIIFMLFLSFAYVIPLFMSKGIYHSVNQDTYFHLS
                     RIIGLDNVWSSPVNFNNFDHHGTMMNIFYPWLTLYPAFLFYKMMGNLVLSYNIYYFFI
                     TFLTMVVSYFSMKQIKNNRYISLLFSIIYTFSAYRAIDIFHRASLGEAVALTFLPLIL
                     MGCYEIYIRDYQKWYWLSIGMTLVVYTHLLSVAMVSVFIGGTLFLSFYFWDQKIARLL
                     SLLKATVLTFFLSAGFLIPFIQQSRAQELKVPLGKELSGMAPSDMLTHILNNNYNNYT
                     IGLFLFLGLIGAFIFIKKLTADDLFIFFLGVFVLFCSTNLFPWQLFNHTPVKSLQFVW
                     RLNGFSSLFIAYTMSIVIYYIFSTKNNSWKIMVFTFLALILHLSGVSNSIHANKDSLT
                     LIAPEDAVAIAENYNHTDYANKESIYHPDMINNDQYLLGNQEINPTTHFMSNKLTIEL
                     DNSNNKNTVLTTPIYRYKGQVASINGKLVQTKLSKFGTTELTIPPGINKVVITYQYTK
                     LAIASRYLSIVTLILFLLYRFTFSKQKQPRREVQHSH"
     gene            complement(116245..116985)
                     /gene="truA"
                     /locus_tag="IWM35_000102"
     CDS             complement(116245..116985)
                     /gene="truA"
                     /locus_tag="IWM35_000102"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HAP9917991.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNTEIGLAEMIDFMNRYLPRDIVIKRIEEMPERFHS
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLVGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYR"
     gene            complement(117176..117811)
                     /locus_tag="IWM35_000103"
     CDS             complement(117176..117811)
                     /locus_tag="IWM35_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HAP9917992.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EVSSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTAAIAFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWPLLNKIGFCQRISFFFNYVPIARLSILHLKQ"
     gene            complement(117958..119199)
                     /gene="sstT"
                     /locus_tag="IWM35_000104"
     CDS             complement(117958..119199)
                     /gene="sstT"
                     /locus_tag="IWM35_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HAP9917993.1"
                     /translation="MLEKFLDMSLVQRIVVGILVGILFGIFFPTWTFISILGTLFVSC
                     LKAIAPILVFFLTMASIAKHKIGNKTFVKPILILYLVGTLLSALVAVIVSSIFTVPIT
                     LQETVSEKAPQSLESVLSTMLTNVTQNPVQSLIDANYLGVLFWAVLLGLAFRARSEST
                     KELVDQISIALSQVVQVIIAFAPIGILGLVYQSIATTGIAGLAEYLQLLLVLIGTMLF
                     VALVVYPFLTFLFIKENPYPLIFFCLKESALPAFFTRSSAANIPINMMLAERLKLTKE
                     SYSISIPLGATINMGGAAITISLMTLTAVHSLGIEAPFVLKVLLCILSALAACGASGV
                     AGGSLLLIPLACSLFGISNDVAMQIVGIGFIIGVIQDSMETALNSSSDLLFTAIGELG
                     ARKRSGEKIKLKDCLDGLSER"
     gene            119397..120395
                     /locus_tag="IWM35_000105"
     CDS             119397..120395
                     /locus_tag="IWM35_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9917994.1"
                     /translation="MFLSNFFVRCLFLKKIKKESYGFLTIFVACIMMVLQRILISAVT
                     IPTYATNPGRFYIYTFLQTSVVIGANLIPLYMGYQYQKIARLKVLHYLSRFAFIFLIA
                     TLVCNIFFYVQAGSLNIRDYWLILTPISQNYFTYAVSCTLLFCSIPYTVGFLNKLSKT
                     TIKYLLLFLTIGLVGCSTLFNKDPFALQNGQSILWIFYLFLMGYGLKEWNWKEKVRYK
                     LPQVFISLIVLFGLIILMTQISLIIRGNTDTALRFSKPYSLFAMYYTFSSFLFLEMLS
                     DKLGIKGRGNFLAVFLIVTQVICNFPLVSYRVSTFMKREFPNSGKAWLINIGQFFI"
     gene            120408..122426
                     /locus_tag="IWM35_000106"
     CDS             120408..122426
                     /locus_tag="IWM35_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HAP9917995.1"
                     /translation="MSLLVIFLFYIQKIRFIHRFIEYFAFDSIEQLTQRWKKRIHWLF
                     VHKSYFLVAAFFYCFTFVQIFVLEPKEGWKETIQVALKIFTQRQAPMILTIIIIMGFF
                     FLLLLISNRFWYALTATLIINLLLTISTVIKMEMREEPVFPSDLKMLTGLSELLSMVS
                     PVLLIVGGLILLLLLITSIIVQRRLQKQYSLKIHWKRRFISIAVLLGCFSGVFFINHK
                     NSPSFLLFNLFKVNKTFFNQQGAVKDNGPVIQFLNNIDIKIMEEPAGYSKEKINGIMK
                     KYNTKADEINQTRSDWLNNQTIVLNLSESFSDPARVPNLTVPTDPIPYIKSIITEGTG
                     GLMLSVGYGGGTANMEWEGLTGLSISNLSASLVTPYTQLVERQKISPNITDLFDEKIA
                     IHPFTAALYRRKQVFDKFGFDRFYYLDSPDKLTYTDKIEQSPRISDDSAYKETLKALQ
                     SNTETSQFIQLSTMQNHMPYDDYYSELNYTAEGTAVIPNRRKELQTYMQGLHYTDTAV
                     KKFIQEIDKIDKPITFVFYGDHLPAIYSGNDSKKYGLEQHQTDYFIYNNKYSREQAAT
                     LNKEVVAPNNFGAMALEHANIKVTPYYALLTEVSNHLPALTIDPTESLSNRFNGKQVF
                     VTEDSQIIQEKNLTEKQKEILEDYRLIQYDLVAGNQYSATWATQKIKD"
     gene            complement(122486..123202)
                     /locus_tag="IWM35_000107"
     CDS             complement(122486..123202)
                     /locus_tag="IWM35_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HAP9917996.1"
                     /translation="MKKRILVTIGLAFLLAGCTNGNESKKESTKETAKATVATTTTTS
                     TESKSTAASTETADTSTSSESSKTTETTQTSTSSSSVEASSSSSESSEQVEQTLWNAD
                     KSAQLEAFMTKWGQTMGQQYKSYTNQMSVDLYGLKVPQAILNGEWKMAIGGVPVSAEW
                     SESGTGQADYQITAVYSDAETEPYLKKHVYLFGFQQNQPKVLVTQQNQGNPDNYLYFN
                     ETANNELKNGFNQIVYGQAN"
     gene            complement(123429..123971)
                     /locus_tag="IWM35_000108"
     CDS             complement(123429..123971)
                     /locus_tag="IWM35_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAP9917997.1"
                     /translation="MNIVEEKFVQETKQADIDHHKPTAGAMVGHVLANFKLEEMKLIQ
                     AQLYVLGTSSQELSTLFSEYADQEARWFNKISHELVIENEIVPSTLEEVMRYGKIYEH
                     GQNKYLPASTMLENFAKDFDFQNVFITRAIKLAEKEEKYGLQQLLVELLSFNKEIIYQ
                     VQRLQGKTVRQDLDEEDDDD"
     gene            complement(123976..124203)
                     /locus_tag="IWM35_000109"
     CDS             complement(123976..124203)
                     /locus_tag="IWM35_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance protein CopZ"
                     /protein_id="HAP9917998.1"
                     /translation="MKKIIMQLDTLACPSCMQKIEHAVSQQPGVSNTKVLFNASKIKA
                     NFDPDEVHPEQLAAVVEKLGYEVQSAKVKEA"
     gene            complement(124282..126162)
                     /locus_tag="IWM35_000110"
     CDS             complement(124282..126162)
                     /locus_tag="IWM35_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HAP9917999.1"
                     /translation="MKLQKWIQEHRNHITALSAVLIIFGFIGKFTGFVLFYNIALAIA
                     SIIAVIPIMIHAYQAVKVKVISIDLLVTIAVLGAFMIGEYKESAIVTFLFLFGSYLEQ
                     KTLTKTRESIKALTEMAPSTASVVQEDGTTEEVEIDDVEEGDIVLVKTGGAIPVDGRV
                     IDGKGYADEAAVTGESRQVVKISGDEVFSGTILADGYVTIEATKVGDDTTFAKIIELV
                     EEAQDTKSHAEKFIDRFSQYYTPTVLLIALVVGLISRDFRLAITVLVLGCPGALVIGA
                     PVSNVAGIGNGAKRGILVKGGEAMDTFSKIDTFVFDKTGTLTKGTTAVSTIKNYTEDE
                     GDSLALVARLETLSDHPLGRAVIHYAQEKDIDFQQLAVKDNQTIKGQGITAEIDGHTV
                     CAGNTKLIAAHKLSLTPKQVQDLHQLQQTGSSVIIVAIDKKITQIIGVSDVIRPEVAE
                     QLAKLKQAGAKQLVMLTGDNQMTADYVADMLGIDEVHAELLPDEKVQFVKKYQEQGLR
                     VAFIGDGINDSPSLAAADIGIAMGSGTDVAIETSDVVLMQSNFESLVHAYRLAKKTVL
                     NTRENVVIAIGVVLFLLNGLFAGFIYMASGMFVHEASILIVIFNAMRLLYFGHESKEE
                     KS"
     gene            complement(126351..126965)
                     /locus_tag="IWM35_000111"
     CDS             complement(126351..126965)
                     /locus_tag="IWM35_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9918000.1"
                     /translation="MKLTFKPLEKKDYKKAIYFAVEGMNFERYMENELLLNLYGRYFL
                     YMELNRATQILALYAEDELAGVLLAEIKGEKKKHRSFGQQIYVKIFDFLQNIFAKESK
                     GAYETANEQMLAAYLKKHSPDGEIIFLAANPDLKTKGIGTKLLTEFEKREKGKEIFLF
                     TDSGCTYQFYERRGFERLKEKISKIKILDKEIDLRCMLYRKIIQ"
     gene            127107..127667
                     /locus_tag="IWM35_000112"
     CDS             127107..127667
                     /locus_tag="IWM35_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP9918001.1"
                     /translation="MKKTDRRVKKTEKALAETLSTLLVNKKIQAITIRELTETADVHR
                     STFYTHYKDIYDLYDQLESNFFRDLNEILSYDPAHSYEELYTRLIDYVYANTFLYRLF
                     ILNHGDISFQKKVKQVIEENYLNIWLYEDKKTIITEEMRFFTTYHVQGCLSVLNRWVE
                     SNYKYSKKELLELLQKLNDHIEKIMP"
     gene            complement(127972..128286)
                     /locus_tag="IWM35_000113"
                     /pseudo
     CDS             complement(127972..128286)
                     /locus_tag="IWM35_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
     gene            complement(128540..130175)
                     /locus_tag="IWM35_000114"
                     /pseudo
     CDS             complement(128540..130175)
                     /locus_tag="IWM35_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294751.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(130730..131293)
                     /locus_tag="IWM35_000115"
     CDS             complement(130730..131293)
                     /locus_tag="IWM35_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HAP9918002.1"
                     /translation="MITVVHKERQEKSSSDVRLWVYLGSISTKIQYGYTDSAKKQGNV
                     KFLRITDIQEGRVNWSSVPYCDISNSKLVDLRLEENDILIARTGGTMGKSFLVKEISE
                     ESVFASYLIRIRLVEKLLSEYVDCFLDSPLYWKLLEKISYGTGQPNVNGTNLSKLLIP
                     LPPLEEQQRMTTKIEMIRRSIRRINFE"
     gene            complement(131308..132351)
                     /locus_tag="IWM35_000116"
     CDS             complement(131308..132351)
                     /locus_tag="IWM35_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9918003.1"
                     /translation="MTQRVAIMILVLLVIGLLVYYVLKFKHWKQQRIHQDIEKKLKRY
                     PIVQAAWEKAEAKQYNIPGLTETRMVVPETGENEVCQWMTPQGLAFSQDFVFISAYCY
                     DHQHHSIIHVLDRETGQPIKLLILPKRPHVGGLVYDTKRELLWLTITGSATGRVAALR
                     LIDILADTSEETGQPIAYWLTTDLSEIPQASYLTQNNDQLVSGNFTLKGEGQLTFYLL
                     PTIAEMKTAIRRKDKIQPTVTMSRKTSDKIQGVAFYGHYLLASRSYGPYTSELLVSER
                     DSPSRILKRIKFPPYLEQIVVVEDRLAVLFESGAAAYREKANPVLANVLLLDLATLLH
                     ANKRPKKIAATKK"
     gene            complement(132484..132888)
                     /locus_tag="IWM35_000117"
     CDS             complement(132484..132888)
                     /locus_tag="IWM35_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HAP9918004.1"
                     /translation="MKKMYETAMINRGGRDGEVEAPNGSMHMKIDRPGIHSEGTNPEQ
                     LFAAGYASCFNGAVQHMLEENNLESDSEVKARVSLFQLEDGGYQIGVVLEVSLPGLEK
                     DQAEKIAEEAHAFCPYSKATRGNIDVEVTIVE"
     gene            complement(133121..133756)
                     /locus_tag="IWM35_000118"
     CDS             complement(133121..133756)
                     /locus_tag="IWM35_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HAP9918005.1"
                     /translation="MIFLQKYGFYFLLLGVMSDLSTPYILGLFYPKLNQMTTVISVFG
                     DVDSPVRRAFLVWSVVSGLFFVLSLPALYHLFVGTSKTLAILVVATVGLYGIGDCIFT
                     GLFSINTNESSCNLSTWIHNIGSGLGYAGFLLFPLLLVLLYRQSGQGTLSHFYLVLTV
                     ISLLIAGLYGLARIPSISQFAFFKQLGFFQRLSFFFNYLPMICLSFFQLRK"
     gene            complement(134022..135074)
                     /locus_tag="IWM35_000119"
     CDS             complement(134022..135074)
                     /locus_tag="IWM35_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP9918006.1"
                     /translation="METSQKISPKIFLNKVLAGTATGIIIGLIPNAVLGAILKYFSAY
                     PFAVTIAQLAVIFQLATPLIIGGLIALQFGFDPMRMMVVAGASFVGSGVVKFTPDLGE
                     TGVYVGAGTGDIINTMLTASIAVGLILLIGNSFGSVSIVLTPIVVGVGAGLLGFYLFP
                     FVTAITSAIGSLINNFTTLQPLLMSILIGCSFAFLIISPISTVAIGMAIQLDGVSAGA
                     AAMGVAATTFVLVVNSWKVNKSGVTIAIALGAMKMMMPNLFRKPVILIPCLVSAVITA
                     IPVALFSISGTPASAGFGVVGLVGPLASLDAGLNGFLVVLCWLVIPVFAALITQFLCE
                     KVFKLYDREEVFKFLG"
     gene            complement(135087..136025)
                     /locus_tag="IWM35_000120"
     CDS             complement(135087..136025)
                     /locus_tag="IWM35_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015081805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HAP9918007.1"
                     /translation="MKIAIAGAGAMGSRFGLMLHQSGNEVLLIDGWAEHVQQIKEHGL
                     QANFNGKEVEAKLPIVLQSEVEKEDQVDLIILFTKAMQLEKMLQDIQSLIKKDTEVLC
                     LLNGIGHEDIIEKFVPMENIYIGNTMWTAGLEGPGQVKLFGSGSVELQNLGDGKEAAA
                     KKLADKLSESGLNAHFSDNIHYSIYRKACVNGTMNGLCTILDVNMAELGKTSTAHKMV
                     ATIVNEFAKVAAVEKIELDVPEVIAHCESCFDPETIGLHYPSMYQDLIKNHRLTEIDY
                     INGAISRKGKKYGVATPYCDFLTELVHAKEDSLNVK"
     gene            complement(136476..137126)
                     /locus_tag="IWM35_000121"
     CDS             complement(136476..137126)
                     /locus_tag="IWM35_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9918008.1"
                     /translation="MNDEYKNDEDKMLFEEIENRCRLNFELRGKMSLIQQKKYLANKS
                     EFTLGHVEKLISDWISSRSEFTKIKQPIKFDMKKLLLNKSEIGNRDQYIRAKGQEIID
                     SLGEMRSYNYLYVTHRADGMVITVGKSSSNDIFLDGDLFYQLNINHLSGTENIILRTE
                     YGNEIFAKYDEILKNYLDWAWIIPVESGDAKKLERLLGDELINKKVPILNYYSHRQ"
     gene            complement(137226..137735)
                     /locus_tag="IWM35_000122"
     CDS             complement(137226..137735)
                     /locus_tag="IWM35_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HAP9918009.1"
                     /translation="MNNNDRLLRLRYAIDLKDTDLIKAFELGGVPLTKEEAQAVLTKV
                     QDKNKDNAENNVYEKTINNQVFDAFLNGLILLARGPQKDKPIVDTPQKSKEIKYINNV
                     LLKKLKIALSMTTDDILDVFAEAEIYPSKGEIGAFLRKEGQRNFKPCGDKYMRNFLKG
                     LGIYNRRKV"
     gene            complement(137936..138805)
                     /locus_tag="IWM35_000123"
     CDS             complement(137936..138805)
                     /locus_tag="IWM35_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP9918010.1"
                     /translation="MYGSIEAGGTKFVCAIGNDDLKVLERVSFPTTTPNETMSLVIDF
                     FNHYKEQLESIGVGSFGPIDIHRESKTYGHITSTPKTAWKNFDFVGTLNKHFEIPIAW
                     TTDVNAACYGEYVSGHGKGLSSVVYYTVGTGIGGGAIQDGIFVEGFSHPEMGHTLVKR
                     HSGDTFSGTCPFHHDCLEGLASGPAIEMRTGTKGQDLSIEDPFWEIEADYIAQCACNT
                     TLMLSPDIIIFGGGVMQQEHLKKKVQRRFLELINGYVDTPNIEEYIVTPKLADNAGTI
                     GCLTLAKDVRINS"
     gene            complement(138825..139946)
                     /locus_tag="IWM35_000124"
     CDS             complement(138825..139946)
                     /locus_tag="IWM35_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HAP9918011.1"
                     /translation="MNCLNNIWLHKGEESRTISPENKNGEKGKAAMTASHLGIGRKGS
                     PSVPINAGETITLAEMEGPGILRHFWMTVTDKTIAGSFVLRDLILRIYWDDESTPSVE
                     SPLGDFFCNGFGTRCEVNSLPIVVNPTSGMNCYFEMPFRKKAKITLTNEHPEFIKHIF
                     YTFNYALTEVPDDAMYFHAKWNREPITTKGKDYTLIDGIKGTGYYVGTYFALCALERY
                     WWGEGEFKFYIDGDEDFPTITSTGAEDYFGGAWAFHKKNFGERAYAQNFNTLFMGYPF
                     QSKRDHTRDYFSAGKPNPNPVHGFGDDALPMHGLYRWHLPDPISFKENLRVTFQTLGN
                     DDIKLYERQDDISSVAYWYQKEPHEFFEKLLDREERIPR"
     gene            complement(139948..140343)
                     /locus_tag="IWM35_000125"
     CDS             complement(139948..140343)
                     /locus_tag="IWM35_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAP9918012.1"
                     /translation="MRRLIIASHSILAEGFKETLEFLTGMHNQISSLCAYVEEIDDID
                     EQIKELVDTTDEIIVLTDMAGGSVNQKFFTYISDRVHVIAGMNLPLAMSIALQLGNDN
                     LDIGAIIAEAQQQIIYMNNQKSEERIEDE"
     gene            complement(140362..141159)
                     /locus_tag="IWM35_000126"
     CDS             complement(140362..141159)
                     /locus_tag="IWM35_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010238514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP9918013.1"
                     /translation="MSEKYQVTKKDLRKANYRWLMSVCTFNYQTQQGASVTYALSPIL
                     RKLYKDDDEYIEALNNHFQYYNTQPWLAAIILGACVAMEEKQGLEAKDAINDFKVGTM
                     GPIAGIGDSLLMTMIPTIMGSIAAYMALENNPVGIFLWFILIMVIFFFRMRGFEFGYK
                     QGMKIVTEYGEKINYLTEAASVLGLTVVGSLIASVISVSTPLKFTFGEVSMKIQPMLD
                     KILPTLIPVIITAAAYYLLSKKKISMTILILLVIVFAMVMAAFGILA"
     gene            complement(141161..141949)
                     /locus_tag="IWM35_000127"
     CDS             complement(141161..141949)
                     /locus_tag="IWM35_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP9918014.1"
                     /translation="MHFTIFQIIMLTILAFIKHVDYYGIPMIFVNYAVFWGLFTGILM
                     GDIKTGLIVGGTVQLMSLGVAGFGGSSVPDYGTCAIIATAFGSQLGVEAGLAIGLPVG
                     MLGVQLDVIVKILNGFVVRKSQEYANKREFKKMNSVLWLGPIFFGLSAAIPVFIAVTL
                     GQSAVNYVLDVLPNWFMDGLTLAGKMLPAVGIAMLLRFMPTGRYISYLLVGFFLAAFL
                     KVPIIGAAIIGFALAFNVYHRAEKDAQITASAGASDVGFIGEDE"
     gene            complement(141963..142439)
                     /locus_tag="IWM35_000128"
     CDS             complement(141963..142439)
                     /locus_tag="IWM35_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP9918015.1"
                     /translation="MSIVMSRVDNRLLHGIIVTQWAPMSKANRVMVIDDSVANNDIQK
                     SSMRLARPAGMAVSIITEETAIANFKKGKYDQEKVFVIVKEPETILHLVDNGIRIDRL
                     IVGGTVVKDNALQLSSRAYASDENISTYKKLLDSGVEIVTQFVPADKAQEIQSKLG"
     gene            complement(142511..145168)
                     /locus_tag="IWM35_000129"
     CDS             complement(142511..145168)
                     /locus_tag="IWM35_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP9918016.1"
                     /translation="MEELKERIVNYVIENSEIKNKWGNNTAQKISEKLNVSRNIVSQY
                     LNEFVKDGEFGKINTRPVIFFSLPEKVPNQQIFSSSEEAEKWYDSSFRVGIERVIGSQ
                     GSLRNVIAQIKAAIYYPPNGLPILLHGETGTGKSYLANYVYKDLLANNYIEEKASFIS
                     VNCSEYANNPELFLSNFFGHVKGAYTGAEDGRKGLAELANNGVLFLDEIHALSNECQE
                     KLFQFMDTRKFHRMGDNENWYTSSCWIIFATSEPPEDKLLSTLLRRIPVKLTLPTLDS
                     RSLYERKQLIKIFFKSEEERLAKKILISQQLLDFLLNYRFSENVGNLKNIIQLTCANA
                     FYFLDDLEELYIQMYHLPQWVNFNGMKVMKRQNELKYIQMDDLEKVDEVFNINELIEY
                     LYQTWKKDTDKQQLFEKYDRYFRQKNVDIQNNSALTYPTYQEIRFRQYVKEIERDFQI
                     EFDYYYLQITSIFLNSWIYQIQLTSDDKLYSFINDINSWCSREVQLVNRFIQIGDIYL
                     TKIQKNVLVILFKLMCSKEFQQKRLALILAHGDLTATCIASTANRIVERYIYDAIDMP
                     LMTSSSEIADKINRYLQECTGYEEIILLVDMGSLEKIYLNLELDAQKKFGLLNNVSTR
                     MAIDVANQILLDLPLKQILEQVKENQNVDYIYESSNIKKDMIVCSCASGLGTSFKLKK
                     ILEDSFPRDTDLVIETCDYYSLVNENYLSELRKENNILFIVGTLNPHLEDVEFFSIED
                     MILGMNISKFSNQLNRHFNDNELKVLNNNILKNFSLTNLMEQLTILNPTKLLERVSDA
                     IYILQNDLGISFDNNTCFGLYVHISCLIERLVKQNTLEDEIYFNETSEEFQKFQTHFK
                     QSFSVVEHYYSVDIPIHEVKYVYDYVKRA"
     gene            complement(145187..145591)
                     /locus_tag="IWM35_000130"
     CDS             complement(145187..145591)
                     /locus_tag="IWM35_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit M"
                     /protein_id="HAP9918017.1"
                     /translation="MGAELNQKLFSAADNLRSKMDASEYKNYLLGLIFYKYLSDRLLE
                     QVVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYAISPEYLFNVLA
                     DQAKQATFQLNDLNKAFLASHIYGKILSAKFE"
     gene            complement(145823..146080)
                     /locus_tag="IWM35_000131"
     CDS             complement(145823..146080)
                     /locus_tag="IWM35_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HAP9918018.1"
                     /translation="MEDSPELFPMLKDRLNELTEKRRQLQIRENEILGIFQQQGEPIQ
                     VKDVQQILTSLDCFLIQSEKKQVKALYHTFIEKITFDPNQK"
     gene            complement(146205..146537)
                     /locus_tag="IWM35_000132"
     CDS             complement(146205..146537)
                     /locus_tag="IWM35_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9918019.1"
                     /translation="MPLDLSTGRKIRFSEIQYEGIGFTLIDLKNSTFEIKEGLDLKNL
                     LCADDKIKKIQLYKVPILTPTVLTMLEMENNINYIDSGSDLLIRWQVITNFLMELKKY
                     SNTQTKFS"
BASE COUNT        43962 a        30288 c        24589 g        48099 t
ORIGIN      
        1 tacatggtgt tcaaaatatt attcgccttt tgcttctttc actaattttg cgaatgcttg
       61 ttgttggaat tcagcagcta aggctttcgt acgttgttct tgctctgctt tgctcatttt
      121 tggattttca gtcattgctt ttaccatacc gtcttgaaca taagcttttg cttcactttc
      181 catcgtactt tgctgtttct tcactaattc agctaacttg gctgcttggt ccgtacttaa
      241 ttctacccat gtctctggga ttttgaatgt attcactcgg ctgtcatatt cgtgattgtt
      301 atcagcaata ttgaaccaga atttatacat gtcgtttttg tattcccaat aagtagtttt
      361 cgtcactttt tcagcatcgc cgtctgtctt ttctacttta tttgtttcgt tatccgtacc
      421 agttgctttt ggtttttctt gtttttcatt tgttcggtaa agatacactt tttcttttcc
      481 attccctaaa gctttataca aaagcatatc tgctccttgg gaatcagcac tggaaacaat
      541 tttttctgtt tttgtttccg ttactttttc cattccaaag tgattggaca aattagcgac
      601 gatcaaactt agtgaagcga cgaagattag tccgaaaatc aaagataaaa aggtttgcca
      661 tgttttttta gcgaacacgt tcgttaaagc aaaagcaagc aagctgacaa tcaaaactcc
      721 aataatcatg cattatcccc cgctttcatt agaacaggtt cttttttctt caataaaaag
      781 gcaataataa agccaagaat cccaaagatg atcgctacga taaacgcggc atggtaacct
      841 gacagtgtgg cattgattgc ctgatcttta taagctaatg gcgtattatc caatacagat
      901 ttgcttggga tatttgcttt tgtcacattc gtcaacacac tgatcaaaat agctgtacca
      961 atcgagcttg cgatttgacg gaatgtatta tttaccgctg tcccatgact gatcaagtta
     1021 tttggcaatg cattcatacc agaagtcgtt accggcatca tgaccatgga gatcccaaac
     1081 atacggatcg cataaaggat cacgatatat aaaactggag tcgtttttgt caagaaagca
     1141 aatggtgcag ttgccgctgt caaaatgaac atccctgtga tggctaaacg tttcgcgccg
     1201 tatttatcga aaatcgcacc cgtcacaggc atcatagccc ccatgatcaa tgcacccggc
     1261 aacaacatca agccagaatg gaatgctgat tctccacgaa tattttgaat atacaatggt
     1321 acgaccattt ctgcccctac catcgccata tttgttacac cagctaaaac agctgcaatc
     1381 gtgaatgtag atgatttgaa tacacgaagt tccaagaatg gatgttccaa atgtaattga
     1441 cgccatgaga acaaggcgat gaaaatcaca ccaacaacta agaaaccaat tactttcaag
     1501 cttccccagc cgtcatctcc tacactagaa aatccgtaaa gtaaactacc gaatccaatc
     1561 gttgataaaa tagcggataa aatatcaatg ctaggatttg acaatggaat aacacttttc
     1621 attaggaaga atgctaaaac caagacaaga acaacgattg gcagtaccat accaaacaag
     1681 tcacgccaat ggtagttatc aatgatataa ccagataatg tagggcctaa agcaggagca
     1741 agtccaataa cgatccctga catccccatt gctgatcctc ttttttctgg cgggaaaata
     1801 gatagcatga tattttgcaa taatggcatc gatactccca cgccgcaagc ttggatcaag
     1861 cgaccgatta gtaatgtact aaaattaggt gctgtaaagc aagtaatcgt tccgatcaaa
     1921 aagatcgtca tggcagaaag atataaattt cttgaactga atttattgat cagccatgct
     1981 gttattggga tcatgatccc gttgactagc aagaaaccag tcgttaacca ttgcacatca
     2041 gacgctgaaa tatcgaaagc tttcatcaat gtaggaaaag ctgtcgtcaa taaggtctga
     2101 ttcaaaacgg tacaaaatgt accaattaat aagacaacaa ctaaaagtgt ccgattatat
     2161 ggttttccgt aaatatctac gggttggttg ttactcatat ttctccttct ttctgaattt
     2221 tttctgaaac tactagaata ctttagtcct aaattcgcta tttgtcaacc tacttgacac
     2281 ataagttaat acggatataa ttaagggaag caagaggaca aaaggagtga caaaaatggt
     2341 tggggcttcc cttaaacgtt tgttggaaaa tgatcaattg gttgttggca tcagtgaatt
     2401 aagtgatatc gtaggtgtgt ctccacgtca attaaggtat tgggaacaaa agggattcat
     2461 tcgttcgatc gaagctgatg cgaattgtcc tagaaaatat cgtttaccta cagtcataaa
     2521 agtagaactg atcaaaaaat atttagatga cggatttact ttgtcaaaag ctgcagaaaa
     2581 ggcagaaatc cgccaaaaga aaatgcacca tgtccgtaaa gttttttcaa aagcaatcaa
     2641 agatatcgaa ttgatcgacg aacgctatac gattttacaa atcggtaact tcaaagaaga
     2701 gaatgctaag atgtacatca ttcatgacaa cgagacagac gaattaactt acaagctcct
     2761 tcctgtcgat gaaaaagctg actacgaaca gttgttaaaa gaactctaaa cgccactaat
     2821 ctcctaagta ccaagctttt taataagtag atgctacata aatacttgaa aaaaggcctg
     2881 tttgaacata agaatatccg aacggtgatc tgcgtttctt tcatatagaa aggaattctc
     2941 catcactgtt cggatatttt tttatttttt tcggttactt tcttctaata catctgttag
     3001 ccaatttgca tattctgttt cccgattgat cgcatgcgct aaaatcaggt agtgtccgta
     3061 attttcatgt ttttcttttt cagtaggaaa gatcgttttt tttcgctcta atagatgaag
     3121 tcgtttttct tcatgaaggt cacgctgctg ctgaataatt tctactaatg ccggatcatt
     3181 cacgtctttg acgaaataca atttcagaac aaattcgtct cgattaaccg gtagttcatt
     3241 ggttggtgta tgaatccact ctgccaattg ttccttccct tcttctgtta attcataaac
     3301 tttcttttcc aatttttcac ctgtgatctc tacttgatgc tgaatgattc cttgctcttc
     3361 catttttgca agttcaggat aaatctgact atgttttgct tgccaaaatt ctcctatttc
     3421 atttttgaac gattgattca attcatatcc tgtcatcttc ttcttactta ataaccctaa
     3481 caaaatatat cgaagtgtat tttttcttgc catcagtttt ccctccttga caaatcttta
     3541 aatccattct atcataaatg agtaatcgaa aacatgtagt ttattacatg ttttaattta
     3601 taaggagatg cttaaacatg aaagaattta aagaattgga agattttatt ggaactcact
     3661 ttatctatac gtatgacaat ggttgggaat atgaatggta tgccaaaaat gaccatactg
     3721 ttgattaccg tatccatggc ggaatggtca aaggccgttg ggtaaaagac caagaagctg
     3781 atatcgttaa attgacggac ggcgtattca aaatcacttg gacagagcca acagggacag
     3841 acgtggcatt ggactttatg ccaaatgaaa agaaacttca cggaatgatc tttttcccta
     3901 aatgggttga agaacatcca gaaatcactg ttactttcca aaacgaacat atcgcagaaa
     3961 tggaagcagc tcgtgaaaaa tatgaaactt atccaaaatt acttgttcct gaatttgcta
     4021 cgatcactta tatgggtgat gcaggtatag acaatgaaga tgttattagc gaagaaccat
     4081 acgatggaat gccagatgat atccgtagcg gaaaatactt cgatgaaaat tataaacgag
     4141 taaaataagg ttgtgaaaga gaggttgtga aatcagcgtt ctgacctgag tattagagaa
     4201 agaaacggaa aaatagttcc tcatgttttg ccgttttttg agctaatacc gagggtcagc
     4261 tgattgaaca ccgtttatca agaggttgtg aaatcagcgt tatgctttga gtaataagaa
     4321 cgctagttag aaaatagctt ttcatatttt ctaggctagt gggcttatta ccgagaagca
     4381 gccgtttgaa caccgtttat caagaggttg tgaaatcagc gttttgacct gagtattaga
     4441 gaaacaaatg gaaaaatagt tcctcatatt ttgccgtttt ttgagctaat accgagggtc
     4501 agctgattga acaccgttta tcaaagattg taacaactgc atattcacac agttaaatta
     4561 gtgacttcaa aatgaaataa tgctcccagc aaaaaattag gctgggagca ttatttcgtt
     4621 attctgttca tcaaaaatca gcgttttgat tccatttcat cacaagttcc tcctcattta
     4681 ctgcttcatc tttttgctgt gtttgaattg taaaaccttg tgaaagatag aaatgaatag
     4741 ctgattgatt tttttgatag actgtcaaag tcagtgtttt attggtttct tttatttttt
     4801 tcattagttg cgtaccgatt ctttgttgcc ggtattccct tttcacaaag attcctgcga
     4861 taaactgatc aatcacccca gcaaaaccaa taatttcttg atctatttct gctaagtaaa
     4921 tcactgcatc tggtaaagct ttcctaacag attctacttg attctcccaa taagttgatg
     4981 gaatgaattc atgagaatcc aagttagcat gcagccaaat cgatagaaca gattccagtt
     5041 ctttggcaga caattttgtt gttgtgcgga tcattttttc tctccttact tattcattga
     5101 ttttaaaata aaaaccattt ttttgaagct cttttacaag ttttcgcccc tcaaaaaagt
     5161 atatcttctc ttgttttact tttctaatac caaaagaaac agcaacttta ggatcatcag
     5221 ctgaaacgat tcgataagtt ccaccaggca attgatccag cttgactttc ccatgatggt
     5281 cgctttgtac tgaaaacggc acaccatttc tagtaagtgt ttttttctta tgtttcaaaa
     5341 tgaatgaact accatttacc ggtttccctg acgaatcact aataataaat gaaaaatcaa
     5401 gcactttttt acttaatcta tactgataga ttagtcgtcc gataaacact aacagtatga
     5461 ctacagctat tatcgttcct gcaagaataa accattgccg catggttctt tcctgatttc
     5521 cttttccttc ttcttttttt accgattcgt tataaggaat ccgatgtcct gtcactagca
     5581 aacgatgcgt gttgatcata tacggcgtac atgtaagcaa ggtaactaag tcttctcctt
     5641 ctgaaatcgt tagaacagac gtgtcatctg gcagtaccac ttgtatattt ttaacttgat
     5701 aagccaattt ctcccccaaa acagtcaaaa taaacgtgtc tccgttttct aaacgatcta
     5761 attcggtaaa taaccgtcga ttaggtaaac cgctgtgagc agagatcact gaatgcgtcg
     5821 aatttccacc aagcgggaaa gaggttcctt gcaagacgcc tgctccattt tcaaggatcc
     5881 taccattgag ccgatcataa agtgaaatat ttaattggat cttaggtata ttgatacttc
     5941 cgatcagcca ttcctctaat tggctggatg ttgtactttc atttctgttc ttttcattga
     6001 aaggatcagt accaggattg aatccttggt cagttaatcg ttgttttttc tcctccattt
     6061 tctttcgctg cacttcaatt tcagctgcgg tccgtttttg tacttgttcg atacgttttt
     6121 gatcaataaa actatttaaa gaatccacat aaaatggata taaagcgatc agtaacccta
     6181 cgacgataca tattaataaa aacaaacgaa acaaaagtcg acgagactgt ttttgcttac
     6241 tctgcttcac atgtgtcctc ccttgcaagt ttttttgttc tttcttttat tatatgggtt
     6301 caggaaaaaa atagaaaatg aaagccgatg aggttgtgaa aaagctgtcc tgcatcaagt
     6361 aataagtaca gcccaaaaaa atagctactc atattttttg ttgggttggg cttattaccg
     6421 cagatgcaag cttttgaaca ccgtttaagg aagaggttgt gaaaactgcg ttatgctttg
     6481 agtaataaga gcgctagtca gaaaacagct tctcatattt tctaggctag tgagcttatt
     6541 accgagaagc agctgattga acaccgttta ccgagaggtc atgaaaaagc tactttgttt
     6601 cgagtattaa gaaaaattct gtttcaaaac aaggagatgc aacaaaactt atgtcgcatc
     6661 ctctctcctt agaactttca cttatgtttt tcttgtaaaa ttcataaata ttttcatgaa
     6721 ttttctaatt tcttgcctac ctctcgcata ggatatatga tgaatttatc taaagtttgc
     6781 aaaggagttc gacaattgaa aaaatatggc ccatttttag caattttttt cattgctttg
     6841 gcatttctca tgcctcattt attcacacat aaactaattt tgggtgctga tgccccattc
     6901 cattacaatc ggttctatga tgccgcgcaa caaattaaat atggggattt ccattatttt
     6961 ccttcccttt atggattcca gcaaagtggc aggatcgtga atgccgttta cggcccattt
     7021 ttcgcttatt tacaaggatt gatcgtgttg cttgcaggta gctggtttca ttatcagatt
     7081 ctttcaaatt tcattcttta tcttatttca ggattttcta tgctgatttt actgagatat
     7141 gtcaatgttc gaaattgggt aagtgtacca gtagccgttt tattcatgag tacgtattct
     7201 attcaatact ggtctatcaa ccaaggattc actagctggg ggacttgttt ttatccttta
     7261 tgtatgatcc ctattgctga tttcgtcctt cataaaaagt tccctgttat caaagtcgct
     7321 gtctcagtgg gacttatgac acagatccat ttactgtctt cgatcatgtt agctttgatg
     7381 tatatccctt tttatcttta ttactttttt aaccagactg aaaaaggaaa agcactgctt
     7441 caactgatta agtcggtctt gctttatttt gttctgacag caaatatttg ggcaggatta
     7501 gctattgttt acagtgggaa taagattctt cctccctttg ttaacagaga aatgagtcaa
     7561 aaggcgatag atctggatgg cagttattgg ctccattacc ctaaggcctt tcctatattg
     7621 atattatcag gttttttgct ttttattttc tttcaacaaa agaacacctt attccaaaaa
     7681 atcgtgttgt acacaagtct tgttttcttt tttctttcta ctagcttgat tccatggacg
     7741 acattgatcg aaaaaaatgt gccttttatt agtttgatcc agtttccatt tcgatttttc
     7801 gtgccattta cgctattgct tctactttac cttgcccttt cactgaacca gtggtctgaa
     7861 caaaagtggt tccgtctttt aagtatattt ggcattctta ttttttcagc acagacgatc
     7921 cagaacttat accagcatct ggaaaagtgg gaaaatgaaa catttgtcag tcgacatact
     7981 tatctattcg atacgccgga agaagcaagg aaaacattct tcagcaaaga tatgtctacc
     8041 agcttgttcg ttttccaaaa aaccacacct gactatctgc caatctataa ggagacaaaa
     8101 gacaataaat acgaccggta taacgaagaa atactagaaa acgaaacaac ttttataaaa
     8161 acacatccag gcggtctttt gacaatcaag tggaaaaaca ctggggacaa aactgttcat
     8221 atccctgtta tcgtctacga tcggactgtg ctacagcaaa atggaaaaac attgactgat
     8281 tatgaagtaa cagatatcgg gaccccaatc gtgaagcaga aaaaaggaat caacgagctc
     8341 acgcttcatt atcaaacgcc aatctatttc tatttcattc tttctcttac tttgattggc
     8401 tggtttacct tgctatgttt atttatttat catagataca agcttttacg tgcttaggca
     8461 aaagggataa atacatccga tcaaaaaact ctccaaacgg tataatcgtt tggagagttt
     8521 tattttaatc agttttttta tgttatatgt tatatttttt attcgttcac atctaattcg
     8581 atttccaccc cgtagtgatc actgatgatt ggactatcga tcccatcaaa aatccgttga
     8641 tattttttaa cctgcattcc ttcagggacg aaaataaaat ctatacgcaa tttttctgta
     8701 ttttgttccc aaccatctat tttcttttcc acagtagctg cttttccttt ttcattcgcg
     8761 acaacatacg aatcttgaag aagaaggcaa ctttctctta cagtataata accttgagag
     8821 tctactggat tattgaaatc acctaggaaa aaaagtggta cgcgagtttc caaaaagtag
     8881 ttttccaagt ttttccactc aaaataaaag ccactttttt tatctgtcca ccaagaaaag
     8941 tggcagctcg agacaaaaat gtcctgatca tctatctttg ttttcccaat caagatcttg
     9001 cgtgtctgat aatcttcttt cttttgagat tgggagacgg taaggacctg actttccaat
     9061 gtttttttcg taagcagtgc atttccttct tcatatttat cataaccgat gtggctcatt
     9121 tcccaactcc aaaaatactc ttttccgtgt tcctttaaaa gctgaactaa acggtaggca
     9181 aaattatctt tacggatagg tacttgattc tttacctgac agaacttcat tagcttcgca
     9241 ggttctactt cttcagattc cagtaattgg ttgatttctt gtaaagcaat cacttcataa
     9301 tcttctaaca ggatcttatc tgctaattct tgcaattttt tctctggttc tggctcaagc
     9361 cagctatgcg tgttgagtgt catgaatttc attggttttc tccttgtctt catttcgaaa
     9421 caaaagggtt gtgacaaaat ttttgtcaca acctctccag caaatagtgt tctcctgcta
     9481 actcctcagt aataagctca atggaccagg agctattttt tgtttggctg tttttatcac
     9541 ttggtgcagg gcagcttttt cacgacctct ccactaaaca gtattcaaac tgctgcttct
     9601 cggtaataag cccaaccaaa cgaaaaatag gaggagctat ttttgtttgg ctgttcttat
     9661 gacttgatgc aggacagctt tttcacaacc tctccttaca ttttaatgga gccgatcact
     9721 ttaccagctg tcactgtacc taattgcttg atcactactt cttctacttt atcgtttgta
     9781 aatacgatca tgatcgttgg atctttttct tcgttttgga tcacatcaag atccatctca
     9841 gctaatttcg ttcctgttcc tacttcttgg ccttctttga cagcaatctc aaaagctggc
     9901 tgattcattt ggactgtatc gattcccata tggatcaaaa cttcgatacc atctttcgtt
     9961 tccagcccaa ttgcatgttt gctagggaaa atacttgtca cttttccagc gatcggcgca
    10021 acaacttctc ttgatgttgg aataacagca aagccgtcac ccatcatttt ttgtgagaat
    10081 accggatctt ttacttcaga taaaggaatc acttctccag aagctgcgga aactagttct
    10141 tgttctatac ctaagtattg cttactacct gcttgtccat ttcctgtaga aaccatttct
    10201 ggctctggaa tctctgctcc tgattgcagc agatcttcga tatctgattt caagacatct
    10261 gcttttggtc cataaactgc ttggactcca ttgtctttga cgatcaatcc catcgcccct
    10321 gctcgtttcc aggcttcttc agtccctact ttttctcgat catttacact tacgcgtaac
    10381 cgagtcatac aagcatcgac atctttgata ttttcttttc ctcccagcaa atgaatgatc
    10441 tggaccactt gctgatctgc tgttcctgtt ccgctgttag atacaacggt ttcttcactg
    10501 tcattgtcgt aatttccgtt acgtcccggt gtagcaaagt tgaatttttt gatcaagaaa
    10561 tttgctaaga aatacgtaac aacaccaaaa gcgatcacac acaacacgaa gttgataaga
    10621 tctccaccta gacccgcttt gatagctaac ggtgtccgag tcagcaattc gatattccca
    10681 aatgaatgga cccgcaatgg caagatatct gccatcgcaa atgcagcccc ttggatcact
    10741 gaataaatga catataatgg cacagcagca aacataaaca tgaactccaa tggttctgtt
    10801 acacctgtta gaaagacagc caacgctgct gaaaaataca ttgatttata tcttgctttc
    10861 ttatcaggat cgacattacg atacatcgca aaagccattc ccatcaagat acctgaagaa
    10921 ccaatcattt gtcctacttt gaaacgagcc ggcgtccagt tatgcaacac aaactcatat
    10981 tgcgacatat cccctgcacc tttaagattc actaaatcag tggcccaagc caaccataac
    11041 ggatcttgcc caaatacttg tgtccccgcc tgtgcgcctg ataaaatctc atacgatccg
    11101 ccaagctgtg tatagttgat aggaatcgtc agcatatgat gcaatccaaa tggcaatagt
    11161 aaacgttcta atgttccaaa taaaaacggt gctagaatcg gtgcgctttc ttgtgattct
    11221 gcaatccata aaccaaaatt attgattcca gcctgtacgt ttggccacac cacagcaaga
    11281 atcaatgcca cgattgttga ccataagata acaacaaacg gaacaaagcg ctttccattg
    11341 aaaaatgaca aagcatccgg caattttcta taattatagt acttattata agccattgcc
    11401 ccgacaaatc cagcgatgat ccctacgaat actcccatat tcaaagctgg tgcttccaat
    11461 acacttgtaa aaaatccttt aaccatgatc ttcgtaccga acagagtgtg ggtaaatgca
    11521 tcttcattcg caagcatgtc acttgtgaca ccaaagatag ccccagtgat ccgattgatt
    11581 aaaataaatg agatcccagc tgcaaatgct ccgcctgctc tttcttttgc ccaacttcct
    11641 cctattgcta aagcaaataa aatatgaaga ttaccaataa ttgcccaacc aatattttca
    11701 attactccgc ccgttgtgac aagcggcgtc caatcaggat tgatcaaggg aattgatttc
    11761 cctatactga tcatcaaccc agctgccggc ataactgcta ccacaaccat taaagccttt
    11821 ccaaattttt gccaaaactc aaaactaaat atctttttca ttccgtttag ctcctttacg
    11881 caatcggttt cgttaaacgt attatagcgt gcgttttctt ttttgtaaat gctttatttt
    11941 ttatttttct gtctatacca ttctataaaa cctataatac gtgtgttccc ttctataatt
    12001 ttctcctaaa aatttatttt tttaagcttt tttctatgaa atattttctt ttgtctattg
    12061 acttgtgcaa tcggttgcgt ttattatgaa cgtataaaac agctcattag gaggagaaaa
    12121 atgaaacaga tcaaacgact atttgctatt gatccatgga aaatcagcac gacacaatta
    12181 gataaaaaaa atcttcgact gcaagaatct ttaacaagta ttggaaacgg ttatatgggg
    12241 atgcgcggga actttgaaga aacttactca ggggaccacc atcaaggtac ttatttggcg
    12301 ggtgtctggt atcccgataa gacacgtgtc ggctggtgga aaaatggtta tccagaatac
    12361 tttggaaaag tgatcaacgc cttgaacttt atcgcgatag atatttcgat caacgatcag
    12421 ctgatcgact tggcttcttt ggaaccagaa gacttttact gggaattaga tatgaaaaat
    12481 ggtgtattat ctcgtacatt cacaatcact acttctacta ataaagtacg catttccttc
    12541 gaacgttttc tgagcattgt caaaaaagaa tcagcttata tctatttaac tatagaaatg
    12601 cttgaaggta gcggaacagt caaactcgta tcaaaattgg atggaaatgt tcaaaacgaa
    12661 gacagcaact atgatgaaca tttttggaat gagattgatc gtggaactat agaagaaatc
    12721 ggctatttga cgactaaaac tattccaaac aactttggga ttgaacaatt tactgttact
    12781 gcagcaatgc gccatttcgt taatggaaaa tcagtcatcc ctgactatca cgaagatact
    12841 ttagcattac atggttgctt gtctgcagaa ttatctcaag gtgagacatg gtcagtggaa
    12901 aaagaaatta tcgttcttac tagtagagat attccagaag cgcagcaagc agaaaaagct
    12961 gtctcagaat taacgaacca gacaaacaat tatatccaag caaaagaaga gcaaacagcg
    13021 gcttgggcag aacgatggaa gcttgccgat gtggtcatcg aaggagacga agaagcacaa
    13081 caaggtattc gattcaattt gttccaacta ttctctactt actacggcga agatgaacga
    13141 ttgaatatcg gccccaaagg attcacagga gaaaaatacg gcggagccac ttactgggat
    13201 acagaagcgt atgctgtccc tctttatctt gctttagcca aacctgaagt aactaagaat
    13261 ctgctgaaat atcggcataa tcaactgccg caagcaatcc acaacgctca gcaacaagga
    13321 ttaaaaggcg cgctttatcc tatggtcacc ttcactggtg tcgaatgcca taacgagtgg
    13381 gaaatcacat tcgaggagat tcaccgaaat ggcgcgattg cctatgcaat ctacaattat
    13441 acgaattaca ccggcgatac ggaatatttg aaaaatgaag gactggaagt tttagcagaa
    13501 atcgcacgct tctgggcaga ccgtgtccat ttttcaaaac gagcaaataa atacatgatc
    13561 cacggtgtga ccggcccaaa cgaatatgag aacaatatca ataacaattg gtatacaaat
    13621 acgatcgcag cttgggtctt gcgttacacg cgagaaaatt atttaactta tcaaaaagaa
    13681 gctgctatca ctatcagcga agaagaacta gccaaatgga aagatatcgc agagaacatg
    13741 tacttcccag ttgacgaaga attaggaatc tttgtccagc atgacacatt cttagataaa
    13801 gatctcatgc ctgtatccga tattcccaca agcgatcttc ctctgaatca gcattggtct
    13861 tgggacaaaa tccttagatc ttgcttcatc aagcaggcag atgtactaca ggggatttat
    13921 ttcttcaatg atacttttac aaaagaagaa aagcaaaaaa actttgattt ctatgaacca
    13981 atgactgtcc atgagtcttc cctttcgcca agtatccatt cgattttagc agcagaatta
    14041 ggaatggaaa gcaaagctgt agaaatgtac cagcgaacag ctcgcttgga tttggataat
    14101 tacaataacg acacagaaga cggcttgcac atcacttcca tgactggtag ctggttgtcg
    14161 attgtccaag gatttgctca aatgaaaact gcccatgatc agttgagatt cgcaccattt
    14221 ctgccaaaaa cttggaaagc ttattctttc catatcaatt atcgcggacg tttattgttc
    14281 atctcagtca cagatgaaca agttactttc gagttattag aaggttttcc tctagaaatt
    14341 tacgtctatg aacagtctta ttctttagcg gatcgtctag aaataacgct aagaaaggag
    14401 gaagcaaatg tttaaagggg tattgtttga tttagacggt gtcattaccg atactgctga
    14461 attccattat cgtgcttggc gaaagcttgg tcaggaaatc ggcattacga tcgaccacac
    14521 attcaacgaa cagctaaaag gcgtcagccg agaagactca ctctctttgc ttttagctta
    14581 tggcggaaaa gaacactctt tttcaaagga agaatttgca gaattagcga aaagaaaaaa
    14641 cgattattat ttagagatga tccagacgat cgaaccaaaa gatgtcttcc ccggggtcgt
    14701 gcctttactg gattctttaa aagaagaaaa aatcaagatt gctcttgctt ctgcaagtaa
    14761 aaacgggcca ttcctacttg aaaaaatggg tcttacacct tactttgatg ctattgccga
    14821 cccggcagaa gcaacaaacg gaaaacctgc gccagatatc ttcctccttg cagcaaaagc
    14881 tgtcggacta aaagcagaag aatgtatcgg gatcgaagac gcgcaagccg gtatccaagc
    14941 catcctctca agtggcgctc aaccaattgg cgtgggacgt gctgaagacc ttggagagga
    15001 gatcccgctt gtccctgata cgacttttct tacgatcgat tacttgaaag aaaagtgtca
    15061 tgaccatgga taatccagag atctccgtcg taccagttcc agagactgaa ataaagaaaa
    15121 tcacattgaa gaacgactac ttagaagtcg ttcttctcaa ttacggtgcc cgcctgcacc
    15181 aaatcttcgc accagacaaa gaaggaaaat cagaaaatgt tttattgtct tacgatcgtt
    15241 tcgaagatgt tctaacagac aaaagctttt ttggagcaac tgtcggacct gtagccggaa
    15301 ggatccggga tgcttcttgg ggtgagcata gattggagaa aaactgcgga tcacaccata
    15361 tccacggtgg cacgaaaggc tggtctttcc aattttggga agtagaagtt ttcaaaactc
    15421 catcatcaat aggtgtggtc ttttacttaa aagatgaatt ttctacctat ccaggaccaa
    15481 tcactgctac gatcacttac cgtttaacaa aaaacgaatt agagatgaca acctctgcta
    15541 gtagccttgt tgaaaccatc tgcaatccga ccaaccatgc ctacttcaac ctttcaggaa
    15601 atggaacaag agacatctat gaacatcagt taacggtctt tttagatggt gtgcttgaat
    15661 tagatcaaga aaaactgcct actggaaatt ggacaaaaaa agaaaaactg ccaatcgact
    15721 ttagaaaatc cccaacactg caagagatcc ttgcatgtta tccagatgga ctagatgatg
    15781 tatttctttt gcatcatcct aggctttcca aaacttcctt acaattattc gagaagcatt
    15841 ctggtcgcca aatgactata gcaacttcta ataaaagtat ggtattattt tctacgactg
    15901 gattcgaagc tgatttctct gtcaatggaa aacagatgca ttcaaactac ggattagcaa
    15961 tcgaaccgca agaaatccct gatatcgttc atttccccaa atttggatcg atcaacctcc
    16021 atcctggtca agaacggatc agtcagacga tttatcggtt ctctgcacaa taacaagaga
    16081 aaggtgtttt tatcatggct attaccgtaa aagatgtggc gaaaaaagcc ggtgttgcca
    16141 cctcgaccgt ctctcgtgtc atcaacgatc atcctagtat ttccgagaca acaaagaaga
    16201 aagtccgaaa aattatggat gaactaggtt atgttcctaa catgactgcc cgcaatctag
    16261 gaaaacgcat ttccagtgcc atcggcgtaa tcttaccccc tcttgattca aaagagcgga
    16321 tcggtaaccc attttatctt gagatcatgg aagcaatcaa cgaagaagcg cggaattatg
    16381 atatgactac agcaatcgct actgctaaaa attttgaatt gctgttagag aacgtccagc
    16441 gtatgcattt acaaaaacaa gtagatggtt ttattctagt gtattcagat agtcaagatc
    16501 ctgtcattga ttatttatac caaaaccaca tccctttcac tttgatcgga cagccttatc
    16561 atcatgagaa cgaaatcgtt tatgtagata atgataacca gcttctaggt aaacaagcaa
    16621 cagaattttt gatccagaat ggacataaga atatcctgtt tgtgacaaac acgactcatg
    16681 aaagtttgta tttcgaacga tatttcggtt atcaaaaagc aatgatgatg gctggtcttc
    16741 ctgcgttatc cagtgtagta ctagagcatc cggaggatta tctgtccttt gagaaaacgt
    16801 tgaaagaaaa cgaagccaca gcattggtgg tcattgacga tatcttcgcc cttcgcacga
    16861 tgcagttggc gcaaatgtac ggctatgtca ttccagagac actctccgtc attagcttca
    16921 acaactcgat cttctcaaca ttaacacacc cttaccttac aagtatcgat atcgatatct
    16981 ccgaattagg acgtgtcgct atgcaaaagc tgcgcgaatt gatccatgaa gaattgagca
    17041 gtggcgtacg tttggtcatc cctcatcgct tgatcaaaag agagacagtc gttcctttag
    17101 aaaacccata ctaaatagct gactcactaa atacgctgac catttatcct atggtcagct
    17161 ttttatgtaa aagaagatac tacttctaac acattgttta tctgtaaaaa agagcgtggg
    17221 acaaaagtaa agaacacttt tgtctcacgc tctaaacacg tataaacggc ggaagcagaa
    17281 gcaactcctt cggaaataag ctgaaattca caaaaattta aaaagcaatt ttcttgagtt
    17341 tcttcttatt tcttggagtt aaacgcttct gtcccagcct cttttttcct tccttcaatt
    17401 ttttacacgt cgacgattca ataaaaaacc gcctaatatt gtaaagacag gtgcaagaat
    17461 cgagaaaaag acgaatggca agtaatgcaa cgcgctaact ttcatcgttc ccataatgaa
    17521 tgtgccgctg actccccacg gtacgagaga attgacggtc gcacctgaat ccgctagtgt
    17581 tcgtgtcaaa taggtttttg ataagccaac gtcatcaaaa gaacgcttga atgtttctcc
    17641 tggaagaatg atggacagat actgttctcc aacgatcaga ttgattccga tacagctcaa
    17701 ggcagtaaac ccgactaaac gagaaggtcg gtccatcttt tcttttaatt cttgaacgat
    17761 ggtatcaacg atcagatatt tgatcagtaa cccacctaat gccaatgcaa gtaaaatcaa
    17821 actagcagaa cctaacatac tcatgatccc gcctcgagaa agcagttcat ctagctttgg
    17881 atcaggtgta tttgcgacat aaccattcat caagatgtct gctgcttttc cgaccgtcaa
    17941 acttttctca tggataaaag aaagaacgag agcagctgtc gatcccgcta gtaatgtagg
    18001 gatagccgga atttttttga ttgccccccc aaataaaata actagtggaa tcaatgtcca
    18061 aggagagatc caaaagctcg ctttcatgtt ttggatcatt tcattgatac tagctagatc
    18121 ggctgaatga ctgttatgtc ctaatatcgt ataaaaaatc aacgaaagga cgaatgctgg
    18181 gaaatccgta taacgcatac cagcgatatg ttcgaataga ttaactcctc caatacccgc
    18241 tgccaagttg gtcgtatctg ataaaggcga gcagttattc cctaaaaagg caccggaaac
    18301 gactgcgcct gtagtcattg catcattgaa tccaagtaaa tgaccgatcc ccaaaaaagc
    18361 aattccaatc gtcgaaatcg ttgtaaatga actgccgaca gtcactccga caattgcaca
    18421 aacaagaaat gctgtcggta aaaagaaacg cacagaaaca agtttaaatc cataaaccat
    18481 aatcgtaggg atggttccag ataaaatcca tgaagcaact aagacaccaa tcaaaagaaa
    18541 aatcaggatt ggaatgatcc ctggagtgat tcctttgatg atcccgtcat ggatctcatc
    18601 ccatgtgaac tttttaaatc tgccataaaa caacaaacac atgaaagctg caaggatcgg
    18661 gatatgtggc gagagctgga atccgatgat cagtactcct aggagtgcca acaacacgat
    18721 caatacagcg atactttctt ttaaagaaat atttttattc atcgtcgatc tcctatttca
    18781 aatgaaaagg ccctgccatg aaaaaactat tcacttggca ggacctccta ctaaatcagc
    18841 gacaaaacgt cggctgaaag acttattttt tttatttttc ttctaaactt ttttctacct
    18901 caatccccca agaatctttc ttgaagtgat tgatgatcaa tggaataacg aacatgacca
    18961 ctgtgatagc tgctactaat aatgcatcac tcaatgtcgc tgtacctaat gtagcaaata
    19021 cgacaacgat gatagagaag atcagcatgc agctgactac ccaagctaat ccgtttccgt
    19081 ttttaccaat tcggtaagga cgttcgaatt ctggatgttt cttacgcatt ttaagcaatg
    19141 aaacagcaat caaaatgtac acgatacaat aaataacagt tgtagcattt gtaagtgtca
    19201 agaatacagc acttacgtca tccattacac cataaagcaa agcaaataat gaaataacga
    19261 ttgattgtgt tagaacgaca tttctagata cgccgtattt gttttcttta tggaatccga
    19321 attttggtgg aaggaatcct tcacgagcta cttggatgat tgtttttgat ggtccagtaa
    19381 cccaagctga taattgtaaa agcacaccaa taaataccat gaagctgaag atatttccga
    19441 tcacttctgg taagcctagg atttgacagt caatcaagat aggctgcgtg atatttgcta
    19501 attccatttt tccatctggt acgacatttg ctacaagcat cgcattgatg acattcaata
    19561 aaactaatcc gatcaaagcg ataaatacgc cttttgtata attttttgtt gcgtctttca
    19621 aacgaggcat gtaaacagaa ctcatttcga tcccaacaaa aataaatgtg ataccagcta
    19681 agtatttcaa tgaatcaata tgttccaagt cagggaaagc ttttgaccat gaaaagtcac
    19741 ctagatagcc gtttgagact agtccgactt tgaaggctgc caaaacacct aaaacgaaca
    19801 tgatgactac tgggatatat acccctagcc atacaccgat gttcccgccg acttttgcca
    19861 tatcgaattt caagttcaag atcgtgatga tccagtagat aactaaaata caacctaaaa
    19921 cgaaccagtg attattgcca agttctacat taccaaatgt gtttcctaac aatggtccta
    19981 aagtagaagc gaccattacc atacctggga acatttgcac ccataatagc catgcaacga
    20041 caaatcccca tttgctgccc agtgctgttt ttacccataa ctgaggaccg ccttcttgag
    20101 gaagcatcgt ggaaagctca ccggaaacca ttgatatcgg accggcaaaa aacaaaactg
    20161 caaaaattga ataaaaaatc aatgtccaac cgactgcggc taacgtagga atactccgaa
    20221 ccgttgcaca cagtgccatt gtcagaccga taaaggtccc aagacccatt ttacttactt
    20281 cagatttact caccgttgac actctccttg attttttgga taaaataacg gtgtctttga
    20341 agtagtttca gcaataaacg aaacaattca gcgaatattt gaggggattc tttgagtcta
    20401 ttgcttgaag ggtttcttca aagacaacct caaaacaaag ggatcgaaga gcttaggtct
    20461 ccccttgctc ttcagatcca aacatgcgtc agaaacagtt agtattcaag ctgttttcta
    20521 acatgattac cttgcttatt ttgcttcgct tgccaataat tggtctgcgt agattttttc
    20581 caatttttct tgaagagctg ctttgatttt ttcagcatat tcttcaaagt gttcttcttt
    20641 gttcatgtat ggtgtcatta cgctggcacg caatacagtt acttttccgg cacggttcca
    20701 ttcttcatct gagaatccta gttggttcac aaattgtaat gggctgttgc catagtctgg
    20761 gatagcaaag tcagtatgtg aagttaagaa ctcattaccg taaatgcttc ctttcacgta
    20821 tgaagaataa tcatatacgt catggttcaa tttattcatt gcaactaagt catcgttgcc
    20881 tttttctttg aatacatagt ctaccatatt gaaatctgga taagttaatg gatgaacttc
    20941 gatttctttg tctcctactt taaatgacaa gtcattcaag aagttgtaga aacgatgtgc
    21001 accttcgatt gatgcaccca ttaatttacc atatcctgtg acattcaaag gtaagacatg
    21061 gtgtgctgcc caaacgctgg ctgctgttgc ccctgctttt gaaccttcca agatataagc
    21121 cccaagtaat gctgggatat ctgcgccttt ttcaaataca tatgtcgcaa agtaagagat
    21181 aacgtctctc atgcggatat ctttgatgac gataccaccg gcagaatatg gaacatagcc
    21241 cattttatgt gggtcaatcg ttacagattc tgcttcttcg attgctttat atgcactgtg
    21301 tacttcttct aagatataat ctttgttttc tgtaaagaca ttgtatttgt aatgaacatc
    21361 ttttaaatct tcaaatggaa tgaagttgtt gtcttcgtct aagaaaatcg cacggccgta
    21421 tccgccataa gcagcatcta cgtgtaagta gaagtaaata ccgtcttttt ctaatacgcg
    21481 acgcaatgca acgattttat cgataccgtc gattgctcct tcttctgttg atcctacaac
    21541 accgactaca cctaggattg gtgttttttc agctgctaga ccacgaacga ttttttcaag
    21601 ttcgttgatg tccatgcgat agttgtggtc aactggcact ggaatcactt ggtctaaacc
    21661 gataccgatg atgtcggcag cttttagcca tgaatagtgt ttagtttgtg gaaccaacca
    21721 ttttcctaat ttttctaaat gttttccgct acgtgctgag tgagctttga tttcgtcgat
    21781 tttttctggg acgctgtcta acagattcat gatttcttct gttgacaagt tcattaattc
    21841 ccaatcgctt tttccagcaa ctagttcagg tgtcacttct ttcattgcaa gcggcaatga
    21901 tttgatgttg cgggcatacc aaagtccttc taagtttgct aaagaaccat cagcaacgat
    21961 gtggccccag ccatctttat agctcattaa ttttgcaaat tccattccta cttcttcttc
    22021 catttggctt gttgcaggag aagattcata ggcaacgtta ttaccattcc atagcatagc
    22081 aaaattgtac gctagtaaag atggcatcaa tgtttcagag ttcatatgac cccagtaacg
    22141 tccggcatta tgccaaggaa cggaatgggt acgcatccgc gctgaaattt ctgatagaac
    22201 atcctttgtt ttattcacag tgtgttcaaa actagcactg cttttttctt ctggcgtaat
    22261 aataggcatg tcttgaggca tgtagttttg acgccagcct aaatgttcat ccaccaattc
    22321 attcaacaaa gctttataga tttggccgtt ctcagctttg tccccaatga atagggcatt
    22381 taggtttaga tctttcgaca atgattcact catagtgaaa ccctcctaaa aaaatattta
    22441 aattttgtcg agccctacat tacacctgtt cttctattct gtaaactaag ttttggcctg
    22501 atttttaatc atattttaat atgtatcaat tatccgaatt gttcacactt ttgaaataat
    22561 taaaaaaaac gagggaattt acaaatgtaa actccctcgc aaagattgta ttaacgacaa
    22621 ccagcgttct attttaggat gggtttcaaa attggaatgt tgtaatagta tttccctttt
    22681 tcaaggtaaa aacttactaa attctaatca attactttgg ccaaaaagta atttttctta
    22741 ccttttcgaa taaccacaaa cttaccatcg aattcatcgg atggatttat gacaaaattt
    22801 aaatcggtaa cgcggtcacc gttaatactt atagctccgt ttgaaacatc ttcacgtgct
    22861 tgacgtttag atggttcaat ttttgtggaa acaagcagtt ccacgatatt ttcaggtgtg
    22921 cttgaaattt caacgttcgg cattttaccg aatccttgcg cgatttcttc cgcatttagt
    22981 tctttaatat ttccagagaa caatgcttgc gttatttttt gcgcttcttt caagtcttct
    23041 tcactatgca caaatcttgt aacttcttct gccaaacggc gttgtgcttc acgtttctca
    23101 ggttctgtct ctactttttt cgctaagtct tcgatttctt cttgagacaa gaaagtaaag
    23161 aatttcaagt atttcactac atcgcgatca tcttgattca accagaattg gtagaactca
    23221 aatggtgaag ttttcttcgg atcaagccaa acggcaccgc ctgcagtttt tccgaatttc
    23281 gttccgtcag ccttcagcat caatgggatc gttaaaccga acgcttctac ttctggtcct
    23341 tctaattttc ggattaggtc taatccagct gtgatatttc cccattggtc tgctccaccg
    23401 atttgcaatt gcacatcgta tgttttatgc agatgcaaaa agtcgataga ttgcagaatt
    23461 tggtaagtaa actctgtaaa tgagattcct acttccaaac ggctggcaac gatatctttt
    23521 gctaacatcg tgttgatgtt gaagttttta ccgtagtctc tcaaaaattc aagtaaagag
    23581 atttttgaca accagtcgta gttgttcaca aatgtgatat ttgcatcttt accaaacaat
    23641 tttctcattt gattggaaag tgcgtccacg ttatgttgca cttgttccat cgtttgcaac
    23701 acacgttctg ttttgcggcc gcttgggtcg ccgatcgttc ctgttccgcc gccaatcaga
    23761 atatatggat gatggcctgc caattggaat cttttcatca tcataaatgg aatcaaatgt
    23821 ccgatatgca tcgaatcacc agttggatca acgccgcaat aaagtgaaat gctcttttct
    23881 tcaacgagct cgcgaagtct ttcttcgttt gtttgctgat taatggcgtc gcgccaagtt
    23941 aattcatcaa taatattcat tttactgacc tccgtgtagt ttttttatga aagcaggaac
    24001 ctgctagcat gtgtaattaa ttatttcttc aggataggat aggaaataaa aaaagtccct
    24061 tggcatataa gccaagggac gaaatctcgc ggtaccaccc aatttgtatg tctagatatc
    24121 caacatacct ctcgaacatt taacgctgtc gcgtggcaat cactgccaaa gactcctgaa
    24181 atgtaattcg ctttaaagca tgtactggtt tgcagcgacc accagctttc tgaaacattg
    24241 gctttaacaa ctacttcgtt tcgatcttcg tcttgttatt attaatatgt gtctatcata
    24301 gagcgcaact ttttctttgt caacagaaaa aggatatttt ttttaaattc ctcatttttt
    24361 cttttttgaa tccgctctaa ttccgaacat tttattttca attattgtta aaagaaataa
    24421 ctttttctgt attttttctg caatttatcg agaagtcact ttactttgcc tcttattctt
    24481 tcttcgttac aataaggaat gggacaagta aaaaaatgaa aaagcaaggg acgaggtata
    24541 tggaaaaaaa gcaaaaaatt attttaggag ccacttctgc tttaactatc gttattttaa
    24601 ttctaagttt gttttttgtc atccgatcat ctacaaaaaa agaaccaatc attcctgaaa
    24661 aaaaaataac agaaaaagca aaagaaccga cagatgataa gcaacaacta gttactcaag
    24721 caaaaaatct ggctcaaagc tacgattatg accaagcgat tgcgttattg gaaaaagata
    24781 gtaagaacgg atctactgaa acacaacagc ttcttcaaac actaaaaaag gagaaagaac
    24841 aactgatcga ctggtctgat cctactcaga tctctcatgt ctttttccac agcttgatcg
    24901 tggacccgca aaaagccttc cactctcaac aggcacaagg atacaaagat tatatggtga
    24961 ctgttgaaga attcaaccgt tcgatcgaac aactttatca aaatgatttt gttttagtta
    25021 atttaaatga tttgatccaa aaagacgaac aaggaaatct ttcctttaac gggcttgcgc
    25081 tacctgaagg aaaaaaacca ttggttcttt cacaagatga tgtcagctat tatgaatata
    25141 tggataattc cggttttcct agccgattgg tcgtatcaaa taacaatgaa atcaaaaatg
    25201 tctataaaga gaaagataaa gaagaaatcg gtgactttga catggttcct ctgatcgatg
    25261 cctttgtcaa gaaacatcct gatttttctt atcgaggcgc aaaaggtacc ttggccctga
    25321 caggttacaa cggagtttta ggttatcgaa catcaaaatc tcaatatgga gacaatgaaa
    25381 aaacaaaaaa agagattaaa gaagctaaaa aagtggcgga acagttgaag aaagacggct
    25441 ggagctttgc ttcccataca tggggtcact tgaatatgac tcaagcttca ctagaagatg
    25501 taaaaaaaga caatgaattg tggcagcaag aagttgcacc tatcctagga aaaacaaata
    25561 ttctgattta tccatttggt gcagacatca gcgactggca gccttattca gcagaaaatc
    25621 aaaaatttgc ttacttgaag agtcaaggtt tcgatatttt ctgtaatgtc gatgcttcta
    25681 caccagcttg gggacaatta ggtacggatt tctatcgaaa tgctcggatc aatatcgatg
    25741 gcattcgttt tcaatctgat ttagacggaa aaaatcctat tcttgatcca ttcatcaatg
    25801 tccatgatgt ctatgacaaa gaagcacgcg aatctgcttg attttacgtc atttatgaac
    25861 caaaaaagcc gaactgacat tttatcgtca gtttcggctt ttttatgctt tatttcttct
    25921 tcggtgcgat tcccttgatc aggacttctg tccattctgc tgtatatgga agcagttttg
    25981 cgtatatctc cggcttagaa attccttcta ccattgcttg aaaatagagg atcaagaact
    26041 catccgaata tttcaaatcc acttttcctt ctttccgccc tcgatggaaa agatcaagca
    26101 tgatactgaa actttcttgc gtatacgcat ccatcgcgtc agaaaattct tttttttctt
    26161 tttttgtata gtatgtcatc aaatcgatat aaaattgttt gttcactcga ttgagataac
    26221 ggattttgtt ttcactcatg gcaatcagtg tttcttcaaa tggaagattt tccgcaatca
    26281 acgctttcgc ttcttgccct aaatcattca taaatgcaaa aaatacttca taaatcagcc
    26341 cttccttgct gccaaaatat ttaaaaatcg tcgctttact tacctgagcg ttctcagcta
    26401 ccttatccat tgtcactcca gcggttccta tgtctgtgtt catcaagtca aaagctgctt
    26461 gtaaaatctc tttctttttc tcttgtgctc tcttttcaaa accgttcatt tgtagagcct
    26521 cccgtaattt taaactttta taatatatat agttcaaatt tattctgagg tttattataa
    26581 ctatctattg agaaagtatc aagctagcaa ttccttgttt atgaattcat tgacagaaat
    26641 ttataaaaag aatattatgt actaaaataa taaaaaaagt tttataaaaa ggtataaaac
    26701 ctttttatta aactatttaa tcttgtttag tatatatttt tagaaaaagg agtggggttt
    26761 ttatggcaga aatcgtcaaa gtcacacatt tgaaaaagag ctttggtcgt ttcgaagctt
    26821 taaaagatgt ctctttcacc gtacattcag gtgaagtcgt tggatttatc ggaccaaatg
    26881 gttctggaaa atccacgacg attcggacac ttttagggat tataaaaaaa gacggcggaa
    26941 cggcaacgat ttttgggcaa gatgtatggg aagatagttt atctatccat gaacgtcttt
    27001 cttatgttcc aggcgatatc tctttgtggg gaaatttgac tggaggagcg atcatcgatc
    27061 tcttcatgaa gcttcatgga aaagggagtg ctcaaaaaaa agatgaactg attcaacgtt
    27121 tctctcttga tcctaagaaa aaagcaaaaa actattcaaa aggaaatcgc caaaaagtgg
    27181 gcttgattgc tgctttggct gttgattcgg atctctattt atttgatgag cctacttctg
    27241 gtttagatcc tttgatggaa acgattttcc aagaagaagt tgaaaaattg aaagaacagg
    27301 gcaaatccat cctactttct tctcatattc tcagtgaagt cgagcgacta gcggataaag
    27361 tcgtgatcat acgcgaagga acagtcgtag aagaaggaac actcgatgaa ttgcgtcatt
    27421 tgactcgttc gaccatcact atagaaacag aagcagagaa tagtcactta gcacaattga
    27481 acggtgtcca tgactttgtg caaaaaggtc acctcgctca atttgctgct gataacgaag
    27541 ctttcgatgt gattttagct gaagcgtcca aattaggtgt gaaaaaattt gaatctattc
    27601 caccgactct tgaggattta ttcatgagac attatgaagg atgaggagga agcgaaatga
    27661 ctgataaatt ttcacgctgg tccacccttt tttctcaaaa ccttagccgt gactggaaaa
    27721 agatcctgat atggctagtc ggtgtcactg ctttttctgg tggttttgtc cctgcgtttg
    27781 aagaattgac ttccggacaa ggattagctg gattatacga aaccttgcaa aacccggcaa
    27841 tgattgcaat ggtcggacca actccagctt cgaatgctgc tgagtatact ttaggagcga
    27901 tgtatgcaaa tgaaatgttg cttttttgca gtttgtttgc tgctgtgatc tctgttttac
    27961 acgtggtgag ccaaacgaga aaaaaagaag aacttggtct gattgaactt attcgttcct
    28021 ttcgagtcgg acgtcaagca aactctttag ctgtcatcgc tgaaagtatt ctgatcaatt
    28081 tgcttttagc aatatcgatc agctgtacga tgattttttt ccaagcagat tcgatcactg
    28141 cagaaggtgc ttggcttttt gggctatctg taggtagtat ggggattttg gggagtgccg
    28201 ttgcactgtt tgctgcacaa ctgatgccaa gttcagccag ttcgaatggt ctgtctttag
    28261 cgtttttagg tggactttac cttctacgag caataacaga tgtagctgat tcctcccttt
    28321 cgatgtggaa tcctttaggc tggtcttatt tgacctatcc atttacaact aacaactgga
    28381 ttcctttgct ttatttgttt ttgttcagtc taatcttgat aatcagttca tttgttttag
    28441 aggaacatcg agatttgaac accggctata tccccgaacg tgaaggaaaa ccgtatgcta
    28501 aacgttctct tttatccttg cgtggtttat tattctatct aaataaaggg atgacaatca
    28561 gttggcttat cacttttggt ttgatggggg cggcatacgg ctctatctac gggaatatgc
    28621 aacagttttt agaaagtaac gacttaatga aacagatgtt tatccaatct ggcgtctcaa
    28681 ttgaaaaaaa ctttacaagc acgatcatgg tcgtcttgat ttgtctagct gctattttac
    28741 caattgctag catcaatcgt ttatttacag aagaaaccca cggacgtatg aatcagctgt
    28801 tcgcaacaaa aatttctagg aaccaacttt attggaccaa tatgttacta gctattctat
    28861 ttgctatatt ggcaatcctg attagttctg gtgcactagg gctttctgct ttggcttcga
    28921 tgaaggatgc gaaagacatg gatctattga tgtttcttaa agccgggttc aatttcctgc
    28981 ctgccgtttt agtgtttatc ggaattagtg gctttctatt cggatggctt cctcgaatgg
    29041 gtaaacttgc ttatatctac ttagcttact cctttgcact caattacttt ggcggaattt
    29101 tggatttacc agaatggttt tccaaaacag ctgtccctag ctgggttcca cggatgccaa
    29161 acgattcttt tgatctttcc gtgttcctta cgttatcatg tatcagcctt gcactgttcg
    29221 tccttggttc tattggttat aggcgaagag acttactaga aggataataa aaaagcatgt
    29281 acaatttcat gctagcctcc aaatctgggt caaaaatcca acatttggag gtcatttttt
    29341 tatttacaag tcttctaact gctctttatc aatgatccag acttctcgat tctttctttt
    29401 tacataacca ttctcctcta ataatttaaa ttttctagat aaagtttcgg gggtcgtacc
    29461 gataaatgca gcaagttctt tcattttcat tggcaggaaa aagtgatcct tccctgtcgc
    29521 tttagctaag tcaagcagat agacagcgac tcgttcttct actttttcca tcatcaaaaa
    29581 agttgtctgc tgctgtactt ttgttaattt ctcaacgttg attgctaaaa gtttcagact
    29641 aagggcggga taatgcagta agagttccga gaaatctttt tgtctcaata tgcaaatttc
    29701 cgttttttct agtgcttcgc caaataaaga ctcatttgga acgcctagca actggctctc
    29761 tccctcatat ccgcccggct ctattactcg taaaagctgt tcctttccgc tagcagataa
    29821 ttgatacact ttcatactgc cggaggccac gatcactaat tgaggttctc cattcggcgc
    29881 aagaatctgt tctccttttt ccacatagtg atgtttgaca agtgcgtgta ctttttcttg
    29941 atcttgctgt actaagtgat tgaacaaagg aacaaatgaa acacataaat gatcatgact
    30001 cataatctct cctccttcta aacagctatt tctaataaag aaacataact tcccttattc
    30061 caactgataa ttttctttta tcatagcata aacgtcaagc gaaccagata ccagcaaata
    30121 gagtggattt ctttttgtcc cttgctttat tttccttctt ttgagacctt aagtaattga
    30181 ttcctatcaa ttttttaaat atttactttt tgaataatca aaaacaaacc ttcactcgca
    30241 agccgagagt aaggtttgtt tttcgcattg cttatttcgt gttgtctatt gaaatttttc
    30301 cgcacctagc ttttgatata gtggtgctag ttcttgtaag gtttcagcgg ttatttgctc
    30361 aacgtgttcg atttcttgcg gatagaagaa tagtttaccc atttgaaagg ttcggtctct
    30421 catcgctttt ttcaactctt cttgtttctt tgtatcggac aaatagtcag acaaagctaa
    30481 gtggtcgtca agttcatgtt ctggttgcgg ccatatgtaa taatccttga tatcgactgt
    30541 tttgctccac tctttcagta tcgcaagcaa ctggttatac gctgcaatgc taccatatcg
    30601 ttcttcgctt ttataatgtt ggtacatcaa atagatttgg tattgttttt gattcaacaa
    30661 aactgctaga caggcattct cgtcttgacg agattcgctt cggtacgcgc tccaaaaatg
    30721 actgcgtaaa ttccagccat tcgtccagct ttcgatttta ggtttgctca tgccgtacat
    30781 atcaggcaaa agtgcagctg tctggagctg aatctctttc catttttgcc agtgttcttt
    30841 atattctttc ttgatttgtt caatcttatc tgttggatgg ctctctttga gctgtttgaa
    30901 tgcgtagatt ggtcgatcaa acaactgata atctgatttt ccaagttcca ttgtttcgcc
    30961 tctttccgtt atcttttgta ttctacctga attttgcgat atggacaaat agaggttgtg
    31021 aaaaagctgt tctgcatcaa gtcataagaa cagccaaaca aaaaactgcg atgagcatca
    31081 ccgcagtttt attgaactta aggttgattg atcacatcta aaagcgttga aaataaaatg
    31141 gcactagaag cagctcctgg gtcgatatgt ccgattgcac gctctcctag ataggaagct
    31201 cgtccttttt tagctaccaa gactttggtg tgttcttttg cctggtcaat gatttctttt
    31261 gttaaattcc ctgccttcaa tgcctctgtg actggcagcc aaacatctaa catcgtttta
    31321 tcgtcagctt ctgcctttcc tcggctttga atacccgctg ttccctgctc gatcacttcg
    31381 cctaattttt cttgagaatc aatagaatcg acctcttttg tcgctttcat catgttcatg
    31441 aatgcagatc cgtataacgg accagaagcc ccaccgactt tcgatatcat cgccatagag
    31501 aatactttga atgtatcgct gattgtttca ggatgatcgg cttgaaaagc ttcttcataa
    31561 gctgaaaccc ctcttcccat gttatttcca tggtctccat cgccaatcgg ggtatctaga
    31621 tcactgagat attgtttgtt ctgattgatt gcctctgcaa attgcgtgag ccattcttgg
    31681 atttcttgaa cggttagttt catattatca cccttttatc ttgagatttt ttaccatgaa
    31741 atcgtctgta cattgctttc taaagaatct ttccattgtg tctccgtcaa gtcgatcagt
    31801 gttagagaaa gaccttgcat atctagtgag gtcataaaat taccgacttt tcggaaaact
    31861 acattgacgc ctctttcttc caacagagct aaaacatcat tcatgaacac gaactgttcc
    31921 atcaaaggcg tcccgcccat tccgttgact agtacacctg cttcttttgg taaattcgag
    31981 taatcatcta aaatcttagt aacaagttcc tttgccagta ctttagaagg ctgcatcttt
    32041 tctcgacgat agcctggctc accgtggata ccaacaccat actcgatctc atcttctcct
    32101 agttcgaatc ctggtttccc tacttctggt acagttgctg ctcgaagcgc tagaccgatc
    32161 gtcttagttg cctgtacgac cttttctccc agttctttca actcagctag ggaagcaccg
    32221 tttcttgctg catcgcccac gattttgtgt acaaggacag tacctgcaac tccgcgtttt
    32281 ccagcagtat acgtactatt ttcaacggcg atatcatcat ctacgatcac actttctact
    32341 tcaatatcgt ccatagcagc taattccttt gccatatcga agttcaatgc atcccctgta
    32401 taattcttaa cgatcagcaa aactcctttt ccactatccg cttccttaat agccgtctga
    32461 atctgatctg gagttggaga agtaaacaca tcaccaagca ccgctgcact taacatccct
    32521 tcccctacaa acccggcatg ggatggttca tgtccgcttc ctccaccaga aaccaaacca
    32581 acttgttccg ctttctgatt cttggctaca acacgggaag attctacacg atgaacaagt
    32641 tctggatagc tttttactaa tccttccagc atttcttcga caatattgct tggatcattc
    32701 atgatttttt tcaatttatt cccatccttt ctttccggca ttttgtgctt ctgttaagat
    32761 ttgctgagta tcatctgtta taccggcagt gatggctgct gcaaaagctc cttccactaa
    32821 aggcgcatcc accagatgat aacgttgctg ctgctcttct tccaacatgt cttttgccag
    32881 ttccgagttc aataccgcgc taccgatatc cgcaaaaaca aaataactag ctgcatcgct
    32941 tgattcattg actgcttcta cgattttcat cgggtctgat ccgatctcgc catttattcc
    33001 gccgagagaa aaaatttgca cctcttcgga tttttgcatc tgctcaatca tttctttaat
    33061 accatctgtt actttttgac tatgagaaat aagcaaaata ctttttttca caagaatctt
    33121 cctttctttt acatagttgc taacgattgg tttcgctttc attaatgaac aattcaagta
    33181 tataataaat ttctaatttt gggaagatat gtgtagtgct tcttgaagtt tgttatactt
    33241 taattaaaag agaggagtga tcgaatgatt acaacaacaa cgaacagtgt agagggaaaa
    33301 gccgtagtag cttataaagg gatcgttttt ggtgaggtca ttactgggat ccatgcattt
    33361 aaagacttgg aggcggggtt gcgaaatatc tttggcggcc gctcaaaaag ctatgaaaac
    33421 gaactaatgg gtgcaagaga agaagcactt gccgaaatgg ccgaacgtgc aaaagcatta
    33481 ggagctgatg cgatcattgg cgtaaaaatg gactatgaag tattaggcag cgacaatggc
    33541 atgctgatgg tcacatgtag tggaacagct gtctccacga gataagtagg ggacatctgt
    33601 tttaataact aaagagagat gggacatgta gtggcactca agaattagat ttttcagtct
    33661 aacttcgagg tactctacct tcccatcctc tcttttttat ttctttttgc ggatgattcc
    33721 gtaagctgct gcgactaaca cacccgcccc tgctaaggaa taactgacag aagctgtttt
    33781 ttcatttgtt tttggcaaag tctgacttgt tgatttggat ggctctgtcg tttgctgaac
    33841 tgtttctgtc gtgcttccag aggtcactga tttttctcct gccgaatgca aaatatcttt
    33901 tattcgttgg aaactagctg ctaagtcagc tttttttgct tcaaaatcat tagcaactgt
    33961 ttgtgcttcg ctgatctttc cgtggattgc ttgactggcc gctgtgatat ccgcttgagt
    34021 ttgacttagc tttccagaag ctgtttgagc aacctgattt aattgctgat tgaattcttt
    34081 tgcttgttgg atcttcccgt caagatctgt ttggacagct tccgcttttg ctttcgcttc
    34141 ggcaagcttt ttctcaagtt gtgcacggtc atttcccact ttatttttaa gatcagctaa
    34201 atcttgatcg attgctgctt ggtcttcttt caatttgttt tttgcagctt ctaatgcttc
    34261 ttcgagttct tttcttttat tttccaagtc ttgcaaatga tcgatttggt cagaagaggt
    34321 agaactattt attgatgtat cttctgaaga tgaaatcgtt tctcctgaat tatctgtcgg
    34381 tacattggaa gtcatttctg tttccgaggc accgttttca gcaccagaag agcttatagt
    34441 tgttgaaggt tctgtttgag caggttcaga agtttccgca aaagctgtct gtcccaataa
    34501 gaaaatactg gaaacagcaa ttggccagat taacggcttc attctttttt tcatcacttt
    34561 tcccatcctt actacttttt aactaatttc ttattataag caaaaatgtt tggaagaacc
    34621 ataaacacaa caggctgttt gtctaaaaaa cggtgcatct tttatttttt tgtacgttcc
    34681 gggcttttcc atgtcccgct tccataacct aaaatcgttt tgactgctgg aatgaaagtc
    34741 gtcacgatcg ttatcgcgtt cactatccag aacaaaagca tatatagtgg tgcgaaaaga
    34801 aggtatttca attttcttcg tcgatcatct acaatcagcg aagctagcaa ctggaaaaat
    34861 cctgcgacca tttcaaagca gatgaacaca aaactgaatg taaaagtaat ataaatctgt
    34921 tcgtagtttc cttgatatac ataatgcccg attaatccag caaaaagaac tacggaaata
    34981 caaaagaata ttgaccagac aatactcaat gtttgatcaa tgaagatgat cgttcttcca
    35041 atatgtttaa aaggatggag catcactttt ttgaaattcg tcagccagac ttctgttcca
    35101 ccttttgccc aacgttttct ttgtcgatat agcatcttca acgtcacagg tacttccatg
    35161 aaaaagataa tctcaggagc aaaaacagat aaccagtcat ttaactgatg atcccaagca
    35221 atactgatgt cttctgtcgc tcgatcttgt ctgaatccgc ccacatcgat caaggcttct
    35281 tttcgataca tcgtatttgc tccactgtac gcataaagac caccgaaaac agctgactgt
    35341 gttcgtttaa tgatgcccac gatacttgag aattcaaccg tttgagattt tgcaatcaat
    35401 ttggtacgat tttggacatc catatttgca gtgaccgcag caatatgtct tgcaccaggg
    35461 cgaatgaaat aattcacata cctaatcagt gcatctggct ccggtaccgt gtctgcatca
    35521 ttgcttaaaa tcaatttccc ttttgcaaag gcaagtccga tattaaacgc gtgggctttt
    35581 cctttatttt tttcgatccg aaccacacga agattagcgt attttttcat cagtctagct
    35641 aatatttctg gtgtttggtc cgttgaacca tcgtctgtca ctaatacctc atagttgtga
    35701 taattcaatt tcgtcatcaa atactcgatt gtatcttcga tcacaatttc ttcattatgg
    35761 gctggcacca tgatcgtgat gaacggttcg acactcaagg gaacatctac ccattctttt
    35821 tgcttatgtt tgtataaaaa cacataacac cagacaccaa taaaccatac aaatccgcct
    35881 aaaatcggat aagaaacgag aaaaaataca taatagacaa acaagtatct aagaataaat
    35941 tacctgtatg ggtgattgag atcatttcag ttccacccca ttttctttat acaaatctgc
    36001 aatgaatgtc gtatctagat ttttctcttc ggagacagaa taaaaacgga ccgtttctct
    36061 ctcttctttt gttccaaaac gttccgtata cacttcttca agtaattctt gtcttttttt
    36121 caatttttca tcttcatatt caaaagattt atttactttt ttgtatagat ttcgattgtt
    36181 ccacctagtc aatacaaatg aggtaatcag gaaactgaaa aaaatcaata caaaaaagcc
    36241 gattaagaaa tacagcattt tcttttctga taaaaaagcg tacaaggaaa aatccttggc
    36301 tctttctggt ctcgttaatg ggaaaaagac ccatataaaa ggaagaacaa cgatcatcca
    36361 tcctaaaaga gccactaacg tttgccaaat ctttaaccag taattacctt ttgaaaaata
    36421 cttatctttc accaaaattg atgcaacttc ttctcttgtt acttttttca tatcctcacc
    36481 tcacgttttt ttgttacgac gtaggagttt accatacatt tcatttcttt tcacataaag
    36541 tgttcctaaa tttttcctaa gaaaaagaac aaaacatttc tcatcagcaa atgaaaaata
    36601 ttttgttctt tttatcagtt gttcttttta ttttttatct cttaattatg ttgctctttc
    36661 caatacgaca atgcttttca ttcctctaga tatcgccgtg tacatttgtc tgcgtcttct
    36721 ttgctcatct ccatcgtttt caaatggata taagattaca tgatcaaatt ccagtccttt
    36781 tgctagactc aaaggaatga tttggcagtt ttctaatcca tatgaatttt ttttaatcga
    36841 ttcttgtagc gtttgggttt cctcttctgt ttgggtgata actgccgctt tttctccttt
    36901 tagagaaacc aaaatctcag ctagtatttt caacaattct tcagaggatc gaaaagagcg
    36961 gtattcgggt tcttttcctt gcggtcggat cgatacgatg tcgatcttct cttgatccac
    37021 agcataggtt ttgaaaagct ctgtgattgc tccgctagaa cggtaactat ttctaagatc
    37081 ataccgatgg atcggcagat ggtagtcgtc gaaacaacgc ataatatcag caaaagtagt
    37141 ggaagaatta aagattgcct gattctcgtc acctactaaa gtaaagtgag tttttggaaa
    37201 taattcgatc aatagaccta attgtgccag ggtataatct tgtacttcat caatcaatag
    37261 atagcgaaat gctggtacat ttaccttttc aacaagtaaa tgacgaatag ctaatataat
    37321 gactgcttca tcaacagtac gtgtgggttg ccaagcataa gctgagccat atcgtttttc
    37381 ataaattctt tcaaacaact gatgctcatc cacccatgcc atttcttcga tttgtctggt
    37441 tatttttcga tatttttttt gtaaaagttt ccttgcataa gctacaatac tttgttccga
    37501 atcatcagaa atcaatttac cgaataattc caactgccta tcttctgtca acgagagtac
    37561 ctgattctgc acagcaggac tttttgcttg tttcaaaaga tgattcttcc attcgctcat
    37621 cagtgccttt ttcgttgctt gaagcctatc gatcaattta ggatgagcag gaactttctc
    37681 atatattttt tcgatcgttt ttttagaaag gactgttttt ccatttttag taagtccacg
    37741 gataaaattc ggatgatcta aaaacaaagg atctgattgt tttagattat cgataaactt
    37801 tttgctacgc aaacgttctg tctgctccga tacattttct cccgtgatac gtttgaaata
    37861 agcctcttca ccttctattt cctctgctga aagctgtgaa accagctgta tgatggttag
    37921 attcagtgga tttctttcac ctaatgatgg taggacattc gaaatatagt cgataaaccg
    37981 atgattaggt gatagaatca gcaagtcatc ggatgtcatc gtatctcgat ataaatagag
    38041 cagatacgca atacgctgca tgatagcaga ggttttacca cttcctgcta cgccatttac
    38101 caagatattt tgacttttca catcccgaat gattgtgttt tgttccgatt ggatcgtagc
    38161 agtgatagcc ttcatttcat tggtctcatc ttggcctaag acttctaata atacgtcatc
    38221 ttgaatagct acagacgtat caaaaaactt gaccaaacga tctctttcca aaagaaattg
    38281 tcgtctttct ttaataaccg tatcgatttt attttgacga accgtataag aagatggacc
    38341 aatcatgtta ttgtaaaaaa gttcggcaat tggtgaacgc caatcataaa caagattttc
    38401 atctgcatca tctgtgaaat ttgctgtacc gatataaaag gattcttttg gctcattatc
    38461 cagaaagtct acaacgactt ttccaaagta gggacttttc aataaacgtt cgatctttgc
    38521 tagctgattt tctgcgtttt tctgtttgat attcatctga tcgatttctc tatttttcat
    38581 ttcaatcatc gaaaaagtat ccaggttatc taagtaactg tcaaaattca atcgaagctc
    38641 gccagccatt tctttcaacg actggtttcc ttctattttt gccgtatcca tcgattcccg
    38701 cagttcgttt tttttatcaa ttagcttttg atacacgttc ttcagatatg cttcttctaa
    38761 ctgtttttgt gtgttgacca tacacattct cctctttttt taaagtggtg gaaccgatta
    38821 gtttacctct tttaagagtc gaatgcaagc tttttaacga gtatcatttg ttaggatata
    38881 tcagttcttc aggtcggaca gtttgatata aagaaaatgc accatcaagg ttttttacgt
    38941 ggtagccaga ctgcttcaag attctttccg ctagatagct acgtaatccg ctatgacagc
    39001 tgacgatata ttcttgttgg ctgtctaatt catttaatcg gtcccgaagt tcattcaaag
    39061 ggatatgttt ggcttccttg aatttgccct tttccaattc ttctgctgtt cgcacatcta
    39121 ataaaatttt tccttttgct agttctgtcg gcaattcatg ccattgtacg gtttcactaa
    39181 gcccttctgc aatgttcatt gctgcatatc ctagcatgtt tactggatct ttggctgagc
    39241 caaatggcgg tgcatacgtc aattctaatt ccggtaaatc gaagatcgtc aaatgccctt
    39301 tgattgctgt tgctaaaata tcgatccgtt tatccacacc ttttgcccca acaccttgtg
    39361 caccataaat ctcgccagtc tctggatgga agatcaactt cagtaaaata tctgttgctt
    39421 ctggataata gcttgcatga tcttttcctg atacatgaat gacttcaaaa gacagttcgt
    39481 tttctttggc tgttctttca cttaaaccgg tggatgcggc agtcattcca aacgttcgta
    39541 taattgctgt tccaataccg ccttgatttc ttcgagaaat tccggaaatc gtgtccgcaa
    39601 cttgtctacc ttgacgattg gctggtgaag caagagaaat aagtgcatct tttccagtga
    39661 tttgctgttt tacaacgata gcatccccca ccgcataaat atcaggaata ttcgtttgat
    39721 aacgttcgtc tactaggata cccccgcgta tgcctaattc gattccagcg gtcactgcta
    39781 gctggttatc aggacgaaca ccaatggata aaataactaa atcagcaggg atggtacgcc
    39841 catcatccag tcgcaatcgt ttcccgtggt cttcaaatcc agcgacagca tgagaagtaa
    39901 tcacttctac tccttgatga gacaattctt tttctataaa agctgccatt tcttcatcca
    39961 gtgttggaag aatatgtggt gcttgttcga tcaccatgac ttctaaacct cttcttttta
    40021 ggttttctgc catttccaat ccgatgaatc ctgcaccaac gatcacggca cgctttggct
    40081 gtttttctaa tgcatgcatc actttatcga tatctggtac atttctaata gaaaaaacat
    40141 tgtcggcttc tgttatccct ggaatcgatg gaacaactgg ttttgcacca ggagataaaa
    40201 ccaatgcatc gtactgttca atgtgttttt gaccctcatg tacgacttct accgttttgt
    40261 tttcgggaaa gatggctgtc acttcatgat ttggacgcac atctaaacaa aaacgagcgg
    40321 ataatgattc tggcgtttgg acaagaagat tttcccgttc actgatttct cccgaaagat
    40381 aataaggcaa gccgcaattt gcaaaagaaa catacggtcc tttttcaaaa acaacgattt
    40441 ctgcatcttc cattaatcgg cgaagccgtg tagcagcaga cattccacct gctacacctc
    40501 cgacaataac gattttcatc ttatttccct cctactgttc gatcaaacca ttgattcatg
    40561 ccaccacgta cattcactac atcataacca gatttcttta attctttagc tgcttgttta
    40621 ctcctcatcc ctgattgaca aatcacatag acggtctttt ctttgtctcc atcatatctg
    40681 tttatacttt gtaatggaac atttttagct ccttttatat gcccgccccg gtattctgca
    40741 ggtgttctaa catcaagtaa cgctggattt tccaaaagct tttcctttaa ttctgggact
    40801 gttatcgatt ttattgattg aaatagcaaa ttcattttct gcacctcatt tacattttta
    40861 taaatacctc atggggtata ttaaacagga gctttattaa tatgtcaacc agaatgactc
    40921 ttcatttttt tcactacttt ttatatttgc ataaaatacc gataaggggt attatctaat
    40981 tataaaaata gaggagaatg tgttatggag attgataata aaaatatcct gaaccgtttg
    41041 aaacgtacag agggacaaat tcgtggtatc caaaaaatga tcgaagacga aaaagaatgt
    41101 atggatgtca tcactcaatt aagcgctgtt cgttctagta ttgatcgcgt aatgggcatg
    41161 atcgttgcag ataatctgaa aaattgcttt gagaaacctg atagtaatcc agaagaacaa
    41221 gcggctaaac tagaacaagc gatcaagatg attattaaaa agtaaaggaa tgatttcaat
    41281 gaaagtcgtt ttccatattg atgaattaga aaaatggtca gaaacaggga aaaacgtaaa
    41341 aaacctgata aaagcttctc cagaaacgac gattgtcgta tctgtaaatg gcatcgcgat
    41401 taccggttat ctagactctg ccaatgcaga attcttagat ttgcaaggag tcgtttttca
    41461 cgcttgcgcc aatgctatgc gtgctaatca tatttcagaa agttctttac ctgaacaagt
    41521 aatcgttgta ccagcaggtg tccttgattt agttgaactg caatcccaag gttatgctta
    41581 tatcaagcct tagtcagtag aggttgcgac agaagtgatc agcttcaaga aatttcggct
    41641 tatttttcga agaagctact tctggaaaac cgtgaatcaa aggttgtgaa aacgctgtcc
    41701 tgcatcaagt aataagaaca gccaaacaaa aatagcttct catatttttc gtttggttgg
    41761 gcttattacc gcagatgcaa gcttttgaac accgtttaag gaagaggttg tgaaatcagc
    41821 ggtttgacct aagtattaga gaaaaaaacg ggaaaatagc tcctcatatt ttgccgtttt
    41881 ttgagctaat accgaaggtc agctgattga acaccgttta aggaagagat tgtgaaaaag
    41941 tcgcttagtt tcgagtatta agaaaaattt tgtgaaaatg gatttctaca tttttgcata
    42001 aatttggctt aataccgaga aactggcttt tgaataccgt ttaaggaaga ggttgtaaaa
    42061 tcagcggttt gacctgagta ttagagaaac aaacggaaaa atagctgctc atattttgcc
    42121 gttttttgag ctaataccga aggtcagctg attgaacacc gtttaccaag aggttgtgag
    42181 cctaccattt aactccgaat cacaagaaat aatttcaaaa acaacttctc ctgtttttgg
    42241 tttggttggc ttattatcga gaagtagcta ctgggagtct atttatcaaa aaaaggaatg
    42301 cgacataggt cacacccctt ttttattttc tttgaaacat ctttcggtac tcgacagcat
    42361 acaagacaaa agattgtaaa agaccgtaag tgatgacggc aaaaatgact ctcacccaca
    42421 gcgttggtaa ttctgggaaa agcagcaaaa ggatcatgcc aacatagatg acagcagtcg
    42481 ctactttgcc ataccacttc gctccactta atttgatatt tttagatagc ccgattaatc
    42541 cagctacaaa cataaagcct tcttttatca agaatagacc aaacaacagc caaagccacg
    42601 gtcgatacca tgccatagac aaaatcagaa aaagttgtgt caatttatct gcaaacggat
    42661 caagcacctt ccctaagtct gttacctgat tatatctccg agcaatgacc ccgtctagat
    42721 aatcagacaa acctgagaca acaatgaccg ctcctgctat ataaaatgac tgtctcagca
    42781 caaaataaag ataaatcggc agcaaaaaaa tacggaatat cgataaaaga ttcggaatcg
    42841 tttgtatttc tttacgccag tctgtgctta atttcatttt atcgccctac ctcctcagca
    42901 ctcttttctt tttattttag cttacacggg gaataaaacc aatgatttca ctgtttttat
    42961 ttttctttac agccactgat attatgagct aatttgagat aatcacttgc tgttttcatc
    43021 gtttactctt ctttcttcct gtcgtttgcg catttctttt actagtactt tatgagcttt
    43081 ttttgatcct cgctctaggt cacgctgccg tttacgtgtt ttataatcga taaacagact
    43141 gtctaaatcg taacctgtcg ggtacagatc ttctgctgaa cgcaccagac gaacacgctg
    43201 acgaggcacg tggattattt ttttgtctaa atatacatca atcgtatctt cacctatatc
    43261 ttcataaact aacgcttctt tttggtattc attgacaaat atccgatcac cttttgcaaa
    43321 gagctggcgt tcttttttat tctccttcct atttgtaact gctgtcttag gaagaaattc
    43381 ttttttctgt gtccgatagg ctttttccgc caagtaacgc tctgcttgat ggatcaattt
    43441 catcgacata tccattttat gagcaatcca caaagcttgg ctttctcctg tttctccaac
    43501 ccgcaactga tatttaggac gaagtgcttc ccgatcgaaa gccatagcag ccggcacaaa
    43561 atcttcatga tctccggcga atttcttgat ttctccataa tgggtcgtgg caatgatcaa
    43621 tgccccttgc tcatacatag attccatgat tgcgatcgct agtgcagccc cttcatttgg
    43681 ctctgttcca ctgccaatct catctaaaag taccaacgta ttccttttta tctttttcaa
    43741 aatagcagcg atgttttgca tatgaccaga aaatgtactc aacgcatttt ctaaattttg
    43801 gtggtcaccg acatcgacga aaatctcatc aaagacagca agctctgtcc cttcttttgc
    43861 cggaacttgc ataccgaaca tcgccatcaa agtcaataaa ccgactgtct tcaacacgac
    43921 tgttttgcct ccggcatttg ctccggtgat aatcaagcct cgatagtctt taccgatttc
    43981 aacatccaat ggtaccgcat gctcaggtaa aaatggatgt ctgccttgct tgataatgat
    44041 atgttccgat ttattgattc taggtgtgct tccattgatt tctcggctaa attttgcccg
    44101 agcaaagatg atatctaaca ccgtgatcgt ttcgatcaat aaatcgatca cttcttcgtt
    44161 ttctgcgata gcaccagtca atgccgccag gacttgatat tcttcactga tttcttcagc
    44221 ttttaacaac tgatagtgtt cattcaattt agaaatcaca gttggttcaa taaaaaccgt
    44281 cgttctttta tttgattcat cgataatcgt tccatctatt ttatttttat agctggcttt
    44341 gataggaatc gtatagtatt cgcctttttg aacaatcatt gcttcttgga tcatttcttt
    44401 gtttttcgga tgacgcaaga atttcagtaa ttttgactgg atttcttttt cgattagttg
    44461 taattgtttg cgtactttac gcaaattacg tgacgcgtcg tcactgactt tattattttt
    44521 tattttctgg tcaatcaact cttctacatt tatcaaatca ggaagatgtt tgctataagc
    44581 aaacaacaag ggtgtctggt attgattctt atcaaaaaac tttgtgatca tccgactgct
    44641 tctaagaaaa tccgcatatt cgatcaaatc ggttggctgc agaatcaacc cttttttgac
    44701 ttgttctgtc agtgcatcga tcctcggcaa ccccataaat ggaacatgct gactgctttc
    44761 taagatcaag cgtgcttctc ttgtttcttc ttgacgatcc aagacagttt gtaggtttgt
    44821 cgagaccgtc atatcgctga ttcgtttttt gctatagttt ccaattgctc ttgcttggat
    44881 ttcctttttg atccgatcaa attgtaattg actaatcatt tcgttgtcca tattctctcc
    44941 ttttcttgaa gagatacctg tcccaaaata aataagggcc tagagataaa taaaaggata
    45001 gaacacagct taaaaaagtc atgtcctatc cttattagaa tacaaagata ttgttttcct
    45061 gacaggtacc gtttcataag cacaccaaat aaactttgtt tattggctat tcgacttact
    45121 gattagatac cagctacact cacaaaacat accccattct tctaggtctt atttattttc
    45181 cacttaacta tagcaagaca cattttggtt gtcaaacttt agtatgtttt atagatttac
    45241 accattaatg aatagacacc taccgcgata attgctccga tcattggacc tgcgattggt
    45301 acccatgaat atgcccaatc tgaagttcct ttattagcta ttggcaatac cgcatgtgct
    45361 aatcgaggac caaagtcccg agcagggttg attgcatatc ctgttggccc tcctaatgaa
    45421 agaccgatgg ctaagatcaa tccacctaca gcgaatacgt tcgttccagg tgcaaattcg
    45481 ttttgtccaa aagctagcaa cccaagaact aatacaaatg taccaattaa ttctgtgatc
    45541 acatttgctg gataattccg tatagcagga cctgttgcaa acgttcctaa aatcgcgctt
    45601 tcatctttcg ttgcattcca atgtggcaaa taagaaagcc aaacaagaat ggcccctgca
    45661 aaagctccta aaacttgcgc aatgatgaaa ggaacgacaa gattccaact aattgcccct
    45721 gtcatcgcca tagctagaga gactgcagga ttcaaatgag ctgggcccat gaatccagaa
    45781 atatagacag ccattgttac agcgagtccc caaccaaatg cgatgacaat ccagccagaa
    45841 gcctgtgctt tacttttgtt taaattcaca gccgcacaca caccatctcc aagcaaaact
    45901 aggatcagtg tcccaataaa ctctcccatg atttgagtca tatcactgtt catttatttc
    45961 tcccccttta gttcccgcag atctgattct gcaatcaatg cttctaaacg tttagtctgt
    46021 tgcgccttct cctcttctgt ccattcaaaa tatccagcaa ttgcatcgat cacaggttgc
    46081 ttgatctcat caagctgatc tcgttcaaac aacagatgat ttgtacgacg aatgagataa
    46141 tcgcctgggg ccaaaaccat ttcttcttct aaaccataac gtaaccgagc tgactcagcg
    46201 agactcaaac caggataagg tgccatttct tttgctaatt caaagatacg tcgggcatta
    46261 gtcccgtaaa aatcagcgat ataagtagca tcttcttcct ctaaaccggc tgctactcct
    46321 tcttttgcta attctgtcac tgtttcttct aatttagttg gatcgaagtt tccacctgaa
    46381 atctgatatt ttttagagtc gatttctttc gtctctattc cgtattcttc ttttaataat
    46441 tggcgtatca atcgcaaagc tccttctgcc atcttacggt aatcggtgat tttcccacca
    46501 gataaagtca gcaaaccatc tggttctctt tctaaagaac tacctctgga aatcgtagaa
    46561 ggtgcttttt catcacggct gttttccaaa tggttcaaca cgtcttctac ttcagcacga
    46621 gaaactttgt tttccttata ttcacttact gtatcgacaa ctttattgaa gctcttgtct
    46681 gaaatcgatc cattatctcc accattgtaa tcagaaccag aattaccaat caacagtgga
    46741 cgaagtcctg cccagcttgc ttcgatatca ttcaatgtga tatttgcttc tggatagcga
    46801 tgattgatca catccaatag ataatctaca tcttcttgtg tgactttcgg atccgtaaaa
    46861 tctccttggt aatccgtatc tgtcgtacca aagtaagtct tgttttctct tggaatagca
    46921 aaaaccatcc gcccatcttg ctttcctgta tcgaagtatg tgggttgcgg tacaggcagt
    46981 tttttcgcat ctacaactaa atggatccct ttagttggac gcattttagg agagactgga
    47041 cgcgtaaaat tcaagtttcg tactttatct acccaaggac cactcgtatt gatcactaat
    47101 tttgctttga tttcgatcac ttcatctgtc agcaggtcac gagctttgac gccaacgatt
    47161 tgatcgccat catataaaaa gccaaccgct ttcattttac ttactagata agccccatct
    47221 tcggcagcct ttttgatatt atcaatcact aaacgggcat cattgttgcg gaaatctaga
    47281 tagacacccg cgccttttag cccttctttt ttcaaaaacg gttctctttc caatacttct
    47341 tctggtgtca gcgtataatt ctcatatttg gttcctgtca catttgccag tttgtcataa
    47401 aggtccattg ctactttgac cgagaacata ttgaaggttg ttgctccttc gccctcatag
    47461 attggcaaaa gcattggatc tggttttggg atgtgtgggg caatactttg tacgactgca
    47521 cgttcaccaa ctgtgtcagc tactacttcc acatcaaatg tcttcagata acgaataccg
    47581 ccatgaacaa gtttggtcga gcgagaggaa gtcccttctg caaaatcctg catttcgatc
    47641 aatcctgttt tgattcctga tgctgctgac tggactgcca caccggcacc agtgattccg
    47701 ccaccgatga tcaacagatc caattcttct tgctgcattt tttgaatgct atcttgtctt
    47761 gtcttgtttg aaaacatatg gatttcctcc ttattcaaat gcttgggtag cttttactgc
    47821 ttttttccaa ccgttgtaaa gtttttctcg gcgctcttcc tccatttgaa cttcaaatag
    47881 cttgccagct tcataaaatt cgcggatctc gtctgtatct ttccaataac cgacagctaa
    47941 tcctgctaaa aatgctgccc ctagcgccgt tgtttccaag ttttctgctc gttggatcgg
    48001 tacattcaag atatctgttt ggaattgcat cagatattca ttgtttgctg caccgccatc
    48061 tactttcaat acaggaatct tgattccagt gtcatcttgc atcgtatcga tgatatctct
    48121 cacttgataa gcaatcgact gtaaagtagc tttaatgata tcttcttttg tcgtaccacg
    48181 tgtcaagcca aacatagccc ctcgtgctgc ctgatcccaa taaggagcac ctagcccaac
    48241 gaatgcagga acaacataaa cctcatcttc gcttgtagag cgtttcgctg cgtcttctga
    48301 atcgctagct ttttgcaaca tttgaagtcc atctctcagc cattgaatcg aagaaccagc
    48361 cacaaaaata cttccttcta aagcatagta aacctttcca tttatgccgt aaccaattgt
    48421 cgtcaataaa ttatttttgg ataattgcgg tttttcaccg gtattcatga caataaatga
    48481 acctgtacca taagtattct tgaccatgcc tggttcgaat gccatttgtc cgaataatgc
    48541 ggcttgctgg tctcctgcca tccctgaaat tggtgcttcg cttccataaa aatgataacc
    48601 ttgcgtataa ccataaactt ctgagttaga agtgactttt ggtaacatca ctcgaggaat
    48661 atttagtaaa tctaaaatct cctgatccca atctagctga tggatattga acaacatcgt
    48721 ccgactggca ttcgagtaat cagtaacatg tgcttgtcca tcggtcagtt tccatactaa
    48781 ccaagaatcg atcgttccaa acatcaattc gcctttttca gctcgttctt gcgcgccttc
    48841 tacatgatca agaatccagc gtatcttcgt tgctgagaaa taggcatcga tcactaaccc
    48901 tgttttttcg tggaaaaatt cttgatagcc ttcatttttt aagttatctg caattccagc
    48961 tgactgtctg gattgccaaa caactgcatg atagattgga cgtcctgtat ctttttccca
    49021 tattactgtt gtttcacgct ggttcgttat gccaatccca gcaatctgat ttggtttgat
    49081 ccctgattcg atgaacgctc cggcaataac tgactgtaca gaattccaga tttcatttgc
    49141 atcatgttcc acccaacttt cttgcgggaa atattgcgtg aactcttttt gagaactgcc
    49201 gatgtgtctt cctttcttgt caaagataat tgctcgtgag ctagttgtac cttggtcaat
    49261 cgccatgata tacgttgatt ctgtcattgt gattccctcc attatatgca aacgctttct
    49321 ttaactactc ttatcatact actgttttaa aaaaagaaac tgtcaaaatt ttattctttt
    49381 tttctttttt tgacagtttt ttattatgaa gctatttttt aatcaattct tttctttttt
    49441 cagcgtctct attcttcttt tcaatcgaat aatgtcgtaa ggtgacgcaa attctgacag
    49501 ataatattca cactgttttt tgtttctctg gcaaaatgtg atcaccaaat catattcttt
    49561 accttcttca taacttccaa tctcaatacc gccgattccc ctcagttctc tgatcagata
    49621 ttgttgaaaa gcaatacgat aaatcggcag actttctaaa tcgattccaa tcactactgg
    49681 ttttgcaata taaaaatcga tcatcaaaag aacattggca tactggatca tcaggtaact
    49741 aagctcacta tcttcagctc ttttcctcgg caattgctgc accgctgttt cctgtaattt
    49801 cgccagtaac tgtgttaagg aatgcccgag cattttctgc tgctgttcca aaagccgatc
    49861 acgctcatag acttcgatct ttccaacaaa gaaaaaccat tcattgttga tctgggaaat
    49921 ttggtagcct actgcctttt cttctttgat tttcaaacga cgaggacgat aatgccataa
    49981 catcgtttcc cgaatgtaag tatctaaaac agcggttggt aactttttgc tccgtgtcaa
    50041 atcatactga taaaatattt cttcgtctac tatcgaaaaa gaaacaaaaa agctgtaaaa
    50101 catcattgtt tcgtatacat tcagttctgc tgctgttcga ctaagatgca tcacgatgat
    50161 tggatcgatt gcttgataaa gccggtccga ctgatagagt aatttctttt ctttcaaagt
    50221 tgcgtacgta cttttttcat tcaatagtct ttttttcgta atcccgagcc agcaagcaat
    50281 ctgtgcttct gctgatgccg taaacgttgt ttctaataca cggtccaatc ctagaatgaa
    50341 cggtcttttt tctggtgtat tttgcaaaaa aagcggtcgc tgatcttccg ggatatattg
    50401 gaatagttca taataaaaat atcgtatctg tagctcttct ccgtataatt gaccgttttt
    50461 gatttgtaat ccaaattcct ctaagagctg gttcaattct ttgatctttc gaaaaacaga
    50521 agactcactg atattaaacg ctgtcgctaa tcgaacgatg gatacttgct gatgttctaa
    50581 taaatagacg agcatcttaa acttcaagct ttcctgcaat aaaaacaata gaattttttc
    50641 caaacttaca tcttcgtcca acacaagcag cgcctgatac tcatttcgct gtacttctac
    50701 ttttttttcc atcattcggc caatcaattc aatatcttcc aaatattgat caaacgaggc
    50761 ttttgacagt tggacatgct ctctcaaacc attgatagag gctgttcccc cttgcagaac
    50821 aaggtttcgc aaaatcagga tctgtaactg ctcttttctt tctaataatg cttcaatctt
    50881 cataggcagt attcgccacc tttgaccaca ccatatctgg cacacggtat tctggctgga
    50941 aaaatagctc atgttcttct aactgttcaa acgtccatcc ttcttctact gcaattgtca
    51001 gttggtcaag aaggtggaac atctcttttt tgctttggag ctgtgctcca agaatctttt
    51061 tttcagaatc aaatagaaat ttgagtttaa ctttgctttg atttattgct gtaagcttgt
    51121 agtctacgta tcctgtttca attttttggg gataaaaaat gctctcttct tctgtcaaac
    51181 ctacgcttcc taagaaatag ccaaaccact gattgccgac tggacgatag gttccctgat
    51241 cctttgtctg tgagccacta attgtcttgc tgacatttcg tgctgtacgg acagcgttat
    51301 ttactagaga aacatacatt ttttcatttg ttggacgaaa agttacttgg atcgcatcgc
    51361 caatcgcata aacgtctttt tgagacgttt gcaagtagtc atctacccag atcgttccat
    51421 catcattcaa tgttaattgt tcttcccaca tctgattgtc ggggcgagaa tgattagcca
    51481 gcaaaacaag atcatagttc tcctttcttt tttccgtctc tacaatagcg gtttcatttt
    51541 cggtcaaaga cacaacttgt tcttctaaaa aaagtgaaag cccttgattt ttcatttcct
    51601 ttactaacgg ttctgtcatt tcaggatcta gataacgaaa aagaattgac tttcggcttt
    51661 cgtaaagatg gatttgtttt tttcttgatg ccaacccttc agctatttcg attcctgctt
    51721 gtcctgcacc aataattgca attttctttg cattcatgat cttttgctga agagccgtta
    51781 cgttttgtat ctttttaact gggtgaataa gcgttgattc tgcagaaata tttcgaaact
    51841 tttgatttga accagtcgct agaatcaaac gatcaaaatc caattttttt cctcctgcta
    51901 aaacgacttt tttttctttc aatcctatta ctgtcttttc tgtgtaaaca tggattcgat
    51961 agcggtcagt cagttcttct ttcgtgatcc aagaaagttg tttttcactg gtaacctttc
    52021 cttttaatat caaattgaac ccgctgggaa tccatcctaa cttcgattgc ttttcaatca
    52081 aatatatttc acttgttggc atcaattggc gaagataaat agccgcatga atccctgcaa
    52141 acgacccgcc gacaattaca atgttcatct gcattcctct ttcttataag gtgttctact
    52201 gctaacttct caatcagaaa acacagtatt cataactcgc tttcaacctt atgatacata
    52261 gcgatgcagc agaatcaacc ctctccatca taccgtgttc taggtctgct tctcaataat
    52321 gtactagtgg acctaattga agagtatcat ttctgaagag atgtttcaaa aaatgtactc
    52381 ttataaaaga aaaaaactcg ccttttacgt acctctgtcc aaataggaaa cagaaaagaa
    52441 cgaaaagacg agttttagcg tgtgtaatgt aatagttaga atagacacat catttatttt
    52501 taattcttta atgcttcaac aactgcttcg cctaattgat gagcagattg aggattttgc
    52561 cctgtaagaa gccgcccatc aatttgaacc acttctgaaa aagctgctcc agactgatag
    52621 tttgctcctt tttctttcaa agcatcttct gctaagaatg gtacttcatt cgttgtacca
    52681 tttgcttcct cttcttcatt actgaaccca gtcactgtac gtccagctaa aatagatttt
    52741 ccagactcat ctgtaactgg caacaatcct acaacaccgt gacaaacagc tgagaccagc
    52801 ccttcatttc gatagatttg tgaagcgact ttcccaagat ctattgcttc tggaaaatcc
    52861 cacattgttc cgtggccacc agcataataa accgctgcat aatcatttgc atccacatca
    52921 tcaggacgta aggtatttcc caaggcccga tttctgaaat cttcatcttc ataataggcc
    52981 caatcgattt catccatatt caaaacactt tgcggttcta aaggtacata tccgccttta
    53041 ggactgacaa aatctgcatc gatcccattt tttactaata catcataaaa atgagtcagt
    53101 tcacttaacc acaaacctgt aggacgctcg atcttttcgt acttttctac atttgttaat
    53161 acgatcaata ctttcatttt cccatcctct ttctataaaa attgcgttat tgataacgtg
    53221 ctttcaagaa attatataat gggcttggca aataaagctc tttatatgct tcttcaaaag
    53281 gtttcgttac aaattcatcc tcacggtttt ccaataattt ctctgcccag tcaggatcga
    53341 cgatcatttg ctgtccaatt gctgcaagtt ctgcatattt caatacctgc tctacatctt
    53401 cgcgattgcg gatcccaccg acaccaacta aagggatctt tccttgaatc gtgttatgga
    53461 ccacttcaag aatcgattta tcgttgtatt tatcagaacg agctattcga tcatacatat
    53521 ttaatgaaac atgcagataa tctagttttg actcacgtaa tcgttcaaga agccagatcg
    53581 tgtcttcaaa acggatacct ggtgtttcat attcttctgg tgagaaacga taccctacga
    53641 tgaatggttt tttagcatat tgttcaaccg ctgagaaaac cgttgttaat aattcttcaa
    53701 taaaacgata acgattttcc aagctaccgc cccattgatc ctctcgtcta ttcgaatgcg
    53761 gagagaaaaa ttgttggatc aaatacgtat ttgctccatg caactcgatt ccatcaaatc
    53821 ctgattcaat cgctctacgt gtggctttgc caaatgcttg gattgtttca acgatttctt
    53881 cttcactcat tgctcgtggt gtttccgcat caggacgttt tgctgcaact gcacttgcag
    53941 atacaggctg ttctccagat aaagttgctc gacttgtcat tcgtccaccg tggaaaatct
    54001 gaataatcgc ttttgcgcct tctccttgga ttgctttcgc tagctcactt aatcgaggaa
    54061 tcatttcatc atgagcgatg ctgagttcac ctggccagcc ttttccgata tcttggacat
    54121 ttgcagcacc agtaataaca gcaccaagac ctttagaacg ttgtttataa taagtgatct
    54181 catcttcggt gatcactcca ttgaaaaagc tgagttgtgt cgtcataggc gacagcatca
    54241 atcggttctt caattgaact cctgaaggta aagtaattga ttgatcaaat tgtgacattg
    54301 tcattcctcc cataattcgc tctgattaac ctatcgagaa tcatctatca ttaaaccact
    54361 cgattagttg tacatgcaac taatttgctt aaaaaatttt attttttttg taacttatct
    54421 gttgcatcat tcattgtctg ggctaattct ttacttgctt catttcccaa aactgctttg
    54481 tatgactgat gaagttcttc ccagctacca tcgacttcct tagcaaattg gcgtccttgt
    54541 tctgtaagat aaactaatga caagcgacct tcgctaactg ttttgacgta tcctttatat
    54601 tctaattttt caacaaaacg agcaatcgtt gatggggcgg aatagagcat ttgtgcaata
    54661 tctttttgtg gtattccatc tttttctttt aatatcaata aaataaaacc ataagttggt
    54721 gccagacctg tccttctaaa cgcttcatca gctaatttat tcaacatgcg atctagcttt
    54781 ttcacgctaa agaaaagaca ttcatctaac gctttttcaa ttggcttttc catttcatcc
    54841 cctccattgg cgtacatatc atagtatgat ttttattttt tgtcaaaaaa cacggaataa
    54901 tgtccgcctc ctctgtctag atttgaaaaa aagaagtagc catcttcaaa gaatttcggc
    54961 ttgttttcga caaaactcct tctggaacac cgtgaatcag aggttgtgaa aaagtcgttt
    55021 agttttgagt atgaagaaaa attttgtgaa aatggctttc cacatttttg cataaatttg
    55081 gcttaatacc gaaaaactgg cttttgaaca ccgtttatat cagaggttgt gaaaaagccg
    55141 tcctgcatca agtaataaga acagccaaac aaaaatagtt cctcatattt tgtcgttttt
    55201 tgagctaatg ccgatggtca gctgattgta caccgtttat tgagaggtcg tgagcctgcc
    55261 gttgaactcc gagtcacaag gagcaatttc aaaaaacagc ttctcatgtt ttatgactat
    55321 ggtgacttgt gatcgaggag ttggcaggag aatactgttt attcgtaaag aaggggctgt
    55381 gacaagactt ttgtcacagt cccttatttc cttttattat aggatttcgc catttgattg
    55441 gatgacttgt ctgtaccaat caaatgattt tttctttaac cgtttctttg tgccattccc
    55501 ttcgtcatct tgatcaacat agatatatcc ataacgtttt gacatttgtg aagtagaagc
    55561 gctgacgata tcaatgcagc cccacgacgt ataaccaaat aaatctactc catctttgat
    55621 agcttccttc atctgctcaa tatgctgttt caaatagtca atgcggtaat cgtcttgaac
    55681 ggttccatct tcttctactt tgtctatagc tccgagtccg ttttcagcta tgaacagtgg
    55741 tttttgatac ctatcatata attgattcaa agcaattctc aaacccttag gatcaatctg
    55801 ccagccccat tcacttgatg gaagatacgg atttttaatt cctccagtta ttaagtttcc
    55861 ttggacccct tcccaatttt ctggatgagc actgactgca ctagacatgt agtagctaaa
    55921 agaaacaaaa tcaacagggt aggctgctaa gatttcttca tcacctactt ctatttttaa
    55981 gttgacacct ttttcttcaa acattcgttg cgtatacgct ggataatatc ctcttgcttg
    56041 aacatcacta aagaacaaag aagttctttg ctgctcaatt gttttttgaa tatcatccgg
    56101 atcacaagaa tacggaacaa gaagctgata gctgatcata caaccgactt gtgaaccttt
    56161 gataatttca tgacaagctt ttgtagctaa agcacttgct ataaactgat ggtgtgcacc
    56221 ttggtatttc gcttgttcca aatggggatg gttttcttca attactccta cgctaacaaa
    56281 agggtgatgt tttacacagt tgatctcatt gaaagtcatc caatatttta ctttattttt
    56341 atagcgtttg aatactgttt cagcgaaacg aacgtaaaac tgaataagct ttcgattagg
    56401 ccatccacca tattctgtaa ccaaatataa cggcatttca taatgggaaa gagaaacgac
    56461 tggctcaatc ccatatttta gacattcgtc aaacacttga tcataaaaaa ataatcccgc
    56521 ttcattgggt gtttcttcat caccatgagg aaaaattctt gtccatgcga tagacatccg
    56581 atagcattta aaacccattt cagcaaataa acggatgtct tctttaaagt gatgatagaa
    56641 atcatttccg tgtcttttag gataataata cttgctgttg ggatcctgcg ccgcttttac
    56701 ttgctgatgc gtcatcatat gccattgatc tgtccatttt tctttaggta aatcgctgtc
    56761 gaatgtatac acatcagaca cagataatcc tttttcatct acatcccatg caccttcaca
    56821 ttgattggcc gctgtcgctc cgccccataa aaatccttct ggaaattttt tcatatcgat
    56881 ccccctaaac ttgctagttt tcttgtttga ttatttttct tatgactagt aatgatattt
    56941 ttgaatctac tactatttgt aacaataacc attgtggtcg gatcatatcc ctctttgatt
    57001 aattcttgca aatctacttc catcaataaa tcgcctttac gtactttttt tccatcagat
    57061 actgcacttt gaaagtattt gccatttaaa ttcactgtat caataccgat atggatcagt
    57121 atctctgctc cttcttcact gactacccca attgcatgtt tcgttggaaa aactgttgaa
    57181 acttccccgt ttacaggact gtagattttt ccgtccgttg gaatgatcgc aaacccttct
    57241 cctaaagatc cactagcaaa tgttggatcg tttacttcag ataaatcaat tatttcacct
    57301 gctgcaggag tacttaacac tatatccttt gaggattctt cttcgatttc atccttgtag
    57361 ccgaaaagaa aagttagcat acaggaactg atcatcgcta agaaataact agcaactagc
    57421 caaacaaatc cttcaccaat ataaatcggg aaagacaaaa tacttcttgt agcaactgcc
    57481 aaagcaccgg ctcctgcggc accagctact ccaccaaata tagctccaca tatacaagca
    57541 atgtaaaatg gtcgtttata tttaagagtc acaccataaa cagcaggttc agtgattcca
    57601 aataatgctg tgataaatgc agataaagat aaacttttta ctttcttatt tttcgttttc
    57661 aaaaataccc caagtactgc tcctgcttgc gcagctacgg cagtcataca tgctggacct
    57721 agcgtatcac gaccgtagaa agaaagattg ttggtcatta ctggtacaag tccccaatgt
    57781 aacccaaaaa tcactaacac ttgccaaaat ccgccgataa cagcaccaga taaaacaggg
    57841 ctaaagttat aaacagctaa atatccagct gcaatcagct gacttgcata atctgcaatt
    57901 ggacctacaa ctaataaagt aattgggacc atcacgatga tacaaatgaa tggtgtcaat
    57961 aaatttcgaa tactttcgtg caagcttttg cgaatcttag gctcaatata agaaagaacc
    58021 catacagcta aaattgctgg aataaccgta cttgtatacc ttgctagaat cacgggcatc
    58081 cctagaaatc gcaaggttat gctatcacta tatgcactga caatcgtagg ataaacgaga
    58141 gctgctgcaa taaccatagc gatgaaacga tctgtattga acttttttgc agcagtatat
    58201 gccaaaaata taggcaagaa atagaaaaat gcatcagctg ctgcattcaa aatgatataa
    58261 gttcctgatt cagtagttaa taaattcaaa ctcgtacata aggcgagtac tcctttgatc
    58321 aaaccacttc ctgccaagac gcctaaagtc ggggcaaaaa cagcggcgat catgtctata
    58381 aaagagttta aaatgccttt tttctcagtt cctctctttc cactagatgc atcatcttct
    58441 agtttcattt gtttggataa tacgtcatac acttcattta ctgcatttcc tactacgact
    58501 tgaaacattc ctccagcttc gactacagtg atcacacctt ctagttgctc taccttctcc
    58561 ttatctgcct tcttattatc ttttagggta aagcgtaacc ttgttgcaca atggtaaaca
    58621 ttgctgacat tttcttcgcc tccgacaaat cggacaatat ctttcgctaa ttgtgaatag
    58681 tccattttta cttccccctt gttaattaat gccgtagcaa tccttcttac aagataagta
    58741 tagttcttag aaaacggttt ctccattcaa ttagtacccc ttataatagc gaacgaaact
    58801 gtacaatagt gtgtatggtt tacttagccg agcaaatcac tcactgcttg aaagaaaaca
    58861 agtatatcgt ttctcttata gtcaaaggag acaaaaaaaa cacgcttgat cttcctttgc
    58921 tgatgtataa gtggtatctc aagctgatac tctgtgattt ttgtttctac ttggcttttg
    58981 ataagacagc acaacaaagt ctcggcattt aacggaagca taactaggtc agaactagga
    59041 gagattagtt gctgaatgga atctatcttt ttatggattt ttatttgatt ttcttctttg
    59101 tagtaatgat caagcagttt tgttttcttt ttttcttcaa ctatggtcag cttttcacgc
    59161 aaaatctgat aaatctccca agattcaaac tcatctagaa cactaggtag ataaatcaaa
    59221 tcatcccact caaaaataag ctcttgctca gtagtcaaaa ttgcatcata ttccgttatt
    59281 ttgtttgatt gatttttgaa aagatacact ggttctatcc ggcattcttt gatccacatg
    59341 ttcatacttt gttgaatctg tttttttagt gtattcaaaa tactaattgg ctggttacta
    59401 acaatcagta tctccccttg ttttcgaaaa ggattcaacg cttcggcaag atacaaaaca
    59461 aaataagcgc tttcagctaa attgggacgt aattgaaaat gtattgggaa gaaacgccga
    59521 attaaataaa tttctaaagg ataacgggag atgatctttt gtacaaaatg attcatggta
    59581 ttatgtccgg catccatcct cgttttcatt tgtttgatat gggttagcaa aaattctgtt
    59641 tcatggaaca ataacgaaac aggaagatct aaaaaacgaa taatcgtttg ctcaaatgct
    59701 cttagattct tctctatttc tttgtttttt tcatcattaa tcatcaaata atttaactct
    59761 aatagtcgat tttcaattgc ttgtttttct tgattggaaa aagaataggg tagttctgat
    59821 gataactttt ttgtgagggt ctctaccatc ggaataaata atgtatacct gtttgttttt
    59881 tctaaaagtt cttctcttcg aagaatggtc aaaacgagat accgagaaaa tcgtatgaag
    59941 tcctctcctg aaacaatata attatatgat ataagaacct cttttaatgt cttttggaca
    60001 gtcggcatga tattggaaaa aattgtactg atttgttgag gcaaccttgc gtgttggatc
    60061 accgtttctg ttccaacaag agataagaaa attctttttg aattttcttc agcaatgatt
    60121 tttagtcctt ttgtcgacga aacttccagt tctaatttag gattgcgctc gatccaacgc
    60181 aaaatcgttt ttatttgtaa tgatacggct gtttttgaaa gaaagaaaat gtctgctatc
    60241 ttttgcatac tgacataccc tttttgaaac aataagtacg tcacaatagt tgaagctcgg
    60301 aaatccatgc tggaatagat tttatgtttc gtcacctctt gtatgatttg ctgtttagtt
    60361 tcagattcaa ttgattctaa aaaataacct gaacgtttat ttgactggat aatgattttc
    60421 ttctcgtaga tagcaagcat atttttgatt tcagtttgga tcgttttctt gttggtacct
    60481 aaagcttcag ctaattcttc gcttgttatc caatgattct tatttcttaa tactacaaga
    60541 atttggagtt gtcgtttatt taagtccatt tgcttacacc tcctctttgt tataaacata
    60601 caggaaaaac ctctcaaatg acaatagtca cttgcagagg tttaaaaatc atttgaagaa
    60661 agatctatat tcaaggtttt taccattaga ctggataatt tgtttataat attcaaaact
    60721 atcttttttc aatctcctaa agcttccgct cccatcattt tgtctatcca cataaataaa
    60781 gccatatcgc ttcctcattt cccctgttcc tgccgatacg atatcgattg gtccccacca
    60841 taagtaacca atgatttggc aaccatcttt tattgcctca gcaatttcct gaagatgtat
    60901 ttttagatac tcacaacgtt ttggatcgtg gattcgattc aattcatctg gtgcttcatc
    60961 tagtccaatg ccattttcaa ctatgaataa tggtaattca taacggtctg tcaattcatt
    61021 catgacataa cgaaacccta gaggatcaat tggccaactc caaggttcgg gagatttttc
    61081 cttcaaaaag ggattatcga aaccatttgc tccgccagta tctacgcgca tttctgcttc
    61141 ttttttatag acagcgctac gataataact aaatgcaata taatctgccg tgttagcttg
    61201 caagcactgt tcgtctccat ctaacattac tggtgcacag tcttcttcct tccatgtcct
    61261 gtaaatataa gctggataat gccctcggca catgacatcg gagaaaaacc atgaattgcg
    61321 tttaaattgc agtgaaccaa atacattctc tggatcacag tcatatgcat aagtcgctaa
    61381 tgcagaaaag ctacacataa tacccatctt agcatttgga tcgattttct ttaataattg
    61441 agtagcacgg gcgttcgcta gaaacatatg atgacaggct tgataaatca ctttttttgt
    61501 tagtgtagca atgggttcaa gtttatctct agcagggaat gtaaccaatc cagcagctgc
    61561 ataaggcata cgaaaagcat tattgatctc attaaaggtc atccaatatt tcactttccc
    61621 ttggtagcgt tcaaagacgg tctgcgcata aaattcaaaa aagtcgatta attttcgatt
    61681 ttgccacccc ccatactctc ctaccaatgc taagggggtt tcataatgac taagagtaat
    61741 tactggctcg atatcatatc gatttaatgt attgattagt ctgtcataaa atacgagacc
    61801 agcctcattt ggcttctgtt cgtctcctct tggaaatatt cgtgcccatg agatgcttgt
    61861 tcgaaaagct tttaaaccta actccttcag taaacgaata tcctcttcaa atcgatgata
    61921 aaaatcaatc gcttcatgag aaagataatg gagtgattcg tctaaagcaa tctcccaaat
    61981 ctttttttct tcattccact gaatactagg atgctggttc atgatcccga taagcgtatc
    62041 tgtcacatta ggtaatttgc cgtcttccaa ccatgcacct tcacattgat tagcagctac
    62101 tgctccgccc cataaaaaat tttctggaaa tatttcctga tccataacta tctctcctcg
    62161 ctatcaacaa tttcttactt gaagtatagc tcgattcatc aaaacaagat attctaacta
    62221 tttcggcaga agagaagaaa aaaattgaat gctactcttc atttttaata tttatatata
    62281 acaaatacat aagacgatcc ccgaaagaaa acactcgtta accatctaag caaacgattt
    62341 attttacttc caacacaaat gcttcataag gtcgtaaatc aactttgccg tctggtaact
    62401 gtttcagagg ataatttgac acaataaccc ttaaagcttc acgtggaaca gtgaacgtct
    62461 gcttttcact ggacatatta caaataacga gccattgctg gtcttcatat atgcgagaat
    62521 aggcaaagac atgctcaggc gtcttttcca ctaattcata gtctccccag acgatgatgt
    62581 catgttttct acgcaaatcg atcaatttct tgtacatata aaaaacagaa tcttgatctg
    62641 ctaacgcttc ttctgcattg atttctttat aatttggatt gactcgaagc catggaattc
    62701 cagtagtaaa cccagcatgt tcacgggcgt tccattgcat aggcgtccta gcattgtctc
    62761 ttcctttcgc attgattgat tccaagattt cctcttttgt aaatccagaa gaaatccttt
    62821 cgttatacat attgattgtc tcaatatctt ttgcttccgc gatatcatcg attgggcaat
    62881 tcgtcatgcc taactcttct ccttgataga tataaggggt gcctttcatc atatggagca
    62941 agatagcaaa catcttggca ctttcttttc gatacacatc gtcatttccc caacgagaaa
    63001 caattcgcgg aagatcgtga ttattccaaa ataaactatt ccatccttca tcacctaaag
    63061 atgtttgcca tttggataat acttttttca gttcagagat actcaagggt ttcaaatccc
    63121 atttgtcttt gccttcttgc tgatctaatc ctatgtgttc gaattggaag accatggata
    63181 attcatttct tttaggattg gaataaagtt tagcgatttc ggtagttgct ccccatgttt
    63241 cgccaacagt catcagattt ttgtcaccaa aagttgcttg attcatttcc tgaagatact
    63301 catgcagttt aggtccatta cttgtgattt ctttatccgg ttctttgcca atcatatcga
    63361 tcacgtccat acgaaagccc ccaatacctt tatcgatcca gaaattcatc atttgataaa
    63421 tctcttggcg aacctgttta tttttccaat tcaaatcagg ttgacgcttg ctatacaagt
    63481 gcagaaaata ttgccccgtt ttttcatcca actgccaagc agaaccactg aatattgagc
    63541 gcaatctatt aggctctttt ccattgaccg gatcacgcca aatataatag tcccgataag
    63601 gattttcttt atttttacgg gcctcaatga accacggatg ttcatcagaa gtatgattca
    63661 ccaccagatc catgatgata cggatatttc ttttctttgc ttcttcaatc agttcttcca
    63721 tttcttccat cgtaccgaat tctgccatga ttgcttgata attactgata tcatacccat
    63781 tgtcatcatt cggagactcg tatactggag aaagccaaat tgcatcaata cctaaatacg
    63841 ccagataatc caacctggaa ataatccccc gcaaatctcc aataccatct tgatttgaat
    63901 cttgaaaact tcttggatag atttgataaa caactgaatg gtgccaccat ttttgttcca
    63961 tgattttcct cctataaatg gatgtttcta ttttttgctt aatcatttat tttagtctct
    64021 ctcactaaaa attgtaacgc tttcaaatag ctattgcacc ttgttatcgg taacagaata
    64081 aaacaaaaaa acagctgagg aaattccctc aactgttttt tgaaaggatt gatttatgca
    64141 gggaagaatg ctgatttcac aacttcttta taaacggtgt tcactcagct gtcccttggt
    64201 attagctcaa aaaacggcaa aatatgagga actatttttt cgtttttttc tctaatactc
    64261 aggccagaac actgatttca caacctcttt ataaacggtg ttcactcagc tgtccctcgg
    64321 tattagctca aaaaacggca aaatataaga aactattttt tcgttttttt ctctaatact
    64381 caggccagaa cgctgatttc acaacctctt tatttcacat tcaatatcca catgaatccc
    64441 acaaatacaa agaacagacc atacatgatt ggagatactt cttttcctct tttagctgct
    64501 aacatggtaa ttggatagaa gataaaacct aatgcaatcc catctgaaat actataagtc
    64561 aaaggcatgc cgatcaagat caagaatgaa gggatcgcaa tctctaagtt tccccattcg
    64621 atttgtcgca aagattgtgc catcaatact cctaccataa tcaatgctgg tgcagttact
    64681 tgtgaagtaa caactgatag taatggagaa aagaacaagc ctaacaagaa gaaaatccct
    64741 gtagtaatag ctgtgatacc tgaacgtcct ccaacggcga taccagctga tgattctaca
    64801 aatgcaccaa caggagaagt accaaataag gaacccgcaa gcatagctgt tgaatcagaa
    64861 gctaaagctt tccctacacg aggcatcttg ttgtctttca tgaaacctgc ttggtttgct
    64921 aatccaacta aagttcctgc tgtatcaaag aaagtcacaa gcaagaatgt cagcactacg
    64981 acccacattt gaagagaatt gatgtcccct acatgtttta atgcaactaa aaatgttggt
    65041 ttcaaacttg gtgctgcggc aacaatttca gaaggcattg gaatcaaacc tgtgaccaga
    65101 cctaaaatcg ttgtagctgc tatcccgatg aagatgccac ctggtacacg ccgaacaagc
    65161 aagatagcag ttacgactaa tccaaaaatc gtcaaccaag tagaaccaac gttgagtgat
    65221 cctaatccta ctaatgtaga ttcattcgaa acgatgatcc caccttcgct cagaccaagg
    65281 aaagcgataa atagaccgat accgccagaa atcgcatatt tcaaatctgc tgggatcgca
    65341 tcaatgatca tttcccgcag cttgaagatt gtgatcaaaa taaaaatcaa agaagcaacg
    65401 aatacgccgg ctaatgctgt ttcccatgga atccccattc ctaaacaaac agaataagca
    65461 aaaaaagcat tgatcccaag agcaggtgcc gttccaattg gataacgggc taataccccc
    65521 attaaaacac agccgatcgc actggctaat gcagtcgcag taaacactgc tccttcatcc
    65581 attccagaag cacctaatac agttggattt acaaataaga tataagccat tgagataaat
    65641 gttgtaaatc ctgccagtac ctcacgtttt accgtggtgt tcaatttttc tatttcgaag
    65701 taggaaagta ttttttcctt catcttccat ccccctaaat ccgaacatta taaataaagt
    65761 ccgttaaaat ttcgatttat taataaaaca acgtgtttta tcatatacta aaacaaaaca
    65821 taagtaaaca aaaaattcgg attttaagca aaatccgaac gttccatctg attaaatccc
    65881 tttatctcaa agttcatgaa cattcacttt ggattacaag aatttcttta ttgtgttaat
    65941 aaagtttctt cattctagtc tcaaaaatca atagtgtgat ttgattttac catttcgttt
    66001 ctgttcttct ttgatgaatt cttttgtatc gacaggattc agattattta gtccgccaaa
    66061 acctgtatcg accatttttc cccagccttt tgtcttcttt tttcctgatt tgagattctt
    66121 tgctcgaagc ttctcactat gttttgctcg ccgatacttc aaataacctt ctaatcttcc
    66181 cattagatcc actgtttttc ctccttttta ttcaatcaga cgactcagct tttcctataa
    66241 caaaataatt gccttcaggg tcagaaaaat tgaaagtcaa gatgccattg ttatctgtga
    66301 tttctcctgg ttcttccatg ttctcgtaga tagtttcaaa ttcatccgta aaaaatacta
    66361 aagaaggcgg tggtcctaat acctctggtg aaaacttctg cacaaatact tttgggaata
    66421 cgcctaattc taattctttt gagatgctga gtacaatact cttaaattct tctggcaatt
    66481 cgattgtttc agaaatttct gcaccaattt tttctacaaa gaatcggcaa atcatctcta
    66541 cgtcattgac ataaagcatc acacgtgttt tttgtatcat catttgaccc ttctttctat
    66601 ttgattttct ttcctgtcta taaactgatt ccattatatc acgaaagcgc aaacaccttg
    66661 tgtttgatac tattcaagaa aaataaagca atagtttcta ggattgcttt cctccacaaa
    66721 ctttttttac aaaaaaaaaa aacggcaatg aatgccgctc ttcttatttc gattctttta
    66781 taattctgct tctgattcat ctttggttag tgtgtgtcta acactagtta cgccaaatgt
    66841 ttgatcgatt ttttccttca acgcttcgat tgcctgtgcg ccacgtgccg cttgttcagc
    66901 attgatggat tcaatgacca gcaccgctgc ttgtgtttct tccgtcagca ttgttctttg
    66961 ggcttcacgc cagttgagac agcgacagat cgctccttgt tcatcatagt agatgatttc
    67021 ttccggaagt gccggagcat cggaatctgc acctaaaggc aggaatgatt cgccaccttg
    67081 ggcttttcct aaatgcagat ttcctgcgat cttttcgata tcttctccgc cgcaaggtac
    67141 gccaaaagac aaggaaatac tattatagat atctacaagc ggattgattg ggaaaaattc
    67201 ccgttcttga tttgcacgtt tcagtaaagc ttcaatagaa gaacgtgcac cttttttagt
    67261 cttgaatgtg gtaaatgcct gacgccactg agcaatgact tcgttttggc tgaatgtttc
    67321 ttctgtaagg taatttcttg cttcttttgc tgcctcattc aataattgat gatacgtttc
    67381 ttcactttct tttgttacgt ggttatcgat accatttacg gataagattt cgatggttgc
    67441 ttcaggaaat acttcccaga aattggtatc cactattact tttttcatct gttcacacct
    67501 cttgattaga ataattgctt gatgaataga tagtctgttt ggggatcatc tcccatataa
    67561 aaaacatgtt cgctgtattt gataaatcct tttttacgat aaaaagcgag cgcaacatcg
    67621 ttgtgttccc aaacaccgag ccaaatggct gttttttgct tttcttttgc tttttcaata
    67681 gctgtatcta gtaaaaatgt accgatacct tgatgtttaa atttttttcg tacatagatt
    67741 cgctcgattt ctaaaaaatg atcagccatt tcctcagatt gactcgttcc tgtatttagt
    67801 ttcaggtaac ccgcaggctc ttgatcgata ctcactagga aaaaatcggt ttctttatct
    67861 tttagctctt ctgtaagttt ttctagtgca taagcttgtg tcaagtaatc agctaaatgc
    67921 tccggtgaat tatcttttcc aaatgtatct gtaaatgttt cgatactaat ttgttgtagt
    67981 aagtctacat ctgaaggcgt catttttcga atttcgtatt tattcacggc ttcttccttt
    68041 caatagatcc ttttattgcc ttttttgaca aaattccaat cttcgctaac attcttgcat
    68101 actttattta aaagatattc caattgtttt gcttcttcgt cagaaagacc atttaatgcg
    68161 actgcattgg agtagttgtt ttctcgaatg atgaaaggat agatttctgc tcctttttta
    68221 gtcggataga gtttcttgat ttttttattt tctttatctt ccaaacgttc gatcatccca
    68281 ttggattcta atttcttgat tgctcgggca gtcgttgtac gatctacttt gatcatttct
    68341 gctagttttt cttggatgat tccaggattt tcgcaaatac gtactaaata aagatactgt
    68401 cctcttgtta aatcgacttc tttgaattcg atattgctaa tagaatctag cgctcgggca
    68461 atcattccga tgtctcgaag gatttcagcc atttcatccc acctttattt tgtttgtaag
    68521 actagtttac ctctttttgt tgcaaatgca actattttca ataaaaaaat caagttcttt
    68581 tttaataact agtaaagtat attcttttct tcttttacga aaaagctcaa tcacaataga
    68641 tctggttttt gttttctaaa ataagtaact gtttcttcgt tgttcgcttt tctgatagct
    68701 ttggcaactc ttctatacta aaaaatccca tgttggaagt ttcattattc tcaataaact
    68761 tggcctcttc accgtggatc gcacaagcaa aaacaagttt ataatactga aaaagctgtg
    68821 gaatatcttt ttgtttattc gtatcaaaaa ctgctcgcaa ctgagtagtt tctactgtta
    68881 atcctgtttc ttcatagact tctttacgca cattttcttc aggagacaat cctatttcag
    68941 caaatccacc aggaagtgac cattctttcg tccgtttatc ttcgattagt aggaccttgc
    69001 catctttttt gatataggca cgaacatcta cttttggtgt tggatgtcct tctccaatcg
    69061 ttgtgagcgc ttttatttct tcgaaagttt cttttcctat ttcactgatc agttccaatg
    69121 aaatcgtccg gatctcctca aaacgctctt tatcaaatac atcttttcca taatatagac
    69181 cagcttgaga aagtgcgagt agtcttttgt attgagccaa cttcttgttc attctcttcc
    69241 cttctttttt agcaaatgta tgactatgta atcaagtata gaagactggt ttaattacgg
    69301 caaataaata taatgaaatc tgcaataaat aaaagaatgt taaaaaactc gagaacaaaa
    69361 gacattcgac ttctctgggg ctgtgaataa atggcgttca aaagcttgca tctgcggtaa
    69421 taagccccac caaacgaaaa atatgacgag ctattcttgt ctggctgttc ttattaattg
    69481 aggcaggaca gctttttcac aacctcccca ctaaacggtg ttctcctacc aactcctcgg
    69541 tcacaagtca caatattcat caaacatgag aagctgtttt tttgaaattg ctccttgtga
    69601 ctcggagctg aacgacaggc tcacgacctc tcggtaaaca gcgttcaaaa gccaattttt
    69661 cgatattaag ccatttcttt gttacttatt gtcattgatt tctttttcta atttatccaa
    69721 tagtggagtg taatccactt gagtatagtc tgttacatca aattcatata gtgttcctaa
    69781 ctggaatttg tcatattttc ggtcggcgat gatctttttg aatgcttctt tcgtaatatg
    69841 attggtccct ttcgttcgat cttccaatac gtcaccatag tgataatgat ccatgatata
    69901 gctcaaatga cgatctgatt cacgatcacg tttgtaccga cgttgacata acacttcaaa
    69961 gtctgctgtt aggcgaatcg tgatcacctg atatccgtat ctatctgcta attcttggag
    70021 tcgtgctttt tgcttatcac taaaaggata ctcggacaca atcactttct ttcctgcttc
    70081 catatacaaa tcaagtgctt tgtaatagaa aggataaact ttcgtccttt cttgttccgc
    70141 ccgttcttcc aaattgttaa acccgatgga ttctgcatag tattccttga tttcatcgag
    70201 cgtgatggca tacatatctg gaaatcgttc ccgtagttta ttcataagat atgtttttcc
    70261 tgtaccagga ctaccagcca gaagaatgaa atactttttc atctcattta tttcctaagt
    70321 taattggact ttccaagttc ataaacagtc caatcttatg cttgacaatt tcttttactg
    70381 cttcatttgc tggaatcaca ttatggcgaa gagacttgtt gatttctgtt tcactaaagg
    70441 tttctttagc tactttcgtc cagttgacta aaagctccgt tccaacattg aatttgcgga
    70501 tcccgttatt gatcaattca ggataatctt cttctttcac acctgttcct ccatggataa
    70561 ctagggggac atcaactact gcatcgattt ctttcagcaa gggaatgttt acttctgttt
    70621 ttgatttgaa ttgtccatga ttcgtaccga tagcaatagc taatgcatcc acgcctgtag
    70681 cttctacaaa tttcactgcg tcttccggtc ttgtatatac cttgtcattt tcagcgacat
    70741 ggattccttc ttctgtaccg ccgattgttc caagttctcc ttctacggaa acaccgtgcg
    70801 catgggcata ttctactact gcttttgtct tcaggatatt ttctttgaaa ggcagatggg
    70861 acccatcata catgacagaa gtatatccgc tgtcgattgc ttctttgata tcatcaaagt
    70921 ctcgggcgtg gtctaagtgc aagactgcat caaccatttc ttcttctgca atagctgtca
    70981 ttacattgac taacacatgc atgccgatat atttagcggt atcgacactt gtttgaataa
    71041 tgatcggcga acccatttct tttgctgcgc ctagcatagc aggaagcatt tctaggttat
    71101 gcgtattaaa agctccaact gtaaaattca actcttctgc tgtttttgtt acttctttca
    71161 atgttgtata catcttttat tgccacctta tctttttatt tgtttccttt gatcgttact
    71221 tgttttgcaa tttgtcgcat ctcatccatt ttgcccatgt agtaatcgat cccttgatca
    71281 tcaccagcac gagcagcatc tgcacgcttc tggttataga ggctcatcag ttttttatac
    71341 ccatctccga tcgtctgttt attttccaaa ctcttttcta aatattggat ggcttgatcg
    71401 ttcacataca attcagaaaa tttaagtcca acttgttcat aaagtttcgc taatttatgc
    71461 ttatcttggg tagtggaaat ctcttgtttc aattgtgtaa tctctgattg aatcttttcg
    71521 gtcgcttctg gcgatagggc tttttcttct tttgccagaa cttttttcgg ttctttttct
    71581 tttttcttaa aaaatccaaa catcattttc cctactttca attaaaatgc tttcaaaact
    71641 gggcttaaga tccaggcata taagagtgct gcggctactg tatctacatc ggttgctgta
    71701 gcattgatga atcccatcga attaaagatc gttactagca aagcgggcaa taatgtaata
    71761 aagaaaccat gagcaatacc gccaatgatc gctcctcggc gtccgccaac tgcgttgcca
    71821 aatatccctg ctgtccctcc ggcaaagaaa ttggttagca taccaggtaa aatcatcgct
    71881 aagccgaaca tgggcaaagt aaacatggca ataatcgtac caatcgttgt cgtaatgaaa
    71941 cctaaaatga cagcatttgg gctataagga aagaaaactg gacaatctaa ggcagggatc
    72001 gcatcaggaa ctaatttcat agctatccct cgaaatgccg gcacgatttc acctaacagt
    72061 agacgtacac cagctagcaa tacatacaca cccactacga attggatggc ttgtaaaaag
    72121 gcaaacatga tatagttttg atctcctgat aattcagaag cgaatggttc gcctgcaaat
    72181 aaaacagtga tgatatatag cgggaccatc acgaccatga cagacaaata agtatcttgc
    72241 agaaattcga aatgtgtcgg caacttgata tcttcgatcg actttttatt ttcgcctttc
    72301 tcaccaaaaa gcttagcaac accggcttca aataagtacc cgatcgtaca aaaatgacct
    72361 aaagcaatat catttgatcc agtgatttta cgaatgatcg gttgcgctac tgccggcata
    72421 gcaatggcga aacaagctcc gataaaacca ccgaccaaga tcaatacgat ccctcgcaga
    72481 ccggcaaaat agccaaagac tgtcgtcatc gttgccatcc ataaaattgc ttgaccagtt
    72541 aaaaaaatgt acttccattt tgtgaaacga gccaaaataa tattgaatac aaaaatggct
    72601 aagaacgtca gtgcaatatc tctacctaaa cccaaatcat tcattgcttg accattgata
    72661 gattcaatag aagggatgat cccttgcata tgaaatcctt cagtaaagat tttcccgaaa
    72721 taagttaaac ttccaacgat gatgctcgaa ccggcactca acacttgaaa tcctaggagt
    72781 gtctttaacg tgccagagat gacttgtcct gctgatttct tctgcagtag tagacccaac
    72841 atagcaatca atgcaatggt gatcgatgcc tgagtcaaaa tattttcgat gataaaattg
    72901 ataacgccca tgtttttacc ctcttttctt tttgtattta gataactccc ttatcttgca
    72961 gaactggggt gattttttct tcaatttcat tttttgaaac aatatttttc aaatagatca
    73021 tcgccgtttt aggatcaact gcgaactttt cgaactgcga acgaaaattt tctgctgtga
    73081 tgatcacatc tggtttcatg gaaactgcgg aagaaatatc acaatggtcc agtttggctt
    73141 ctactcctaa tttgttcaaa acatcctcag cagacatctg tgctgcaaaa ctacttccta
    73201 aacctgcacc gcatacgaat aaaatgttca atttgttcat ctttgttctc ccccgtatca
    73261 tttattattt ctcaactact gaaaaaagca gttgtttgaa atcttcgata tttgtgcatt
    73321 caatcaatcg atccaacttc tcttcgttgt tgatcaattc aattagttct ttcatgatgt
    73381 tcagatgcga gtaggagtct actgctgcca aacaaaaaat aatctttact ggatccattt
    73441 caggattacc gaaatcgatt ggttgctcaa tcgtagctac gctgactcct aatttattga
    73501 ccccatcttc tggtcgggca tgggctagtg ccatgtgttt gccaatcacg atatacggtc
    73561 cgtaatcatt cactgcacga atcatcgctt cgacatagcg ctcttcgaca atgttttctt
    73621 taaccaatgg tctagcagct agttcaatcg attcctccca agtagtggct ttttctttga
    73681 tcatgatatt gctgtccgtc aaaatatctt ttaacatagg ctggatctcc cttttgttga
    73741 tttctaaatt gtttgtttcg aataattttt ctaacttgcc atagatgatt cgatctactc
    73801 ggccaccact ttctttcatg acatctatta tttgagtgaa taactccgtc gattcatgac
    73861 tgttaggctc taaccgctgt agttcttcat ttcttttcaa aaagtcttct ataaccaaga
    73921 tattcgtctc agttaaaatc ggatcgacaa ctaaaagcgg cttatttcga taattcaatt
    73981 gaaaggtgct gaaaaccaga ttgacatcca atttatctac taattcgatt tcttttgaac
    74041 ttaaaactgc agtaatctcg atctgaggaa acttttcttt cagattctct gctaacagat
    74101 ttccagtagc catcccatgg ttacagacaa cgactgattt ataaacataa ttcaagtctc
    74161 gcttgattac actggcgatc gtcgaaaagt ggatgacaag aaaagctatc tcgtcctcca
    74221 gtatcttgcc accaattact tcttcgatcg taggaacaaa atgagtgact gcgcggtaga
    74281 ttgggaaata atttgttcgg atgttttctt tcaatggatt catgacttgg atatgattct
    74341 tgattcttac aatcagtgca gccatgtgct tatagagatt ttcgaataaa gcttcttcct
    74401 ttagatggaa aggtacgtgt gtctcgttct ctacaaattg gatcaattgg atcgttagta
    74461 attgagcttg tacccactcg attgaattgg agatatctct agcattgaac gtctcaatag
    74521 tgaaacgaag ataatttctt tctacttgcg ggacattgtc caattgaaat tcctcgatca
    74581 tgtggtcaat aaatgcagaa aagctactgt cttttatttc tgtatttttc caattaatgg
    74641 cagagataaa ttcatgtttc ctgattcgct ggatccagat caatgtaaaa agcaggattt
    74701 gttttttata aatatcatct tcttgtttca acaacttatt ggaatagatc tcctgtattt
    74761 ttttgatttc caccaaagga aaataatgga gaaagatttt ttgagccggt gttaattgtg
    74821 catcttgatt cataaagaag tcggtcattc ccagattctg attgataaaa tcatagatca
    74881 tcgtacggat agatcgttct gaacctttat aaaccagtcc ttgctttcca taactgacga
    74941 tctctatccc ataactgacc agtgttgctc gtaaccgtct catatcttca tctaatgtac
    75001 ttttagagat ttgaaattct tcttcttttt tgtttagatg gatcgggatt cggctgtaag
    75061 caacggctaa aactagatca aaaatacgtt cgcgccgatt caaaaagccg tctttcacat
    75121 cttttatttc agtttctaat cgagcttgct tttccttttc cagcttcaac aaaaaaactt
    75181 tgttgcgaat tgtacggatc tccggaagct ttttactgct caaccagata ttcgcttcaa
    75241 gaacttcctg acggatcgtc cgctgactga cattgaactc acttgctaga tgttttgctt
    75301 ttactggata ttgggctgtc atgattctgt gaagtatttc aattgttctt gttttcatag
    75361 aaatgatctc cttctgattt caagtatatg gttccgctta cataataaaa agtagatatt
    75421 tctgccactt gaagtggaaa gaaagtaagc aaagcaaaca aaaaataagc catatgcttt
    75481 ttcatctcct caacgaaaaa gacatacgcc tattattaca aaaattttgc ccatctatac
    75541 aaaaaagagg ttgtgaaaaa gctgtcctgc atcaagtaat aagaacaact aaactaaaat
    75601 agtgcttcct atttttcgtt tggttgttct tattatcgca gatgcaagct tttaaacacc
    75661 atttagtgga gaggttgtga cagagatgtt cagccttgag cgataagaag cgattttgaa
    75721 aaatcgcttc ccatatttta caaaatcgtg ccttattgcc gataggctat ctctggcaca
    75781 ctgtttattc agaaaaagga gctgcgacaa ggcttttgtc acaactccgt tcataaatat
    75841 ttcctgtcaa tccagcataa tcggctgaat aaaatcggct tgccatcttt tttctttctc
    75901 agaaaaatca aaataccata tctgagaaaa atactgtcgt tgtgtcttac ttcctgagat
    75961 caatcgccca tcttttcgat cgactgaata atcacagcat ctgaaatcaa ttcggatggc
    76021 aaaactattt tcgtggatct gtcgataatt tcctacttcc tgaatagtta tttttttagt
    76081 atgatttctt acaccgtctg ctttgttttt ttgcagaact ttctgatgtt cggcaaacag
    76141 cttgtctgta taacagcttc tggctttttc aggagtttct tcttcccaag cggattggat
    76201 cttcatgaat atcttttgga tttccagaaa ctgtgctttc ttttctttag ccgttcctga
    76261 catgcgggac aatatctccg ctttcagcct tttcttttgt cttttattct tgacgataga
    76321 aaaagcagaa acccctccta ctgacaaaac agctaatgca agaaatcccg tttgctcata
    76381 actagaagga cctgtacctg gaccataata cccataagaa tctctgccgt aagaacgact
    76441 gccaaaataa ctgcccccat atgaacgacc accaaatgaa cctctgcccg aacttcgacc
    76501 gctagatgta cctccaccat gaccacccgc gacagcgaat ccttgggtcg gcagagcaaa
    76561 caaacatagg cccactaaca aaatgattgc taaataattg atttttttca ttgtcttcct
    76621 cgttatcttt tattacaaaa aactattttt atgatagcct tgctaaaagg aagagtcaat
    76681 aaccagatgc ataaactgtt tttttagcaa ttttgtctta tttagtcatt gagtaacatc
    76741 ggacaaatgt atgtgtcggc agtccataat tcctctatct tcagcatgaa caagcttgca
    76801 gacaagagat ctgcgcttcc tccaggagag atttggtttt cctgacaaaa gtgcaacagt
    76861 gctgtaaatg gacgatttgg taaagcactt tgactagcag catgagcaag tttttgcatt
    76921 tccactaacg ttttctgatc actgcgatgc aatacgcatg tgtcatctag cgaactcatc
    76981 aaaactaaca acatatgcat ttgtttctgt acatatgaat cattatctag ctccttagct
    77041 gcttggattg caagcctgat atgcggatac cctaatatcg cttcttcata tgcaccattt
    77101 acgccatatt tttgcttcac cctatgacca tgagtggttt ttttatgacg atattttgta
    77161 tcaggaaaag cagcaagctt gctgacatca tcaagtattt tcgataaaga aaaaggctgt
    77221 tccatactga ttttttgtgc aatgacactg atcaatagcc ccattgccca aatcgctcct
    77281 ttatgggtat tgatgccatt tgtagcaatc agcattgcct tttccgcttt tcgcccaatc
    77341 tcagcaattc tttctcttaa ctcctgatca atagcatgat gccatgctat ttgagccatt
    77401 tcttcaaaaa aaggagttag cgtcttagca gacttaagaa ataaagcata gtccatatcg
    77461 gaatgtgcac ccgcatccag tgcatccact aatccaggtt ttggacttaa tgttacttct
    77521 gcgatcaatg cttcctctac caacgcagcg agttgctgag tcactgcttt ttttcgttcg
    77581 tcatttatca tgacactcac tccttcttcc tactattact tgctgaaacc ttttttgata
    77641 gctacccaaa tgatatgaag taaccagctt aaagcaaatg cacaacttgc cgtcattaga
    77701 taagctacaa gaatcatgac tcctgaaggt aactgcagcc aactatcttt aaacaataat
    77761 aacagtactt ggaaaacaaa tacattggct aaatacgtac gataagcata aacagacaac
    77821 catttgaaca atggcaattt tggcgaatcg tttacaatca ttttggaagc gcctatgaag
    77881 acagcaaaga tgatgactaa actgtataaa ctttggatcg tattcaagta aggtgcattc
    77941 gcaaagctca agtctcctgg atgtgcgagc aatgaacgga cagaaaagaa cgcgataact
    78001 aaggcaacag gtaagaaaag ataacgtact ctgtccaaat attgatagac tttttcgtga
    78061 taaacaaatg cggctacccc ataaatcatg aaactagcaa caaaccgatc taataagtac
    78121 cagctgtcat gataaggccc agtcaatact tgtgtttgat agaacagata ccatgcgaca
    78181 tataaaatcg ttgaaccaat caatactggc catacatatt ttttattagc aaatactttg
    78241 taaccgagcc aaacaaaaaa cggcatgaac aattggatct gcagcatcat aacggtatac
    78301 cataaatgag gcgctccatc acctgtgaca tatttcagta aaaagcttgc agtatccgta
    78361 tacggagttt gttgttggac tgctggaaat agccaaagat aagccgtcgt ccatagaaga
    78421 tacggaacga ctagttcaaa aaacttttct ttcaaaaatt cccaataaga caaatgctga
    78481 cttgttttta ccatattata aacaattgca aaaataaaca ttggtgctgt gtatttaacg
    78541 atgacgtaaa aggtaccaag aaaagctgta tttgttgcgc tgtggctgtt tgccaaagca
    78601 aagctcaata tcgtttgcat cataacagcc gttacagctg atactttctg gaagtcactt
    78661 acgttgattt tgattttttt gttttcaaac atctctttct ctctcttttt ctttattacc
    78721 aaagttcttt ggttaattta ggtccttcga ctgttttcaa tagaattttt ttctctggct
    78781 ctctcaaata ctcttttagt gaaaaaccgc cttgccgagt cactacttgg gtatcgaatt
    78841 gctcaaaaaa agcaggatgc gattgaatta cctgaattgg caattcaatc ggtgaatttg
    78901 cgtacagcag caaatcaaaa tcactggttt cttttactgc cggaatccca gtcgataatt
    78961 caaatcctaa gctgccgccg attccccatt tacatcctga taatatgttt ctagcagcat
    79021 gaaattgctg ataaacagga taatccatga ttttctcttg tctatacgat tcttgttcga
    79081 tgatgtccca cggatgaatg acttgtttga cggcatgttt tgagatttct gttgaccatc
    79141 tctgattgcg ggcaggtcca cgtaaaccaa tcggtagtat ttctgcgtga tccgtcgacg
    79201 cacgacgaac tgttccgaac atcttttcag ttagaacgac ccattcaggc aaggtgaacc
    79261 cttctatttc atgtttatca aaaaggacaa tatcatgtgc ttgaatcatt ttttatgcat
    79321 cccattcttc gtccattttg gcacggactt tatttgtttc gacacgtccg aatgttttgg
    79381 ctgtttcgtt tgtgtatcga tttgtcagca tcgtgtcatt tgtattgcgg acatcttgaa
    79441 tggcttcatt gattgcttcg atgactttgc tgacattttg ttctgaagca gcttggtcgg
    79501 tgatatcttc taggaaatga tagatcgctc ctagttttgt ataattattg atatcgtaag
    79561 cgatagctgg aactttttca gcagctgctt cgatttcagc aaccgaacgt tttgtcacac
    79621 gtgcagcact ttctttagac atcgcttgga cggtaatttc tttattgttt aaagcaatca
    79681 agcggcttga ttgtaatcca tgtgctaaaa atgcacctga aatagcattt cctgcaatca
    79741 atccaataac tggatgtcca gcttgacgga gttttgcata agcagcagca ctattagcca
    79801 aagccacatg gataccgatc aattcttcgt tatagccgta tgcttggctt ggtacatcga
    79861 tcacaacgat gattggccgt ttcacttttt tgtctttatc ttcttcgtaa acgtgatcca
    79921 aaatttgtcc catgcggaaa ccttcaacta aaccaacttc accgtttcgt acacggtaca
    79981 tcgggttttt aggatcaggt acgattgatg tcacgatggc ttcttctatg aatttacttg
    80041 atttaccata atacacagta ctaatagaag aagttggatt tttcatgcca gttagtgccg
    80101 caaaccaatc gtaaccaaca cttgctgcag ctggttcatt tccttctgct acttctggta
    80161 agtcaattga ttttgcttga tggttagcaa ttgcttcatt gtaatcgtca atggccattg
    80221 gttttgaaag atctaaagaa tcaagtaatg ataaataaaa tgctgagctt tcactgcgat
    80281 gcgtgtcttt tcgttcgtcc attgctgcga tgacagcagc tttgattgct tctacactat
    80341 cagtcactaa ttcatcaatg atttttgatt ctacccgttg acgagatcct aatgtattcc
    80401 aaatcaagtc tttgtcacta gaatcaaatt ctcgaacgcc tgcttcttgt tcaataactt
    80461 ctggtccatt caaaccgact ctcgcttttt ttgttgcgat taaataactc attaatgctg
    80521 aagtaatcga cataccgcca aatgatccta cacgtcctgg aatcaaaccg atgactggta
    80581 catattgttt tagagcaatg attacatttc cgatttctga aatagataac agtccgtagt
    80641 ttgcttcttg caaacgaaca ccacctgtat ctagtacaat gactggaaag atctcatttc
    80701 cttttttatt ttcttctaac acatgttcaa gtgcagcaac gatttttgca ccggaaactt
    80761 caccgatacc gccaccttgg aattttcctt ccatagcgat tactactgct tgtttgccat
    80821 tgatcgtgcc acggctgaca acgatcccgt catcactttc agggacgatt ccttgtgctg
    80881 gtaaacttgg cgcgatgatt tgatcgaatg gatcaatcaa ttcacgtcca ttgtcaagta
    80941 gagcgttcac tcgttctctg gcgtttaatt ctacaaaact atttttcatc tgaaagttcc
    81001 tccattgctt gcgtcaatcg taggtacaca actcctggtg tagcaccgaa gtcattgatc
    81061 acgatatctg ctgaaattgg ataacgatca aaaaatctcg ttaatacatt gttccatact
    81121 tcgccgaagc catcgcttcc tgtacaaatt tttacagtag attctgtttt ttctgttgga
    81181 aacatcaaga tttccaaatc tcctgaaccg accactccta catggatttt cttattgatc
    81241 ggctgtttcg ttggatattt ataagctaat tgttccatgg atctctcccc tttttgtaaa
    81301 acagagcata cacgttctgc ttattcgtct ttcttcttcc atctgggtaa atggcgacaa
    81361 aaggtttttc tcgccaattt tatgaccaag ttctgaattt cattggtggt tcatacaggc
    81421 catctgacca ttcgatcaaa tcatcgatgg tttgtgcagc taataatgaa cgtttcgctt
    81481 cactacgttt gatgcctaga tcttctggta atttcacgat gccggctgca cgcaagctat
    81541 ctagtgattc tttcgtgcta gtcattccaa gaggtgtaac accgccaatt gctgcaattg
    81601 ctgcttgtcg ttcttctttt gtttttgctt tgtacaaata agcaattcct tcttctgtca
    81661 cgatatgtgt agtatcttcg ctataaatca tcactggtgc attggctaag cctgcttgtt
    81721 tttgaacagc tagtgcatct aattcttcga caaagactgg ttttttattt gaaccatatg
    81781 tttcaaccat ttgaacaact aatttttggc ctttccctaa tggatcatca ttgtcgcgaa
    81841 gtgattgcca tgctggtgta gaatgacgac gaccgccagg attatgtccc atgttaggcg
    81901 caccgccaaa accagataaa cgtccttttg ttacggtaga agagtttcct tcgtaatcta
    81961 attgcaaggt tgaaccgata aacatatcga tggcatattg tcctgcagct tgtgataatg
    82021 ttcggttact gcgcatcgtg ccgtctttac cgacaaaaaa gacatctggg cgagcagcaa
    82081 tatatttttc cataccaact tcgccaccaa aactatggat gctttcaacc caacctgatt
    82141 cgatggctgg aatcatcgtt ggatgtgggt tcaataccca gttcttcgcg attttccctt
    82201 ttaaccctag tgattcacca taagttggca gtaacaattc gatagctgct gtattgaatc
    82261 caataccgtg attcaaagat tgtacgccgt gttctgcgta gattcctttg attgccatca
    82321 ttgccattag aatttgcaat tctgtgatgt tttgaggatc tcgtgtgaat aaagcttcta
    82381 attgataagg tttatctgct ttcacgacaa cgtctaccca gtcaccaggg atatctacac
    82441 gtggtaaaga atcgacaact tcgttgactt gaacgatcac gataccatct ttgaaagctg
    82501 cagcttcgat gattgttggt gtttcttccg tattgaaacc tgtgtacaag ttgccgtctt
    82561 tatctgcttt atctgcagct actaaaacaa catttggaat caaatcgatg aataagcgtc
    82621 cataaagttc caaataagta tggatatcac ctaatttcat tgtgccgtct tcaatcattt
    82681 gggcgacacg caagctttgt gcaccagcat aagagaaatc gatcttgctt gcgattcctt
    82741 tttcaaaaat attcaaatgt tctggtcgtg agatactaga catgatcaaa tgcagatcat
    82801 tcactttttc tgcatctact ttttcaagac tttctgaaag aaaacttgct tgtttttgat
    82861 tgtcgccttc taatacaact ttgtcgccgg gagcaatcag tttttccatt acttctacga
    82921 ttttttcagt ttctacatac ttgccattga gcaagttgct gattttttcc aatcgctgcg
    82981 cttttttttc gcgtttagaa gcccagttgc ggtttggttt ttccataatt agtcatctcc
    83041 atttttgata tagaaacgtg gcgtcatcaa taccatgttg gtctagattg atcgtacgga
    83101 tccctttatc tacaccaaat tttgtatgaa tcagctttgt caaagtattg ccggggggaa
    83161 attcaatcgt cagatccatt cctaattctt ttgccaagtc catcatttgg ttccactgca
    83221 cagggaaagc taggttttcc aacaaatctt tacgaatctc ttctgcgtcc tgagtggctc
    83281 ttccatccgt attttttata tacaaacatc tagcatcaga tagatggaca gactgcatat
    83341 acggcttgaa tttcttgacc gtatcattca tcaagagaca gtgagatggc acagggactt
    83401 tcagcagctt cgctgtttgc gcttggtgcc gtttggcttc tgccaacgta acggataaac
    83461 cttcgatact cccagataac gtatgttgca tcggacaatt ttcatttgac agataaactg
    83521 gatggtcagg atcaaatgtt tttgctacaa tttcctcagc ttcgcttctc gtcacaccta
    83581 ctactacgcc cattgcataa ccgcttggat aagcttcttt cattaagact gcgcggtgat
    83641 agactagttc cgctgcattc tcaaatctca aagatcgaca agcaacagct gcacaaaaag
    83701 caccaatcga atgtcctgct gcaatatcag gctgaatacc gtttttttcc atttcatcta
    83761 aataaaatac tgcttttgtc agtaatgcta gctggataaa aaccgtatct tgatagtttt
    83821 cttctttatc aactaaccga taccccgtca cattttctaa acgttccatg tacatttcag
    83881 ggatatccgc taacatccct aacttctgac tgccttgtcc aggaaataag aaacctgtac
    83941 tcatgagcct cgcctcctca gcacttactt tttccactgc tatcttagtc aatttctgct
    84001 ataataaaaa atgccaaaaa aagaaaaaac cataacaaaa aggtatggat gggatggata
    84061 attatgaaat tgcttcatct gcagtatttt aaaaaagtat tcgaaacaaa aaatgttacc
    84121 caagcagcca aagaattatt tatttctcaa cctgccttga gccgtgcgat caaacatttg
    84181 gaaaacgaat taggtgtccc tttgttttac catagcggca gaaacattga gacgaccgtt
    84241 tatgcagaag aattttatcc atatgcagtc aagacattag acacattaga agaaggaatc
    84301 aaattattgt cttcggtcaa tgagaagatc gttacttccg tcatattgta tttagaggta
    84361 gcctctgttt ctattccgaa cttggtccga atttttatgg aaaagcatcc tgatatccaa
    84421 ttaacgatca tgcaacatca tttaccagat gatattaccg atcctgtact gtacatcact
    84481 tcggagatga agcctggcat gaccaatatt cctatcatga aagaacctgt ttatgtcgcc
    84541 attcctaaga aacatcctct tgctaaaaaa gaacatctgc aattagaaga tatccaacgc
    84601 acccccatct tgatgctgtc aaagagcaac gccttccgtc atacactgga tgcagcactt
    84661 gaagcgaaaa gtatcgaatt gacgatcagt tcgacgacag atgatcctgc gacattacgt
    84721 tctattgtca agcaaggatt agctgttagc tttttcccaa aagtttcttg gtcttacgat
    84781 aaaaatgatc ctttcgtctt gcgggaaatc aaagatttgc ccttgacacg aacaatttat
    84841 ttatcttcta gcttcacaga agatcatcct ttgatcaaaa tgattgcaaa aacgatgcgg
    84901 gaattttatt taggaaaata aaaaaaaaag agctgggaca aaattcttgt cgcagctctt
    84961 ttttcgaaca aacggtgttc cagaagtaat ttcttagaaa ataagccgaa atttcccaaa
    85021 aatttgagaa gcaattttcg taaattcctt cttatttctt gaagctaaac atttctgtca
    85081 caacctcttc tctattgtcc tagacattca ttgcgttgat ttaaaacttc catcaatcaa
    85141 atctgctagt tcatctagct tttctatttt acgtagatca atagagatcc aatgttcttt
    85201 attcatgtga taggctggaa aatacccctc ttttcctcgc aaaattccgc caagttcagg
    85261 atcgaccttc aaatcaagaa tattgatgat cctatggtca cttgcatcat catagatttt
    85321 ttcttctgga acagacatca ggaccccaaa ccattttttg cttttttgat gtctgaatac
    85381 gcaataatca ggaaattttt taaaaatata ttctggagac aaattgtatg tttcattgat
    85441 gtgatcgatt agttcttttt tcgttagcat ctgcttccat ctctttctta ttgattcgct
    85501 ttattcaaag ataccacgta agaatagaaa aatgaataca agtagttgct aatcaaatac
    85561 ttcgatattt ttctttttta tcaactctta aatgttcaat ccgcctgtgt tggtcttgct
    85621 accatctact attaaaatgt ttattattgt ttgcccacct attttttgat tttagtggta
    85681 tcatttttca agttcatgat gaaattctta tcatcagtaa atgctccaga aaaataaaca
    85741 atgattgaaa aatcagttga aacttgacca ccgtttattt tctccgtatt cttagaatag
    85801 aagggagtct gattgatgta ttcactaatg aaaaaaatca ttaccgaaaa agatattcga
    85861 cgtcatgttt ctttagtaga acaattgctg aatcatacaa agataaccgt aaatgaatta
    85921 gctgaaatca tagggacaac cgaacgaacg attttttctg accttcaatc tattcgctct
    85981 cagctgcccg aaggctggga catcatcagc gatcaagcag gaatcagttt gcaaaaccag
    86041 caaaatttat taacaaatga cctttgggag atttttttca aacaatcagt cagtgtcgaa
    86101 ctgctgaaaa atctgctttt tacaaaaaaa gttgctgtcc ctgattttct agccgaccat
    86161 gggctttctt atgggacatt gaaaagacat gtgactaaaa tcaatcagcg tttagcaagt
    86221 tacgacttgc agatcgattt gacgaaatat acagcttgta ttcttggaaa agagcgtgcg
    86281 attcgtactt tttatcatcg attgctgatt cctttcactc ataacaacta ttttttcgag
    86341 gattattcga tccatgaatc ccattatttt caatttttaa gaaacctcag tcaaactgaa
    86401 ctagcggttg aaacagaaga gattttcgga acatgttggt tctttatcaa tacgatccgt
    86461 ataaaagcga attgccgatt agattcttct atccatacca acagcacact ctcctccctg
    86521 tatgactctg cattgaaaaa attgtattta acggaaggta tttatcttaa agacactgag
    86581 ctttcttttg cttctttctg ctttctggaa agttggaatt acaacaataa ctatggacaa
    86641 gaaaccgctc gctgcctgca tcattctcca tttttagagg tcctcgaaac gtttgtagag
    86701 gaactagctt cagagctgtc tcttgatcaa ctaaaaaaaa catctctgac tgataacttg
    86761 gctctcctca tattaaaata tcatgaatcg ccgatattga ttgaagcatt ggaccgtcaa
    86821 taccatcgtt ttttagaaac atatgatcag caatatgcac acttgtatca acagaaagag
    86881 aatttactga acgatctaaa aaaagaaata gaaatcaatg aaccaaatta ctttctccaa
    86941 ctcttgtctc tactgatcca acaaacgatt ttttctatca aaccgaattt gttcaatgtc
    87001 tattttattt tccaaggaga accttcatgg aaggcgttct tgcagcagga attaaaagat
    87061 tacttaggaa aacgtgtctg cttcttacct atcgaactaa ccgatctttc cactatttct
    87121 tttgaaaaaa cagatatctt gatttccaat ttccctctag attctctaga tatccctatt
    87181 gtatacattt cttctattcc aacgaaaaat gagctcaatc gtttgactga actcactttc
    87241 aaaacttttt tatagtaacg gctagcgact gtattctcta gctgtttttt cttttttgcg
    87301 aaacagtttg ttcatgatcc ctttttctat ttctacaact aagaatagaa gaatcacatt
    87361 tgctccaatc aataactgtt gtgtcatcgt taaacttact gtaccgatca gctgctggcc
    87421 gactggtaga aaaataacca tcccttgcaa taaagcaagg atacctaatg aaagaaaaag
    87481 cgctttgttt tgcaaaagac cttggttcaa tgacggcttg gacaactctc tgcaattgat
    87541 catgtaaaca gcttgagcta agacgatatt ttgcagaaga atggtttgct gtaagctttg
    87601 cccttcaaac tgcatagcca ataaataggc aggaaccatg atcagtaggg agacatagat
    87661 gatccgaaac aaatgataac cagataggat tccttgcttt ggatctcttg gtgggcgctt
    87721 catgatttct ggatctggcg actcgaaccc tagtgcataa gataaagtga ttgtcgttac
    87781 catattgacc cataaaatct gtactgggga caaaggtagc gggcgattca gcataagtgc
    87841 ccaaatcaag atcaatcctt gagctaatgc agttggcaag aagaaattga tggttttttg
    87901 caaattatcc aaaatccgcc ggccctcttt tacggctttt gcaatcgtat ggaaattatc
    87961 atctcctaaa accatatcag ctgcctgttt gcttacttca ctacctttga ttcccatagc
    88021 aattcctaca tcggatcttt tcaaagcagg tgcatcattt acgccgtctc cggtcattcc
    88081 gacgatttct ccatttttct gcaaagcttt aacaattcgc agtttatgtt ctggtgtcgt
    88141 tcgagcaaag acatcgacta ttggcacttg ctgaatcaac tcttcatcag acataagatc
    88201 caaatcgatc ccttctaaaa ctttggcagt atgttttaag ccaatttgtt ccgcaatcgc
    88261 ttttgctgtg tctttgtgat cccctgtaat cattttcact gagatccctg cttgttggca
    88321 ttcctttaca gctttgactg cactttcttt tggcggatcg atcaatccgg cgatccctgc
    88381 aaaagttaat tgggttaaag tctcatgcgt gagttcctgt ttcgaatcta cttttttata
    88441 agcaaatcct aaaacacgct gaccttttct cgcaaattct gcagctgtct gactccatgc
    88501 ttcttgttct gcagaagaaa gagttgattt agcaaataat acttctggtg cacctttgac
    88561 gtaaatcacc gctccttttt cctccgcatg catcgttgcc atatatttgt attcagaact
    88621 gaacggaatc tttgcttcta tttttttcaa aggtagattg gctgtttctg tgtgtcgaat
    88681 cagcgcaact tctgtcggat tgccacttaa attttctgtt ttctgttctt cttttgtttt
    88741 taattcctga cagtttttca taatggacaa cacgcaggct tcttcttttg cagctacatc
    88801 cacaactgtc atttcatttt ttgtcaatgt tcctgtctta tctgaacaga tcactgtcat
    88861 agaacctaat gtttctaccg aaggaagtcc tttgatgatc gccttttctt ttgccatttc
    88921 atgtactcca acggatagaa tcatggtcaa aacagctggc aatccttcgg gtaccattga
    88981 caccaccaaa gcaatcgtag ccgaaagaag gatatgccaa tccataccat atcgaaatgt
    89041 cgtaaaaata actaagaata ggctcaaaca aagaatgcct tgaaaaattt gtttgtttaa
    89101 tcggttgatt tttttaatca gaggcgtcgt ttgttgttcg accgattgca atgcttgatt
    89161 gattttaccg atttctgtcg tgtttcctgt ttctacaacg acaccgactg cagaaccaga
    89221 ttgtacaagc gttcctgaaa aagccatatt tttctggtcg ccgatcgatt cttcttctgt
    89281 caaaacggca tgactttttt cagatgcttc tgattctccc gtcaaaatcg attcttccac
    89341 taataaatca tgtgcttcga ttaatcggat atctgctgga acgacatctc cggcacgtaa
    89401 gacaatgaca tctcctttta ccagttcttt cgcagggatt ttttcttgca tcccgtttga
    89461 tagtactatc gcttcttgtc ccatcagctg cttcaaacca ttcaatgatt cttccgcttt
    89521 tctttcttgc cagtaactaa cgagtccatt gacgatcact acgaataaaa taatacttcc
    89581 ttcaatatat tcactagaag caaatttcaa aaaagaggca atgatcaaca cgatcatcag
    89641 cagatcagta aaatgctttg ctattttttt ccatcgtttt gtctgctgcc ttgcttttat
    89701 ttcattatat ccttgatcag tcaggcgttt ctgtcgttct tcttgcgaca agccttctgt
    89761 tgatgtttga tagtttttca atacatcttc taccgtcaat ctgtaccagt tcatcctatc
    89821 ccctcctgtt taattgatac tgttattata cgcagggaag ttttttcagg cgtatcgatt
    89881 atttcagggg ggtctgaaat aattcgtttt gcaaaatgta tctgggccta actgatattt
    89941 ctattgctta ttcttttata tacaaaattt aagaatacaa accagataac caataaaaaa
    90001 ggaataaagc gtgtcgtgct ttcgaataac aagctgaaag caattgaagt aaaaaacacc
    90061 aacaaagcaa gatctttata tctttgtgta gtgttccgtt tcttttcttt aataaattga
    90121 acatgtgcac acaagatcga agaccacaca aatagaaaac aactcgttgt catgcctgcc
    90181 aaaagctgaa aaaacaatcg actatcagct acaaaaaaat gtagacaaag accggcaaaa
    90241 aataacattc ctgtaattaa aataccgtgt aaaggtacgc ctttcttaga aagcgcacaa
    90301 aaccaatggg gcaaactttt ttcttttgct gtttgatata aaatcctgct agtactgtag
    90361 actgcactat ttgctgagga caaagaagca accaaaatca ttagattgac aattgctgct
    90421 ccgccttggc aatggatcac ttgcagcata gtcactaaag gactttcttt taaatctaaa
    90481 caatgccatg gaacaacaaa taaaatcgac aaaatcgttc ctatatagaa aaagagaata
    90541 cgccaaggca gacagttgat tgcttttttc aaattctttt gcggatcact tgtctcgcca
    90601 acggttaagc cgatcatttc aattccagca aaactgaata aaaccatagg aaatgagact
    90661 accattccag caaagccaaa cggtagtaat tcatcgatct gtccaaaatt ttctttgatt
    90721 ccgatagact gggtatgaaa atctgagaca ctcaatccta tacccaataa gataaaaagg
    90781 aaaatcccca agatcttgat gattgagaaa aaagattcca attcaccaaa ccacttgact
    90841 gcaacaaggt tgactgccat taagatcatt agtgcgctac ctccgcttat caaagctggt
    90901 acatgtacaa accagaaatg gatatacaat cctattgctg ttaattccgc aagtccagct
    90961 ccaagccagc aaagccaata agtccaattg acaaaaaatg cccacttttt gcctaaataa
    91021 tagtcaacga aatcactgat agagttaaat gtactatttg ctaaaaataa atcacccatg
    91081 gcccgcatca aaccataaat agccaaaccg catatcaaat aaacgagaat aacagctggc
    91141 cctgctgatg caattgcctt tgctgaacct aaaaataatc ctgttccaat tgcgccacct
    91201 aatgcgatta actggatatt tcgattagtt agagtccgtt tcattgccgg tttttccatc
    91261 aagttcacct ttttcttttt caagcagcat ggcgacttcc tgttcccaag aaggtttctt
    91321 tattttttca atacagagtg gtactacaaa caaaatgaca ctgatggctg tgactgcaat
    91381 tagattgatc catgttccag ctttcatcgt cagtccgaca gaaaatccga ttgccgcaaa
    91441 taaaacaata acgacagtcc aaactccaag attgctcttt ttctcttttt ttccaattct
    91501 aaatggtcga ttcaactttg catgtgtata acgcaatctc aaaactgcga tccccatgat
    91561 gacatagacg atacagtaga taacagtagt tgaatttacg agcatcaaaa atgcttgatt
    91621 gacgccagga atcagcaaat aaaaaagtgc aaagattgag ataatgacag actgagtcaa
    91681 aataacggat ttggatacgt caaattgatt cgtcttccaa aaattgaatt ttggtggata
    91741 cgcacctttt ctagcacttt ctgtcactgt ctttgctggt cctgaggccc aagcagataa
    91801 ttggactgct acaccaatga agacaagtaa actaaacaga ttcacaataa gatgtggcaa
    91861 accaagaatt tggcaataaa tcgaaataga ttgcgcgata ttattcaatt ccatttgtcc
    91921 ttttggtacg acatttgcta caactaaagc attcaaaata ttgacgaaaa atataaaaat
    91981 taacgcggca aatactccgc gaatatacgt tttaactgga ttttccaatc gagtgatata
    92041 cacagaggac atttcgatac cagtaaaaat aaacatgatc ggcgcaaaat aaacaaatga
    92101 ggatgcagtc gtagtatctg gtattaattt gtcccatgaa aaactgccta atgttccggt
    92161 tggtacgatc ccaactttga tccacgcagc aaaccctaat aagagcatca tcgtcaaagg
    92221 cacgtacaat cctaaccaaa caccgacttt tccgccgatt tttgccatat caaatttcaa
    92281 attcaaaaga gtgatgatcc aataaacgac taaaatgaca actaatgtaa atttgctgtt
    92341 caaaccaagc ggcacattat cgataatatt accaaacaac ggagcgagtg ctgaagcgac
    92401 catcaccatc ccaggaaaca tttggaccca taacagccat gagacaacga acccccattt
    92461 ttttcctaga gcattcgata cccaaagttc tggaccacct ttttggggaa acattccagc
    92521 aaattcacca gagatcaatg agattggaaa agcgtacaat aatactgcaa ccagcatgta
    92581 aaaaatcatc gtccagctcg ttgctgctac atctggaata tttcgtacac ttgctacaag
    92641 ggcgcaggtc attccgataa aggtcatggt cgtgatttgc tttgatgaac cagtatcttt
    92701 catctttatt ccttctttca atagcgacat atgtttttgt tttcttttat ttttgttcaa
    92761 gaacagaaat gacaaggttt catcgattca gtacaaaacc cagtatcacc gaaacatcca
    92821 ttgaacgatc ttagtgaact gggtttgata catgaaacga acgatccaac ttatagaacg
    92881 ttttataaaa aagaatatct tggcgaggaa tatatgaaaa acagggggaa atacaattgt
    92941 tttgatgaag caaataattg tctgttctcc atatgtctct tgttattctt tcacacaaaa
    93001 atcagttcta ttggatgatt tcgtttagtt tcttttccat tgctttggcg attttttttg
    93061 tataaaaatt gaaaattcga tcatcattca aataaggagt catgatagcc gcacgtagca
    93121 gagtgatttt ctgttctttt ttccattctt ctttcgttag acccattctt tcaacaaaac
    93181 gaatcggaga atcaccgtaa tcttcttgtg taaatgtagt atgagaagta ataaaccgtt
    93241 caccataaac atcgccatcc atataagaag accgatcgaa cattttttca tttaattcat
    93301 tgatagcgtt caaatctgta cagccttgtt ctttgaatac ccaattgacc atattaaaat
    93361 caggatgatt caatggatag acttcgattg tttttccttt aacagtaaaa gtcagatgat
    93421 cgagaaattc tctgaatctt tgagctgcct cgattgaagc cccaatcaat tgaccatatc
    93481 cggtcacatt caatggcaat acacgatgtg ctgtccagac tgcggcggcg gttgctccag
    93541 cttttgatcc ttccaaaata taagctccta acatatctgg agcctttact gatttttcaa
    93601 acacatatgg cgcgaaataa gaaatgatat ttctcatatt tttatgtttg attacgattc
    93661 ctccagcagc ataagggaca tagcccatct tatgaggatc tatcgtgaca gaatccgctt
    93721 ctgaaatagc tctgaaccct tcatatacct ctttgtcaat cgttacgcag tggtggaaaa
    93781 catgatgttc ttcaaagaat tcagctaagg aagcatacgg cacaaattct cctgcttcgt
    93841 ttagaaacag tgaacgcgca tagccgccat atgcggcatc aacatgtaaa tagaaataga
    93901 tcccttcatc ttttaacctt tctcttaatt ggacgatttt atctacactg tctacttgac
    93961 cttcttctgt cgttcctacg acagcaacca cacctagaat tgggattttt tgtccagcta
    94021 attcacgaat tgttttttcc aaagcattaa tatccattcg ataatcctct tgaactggga
    94081 tagcaaccat ctgatctaag ccgactccgc aaatatctaa cgccttcatc caagaatagt
    94141 gttttgtttg agggaccagc catttcccta atctttggat attttttccg ctacgggaag
    94201 aagctgcttt gacctcatcc atttcttcgt ccgtaaagct ttcggtcatt tccaaaattt
    94261 cttcaacaga taaattcaaa agttcccact cagacatttt tttcactttt tcaggcagca
    94321 cttccttgac agctaaagga atcgatttga tgcaacgggc gtaccataat ccttcaagat
    94381 tagcaataga accatctgct gcaatgtgtc cccatccgtc tgtcatattg aataatgaag
    94441 caaaatcctg tcctacttct gcttccattt gcgaagttgc catagaagat tccaatgcaa
    94501 cattatttgg gttccacagc atcgcatagt tgtaagctaa taaagcaggc atcaatgttt
    94561 cagcattcat ttgtccccag taacggcctg ctgaatgcca tgggatcgat cctgcgcgta
    94621 atcgctgact gacttcatcc aagacctcta tcatatggtc gcgggtggct agataatctg
    94681 gagtgagctt atctccttcg ctaattgcag gcatatctga aggaatataa ttttctctcc
    94741 atccaagatg ttcatctacc atcttgttca acaacatttt ataaactggt ccattttcag
    94801 ctttgtcgcc tataaagacg gccttgatat ccatatcttt catttttctt ccctccatat
    94861 tttgttggtc aatgtcttgc agatacatgt gtttatctgt aacacctctt cactatagcg
    94921 ccaaaaatga gaaatttttt tttataacat caaatccacg tagattttta caaacaaaaa
    94981 taaataaagt cggatttttc tgataattca acgcaaaaaa gcgaacaatt tttcaataaa
    95041 tcagtcttct aaaatagata acctaaaaaa agcgaacaaa caaataaaat gactgtcctt
    95101 cattcactaa acatattttt atttttttat attgttaaaa attcacaatt tattttttaa
    95161 tagaattatc tgttttccaa attttataac ataaaaaacc ttttttaacg gcatttatat
    95221 gctttttcag aaattaactg atgatttcaa aaagaaaaca tgtcataata tttcccatat
    95281 tgtgcaaaaa aatacaaggc ggaggctcta ctatgattga agaatatatt gagaaaaata
    95341 ttttaagaca gctgttttta tgtgggcaat tctatgtaaa taaagaagtc aatctagaaa
    95401 agctctccaa tctcctgcat gtttgcaaaa ccactttact caatgatatc aataacataa
    95461 aaaaagaatt tgaagaacag atcgcttata cacatcggga aaaggaccgt tacactttat
    95521 atttttcaga acatatacct cgatgcaaga tcatgcagca attgtctcaa aattcgttgt
    95581 ttttaaaaac ttgtctgctt tatttggaag aagatgaacc tgattatctt caattaactg
    95641 aatgtgaatt catctctgtc agcaaagctt attcattaaa aaaacaagta ttagcttact
    95701 ttaatgactg cgggatcgaa atcgaccgct actcccctcg attcactgaa atggaaagac
    95761 gtctgctgct tcttaatgtg tcgtatcgtt taggcggttt taattcttgg gagctacctg
    95821 aatcgttttt tgaacgtgcc gatcgtttca ttgaatccgt aacggaaaat agtggcagat
    95881 tttatgataa agaaaataaa gaaatcttat cgattggttt tgctatcagt ttcctacgcc
    95941 agcaagtgtg tgctgtgaca atagattcaa aatttatcga agaaatcaaa aaacgtccta
    96001 tttacaatta tgtcgaatca gcatgggaaa acactgattt ccaaacctat tataaaaaag
    96061 aagaattcgc ctttattttg acactattca atctttgtaa ttatggtttc cattcttatc
    96121 agttgattgc ggaagacttt caacagctac accaagtgtt tattgacaat actcccgaaa
    96181 taaaagaact agttgctacc tttgaatctc acttcaatca agaattattc ggaaaccaac
    96241 cgttcgaacg tgcgctgatt catctgatgc gttcggcttg ggataactat caattattca
    96301 tgccagaaaa attttatctt ttaaatgagg aacagacaaa tttatataaa gaagtccaga
    96361 caattttttc cagctggtcc agccagttac cttatgatct acggttgaat cctaattgca
    96421 tgcgggcttt tgtgatcgaa ttgagcggaa ttctccgtct gacaaaagaa catctgacta
    96481 tctatatcgt cacaaattcg gatgttcatt atctgattta tcgggaagcc ttggaagctg
    96541 taactacttt cgattttcaa gttgcaccaa caatttattc aagtatatct gatataaaga
    96601 agtatgcgca gcagtcctca aaccgggtat tatgtgaacg tacattatat acaccagatg
    96661 cggtccaata tgaaaatatt attcctattt cgatcaatac gatcgatcga gctatcattt
    96721 cagctgtgca aaacaaataa ggggctgtga caaaaatcat gtcacagcct cttatttgtt
    96781 gaagatgccc atttctgtaa caatctcttg atacacgtca ggctcacaac ctcacttatt
    96841 ttggacatct aggggtccga tctctttatt taaaaaataa gaaatgatgg ttcggataac
    96901 taccaacgcc gctagttgga aaatatcctg caaggtaggt ttgataatcg attcgatgat
    96961 cccagctgaa acaagaattt ccaaacttag aagtatatag cctcccaaca tctttttaat
    97021 agcattgttc tcgcttactc ggtcgctttg cacttttgta tggacaaata aatatttcaa
    97081 aaaatccttg attgatagac aaacacccca aaccaataaa cagatagcaa agatatcaag
    97141 tcccagtaca agtttttcga aatatggaag aagaaactgc atcaactctg tcgtcatcct
    97201 acctctcctc ttctttgttt ttctttgtct tctatttcct gattcaagaa ataagaaatg
    97261 actgttcgaa tgataataat aagtgcaagc ttcaaaatat ctgtccaagt cggcttgata
    97321 actgactcga tgatatcagc aacgactagt acttccaagc ttaacaaaat ataacttgct
    97381 agcagttttc ttgtatctgc attacgagta ttacgttctg catagttttt cttgacatga
    97441 gaccaatcaa aaagtttgaa tagaacaaga acgatcccga caattaagat accgatagaa
    97501 caaatattta aaatagcaat gatccattct agaaattcaa ttaaatttga gcgaaacata
    97561 tacgctacct actttctttt aatttgttcc tattctttta ttgtaaaaga aagttacctt
    97621 gttcgtctat ttaaaagttg ttctaatgat aacggttcgc ttaaattttt ctttttcact
    97681 ctcccatttt tctccctcca tccataaatc gtggaagtat ccaaaacaac gtagcaagtt
    97741 aaaataaggc ttagtccgtc atcagctttt caaagaagca ctgtctcggt gctttttttg
    97801 tacccaaatt tctaaaaata gaaccataca cctcctcaaa tcttttcttc ttacctaccc
    97861 tatttttatt tacaggaaaa tcagacactt cttctttttt gtcaaaattt cctttttctt
    97921 ctcgtcctat catttggcat tctaataaaa acgaactatc cttggatata gaaagagaaa
    97981 acaaggaggc gtttttcatg agtaaaaaat taatcaaagt cggcatcggt ttaggtttgc
    98041 ttgcccttgg cgctgcatat ctaggaaaaa agacaggact attcgaagat gacagtcatt
    98101 tatatgatga attcgaatca atctagcatc ctatttagtt gaatcactac ggacatactt
    98161 ggaagacatt ggaggaatca caatgagaaa aagtaaagca ataatgattg gtgccggtat
    98221 tgccaatatg gcagcggctg tttatttgat tcaagaaggc aaatggaaag gggatcagat
    98281 caccttttat tcattggatg atcacggttc aaatgatggt gcaccgaccg acactgtagt
    98341 ggatgaatat tggaataaaa accatccatt agaaaaccaa aaaggctata ttgctcgagg
    98401 cggtcgcatg cttaattacc gtacctatgt agatttgatg gatctgttaa gtcgaatacc
    98461 ttctgttact gagccaggga tgactgctga agaagatacg agagattttg atgcaaaaca
    98521 ccgaaccttt gacaaagcac gtttgatgga aggtggcaaa ggaatcattg atggcggaaa
    98581 actgggattc aataataaag accgtattct tctgactaaa ctgattgcta tgcctgattc
    98641 agaagaagaa aaactagaca atatcactat cgaagaatat ttcaaagatg atccacactt
    98701 ttttgaaaca aacttctggt ttatgtggga aacaacattt gctttcagaa cacgtagttc
    98761 tgctcaagaa ttgcgtcgct atatgcatca aatgatttat gaattcactc aaatcgaaca
    98821 tctagtgggc gtcaatcgaa cacgttacaa tcaatacgaa agtattatgc ttcctttgat
    98881 cgagtacttg aaagaacaag gatgcaaaat cattttgaac cgccgtgtca ctgactggga
    98941 attcaaagaa acaccgatgc aagatgaaat cactgtgact ggtttgaaaa ttaccaatac
    99001 actgactcaa gaagaagaat acgttgtggt cgatgaagat acagcagtta ttttcactaa
    99061 tgggtccatt acagattcag ctactttagg agattacgaa acaccagctg tagaaaatat
    99121 ggattatggt gcagcttcta gtctctggaa aaaagccagt gaacgtttct acaacttggg
    99181 aaatcctgac aaattctttg ctgatcgaga tgcaagcgaa tgggtcagct ttaccctgac
    99241 gacgaaaaac catttgttgt tgaatgaaat cacccgtatc acgactcaag aaccaggaaa
    99301 tgccttgaat tcatttattt caaccacacc gatcacgccg ttaggccaaa aagatgtcaa
    99361 tatgtctatt gtcgtgcatc atcaaccgca ttttacgtct cagaaaccaa atgaaacagt
    99421 tatttggggt tatttccttt atccaagacg acgtggcgaa tttgtggata aacagtatat
    99481 taaaatgaat ggaaaagaaa tgctagaaga actgattgga caactttcaa aagtagatcc
    99541 tggaccagtg aatattcgtg aaaaagaaac agaaattttc gacagcgtaa taaataatat
    99601 ccctgtctat atgccttatg cttccgcgtt gttcaataat cgtgccaaaa gcgatcgacc
    99661 aaaagtcatt cctaaacatt cgaccaatct ggcctttaca ggagaatttg tcgagcagcc
    99721 ttatcagatg atctttactg aacaaagtgc tgtacgttct ggagaaatcg ctgctttcca
    99781 ttttgcgggt attcctatgt cccgtcttgt caaaacaccg cgttacgaca aagatattcc
    99841 tactctagct cgtgccgcaa aaaaaatgtt tgaataatca agaggttgtg aaaaagctgc
    99901 cctgcatcaa gtaataagaa caaccaggct aaaacagctt cttatgtttt tgatttggtt
    99961 ggacttatta ccgcagatgc aagcttttga acaccgttta ccaagaggtt atgaaaaagc
   100021 tgccctgcat caagtaataa gagcagccaa gctaaaacag cttcttatgt ttttgacttg
   100081 gttagactta ttaccgcaga tgcaagcttt tgaacaccgt ttaccaagag gttatgaaaa
   100141 aggcgtttag tttcgagtat gaagaaaaat tttgtgaaaa tggttcctca cgtttttgca
   100201 taaatttgac ttaataccga gaaactgatc ccttctgtca taacctcttt ttatttatgt
   100261 ctgtttcttt ttgaaaacaa ctattttttc aacgtttact ctcttgccaa tccaacatca
   100321 tctaacggat acaactattt caatgattac cattacacta aaaaatcgga aacacacctc
   100381 tccgaacaca aatacataag actatagaat gtttcattta agaaaaaaag cgtttcatta
   100441 tcaaaaaggt aagatctcct ttaaaatgaa attatctgta gtactaataa tgcagacaat
   100501 tttatgaatc tgaaaggagc atctccaatg aaagcagtca cttggcaagg aaaacagaaa
   100561 atggaaatca gaaacgtaga tgatccaaaa atattagaac caacagatgc aatcatccgt
   100621 atcacagcaa cagccatttg cggctccgat ctgcatcttt accatcatgg tgaagcagtt
   100681 ttggaaaaag ggtatgttgt aggacacgaa ccaatgggaa tcgttgaaga agtgggatct
   100741 caagttcaaa cattgaaaaa agggcaacgt gtggttatcc cttttaatat tagttgtggt
   100801 cattgtcatt tctgcctgaa tcacatggaa agccaatgtg atgaatcaaa tccagatcag
   100861 ttatatggcg ggcttttcgg atttggcaag ttgaacggta atcattgggg tggtcaagcg
   100921 gaatatctaa gagttcctta tgcagactct acttcatttg ttgtaccaga taatgatctt
   100981 cctgatgaaa aagttttatt tctatcagat attttgccca cagcttattg gagcgttgaa
   101041 aacgcaggag taaaaaaagg cgatacagtt gtgattctag gttctggtcc agtcggtcta
   101101 ttcgctcaaa aattcgctgt tatggctgga gcagagcgcg taatcgcagt agatccagta
   101161 cagcaccgtc tagataaagc ggctaattac aatggggttg aaacttattt cttagaagac
   101221 acggcacaag ctggaacaga attgtatgaa ctggctaaag gtggcgcaga tgtcattatc
   101281 gattgtgtag gaatggacgg attagagcct gtcaaagaaa aagcgaaaaa tttagtaagc
   101341 ttacaaagcg gcacaatctc tccattacag atggcatcgc aagctgtcaa aaaatttgga
   101401 acagtccaag taactggtgt ttatatgact ccagcttctt cttacccgct gcaagaattt
   101461 tttatgcggg atatcgctgt caagcatgga caggcaccaa ttatccatct aatgccaaaa
   101521 atttacgcaa tgattgcaga aggactattc gatcctacac aaatcattac tcattcgctg
   101581 ccactatcag aagcagcaca agcatatgag atctttgaca aaaaagaaga taaaaatata
   101641 aaagttgttt tgaaaccttg aattcagtaa aaaatacagg tgattttctt tatgcccaaa
   101701 atcaaacgaa atacccatga gatcgaaata ggaaaagaag aagatatcga aatacgagga
   101761 cgtcgcttcc gcttctattt ccaaaatcgt tacacattga tctcactggc tgtcgattta
   101821 ttaaccggga ttttctatat ttttggaagc cttgcttcct taactaaaat ccccgatcga
   101881 tatggcacct atttttattt ggctggtgct attttcttga cgattcgtcc tattctcaga
   101941 atcgtaagga acatttttat ttacgacgaa aaaaaagcaa tgaagagaac aagcaaaaaa
   102001 aaagaggcat gagaattttt caaatctcat gcctcttttt tcaccctatc gtatcacttt
   102061 gatacagacg agcggtcgtc ccactttctt ccttgaccat tgtcaaaaat gtccactcgt
   102121 atcgttcata aaacgtggta tgatctgtaa ttaagtaaag tttttcgtac ccaagttgat
   102181 gcatttcttt tctgatttcc gctaatatcc gcttggctat cccctgctta cgcttttccg
   102241 gttcaacaaa cagggcacaa agatttggtg tcagatcagg gcggttatgg aagtcgtttt
   102301 caatgacgcc cgctcctccg acgatggttt ctttttcatc taagaccaca taccattgag
   102361 gaatactttc cttattagcg attccagtac gaatactttc ctcatacaca acagcaggaa
   102421 ctccccattt tgatgaaaac caattcgcgc tttgttttac taattctggg tgttctctta
   102481 atttgatcca gttatatgta accaatgttt aaacctccga ctaaaataaa acgtttacaa
   102541 aaattataca agtctttatg aaaattagca atgacaacaa tagtgatgta aaaaaaaaaa
   102601 cggaaaacaa tcacttgttc tccgtttttt gattaatcaa gcatctaaag cttcttttct
   102661 tctttcttgg atcgtagcta ccttgacgaa cggtagatag atcaaaatag aaagaaccaa
   102721 attgacagcg gctagtacac cacctgcaat actttttgtt gccaatacac cgccaataat
   102781 tggtggagtt acccatggag gcataacggt agctgctggc actaaacctg tagaagtcgc
   102841 aaagtaagca acactaacta agaccattgg tgtcaaaata aatggaatga acatgatcgg
   102901 gttcaacacg ataggtaacc caaacatcgt tggttcatta atattgaaga cacctggcgc
   102961 aatcgacaag tttgttacaa ccatatatgg tttgttttta cgtcctacta aataaatagc
   103021 taacagtaaa cctaaagttg cacctgttcc tcctaagttg acaaagctgt caaagaaagg
   103081 tttgttcacg ataaacggtg cgctatgtcc agctgcgatt gcattaacgt ttgcttcaat
   103141 ggctggcgca ttgattgttt gcatcaaagg atcgaccata ttcgctccgt gtaaaccaaa
   103201 gaaccataag aatggtgtga taaatgcaag cagtaaggca gatggataag agtttgcaag
   103261 tcccataaat ggttcttgga ccactgtata gaaagaacct acgatatcat caacaccgaa
   103321 tgcagcaaaa attgcagcaa ctaaagcaaa cgcagagata gtgatcattg ctggtagtaa
   103381 tgaagcaaat gcttttgcaa ctgccggtgg cacaccatca ggcattttga tgatcaattt
   103441 ttcatttccg cttaaacgta caaagatttc tgttgaaata atcccaacaa taatagcaat
   103501 aaacaaaccg tttgagctca tacctaatgc tccgcctaaa gcgaagaaag agcctaaaga
   103561 aatcacacct gcggaaacac cgtcttttcg gtagccgtta gctaaatgat aagcaagcaa
   103621 gaaagcaatc aagacagaaa gaatggcaaa tgtaccgttc caagcagcag ccccgaatcc
   103681 tttccatgat tcaccaccaa aaattgagtt catcaattct tggaacccgg gaatcggtaa
   103741 gttattgatc attactgcta atgctcctaa gatcattaac ggcatactca ccataaatga
   103801 gtcccgaatc gcaactaaat gccgttgtgc accaattttt gatgctacag gtataaaatg
   103861 tttctccata aatgcaacaa atttatccat aaatcttccc tcttttcttt actgtagatt
   103921 acgcgttcaa tcgacgatac agattaatga tctcttttgc taaatctgta aaagctatag
   103981 atgtcatcag atgatcttga ctgtggaccg tcaaaagcgt gacttcaacg tgatttcctt
   104041 gtgcttcttg tgtcagcatc tctgtttgag agtgatgtgc ttggactaaa gattcctctg
   104101 catctttgat tttttcatct gctaaagtga aatcaccttt tttcgcagca gcaatcgctt
   104161 ccattgcatc gctttttgca tttccgccaa acatgatcag acccatgatg gcttctagat
   104221 ttttttgatc ttccatttat tgtatcctcc gataattttt attatttgtt tattagtttt
   104281 tctgcttggt cgagtacttt ttcaccattc atcatgccat agtcagccat attgattaca
   104341 tctaaaggaa tgcctttagg tgctagcttt tcagcaaatt gattttgcat aaagcgtact
   104401 tgcggtccta aaagtaaaac gtctacattt ttagattcta gttgattatc cgcatctgaa
   104461 gcagatacag caaagatgtc tgcttccatt ccttttgctt ctgctgcctt ttgcattttt
   104521 gtgactagga gacttgtact cattccagct gaacatacaa gcatgattgt tttcttttcc
   104581 attttaaaca cgttcctttc tatacttaca cttgtatata agcaagtagc gtgccaactt
   104641 taaaagcgct ttaaaaagtt gatatcatca catttaaggt ctataccaag ttgcatacac
   104701 actaaaaaag aaagcgcaca ctaaacacac aaattaattt ttattacctt ttttctttta
   104761 acgtgcacaa aaaaagttgt gacacaaaac ttgtcacaac ttttttcaaa taaacggtat
   104821 tcaaaagctt gcatctgcgg taataagtcc aaccaagtta aaaacataag atgttgtttt
   104881 tactttgcta ttcttattac ttgatgcagg acagcttttt cacaacctct caataaacgg
   104941 tgttcaatca gctgaccctg ggtattagct caaaacgctg gattcacaac ctcttttatt
   105001 tcaaataacc agtaaataca atgaacatct acaaaaaact tctatttatt tatagatttc
   105061 gtcaggcaaa tccaattctc tcttatcaat caaagtctga taccacgaag cagatttttt
   105121 caatgttctg cttcgttcat cgttcaattc gattcgaact aatccatatc gattcttaaa
   105181 tgcattcatt ggtgacacac agtcagtgaa tgcccataac atatagcctt cgcaatttgc
   105241 gccttcttct gttgctttga tcaagtaatg caaatgctgt ccgataaatt cgatacggta
   105301 atcatcttgc acgattcctt tctcatccat atactgttct tcatattctc ttcccatacc
   105361 gttttctgtt atcagccaag ggatattatg ataattttct tttagataca tggctatatc
   105421 atacatgatt ttcggataga tttcccatcc cctggaatta ttcattttct tcccaggtaa
   105481 atcaaagact tcataatatc gttcaggatg gaaaggtgtc tcttcattcc attgataacg
   105541 aggggcgcga acgcgtttag gataatattg attgattcca aggaaatcta ctgtatgatg
   105601 ctgaatcgtt tctaaatcct ctgttgcagg atcgaaataa atcgattgtt tttgacacaa
   105661 ctccaggaac tcttctgtgt agtatccttt taccatcgga tcaaaaaata ctcgattata
   105721 aaacaaatca tacatataag cagctttttg gtcttctgga gaattggatc gagcatagac
   105781 catttcagga tttaaaatac agccaatcct tccaccataa tttccgctgc ggtaaatctc
   105841 tactgcttta gccgttgcta aaactttatg ataattccac atcatccact tttttgtgtt
   105901 ttgttcatgc ggccagcgaa cagcatccaa atagcaacgc gtttggggaa cgattggttc
   105961 gttgaatgta aaccagtttt ttaccctatc accgtaacga tcaaaggcaa tttgagcata
   106021 cttagcataa aaatcaacta cttttcttga agaccagcca tcatattttt ccaaaagaaa
   106081 agctgggact tcataatgtt ccaaacaaat catcggttct acgtctgctt cgatcaaaga
   106141 gtcgatcatt tcacttatat gctgagcata ttcttcatct actatacagt tctcataatc
   106201 agtaaaaaaa cgagcccaat tgatagaagt ccgataatgt gtcagatgga tggatttcat
   106261 cagatccaca tcctgctggt attgttccat aaaattagtt gctacggctg ggccataccc
   106321 attatgccaa acaaaatgtt catttttata ccagctatct ataaacgaat cctgtccttc
   106381 tttttttcct ttccaacctt ccgtttgcca agcagaagat gcagcaccaa gaataaaatt
   106441 ttttggaata gtaattttca tgatttcctc ctgtttttta gatcccatca ttttccacac
   106501 gatttgctac ttttacaaat ggcagataga tcaaaataga aacaataata caaacaagtt
   106561 gcgtaataac tgcaccgata tttcctgctg ttgccagata agcattgacg atcggcggca
   106621 ttggccaagg gaccattaca actgctttcg ccgcaaatcc taaagcggtt gcaaaataac
   106681 caatcgttcc agtgataaga ggcgttaaga taaacggaat agctaaaatt ggattcagca
   106741 tgatcggcat accaaaaata actggttcat tgatgttgaa gatcccagga gcaagggata
   106801 acttggtgat ttgtttcata tcttcccgtt ttccaactaa caatactgca atcagtaaac
   106861 cgatcgtgac tccagacccg cccatactca tatacatatc ccaaaatggc atagtaataa
   106921 tgttcggtac ttcttttcca gcttcaaaag cttccgtatt tacagcaatt gccgctaaaa
   106981 gcaacggttc ccgtatcggc ttcaccattt gatttccatg gataccaatc acccaaaaga
   107041 cttgcgcaat aaacatcaat agcaaaatac ccggtaaccc ttgtacgaca ccttctaatg
   107101 ggcgttgtac gacattgtaa atgatatcat aaaggtacat tcctgtaagt tctttgatga
   107161 taaatcccgc agccgcgata cccgatacag tcagaatcgt cgggaataaa gcagaaaaac
   107221 tagcagaaac attcgttggc acggtatcag gcattctcag ctgtagtttc ttctgtttcc
   107281 tcaaccagca ataaagttca atagaaagga tggagaccaa cattcccaaa aataaccctt
   107341 tcgtatctgt atattgttta gccaatacgt tttctactat aacagtctga ttttcattca
   107401 ctaattcata aacagttgga ttaacagata aataagacat cactgcgatg ataccaggga
   107461 atacaccatc cactttattc agcttggcta attcgatccc tatcagaaaa acagcataga
   107521 tggtcaagaa acttagtgtg acatagctga tggcagatgc gataggcttt aattcttcta
   107581 aaaaacgcaa gaaacttatc tgtgctaagc cattttccga gtcaaagacc attgctgaaa
   107641 acaatgtgcc aaaagcaccc gtaataatta ccggaatcaa tgcagcaaat gcgtttttta
   107701 tcaccatgat ataccttaaa ttattgattc tcgttgccac gttccctaaa acattggcta
   107761 atttatccat aaaactcatt ccagatcccc tcattcctgc ttcttctcct tgacgtcatc
   107821 tctttttgtc tattagtcag cgcttacatt tcgatgaaca gaaactattt actcctcttt
   107881 tttaccaaat ataaaacaaa aattcatctg atgtatttat cataaaatac atcagatgaa
   107941 ctgtcaatta gtttcttttt ctcctattat tttctgtaat tctctccgat ttattgccat
   108001 atggacagtc attgcatcaa acgcagcaag tgcattccgc ctttcgatgg cctgaacgat
   108061 ctctgtatga atatcgatcg tctcgatttt cagatgctgg atatcataat ttttattaaa
   108121 caaagcaatt gattcattaa tgattgggat aatatgcagc aaggctacgt ttccgctagc
   108181 ttctgcaatc atcgtatgaa aatcgatgtc taattgaaca tgttcttgtg tgccttctcg
   108241 aaaagaagtt tcaatctttt gcgttaattt tttcatttgg acgatttgat tcttcgttgc
   108301 atattccgct gccaaacctg ccatcttagg ctccaataaa tatctcagtt caaataagtc
   108361 tcgtatcatc cgatcctgat cttcaatcaa agaaaaaccg agtgggtcat tggcaatccc
   108421 tcgcttatca ctgacaaatg tccccgctcc ttgccgaact tcaaggatat tcctggaagc
   108481 tagagcccgc acagcttctc gcaccgtgct tcgccccacc tctaaataat cggacaattc
   108541 atattcattc ggcagtttat ctcctacttg caactgcatt tcctttaaat atctaaggat
   108601 cttgtcagta gttacttcta ctagggtttt attttcttcc aaaccacata cctccttttc
   108661 atctgatgta ttttttcgta attattataa cacgtttgaa tcatctgatg attttttgta
   108721 tacaaaattg attaatccgt tcatagacat acaaacgaag aagatattta ccataaaaaa
   108781 gactaggata aaattcactc ctagccttcg atcattttta ttttgaacga caaaaaatat
   108841 gatgttcaat aacagacgat cttcattcga ttcttgatgt catcttttgt tttttttaat
   108901 atatggataa aaagtttgat tacatcgttt ctattctaga atttggtcga ttctcaattc
   108961 ttcttctggt gagaaatcta aattatccat cattcgaaca ctttcttgta actgacttaa
   109021 ccgacttgct ccgaccagta cacttgtaac agatttttgt cgcaaattcc atgcaagggc
   109081 catttgcgct aatgattgtc ctcgtgatac ggcgattgtt tgcagtgctt tgattttttc
   109141 cagagttgat ccaacttgtt cttctgaaag gaatggtatt tccttacgat gtgctctaga
   109201 atcttcagga ataccatgta gataacgatt tgtcagcaac ccttgagcaa gcggactgaa
   109261 cgtgattgcc cctagccctt cttcttctaa tactggagat agatcgtctt ctagtagtgc
   109321 acgagaaaac atattgtagc gcatctgatg aataatgaac ggtgcttcct ttctttttaa
   109381 aatttcagtc attttttttg tatcttctcc attatagttg gatataccaa tatataatgc
   109441 ttttccttgt cttacaagtt gcatcaaagc ttctgctgtt tcttccaaag gtgtatcagg
   109501 gtctgggcga tgatgataga agatgtcaac ataatctagc cccattcttt ggagactttg
   109561 gtcacaactt gaaatgatgg atttacgaga gccccattcc ccatatggcc cttcccacat
   109621 atgataacct gctttgctag aaataatcag ttcatcacgg taggcttgaa aatcttctct
   109681 aaaaatacgc ccaaagtttt tctctgcagc tccagcaggg cctccataat tattcgcaag
   109741 gtcgaaatgg gtaattccca tgtcgaatgc gcctcttaaa atctctcttt gattatggat
   109801 aggatcataa tcaccaaaat tttgccataa tccgagagat aaaatcggta atttcaaacc
   109861 tgaatgaccg gatttacgat attccatttt ttcataacgt gcactatctg caatatacaa
   109921 tttttctcct cctcatcaaa tctttttaaa acgctttctt ttttctattt ttagtttact
   109981 acattttaaa gaaaaggagt taatcaaaat gattttttcg tatatttttt tgccatttgt
   110041 tttttactcg ttgatagatc agaaattgat tacgtgcttg ctggattttt ttataacgtt
   110101 tcggttgttt tttgtaaaat tgctgatgat agttttctgc tggccaaaac gtaccggctg
   110161 gttcgatttt tgtgacgatt ggttggtgga aagttcctga actgtctagt ttctgttttg
   110221 attgttccgc taattctttt tgtcgttcga cttgataaaa aatcactgga cgatattgct
   110281 tccctctatc ttgaaattgt ccaaatgtat ctgtgggatc agtaatttgc caatagattg
   110341 tcagcaactc gctataactt atttctcttg tgtcataaat gatctccaca gcttccacat
   110401 gacctgtgta accaccactt acttgatcat aatttggttt ttcaacatgt ccgcccgtgt
   110461 atcctgacaa aacagatagg atcccttttt tcgattcaaa cggctcgacc atacaccaga
   110521 aacatccccc tgcaaatact gcacgttctt gatagtcagc atcttgcatg gcatgcttgg
   110581 aaaagtcata tttttgctcc ttatcaaatc cttccgtaat caataaataa aagtccatta
   110641 catcaggcgt cagattcatt cggatagcta atggacgtag tgttgcttct agttttgata
   110701 actgttcttt aacggatata ttttctctta tgtcattctt tgtctgaatc aatacttttc
   110761 gttcccaatc tcgtgtattc ggattcaaaa tcagattata aagtgtttga atgaatattt
   110821 gttcctcatc catagattct cctcctttcc gaagaagaca agctagtgac tttagctgta
   110881 atttatcata aagtgaggaa ataagcgatt acttcatccc cctacagatt tttcgtataa
   110941 aaaagagaga atccttatcg acagattctc ctttttaatc aatttatttt ataaatgaaa
   111001 agttaattta ccttttatgg aaaactattt taaaaaataa acataaatat gcagttaatg
   111061 taacaagata agaaataata gtcgttactg tataacttgt gcttacttta atatcagcaa
   111121 ctcctttgac tccgcttatt tgtaccaatc cattagcatt cacctgctcc tttacaggtt
   111181 gatgatttat ttctacattc gttcctaaat aagcatatac aggaagtgtg attacttggc
   111241 tcttctcatt agttactcta tatatgatag aactaccagt tgttttataa ggaaggttta
   111301 cccattgact gccaatcttt gcttcgtgtt ttattatatg ctccttattc tccaatgaag
   111361 cgataggaga ataatcatat aaaaaataat ccattccttt agtggtatac gcctctactg
   111421 tttctttgtt tacaacaata tgtcccggct gagaaattat tttttgattg aaattttttt
   111481 tactagaaac agttaaagtg attaataaca aaacagtcat aaataaataa aatagttctc
   111541 ttgtcttatt gtatcttcta tttttgaaaa atatcatcgt taatatcaga gaagagaata
   111601 ttatttgcaa gcttaagatc cgccaaggaa attgtaacaa tttggctggc gtattttgca
   111661 gaagatacca tggcaccaaa ctagttgtag ctagccaagt caaacacgta ccataataaa
   111721 tctttttttc tatgtttgtt aattttttaa ataaaatcag gggaatacat aaagataaca
   111781 gaccaactag tcccattgag taagtagtta agtcgttgtt catagcttca gtcagactat
   111841 cgaacacatt agatgcatga gcatataaat ttgtcacgct aggtttgttg attttttggt
   111901 agagcatttg ttcaaacatc ggataccaaa aagcactcgt cagaaccaat gtcattcctg
   111961 ccgcactaaa taatttttta aatatagcaa taaaatctgc attcttgaaa cctttagaaa
   112021 ccaaacgaat aaaaataaaa atgataatta atgctgtact cattaatgtt gatagtacat
   112081 ggctgtacac tagcaaactc atactcaatg ctagctttac agacggatac ttttcttctt
   112141 ttccagagat gatttgataa gcataaaata atacagggat aacaagtgtt attgcgatag
   112201 cttcggcaat tgcggaacga tagtagatgt ctactgtccg ataagcagag aaaatataga
   112261 gacaactaaa taaaaatgcc gctttttttt gttttaaaaa ccgctcgcca taatgataag
   112321 ctacgagtgt tgtacacaag ttcaataacc aaacgtaaat aatatagctt acaatcaaat
   112381 tttttgtcat ccaatataac attacagctg gaaaaaaagt cagaaaggga taaaaataat
   112441 ttacaccata tccagtataa ttgaatgtct cataattaat cggactggta aaaatagtcg
   112501 ataacccctt gattcgagat aagtgaaaag aaagatctcc agtggtataa atactctgtg
   112561 tatttttgaa aaagatgaaa gaatatatac tactaactat aattaatcca ctcaatatta
   112621 ctactttttt atttattttc attcttcttg tttatctcct ttttagtcac atatcgagaa
   112681 tctttcaact tattaggtgc aaatcgttta aaacttgaat acaccactaa acaataagtc
   112741 agaatactga tccataaagc catgagagca gttggagtat attttgttgt aatttctatc
   112801 ttattttctc cagcatttac atccacattc actaagccat gttttttata agagtcattt
   112861 acgactttac cgtttacttt tacctgtgta cctaaataag catatactgg tactgtcact
   112921 ttttgctttt tgttgcttgc cactgtgtaa gtaatgttat tatctgcagc tttatacttc
   112981 cctttttccc acgattctcc tacttttact tcatgttttt ctaagtgagg cctttctttt
   113041 atcgcttctg ttggggcgta atcgtataat cctcccatat ctgatgttgt ataacgtggg
   113101 agtgtttctt cattgataac aatatgaccg tgttttgatt ggataacttt tgcgtaattt
   113161 tcttttgctg ctatagacgt gacgagtaga aacaccacag tagcgccaat gattaaccat
   113221 gaatattttt tattcatagt ctttttcata aaaacaagcg ttatgatcat tgaagaaaaa
   113281 aagatttgga tacctaagat acgccaagga aactggagta agcttatcgg tgttttttga
   113341 aacaaaaacc atggaaataa attagttacg catacccatg tagaaactac gccaacatag
   113401 atgatttttt cttttttctc catttctttc agtatgaata aaggaaaaat caacgcaacc
   113461 aatcctaata accccatagt gtatgttgta atatcgttat ttaacgcttg aatgatacta
   113521 tctgaaatag aaagtgcttc tagctgcaaa tctgttttga agggactgtg tatttcttga
   113581 taattcatct gctgcaacat aggataccaa aaatagctag ttaatgcaca agtcaaaaaa
   113641 acagccttta ctaaattcac gaccacaaat tttatatcgt tttttatttg cttgcttaaa
   113701 aacaaacgaa ctataatcat acaagcaatc aatgcactac acaatagtgt agttaagaca
   113761 tgagtataca caagtagact tatactgaca gataaagcga tgactttttt ttctctattg
   113821 aatatcaatc cataagcata atataaaaca gctgggataa tcgtaagagc gattgcttct
   113881 gcaattgcgg atcgataata gatatcaact gttcgataag ttgcaaaagt atataaacaa
   113941 ctaaataaaa aagcagcatc tatcttttta aaaaactttt ctccataata aaaactaatc
   114001 agaatcgtac aaatatttaa taaccaaata tatagaacat aacttaatat taagttattt
   114061 gaaattccat aaaaaacgac tgcaggaatt attgtaagaa aaggataaaa atagttcagt
   114121 ccatctccat aattgttaaa tgttgtatag ttgattggcc cttcaaaaat agaagataac
   114181 cctttgattc gtgaaagatg aaaagataaa tcacctgttg tataaatttc ttgagcattt
   114241 ctcataaaat ttactgaata taaagtacta agtaagataa gaactactaa aagaaatgct
   114301 tttttactta ttttcataga tttgttgttc tcctttttta attctatttt cagtatacta
   114361 gaattgtctt tttttaataa gttttgcgtt aaatttttca tagcactagt atgatatact
   114421 agaaaagaat caatttttat tacttttaaa taggagaaaa aaatgaaaaa aaacaaaaac
   114481 caaataatag atatcatttt tatgttattt ctaagttttg cttacgtcat ccctctattc
   114541 atgtctaagg gaatctatca tagtgtcaat caggatactt actttcattt atctcgaatc
   114601 ataggactag ataatgtatg gagttctcca gtaaacttca ataacttcga tcatcatgga
   114661 acaatgatga atatttttta tccatggctt actctttatc cagctttttt attttataaa
   114721 atgatgggaa atcttgtttt aagttataat atctactatt tttttatcac ttttttaaca
   114781 atggtcgttt cttatttttc tatgaaacaa ataaaaaaca atcgttatat ttcgctgtta
   114841 ttttcgatta tttatacatt ttccgcctat cgtgccattg acatattcca tcgtgcttca
   114901 ttgggagaag ctgtcgcttt aacatttttg cctttgatac taatggggtg ttacgaaatt
   114961 tatattagag attaccaaaa atggtactgg ttgtctattg gcatgacgct agtagtgtat
   115021 actcatttac tttctgtcgc tatggtttct gtatttattg gaggaacgtt gtttctttct
   115081 ttttattttt gggaccaaaa aatcgctaga ctcttaagtc ttttaaaagc taccgtcctc
   115141 accttttttt taagtgcggg attcttaatt ccttttattc aacaatctag agcacaagaa
   115201 ttgaaagtcc cactgggaaa agaattaagt ggtatggctc ctagtgatat gctgacacat
   115261 attttaaata ataactataa caactatact attggattat tcttatttct cggattgatt
   115321 ggtgctttta tttttataaa aaaattaact gctgatgatt tatttatttt ctttcttggt
   115381 gtatttgttc ttttttgttc aaccaattta tttccttggc aactttttaa ccatacacca
   115441 gttaagagtc tacaatttgt ctggcggtta aatggttttt cttctttatt tatagcctat
   115501 acgatgagta tagtgatcta ttatattttt tctactaaaa ataattcatg gaaaataatg
   115561 gtctttacgt ttcttgcact aatattgcat ctctcaggag taagcaattc tatccatgct
   115621 aacaaagatt cattaacact tatagcacct gaagacgctg ttgctatagc tgaaaattac
   115681 aatcatactg attatgcaaa caaagagtca atctatcatc ctgatatgat taataatgat
   115741 caatatttac taggtaacca ggaaataaat ccaacaactc attttatgag taacaaactt
   115801 acaattgaat tagataattc gaataataaa aatactgtct tgacgacacc tatttatcgt
   115861 tataaagggc aagtggcttc tattaatggc aaactagtac aaacaaaatt atctaagttt
   115921 ggcacaactg aattaactat tcctccaggt ataaataaag tcgttattac ttatcagtac
   115981 acaaaactag caattgcttc aagatattta tctatcgtta ctttgatttt atttttactt
   116041 tatcgattta ctttttctaa acaaaaacaa ccgagaagag aagtacaaca ttctcactag
   116101 atttttattt aagaaagact ttttagctgt acacataaac aggctaaaaa gtcttttttt
   116161 atatttcttt aatttgcttc atacattatt taacatcgat atttatactg tgaaacatgc
   116221 gtttcatcca ttatcaaagt atttttatcg ataataaact tcatccaaaa acagaccttg
   116281 tgcagggacg gtttctcctg catgttgccg cactttgctt tcaaatattt catcgattgt
   116341 ttctatcggc atggcacctg tgccgatttc tagtaatgtc cccatgataa tccgcaccat
   116401 tttatataag aatccgtctc cgacaaaagt aaaatgaagc atttctccct ttcgttcgat
   116461 agataattca tcaatcgtac gcactgtgga tttttttgat tttttcaatg cagaaaaacc
   116521 aatgaaatca tgtgtaccca ctaatttttc gcaagctgca ttcagcttgt ccatatccaa
   116581 ctcttgaggg aaataaaagc tgtggtttcg ctcaaatgct gaaggaatca cgttgttcca
   116641 aacataatag ctgtattttt tgcccaccgc attatatctt gaatggaagc gctcaggcat
   116701 ttcttcgatt cttttgatga caatatcccg cggaaggtaa cgattcataa agtcgatcat
   116761 ttctgccagt ccaatttctg tgtttgtttt aaaatttgct acttgtcctc gcgcatgggt
   116821 gccagcatct gttcttcctg atccaataat ttctatctct tcatttgtca tctgatgcaa
   116881 tactttttcg atttttcctt gaatggtttt atcgtcatct ccaagtcttt gccatcctga
   116941 atatcttttt ccgtcatatt cgattgtcaa cttgatgttt ctcatatttt agtggctcct
   117001 ctgtcttcca tttggtcttc tctctacctt tttaagattg tagcacaaaa tatagctcct
   117061 tgcttctcta agacagaagc tatacaagaa aactgtggaa atatatattt cagaaaaaca
   117121 aaaaacagcc accggatgaa gtatccgctg actgttcaat ttgacggctc aagttttact
   117181 gtttgagatg caagatactc aacctggcaa tgggcacata attaaagaaa aaactgattc
   117241 tttggcaaaa acctatttta ttcagtaatg gccaagaatt tatagaagga attcttgcca
   117301 gtccataaat cgccgcaaaa agcaaactga tcacagacag tccaaggtat aagtggctta
   117361 cgtccatttg cccatttttt cgatagatca agaaaagaag aactggaaac aataaaaaac
   117421 cactgtaacc taaacctgat ccgatattat ggacccatgt cgagacattc cacgaagatt
   117481 catgggtatt gatactgaac aaacctgtaa agatacaatc acctatacca tagaacgcga
   117541 tggctgctgt aactagggta gctaacacac gagatgtact aacaaatgca gcatacaatg
   117601 caggcaatga caggatgaaa aagatccctg aaaccacaga ccaaataaga aacgcttttc
   117661 ttacaggact ggaaacctct ccaaatacac tgatcacgct cttcatttga tctaattcag
   117721 gataatacaa tcctaatacg tacggagtca aaaaatcact cagcacgcct aataataaaa
   117781 agaaaaaacc aaatttacgt aaaaaatcca tgatacgcct ccaataacaa ttacctatgc
   117841 ttttaattgt tattataaac gactttatct tgatttaaca gaaacctcta tttacacgaa
   117901 catctatata actcattaaa aactcgaatc attttaaaaa tactaaatat ctaacgttca
   117961 acgttcactt aatccatcta aacaatcttt caatttgatt ttttccccac ttcttttacg
   118021 cgcgcctagc tctcctattg cagtaaacaa cagatcactt gacgaattta aggcagtttc
   118081 cattgaatcc tggataacac cgataataaa tccaataccg acgatttgca tcgcgacatc
   118141 attagagata ccaaacaaac tacatgctaa tggaatcaat aacagtgaac cgcccgcaac
   118201 acctgaagct ccacaagcag ctaaagcaga caaaatacat aaaaggactt tcaaaacaaa
   118261 aggtgcttca atccctaaag aatgtacagc agtcaacgtc atcaaggaaa tggtaatcgc
   118321 ggcaccgccc atattgatcg tagcacctaa cgggatcgat atggaataag actcttttgt
   118381 caatttcaat ctttcagcca acatcatgtt tatcgggata tttgcggcag aacttcgggt
   118441 aaagaaagcg ggaagtgcgc tttctttcaa acaaaagaaa atcaatggat agggattttc
   118501 tttaataaac aaaaacgtga ggaatgggta cacaactaat gcgacaaaca gcatcgtacc
   118561 gatcaaaaca agtaaaagct gcagatactc tgccaatcca gcgattccag tcgtagcaat
   118621 tgattgataa accaatccta aaatccctat cggagcaaat gcaataatca cttgtacgac
   118681 ttgtgacaac gctatagata tctgatccac caactctttt gtcgattctg aacgtgctcg
   118741 aaaagctagt cccaacaata cagcccagaa taagacgcca aggtaattcg catcaataag
   118801 agattgaaca ggattttgtg tgacattcgt caacatagta gataaaacac tttccaaact
   118861 ttgcggtgct ttttcagata ctgtttcttg taaagtaata ggaacagtaa aaattgaact
   118921 tacaataact gctacaagtg cagaaagcaa tgttcccact aaatataaga tcaaaatagg
   118981 cttcacgaat gtcttattcc caattttatg cttggcaatt gacgccatcg tcaagaaaaa
   119041 aaccagaata ggtgcgattg ccttcaagca actgacaaac aacgttccca atattgaaat
   119101 aaatgtccat gtggggaaaa agataccaaa caatattccg actaaaatac caacaacaat
   119161 acgttgaact aacgacatat ccaaaaactt ctctaacaaa tgaccaccct ttcacaattt
   119221 taacaatcat tgtatcattt tatgcaaaga aactaaaggt ataattttct aaaaattcta
   119281 acaatacaaa caaccatttc ttgctaattt tgtacttttt ctgtctacac gttgtttttt
   119341 gagatattct aagttaggat gttattaatg ggcaacttat caggaaacgt tttcttatgt
   119401 ttttgtccaa tttttttgtg aggtgtttat tcttgaaaaa aataaagaag gagagttatg
   119461 gcttcctgac aatctttgtc gcttgtataa tgatggtttt acagcgtata ttgatttcag
   119521 cagtcactat tcctacatat gcaacaaacc ctggtcgatt ctacatctat acatttttac
   119581 aaacttccgt tgttattggt gcaaatttga tcccacttta catgggttac caatatcaaa
   119641 aaatcgcccg tttaaaagtt ttacattatt taagtcgttt tgcctttata ttcctaatag
   119701 ccacactagt ttgcaatatc tttttttacg tacaagctgg cagcttgaat attcgtgatt
   119761 attggcttat cctaacacct atcagtcaga attactttac ttatgctgtt tcttgtactt
   119821 tgcttttttg tagtattcct tatacagttg gatttttaaa taaattatcg aaaacaacaa
   119881 ttaaatatct actattattt ttaacaattg gattagttgg ctgctctact ctatttaata
   119941 aagacccgtt tgcattacag aatggacaga gtattttatg gattttttat ttatttttaa
   120001 tgggttatgg tttgaaagaa tggaactgga aagaaaaggt ccgctataag cttcctcaag
   120061 tatttatcag cttgattgtt ttattcggtt tgattatttt gatgacacaa atttcactca
   120121 tcatcagagg aaatacagat acagcgctga ggttttctaa accatattct ctcttcgcaa
   120181 tgtattatac tttctcttcg tttttatttt tagaaatgct atctgataaa ttaggtataa
   120241 aaggaagagg aaattttctt gctgtatttt tgattgtcac tcaagttata tgtaatttcc
   120301 ctttagtttc ttatcgcgta tctacattta tgaaacgtga atttccaaac tcagggaagg
   120361 catggctgat aaatatcggg cagtttttta tttgatattt attagcagtg tccctcctag
   120421 ttatcttttt attttatata caaaaaatcc gtttcattca tcgtttcatc gaatacttcg
   120481 cttttgattc gattgaacaa ttaactcagc gttggaaaaa aagaattcat tggctttttg
   120541 ttcataaaag ttatttttta gttgcagcat ttttttactg ctttaccttc gtgcagattt
   120601 tcgttttaga accaaaagaa ggttggaaag aaactatcca ggttgcctta aaaatattta
   120661 ctcagcgaca agcacccatg atcctgacaa tcatcattat tatgggattt ttcttcttac
   120721 tattgttgat ttccaatcgt ttttggtacg ccttgactgc taccttgatc attaacttac
   120781 tgctcactat ttcgacagta atcaaaatgg aaatgcggga agagcctgtc tttccttcag
   120841 atttaaaaat gctgaccgga ttgtcagaac tattatcgat ggttagccca gtcttattaa
   120901 tcgtgggcgg attgatcctt ctcctattat tgattactag tattattgta caacgtagac
   120961 tacaaaaaca gtattccttg aagattcatt ggaaacgtcg atttatcagt atcgctgtat
   121021 tattaggatg tttttcgggt gtctttttca ttaatcacaa aaattcgcct tctttcttgc
   121081 tgtttaattt atttaaagta aacaagactt tctttaacca gcaaggcgcc gtaaaagata
   121141 acggaccagt cattcaattc ctaaacaata tcgacattaa aatcatggaa gaacctgctg
   121201 gctattcgaa agaaaaaatt aatggaataa tgaaaaagta caacacaaag gcagatgaaa
   121261 tcaaccaaac aagaagtgac tggttaaaca atcagacgat cgttctcaat ttaagtgaaa
   121321 gcttcagtga tcctgcacgt gtccctaatc tgacagtacc tacagaccct attccttaca
   121381 tcaaaagcat tataacagaa ggaacaggag gattgatgtt atccgtcggt tatggtggcg
   121441 gaacagctaa catggaatgg gaaggactga caggcttgag tatcagtaac ctgtctgctt
   121501 cgttagttac accttatacc cagctagtgg agcgtcagaa aataagtcca aatattaccg
   121561 atctattcga tgaaaaaata gccatccatc cttttacagc tgctctttat cgcagaaaac
   121621 aagtgtttga taaatttggt tttgacaggt tctattattt agacagtccg gataaattaa
   121681 cttatacgga taaaatagaa caaagcccac gcatatcgga tgattcagca tacaaagaaa
   121741 ccttgaaagc tttacaaagc aatacggaaa cgtcacaatt catccaatta tccactatgc
   121801 aaaaccatat gccttatgat gattactata gtgaattgaa ctacactgct gaaggaactg
   121861 ctgtcattcc taatagaaga aaagaattac aaacttatat gcaaggcctt cactataccg
   121921 atacagcagt aaaaaaattc atccaagaaa tcgacaaaat cgataaaccg attacctttg
   121981 ttttttatgg tgaccattta cccgcaatct atagtgggaa tgattcgaaa aaatatggat
   122041 tagaacagca ccagacagat tatttcattt ataacaacaa gtatagtcgt gaacaagcag
   122101 ctacgttaaa taaagaagtt gttgctccta ataactttgg tgctatggct cttgagcatg
   122161 cgaatatcaa agtaactcct tactatgcgt tgttgacaga agtatctaat catctacctg
   122221 cattaacaat tgatcctaca gaaagcttgt ctaatcgttt taatggaaaa caggtctttg
   122281 ttacagagga tagtcaaatt atccaagaaa aaaatttaac tgagaaacaa aaagaaattc
   122341 tagaagatta tcgtctgata caatatgatc ttgttgcagg taaccagtac agtgcaacat
   122401 gggcgacaca aaaaataaaa gactaacaaa taaactgagc aaggggttgt gataaaagac
   122461 atgtcacaac cccttgttta tctcattaat ttgcttgtcc ataaacgatt tgattaaagc
   122521 catttttcaa ttcattattc gctgtctcgt tgaagtaaag ataattatcg ggattcccct
   122581 gattttgttg tgtcaccaat acttttggct gattttgctg aaacccgaat agatagacgt
   122641 gctttttcag atatggttct gtctctgcat cggaatagac ggctgtgatt tgatagtctg
   122701 cttgacctgt cccactttct gaccattctg cagaaacagg cactccacca atagccatct
   122761 tccactcccc gttcaaaata gcttgaggga ctttaagacc atataagtcc acactcattt
   122821 gattcgtata acttttgtat tgttgtccca ttgtttggcc ccatttggtc ataaatgcct
   122881 ctaattgtgc ggatttgtct gcattccaca gtgtttgttc aacttgttcg ctgctttctg
   122941 aagaggaaga tgaagcctct acagaagaac ttgaagtaga tgtttgagtt gtttcagtcg
   123001 ttttggaaga ctctgacgaa gtagaggtat ctgctgtttc tgtacttgct gcagttgact
   123061 tgctctctgt gctagttgtt gtagtagttg caactgttgc tttcgctgtt tcttttgttg
   123121 attctttttt cgattcattc ccgttggtac aaccagctaa caaaaaagct agaccaattg
   123181 ttactaaaat cctttttttc aattaaaaaa ctcctttcga ttactcccac ttctttattc
   123241 tatcaaataa gcaatgtgat tttattgatt tttcgtattt tctcatcaat ctttcaaatc
   123301 ttctaaaaac tgagaagcac ccgctgatgt tgtctgtaat acatcagcgg gtgcttcttt
   123361 tattttaatc ttcgtgattt tccaatttac ctcacgcttt atatttgtat ttgaagaaac
   123421 atagactttt agtcatcatc gtcttcctca tccaaatctt gtcggacagt ttttccttgg
   123481 agtctttgta cttggtagat gatttcttta ttgaaactta aaagttcaac aagcaattgt
   123541 tgcaagccat atttttcttc tttttcagct aatttgattg cccgtgtgat aaagacattt
   123601 tggaaatcaa aatcttttgc aaagttttcc aacattgttg aggctggtaa atatttgttt
   123661 tgaccatgtt cgtaaatttt tccataacgc atgacttctt ctaaagtaga cggaacgatt
   123721 tcattttcaa tcactaactc atgagagatt ttattgaacc atctcgcttc ttgatcagca
   123781 tattctgaaa aaagggtact taattcttgc gaagatgtac ccaaaacata caattgtgct
   123841 tggatcaatt tcatttcttc taatttaaaa tttgctaata catgccccac catggcgcca
   123901 gcagttggtt tatgatggtc gatatctgct tgttttgttt cttgaacaaa tttttcttcc
   123961 actatattca ttgatttacg cctcttttac ttttgctgat tggacttcat aacctaattt
   124021 ttccacaact gcagctaatt gttcagggtg gacttcatct ggatcaaagt ttgctttgat
   124081 tttgctcgca ttaaacaaaa ctttcgtatt gcttacccca ggctgttgag aaaccgcatg
   124141 ttcgattttt tgcatacagc ttggacaagc tagtgtatct aattgcataa taattttttt
   124201 catcgtagtt cttccttcct ttttcttgat agtcttattc tagattacag cttccataaa
   124261 aaaattgatt cttatcaagt tttacgattt ttcttctttg ctttcgtgtc caaaatacaa
   124321 taaacgcatc gcgttaaaga taacgatcaa gatactagct tcgtgaacga acatcccact
   124381 agccatgtaa ataaacccgg caaataagcc gttgagtaaa aataagacga caccaatggc
   124441 aatcactaca ttttcacggg tatttaaaac agttttctta gccaagcggt aggcatgaac
   124501 caacgattca aaattagatt gcatcaagac aacgtcactc gtttcgatag caacatccgt
   124561 tcctgatccc atagcaatcc cgatatcagc cgcagccaaa gagggagaat cattgattcc
   124621 atctccaata aaagcaacgc gcaatccttg ttcttggtat tttttcacaa attgaacctt
   124681 ttcgtctggt aataattcag catggacttc atcaatgcct aacatatcag ctacataatc
   124741 agcagtcatc tgattatcac ctgtcagcat cactaactgt tttgctcctg cttgtttcaa
   124801 cttagctaat tgttcagcga cttctggtcg aatcacatct gacacaccta tgatttgagt
   124861 gatttttttg tcgattgcta caataatgac cgagctgcct gtttgttgta attgatgtaa
   124921 atcttgtact tgctttggtg tgagagaaag tttgtgagcg gcaatcaatt tggtgttgcc
   124981 cgcacaaact gtatgaccat caatctcagc tgtgattcct tgtcccttga ttgtctgatt
   125041 atctttgact gctaattgtt gaaaatcaat gtctttctcc tgagcataat ggatcacggc
   125101 gcgccccaga ggatgatcgg ataacgtttc taatcgagcc actaaagcta agctgtcccc
   125161 ttcatcctct gtatagttct tgatagtgga tacagcagta gttcctttgg tcaatgtacc
   125221 agttttatca aagacaaaag tatcgatttt ggaaaaagta tccattgctt caccgccttt
   125281 gactaagatg ccccgtttcg ctccattacc gattcctgct acattcgaaa caggcgcacc
   125341 aatgactaaa gcgccaggac aaccaagaac caaaacagtg attgctaaac gaaaatcacg
   125401 actaatcaga ccaacaacca acgcaataag taaaacggtt ggagtataat actgagaaaa
   125461 tcgatcaatg aatttttccg catgggattt ggtatcttgt gcttcttcta ctaattcgat
   125521 aattttggca aaggtcgtat catctccgac cttagttgct tcaatcgtca cgtagccatc
   125581 tgctaaaatc gttccagaga agacttcatc tccactgatt ttgacgactt gtcgtgattc
   125641 accagtcact gcggcttcat ccgcataccc tttcccgtca atgactcgtc cgtccacagg
   125701 aatagctcca ccagttttta ctaagacgat atcgccttct tccacgtcat cgatttccac
   125761 ttcttcagtc gttccatctt cttggacaac actagcagta gatggggcca tctcggtcaa
   125821 agccttgatt gactcccgcg ttttggtcaa tgttttttgt tccagatagc taccaaacaa
   125881 aaacaagaat gtaacgatag ctgactcctt gtattctccg atcataaagg caccaagcac
   125941 agcaatagta acgagtaaat caattgagat aactttgact ttcactgcct gatacgcatg
   126001 gatcataata ggtatgaccg caataataga agctatggct agagcaatgt tatagaacaa
   126061 gacaaaacca gtaaattttc cgataaaacc aaaaataatc aacacagctg ataaggcggt
   126121 gatatgattt cgatgctctt ggatccactt ttgtaatttc atctttctcc ctcttttcct
   126181 ttcattctta cgagactaag tatagatact tatccatcat aaaaaattga ccactatcaa
   126241 gtttagtcaa aaaggcggtt ttcttttggt agaatacact acaaaaaata gttagcaaga
   126301 agactcacta aaagtgatct tgccaactat tgtcgtttct atcgaaattt tcattgaatg
   126361 attttcctgt acagcataca tcggagatct atttctttat ctaagatttt tatcttgcta
   126421 attttttcct ttagtctttc aaatccacgt cgttcataga attgatacgt acagccacta
   126481 tctgtaaaaa ggaatatttc ttttcctttc tctctttttt caaattctgt tagcaacttg
   126541 gtaccgatgc cttttgtttt caaatcagga tttgcagcta aaaaaatgat ctctccatct
   126601 ggcgaatgct tctttaaata tgctgcaagc atttgttcat ttgctgtctc gtaagctcct
   126661 ttactttctt tagcaaatat attttgtaaa aaatcgaata tcttaacata tatttgctgt
   126721 ccaaaagaac gatgtttctt tttttctcct tttatctccg ccagcaaaac acctgccaac
   126781 tcgtcctctg catacaaagc aagaatttga gttgctcgat tcaactccat atacaaaaaa
   126841 tatcttccgt aaagattcag cagaagttca ttttccatat atctctcaaa gttcattcct
   126901 tcaacagcaa aatagatagc ttttttgtaa tctttctttt ctaagggttt aaaagttagt
   126961 ttcatcattt cgcctcctca acttcaatga ttgtgttata cttacattat aatagacaca
   127021 gtgtctacta tctataatca atcatctata agtttgtcga ttaagcgaca gctttgcttt
   127081 gatatgtcgg ataaggagat aggtaaatga aaaagacaga tagacgcgtc aaaaaaacag
   127141 aaaaagcttt ggctgaaaca ctatccacat tattagtaaa taaaaagatt caagcaatca
   127201 ctatacgcga actaacagaa acggcagatg tacatcgttc gactttttat acacattata
   127261 aggatatcta tgatctatat gatcaattag aaagcaactt ttttcgggat ctaaatgaaa
   127321 ttctctctta tgatccagcc catagctatg aggaactata cactcgcttg attgactatg
   127381 tttacgcaaa tactttttta tatcgcttat ttatcctcaa ccatggtgat atttcgtttc
   127441 agaagaaagt taaacaagtt attgaagaaa actatctgaa catatggcta tatgaagaca
   127501 agaaaacgat tatcacagaa gaaatgagat tctttactac gtaccacgtc caaggttgtc
   127561 tatccgttct caatcggtgg gtagaaagca attacaagta ctccaaaaaa gaattattag
   127621 aactcttaca gaaactgaat gatcacatag agaaaatcat gccttgaata aaaacatact
   127681 aagcttagtt aagaagaacc gtaaccttct cgtataacaa ctgaatctta tttagccaaa
   127741 ttgtttactg aagatttttt gagtaaaatt tctataattc ctacttattg aaaagtagtt
   127801 tgtagtgctg cttctccctc tctaagtgat atatcttgtt gtaccaatca aattcacctc
   127861 atcattaata aattactttt aggctaattt attacaactt ataggttgaa gctgtaacca
   127921 atttatagtc atattatatt tttatcatcc agtttattga attcttctcg tttacctcaa
   127981 ttcattttca aggggaatca gctcatcatc taacatttta cgccatgctc gcttcatttc
   128041 ttgtgggtat ttaaatggtt tcgcttctgg atgtttggct ttatatcctt cgtaatcctt
   128101 actgttgata attgctttca tattaggaat tggatcttca cctggcatat attgcagatt
   128161 agaagcatgt aattttttct cagaaacaaa ccactcattg gaaaagacat gaatcaaatc
   128221 atcacttact tgtgtaaaat agcgacgttt aatgacaaag atatcttcat tgctatctag
   128281 tgtaccttca tttctttgtg cttaaattca aagataattt tctcaataaa gatttttgat
   128341 tccagtaaga ttcctttact ccttttacaa catagcgttg tcctttgttt tgaaacatct
   128401 tcttcccctc tttgccatta aaaatagccc aattctctat aaagaagatt aagcttcatt
   128461 attataatat aaagtttaaa tttggtttat tacttacttt ttcttattct ctagtaagct
   128521 aatattgaaa aaaatgtaat cagatcatta tttttatatg ttgtaacttc ctgataagaa
   128581 aactaattaa aagaaagagg taactacata aactaattaa aaaagctaac ttcatagttg
   128641 gaggataaat ggaagaaact tcaatgattg tttctccttg tgttccttca atcgacacta
   128701 gtcctattat caatctctct tttttgtcca tttacttttg cgactgttcc ccaataatca
   128761 aatacaggaa gcgttatttt ttgtccttta gattcatgta ctgtatagac tattttcgaa
   128821 tccatcactt gataaggcat agtatgccat tccttgccaa ttcttacttc atgattctgc
   128881 aaatgttctt tgtattgtaa tgcttccgtg ggtgcatagt cataaagtcc tccgggaagg
   128941 agtgtcgtgt aacgaataag attttcttga ttcattacca agtgatctct ctgagtgaca
   129001 actttatgtt catagtttgc ttttgttgca gcagatacag ccaacagtaa taaaacaatt
   129061 gccgttatat taactacttt tctttgctta gctgatgtgc tcttcatgaa aacgaacgat
   129121 aaaatcaacg agccaaacag tacttgaaag cctaagattc gccaaggaaa ttgaatcatt
   129181 tgaatgggtg tattttgtaa taaagaccat ggaaaaaatt tggtggataa gagccaagag
   129241 actaccgcac taaggtaaat cactttttct ttgcgattaa atcctttaaa aaagaagagt
   129301 ggacagatca aagaaataat accaatgatt cctaccgtaa aggttgtcag gtcattattc
   129361 atcgcaccaa tcaaactatc cgagacattt aatgcttggt gttgtaaggt cgttttatct
   129421 ggctcattaa tcgattgaac catgatttgt cggaacatcg gataccaaaa gtatgctgtt
   129481 aaaagaatcg tcattcctac tgcctttgac aactccacaa tcaaccggat gctttctttt
   129541 tttcttgttt cacttactac aaagccgcct aagactagta agccaacggc tattacactc
   129601 ataaaagtgg aaagaacatg tgtataaatg agtaagctca tactgactgc taatggaatt
   129661 gcacatggct ttttctcata aatcagtgcg taagcataat acataactat cggtataacc
   129721 gtaagtgcaa tcgcttcagc aatagcagaa cgatgataaa tatcaatcgt acggtaccca
   129781 taaaatgtat ataaacagct aaatataaaa gccgcatcga ttctttttaa aaattttagt
   129841 ccataatgaa acatcagcat gatagtacaa acattcaata gccaaacata taagatataa
   129901 ctaacgatta aattattact tatccaataa aaaatgactg ccggaaaaaa ggtcaagtag
   129961 ggataaaaat aattcactcc gctcccataa tgattaaacg tcgtaaaatt gattggacca
   130021 gaaaaaattg aagataaccc tttgactcta gcaaaatgga aggcaatatc ccccgatgta
   130081 gaaatagtat gaacatttct aaaataattg attgaataaa gtccagcaat aattgctagg
   130141 agaatggaca atattatttt tttctttact tgcattactt aattatatac tcctttcatg
   130201 gattgcttta tctagtataa cttaaatttt aaataaatcg ttatttattt ttactctcac
   130261 ttaaaaaagc actaaaatta taagagaggg aggtaatggt ctgaacacca agaattgttc
   130321 caacatcgaa ttgcttaact aatgagttca aagcttaatt atgtggctct tatatttacc
   130381 gagcgtggat tgcgatctct ttagcgaaac aaacatcttt cataaaaaaa acagccatat
   130441 ccgctttctt ggatatgact atttactact actttactat ctttcattct tcttgtctca
   130501 cttatgataa ggcgctgcgt atcatgagtg aatgatatta ggaaaacttc ataaaataaa
   130561 tcttttatac atttccactt ctagatggtc attcatgtta gaagaatcaa actatttttt
   130621 tactgtcgta ataatattaa aacactagaa caaatcaatt aaattccata aaatataaat
   130681 aatagaccta gcatcatctg gtataaaatg gatgctaggt ccgttatatt tactcaaaat
   130741 tgattctacg aatagatctt cttatcattt ctattttagt agtcatgcgt tgttgttctt
   130801 ctaaaggtgg taatggtata agtagtttag ataaatttgt accatttaca tttggttgcc
   130861 cagttccgta agaaattttt tctaataact tccaatataa tggcgagtct aagaagcagt
   130921 ctacgtattc tgataataat ttctcaacta acctaattcg aatcaagtaa gaagcaaaaa
   130981 cagattcctc acttatttcc ttaaccaaga aactttttcc catagttcct cctgtccttg
   131041 ctatcaaaat gtcattttct tccaatctta aatcaactag tttagaatta ctaatatcac
   131101 agtaaggcac actggaccaa tttacacgtc cctcttgtat atctgtgatt ctcaaaaact
   131161 taacattgcc ttgttttttc gccgaatctg tatagccata ttgaatctta gtagaaatac
   131221 ttcctaaata aacccatagt cgaacatcgc tagatgattt ttcttgtcgt tctttatgaa
   131281 caacagtgat catcttgagt tgtccgacta ttttttagtt gcagcaatct ttttgggacg
   131341 cttatttgca tgaagcaacg ttgctaagtc tagtaataaa acattggcta acacaggatt
   131401 ggctttctcg cgatacgctg cggcgccact ttcaaacaat actgctagac gatcttctac
   131461 aacaacgatt tgttccaaat acggtggaaa tttgattcgt tttaggatcc ggctaggaga
   131521 gtcccgctcg gacactaaga gttccgaggt ataaggacca taagaacgac tggctaagag
   131581 ataatggcca taaaaagcca caccttgaat cttatcagat gtttttctgc tcattgtcac
   131641 ggttggttgg attttgtctt tgcggcgaat tgctgttttc atttcagcta tcgtcggcaa
   131701 tagatagaaa gtcaactgtc cttcaccttt tagtgtgaag ttgccagata ccagttgatc
   131761 attgttttga gtcaaatacg atgcttgtgg aatctctgag agatcagttg tcagccaata
   131821 agcgattggc tgtcccgtct cttcagatgt atctgctaag atatcgatca aacgtaaagc
   131881 ggctactcgt ccagtagcag atcctgtgat tgtcaaccat aaaagttccc gttttgtatc
   131941 atatactaag ccccctacat gtggccgttt aggtaaaatc agcagtttga tcggctgtcc
   132001 tgtttctcta tccagtacat gaataataga atgatgctga tggtcatagc agtaggcaga
   132061 aataaacaca aaatcttgag aaaaagccaa cccttgcggc gtcatccatt gacaaacctc
   132121 attttcgccg gtttccggca ccaccattct tgtttctgtt aatccaggaa tgttatattg
   132181 ttttgcttca gccttttccc aagctgcttg aacaatagga taccgtttta actttttttc
   132241 aatatcctga tgtatccgtt gctgcttcca atgcttaaac tttaggacat agtaaacaag
   132301 aaggccaatg accagcaaca ctaaaatcat tattgctacc cgctgtgtca tttttttgcc
   132361 tccttgttct attcgatcaa tcgtccatat accgctatta attaccgtaa ctacatgttt
   132421 cttttactag cgtttcttta taggatcttt cataatctca tttatagtaa cattaagaat
   132481 cccttattct actatagtca cttccacatc aatattgcct cttgttgctt tagagtatgg
   132541 gcaaaatgcg tgggcttctt ctgcgatttt ctctgcttgg tccttctcaa gtcctggcaa
   132601 agaaacttct aaaacgaccc cgatttggta gcccccatct tctaattgaa ataaagatac
   132661 ccgtgctttg acttcagaat cagactcaag attattttct tccagcatgt gttgaaccgc
   132721 gccgttgaag caagatgcgt atccagccgc aaaaagttgt tccggatttg tcccttcact
   132781 atggattcct gggcggtcga tcttcatgtg catacttccg tttggtgcct caacttcgcc
   132841 atcacggcct ccacgattga tcattgctgt ttcgtacatt tttttcattg agatcatcct
   132901 ttctttcgtc gttctatatt aagtgtaacg agtatttttt aatctgacaa aggtaatgct
   132961 tgcattcggc gacatcagtt agacaacatc gatgatcagt gatagaagca gtttatctat
   133021 acaaaaaaag aagggttcta tgatatttgg tgtccttact cgccttgagt aacaacacca
   133081 aatatcatag aaccacttaa tcagcttcac actgcttttt ctactttcgt agttgaaaaa
   133141 aacttaaaca aatcatcggc aaatagttaa agaaaaaact taatcgttga aagaagccca
   133201 attgcttaaa gaaggcaaac tgactaatcg acggaatccg tgctaatccg taaagacctg
   133261 caattagtaa actaataact gttaaaacaa gataaaaatg gctaagcgta ccttgaccac
   133321 tttgacgata aagcagaaca agtaacaatg ggaaaagtag aaagcctgca tagcctagcc
   133381 cagaacctat attatggatc caagtcgata gattacaaga gctttcattc gtattgatac
   133441 tgaataagcc agtaaaaata caatcaccta tcccatataa accaactgtt gcaacaacaa
   133501 gtattgcaag tgtttttgat gttccaacaa ataggtgata caacgcaggc aacgaaagaa
   133561 caaaaaaaag tccagataca actgaccaga ccaagaaagc acgtctgact ggactatcta
   133621 cgtcaccaaa cacactaatt acggtagtca tttgattcag tttgggataa aaaaggccaa
   133681 ggatatacgg tgtcgataaa tcactcatca cacctaataa taaaaaataa aaaccatatt
   133741 tttgcaaaaa aatcattttc ttcccctact ttttagtgat tattcgatac atccaacaat
   133801 caaatttttt ctcattttaa cacatatttt tattctagtt gtcttttgaa caatcaataa
   133861 cataaaggtg agtttcttct acattttaaa aaatcgaatg gcactgaaga gttcagttgt
   133921 cagcatcaat ctcctactca aaaaggcaaa aaaacgtaag tcagatgttg ttgacatctc
   133981 acttacgcat ccattgattg aaaatttctt agtaaaatct actagcctaa aaatttgaag
   134041 acttcttctc gatcatacag tttaaatact ttctcgcata aaaattgagt aattagtgcc
   134101 gcaaaaacag ggatgactaa ccaacaaaga acaacaagaa agccatttag ccctgcatct
   134161 aaagaagcca acggaccaac aagtccgacc acgccgaaac cagcagaagc aggcgtacct
   134221 gaaatagaaa atagagcgac aggaatagct gtaatcactg cactaactaa acaaggaata
   134281 agaatcacag gttttctaaa tagattaggc atcatcatct tcatagcgcc taaagcaatc
   134341 gcaattgtca cacctgactt attaaccttc caagagttca ctactaaaac aaaagtggtt
   134401 gcagctacgc ccatcgcagc cgcaccagca gaaacaccat ctagttggat tgccattccg
   134461 attgctactg ttgaaatagg agagataatc aaaaaggcaa aggaacaacc aatcaagata
   134521 gacattaaca gcggttgtaa ggtagtgaag ttattaatca atgatccaat tgcagaagta
   134581 attgctgtta caaaagggaa taaataaaaa ccaagtaaac ctgcacctac acctacaaca
   134641 atcggtgtta acacaatcga cactgaccca aaactatttc caatcagtaa aatcagcccg
   134701 acagcaatcg atgctgtcag catcgtatta ataatatcgc ctgtaccagc accaacgtaa
   134761 acacccgttt cacccaaatc aggtgtgaat ttgactacac cagaaccaac aaaagaagct
   134821 ccggctacaa ccatcattct catcggatca aatccaaatt gtaaggcaat caaaccacca
   134881 atgatcaaag gtgttgctag ttgaaaaata actgctaact gtgcgatcgt taccgcaaag
   134941 ggatacgcac taaaatattt taaaatggca cctaatactg cattaggaat caatccaata
   135001 atgattcctg ttgctgtacc agcgaggact ttattcagaa aaattttcgg tgatattttt
   135061 tgtgaagttt ccattcaatc tctcctttat tttacgttca aactatcttc ttttgcatgg
   135121 acaagctcag tcaagaaatc acaataaggc gtcgcaacac catatttctt cccttttcta
   135181 gaaattgcgc cattgatata atcgatttct gttaatcgat ggttcttaat caagtcttga
   135241 tacattgaag gataatgtaa cccaattgtt tccgggtcaa aacaagattc acaatgtgca
   135301 atgacttctg ggacatctag ttcaatcttc tctactgctg ctactttggc aaactcattg
   135361 acaatcgtcg ccaccatttt atgagcagtc gatgtttttc ctagctcagc catattaacg
   135421 tctaagattg tgcatagtcc gttcattgtt ccgttcacac atgctttgcg ataaatagaa
   135481 tagtgaatat tgtcagaaaa atgagcattt aatcctgatt cagacaattt gtctgctaat
   135541 ttttttgcag ctgcttcttt tccatcacct aaattttgta attctactga accacttcca
   135601 aataatttga cctgaccagg accttcaaga cctgccgtcc acatggtatt tccaatatag
   135661 atattttcca ttggtacaaa tttctcaata atatcttcat gcccgatacc atttaataga
   135721 cataagacct ctgtatcttt tttgattaat gattggatat cctgcagcat tttttccagc
   135781 tgcatcgctt tggtaaatag aataatcaga tcaacttgat cttctttttc tacttcggat
   135841 tgaagaacga ttggtagttt tgcttctacc tctttaccat taaaatttgc ttgtaatcca
   135901 tgttctttga tttgttggac atgttctgcc cagccatcga ttaataatac ttcgttccca
   135961 ctttggtgaa gcatcaaccc aaagcgactt cccattgcgc cagcgcctgc aattgctatt
   136021 ttcattgatt cttcctcctt tttctttcta ttatactcat ttgagaatct tttcacaacc
   136081 tcgttttgtt attcttctct tatttatcat gtttatatcc accattcaag ttattttatc
   136141 agcaaatcaa taaaaactgg aatagaatcc caatttcatc gtaagagaaa ctagactagc
   136201 aataagaaaa tagaaacatt catagaaaaa atctcactcc tctgattata aaagaggggg
   136261 cattaacctc gtgtatacta aaaccttcca ttcattcgat ggatgatttt ctttttgtga
   136321 aaaaatattc attgtagtac gatgttgcgc aacacagacg gatggtattg cttattattt
   136381 attaaaatcg aacaatacac ttatttaaaa acttctttcc cagattttct tccattttta
   136441 cagttataac tagtattatc atgaaaaata gccttttatt gtctatggga ataataattt
   136501 aaaattggta cttttttatt aattagttca tcgcccaata atctctccag tttcttagca
   136561 tctcctgact caaccggaat tatccaagcc cagtctaaat aattttttaa aatctcatca
   136621 tattttgcaa atatttcatt gccatattcc gtccttaaaa ttatattttc agtgccagac
   136681 aagtgattta tattgagttg ataaaaaaga tctccatcta aaaaaatatc attagaagaa
   136741 gatttcccca cagtaatgac cattccatca gctctatgtg ttacatacaa ataattgtat
   136801 gacctcattt cacctaatga atcaattatt tcttgccctt tagctctaat atattgatct
   136861 ctattaccta tctcactctt atttaacaaa agttttttca tgtcaaattt aatcggctgt
   136921 tttatttttg taaattcgct tctactacta atccaatccg agattaattt ttcaacatgt
   136981 cccagagtaa actctgattt attcgctaaa tacttttttt gttgtattaa agacatcttt
   137041 ccccgaagct caaaattgag cctacatcga ttctctatct cttcaaaaag catcttatct
   137101 tcatcattct tatattcatc attcataaaa ccaatccagc tcctcgatta agataatatt
   137161 ttaatctcat acaaaaacta gctctcatca aaacaaccat tgttttgatt tctcatgaaa
   137221 tttctttata cttttctccg attataaatt cctaacccct tgagaaaatt acgcatatat
   137281 ttatctccgc aaggcttaaa atttcgttgg ccttcttttc tcaaaaatgc cccgatttcg
   137341 ccttttgatg gatagatttc tgcctcagca aaaacgtcta aaatatcatc agtggtcata
   137401 gacaaggcga tttttaattt ttttagcaac acattgttga tatatttgat ttccttgctt
   137461 ttttgcggcg tgtctactat cggcttatct ttttgcggac cacgcgccaa gagaatcagg
   137521 ccatttagga atgcgtcaaa aacttggttg ttgatggttt tttcatacac attattttct
   137581 gcattatctt tgttcttatc ctgtactttg gtcagtaccg cctgagcttc ttctttagtc
   137641 agtggtacac cgcctagctc aaaagcttta atcaaatccg tatctttcaa atcaatcgca
   137701 taacgcaatc gcaataacct atcattgtta ttcatttgtt cctccgaagt atttagtctt
   137761 tactttctta cgtacttttt gtcttgctaa gatttttagg atcaatgata aaaaccgcat
   137821 cacttatgat aaggcgctgg gtatcatgag tgaatgatat tgctgaatac aaatagtccg
   137881 accacattaa gttgatcgga cttcataagc attttattga ctaatacttt tttgcttaag
   137941 aattgattct aacatctttt gctaaagtta agcatcctat agttcctgca ttatctgcta
   138001 acttaggtgt gacaatgtat tcttcgatat ttggcgtatc gacatagccg ttaattagtt
   138061 ctaaaaaacg tctttgtact ttctttttca gatgttcttg ttgcatgaca ccaccaccaa
   138121 aaattataat atctggtgaa agcatcaggg tcgtattaca ggcacattgt gcaatataat
   138181 ccgcctcgat ttcccagaac ggatcttcta tagaaagatc ttgtcccttg gttcccgtac
   138241 gcatctcgat agcgggtcca gatgccaacc cttcgagaca atcatgatga aaaggacaag
   138301 taccagagaa agtatctcct gaatgtctct taactaacgt atgccccatt tctggatggc
   138361 taaacccttc aacaaaaatc ccatcttgta tagctcctcc accaattcca gttccaacag
   138421 tgtaataaac aacactggat aaaccttttc catgacctga aacatactcc ccgtaacaag
   138481 cagcattcac atcggttgtc caggcaattg gaatttcgaa atgcttattc aacgttccaa
   138541 caaaatcaaa atttttccag gcagtttttg gagtagaggt gatatgtcca taggtctttg
   138601 attcacgatg gatatcaatc ggtccaaatg accctactcc aatgctttct aattgttctt
   138661 tatagtgatt aaagaaatca attaccaaac tcattgtttc attcggtgta gttgtaggaa
   138721 aactcactct ttctaaaacc tttaaatcat cattgccaat ggcacaaaca aatttagtcc
   138781 cacctgcttc tattgagcca tacatttctt actccaccgt ccttttatct aggaattcgt
   138841 tcttccctgt ctaatagttt ttcgaaaaac tcatgtggtt ccttttggta ccaataggca
   138901 acagaagaaa tatcgtcttg acgctcataa agcttgatgt catcatttcc tagggtttgg
   138961 aaagtaacac gtaaattttc tttgaaacta atggggtcgg gtaaatgcca gcggtacaac
   139021 ccatgcatcg gtaaagcatc atcgccaaaa ccatgaactg gatttggatt aggttttcca
   139081 gcactaaaat aatcccgggt gtgatctctt ttagattgaa aaggatagcc cataaatagc
   139141 gtattaaaat tttgagcata tgccctttct ccaaagttct ttttatggaa agcccaagcg
   139201 ccgccaaaat aatcttctgc tcctgtagat gttattgtag ggaaatcttc atctccatct
   139261 atataaaact taaattctcc ttccccccac cagtatcttt ccaatgcgca caaagcaaag
   139321 taggttccta cataataccc agtgcctttg attccatcaa taagggtgta atcttttcct
   139381 tttgtcgtga tgggttcacg gttccattta gcatgaaaat acatcgcatc atccggtact
   139441 tcagtcaatg cataattaaa tgtataaaaa atgtgcttaa taaactcagg atgttcattc
   139501 gttaaagtaa tcttagcttt ttttcgaaat ggcatctcaa aatagcaatt catccctgat
   139561 gttggattca ctacgattgg taaggaattc acttcgcatc ttgtcccaaa cccattacaa
   139621 aaaaaatctc ctaatggact ttctacagaa ggagtggatt catcatccca ataaatacgc
   139681 aaaattaaat cacgtaagac gaaactccca gcgattgttt tgtctgtcac tgtcatccaa
   139741 aaatgacgta aaattccagg gccttccatt tctgctaaag taattgtctc tccagcattt
   139801 atcggaacag aaggtgaccc ttttctccca attcctagat gactcgctgt cattgccgct
   139861 ttcccttttt caccattttt attttctggt gaaatagttc tactttcctc tcccttgtgg
   139921 agccatatat tatttaaaca attcatttta ttcgtcctct atcctttctt cacttttttg
   139981 attattcata tagatgattt gttgttgtgc ctccgcaata attgctccaa tatctaaatt
   140041 atcatttcct aactgcaaag cgattgacat tgccaaggga agattcattc ctgcaattac
   140101 gtgcactctg tcagaaatat aggtaaaaaa cttttgattt acacttcctc cagccatgtc
   140161 agttaaaaca attatttcgt ctgtagtatc cactaactct ttaatttgtt catctatatc
   140221 atcaatttct tcgacatatg cacataagct actaatttga ttatgcatac ctgttaaaaa
   140281 ctctagtgtc tctttaaaac cctcagctag tattgaatgg ctagcaatga tcaatcttct
   140341 cataattcaa atccttttcc tttacgctaa tattccaaat gcagccataa ccatcgcaaa
   140401 aacaataacg agtagaatca aaatggtcat actgattttt ttcttactga gtagataata
   140461 tgctgcagca gtaataatta ctggaattaa tgttggtaaa attttgtcca acattggttg
   140521 gattttcata ctaacttcac caaaagtgaa tttaagtggc gttgaaacac tgataactga
   140581 agcaattaat gatccaacta ctgttagccc caatactgaa gcagcttcag ttaaataatt
   140641 aatcttttca ccgtattcag taacaatctt cattccctgc ttataaccaa attcaaagcc
   140701 tctcattcgg aaaaagaaga ttaccatgat taaaatgaac cataaaaaga ttcctacagg
   140761 attattttct aaagccatat aagccgcaat tgatcccatg atggttggga tcatcgtcat
   140821 caaaagagag tccccaatac cagcaattgg acccatcgta ccaactttaa agtcattaat
   140881 cgcatctttt gcttctaatc cttgcttttc ctccattgca acacaagcac ctaaaataat
   140941 ggctgccagc caaggctgtg tattgtaata ctgaaagtga ttatttaaag cttcgatgta
   141001 ttcatcatca tctttgtata attttcttaa aataggtgac aatgcgtaag tcacagatgc
   141061 accttgctgt gtttgataat taaaggtaca aactgacatc aaccaacgat agttcgcttt
   141121 cctaagatcc tttttcgtta cttgatattt ttcgctcatt ttattcatcc tccccaataa
   141181 acccaacatc agaagctccc gcagaagcag tgatttgtgc atctttttca gctctatgat
   141241 acacattaaa tgctaaagcg aatcctatga tcgccgctcc aataataggt accttcaaaa
   141301 aagcggctaa gaaaaatcca acaagtaaat aactaatgta tcgacctgta ggcataaagc
   141361 gtaacaacat cgcaatacca acagcaggca acatttttcc ggctaatgtt agcccatcca
   141421 taaaccaatt aggtaaaaca tccaaaacgt aattaacagc agactgtcct aaagtaacgg
   141481 caataaatac tggaattgct gcagataaac caaagaaaat tggccccaac cacaaaacac
   141541 tattcatttt cttaaattct cgtttattcg catattcctg tgatttgcga acaacaaaac
   141601 catttaagat tttgacaatt acatctagtt gcacccctaa catacctaca ggtagtccta
   141661 ttgctaatcc ggcttctact ccaagctggc taccaaatgc cgttgcaata attgcgcacg
   141721 ttccatagtc cggtacagat gagccaccaa aaccggcaac tcctaatgac atcaattgaa
   141781 ctgtaccgcc aactatcaaa cctgttttta tatcacccat caaaattcct gtgaataatc
   141841 cccaaaaaac tgcgtaattt acaaagatca tcggaatccc ataataatca acatgtttaa
   141901 taaatgctaa tatagtcagc ataataattt gaaaaatagt aaaatgcatt atccttactc
   141961 ctttatccta attttgattg aatttcctgt gccttatccg ccggaacaaa ttgtgtaaca
   142021 atttctacac cagaatccaa caatttttta tacgtgctga tattttcatc tgatgcatag
   142081 gctctagaag atagctgtaa tgcattatct ttaacaacag taccgccaac aattagtcgg
   142141 tcaattctaa taccgttatc tactaaatga aggattgttt ccggctcttt cacgatgaca
   142201 aatacttttt cctgatcgta tttccccttt ttaaaattcg caatcgctgt ttcttcagtg
   142261 ataattgata cagccattcc agcaggtcta gctaaacgca tacttgattt ttggatgtcg
   142321 ttatttgcga cagaatcatc gatcaccatc actctgtttg ctttagacat tggtgcccat
   142381 tgcgtgacaa taatcccatg caataaacgg ttatcaactc tcgacataac aatactcata
   142441 aatacttcct cctttatctt tcattttctt acaagcaatt accatgccaa aacaaaaaga
   142501 acttgataaa tcaagctctt tttacataat cataaacgta tttaacctca tgaatcggga
   142561 tgtcgacact ataataatgt tcaacgacac tgaaagattg cttaaaatgt gtctgaaatt
   142621 tttgaaattc ctcactcgtt tcattgaaat aaatttcatc ttccaaagta ttttgtttta
   142681 ctaatcgttc aattaagcaa ctaatgtgaa catataatcc gaaacacgta ttattatcaa
   142741 atgaaattcc tagatcattt tgtaagatgt atatagcatc tgaaacccgt tctaataatt
   142801 tagtcggatt taatatggtc aattgctcca ttaaattcgt tagactaaaa ttcttcagaa
   142861 tattattgtt caaaactttc agttcattat cattaaagtg tctattcaat tgattggaga
   142921 atttacttat attcattcct aaaatcatat cttcaattga aaagaattct acatcttcta
   142981 gatgtggatt tagtgttcca acaataaaaa gaatattgtt ctctttacga agctccgata
   143041 aatagttttc attcactaaa gagtaatagt cacacgtttc tatgacaaga tcagtatccc
   143101 ttggaaagct atcttcaagt atctttttta atttaaacga agttcctaat ccgcttgcac
   143161 aagaacagac aatcatatcc ttcttaatat ttgatgattc ataaatataa tccacatttt
   143221 gattttcttt tacttgttca agaatctgtt taagcggtaa atctaataat atctgatttg
   143281 caacatcaat agccattctt gttgaaacat tgtttagtag tccgaatttt ttttgagcgt
   143341 ctagttccaa atttagataa attttctcca gtgaacccat atcaacaagt aaaataattt
   143401 cttcatatcc ggtacactct tgtaagtatc tattaatttt gtctgcaatc tcactacttg
   143461 aagtcattag tggcatatca atggcgtcgt atatatatct ctcaacgatc ctattagcag
   143521 tacttgctat acaggttgcc gttagatctc catgagccaa aatcagtgct aaacgttttt
   143581 gctgaaattc tttactacac attaacttaa ataaaataac caaaacattt ttttgaattt
   143641 ttgttaaata aatatcccct atttgaataa aacgattgac taattgaact tctcgactac
   143701 accaactatt aatatcattg atgaaggaat ataatttatc gtccgatgtt aattggattt
   143761 gataaatcca agaattcaaa aagatagacg ttatttgaag ataataatag tcaaattcta
   143821 tttgaaaatc tctctctatt tccttaacat actgtctaaa tcgaatctct tgataggtgg
   143881 gatatgttaa agctgaatta ttttggatat caacattctt ttgtctaaaa tatctatcat
   143941 atttctcaaa aagctgctgc ttatcagtgt ctttcttcca cgtttgataa agatactcta
   144001 taagttcgtt gatattaaaa acttcgtcta ctttttccaa atcatccatt tgaatatatt
   144061 ttaattcatt ctgtcttttc ataaccttca tgccgttgaa attcacccat tgtggtaaat
   144121 gatacatctg aatataaagt tcttccaaat catctaagaa ataaaaggca ttggcacagg
   144181 tcaattgaat gatatttttc aaattgccaa cattttcact aaagcgatag tttaataaaa
   144241 aatctaataa ttgttgagat atcagtattt tctttgctag tctttcttct tctgatttaa
   144301 aaaatatctt gattaactgt tttctttcat aaagagaacg actgtctaat gttggaagtg
   144361 ttagctttac aggtattctt ctcaacaaag ttgataatag tttatcctca ggtggttctg
   144421 atgttgcaaa tataatccag caacttgatg tgtaccagtt ttcattatca cccatccgat
   144481 gaaatttcct cgtatccata aactgaaaaa gtttctcctg acattcattg ctcaatgcat
   144541 gaatttcatc taaaaataag actccattat tagctaattc tgctaaaccc tttcttccat
   144601 cttcggctcc tgtatatgca ccttttacat gcccaaaaaa attggacaag aaaagttctg
   144661 gattattggc atattcacta caattaactg aaatgaaaga tgccttctct tctatataat
   144721 tattggccaa caaatcctta tacacataat ttgctaaata gctttttcca gttccagtct
   144781 cgccatgaag taaaatgggt aagccatttg gcggatagta gattgctgct ttaatttgag
   144841 caattacatt tcgcagactt ccttgacttc cgataaccct ttctattcca accctaaaag
   144901 agctatcata ccatttttcc gcttcctctg aagagctaaa aatttgttga tttggaactt
   144961 tctccggtaa agaaaagaag attactggac gagtattgat ttttccaaat tctccatctt
   145021 ttacgaattc attcaaatat tgagagacaa tattacgact aacatttaat ttttcagata
   145081 ttttctgtgc tgtattgttc ccccatttat ttttaatctc agagttctct atgacataat
   145141 tgacaatgcg ttcttttaat tcttccatca cagcacctcc tcgacactat tcgaattttg
   145201 ccgacagtat ttttccataa atatgacttg ctaaaaaggc tttgtttaga tcattcaatt
   145261 ggaacgttgc ttgttttgct tgatccgcta agacgttgaa aagatactct ggcgaaatcg
   145321 cataccccaa aatatcaacg attgttgcaa tcagatcttc ttttgattct tcatctgaaa
   145381 gtaattcacg atacaacatc gtttgtttgc ttactgtgtc atattcctct aaggattcat
   145441 ctgctaaaag aaccacttgt tccaacaaac ggtcggataa atatttatag aaaatcagcc
   145501 ccaataaata atttttatat tctgacgcat ccattttact acggagatta tctgccgcac
   145561 taaataactt ctgatttaat tctgcaccca aaaagataca ctcctatctc ttatttaaag
   145621 ttgctatata ccctcttatt ttagcatact cagttttaga aaaagcaaaa aaataaagaa
   145681 acttattctt gataacaagc tacgtgacgt atatccctaa cacaaaaata gccatatcct
   145741 ttggtttgga tacggctatt tgatattact cattcaatta tcaaatcaca gtaggtgtga
   145801 atctcatcgg gatcggcaat atctacttct gattaggatc aaaggtgatt ttctcaataa
   145861 aggtgtgata caaagcttta acttgcttct tctcactctg gataagaaag caatccaaac
   145921 tagtcaatat ctgttggaca tctttgactt gaatcggttc cccttgttgt tgaaagatcc
   145981 ctaagatttc attctctcga atttgtaact gtcttcgttt ttcagtcaat tcatttaaac
   146041 gatcttttaa catcggaaac aattcgggac tatcttccaa agcagcttcc tgtttttgct
   146101 ctaatagtta gattttgcgt accattttct tcttttattt tccttcgatt gctgatttta
   146161 atatagtcta aaggaaattt atatttattg tattctttat aacttcaaga gaacttcgtt
   146221 tgagtattac tgtatttttt taattccata agaaaattag ttattacctg ccacctaatc
   146281 agcaaatctg aacctgagtc aatataatta atattatttt ccatctccaa cattgtaagt
   146341 actgttggtg ttaatatagg gactttgtac aattgtattt ttttgatttt atcatctgca
   146401 caaaggagat tttttaaatc taatccttct tttatttcaa aagttgaatt ttttaaatct
   146461 ataagagtaa aaccaattcc ttcatactga atttctgaaa atcgaatttt tctcccagtt
   146521 gatagatcaa ggggcagaaa accttttatt acattgtgag tgtcaccatc atttacaatg
   146581 tcattaggaa ttttgatttt agaattctgt ggagcaccta ttggaagata ataattcatt
   146641 ataattttgt agtcttcatg aggaaataaa gtacttttag gattgctaag atattctcgt
   146701 accgaatcta agaatggctt atccattgaa tcttcatccc catttaaact aaaaaacatt
   146761 cctacaattg cttttgcaac tctaagtgaa ttcattttct ttattgaatg tgttaaatca
   146821 gacttaccat tctttaaact tgtattgtta taaaggaaat ttttattgtc ttctgcaaat
   146881 ctaatatatt cctcaacata ctcactacca aaaaaactat tacatttact acataacg
//
LOCUS       DACDXO010000002       141372 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E23605_2017
            SAMN12639989-rid9881463.denovo.002, whole genome shotgun sequence.
ACCESSION   DACDXO010000002 DACDXO010000000
VERSION     DACDXO010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639989
            Sequence Read Archive: SRR10023389
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 141372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 141372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741914.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:42:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,972
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 33 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 17 of 73
            Pseudo Genes (multiple problems)  :: 10 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..141372
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639989-rid9881463.denovo.002"
                     /isolate="E23605_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            212..604
                     /locus_tag="IWM35_000133"
     CDS             212..604
                     /locus_tag="IWM35_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP9918020.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
     gene            632..1972
                     /gene="glnA"
                     /locus_tag="IWM35_000134"
     CDS             632..1972
                     /gene="glnA"
                     /locus_tag="IWM35_000134"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HAP9918021.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            3165..4775
                     /locus_tag="IWM35_000135"
     CDS             3165..4775
                     /locus_tag="IWM35_000135"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HAP9918022.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
     gene            5067..5936
                     /locus_tag="IWM35_000136"
     CDS             5067..5936
                     /locus_tag="IWM35_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HAP9918023.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            6101..7372
                     /locus_tag="IWM35_000137"
     CDS             6101..7372
                     /locus_tag="IWM35_000137"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HAP9918024.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            7369..8664
                     /locus_tag="IWM35_000138"
     CDS             7369..8664
                     /locus_tag="IWM35_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HAP9918025.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            8763..9026
                     /locus_tag="IWM35_000139"
     CDS             8763..9026
                     /locus_tag="IWM35_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HAP9918026.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            9471..9920
                     /locus_tag="IWM35_000140"
     CDS             9471..9920
                     /locus_tag="IWM35_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP9918027.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            9977..11395
                     /locus_tag="IWM35_000141"
     CDS             9977..11395
                     /locus_tag="IWM35_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9918028.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSTSTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            11395..12255
                     /locus_tag="IWM35_000142"
     CDS             11395..12255
                     /locus_tag="IWM35_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HAP9918029.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            12429..13202
                     /locus_tag="IWM35_000143"
     CDS             12429..13202
                     /locus_tag="IWM35_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP9918030.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            13349..13714
                     /locus_tag="IWM35_000144"
     CDS             13349..13714
                     /locus_tag="IWM35_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HAP9918031.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(13862..15070)
                     /locus_tag="IWM35_000145"
     CDS             complement(13862..15070)
                     /locus_tag="IWM35_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP9918032.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(15089..15367)
                     /locus_tag="IWM35_000146"
     CDS             complement(15089..15367)
                     /locus_tag="IWM35_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9918033.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            15560..16216
                     /locus_tag="IWM35_000147"
     CDS             15560..16216
                     /locus_tag="IWM35_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAP9918034.1"
                     /translation="MDSRVLKEYLDKNDFPIIRKGYHKYLTFEGMEDSHTYILKEGTV
                     KASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESEYAEFYRINRVRFW
                     QNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLYELYDLFGVRTEEG
                     MLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIVILNMDLLEDNIIF
                     "
     gene            complement(16658..17455)
                     /locus_tag="IWM35_000148"
     CDS             complement(16658..17455)
                     /locus_tag="IWM35_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HAP9918035.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(17452..18636)
                     /locus_tag="IWM35_000149"
     CDS             complement(17452..18636)
                     /locus_tag="IWM35_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HAP9918036.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(18661..19908)
                     /locus_tag="IWM35_000150"
     CDS             complement(18661..19908)
                     /locus_tag="IWM35_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virion core protein"
                     /protein_id="HAP9918037.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            20123..21868
                     /gene="dnaX"
                     /locus_tag="IWM35_000151"
     CDS             20123..21868
                     /gene="dnaX"
                     /locus_tag="IWM35_000151"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HAP9918038.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            21888..22202
                     /locus_tag="IWM35_000152"
     CDS             21888..22202
                     /locus_tag="IWM35_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HAP9918039.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            22227..22823
                     /gene="recR"
                     /locus_tag="IWM35_000153"
     CDS             22227..22823
                     /gene="recR"
                     /locus_tag="IWM35_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HAP9918040.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            22887..23531
                     /locus_tag="IWM35_000154"
     CDS             22887..23531
                     /locus_tag="IWM35_000154"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HAP9918041.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            23546..23875
                     /locus_tag="IWM35_000155"
     CDS             23546..23875
                     /locus_tag="IWM35_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9918042.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            23889..24827
                     /gene="holB"
                     /locus_tag="IWM35_000156"
     CDS             23889..24827
                     /gene="holB"
                     /locus_tag="IWM35_000156"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HAP9918043.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            24863..25687
                     /locus_tag="IWM35_000157"
     CDS             24863..25687
                     /locus_tag="IWM35_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HAP9918044.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            25680..26027
                     /locus_tag="IWM35_000158"
     CDS             25680..26027
                     /locus_tag="IWM35_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HAP9918045.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            26096..26968
                     /gene="rsmI"
                     /locus_tag="IWM35_000159"
     CDS             26096..26968
                     /gene="rsmI"
                     /locus_tag="IWM35_000159"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HAP9918046.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            27077..28198
                     /gene="dinB"
                     /locus_tag="IWM35_000160"
     CDS             27077..28198
                     /gene="dinB"
                     /locus_tag="IWM35_000160"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HAP9918047.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(28252..28854)
                     /gene="nrdG"
                     /locus_tag="IWM35_000161"
     CDS             complement(28252..28854)
                     /gene="nrdG"
                     /locus_tag="IWM35_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HAP9918048.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYAFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(29169..31358)
                     /gene="nrdD"
                     /locus_tag="IWM35_000162"
     CDS             complement(29169..31358)
                     /gene="nrdD"
                     /locus_tag="IWM35_000162"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HAP9918049.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGSFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
     gene            31684..32451
                     /locus_tag="IWM35_000163"
     CDS             31684..32451
                     /locus_tag="IWM35_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9918050.1"
                     /translation="MEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGEFVGIMGPSG
                     AGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQEFNLIDTLN
                     AKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQRIAAARALV
                     VQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTASYCDRILFIK
                     DGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            32441..34483
                     /locus_tag="IWM35_000164"
     CDS             32441..34483
                     /locus_tag="IWM35_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9918051.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
     gene            34764..34913
                     /locus_tag="IWM35_000165"
     CDS             34764..34913
                     /locus_tag="IWM35_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HAP9918052.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            34924..36438
                     /gene="dltA"
                     /locus_tag="IWM35_000166"
     CDS             34924..36438
                     /gene="dltA"
                     /locus_tag="IWM35_000166"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HAP9918053.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            36435..37640
                     /gene="dltB"
                     /locus_tag="IWM35_000167"
     CDS             36435..37640
                     /gene="dltB"
                     /locus_tag="IWM35_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HAP9918054.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            37685..37918
                     /gene="dltC"
                     /locus_tag="IWM35_000168"
     CDS             37685..37918
                     /gene="dltC"
                     /locus_tag="IWM35_000168"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HAP9918055.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWSTPNKIIQQATALKG"
     gene            37921..39189
                     /gene="dltD"
                     /locus_tag="IWM35_000169"
     CDS             37921..39189
                     /gene="dltD"
                     /locus_tag="IWM35_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HAP9918056.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            39330..40412
                     /locus_tag="IWM35_000170"
     CDS             39330..40412
                     /locus_tag="IWM35_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HAP9918057.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            40412..41215
                     /locus_tag="IWM35_000171"
     CDS             40412..41215
                     /locus_tag="IWM35_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HAP9918058.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            41285..42025
                     /locus_tag="IWM35_000172"
     CDS             41285..42025
                     /locus_tag="IWM35_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAP9918059.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            42036..43040
                     /locus_tag="IWM35_000173"
     CDS             42036..43040
                     /locus_tag="IWM35_000173"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HAP9918060.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
     gene            complement(43085..44929)
                     /locus_tag="IWM35_000174"
     CDS             complement(43085..44929)
                     /locus_tag="IWM35_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9918061.1"
                     /translation="MSFFNNIYNKKESFYLRIKWSIIIILSLLFLTGIAYFFYFIFSP
                     ADSNQMKEIKDSWVFYTKDDPDFKFDSKYTRTIPTVDKDETFIMETTLEKPLDEANLL
                     IKGNHQWITVYLGEKILYQRKDYQEESNPGLSLAVIDLPANYIGKKLTLTVSSPYQNY
                     AGLPPKVYLGTTGPLISFIYSQSVPQVLTMIIAVFIAIGTFFYTIYLMYKQKRLEYSL
                     ILLSCFALALGFEAVSEDILSGILFEPFVHSFLSHLFIILTSAFIICYYWSRMFYYKK
                     WYGIFCTFQLLIFIGVLLYATFTPAELPELMPIANIASVISTLVTSLACIGEAYKNNR
                     FYVACTPWIVLVAIIHCLIYIQTALGIYQTTINWSTILYIIILIVIISYNLIDHILNT
                     DKDRRQVDFLKIKNDLLEQHYEQLRQHIQEVNTLRLEFVQEMENLQDLMHTDQTKAKN
                     YVEKILEEAKNFEMLCSFCNHQVTNLILARYQKLAAKRNIQITFQADLPEELPINDDD
                     LAQLLIHALEHSFRETHAIENPLYRKIYLSIHEQEEHFVICCEHSAHYDTNLFSRGIT
                     EELDDQERFDLMMMKDVADRYTGTLTQEKDTFIDRITVQLSRNYPNPV"
     gene            45141..45659
                     /locus_tag="IWM35_000175"
     CDS             45141..45659
                     /locus_tag="IWM35_000175"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAP9918062.1"
                     /translation="MPDYKKITFISSITLIIFLILILLVVLVPRIFGLTPYIIEDKEM
                     SPYYAQGNLIYVRPKNTNEILVGDIITYYENSGKKISTRRVIAVENNHEDFYTKADTK
                     TYIEPGVVKKRNIIGSPIFQIPYIGLVLSKTAFAWIQGLFFTIAFCLTGITAWNTFVE
                     LKKKRRRTQKFL"
     gene            45787..46470
                     /locus_tag="IWM35_000176"
     CDS             45787..46470
                     /locus_tag="IWM35_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HAP9918063.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            46779..46852
                     /locus_tag="IWM35_000177"
     tRNA            46779..46852
                     /locus_tag="IWM35_000177"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:46813..46815,aa:Arg,seq:tct)
     gene            47011..47370
                     /locus_tag="IWM35_000178"
     CDS             47011..47370
                     /locus_tag="IWM35_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 family protein"
                     /protein_id="HAP9918064.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLALLALLSIFL"
     gene            47493..49124
                     /locus_tag="IWM35_000179"
     CDS             47493..49124
                     /locus_tag="IWM35_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HAP9918065.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQIPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
     gene            complement(49186..49827)
                     /locus_tag="IWM35_000180"
     CDS             complement(49186..49827)
                     /locus_tag="IWM35_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HAP9918066.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     gene            50109..50417
                     /gene="rplU"
                     /locus_tag="IWM35_000181"
     CDS             50109..50417
                     /gene="rplU"
                     /locus_tag="IWM35_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HAP9918067.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     gene            50444..50788
                     /locus_tag="IWM35_000182"
     CDS             50444..50788
                     /locus_tag="IWM35_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HAP9918068.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            50801..51094
                     /gene="rpmA"
                     /locus_tag="IWM35_000183"
     CDS             50801..51094
                     /gene="rpmA"
                     /locus_tag="IWM35_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HAP9918069.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            51501..54335
                     /locus_tag="IWM35_000184"
     CDS             51501..54335
                     /locus_tag="IWM35_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP9918070.1"
                     /translation="MAKRIDRIYMYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNS
                     SKDLNQLVREGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDT
                     KDIFAKIIGANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAH
                     HVIDEETELIVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLD
                     EIHRLPPEGQEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRI
                     PINIKLPAFEKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQL
                     KSNIQLVCARGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKIT
                     VAPNDTIMKIQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFY
                     KDHGFSFNADSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKK
                     INLGEERHTNDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLR
                     ANQASGRIGVVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNV
                     QEVDEGSGVILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDL
                     DMLHESLKNFRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNK
                     RQETELEVLALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVI
                     LDQLLMESELEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTT
                     GIGDEEYGFKINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLED
                     QIKVKVPIEEMYYLLRLVHNQLDKKEYTVP"
     gene            54526..55017
                     /locus_tag="IWM35_000185"
     CDS             54526..55017
                     /locus_tag="IWM35_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIB"
                     /protein_id="HAP9918071.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            55120..56109
                     /gene="manX"
                     /locus_tag="IWM35_000186"
     CDS             55120..56109
                     /gene="manX"
                     /locus_tag="IWM35_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HAP9918072.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            56142..56945
                     /locus_tag="IWM35_000187"
     CDS             56142..56945
                     /locus_tag="IWM35_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HAP9918073.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            56966..57880
                     /locus_tag="IWM35_000188"
     CDS             56966..57880
                     /locus_tag="IWM35_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HAP9918074.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            57963..58337
                     /locus_tag="IWM35_000189"
     CDS             57963..58337
                     /locus_tag="IWM35_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HAP9918075.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGDNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            58476..58721
                     /locus_tag="IWM35_000190"
     CDS             58476..58721
                     /locus_tag="IWM35_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAP9918076.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            58893..59957
                     /locus_tag="IWM35_000191"
     CDS             58893..59957
                     /locus_tag="IWM35_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HAP9918077.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            60203..60628
                     /locus_tag="IWM35_000192"
     CDS             60203..60628
                     /locus_tag="IWM35_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAP9918078.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            60621..61070
                     /gene="nusB"
                     /locus_tag="IWM35_000193"
     CDS             60621..61070
                     /gene="nusB"
                     /locus_tag="IWM35_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HAP9918079.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            61167..62021
                     /locus_tag="IWM35_000194"
     CDS             61167..62021
                     /locus_tag="IWM35_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HAP9918080.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            62022..63359
                     /locus_tag="IWM35_000195"
     CDS             62022..63359
                     /locus_tag="IWM35_000195"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HAP9918081.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            63364..63588
                     /locus_tag="IWM35_000196"
     CDS             63364..63588
                     /locus_tag="IWM35_000196"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HAP9918082.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            63590..64480
                     /locus_tag="IWM35_000197"
     CDS             63590..64480
                     /locus_tag="IWM35_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HAP9918083.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            64489..65307
                     /locus_tag="IWM35_000198"
     CDS             64489..65307
                     /locus_tag="IWM35_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HAP9918084.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            65414..65863
                     /locus_tag="IWM35_000199"
     CDS             65414..65863
                     /locus_tag="IWM35_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HAP9918085.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            65881..67563
                     /gene="recN"
                     /locus_tag="IWM35_000200"
     CDS             65881..67563
                     /gene="recN"
                     /locus_tag="IWM35_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HAP9918086.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(67618..67734)
                     /locus_tag="IWM35_000201"
     CDS             complement(67618..67734)
                     /locus_tag="IWM35_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HAP9918087.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(67807..68763)
                     /locus_tag="IWM35_000202"
     CDS             complement(67807..68763)
                     /locus_tag="IWM35_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAP9918088.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            68934..69302
                     /locus_tag="IWM35_000203"
     CDS             68934..69302
                     /locus_tag="IWM35_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HAP9918089.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            69472..69903
                     /gene="mraZ"
                     /locus_tag="IWM35_000204"
     CDS             69472..69903
                     /gene="mraZ"
                     /locus_tag="IWM35_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HAP9918090.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            69924..70883
                     /gene="rsmH"
                     /locus_tag="IWM35_000205"
     CDS             69924..70883
                     /gene="rsmH"
                     /locus_tag="IWM35_000205"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HAP9918091.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            70888..71289
                     /gene="ftsL"
                     /locus_tag="IWM35_000206"
     CDS             70888..71289
                     /gene="ftsL"
                     /locus_tag="IWM35_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HAP9918092.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            71290..73482
                     /locus_tag="IWM35_000207"
     CDS             71290..73482
                     /locus_tag="IWM35_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HAP9918093.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGSAYGVYSVPGYNVSAKNGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            73508..74470
                     /locus_tag="IWM35_000208"
     CDS             73508..74470
                     /locus_tag="IWM35_000208"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HAP9918094.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            74484..75845
                     /locus_tag="IWM35_000209"
     CDS             74484..75845
                     /locus_tag="IWM35_000209"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HAP9918095.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            75871..76959
                     /gene="murG"
                     /locus_tag="IWM35_000210"
     CDS             75871..76959
                     /gene="murG"
                     /locus_tag="IWM35_000210"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HAP9918096.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            76974..78194
                     /locus_tag="IWM35_000211"
     CDS             76974..78194
                     /locus_tag="IWM35_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HAP9918097.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            78350..79675
                     /gene="ftsA"
                     /locus_tag="IWM35_000212"
     CDS             78350..79675
                     /gene="ftsA"
                     /locus_tag="IWM35_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HAP9918098.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            79698..80939
                     /gene="ftsZ"
                     /locus_tag="IWM35_000213"
     CDS             79698..80939
                     /gene="ftsZ"
                     /locus_tag="IWM35_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HAP9918099.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            80956..81633
                     /locus_tag="IWM35_000214"
     CDS             80956..81633
                     /locus_tag="IWM35_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HAP9918100.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            81647..82246
                     /locus_tag="IWM35_000215"
     CDS             81647..82246
                     /locus_tag="IWM35_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HAP9918101.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            82261..82548
                     /locus_tag="IWM35_000216"
     CDS             82261..82548
                     /locus_tag="IWM35_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HAP9918102.1"
                     /translation="MYTIALLLHLLQRAAYFYSIILMVYALLSWFPGGYQSGLGRLLA
                     KICEPYISLFDRLPLRIGPLDFTILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            82597..83379
                     /locus_tag="IWM35_000217"
     CDS             82597..83379
                     /locus_tag="IWM35_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HAP9918103.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            83400..84107
                     /locus_tag="IWM35_000218"
     CDS             83400..84107
                     /locus_tag="IWM35_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HAP9918104.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            84369..87167
                     /gene="ileS"
                     /locus_tag="IWM35_000219"
     CDS             84369..87167
                     /gene="ileS"
                     /locus_tag="IWM35_000219"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HAP9918105.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKGYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(87216..88742)
                     /locus_tag="IWM35_000220"
     CDS             complement(87216..88742)
                     /locus_tag="IWM35_000220"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HAP9918106.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(88757..89404)
                     /locus_tag="IWM35_000221"
     CDS             complement(88757..89404)
                     /locus_tag="IWM35_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HAP9918107.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            complement(89588..89917)
                     /locus_tag="IWM35_000222"
     CDS             complement(89588..89917)
                     /locus_tag="IWM35_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HAP9918108.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            90094..90822
                     /gene="sfsA"
                     /locus_tag="IWM35_000223"
     CDS             90094..90822
                     /gene="sfsA"
                     /locus_tag="IWM35_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HAP9918109.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            90838..91851
                     /locus_tag="IWM35_000224"
     CDS             90838..91851
                     /locus_tag="IWM35_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP9918110.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            91851..93128
                     /locus_tag="IWM35_000225"
     CDS             91851..93128
                     /locus_tag="IWM35_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAP9918111.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            93191..95893
                     /locus_tag="IWM35_000226"
     CDS             93191..95893
                     /locus_tag="IWM35_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP9918112.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYAQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            96045..96362
                     /locus_tag="IWM35_000227"
     CDS             96045..96362
                     /locus_tag="IWM35_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP9918113.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            96392..96712
                     /locus_tag="IWM35_000228"
     CDS             96392..96712
                     /locus_tag="IWM35_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAP9918114.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            96820..98280
                     /locus_tag="IWM35_000229"
     CDS             96820..98280
                     /locus_tag="IWM35_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP9918115.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGTLPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGPNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            98348..98569
                     /locus_tag="IWM35_000230"
     CDS             98348..98569
                     /locus_tag="IWM35_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9918116.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            98600..98782
                     /locus_tag="IWM35_000231"
     CDS             98600..98782
                     /locus_tag="IWM35_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HAP9918117.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            98782..99195
                     /locus_tag="IWM35_000232"
     CDS             98782..99195
                     /locus_tag="IWM35_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HAP9918118.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            99318..100499
                     /locus_tag="IWM35_000233"
     CDS             99318..100499
                     /locus_tag="IWM35_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9918119.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            100888..100961
                     /locus_tag="IWM35_000234"
     tRNA            100888..100961
                     /locus_tag="IWM35_000234"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:100922..100924,aa:Arg,seq:cct)
     gene            complement(100998..101198)
                     /locus_tag="IWM35_000235"
     CDS             complement(100998..101198)
                     /locus_tag="IWM35_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP9918120.1"
                     /translation="MKINYTVDLVSFSLVINFSLFSLRLFIDGLRHTHASILLYQGVN
                     ILSVSKRLGHSSLETTMSTYTK"
     gene            complement(101503..102735)
                     /locus_tag="IWM35_000236"
     CDS             complement(101503..102735)
                     /locus_tag="IWM35_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HAP9918121.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
     gene            102989..103558
                     /locus_tag="IWM35_000237"
     CDS             102989..103558
                     /locus_tag="IWM35_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP9918122.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(103736..104176)
                     /locus_tag="IWM35_000238"
     CDS             complement(103736..104176)
                     /locus_tag="IWM35_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HAP9918123.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            104334..105098
                     /gene="map"
                     /locus_tag="IWM35_000239"
     CDS             104334..105098
                     /gene="map"
                     /locus_tag="IWM35_000239"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HAP9918124.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            105130..106053
                     /locus_tag="IWM35_000240"
     CDS             105130..106053
                     /locus_tag="IWM35_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HAP9918125.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            106129..107268
                     /locus_tag="IWM35_000241"
     CDS             106129..107268
                     /locus_tag="IWM35_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HAP9918126.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIVLIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            107261..108061
                     /locus_tag="IWM35_000242"
     CDS             107261..108061
                     /locus_tag="IWM35_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP9918127.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            108061..108888
                     /locus_tag="IWM35_000243"
     CDS             108061..108888
                     /locus_tag="IWM35_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP9918128.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            108866..109600
                     /locus_tag="IWM35_000244"
     CDS             108866..109600
                     /locus_tag="IWM35_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAP9918129.1"
                     /translation="MLRIGRCEFVRSVCIATFNGGRYLREQLDSILSQLSAEDEVVIS
                     DDGSVDDTWDILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWL
                     ETKVKKITDYFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYI
                     GAGMAFRSSLKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIET
                     HSKLRQKISWRVHLITALIKRKWFKR"
     gene            109700..110566
                     /gene="rfbA"
                     /locus_tag="IWM35_000245"
     CDS             109700..110566
                     /gene="rfbA"
                     /locus_tag="IWM35_000245"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HAP9918130.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            110580..111152
                     /gene="rfbC"
                     /locus_tag="IWM35_000246"
     CDS             110580..111152
                     /gene="rfbC"
                     /locus_tag="IWM35_000246"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HAP9918131.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            111174..112202
                     /gene="rfbB"
                     /locus_tag="IWM35_000247"
     CDS             111174..112202
                     /gene="rfbB"
                     /locus_tag="IWM35_000247"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HAP9918132.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTSILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWQPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            112300..113151
                     /gene="rfbD"
                     /locus_tag="IWM35_000248"
     CDS             112300..113151
                     /gene="rfbD"
                     /locus_tag="IWM35_000248"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HAP9918133.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLPYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEALGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            113186..115219
                     /locus_tag="IWM35_000249"
     CDS             113186..115219
                     /locus_tag="IWM35_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9918134.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFSLV
                     NEDYSLNGQNMIIAYNSPVKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMIAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFVALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWEKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            115263..116543
                     /locus_tag="IWM35_000250"
     CDS             115263..116543
                     /locus_tag="IWM35_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HAP9918135.1"
                     /translation="MKIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGVGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LVILFCLVIKWIEWTKWDRGIYLIVFTVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNTIGSSLMTLALLISGFASSFERWYVRIIGGIAVVAAFLGIWVCQSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTDREDIWHKFY
                     QTLGEKSEQVLLGMKTFIFQRGNQFLGNHNSYNSILNIYGLIGFGIAALLLILFIGRL
                     TLKADLSNGQMTFIWAFFAVMMQSFMEDTLTSYPWVPIIYILLAMAVHRYDEPEVAEE
                     FYVMEEAYDDMTYDDTSYEEQPDSAHSSRIQRYH"
     gene            116753..117559
                     /locus_tag="IWM35_000251"
     CDS             116753..117559
                     /locus_tag="IWM35_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9918136.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            117571..118791
                     /locus_tag="IWM35_000252"
     CDS             117571..118791
                     /locus_tag="IWM35_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9918137.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGEVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     DKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            118781..120367
                     /locus_tag="IWM35_000253"
     CDS             118781..120367
                     /locus_tag="IWM35_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP9918138.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLFINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVAQSSIAEPQNSNYVKVLEVTQQTKQDETIQQIPFNNFTGENETLSFPVSETT
                     ERMVFRFAQQPSFIEFSAEAAGEKLTFIDSNAVVKTVNDCYLFDGKELPEFVLTDISG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENHHLLEENTRLD
                     HTLKTTTNRYCTLLESDEWTIKHRLGRRKKETSKKIQEKELSICIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVSREEVNQAEQLAKGTEAGFELRILCEQ
                     ERSFLVEAVAQNGQSWIIEM"
     gene            120406..122544
                     /locus_tag="IWM35_000254"
     CDS             120406..122544
                     /locus_tag="IWM35_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAP9918139.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKVRLSGKMRGKAILDFQTARHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKAIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEAQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRKENGHISLATNSALEIAEGEFIALLDNDDELPPF
                     ALYEVAKVLNVHPELDVIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKELGGFRKGYEGSQDYDLVLRVTEQIPADHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVRPGRISGFYEIAYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYSNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTVMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     TLEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPAYIAHDPFYNDNLTKDGIGDFSLRPD"
     gene            complement(122912..123895)
                     /locus_tag="IWM35_000255"
     CDS             complement(122912..123895)
                     /locus_tag="IWM35_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HAP9918140.1"
                     /translation="MNKQELHDFIGFRQPNICQIVAYKDGVEVYTDEWNGYKKDDTCH
                     IMSATKSIVSLLIGIALEQGLIKSIDDKVLDYFPEYKVKRGEKTIYAITIKHLLTMKA
                     PYKCKGDPWTKVCSSEDWTKTSLDLLGGRKGITGEFNYQTVCLHILTGLLYQTSKLTT
                     VDFANKYLFDPIGIPNHVNYYAETAEEHKQFTMDKSPKTNVWFCDPLNIGTAGYGLCL
                     SASDLAKIGVLCLNKGMYKGKQIVPPMWINEMFKPRTSALEDLQNMRYGYLWWVLDSE
                     NEIYSAIGNSGNVLYIDAKNNLVISITSYFKPMIIDRVAFVEEYLKPFILK"
     gene            124273..125664
                     /locus_tag="IWM35_000256"
     CDS             124273..125664
                     /locus_tag="IWM35_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HAP9918141.1"
                     /translation="MNKNGEWSAARRIFIILMDVIVYNAAIYSSFLLKFRGEIPLRNL
                     ETFQHSAIIISVIFIALNILLGAYVFYNRMISDIVFVTVIGQVLMTLGIMVVTFAGRW
                     FAFPRLVILLSFVLGTLFLSIYRILVYKLYLRVSRDKKVVIIGLEKDVAPAIQNFGSK
                     KNNKHKVEAVILDNYYANIKNMIKDIDIVYLASHIEETEKIKIYQLVTKNEKKLFLNT
                     TFENLTLINPNIMNFEDESIIEASGFRIQPEYELFKRLFDIIVSLVLLVIASPFMLFT
                     AILVKVTSPGPIIYKQVRITKNQKEFSIYKFRSMTATAEAKSGPVLAKSNDARVTPVG
                     KFIRAVRFDELPQIFNVLKGDMSIVGPRPERSFFVDQFNAENPYYYLRHNVRAGITGY
                     AQVYGKYVSDYNSKLRFDLLYIKKYSLMLDLKILLQTIKILFDKMSSQGLEEDENVIS
                     LAEIAFPNEKIFR"
     gene            125680..126720
                     /locus_tag="IWM35_000257"
     CDS             125680..126720
                     /locus_tag="IWM35_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAP9918142.1"
                     /translation="MNNPKFSIIVPVYNAEATIGRTIDILKELDYEEYEVVLVNDGST
                     DQTQEIIQELIENDERFELITTTNQGPGLARNEGIRQSKGEYLLFFDADDTPVKTILS
                     DYDHLLEKSPKADLVVSSFVFRTMDEGRIVSEKENLVTECHYLTHEEFIKDMYQLMNE
                     QLMYVVWNKCYRSDILKNNQILFKTYNSCEDRIFNLHYYEYCHQVIMNPKVEYIYEFE
                     GGKGITNQYRPNKFETFKEFYSLANTVTDDINKDGMAALLLKGTTSVIFSIYSTSART
                     SKEKKDEAKQILSDPVIREAKQIAKTDSSAKKITKLLYNMPAPLFLSAVKVGSFVEVK
                     MPGLMAALKRVY"
     gene            126739..127824
                     /locus_tag="IWM35_000258"
     CDS             126739..127824
                     /locus_tag="IWM35_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HAP9918143.1"
                     /translation="MKRILHFQGRMGLGGAESFMMNLYRKIDRTNYQFDFLIYEDYAD
                     VQDYHSEIERLGGRIFVVPNPKKNIFKYLIEVNKLLKKESFSIVHNQVYFGGGINLWL
                     AKKNGIRQRIAHSHATEDGKSQNIVMNVLRKFLTKLLLQNATDYLAVSQEAGESLFQN
                     HPFEIVHNGIDLELYSKNSEAKVNKRKELDISMSTFVVGNIGRLEKQKNQSYLLEIFL
                     EILKMNSDSYLILVGEGSLRNKLKAKAVELDIEKRVKFLGERNDIPELLSVMDVFVMP
                     SLYEGLPMVGLEAQAAKLKLVLADTISKDTKLTNNVDFVSLDETPQEWAKKVIKEPYA
                     NKITQELKSYDVSYTTEQMESIYSRDH"
     gene            127857..128819
                     /locus_tag="IWM35_000259"
     CDS             127857..128819
                     /locus_tag="IWM35_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-1,6-N-acetylglucosaminyltransferase"
                     /protein_id="HAP9918144.1"
                     /translation="MKICYLILAYNDIGNLKRLVNRLNENAVFIIHIDKKTDIEPFIE
                     AFKSYDNVEFLCNKNRVKIYWGGFSIVQAEINLVKRALQNEKYLKYVLLSGADYPIKD
                     NEYIYNYFKKNSSVEFIRGIDLDQIKHKEFYYKHIDVYQKHDYPRINRNNTTAFKIFR
                     AIINRCLRMIKLPPKIRHHKFDLYHGSQWWALSKECLTELIQMYEQNQQDYLNFKIGM
                     FAPDEKFFHTLFFNSSFKNKNVIGGPDLPLELKNIEETTLQTSKLANIHIIDPSMNKW
                     FDERDIDQIKESEKLFVRKVKSDYSDKLLNRIDKEILKVGNIDE"
     gene            128812..130239
                     /locus_tag="IWM35_000260"
     CDS             128812..130239
                     /locus_tag="IWM35_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HAP9918145.1"
                     /translation="MNRIVPVLEKSLLSRDAFRVINLIICLLYFMPSTFNVTNIPMKI
                     AFAWGGIIVLYEVFIKKNFFKMRFSWLLIGAMISFILTIIINRENFLIPSIYNFGYLL
                     ITLIVIFPADFSQSEEEKKKKMVYFNNVFIIIIFLAALISIYQFIFLISYHVPTGTPG
                     LLARQGFIEHRLFGVYTSPNVGAMFGYTSIILSLISVLISKITKTIRVFYMMNFIVQI
                     FYITLSSSRGAQVVICSFLVLFLLLFANSKFRKSIREVLPVKIKWMVSILLVLLVYFS
                     FGTNYAKDFLATIPVSIDRKVEVIESNESDKTPLPEKEVILQHSSDNSEISAGRFTIW
                     KAALKVIRPTLLFGYGETDFYGNSDAKFLKKVDLDTTDVNELKRAHGNMHNGFLQVLV
                     SSGIIAFICIYAFYVLNIFSLLKTFFRSKVNYAFLGIVLIFILSIFIDEMVEAHVLFN
                     KRDVISIVFWYYLGSISYLYLKKEN"
     gene            130241..131311
                     /locus_tag="IWM35_000261"
     CDS             130241..131311
                     /locus_tag="IWM35_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9918146.1"
                     /translation="MKTAFIAWTPLHLINILNVYQNFYRESKGDLFIYSEFENARRYS
                     ERIKELGLFNDVVFVNHRKIGSSFKRKINLLTNHNQFFSDDLSVYDEIFIQGENYFTK
                     ILFSNVYKKNPDVKLNYIEDGLGTYLKKSIFSTSKNVQRVFKILNKNSIFKKKIDDYF
                     VYCPELLLYDVKNIKKIPKIDENSKLMKNIQSIFGIEEVFVGDKCIFFDQPLLSDGYG
                     IDETDIFAKVNSIFSGKKETLIKLHPRARTNRYGEKNILNSSLPFEVLLLENDFSSTL
                     LISPISTISFSPSMMFEKTINSIMLAKLILNEYTALSEDKKETLTNIVLFCEHYNRLS
                     EEKILLPENWAELRKLVEYGII"
     gene            131298..132719
                     /locus_tag="IWM35_000262"
     CDS             131298..132719
                     /locus_tag="IWM35_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HAP9918147.1"
                     /translation="MGLFNTLSKSKLVKSSFWYTIGNFFIKGISFITIPIFVNITTQA
                     EYGLINNFTSFVSIFSILVGLSLNSAINNASFDYKDDINEFMSSVLSLATLSLIVFLL
                     IGNIYFLFSNAFFDMNQLVFNLMLLQSFASFVISYIQSYFTINFNHFKYLILSFISTF
                     LNIAISVALMQTLYFDDKYVGRVVGGTLSFVLIGLVIYLRIMIKGAHFFDKKQWRYAL
                     KISLPLIPHSLANIVLAQFDRIMINSYLGPAAGGIYSYISNIGIILSVIWMSTNNAWV
                     PWFYGEMSKNNEKKVKKVSNYYLVVFTLITMIVMVVSIDLAKFMAPKDYYSGLSLVVP
                     ITLGYFFQFLYSLPVNAEFYEKKTSFIAIGTVASAIVNILLNMIFIPRLGIIAAGYTT
                     VFTYFLLFIFHYNIAKKITNKQLFDTKKICIIIIFVSIFSLILHILINQMLIRYVLTS
                     LLIISLVIYFLKIKNRIIDDYNS"
     gene            132732..133739
                     /locus_tag="IWM35_000263"
     CDS             132732..133739
                     /locus_tag="IWM35_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAP9918148.1"
                     /translation="MFKGKTILVTGGTGSIGSEIVRQLLKYEPKAIRIYSRGEEKQFY
                     MQQELSNHTNLRFLIGDIRDSQRIDYACRDVDIIFHAAAMKHVPASEYNPMEAVKTNV
                     MGTQNVIDAAIKNNVKHFVGISTDKVVSPTNTMGATKLLSEKLIIAANQYKGKTETIF
                     SCVRFGNVMGSSGSVIPLFSKQIKNNKDITLTDVDMTRFMMSIPDAARLVLSAAMNSK
                     FGETYVLKMPAINILTLAQSLIKIYNDTHDEKYRGEIKVVGIRPGEKLYEELMTTDEI
                     ERSYENDKMYIIPPHYHATDELYKDYEKIKITAYSSDATELMSGQEFIRIAKENNVVT
                     W"
     gene            133751..134755
                     /gene="neuB"
                     /locus_tag="IWM35_000264"
     CDS             133751..134755
                     /gene="neuB"
                     /locus_tag="IWM35_000264"
                     /EC_number="2.5.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase"
                     /protein_id="HAP9918149.1"
                     /translation="MKSEVYVIAEIGVNHNGSLDLALESIDKAKECGANAVKFQTFKT
                     NKLVSKKAPMAKYQKDNLKEDSSQFEMLKKLELSQSDFIRIKDYCIQNKIDFLSTPFD
                     EESADFLKQIGVQGFKIGSGDLTNLPFLRKIDMYGLPVLLSTGMADIHEVSEAVDCFI
                     DSPVTILHCTSNYPASSEDINLLAINTLKEMFGIPVGYSDHSLGYDVGICAVAMGAKV
                     IEKHFTLDKELPGPDHKASLDPQEFADFVKHIRNTEIFLGDGVKRAMPSELSTKEVSR
                     KSLVVTENLNVGDILTEKSLTIKRPGNGIAPKFLYQYIGKKVKRPISADSVLLEEDVE
                     "
     gene            134752..135900
                     /gene="neuC"
                     /locus_tag="IWM35_000265"
     CDS             134752..135900
                     /gene="neuC"
                     /locus_tag="IWM35_000265"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (hydrolyzing)"
                     /protein_id="HAP9918150.1"
                     /translation="MKKILIVSGTRADYGIYKPVAENLLQDNWDVSFVVSGMHLSHKY
                     GFTKELILADGFRISGEVQSLFLENNKGNMARSVALEINGFTNIFEQTSPDFVLLLGD
                     RGEMLAAAIACLYLGIKTAHIHGGEVSGTVDESIRHAITKIASVHFAATEKSKERLIK
                     MGEDSWRVFPVGAPRIDTILNNRLPSFRDIKEKYHLKFEKKKYVLQVFHPVVTELENI
                     EKQILALINSMEQQSQPIICILPNSDAGSEIIRKMYQKYLKTDIIYITNLEPEEYLTI
                     LKECKFMIGNSSSGIIEAASFKIPVINLGTRQNGREQSKNIINANLNENEIETALNKI
                     QDPQFIKSLKNVKNIYGDGNSSRRITKTLNQILNDGEDIKWIQKQIAY"
     gene            135873..136550
                     /locus_tag="IWM35_000266"
     CDS             135873..136550
                     /locus_tag="IWM35_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HAP9918151.1"
                     /translation="MDSKTNSLLKYTSDPYIIIGAGGHAKVIIETLRLENKLIYGFFD
                     DKQNKMQDLKYLGKINELNSWADSKKKIYKFIVAIGDCVVRKKIIKKLNMPSEQYGIA
                     VHPSAIVAKEISIGYGTVIFANAVINPDAVIGEHAIINTGSIVEHDCRINNYVHLSPG
                     VCLAGGVHVGVGTQIGIGSQCIQMKKIGSWCMIGAGSTIVKDIPSHSLAYGNPAKIKK
                     EGINFEF"
     gene            136540..137682
                     /locus_tag="IWM35_000267"
     CDS             136540..137682
                     /locus_tag="IWM35_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HAP9918152.1"
                     /translation="MNSRILLSSPHMGGTEEKYVAKAFKDNWIAPLGENVDKFEESIT
                     KYTGSKGGASALSSGTAAIHLALRLLNVGKNDIVFAPSFTFIATVNPILYQGAVPVFI
                     DSEPNTWNMSPVALESALEEAERNNQLPKAIIVVNLYGQMADYERILAIANQFNVPVI
                     EDAAESLGSTYKGISSGLFGTIGIYSFNGNKIITTSGGGALVSSDKKYVEHSRFLATQ
                     ARDQAPYYQHSEIGYNYRLSNISAGIGRGQMEVLNDRVAKRREIFNRYKEAFSGIDRI
                     EMMPEVDGMVPNRWLSTLTINDYNSIDQVVNVINKLNEKNIETRALWKPLHLQPVFQG
                     VKMYYHDNNLSVSEKLFNSGLCLPSGSNMTDSEQEFVIDEFKKIFK"
     gene            137695..138672
                     /locus_tag="IWM35_000268"
     CDS             137695..138672
                     /locus_tag="IWM35_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP9918153.1"
                     /translation="MNIGIIGLGSMGKRRLRLIQEHFNDILLCGVDTNEARRKEVEKE
                     FGIPTYNSLTEAKNLFEINSVFVCTSPITHEFIIKEALNLDCNIFTEINLLNEYYDEI
                     IEVAKKKDRLLYLSSTFLKRKEIQYIKSKVTHNKKISYQYHVGQYLPDWHPWEDYRNF
                     FVSNKKTNGCREIFAIELPWIIDTFGKVKSFTVMTQKLSNLDIDYSDSFTLIIEHETG
                     IVGTLSVDVVSRVAKRNLTIIGEKILIEWEGNPDSLFSWNEETSSMTDIQLYDEVMHD
                     NNYSHTIIEDAYLEEIKEFFTLLEKKMEPQYTFEKDKYIINLINDIEVN"
     gene            138672..139580
                     /locus_tag="IWM35_000269"
     CDS             138672..139580
                     /locus_tag="IWM35_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HAP9918154.1"
                     /translation="MMKVIFFGLGSIGKRHLKNLISYGKKNSINFDITAYSSSGKKEF
                     EEITYIQNRDDLDTHYDIAFITNPTFKHLETLNVIVEKVDYIFLEKPAFSESIDLTQY
                     NYDYKNIYVAAPLRYKNIMEVVRNSIQSKKVYSVRAICSTYLPDWRKGTDYRNNYSAF
                     KSMGGGVELDCIHELDYIINLFGFPKSVKKQFGKYSNLEINSNDLATYILRYDDKIIE
                     IHLDYFGRVPTRKFQLFTLDECINVDLLNNKISFEENIVKEMPESANDMYEKELKYFF
                     ENVVTGKENWNDLYHANEVLKIAEEK"
     gene            139581..140333
                     /locus_tag="IWM35_000270"
     CDS             139581..140333
                     /locus_tag="IWM35_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HAP9918155.1"
                     /translation="MEILFTICGRAGSKGFKNKNLKYLAGKPLVHFALAMIDIYQQKH
                     PEHNCTTCLNTDSNELVELTTKLNPSVKIVERKPELATDTVGKIEVIRDSFLQLERED
                     NIFDLIIDLDLTSPLREVSNLEELIDEHLKNLDKDVYFSVVSARRNPYFNMVKRIENE
                     VTIVNKSRYTSRQQTPEVFEMNASMYSFKPEFLKKSNYIFDGQCGIIKMRDYLILDID
                     SEEDFKWLEFLFPKFLEDSSELQDIYNYLYRR"
     gene            140338..141285
                     /locus_tag="IWM35_000271"
     CDS             140338..141285
                     /locus_tag="IWM35_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HAP9918156.1"
                     /translation="MSCYLITGGAGFIGSTLANHLGEEHVVIVVDDLSMGKVENLDMT
                     KNITFIEGDVAEKSLMEEIMKANKFDYIFHLAAVASVADSVARPVETHRVNFESVLMI
                     LELIRKYQPDLKRIVFSSSAAVYGDEPTLPKKEESVIRPLTPYAVDKFAAEQYVLDYC
                     HLYDVPGSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQIVGEATSFTLFGDGSQSR
                     DFVYIDDVIQALLLVANEEAALGKQFNVGTGKATTLLELIHAINEILGVELTLEYKEE
                     RSGDIHDSLADITKIQSIGYQPKYGILSGMTNYLKTEIN"
BASE COUNT        46465 a        23450 c        29314 g        42143 t
ORIGIN      
        1 aataaaaaca ctaataaagg atagtacagt ataataaaaa ataaaaggta tctggtacga
       61 aaatcaacta gatacctttt ttgtgttctt atttttttgt tattttataa aaaatagaga
      121 tctcatgtta gaaaacctaa catatgcagt tgacaatatt ttttttaatt ggtacacttt
      181 agccaactta taaaacgaaa ggggacgtct gatgggagaa aaggaattgc gccgatctat
      241 gtccgtattt ccaattggta cagttatgaa gctgactgat ttgtctgctc ggcaaattcg
      301 ctattatgaa gaacaagatt tagttcatcc ggaacgaagc gaaggaaacc gacgtatgta
      361 ttcgctgaat gatattgata tgttgcttga gattaaagac tatctttcag agggattgaa
      421 tatggctggg atcaaacgtg tatacgaaat ggcacaggcg aaaaaacaag aagcaaaaaa
      481 caagaagccg ttgacagatc atgacgttcg acaaattttt cgtgatgaga ttttggcgca
      541 aggtgggtta agcaaaacag gtcattatca gtcgcaagga ttgcaaagac aattctttga
      601 ctgaataaat taaaaaagga agagggatac catggtaaag aaacagataa caggtgaaga
      661 aatcaaacga atcattgagg aagaaaacgt acgattttta cgattgatgt tcacagacat
      721 cttaggtacg atcaaaaatg tagaagttcc agtgagccag atcgacaaag tattagaaaa
      781 taaaatgatg tttgacggtt cttcgattga aggatttgtt cgaattgaag aaagcgatat
      841 gtatttgtat ccagatctct ccacttggat gatcttcccg tgggaaagtg ctcatggaaa
      901 agttgctcgt ttaatttgtg atatctataa tccggatggg acaccatttg ccggcgatcc
      961 tcgcgggaat ttgaaacgag cattgaaaga tatgcgcgac cttggtttta cctctttcaa
     1021 tttaggacct gaaccagaat tcttcttatt taaattagac gaagacggtg ggatcacaac
     1081 gaccttgaat gataaaggtg gttattttga ttttgcacca acagatttag gtgaaaattg
     1141 ccgccgtgac atcgtgttgg aattagaaag tctaggcttt gaagtggaag cttcacatca
     1201 tgaagtagcg ccaggacagc atgaaattga ctttaaatat gcagatgtgg tagatgcttg
     1261 tgataatatc caaaccttca agctcgttgt caaaacgatt gcacgcaagc atggacttca
     1321 cgcgactttc atgccgaaac cgttatttgg gatcaatggt tctggtatgc attgcaatat
     1381 gtctttgttc aatgatgaag gaaatgtttt ctatgataaa gacggtgaat tggaactaag
     1441 tgaaacagct tatcatttct taggtggttt gctgaaacat gctcgtgcct acacagccgt
     1501 ttgcaatcca acagtcaatt cttataaacg tctcgttcca ggttatgaag cgcctgttta
     1561 tgtcgcatgg agtggccgta atcgttcgcc attagtacgt gtaccagaat ctcgcggttt
     1621 atccacacgt ttggaattgc ggtcagttga tccatctgca aacccatact tggcaatggc
     1681 agtattgttg caagcaggac tagacggtat ccgtaatcag atcacaccgc cagcagctgt
     1741 tgaccgtaac atttatgtaa tggatgagga agaaagagaa gctgcacata tccaggactt
     1801 accttcaact atccataatg ctatcaaaga attgcgtaag gataatgtaa tgattgacgc
     1861 attaggacaa catatcttct caaattttgt tgaagcaaaa cgtctcgaat gggcggcatt
     1921 cagacaaact gtttccgagt gggaaagaga acaatatcta gaactttatt aaaacaggaa
     1981 taccgtgaat tagaggctgt gaaatcagcg ttttgacctg agtattagag aaactaacgg
     2041 aaaaatagtt cctcatattt tgccgttttt tgagctaata ccgagggtca gctgattgaa
     2101 caccgtttag tggagaggtt gtgaaaaagc tgccctgcaa caagtaataa gaatagccaa
     2161 gcaaaaacag cttttcttgt ttttgaattg gctgaactta ttaccgcaga tgcaagcttt
     2221 tgaacaccgt ttaccaagag gttttgaaaa agacgtttag tttcgagtat taagaaaaat
     2281 tttgtgaaaa tggctttcta catttttgca taaatttggc ttaataccga gaaactggct
     2341 tttgaatacc gtttatccag aggttgtgag cctaccattt aactccgaat cacaagaaat
     2401 aatttcaaaa acagcttctc atgtttgatg gatattgtga cttgtgaccg aggagttggc
     2461 aggagaacac cgtttattta gaaataagga ggcacgactt ttgtcgcatc tccttatttt
     2521 ttacttatgt gattcgttac cttcaatgaa tccctacaag gcacttttgt tgaaaaaaag
     2581 gtcatctttg aaaaaattag tggaattgtt ttgtttttga tattatttaa tagaaaagat
     2641 atgccaaaaa tccgagaact gtggactacg ttttttacgt aaaggaggta aataagggtg
     2701 aaagaaaaag attgtttcta cgataagcta tataaactgt tgctgcaagt aggaaaaagt
     2761 agttctttgt taggtgggat attatctatc ataatgtttg ttttattttt ttatctatta
     2821 tttcacttaa aatttttcag taacgatttt ctgatttgct taggtttaac gttagctatg
     2881 tttatatcaa gcttattatg tgggttaaat tttttttgca ctcataaagg acttaagaaa
     2941 taatttctca aaggatatcg aaaaagaaaa aattgtaagc ggatagtatt catttgagta
     3001 cttgactttc ccttagtcag cttgtattat acaccttggg caccctttta gagggtcaga
     3061 agtttacaga aatttcggct ccctattctt attgaagaat agggagcttt gtttattgtc
     3121 tgagactttt cctgcacata aattaaaaag gagtttttga acttatgaca aaatatattt
     3181 ttgtgaccgg cggtgtcgtt tcatcaatcg gaaaagggat tgttgcagct tctttaggac
     3241 gcttattgaa aaaccgcggc ttaaaagtaa ctatccaaaa atttgatcca tacatcaacg
     3301 tggatccggg gacgatgagc ccatatcagc acggagaagt attcgtaaca gatgatggtg
     3361 ctgaaacaga tttggatctg ggccactacg aacgttttat tgacatcaat ttgaacaaat
     3421 actccaacgt gacgacagga aaaatctatt ctgaagtttt gcgtaaagaa cgtaagggcg
     3481 aatacttggg agcaactgtt caagttattc ctcatattac aaatgagatc aaagaaaaaa
     3541 tcatgcgagc agctaaaatg actgattcag atgtgatcat tacagaagta gggggaacag
     3601 taggggatat agaatctttg ccattccttg aagcacttcg tcaaatgaaa gcagatgtcg
     3661 gtacagataa tgttatgtat atccatacaa cattgattcc ttacttgaaa gctgctggag
     3721 aaatgaaaac taaaccaacg cagcacagtg taaaagagct acgtggttta ggtatccaac
     3781 cgaatatttt ggtggtacgt acagagcaac ctgtttctca aagcgtgaaa aacaaattgg
     3841 cacaattttg tgatgtcgaa ccagaagcag ttatcgagtc gccagatgta gatacacttt
     3901 actccatccc attagtttta caagcacaag gaatggatca aattgtttgc gaccatttaa
     3961 aactagatac accagaagca gacatgacag aatggaaaga attagaagaa cgtgtcttga
     4021 acttgaagaa aaaagtacgg attgcgcttg tcggtaaata tgtcgagtta ccagatgcat
     4081 atatctcagt agttgaagca ttgaaacatt ctggtttcgc ctataattca gacatcgaga
     4141 tcgattggat caaagcacaa gaattaacga gagaaaatgt agaagaacgt ttgaaagacg
     4201 cagatggaat cttggttcct ggtggatttg gtgatcgcgg agtagaaggg aaaatcgaag
     4261 cgattcgtta tgctcgcgaa aatgacgttc cgtttttagg tatttgtctt ggtatgcaga
     4321 tggcttgtgt cgaatttgca cgtaacgtcg ttggattaga agatgcgtca tcagctgaaa
     4381 cgaatccaga tacagcaaac aatattattg atttaatggc tgatcaggaa aacatcgaga
     4441 atttgggtgg aactttacgc cttggattat acccatgtaa aatcaaaaaa ggaacgaaga
     4501 cggctgaagc atatgatgga gccgatgttg tgcaagaacg tcaccgtcac cgctatgagt
     4561 tcaataacaa atatcgtcaa ttgtttgaag aacaagggct agtcttttca ggtgtctcac
     4621 cagataaccg tttagtagaa atcgtagagc tgccagagaa aaaattcttt gtgggatgcc
     4681 aattccaccc agaattgatt tctcgtccaa atcgcccgca aaaattaatc aaagcatttg
     4741 tcggagcatc attagcagat cacgaaacaa aataattttt gaacttgata gaaagagaaa
     4801 agtagcaaga gaaggggata agcaattttc ttgtcacttt tctctttttc tgatttgtta
     4861 ttatttgtag gaaaaaagat agaaagcaaa agactttttt cgattaaagc gctattggat
     4921 attttatttt ttgcaaaaaa cttcttgaaa attttcattt tcattgattt tagtagaaaa
     4981 ctaccgaaat gtttggtaaa atagggtcgt tggttaagaa ttgtcttaac aaatttagtt
     5041 ttacaaaact taggaggaat tttattatgc cagtagtatc aggagctgaa tttttaaaag
     5101 ctgctcgtaa aggcggttac gctgtcggcg gattcaacac aaacaacttg gaatggactc
     5161 aagcaatcct tgaagcggct gaagcaaaac aagcaccagt acttatccaa acatcaatgg
     5221 gtgcagctaa atacatgggt ggttacaaaa tctgtaaaga tttaatcact gatttagttg
     5281 aagcaatgaa catcactgtt ccagttgcta tccacttaga ccatggtgat tacgaagctg
     5341 ctttggaatg tatcgaatta ggctatactt cagtaatgtt cgatggttca catctaccat
     5401 ttgaagaaaa cttgaaatta gctaaagacg ttgttgaaaa agcacatgca aaaggtatct
     5461 cagttgaatg tgaagttggt tcaatcggcg gagaagaaga cggaatcatc ggtaacggcg
     5521 aattagctga tattgaagaa tgcaaacaaa tggttgctac aggtatcgac tacttagctt
     5581 gcggtatcgg taacatccat ggtcaatatc cagaaaactg gaaaggatta gcttttgacc
     5641 acttacaagc aattgctgat gctgtaggtc cagacatgcc tttagtatta catggcggat
     5701 caggtattcc tcaagatcaa atcgaaaaag ctatctcaat gggtatctca aaagtaaatg
     5761 ttaacactga attccaatta tcatttgctg cagctactcg taaatatatc gaagctggta
     5821 aagataaaga aggtaaagga ttcgaccctc gtaaattatt agctccaggt aaagcagcaa
     5881 tcgttgaaga tgctaaatct catatcgact ggttcggttc agctaacaaa gcgtaagcat
     5941 ccacataaca gatacgatat ataggaatgg agaacgactt ttgaagtcgc tctccattct
     6001 tttttactta aaacggcaga ccatgctcgt ttcttttttt tatctatgat aaaatacaag
     6061 catatccatg aaaaaagatt aataaacaag gtggaaacag atgaaaaaaa tcgtaatcaa
     6121 aggaaaccga cctttagctg gtgaagtaac gatcagcggt gcaaaaaata gtgcagtcgc
     6181 attgattcca gctgcaatct tagctgattc cccagtgatc ttggaaggtg tgccggacat
     6241 acaagatgtt cattcattaa ttgaaatttt agagatcatg ggcgcaactg tccatttttc
     6301 caacaacata ctagaaatcg atcctcgaga agtcgtatcg attccgatgc cgcacggaaa
     6361 aatcaatagt ttgcgtgctt cttattattt tatgggaaca ctgcttggta aatttggcga
     6421 agcggtagta ggcctgccag gtggctgtta tttaggccct agaccgattg acctacacgt
     6481 caaaggattt gaagctctag gtgctgaagt gacaaatgaa catggtgcca tgtatttacg
     6541 gacagaaaat aaaggattgc gtggcaatcg gattttcatg gacgtggttt cagtcggagc
     6601 aacgatcaat gtgatgttag ctgctgtaaa agcaaaagga cagacagtaa ttgaaaacgc
     6661 tgcacgagaa ccggaaatca tagatgtggc aactttactg aataatatgg gggcaaaagt
     6721 tcgaggagcg ggtacagatg tgatccgtat cgaaggagtc gaaacattac atggctgtcg
     6781 gcatttcatg attcctgacc gcatcgaagc aggaacctat ctagcattag cagctgcagt
     6841 aggaaatggt atcaaagtga aaaatgtgat ctttgaacat ttggaaagct ttatagcaaa
     6901 attgcaggaa attggtgtcc atatgaaaat cagcgaagat gaaatcgaag tttacccctc
     6961 gaaagaatta aaacccgcaa acatcatgac ctatccttat ccaggatttg ccacagatct
     7021 gcagcagcca cttactgcgt tactattgat gactacaggc acttcagaaa tcatcgatac
     7081 gatctatgcg aaacgtgtga accatgtgcc ggaactagca cgaatgggcg ctgatattac
     7141 cgtggaagga aatatgatca tcgtgaatgg tccaaataaa cttcacggca cagaagtcgt
     7201 ggcttctgat cttcgggcag gtgcttgttt agtgattgct ggactattgg cagaagggac
     7261 aacgacgatt tacaatgtag attatatctt acgcggttat gatcatatta tcgagaaact
     7321 aacagcctta ggggcatcaa ttgaaatgat agaggaagtg ggagctgaat gagcgattat
     7381 ttgacaatgg cagaactaga gaacaaaacg ttaaaagaag tttatagtta tgccaaagaa
     7441 tttaaaattc cttattacag taagatgaat aaaaaggaat tatctctagc tgtgattcgt
     7501 gcacaagcag agaaacaagg attttattat atggaaggaa tattggatat cgttggtcaa
     7561 gatggttacg gttttttacg gccaatcaat tatggaccaa gtgcagaaga tatctatatt
     7621 tctgcctcac aaatcagacg attcagtttg agaaatgggg acaaagtggc aggaaaagcc
     7681 cgtccaccta aagaatcgga acgttattat ggtttgatgc acgtggaaag cgttaatgga
     7741 aaagatccgg aagaagcaaa agaacgtccg cattttccag ccttaacacc tctttatccg
     7801 caaagacagc ttcaactgga aacaacaccg caaagactat ctacccgaat gatcgatatc
     7861 ttttctccaa tcggttttgg tcagcgtgga ttgatcgtcg caccgccaaa agcagggaaa
     7921 acttctgtat taaaagaaat cgcaaatggt atcaccgaaa actatccgga agtagaatta
     7981 atcgttctac tgattgatga acgaccggaa gaagtaacgg atctagaacg aagcgtgaaa
     8041 ggcgacgtgg tatcttctac atttgatctg cagccgcaaa accatactcg agtttctgaa
     8101 ttagtattgg aacgcgcaat gcgtttagtc gaagataaac gagatgtcgt tatcttgatg
     8161 gatagtatta ctagacttgc tcgggcctat aacttagtcg ttccaccaag cggacgtaca
     8221 ttgagtggcg ggattgaccc agccgcattg tataaaccta agaaattttt cggggcagca
     8281 agaaacatcg aagaaggtgg cagtttgacg attttagcga ccgctttagt cgatacagga
     8341 agccgtatgg atgacgtgat ctatgaagaa ttcaaaggaa ctggaaatct agaattacaa
     8401 ctttcacgtg aattatctga acgtcggatc ttcccagcca tcgatatcaa aaaatcaggt
     8461 acccgaaaag aagaattatt gatgccaagt gaagaattag aagaaatttg gaaacttcga
     8521 aaaaatatga ctggagattc attagaatat acggaacaac tgatccgctt tttgcgtaaa
     8581 acaaaaaaca atcagcaatt tttcaaagaa tttcaagatg tctcctttgg taaaaaaaat
     8641 ccgaccagaa atctgaaaag ataattgcaa ccttgactga aacatgttaa gatgttaatg
     8701 ttgtaaatcg acaaatcaac tctggttcag caaatggagc agggcaaagg agcgaaaaaa
     8761 tcatgaaaga aaacatccat ccagattacc acccagtagt attcatggat tcaacaactg
     8821 gtttcaaatt cttgtcaggt tcaacaaaaa cttcacaaga aacagttgaa tgggaagacg
     8881 gtaatacata cccagtcatc cgtgtcgaag taacttctga ctcacatcca ttctacactg
     8941 gacgtcaaaa attcacacaa gcagacggac gtgtggaccg tttcaacaaa aaatacggtc
     9001 tcaaagacgc aaacgctgcg gaataataaa aacgctgata actcaacgtt tagcagacta
     9061 tctgataggg atagtctgtt tttttatgtt ttttgccaat ttaaaacttc gtttgatgct
     9121 gaaaaagtcg taggatcata atgcacaaaa caatgaaaaa ctagtatata gttttaatta
     9181 tccacttgat aaaaacacct tatattaacg aaatgaagag gggttatttc gaagtaaaaa
     9241 accatttacc taaaattaga agctaagctt tcctttcaag gaaggctttt tgttttgtta
     9301 tagacttgcg attgtttgtg agctagatcg ttcattttaa tgacttatac acgcggatga
     9361 tgggtctttt tcctctgttc attcataata ttgaacggta cagctagcga tgctatactg
     9421 acctcagttc caagaaaacg ttaacatttt ttgtggggga gaggtttttt atgaggtttg
     9481 atcaattgat cgcagaagaa ctgattgttt tcgatccatc gatagattca aaaggatcat
     9541 tatttgagcg aataggggat gtgttgaaaa aaacaaataa ggtaagaaat gttcaaaaag
     9601 tcgttaaggc actattcaaa cgagaggagg agatctccac aggaatagaa gatggatttg
     9661 ggattcccca tgcaaaaagc aaatatgtga caaaaccagc actgtgtttt tttcatacag
     9721 ccaaattaac ggactatagc gggatagatg aaatgccaat tgaatatatt tttgccatcg
     9781 tagttcctga aaaatcagca gatatgcatc tagagattct cagcacactc tccagacgat
     9841 tgatgaatgc agattttaga aagggaataa aagaagccac tacgccagca gatatcatca
     9901 gacagattag tcaagaatga aaatagtaga gtctttgtcc gagtatcgat agtgaagaaa
     9961 aaggaggaaa ataatcatga aaatcgttgg tgtaactgct tgcccgactg gaattgctca
    10021 tacttatatg tcggcagaaa aattagaagt aacagcaaga aatctaaacc atcaagcaaa
    10081 attcgaaaca caaggtgtaa aaacagaaaa tcagttatct gaacaagaaa tcaaagaggc
    10141 agatgcaatc attcttgcag tagataaaga aatcgaattg gaccgatttg ccgggaaaaa
    10201 agtaaagcgt gtctctacaa gccgagcaat caaagaaccg caagtagtaa tagacgaagc
    10261 attgagagat attggcgtat tcgttgtgtc taatgaaaaa gaaccagctg caaatgagaa
    10321 aaagccgaca atctataatc atttcatgaa tggtgtgaat tacatgcttc catttgtgat
    10381 cgcaggcgga atcatcatcg cactgagttt tgcctttggg attacagcgg ctgatcccaa
    10441 ctcagcagac tataatgtgt tagcagctgc attttcaagg atcggaggag atacagcgtt
    10501 tgccatgatg gttcctgcgt taggcgcggg gattgctaca tcgattgctg gaaaagcagg
    10561 ttttgcccca ggaattgttg ccggattact ggcctctaca ggtggttcag gttttcttgg
    10621 cggaatgatt ggtggcgtgc tagcagggta tatctgtgat tttctggcaa ataaaattag
    10681 cgtaaaaaaa gaattttctg ctatttatca gctgattgtc gtcccctttt tatcgatctt
    10741 aattattggg cttttgatgg tatttgtgat tgaacaaccg atcacatggg tgttggatgc
    10801 tttgacaaat tggctgaata gcttgggaaa tacatccggg ctattgttcg gattacttat
    10861 cgggatcatg atggcagctg atatgggtgg gccgatcaat aaatcgactt cgactttctc
    10921 gatcggtttg atgtcagctg gagtaaatgc accgattgct gcttgtatgg ctgcaggaat
    10981 gacacctccc ctaggtattg ctttagccac tttattattc aaacataaat ttaccaaaga
    11041 agaacggact tcaggtcaat catgctggat tttaggcgct tcgtatatta cagaaggtgc
    11101 tataccgttt gcggtatctg acccgatccg cgtgatacct agtttgatga caggttcagc
    11161 agtcgctgca gggatttcca tggcagtagg agttacttca atggcacctc atggagggat
    11221 ttgggtgatg ctaattccaa atgttatgaa caatttacct atgtatgtct tggcaattgc
    11281 atcaggtaca gtcactacag cctttatgct tggactattg aaaaaaacag tgaaagagaa
    11341 aaaacaagac aagtggacga aaaatgaaaa tctaacaaat gaggtggaag cgtaatgtta
    11401 tatacaatga aagagttatt gaaagtggct aaagagaatc aatttgcagt accagccttc
    11461 aatatttgca gctatgatat gatgaaagca atcatcgaag aagcagagag attagaagca
    11521 ccagtaatct tagaaattca tcctgatgaa atcgcctatt taagtgatga gtttgttgcg
    11581 gcagtaagag cctatgctta tacaagtaaa ctgccgatcg ttatccactt ggatcatggc
    11641 ggtacgatag aagatgtctt acgggcgatt cgaaacggct attcttccgt gatgatcgat
    11701 gcgtctttga aatcattcga cgacaatgta cagattacga aaaaagtggt tgcgttagct
    11761 catgcggtaa atgtctcagt ggaggctgag cttggaacga ttgggaataa tggttctgct
    11821 gaaggaggtg ctgaaacaat catttataca gatcctgatc aagcgaagat ttttgtgaaa
    11881 gaaacaggaa tcgatacact tgctgtagca attggaacag cgcatggctt gtatccgaaa
    11941 aacagaactc caaaattaaa tatcgactta ctgaaagaat tgaaccaaca aatcgatctt
    12001 ccatttgttt tacatggcgg ttcaggtaat ccggacgcag aagtcagcga agcagtaaaa
    12061 tacggagttg ccaaagtgaa tttgagctca gatcttaaaa gcgtcttttt caatgctttg
    12121 agagaaatat tgagtgaaaa tccatcgatg tacgagccga atgcaattta tccagcagct
    12181 aataaaaagg tacaagaagt ggtcaaacat aaaatggaaa ttttgaacac agtaggcaag
    12241 gctagtttat actaaaaata aaaatgtgtg gtcaagaggg tatgacaata gtcttgtcat
    12301 accctcttaa ccacacacga tgttcagaag tagttttttc gaaaaatttc ttcttatttc
    12361 tcgaagccaa tcacttctgt cacaacctct tttttcctgt tactatataa agtaaatggg
    12421 gtgattttat gttgaatgaa gtggagaata gacagcagga gattgtgaat tatctaaaaa
    12481 acaaccaatt tgcaagagtc accgatttga tcgagttagt aaattatagc gaagccacga
    12541 ttaaacgtga ccttgttttt ctggaaaaaa gtggattgat ccgtagagtt cgcggtggcg
    12601 ccatgcttat tgacaatcga aaaattgacg tgccttacct gatgaagatc accaaattag
    12661 aagaagaaac agaaaaacgt aagattgcag atacagcttc ttctttgatt cgagatgata
    12721 tggtcttatt cttggattcc agtacaacct ctttacactt agtcagaaac ctagcaagat
    12781 ttgatggttt gcagatcatt acgaatggca tcgtcacagc tgctatgttg agtgaatata
    12841 caacggcaaa agtatctgta ttgggagggg caattgttcc gaaacgagca acaatcaacg
    12901 gttcaaaagc ctataacgat gcattgtcat attatgcgga tttagcgata atgagttgtc
    12961 gcgggctaga tttcagccaa ggagtaacag aaactcatga aggagaggca atggtcaaaa
    13021 aagcttttag aaaacaatca gaccggcttg ccgttttagt tacacagcaa aaaattgcac
    13081 ataaattcat gcatcaagcc attgcttttc atgaaatcga ttatctggtg acaagtaaag
    13141 aactgacaaa agaacagtat gcgaaagtaa aggaacacca tatcgaatgt ctgtacggat
    13201 aaattattac gtgaatgttc taagtggctt cctcattttt agataggacc ttatctttgc
    13261 tcgcttctct cagccgatgg tataataatg aaaaggttat cttgttagat aatcttactt
    13321 ttttatccag gagaaaggag tgcagaaaat gaaacatgtg aaggcgttgg tagtgaaggc
    13381 aatcatgatc tgggcgattc tgtgggtcgt gctgacaggt ttatacggtg ttagctttat
    13441 ggattcaacg attgttgggg taattattgt agtcatgatc tatgtgctag gggatctgtt
    13501 gatcttacgt aaagtaggga acatcgcagc gacgatcgca gatgctggtt cagctgcagt
    13561 tatcttgtgg ctctatctat attttatgaa tgatacagtc gatatttgga tgaaagtatt
    13621 gattcctgct ctattgatcg gtgtggcaga atggttcttc cacaagtggt tgctaagtca
    13681 aggcatcgta ccagatgaac ggaaaatgga ataaatcaac agttaaacta ttcttatcga
    13741 taaatgaaag ctttaaaaat aaatagatgt ttaattaaga ataatcataa actattttta
    13801 ctcaaaaaac cgctcagatt attttcagat ctgaacggtt ttttttatct attcatcaag
    13861 atcaaatttc atttttcaat gattcaatca attcgccctc tgcttcatga ctatgttttc
    13921 ggccattaac aagatagagt gctgtacata tcaatggaat gactccggca atcacataca
    13981 atcctctaaa tgaagtaaga gattttagca tccccatcaa ataaggacca acgcctaagc
    14041 ctaggtctaa accaataaag taggtagata aagcaatact gattcgatgt tcgcggacga
    14101 tttttaaaca aacagcctgt ccatttgaca taaacgtacc ataacctagg ccgactaatg
    14161 ctccagagat aagcaatgtc acactattcg tcgttacact cagtaaaaat aatcctgctg
    14221 ttaagaataa atagctagga tacatcacgt attgttctcc tttgacatcg aaaatccttc
    14281 cagtcaatgg acgtgtaaat gtgatgacca aagcatatac gacgaagaaa aatgacccag
    14341 cagcaactaa gtggattact tttgtataag aagataaaaa tgataaaaca cttgaataag
    14401 aaagacccat caaaaaggca attcctgtaa tgaaaagtgc tttcttttca ataaaactat
    14461 tgatattcca tgagcgaaga gatatttttt gttcttcaga taatggaata ttttttacag
    14521 gaaagacaag gcaggcaatc gtaacaaaga agaccagtac aattgaaaac cagataatga
    14581 aacgaaaatc agtcatattc agcaaaatca ttcctaaaaa tggcccaaca gcggcagcta
    14641 aactagtact taatccataa taattgatgc cttccccttt tttagatgca gggatatatg
    14701 cagtaacgat agcatttgta gctgtagaaa ctgtaccgta gccgaatcca ttcaagaatc
    14761 gaactaaata caatgccccg atagttggcg tataaagata tgctaatgtt gaaaggagat
    14821 agaagatcgc gccgcctctt aaggtaaact tccgaccaaa tagttctaac tgtttcccca
    14881 tgaataagcg tgccagcaat gttccgatga tatagatccc agaagcaaaa cctgcttggc
    14941 tcaagctggc gtgtaaggtg tcttgcgcaa tgactgccat gatgaccatc aacagataat
    15001 aaatcaggta gacaataaaa ttgatcaatg tgattccaat aaaatattta ttaaataatt
    15061 tcttttccat gtatgttata cattcccctt aagctgaata tgcttttaat ttttgtattt
    15121 gaacttctac atcgtgataa ttggatggat agcgatcaac aagttggcga ataaataaat
    15181 ctcttgtaga cataaacaga tttaaaaatt cttcgcattc gtttgttgga ttctcatcca
    15241 ctgctttgat caattcgatc gagttttgat acgcatcatt taaaaagact aaagaagctt
    15301 tcatatcaaa tcttgtttga tatgctgttt ctaatcgatt aatgattgtg ttcatccagt
    15361 aattcatgtt ttttccctct attcttaaaa aattttactt ctgcagtata acgaataaaa
    15421 aaaaggaaaa aaatcgcata agtgatggaa aaaggtactt aacaaatgtt aatatcatta
    15481 gtaaaatacc tttggacttt aaaaagtttt aaagtactgt atgatctttt tagatgaaag
    15541 tttggatggg ggagcatcat tggatagtag agtcttaaag gaatatttgg ataaaaatga
    15601 ttttccgatt atacgcaaag gatatcataa atatttgaca ttcgaaggga tggaagattc
    15661 ccatacgtat atcttgaaag agggaacggt aaaagcaagt gttatttcaa atgatgggag
    15721 agaatttaac cttcgctata tcaaagatct tgaaatcgtc tctcttttaa aagacgaata
    15781 ttcacaattc atcgattcgc cgtataatat tcgagttgaa tcagaatatg cagagtttta
    15841 tcgaatcaat cgtgtgcgct tttggcaaaa tatcaaccaa gacccaaagc tacaacacta
    15901 tgtaaaagag tattaccgct atcgtctgct ttattcgatg aaaaaaatgc agcaaatgct
    15961 attaaacgga aagctgggag ctgtttgtac gcaactttac gaattatatg atttattcgg
    16021 tgtgcggaca gaagaaggga tgctgatcga tttttcgatc acgaatgaag aaatcgccaa
    16081 attttgtggc atctcaactg ctagcagtgt aaacagaatt ttaaaacaac tgaaaaaaga
    16141 tggtattatc accgttaaag accatcatat cgttattttg aatatggact tgttagaaga
    16201 caatattatc ttttgatgaa caaagaggtt gtgaaatcag cgttttgacc tgagtattag
    16261 agaaacaaac ggaaaaatag ttcctcatat tttgccgttt tttgagctaa taccgagggt
    16321 cagctgattg aacaccgttt accaagaggt tgtgagcctg ccgtttaact ctgagtcaca
    16381 aggaacaatt tcaaaaaaca gcttttcatg tttgatgaat attgtgactt gtgaccgagg
    16441 agttggtagg agaacaccgt ttaccaagag gttgtgaaaa agctgttctg catcaagtaa
    16501 taagaagagc caagtaaaaa cagtctctta tgtttttaac ttggctggac ttatcaccgt
    16561 agatgtaagc ttttgaacgc cgtttatttg caacaaaagg ggctgtgaca cgatttttgt
    16621 cacagccctt tttttgcgaa cattcattta gcaattttta gaaacttctg ccgccaccgc
    16681 caaaagtccc gccaccgcta gaatgggtag tacttccgcc accgccgctt ccaggaggtg
    16741 ggcttttggg gattcttctt gtcgtgacaa aagaatttgt cagacgatcg gttttttcag
    16801 ttaatttgat actggatttt tctcggaatg gatatttgta gatgcctgat ttcaattgat
    16861 aacgcgaaac agtcacaaaa taaaagacaa gactaaggat aagagccaaa acaacagcaa
    16921 tgatgatttc tactgtcgtc aaaaccttat agcgtgtaat tttgccagtt tcttcgtcta
    16981 cacgataatg tcctcctgga actccgtctt ccacatatgt tgctgattta gacaggtaag
    17041 cttttgcagc attgtaatag gcagcatttg tcatctgatc ataaacatca tctaagatcg
    17101 aatcgattct gctatcagtc aaataatcga tcatattacc agaagtcgaa atgtagattt
    17161 cacgctgtcc catatctaat agcaatactg agccattttg atcatttccg actgcgtctc
    17221 ttaagtaatt atcagcaaag tctcttggtt cttctgaatt agatgtagtc gttacgatca
    17281 agactcgtcc tttgattttt tcattcagct tttctgcctg ttgttccaaa gattggattt
    17341 gatctgttgt gaataaccca gcctcatcat ttaccgtagc tgtatctgca tgagtagcca
    17401 ccggccagcc aacacttact agcaaaagaa agaataaaaa aaactttttc atcatatcaa
    17461 gtaacctccg atcagaaaga ggatcgctag cccagcgaaa atcccaagag cggtcagtcc
    17521 caattttttc tggctgatcg gcaggacacc gctgaccttt cctgtttgtc cattcattgc
    17581 gtagtagtag acttttttac ttgagtcatt gcttcgataa gtaacaagcc ataccggcag
    17641 taatacgtaa ttatttactt cgtttgtgat tgaagcagaa gttcgcacac cggtcaatgt
    17701 tgtatatcca tttgcggtat cttttaacag cttctctgaa tagtctttta attcagattg
    17761 aatctgtgat ttgattgcct catattcgat atctcttttt tctgcttgga acccggctaa
    17821 atactggctt ttaaaatcaa ttgctttatc taacgggaaa ggttgcacgc cttcgaccat
    17881 cttttgttgc gtattttttg ataaggcatt ttttactagt tctttaaaag aaagcttgcc
    17941 tgctcttgaa acagcaaatt gcttcgtttc tgtgtattcg atgtcaccta tccgccatat
    18001 gcggataact gtaccattcg cctgcatggc accatctact tcagcatctg tagcccaata
    18061 agggaaatac acacctgtca acttatcgat ttgatcctta ttgaaaaaag ctttgggaac
    18121 aaaccatttt ttcttggtcc aagcgagaaa tctgttgatt gcttcttctt tctcgacagc
    18181 aaaaggaagt actttatttg gcaagaattc tccgcttaaa cgtcccgata gtacgacggg
    18241 attatggcag tagtaacaat aagtcgcagc agttgtcgca tcagtcacga tctctgcacc
    18301 acagttggga caaagaaaaa gatctatcgc tgcggtatta tctgcagatt cgtccgctgt
    18361 atctgaaaaa ccgcctgcct cgtcaaccgc tttgcgctcg gcttcattca tatcggcaag
    18421 ctgttcttgg gctgtaaaag taaaatcttc ggtaggtgaa ggttcttctt tgctttcttc
    18481 gccagccata cgcgcttctt tttgcttttg ctcatattgg ctgacttctt cttctgtata
    18541 gatattcagg cagtattcgc aatgaaattt ttggtctttg ggatcaaaag tcaacggacc
    18601 gccgcaatta ggacatttat gtgtaagaac atccatcagc tgcacacctt tctaaataaa
    18661 ttaattaacg gggattcctt taaacggttt accacagttt ggacagaatt tagggatgcc
    18721 gttggaaaga tccacgatct cattgcattc actgcatcgc atttgtagct taggctgaac
    18781 gttttctaca ggtttggcag ctccgcagtt ggaacagaat tttccggaat tttctgtccc
    18841 gcaaactgga catgtccaag tatccgcatt tccttgtgca gtttgttgat tttgtttttc
    18901 tgcttgttgt ttgatttgtt cttggttatt ttgagctgct tgagaaagat aagaaccatt
    18961 tgcattcatt cccataccta cacccataaa gccggtcatt gctcctgctt cattttttcc
    19021 ggctgcttcc ataccacgtg caatggaacc ttgcacgtat ccttcacgta cgctaggatc
    19081 acctagcatc gccccttcgt tacgcatatt gatcagtttc acagaatcat cggtgtaaga
    19141 aatgctggct acagccacag aaacaatctc catcccacgt aattcacgcc aagaatcatc
    19201 caatgcagta tccatgtatt tgcttaattc caaactctta gatggtacat aagaaatccg
    19261 ttgaccatct gcagacatct gattgattgc tgattgtagc gctgttagga attcagctaa
    19321 atactgctca ttgatatcat tgatttccac ttgtgttttg tttttaggga ttgcgtttgt
    19381 ataaaataaa atcgggtcaa cgacatgaat agaataagtg ccatgtgccc gtaagaataa
    19441 ttctgcgtta tagaaattgt caaagtagtt caacggcgcg cttgtaccga atttgattcc
    19501 ctttatttct tggagattga tataaaaaac ttgttgtttc tgcggtgtga cgccgccaaa
    19561 tttgaatcgg tcaaaagttt cagcaattgc ttctttcagt gttccattga acatggaagg
    19621 agctgcgtca tttttaatgg tgtaataacc ttcctcagcg gtataatcga tgatttttcc
    19681 accatcgacc aaaagcatca tcatgtttgg atagacatgg acgactgttc cgtctgttaa
    19741 aacatcttct gttcctttac gattggagcc acgtttgtca tctttgcgga ctttgacacc
    19801 ttttgtcatg accgttgtat cactcatatt gtctggttcg atgacttcta gccattgatc
    19861 tgccagaccg ccgccaacca tacttgttgc tgctttgata agacccataa aaaattcctc
    19921 ctctgattca gctagtgcca aacaattatt gtttatttat tataccatag gaagatgtga
    19981 aacttctcgg actagagacg gaattgaaat ggaaaattgc gtattgttta aatacctgaa
    20041 aaagccgaga aggatagtag tttttttacc taatatcacg tataataaga gatagtgaat
    20101 tttttaaaaa agggggatcc ttatggcata tcaagcactt tatcgagtct ggcgttcgca
    20161 gcgtttcgaa gacatcgttg gacaaaaagc tgtgacacag acactaaaaa atgctattgt
    20221 ttcccacaaa acctcacatg cctacttgtt tacaggaccc agaggaaccg gaaagacgag
    20281 tgcagcaaag attttcgcaa aagcaatcaa ctgtccgaac agtaaagacg gtgagccatg
    20341 caacgaatgc gaaatgtgtc gatcaattac tgctggtacg caagaagatg tgatcgaaat
    20401 cgatgctgcc agcaataacg gagtcgaaga aatccgattt atccgtgatc gggcaaatta
    20461 tgcgcctaca aaggcagcgt acaaagtgta cattattgat gaggtacata tgctttcaac
    20521 aggagctttc aatgccttgt tgaaaacatt ggaagagcca aaagaaagtg tcatattcat
    20581 cttagcaacg accgaacctc acaaaatacc agctacgatc atttcacgga ctcagcgatt
    20641 tgattttaaa cggatcaata cggcagatat tgtcgaacat cttgaattta tcttgaaaga
    20701 atcgactatc agctatgaac cagctgcatt gaatgtgatt gcccgttctg cagaaggcgg
    20761 gatgcgggat gcattgagta ttcttgatca ggcaatctcg tttagtgaag gaacaattac
    20821 tttagatgat gccatgcaag tgacaggtag cttaactgac gaaatgatgg acagttactt
    20881 gagtgcttgc gtgaaaaaag aagtaccaga agcattggaa acattgaacc aaattctcgc
    20941 agccggcaaa gaatcacgtc gctttctaga ggatatgtta caatactgcc gtgacttgct
    21001 gatgtatcag caagcaccta acctagttga ggaacaatcg agtacgatca gaagtacatt
    21061 tacagaattg gcacaaactg tttcagcgga tcagctgttt tcaatgataa agattttgag
    21121 cgatactcaa aatgaaattc gttttaccaa tagtgccgct atttatttgg aagtcgctac
    21181 agtcaaactt gcacagctac cgtctgcttc tgttccttca gcagatgtag catttgcaga
    21241 tgatcagata gtaaaacagc tacaacaaga gattaaggaa ctaagaaacg aaatcaagga
    21301 actacgcagt aatacaggaa ttgagaatac aaaagtacag ccggtacagc aaacaaatca
    21361 taaaaaacag acaagtacct atcgtgtacc gacggaacga gtatataaag ttctgcggga
    21421 agcgacaaga cagcatctga accaagtaaa agaagtatgg gaagacttgc tgatgagtct
    21481 atctgtcact cagcgagcaa tgcttaaagc aagcgaacca gttgcagctg gacctacagg
    21541 actagtgatt gcgtttgatt atgagattgt ttgtcaaaga gcagcaaatg acgaagaatt
    21601 gcagctttcg gtacacaaca acttaagccg aatgatcaaa gattatgcgc ctgatcctgt
    21661 ttatattaca agagaaagct ggccagaact tcgaagaaat tatttgacgc aggcacaaga
    21721 agagggaaca gaaaactctt ttgcttctga agactcagaa ggagaaatcg agttgcttcc
    21781 tcaagaacaa gaagtaccag aagaaacact tgtagtaaaa gcagaggaac tgtttggctc
    21841 cgaagctgtt acagtggtag atgattaata aaaaggagta gataattatg atgcgtggaa
    21901 tgggcaatat gcaaggcatg atgaaacaag tacaaaaaat gcaaaaagaa atggcagaag
    21961 cacaagagct gctaaataaa acagagttta caggtaaagc caccaatgaa ttggtcaaag
    22021 cggtttttac aggcgatcgt cgcatgaaag atatccaaat caatgaagaa gtcgttgatc
    22081 ctgatgatat cgagatgctt caggatcttg tcgtaatggc agtcaatgat gcgatcgcac
    22141 aaattgatgt ggaaactgag aaaaaattag ggaaatacgc aaaaggtttg ccaggatttt
    22201 agttttaaac tgaaggagta atgaatatgc aatatccaga accaattgcg aaactgatcg
    22261 acagctacat gaaacttcca ggaatcggtc agaaaacagc gacaagactc gccttttata
    22321 cgatcgatat gaaagatgaa gtagtaaatg aatttgctaa atctttgtta agtgtaaaac
    22381 gcgatttgca tttttgtagt atctgcggaa acatcacaga agaagatccc tgcgaaatat
    22441 gtagaaatcc ttcacgtgat cgaagcgtta tcctagtggt cgaagagccg aaagatgtaa
    22501 tggcaatgga aaaaatgcga gaatatcacg gactttatca tgttctccat ggtgtgttat
    22561 caccgatgga agggacaggt ccagaagaca tcaatatttc ttctctgttg cagcggctgc
    22621 atgatgcaga tgtaaaagaa gtgattatcg caacaaatgc aacaacagag ggagaagcaa
    22681 cagctatgta tctctcacgt ctgatcaaac ccgcaggtat caaagtgact cgtctagcac
    22741 atggtctgtc tgtaggctct gatatcgagt atgcagatga agttacttta ttgaaagctg
    22801 tcgaaggaag acgtgagtta taaatttcta attaagtgtt ttttcagata caatataaaa
    22861 gcagcacaag agatgggggc aacaatgtga acggactatt tattacaatc gaggggcctg
    22921 atggtgcagg aaaaaccagc atcttgaatg aactttaccc tcttttgaaa gaagtagcaa
    22981 aaacagaaat cattcaaaca agagaaccag gaggcattcc gattgctgaa gagatccgag
    23041 cagtgatcct tgatccaaaa aatgatcgga tggatgaacg tacggaagca ctgctttatg
    23101 cagcagcaag aagacaacat ctagtcgaaa aggttctgcc tgctctggca aaagggaaaa
    23161 tcgttttatg cgatagattt gtcgacagtt ctcttgctta tcaaggtgca ggccgacgca
    23221 tcggcgttac cgagattgct cggttgaatg agtttgctac agaaggtact acgccggatt
    23281 ttacccttta tcttgacgta gattcagaca ccggattaag aagaatcaag aaaaacagac
    23341 aaaatcaaat cgaccgttta gattcggaag gtttggaatt ccaccagcgt gtacgtcatg
    23401 cttacttaaa gctggcagaa gagaatccgg aaagaatcca caaagttgat gcaagaaaaa
    23461 gctttgaaga agtgctgcaa acaagttatc atacaattat tgaacagtat ccgcaatttt
    23521 ttgaaaacta ggaaggtgaa cgtttatgaa aattatttta gcgattatcc aagacaaaga
    23581 cagcaaccgt ttatcaaatg aattgattga tgcaaatatc cgtgccacaa aactatcatc
    23641 gacaggcggc ttccttaagg ctggaaacag tacgtttatc gtgggtattg aagatgaccg
    23701 tgtagaagaa gcattggaaa tcatcaaaaa aacgtgtgaa tcaagaaagc aatttgtatc
    23761 tacacctgtt acactggata tttcgatgga tgggggcgtc ccttatccag tagaagtaga
    23821 agtaggcggt gccacagtct tcgtcttacc tgttgaagga ttccatcaat tttaggagga
    23881 gctgagctat gaatcaagaa ataaacttgg ctcaaatgca accgatcgtg tatcagcagc
    23941 ttcaacggag ttttgagcat gggcgtatcg ctcatgctta tctttttgaa ggggagaaag
    24001 gaacaggaaa acatgaagta ggtatatggc ttgcacagca cctgttctgt acccaaatga
    24061 aagacaatct gccttgtgga aaatgcaaca attgtcagcg gatcgaaaag aaagaacatc
    24121 cagatgtttt ggttattgaa ccggaaggac aaaccatcaa agtcgatcag atcagacgtt
    24181 tgcagacgga attcagtcgc agcggttacg aatcaagaaa aaaagttttt ttgatcaaag
    24241 aagctgaaaa aatgaatagc agtgcggcaa acagtttgtt gaaattcttg gaagagcccc
    24301 ctggagattt tttagcggtg ttagaaacag atgcagtagg aagaattctc cctactattc
    24361 aatctcgttg tcaagttctg cattttcaag aactttcgaa agaagcactg atccatcgat
    24421 tgcaagaaga gcagattcca ttggaaaaag caaaactttt ggctttctta acaaacagtt
    24481 taggaaaagc agttgaaatc tctcaagatg aatggtttaa tgatgctaag gacttgattt
    24541 atcaatggtt cgtttacttg caaaagcaag atactcaagc ctttatctat gtccaaaaaa
    24601 aattggtaaa atcatttaaa gaaaaaacac aacagtttac aggattatct gtcttgatgt
    24661 tttattatca ggaagcacag cgtcaagcag tagcaaacgg cgaatatggc aaagtgaaac
    24721 agataaacca gataattgaa cggatattga tcgcagagca gaaactacgc agcaatgtca
    24781 gttttcaggc tgttgcggaa cagttcgttt tgcatacgat tttttagctt gccgcaaagg
    24841 atgatgatca aggaggatgg aaatggtaga agtagtaggt gtccgatata gagatgcagg
    24901 tcatatctat tattttgcac caggagactc tgaatatgtt tatagtgaca aggtattagt
    24961 cgaatctcag caatccttac atattgctac tgttgtcctt cctaaaaaag agattgatcc
    25021 caatgacctt cctgaagcta tccgtccgat cttgcataaa gcgtcacaag ctgaattaga
    25081 aaaagacaaa aaaaacaaaa aagatgctta tgatgcattg aaaatcgcaa aagaaaaagt
    25141 gcagttgcgt gagttggaaa tgaaaatggt caaagccgaa tacacatttg atcgaaataa
    25201 attgattttt tctttcacag cggacgggcg aattgacttt agagaattgg taaaagatct
    25261 ggccgctgta tttcgtactc gtattgagct tcgacaaatt ggtgtccgcg atgaagcaaa
    25321 gataataggc ggaattggtc catgtgggcg tccattatgc tgttcttctt ttttaggcga
    25381 tttcgtgcct gtatcaatca aaatggcaaa agatcaaagt ttatctttga atccaacaaa
    25441 aatttccggt ttatgcggac gcttgatgtg ctgtctgcaa tatgagagcg aagaatacga
    25501 gacagcgaaa aaagaactcc ctgactttgg aaaagtaatt gctacacctg atggtaaagg
    25561 gaaagtcgta gggttgaatc tgcttagccg tatcgttagt attcggctga ctggacggga
    25621 aaatcctgtc gaatatgaat gggatgaatt gaaagaattt atccaagtag gtgagatgaa
    25681 tggataagag atcattatac gacggattga atcaattaga atcagagttg agaaatacat
    25741 taacagaact tgttgaaatg aagcatgctt tgcatgagat cgtagaaaaa aatacaactt
    25801 tggagatgga aaatcaacgt ctgcgggaac acctgcggga attgaaccaa gttgcacaag
    25861 ctccagctga aaatgtgaag caggaacttt ctaagtctcg tatgaatctt gaaaaaattt
    25921 atgaagaagg attccatgtc tgctacgatt tatatggatc aagaagggac aatgacgagc
    25981 cttgtgcgtt ttgtctagaa gtgatctatc gcgaaagcgg tcgatgacca ataacaagag
    26041 gagactggag cggagaaata ctgctccagt ctttttatta aggagattgt gacagatgta
    26101 taatcaaaaa agtttcaagg gaaatccggt atatggggat ttatatcttg taccgacgcc
    26161 aattggaaat ttagaagata tgagctacag aagtgttcgt atgctgcaag aatccgatct
    26221 tatcgcaagt gaggatacgc gtaatacaca aaagttattg aatcattttg aaatccatac
    26281 accccaaaaa agtttacatg aacataatta taaagaacgt gtccctcaat tgattgcttt
    26341 gttaaaagaa ggaaaaacga ttgctcaagt cagtgacgca ggaatgcctt ccatcagcga
    26401 tcctggacat gagctagttt tagcctgcat aaaagaaggg atttctgtga tctctatccc
    26461 aggacctaca gctgggatga cggctttgat tgcttcaggg ctagtgcctc aaccattttt
    26521 attttatggc ttccttccac gtaagaaaaa agaacagaaa gaaacattga aaaagctaaa
    26581 agaacagaca gttacgctga ttttttatga gtcaccttat cggatcactg ctacattgac
    26641 gaatttttta gaaatttttg gaaatcgcca agtcgttttg tgtcgggaat tgacaaaggt
    26701 ccatgaagaa taccttagag gaagtacgga agagctactc gcttatatcg aagaacatcc
    26761 agtaaagggt gaatgttgtc tgcttgtaga agggaatttg ttcgaaatgc cagtgacgga
    26821 agttgatgaa gagttgggaa cgttaaagga acaagtagaa gcaaagatcg cttcaggcga
    26881 aaaaccaaat gctgctatca aagcagtagc gctgaaaaat gggctgaaaa aacaagaagt
    26941 ctaccgtcag taccatgaat tagactgaga tagtataagt tgtctataat gaaagactaa
    27001 tgaggaaatc agctactaac ttttcaaatc tcttgaacgc gaacgaatgt tcttgtataa
    27061 tgaataagag gtgaggatga tggagctgca atttccttta cataatgata catcaagaaa
    27121 aatcattcat attgatatgg atgctttttt cgcttcggtt gaagaacgag atcatcctga
    27181 gtttcgcgga catccattag taattgccag acaccccagt gacactggtg ggcgtggagt
    27241 agtgactaca gcaaattatg aagcaagaaa attcggaatt cattcagcaa tgagtgcaca
    27301 aaaagcgtat gagctttgtc cgcaagccat ctttaagcct ggcgaccatc ataaatacag
    27361 cgatatttcc aaacaagtac gggaagtatt ctatcgttat acagatattg tggaaccagt
    27421 tagtatcgat gaagcgtatc ttgatgtgac cgaaaataaa atcaattgca agtcagccat
    27481 caaaatcgcc cgaatgatcc aagcagatat ttgggagagt gtccagctaa catgttctgc
    27541 gggagtaagt tacaataagt ttttggccaa attagcatca gactatcaaa aacctagagg
    27601 aatcacggta gttgcccctc aagacgctgt gccattttta aaagctttgc caattgaaaa
    27661 atttcatggg gtagggaaga aaactgtacc tagaatgcat gagctaggga tttatacagg
    27721 aaaagaccta tatgaatgca cagaaatgat gttgattcga aattttggaa aaatgggcta
    27781 ttctctttat cgtaaagtca gaggaatcca tgattctcca gtaaacgtta caagagagcg
    27841 aaaatctgtg ggaaaagagc atacttatgg gcagccgttg caaacggaag aagcagtgct
    27901 gactcagttg cgccaactag ctgaaaaagt agaagaagca ctgagaagag tccaaaaaca
    27961 tgggaaaaca gtagttttga aggtcaggta cacggactat tctactgtga ccaaaagagt
    28021 gacattgcca gaatatatct ataaaaaaga agcactcttt tatcaagcaa gccttatatg
    28081 ggaggaaatc ttgggagtag aaaaaggaat ccgattgtta gggattactt tgacgaatct
    28141 ggacccgatg acttatgaaa acatcgtcct tccattatgg gaaaatcaag aaatataaaa
    28201 acagctttga gagttgaaac cggaaatccg ttttctctcc caaagctgtt tttatatcaa
    28261 gtcttgcttt ttgatttgtt cataggtttc tgttgcatcc gtacattttt cccaaatcac
    28321 aacttgattg gattctaaag atttttggac gtcaatgatc cgctggttgc tacttccgcg
    28381 aaattgtagt tttaggtcac gtttgcttaa ttcaaagcgt ccgtcaacaa ggatatcgat
    28441 ttgcgaaagg agttctaatt tatcttctga atcttgtagc aattcatcaa aggcatagcc
    28501 tgtccatgac cagatatctt ttgtcgtacc gaactctttt cggatacgtc gtacgacttg
    28561 cagacaaaca tctgtattca aaaaaggttc gccaccaagt aaagttaatc cttgcacgta
    28621 atcgttgcgc aagtcagcga tgatttgttc ttctaattca ggtgtgaatg gtttgccata
    28681 tcggaaattt tgtgcagctt tgttgaagca gccttcgcag gcaaataagc agccactaac
    28741 gtaaagactg ttgcggacac cttcaccatc gacaaaatta aacggtttgt agtcagcaat
    28801 atagttttgg ctgtagtctt ttgctaacca ttcttttggt tttggatttc tcataaaaaa
    28861 acttctttca tttaaggttc aaagatatag ctattgaaga taaaatctac tcgaccacat
    28921 aaataagaac aaactgacct aaatccattg ttcaagaaag cttgttctta ttttgataga
    28981 gggcgttcaa agatacaaag ccatcgaaag agggtggtga aaaaagactt gtcatagcct
    29041 cttattctga ataaacggtg ttcaaaagct tgcatctgca gtaataagcc caactaaacg
    29101 aaaaatatga ggaactattt ttgtttagct attcttatta cttgatgcag gacagctttt
    29161 tcacaacctc atttcatgtg tttcacacgt gaggaaattt ctttatggcg gccatgtacc
    29221 atcggacgag cttgcggatt tcctaaatag ccacaagtac gtttgactac atcacatgtt
    29281 tttggatcat gatttccaca ttctggacat tcgaaccctc gttctgtcgg tttgaaatca
    29341 ccttcgaaac cacattgata gcaatgatcg attggcgtat tcgtaccaag atacccgatt
    29401 ttgtcatatg caaaatccca tacagcttct aaagctttcg gattttgttg gagcataggg
    29461 tattcacagt agtggatgaa tccacctgaa caatattttg gataatcctt ctcaaattcc
    29521 aatttttcaa atggcgttgg gtttttacgc acatcatagt ggaaactgtt tgtataatat
    29581 tctttatctg tgatattttc gatgatgccg aatttttctg tatccaaacg gcagaaacgg
    29641 tcagttagac tttcacttgg agttgaatag acactgaaat gataaccata ttcatccccc
    29701 catgcatctg cattagcttt taattctttg acgatatcaa gtgtgaaagc ttttgcttct
    29761 gggtttgttt cccattcgcc accgaagaaa gctgaagcaa cttcatacaa tccaatgtag
    29821 cctaacgata cagtagctcg acggtttttg aataattcat tgacagaatc ggtacgtgaa
    29881 agacgtttcc cgaaagaacc atacatatat aaaattggcg catttgctgg aatggcttct
    29941 ttacaacgtt ctacacgata gagaagcgca tctttcatga tttctaagcg gtcagacagt
    30001 aactgccaga atttattctg atctcctttt gattcgatcg cgattcttgg caaattcaat
    30061 gtaacaacac ctaaattcat tcgtccgaca tttacttctt gaccattttc gtctttccac
    30121 ccttgcaaga atgaacgaca gcccataggt actttgaaac ttccagttaa ttcaacgatt
    30181 ttatcgtaat tcagaatatc aggatacatt cttttcgttg cacattccaa tgctagttgt
    30241 ttgatgtcgt aattaggatc ttcagggttt aagttcacac cttttttcaa agtgaagatc
    30301 agtttaggga agatagctgt ccgcttttca ctccctaatc cattgattcg aatctgtaaa
    30361 attgcttttt gaatttctct ttcaaaccag ttttctccta gtccaaatcc taatgaagtg
    30421 aaaggagttt gtccatttga agtaaataag gtattgattt catattccaa actttgcata
    30481 gcatcgaaga tatctttttt ggtttttgct tttgcatatt ctttttggcg ttctggcgaa
    30541 tcgatccatt cttccgcgtc ttttagatgt tttttatagt tcaattctgc aaaaggagca
    30601 agcaattcat ctgttcggtc ggcagaacag ccaccatatt gacttgacgc tacattggca
    30661 ataatctgtg agatttgtgc tgtggcggtc tggatagatt ttggcgattc gacttcggca
    30721 ttgccgattt tgaacccatt gttcagcatg cctttgaaat caatcaagca gcaattcgtc
    30781 atcggtgtat aaggatgata atccaaatca tggtaatgga tatcgccttt ttgatgcgca
    30841 tttgctatat gtggtggcaa cattttcagt ccgattgatt ttccaacgat cccggctgtc
    30901 agatctcgct gtgtattgaa aacattactg tctttatttg cgttttcgtt cacgacagtt
    30961 tggtccttgt tgattagctt ttcaatcgaa aagttgatat ctgttgcttt actgcgagca
    31021 aaatctcgtt tggtacgata attgatgtac tcttctgcta attcatattc gtttttttct
    31081 agtaatatat gttccacgat gttttgaatc tcatagattt tgacattttc agcaaaccgg
    31141 cttttgattt cttggacaac caagtctgca atcatttgaa ttttttcata agtaagtggt
    31201 gataatgagc cgtgaatttt cttttctgct ttgatcagtg cttcattgat cttttgttca
    31261 tcgaaatcaa catggcggcc atcgcgtttg atgactttca tctgggatgt ctctggctgc
    31321 tgagtaacga cagttttttc ttcataattt actttcatta tcttcaactc ctgctaaatt
    31381 gttcttgtct atcataaaac aaaatcgggg aaggagcaac taaatatcgg ctatatatag
    31441 tattcaatgt gctgtgtaat tggatttcta tactatatgt agtgtatatt ttattgtgaa
    31501 aaaaggtgaa aacttgtagc tggtggattt gttttttgca tatttaattt tgctgaataa
    31561 aataattgaa taaaattttt tattcagtcc aagtaaaagt gacaaaaaca taagagcaaa
    31621 ttattcgtag atatgctact atgcaaataa atagaaatga aaaatgaaag aggaggagaa
    31681 gaattggaaa agatgatcga agtgaaacat ctaagtaaaa gctacggcag caaactttct
    31741 cccgtcaaag ccttagataa tatctcattc acagtagaag aaggcgagtt tgttgggatt
    31801 atgggcccaa gtggtgcagg aaagacaacg ctgatgaata tcttgtccac gatcaatttg
    31861 cctacgatgg gcagtgtctc gatccaagga aaagaaatca caaaaatgaa aaacagtgag
    31921 ctcagtgact ttcgtcgaaa aaaactagga ttcatttttc aagagttcaa tttgatcgat
    31981 accctaaatg caaaagacaa tatcctattg cccctagcag ttgaacggat gactaaagag
    32041 gaaatggaaa agagagtaag acatgtagct caattgttga atatagaaga actgctcaaa
    32101 cggtatcccg atgaactttc tgtcggacag agacaacgaa ttgcagctgc tagagcactt
    32161 gttgttcagc cacaagtgat ttttgcagat gaaccaactg gctctttgga ctcgaagtcg
    32221 gctacagaat tgttgaacta cttgaagacc atgaatcaaa aagaaaaagc aacgatactg
    32281 ttagtcaccc atgatccata tacagcaagc tactgcgata gaattttatt tattaaagat
    32341 ggtgtgattt tttctgaagt tgtcagaaga gggacaagaa gagagttctt tgagaaagta
    32401 atagacatgc aggcgacgat tggcggagga ggaaaaatga atgctgtata aactagccgt
    32461 taaaagtttt tttcggcagt cacgaggcta tcttgtttat tttttcagtt tgactttatc
    32521 cgtaatgatc tattattcct tcagcgcaat gacctacgat caatctttag tcagaagagc
    32581 aagtcaagat acttcgatag atggtggatt gagttttggt ggatttatca ttactgttgt
    32641 tttgcttttt ttcgtattca gtgccaaccg atttttcttg aatagaaggc aaaaggaaat
    32701 aggcatctat cagctttttg gtatgaataa actgcagata tcaggtatat atgtgttaga
    32761 aatcatgata attggattgt tcgcctgtat ttctgggata ttactcggta ttattttttc
    32821 caaactcttt tcaatgattt tggttcgaat gatggatatg gatcttacta gtccattttt
    32881 tatctccatt ccatcagtag tagatacttt atttgctttt ttcatcatcc ttttagcagt
    32941 atctgtatat tcaatatgga aaatatggcg ctatccaatg attcgaagtt ttggagaaaa
    33001 agaacaagca gatagttcaa caatgcgctt caggacaagg caccgattac tcgcagtcat
    33061 cgggttacta cttttaacga gcggatattt tggagctagt cattttaggg aaatcacttc
    33121 atggatgatc agttatcatt atcttgatac attagttttc ttttttcctt ttttaatttt
    33181 tttcgtttgt gtggtcggta cttatttgtt tttctgttat acatttcggt tatttttgat
    33241 catattgagt aaatggaaga tttattacta tggtatcaat tttttgataa tgggtaacac
    33301 gcaagttcat ttgctcaaaa gttggcgtac aaattctctg attacagtca ttctcggaat
    33361 ttctttaatg acaattggcg gcacggctag tatctctact attttatcct accaggagaa
    33421 agtttcctcc ccgatggact atcagtttga tgttgagaca gagaaaaaaa tcaagccgat
    33481 tctagcagag gaagggcaga agataactaa agaaatcacc ttgcattata aagtcattgg
    33541 gagtatctat agtcaaaatc tcgaagaacc tatgtttcaa actgctaatc tgatcacgga
    33601 aacacaatat caagccttca gaaaagttaa tccagatttg ccgaacattt ctttgaaatc
    33661 acctggtcat actgttttgc ttgatcaaat gcaaactctt tttcggaata tttcattata
    33721 tggatctagt attggactgc cagataatca agaattgaag attcaacaaa tgcttccaag
    33781 tgtattaggt gacgaaacaa tggtatatct aagtcctgta ctgattgtag atgataccgt
    33841 ttttgaaaaa gcaaaaggtg tggattatca agtagtcaat gtagatgtat caggtggaaa
    33901 taatgaaaaa atagatgaac gactgagcaa tgacctgtcg ataaagtggc tcaatgctgt
    33961 ttattattct tatgatatcg taaatggaag tctacaagga gaaattagca caaagaaact
    34021 tccggatgac caaatgaagg aagcacaatt tagccaagaa agctctcgtt tgaatttctc
    34081 ttccagctat tcgaaattgc gtgcagttaa tcggcgaatc ggtatcaata tttttgtcac
    34141 actatttgtc gggatgatcg tgattattgc tactggtagt attttgacag tccggcaatt
    34201 agcagaagca gaggaagaaa aagaaaatta tcacctattg actaagctgg ggattccaca
    34261 aagaaggatc agacggatga tcttcgagca aaacgcactt accttctttc cacccatgat
    34321 attagggatc actcacgcag tatttgcaat caatgtcttc actcagtata taaaaactgc
    34381 cgattactgg ttggcttatc ttgtgagtgg cttattgatc cttatctatc ttttgcttta
    34441 tgtcattacg agtcagttat attgtcggat tatcgaggaa tgatatacgg aataagacaa
    34501 gataataaat agaactaaat tttgaatacc atttatttgg aataagaggt tgtgacacga
    34561 tttttgtcac aacctcttta ttttttatat tttttaaaag tacgagaaat tataaagaaa
    34621 aaagcagtga tttgatgtgc tatttttata aaagctatgt gacaaaactg ttagtaaaaa
    34681 aacggtttct gtaagctaga cacatgtttc tttttgtcaa aattagttac actttgtgtg
    34741 aggttatatg aggagggaat ccaatgaaca aatggcgttc taatccaaaa ttgaattatt
    34801 ggggaaaatt catcattcga acaatttttt atagtgcagt actgctgttc ctgatttatc
    34861 tgtatcacta taaaaatatt caaggaggaa cttttatcta taacgaattt taggaggaag
    34921 agtatggaaa tcaaaacgat tattgaagca atcgatgatt ggggaataaa tgaacctgat
    34981 cgagttgctt atcaggcgga tgagacgcat acttttggtg aattgaaaca agcatcagac
    35041 gcacttgctt attatttaga aaaaaaggta gaaggcgatg gaccaattgt cgtttttggc
    35101 aatttggagt ttgaaatgat tgtctcattt ctaggggttg tcaaatctgg ccatgcctat
    35161 ttgccgattg aagagcatac acccaaggag cgtatcctat cgatctttcg ggtggctaaa
    35221 ccatctatgg tcatcagtat cggggactgg atagaagata ttccttcggt tcctgtcata
    35281 acaaaaaaag aatttcaaga aatcaagaag ataccagtag gattttatcc cagaaatagc
    35341 gtaaaaggta cagaaaattt ttatattatt tttacttctg gtactactgg agaaccaaaa
    35401 ggcgtacaaa tcagtcatga taatctagtc agctttgttc aatggacgat ggaagacttt
    35461 gggatcaagc ctggtatgca tttcttagcc caagcgccat tttcttttga tttatcggta
    35521 ttttctattt atcctgcatt agtttctggc ggaatgctga agccgttaaa taaagcagtt
    35581 gttcaagact ttcgtcaatt attcgcgaca ttgccagcct tgaagttgaa tgtgtgggtt
    35641 tctactccat catttatgga tatttgcttg atggaaccta cattcaatgg agaaaatgtg
    35701 ccggaattgg acatgttttt attttgcgga gaagaattga cgaaaaaaac tgcagaaagt
    35761 ctacttgatc gattcccaaa tgcacggata tataatactt acggtccaac agaagcaact
    35821 gtagctattt caagtattca gattgaccag catgtactag atagttatga ccgactaccg
    35881 attggctacg taaaaaaaga cacgaaagtt tctattgtcc aagaaggtaa acctgtttca
    35941 aaaggagaaa cgggtgaaat cattattgca ggaccaagcg tatcaaaagg ttacctgaac
    36001 aatcccgaaa agacagctgc tgcgttcttt tgtcaaaatg acttagcaag ttaccataca
    36061 ggggatgcag gtagactaga tgaatatggg cttttatttt atgaaggacg tatggatcag
    36121 caggtcaaat tacatggata ccggattgaa ctcggggata tcgaacatta tctgctccaa
    36181 gacaaccgaa tcaaacaagc tgtcgtcgta ccaaaatacc aaggaacaaa agtacagcag
    36241 ctggttgctt ttgtcgtatt agaaaacaaa acgaaacagc cggattttca gttgactaaa
    36301 tctatcaaag aacaattact agaagttgta atggattata tgattcctca gaagttccat
    36361 tatgtcgatc tgctgcctca gacagttaat ggaaaaatcg atcgaaaaaa attaacagct
    36421 gaggtgaatc ctgaatgatg ttacctcata tgatccctta tgcagagccg ttttattttt
    36481 ttcttttagc cattgcgctt gtaccgatcg ttctatcttt attgatatgg gaaaaaagat
    36541 taccagttta tcaatcagca gtgacacttt tctttttatt tgtcagcttt ggaggtaatt
    36601 attggcaaca gggagcggcg ctgattgggt atgttttgtg gcagacgata cttgtatggg
    36661 gctattttgt ttatcggcaa aggaaaaatc agacaggatg gttttttgcc gccgttattt
    36721 tagcgataat tccactgttt ttaacgaaag tgactccttt ttttgaacat ggaaaagcat
    36781 cgttacttgg ttttttagga atctcttatt taacctttaa atctgtacaa atgatcatgg
    36841 aaataagaga tggcatgatc aaaacatata agccatttcg ttatatccag tttctgctgt
    36901 tctttccaac aatctcttct ggaccaatcg atcgctatag gagatttgaa aaagatcagc
    36961 tccatccgcc tgttcgtgat aaatatgttg atcttttggg aaaaggcgtc caaaatattt
    37021 ttgtcggttt tttatataaa ttcattatcg gttattattt tggtcaagta ctgcttcctg
    37081 tagtgggacg cattgcctta cagcatggtg gtatttcatg ggcattagta ggttatatgt
    37141 acgtgtacag tatgtatttg ttttttgatt ttgctggcta tagcttattt gcagtcggta
    37201 caagctacat gatgggatat cagacaccaa tcaatttcaa taaacctttt ttatcatgga
    37261 atatcaaaga attctggaat cgttggcata tgacgctgtc cttttggttc cgtgattatg
    37321 tatatatgcg attgatgttc ttcatgatga agaaaaaatt ttttaaaagc aagatcgtaa
    37381 tgtcaaatat cggttatttt gctttgtttc ttctaatggg tatatggcat ggattgacgt
    37441 ggtactacat cgtatatgga ctgtatcatg cttgtctgat ctgtctgaca gatgcatgga
    37501 tccgatttaa gaagaagcat aaagagcagt tgccttctaa caaatttacg catggcttag
    37561 ccgtattttt gacatttcaa gcagtatgtt ttagcttcct gattttttca ggatttttag
    37621 ataaactttt ttttagctaa cgaatagtga catattaatt tatataacaa aggagaaaag
    37681 gaaaatggaa gaacaaattt tatcaatctt agaagaaata acaggaacag atgaagtaag
    37741 aaaaaatcgt gatattgatc tatttgatga agggttgctt gattctttag catctgttca
    37801 attattagtc gagctagacg gagagctgga tatccaagtc cctgtatctg aattcgagcg
    37861 agaggattgg agcacaccga ataaaatcat tcaacaagca actgcattga agggttgagt
    37921 atgaaaaaga aactttttgc tattttcggc ccggttgttt tagctgttgg cttactgttt
    37981 gtgtttttta cctcctccta tcaagtagat atctctaatc cagaaacgat tagacgtgcc
    38041 tcttcttcca tgtcagtgaa cgtgttgaaa ggagatgcga taaaaaatta tgcattatct
    38101 gaaaaacaat atattccttt ttttggctca agtgaattaa gcagaatcag tccatttcat
    38161 ccgtctgttc tggcagaaaa ataccagcga aactatcgtc catttttgct gggagcaccc
    38221 gggacacaat cgttaagcca atatatgatg atgcgttctg caggagatgc tatgaaaaac
    38281 aagaaagtcg tctttattat ttctcctcag tggttcgtta aaaatggtgt aaaaactgat
    38341 tactttaata cgtattattc tgaacttcaa acatacgact ggttgttttc aatgaagaaa
    38401 gtaacgcctg ctgatcgcta tttagctaga agattattaa ctttctcaaa agtaaaagag
    38461 aatgatacgt tgacggcgat tctgcaaacg atcaaaaaag ggaagctacc tttacctgaa
    38521 tctttgaatc agctgcgcag ccaatggaac atgctgaaga gagaggatga agtattcagc
    38581 aatattgggt tgaaagatcg acagcaaaaa atcgatcatg agtcaaaacg attaccaaaa
    38641 caataccaag aaacagaatt atccatttta gcaaatcaaa ttggagaacg agaaacgacg
    38701 aataatccat tcgggttgaa aaacgatttt tatacgcatc gtatccgagc ccacgagcca
    38761 gagctcaaac agtcacaaaa aaattgggat tatcgttttt cgccagaatt ttctgatttt
    38821 cagctggtct tggatcaatt agcaaaaaac cataacgaag tattgttcat catcccaccg
    38881 gttaacgaga aatggagcga ttataccgga ctatctcaag aaatgttaca aggatttgcc
    38941 aaaaagatca aatttcaatt aaatagccaa ggattcaatc gaatagctga ttttgtcaat
    39001 caagcgggta ccaactattt tatggaagac acgatccatc taggctggaa aggctggttg
    39061 gctgcagatc aacagatcag accgttttta gaagaaaatc atattactgc tagtaagtat
    39121 catcttgacg atgctttttt tagcaaatct tggcaacatc aaataccaga taaactacaa
    39181 ttaaaataaa gaaaacaggc gagtgttgct cgcttgtttt ctttattttt gaatattctg
    39241 aataaaagat aaaaatacca agtttgcctg aacaaaaatc ataagcaatg gtataatcac
    39301 aatatgaata tagaaatggg ggaaaacatt tggatacaaa agaagaacag ctacttattg
    39361 atttaacttc tgcaaaagga gtacctggaa atgaagaaga ggtaagagaa gtattcagag
    39421 aatatgccaa accattcgct gatgatattt tttatgacgg tttaggcagt gtgattgcca
    39481 aacatggtgc tggcggaggc cccaaggtct ttatttctgg acatatggac gaagttggtt
    39541 ttatggtaac aaaaataacc gaaaaaggat tcttggaatt ccaaactctt ggaggttggt
    39601 gggggcaagt catgcttgct cagcaagttg aaatcaaaac acgtgaaggg aaaatcgtcc
    39661 acggtgtgat tggttccaaa ccaccacatg ttttaacacc tcaagtaaga aataaacctt
    39721 atgaaataaa ggatatgttc attgatattg gcgcatcaag ccaagaagaa gccaaagagt
    39781 ggggcatacg tcctggagat atggtcactc catatattga atacaaacga atgaatggca
    39841 gtaaatattt actggcaaaa gcatgggata atcgtatcgg gacagctgtt tctctgcgag
    39901 tattagagaa tctatctaaa gaagcacacc caaatgtttt attcgcagga agcgatgtgc
    39961 aagaagaagt aggcttgcga ggtgctcgaa caagtacaca ccttgtaaat cccgatattg
    40021 cttttgcttt agatactggt acagcaggag acactccagg aatgacacca aaagaagcag
    40081 attcgatttt aggaaaaggc ccacagattt tgatttttga tgcatctatg atcccgcata
    40141 aaaaattatt gaattttgtt attgatgtag cagaggaaat ggatattcca ttccaatata
    40201 cagtgatttc tggcggagga actgatgcag gacaaatgca tttgacaaga gatggtgttc
    40261 cgtctgtagc cattaccgtt cctgttcgtt atctgcattc ccatacatca gtgattcatg
    40321 aagatgatta tttgaataca gtgaaactag tcacagaatt agtgaaacgg cttgatagtg
    40381 aaacagttga aaaaattcgg agttatgaat aatgaataat agaataccta tttctgaata
    40441 tcggaaaaaa gcacgagttt ctttggaaaa tcaatgggga atcaatactt ggcttgtttt
    40501 cttatctata atatttacga gcgtcatcca gaactttttt gacggtttca gtccttttcc
    40561 ggaagaaagc atgcagcagc acttattgag ttttctagtc gataatttac ttttattcgc
    40621 attcaactat gcattgtttt acattgcgct agtagttgta cggggtggca gaccaaaaag
    40681 taacctttta ttttcagttt tccaaagaaa gtattttggt tcgatacttg gcatcaattt
    40741 gatcaattca gtcatcaact ggctactcaa cagtttaatc tttctgccca tgtttttaat
    40801 cagcggagtg agtacttata tgcagctgct tttcagttca aatgcggtac ctacgggtgt
    40861 catgccagaa gattttcttg atatcagctt tgttttatcg atgataggga tgatttttgt
    40921 ttcagcactc atcatgagta ttgttggcgg tttattccaa tttgctgcat ggacaaagtt
    40981 cgactatcct gatttgtcgg ttattcaatg tttgaaatat ggctggtttt tattgaaaga
    41041 tagaattggt acgtatattt tgttgcagct gtcttttatc ggctggtatt tgctaggagc
    41101 aatcgctttg ttcatcggtc ttttgtgggt cattgtttat gtgaatgtgt cagttgcaga
    41161 attttatgaa caagcacgga ttgaaaaagg aggtccagac gcctatttta cttagcatgt
    41221 aagcagttta ttcatcaaaa aaataacttt tgtggtagga taaatctttg agggggaatg
    41281 tacgatgaaa ttaactgttt taggatgctt aggagcgtat ccttataaaa atcaagggac
    41341 aacaggttat ttgcttcaat ctgacgggtt caatttgttg atcgatgcag gtagtgctac
    41401 gctgatcaaa ttacaagaac accttgatcc acttgatcta gatgctgtga tccttagcca
    41461 ttatcatcat gatcatattg ctgaccttgg tgtattacag tattactggc aacttcatcc
    41521 taagagaaat ccggagacat tgctttcaat ttatggacac acaaaagatt ccattcactt
    41581 tgaagaattg acgttggaaa atgtctcaaa aggacatcct tattttgaag ctgaagaatt
    41641 aaaaattggt ccttttctga ttacttttat ggaaacaatc catccagtag tatgttacgc
    41701 catgcgtatc gtagaagaaa caacggggaa agtattcgta tttacaggtg attcaggtta
    41761 tttggaaagt ttcgctgatt ttgcgaaaga tgctgatttg tttctagcag atacctatct
    41821 ttttgaagga aatgaacatc atcatgccca tttcacttca aaagaagctg gagaacttgc
    41881 gaaatcagct aacgtaaaga aacttgtact tacacaccta cctcagtttg gtgatttaga
    41941 acaattgaaa aatgaagcgc aaaaggcagc aggcaaaggg attgatatag aacttgctgc
    42001 agttgacaaa gtgtttaaca tttaggagga agaacatgat ttttgtacca aatgaaaacc
    42061 acgacccaag aatcaattta gcaattgaac aatatttgct gcaagaaatg cctttagatg
    42121 aacctatttt gctcttttat atcaatgaac cttcaatcat catcggcaga aaccaaaata
    42181 cgcttgaaga aatcaatcga gattatgtgg aagaaaatgg cattcatgtt gtccgtcgct
    42241 taagtggagg cggagctgtc taccatgatt ttggaaacct gaactttagt tttatcatgc
    42301 ctgatgatgg ggattcattc cgtgactttg cgaaagtaac acagccaatc atccaagcac
    42361 ttcatgaatt gggtgttgct ggggcggaat tgaaaggccg aaatgatcta gtcattgatg
    42421 gcatgaaatt ttctggcaac gcgatgtatg ctaccaacgg tcggatgttt gctcatggaa
    42481 caattatgtt cgactctgat atcaatgaag tagtcaatgc attaaaagta aaaaaagaca
    42541 aaatcgaatc aaaaggaatc aaatcgatcc gttctcgtgt aacgaatatc aaaccatttc
    42601 ttccagaaga aaagcaaaaa atgacaacag aagaatttcg tcaagaaatc ttgttgaaaa
    42661 ttttcggcgt agagtcagta gaagaagtaa aaacgtacga attgactgat gaagattgga
    42721 aaaaaatcaa tcgaatctct gaggaatatt atcgaaattg ggattggaat tacgggaagt
    42781 ctccagattt caatttctct cgccaaaaac gtttttctat cggttcgatt gaagtccatt
    42841 tgaatgtctc agaaggaatg atcaaagaag caaaaatctt tggcgatttc ttcggactag
    42901 gagatatctc tgacgtggaa aaaaaattgg ttggacagaa atatgataaa gcatctctat
    42961 caaaaatagt cagccaaatt gatgtgaaga aatattttgg tgcgatagaa ccagaagaat
    43021 tgcttacctt actttattga aaataaaaaa tagcggaatc cacatatatg gattccgcta
    43081 ttttttatac aggattcggg taatttcgag agagttgaac agtgatccga tcaatgaaag
    43141 tatctttttc ctgcgttagt gttcctgtgt aacgatccgc tacgtctttc atcatcatta
    43201 agtcaaaacg ttcctgatca tctaattctt ctgtgatgcc tcgactaaat aggtttgtat
    43261 cgtaatgtgc actatgttcg caacaaatga cgaaatgttc ctcttgttcg tgtatcgaca
    43321 aataaatttt tcgatacagt ggattttcga tggcatgagt ttctctaaag gaatgctcta
    43381 aagcgtggat caaaagttga gccagatcgt catcgttgat aggaagttct tcgggaagat
    43441 ctgcttggaa ggttatctga atattccgtt tggcagctaa cttctggtaa cgcgctaaga
    43501 taagatttgt cacctggtgg ttgcaaaaag aacacagcat ttcgaaattt tttgcttctt
    43561 ctaatatctt ttccacatag tttttagctt ttgtttggtc cgtatgcatt aaatcttgca
    43621 gattctccat ctcttggaca aattccaggc gaagtgtatt tacttcttgg atatgctgtc
    43681 gaagctgctc ataatgttgc tccaacaaat catttttgat ttttaagaaa tctacttgtc
    43741 tccgatcttt gtcggtattt aagatgtgat cgattagatt gtagctgata ataacgatta
    43801 gaatgataat atataaaatc gtagaccaat tgatcgttgt ttgatagata cctaatgccg
    43861 tttggatgta aatcaagcaa tggataatgg ctaccaagac gatccaaggt gtacaagcta
    43921 cataaaaacg gttattctta tatgcttcgc caatgcatgc aagtgaagtg actaaagtgc
    43981 ttataacact agcaatatta gcaatcggca ttaattcagg aagttctgca ggagtaaaag
    44041 ttgcataaag cagaacgccg ataaaaatca aaagttggaa cgtacaaaaa attccgtacc
    44101 attttttata gtaaaacatt cggctccaat agtagcagat gatgaaagct gacgttaaga
    44161 taataaataa atgagataaa aaagaatgaa cgaatggttc aaaaagaata ccacttaaaa
    44221 tatcttctga tacagcttca aatcctaatg ccaaagcaaa gcaacttaag agaataagtg
    44281 agtattcaag acgtttttgt ttatacatca agtagatcgt atagaaaaag gtccctatag
    44341 cgataaaaac agcgattatc atcgtcaata cttgcggcac tgattgtgaa taaataaaac
    44401 taatcaatgg tccagttgtt cctagataaa ctttcggtgg cagtccagca tagttctgat
    44461 aaggagatga gactgtaagg gtcaattttt ttccgatata atttgctggt aaatctatca
    44521 cagccaatga gagtccggga ttgctttctt cctgataatc tttccgttgg tataaaatct
    44581 tttcgcctag atatacagta atccattgat ggtttccttt gattagtaaa tttgcttcgt
    44641 ccaacggttt ttcaagtgtc gtttccataa tgaatgtttc gtctttatcc acggtaggaa
    44701 tcgtacgagt gtacttgctg tcaaacttaa aatcaggatc atcttttgta tagaaaaccc
    44761 acgaatcttt tatttctttc atttgattgc tgtccgcagg agaaaaaata aaataaaaga
    44821 aataagcaat tcctgttaaa aataacaatg ataatataat gattatgctc cactttatac
    44881 gtaaataaaa agattctttt ttattatata tgttattaaa aaaagacata aaggtattcc
    44941 tttctaaaac ttgtaatgtt agggtaaacg ttttttaaac taacgtcaat tatttataat
    45001 aaaaaatgag ataaagatga ggaaatagaa ttagaaacat ataattatta aagggaaaca
    45061 tttttacagc tataatttga aaaaaattta tcgttattat atgattaaga aggattgaat
    45121 aaagaaagaa gttgaaaacg atacctgatt acaaaaaaat aacttttata agttctataa
    45181 cgttgattat atttttaatt cttattcttc tcgtcgtctt ggttccccga atttttggtt
    45241 tgactcctta tattattgag gacaaagaaa tgagtccata ttatgcacaa ggaaatctga
    45301 tatatgtacg accgaaaaat acaaatgaga ttttagtcgg agatattatt acatattatg
    45361 aaaattcagg aaaaaaaatc tcgacgcgtc gcgtaatagc cgttgaaaac aaccacgaag
    45421 atttttatac aaaagctgat acaaagactt atattgagcc tggagtagta aaaaagagaa
    45481 atattatcgg tagtcccatc tttcagattc catatattgg actagtcctt tcaaaaacgg
    45541 catttgcatg gattcagggg ttattcttta cgattgcttt ttgcctaaca ggaatcacag
    45601 catggaatac ctttgtagag ttgaaaaaga agcggagacg aacacagaaa tttttgtaag
    45661 aatgttaaaa acataaatgt attccagaga tccaacgact cttctgaaca tgaaacacga
    45721 gaaaacactc atgcagcttg tagatttagt cagttgttcg tataatgtgt gaagaggagg
    45781 agtagtatgc gcaatgaaat gatgcatcgt cctgtagtca aagaagaact attggatttt
    45841 atgcgtaccc agcaaaaaaa attaccgggt gagctaggca aactggaaga agaagctcat
    45901 gtagcagaag taccagttat tccccatgaa acagttgtat ttttgaaatt tttacttgga
    45961 cagcttcagc ctgaacgaat cttggagatc ggagcagcta tcggtttttc atcaagtgtc
    46021 atggccacga caatcggaga aaacgctcat gttacgacaa ttgatcgttt cgatgtcatg
    46081 atcgagaaag ctaaaaaaaa ctatgaacgt ctaggactga cagataaagt gactttgctt
    46141 gaaggtcaag cagcagatat cttgccggag ttgtctggtc cgtatgactt tatttttatg
    46201 gatagtgcaa aatcaaaata tattgaattt ttacctgaat gcttacgttt gttaagaaaa
    46261 ggtggcgttt tgatggtaga tgacatcttc caaggaggaa cgatcttgct cccagatgaa
    46321 gagattcccc gtggcaaacg agctatccat cgtaaattaa atgaatttct tcgtgttgtg
    46381 atggatcatc ccgatttgac atcgacaatc ttgccattag gtgatggtgt gatcttaatg
    46441 acaaaggaat cagaaacaat cgagttgtga tacatgatta tggcagcctg tagataatcg
    46501 attgaatcga gggctcaatt cgaacttgat gtaaaagttt ctatatttgt aagtcaatta
    46561 aaggatacag gaaatctaaa taaacaggag tagcatgaag tcaatgtagc tcctgttttc
    46621 aattgataac cttagaacag ctgaaggaga atgtctcgtt ttaaaagatt gatttagtag
    46681 ttgggccaaa ataattcatt atttgctaat taaataacta tttcgtattg acagaagcaa
    46741 tttaattatc tataattatt gatgttccgc tgattcttgt catagtagct cagctggata
    46801 gagcatccgc cttctaagcg gacggtcggg ggttcgaatc cctcctgtga cgctaaaaaa
    46861 ccgttagaaa ctgaggtttc taacggtttt tctttgtttt ttgtgccaat attgtttgtt
    46921 ttcaaaggca aataaaataa aaaaacatcc ttatcgagta caattataag gagcaaaaaa
    46981 ataaaactca tcagaaaaga ggaagaagat atgacgatac tatctagaat tttagtgact
    47041 ctagttgcaa ttgaattttt ttatatcatg tatatcgaaa cggtgagaac tgattctgac
    47101 acgacaagta gggtttttaa aatgagtaaa gaagagttaa gcagaaaatc tgttcaaaca
    47161 ttgtttaaga accaaggagt gtacaatgga ctattaggag tgggactact ttatggagct
    47221 tatctttctt ccgcatctaa agaaatcaca agtatgctat taatatctat tttttttgtt
    47281 gcattatatg gcagtttgac tagcgatcgt tggattttac tcaaacaagg tgggctagct
    47341 ctgttggcat tgctttctat tttcttataa atatagaaaa aataagaatt tatctaacag
    47401 gaaactttgt gaaaaatatt actttatctc atgaaatatg atattctaat gaatggtatt
    47461 ttagaaaatt tcatgaaaaa ggagttagat aggtgttcaa aaattatgtt tcctcactaa
    47521 aaaaagaatt ttcaggttat gatctttcaa gatttcagaa agatcttttg gctgggatca
    47581 cagtagctgc tgtagcattg cctttagcct tggcttttgg ggtatcaagc ggagctgatg
    47641 cggccgcagg tttgattacg gctatcattg ccggtatcgt tattggcggt ttatcaggcg
    47701 gtttttatca aatatcaggt ccgacaggtg cgatggcagc tatcttgatg tctattgcgg
    47761 caacaaaggg aatgcaaggt gtattgcttg ctactttttt agcgggtcta tttttacttg
    47821 cagcaggact tttccgatta ggaacactga cttcttttat cccagcaccc gtaattacgg
    47881 ggtttacttc aggtattgct atcattattg cattaggtca gatcgataat ttatttggtg
    47941 ttcactcgga aggtacaaac gttattgaaa aactaagtag ttatcaaacg ataggatttc
    48001 cgataaacac agcctctttg atcatgggag ggctagtcat tttagggatg tttatctatc
    48061 caaaaaaatg ggcgaaacga atgcctagtt cgctgctggc tatcattttg gcaacagcag
    48121 ttatgctatt gttccattta ccagtagcaa ctgtgggtaa gattccacaa acattgatca
    48181 gtagtaatcg tttgaatatg ggagatttta gcttttctgc cttgcaacaa gtggcagttc
    48241 cagctattag cattgcttta ttaggaatga ttgaaagctt gctatgtggt gcttcggctg
    48301 gccgaatggc aaataagccc ttagatagca atcaagaatt aatggcgcaa ggaattggaa
    48361 atctgttgct tcctttcttt ggcgggattc ctgctactgc ggccatcgcc cgtacaagcg
    48421 tggccatcaa atctggtgca caaactagat tagctggtat gatacatgca gttgtcttac
    48481 tgctctccat gcttgttttt gcgccagtga tgtcacagat tccaatgcca gcacttgcag
    48541 gtgttttgat tgtgacggct tggcgtatga atgaatggga aacgatcaag gagctattta
    48601 ctaataaata ttggtcagca gttctattgt ttatccttac gatgatttgt acagttatct
    48661 ttgatttaag tattgctatc gtgatcgggg tgatcggcgg ctgtgtattt tttgttgtca
    48721 aaagcgctgc tatcacgatt tcattagaaa cgatcgattg gcaaagaatg gggctgccag
    48781 aaacggaaaa gctgaataac tggacagtag tgtatattag tggtccactg ttttttatgt
    48841 cttcagaaaa actcaaaaat aaattggaag aactaaatca tcacgatggt gtcattttct
    48901 ccatgcgagg cgtaccaagt attgatttga cggcacgtgc agtttttgat gaatttcaag
    48961 aaaaagcaga gcaaagagga caaacgatta tctatacttc cttgcagcca gaagtagaaa
    49021 aacaactttc tcacttgtgg gaacatacat tcacgcatca acatcaaaca gttgctcatg
    49081 cgttgacagc tttacatgcg aaatatactg caattagcca ttaaaacaat gaaaaagaac
    49141 agacaagact gtaaactgaa gattttatgg tcttgtctgt tctttttaat attgctgtaa
    49201 aagtgaagct agtacttgcc agtcttgatg gataacaggt acaagggagc gattgtagaa
    49261 aatagctgct ggatgaaaaa gtggaagaat tcgatagttt ttttctgacc attcgtaggc
    49321 atccttttgt tctgttaatt gtaagacagg ccctgtatag agttgaccat gatttttggt
    49381 aaccgtatat tctttgccaa gtaaacgttg aagaccaatg tttccaagtg tcgcaattaa
    49441 ttttggctgg accttcatca gttcgtaatc caatattggt gcatgcgcaa atacttcgga
    49501 acgagtaggt gtccgatttg gataaacagt ttctgactgt tgtgtccgtt tattgatccg
    49561 atgagtcacg cgatacgggc ggctccttac agcactggtg atatacacct cttctcgtgt
    49621 taagccagtt gaagaaagtg cctgcatcaa ttcttttcca gcttgaccgc taaaaggaac
    49681 atgcgtatca atctctgttt tccctggggc ttctcccacg agcatcagtt taggatgaag
    49741 aggtccttgc ccagcgataa atccttctac aggacggccg ttcattctta aagaagcttc
    49801 ttgtatcagt tcttttgggt attccattta tgctcctcct ttttctttta ttataagaat
    49861 aaaagaaaca caatgcgact attttatttg gtagaactgt gaggatagaa aagcagaagt
    49921 attgcttgaa tttttgtttt actttttgat tttaaattta gttgactaac ggttgctgtt
    49981 ctggtaagct agtactgttg taattgtgga ctccacagct acaaccgctc agaacaagta
    50041 ataagcacag catatgctgt cacttcggat ggcgagtcta agtatatcag aaggaggtgc
    50101 tttatagcat gtacgcaatt attaaaactg gtggtaaaca agtaaaagtt gaagtgggac
    50161 aagcaatcta tgttgaaaaa cttgacgttg aagcaggaga aaaagttgtt tttgacgaag
    50221 ttatcttagt aggcggagaa tctacaaaag taggcgctcc aactgttgca ggtgcaactg
    50281 ttgaaggtac tgttgaaaaa cacggcaaac aaaagaaagt cgttactttc aaatacaaac
    50341 ctaaaaaaca ttcacaccgt aaacaaggtc atcgtcaacc atatacaaaa gtggttatcg
    50401 acgcgatcaa cgcttaatag cgtttgagaa agaaggccta tatatgatca aaggaacatt
    50461 taagcgaaat gacgcaggtc agatcgtttc attcactttg accgggcatg ctgatgctgg
    50521 tccttatgga agtgatatcg tttgtgcggg tgtttctgcg ttagcgatca gcactgtcaa
    50581 cgggattgcc tcattagccg gttttgagcc gattgttgaa atgaatgaag aagaaggcgg
    50641 ttatctttat accgaagtca cttcaggaat gacacaagaa caaaacaata ttgctgagat
    50701 tctattagaa aatcttttat taggattaca atcgatagaa gctgaaaact cagaatatat
    50761 ccaaattaaa acaattaatg ataaatagga ggtgcagacc atgttgttat ctatgaatct
    50821 acaattattt gcccacaaaa aaggtggcgg ttctacatct aacggccgtg actcagaatc
    50881 aaaacgtcta ggtgctaaac gtgctgatgg acaaacagtt acaggtggat caattcttta
    50941 ccgtcaacgc ggtacaaaaa tctatccagg tgtaaacgtg ggtatcggtg gagacgacac
    51001 tttatttgca aaagttgatg gcgtagtacg tttcgaacgt aaaggccgta acaaaaaaca
    51061 agtgtctgtt tatccagtag ctcaagaagc ttaattgaat cttatgatta aagaggttgt
    51121 gacaagactt ttgtcacagc cttttttgtt tataaaaata aaagttgtag tggaggctac
    51181 gaagtttact ttgtcttttt acacttaata tttttttgcc aatttggaat caggatgttt
    51241 tgataactat cgttttcaat tgatggaagc tatattgttt gctatcagtg aaataattat
    51301 atggtaaaca gtgttctttt tcttgtttcc attataaaaa tcgtatcggg atttttcgtt
    51361 cttttaaatt gaacatttct atctatccgc gtgttatcaa caaaacaaaa tcaggtaaat
    51421 agttgacgaa acagaaataa atacatagta ttatgttttg aagcctaagt acaatagtct
    51481 atagaaaagg agcgatacca ttggcaaaac gcattgaccg aatttatatg tatgtcaaag
    51541 aaaaaaccgc tcatttaaca aaggcagaat atgatcaagg agtaacaaca caggaaattg
    51601 ctgaagtgtt agggattcag cgaacaaata gcagtaaaga tctgaatcag ctagtgcgcg
    51661 aaggaaaact gataaagaca gacggacgac cagtacgtta catatatcaa acactcgtta
    51721 cgcacgtaaa gccagttaca aaacatgtag tcagttacaa agaaacgccg gtctctgaag
    51781 aaaaaaatac actccaaata gatactaagg atatctttgc aaaaatcatt ggagctaacg
    51841 gcagcatgaa aaattcagtg gaacaggcaa aagctgcgat tttgtatccg ccaagaggat
    51901 taaattgttt gattacagga cctaccggat caggaaagac ttattttgca cacgcgatgt
    51961 atcattttgc aaaagcgcat catgtaatag atgaggaaac ggaactgatt gttttcaatt
    52021 gtgctgatta tgccaataac cctgagcttt tgatgagtca tttatttggg tacgcaaaag
    52081 gtgcttttac aggcgcagat gaagaaaaaa tggggattat cgatcaagcg gatggcggta
    52141 tgctgttttt ggatgagatt caccggttgc cgcctgaagg tcaagaaatg atcttttact
    52201 ttatggatca tggaacttac agtcgtcttg gggaaacaac gaaatcccat gaagcaaatg
    52261 tacggatcgt aggtgcgacc acagaagacc caggttcttc tctattagaa acttttgttc
    52321 ggcgtatccc aatcaatatc aaacttccgg cttttgaaaa acgtcctgca aatgagaaag
    52381 tcgatttagt aaaaatcatg attgcccacg aagcaaaccg cacgcagcga aagatttctt
    52441 tgacagaaga tgtagcaaaa gcattgatcg gcagtgtgac ctatggaaat ataggacaat
    52501 tgaagtcaaa tatccagtta gtatgtgcac gaggatttct aaatcatatg caatcacccg
    52561 aaatctcgat cacaatcgat gatttgacag aagggattcg tagcggattg attcaattag
    52621 caagcaaccg cagtgcaatg gcagagcttt ctaaaatatt ggagccaaag ataactgtag
    52681 caccaaatga tacgatcatg aaaatccaat cggactccta tgagttacct tataatctat
    52741 atgatatcat cggagataaa gcagctttac tgaagtcgga tggtttggat caagaagcaa
    52801 tcaatcattt catttctaca gatatcaaca tccatctgaa gtctttctat aaagaccatg
    52861 gtttttcttt taatgcagac agcaaattag cagaatttgt agatccgaaa atcatagaag
    52921 tgaccaatca gatttatgta atggtcaaaa gatctttacc atatgagttt cagcaaaact
    52981 ttatttatgc tatgagcttg catatcagtt cttttttgaa aaaaatcaac ttaggagaag
    53041 aacggcatac gaatgataac atccgtgaga tggcttttga ttttccagat gaatatgctg
    53101 ttgcgaaaga agtgcgtagt tatatcgaaa cgtattttca agtggagatt ccagaaagtg
    53161 aagactatta cttgaccgtt ctactcgtct cgttacgggc aaatcaagca agcggcagaa
    53221 tcggtgtagt cgttgcagct cacggaaaca gcacagcaag cagtatggtc caagttgtcc
    53281 aacagttgtt agatgcagag aacgtacgag cagtagacat gcctttggat atggatccga
    53341 aaacagcatt ggaccgaatt gaacgcaatg tacaagaagt agatgaagga agcggggtca
    53401 tactcttagt agatatgggg tcccttgcgt cgtttaattc gcaaattcaa agagacactg
    53461 gaattccagt acgtactgtt gatatggtaa caacttctct agttttggaa actgtacgaa
    53521 aggtctcagt attagggaca gatctagata tgttgcatga atcattgaaa aattttcgag
    53581 gttacgcaga aatatcttct gtagaatctg ttcaggaagc aaaacctatt cataagaaaa
    53641 aagctgtttt ggctatctgc gcatctggcg aaggtacagc acaaagaatc aaagaattga
    53701 tcgaacgagc ggtaaacaag cgtcaagaaa cagaattaga agttctagct ttgtcgattg
    53761 tggaagtgaa agaagaactg ccagttatcc aaaaagaata tcagatcata gctactactg
    53821 ggattactga tttgaatatc caagccccat ttattccttt agaacgtttt attgatcaaa
    53881 atatcgaagt cattttggat cagttgctga tggaatcaga attggaggaa actgaattca
    53941 tctccctaga tgaagagtca gctaaaaata cgtgtgttga gtttatctct gataacttca
    54001 tctttattaa tggtagtaag ttaatcgatc caatgtggca attttctaca caaatctctc
    54061 agacgacggg aatcggcgat gaagaatatg gattcaaaat caacctagtg atgcacacag
    54121 caggaatgat cgaacgtatt attcggaatg aaccattgac tgtggaagaa aacgaattga
    54181 caaatacaac gaatgatcct ctttatagcc aattggcagc ctcagttgtt ctgttggaag
    54241 atcagataaa agtaaaagtg ccgatagaag aaatgtatta tctgctacgt ctagtccata
    54301 atcagttaga taaaaaagaa tacactgtgc catgaataca caaaaacgat acacttttat
    54361 agtgtatcgt ttttgtgtat tggttaagaa tcctttaaat tcaattagtg tatcgtaaaa
    54421 aagttggcac gctttttgct tatagtagtg tgtgctagga ggtggttgtt cacacagaaa
    54481 tgataggggt ttctgtgaag tagtgaggaa gaaggagttt tgcagatgga tattcgattg
    54541 gcacgtatag atgatcgttt aatccacggg caagtcgcaa cagtttggtc gaagcaaaca
    54601 ggtgttgaac gtatcattgt gatcagcgat gtagtcgctc aagataaatt gagaaagttt
    54661 ctactaaaag aagctgcacc gccggggatc acagcaaatg taataaccgt gcagaaaatg
    54721 attcaaatct atcatagtgg attattatca cgagcaaaag tgatgctttt attcaccaat
    54781 ccccaagatg ttgaagcggt tgtccgctgc ggtgtacagt tgaagtcgtt gaatatcggc
    54841 ggtatgagtt ttactgaagg caaatcaatg attaccaatt ttgtttcagt aaatcaacaa
    54901 gatattgatt cctttcgtta cttgaacagt caagaagtgg agttagagat tcgaaaagta
    54961 cctgctgaca ggaaaatcct tttgatggaa ctactagcga aaatcaaaaa ggtgtagcaa
    55021 gaatggttgt tggaaaaggt caaataattc tgttgtaatg gttagcaatt attaatattt
    55081 ttgaatttgt tacaacaaaa gtaaatagga ggtataaata tggtagggat tatcctagca
    55141 agtcatggac aatttgctga aggaatctta caatccggtt caatgatctt tggcgaacaa
    55201 gaaaacgtca aagccgtgat tttgaaacca agtgaaggtc cagatgactt gagagccaaa
    55261 ttggaagaag cggttgcttc ttttgataat caagacgaag tgttattctt agttgattta
    55321 tggggcggaa caccgttcaa tcaatcaaac acattattcg aagagcacaa agataaatgg
    55381 gcaatcgtca gcggtttgaa tttaccaatg ttgattgaag cctacgcatc acgcttttca
    55441 atggaatctg ctcatgaaat tgcagcacac atcattgaaa cagcaaaaga cggtgtaaaa
    55501 gtaaaaccgg aagaattaga accggcagaa gcaccgaaag cagcagttga agacgcacaa
    55561 cctaaaggag cacttccaga aggaactgtg gtcggagacg gaaaaatcaa atatgtatta
    55621 gcacgtgttg actcacgtct gttacatgga caagttgcga cagcttggac aaaagcagtg
    55681 caaccaaacc gtattatcgt tgtttcagat gcagtatcaa aggatgatct gcgtaagaga
    55741 ctaatcgaac aagctgctcc tccaggtgta aaagcaaatg ttattcctat tagcaaaatg
    55801 atcgaagtag caaaagaccc tcgttttgga aataccaaag cattgctttt atttgaaaat
    55861 cctgaagacg tattgacagc tgttgaaggt ggcgtagaca tcaaagaatt aaatgttggg
    55921 tcaatggcgc attcggtcgg taaagtcgtt gtaagtaaag tattgtctat gggacaagaa
    55981 gacgttgaag cgtttgagaa gcttgaacaa aaaggtgtga aattcgatgt acgtaaagta
    56041 cctaatgatt cacgcgataa catggatgat attttgaaaa aagcaaaagc tgaattagca
    56101 aaagcgtaat aaataatatt aaaatttata ggaggcttat catgtctatt atttcaataa
    56161 ttttagtcgt attagttgcc tttctagctg gtatggaagg tattcttgat gaatttcaat
    56221 ttcatcaacc actagtggca tgtacattga tcggtttagt taccggtaac ctcgaagctg
    56281 gtatcgtact tggcggtacg cttcagatga tcgctttagg ttgggcaaat atcggtgccg
    56341 ctgtagcacc agatgctgca ttagcatcag tagcatcagc aattatttta gttttaggtg
    56401 gacaaggtgt taaaggtgtt ccttctgcga tcgcgattgc cgttcctttg gctgttgcag
    56461 ggttgttctt aacaatgatc gtacgtacaa tcgctgttcc aatcgttcat ttgatggatg
    56521 cggctgctga agaaggaaac atccgtaagg ttgaaatgtg gcacatcatt gctgtatgtt
    56581 tacaaggggt tcgtatcgcg gtcccagcag cagcgttgct atttatccca gcacaaactg
    56641 tgcaatcatt ccttgaatca atgccagcat ggttaacaga tggtatggct atcggtgggg
    56701 gaatggtcgt agctgtaggt tacgctttgg taatcaacat gatggctaca aaagaagtat
    56761 ggccattctt cgttattggt ttcgttgtag ctgcaatttc tcaattaaca ttgattgctc
    56821 taggtgcttt aggtgttgca ttagctctta tttacttgaa cctttctaaa atgggtggtt
    56881 catcaaataa tggaggcgga agcaactctg gtgacccttt aggcgacatt ttgaatgact
    56941 attaatcttg aaggaggaaa ataaaatggc agaagaaaaa atcaaattat caaaaaaaga
    57001 tcgtttagct gttgcgtggc gctctacctt cattcaaggt tcatggaact acgaacgtat
    57061 gcaaaacggt ggctgggcat tttctatgat tccagcaatc aaaaaattat acaaaacaaa
    57121 agaagaacgt tctgcagcat taaaacgtca cttggaattc tttaatacac acccatacat
    57181 tgcttctcca attttagggg ttacattggc tctggaagaa gaacgtgcga atggcgcgcc
    57241 tgttgatgat gtagcgattc aaggggttaa agtaggtatg atgggtcctt tagctggtgt
    57301 tggtgaccca gtgttctggt ttacagtacg tccaatgcta ggtgcactag gtgcttcatt
    57361 agctatggga ggaaatatcc ttggaccaat catcttcttc ttaggttgga acattattcg
    57421 ttgggcattc atgtggtata cacaagaatt tggctacaaa gctggttcta aaattactga
    57481 tgacctttct ggcggattgt tacaagacgt aacaaaaggt gcttcaattt tagggatgtt
    57541 cgtattagct gcccttgtac aaagatgggt atcaatcaaa ttcttaccag ttgtatcaac
    57601 tgtaaaatta gataaaggtg cttacatcga atgggataaa ttgccttcag gtggtgaagg
    57661 aatcaagagt gcatttgaac aagttaataa tggcttagcc ctttctcctg aaaaggtaac
    57721 aacattacaa gacaacttag accaattgat tcctggttta gctgcattag gattaacatt
    57781 gttatgtatg tggttgctta agaaaaaggt ttctccaatc gttatcatcc ttggtttatt
    57841 cgtagtcggt gttgttggac acgtcatcgg acttttataa tccataaaaa tatagctcgg
    57901 gcgttgtccc gagctttttt tatatagtat actgttcata aacaaatatc tggagggaga
    57961 aaatggttca atcaatcaat acaaaagtag acttggtaat tgatgctact tcgtttacgg
    58021 gtctgacaga ttacgggcaa atcatgatag gtgataatgg ttttgagttt taccatgcgc
    58081 gagatagacg caaatatatc caaattccat gggaagaagt ggattatgta ctagcatcag
    58141 tcatgctaaa gggaaaatgg attcctcgat atgccattca aacgaaaaaa aatggtacat
    58201 acacattttc ttccaaagaa cctaaaaaag tgcttcgtgc gatcagagaa tatgtagatg
    58261 aagatagaat ggtacaatca ttgagtttct ttgatgtagt gaaacgtggt attcgtagta
    58321 tgtttaaacg caagtaaaaa ataaagaaaa cttctaagag gaggttttct ttattttttt
    58381 actttgcaga agaaaatatg aaatagcaaa accaattatt tgtccccttt aaatcccata
    58441 tgctaaaatt gattagcaaa gaaggaggtt atattatgag ttggttatgg acattgattg
    58501 ttggagccgt gatcggagca attgcaggtg cgatcaccag taaaggaaat tcgatgggct
    58561 gtgtaacgaa tatcattgct ggactagtcg gttcaagtat tggacaagcg attttcggcg
    58621 aatggggacc aaaagctgca ggtatggcac ttattccatc tattttaggc gcagtgattt
    58681 tggtagcagt tgtctcattt ttctttggaa aacgatcata agtttttaca aaatagtaaa
    58741 ctaaaaggag ttgcagcgag actgtgactc ctttttggct ttatttattt aaaataaaaa
    58801 gaagttttcc ggaaattttt atttaactgt cgtgtacgaa gaaaagtttg ctataatgga
    58861 aaaaagcgtg caaagaaaga gggagtttca ttatgatgct acgtgtagaa aaattgcgca
    58921 aaaaaatgca agaagaaaat ttggattcat ttttggttac cagtccgtac aatcttcgtt
    58981 acttaacgaa ctttacaggt acgacaggat tagctgttat tactttggaa aaagcatttt
    59041 ttattactga ttttcgttat accgagcagg cggcagcgca ggctcaaggg tttgaaatta
    59101 ttaaaaatgt cggacctatt tttgatgagg tagcaaactt agtacgcaaa gaagaactgc
    59161 actcattagc ttttgaggaa acaacagtct catttttaga atattcggtt ttagaagaaa
    59221 tcatcgatgc ggaattagta cctgtttcac agatgatcga agaactcaga gaagtgaaag
    59281 atgaagaaga aatcgctatt atcgaaaaag catgtcatat tgcggatatg gcatatgatc
    59341 acatcctgaa aatgatccaa ccgggcatga ctgagattga agtcgcaaat caactagact
    59401 tttatatgcg ttctcttggc gcttcaggtg tttcttttga tacgatcgtt gctagcggat
    59461 tgcgttcggc tatgccgcat ggtgtagcaa gcaaaaaaat catcgaacaa ggtgacttga
    59521 tcacattgga ctttggttgc tattacgaag ggtacgtttc cgatatgaca cgtacattcg
    59581 ctattggtga tcctggggaa aaattaaaag aaatttatca aatagtctta gatgctcaat
    59641 tagcagttat tgatgctgca aaaccaggaa tgactggtgt ccaattagat gcagttgcta
    59701 gagattatat ttcaaaacat ggttacgggg aagcatttgg acattctaca ggacatggga
    59761 tcggtttaga gatccacgaa ggaccaaacg tttcttctcg tgcagaaaag caatttgtac
    59821 caggaaatgt gatcactgat gaaccaggga tctacttacc aggaatcggt ggagtacgta
    59881 ttgaggatga cttgttgatc acagaagatg gaaatcgtgt gttgactcgt tcaccaaaag
    59941 aattgatcat cttatagttt catcaataga agaagtaagc gtaaaaatca gtgttcacag
    60001 actttgttat agaaaatcac tcgatttaat gatacactat agaagaactt ttaagctgta
    60061 agagataata aagttgggtg ttttctccaa ttcatagcag tttgcagctg atcggcggca
    60121 gttttcttac cacgtgatca ctatgccgca tcatagagaa ttcggatgta tctcgtgctt
    60181 aggcaaaggg aggaataaat aaatggcaga tgaaaaaaat ttgattttaa acgcaaacca
    60241 agatttagga gagatcgtga tcgctcctga agtcatcgaa gtaattatcg ggattgcagc
    60301 ttctaaagta gaaggcgtat acggaatgcg cggaactttt gccagcaatg tcactgaatt
    60361 attaggacgt gcagctcacg gaaaaggtgt ttacttagca aacgaggaag atggcttgaa
    60421 agtagatctt tactgttatc tagaatatgg tgtttcagtt cctaaagtag caatggaaat
    60481 gcaggaacgg gtcagccagc aagtcctttt catgacggat atcgatttat ctgaagtcaa
    60541 tatccatgta gtagctgttg ttcctgaaaa attagaacaa ccaacaatcg aagaactttt
    60601 tgaagacgaa gaggaagaga atgaataaag aattatcaag acacgagatt cgggaaatgg
    60661 cattgcaggc tttatttcca ttagacttca atgcagacct aacaaaagaa gatgcgattt
    60721 tcaacgcaat cgaattagac caccgcgaca tgatcaacga agatgaatca gagtttgttc
    60781 ctgtttatct tgatacgcta gttggcggtg tctgtgcaaa aaaagatgaa ttggataaag
    60841 tgattgaaaa acatttaaaa aacaattggc acatcaatcg gatctcaaaa atggatttgg
    60901 tgatcttgcg tatcgcgatt tttgaaatga cttatgtgac agatgtacca gcggctgttg
    60961 ctttaaatga agcaatcgag ttggcaaaaa catttagtga tgaccgttca cgtaaattcg
    61021 tcaacggtgt tttatccaat gtattgaaag aattagaagc tggggcgtaa gtcctagctt
    61081 ttttctatga atcatcataa acctgttata aatcgggaaa attctatggt agaatgaaaa
    61141 cacaaaaaat atctgggagt ggttagatgg cagaattgat taacggaaaa gaattagctg
    61201 aaaaaatgca ggcagagatt gctgaaaaaa tccaaaaatt gaaagataat ggtatccatc
    61261 ccggattagt cgttttattg gtaggggaaa atccagccag ccaaatctat gtaagaaata
    61321 aagaacgtgt agcaaaagaa atcggaatcc attcattggt agagcgttat cctgagacga
    61381 tttctgaaga cgaactactt gcagaaattg aaaaatacaa tcaagatcct agttttcatg
    61441 gtatattggt ccaattacca ttgccaaagc atatagatga agaaaagatc ctgttagcta
    61501 tccgccctga aaaagacgta gacggttttc atccgatgaa tttaggcaga ctccttgcgg
    61561 gtaaaccgga caagattcct tgtactccat acggcatcat gaaaatgttt gaagcttatt
    61621 ctatcgatcc ggcgggtaaa cgagcagtag ttatcggacg aagcaatatc gtcggtaaac
    61681 cgatggctca gctactcttg atggcagatg caactgttac tgttgctcat tctaaaaccg
    61741 aaaatctggc tgaactggca caagaagcag atattttagt cgtagcaata ggacgtggac
    61801 attttgtcac aaaagaattc gtaaaaccag gtgctgtagt gatagatgta gggatgaatc
    61861 gtgatgctaa cggtaagctg atcggtgatg tgaaatttga tgaagtggag cctattgcag
    61921 gctggatcac tcctgtgcca aagggtgtag ggccaatgac tatcacgatg ctgatgtatc
    61981 aaaccgtaaa atctgccgaa gcttttggag caggtgaata gatggcacaa gaatatttaa
    62041 ctgtcactac attaacgaag tatttgaaaa gaaaatttga tgccgatcct tatttagaac
    62101 gcgtatattt aacaggcgaa atatcgaatt tccgtttacg tgccaatgcc catcaatatt
    62161 tcagcttaaa agatgaccat gcaaaaatat ctgctattat gttcaaagga gcattccaaa
    62221 aattacgatt ccagccaaaa gaaggaatga aagtccttgt cgttgggcgt atttcattat
    62281 atgaagcggg aggttcttat caaatctatg tagagcacat ggagcctgat ggaataggtg
    62341 ctttatatca agaattggaa gaacgaaaag aaaagctgat gaaggaaggc ctttttcaag
    62401 ggcctaaaaa aatgctgcca cgtttcccaa agcgaatcgc ggtccttacc agtccaagcg
    62461 gagcggttat ccgcgatatc atcacgacgg tgaaaagaag atatccaatt gtccaattgg
    62521 ttttgttccc aacagttgta caaggaaacc aagcagcgga caatgttgtc aggaatatcc
    62581 agagagtaga agagatgggc aatttcgata caatgatcat cggtcgtggg ggtggttcga
    62641 ttgaggattt atggccgttt aatgaagagc gggttgctcg ggcgatttat caagctaata
    62701 cgccggtgat ttcatctgta ggacatgaaa cagataccac gatcgctgat ttagtagcag
    62761 atgttcgggc tgccactcca acagcagctg cagaattagc tgttcctgtt ttgtcagaag
    62821 agttgatgcg cattcaagaa cggcaagcta gattgcagca ggcgtttgta cgccagatcc
    62881 agcgcaaaca agaacggttc gagcgtgctc gacgttccta tgtatttcgg cagcctgaaa
    62941 gattgtatga aggtcagacc atcaaattag accagttgaa acagcgatta tatcaagcta
    63001 cacagcaaat ttatcatgcg aaagaaaaac aaacgatcgc attaagccac caattggaac
    63061 aagtggctcc gctttatcga gttaaagcag ctcgccaaga aactgcttat ctagaaaaac
    63121 gcttagaaga gaaaatccaa caatatatga aacaacagca gcagcgtttt gctcaagctg
    63181 ttcaatctct tgatttgttg agtccattga aaatcatggg aagaggctac agctatacga
    63241 cgttaaatga tcaagtagta aaaagtgtca ccgagattca tcaaggggat ttgctgacga
    63301 tccattatca agatggaatc gttaaaggac aagtacaaga gatagacaaa ggagaataaa
    63361 aatatggaaa atccaacttt tgaagagtca ctgcaagaat tagaaaaaat tgtcatgcag
    63421 ttagaacaag gagacgttcc attagaatca gctctagatt cgtttaaacg aggaatggaa
    63481 ttaagcaaac attgccaaga tacattagct aaagcagaag agaccttgac gaaaatgatg
    63541 acagaatcaa atgaagaagt tgattttgac ggaaacgagg aaagctgaga tgagattaac
    63601 tgacttttca gctattcatc tacctgctgt agaaaaagaa atcttgtctt ttttagatga
    63661 gcatacaacg gatagaggac tctatgatgc gatgagttat tcagtgaaag caggcgggaa
    63721 aagaatacgt cccttattac ttttaacggc cgttgcttcc tttgatgagc ctattgatgt
    63781 tcccgtctat caagtagcag ctgctttaga aatggtgcat acgtattcct tgatccacga
    63841 tgatttgcct gcgatggaca atgatgatct tcgtagagga aaacctacca atcataaagt
    63901 attcggcgaa gcattagcta tattggccgg agatggtttg cttacaggag cattccagct
    63961 aatcagtatg gcacacttag gtaattctcc taagcttctg ctgcttcagc aattagctgt
    64021 ttgtgcggga tcacaaggta tggttgctgg gcaagctgca gatatcgagg gcgaatcgaa
    64081 aaagctttct ttagaagaat tagcatttat tcatgaaaga aagacagggc atttgattcg
    64141 ttatgccttg ctggctggtg gcatattagc gaagcaacct gaagaaatct tattgctact
    64201 tcaacgatta gccgaacatt taggtttagc ttttcagata agagatgatc tactcgatgt
    64261 gattggtaca acgaaaacat tagggaaaac agctggtaaa gatgaacgta tggaaaaaaa
    64321 tacttacccg cgtttattag gcctagaaaa gacacgtgag gcgttggaga ttgagctgtt
    64381 atccgccaac aagatcatag acaaattaga agaggacgtt ttttcttttg atggcgggct
    64441 gatgcgccag atgatcaagc aatttgatgt ggagaaatga gggaaagaat ggaaaaagaa
    64501 cgcgtagatg tattagcggt caaacaagga ttatttgaaa caagagaaca agccaaacgt
    64561 tcagtgatgg ctggtttgat ttacaacgaa aaaaacgagc ggtttgataa accgggagaa
    64621 aaaattccag tgagtagtga attaagagtc aaaggtaaaa aacttcctta tgtttctcga
    64681 gggggattaa aattagaaaa agcactcaaa gcatttgatc tatctgttga aggaaaaaca
    64741 ttgttagata ttggtgcatc cactggcgga tttactgatg cagctttgca aaatggcgca
    64801 aaaatgagtt atgctttaga cgttggctac aatcagctag cttggaaaat aagacaagat
    64861 cctagagtcg tcgtaatgga aagagtgaat tttcgttatg caaaacccga agactttacc
    64921 ttaggaattc cagaagttgc tgttatcgat gtttctttta tttcactaaa actgatgctg
    64981 cctccattac atgctatttt aaaagaagag ggagaagtga ttgctttaat caaaccccaa
    65041 ttcgaagcag gcagagaagc tgtagggaaa aacggaattg ttcgtgaccc tcagacccat
    65101 caaaaagttt tagaagacat tttgtcattt gcggcacaag gtggatatga tgtgctggaa
    65161 ttaagttatt ctccgatcac ggggggagaa ggaaatatcg agttcttggc tcacttgagg
    65221 aaagtacccg aaagcggaac aatcaattca gctattaata tggcagaagt ggtttcaaat
    65281 gcacacgaac agtttgacca taaataaatt gtaactgtga aaatcaagtg agaagtatgc
    65341 ggattcttct ttattatacc aaggaaataa gctatcattg attaagaaga atatgctaag
    65401 gagcgaagac gtaatgagaa aaaaagatcg gcatcgtttg attactcgat tgttgagtga
    65461 gcaagatatc cgaaagcagg aagaatttgt tgaaatcctg aaaagcaaag gaatatctgt
    65521 tactcaagct acgatttcca gagatataaa ggaactgaaa ctgatcaagg taccggcttc
    65581 agatggcggc taccgttata gcataccagc tgaaacaaac gaagatgtaa atactaaatt
    65641 ggaaaaacta ttaaaagacg ctttcgtggc agttgaccaa atggaaaaat ttgttatttt
    65701 gaaaacattg ccgggaaatg cttcggcagc agcaaatttg attgataaac gttacaagaa
    65761 agaattattt tcgattatca atgatgacga taatgttttg atgattacac gaactgaaga
    65821 ccaggcagtt ttattgaaga aagacttcct gcattacctt taagttgagg agcgaatgtg
    65881 atgctacaag aaatcagtat tactaacttt gcgattattc cggaattgcg gctttctttt
    65941 cacgaaggaa tgaccgcgct gaccggagag actggtgcgg gtaaatccat tattatcgat
    66001 gctttaggtc tgttagcagg aggaagaggt tcaagcgatt atatacgtca aggagcagaa
    66061 aaatgtgtgc ttgaagggtt gtttgaacta ccaaaacaag aaggattctc tgaactgatg
    66121 gttgaattag ggatcgaaac agatgaggac aatctgattg ttcgacggga tatgtcctta
    66181 actggaaaaa atgtttgtcg agtgaatggt catattatca cattagctaa tctgagaaga
    66241 atcggcagtt atttagtaga tatccaagga caaaacgaac atcaggaact gctccagcct
    66301 gaatcacatc ttgctttatt ggatcgtttt ggagatgtag ctttccaaca gaagaaaaag
    66361 agttatcagc aagaatatat ctcttatcgt gagctggaaa aaagggtacg aaaagtgcag
    66421 caaaatgaaa aatcctatgt tcagagaata gatatgctcc atttccagca agaagaaata
    66481 gctgctgctg atttgcaagt cggagaagaa gagaaattaa aagaagaaag agaaaaactt
    66541 agcaattatc aaaaaattgc tgatggatta gctgcaggtt acggagcact gacgaataat
    66601 gaacaaaaca gtgtagatgg tgttggcttg gccgtttcag aactgcaagg gatcgcccat
    66661 ctagatgtgg agtatgaagc aatttatgaa aatatccaaa gtgcttacta tttgctgcaa
    66721 gatgcaatcg gcgatatgag tcgtcagatt gatttattgg agttagatga gagtcgttta
    66781 gaagaagtta cgcaacgttt agaattaatc cgacagttga aacgtaaata cggcgaatcc
    66841 attgagtcaa tattggctta ctatgacgaa atcaccgaag agctagcttc ttctgatttt
    66901 tcagaaggtc aattggacaa aatgaaagag gaactagagc aaaaagagct tctacttcag
    66961 caacaagccg cagatcttca tgaagcgcgt aaaaaaatcg caaaagaatt ggaaaaatcc
    67021 attctgcatg aattgaaaag tctgtatatg gaaaacactg agtttgaggt acgtttctta
    67081 aaagaagaaa atagacaatt gaatcatgat ggatttgacc aaatcgaatt ttacattacg
    67141 accaatccag gagagccact gaagcctttg gtgaaagtcg cttctggtgg cgaactgtct
    67201 cggatgttgc ttgctttgaa aactattttt tcttctgagc aaggtgtgac aagtattatt
    67261 tttgatgaag tggatacagg tgtgagcgga agagttgcac aggccatcgc agataaaatt
    67321 tctcaaatat cgaaatactc tcaagttttg tgcatcactc atctgcctca agttgctgca
    67381 gtcgccgact atcaatatta tattgtgaaa gcagtgattg atggacgtac gcaaacttct
    67441 gtttcagagt tgaaaacaaa agaaagagaa gaagaaattg cacgtatgct ggctggaagt
    67501 gagattacaa aactcacact cgaacatgca aaagaactat tgaaactcgc aaaaaggacc
    67561 tgagccacct cgcagtggtt caggtccttt tcagcagctg atagctatat tttattttta
    67621 catgacactc attacagcag tcaatacaac agatccaatc gttaaaatca gcattgtcca
    67681 aatgacaaat tttgttactt tagcaaaagt acttgtttgt tttttttcac gcattgtttt
    67741 caacctcgtc attaattagt tgattcaagt gtacaatctt ttttccattt ccacaagagg
    67801 gcttttttat aaaaacttcc ctttttttaa ataataggta gttacgatac aaataaataa
    67861 tgttaataga actagccata taaaggcatc atccctttca gcaataggta acttaatgtt
    67921 cattccatag atactgccga cgattgtcgg tatagttaaa ataatcgtta aagaagttaa
    67981 aattttcata atattattca gattattcga tacgattgct gaaaaagtct gattcatttg
    68041 ttctactagt tttaattgaa tgcgagaagt agtcattgcc tgctctgttt ccaccaatac
    68101 atcacgtaaa tgatttttca aagcatgatt ttcttggaaa agtttatttt gcgctagttc
    68161 tttcaatgca tctaaattag cagaagtcgc ttctctgaaa taaacgaggc ttttttggat
    68221 atccatgatt tgataaagtt gtttgttttc agtggatact tgaagtttgg cttccaaagt
    68281 atcgcattga gattgaagcg tagctaaata actgttgtaa gagagtgtga cctgccaaat
    68341 gaattgaaag aagatattga gattaggcga aatatcactt tttggaaagg tctggctaaa
    68401 gactttcttt aaaaacagtg gttcatgatt cgtaattgtg ataagcttct ttttgggcgt
    68461 caaaataata gagagcgggt atgtatccac ttgaaaataa ccgctaggac ttgttttagc
    68521 aaaaggatat tgtaacaata ataacgctgg ctttgtcaac tttgattgat tgaattcttc
    68581 tgtacgggaa ttttctgcat cgtccaatat ggaagtgatg taatctgacg gaatttcaaa
    68641 ttgatgtgtc agctgatgaa tctcttcttg attaggacgt tctacacaat accagatcgt
    68701 ttggtcagtt tcctttccgg tattacgcag tttctcatta tctaatgtaa aatatttgat
    68761 catgattttc ctcctgttgc ctgtttcttt tattataacg gatagggaat tggaatacat
    68821 ggaatgtgtt tgtaagatat agaatataac tgcataaatt taaataaaat gaaacagttg
    68881 tgtaaagtat atacagtata cttgaagcaa cgataaaaaa ggagtttacg ttcatgggca
    68941 cattcacacc acttatcttg ttttggtata tttacccggt aatcgttttg tttggttgcc
    69001 aatttcttgt aaaattattt tcgttaaatc gtcgttttaa aataaaagcc cctgatttgg
    69061 cgattccttt tttgtggggc ggtatgcatg cattgtctag aaatacaggg acaatttcta
    69121 tcttaccgtt tttctttatt tctgttttgc taatgggaat ttgtattgct gttttccaag
    69181 catattatta tgaagaaatt atttatccgc gttatttcaa gatgacttgg cgtttggttt
    69241 ttctgttgac aatcgtcttg tacgctgtct tgatcattgt gaacatactt tcttacctat
    69301 gaaaaaaata gaaaagctgt aaaaagtctt ttaaaaggct ttttgcagct tgatttttaa
    69361 aaaaatgttt atgaataaaa aaagttgttc ccggatttcc cagaaaaggt ggtagaaagt
    69421 gggggattgt ggtagactaa ttttagttag tggaggaatg gggggctttc aatgttgatg
    69481 ggtgaattcc aacataatat tgatgcaaaa ggacgtttaa tcgtgccctc aaaacttcgt
    69541 gaagaacttg gcgaaaagtt tgttctgaca cgcggattgg atggctgtct tttcggctat
    69601 ccgatgagcg aatgggaaaa tctggaagct aaactgaacg aaatgccgtt ggctaaaaaa
    69661 gacgcacgta cttttgtgcg attcttctat tcggctgcca ctgaatgcga acttgataaa
    69721 caaggacgaa tcaatatacc cagcacgtta agaaattatg cagctttgac aaaaggttgc
    69781 gtagtcatcg gtgtatctaa ccgaattgaa atttgggatg agacacgatg gcaagatttt
    69841 tctgcagcag cagaagagaa ctttgacgaa attgctgaaa gtatgattga tttcggacta
    69901 taagaatagg aagaggctga ctaatgactg aaacatttca acactataca gtcatgttga
    69961 aagaaacggt agatggattg aacatcaaac cggatggcgt ttatgtagac tgtacactcg
    70021 gcggtgccgg acatagtgaa tatttattaa gccaactaag ttctaaaggg catctgtatg
    70081 cgtttgacca agatcaaaaa gcaatcgatc acgccaagct gcgtttagct tcttatgtcg
    70141 aacaaggtat ggtcacattt atcaaagcga acttcagaga attagaagaa cgcttgagtg
    70201 agcatgtaga taaggtagac ggcattttgt atgatctagg ggtttcctct cctcagctag
    70261 atgaagcaga acgtggattt agttatcatc aagatgcacc gttggatatg cgcatggatc
    70321 agtcagctcc tttatccgct tatcacgtaa ttaatgaata ttcctaccat gaattagtaa
    70381 agattttctt ccgctatggc gaggagaaat tttccaaaca aattgctcgt gaaatcgaac
    70441 gagtacgaaa aacaaaacca atccaaacaa ctggtgagct tgtcgaaatc attaaacaag
    70501 tcatcccagc tcccgcaaga agaaaaggcg ggcatcctgc aaaaagaatt tttcaagctg
    70561 tgcggattgc agtaaatgac gaactcggtg ccgaagaggc ctctttagaa caggcaatcc
    70621 ggttattgaa agtcaatgga agaatcagcg taatcacatt ccattcattg gaagacagaa
    70681 ttgtcaaaag tatgtttaaa gaatatagta cggtacaaga tctaccaccg ggacttcctg
    70741 tcatacctga cgaattccaa ccagaactga aagtcattac tcgtaaacca atccttcctt
    70801 cagaggaaga gctaacagaa aacaatcgat cacgcagtgc aaagttacgt attgcagaaa
    70861 aaataagaat gaaagaggag tagaatgatg gcagaattaa agaaaatgca agattacgaa
    70921 tatgatttac ctgaattggt ggatcagccg gaacaacaaa tgcctgattc ttcttcgaat
    70981 caagaaatca ttcttccaca atcaccggca aaacgattaa agcagatttc tgctttggaa
    71041 aaaattgctg tggcaagcgt ggcgattgct attgttgctt tatgtgtttt aacagttatg
    71101 ctgcgcacaa atatcagcgg agctgaaaaa gaaatcacgt caatacaaac agaaatcaat
    71161 gataaaaagc aagaaaaaac gagtttagaa caagaaaaaa acgaactttc tcgtactgaa
    71221 cggattaaac aaatcgcaga agaaaaagga ttgtctatta atgacgacaa tttaaggaaa
    71281 gtgaagtaaa tgagtcttaa aaataaaatt cggcggttta tggaaaagaa aaatctaaat
    71341 ccgatgaaca atcgcaaaaa agtaggtatc attttatttg ctacgagtat tggattgttc
    71401 tttttatttg ccgtccgttt ctcgtatatc gttattggag gtcatgttgc tggtacctca
    71461 ttagctgaaa aaacaaaaca gttgtaccaa ggaagtgaag tagtaaaagc aaaaagagga
    71521 acgatttatg atcgtaacgg tgtggcgctc gcagaagatg cctcctctta ttcaattaaa
    71581 gcgatactct caaaaacata tacctctggg gataaaaaac tgtatgttga agaaaagaat
    71641 tttgataaaa tagcagaaat cctgcataag aatctttcta tagacaaaaa agatgcactg
    71701 aatattttag aggacggcgc aaaaaaagag ctgtatcaag ttgaatttgg tagctatggg
    71761 aaaaatatct cccaagaaac caagcaaaac atcgaagcgg acatgaaaaa agaaggtgtc
    71821 gctggtcttt attttgtcga ccatcaggct cggatgtatc caaatggcgt cttttcttct
    71881 cattttattg gatacgcagt tccagacaaa gatgagaatg gcttagttgg taaactaggt
    71941 ttggagtctg cttacaatga tatcttgagc ggtaaagatg gtaagattat ttatcaaaaa
    72001 gataattttc aaaatcctct gccaggtaca gtggcagaag aagaaaaagc tgtcgacgga
    72061 caagatatct acacgacttt agacagcaga ctacaaagtt atttagaaac actgatggat
    72121 caagtaaatg aagaatatca gccagaagaa ttgacagcag tcctgatgaa agcaaaaacc
    72181 ggagaaatct tggcaatggg acaacgacct actttcaatc ctgaaacaat ggaaggattg
    72241 actggaaaag atgctatctg gcgtaatttc ttggtacaag atagttatga accaggatca
    72301 accatgaaag ttttcactac tgcagctgct atcgaagaag gagaattcaa cgagaatgaa
    72361 accttccaat ctggaaaaat ccaagtagaa gatgctacga tcaatgacca tgactttggt
    72421 gaaaaaggtg tgctgaccat gcgtcaagct ctttcatggt caagtaacgt cgggatggtc
    72481 atactggaac agcgattagg cggacgatgg tataactatt tacaaaaact gggatttgga
    72541 caaagtactc attctggatt agatgatgaa gtaaacggtg ctttacctac attaaatatt
    72601 gtcgaccgag caatgagtgc ttatggacaa gcggtaggtg tgacgaattt ccaaatgatg
    72661 aaagctttca cttctattgc gaataacggt acaatgattc aacctcgata tatcagcaaa
    72721 gtggtggatc cccaaactgg agaagagcga acaactcaaa cagaagttct agggcaacct
    72781 ttttcaaaag aaacaacaga aaaagtccgt gaatatatga gagatgttgt cgaaagcgaa
    72841 aactacggta gtgcttatgg tgtatatagt gtacctggat acaatgtatc ggcaaaaaac
    72901 ggaacagccc aaatcgcttc tgatacaggg ggatatcaaa caggagatac agcctacctg
    72961 tattcgattg tggagatggt tccttctgaa gatccagact atgtcttgta tctaacaatg
    73021 aaacacccta agacatatga tcgaatggca ttggctaaaa tcgcaaatcc attgatgaaa
    73081 cgggcaatgg attttaaaga aactgaagaa gattcagata cagaaacaaa aacagaaaaa
    73141 gtatccgtag ccgattatcg taatcttgaa gcggacgttg cagcagctga tgctcaaaaa
    73201 agtggactgc aacctgtagt gatcggtaat ggtaaaaaag tacaaaaaca atctactgcc
    73261 aacggtgatc agttgatctc tggagaaaaa ctgatcctat atactggtgg tgataagctg
    73321 atgcctgacg taacaggatg gtcaaaagca gatatcatga agctggggaa aatcctcggt
    73381 attgaagtta gttttgatgg agatggttat tgtgtcaaac aagaacttgc tccatatgaa
    73441 aaaatcactg aagacaaact tagttttact ttagaagaat aagaagagaa ataggagaga
    73501 aaagagcatg gaatggacac aagcattgat tcccattgtc agcagttgtg caatgacaat
    73561 tgccgcaatg ccgctattta ttggttattt tcaaatgaaa aagcaaggtc aggcgattcg
    73621 tgaggaggga ccaaagtggc acaatagcaa agccggaact cctacaatgg gcggcttagt
    73681 gtttctgatc ggctctattt tgacaggaat ttgggttgga gcttggcaaa aacagctaac
    73741 tcctactctt ttcatcttgc tttttgtatt agcgttatac ggagtaatcg gctttcttga
    73801 tgattttata aaaatattca agaaaagaaa catgggactg aactcgaagc agaaattgat
    73861 tggacagatt ctagggggaa tcatctttta tcttgtttac cgttctgaag gttatccagg
    73921 tgtattgaat ttcttcggac tagatctacc tttaggaatc atttatggta ttttcgctct
    73981 cttctggctt gttggatttt caaatgcagt aaatctgaca gatggcatcg atggattagt
    74041 tgctggctta ggaagtatct cttttgcagc ttacggtcta attgcttggc atcagcatca
    74101 gtatgatgta ttaatcattt gcttaagtgt tttaggaggt ttactgggtt tcttcgtcta
    74161 caacagaaaa cctgccaaga tttttatggg agatgtcggt tcattagcgt tgggcggact
    74221 tttagcggca atttctataa tgctaaacca agaatggaca ttgcttttga ttggtttgat
    74281 ctatgttatg gaaacagcaa gtgtgatgct tcaagttact tcctttaagc ttacaggaaa
    74341 acgaattttt aaaatgtctc caattcatca tcactttgaa atgtgcggat ggtcggagtg
    74401 gaagattgat acagtttttt ggttgatcag cattgtgact tcgttgatca cattatggat
    74461 cgtttggtag gagatgacgg gaaatgaaaa aaatatcaac atatgaaaat aagaaagtgc
    74521 ttgtcttagg actcgcaaaa agcggagtaa gtgcggccaa attattacat gaattgggcg
    74581 cacttgtcac cgttaatgat ggaaaacctt ttgacgaaaa tccggaagca caagagctgc
    74641 tttctttagg gataaaagta ataacaggta gtcatccaat cgaattgttg gatgaagaat
    74701 tttcattgat ggtcaaaaat ccagggattc cctacagtca tcctttagta gcaaaagccc
    74761 aagaaatggg tattccggta attacggaag ttgagctagc ttacgaagta gcggaatgcc
    74821 caattattgg gattacagga acgaacggaa aaacaacgac aacaacgatg acaggactac
    74881 tactgaatgc aggagcagat caaggaattg cacgtttagc gggtaatatt gggtatccag
    74941 caagtggtgt agcgcaagaa gcaaaatcag aagataaaat tgtgatggaa ttgtctagct
    75001 tccaactcat gggaatcaca gatttccgcc ctcatatagc ggtgattacc aatatctatg
    75061 aagcacatat tgattatcat ggtacaagaa aagaatatgt caaagctaaa tggaaccttc
    75121 aaaaaaacat gacagaaaaa gattatctga tccttaactg gaatcagtca gaactacaag
    75181 aattagcgca aagaacaaaa gcacgcgtac tgcctttctc gacaaaagaa gtgttggaag
    75241 acggtgtgta tgctgacgat tactcaatat actataaaaa agaaaaaata atggaaatat
    75301 ctgaacttgg cgtaccaggt aagcataatg tagaaaatgc actagctgct atttctgtag
    75361 caaaattgta tggtatatca aatgaagcta tacgagaaac gttgcatttt ttccatggcg
    75421 taccacatcg tacgcaatat gtaggagaaa tccaaggcag aaaattttat aatgattcaa
    75481 aagcaaccaa tattttagcg actaaaatgg ctctaagtgg atttgaaacg tctaaggtcg
    75541 tgttattggc tggcggatta gacagaggaa atacatttga tgaattaatt ccttcattaa
    75601 aaggaatcaa agcaatggtc gtatttggcc aaacaaaaga aaagttgatg gatgctggaa
    75661 aaaaagcagg catcgagaca atagtaacag ccgattctgt agaacaagca gttcctttag
    75721 cattagaaaa cagtacagat ggtgatgtag ttttattatc accagcgaat gccagctggg
    75781 atcaataccc aaattttgaa actagaggaa acagattcat ggaagctgtg aaccgattaa
    75841 aatagaaaaa gtagaagcga gcgataaaaa atgaaaattt tggttacagg cggaggaaca
    75901 gggggacata tttatccagc tcttgccttt gtcaattatg taaagactca agaaccgaat
    75961 gctgaattca tgtatgtggg agcaaaacga ggtctagaaa acaaaatcgt ccctgataca
    76021 ggaatgcctt tccatacctt ggagattcaa ggatttaaac gaaaaatttc catgcataac
    76081 atcaagacgg tccgattgtt tcttaagagt atccgtcaag caaaaaaaat cctcagtgat
    76141 tttcaaccag atatcgttat cggtactgga ggatatgttt ccggagcggt agtgtacgct
    76201 gcagccaaaa tgggtattcc aacagttatc catgaacaaa acagtgtacc aggtgttacc
    76261 aataaatttt taagccgata tgtggatcgg atcgctcttt catttaaaga tgcggcttcc
    76321 ttttttcctg aaaacaaagc agttcttgta ggtaatcctc gagcacaaga agtagcagat
    76381 actaaaaaat cagatatttt gaaaacattt gatttggatc cagaaaagaa aacagtcttg
    76441 atttttggcg gtagtcaagg agcacttaaa atcaatcaag cagtatcagt ttttttaagt
    76501 gaatatattc cttcttctta ccaagttttg tatgcttcag gagatagata ttatgaagag
    76561 atcctaaata aaataaatcc tgaaatacaa aatattagtg tccagcctta tatcaaaaat
    76621 atggcagagg tcatggcttc cagtgatctg ctggtggggc gtgctggtgc aacttcgatc
    76681 gcagaattca cagctctggg gttaccggca gtgttggttc ccagcccata tgttacgaat
    76741 gatcatcaga ctaaaaatgc aatgagtctt gttcacgcag gagcagcaaa aatgattgca
    76801 gataatgaac taacaggtga atcactaagt cagacagtca acgaaatcat gggtgatgaa
    76861 gaattgcaaa agcaaatgtg ccgagcttct aaagaacaag gaatccctga tgcttcaaaa
    76921 cgtctttatg acttagtaaa acaaataatc aaaaaataaa ggcggtggtc ttagtgagta
    76981 agaaaaaaga accaaaaaaa ttgtctcatc cgtcttctga tgaaaacaat ttgactcctt
    77041 ggcaaaaggc taaccgaaaa tatctggcag aacaagagat gaaaaaccaa gagccaaaaa
    77101 aagaagacat aacattagat acaccccttt cagattctga agaagaaaaa acggttcaag
    77161 aggaacagca agaagaaagc cagtccgaga aaaaaatcga attttttgaa gagatcaagc
    77221 aaaagaaaaa cggtggacca tataacggtt cttttttgaa ccggctgccc aatttaaaaa
    77281 gtcaacggca caaagtacta tttcgtcggt tagctttgat catcactgta ttgggtatac
    77341 cgctgatttt tttgatctat tatgtttctc cttatagcaa attacaagca gttgcagtat
    77401 caggcaataa gacagtcaac agccaagaaa tcatctctga tacaaaattg tcattaggag
    77461 aaaatgtttg gggacagtat tttcatcgat ctacttatat cgatcgcctt aaaaaagcac
    77521 agccaagaat cgaaacagca aatatacatt tcaaagggat gaatgaattc gatttggatg
    77581 taacagaata taaagagatt gctttgatcg caaaaaacaa tcaatactac ccagtgattg
    77641 aaaatggaac agtattagat gaaaaagtag caaatcctac aaaaaatctc cctatcttgg
    77701 aagagttcaa agactctgca aaaatcaaag aattaacaaa gcaatacaat cagttatcat
    77761 cagaattaca aaaagcaatt tctgaaatca agtacacgcc aagagcaagc aataaaaatc
    77821 tgatccaatt gaatatgaat gacggcaatc aagttattgt aaatatcaat aatcttgcga
    77881 accaaatgaa atattattcg caagtagcca aagatatgga tgaaaaaggt gtgatcgaca
    77941 tggaagttgg tatcttctcg catccttacg gtagcactca agaaacggaa gaatcgggcc
    78001 aatcggaaga aacaactgaa agtgaagaaa atgcggatca aacaaacgaa tcaagtgaag
    78061 aggcagaaac gcaaaaagca caaacacctg gatcgtctag cacaaacgga tcatctgatt
    78121 caaatgattc aagtgaaaca agtcaaatag aagataatcc aagtacaact ccgtcaagtt
    78181 taaatgaaaa ttgataaaac aactttaaaa agtaagaatg tactttctaa aaaccagtaa
    78241 tttatggtag aattggaagc agtgaatata tctattaata agtatatttt aaaacggacg
    78301 ctagttgttc gcttgtatat ggaatagtaa gtaggaggga atcccattca tggcaaaaac
    78361 aggaatgtat gtaggccttg atattggaac gacatctgtt aaagttgtcg tggctgaata
    78421 tatcgacagc caaatgaata ttattggagt aggaaacgca aaatcagaag ggattaaccg
    78481 aggcattatc gttgatatcg ataaaacggt ccaagcgata caacgagcag tacgacaagc
    78541 agaagaaaaa gcaggtatcc agatcaaagg cgtaagtgtc ggtttgccag caaacatgct
    78601 tgaagtagaa aattgccagg gaatgattgc agttaacgga gattcaaagg aaatcaccga
    78661 tgaagatgtc agaaatgtgg cttcagctgc ccttgttcgt tcaatacctc ctgaacgtca
    78721 aatcgtgtca atattgccac aagattttac tgtcgatggg ttcgagggaa tcaaagatcc
    78781 tcgtggaatg atcggcgtaa gattagaaat gtacggtctg ttgtttacag gacctaaaac
    78841 gatcatccat aacatacgta aatgcgtgga aaatgccgga ttaatcgtca acgaaatggt
    78901 gattactcct ttagcgctga ctgaatcaat tctttctgac ggagaaaaag attttggtac
    78961 gatcgtcatt gatatgggtg gcggccaaac aacaacggct gttatgcatg ataagcaatt
    79021 gaagtttacc aatcttgacc aagaaggtgg cgaatttgtc actaaagata tctctattgt
    79081 attaaatacg tcatttaaca atgcagaagc gttgaaaata aactacgggg atgcataccc
    79141 agaacggaca tcaccagatg aagaatttcc agtagatgtg attggtcagt cagaaccagt
    79201 gaaagtcgat gaacgctact tgtcagaaat tatttcagct cgaatggaac agattttcaa
    79261 caaagcaaaa gatgcattgg accaaatcga agcattagag ttgccaggtg gcgtcgtatt
    79321 gacaggcgga gctgctagtc ttcctggtgt tgtggacttg gcacaagaaa tcttcggcgt
    79381 aaatgtaaaa ttgtatgttc caaatcacat ggggctgaga aacccggtat ttacaaatgt
    79441 aataagtatc gtggattatt cagcaaactt gagtgaagtg tatcaattgg caaaaattgc
    79501 tgttacgggt gaaacctcag cagctcgtca aatggtagtt gaacaagaaa caacaaacac
    79561 atatgagagc tatgaagcac ctgaagaaac aatctacgat gagccggaac caaaagagtc
    79621 cggagaaaat gtaaaaaata aaatcaaggg tttctttaca aatatttttg attaattcat
    79681 cctgggagga ataagatatg gaattctcaa ttgataataa tatcaatgac ggcgcagtaa
    79741 tcaaagtcat cggagtcggt ggtggcggtg gaaatgctgt aaaccgaatg attgaagaaa
    79801 acgttaaagg tgttgaattc attacagcca acacagacgt gcaagcatta aaaaattcaa
    79861 aagctgaaac agttatccaa ttaggcccca aatatactcg tggtttaggt gctggttctc
    79921 agccagaagt tggacaaaaa gcggcagaag aaagtgaaca atccttacgc gaagctttag
    79981 acggtgcaga tatgattttc atcactgcag gaatgggtgg aggaaccggt acaggtgctg
    80041 caccaatcgt tgcaggtatc gcgaaagaat tgggcgcatt gactgttggt gtcgtgacac
    80101 gtccattcac ttttgaagga ccgaaacgtg gccgttttgc tgcagaaggt atcgctcgtt
    80161 tgaaagaaaa tgtggataca ttattaatta tctcaaataa ccgtttatta gaagttgtag
    80221 ataaaaagac accgatgctt gaagctttcc gtgaagcaga taatgtttta cgacaaggtg
    80281 tacaaggtat ctctgatttg atcacagcgc caggttacgt taaccttgac tttgctgacg
    80341 tgaaaacagt gatggaaaac caaggtacag cattgatggg gatcggtgtg gctagtggag
    80401 aagatcgcgt agtagaagca acgaaaaaag caatctcctc tccattgctt gaaacatcta
    80461 ttgatggagc tgaacaagta cttcttaaca tcacaggcgg tttggatatg acattgttcg
    80521 aagcacaaga tgcttcagat atcgtagcaa atgcagcaac tggcgatgta aatatcattc
    80581 taggtacatc tatcaatgaa gaaatgggcg atgaaatccg tgtgacggtc atcgcaacag
    80641 gtatcgatga atcaaaaaag gaacgcaagt ctagccgacc agctagacaa gcacaaatgc
    80701 aatcacctgc tcaaaagact gtattagata tggatcaagc taaaccaact tcttcagaag
    80761 aagaaaacag ttttggcgat tgggatatca gacgtgaaca aaatgttcgt cctagagttg
    80821 atgattccaa ttttgacaat atcgaaaaaa aagaatttga tacattcaac cgcgaagaaa
    80881 caaaatctca tggcgatgat gaattaaata cgccaccgtt tttccgtcgt aaaaaataaa
    80941 ggagaggaag agcacatgat tgctgacaac ttgcagaaaa tcaagcaaga aattcaggat
    81001 tcgtgtgctc ttgttcatcg tgatccgaaa gaagttacat tagtagctgt aacaaaatct
    81061 gttgaatccg ctcaagctga agaattggtt agagctggcg ttatgaattg tgcagaaaac
    81121 cgagtagata aattattaga aaagaaacaa gatttatttg attttcctga aataaagtgg
    81181 catttaattg gtaatttaca gcgcagaaag gtaaaattga ttgtaaatga aattgattac
    81241 tttcatgcgt tggacagttt aagattagca gaagaaatcc agaagcggtt agagaaagaa
    81301 ttatcctgtt ttattgaagt aaatgtttct ggtgaggaat caaaacatgg aatttcacca
    81361 gatgagcttt taccgtttgt ggaatcttta gctgcctgca caaaaatcaa ggttgttggg
    81421 ttgatgacta tggcgcctaa agaagcttct gatgagcaaa tccataaagt gtttggtacg
    81481 ctgaaagaat tgcgtgatct tgttcaagca aaaggttatg cccatgcacc atgtagtgaa
    81541 ttaagtatgg ggatgagtca agactttcaa attgcgattg aagaaggtgc aacatttatc
    81601 cgtgtaggaa cagcgctatt caaagacgaa taaaggagga acaaaaatgc cgcttttaaa
    81661 cagaggagca attgctaatt ttttcggatt agctgatgag gaagagtacg aataccaaga
    81721 ttatcctgaa acaacaaaaa aggctgtaca gccaactaaa aaaaatacaa cacagaatca
    81781 atcaactcat tctgtatctg ctgcgaatac tcatgaaaaa caaaaaacgg cgacttcacg
    81841 tcctgtaacg catgaaagta ttcagccggc tgaaaagaaa aaagtaattg aactacatca
    81901 atcacctaca aaaactgtag cgaaagaaag aacagcttca agaacctcta cacaacctaa
    81961 agcaaatctt gctggaaaaa tcactgtaat ggaaccaaga gcttattctg aagctatggc
    82021 gattgctaaa cgaatcattg ctggagaagc aaccttagtg aattttcatt tagtagaaga
    82081 aaaacaagcg agaagaatcg ttgattttct aacaggaaca gtttatgcac tagatggaga
    82141 tatcaaacgt gttggagatg agatttttct ttgtacaccg actgggatgg agattgacag
    82201 ttcaaccgct caatcttttg cagatgcgaa tttgtttgat ctgtaagagg aggaactacg
    82261 atttatacta ttgcactatt acttcattta cttcaacggg cagcctattt ctatagcatt
    82321 atcttgatgg tatatgcatt gctctcttgg tttccaggcg ggtatcaatc agggctagga
    82381 cgattattag ctaagatctg cgaaccgtat atcagtttgt tcgatcgtct gcctttgagg
    82441 attggaccct tggatttcac aattttaggg gcaatcctga tcttgaactt agccgtacaa
    82501 ggtttagcac agatcgttat atggataact gtccatttag tttattgatt gaagcaacaa
    82561 aatctttatt ctcagaatag ataggagggg aaaattttgg atgcaaatgt ataccagcac
    82621 tttcgggcag atgaacgtac atttatcgat tcggtaggcg attggattga acaggttcaa
    82681 ggacaatatg caccctatct gaccgaattt cttgacccaa gacaatccta tattttagaa
    82741 acactgattc gacaaagcag tgatttgcgt ttcatgtttt atggcggata tgaacaggca
    82801 gaaagaaaaa gatgtttgat tttccccgat tattacgaac ctgtagagga agattttgaa
    82861 acagcattgt atgaaatcca ttatcctagc aaatttgcca gcctaagtca tgggaaaatc
    82921 cttggaactt tagtgggaac ggggatcaaa cgctcttact ttggcgatat catctcggac
    82981 ggtgatcgct ggcaagtatt tattgcaaat gaggtggatc attttgttgc tgcacaagta
    83041 acaaagattg gaaaggttgc tgtacgcttg gaaccaatca aatacacaga aatgattatg
    83101 ccgaaagata gctggacaca agaaagagga acagttagtt ccatgcgact agacagtgtg
    83161 atctctgaga tttataatat ttccagacag cggtccaagc agctgatcga agctggtaaa
    83221 gtaaaagtga actggacaga aaatacaaga cctgattttg tacttgaact attagatatt
    83281 atttcagtca gaggttatgg cagagtccag atccaagaac ttgaggggaa gacgaaaaaa
    83341 gaaaagtaca gggtactgtt cggtgtatta cgtaaataaa ataaaaaggt ggtttactca
    83401 tggctttaac tccattagat atccaaaata agacattccc gacaaaaatg cggggatata
    83461 atcaagacga agtagatgat ttcttagatt tagttgttcg tgactatgag gaattgactc
    83521 aaagaaatcg agaattagaa aaagcagtca aacattctga agaaaaactt gagtacttca
    83581 atgaattaaa agatgctttg aaccaatcaa tcatcgtagc tcaagatacg gctgataaag
    83641 taaaaacaag tgcaagcaag gaatccgaag tgatcgttac ttctgcacaa aacaaagcag
    83701 atgagttagt tgcgaacgct gaaaaacgtg cacatcaatt aacaactgat gcagaagaaa
    83761 aagcaagaaa aatcttgaca gatgcaacag aaaaagcaag acagcttgcg acagaaactg
    83821 aagatcttaa gaaaaaaaca cgagtattcc atcaacgtat cagcttgatg cttgagtctc
    83881 aattagaaca agtgaaaagt ccggaatggg atgaaatttt acaaccattc tccagctacg
    83941 tgactgacag ccatgaagtg atcaaagaag ttttagcaaa acagcttgac aatgaaaatg
    84001 atactgatgt aaactcagac acaggagtca tagaagcacc tatgacggca tttgatacac
    84061 aggctattga tatctcgatc gtgcctggtg aaaatgaaaa cgagtagaac agaaaagcac
    84121 tttgttattc ttaaagcgag ccgatgagag tggaagttcg gtaagacact gcatggtgaa
    84181 agatcctcta cttgagacct gcttttccga aattagagta agaagcagcg gctgacctcg
    84241 ttattggtca cagagagttt gcactagata gatgcaacta aatttgggtg gtaccacggc
    84301 atgttcgtcc cttgcggctg aacatgcttt tttgtgtccg gaaaacaaat aaaggagtgt
    84361 atggttgaat gaaaatgaaa gaaaccttgc aattaggaaa aacaaaattc cctatgcgtg
    84421 gtaatttgcc aaaccgagaa ggcgagtggc aaaaagaatg ggaagaaaat cgaatctacc
    84481 aaaaaagaca agagttgaac gaaggaaaac ctagctttgt tcttcatgat ggacctccat
    84541 atgccaatgg taatatccat ttaggacatt cattgaataa aatcagtaaa gacattatca
    84601 ttcgttcaaa atcaatgtct ggctatcgtg caccatatgt tccaggatgg gatacacatg
    84661 gattgccgat tgaacaagta ttggctaata aaggcgtgaa acgtaaagaa atgtccatgg
    84721 cagaatactt ggaaaaatgt cgtgaatatg cactttccca agtagataag caacgagcag
    84781 atttcaaacg cctaggagtt gcaggagatt gggaacatcc ttatgttaca ttagatcctt
    84841 cttatgaagc tgcacaagta cgcgtatttg gtaaaatggc tgaaaaaggc tatatttaca
    84901 aaggattgaa accaatttac tggtctccgt caagtgagtc ttctttagcg gaagctgaaa
    84961 ttgaatataa agatgtaaaa tctccatcta tttttgtagc tttcaatgtc aaagacggca
    85021 aagatattct tgatgaagat acagcttttg tcatttggac gacaacacca tggacattgc
    85081 cagcaaacca aggtattgct gtaaatccaa aatttacgta tgtattggtg gaagccgatg
    85141 gccgtaagtt tgtcgtagcg aaagatttgc tagaaacagt ccaatcagaa atcggttggg
    85201 aaaatgtaac agttctaaaa gaatttgctg gacaagaaat ggaatatatg actgcaagtc
    85261 atccattcta tgaacgtgaa tcacttgtga ttttaggcga tcatgttact ttagacgctg
    85321 gtactggatt agtccatact gctccaggac atggggaaga tgactatatc gctggtagca
    85381 aatataaact atctgtagtg tcacctgtag actcaaaagg attgtttaca gaagaagcac
    85441 ctggatttga aggtatcttt tatgacaaag caaatcctat gatcactgat ctattaaaag
    85501 aaaaaggagc tttgctgaag ctagatttct ttactcatag ttatcctcac gattggcgga
    85561 cgaaaaagcc tgtgatctat cgggcaactc cacaatggtt tgcatctatc gataaattcc
    85621 gtcagaatat tttggatgaa gtagaaaaag tagagtgggt tatcccatgg ggtaaaacac
    85681 gtttgtataa tatgatccgt gaccgcggcg attgggtaat ttcccgtcag cgtgcatggg
    85741 gagtaccact tccgattttc tatgctgaaa atggagaagc aatcatcaca ccagaaacaa
    85801 tcgaacatgt ggcaaaatta tttgctgaac atggatcaat tatctggttt gaaaaagaag
    85861 cgaaagaact tcttcctgaa ggatttaccc acccaggttc tcctaatggc gaatttacaa
    85921 aagaaaaaga tatcatggat gtctggtttg attctggttc ttctcatgaa gctgttttac
    85981 gtcagcgacc agaattatca ttcccagcag atatgtattt agaaggatca gaccaatatc
    86041 gtggctggtt caactcaagt atcacgacta gtgtggctat caatggtgta gcaccataca
    86101 aatcagttat ttctcaagga tttgctttag atggtgaagg ccgtaagatg agtaaatctt
    86161 taggaaacat cattgcacca gaaaaagtga tcaaacaatt tggtgcagat atccttcgct
    86221 tatgggtatc tagtgtagat tatgaagcag atgttcgtgt ttctatggat atcttaagtc
    86281 aagtggcaga agtatatcgt aaaatcagaa atacgatgcg tttcttatta gcgaatacag
    86341 aagatttcga tcctaaaaaa gatactgttt cgtacaatga tttacgttct gtagacaaat
    86401 atatgaccgt tcgattgaac caagtaatca aagaaatccg agaagaaggg tacgacaaat
    86461 ataacttcat gcatatttac cgtacagtta tgaacttctt gacggttgat ctatcgtcat
    86521 tctacctgga ttttgctaaa gatgtagttt atattgaagc agaagatgat tatcaacgtc
    86581 gttgcatgca aactgttttc tatcaaacag ctgtagcttt gacaaaatta ttgactccta
    86641 tcattccgca tacagcagaa gaaatctggt cattcctgaa agaagaagag gagtacgttc
    86701 agttaagcga gtttcctggt tatgaagagt ttgcaaacca agaagaattg atggatacct
    86761 ggactgcttt catggacttc cgtgataatg tcttaaaagc attggaagaa gcaagaaaca
    86821 gcaaattgat tggtaaatct ttggaagcaa aactaactgt ttatccaaaa caacaagttc
    86881 gtgaaatgtt aaaagctttg gatgcagata ttgcacaatt attgatcgtt tcaccagact
    86941 actttgaagt tatggcagca gatgaacaag tgccagataa cgctatggta tttgatgatg
    87001 ttgctattac agtggaaaaa gcagacgggg aaacttgcga tcgctgtcgc caagttcgta
    87061 aagatgtagg agtagatgaa aaacttcctc atctatgcgg tcgttgtgca aaaatcgttg
    87121 aagacttcta tcctgaagct gtagctgaag gattcgaaga aaagtaaaag ggaaaagggg
    87181 gtgtgacatg ttctttgtca cacccccttt tgttatcaat cgtttaattt tccacgttca
    87241 cgataccagt tatctggttg ccagatccat tcgaatccgt ctttttcaag tagttcgaaa
    87301 gctgctttag gtcccattga tccagctttg tagttgggaa aatccggttg tgtttggtcc
    87361 caagcttttc gaatgacatc cacgatatgc caagattgag cgacttcttc ccaatgagag
    87421 aagttcgttc catcaccatt caacgcatct aatagtaatt tttcgtaagc ttctgggcta
    87481 ttttcaacca tttctgcact atttctaaat tccaatttga ttggttccgt ttcaaatcct
    87541 tgccctactt ctttcccatt caaggataaa gaaaatcctt cagttggctg aatatagatc
    87601 gttagaacat taggtggaag tgtcgtatcg tcacatggtt catcgacaga tgttttgaat
    87661 acattaacag gaacttgttt gaatacgatg ttgatccgag tccctttttc agtcaaacgt
    87721 ttgcctgtac gtacatagaa aggaacacct gaccaacgga agttatcgat agtgaatttg
    87781 cctgcgacga atgtttcagt ctctgattga gcatccactt tgtcttcttc gcgatagccc
    87841 ttgaattgta caccatccaa ttcaccttga gcatattgcc cgcgaacgaa gttttctaac
    87901 acttcttctt cgttataaat gcggatagct ttcaatgctt tgatcttttc tgtacggatt
    87961 tctttttcgg aaaatgctac tggcggttcc attgcaagta atgctacaac ctgtaaaata
    88021 tgattttgca ccatgtcttt aagtgcgcca ctatgatcgt agtagccccc gcgatcttct
    88081 acaccgaggt tttcgccaaa agtaatttgg atattgtcga tatagcgatt gttccattga
    88141 gcctcaaaaa tattattggc aaaacggata gctgagatgt tttggatcat ttcttttcct
    88201 aaataatgat caatcctgaa aatatcttgt tctgggaata cttctctgat ttgctggttc
    88261 aattcaaaag cagactggta atcagaaccg aacggttttt ctatgatcaa acgattgaag
    88321 ccattttttg tcataatatt ttgtgatttc aaatgagaaa cgatcgttcc aaaaaattga
    88381 ggtgccatag ccaaataata gacacggtta ccgtttaatt gatagtgtcc atctaatcgg
    88441 tcagacaatt cttttaatgt ctgataatgc gttgaatcat ttacatcatg ggattgatag
    88501 taaaaatggc ttgcaaaggt agttgcttct tcatctgaag gattcaatgc acggattgtt
    88561 tctttgacaa cttcacggta gtgctcatcg ctccagggac ggcgagcggt tccgattaca
    88621 gcaaaatgct cgcctaaatt tccttttctg taaaggcgga acaaagacgg gtacaattta
    88681 cgctgggcta ggtcacctgt accgccgaat atcgtaaaca atacattttt ttcattagac
    88741 atgtggatac actcctttat attttatcga caaaaatcgt gctggccgcc ttaaagctga
    88801 cggggatttt ctttctatca ttttggatag tgatcggtcc ttcataagct gcgatgtcaa
    88861 cgatctcata ttcttcgttg atattcaggt caatagtgac taagtaatcc aacaattctt
    88921 tttcatcaag cactcgtgcg atacgcactt ttgtaccgac aggataggac tccaatgttt
    88981 gtcggcgttc ttcatggaca acttcctctt ttttaggaat cgatcctcca tgaggacaat
    89041 gttcaggatg attcaaatag ttttctaaat gatctgcaag atgttccgaa gtcacatgtt
    89101 ccaatacttc agcatcatca tggacttcat tccagctata gtctaaatgt tcaactaaaa
    89161 aaacttccca taagcgatgt ttgcgaatca gactgctagc tttttgcagt ccgcttgcag
    89221 tcaattgtac accttgataa ggggaatgtt ctacaagctc ttctttcaca agtttagaaa
    89281 tcatttcact gacagatgca gctgagacat ctagactaga tacgatttgt ttattgttga
    89341 ctttcgttgt atctccacct agttctaaaa tgattttcaa ataatcttct cgatttggtg
    89401 tcatattcga tcatcctttc atggtaaaaa accatcatta gtaagtgtaa caaaaaaaac
    89461 gttaattgac tatttttcta aataaaaaac aaaggatttt tagacgtttt tacaacaaaa
    89521 aacgaaagat gatagagaag actcattctt tcgtttataa aggatatata tatacattgg
    89581 gagggatcta tttttcagat aacaacttat atgaagtgtc tgcagattcg tcataaagag
    89641 tttgttcggg ataagaaaag ataagataaa aaccatactg atcacgtacg actaaacgat
    89701 attcatctat ttctaccaca gatgtgccaa gagactgacc gttgatcaaa aacgtgtaat
    89761 cagatgtaat ctgtaattgt tgtttttgct ggctatcact aaagaaccat gtaccttgat
    89821 actttgagag tccttctgac ttttccgctt gttttttctt tttcaagtaa ataaaacttg
    89881 agagaacggt aatgagtgaa ccaagaaaat agatcatttt ttttatcacc aaatcactcc
    89941 tttatgagaa aataatcgca tagacatatt ctttcttctt tatttcattg taccgaataa
    90001 aagctaaaat ttctctaaga ctttcgactg gtttttttac tgtttttcta caaattatga
    90061 taggatacat agtaggaaga aaggtgacat aatttgaagt atcagcaagt ggaaatagct
    90121 ttttttattg accgcccgaa tcggtttata gcacattgta tgaaaaaaga cggagaaata
    90181 attattgcgc atgtcaaaaa tacaggtaga ggaaaagagg tcctgatacc aggcgctgaa
    90241 gtggctattg tatttgctcc tggacctaaa cggaaaaccg cctatgatct gattgctgta
    90301 aaaaagaagg gtgactggtt taatatcgat agtcaattgc ctaatcggct ggcaattgat
    90361 ggtattttag acggcacgat ccttttacca ggattaactg gtccatttac ggtatacaag
    90421 cgtgaagtaa cttatcttca ctccaaattt gatatttatt tagagacggt ggtcggacaa
    90481 aaagcatttg ttgaagtcaa aggtatgaca cttgaaaata aagcgattgg tgcttttcct
    90541 gatgcgccca cactcagagg attgaaacat ataggagaat taacgtatgc cgcacaagat
    90601 ggttatgccg cttatgtctt gtttattgct cagttcgaac atcttcatct ggcaacgatc
    90661 catgaagaaa tgcaaccagc actagctgat atggttcgac atgcgcagca aagcggagtt
    90721 caaatcctcg cctataattg tcaagtatcg gcagatgagg taactgtcaa gcaggccatt
    90781 ccatttgatg tagatatccc ttttgaagat cctatacaat agaaagaagg taaaataatg
    90841 atcaacttag gtattatcgg aacaaattgg attactcatc aatttgtcca agcagcttta
    90901 agcacaaaaa aatatgaatt gacagcggta tattctagac atttggaaaa agcacaggaa
    90961 tttggttctc attatgaagg ggatatcgaa ttcgcaacag atttgaagac ttttttcaat
    91021 attactcata tggacaccat ttatattgct tctccgaata gtcttcactt tagtcaagct
    91081 aagcaagcaa ttttggcagg aaaaaatgtg atagttgaaa agccagcttt ttcgacgcca
    91141 aaagaaatgg atgaaatcat ccatttggca aatgaacaca atgtgtattt ttttgaagca
    91201 gcaagaaata tccatgaatc aggttttaaa aagattgccg attacttacc attaaaagac
    91261 gagataatcg gtgcaaattt cagctatatg aaatattctt cgcgctatga ccaagtatta
    91321 gatggagaag aaccgaatat cttctcgact catttctcag gtggtgcatt gatggattta
    91381 ggtgtttacc ttgtatatgc agctgttgct tggtttggaa tgccaaaaga agtccattat
    91441 ttcccaagaa agatttctac tggtgtagat ggtcttggat gggggatctt acgttatgag
    91501 aattttgatg tatcgatcca gccaggtaaa atcggtgatt cttatttacc gtcagagatt
    91561 tattttgact caggaacgct agtgatgaat ggagtcaatg caattgaaca agcacaattt
    91621 catgatcgcg cgcataaaga aatcattgat ttggcggtaa cagctgcaga aaatccaatg
    91681 gcagatgaag cagaggattt tgccaatgtc atggctcatc cgaaagatcc agcttgggga
    91741 ttgttgtatg aagagtggat cgagctagct agaaatgtga accaagtgat ttatgattta
    91801 agaaaaaatg gcggcatcaa attcgatgca gataatgagg aagattttta atgaacgaat
    91861 ttgtacaagt atttcccgaa aattggcaga aaaaatatga ggataaaggg tttatctcac
    91921 cttctcttat tcaacgagca gtttttcagc cactaagtaa taaacaaagt atagtagcgg
    91981 tttctcctac aggttcaggt aaaacactgg cttatctttg gcctctacta ctaaacgtgg
    92041 aaccaggtga agcaagtgcg ttggtcattt ttgcttcttc tcaagaacta gctattcaag
    92101 tagcagatgt tgcacgtgaa tggggaaaag acaaagagtt gaaagtccaa tctttggtgg
    92161 gcggagccaa cgtcaaacgt cagattgagg gtttgaagga aaaaccagaa atcttgattg
    92221 gtacaccagg aagaatattg gaattgatga aagcaaaaaa aataaaagca catcaagtga
    92281 aaacaatggt atttgatgaa gcagaccagc tatttgacgc tgggaacagc caaatcattg
    92341 atcaaattct tcatcaagct ccaacagagt atcagctagc ttttttttca gcgacagcag
    92401 atcgttccct tgaaagcatt gaaaaaatca ctgggaaaac gtttgagaca attgacgtga
    92461 cagcagagga cgattctcga aaaggactga gacattactt tttacgtgta ccgatacgaa
    92521 agaaagatga ctacttgaga cgtctcacgc atatagaaga ttttcatggt ctgattttct
    92581 ttaatcaact gactgaatta ggcgtaatgg aagaaaaatt gctgtataga ggtgtaccgg
    92641 taggaagtct ggcctctgat cagaacaagc tactccgaaa agcagcgcta gaacaattca
    92701 aaaagaaaat ggtcagagca cttttgacca cagatgtggc ggctagaggt ttagatatta
    92761 caggtcttcc ttatgtagtg aatgcagaag ttccgttaag tgaagaagct tatttgcatc
    92821 gttcgggaag aactggtcga atgggtaatg aaggaatagt aattacactc gtacaagata
    92881 ataatctaag agatttaaaa cggcttttac gtccatcaaa tataacactt gaagagattt
    92941 ttttatacgg aggtaagctg caaactgagc agccaaccaa agaagatgca gctcctgcag
    93001 taaataaaaa gtatgcgaaa actttttctg gaaaggagca gaacccagaa tataacaaag
    93061 aaaaaataag aaaaaaagtt aaaaataaat caaaaaaaga gagaaataaa ggagcaaggc
    93121 gcaaataagc gcctttttat gctatgatta tgaaaacggg aacaaaattg gttgccagag
    93181 gaggggttat gtgaaagaac gtcaagaaga gatcttgcag ctattgacag aaaaccaaca
    93241 aggattgaca gcaagtgatg tagctgagcg cttaactatc gatcgaagca atgcaagccg
    93301 atatttaagt gaactataca aggcccatca tattgtaaaa accgctggcc gaccagtcgt
    93361 ttactctttg ccaacggaaa agtcaaaatc tgatgaagtg catgtcgact cttctacgca
    93421 agtgactttt gaaacactcg ttggagaaaa tgattcttta aaagtcagca tacagcaggc
    93481 aaaagctgcg attctttatc caccgcgggg gttgcatacg attatttttg gagaaactgg
    93541 tacagggaag tcgatgtttg ctgaatgtat gtatcatttt gcaatcgatt cagagatgct
    93601 ttctgcagat gcgccatttg tttctttcaa ttgtgcggat tatgctcaaa atccgcaact
    93661 gctctttgga cacatctttg ggatcaaaaa aggggcttac acaggagcag ctcaagatag
    93721 tcccggtcta atagccaaag cagacggcgg tatattgttt ttagatgaga tccaccgttt
    93781 accgccggaa gggcaggaaa tgctgtttac tttcattgat aaaggtattt accgtccgct
    93841 tggagaaagt gcacaagtac atgaagcatc tgttcagatc attggggcga cgactgaatc
    93901 ttcagaaagt ttcttatcta cgttcaatcg acggattcct atggcaatca ccttgccgaa
    93961 tcttgcttca cgttcactgg atgagcgtta tgaaatcatc tctcttttta tcaaacaaga
    94021 agcgaatcgt ttgaatcaac gtatcgatat cgaaaaagaa gcgattcttg ccttcatgct
    94081 ttatgatgcg gaaggaaata tcggtcaagt gaaacgagac ttgaaactag tctgtgcaaa
    94141 atctttttta cattaccgta ctcatcatga gaaaaagcta atcattcgaa aagaagaact
    94201 ttcccttcaa gtacaaaaag gattactgaa aatcaaggaa ataccggaac gtctcgaccg
    94261 attcatggac agcaaaagtc agtatttgac atttgaacca ggatttgctg atgttgtctg
    94321 gtcacaagat ccagaacgaa acatgcaggt ctataacgat attgaggaaa aagtcacttc
    94381 tctttccgaa accggtgtgg agaacattga tctagagacc ctcatttcaa aagatgtgga
    94441 tgcttatttc cagacatatg tcaaagaatt gactaaaagt tcgattccca aagatctttt
    94501 gccagatgaa atctggcggt taaccaatca attgtatgat gtggctgaaa aacggttaga
    94561 ccgtatttat aatgaaaaag ctcgttttgc ttttgctttg catctgcaaa gtacattgga
    94621 tcgtgtgaga gaaggtcata tgatcgtcca cccagatttg aataacgtac ggaaaaattt
    94681 taagagggaa ttccaggtag caatcgatct ttcaacgatc attgaagaag aagaacaagt
    94741 tgagattcct tttgatgaaa tcggctttat tagtatgttt ctttctatca atctaggaga
    94801 aagtgagcct gttaaagaaa acaaagtaga tgttctcata ttgatgcacg gcagacagac
    94861 tgcttcaagc atgttggaga tggcacaaga actattagga acaaaagtag ggaaagcttt
    94921 taatatgcca ctggttatgg aagttcaaac aatgtatgaa gaagtgttac aatatgtcaa
    94981 aaactatcaa gagcatttga cgaatggcct actcttgtta acagatatgg gatcattaaa
    95041 caccttcagt gatttgattt atgaagagac tggcattcgg acgaaagcta tcacgatgac
    95101 aagtaccatg attgtgatag aagcgatacg gatggcaagt gttggaagaa gcttagaaga
    95161 tatttatcaa aatatccagc tttcctttga atcgatcgta cgtgatcagt tcaaagcgga
    95221 aaagtctgaa actttacagc gtaaaaaagc cgtaatcgtg acatgcttta ctggtgaagg
    95281 agtagctgcc aaattatatc agcgtattat gccagtgatt aatcaaaata aggtagaact
    95341 gatccagatg cagtttttag aacgtgaatc attcaaaaaa cacatcgatg ccttattgga
    95401 agaatatgag atcaaagcaa ttgcgggaac agttgatgtt gattatcaaa atatcccatt
    95461 tttttcagcc tatgacgttt ttgatgatga aaaactaaat attttaaagc gtatagctga
    95521 tgatgagatc ccgacagcta aaattgtcga ttcacttgct ggatcattaa gcgatgtctt
    95581 ttctattgat gaactgatcc gttcattgca aaagacagtt cagcagatcc agaccgattt
    95641 gtcgattatc gtggaacctg gagtagatac tggaatcgtt atccatttag ccttcatgtt
    95701 agacggattg atcaaacaag aaagccatcg gcaatttgag aaactgtcta ttttctcaaa
    95761 aatttatcgt ttggaaatgg atatcgttcg ggcaaacttg atggcactag aacgaaaata
    95821 tcaggtaaaa attccagaag atgatatcgc atttcttaca caaatgtttc ttgaaaataa
    95881 aataaaaaaa tgaacacact aatcatactg tttacacact aaacatctgt gtgcgcacta
    95941 taaaaaagag ctaaaaagca tgaaaacgct atttcagctc ttttttttat tggcatgctt
    96001 cttgcgttat ataaaagtgt aaaaaaataa aaggagtgtt aaaaatggca aaaaaaacaa
    96061 tcatgttagt ttgttcagca gggatgagta cgagtttatt ggtcacaaaa atgcaaaaag
    96121 cagcagaaga aaaagggatg gaagccgata tttttgcggt ctctgcctca gatgcagata
    96181 ataatttaga atctaagaat gtcgatgtat tattattagg tcctcaagta cgtttcatga
    96241 aagctcaatt tgaacaaaaa cttgcgccta aaggtattcc attagatgtc atcaatatgc
    96301 aagactatgg catgatgaac ggagaaaaag ttcttgcaca agcagaaaat ctaatgaaat
    96361 aaatcaagta tgcagttggg ggtataacaa cgtggaagat caacaaaatc tagaagccat
    96421 catggggtta atcatgtttg gcggtaatgc aaagagcgat gcaatggaag cgattgcggc
    96481 tgctaaaatc ggtgattttg agttagcgga taggaaaatc gctgatgcag aagaatcact
    96541 tatacaagcg catcatgcac aaacagacat gttgacacaa gaagcacaag gaaatcatat
    96601 gcaagtgaca ttattgactg ttcacagtca agatcactta atgacatcta ttgcgtttac
    96661 tgatttagca aaagaaatca tcgaacttta tcgtcgcgta tacagcgaat aaatacaact
    96721 tcttttttga acggaagaag aaaaattttt gaacggattt tttattttgt tataaggaga
    96781 actctcttta tataaaaaac ttataggagg aagtaaaaaa tgaacggact atcaagttgg
    96841 atggagaaat acatcctgcc ggttgccgca aaaatcggag cgcaaaaaca tttagttgct
    96901 ttgcgtgatg cttttatcgg cacactgccc gcaacaatgg cgggatcagt cgctgtaatg
    96961 atcaatgcaa ttattcgtga tttaccgcaa cagtttaaca gtacctatgc tgacagttta
    97021 gcagcagaca aaggaatctt tgccgtattg aatgtcatca tcggaatcaa tggttttgtc
    97081 tggaacggta cactagcaat cgccggattg atctttgcct tttcttgggg gtataatctt
    97141 gcccgagctt atggagtaaa tgatctagct ggggggatcg ttggtttagc taccttgatt
    97201 caaggtattt cctttggtcc agcattatca gcagcattag atgtgaatgt tccttcaaat
    97261 gtcgcagatg caatcaatag ttctgagatt ggtgcaacga ttgcagatgg cacactgtca
    97321 gttggattat ggggttggct aaaactagat catttgaatg gaaacgctta ttttacagtt
    97381 atgatcatgg gagctatttc agtcattatt ttttgtaaat tgatgcttgc caacattacg
    97441 atcaaaatgc ctgattcagt accaccagca gtatctaaag catttgcagc cattttacca
    97501 gcgacgatcg ctttatacgt cattgctatt atcaattata tcgtaggtcg tgtagctgat
    97561 gggcaactga tcattgattt ggtgcaaaaa tatattgcag agccattcct aggattatct
    97621 caagggttgg gagcagtatt gatcgtcaca gtatttgttc aagtattctg gttctttggt
    97681 attcatggac caaatgtatt ggcaccagtg ctagagggaa tctggggaca agcacaacta
    97741 atcaatattg atattttcca aaaaggttat aacggaaaaa ctggtacacc agccgtgtta
    97801 gatgcaatcg aagatggaaa agcttatatg tgggtccgcg gatcgttcga tgcttttgca
    97861 tggtttggcg gatcaggcgg aacgatcgtc ttactgatag ctatcttgat tttctctaaa
    97921 cgtgcagatt atctaacagt agctaaatta tccattgggc ctggtatctt caatatcaac
    97981 gaaccagtga tgtttggtct gccaatcgta ttgaatgcaa tcatgtttat cccattcttg
    98041 attgctccag tagtagcaac agcaattgga tggacagcta cttatcttgg cctagtcgct
    98101 ccggtatcgc agcaagtcac atgggtagta ccgccgttct tattgtcatt cttagcaaca
    98161 ggagcggatt ggcgggcacc gatcgtaaca ttagtatgta tggtcgtgac tttcttgatt
    98221 tgggcaccat ttgtcatctc tgcaaacaaa atggaccctg ccgcaggcga acaacaataa
    98281 gatgaacgtg aggagcggga cgctttaagt ttcgttcctc ataatgaatt ttcgaggagg
    98341 aactgaaatg attacaattg ccagtgcaat gcaacaagat gaaatcaaag aattgttaga
    98401 aggatacaat gaagaaggaa tgacttttac atttaaagaa aaacaaggaa tcaagctgtt
    98461 ctttgaaaca agtgcagaag atccggaagt tgcggctaaa aaagcaaagg aactaatcaa
    98521 agcacagcct tggggaacag tcctttattt tcaatcaaca gctgtttaga aaatggttta
    98581 aaaagatagg atggttgaga tgataaaaaa cggtttattt ttatgtagta ttggttcttt
    98641 gatcgtgttg tatagtttga atccacattc gcaaaggtat gatttgtata gtatggtcac
    98701 aggtcttttt ctaacaggat taggaatcta tttcttcatg aaggggaaaa aaagagaaga
    98761 agcaaaggag aaaaataact aatggaaatc acgaaaaaat taaatattcc agcatcgttt
    98821 ttttacgata aagttatgga ctctgtcctt ttcgatgtac gtaaagcgac cggcaaatca
    98881 gtaactagaa aacagctgaa aaatttggag tacgtcaaac aattctcgca aaataaccgg
    98941 gctcgcataa aaatcgaaga agtcattgaa aaccagtctt ataagtttcg tacatcgaca
    99001 acgaagaatg aattcgtcgt tcattatcaa atcaaaccat tggacgattc gacctgtgag
    99061 gttcattaca cagaaaaaat ggagtcttat ggcatgcttc aaaagttgaa tgatatgctt
    99121 ttaggaacaa tcttgatgta ttttaaacgg agacgtttca aaaaaatgct agatatgatc
    99181 gaagcaacgt attaaatcaa tgaaacatgg tactatttag ttattgaaaa atgaagtaag
    99241 ctagaaggaa tttttcatag gaagatagat ctttcctgcg aatcctcact agcttttttg
    99301 taggaagggg cagcaaaatg acaaacaaag taagagagct aggtgtatct tcgattgagg
    99361 aaatgtttga tttagccact tatgcattta atggggcaga tacacctgaa aggcgagaac
    99421 gttttcgtac acttgcggag aattcttgga attacggatt ttttgatgag gaggatcggc
    99481 taacaagtca agtcatggcg accccttttc cagtgaattt ttacggacaa gagtacctaa
    99541 tggccggcat tggctatgtt gcttcttatc cagaagcaag gggacaaggc gggatcaatc
    99601 agatcatgga aaagatcctt gaagattgcc gtgatcgtaa agtaagctta tcttatctgg
    99661 cgccattctc ctatcccttc tatcggcgct atggctatga acaatcattt gataaaatca
    99721 gttatcaact aaacagtcgt gatgtgccgt ttatcaaaaa aaaatctgga aaaataaagc
    99781 gtatggattt tgaagatgca aaagctgcaa tcagaagaat ttatgaacag atgccggaaa
    99841 accaacgagc tggtctgaag cgcgaagact ggtggtatac gtataaattc aagcaaaaag
    99901 gcaattacca gtttgctttg cattttgatg ataatgagga agcaacggga tatatcgttt
    99961 atcagcttgc aacgtcttcc tttattattg cggagtgggg atttttggat catgatgctt
   100021 tttgcagcct tgttcgcttc gtaagttctc acaatggtgc ttttgaaaca tttgaatatt
   100081 catgcggcta cggcggtaat gatcaaaatt atctattaga aaatccattt gcttctgtaa
   100141 tgatcacacc ttatatgatg gcgaggatcg tagatatttc tttgtttttg gaaaattatc
   100201 catttaaaaa gagacaagct gcatttgccc tacaaataga agaagatcat tatgcgaaat
   100261 ggaatgaagg aatcgtggaa gtaacaatcg aaaatggaaa aaatcatctc tcaaaagtag
   100321 aacaaacaaa actcccaatc gttcgaggaa gtatccaaca tatcacgcag ctattattag
   100381 ggtatcgtaa agtggaagag ttggtattcc aagaaagaat acaggtgaca aaagaatatg
   100441 aagaaacgtt aagctcaatc ttgccacagc aacagccaat tcttaatgac tatttttgat
   100501 aaaaaacggg tgatgacaat atttttgtca ccaccctttt gtttttcatt tggcatttga
   100561 aggaaaatgt taaagtaaat cctctttttt cgtaaatttg aattttttgt tttcttttgt
   100621 tttagggttt tcttttttgt ggtcttggat tgcttctttc gtaatctttt cccgattttt
   100681 tttagatgaa tctaagcgtt ctgcgtatac tttttctttt ggcttatcat tcattggaaa
   100741 ggacctccct acattatttc attctacctc tagtttatca tctgttaagg actagtggaa
   100801 acgttttgcc taacttataa gctgattttt ttagcagata agcttgcaag taaaaaacaa
   100861 acaagctaca attgatttat tgaaattgtt cctgtagctc agtaggatag agcaatcgcc
   100921 tcctaagcga taggtcgctg gttcaattcc agtcaggaac gtacaaataa cccctgtatc
   100981 ctttgcggta cagggggtta ttttgtataa gtagacattg tagtttctaa actgctatgt
   101041 cctaaacgtt tcgatacgct aagtatattt actccttgat aaagtaaaat agatgcatgc
   101101 gtatgtctta gtccatcaat aaacagtcga agagaaaata gggaaaaatt gattactaaa
   101161 gaaaaactta ctaggtcgac ggtgtagttg attttcatac ttcaatagat tcgaattaaa
   101221 cgatttcgat tggcttatac cagtcctatc caacgaaaga tatatcgttc ttaactctag
   101281 cggtataact tgaagcttta cgtaaagcca atgggaatga actggagatg actttttatt
   101341 atttttagta atagctgatg ggttaatact tacaactttc cttttcaaag taccatataa
   101401 aaaaacggtg gggttgttta gcgaaataaa ggatctaagc caatagctta gatcctttat
   101461 tctatgcttt atattatttt ttgcatgtgt gcgaaagtat actcaagccc aatcgccatt
   101521 tcggaaaatc ggtactgtag agccatcttc acggattccg tcgatatcca tcttgtcaga
   101581 tcctaccatg aaatctacgt gggtttgact tctattcaat ccagcttctg ctaattcttc
   101641 atcggacatt tctgtaccgc ctttcacact gaaggcataa gcagaaccta gtgctaagtg
   101701 attcgatgcg ttttcatcaa ataatgtatt gaaaaagatg atgccagatt gagaaatagg
   101761 tgaagggtca ggtacaagtg cgacttctcc taaacgacgc gcaccttcat ctgtatcaag
   101821 cagttttgct aagacatctt ctccttgttc ggcagaaaaa tcaacaactt ttccatcttt
   101881 aaaagtaaat ttcatacccg agatgatggt gccagcataa ctcagtggct ttgtacttga
   101941 aatgtaacca tctactcgat gactgtcagg tgctgtgaac acttcttccg tcggcatatt
   102001 tgccatgaat ttttctccac gagcattata gcttccagcg ccttcccaaa gatggttttt
   102061 aggcaatccg ataatgatat ctgtaccggg agcagtatag tgaagggcag aaaattgttc
   102121 tcgattcaat tcttctgctt tttttgctaa tttttcatca tgcttcttcc atgctaagac
   102181 agggtcttct tcatagacac gagtcgtttt gaaaatctga tcccataatg cctcaacttg
   102241 tttttcttct ggcagttctg ggaagacttt tgctgcccat tgtttaccag ccgcagccac
   102301 gacagtccaa ctgactttat ttgcttgagt tgccttgcgt aaattcatta gtgctttgcc
   102361 attcgctgat tgataagacg ctactcgatg gctatctaca ccagcaaatg catcgggatc
   102421 ggcagaaaca acgctgatcc tgcttgctcc tttttcaagc cattcatccg cttgatcgat
   102481 tttagattgt ggtacattct caatacgatc cgttgcagca tgtaaaagaa attctctttg
   102541 gatctggtcg tcagtccatt gaacgataac ctcagcggct cctagtttat aagcttcttg
   102601 tgtgatcaat cgagcaaggg gtgcctgttc cacgctgatc tgtaaaacaa ctgtgtgtcc
   102661 tttttctgtg gctacgccag tttctgcaat taagcgtgca tattttttta gaagttcatt
   102721 aaaatcagat aacataataa aaattcctcc tcaccatttt ttctggtaac tattagcatg
   102781 ataccattta attatgattc aaacaatgag gctttaaata aaaaaaggag gttatttttt
   102841 taaggttaag tttcatgata tatgttacta cttgctattt cagcttgtga agaaaatcaa
   102901 aaactaattt tgcatatcaa aaaaacatgt tacaatgaaa ttaatcaatg gtggaagcgt
   102961 tataaaaaaa ttaaggagat taaaaaatat ggcaagaaaa aagacgatta ctagagaaca
   103021 gattttggca gctgcttatg aagtagctgc caccgatgga tttacacgat ttactgcgag
   103081 aaacatcgcg aacaaaatga attgttccac gcaaccgatt tatcttgagt ttaaaaatat
   103141 ggatgacttg agacaagctc tttttgagca gatctatgat catttacaat atgaagtgtt
   103201 cactgttgag catacaggga atacgattat cgatcttgct tttaattata tccattttgc
   103261 aagaagagag aagaagttgt atcgttcact gtacttagaa gaatatggtg gaggaaaaga
   103321 gatgcagaat ttctcttata cttactttgt gaatacagtg aaacgcgatc cagcttatgt
   103381 gaatctttca gaagagcaga tcgaatcttt gtataatggg acatggatca tagctactgg
   103441 gatcgcagct ttgatgtctt caagcatcat ccatccaact gatgaacaga ttgaaaagat
   103501 gatccaagac agtatagatg ctatcttgaa attcaaagaa ccaatcgaaa tcgattgaat
   103561 atactcaaaa gttccacaaa tcagtaggta acaagagatg tgtcaaaata aggaaaagtt
   103621 cgtttttctt acagcgtatg catttttaac tcccgaagaa ctcttccatg aatgcaatga
   103681 ataaaagagc catacagatt gaataaatct ggatggctct tttattgcaa gtatattaaa
   103741 tttttgcgac tagttcttta gcgaaagctt ctaaattttc gatatcttct tcttcagcat
   103801 tcagatccac tttaacattt tcggctcctt ttgatgcgcc tgtttttgca aaagcagcat
   103861 cgaaatcatc caccgatttg cagaattcat cgtaaaaagt atcaccagat ccgcagacac
   103921 cgtaaatttt tcccaataga tccaattctt gcaagtcttc ataaaagtct acgatctcat
   103981 caggcagatc gccatcatcg tatgtataag tagctactac gcaaatatct gcctcctcga
   104041 attcatctgc atctacttgg gtacattcgt tgatttcgac ttcgatatcc agattttcaa
   104101 atgcttctgc aacaatatcg gcgatttctt cagtgtttcc tgtcatgcta gcatatacga
   104161 tttttgctaa agccatgatg tgcctcctcg tttataatta tttaacaatt atgaattata
   104221 ccaaaacatc attgaaaaga aaataaaacc taaagacacc tggggaatgt catttggcta
   104281 aatgtatgcc gctatgttaa aatgagaggg atttgtaaaa aggagacgaa aatatgatta
   104341 ctttaaaatc agcacgtgaa attgaaatga tggcagaatc tggagcttta ttagcagatg
   104401 tacataaaaa tctgcgtgat tttataaaac caggaatcac aagttgggat attgaggtat
   104461 ttgtcagaaa ctatatcgag agtcatggcg gaattgctgc gcaaattggc tttgaagggt
   104521 atgaatatgc gacttgtatc agtataaatg atgaaatttg tcatggattc cctcgtaaaa
   104581 agcctttgaa aaacggtgat ttggtaaaag tcgatatgtg tatcgatttg aaaggcggca
   104641 tttctgattc ttgttgggcc tatgtagttg gagaatctac acctgagatc gaccgattga
   104701 tggaagtaac gaagaaagct ttgtacatcg gtattgagca agcacaagta gggaatcgga
   104761 ttggtgacat cggtcatgct atccaaactt atgtagaagg cgaaaatcta gcgatcgttc
   104821 gtgattttat tggtcatggc gttggtccta cgattcatga agaaccagca gttcctcatt
   104881 acggagaagc tggaaaaggg ttgcggttga aagaaggcat ggttatcacg atcgaaccta
   104941 tggttaatac cggaacttgg aaaatgaaaa tggatcctaa cggctggact gcgtatacaa
   105001 gagacggcgg actttcttgc caatatgaac atacattggc tattactaaa gatggtccaa
   105061 gaattttgac atcacaagga gaagaaggca cttattgatc tatccagtca ataggtacgg
   105121 gagaggtaca tgaaagtttg gaacaaaata aaacaaaatg aaaatctgat gcgctttatt
   105181 gagacgactc aaaagcgcat cactgattca gaaatgagta cgacctctgt cgtcgttgct
   105241 tattatttac tcttgtcttt atttccattg attattgctt ttggtaatat tttaccgttt
   105301 ctccacatcg atcaagagac tgtactgacc tatttacgag aagtgattcc tgagaccgtt
   105361 taccagttta tcggtcctgc aatcaaagac cttttgacac aaagttcagg aggcttgtta
   105421 tccgtttctg cattagcggc tttatggtct gcaagtcaaa gtatcaatgc tttgcaaatt
   105481 gcgatgaata aagcgtatgg cgtagaaaat agaaaaaatt ttttgatcgt ccgtttcttc
   105541 tcactaattg tcattgtctt gttcatgatt gctattagtg gggtaactat cgttcttggg
   105601 ttgggacagc tgattttgga tgctattcag ccaatcgtac aaatacccgt gaactttatc
   105661 gatcagttcc aaactttgaa gtggccaatc acattggtag ctttatttgt catcatgttc
   105721 ttgatttatt taattgtccc taatgctcag ttgaagttga agacgatcat tccgggcacg
   105781 atcttcgcta ctgtcggctg gatgctgtta tcacaagtgt ttagtatcta tgctaggtat
   105841 tttgcaacga ggatcagcgg ttatcagatc ataggaagtt ttattgtact catgctttgg
   105901 cttaattttg ctgctacgat catcattttg ggcggaatca tcaatgcagt tgttcaagaa
   105961 tacgtgacgg gtcatcaaat caaaaaacgt cgagatccaa gccgaaaatg gttcaataaa
   106021 ttgaaaacta aattttctca taaaaaaaag tagaaacaag agttcttatt gtaaatttat
   106081 atcagtaatt tataataatt atgactctta attaaagtag gtgcccatat gcttgtttct
   106141 tttagtatgg ttataagatt ttttctgaca attttattat cactgatatt aacacctgta
   106201 ttcaaaatca tttcagtaca aacaggtatg gtcgacaaac caaatgaaag aagaataaat
   106261 aaagttccaa tgccttcagc gggcggactg cctatcttta tttcatttgt tatatcgact
   106321 ttattcttat tccgtaaaat tatccctcaa tcgtatattg taccgatctt gattgcttct
   106381 ttagtaatca tagtcacagg agtgctcgat gataaatatg agctgacacc taaacaaaaa
   106441 agtataggga tcttgattgc ttctcttatt atttattttg tagcagatat acgtattgat
   106501 tccttcacgc ttccttttat tggatatatc caactaggct ggcttagttt tcccgtaacg
   106561 attttttgga tcttcggtat cacgaatgcg gtcaatctga ttgacggttt ggatgggctg
   106621 gcggcaggga tttcattgat cggtttaatt acgataggaa tcattggata ttttttcctc
   106681 catgcttcta cagtgtatat tccagttgtt attttctgtc ttgtagcaag tattatcggt
   106741 ttttttcctt acaattttta tccggcaaag atttatttag gcgatacagg agctctattc
   106801 ctagggttta tgatggccgt cctttcacta caagggctga agaacgtgac ttttgtttca
   106861 tcgatttctt tgctgatcgt catgggagtg ccggtaacag acacgttttt tgctatcatt
   106921 aggagaaaag caaaccgtgt ttctttttct acagcagata aaaaacatct tcatcaccga
   106981 ctaatcagtt taggctttac tcataaaggt gctgtcctga cgatttatgc gatggctttg
   107041 atgttttctt tcacagctat ggtcatgaat tatactggaa gaatcggaac gatcgtattg
   107101 attattgcca tgttgtttgc tgcaatttta ttatttgaat tgattggtct gatcaatgag
   107161 aaacatcagt ttattttaaa aacattgcga tttttaggga ataaagagta ccgtacgcaa
   107221 gtaaaacaga aatatttgaa gaaatggaac cgtcatatag atgaataaca aattaaacca
   107281 gccgatcctt attagtattg tgacggcaaa cagtgataaa atttttcgga cactagataa
   107341 ttttatttca actgtaaaag atgaaacagc agtaaaaatc cgaatttttg acaacaattc
   107401 acttcccgag tacaaaaaaa gactaacaga gtataggaaa tatccctaca tcgatatcta
   107461 ttttcatgat gaaaattccg gatttggtta tggacataat cacaatctga tagctagcga
   107521 ttctcgctat ggcattattt gtaacccaga tattttagtg gatgaagaga cagttacagc
   107581 attggtcttg ttattgaaag aacatccgga atgtgcgatg ttagcaccta agattcttaa
   107641 tgaagatgga acaactcagc acctcatccg tgagcagctg gctgtttttg attacgtttt
   107701 gcgctttgtg cctttcaaat gggtcaaaaa aaagtttgac aaacgactgg ctgtgtttga
   107761 atgccgagga ttgcctactg atgctaatag ttatgtacgg atgatctcag gtagttttat
   107821 ggtagctgat attgataaat ttaaagagat aaaggggttt gatgagcgtt tcttcatgta
   107881 ctttgaagac aacgacttgt gtttgagttt tgaaaaagca ggttacaagt tattgtacac
   107941 acccttttat gcagttatcc atttttacgg gaagggtgcg catcgaaatg caaaactgtt
   108001 tcgcgtgttt ttgacttcta tgatgaaatt tttcaataag tgggggtggc gttttttctg
   108061 atgaaaccag aaatattagt agtcgttgtg ctttatcagc aactattcag tcaatctcca
   108121 gcttatggac cattaagcaa agcacttagc gaaaagcaaa ttcagcttat tatttacgat
   108181 aatagtcctc aaaggcagca aaatgagctg tttgaaaaag aaaatatcgt ttatcatcat
   108241 gatccgcaaa atcctggttt agcggtggct tataattttg cgttgtctca agcagatgaa
   108301 ggaacacgtt gtctcgtcac tctggatcag gatacgaggc tagtcgacga ttattttgaa
   108361 ctattgcgta aagtaacctt tacagatgaa tgtgttgctg cagttccgat gattttttct
   108421 gggagccgac aagtttctcc cctatattca gatcgttata tcaatcgtac agctcaagca
   108481 gtagaagttg aaggcccaac agctgaacga atcatggcaa tcaattcagg tgttgcctgg
   108541 tctgtcaaat ttctaaaaaa gattggcggg tttaatcctg ctttttcttt agattttttg
   108601 gatcattggc tgttttggaa agtggatcag ttggaaaaaa ctgtggagat ccttcctgta
   108661 cgaatggaac atgatctttc ggttttagac tatgaaaaag tttcaagtaa tcgataccgt
   108721 tctattttac aagcagaaaa tcgattttat catgaatatg atcaaaggca tttatccagt
   108781 caccgacgac aattattgtt aagaacagct aaacagttct tgaccgtcaa aaaccggcat
   108841 atctggcgga tgactttgcg ctcatatgtt aagaattgga aggtgtgaat tcgtgcgttc
   108901 ggtttgtata gctaccttta atggaggcag atatttaaga gagcagttag atagtatttt
   108961 atctcagctt tcagcagaag atgaagtagt catttctgat gatgggtcag tagatgatac
   109021 atgggatata ttaactgaat acgcagaaaa agataagcga atccagcttt tttcggggcc
   109081 tcgtcaagga ttgatcgcta attttggtta tgcgatccag cagacgaaag gagatctaat
   109141 ctttttggca gaccaagatg atgtttggtt ggagacaaaa gtgaaaaaga tcactgacta
   109201 ttttgagcag aatccggatc agctagtagt agtttctgat ttaatcattg tggacagcga
   109261 gctgcaagag atccatgatt cgtatttttc gtttcgaaat tccaaagaag gatggcttaa
   109321 taatgtgata agaagtcact atattggtgc tggtatggct ttccggtctt ctttaaaacc
   109381 cgatattttg ccgatcccgt cagaagtgcc gatgcatgat atgtggattg gtttgctggg
   109441 aggaaagaag actgggttcc taagagaacc gttaactctg taccgaagac atgatttaaa
   109501 cgcgagtgag attgaaacac attctaaatt acggcaaaaa atttcttggc gtgttcatct
   109561 gattacggcg ttgatcaaac gaaaatggtt taaacgttga taaaagatgt ttaaatcctt
   109621 aatatttcca tgacaaatat aacaattgat gagataataa caaagtgaaa tttgaaatca
   109681 aaaaggagag cagatatcca tgaagggaat cattcttgct gggggcagcg gaacacgtct
   109741 gtacccttta acaaaggcaa catcgaaaca attgatgccg atttatgaca aaccaatgat
   109801 ttattatcca atgtctactc tgatgttggc gggaatcaaa gaaattctga ttatttcaac
   109861 gccacaagat acaccacgtt tcaaggaatt atttggaaac ggaaatgatt taggactgca
   109921 gatcgaatat gcagtacaag aaagtccaga tggattggca caagcgttta tcattggaga
   109981 agaatttatt ggagatgata gtgtctgctt ggtattaggg gacaatattt actatggtgg
   110041 cggtctgtct aaaatgttgc aacgagcagc ttcaaaagat acaggcgcga cagtatttgg
   110101 ctatcatgtc aatgatccag aacgctttgg tgtagtagaa tttgatgaga atatgcaagc
   110161 tttgtcgatt gaagaaaagc ctgctcagcc aaaatcaaat tacgctgtaa ctggccttta
   110221 tttttatgac aatgaggtaa tttctatcgc taaaggtatc aaaccttcag aacgtggaga
   110281 actagagatt acggatgtca ataaagcata tttggataaa ggaaaactat ccgtagaact
   110341 tatgggacgc ggatttgctt ggcttgatac aggaacacat gaatcattgc ttgaagcatc
   110401 tacctttatc gaaacaattg aaaaacgtca gaacttaaaa gtggcttgtt tggaagaaat
   110461 tgcttaccgt atgggctaca tcgataagga acaattgctg gcccttgcac agccattgaa
   110521 aaaaaatcaa tacggtcaat atttattgaa tttggccgct gaataaggag tccttacaga
   110581 tgaaagtaac agatacaaaa ttaacagatg tcaaaatcgt agaaatggac gtctttggcg
   110641 atcatcgcgg tttcttcaca gaaagttatt caaaagcaaa atttgcagaa catggattga
   110701 aatatgactt tgttcaagac aaccattcac tatcagctga agctggtgtc cttcgggggc
   110761 ttcatttcca aaaaggagaa gctgcacaga caaaattgat tcgggtagtc actggagcag
   110821 ttttagatgt agtagtggat atccgcaaag gtagcccgac ttatggtcaa tgggacggat
   110881 ttatcttatc tgaacataat catcgacagt tgcttgtacc taaaggatat gcccatggat
   110941 tcgttacttt gacaccaaat gtgaacttcc tttataagtg tgataattac tataatgcag
   111001 aagcagatgg gggcatcgca tttgatgatc cagatttagc gattgattgg ccaattgatc
   111061 catcaagagc gattatgtcg gaaaaagatc accgtcatcc gacattaaaa gaatttgaag
   111121 cagaaaatcc atttgtttac ggagaaatct aagaattaag gagaaatttt cttatgaaaa
   111181 acatcattgt taccggaggt gccggattca tcggctctaa tttcgttcat tacgtggtaa
   111241 acaatcatcc agaagtgcat gtcactgttt tggataaact gacttatgca ggaaacaaag
   111301 agaacttggc cggacttcct agcgatcgcg tagaattggt cgtaggagat gttgcagatg
   111361 cagaattagt ggaccgtcta gtaaaagaag cggacgcagt tgttcattac gcggcagaat
   111421 cccataacga taattcatta aaagatcctt tcccatttgt acaaacgaat atcattggta
   111481 cgtatacact tttagaagct tgccgcaagt atgatgtacg ctatcatcac gtatctactg
   111541 acgaggttta cggcgatctt ccattacgtg aagacctgcc aggacatggg gaaggcgaag
   111601 gagaaaaatt cactgcggaa acaccatata atccttcaag tccatattct tctacaaaag
   111661 ctggttcaga tctgttagta aaagcatggg tgcgttcttt tggcttacgt gcaaccattt
   111721 caaactgttc aaacaactat ggtccttatc agcatattga aaaatttatt cctcgccaag
   111781 taacgagtat cttaagcggt attcgtccaa aattatatgg cgaaggaaag aatgttcgtg
   111841 actggatcca tacgaatgat cattcttcag cagtgtggct gattttgacg aaaggcagaa
   111901 ttggcgaaac ctatctgatc ggtgcagacg gtgaagaaga taataagacc gtgatggaat
   111961 tgatcttaga aatgatgggc caaccaaaag acgcatacga ccatgtaaac gaccgcgcag
   112021 ggcacgattt gcgttatgca atcgattcga caaaattgcg agaagagcta ggttggcagc
   112081 ctgagtttac gaacttccgt gatgggttgg ctgacacgat caaatggtac gaagaacatg
   112141 aagactggtg gaaaaaagaa aaagaggccg ttgaagcagc atatgccaaa aatggacaat
   112201 aatacagagg aaatttcact gtattaatgg aaaaacacat gtgaggcagg cgctcgcctg
   112261 tctctttttt aaaataaaaa tcaaggagcg gataaccata tgtttttatt aactggagga
   112321 aatggacaat taggtacaga attgcgccat ttattagatg aaaaaaatct gccatatgtt
   112381 tcaacagatg caaaagactt ggatatcaca gatgctgaga aaacaatggc ttatattaca
   112441 gaattaaaac ccgatgttat tttccattgc gcagcttaca cagcagtaga taaagcagaa
   112501 gaagaagcaa aagaattaga tgagaaaatc aatgtcgatg gcacaagaaa tgtagcactt
   112561 gcagcaaaag ctgtgaatgc acaattcgtc tatatcagta cagactatgt tttcgatgga
   112621 aaaaacaaaa atgtagaata cgaagtagac gaccaaacga atccactaaa tgaatatggt
   112681 cgtacgaaat tattaggcga aaaagcagta gaagagattt tagaagacta ttatatcata
   112741 cgaacttctt gggttttcgg gatctatgga cacaatttcg tcttcacgat gcaacgccta
   112801 gcggaaaccc gcgacaaact gactgtagta aatgaccagt ttgggcgacc aacttggaca
   112861 cgtactttgg cagaatttat ggtatatgtc atcgaagaaa aagcaccatt tggtatttat
   112921 catttgtcga acgaaaatag ctgttcttgg tatgagttcg caaaagaaat attgaaagat
   112981 acagatgtag aagttgcacc tgttacgtca gaagaatttc cgcaaaaagc aacacgtccg
   113041 cagtattcag tcatgagcct gaaaaagaca gaagcgttag gttttgtcat accaacttgg
   113101 caagaagcac ttgcgcaaat gctggaagct gccaaaaaaa tgaataagta atttcctttt
   113161 tatcaaatag aatttggagc atgatatgga aaaacaaaca agaagtgcac gacgaaaaga
   113221 aaagccaaaa gcgaatcagg cagattcgaa tttggtgctt gacctgtttc taaaactccg
   113281 ctatcttatt ggcgttttag tcatcgtggt catcgttact tttaatttaa acggaagttc
   113341 acttggtgtt tgggataaat atgtgtcgca aagagacgat gggaaaaaaa ctgatgtgat
   113401 ttttggacag aatcgggaag tgcggtcaga cgaatggctg gtgcagacac ctttttacct
   113461 ctcacaagct gaagaggact tttcgttggt gaacgaggat tattcattaa acggacaaaa
   113521 catgatcatt gcgtataatt ctcctgtaaa ggatattacg gttatcggaa aaccattcaa
   113581 ctggggattc tttttcctcg gaagagatcg aggactttcc ttttattggg cgatgaaatt
   113641 agtcgtcatg gtcttgcttg gattcgaagt actgatgata ttaacgaaga ggaaaaaagc
   113701 tctatcgcta atcggtgcat ttgcgctgac attttctcca gcagtccagt ggtggttcat
   113761 gcaacatgtg ggcgatttga ttttctttac tttgggactg atgattgctt tttatcatta
   113821 tttctacaat catgataaaa aatggctacg aacgttgatg atgcttttgg tggttatttt
   113881 cggtattggg tttattttag tgatttaccc agcccatcaa gtgatgctgg cgtacttgct
   113941 gattttttat tttgttggcc tgcttattta ctatagcaag aaagtttcat gggatctgtt
   114001 ggatgtagtg ttgattggag gggcagtttt atttattggc ggagtcatgg ttcatttctg
   114061 gctaacgtca aaagacgcat tgttagcttc tttgaatacc ttatatccag gaaaacgggt
   114121 aagtactgga ggaaagtgga cgattggcga atttttttct ttccttacaa attggaaaat
   114181 tccttttgaa gatatcaact attcgaataa ctctgaagtg gccctgttct atcacttttt
   114241 tccaactgta ttcctagcaa gtccatttgt gttatttggc aaaaaagata gcgaacaaaa
   114301 gttactggga cgtattttga tgattttttg tctgttttct atcttttgga ttaccgttgg
   114361 gttgccaaaa tggctagcag aaatcactct gctgtcttat gttcctccat taagagccat
   114421 ccttactttt agttttgcgg cttgtttatt atcgatctgg tttatcgctt atatttggag
   114481 agaagatgtt attcctattt ggtataaatt cattttgttg gcagtaaatg catggtctta
   114541 tttctatgcg attacgcatt cgaaaatgga taattatttt tcggattttg aaaccatcgc
   114601 agttgctgtg ttgggaacag tgattttagc ttttgcatta tttggctgga gacgcttgtt
   114661 ttacctcgct tttgtcgcct tagttatcgt atctggcttt ttcgtcaatc cagtcgtaga
   114721 aggtacagga gcgatttttg aaaaaacact ggcaaaagaa atccaaaaaa tcgatcaaca
   114781 agatcctggt aaagtttggt tgtctgaaga tgaattatat aacttcaccc caacattggg
   114841 agtgaaatcc ttcaatacag tacgctttta tccagatata gctgcatggg agaaaatcga
   114901 tccgaaagaa gaatacgaga agtattataa tcggtatgct catacaagag cttttatcgc
   114961 taaggaagat acaaagttca ttttagatcg tccagatatg ttttcggtca cgttgaattt
   115021 tgaagacgca gaaaaattag ggatcgacta tgtgatcagt aaacgaccat taactgatta
   115081 caaccaatta tatgaagcgc agttcacgca gctatatgga ccggataaag acggttatat
   115141 gatttttaaa gtagaatatc cggattatcc agatgtacca caaacacaaa ctcaggaaaa
   115201 tcagctagtc gtacagtaat aggctagctg agaataatcc tgaaatatta gtagaaagga
   115261 gagtgaagat aattcaagca atcgtacaaa aatttgtcaa caaaatgagt gtcggtttct
   115321 tgcttttgtt gctgacgcta gtcttttttg tttggacagc aggggtaggt tttccagcaa
   115381 ctttgtttat atataacaat agttcattac ttatcgcttt tagtgtggct attttgctca
   115441 ttttaaatgt ttataaagca aaaggcgtcg attttcttta tctaggtgtt gcgcttgccc
   115501 ttttcatttt cttttcttac tttcgggaat taagaaatga gtctttattt ggagacttac
   115561 tgtttccttt ggttatttta ttttgtttag taattaaatg gatcgagtgg acaaaatggg
   115621 atcgcggtat ttatctgatt gtctttacgg tcttcctcgg aatcacgtta tatcgtgtat
   115681 ttacagagat ccaagtacca gaaggacaaa gcatctgggc tccaggcaat tcgctttctg
   115741 atatttggat caatacgaat acaatcggaa gttctttgat gactcttgct ttattgatca
   115801 gtggatttgc cagttctttt gaacgatggt atgttcgaat catcggtggg atagcagtcg
   115861 ttgcagcctt tttaggtatt tgggtctgcc aatctagagg tgcattagtc gcgttgattg
   115921 tttttgcgat attggatctt ttacctaaat cgttgatgcg tgtcattcgt gcgccattta
   115981 ttgcctacac ggttacgatt cttttagctt tgccgatttc ttatctggcg gctgtctctg
   116041 aaaaagtcaa tttgtttacc gatcgagaag atatttggca taaattctat cagacgctag
   116101 gagaaaaatc tgaacaagtt ttactaggaa tgaaaacatt tatcttccag cgaggaaacc
   116161 aatttttagg aaaccacaat agttataatt ctattttgaa tatctatgga ttgataggat
   116221 ttgggattgc tgctttgctg cttatccttt ttattggtcg cttaacgttg aaagcagatt
   116281 tatccaacgg gcagatgact tttatctggg ccttttttgc ggtgatgatg cagtcattta
   116341 tggaggatac attgacttca tacccatggg ttccgattat ttatatcctg ttggctatgg
   116401 ccgttcatcg atatgatgaa ccagaagttg cggaggaatt ctatgttatg gaagaggcat
   116461 atgacgatat gacttatgat gatacatctt atgaggaaca accagatagc gctcattctt
   116521 ccaggatcca acgctatcat tgataggcac aaaatacgag agaggctgtg acaaccgtcg
   116581 cagccttttt tggtttattg caattgtcta gcatcttctg taaaatagaa acgttagaga
   116641 aaatcttgta tcatagaaaa ttaagaaaga aataagaacg cagctcggta ccaaaaatat
   116701 ggtaaaatgg cacttgactg taattcactg aaaatcgctt ggaggaacac aaatgtttaa
   116761 agatttgtat ttatttatga aggacattta tcagaacaga aaattattgc tacagttttc
   116821 gatcaatgac ttcaaatcaa gatatgcagg atcgttttta ggaatcttat gggcgtttat
   116881 caatccgcta tttactgtat tgatttattg gctggtattt ggtgttggac tgaaagcaca
   116941 aatgatcgat gcaaaatacc catttatcgt atatttagtt accgggatga ttccctggtt
   117001 cttcttctca gatgcatttg catcaactac ggttgttttt cgggaatata cttatttagt
   117061 aaagaaagtc gtattcaata ttcgtatctt gcccacgacg aaaatcctat ccaatctgta
   117121 cacccattta ttcttcattc tgattggcat agctgtcgcg cttataaatg gtatatatcc
   117181 ttcagcaatg tctttacagc tgatttatta tttattttgc atgtgtgctt tcttgacagg
   117241 attgacttgg ctgacggcat cgatccaacc atttttacct gatatcatgc aattaattac
   117301 gatcgtgctg cagttgatca tgtggacatt accgatatta tggagcccta atcagtttga
   117361 ttcaggtctt gtaagcatat tgaaattaaa tccgctgtac tatgttgttc aaggttatcg
   117421 tgagtcattt ttaagtgaag cttggttctg ggagcatgga acatatactt tgtatttctg
   117481 ggttttcacg ttagtaatgt tcgtaatcgg ctctgcagtg ttccgtagat tgaaaccaca
   117541 tttttcagat gtgctgtaag gagaatagct atgacagaat atgcaattag actaaaagat
   117601 atcacaaaat cttataatat gtacgcaaaa ccttcggatc gatttcgtga agccctcaat
   117661 ccttttaaga aatcctatca tgatgtattt tatgcgttga aaaacgtgaa tgtagaaata
   117721 aaaaaaggtg agatgatcgg tttcgtggga gaaaacggat cgggaaaatc tacaatgttg
   117781 aaaatcatta cgggagtact gacaccaaca tccggcacga tggaaatcga aggaaaaatc
   117841 tcagctttgc tagaactagg atcaggattc aatcctgagt attctggcta tgaaaatatc
   117901 tatttaaatg gaatggttct aggattttca agagaagaag tcgatgaaat ggtcgatgat
   117961 atcattgaat ttgctgacat cggagaccat atttatcaac ctgtaaaaac ttattcaagc
   118021 gggatgttcg ttcgtctagc ttttgctgta gcaatcaatg tgaatcccga tattttgatt
   118081 gtggatgaag cattggcagt aggcgatctt gaatttcaac taaaatgtat ggaaaaattt
   118141 acagaaatca agaactcggg aaagacgatt ttattcgttt ctcatgatat cagttcgatt
   118201 cgtcgttttt gtgatcgttc ctattggttg aaaaacggag aagtcgtcga atatggcgat
   118261 acgatggaag taacgacgaa ttatgaaaac tttttaaaga aaaaatcagt taaaacagtg
   118321 gatcggaata aaaaccaagt agaacggttc gccgctccag atatcgtcga cgtgacgaaa
   118381 gcggaattgt tggataaaga caaaaatccg gtggatatgc tggaacaagg agaaaaacta
   118441 tacgttaaag taacttatga agtaaaagat gatacaatca aaaatccggt tttaggaatt
   118501 gcgctacgaa cagtcgacaa tttttatgta tgcggactta atactctact agatggtatc
   118561 aagattcctt ggaaaaaagg agagaatatc ttttatctgg agtatgaaaa gttaggtttg
   118621 ttagctggtg aatattattt tgacgttgcg gtctttgaag aaaatgcaac ggttcctttg
   118681 gtatataaga caaagtacat gaacttgttt gtcagcggtt cttatatcgg agaaggcatc
   118741 gtggtcttag accataaatg ggaagaaggg actcatagca atgaaatatg attttgaaat
   118801 ggacctagat gaacaaagca gtgtgggaaa aattgccgca cagatcaaac caggaagtaa
   118861 agttttagag tttggtcccg gaaacggccg attaacgaaa catttaatcg gagcaaaaca
   118921 atgtgaagtg agcattgttg agttagataa ggaattgttc gattttgtca gcgaatttgc
   118981 tcaagatgga ttttatggtg atatcgaaag ttttgagtgg gcaaactatt atgctggaca
   119041 gacatttgat tacgtcttgt ttgcggatgt tttagaacat ttggtagatc ctggaaaaac
   119101 attgaagaaa gtcagagaat ttttgaatga agaaggagag atcctgatta cattccctaa
   119161 cttggcgcat aattctgtca tgatcgattt gttcaataac caattgccgt gggcttctta
   119221 tggtctccta gatgaaaccc ataattcttt ttatactcat gatggcttcc aaaaagtttt
   119281 tgaaaaagcg ggattattca ttaatattga agattatttg tacttagctg taggtgatac
   119341 agaattaaaa tcaacttatg aagaattgcc ggaagctgta cgctatgatt tcaaaatgcg
   119401 accatttggt gaagtctacc aatacttttt ctcattgatg aaacatccag tagcacaaag
   119461 ttccattgca gagccacaaa attctaatta tgtaaaagtg ttagaagtaa cccagcaaac
   119521 gaaacaagat gaaacaatcc aacaaatacc attcaataat ttcacagggg aaaatgagac
   119581 actaagtttc cccgtatcag aaacaacaga aagaatggtg tttcgcttcg cgcaacaacc
   119641 tagctttatc gaattcagcg cagaagctgc cggagaaaaa ctaacattta tcgattcgaa
   119701 tgctgtagtc aaaacagtaa atgattgtta tttgtttgat gggaaagaat taccagagtt
   119761 cgttctaacg gatatttctg ggaaagaagt gactatccat tgtcactatc gctttatcgg
   119821 ggaattgaca ccaacaatga aagaattgtt ggaaacgatt cgtcccatgg ctgaagtcac
   119881 aaagcagctt tctgagaaaa atgacgaatt agaaagagaa aatcatcatc ttcttgagga
   119941 aaatacaaga ctagaccata cgttaaaaac aaccacaaat cgctattgca cattgttgga
   120001 aagtgatgaa tggacaataa aacatagact aggacgacgt aagaaagaaa cttcgaaaaa
   120061 aatccaagaa aaagaacttt ctatatgtat tgacgagaaa atctgggatg cagaaacaaa
   120121 aatattaaaa atcatcggat ggggaatcgc taattcggat cgccagccct tgtcctataa
   120181 gttatcagct gaccaatcgc catttttcga agcaattcct gtttcacgag aagaagtcaa
   120241 tcaagcagaa cagctcgcaa aaggaacaga agcgggattt gagttacgaa tcctttgcga
   120301 gcaagaacgt tcattcttag tggaagcagt agctcaaaat ggccaatcat ggatcattga
   120361 aatgtaaata atgattaact tataaagata aggagtaaac gaatcgtgca aaaaaaagaa
   120421 attgccgtat ttatcgatca aatcactcgt gaacgggcca ctggcgattt gcttattgtg
   120481 ggctgggcaa ttgatgaagt gaccaaagaa atccctacga taaaggtaga aaaagaaaat
   120541 gtgattgctg aagcaacaca tgtcgtaaga ttggatatca atcatttata taatcttgat
   120601 gttaagaccc aaagcggttt caaggtacgt ttatcaggga aaatgagagg aaaagccatc
   120661 ttagacttcc aaacagcgag acatcaaaat ggcatagctg tgaaattaaa cggtaaatac
   120721 ccttatgatg acggcattga atcttcatgg gaaagaaaaa aaagattact gaaaaaaggg
   120781 ataaattatg caaggactca tggcgtaaaa aaagcgatcc gtcgggtcaa attggaattg
   120841 aagccaggat cgatcgacta tgcagagtgg atcagtcgtc atgagaaacc agatttgaaa
   120901 gagcaaagga aagaagcaca aggatttgcg taccgtccat tgatttccat acttatgcct
   120961 gtctataacg ttgaaatcaa atggctcgaa aaatgcattg attcggtact ggatcaaacg
   121021 tatgatcatt gggaactatg tatcagtgat gatgcttcta ctgatccagc tatccgaaag
   121081 tgtctggaat cctatcaagc aaaagatgat agaatcaaag tcgttttccg aaaagaaaat
   121141 ggacacatca gtctggcaac taattctgca ttggaaatag cagaaggaga atttatagca
   121201 ctcttagata atgatgatga attaccgcca tttgcattat atgaagtggc aaaagtgcta
   121261 aacgtacatc cagaactgga tgtgatctac agtgacgaag ataaaatcga tgcagatggc
   121321 aatcgctttg atccgcattt caaagcagac tggtcgcctg atacattgat gggaaataat
   121381 tatatctctc acttaggcgt atatcgaaca agcattgtta aagaacttgg cggtttccgt
   121441 aaaggctatg aaggttctca agattatgat ttggtccttc gtgtgacaga acaaatccct
   121501 gcagatcata tttaccacat tgaccgagtc ttgtatcatt ggcggacgat cccaggatca
   121561 actgcaagta atggcgaagc caaaagttat atctatgatt ccggagtgaa agcattgaca
   121621 gatgccctgt ctagaagaaa catcaaagga agcgtacgtc ctggcagaat ttctggattt
   121681 tatgagattg cttatgacgt cttgcaagag gatttggtca gtgtgatcat tccaacgaag
   121741 aatggctatg aagatctaaa aacttgtgtg gattcgatca tcgaaaaaac gtcttattca
   121801 aattatgaaa tcattattgc ggataacgga agtacagatc caaaaatgca agaattattt
   121861 gcagaataca agcatcagct gaaagaccgc tttatcgtag aattgattga cattccattc
   121921 aattattctc gaattaacaa tttagcagca gaaaaagcaa acggcaaata ctttttattc
   121981 ttgaataacg atacagaagt gattgagcca gattggatga cggtgatggt ttcttatgca
   122041 cagtttgatc gaatcggctg tgtaggagca aaactgtttt atccagatga tacaacgcag
   122101 catgcaggag tattattagg aatcggtggt gttgcagggc atgcattgaa taactatgat
   122161 cgaacacatt gtggttattt cggccgattg gtcattgacg tcaattactt agcagtcact
   122221 gcagcttgta tgatggtcaa ggcagctgac ttcaatgctg ttaacgggtt tgatgaaaca
   122281 ttagaagtag cattcaacga tgttgacttg tgcttgaaag tttatgaact gggtcgttac
   122341 aatgtctatg cacatcaagc agaattgtac catttcgaat ctaaatcaag aggttatgaa
   122401 gatactccag aaaaacaaag acgttttgct ggtgaaatca aaaaaatgca agataaatgg
   122461 ccagcatata ttgcgcatga tccattttac aatgataatt taacaaaaga cggaatcggc
   122521 gacttttcgt tgagaccaga ttaaactata ataaaaagct gttatgttgt gattcgctcg
   122581 cacataacgg ctttttatag ttttttgtca aaaaaaggca gaaggtgccg actcaaaaaa
   122641 ataagtcgag gttgggacag aagtgtttaa ctccgagaaa taagaagaaa ttcacgaaaa
   122701 ttgcttttca aatttttgtg aatttcggct tatttaagaa gaagttgctt ctgctcccac
   122761 cgtttattcg tttttaaaga atgagacaaa agtgtttctt acttttgtcc cacgctctta
   122821 ttcatgctca gagcgccaat agatacaaaa tcatagccaa agtttcacga accctagctg
   122881 tcaaagaatc gatattttat tttaatatag gctattttaa aataaatggt ttaaggtatt
   122941 cctctacaaa agccactcta tcaattatca tgggtttaaa ataagaagtg atggagataa
   123001 ccagattgtt cttagcgtca atataaagaa catttccact attaccaatt gctgaatata
   123061 tttcgttctc agaatctaat acccaccata aatagccata tctcatattt tgtaagtctt
   123121 ctaaagcact ggttctaggt ttgaacatct cgtttatcca catgggtggt acgatttgtt
   123181 ttcctttata catcccttta tttaggcata atacaccaat ttttgccaga tcacttgccg
   123241 ataaacacaa accatatcct gccgtcccga tatttagtgg atcacaaaac cagacattgg
   123301 ttttaggaga cttatccatt gtaaactgct tatgttcttc agctgtttca gcataataat
   123361 tgacatggtt tggtatccct ataggatcaa atagatactt attggcaaaa tcaacagttg
   123421 ttagcttact tgtctgatac aacaagccag ttaatatgtg taaacagaca gtttgatagt
   123481 tgaattcgcc ggttatacct tttcttcctc ccaataaatc tagtgatgtt tttgtccaat
   123541 cttcacttga acagactttt gtccatggat cgcctttaca tttgtagggt gctttcattg
   123601 ttagcaagtg cttgattgta atagcgtaaa tagttttctc cccgcgtttc actttatact
   123661 ctggaaaata atctaatact ttatcatcga tacttttgat taatccttgc tctaaagcaa
   123721 tgccaatcag caaagataca atacttttcg ttgctgacat gatatgacaa gtatcgtctt
   123781 ttttataacc attccattca tctgtataaa cttctacccc atccttatat gcgacaattt
   123841 gacatatatt tggttgtcga aaaccaataa aatcatgcaa ctcttgttta ttcataagat
   123901 attacctcac tttcttctat aagcataatg tcattaaaaa tcgatttcaa ttttttcgat
   123961 tcttcacgta atacaggtat tcttatggac tagacagcgc tttgggaaga tggattctct
   124021 gaaaaatata aaggttcact ttatgagtgg ataaaaaaag cagcaaatac ttcgacttct
   124081 ttaatttacc aacattagtg aaaatcgatt catctggaga tttgtaattt agagtccttg
   124141 tcttgattag aggtgtataa agctttttat tagacatttt taaattccat tacaatttgc
   124201 ttataaacta ggtttttaaa ggtgactatg gtaagataat agagatatta ttttgtgatt
   124261 aggagtgtat atatgaataa aaatggagaa tggagtgctg cacgacggat attcattata
   124321 ttaatggatg ttattgtata caatgcagca atatatagta gtttcttact gaagtttcgt
   124381 ggagagatcc cattacgaaa tttagaaact ttccaacact cagcaattat tatttcagtg
   124441 atttttattg cgttgaacat tttgttgggt gcatatgttt tttataatcg aatgatcagt
   124501 gacatcgtgt ttgtcacagt catcggtcag gtattgatga cactagggat catggtagtg
   124561 acttttgcgg gccgctggtt tgcttttcca cgtcttgtta tcttactttc atttgttctc
   124621 ggtacgcttt tcttaagtat ctatcgtatt ctcgtatata aactttattt acgcgtaagt
   124681 cgagataaaa aggtcgtcat tattgggtta gaaaaggatg tcgcaccagc aatccaaaat
   124741 tttggttcaa agaaaaacaa caaacataaa gtcgaagcag tcattcttga taactattat
   124801 gcaaacatca aaaatatgat taaagacatc gatattgtct atcttgcttc tcatatcgaa
   124861 gaaactgaaa aaatcaaaat ctatcaatta gttactaaaa atgaaaaaaa attgtttttg
   124921 aatacaacat ttgaaaactt gacattgatc aatccaaata ttatgaattt cgaagatgaa
   124981 agtattattg aagcatctgg atttagaatc caacctgaat atgaactatt caaacgtttg
   125041 tttgatatca tcgtttcact tgttttatta gttattgctt caccatttat gttattcaca
   125101 gcaattttag tcaaagtgac atcaccggga ccaatcatct ataaacaagt gcggatcaca
   125161 aaaaatcaaa aggaattttc aatctataaa ttccgctcga tgaccgccac agccgaagca
   125221 aaatctggac cagttttagc taagagcaat gatgcacgag taacgcctgt aggtaaattt
   125281 attcgagctg tccgctttga tgaattacca caaatcttca atgtgctgaa aggcgatatg
   125341 tcgatcgtag gaccacgtcc tgaacgttct ttcttcgtcg atcagttcaa tgcagaaaac
   125401 ccttattact acttacggca taatgttcgt gctggtatca ctgggtatgc acaagtctat
   125461 ggaaaatatg tctctgatta caacagtaaa ttacgttttg atttacttta tatcaaaaaa
   125521 tattcattga tgcttgattt aaagatcttg ctgcaaacca tcaagatcct atttgataaa
   125581 atgtcctctc aaggacttga agaagacgaa aatgtgatct cattggcaga aattgctttt
   125641 ccgaatgaaa aaatctttcg ctagttggga ggcgtaaaaa tgaacaatcc gaaattcagc
   125701 ataatcgtac cagtatacaa tgcagaagca actattggtc gaacgattga tattctaaaa
   125761 gaattggatt atgaagagta cgaagtcgtt ttagtaaatg atggatcaac agatcaaaca
   125821 caggaaatca ttcaagaact gattgaaaat gatgaacgtt ttgaattgat tactacgacc
   125881 aatcaagggc ccggattagc aagaaacgaa ggaataagac agtcaaaagg agaatatctc
   125941 ttattcttcg atgcagatga cacgccagta aaaacgatct tgtcagatta tgatcatttg
   126001 ctagaaaaga gtccaaaagc ggatcttgtt gttagttcgt ttgtttttcg tacaatggac
   126061 gagggaagaa tcgtttccga aaaagaaaac ttagttacag aatgtcacta tctaactcac
   126121 gaagagttta taaaagacat gtatcagtta atgaatgaac agttgatgta tgttgtatgg
   126181 aacaaatgct atcgtagcga tattctgaaa aataaccaaa ttctatttaa aacttataat
   126241 agttgtgagg atcgaatatt taacttacat tattatgaat actgtcatca agtgatcatg
   126301 aacccaaaag tagaatatat ttatgaattt gaaggtggga aggggatcac taatcaatac
   126361 cgacctaaca agttcgaaac atttaaagaa ttctattcat tagcaaatac tgtaacagac
   126421 gatattaata aagacggtat ggcggcgctt cttttaaaag ggacaacttc agttattttt
   126481 tctatttata gtacctctgc aagaacttca aaagagaaga aagacgaagc aaagcagata
   126541 ttgagtgatc cagtgatccg agaagccaag caaattgcta agacggattc ttcagcaaaa
   126601 aaaataacaa agttgcttta caatatgcca gctccactat ttttatctgc tgtaaaagtt
   126661 ggaagcttcg ttgaagtgaa aatgccaggg ttaatggctg ctttgaagcg agtatattag
   126721 ttaatggagg aaaatataat gaaaagaatt ttgcattttc agggacgaat gggtttaggt
   126781 ggagcagagt ctttcatgat gaatttatat cgtaaaattg atcgtacaaa ctatcaattt
   126841 gattttttga tttatgaaga ttatgcagat gttcaagact atcattcaga gattgaaaga
   126901 ttaggaggcc gtatttttgt tgtacctaat cctaagaaaa atatttttaa atatttaata
   126961 gaagtgaata agttactaaa aaaagaatct ttttctatag ttcacaatca ggtttatttt
   127021 ggcgggggaa ttaatttatg gttagccaaa aagaatggaa tcagacagag aattgctcat
   127081 agccatgcaa ctgaagatgg aaaatctcaa aatattgtaa tgaatgtatt gcgtaaattt
   127141 ttaaccaaat tgttgttaca aaatgcaaca gactaccttg ccgttagtca agaagctggt
   127201 gaaagtctgt ttcaaaacca tccctttgag atagttcata atggtattga tttagagctt
   127261 tatagtaaaa attcagaagc taaggttaat aagagaaaag aattggatat ctcaatgagt
   127321 acttttgttg tgggaaacat tggacgatta gagaaacaaa aaaatcaatc ttatttgtta
   127381 gagatttttt tagaaatact aaaaatgaat tctgattctt acttgatttt agtaggtgaa
   127441 gggtcactta gaaataaact gaaagccaaa gcagtagaat tggatataga gaaaagagta
   127501 aaatttttag gagaaagaaa cgatattccc gagttattat ctgtgatgga tgtttttgta
   127561 atgccttctc tttatgaagg tctgccaatg gttggattag aagcacaagc agcaaaatta
   127621 aaattagtct tagcagatac tatttccaaa gatacaaagt taacaaataa tgtggatttt
   127681 gtatcattag atgaaacacc acaagaatgg gcaaaaaaag taataaaaga gccgtatgct
   127741 aataaaataa cacaagaact aaagagttat gatgtttcgt acactactga acaaatggag
   127801 agtatatata gtagagatca ttaaaaatgt tcaagatatt ttaggagtga ttatttttga
   127861 aaatatgtta tttgatattg gcatacaatg atattggcaa cttaaagaga ttggtcaatc
   127921 gattgaacga aaatgcagtt tttattattc atatagataa aaaaactgat atagaaccat
   127981 ttattgaagc atttaaatca tatgataatg ttgaattttt atgtaataaa aaccgtgtta
   128041 aaatttattg gggtggtttt tccattgtac aagcagaaat caatttagta aagagagcat
   128101 tacaaaatga gaaatattta aaatatgtat tattatctgg tgctgattat ccaatcaaag
   128161 ataatgagta tatttataat tattttaaaa agaatagttc agtggagttt attcgtggaa
   128221 tagatctaga tcaaattaaa cataaagaat tttattataa acatatagat gtatatcaaa
   128281 aacatgatta tcctagaatt aataggaata acacaactgc atttaaaatt tttagagcta
   128341 taattaatcg ttgtctgagg atgattaaac ttcccccaaa aattagacac cataaatttg
   128401 atttgtatca tggttctcaa tggtgggctt tgtcgaaaga atgcctaaca gaattaatac
   128461 aaatgtatga acaaaatcaa caagactacc tgaattttaa aataggtatg tttgccccag
   128521 atgaaaaatt ttttcataca ctttttttta attcctcgtt caaaaataaa aatgttattg
   128581 gaggaccaga tttaccactt gaattgaaaa atattgaaga aactacttta caaacttcga
   128641 aattagcaaa tattcatatt attgatcctt ccatgaacaa atggtttgat gaaagggata
   128701 tagatcaaat aaaagaaagt gaaaaattat ttgtaagaaa agttaaatcg gattactctg
   128761 ataaactatt aaatagaatt gataaagaaa ttttgaaagt tggaaatata gatgaataga
   128821 atagtacctg ttttagagaa gagtttatta agtcgagatg cgtttcgagt aataaattta
   128881 atcatttgtc tgctttattt tatgcctagt acttttaatg taactaatat cccgatgaaa
   128941 atagcttttg cttggggtgg aattatagtt ttgtatgagg tttttataaa gaaaaatttt
   129001 tttaaaatgc gtttttcttg gctactgatt ggagcaatga ttagttttat cttaactata
   129061 atcattaaca gagaaaattt tttaattcca tctatttata attttggata tttgctgatt
   129121 actttgatag taatttttcc tgcagacttt tcacaaagtg aagaggaaaa aaagaaaaaa
   129181 atggtgtatt ttaataatgt tttcattatc ataatttttt tagctgcgct aatatctatc
   129241 tatcaattta tttttttgat ttcttatcat gtcccaacag gaacgccagg attacttgca
   129301 agacaaggtt ttattgaaca tcggttattt ggtgtctata caagtccaaa tgttggagct
   129361 atgtttggat atacttctat tattttaagt ttaattagtg tattaatttc aaaaataaca
   129421 aaaacgattc gtgtttttta tatgatgaat tttatcgtac aaatatttta tattacactt
   129481 tcttcgtcta gaggtgccca ggtagtaatt tgttcttttt tagtattatt tcttttgttg
   129541 tttgccaatt caaaatttag aaagagcatt agagaagtac tacctgtaaa aatcaaatgg
   129601 atggtaagca tattattagt tctattggta tatttttctt ttggtactaa ttatgctaag
   129661 gattttttag caacaattcc tgtatcgatt gatagaaaag tagaagttat agagtcaaat
   129721 gaatcagata aaactccact tccagaaaaa gaagtaatat tacagcattc tagtgataac
   129781 tccgaaattt cagctggtcg ttttacgatt tggaaagctg cgcttaaagt tataagacca
   129841 actttgttat ttggttatgg agagaccgat ttctatggga atagcgatgc aaaatttttg
   129901 aaaaaagtag atcttgatac tacagatgtg aatgaactaa aaagagcaca tggaaacatg
   129961 cataatggat ttcttcaagt acttgtatca agtggtataa ttgcttttat atgtatctat
   130021 gctttctatg tgttaaatat ctttagttta ctcaaaacat tttttagaag taaagtgaat
   130081 tatgcgtttc ttggtattgt tttaattttt atactatcta tttttataga tgaaatggtg
   130141 gaggctcatg tacttttcaa taagcgcgat gtcatttcaa ttgttttctg gtattatttg
   130201 ggaagcattt cttacttgta tttaaaaaag gagaattaat atgaagacag ctttcattgc
   130261 ttggactcct ttacatttga ttaatatatt aaatgtttat caaaattttt accgagaatc
   130321 aaaaggagat ttatttattt attctgaatt tgaaaacgct agaagatata gtgagcgtat
   130381 aaaagaatta gggctattta atgatgttgt ttttgtgaat caccggaaaa tcggttcttc
   130441 ctttaaacgt aaaattaact tgttaactaa ccataatcaa tttttctcgg atgatttatc
   130501 cgtttatgat gaaattttta tacaggggga aaattatttc acaaaaatac ttttctccaa
   130561 tgtttacaaa aaaaatccag atgtaaaact aaattatata gaagatggtt tgggtactta
   130621 tttgaagaag agtatatttt caacaagtaa aaacgttcaa agagttttta aaatattaaa
   130681 taaaaatagc atcttcaaaa aaaaaattga cgattatttt gtctattgtc cggaattgtt
   130741 actttatgat gtaaaaaata taaaaaaaat tcccaaaata gatgaaaaca gtaaattgat
   130801 gaaaaacata caatcaatct ttgggataga agaagttttt gtcggagata aatgtatctt
   130861 ttttgatcaa cctttattat ctgatggtta tggcatagat gaaacagata ttttcgctaa
   130921 agtaaatagt atattttctg gaaaaaaaga aactttaatt aagcttcatc ctagagcaag
   130981 gacaaatcgt tatggtgaaa aaaacatatt gaattccagt ttaccgtttg aagtcttgct
   131041 acttgaaaat gacttctctt caacactttt gattagtcct atatctacaa tttctttttc
   131101 accatctatg atgtttgaaa agacgatcaa ctctattatg ttggctaaat taattttaaa
   131161 tgaatataca gcattatctg aagataaaaa agaaacattg acaaatattg ttcttttctg
   131221 tgaacattat aaccgtctat cagaagaaaa aattttactc ccagaaaact gggcagaatt
   131281 aagaaagttg gtagagtatg ggattattta acacactttc caagtcaaaa ttagttaagt
   131341 ccagtttctg gtatactata gggaattttt ttattaaggg aatttcattc ataactattc
   131401 ctatttttgt aaatattact actcaagcag aatatggttt gattaataat ttcacttcat
   131461 ttgtatcaat tttttcgatt cttgttgggc taagcctaaa ttcggcaatt aataatgcaa
   131521 gttttgacta taaagatgac ataaatgaat ttatgtcatc ggtactttct ctagctacac
   131581 tttcgttaat tgtgttctta ttaataggga acatatattt tttgttttca aatgcttttt
   131641 ttgatatgaa ccaacttgta tttaatttga tgttattaca aagttttgct agttttgtaa
   131701 ttagttatat ccagtcttat tttacgatta actttaacca ttttaaatat ttgattttat
   131761 cttttatatc tacttttttg aatattgcaa tctctgtagc tttgatgcag acattatatt
   131821 ttgatgataa atatgttggc agagtagtcg gcggaacact gtcatttgtg ttgataggtt
   131881 tagtaattta tttaagaata atgataaagg gtgctcattt ttttgataaa aaacagtgga
   131941 gatacgcttt aaaaatctct ttgccgctaa tcccacattc cttagcaaat atagttttag
   132001 cacaattcga tagaattatg attaattctt atttaggtcc tgctgcgggg ggaatatatt
   132061 cctacattag taacataggt attattctaa gcgtaatatg gatgtctact aataacgcct
   132121 gggttccatg gttctatggt gagatgagta aaaataatga aaaaaaagtg aagaaagtta
   132181 gtaactatta tttagtagtc tttactttga ttacgatgat tgtaatggta gtttcgattg
   132241 atttagcaaa atttatggct ccgaaagact attattctgg gttatcttta gtggttccga
   132301 taaccttagg ctatttcttt cagtttttat atagtttacc agttaatgct gaattttatg
   132361 agaaaaaaac atcgtttatt gcgattggta cagtagcaag tgctatagtt aatatactat
   132421 taaacatgat tttcatacct aggttaggaa taattgctgc tggatacaca accgtattta
   132481 cgtatttttt actttttatt tttcattata atatcgctaa aaaaattacg aataaacaat
   132541 tatttgacac aaaaaaaata tgtatcataa taatttttgt atcaattttt tctttgatac
   132601 ttcatatttt aatcaatcaa atgttgattc gttacgtttt gacatcatta ttgataatta
   132661 gtttagttat atatttttta aagattaaga atcgaattat agatgactat aattcatagg
   132721 aggagatata agtgtttaaa ggaaaaacaa tattagtaac tggcggaact ggttccattg
   132781 gaagtgaaat tgttcgtcag cttttaaaat atgaaccaaa agctatcaga atttatagta
   132841 gaggtgaaga aaaacaattt tatatgcaac aagagctctc aaatcacact aatttgagat
   132901 ttttaattgg tgatatcaga gactctcaaa ggattgatta tgcatgtcgt gatgtggata
   132961 ttatttttca tgctgccgca atgaaacacg ttccagcaag tgaatataat cctatggaag
   133021 ctgtaaaaac aaatgtgatg ggaactcaaa atgtaataga tgcagcgatc aaaaataatg
   133081 taaaacattt tgtaggtatc agtacagata aagtagtgag tcctactaac accatgggtg
   133141 cgacaaagtt attgagtgaa aaattaataa tagctgccaa tcagtataaa ggaaaaacag
   133201 aaacaatatt ttcttgtgta aggtttggaa atgtcatggg gtcaagtgga tctgttattc
   133261 ctctttttag taaacaaata aaaaacaata aagatattac tttgacagat gttgatatga
   133321 cacgatttat gatgtcaata ccggatgctg caagattagt tctgtctgct gctatgaatt
   133381 caaaatttgg agaaacatat gtcttaaaaa tgccggcaat caatatttta actttagctc
   133441 aatcattaat aaaaatatat aatgatacac atgatgaaaa atatagaggt gaaataaaag
   133501 ttgtaggtat caggccagga gaaaaattat atgaagagct aatgactaca gacgaaattg
   133561 aacgctcata tgaaaatgat aaaatgtata tcattccacc acactatcat gctactgacg
   133621 aactgtacaa agattatgaa aaaattaaaa tcactgcata ctcttcagat gcaacagaat
   133681 taatgagtgg acaggaattt attagaatcg caaaagagaa taatgtcgtg acttggtaag
   133741 gagttttatg atgaaatcag aagtatatgt aattgcagaa attggtgtaa accataatgg
   133801 aagtctagat ttagctttag aaagtattga taaagctaaa gaatgtggag ctaacgcggt
   133861 taaatttcaa acatttaaaa cgaataagct agtttccaaa aaggctccaa tggcaaaata
   133921 tcagaaggat aatcttaaag aggacagttc tcaatttgaa atgctaaaaa aattagaatt
   133981 atctcaatct gattttatta gaataaaaga ttattgtata caaaataaaa ttgatttttt
   134041 gtctactcct tttgatgaag aaagtgcaga ctttctaaaa caaattggtg tacaaggatt
   134101 taaaattggc tcaggagatt taacaaattt accttttctt agaaaaatag atatgtatgg
   134161 gttgccagtt ttattatcta caggtatggc tgatattcat gaggtcagtg aggctgtaga
   134221 ttgtttcata gatagtccag ttactatttt acattgtact tcaaattatc cggctagttc
   134281 tgaagatatt aatttattgg cgattaacac attaaaagaa atgttcggga tacccgttgg
   134341 gtactccgat cattcattgg gatatgatgt tggtatttgt gctgttgcga tgggtgctaa
   134401 ggtaattgag aaacatttta ccttggataa ggaactacct ggtccagatc ataaagcttc
   134461 tttagatcct caggaatttg cagattttgt aaagcatatt agaaacactg aaatattttt
   134521 aggtgatggt gttaagcgag caatgccttc tgaacttagt acaaaagaag tatcaagaaa
   134581 aagcctagtg gtcactgaga atttaaatgt gggagatatt cttacagaga aatctcttac
   134641 aataaaacgt cctggaaatg gaatagctcc taagttttta tatcaatata ttggcaaaaa
   134701 agttaaaaga ccaataagtg cagatagtgt tctgcttgag gaagatgttg aatgaaaaaa
   134761 atacttatag taagtggaac acgagctgat tatggaatat ataaacctgt tgctgagaat
   134821 ttactacaag ataattggga tgtatcattt gttgtttctg gaatgcattt atcccataaa
   134881 tatggattta ctaaagaatt gatcttagct gacggattta gaattagtgg agaagttcaa
   134941 agtttgtttt tagagaacaa taagggaaac atggctcggt ctgtggcatt agagataaat
   135001 ggtttcacaa atatatttga acaaactagc cctgattttg tattattact aggggataga
   135061 ggagaaatgt tggcagctgc gatagcttgt ttgtatcttg gaatcaaaac agcacatatt
   135121 cacggaggcg aagtgtctgg tactgtagat gaatcaataa gacatgccat aacgaaaata
   135181 gcatctgttc attttgctgc tacggaaaaa agcaaagagc gtttaataaa aatgggggaa
   135241 gattcttgga gagtttttcc cgtcggagct cctagaatag atactatttt aaataataga
   135301 ttacctagtt ttagagatat aaaagaaaaa tatcatctaa aatttgaaaa gaaaaaatac
   135361 gttttgcaag tatttcatcc agttgtaacc gaactagaaa atatagaaaa acaaatttta
   135421 gctttgatca attcaatgga acaacaatct caaccaatta tttgcatttt acctaatagt
   135481 gatgctggta gcgaaattat cagaaaaatg taccagaaat atttaaaaac ggatattatt
   135541 tatattacga atttagagcc ggaggaatac ctgactatat taaaagagtg caaatttatg
   135601 atcggaaatt cttcttctgg gataattgaa gctgcatcat tcaaaatacc agtaataaat
   135661 ttaggaacca gacaaaatgg acgagaacaa tctaaaaata ttataaatgc aaatctaaat
   135721 gaaaatgaaa ttgaaactgc tttgaataaa atacaagatc ctcagtttat aaaaagcttg
   135781 aaaaatgtta agaacatcta cggtgacggc aacagttcta gaagaataac aaaaacattg
   135841 aatcaaatat tgaatgatgg ggaagatatt aagtggattc aaaaacaaat agcttattaa
   135901 aatatactag tgatccatac attattattg gggctggcgg acacgcaaaa gtcattattg
   135961 aaaccttaag attagaaaat aagttaatat atggattttt tgatgataag caaaataaaa
   136021 tgcaagatct gaaatatctt gggaaaataa acgaattgaa tagctgggcg gatagcaaaa
   136081 aaaaaatcta taaatttatt gtagctattg gtgactgtgt agtaagaaaa aagattataa
   136141 aaaaattgaa tatgccaagt gagcaatatg gaatagctgt tcatccaagt gctatagtgg
   136201 caaaagaaat ttcaattggt tatggaactg ttatttttgc gaatgctgta attaacccag
   136261 atgctgtaat tggagagcat gctataataa acacaggtag cattgttgag catgattgta
   136321 gaattaataa ctatgttcat ttatctccag gtgtatgctt agcgggtgga gtacatgtag
   136381 gtgtaggaac tcaaatagga attggttctc aatgtatcca gatgaagaaa attggatctt
   136441 ggtgtatgat aggcgcaggg agcactattg ttaaggatat tccctcacat tccttagctt
   136501 atggcaatcc agctaaaatt aagaaagaag gaattaattt tgaattctag aattttatta
   136561 tcaagtcctc atatgggagg aacagaagaa aaatatgttg ctaaggcttt taaagataat
   136621 tggatagcgc cattaggtga aaatgtggat aaatttgaag aaagtattac aaaatatact
   136681 ggttctaagg gcggagcttc tgcgttaagt tcaggtacag ctgccataca tcttgcctta
   136741 agactgctta atgttggaaa aaacgatatt gtctttgctc cttcatttac ttttatagct
   136801 acagtaaatc ctattttata tcaaggggct gtaccagtgt tcattgactc agaacctaat
   136861 acatggaata tgagccctgt tgcactagag agcgcattag aagaagctga aagaaacaac
   136921 caattaccta aggcaatcat cgtagtgaat ttatatggtc aaatggctga ttatgaacga
   136981 attttagcta ttgcaaacca atttaatgtt ccagtgatag aagatgcagc ggaatcgtta
   137041 ggttctactt ataagggaat ttcgtcaggc ttatttggga caattgggat ctattcattt
   137101 aacggtaata agatcatcac aacctcaggt ggaggagctc tagtgtctag tgataaaaaa
   137161 tatgttgagc attcaagatt tttagcaaca caagcaagag atcaagcacc gtattatcaa
   137221 catagcgaaa taggttataa ttaccgtcta agcaatattt ctgccggtat cggacgtgga
   137281 caaatggaag tattgaatga tagagtagct aaacgaagag aaattttcaa tcgatataag
   137341 gaagcttttt ctggaattga tagaattgaa atgatgccag aagtggatgg catggtgcca
   137401 aatcgttggt tgtctacttt aacgatcaat gattataatt ctattgatca agtagttaac
   137461 gtaataaata aactaaatga aaaaaacatt gaaacaagag ctttgtggaa accactacat
   137521 ttgcaaccag tatttcaagg ggtaaaaatg tattaccatg ataataatct tagtgtgtca
   137581 gaaaagttat ttaatagcgg cttatgctta ccttctggaa gtaatatgac agatagtgaa
   137641 caagaatttg ttattgatga gtttaaaaaa atattcaagt aggtgtgata ttttatgaac
   137701 ataggaatta ttggtttagg ttccatgggg aaaagaagac ttagactcat tcaagaacac
   137761 tttaatgata ttttactatg tggtgttgat actaatgagg cgagacgaaa ggaagttgaa
   137821 aaggaatttg gaataccaac ttataatagt ctaacagagg caaaaaattt gtttgaaata
   137881 aattcagttt ttgtttgtac atcccctata acccatgaat ttattataaa agaggcttta
   137941 aatttagatt gtaatatctt tacggaaatc aatttattaa atgaatatta tgatgaaata
   138001 atcgaagtag ctaaaaagaa agatagatta ctttatctat cttctacatt tttgaagaga
   138061 aaagaaattc aatatattaa gtctaaagtg acgcataata aaaaaatttc ttatcaatac
   138121 catgtaggac agtatttacc cgactggcat ccttgggaag attatcgcaa tttttttgtt
   138181 tcaaacaaaa aaactaatgg gtgtagagaa atattcgcta ttgaattacc ttggattata
   138241 gatacatttg ggaaagttaa atcgtttact gtaatgaccc agaaattatc caatcttgat
   138301 attgattatt cggacagttt cactcttatt attgagcatg aaactggtat agttggaact
   138361 ttaagtgtag atgttgtttc aagagtagct aaaagaaatt taactataat tggtgaaaag
   138421 attttaatag aatgggaagg taacccggat tctctatttt catggaatga agaaacttct
   138481 agtatgacag atattcaatt gtacgatgag gttatgcatg ataataatta ttcacatact
   138541 attattgaag atgcatattt agaggagatt aaagaatttt tcacgctttt ggaaaagaaa
   138601 atggaacccc aatatacttt tgaaaaagat aagtatatta ttaacttaat taacgacatt
   138661 gaggtaaatt aatgatgaaa gtaatttttt ttgggttagg gtcaattgga aagagacatc
   138721 taaaaaatct aataagctat ggaaaaaaga attctattaa ttttgatata acagcttata
   138781 gtagcagtgg aaaaaaagag tttgaagaaa taacgtatat tcaaaataga gacgatttgg
   138841 atacacatta tgatatagct tttattacaa atccgacttt taagcatctg gaaacactaa
   138901 atgtaattgt tgaaaaagtt gattacatat ttttagaaaa acctgctttt tctgagagta
   138961 ttgatcttac tcaatataat tatgattaca aaaatattta tgttgctgct ccactgaggt
   139021 ataaaaatat catggaagtt gttcgtaata gtattcaaag taaaaaagta tattctgtaa
   139081 gagctatttg ttctacttat cttcctgatt ggagaaaagg tactgattat agaaataatt
   139141 attctgcctt taaatctatg gggggaggag tagaattaga ctgtatacat gaattggatt
   139201 atattataaa cctatttggt ttccctaaat cagtaaaaaa acagtttggt aaatattcga
   139261 atcttgaaat taacagtaat gatttagcaa catatatatt gagatacgat gataaaatta
   139321 tagaaattca tttagattat tttggaagag ttcctacaag aaaatttcaa ttgtttactt
   139381 tagatgaatg tatcaatgtt gacttattaa ataataaaat aagttttgaa gaaaacattg
   139441 ttaaagaaat gcctgaatct gctaatgata tgtatgagaa ggagttgaaa tatttctttg
   139501 aaaacgtagt aacaggcaaa gaaaactgga atgatttata tcatgcaaac gaagtattga
   139561 aaatagctga ggagaaataa atggaaattt tatttactat ttgtggtaga gctgggtcta
   139621 aggggtttaa aaataaaaat ttgaaatatt tggcaggaaa gcccctagtc catttcgctt
   139681 tagcaatgat agatatctat caacaaaaac atcctgaaca taattgcacg acttgtttga
   139741 atacggacag taatgagtta gtagaattga cgactaagtt aaatccgtct gtgaaaattg
   139801 tagaacgtaa acctgaatta gctacagata ctgtagggaa aattgaagtg atacgtgatt
   139861 cattcttaca actagaaaga gaagataata tatttgattt aattatagat ttagatttga
   139921 cttcaccttt acgtgaagtt tctaatcttg aagaattaat agatgaacat ctaaaaaatt
   139981 tagataaaga cgtctacttt tcagttgttt ctgcaagacg taatccctat tttaatatgg
   140041 taaaaagaat tgaaaatgaa gttactatag ttaataaatc aagatacaca tctaggcaac
   140101 agacacctga agtttttgaa atgaatgcct caatgtattc ttttaaacct gaatttctaa
   140161 aaaaaagtaa ttatattttt gatgggcaat gtgggataat aaaaatgaga gattatctaa
   140221 ttttagatat tgatagtgaa gaagatttta aatggttaga atttttgttt cctaaatttt
   140281 tagaagattc ttcggagctt caagatatct ataattattt atatcggagg taaaaggatg
   140341 agctgttatc ttatcaccgg tggggctggc ttcattggtt ctaccttagc caatcattta
   140401 ggagaagaac atgtggtaat cgtcgtcgat gacttatcaa tgggaaaagt agaaaatctt
   140461 gatatgacga aaaatatcac ttttattgag ggagatgtcg ctgagaaatc cttgatggaa
   140521 gagatcatga aagctaataa atttgattat atctttcatt tagcagcagt tgctagtgtg
   140581 gcagattcag ttgcccgacc agtagaaaca catcgtgtga attttgaaag tgtattaatg
   140641 attttagaat tgatccgcaa gtatcaacct gatttaaaac ggatcgtgtt tagttcttca
   140701 gcggctgttt atggggatga accgacttta ccaaaaaaag aagagtccgt gattcgccca
   140761 ttgacgcctt atgcggttga taaattcgct gcagaacaat atgtgctaga ttattgtcat
   140821 ttatatgatg tacccggtag cgcagttcgt tttttcaatg tctatggtcc aaatcaaaat
   140881 ccgaattctc catacagtgg tgtgatttct attctcgtag atcgctataa gaaacaaatc
   140941 gtaggagaag caacttcatt tacattattt ggtgatggaa gccagtctag agactttgtt
   141001 tatatagatg atgtaatcca agcattactt ttagttgcga atgaggaagc tgcactgggg
   141061 aaacaattca atgttggaac gggtaaagca accacacttt tggaattgat tcatgcaatc
   141121 aatgaaattc tcggtgtaga attgacactt gaatacaaag aagagcgttc gggtgatatc
   141181 catgactctc tagcagatat tactaagatc cagtcaattg gctatcaacc gaaatatggt
   141241 attttaagtg gaatgactaa ttatttgaaa actgaaatca attaaaacat actaagataa
   141301 gattagaggg taaattcagg ctctttgtca ataaggactg atgagttttg taaaatcaaa
   141361 tctgggatag aa
//
LOCUS       DACDXO010000003        91843 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E23605_2017
            SAMN12639989-rid9881463.denovo.003, whole genome shotgun sequence.
ACCESSION   DACDXO010000003 DACDXO010000000
VERSION     DACDXO010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639989
            Sequence Read Archive: SRR10023389
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 91843)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 91843)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741914.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:42:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,972
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 33 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 17 of 73
            Pseudo Genes (multiple problems)  :: 10 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..91843
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639989-rid9881463.denovo.003"
                     /isolate="E23605_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            375..1082
                     /locus_tag="IWM35_000272"
     CDS             375..1082
                     /locus_tag="IWM35_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP9918157.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRGDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(1142..1786)
                     /locus_tag="IWM35_000273"
     CDS             complement(1142..1786)
                     /locus_tag="IWM35_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HAP9918158.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDQAKSFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGVPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSADHFKESIIELLNGQVQFEEGPVSYHEQLVTDHI"
     gene            1913..2200
                     /locus_tag="IWM35_000274"
     CDS             1913..2200
                     /locus_tag="IWM35_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9918159.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            2308..2628
                     /locus_tag="IWM35_000275"
     CDS             2308..2628
                     /locus_tag="IWM35_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP9918160.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIT
                     LIGE"
     gene            2709..4022
                     /locus_tag="IWM35_000276"
     CDS             2709..4022
                     /locus_tag="IWM35_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAP9918161.1"
                     /translation="MNHFWGRRLLTRDIKAIDCEEVNLPNYEKIAAVERLGNWILCHR
                     CGMRTSVIDGQLADYGYFCTHCLYLGRCDTQQDLYLFDQPEAKPREVVFFWTGQLTEK
                     QTEIAKRILCHSEKRHHLIWAVTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNEL
                     FLRYRYVFPEEKITLLHGNTATAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYN
                     GDAGLAHAVQTAIKPDGRFIYLSATPDKKLLKEIKQTFDIHKLALRFHRRLLPEPVLV
                     FMNNWRQKCKNKKKIKSLVRIIHQLIKENHILLFCPSITMMHRLKATLQEALPRYEIT
                     DVSSRDNQRAEKVQKMREAKYDILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQ
                     IAGRADRKGPYTNSQVYFFYEEKTAAIRKACQEIKEMNEEGKKML"
     gene            4019..4738
                     /locus_tag="IWM35_000277"
     CDS             4019..4738
                     /locus_tag="IWM35_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HAP9918162.1"
                     /translation="MRCSCCSKDIVQNLTFSEIIGVSKYRLPQTCSVCRAQFHLLETR
                     GCSGCQKPIHFNLESTKKKVKSIPVYCSDCQEWKKKYPTYDFQHRAFFVYDENMKAWL
                     NQYKFMGDIRLAGTFAYKWTQIKKEYPTYIYCPIPLSEKRRTERGFNQVSEMLKAARI
                     PFQMLLKREKHLLAQAKNTRKERLEMPQPFELAVTFSKIQNKKILIIDDVYTTGQTLF
                     HAADCLQVAQPKIIRTISLAR"
     gene            4866..5417
                     /gene="raiA"
                     /locus_tag="IWM35_000278"
     CDS             4866..5417
                     /gene="raiA"
                     /locus_tag="IWM35_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HAP9918163.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTADATALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
     gene            5639..8173
                     /gene="secA"
                     /locus_tag="IWM35_000279"
     CDS             5639..8173
                     /gene="secA"
                     /locus_tag="IWM35_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HAP9918164.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPHHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKSFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTAQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
     gene            8299..9400
                     /gene="prfB"
                     /locus_tag="IWM35_000280"
     CDS             join(8299..8361,8363..9400)
                     /gene="prfB"
                     /locus_tag="IWM35_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_078989099.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HAP9918165.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRGLFDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            9505..10191
                     /gene="ftsE"
                     /locus_tag="IWM35_000281"
     CDS             9505..10191
                     /gene="ftsE"
                     /locus_tag="IWM35_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HAP9918166.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            10175..11068
                     /locus_tag="IWM35_000282"
     CDS             10175..11068
                     /locus_tag="IWM35_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9918167.1"
                     /translation="MDTMIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFM
                     AVIMNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELK
                     KIQNEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSD
                     RIFQIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRW
                     PFFLEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIHPQNIVWKINLLMV
                     GIGVIIGSLGSIISMRRFLKF"
     gene            11220..12668
                     /gene="cls"
                     /locus_tag="IWM35_000283"
     CDS             11220..12668
                     /gene="cls"
                     /locus_tag="IWM35_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HAP9918168.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWVLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEVAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGKIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYTLQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHSTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLVYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
     gene            13005..13862
                     /locus_tag="IWM35_000284"
     CDS             13005..13862
                     /locus_tag="IWM35_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HAP9918169.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQTENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            13977..14897
                     /gene="pstC"
                     /locus_tag="IWM35_000285"
     CDS             13977..14897
                     /gene="pstC"
                     /locus_tag="IWM35_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HAP9918170.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTVTTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            14897..15781
                     /gene="pstA"
                     /locus_tag="IWM35_000286"
     CDS             14897..15781
                     /gene="pstA"
                     /locus_tag="IWM35_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HAP9918171.1"
                     /translation="MNAKRSDKIATGVLYIVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVIKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            15796..16602
                     /gene="pstB"
                     /locus_tag="IWM35_000287"
     CDS             15796..16602
                     /gene="pstB"
                     /locus_tag="IWM35_000287"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HAP9918172.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDLHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            16618..17376
                     /locus_tag="IWM35_000288"
     CDS             16618..17376
                     /locus_tag="IWM35_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HAP9918173.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGIDLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            17389..18066
                     /gene="phoU"
                     /locus_tag="IWM35_000289"
     CDS             17389..18066
                     /gene="phoU"
                     /locus_tag="IWM35_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HAP9918174.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            18304..19335
                     /locus_tag="IWM35_000290"
     CDS             18304..19335
                     /locus_tag="IWM35_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HAP9918175.1"
                     /translation="MQKKISIITIFFICVLTSWVIQHQAALYEVPVIEIEKVRIKEDH
                     QLITGKLMNTDQKGESIYLSVPYQKNQIETANIKARQMYLVQMHGDEWQLVAKKNDGW
                     LFFFTSIFIGAMLLIGGKQGLFALGSLIINIVLLLLLLLIFTKTEGISLLNISILFVV
                     IGMIISILALDGWNRSSIIKISAAVTSVFLAFFICLLTMNHWKDQGLRYEELNYLTRP
                     YRSVFLSSVLIGTAGGTLDTVITVIATLEELTKRQPEITAKQLWLSGRQVGRDVIGSM
                     TNILLFVYISGSVPSLLLYLGNQWSFKETIEQHLSLEFLRVIAGSLGIVLSIPISVLF
                     FLTVRRLKR"
     gene            19332..20099
                     /locus_tag="IWM35_000291"
     CDS             19332..20099
                     /locus_tag="IWM35_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HAP9918176.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTSVELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALLVLPLTCVTTAYLFTKKKEQLKME"
     gene            20199..21767
                     /gene="liaX"
                     /locus_tag="IWM35_000292"
     CDS             20199..21767
                     /gene="liaX"
                     /locus_tag="IWM35_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HAP9918177.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDQASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQTLEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDGEIAQVQL
                     STTTGSIYLKDTDK"
     gene            22067..22384
                     /locus_tag="IWM35_000293"
     CDS             22067..22384
                     /locus_tag="IWM35_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HAP9918178.1"
                     /translation="MKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVAF
                     GSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTREQTKKRKEAEKIDDDEW
                     SDF"
     gene            22384..22734
                     /locus_tag="IWM35_000294"
     CDS             22384..22734
                     /locus_tag="IWM35_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HAP9918179.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALLVAILM
                     SLVNVIVSEHNSRK"
     gene            22901..23839
                     /locus_tag="IWM35_000295"
     CDS             22901..23839
                     /locus_tag="IWM35_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HAP9918180.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANENGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            23854..24684
                     /locus_tag="IWM35_000296"
     CDS             23854..24684
                     /locus_tag="IWM35_000296"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HAP9918181.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLFLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            24728..25753
                     /locus_tag="IWM35_000297"
     CDS             24728..25753
                     /locus_tag="IWM35_000297"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HAP9918182.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHRASQVEEINTQ
                     HTNQRYLPDHYLPDSIQAFTNMEDAVKDADAVLFVIPTKAIRSVAKELVPKLNTKPVI
                     IHASKGLEQGTHKRISDILLEEIPAEKRKAVVVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNNYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGIGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQMMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            25768..26706
                     /gene="galU"
                     /locus_tag="IWM35_000298"
     CDS             25768..26706
                     /gene="galU"
                     /locus_tag="IWM35_000298"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HAP9918183.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYILSLGKTLEAEGSKIDHNPKGITEIKEEKKK"
     gene            26831..27271
                     /locus_tag="IWM35_000299"
     CDS             26831..27271
                     /locus_tag="IWM35_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HAP9918184.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTKDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIATKVGGIGKTTAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            27274..27876
                     /locus_tag="IWM35_000300"
     CDS             27274..27876
                     /locus_tag="IWM35_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAP9918185.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSTELTGIAKEKAQDLSQQAGDLAGSVKQKVSESADEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDMKKPADELGSIAKDTSEDIMIDVKDAAGKAGDTVNEG
                     MSEAKPVADDSKEMMKMAKEDAKEAKKEIKDTMTDSSTNH"
     gene            complement(27935..29041)
                     /locus_tag="IWM35_000301"
     CDS             complement(27935..29041)
                     /locus_tag="IWM35_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HAP9918186.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVERFDAISDILTGTDSYTDLTSIIQRLQLIKTLEEKDKLIEAGKWADVAF
                     EIGFKAVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGNTKISP
                     NELVLFDLGVVWNGYCSDATRTIAYQKPTDFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLEK"
     gene            29251..30252
                     /gene="ccpA"
                     /locus_tag="IWM35_000302"
     CDS             29251..30252
                     /gene="ccpA"
                     /locus_tag="IWM35_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HAP9918187.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            complement(30589..32934)
                     /locus_tag="IWM35_000303"
     CDS             complement(30589..32934)
                     /locus_tag="IWM35_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HAP9918188.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLMFAA
                     LGIGIGAGYFAYLVEDTQLPTKKALQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIILSAQVEKYFSKDEIIATYLNVSPFGRNNKGQNIAGVQEAAK
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSDAYYQKAQQTIQNKGYTIHSTIDKDVYAALQNGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLIGSES
                     RVSDYATTWQEGENAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINAKITTPFKDAISSLNGNLGKA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSKARIRSANYLANLINQAY
                     QAKPDIFGTDEKFELSSDVMKKKVSGFTGQTPGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANQATKESDEKDD"
     gene            33213..34115
                     /locus_tag="IWM35_000304"
     CDS             33213..34115
                     /locus_tag="IWM35_000304"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HAP9918189.1"
                     /translation="MLLGSHVSMSGKKMLLGAAEEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAYMAEKGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGTEAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKIIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     IAMFKAQTFDPEVFPELLAEEVIL"
     gene            34328..36103
                     /locus_tag="IWM35_000305"
     CDS             34328..36103
                     /locus_tag="IWM35_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HAP9918190.1"
                     /translation="MLNTLKPLINQLKKKHTYFILGIALLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIRYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     IFASILVALAFKPIVKNKYLLLSIYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYGSSRKLLPWFLGLSVAFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEVKKLKKRLFLLSKREWLKLSLFLLPIVGFSIVYQWIEYKNETTYGIATIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALAASPTFHTVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYHKGT
                     IKKKKEFYLMGSGDGKTMSDLPIVFDFLQSGYAYAWRYKDFHQGGNYSFGTQEQVKQA
                     QELLNLPLMENWIDSNEPRTPNFQLTKSAKVANVIIKIYQVLAIPVLIASIAGILLMI
                     LGTVFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
     gene            complement(36169..37755)
                     /locus_tag="IWM35_000306"
     CDS             complement(36169..37755)
                     /locus_tag="IWM35_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HAP9918191.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVSQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQNEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHAALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFIVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADHIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQLLQETVEEIE"
     gene            38006..38230
                     /locus_tag="IWM35_000307"
     CDS             38006..38230
                     /locus_tag="IWM35_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HAP9918192.1"
                     /translation="MLSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            38372..40123
                     /locus_tag="IWM35_000308"
     CDS             38372..40123
                     /locus_tag="IWM35_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP9918193.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGVFSLFAVALVQLVPPKVIGIIID
                     EIADKNLSIKIIFGWIFLLIIAAFLQYLFRYIWRTNIWGSAARLEKELRQQLFKHFTE
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIAMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPAKG
                     ELNVAISSFNYPKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGRSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     QMWEKQQLEAKIEGGEA"
     gene            40123..41907
                     /locus_tag="IWM35_000309"
     CDS             40123..41907
                     /locus_tag="IWM35_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9918194.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRRTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGMYLFGVIVKSIIWFFQWYLYSM
                     ASLKTYQYIRVKLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFLAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     VLTLFGIDALHSPVEVGLIYAFTTYVQGFFNPMSQMMDFLSIFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQMITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPAITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLAKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGI"
     gene            42233..43084
                     /locus_tag="IWM35_000310"
     CDS             42233..43084
                     /locus_tag="IWM35_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP9918195.1"
                     /translation="MLIEKVLNNNVVITRDERNQEMIVMGKGLAFKKKVGDPIFDSCI
                     DKKFQLTNDGLSQQFQELAQAIPLEYLEISCEIIEYTKEALQVQLNDSVYIALTDHLY
                     TAIERHKMGIFVPNVLLYDIKQLFPKEYAIGKKTVKKISDKYHIKLSEDEAGFIALHL
                     VNAQGIQSQQDNTYLFTITIQDILNIVRYYYKITFDEDSVHFFRFVTHLRFFLTRVLD
                     GEKSEVGEVSEELFQVIQKKYKDTFGCVEKIADFLAETFNYQMSTDEKMYLTIHTARL
                     VHENRCK"
     gene            43138..44889
                     /locus_tag="IWM35_000311"
     CDS             43138..44889
                     /locus_tag="IWM35_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9918196.1"
                     /translation="MAKPSTNKLEGIEMGKYQELARKIVENVGGKENINGLTHCITRL
                     RFKLKNEEKANDEILKNMDGIVTVMRAGGQYQVVIGNHVPVVFEEVIKAGNLTFDEAV
                     STEKMRPFDMLIDGISGCFQPFLAILAAGGMIRGLTAFLVFLGAFDRGSGTFVMFDNI
                     GDSVFQFMPVIIGLTAARKFKVNEFVGMLLGAALMNPSLSLEALSGAAEAPLTTIFSG
                     TIFEAPIYQTVFGIPWIARNYASSVIPIIFIVLLASQIQKPIKKLVPEMIANFFVPFF
                     TVLITMPLGFLLVGPVFTFATDILMAGFETLLALSPVIYGAIVGFFWQILVMFGLHWA
                     IVPMGLMQFSVNGWQNIMTPVAVVSFGQTAALTALYFKLRNPKDKAIAIPAIVSGIVG
                     ITEPAIYGFTLPRKKIFIFTCVGGALGGAYSGLMNLTSWNQGGLGIFTIPNYIRPDGD
                     LTDVINVLIGIAIAMVVSFTLTFFFWKDEAGETDDIQKKSGKEIVKTPIQGQIAPLNA
                     AKDAAFAQGTLGRGILIYPEKGEVRAPFDGTVMTLFPTKQAIGMVSETGLELLIHVGL
                     DTVQLEGKYFESLVQQG"
     gene            44893..45063
                     /locus_tag="IWM35_000312"
     CDS             44893..45063
                     /locus_tag="IWM35_000312"
                     /inference="COORDINATES: protein motif:HMM:NF012578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9918197.1"
                     /translation="MKQGDLLLRFDKKKIEKEGYSLETPVIVTNYMDYLDILENQGET
                     VGPDDALITALT"
     gene            complement(45104..46006)
                     /locus_tag="IWM35_000313"
     CDS             complement(45104..46006)
                     /locus_tag="IWM35_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP9918198.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQEIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIQDTLSLIHHDQLQKAKQLMLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNHVPILALTSIGDST
                     LSSMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVD"
     gene            46236..47639
                     /locus_tag="IWM35_000314"
     CDS             46236..47639
                     /locus_tag="IWM35_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAP9918199.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMATMTEPAMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     DLIDRLYDEDNGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLPADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMDELFIAHQQYLPQFKETISRLKAEGIEVQ
                     DELARELSSNTHVMEEV"
     gene            47639..49072
                     /gene="ascB"
                     /locus_tag="IWM35_000315"
     CDS             47639..49072
                     /gene="ascB"
                     /locus_tag="IWM35_000315"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAP9918200.1"
                     /translation="MGFRKDFLWGGATAANQCEGGYDEGGRGLANVDVVPIGEDRFPV
                     ITGKKKMFEFDKDHFYPAKIAIDMYHHYKEDIALFAEMGFKTYRMSIAWSRIFPMGDE
                     NEPNESGLKFYEDVFKECQKYGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENLC
                     RVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEEGENEEQIKYQAAHHELVASAI
                     ATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPAY
                     ALKEMERNGIQIKMEPGDAALLKEHTVDFISFSYYSSRVSTTDNELLEQTAGNIFASV
                     KNPYLEASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDYR
                     IDYLAAHIQAMKDTVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNEGNG
                     TLARSKKKSFNWYKKVIDSNGENLSND"
     gene            49217..50143
                     /gene="trxB"
                     /locus_tag="IWM35_000316"
     CDS             49217..50143
                     /gene="trxB"
                     /locus_tag="IWM35_000316"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HAP9918201.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYEGVSRFGTENAYGIVQDIKDHGSHKEVICEDKTYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNIPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            50357..52084
                     /locus_tag="IWM35_000317"
     CDS             50357..52084
                     /locus_tag="IWM35_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HAP9918202.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLIDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEETKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDDEAEHSGLITIIGEEVDAAYLEEIKAV
                     TIDHELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAMRLGEKEGADLLIATDPDADRLGAAVRLPNGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYYAEKTISVTMSGQEGSAKISALMEKFREEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGNSSEDAAEKVAAYE
                     AIIKKMTEE"
     gene            52157..52450
                     /locus_tag="IWM35_000318"
     CDS             52157..52450
                     /locus_tag="IWM35_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HAP9918203.1"
                     /translation="MPDTQKTKVEKVSQLFKMLSDPTRLKILLYLKDGEQNVTAITQA
                     VEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHVLDILNQTLKHVEHQ"
     gene            complement(52544..53404)
                     /locus_tag="IWM35_000319"
     CDS             complement(52544..53404)
                     /locus_tag="IWM35_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HAP9918204.1"
                     /translation="MSSDSTEIVTFFVISDSAGETATKLAQATMAQYPTVEFNLFRRT
                     FVTDEKTLMQALQDALKEKALVLHTLINQELIEMTRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEETD"
     gene            53496..53924
                     /locus_tag="IWM35_000320"
     CDS             53496..53924
                     /locus_tag="IWM35_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAP9918205.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGEIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            54086..54262
                     /locus_tag="IWM35_000321"
     CDS             54086..54262
                     /locus_tag="IWM35_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HAP9918206.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            54289..54735
                     /locus_tag="IWM35_000322"
     CDS             54289..54735
                     /locus_tag="IWM35_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HAP9918207.1"
                     /translation="MSLLSRLNDDMKTAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
     gene            complement(54891..56270)
                     /gene="brnQ"
                     /locus_tag="IWM35_000323"
     CDS             complement(54891..56270)
                     /gene="brnQ"
                     /locus_tag="IWM35_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HAP9918208.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGANWV
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYNSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFHPMGNLNQSAADTYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMSLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKTPFVYQVTMLFAAVPAVLDM
                     LANSPALVSQQRVVASMLEFYHHHVPFAALGLGWMVPTLLGYAGSLLFYYAYRLSGYK
                     QEANELPEE"
     gene            56976..57947
                     /locus_tag="IWM35_000324"
     CDS             56976..57947
                     /locus_tag="IWM35_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HAP9918209.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            57971..60163
                     /locus_tag="IWM35_000325"
     CDS             57971..60163
                     /locus_tag="IWM35_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HAP9918210.1"
                     /translation="MLNKTLQKLQKKLGKAALPFLLFLFFIIVCGVTFSSVKQKANDF
                     REGQVAEESIRANKTIENTEETEQRRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDTINKSYNDKVDKAKEDENIPKPTTEERIATLKKNFENVNAENVAFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEDQIQYLNVTTEQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNAGERTKYVLFYVTAMSVSVV
                     LMKFFQLFQTEQAAYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTVILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIIYQGMSFGD
                     ATTWLTLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICRQHHGTTLMRYFYVKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKSEVEKIKEEQEE"
     gene            60180..60656
                     /gene="ybeY"
                     /locus_tag="IWM35_000326"
     CDS             60180..60656
                     /gene="ybeY"
                     /locus_tag="IWM35_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HAP9918211.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLELPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISTER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            60634..61035
                     /locus_tag="IWM35_000327"
     CDS             60634..61035
                     /locus_tag="IWM35_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HAP9918212.1"
                     /translation="MPMDLKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFG
                     ILALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKD
                     MAAGAVLVTACFSVIVGLILFVPKIYHLLFN"
     gene            61050..61949
                     /locus_tag="IWM35_000328"
     CDS             61050..61949
                     /locus_tag="IWM35_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HAP9918213.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            62092..62904
                     /gene="recO"
                     /locus_tag="IWM35_000329"
     CDS             62092..62904
                     /gene="recO"
                     /locus_tag="IWM35_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HAP9918214.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKGIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRHIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQLMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKYGGVICERHWSMDDHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRELIDQLYDEYVGIHLKSKKFIDQMKSWAHVLKTSEKDMSEKDLPKKEE"
     gene            63290..64207
                     /gene="glyQ"
                     /locus_tag="IWM35_000330"
     CDS             63290..64207
                     /gene="glyQ"
                     /locus_tag="IWM35_000330"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HAP9918215.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPENVKEAE"
     gene            64209..66284
                     /locus_tag="IWM35_000331"
     CDS             64209..66284
                     /locus_tag="IWM35_000331"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HAP9918216.1"
                     /translation="MAKDLLLEIGLEEMPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKESKEVLTGLADVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEVIPFEILDVKTGRASRGHRFLGEDVTFAHAKEYEEKLNEQFVIASPQKR
                     QEMIVTQANELAEKNHWVIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLENVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGELPQSTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISD
                     VISKDTDRFGIGLSEGQQQVIDFIKGRLRQLLTTKNIRHDVIDAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAQLEKQSEVDPELFENDAEKELHKAVEA
                     MKASFADQTIHENYDQLVALRPLIENYFDQTMVMADEPAVRKNRLTQLSQIAAMALSL
                     ASLDQIITK"
     gene            66669..67448
                     /locus_tag="IWM35_000332"
     CDS             66669..67448
                     /locus_tag="IWM35_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9918217.1"
                     /translation="MEKPFVKLLATVAVGTGAIVICLFGYHFNNQRQHHQRINYAESA
                     ITNQKDTVTSLSKEVDKLYSTKEKIFLNPEITEETVSNLSHKLSSIKLSADDYDIKES
                     ELPKEAAAIQEEKKAVLTQLEDAESKLKIQTAVNDLFTKNVSNWQQAVDDVVIKEKVT
                     SADVARVRENMSFFKDSPWKTVVMQYLGFADTQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTALSLIEQIRNEKIRAAYASKAETVAQQMGYSNTSY"
     gene            67489..68433
                     /locus_tag="IWM35_000333"
     CDS             67489..68433
                     /locus_tag="IWM35_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP9918218.1"
                     /translation="MAKRRRRSRGKKIFLIILAILVVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGHDTKDKINHAYAFGGAAMAMDTVQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFTIGKITLNGDQALSYSRMRYEDPNGDYGRQER
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMSFDEMKKIALDYRDAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            68449..69228
                     /locus_tag="IWM35_000334"
     CDS             68449..69228
                     /locus_tag="IWM35_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein kinase"
                     /protein_id="HAP9918219.1"
                     /translation="MEETISLKEIFETLRKHVITILISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     GQIKDAISVNQSQNSQMFSIQATSTNAVTAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLVIGLVLGMMVGVGLAFLFELLDRTVKDEKYVTDTLGFPILG
                     TVPEMSAKELNAGIRKKGPAPTQPKATNDKTAPSRRSRSRV"
     gene            69241..69939
                     /locus_tag="IWM35_000335"
     CDS             69241..69939
                     /locus_tag="IWM35_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HAP9918220.1"
                     /translation="MARTQKQKAAQTHAVSLVTLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVIYKTFKLNNAS
                     GLSTVLSSSGSVADEIQRTPVENLSILPSGPKPPNPSELLSSPRMDQILAEARQLFDV
                     VIFDMPPVVAVTDAQIMSSKTDGTLLVVRENMSRKESLTKAKELLDMVQARVLGVVYN
                     GAEHSKDAGYYYGN"
     gene            69967..70731
                     /locus_tag="IWM35_000336"
     CDS             69967..70731
                     /locus_tag="IWM35_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein phosphatase"
                     /protein_id="HAP9918221.1"
                     /translation="MIDLHCHILPGIDDGAENIDASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIDRDEILFTDLD
                     DTYLLIEFPTLEVPLYAEQLFFELLQLGKTPVIVHPERNAHFRKDPNHMLPFLDMGCL
                     AQLTAPSYVGVFGKDIQKTAKMMVKHNLVQMAASDAHGVSKRTFYLKECYEQIAKDFG
                     KEKVVQMKQVAKDLINGDEISYPVYEEVKKKKFGLF"
     gene            70776..71465
                     /locus_tag="IWM35_000337"
     CDS             70776..71465
                     /locus_tag="IWM35_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HAP9918222.1"
                     /translation="MESEDLLSRISEVHPNQVWLDKEKVEKRYIYLFFKRMIDIVGSI
                     IGLILASPLMFYVAYRIRKEEPGSPIFFCHTRVGKNGKTFKMYKFRSMCLDAEEKLEE
                     LLDQNEIEGAMFKMKEDPRVTKIGKIIRAKSIDELPQLWNVLKGDMSLIGPRPPLERE
                     VAEYTRYDLQRLYVKPGCSGLWQVSDRNNVSFHKMVELDLEYISHRSLMYDLRLILKT
                     FYVIVKPNGAY"
     gene            71808..71981
                     /locus_tag="IWM35_000338"
     CDS             71808..71981
                     /locus_tag="IWM35_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1972 domain-containing protein"
                     /protein_id="HAP9918223.1"
                     /translation="MNRYVHGTVQKKNRKINGKLLVNPNGYEWLRAKGSYPVPKYCNY
                     SETLMEWYQLIIT"
     gene            73327..74577
                     /locus_tag="IWM35_000339"
     CDS             73327..74577
                     /locus_tag="IWM35_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HAP9918224.1"
                     /translation="MKKQYNIAVAGTGYVGLSIATLLAQHHKVTAVDIIPEKVELINN
                     RKSPIQDDYIEKYLAEKELNLTATLDAKAAYQSADFVVIAAPTNYNSRTQHFDTSAVE
                     AVIRLVMEVNPDAIMVIKSTIPVGYTASVRVKTGSKNIIFSPEFLRESKALYDNLYPS
                     RIIVGTDMDDERLVEDAHTFATLLQEGAIKENVDTLFMGFTEAEAVKLFANTYLALRV
                     SYFNELDTYAEMKGLNTQQIINGVCLDPRIGTHYNNPSFGYGGYCLPKDTKQLLANYA
                     DVPENLIEAIVESNRTRKDFIADRVLQMAGYYNYAEANQYDAGKEKPVVIGVYRLTMK
                     SNSDNFRQSSIQGVMKRVKAKGATVVIYEPTLKDGETFFGSKVVNDLEQFKAMCDSVI
                     ANRYDACLDDIKEKVYTRDIFQRD"
     gene            74596..75606
                     /locus_tag="IWM35_000340"
     CDS             74596..75606
                     /locus_tag="IWM35_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase"
                     /protein_id="HAP9918225.1"
                     /translation="MIITKTPFRMSFFGGGTDMEDYFKENGGAVLSTTFDKYCYVNVR
                     HLPRFFDYSAELSYSRTERVTSIEDIEHPAIRNAMKMLDMHEIRLTYEADLPARSGLG
                     TSSSFAVGMLNAFYALEGKYADKKKLADEAIYLERVLCNEAGGWQDQIAASYGGFNRI
                     NFNVDGYEVLPVIISPERKKQLNNNLMMFFTGFTRFSSDVQKANNVSGTEDKRVRLKK
                     MYELVDEAEAILTDKNRNLDDFGRQLDVTWRLKKGTGGAISTGSIDELYEKGMAAGAL
                     GGKLLGAGGGGFLVFYVQPEKQDAVRWAMRDLMYIPFEFENGGTRVIHYTPETYIEKQ
                     NG"
     gene            75616..76269
                     /locus_tag="IWM35_000341"
     CDS             75616..76269
                     /locus_tag="IWM35_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP9918226.1"
                     /translation="MLAEKLNKHIDLLIKRYPTLETIKQDIIDGYLIMEECYLNGGKL
                     LVAGNGGSAADSEHIAGELMKRFKTPRPVSKEFADKLVAIDPVRGPELAKNLECGLTA
                     IPLVAHEALSTAYINDVDGLGVFAQQLFGFGRSGDVFLGISTSGNSKNIMSATVVARA
                     LGIKVIGLTGAKGGELATVADVCVKAPEVETYMIQEYHLPIYHCWCLMLEDKFFGNE"
     gene            76288..77286
                     /locus_tag="IWM35_000342"
     CDS             76288..77286
                     /locus_tag="IWM35_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HAP9918227.1"
                     /translation="MKKTIMVTGGCGMIGSNLVKRLVKEGCWDVYVADNLWRGKLEYL
                     NDEDGHPVIDLDTHFFNADLTDYEQAKKVIGTTEYVVHLADVVAGIDYVFKNQGELFR
                     INNLINSNVFDCCRKAGKEKIKGVIYVGTVCSFPLTRQNTLNPEPLREEELFPALPES
                     AYGWSKLMGQLEMGYLEKETGIPCCILMLHNVYGTPTDFGERSQVIPALIRKAINNPE
                     EPFNVWGSGEQGRAFIHVNDIVNALVLALDKGWGHGHIQIGPSHCTSIKEIAYKIIEI
                     SGKDIKPFFDTSKPEGDKARCADYSKAKEILDWEPTVSLEEGLRESYNWIEKQIKG"
     gene            77362..78123
                     /locus_tag="IWM35_000343"
     CDS             77362..78123
                     /locus_tag="IWM35_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HAP9918228.1"
                     /translation="MEKNMYKRKRDFTVCGIVGVCATTKNEFVREVFSMPTSPVSATV
                     DLVGVPAIISAKENPKIAEMYSQATMAAIDGMPIVKIGRRKGFTCERCAAPDIMGMVF
                     EEGVRQGKTHYFYGGKDNEVLNKLRANLERDYPGIKIVGMYSPPFRPLNEEEDSKICE
                     EINNLNPDFLWVGIGAPKQEMWMWEHQKKIHNTVMFGVGAGFNFFAGTLEKAPEWMEK
                     ASLEWLFRLVKEPRRLWRRYILGGIKYIYYTIESK"
     gene            78141..79247
                     /locus_tag="IWM35_000344"
     CDS             78141..79247
                     /locus_tag="IWM35_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAP9918229.1"
                     /translation="MKILIVVTMTNNFGKKGSYNSQEIGLARGLAELGHEVIVYKSVK
                     SDEEVGTEQLSNTAMIHYIKCRYVGSNGFLSTKNFDKNADVLIQFADLQLSVPHVYSW
                     TVKNGIKYIPYNGITRSQSGNKYIKTIIEILAHRNYKIYKKNGCVAKNDRVSEKLVQL
                     GVNKVKLAPVCVDFEQLHKNYEAIDFTTLREKWNIPRNVRVILYVGRFEKDKRPLDMI
                     SLFNKLDEHNYLLMVGTGALLEQTKQQINLLGLQNRVKIIERIPNKDIWELYCLSDVF
                     VNLNYTEIWGMVLLEAMYYRLGIIAVNAPGPQNIILDNRYGMLAKSDDEILTFLKDGR
                     FTNDIIDAAYQRVTTEFRWSKSGEIMLSMLEGDK"
     gene            79669..80124
                     /locus_tag="IWM35_000345"
     CDS             79669..80124
                     /locus_tag="IWM35_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine acetyltransferase"
                     /protein_id="HAP9918230.1"
                     /translation="MNAYKLYKVERWFYLHKMEFFAKIVKALIYVLHNSSISYQTEIG
                     DNCKFLYGGIGCVIGKDTKIGNHVIIGTNVLTGGRSNKPGMPTIGNNVYIATGAKILG
                     NITIGDNVIVGANAVVINDVEPNCSVGGVPARVLKKDIDVNDYCSLNRY"
     gene            80138..80911
                     /locus_tag="IWM35_000346"
     CDS             80138..80911
                     /locus_tag="IWM35_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAP9918231.1"
                     /translation="MLYFVIPLMSAKAANNWEMVSTLFNRTLWSCYNQTDPDFKIIVA
                     CHEIPKLTHEYDDRVEFIQVSEKDAPIPTNQDEKMIDKGYKTHALAMRLRELGGGFAM
                     MVDADDLVSSHLAEYVNSHPQENGFYVKTGYVYFVGDDYMKVLPKFSSGSACIVKYAV
                     EDLPEAYPEVMTANCDDNPWIIRKRHGGVVPACEEQGRPLKPLPFKAAVYVLGTGDNH
                     SLYGKTTKYQTKMREILEMFIAKRKIKGKLKAEFSVDWL"
     gene            80898..82028
                     /locus_tag="IWM35_000347"
     CDS             80898..82028
                     /locus_tag="IWM35_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAP9918232.1"
                     /translation="MIGYKVSIMKVLMCESQMPENWYSFDLSKELNQYVDLTLLCKRD
                     VPPLDPKIKSKHILYHKSTNKVFSALVFLKSLFLIWKEIRFGDYDIVHLQLMKSLKYE
                     GGLYVLCKRHYKKLIMTVHNVLPHEVREKDRQEHFNIYSNCDGFIVHNRVTEQHLYAE
                     FPELNAKSYVCPHAIYGDVKHISRAYDKDKFEFILFGQIRPYKGIDVLLNAITELPMD
                     VRKKVHFTIAGQQHPKQDSTDYKKMIEDLKVSDCVLYKPERISDEEMDSMFRNADAAI
                     FPYKEIYGSGALLMAYTYCLPTIVSNVPAFMEETEEGKIGLLFENLNSKSLADTIVKY
                     VNLSPAEKSKIEDDIYELVNRKYTWAASAKLTYEAYKQILNE"
     gene            82059..83294
                     /locus_tag="IWM35_000348"
     CDS             82059..83294
                     /locus_tag="IWM35_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9918233.1"
                     /translation="MQISKWKIIQFLMVFLMIISSSILKPLGGWLGETMNVRLVTVIL
                     AIVCLLIASLPAFRKNRYLNFCTLLICVVIIAAYIYSDKTIWVKSRESMSLYYVLLAY
                     PLFCTLINQAWNLDSMLNTICGVTFLSYILRTSISLIQKFSGVLLYENIYRECAPENW
                     YRGGFLRINPPCFTIIIIPIALYMIERQVVARRKVKYYLLIASALAYSAVIHGSRSVL
                     VYQIIVLGCYILFKKGSSKRKIAMWVLAGLLVVIFINSTMFSTFVETFTNSTGEYAGS
                     AESRFASVWFFIPKFLQSPLFGTGILTGEEFTFILPGGVRGHLSDIGFFYTITQYGVG
                     ILIFDILFIIKGFKTALLASKVGMTGYQYLAFGITLNVLLTGINIDWFYPIFTPAIPV
                     CLAVIEYIYQECRSVANIE"
     gene            83319..84428
                     /locus_tag="IWM35_000349"
     CDS             83319..84428
                     /locus_tag="IWM35_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9918234.1"
                     /translation="MNKKEILYNILKKYENLPYVFRNMFGHNISKLVSWKLTADCATV
                     EENSEVYAAFQLRSKDMSDVPVMGLVNCCKDVAIVMQGPLCLKDDFSYNTLAYYKKCY
                     PNALIILSTWKDEDIKALKRIEDLGVIVICSEKPEKPGHLNINYQVGNTLPGIVRAKQ
                     LGAKYVCKTRTDQRIYHPNAMAFFCSLLEQYPVNNEDDWKLIQNQRLLLLSMPYGDMF
                     FPYCLSDFLYFGDVDDMINLFSIPSDVREKGAVSRGVSRREISENNLAPEVQLLRSYI
                     SRMGGNEECSIRAYWEFVKNHVITINKNQIDLYWHKYVGRYSNNTIYGTYYIDDSQDA
                     LHCYNFDFINWLNLYTGKYEYKAEYEKYMDFVWEE"
     gene            84470..85804
                     /gene="gmhB"
                     /locus_tag="IWM35_000350"
     CDS             84470..85804
                     /gene="gmhB"
                     /locus_tag="IWM35_000350"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HAP9918235.1"
                     /translation="MKTVIMAGGRGSRISELFPDIPKPMIPIDGIPVLERELCSLRDQ
                     GFNDIILTVSYLHEKIEEHFGDGSKLGIKIKYFIEKTPLGNAGALYFLLDELGDEPFL
                     LLNADAVFDVDFRQMIRFHVENNADVTLFTHPNSHPYDSGLIISDDNKKVLQWLAKED
                     QRPEYYKNRVNAGLHVINPKVLRENPLMEIVNKSIVEGGGLPYKIDLDRMILKPLANT
                     GRMYCYDSPEYVKDMGTPDRYRAVIKDFQKGIIVGKNLSNPQKAIFLDRDGTINKYVG
                     FLRNAEEFELIDGVSEAIKIINSLGFLCIVITNQPVIARGEVTVDELNSIHNKLETML
                     GHAGGFLNGIYYCPHHPHKGYAGEVIELKIDCNCRKPKPGLLLEASKDFNINLSQSFM
                     VGDSESDVIAGKNAGCKTVLITEDITLPANYGQDYSVCSLLEFVKNHLCTMQ"
     gene            85839..87476
                     /locus_tag="IWM35_000351"
     CDS             85839..87476
                     /locus_tag="IWM35_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular biosynthesis protein"
                     /protein_id="HAP9918236.1"
                     /translation="MKVLIPAAKVVEKELQSIGMLPAIIYPINKKIVFDYLYNQYNAI
                     CDSLDIICYEKADKVHRRLKNYHDSRIHICDLPELGDLGQTVYFGLSDEDEPVLINFA
                     DTIVMESMSDIEMDTFYYSEDFVSSTWTYFKINEGIITDIHDKEEAYVSDKREKLFIG
                     VFMFSSSKLLKSCLREAVESRKQGGINSFYRALQLYSERCPLKAVRTEKWFDIGHIDK
                     YYNTKLEVKAREFNHISIDKDRGILKKTSDDKDKFIGEIKWYLKLPADIEYVRPRIFD
                     YSTSYVAPYVDMEYYAYHTVHELYLYGDLKYNQWHDLFSRIKFICSDFKRYTLKDEKI
                     RESLEDMYLRKTMQRFQKLSEDKRFSAFFEKQIQINGRKFISLTGVIELLKNLIPSML
                     YDVNEFCIIHGDLCFANMMVDSNCSFVKVIDPRGKFGKYDIYGDSRYELAKLFHSLDG
                     KYDYIIKDLFTVEYDTECAVIDYTINTRVRDYDLFELFKSVFSDEIKDDFDKIVLIEA
                     LLFLSMIPLHGESFDHQIVMLATGMELLARVSNKVIL"
     gene            87578..87973
                     /locus_tag="IWM35_000352"
     CDS             87578..87973
                     /locus_tag="IWM35_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular biosynthesis protein"
                     /protein_id="HAP9918237.1"
                     /translation="MYSEYSFVFDIDGTLCPIKKKDERYEDLVPYENMVEKLRFYKEN
                     GAKITLFTSRNVNSYKGNLGMINANTAKVLLAWLDKWDIPYDEILYGKPWPGHKGFYV
                     DDRTVRPDEFLRCSVDELNEICNSSKKRF"
     gene            87991..88722
                     /locus_tag="IWM35_000353"
     CDS             87991..88722
                     /locus_tag="IWM35_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9918238.1"
                     /translation="MEKKMTVIITMAGLGSRFRKIGYAVPKYMINAKGRTLFDWAMDS
                     LVGYNKNVDKYIFVVRQEDNASAFIKEHCAYFGIENVEVIEIDYMTDGQATTCMLAIP
                     YCNQDNEIMVYNIDTYVESNEMKYEDICGDGHIPCFHADGDHWSFAKLNDRNEVIEVR
                     EKVRISDNCTLGAYYFSSAKLYKALYEEYYADSSNMEKNEKYIAPLYNFMIKKGLKVT
                     ISIVDAKKVHVLGTPEELNVFLNQE"
     gene            88746..90197
                     /locus_tag="IWM35_000354"
     CDS             88746..90197
                     /locus_tag="IWM35_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HAP9918239.1"
                     /translation="MTSNNLIVKTLSRYKNTPLPVKAALWFMVCGFLQRGVSMITTPI
                     FTRVLSTEQYGLYSTYLSWESLLLMITSLSLFKAMMNLYVKYDNQEHILSTVSGLTLV
                     LTIFWFVIAIFIKDILAEILGLTPVLVVCMFSYFIFQAEIQCWSLHKRYIYDYKTLLV
                     ATLAVTVGSSVVGCLAVLFISPTAEARALMNVLVTGVVGVAVYISVFRRCKDFKDNEV
                     WNFAVVFCVSLMPHYLSEFILNGSDKIMINYLCGSSDVAIYSVAYSIGALINLVTNSV
                     NSTFAPYQYQKIKSGEYDLLSKRANEVLGLVAAMLFALMLFSKEIVLIFGGQKYIDSV
                     NLIVPICIGIYFNYMFQLFARVQEYFEKKLTVVIPSVLCAIMNIVLNYVFIKLYGYQA
                     VAYTTVFSYMFFCVIHYIFYRKVCKELLDGKNIYDVRGILLISAITIAGGILISIINK
                     FDFIKYLIIAIACVAAIIFRKRLLLLVRRIMSK"
     gene            90223..90834
                     /locus_tag="IWM35_000355"
     CDS             90223..90834
                     /locus_tag="IWM35_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HAP9918240.1"
                     /translation="MIDIISAITVNVLTALYQPFLFAVLTAYLFMYWYLFIVDNSRDP
                     KNLKDSVLIWLRYFKASSKFRRLFFLAFYFTLILFKTLLNRNMWMNPLSNVIGQWSLY
                     IIDNATGESRLTTECVENTMLFIPFCMLLLWWRDIKCDIIRTIYVGIKYVFLFSLSIE
                     FTQLFFRLGTFQLSDLFYNTLGGLIGALLYWLFYRLNKYIDLK"
     gene            91016..91531
                     /locus_tag="IWM35_000356"
     CDS             91016..91531
                     /locus_tag="IWM35_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-keto-6-deoxy-D-glucose epimerase"
                     /protein_id="HAP9918241.1"
                     /translation="MVVVMVKVFDIIKFSDFRGYTAVALDNETLFSIGIEFHIKQINQ
                     GFSHNKYTMRGLHYQSGEHAQGKIVMCLHGEIMNIALDIRQGSSTYGCVYVNTLSESN
                     GKCAYIPRGYAHGYLTMEDNTLVQWCVDNDFNSNAAKCISYESVDIEWPVSRQCFVIS
                     EKDKNVERLSF"
     gene            complement(91666..>91843)
                     /locus_tag="IWM35_000357"
     CDS             complement(91666..>91843)
                     /locus_tag="IWM35_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HAP9918242.1"
                     /translation="KTVETVFSSLEKLGCQNFNSRSVKGLESRFESILLAYSVLLSRA
                     QRRFEGTLRYSLGY"
BASE COUNT        30017 a        15498 c        19674 g        26654 t
ORIGIN      
        1 aagtagacga agcagctgaa aagttatatg atttggtcaa atcagaaaaa tacaatattg
       61 aagtgaatat tccgaaaaaa ggcggaaaag cggtccaggt aaaatcaaaa cgcccaacga
      121 atcatacaaa aaaatggatg gctaaaaacc gctaataata acaaaagatc cataagtcgg
      181 cattttcatc gattttaggg tcttttgtta ttttctaggt tgaacatatc taaaaaattg
      241 tttctgacaa taggagtagg aagaacgatg tgtgtctaaa tcctctgttg atggatagaa
      301 acctttatct ttttcagttg tgaatcaggt atataccagt tataatagaa gaaacgaaag
      361 gacgggtgta cgttatgtcg aatcaattac ctgtatatat acagattcat gaccaattaa
      421 aaaatgaaat cgaacaaggt atatggaaag tcggagaccg tcttccttca gaaagagaat
      481 tagcagtgaa attcaatgtc agccgtatga cgttgagaca agcaattcaa aacttggcag
      541 atgaaggaat cttagaaaga aagatcggtt cgggtacata tgttgcgcga gaaaaagtac
      601 aagaaaaaat gtctggtgca acaagtttta cagaaattat ggaatcgcaa aatcgagtac
      661 cgtctagtcg gacaatctct tatttcttga cttcgccaag ctcaagtgaa atggaaaaac
      721 taaacttgag taaagatgac acgatcttgc gaatggaacg tattcgtttc gcagatgatg
      781 tccctatttg ttttgaagtt gccagtgtgc cacaaaagct gatcgatggc tacagcaaag
      841 cagagattac tgaatcgttg tatcgcacgc tagaagaaaa aggcgggaaa aagatcggtc
      901 cagcgaatca aacgattact gctgtgttgg cttctgagaa aattgctgac tatttagagg
      961 taaaacgagg agatgcagtt cttcgtttgc gccagatttc ttattttgaa gacggtacgc
     1021 cgtttgagta tgtgcgtacg caatacgtag gaaatcgttt tgagttttat ttggaaaaat
     1081 aaaaaacagg tgatccctga ttccttactt agaaataaaa gagaatcaga atcacctgtt
     1141 tttatatatg atcagttacc agttgctcgt gataagaaac aggaccctct tcaaattgga
     1201 cttgtccgtt cagcagctca atgatggatt ctttaaagtg gtcagcactt ttttcatcta
     1261 cagcacagat gacgctaact tgatcagtat attggatttc ttttataaaa aaatgttgga
     1321 gttcaagata attctgtaat tttcctagtt gcggatacgt gatcatcaca gagatctcct
     1381 gttgcaaggt accttctacg attccaatgt cagacaatgc ttgagagaca gcatgagtgt
     1441 atgctcgtat taatccgcct gtgccgagtt tcgttcctcc aaaatatcga gtgactactg
     1501 cagctacatt gatcagttga ttttttttga ggacttccaa catcggtacg ccagccgttc
     1561 cacttggttc tccatcatcg ctgcttcgct ggatttcatt tttttctcca atgatgaagg
     1621 cgctgcagtt atggttcgct ttccaatgtt ctttttttat ttcttgaatg aaactttttg
     1681 cttggtcctc tgattctatt cgtctaagag aacagataaa gcgtgatttt ttgatctcaa
     1741 tctcactttg tccattttct tttattgtac gatagcgctc gatcatcttg tcacctcttt
     1801 ttttcatttt ctttattcag tataaataaa gttctaggtt gtttcaacac tgtaacggtt
     1861 gcgcaatcgc tgtagtatgc gataataaag acaaaggtag gtggatctgg ggatgcagga
     1921 acgaatgaaa aagtacgatg caattacgca ttatttgaag aacaatggcg gctctcaagt
     1981 aacgttgacc tttacccaat ttgacgaact gctgtttccc agtaacggac ttcccaaaac
     2041 agcaagggaa agtacagatt ggtgggcaaa tgattataaa caccctgaaa aaggtgccta
     2101 tggctggata aatgcaggct atgaagtagt tgtcatcaat ttggacaaag aatatgtcgt
     2161 gttcaataaa ttagtgaaat ctagttggct atttgattaa ataaaccgcg tttagaaaac
     2221 gttgacaaat ttctttcttt ttatataata gagtaacaga cgcgaagaaa acgtttgcgt
     2281 agtttgtttt aaaaaggaga gaaaagtatg gctaaaaaaa caatcatgct tgtatgttcg
     2341 gctggtatga gcacaagttt actagtaaca aaaatgcaaa aagctgctga agcaaaagga
     2401 atcgactcgg atattttcgc tgtgtccgct tctgacgcag ataacaatct tgcaaataaa
     2461 gatgtagatg tactgttatt aggacctcaa gttcgcttta tgaaagctga ttttgaaaaa
     2521 cgtctggaac caaaaggcat tcctttagac gtcatcaata tggctgatta tggtatgatg
     2581 aatggcgaaa aagtgttaga tcaagcaatc acgctcatag gtgaataaat agaacagtat
     2641 aactatcgaa caagcgatcc aaaaaataga tcgcttgttt tttcgtatat ttttttaggg
     2701 cgaataatat gaatcatttt tggggtagaa ggcttctgac gagagatatt aaagcaatag
     2761 actgtgagga ggtaaattta ccaaattatg agaagatcgc agcagtggaa cgtttgggca
     2821 actggattct ttgtcatcgc tgtggaatga ggacgtccgt cattgatgga cagcttgcag
     2881 attacggtta tttttgtaca cattgtctat atttagggcg atgcgacact caacaagatt
     2941 tatatttgtt tgatcagcct gaagcaaaac ctcgagaagt cgtctttttt tggacaggac
     3001 agcttactga aaaacaaacg gaaatcgcaa agagaatact ttgccattca gaaaaaagac
     3061 atcatcttat ttgggctgta acaggtgcag ggaaaacaga gatgctttat cctattttag
     3121 tggaaacttt aaaagctgga ggaagagtag cgatttgtac accgcgagtc gatgtttgta
     3181 atgaattgtt tttacgctat cgttatgttt ttccagaaga aaaaatcacg ttgctccatg
     3241 ggaatacagc aacggcttat acctttactt cttttgttat ctgtacgatt catcagctat
     3301 accgttttta tcggtccttt gatttaatag tgatcgatga gatcgatgcg tttccttata
     3361 acggggatgc aggattagct catgctgtgc aaacagcgat taaaccagac ggacgattca
     3421 tctacttaag tgccacacct gataaaaagt tactaaagga aatcaagcag acgtttgaca
     3481 tccacaaact tgctttgcgc tttcatcggc gtttacttcc tgaacccgtc ttggttttca
     3541 tgaataattg gcgacaaaaa tgcaagaaca agaagaagat aaaatctttg gtgcggatca
     3601 ttcatcaatt aattaaagaa aaccatatcc ttttattttg tccaagtatc acgatgatgc
     3661 atcgtttgaa agccacactt caagaggctc ttccgagata tgagatcaca gatgtttctt
     3721 caagagacaa tcagcgggcg gaaaaagtcc agaaaatgcg agaagcaaaa tatgacatac
     3781 tgttaaccac gatgatcctt gaacgtggag tcacatttga aagaatatcc gttatcgttt
     3841 taggagcgga tcatccggtt tttactaaat cttctttggt acaaatcgca ggaagagccg
     3901 atcgtaaagg gccttatacg aacagtcaag tatacttttt ctatgaagaa aaaactgcag
     3961 ctattcgaaa agcttgtcag gaaatcaaag aaatgaatga agaagggaag aagatgctgt
     4021 gaggtgcagt tgctgcagta aagatatcgt ccaaaattta acattcagcg agattattgg
     4081 tgtttcgaaa tacagattac ctcagacttg ttcagtttgt cgcgcacaat ttcatttatt
     4141 ggaaacaaga ggctgttctg gctgtcaaaa accaatccat ttcaatctag agagtacgaa
     4201 aaagaaagta aagtctattc ccgtttactg cagtgactgt caagaatgga agaagaaata
     4261 tccgacctat gattttcagc accgtgcctt ttttgtgtac gatgaaaata tgaaggcctg
     4321 gctcaatcaa tacaagttta tgggagatat ccgactagca ggcacctttg catataaatg
     4381 gacgcagata aaaaaagaat acccgacata catctattgt ccaatacctt tatctgaaaa
     4441 aaggcgaaca gaaagaggat tcaatcaagt aagcgagatg ttgaaagcag caagaattcc
     4501 ttttcagatg ctactcaaaa gagagaagca tctgctggct caagcaaaaa acacgcgaaa
     4561 ggaaagattg gagatgcctc aaccttttga acttgcagtc accttttcta agatacaaaa
     4621 taaaaaaata ttgattattg atgatgtcta tacaacagga cagactcttt tccacgcagc
     4681 agattgtttg caggtcgcac agccgaaaat catccgaacc atttctttag caagatgaat
     4741 tagatgtgga aatttgatag cgatttaatt tgcaaataac ctagagttcg atataataga
     4801 attaagaaga gggaaagagt ggtccttctc cttcttgatt gtggtagacg aaaggggtaa
     4861 tttttatgtt tagatataat gtacgcggcg aaaatatcga agtaactgaa gcgattcgcg
     4921 actacgttga gaaaaaagta ggcaaactag aacgctattt cagtgacgca cccgatgcaa
     4981 cggcgcatgt gaacttaaaa gtttatacag aaaaaacagc aaaagtggaa gtaacgatcc
     5041 cattacctta tcttgtatta cgtgccgaag aaacttctcc agatttatac gcaagtatcg
     5101 atttggttgt tgacaaattg gaacgccaaa tccgcaaatt caaaacaaaa attaatcgta
     5161 aatcccgtga aacgggcatg aatactgcag atgctactgc tttattcaat gaagaggacg
     5221 aaaacgaaaa tgattctgaa ttagatatcg tacgtacgaa acgcctttca ttgaaaccaa
     5281 tggatagtga agaagcagtc ttacaaatga acatgctggg acataacttc tttatttttg
     5341 aagatgcaga aacaaatggt acaagtatcg tttaccgccg taaagatgga aaatacggac
     5401 tgattgaaac agactgatca gccttactta aataacaacg aaaacttgca gcagctactg
     5461 acagctgccg caagtttttt tgagtttcct cctcttttag atgaaatatg aataaattat
     5521 gaagttctta ctttaatacg cttaagatat ttcaattatc tataactaat gataaaatga
     5581 aaagtaaagt ctacatagtc tggataatac acagaaggaa aaggagatta cacaacgcat
     5641 ggccaatttg attcgttcac ttatcgaaaa cgataagaaa gaactaaaac gtttagatag
     5701 cattgcaaaa aaagttgaag catacgctga ccaaatggca gcattaactg atgaacaatt
     5761 acaggcaaaa actgctgaat tcaagggcag ataccaaaaa ggagaaacat tagatcaatt
     5821 acttccggaa gcttttgcag tcgtccgaga agcagcaaaa cgtgtattgg ggttatatcc
     5881 acaccacgta caattgatgg gtggaatcgt gcttcacgat ggaaatatcc ctgaaatgcg
     5941 tactggtgaa ggtaaaacct tgaccgcaac gatgccagta tatctaaatg ctctgactgg
     6001 cgaaggggta cacgtagtta ccgtcaatga atatttagca acccgtgact cgacggaaat
     6061 gggcgaactt tataatttct taggattaac agtggggttg aatatcaact caaaatcttc
     6121 ggatgaaaaa cgcgaggctt ataattgtga tatcacttat agtacgaata atgaattagg
     6181 atttgactac ttgagagaca atatggtcgt ttaccgccat caaatggtac aacgtccgct
     6241 gaattatgcc attgtcgatg aagttgactc aatcttgatt gatgaagcga gaacgccgct
     6301 gatcatttca gggcaagcag aaaaatccac agctttatat acaagagcag acaacttcgt
     6361 gaaacgtttg aaagaagaag aagattataa aatcgatgta caatcaaaaa caatcagttt
     6421 aaccgaagca gggatcgaaa aagcagaaga aacattcggt ttggataact tatacgacat
     6481 tgaaaatact gcattgaccc atcacttgga tcaagcattg agagccaact atatcatgct
     6541 tttagatatc gattatgttg tccaagacgg aaaagtactg attgttgacc agttcactgg
     6601 tcggatcatg gatggacgcc gttattcaga tggacttcac caagcaatcg aagctaaaga
     6661 aggcgtagag atcgaagacg aaacgaaaac catggcgacg atcactttcc agaactattt
     6721 ccgtatgtac aagaaacttg cgggaatgac tggtacagct aaaacagaag aagaagaatt
     6781 cagagaaatt tataacatcc aagtatacca gattccgaca aatcgtccgg ttatccgtga
     6841 tgatcgtcca gatttgcttt acccaacatt acaaagcaag ttccacgctg tcgtgcaaga
     6901 tatcaaagat cgctaccgca aaggtcaacc tgtgttagtc ggaactgttg cggttgaaac
     6961 atctgagtta ttatcagata tgctgaatca agaacgtatt cctcatgaag tgctgaatgc
     7021 gaaaaatcac tttaaagaag cagagatcat catgaatgct ggtcaaaaag gtgctgtgac
     7081 gattgctacc aacatggctg gtcggggaac agatatcaag ttaggacttg gggtaagaga
     7141 acttggtggt ctagcagtta tcgggacaga acgacatgaa tctcgtcgta tcgataacca
     7201 gcttcgagga cgtgcaggac gtcaaggaga ccctggtatg tcacagttct acttgtcatt
     7261 ggaagatgat ttgatgaaac gattcggttc agaacgaatc aaatctttct tagatcggat
     7321 gaagatcggt gaggaagatg ctgttatcca aagcaagatg ctgacgaaac aagtagaatc
     7381 tgcacaaaaa cgtgtagaag gaaataacta tgacacacgt aaaaacgtct tacaatacga
     7441 tgatgtgatg cgtgaacaac gtgaagtaat ctataaacag cgacaagaag taatcatgga
     7501 agaaaaaagc ttatccaagc cactaatgaa catggtcaaa cggacaatca gtcgtgtagt
     7561 agatgcgcat acacaattag agaaagaaaa ttggaatttg gaaagtatca tcgattttgc
     7621 cggcagtact cttgtccatg aagatagtat tactcttgca gatatcgaag gaaaaacagc
     7681 tcaggagatc aaagagtatc taaacgaacg agcacaagaa gtatttaata ccaaagcggc
     7741 tcaactaaat ggtccagagc aactgttgga atttgaaaaa gtcgtgatct tgcgtgttgt
     7801 ggactctaaa tggacagacc atattgatgc aatggatcaa ttacgtcagt caatcggctt
     7861 acgtgcttat ggacagaaca atccattagt cgaatatcaa acagaaggct ataagatgtt
     7921 tgaagatatg gtaggcgcca tcgagtatga agtcactcga atctttatga aagctgagat
     7981 ccgtcaaaac gtacagcgtg aacaggtttc tcaaggaaca gcttctcact cagaagacgg
     8041 agatgccaaa gaaaatagta atactagcgc gaaaaaacag ccaatccatg tgaacaaggt
     8101 cggaagaaat gatccttgtc cttgtggaag cgggaaaaaa tataagaact gtcatggaaa
     8161 aaatgcaaac taatttctga cagcaggatt ggtgagacaa attctttttt taagaattcg
     8221 tctcaccttc tttctgttat ttgctgattc tgttagacta gatataatga aaaaagtata
     8281 gtgaaagaag gaaaaaacat ggaaatatcc gaaattcgaa atctcctgac agaaatgcag
     8341 gaaaaggtca caagcttcag gaggtctctt tgacttagac cagctggaag aagacattgc
     8401 ggaagcagaa gcaaaaatgg ctgaacctgg tttttgggat gatagcgaag ctgctcaaaa
     8461 agtgatcagt gaaaacaatg caaataaaga aacttatgat caattccatt ctcttgctga
     8521 agaactggaa gaaatcgaat tgatggtgga gatgttgcag gaagaacctg atgcagccat
     8581 gcaagaagaa gcagaaacac gtatcaatgc gctagatgaa aaaatgaaga catatgaatt
     8641 atctatgttg ttagatggtc cttatgatcg aaacaacgcc atcgttgaat tgcatccagg
     8701 ggctggcgga acggagtccc aagattgggg cagtatgctt ttacgtatgt atacacgctg
     8761 ggcagagcag catgggtatc aagtagagac actagattat caagcaggtg atgaagcagg
     8821 aatcaagagt gtgaccctac tgatcaaagg acacaacgca tttggttatc tgaaatcaga
     8881 aaaaggtgtt catcgccttg tacgtatctc gccttttgac tcagcgaaac gacgtcatac
     8941 ttctttttgt tcggtagatg tgatgccaga gcttgatgaa acgatcgata tagaaatcaa
     9001 tccagatgat ttgaaaatcg atacgtaccg agcaagtggt gcaggtggtc agcacatcaa
     9061 taaaacagaa tctgctgtac ggatcactca cttgcctaca ggaacagtag tagctagtca
     9121 ggcacaacgt tcacaattga aaaaccgtga gcaagcgatg gggatgttaa aagctaagct
     9181 ttatcaatta gaagtggaaa agaaagaaca agaagcagct gcacttcgag gagaacaaaa
     9241 agaaatcggc tggggttcac agatccgttc ttatgttttc catccatatt ctatggtaaa
     9301 agaccatcgg acaaacgtag agacaggaaa tgtccaagca gttatggacg gtgaccttga
     9361 tttatttatt gacggctatt taaaaatgca tctacaataa gaacccattg ttaaattgaa
     9421 ataaaattgt aagattttgc aagcatgttg aaacattgcc atgctataat agctaaagac
     9481 tgaaaaagat atggagagaa cgccatgatt gaaatgaagg atgtaatgaa gaagtactca
     9541 aatggtacga ccgctatccg caatctctca attgtcatcg aacaaggaga gtttgtctat
     9601 gtggttggtc cgtctggagc cggtaaatct acattcatca aattgatgta tcgagaagaa
     9661 aaagcttcaa aaggaacttt gaacgtcgca ggtcatgatc taatagcaat caaaaaccgc
     9721 gaagttcctt atctacgcag agaaatcggc gtggtgttcc aagactataa attattacca
     9781 agaaaaacag tatatgaaaa tgttgcgtac gctatgcaag ttatcggcag aaaacctcgg
     9841 gatatcaaaa aacgagtaat ggaagtgctg gaccttgttg gtttgaaaca caaagttcga
     9901 gtctttccta gcgaattatc cggtggggaa cagcaacgtg tctctattgc tcgggcaata
     9961 gtgaatacgc ctaaagtact tatcgcggat gaaccgacag gaaatctgga tccagaaaac
    10021 tcatgggaaa tcatgaaatt attagatcgg atcaatgccc aaggaactac tgtcgtgatg
    10081 gctacacata acagtacgat cgtaaatacg attcggcacc gagtaattgc tatcgaaaac
    10141 ggccgcatta ttcgagatca agcggaaggg gaatatggat acgatgatta gaaccttttt
    10201 ccgccattta ttagaaagta tcaagagttt gaaacgtaac ggctggatga cagttgcttc
    10261 agtcagtgcg gtaacgatca cattgacttt agtcggtgta ttcatggcag ttatcatgaa
    10321 cgcaactaaa ttagctcaag atatagaagg aaacgttgat gtctctgtat tcgtagatat
    10381 cggtacaaaa gacgaagaga tgaaaaagct agaaaaagaa ttgaatgact tagatcatgt
    10441 taaaaaagtt gaattttcaa gcaaagaaca ggaattgaaa aaaatccaaa acgaaatggg
    10501 tgattcctgg aaattgtttg aaggtgacaa taacccgtta tatgatgtgt atgtagtaag
    10561 tgctactgat ccttcttata caaaacaagt agcagaagaa gcagcagatt tgaagaatgt
    10621 ttctcgttct gattacggtg gagcttcctc agatcggatt ttccaaatcg ctggtgcagt
    10681 tcggacatgg ggattagttg cagcggcatt gctgctgttc gtagcgatgt ttctcatttc
    10741 gaacacgatt cggatcacga ttttgtctag acagcgtgaa attcaaatca tgcgtttagt
    10801 cggtgcaaaa aatggatata ttcgctggcc gtttttcctt gaaggtggct ggatcggtct
    10861 gatcggtgcg attcttccga ttttgatcat cacatttggt tatgcatggt ttttccaatt
    10921 gatcaatcca tcattattac gttcccatta ttcattgatt catccacaaa atatcgtttg
    10981 gaaaatcaat cttttaatgg tcggcattgg tgtgatcatt ggttcacttg gctcgatcat
    11041 ttctatgaga cgtttcttga aattctaata ccacaatata aataaaatga gaaagttgga
    11101 cagtttcgtt ccagctttct ttttttattg gctaggagaa tagaaggaag cggctttttt
    11161 ccctttactt taaaaaacaa cttgggtatt cttaagttgg tagaagaaga ggtgaagccg
    11221 tgaaaattat cgttgacaat ttttttacga tccttttgat tatgaatatt ttgctttcct
    11281 ttatcatcgt ttttcgagaa agaaaggaaa ctgctcaaac atgggcatgg gtgctcgttt
    11341 tgatgttcat tccagttgta ggatttattt tatatatttt tttaggaaga gggatttcca
    11401 aagagaaaat ctttgatttg aagatccaag ggaaaatcgg taagaacctg gaaattgaag
    11461 aagacaaaca ggcattgatg cgagggcttt atcctcatcc gcctactgga caagtagatg
    11521 tgaaacaatt gatctatatg ctcacggtat ttgaaagtac gctctatacg actaaaaatg
    11581 aaatcacttt atttacagat ggtcgtgaaa aattcgatgc gctgattgaa gatatcaagc
    11641 aggcagaaga ccatatccac ttccagtatt atatttatcg cagtgatgca ttaggtgaag
    11701 aagttcgaga tgcattgaca gaggcggcaa gacgaggggt aaaagtcaga gtccttcttg
    11761 atgcatgggg ttccacacaa gtttcttcca gcttttttca gaatttgaaa aaagcaggcg
    11821 gagaagtcgc atttttcttt cctctatttg ttccgtatat taatcctcgg atcaattatc
    11881 gcaatcatcg taaaatcgta gttattgatg ggaaaatcgg atatacaggt gggttcaatg
    11941 ttggaaatga atatctagga ttggtaaaga aatttggtta ttggcgagac aatcatttgc
    12001 gtatttacgg agaagcggtg tataccttgc aaaaccgttt tttgatggac tggaattctc
    12061 agcatacaaa agaagtaggc tattctccca aattttttcc gtccattcac tctactggtg
    12121 atatagctgt tcaaatcgtg acaagcggac cagataccga acatgaacaa atcaaaatga
    12181 cttatttgaa aatgatttcg ttagccaaac gggagatatt gatccaaacg ccttattaca
    12241 taccggatgg atcgattcat gaagcactga agttggctct tctttcaggc gtaaaagttc
    12301 atatccaaat acctaacaaa ccagatcatc ttctcgttta ctgggcaacg tattctttcg
    12361 ctgcagagct aatcgagtat ggagcaagga tcgaaacata tgagaatgga tttattcatg
    12421 caaagacgat gattatcgat ggtggcattg tttcagttgg ctcagcaaat atcgatgttc
    12481 gttcatttcg cttggacttt gaggtcaata cactggttta tgatgaacga atggcttcac
    12541 gtgtaagaaa agcttttttt gaagactcga aaatttctac gcatctcaca aaagaaatgt
    12601 atgaaaaccg aggaataatc atcaagatga aagaaggatt agcaagattg atttcgccgc
    12661 tcctttaaaa cataaaaagg acatgagaac catcaacaga tggagatctc gtgtcctttt
    12721 ctaacggggc catttctgag agtacgtcag aaatggagaa agcgtctcgc atgagcgaaa
    12781 ttaactttgg ggtatctctt gttttttggg gaaagcaaga aataagctaa tatatgctat
    12841 taaaagagac acatttcaaa gccagtatag catagaaact tttaatgaga tagttttttt
    12901 tacaattatg agaatttatt ttgttattta atgattttga ttcgttttca tcaagtaatg
    12961 aaaatgataa aataaacata ctttaggatt aaaggagatt tctaatgaaa aaacttaaat
    13021 tattcttacc aataatagga ttagctattc ttttatcagg ctgttcaaag tggattgatc
    13081 gaggagaatc cattacggct gttggctctt ctgctttaca gcctttagta gaaacagttg
    13141 ctgaaactta ccagacagaa aatcctggaa aattcatcaa tgtacaaggg ggcggaagcg
    13201 gaactggttt gtctcaagta cagtcaggag cagtcgacat cggaaactct gatttgtttg
    13261 cggaagaaaa atcggggatc gatgcctcag cattagtcga tcatcgagta gcagttgttg
    13321 ggatcactcc tattgtgaac aaggatattg gcgtaaaaga catcagtatg gaaaacctga
    13381 aaaaactatt tacaggcgag atcaaaaact ggagtgaagt cggtggaaaa gatcagccag
    13441 tagttatatt gaaccgcgct tcaggaagcg gaacccgtag cacgtttgaa aaatgggtat
    13501 tggacggaga aacagctgtt caggcacaag aacaagattc aagcggaatg gtgcgccaga
    13561 tcgttgccga cacacccggt gcaatcagct atgttgcttt ttcttatgtt tccgatgatg
    13621 tagatacgtt gaatgttaac ggtgtagaac cgacagatga aaatgtgaca acgaacaaat
    13681 ggacgatttg ggcatatgag catatgtaca caagagatca tccaaaaaaa ctgacaaaag
    13741 aatttttaga ctatatctta tctgatgaag tacaagataa catcgttgga gaactaggtt
    13801 atatccctgt ttctcatatg aaagtcgaac gtgattggca aggaaatatc gttaaagagt
    13861 aatctttaca caaaatttac aaacttagct aaacttttac atgttattaa aaccaaattg
    13921 atgttcaatt tgtacactga gtgagtatgt taaacctgat taagggggag tcatctgtgg
    13981 aagatgtgaa aaaagtatta ttaacaaaat caaaacgttc aaaaatggaa cagcgtggaa
    14041 aattcatcag ttttttatgt atcgcgctga tcgtgttggt tgtattatct attttttatt
    14101 ttgtagctag taaaggattg gccacctttt ttactgataa gatcaatgta tttgattttc
    14161 tgtttggtac agattggaat ccaagccaaa tcggagcaaa tgggaaacca atggtagggg
    14221 cattgcctat gatcacaggt tcgtttattg tgaccttttt atctgctatc gttgccacgc
    14281 catttgccat tggtgcagca gtattcatga cggagatctc tcctaaaaac ggaacgaaga
    14341 tcttacagcc ggtcatcgag ctgcttgtag gtattccttc tgtcgtttat ggttttatcg
    14401 gtttatcagt tatcgtgcct tttgtcagaa gtatttttgg cgggactggg tttggtatct
    14461 tagctggtac atttgtgttg ttcgttatga tcttgccgac tgtcacaacg atgactgttg
    14521 atgcattaaa ggcagtgcca cggcactatc gggaagcttc attagcgctt ggtgccacaa
    14581 gatggcaaac aatctacaaa gtcgtgctac gagcagccgt gcccgggatt ttaacagcag
    14641 tcgtttttgg tatggctcgt gcgtttggtg aagccttagc tatccaaatg gtcatcggta
    14701 atgcagcatt gatgccaaca ggtcttacta ctcctgcttc tacattgacg tccatcttaa
    14761 caatgggaat cggaaataca atcatgggaa cagttgaaaa taatgtgtta tggtcattag
    14821 cgctgatctt gctattgatg tcattattct ttaatatcgt gatccgtatg attgggaaga
    14881 aaggagagct gaaataatga acgcaaaacg ctcagataaa attgctacag gtgttttata
    14941 tatagtttcg gggatcatcg tattgatttt agcagcttta ctactttata tcttagttcg
    15001 gggaataccg cacatttcat ggtccttctt gaccgaacct tccaaagcct accaagaagg
    15061 cggtggaatc gggatccagc tatttaactc gatttatttg cttttgatca cgatgatcat
    15121 cagttttcct atttctttag gtgcgggtat ctatctttca gaatatgctg gaaaaaactg
    15181 gctaacagat gtgattcgca cgtcgattga aatcttgagc tcgttgcctt ctgtcgttgt
    15241 cggtttattc ggtttcttgg ttttcgttat tcaattccag tatggtttct cgattatttc
    15301 tggtgcattg gctttgactg tattcaactt gcctcttttg actagaacag ttgaggagtc
    15361 tttacaagcg gtccattaca cacaacgaga agcaggattg gccttaggtt tgtcacgctg
    15421 ggaaacagtg ataaaagtgg tggttccaga agcgacacca gggattttga ctggtgtgat
    15481 cctaagttct ggaagaattt ttggtgaagc agctgcattg atctatacgg ctggacaaag
    15541 tgcacctgcg ttagatttta cgaactggaa tccattaagt gtttcaagcc cgatcagcat
    15601 tttccgtcaa gctgaaacat tagctgtcca tatttggaag atcaatacag aaggcacgat
    15661 gcctgatggt gtttctgttt cggctggcgc ttctgccgtg ttgattattg ctgttttact
    15721 gttcaacttc ggcgctcgtg cgattggcaa acgattatac cgaaaaatga cttcagccta
    15781 aggagaagaa gcaaaatgaa aaaatataac tggaatgaaa caaatgtcat caccatgcca
    15841 aaagagaaag aagtagcact atatacggat gatctacatg tctggtatgg cgacaatgaa
    15901 gcaatcaaag gagtggatct ggaatttgaa aaaaataaga tcactgcact gattggtcct
    15961 tccggctgcg gaaaatctac gtatcttcgt tcgttgaatc gaatgaatga tgggatcgct
    16021 aatacgaaaa tcactgggaa aatcatgtat caaggcgtag atattaatgc gcctcaagtg
    16081 gatgtttatg agatgcgcaa acggattggc atggtttttc aacgtcctaa tccgttcagc
    16141 aagtcgattt atgacaatat tacatttgct ttgaaacagc acggcgaaaa aaacaaaaag
    16201 aaattagatg aaatcgttga aacaagcttg aaacaagctg ccttgtggga ccaagtaaaa
    16261 gacagcttgg acaaaagtgc cttggcttta tctggcgggc aacagcaacg tttatgtatt
    16321 gctcgtgcga tcgcgatgaa acctgatatc ttgctgctag acgagccggc aagtgctttg
    16381 gaccctattt cgacaggaac ggttgaagaa acacttgtaa acttgaaaga agactatacg
    16441 atcatcatcg tgacgcataa catgcaacaa gcagctcgta tcagcgacta tactgccttt
    16501 ttctatttag gaaaagtaat ggaatatgac aaaactaaca agatatttac cagaccaaaa
    16561 attcaggcaa ccgaagatta tgtgtccggt cattttggtt aagacggagg cctcaacatg
    16621 acaaaagaga ttatttcatc taaagatctt catctgtatt atggaaagaa agaagcacta
    16681 aaagggatcg atctaagctt caatcaaggg gaaatcactg cgatgatcgg tccttctggt
    16741 tgcggaaaat ccacttatct gcgttctttg aaccggatga atgacctgat tccgaatgta
    16801 acgatcaccg gaagtgtttt atataaagac aaagacatct atggtccaaa aacagatgtt
    16861 gttgaactaa gaaaagaaat cggcatggta ttccagcagc caaacccatt tcctttttct
    16921 atctatgaaa atgtcatcta tggattgaaa ttaaaagggg aaaaagataa aaaagtcctt
    16981 gatcaagtag ttgaagaaag cctgaaggca gcgtctgttt gggatgatgt gaaagacaaa
    17041 ttgcataaaa gtgctttatc tttatccggt gggcagcagc agcgtgtatg tatcgcacgt
    17101 gttttggcag ttaatccgga aatcatctta ttggatgaac caacaagtgc gcttgatcct
    17161 gtttctactg ggaaaatcga aagtatgctg ttagaattaa gagagcggta tacgatgatc
    17221 atcgtgaccc ataatatgtc tcaagcttca cgtatttcag ataagacggc attcttttta
    17281 gatggtcact tgatcgaata caatgatacg aagaagatat tcatgaatcc agaaaaacaa
    17341 gagacagaag attatatttc aggtcgattt ggataaggag gaaacggaat gctacgtaca
    17401 caatttgaag aagaattgct gaatctccat aatcagttct acgaaatggg gatgatggtg
    17461 agcagcgccg tgcacaagtc agttcgtgca tacatcaaac acgataaaat cttagcccaa
    17521 gaagttatcg aaaacgatat aaatatcaat aacatggaaa cacgcttgga gaagaaaagt
    17581 tttgaaatga tcgcattgca acagccagtt acaactgatt tgcgtatgat cattacggtc
    17641 atgaaagcta gctcggattt agaacgtatg gccgatcatg cggtgtctgt tgctaaatcc
    17701 acgattcgcg taaaagggca gacgagaata ccggaaatcg aaaaagagat ctccgatatg
    17761 tccgattatg tgaaaaaaat ggtggacaac gtcctagttg cttatgtcaa aacagacgaa
    17821 aaagacgcac gtacgatcgc caatatggat caaagagtca atgactattt caatcgaatc
    17881 tataatgcaa caatcaaaaa tatgcaggaa aatcctgaga cagtcatcag tggaacagac
    17941 tacttgaatg ttgccagtta tctagaacgg atcggagact atgtgacaaa catctgtgaa
    18001 tggatcgtct acttggctac agggaaaatc actgagttaa atacgaacca caatgagatt
    18061 ttctaaactg aagctgtgat tatgccgttt gtttaggaaa agaggttgtg aaatcagcgg
    18121 ttgtgaaaaa gctgttctgc atcaagtaat aagaacagtc aaacaaaaaa tagctcctca
    18181 tatttttcgt ttggctggac ttattaccgc agatgcaagc ttttgaacac cgtttattcg
    18241 ggaaagggtt tgtgacaaaa catgtcacaa acccttttca tcatttcata gaaaggataa
    18301 agaatgcaaa aaaaaattag catcatcact atatttttta tttgtgtgct tacttcatgg
    18361 gttattcagc atcaagccgc tttatatgaa gtacctgtca tcgagataga aaaagtacga
    18421 ataaaagagg atcatcaatt gattacagga aaactgatga acacggatca aaaaggagaa
    18481 tcgatttatt tatcggttcc ttatcaaaaa aatcaaatag aaacagcaaa tataaaagcg
    18541 agacaaatgt atctagtcca aatgcatgga gatgaatggc agctagtagc aaaaaaaaat
    18601 gatggctggc ttttcttctt cacaagtatc ttcattggcg ccatgttgct tattggtgga
    18661 aaacagggtc tatttgcttt aggaagtctc atcatcaata tcgttttact tttacttttg
    18721 ttgctcatct ttactaaaac agaagggatt tctctattaa atatatctat cctgttcgtc
    18781 gttataggga tgattattag catattggct ttagatggat ggaatagaag cagtataatc
    18841 aaaatttcag ctgcagttac gagtgttttt ttagcatttt ttatttgtct tttgacaatg
    18901 aatcattgga aagaccaagg actgcgctat gaggaactga attatctgac acgaccgtat
    18961 cgttctgttt ttctttctag cgtactgatt ggaacagctg gaggaacact cgataccgtt
    19021 atcacagtca ttgcgacatt agaagaattg actaagcgac aaccagaaat aacagcgaaa
    19081 cagttatggc tttcaggaag gcaagtgggt cgagatgtga ttggctcgat gaccaatatc
    19141 cttttgttcg tctatatcag tgggtcagtt ccatcgcttc ttttatattt aggaaatcaa
    19201 tggtcgttca aagaaacgat agaacagcat ttatcattgg aatttttacg cgtcattgct
    19261 ggcagcttag gaatagtatt atcgatccct atctcggtcc tcttttttct gactgtaagg
    19321 aggctgaaac gatgaatgta cttattatac ttgttgggat tttagcaatc agcgtgctac
    19381 ttattggcgg tacgcaaggt atgtatattc ttttaggctt attcatcaat ttagggatct
    19441 ttttcctgtt gttattcggt tatcatcaga agtggccgat actggcattg tcggtcatag
    19501 gatttttatt gattgctgtg gttatcttat tttttatcaa cggttataat ctaaaaatgc
    19561 gtgcagcttt tgtgagtata ctgatctttt tattttgttt cctactattg cttcctatca
    19621 ctgatttttt agcaatccaa ggttttacat cggtagaact agaagaatta tctggcttag
    19681 ataagacttt agcgattgat tttagattac ttacgagatc gttgcttctg attagtctta
    19741 gtggtgctgt tttagatgcg agtgtagcaa tttctagtgg gacattcgaa gtatatcagg
    19801 caaatccgca attatctttt taccaattgc gccattctag tttcgctatt gctaaaaaaa
    19861 tattggcgtc aacagtaatg acacttcttt ttgcattcat gggaagttct ttagcgttga
    19921 tcctctggtt tattgattta gacattcctt ttcagcaagt gatcaatgaa aaatcatttg
    19981 ttctagaata tacgatggct attcttacca cattgtccgc actacttgtt cttcctttaa
    20041 cctgcgtgac tacagcatat ctctttacaa aaaagaaaga acaactcaag atggagtaaa
    20101 agttacaact taagtcctat gacattttta ctgaatcagt gcataatatg gatgtaagga
    20161 aaagcttgca aacaattttg cttaaggagg caatttccat gaaagaaaga gaacgtattt
    20221 tagatttagt gaaaaaagga atcctttcga ctgaagaagg cttagattta ttagaaagca
    20281 tggcaactga aaaagacgaa aagcaaatca aaaaagaagc agacaaggta actgctagtc
    20341 ataaagaaaa agatcaggca agtcaattga tcgataaact tgaaaacggc gaagaagaaa
    20401 tttctgagcc agttgatcca aaggaaagag aaagacaaga tcaagaaaac ttagaaaaga
    20461 tcttagatga actagcaact gaagcgaata aaacttcggc gcgtctagat gaagtaaatg
    20521 ctcaattcgc tgacaataag gcagcacgca atgagaaaca ggaagcattg atgcagctga
    20581 atacaaaaga agaattaggc gagttgacag aagaagaatt agcacagcgt cagacgttag
    20641 aagcagaaat caaagaatta gaagctagtg gcgatacact gttagaagaa caattgaaat
    20701 tagaagcaga attaaaagat atccgtaaga atcaatggtc tgagaaaaaa gaaacgttta
    20761 ctgataagtt tgaattgcca gatgattgga aagatcaagc aacagataca ctgaatcaag
    20821 taggcgaaaa aatgtcagaa gctggatcac agttaggcaa attcttgaag aaaacattcc
    20881 aaactgtttc agaaacagtg aatgacaaca tggaatggaa agatgtcagc ttacgtgtcc
    20941 ctgggattgc gacaacgaaa tttgagcatg aattttacta tgaagctcct gcagcaagta
    21001 tccttgatat caaggcagca aacggaaacg tgacgttaaa aacgtgggac agcaacgatg
    21061 tgaaagttga agctaagatc aaactttacg gaaaaatggg tgctgaacca tttgaagcat
    21121 tctctgaacg gagccagatt gaggttaatg aagatcatat ctccttccaa attccaaata
    21181 aacgtgtgcg tgcagacctt gttttctact tacctaaacg agtatacgac cacgcagcta
    21241 ttaaacttct aaatggcaac atcatgattg aaacattgga agctaaagat atttatacaa
    21301 aatctacgaa tggtaacatc atcgtggacc aattaactgc aacgatgctg gaagtagaag
    21361 gtgtaaacgg caatatcgat attcgaaacg ggaacatctt agattctatt atcgaaacag
    21421 taaatggtac agtgacattt ggtgcgacag cagaaaacct ttctgtgtct ttggtaaatg
    21481 gtgatgtacg tcttacgatt aaagaagata atttgaaaaa agtagaagca agttcagtga
    21541 acggaaatgt caaagtcgct ttaccagatg gcatcggttt ggaaggacat gctaaaacaa
    21601 gtcttggaag tatcaacagc cgtctgtcca attacgaagt agttcgtgag aaaaaagaac
    21661 ggacaaacca aatgttgcaa ttccgtcgtg tttcagatgg tgaaatcgcg caagtgcaac
    21721 tttcaacgac aacaggaagt atctatttaa aagatacaga taaataaaga ggttgtgaaa
    21781 gagatgttca gccttgagca ataagaagcg attttgtaaa atagcttccc ctattttaca
    21841 aaattgtgcc ttattgccga tagtctatct cttgaacacc gtttaccaag agattatgaa
    21901 cctgccaatt atcgccgagt cacaaggaat aatttcagaa aaagctcgtc acgttttatg
    21961 aatatggtaa tttgtgaccg agaattttgc agaaagacac cgattaagaa gaaggttgtc
    22021 acaacgattt tcttcattta aaatagtaga acagaggtga gacatgatga aaaaaaagtt
    22081 gacaaaatct aataaaaatg ttgttttgac agggacttta gcaggaatag gagaatacat
    22141 cgggattgat ccgactgtta tccgtgtgct ttatgtattc ctgagcttag ttgcttttgg
    22201 aagtccaatt attttataca ttttattagc tttgattatc ccatctgcgc gttctgcacg
    22261 aacaaactat ggacataaca atgcatatta tgaaaaaaac aattatcgtg aacaaactcg
    22321 tgaacaaaca aaaaaacgca aagaagcgga aaaaatcgat gatgacgaat ggagtgactt
    22381 ttaatgtcgt actttcaacg tattttagtt aatacgttaa cattcatatc attggctgtt
    22441 ctttttcctg cgatggtcca tgtgagcagt atttggacgg ccgttcttgc cagcttcgta
    22501 ttgtctgtgt tgaatatgtt tgtcaaaccg attttgacga tactgtccct gccctttaca
    22561 ttagtcacat tgggattgtt tacgtttgtt atcaatgcag cgattcttaa aatgacctcc
    22621 ttttttgtgg gagcagaaca ttttagtttt tcttcattcg gtgcagcgtt gttagtagct
    22681 atcttgatgt cgcttgtcaa tgtaattgtc agcgaacata acagtagaaa ataaaagaaa
    22741 gctgtgccga actatcggcg cagttttttt atttagaatc aggaatgcgt tttctattac
    22801 attggtaatt aaataaaaac gtttttttta attggtagta cttttggcta gacaatgata
    22861 aaatgataga gaacactcta atagaaatga ggtagcatga atgtcagaag ttgtaaaagt
    22921 tagccaatta gtgaaaaaat tatctttgga aatcgtgaca ggcgacgaga aaagcttaga
    22981 ccgtgtcatc gtaactgggg acatctcacg cccaggacta gagttgactg gttattttaa
    23041 ttattattcc cataatcgtt tacagctatt tggcagtaaa gaaatcagtt ttgcggaacg
    23101 gatgatccca gaagagcgtt tgatcatcat gagaagatta tgtagtgaag atatgcccgc
    23161 atttattatt tcaagaggat taccagctcc agaagaaatg atccaggcag caaatgaaaa
    23221 tgggatagct gtactgcgtt ctcccatctc aacttctcgt ttattaggag aattatccag
    23281 ttatttagat ggtcgccttg cccctcgaac aagtgtccac ggtgttttag tggatgttta
    23341 cggattaggt gtactgattc aaggtgacag tgggatagga aaaagtgaaa cagcgttgga
    23401 gttgatcaaa cgcggccatc gattgatcgc agacgatcga gtagatgttt atcagcaaga
    23461 tgaattaacg gtgatcggag aaccgcctaa aatcctggaa catctgattg aaatccgagg
    23521 aatcgggatc attgatgtaa tgaatctttt tggtgcgagt gcggtacgtg gctctatgca
    23581 agtccagttg gctgtttatc tggaagcatg ggcaaaagat aagaaatacg accgtctggg
    23641 ctctgaagac acgaccgttg aaatcgcgga agtgggcatt ccacaaataa aaatccctgt
    23701 caaaacagga cgaaacgttg caatcatcat tgaagtagcc gcgatgaatt tccgcgcacg
    23761 tacaatgggc tttgacgcaa cgaaaacatt tgaagaaaga ttaagtcgct tgattgaaga
    23821 aaactcaggc aacgactagc aaggagtgac aatatgttag cacaaatcaa tcgagttgct
    23881 tttgagcttg tagggattcc tatctactgg tatgcattga tcatcgtatc aggtatcatt
    23941 atcgctatgt ggcttagcag cagagaggct gttcgggtag gattaaaagc agatgatgtg
    24001 acagatttta tgttgtgggg attgcctatc tccattattg gcgctcgctt atattatgtt
    24061 ttatttgatc ttccccagta tatagctgat ccaatccaaa tattcaatat ccgttcagga
    24121 ggcttagcta tttatggcgg actgattgca ggcgcgatca cgctgtattt ttttactaaa
    24181 tatcatttta tttctttgtg gactttttta gatatcgctg caccaagtgt attattagca
    24241 caggcaatcg gtcgttgggg aaatttcatg aaccacgaag catatggacc agacacgacg
    24301 cgaagcttct tagaaagtct gcatttaccg caattcatca ttgataatat gtatatcgaa
    24361 gggatctatc gtcaaccaac atttctttat gaatcacttt ggagtttgat tggatttatt
    24421 ttgcttttgt ttttacgaaa gaaaccacac cttttgaaaa aaggcgaagt ccttttatgt
    24481 tatctaatgt ggtatagttt cgggcgtttc tttattgaag gattacgtac agacagtctt
    24541 tactttttag gtgtgatccg tatttctcaa ttattatctg ctgtgttatt tgtcggtaca
    24601 ttgattctat ttatttggcg cagaaaaaaa ggaaatttac cggattatga ccgctcatta
    24661 ggaaaaaatc aacaaattat ctgattttta gagcgttaaa aatgaaaaaa atttcaggag
    24721 tggtaaaatg aattatagag tagcggtttt aggaccaggc tcttggggta ctgctttggc
    24781 acaagtcctc gccgaaaacg gacatgacgt tcggatttgg ggacatcgtg cttcacaagt
    24841 agaagaaatc aacacccaac acacgaatca acgttattta ccagatcatt atctacccga
    24901 ttccatccaa gcattcacca atatggaaga tgcagtaaaa gacgcagatg cggtgctatt
    24961 cgtcattcca acgaaagcaa tacgttctgt tgcaaaagaa ctagtgccta aattgaatac
    25021 aaagccagtg atcatccatg caagtaaagg attagaacag ggaacacata aaaggatctc
    25081 tgacatcttg ttagaggaaa tcccagcaga aaaaagaaaa gctgttgttg ttctttctgg
    25141 accaagccat gcagaagaag tagcaaaaca tgacatcact acgatcacag ctgcaagtac
    25201 agatgcaaaa gcagcaaaat atgtacaaga gctgttcatg aataattatt ttcggatcta
    25261 taccaatcct gatgtgatcg gtgtagagat gggggcagct ttgaaaaata ttattgctat
    25321 tggtgctggc gctattcacg gcttagggtt tggcgataat gcaaaggcag caatcatgac
    25381 gcgcggatta gctgagatca gccgtttagg tgtagcaatg ggtgccaacc cgctgacctt
    25441 tatcggatta agcggaatag gcgatctagt cgttacttgc acaagtgttc attctaggaa
    25501 ctggcgcgca ggcaacttgt taggacaagg gcagccccta gaagaggtgc tagccaatat
    25561 gggaatggtc gttgaaggtg tgaatactac ccaagcagcg atggaactag cccaaatgat
    25621 ggaaatcgaa atgcccatca ctcaaacaat ctatgaagtg ctgtatgaac aaaaagaaat
    25681 caaaacagcc gcaaaagaaa tcatgttacg tgatggaaaa aaagaaaatg aattctcatt
    25741 aacaattttt tgaaggagcg aatgtatatg aaagttaaaa aagcagttat tccagcagca
    25801 ggattaggaa caagattttt accagcaaca aaagctatgg caaaggaaat gttgccaatt
    25861 gttgataaac caacaatcca gttcatcgtt gaagaagcgt tgaaatcagg tatcgaagac
    25921 atcttgatcg ttacaggaaa agctaaacga ccaatcgaag atcactttga ttcaaatatt
    25981 gaattagaaa caaacctaag tgaaaaagga aagaccgaat tattgaaatt agtagaagaa
    26041 acgacagatg tcaacttgca tttcatccgt caatctcatc caaagggttt aggacatgcc
    26101 gttcttcaag caagagcctt tattggaaat gaaccatttg tcgttatgct aggcgatgat
    26161 ataatggaag acgaagtacc attgacacag caattaatcg atgattacga acgtactcat
    26221 gcttctacga tcgccgttat gcaagttcct catgatgaaa cttcaaaata cggaattatc
    26281 aatccaggag aagtattgga agatgggtta tacaatgtca aaaactttgt tgaaaaacca
    26341 gatccaagca aagcaccaag tgatttagct attattggac gttacttatt gactccagaa
    26401 atttttgatg tattgtccga gcaacaacca ggagccggaa atgaaatcca actaactgac
    26461 gcaatcgata cattgaataa aacacaacgc gtatttgccc gtaaattcac aggtaaacgt
    26521 tacgatgtcg gtgacaaatt tggttttatg aaaacaagta ttgaatatgg tctaacacat
    26581 ccagaagtag gtgcaccttt acgtgagtac atcctgtctt taggtaaaac attagaagca
    26641 gaaggaagca agatcgatca taatccaaaa ggaataacag aaatcaaaga agaaaagaaa
    26701 aaataaccct tttttctaac gagcttcagc tgctaattga ataaaaagta gttgaagctc
    26761 gtttttattt gtttaaaaaa cacgaaaatg ctatccttat agaaaagaga gacaagaagg
    26821 gagtttgatc atgtcaggag gagaaattgc aggattgatc gcagcaatcg cctttgcgat
    26881 acttgtcgtt tttatcgttc gtgtgttgtt gcaagtttct aaaactttag aaaaagtaga
    26941 tcagactgta gcagaagcaa atacgacgat cgaaatcgtc acaaaagacg tagatctttt
    27001 gtctagacaa gtagaaggct tactggtcaa aagtaatgaa ctgctcttgg atatcaatgg
    27061 aaaagtcgca acgatcgatc cattgttcac agcagttgct gatttaagtg aaagtgtatc
    27121 agaacttaat cattctagta aaaacatcgc gacgaaagtc ggcggaatcg gtaaaacgac
    27181 agctaaagcg acggttgccg gaaaagtcgg tgggacagcg atgaaatttt ttaaaaacaa
    27241 aaaacatact gaaacgacag gaggaaaata aaaatggcta aaaaaggtgg atttttatta
    27301 ggtgcagtag tcggaggcac agccgcagca atcgccgcgt tattgttagc accaaaatct
    27361 ggtaaagaac ttcgggatga attagctgcc caagcggatg atttgatgga tatggcttca
    27421 gattacacag atatggcgaa agaaaaatcg actgagttaa caggtattgc aaaagaaaaa
    27481 gcgcaagatc tttctcaaca agcaggtgat ctagcaggta gtgtcaaaca aaaagtaagt
    27541 gaatcagcag atgaagcagg aagtgaatct gacgatatct tgttaaatct taaagaacaa
    27601 tcagctgact tatctaatcg tttcaaaaaa acggcagaag atatgaaaaa acctgcagat
    27661 gaattaggaa gtattgctaa agatacttca gaagatatca tgattgatgt aaaagacgct
    27721 gctggtaaag caggagatac tgtcaatgaa ggaatgtcag aagcaaaacc agttgcagat
    27781 gactcaaaag aaatgatgaa aatggcaaaa gaagatgcga aagaagctaa aaaagaaata
    27841 aaagatacaa tgacagattc atctacaaat cactaaaatc acaaatttat agaataaaaa
    27901 ggtgggagcg gactagctct cacctttttt actcttattt ttctaaaaca agtaattctt
    27961 ttggtgtagt cgtgaatggc acacagccat cgcttgtcac atatacacag tcttcgatac
    28021 gtacccctac tttttcagga atatagattc ctggttcaag agagaagcac atcccttctt
    28081 cgattactaa gtcatttcct tctacaaggg aagggaattc gtggacggtt gttccgatcc
    28141 cgtgacctaa acggtgattg aaatattcgc cgtagccata gctgttgatc acgtttcgtg
    28201 cgatttgatc aagttcacct gctgtcacac ctggacggac ggcttcttgt gcggcaagct
    28261 gtgcttcaag agtgatgttg tagatttttt cttggaagtc ggttggtttt tgataagcaa
    28321 ttgtacgagt agcatcactg caatagccat tccaaacaac gccaagatcg aataacacaa
    28381 gttcattcgg tgaaatcttc gtatttcctg gtgtaccatg agggcttgca gcatttgctc
    28441 ctgcaaggac taatgtgtcg aaagacattt gtgaaacacc ttgttttttc aattggtatt
    28501 caatttctgc aatgatcgct tgttcagtca cgccttcttt cactgcttta aagccaatct
    28561 caaatgcaac atcggcccat ttaccagctt cgatcagttt gtctttttcc tctagagttt
    28621 tgatcagttg tagacgttgg atgatggaag ttagatcagt gtaagaatcc gttcccgtca
    28681 agatatcaga gatagcatcg aagcgctcga cagtcaaggc gttcttttca atccctacat
    28741 tgccaggctg accatagcga gcacgaagtt cttttccgat gatctcccaa gggttttcgc
    28801 tgtctaaata gccacggaca tcatacgccc aactactgtt ttgagcatct tcaacttcca
    28861 aagcaggagt gaaaaggaaa ggatcttgtt caagagggat aaaaagagct agtacacgct
    28921 catgaggatc actttcgtat ccggaaaagt aagcgatgtg gccagggtcg ctgatataag
    28981 ccaattcgac ttggtgttgt gccatccatt gacgtagttc attgattttt acgttattca
    29041 tttggcaacc tactttctaa attaattttg tattcaataa ttgtaccact gatttattaa
    29101 aagaccaacg ttctaaaaac tgaaaaaagt aaaaattttc atgaaaacag agataaaacg
    29161 gttgcaattc ttgagattat ttgatattct attttagaaa tgtaagacgt attttcagaa
    29221 aacatataaa atatgaatag gagaacacaa atggaaaagc aaacaatcac tatctatgat
    29281 gtagcgagag aggcaaatgt ttcgatggct actgtttcac gtgtcgtaaa tggcaatcca
    29341 aacgttaaac cagcaacaag aaaaaaagtc ttggaagtta tcgatcgctt ggattatcgt
    29401 ccaaacgcag ttgcgcgtgg cttggcaagt aagaaaacaa caacagttgg tgtaatcatc
    29461 cctgatgtca gcaatatgtt ctttgcttct ttagcacgtg gtatcgatga tgtggcaaca
    29521 atgtacaaat acaatattat cttagcaaat tcagatggca acgaccaaaa agaagtcaat
    29581 gtattgaata acttactggc taagcaagtg gacggcgtga tcttcatggg tcaccatatc
    29641 acagacgaaa tccgtggaga attttcacgt tcaaaaactc ctgttgtatt agctggctct
    29701 attgatccag atgaacaagt aggtagcgta aacattgatt atgtatccgc aacaaaagat
    29761 gcagtaaaca aattggcaaa aagcggcaat aaaaaaatcg cttttgtcag tggtgcgttg
    29821 atcgatccca tcaatggaca aaatcgtctg aaaggctata aagaagcatt aagcgaaaat
    29881 aacctaccat atagcgaagg attgatcttt gaagcacaat acaacttcaa agatggtcta
    29941 tctctagtcg atcggatcca taatagcggt gcaacagctg tctatgtttc agatgatgaa
    30001 ttagccatcg gtattttgga tggtttatta gaccgtggcg tgaaagtacc ggaagaattc
    30061 gaaatcatca caagtaacaa ttcactactg acagaagtag ctcgtcctcg tttaacaagt
    30121 atcacacaac cattatacga tttaggggcg gtatctatgc gcctattgac gaaaatgatg
    30181 aataaagaag aagtcgaaga aaaaacgatt atcttgcctt atggaatcga agaaaaagga
    30241 tctacaaaat aataaaagga caaccattca gaggttgtgt gcctgccgtt taactctgcg
    30301 ttacaaggag caatttcaaa aaacagcatc tcatgtttga tgaatattgt gactcgtgat
    30361 tcggagttgg caggagaaca ccgtttattt gggaaaaggg gttatgacaa aattttgtta
    30421 taatcctttt tttgtgtctt aaaaaagaga gaaagtggtt gaaattacat ttatcgtata
    30481 taaaacaatc cgtgtataga tgctgcattt tgttcaccac catcaaaaaa ataagggact
    30541 gtgacaatac tattgtcaca atcccttatt actgtgtatt ctattaaatc aatcatcttt
    30601 ctcatctgat tctttagttg cttgattcgc tcttgcataa gctgaagcgg tgttccagta
    30661 atctgtatag ttttcatcgg taccgccaac gccaaattta aaggcgcttt tcttcggtcc
    30721 atttttcgcc catagcgatt caactgtttt tcctggtgtt tggatggatt ttttattgac
    30781 agtaactttt ccaggtgttt gaccagtaaa tccagatact tttttcttca tcacatcaga
    30841 tgataattcg aatttctcat cagtgccaaa aatgtcaggt tttgcttggt aagcttgatt
    30901 gatcagattg gcaagataat tagccgaacg tatccttgct ttgctgtcca ttccagtatt
    30961 gtcatcgtgc cctgcccagc tactgatggt aatagacgga gtagagacaa ttagccatga
    31021 atctcggtat tcattggtag aaccagtttt tccgacccaa tctgcttttc ctagatttcc
    31081 gtttaagctt gagatggcat ctttgaatgg tgtagtgatt tttgcattga tcacactacg
    31141 catcatgtca ttcatgatcg atgccgttgc ttccgaatag atccggacag agttgctttc
    31201 gtgtttataa attacatttc ctgcgttatc tgtgatggaa tcgatgatat agccttcttc
    31261 atagacgccg ccgtttgcta aagcctgaaa tccgttggtc tgttgaagcg tcgtgacatc
    31321 tactgtacct aaaggagcag attcaacgcc ccaattgtca tttgccgggt agttcatctt
    31381 tgccaagtag tgctcgtaag catattgttt cgatccaccg gaattcaaca catcttgata
    31441 taagtgataa gccgggatat tgttggacca ttctagtgac tcacggacgg tttggaaggt
    31501 gttcgatcct tcgttagtag cattgacgat tttctcgcca gcattttctc cttcttgcca
    31561 agttgttgca taatctgata cacgggattc tgatccgata agccccatgt cgattgcagg
    31621 tccataaaca agaactggtt tgattgatga gccagcttga cgttctgtat cgaaggcatg
    31681 gttattttga ttctgactgt aattccgtcc gccaacgaat ccgtagatgc ggccagttct
    31741 gttatccatc aatacatttc cggtttctac ttgactgcca gcaccatcat ctaacatgta
    31801 tccataattt tcaacgccat tttgcaatgc agcatagaca tctttatcga ttgtcgaatg
    31861 gattgtatat cctttatttt ggatcgtttg ctgtgccttt tgataataag cgtcactgac
    31921 cgatgaatca gacatatcag cgttgtcttt ttcagccaat tgttgtgcaa tgattcttgt
    31981 tgcttcattc atcacagtat aatacaagaa ttctctgtca gtctgttctg cgatttgttg
    32041 tggtagaaag tcttttgtaa ggtcataggc ttttgcttct tcgtattgtt ctttcgtaat
    32101 ttggccttca cgatacatac tgaataaaac aaaatctttt ctggcaaggc cggcagaaag
    32161 atcttctttt agcgcgcctg tgttcgtgta tggactatac gtgatcgggc tttggggtaa
    32221 tccagcgata taagcagctt gcggcagcgt cacatctttt gcatctacac caaagattcc
    32281 ttttgcagct tcctggactc cagcaatgtt ttgtccctta ttattacgac caaatggtga
    32341 gacattcaaa tacgtagcaa tgatctcatc ttttgaaaag tatttttcta cttgtgcaga
    32401 aagaatgatc tcgtttgctt tacgtttgaa tgttgtttca tctgtaagga tctgctgttt
    32461 gactaattgc tgtgtcaaag ttgatccacc agaagagcca ataccagtag cttcagatac
    32521 taatgctcgt aaaacagctt taggaacgta accttggtgt ttatcgaagt attcatcctc
    32581 tgtgctgata attgcagttt tcaataaagg tgaaatctta tcagatgaaa tagtcgtgcg
    32641 catcagatct gtctggattt ttgatatctc tgtattatct gcataaacaa tctttgacgt
    32701 ttctgtaatg tttcctagtt ctgtctgcag tgctttttta gtcggcaatt gtgtgtcttc
    32761 gacgagatag gcaaaatagc cagcgcctat tccaatacct agagctgcga acatcaaaag
    32821 aagaacagta ataaaaacag tcaatgattg aaaaactcta aaaataatct taggaacaaa
    32881 ccatttttct ttttttcttc tattctgttt ttttcgagta tttttgcgtg gcaaaagggt
    32941 cacctcaact aatggtttta tttcgctaat tatagcaaat aatagaacaa aaaaacagac
    33001 aaggctgttt ttggcaggaa tttctttttt aaaagagtta aaacgtgaga aaatgatctt
    33061 tattataaaa gagttcttaa gcttttcttt agagaaaatt tcagttacta tggagaacag
    33121 ggcaaagaaa aatcaatcat tttctttgta tatcatctta ccaatatata ggaattgtta
    33181 gtaacaattg cctttcaagg aggaatccat ccatgttatt aggctcacac gtcagcatga
    33241 gcggaaagaa aatgctgtta ggagcagcag aagaagcagc cagttatgga tcttcgactt
    33301 ttatgatcta tacaggcgcc cctcaaaaca ctagaagaaa agccatcgaa gagatgaata
    33361 ttgatgccgg aaaagcgtac atggcagaaa aaggtctaac aaatatcgtc gttcatgccc
    33421 cttatatcat taatttgggg aatacgatca aaccagaaat gtttcctttt gccgtccaat
    33481 ttctgagaga agagattgtt cgtgcggaag ctttaggcgc tactcaaatt acacttcacc
    33541 caggtgctca tgtaggggca ggaacagaag ccggaattgc tagaattatt gaaggcttga
    33601 atgaagtttt gacaaaagaa cagacggcac aaatcgcttt ggagacaatg gcaggtaaag
    33661 gtacggaaat cggtcgaaca tttgaggaat tagcgcaaat catcgatgga gtaaaattga
    33721 atgaaaaatt atccgttact tttgacacct gtcatacgaa tgatgccggt tataatatta
    33781 aagaagactt ttcaggtgta atggaagagt ttgataagat catcgggtta gatcggctta
    33841 aagtactcca tatcaatgat tctaaaaatc cgcaaggttc gcataaagac cggcacgcca
    33901 atattggttt tggaacaatc ggattcgaca cgctgaataa gatcgtccat gatccaatgt
    33961 ttgacaatat ttcgaaaatc ttggaaactc cctatgtagg caaagataaa aagaaagctt
    34021 acgcaccata tggaaaagaa atcgccatgt tcaaagcgca aacattcgat ccagaagtgt
    34081 ttcctgagtt attagcagaa gaagtgatac tctaagattt tctatctgac agcagcaaaa
    34141 aaggaccagg cacattgcct tgtccttttt tgtttaagaa acaaaggagt actttttgca
    34201 gtctttttca tcatgcatct tctcccccct ctttttaaat ttagacaaac aaggtatact
    34261 ggtagaaaag taaaaaaacg aaaagtttca caagcaagtg gcaaaacgtg attggaagga
    34321 cgaaaagatg ctgaacactt taaaaccctt gatcaaccaa ctaaaaaaga aacatacata
    34381 ctttattctt ggcatagccc tattatttac aattattcgt ttgtatttat atagtaaagc
    34441 aatctatgga acagacatgt cggcaaccta tgatgaccag ctgctgattc gctatgctca
    34501 aaatattttg gacggtaaat ggctagggga gtatacgact acaacacttt ccaaaggaat
    34561 ctcctacagc ttatttttag tacttgcaca taaactgcat ctttcttatg cagttttgtt
    34621 cggcatgttc agtatttttg ccagtatatt ggttgcactc gcgtttaaac cgattgtgaa
    34681 gaacaagtat cttcttttaa gtatctattt ttattttttg ttttctccga ttagttttac
    34741 acatgaatat tccactcgaa catatcgtaa tgccctcgtg attccggctg ttgagatcat
    34801 tatcgcttgt ttattagcta tttattaccg caaatacggt tctagcagaa aattgctgcc
    34861 atggttttta gggctttctg tcgcttttcc tttcttttgg ttcattcgcg aagattctat
    34921 ctggcttctt ccttttactg tagtagcttt gggtatcagt ttcgtccaga tcttgctagg
    34981 gcaagacttt tctttcaaag aagtaaagaa gttaaaaaaa cgactctttc ttctctcgaa
    35041 acgggaatgg ctgaaacttt cattgttttt acttcctata gttggctttt ccatcgtcta
    35101 tcaatggatc gaatataaaa atgagacaac atacggtatt gcaaccatca atgaccgttc
    35161 cagtggagaa tttggacgtt tgatgaaaca actgatccga atagatgatg aaacgaactt
    35221 gaataaagaa aacagtgaga tatgggtctc acatgctgca ctaaataaag cattagccgc
    35281 atctcctaca ttccatactg tttctacccg tattgacgaa ttatatacga ctcacgcgtg
    35341 gactcaaggt ggaaaagtaa aagaattacc cggagatatc atcttttggg cattgcgaga
    35401 tgtggtaagc gaatctggtt attaccaaaa caatgcaaaa gaaaccaatg atttttggaa
    35461 aaaagtcaac caagaattag aagaagccta tcacaaggga acaatcaaaa agaaaaaaga
    35521 gttctatctg atgggatcag gagatggaaa gacgatgagt gatctgccaa tcgtattcga
    35581 tttccttcaa tcaggatacg cttatgcatg gcgatataaa gatttccatc aaggaggaaa
    35641 ttatagtttc ggaacgcaag aacaagtcaa acaggctcaa gaattattaa accttccgct
    35701 aatggagaat tggatcgata gtaatgaacc gagaacacct aatttccaat taacaaaatc
    35761 agcaaaagtc gcgaatgtca tcataaaaat ttaccaagtt ctggctattc ccgtgctgat
    35821 tgcttcaata gcagggatat tacttatgat actaggaacc gtatttgcaa aagctgatcg
    35881 tgcctattat agttcctctt tgattgtggt caccggatta atactgacac aattggtttt
    35941 tcttttcggt gtctcatggt tttgttctta tgcgccagaa aaaaaggact attttttaag
    36001 tgtctacact ggtgcaggcg tgccattaac gcaattcatc gtagtaacaa gcatgctagt
    36061 tttcagtaaa ttaaatctga aaaaagcttt atcttctaaa taaaaagacc cttggtagct
    36121 agatttttaa cctaactatc aagggtcttt gtgtggaaaa atcagttttt attcgatttc
    36181 ttctaccgtt tcctgcaaaa gctgttcata ttttttgttt cctttatata aatcaccaat
    36241 cgtccatgcc aacaggagaa ggacaagaac aatgattaca taagcgatgt gatcagccaa
    36301 tgctagttga gcggaagtgg cttgctcgcc gaagaaactt tcgatctgtc ctggtaatcc
    36361 tcgtaagttc aagtaagtta gactaatcac agagaaccag ccaagaagtt ttacgatgaa
    36421 tgaattttta aatcggctac ccatctccgc ttggctgttg gtcatcatca gtaaaggaat
    36481 catcgaaaac ggcaacgcaa aagccaagaa cacctgggat tcattcatta agttattcaa
    36541 agcggcatgt tcttcgatcg ttccttttgt acttgtaaac aaaacacaaa tcaatacagg
    36601 aatcacagaa agcaatcgtg tgaccagtct tcttagccaa atcgggatac gcatatggat
    36661 gaatccttcc atgatgactt gaccggtcaa cgtaccagta atagtagagt tctggccaga
    36721 agcaagaagt gctacagcaa ataaagtgga caagatacca gattttgcta cagcaatcaa
    36781 aataccattg ctcatagctg atggatcaga taaagcttca tacagaccaa aaaaggaagg
    36841 atcttggaca gaaccggttt tgaagacagc gacacccatg atcaatagca gtgcattgac
    36901 tagaaaagcc attgacaact ggatattaga atcccaagta gaaaaacgta cagcttgtgc
    36961 tacatcttct tcgttttggt gatcgatctt ccgagtttgt gaaactgctg aatgaagata
    37021 taagttatgc ggcatgactg ttgcaccaat aatccctaaa gctccagtca gcggagtttc
    37081 gccgccgatg accggtgagt ctgagaatgt atgactgtta gggataaagc cagcaaataa
    37141 actgtgccaa tctgggttag acaagccaac ttgatagaca aaaacgaata aaatgacgaa
    37201 aattaagcag acaaccagtg cttcgatctt acgaaacccg acttttgtca gtagtaagag
    37261 aacgaaaaca tctaatacag taataaagac ggcaatcacc aatggaatat caaaaagtaa
    37321 gtagagggcg attgctgcac caatgacttc tgcaatatct gttgccataa tggctaattc
    37381 agtcaagatc cataaaatga cacctaatgt cttacttgtg cgtgctcgga ttgcctgtgc
    37441 caaatccatt tggctcacga ttcctaattt tgctgccata tactgtaaaa gcatagcaat
    37501 cagactagac atcaaaataa ctgacatcaa taaatattgg aaattttgtc ctccggtgat
    37561 cgaagtagac cagttacccg ggtccatata cccaactgct actaacgcac caggtccaga
    37621 gtaagcaaga agtgttttaa aaaaactttt cccctttggt acatcaactg ttccatttac
    37681 ttcagctaaa gataaaccat ttgtatgttc aataagtcga tgcttatgaa attgtttatt
    37741 ggattgatta ttcaagaaaa aaccaccttt cctatttatc tacgattaca ttctacacct
    37801 caaaaagaaa aaattaaaga aaaagattcg gtacgcctaa ttttttttca tggcgcgtct
    37861 tgctagatga cgaaagaaat tttgttctcc tctaaaaaaa ccactatttt ttgcatgaaa
    37921 ctaaagaata cgcctttctt ttttcaataa actttagtaa aataagtcat tgagagcaat
    37981 tcggaaatag aaaggagaga tttatatgtt atcaacaggt attgtcgtac ttattgctat
    38041 tattgctttg ctaatcggcg cagtaggtgg ctttttccta gcgcgtaaat atatgaaaga
    38101 ctatttagaa aaaaatcctc ccgttaatga ggacatgttg cgatcaatga tgatgtcaat
    38161 ggggcaaaaa ccatctgaga agaaaattcg ccagatgatg cagcaaatga agaaccaagg
    38221 gaaaaaatag gttctgaccc aacgactttt tacgggctga ttttttcagt ccgtttttta
    38281 tttcgtaatt tgttagacta ttcagattat tcagcaaaaa agacaagcac agacaatcct
    38341 aggctcttga taaagacaaa ggaggagtaa aatgtcgatt tttagaaaat taggctggtt
    38401 ttttcgtcag gaaaagaaac attatttaat tggggttttc tcgctattcg cagtggcctt
    38461 ggtacagtta gttcctccaa aagtgattgg gatcatcatt gatgagatcg ccgataaaaa
    38521 tttatcaata aagatcattt tcgggtggat cttcctattg attatcgctg cttttttaca
    38581 atatcttttt cgttatatct ggcggacgaa tatttgggga agtgcagctc gattggagaa
    38641 agaacttcgg cagcagttat tcaaacattt tacagaaatg gacaatctct tttaccaaaa
    38701 atatcggact ggggatttga tggctcacgc aacgaatgat ttgaatgcga tccagaatgt
    38761 agcaggagct ggtatcttga cctttgcaga ttcagtgatt acgggcggaa caacgatcat
    38821 tgcgatgatt ttgtttattg attggcgctt aacactgatt gcgctgattc ctctgccttt
    38881 gctggccgtc acttcacgag tgctggggtc aaaactccat gatgcttttc gtgattcgca
    38941 agaagctttt tctagtatca atgataagac acaagaaagt atcactggga tcaaagtcat
    39001 taaaacattt ggacaagaaa aagaagatct agcggatttt gaagaaaaga ttgaagatgc
    39061 aattattaaa aacaaacgag tcaatttttt agactcctta tttgatccat tcattacctt
    39121 gattatcggt ctgtcttatg ttgcaacaat tattttaggt ggaaagtatg tcttggaagg
    39181 cacgatctcg atcgggcaga tggtctcatt tattagttat atcggtatgc ttgtttggcc
    39241 gatgtttgcg attggtcgat tgttcaacgt attggaaaga ggaaatgctt cttatgatcg
    39301 cgtcaatgaa cttttgcatg aaaagacgca tatcgtggaa caactgggtg cagtgcagac
    39361 cccggcaaaa ggcgagctga atgtagctat ttcctctttt aattatccga aagaagaatc
    39421 accagcatta gaacaaatca agtttgcgat ccaagaagga gaaacattag gtattgttgg
    39481 gaaaactggt gcgggaaaaa cgacgatcat caaattactg atgcgtgaat acgatgatta
    39541 tcaagggaaa atccaatttg gcggacgttc gatcaaagaa tattcattga atgctttatt
    39601 aggatcaatc ggctatgtgc ctcaggatca cttcttgttt tcgatgacca ttcgagataa
    39661 tatccgtttt tctaatccaa aactttcaga agaagaagta gagcatgcgg cttctttagc
    39721 atttatcaac aatgaaatca aggaattacc aaaaggttat gataccttag ttggagaacg
    39781 tggagtctct ttatctggcg gacagaaaca aaggatttcg attgctcgag cattgatcaa
    39841 aaatcctgaa ttattaatat tagatgatgc actctctgca gtagatgcca aaacagaaga
    39901 agctatcttg cataatttaa aagaaataag aaaagagaaa acaactatca ttgcagctca
    39961 ccgtttaagt agcgtgatgc atgcaaaaga aatcattgtt gtcgatcaag ggaaaatcat
    40021 tgaacgagga acccatcatg agctgcttgc actaggcggc tggtatgaac aaatgtggga
    40081 gaaacagcaa ttagaagcga agatagaagg aggcgaggcc tgatggatga aaattatcaa
    40141 tcggaatgga caaaatcgat tccacttaaa gaacagaaaa agatcatcaa acgactgatg
    40201 aagttcgcca aaccttttag aagaacgttc atcgttgcta tcttatttgc gtttgctttg
    40261 tctgtgatca atgtcctatt gccaaggatc atccaaactt ttatggatga tcatctggct
    40321 aaacagtcgg ctacgacaca agttattttg ttttttgctg gtatgtactt atttggagtc
    40381 atagtaaaaa gtatcatttg gtttttccaa tggtatttgt attccatggc ctcattgaag
    40441 acctatcagt atattagggt aaaattattt gaaaagctgc atacactagg tatgcgttat
    40501 tttgatcaga caccggcagg atcaaccgtt tctcgagtga cgaatgacac agaaacgcta
    40561 tttgaatttt ggtacgtctt tttgatggta ttgactggga tctttgcagt cgtttcttca
    40621 ttccttgcca tgtttcaaat caatggagaa attgccttgt ggactttggt gtttttgccg
    40681 attttaggaa tcgtcatctg gtattaccag aaattcagtt ctcgtattta ccgaaatatg
    40741 cgggaaaaat taagtcagct gaacacgaaa ttgaatgaat cgatttccgg catgcgtatt
    40801 atccagcagt ttcgccaaga agctcgttta gctaaagaat ttgaacaagt gaacaatgag
    40861 tatttagaca cgcgttatgc gatgatcaaa acgaattcga ttctgttaag tccgatcatc
    40921 aattttctat atgcattggc gattgcgttg gtgttgactt tgtttgggat cgatgcgttg
    40981 cattctcccg tagaagttgg actgatctat gcatttacga cctatgtcca gggatttttt
    41041 aatccaatgt ctcaaatgat ggactttttg agcatcttca cagatgggat ggttgctggt
    41101 agtcgtatct tgaagattat ggatacggaa gaatatgcac cacaacaaaa ccctggagca
    41161 gatcaaatga ttacttcagg taagattgag tttcgcaatg taagcttttc ttatgatgga
    41221 gagcacaatg tattaaacaa tatctctttt gttgctaatc cgggagagac cgttgcatta
    41281 gtgggacaca ccggaagcgg aaaaagctcg atcatcaacg tattgatgcg gttttacgaa
    41341 ttttatgaag gtcagatatt gattgatgat catgacattc gagaatatcc aatggaagag
    41401 ttacgtaaga aaatggggct ggtcttgcaa gatgctttct tgttttatgg ggatatcgca
    41461 ggaaatattc ggatgatgaa tccagctatc acagacgaac agatacgcca tgcggctgag
    41521 tttgttcaag caaacaaatt catcgatgaa ttaccaaaag gatatcactc aaaagtgatc
    41581 gaacatggtg ccagctattc tagtggacaa cgacagctga tcacttttgc gcgaacaatc
    41641 gtgactgatc ctaaaattct agtattggat gaagcaacag caaatatcga tactgaaaca
    41701 gaaggattga tccaagaagg attggcaaaa atgcgtcaag gacggacaac gattgcgatt
    41761 gcccatcggc tgtcaacgat tcgagatgca gaattgattt tagttttaga caaaggtcat
    41821 atagtagaac gcgggaatca tgaaacactt ctagcgcaaa atggtttgta tgcagatatg
    41881 tacaaactgc aaagcagtgg tatataaaag atgttgtgac agtagaattt tgacctaagc
    41941 actagagtga aaataaaaca tagcttctca tatttttcat ctgtgagagt tagtgtcgta
    42001 gtcaccagat ttaaaaaaag attcgacagc tagtcagaag tgcctaatag ccactaagaa
    42061 agcgttgaca aaagcatttg ctttcgataa tataaagaaa acgatacaac ttggatagtg
    42121 attgcctgat gcaagctaaa cctgatgagt ctcccatggt ttcatggacg gctttttggg
    42181 tttttctttt tgttttggat acagccctat acatcatgga aaggagcaaa acatgctgat
    42241 tgagaaagta ctgaataaca acgtcgtaat aaccagggat gaaagaaatc aggaaatgat
    42301 cgtgatggga aaaggtttgg cattcaaaaa gaaagtgggt gacccgattt ttgactcatg
    42361 catcgacaaa aaatttcagt tgacaaatga cgggctttca caacagttcc aagaattggc
    42421 acaagcgatt cctcttgagt acttagaaat ttcttgtgaa atcattgaat atacaaaaga
    42481 ggctctacaa gtccagttga atgattctgt gtatattgca ttgacggatc atttgtatac
    42541 ggctatcgaa cgtcacaaaa tgggaatatt tgtaccaaat gtattgctgt atgatatcaa
    42601 acaactattt cctaaagagt atgccatcgg gaaaaaaaca gtgaagaaaa tctcggataa
    42661 ataccacatc aagttatcag aagatgaagc cggatttatc gcattgcatt tagtcaatgc
    42721 acaaggcatt caaagccagc aagacaatac ctacctgttt actataacaa tacaggatat
    42781 tctaaatatc gtccgctatt actataagat tacatttgat gaagattcag tccatttttt
    42841 tcgattcgtc acacacttac gtttttttct gacacgcgtc ctagatggag aaaaatcaga
    42901 agtgggagaa gtttctgagg aattgtttca ggttattcaa aaaaaatata aggatacgtt
    42961 tggttgcgtt gagaaaatag cagacttttt agccgagacg ttcaactacc agatgtccac
    43021 ggacgaaaaa atgtatttaa cgatccatac tgcgcgttta gtgcatgaga atcgttgtaa
    43081 ataaaaaaag gaggataaat taacaagaac gtatgaaata agggtagtga cattcagttg
    43141 gctaaacctt ccactaataa attggaggga atagaaatgg gaaaatacca agaattagcg
    43201 agaaaaatcg tagaaaatgt cggcgggaag gaaaatatca atggtttgac acattgtatc
    43261 acaaggcttc gtttcaaatt aaaaaacgaa gaaaaagcaa atgatgagat cttgaaaaat
    43321 atggacggta ttgtaacggt aatgcgagcg ggagggcagt atcaagttgt cattgggaat
    43381 catgtgccag tcgtgtttga agaggttata aaagcaggaa accttacttt tgatgaggct
    43441 gtttcaacag aaaaaatgag accgtttgat atgcttattg atggaatatc gggctgtttc
    43501 cagccattcc ttgctatcct agctgctggc gggatgattc gtggactgac agcattcctt
    43561 gtcttcttag gcgcgtttga ccgcggttct ggcacatttg ttatgtttga taatatcggt
    43621 gattctgtat tccaatttat gcctgttatt atcggtttaa cagctgcccg taaatttaaa
    43681 gtaaatgaat tcgtagggat gttgcttggc gcagcattaa tgaatcctag cttgtcgctg
    43741 gaagctttga gtggtgcagc agaagcacca ctaacgacca tattttctgg cactattttt
    43801 gaagcaccta tttatcaaac agtattcggc attccctgga ttgcgcgtaa ctacgcttct
    43861 tctgtcattc ccattatctt tattgtgttg ttagctagcc aaattcagaa accaattaaa
    43921 aagttagtac ctgagatgat cgcgaatttc ttcgttccat tcttcaccgt tcttattaca
    43981 atgcctttag gttttttact cgttggacca gtattcacat tcgcaactga tattttaatg
    44041 gctggatttg aaacactttt agcactttcg ccagttattt atggagcaat cgtaggcttc
    44101 ttctggcaaa ttctggtgat gtttggtctc cattgggcaa tcgtaccaat gggattgatg
    44161 caattttctg tgaatggttg gcaaaatatt atgacacctg ttgcagtagt gtcttttgga
    44221 caaactgcag cacttaccgc actctacttt aagcttagaa atccaaaaga caaagcaatt
    44281 gcaataccag ccattgtttc tggtattgtg gggattacag agccagctat ttatgggttt
    44341 acattaccac gtaaaaaaat atttattttt acctgtgtcg gtggtgcgtt agggggcgcg
    44401 tatagtgggt taatgaattt aacttcttgg aatcaaggtg gactaggcat ctttacaatt
    44461 cccaactata ttagaccaga tggtgattta acagatgtga ttaatgtatt gattgggatc
    44521 gccattgcga tggtggtatc ctttacgctg acttttttct tttggaagga tgaagcaggt
    44581 gaaacagatg atatacagaa aaaatcaggt aaagaaattg taaaaacacc gatacaagga
    44641 caaatcgctc cactaaatgc agcaaaggat gcagcttttg cacaaggaac actaggtcga
    44701 ggaatcttga tctaccctga aaaaggagaa gtacgtgccc cttttgacgg aacagtcatg
    44761 accctgttcc cgacgaaaca ggcaattgga atggtttctg aaacagggct ggaattgctg
    44821 attcatgtcg gattggatac tgtgcagttg gaagggaaat attttgaaag cttagtgcag
    44881 caaggataaa gagtgaaaca aggcgatctg ttgctccgct tcgacaaaaa gaaaatcgaa
    44941 aaggaaggat acagtttaga aacacctgta atcgtcacca attacatgga ttatttggat
    45001 attttggaaa atcaagggga gacggtcggg ccagacgatg cacttatcac cgcactgacc
    45061 tgaacggtta ggtgaaagaa tggaatagac ttaactattc tttttagtcc acttctttat
    45121 tttcaaaatt gaaaggctct ttcatgatgg ctgagctgat agggcgttta tcaaattttt
    45181 gtcctacaga aattagatgg actaaattct gttgataatt ttctgcaaaa acacaactat
    45241 ataacacgtc tagaagataa caaatcgatg tattgatcgt aaaattacca atttttgaat
    45301 acagtcgttc gcgtgtcgta agtcgcaaag cggctgtact cattgaggat agcgttgaat
    45361 caccgatact ggtcaacgct aaaattggaa catgattttg tttcaatatt tctgcggtac
    45421 gaaggatcac attgttttca cccgtataag aaatcagaat cccgcacata tcactcgtgc
    45481 aattgtacgc ttcatagaac gattcaccaa aagtggtgac aatcgtgaca tccattttga
    45541 tgcgcttcat tttcagcatg aaatcttgag aaatcaaagt attcgagtta ctggcgaaaa
    45601 tcacgatttt ttttgcttga agcattagtt gtttggcttt ttgcagttgg tcatgatgaa
    45661 tgagagaaag tgtatcttga atcgtcatct gttctaaaga agccatttta tttgcgatcg
    45721 tcataatacc gtctgtggtg gaaaaaggaa gattcgcatc tacgtcttga aaattccctt
    45781 caagataggc tgtttcagct aaaaaagcat tacgaaaact gacccagcca tcaaaaccca
    45841 ttttttttgc tacacggatg agtgtggacg ggtggacata acatcgctca gcaatctctt
    45901 ggatggtcat ttcgccaagt gctggagaat cgcttaagag aaactgaatc acttcatttt
    45961 ctgaaggaga aaaggagatt tctttgattt tttcacgtaa taacatgtag ataccttctt
    46021 ctagtttttt tacgtaaatt ataacttttt tgaaaatgat ttgtaaacgg atttatgtta
    46081 tatagcttaa taaagtcatt ttttgacaag atatttacaa aaatccagta gaaaacacta
    46141 acttttgtaa atgttactgg aaagaaacga atttctattg tttgttttgt ttacaaaaaa
    46201 gataataaaa gtgtaaaaag caaaggagtg agaacatggc aaaaggaatc aaaatcgcta
    46261 cgattggtgg aggcagcagt tacacccccg agttgatgga aggatttatc aaaagatatg
    46321 aggaattacc gatacgagaa atctggctcg tagatgtaga agaaggaaaa gaaaagcttg
    46381 agatcgtcag tcagatgtct cagcgtatgt gggatgcttc tccgtatgaa gtcaagatct
    46441 atgcaacttt ggatcgggaa gaagcattga aagatgcgga ttttgttacg actcagtttc
    46501 gtgtcggtca attagatgca cgaatcaaag acgagcggat ccctagttat tatgggatga
    46561 ttgggcaaga aacaaatggt gctggcggga tattcaaagc ttttcgtacc gttccagtgg
    46621 ttttggcaat cgttgaagac atgaagcgtt tatgtccaaa tgcttggctg atcaatttcg
    46681 cgaacccaag cgggatggtg acagaagcag ttatcagata tggcaaatgg gataaagtga
    46741 tcggattatg caatgtgcca gtaatggcga cgatgacaga accagcaatg cttggcaaga
    46801 aaaaagaaga gctgatctac aagtttgccg gtctaaatca cttccattgg cacaaagtag
    46861 cagataataa aggaaatgac cgcacagcag atttgattga tcgtttatac gatgaagaca
    46921 atggattgcc gaaaaatatc tttgacatcc cgtttttcaa agaacaatta aaacaaatga
    46981 acatgatccc ttgtggctac caccgctatt actaccgtga agaagaaatg ttggaacatg
    47041 ctttagaaga atatcaaacg attggtacaa gagcgcaaca agtgaaagaa acagaagcag
    47101 agctgtttga attatacaaa gatccagaat tggatcataa acctgaacaa ttgcaaaaac
    47161 gaggtggtgc ttattattca gatgctgcct gtgaaacgat tgcttcgatc tatggaaata
    47221 aagaaacaca gatcgtggtg tctacaaaga atgatggagc tgttcctgat ctgccggcag
    47281 attgtgtagt agaagtcact gcctatgttg gcgcacaagg cgcacgaaat gtagcgtttg
    47341 gttcccttcc tgctgctgaa agaggctggt tgcaagtaat gaagaatatg gaattgctga
    47401 cgatcgaagc agccgtgact ggtgattatg gaacagcttt acaagcgttt acgatcaatc
    47461 cgcttatacg atcaggaaag acagcaaaac ggatcatgga tgagttgttt attgcacatc
    47521 agcagtattt accgcaattc aaggaaacaa tcagccggtt aaaagcagaa ggaatcgaag
    47581 tccaagatga actggcaaga gaattgtcaa gcaatacaca tgtaatggag gaagtatgat
    47641 gggattcaga aaagattttt tatggggcgg tgctacagca gccaatcaat gcgagggtgg
    47701 ttatgatgaa ggcggacgtg ggttagcgaa cgtagatgtt gtgccaatcg gagaagaccg
    47761 ttttcctgtc attactggaa aaaagaaaat gtttgaattt gataaagatc atttttatcc
    47821 agcaaaaatt gcgatcgata tgtatcatca ttataaggaa gatattgctt tgtttgcaga
    47881 aatggggttc aagacttatc ggatgtccat tgcttggagt cgtatttttc caatgggcga
    47941 tgaaaatgag ccaaatgaat ctggtttgaa attttatgag gatgtcttta aagaatgtca
    48001 aaagtacggg atcgagccgc ttgtgacgat tacacatttt gattgtccga tgcatttgat
    48061 caaagaatat ggtgcgtggc gttctagaga actcgttggt ttttatgaaa atctgtgccg
    48121 agtcatcttc aatcgctata aaggacttgt caaatattgg ttgacattca atgagatcaa
    48181 tatgatcctt catgcaccat tcatgggcgc agggctgtat tttgaagaag gagaaaatga
    48241 agaacaaatc aaatatcaag cggcacatca tgagttggtt gcaagtgcca tcgcaacgaa
    48301 gattgctcat gaagtagatc cggagaacaa agtaggctgt atgctggcag caggagcata
    48361 ttatccatac acttgcaatc ctgaagatgt ttgggcttct agaaaatcag atcgagaaaa
    48421 ttatttcttc attgatgtcc agtcacgagg agaatatccc gcttatgcat tgaaagaaat
    48481 ggaacgaaat ggcattcaga tcaaaatgga acctggggat gccgcattgc tgaaagaaca
    48541 tactgttgat tttatctcct tttcttatta ttcttctcga gtttcgacta ctgacaacga
    48601 actcttggaa cagactgcag gaaatatctt tgcttctgtc aaaaatccat atctggaagc
    48661 aagtgaatgg ggttggcaaa ttgatccatt aggcttgaga ataacgatga atgatattta
    48721 tgatcgttat caaaaaccac tatttattgt agaaaacggc ttaggtgcag tcgatacgcc
    48781 cgatgagaat ggttatgtgg aagatgacta tcgaatcgac tatctggcag cacatatcca
    48841 ggcaatgaaa gatacagtag aattagatgg agtcgatcta ttaggctata cgacttgggg
    48901 atgtattgat ttagtatcag ctggtacagg tgagatgaag aaaaggtacg gattcattta
    48961 tgtcgatcgt gacaacgaag gaaatggcac attagcccgt tcgaagaaga aatcattcaa
    49021 ctggtataaa aaagtaattg attcaaatgg agaaaatctt tcgaatgatt gatcaatgat
    49081 ttgtaaaagt ctattgtttt tcagcggatt tttcgagtat aataaagcta ttcgagaaac
    49141 gcaagcagaa caatagactt tttttcgcat tggctgtaaa gtatgcttac ttgattaagt
    49201 aaaggagaat ctacatatgt atgatgtaat cgttattggc gcaggtcctg ctgggatgac
    49261 tgctgcttta tatgcttcac gttcaaactt atccgtattg atgatcgaac aaggcgcccc
    49321 tggcggccaa atgaacaata cggcagaaat cgaaaactat cctggtttcg attcaatcat
    49381 gggtcctgat ctagcttata agatgtatga aggtgtttca agatttggga cagaaaatgc
    49441 ttatggaatc gtgcaagata tcaaggatca cggcagccac aaagaagtta tttgtgaaga
    49501 taaaacttac gaaggaaaaa cagtcattat cgcaacaggc tgtatccacc gtaaattagg
    49561 cgtacctggc gaggaagaat ttgctggacg tggcgtttct tactgtgcag tttgtgatgg
    49621 tgcgtttttc cgtaacaaac gtctagtagt gatcggagga ggagactcag cagtagaaga
    49681 agccatctat ttgacacaat ttgcttctga agtagtcatc gtccaccgta gagatgaatt
    49741 acgtgcacaa aagatcatcc aagaccgtgc atttgcgaat gaaaaaattt cctttatgtg
    49801 ggatacagtc gttgacgaaa tcgttgggaa tgaaatggta gtgacaggtg tgaaagcacg
    49861 taatgtcaaa accggcaatg agtttgattt ggcagcagac ggagtattca tctatgttgg
    49921 actggatcca ttaactgcac catttagaga agcagggttg acgaacgaag ctggctggat
    49981 tccaacagac aacgaaatga agacgaatat accaggcgtt tttgccatcg gagatgttcg
    50041 tgaaaaagat ttgcgacaaa tcactactgc tgttggtgat ggtgggatcg ctggacaaca
    50101 agtgtataaa tacttggaat ctttagaaga aacaactgca taagtttaaa aagagtggaa
    50161 gaacggtttc aattgttctt ttactctttt ttttcgcgtg aaatctactg aatccgattt
    50221 atgcgaatac ttgcaaccgt gcttgataaa agaatccttt cttgatttaa gcgtttttta
    50281 gacctcgtac tttcatatct aataagtgaa tgctatactt attaagaacc atatttcaga
    50341 aagaggtgtt attttcatgt cttgggaaca agtgtatgaa caatgggtac atgaagaaaa
    50401 tttagaagaa aacttaaaaa aacaattaat tgatctgggt gaagatcctg aaaaacttga
    50461 agatgctttt tatgcaccgt tggaattcgg tactgcaggc atgcggggaa tcttaggacc
    50521 tgggatcaat cgaatgaaca tctatacggt aagacaagca acagaaggac ttgcacgttt
    50581 catggataca caaggtgaag aaacaaaacg ccgaggtgtc gctatcgctt atgactcacg
    50641 ccatcaatca ccagaatttg ccatggaagc agccaaaaca ttagcacatc acaatatccc
    50701 ttcttatgta ttcgaaagtt tacgtccaac accagagctt tcttttgcag tccgtcattt
    50761 gaagacgttt gcgggaatca tggtaactgc atcccacaat ccagcagctt acaacggata
    50821 taaagtctac ggagaagatg ggggacaaat gcctcctgca gacgcagatg cattgacgaa
    50881 atatgtccgt gaagtcagca atccgttaaa agttgaagta ttatctgacg acgaagctga
    50941 acatagcgga ttgatcacga tcatcggaga agaagtggat gccgcttatc tagaagaaat
    51001 caaagcagta acgattgatc atgaattggt ggaaacaatg ggtaaagatt tgaaacttgt
    51061 gtacacacca cttcacggaa caggaaaaat gttgggcgaa cgtgcgttga aacaagctgg
    51121 attcgaacaa ttcgtactag ttccagaaca agctgtcgca gacccggatt tcactacagt
    51181 caaatcaccg aatccagaag aacactctgc tttcgagtat gcgatgcgac ttggcgagaa
    51241 agaaggcgct gacttactga ttgcaacaga tcctgatgca gaccgtttag gcgcagccgt
    51301 tcgtttgcca aatggctcct atgaagtatt gacagggaac caaatcggag ccctgtttgc
    51361 tcaatatatt ttggaagcac accaatcgcg aggcacttta cctgaaaatg cagcggtatt
    51421 gaaatcaatc gtttctagcg aattgcctac agcaattgcg gaacactatg gggctaccat
    51481 gttcaatgta ttgacaggat tcaaattcat cgctgaaaaa attcaacaat ttgaagaaga
    51541 tcattcccat acttttatgt tcggattcga agaaagttac ggttatttag ttaaaccttt
    51601 cgttcgcgac aaagacgcga ttcaagcatt gctcttatta gcagaagtag cagcttacta
    51661 taaaaaacaa ggcaaaacgt tgtatgacgg cttgcaaacg atttttgaaa catatggcta
    51721 ctacgcagaa aaaacgattt ctgtgacaat gagcggacaa gaaggttctg caaaaatctc
    51781 tgctttgatg gaaaaattcc gtgaagaagc accgacagaa tttgccggag caaaagtcat
    51841 ccaaacagaa gacttcaaag agttgacgag aacagaccgt gaaggaaacg tagaaaaact
    51901 aacgacaccg ccatctgacg tattgaagta tacgctggaa gaaaatggct ggatcgctat
    51961 ccgtccttca ggaactgagc cgaaaatcaa attttatatc ggtgtaaaag gaaactcttc
    52021 agaagacgca gctgaaaaag tagccgctta tgaagcgatt atcaaaaaaa tgacagaaga
    52081 ataaaaaaac ttgagactga tggaaaaagt ggtaaacttt agaagtcagt cttttttcaa
    52141 gttggaggtg aatggattgc cagacacgca aaaaacaaaa gtagagaaag tcagccaact
    52201 attcaaaatg ttgagtgacc ccactcgtct caaaattctg ctgtatctga aagacgggga
    52261 acaaaatgtg acagcaatca cccaagcagt agaaatggaa caatctgctg tatcgcatca
    52321 actgcgttta ttaagagaaa accatgtcgt caagtcccat cgtgagggaa aagcgatttt
    52381 gtatagtctt gatgattctc acgtactgga tattttgaac cagacgctca aacatgtgga
    52441 acatcaataa cgataggtgc aaatagagac aaactggact gtatcaacga atcttcttcg
    52501 ctgacacagt ccagtttttt taagaaaaag acaagaaaaa ctgttagtct gtttcttcgg
    52561 tggtttcagt tccataataa ctatgatctt ctaggtctaa agcattcaaa ataagtgaag
    52621 ctgtttcttc gatagataaa gaagcgacat tgattactac gcagcccaat ttttcataaa
    52681 gatcattcgc aaaatcgagt tcagctcgta ttttttcgat atcggaataa gcagtatcag
    52741 gatttaatcc gtaagagcgc atccgttctt tacggatatt gtttaaaata tctgggttat
    52801 tcgtcagtcc cacgattttt ttaggatcga tctcccaaag ctgtttggga atatgtgctt
    52861 gtgggatcaa aggtagattg gcaaccttta aatttttgtt agctaaaaac aagctcaaag
    52921 gcgttttgga agttctggat acgcctagca aaacgacatc tgcttctaga aacccacgag
    52981 gatctttccc gtcatcgtat tttacagcaa attccattgc tttgatccgt ttgaaatagt
    53041 tctcattcaa taaatgaagg gcgccaggtt gccgaatggg cttgatacca gttaagcgct
    53101 cgatttctga tatcggcgga gtcatcacat caaaactaaa taattgatgt tcttcacaaa
    53161 aagaacgcgt catctcgatc aattcttgat tgatcaatgt gtgcaggaca agggcttttt
    53221 cttttagtgc atcttgcagt gcctgcatca atgttttctc atctgtcaca aatgttctgc
    53281 ggaataaatt gaactcaacg gtaggatatt gggccatagt ggcttgcgct aattttgtcg
    53341 cagtttctcc agcagagtca gagatgacaa aaaaagtaac gatttctgta gaatcggaag
    53401 acatggataa aacctcactt gcattttttt tatgtttatt ataccatact ctagaatgtg
    53461 tacttcgtaa tcattttgat ttggaggaat aagtcatgaa aaaacagaca ttgattgaac
    53521 aatcactaga aacattgaaa aataacggtt ttaaatatac taaaaaaaga gaagcattgt
    53581 taacttattt agtgaagcgc aatcgctacg tatccgctaa agaagttttt gactatatga
    53641 atcaggaatt caaaggagta agttacgata cgatttatcg aaatctccat gattttgcag
    53701 aaatcgattt actggaagaa accgatttaa atggagagat gaagtttcgt tttcattgct
    53761 gtcaagggga aatcggccat caccatcact ttatctgtac ggtgtgcgga acaacgaaag
    53821 aaatccatat gtgtccaatg aattatttcg aagaacagct cggtggatgt acgatcgaag
    53881 ggcatagatt cgagattttc ggaagatgtg aaaattgtca ataaggattt aaataaaata
    53941 acatttgata attttaaaaa aaagcaagat ttccttgacg gtcaaaacag cattagatat
    54001 aatatttaca ggacagtctt gttttttgag atatatttca ataacaatta cggttacttt
    54061 ttaagctcgg agggagggaa ttaacatgtc aaaaacagtc gttcgcaaaa acgaatcact
    54121 tgacgatgct cttcgtcgct tcaaacgttc cgtgtcaaaa gcgggtactt tacaagaatc
    54181 tcgtaaacgt gaattctacg aaaaaccaag tgtaagacgc aagaaaaaat ctgaagcagc
    54241 tagaaaacgc aaaaaattct agttctgtga tgaattggag ttggtagtgt gtcactacta
    54301 agtagattaa acgatgacat gaaaacagcg atgaaagcga aagataagga atctttacaa
    54361 gtcatccgaa tgatcaagtc atctatacaa aacgagcaga ttaaagaagg tcacgacttg
    54421 acagaagaag aagaattaac ggtgttgtct cgcgaaatga aacagcgacg tgattctttg
    54481 catgagtttg aagaagcagg acgtgacgat ttagctgaaa aagtaaaaag tgaaattgtg
    54541 atagtcgaaa aatacatgcc ggaacaactt tccgacgaag aaattcgaca acttgttcaa
    54601 gaagcaatca cacaaactgg tgcttcatca atgaaagaat ttggcaaagt catgggtgcg
    54661 atcatgccta aagtcaaagg aaaagcagat ggaaaccaag taaatgccat tgtgaaagaa
    54721 ctattacaat cttaatttta tcatttaaac tgcggacaat cattgtccgc agtttttttg
    54781 tgcgcgtaaa aaagagaaca aaaaaatagc tagacgaaag aataggattc tttcatctag
    54841 ctggtttttt cctgtttaat atttcttatg ttaaattgtg tttgatttgg ttattcttct
    54901 ggtagttcat ttgcttcttg tttataccct gataggcgat aggcgtaata aaacagtagg
    54961 ctgccagcgt aaccaagtaa cgttgggacc atccagccaa gtcctaatgc agcaaaagga
    55021 acgtgatgat gataaaattc gagcatgctt gctacaactc tttgttgact cactaaagca
    55081 ggcgaatttg ccagcatatc caatacggca ggaacagcag caaacaacat cgttacttga
    55141 tagacaaatg gtgtcttttg gaaaaatttt gctgttaagg aaagcaagat caacgcaagc
    55201 gacaatggat acaaaagcat caatactggt aaagaccatt ggatgatatt gtctaaacca
    55261 gcatttgcaa cgacaaaaga gacgaaagtc gttaaccgca gccatgtcat ataactgact
    55321 ttagggaaca gtttatggaa atcttgcgca aaagaaacaa cgagtcccat agcggtagtg
    55381 aagacaccta atgtgaccat cactcctaag aagctagcgc cgaagttacc taaataaaaa
    55441 ttagtgatct gagaaagtgc agttccgcca ttttctgata aagacatctt attcaaactc
    55501 atcgcaccga gaagaacaag accaaaatag atcatgactt ctagcgcaac gctcaggcta
    55561 ccagcttttg ctgttaattc tgtgactttt ttatctttga aaccaaggtc acggactgca
    55621 tggacaaaag tgacacttaa ggcaagcaag gctactgcat caaccgtgtt ataaccttct
    55681 aggacaccat ttgttaaagc gtttgtttgg taagtatctg cagcagattg atttagattt
    55741 cccattgggt ggaataaaga aacgacaaaa atgattgcta acaatgcaag aaacaaggag
    55801 tttagatact ttccgacgta tttgaccaac cgtgtgggat ggacagtaag gaaataagca
    55861 agtaaaaaga aaagagcaga gaataaaaac atacctaatg aattatattt ttcaggcaaa
    55921 aatggtttag cagccatttc aaaagcagta gcagctgtcc gtggtgtacc gaaaaatggt
    55981 ccgatcgtca aatgaacaag gattaagaac aaggcagcat accattttcc gacaggttta
    56041 gctaagtcat acagaccatc actttttgtc actactacag ctaagatagc tagtaatggg
    56101 aaaagagcgc ccgagatggc aaatcctacg ccagcactga cccagtttgc gccagccatt
    56161 tgacctaagt ggacaggaaa gattagatta cctgacccga agaacatccc gaagataagt
    56221 gaactgacca tcaatagttg tttccatgaa taagtttttg tttcttccat aaatttttcc
    56281 tccaataatt atcattttga ttgacgatat aaacgttgca gcaattagta atgccactaa
    56341 tatcagattc catgaaaacc accaccttaa aatataaaaa aagccctttt ccacttggga
    56401 aaagggctct agattctaga acggtaccac cttttttcgc ttaaaaaagc ttcttcattc
    56461 acggacgctt tttgataaag ttatccgcta gcctgataat ggaggcagcc acaatgatct
    56521 acgttttcga cattgactcg gaaatgatgt tcgtctattc gatttattgt catttctcac
    56581 ttacatgact cactgggaat aaatcgttga atagcctact gttttcctcg acgctttgct
    56641 gtatgtttat ttgttgacgt aataatacac ctgttttttt tgaaatgcaa gaatatttta
    56701 actattttgt tttctttttt tgaaacaaga aaaataccta gagaatacct gctgaatgaa
    56761 acggaatatt ttgttctcca ctttcatata acttacattc tcagcttctt tttatcaaaa
    56821 cgagaccttt agcgaatgga aacggctatt ttttaacgtt tcttggtacg tatggttaaa
    56881 cgactctttt acaacctttt cttatttgct atatagtttc ttctgagtat ggtaaaatag
    56941 gaaagaatga atcgaaaaaa aggggatgtc tatttttgac ggaagagtct agttctttag
    57001 agattagatt gacagctgct gatgatgtca gcatgctttt aggtgcacac gacaagcata
    57061 taaaactgat cgaggaaaca acggaaacaa ccatccatac acgtggagaa atcattcaga
    57121 tcattggtga gcaatcccag atttccttgg ctgcctcagt cattcggaca ttacaggaat
    57181 tgatcaaacg aggaatccac gtaagtacgc cagatgttgt gacggcattg aagatggggc
    57241 aaaaaggtac attagattat tttgttgaga tgtatgagga agagatcgtc aaagaccgta
    57301 atggaaaacc tatacgggta aaaaacagcg gtcaaaagaa atacgttgaa tcggtagcca
    57361 aacatgacat cgtatttggt gtagggccag ccggaacagg gaaaacgttt cttgccgtag
    57421 tattagcaat tgctgcccta aaaaaaggcg aagtgcaaaa aatcattttg acgcggcccg
    57481 ctgtggaggc aggggaaaac ctaggctttc tacccgggga tctgaaagaa aaagtggatc
    57541 cttatctgcg gcctgtttat gatgctttat accaaatttt tggattggat catacgaacc
    57601 gattgatgga acgaggcgtg atcgaaatcg cacctttagc ttacatgcgg gggcggacat
    57661 tggatgatgc attcgttatc ttggacgaag cacaaaacac gacagtcgct cagatgaaaa
    57721 tgttcttgac ccgtctcggt tatcaatcaa aaatgattgt caatggggat acaagtcaga
    57781 tagacttgcc tcgtgggacg acaagtggtc ttgttcatgc agaacgaaca ttaaagcaaa
    57841 tcaaaaaaat cgattttgtt aattttgaag caagtgacgt tgttcggcac cctgtagttg
    57901 ccgaaatcat caaagcttat gaaaaggcgg atcttcatca agaataagct ttgcttagga
    57961 ggataagcac atgttgaata aaacactgca gaaactacaa aaaaaactcg gaaaggcagc
    58021 attgcctttc cttctgttct tgttttttat cattgtctgc ggcgttacat ttagtagtgt
    58081 caaacaaaag gccaatgatt tcagagaagg ccaagtagca gaagagagta tccgtgcgaa
    58141 taaaacgatt gagaacacgg aagaaaccga acaaagaaga aaactggcag cagaagcagt
    58201 gactccagaa tatacttatc aaaaagatct ggcagatgac cagaacaatc ggatcaagca
    58261 attattcgaa ttgatcgata agacgaatga taccatcaac aaatcttata atgacaaggt
    58321 agataaggcg aaggaagatg aaaatatccc gaaacctact acagaagagc ggattgctac
    58381 actgaagaaa aattttgaaa acgtgaatgc agaaaacgtt gcgttttacc aaaaactgcc
    58441 gaacaatttt tattcgacga tatttgaaat gactgaaacg gaaattcaca cggttaggga
    58501 tgaaagtttg aagctcattg acgaacagat gagtaaacaa gttcgtgaaa gcgaattgga
    58561 agcttttaaa caagaagcag aagatcagat tcaatatttg aatgtcacaa cagagcagca
    58621 acaaatgatt cgctacttag tggatcaagg cattgtagtt aatgatgtat tgaatgagaa
    58681 aaagacagaa gaattaaaac aatctgcacg agaagctgta caaccagtga tgatcttcca
    58741 aggagaaatt attgttcgcg aaggaaatca aattgatgct tcagcgatga aaaaattgga
    58801 attactagga ttgacgaatc aaacgacttc tattttccca ctcattgcca tggttttagc
    58861 tgttttgctt caaatcggtg tactgattta taattcactt caattccaaa atgcaggaga
    58921 aagaacaaaa tacgtacttt tctacgtgac ggcaatgtca gtaagcgtcg tactaatgaa
    58981 atttttccaa ctcttccaaa cagaacaagc tgcttatatt ccgctgtttt accccgcggc
    59041 ttttgctcca cttgttttaa gctttttctt gaatcgaaga gcagggatca tggctggtat
    59101 ttttcaagca gtatccgcac ttttcatttt ttatgattcg attggcacga actttctgac
    59161 agttattttg atgtcctatg tattttctgg tctcatggca acgatcgtca gacgcaaacg
    59221 gatctcagaa caagggttat ccgctgctat gtgggtcatt atttttcctg tgatgatgga
    59281 tgtcattttg attatctacc aaggaatgag ctttggggat gcaacgactt ggttaacgct
    59341 agtatgcgga ttcacaggtg cactgttctc attcttgctg acgataggtc tgcaccccta
    59401 tattgaattg atggtcaatg atgatagtgt gatcgtatta aatgaactca gcaatcctaa
    59461 tcatccttta ttgaaacagt tgctggaaga agcaccaggc acgtatcacc atagcatgat
    59521 ggtagccaat ctaagtgcta atgctgtagc agaaatcggt ggacgttctt tactaactcg
    59581 agtagcttgc tattatcatg atatcggcaa gatcaaacac gccagctttt tcgttgagaa
    59641 cttgccttca ggagcggaaa atccgcacaa tttcttattg ccagaagaca gtaaacatat
    59701 tatctttgga catgtgacag atggtgccaa aatattggaa gattataata tgccgcaaat
    59761 ggtgatcgat atttgcagac agcatcatgg aaccacgctg atgcgttatt tctacgttaa
    59821 ggcaaaggaa cgcaacccgg aagtcacgga agagcaattc cgttatcctg gaccaaaacc
    59881 tcaaagccgt gaagcaggaa tcgtcaatat agctgacagt tgtgaagcgg cagtacgagc
    59941 aatggaccat ccaacgagcg aaaaaatcga aagttttgtc cataatctta ttgaagagcg
    60001 tatttccgat ggacaattgg acgacagtgg attgacgtta aaagaaatcc gtaaagtaga
    60061 aaaatcattg atcagcggtt taagcagtac cttccattct cggatcaaat atcctaaaat
    60121 gaaatcggaa gttgagaaga taaaagaaga acaagaagaa tagaaagaag cgatgtataa
    60181 tggatattac tttcatggat gaaacaaacc aagtctcaga agagaaaatg aaagaaatcg
    60241 acgacttgct gcaatttgca gcatcatatt tagagttgcc agaagaaaca gaaatgtctg
    60301 ttactttcat ggacaatgca gcgatccaag taatcaatcg tgattaccgt ggaaaagatg
    60361 caccgacaga cgtcatcagt tttgccttgg aagaagaggg cgaagacgaa atcccagtga
    60421 tttttgaaga tggagagaat ccattgcctc gtgtactggg agacatcatg atctctactg
    60481 aacgtgctgc agagcaagca gaagcttacg gccattcgta tgatcgggaa cttggctttt
    60541 tagcggttca tggattcttg catatcaacg ggtatgacca tatgacacca gaggatgaga
    60601 aagaaatgtt tggtttacag aaagagatct tagatgccta tggacttaaa agataaaaac
    60661 gtagagaaaa acaaacattt cctgatgtcc gtggaatttg ccgtccaagg agtcaaaacc
    60721 gtttttgagg aagaacgcaa catgaggaaa catgttgcgt tcggaatcct cgctttgttt
    60781 atgggcgtga ttttccagct tgatcggata gaatggttat ggctgctttt agctgttttt
    60841 ctcgtatgga tagtagagat catcaatact gtttttgaaa atgtcgtgga catgtttacg
    60901 gattttcatt ttcatccgat tgggaaaaaa atcaaagata tggcagctgg agcagtttta
    60961 gtgaccgctt gtttttccgt catcgttggg ttgattttat ttgtccctaa aatatatcat
    61021 ttgttgttta attaaaaagg agaacgtata tgccagaaca taaatcaggg tttgtcgcaa
    61081 tcgtcggccg cccgaatgta ggaaaatcca cattattgaa tcgaatcgtg ggtcaaaaaa
    61141 ttgccatcat gagtgataag gcgcagacaa ctagaaataa aatccaagga gtatatacta
    61201 caccagaagc tcagttgatt tttattgaca caccaggaat ccataaacca aagcatcgtt
    61261 taggagattt tatggtagaa gcagcttaca gtgcgttgaa agaagtggac gcggtcttat
    61321 ttatgatcag cgctgatcaa aaacgcggtc ggggagacga ttttattatt gaacgtctaa
    61381 aaaacgtgca ttctcctgtt tatctgatta tcaataaaat cgataaaatc catccagacg
    61441 agttgttggg aatcatcgaa gattattcaa cgcagatgcc gtttgcacaa gtgattccaa
    61501 tttctgctac agaaggaaac aacgtcgaac gtttgatgga tgtattagtc agtgaaatgc
    61561 cagagggccc acaatatttt ccagatgatc aagtgactga tcatcctgaa tacttcatcg
    61621 tttctgaatt gatccgtgag aaagtcttgt ttttaacacg agacgaagtg cctcattctg
    61681 tcgcagtcgt tgttgattcg atgaagcgaa acgagaatga taaaatccat atccaggcta
    61741 cgattattgt cgaaagagat agccaaaaag ggatcattat cggcaaaggc ggaaaaatgc
    61801 tgaagcaagt aggaacgaaa gcgagaaaag atatcgaaaa tcttttagga gacaaagtct
    61861 ttctagaact ttgggtcaaa gttcaaaaag actggcgaga caaaaaagtc tatctgcaag
    61921 acttcggcta ccgaaaagac gaatattgat attctgagct tcttgtaagt taggtataga
    61981 aataaactgg ataaaaatcc gcgtgttgag aaaagctgcg gaagatcgca acataaatga
    62041 tctggatcgg cagaatctgc ttttccagat ttatttttta ggagggagaa tatgagccag
    62101 cttgcagaat caaaaggctt gattttatct agccgtaatt acaaagaaaa agataaatta
    62161 gtcaaaatct ttacggaatc ttctggcaaa atgatgtttt atatcaaagg gatccatcgg
    62221 caaaatcagc ccttagctcc ggctttgctc ccttttactg aagcagttta tatcgggaat
    62281 tttcgaagtg aaggtttgtc atttttaagc agtgtcaaag atgtccacgc gtttcgacac
    62341 atccaagaag acatcttttt gagcggctat ggcacatatt tattgaatct agtagatgcg
    62401 gcaatcgaag atcatcagta cgacccgcat ttgttccagt ttacgcaaca agcactgcaa
    62461 ctgatggacc aaggagatga tgcagaaatc atcacgaata tttttgaagt ccaaatactg
    62521 caacgcttcg gaattgcacc aatctggaca cactgcgtcg tatgtgggga aacaaaagga
    62581 aaatttgact attcttccaa atatggtggt gtgatatgcg aaagacactg gtcgatggat
    62641 gatcaccgtt atcatgcaga tcctcgagct gtttatttcg ttcggatgtt ttcagctatt
    62701 tcttatgata aaatcagtgg aatccatctg aaagaagaaa ccaaacaagc aatccgtgaa
    62761 ctcatcgacc agctgtatga tgaatatgtc ggtatccatt tgaaaagcaa aaagttcatt
    62821 gatcaaatga agtcttgggc acacgtatta aagacgtcag agaaagacat gtctgaaaaa
    62881 gatctaccta aaaaagaaga atagtaaatg aaacatacaa caaaaaatca aaaccatttg
    62941 acaaacacag ggagttccgt ctattattaa ccgtagatca aatagatcgt tcactgagag
    63001 gatctattat agaaacaaga aaactgcaaa atgaatacgt acattgacca agatcagtaa
    63061 aaaacacgtg gtttagcgag ttcgggagag tgggagccgg acccaaacga tttttgaaga
    63121 ggcacttgcg agtacgataa atgaagctgc cgaatcttta ttcggaagta gggtggaacc
    63181 gcgaattatt cgtccctatg tctgtaatca gacataggga ttttttgtcg ttttctttcc
    63241 tgtattctta tttgacagca acgctttaaa attgaaaggg gcaaaacaga tgagtaaaaa
    63301 attgaccgtc caagatatga ttttaacatt acaaaaattt tggtcatcaa acggctgcat
    63361 gctgatgcaa gcgtatgaca cagaaaaagg agcaggtacg atgagcccat ataccttttt
    63421 acgggcaatc ggaccagaac catggaatgc tgcttacgta gagccatctc gccgaccagc
    63481 tgatggacgt tatggcgaaa acccaaaccg tttgtatcag catcatcaat tccaagtcgt
    63541 gatgaaacct tcacctgaga atatccaaga gctttattta gaaagcttgc gccttttagg
    63601 aatcgatcca ctagaacacg acatccgttt tgttgaagac aactgggaga acccatccat
    63661 gggctgtgcc ggtgtgggtt gggaagtatg gcttgacgga atggaaatca cacaattcac
    63721 gtacttccaa caagttggtg gactgccttg taaaccagta acttctgaga tcacttatgg
    63781 attagaacgt cttgcttctt atatccaaga agttgaaagt gtctatgatt tagaatgggc
    63841 tgatggcgtg aaatatggcg agatcttcaa acaaccagaa tatgaacatt ctaaatattc
    63901 ttttgaagta agcgaccaag acttgctact aagcaacttt gatcgttttg aaaaagaagc
    63961 taaacgctgt atcgatgaaa atcttgttca tccggcgtat gattatatct tgaaatgtag
    64021 ccatacattc aacttattag acgcccgcgg tgcagtatcc gtaacagagc gtgccggtta
    64081 tctttctcgc atccgcaaca tggctcgtgc tgtagccaaa atctttgtgg cagaacgcga
    64141 aaaattagga ttccctttgt tgaataaaaa cacagcaaat ccagaaaacg tgaaggaggc
    64201 tgaataagat ggcaaaagac ttattacttg aaatcgggct ggaagaaatg cctgctcatg
    64261 ttgtcacacc aagcatgaaa caattagaag aaaaaacagc aaaattttta gatgaaaacc
    64321 aattgactta tgatacgatc gaaacatttt ccacaccgcg ccgtttagca atcaaagtaa
    64381 caaacattcc agaacgacaa gcggacagtg aagaagaagt caaaggacca gccaaaaaaa
    64441 ttgctttgga cgcagatgga aactggacga aagcagcgga aggatttgtc cgcggacaag
    64501 gtctgacgac agacgctatc acttttagag aattaaaagg tgtggaatat gtctatgtaa
    64561 cgaaatacac tcatggtaag gaatccaaag aagtattgac aggtttagca gatgtcatta
    64621 aaagcctgac tttccctgta acgatgcatt gggcagaata cgattttgaa tatatccgcc
    64681 ctattcactg gatcgtggca ttgttagatg acgaagtaat cccattcgaa atcttggatg
    64741 tcaaaacagg cagagcctct cgaggtcatc gtttcttagg cgaagatgtg acatttgcgc
    64801 atgcaaaaga atatgaagaa aaattgaatg aacaattcgt cattgcgtct ccgcaaaaaa
    64861 gacaagaaat gatcgttaca caagccaatg aattagccga aaaaaatcat tgggttattg
    64921 atctagacga agatttacta gaagaagtca acaatttagt cgaatatcca actgtctttt
    64981 ctggcgactt cgaagaaaaa tatctttctg ttcctgaaga agtactagtc acttctatga
    65041 aagaacatca acgttatttc gaggtacgga atgatcaagg tatgctgctt cctcatttta
    65101 tttctgctcg taatggtgat cgggtaaaat tggaaaatgt ggttcgaggc aatcaaaaag
    65161 tattgacggc tcgtttagaa gatgcggaat tcttttataa tgaagacaag aaagcttcaa
    65221 tcgaccaata cgttgaaaaa ctaaaaactg ttactttcca tgaaaaaatc ggtagtatct
    65281 atgagaaaat gcaacgggta caagtcattg cccgtatcat tggcgaacaa gtaggactat
    65341 ctgaggaaga attgatggat ctgcaacgag ctgctgaaat ctataaattt gatctagtaa
    65401 ccaacatggt aggggaattt cctgaactgc aagggatcat gggcgaaaaa tatgctctgc
    65461 tgaaaggcga gaaacctgct gtagcgacag cgattcgtga gcattacatg cctatatcca
    65521 gtgaaggaga gctacctcag tctacagtag gttctgtttt agcgattgca gataaattag
    65581 atagcatctt ttctttcttt gctgttggga tgatcccaag cggttcaaac gatccttatg
    65641 ccttacgtcg ccaagcatac ggtgtcgttc ggatcatcga atcgaaaaaa tgggcctttc
    65701 ctttgtctgt tctgcaagaa acgattagcg atgttatcag taaagatacg gatcgtttcg
    65761 gcattggttt atcagaaggg caacaacaag tgatcgactt tatcaaaggc cgtctgcgcc
    65821 aattattgac aacgaagaat attcgtcatg atgtcatcga tgctgttttg aatgcggaac
    65881 aaaaagatct gacaaaagta tttgcagcag cacaattgtt caaacaacat cttgcagatg
    65941 aagacttcaa accttcaatg gaagctttga cacgtgtcgt gaacttagcg aaaaaagcgc
    66001 agcttgaaaa acaatcagaa gtagatcctg agctgttcga aaacgacgca gagaaagaat
    66061 tacacaaagc agtagaagca atgaaagcaa gttttgctga ccaaacgatc cacgaaaact
    66121 acgaccagct tgtcgctctt cgtccactga tcgaaaatta ttttgatcaa acgatggtca
    66181 tggctgatga accagcagta cgtaagaacc gactaacaca acttagtcaa atagctgcca
    66241 tggctttgtc attagcaagc ttggatcaaa tcatcacaaa atagtctttt gtttgaaaaa
    66301 cagagagcat gcacgaaacg tgctgtgtct ctgttttttt gttataaata taaatagctg
    66361 ttattaattc tattttaata atttttgtta ttttattctt tgttcgaatt ttcaaaatgc
    66421 tcatgaaaag agaggaaata aaaatgaaaa cgattaatca tgatcttgtt actgttttat
    66481 ttacttttaa attaggaatt tccctagtca agaaaacttt ttgtggtatg attatatagg
    66541 gaaaacctgt ttataaatat ttctatgaat atattagttt aattatgctt ttaaaataaa
    66601 aagcgctcat tctgatggga atgaatcaca tctttattta tctatcatca atatttagga
    66661 gtgaatctat ggaaaagccg ttcgtcaagt tattggctac tgtggcggta ggcacaggcg
    66721 ctattgtgat ttgcttgttt ggataccatt tcaataatca gcggcaacat catcaacgga
    66781 tcaattacgc agaatctgcc atcaccaatc aaaaagacac cgtgacctct ttatcaaaag
    66841 aagtagataa gctgtacagt acaaaagaaa aaattttcct aaatcctgaa ataacagaag
    66901 aaacagtttc aaatttgtca cataaactat cttctatcaa actttcggca gatgattatg
    66961 acatcaagga atcagaatta cctaaagaag ctgctgcgat ccaagaagaa aagaaagcag
    67021 tcttaacgca gttagaggat gcagaatcaa aactgaagat ccagacagct gtcaacgatt
    67081 tatttactaa aaatgtatca aactggcagc aagctgtaga cgatgtcgtc atcaaagaaa
    67141 aggtgacaag tgcagatgtc gctcgtgtac gtgaaaatat gagctttttt aaagatagtc
    67201 cttggaaaac ggttgttatg caatatctag gattcgctga tactcagatc gcgcaagtca
    67261 cgcaactaga tcagctattc gatacgatgc tgaaagacgg tcaagtaaca gctactgcta
    67321 cttacgatca atatttgacc gcactcagtc tcatcgaaca gatccggaat gaaaaaataa
    67381 gagccgcata cgcgtcaaaa gcagaaacag tcgctcagca aatgggctat agcaatacga
    67441 gctattaata agcgagaatt caagcaaggc tagcaaacgg aggaaactat ggcaaaacga
    67501 agaagaagat caagagggaa aaagatattt ctaattattt tagcaattct tgtcgtatta
    67561 gtattagcaa tcgtaggagt aggagcaaaa ttatatctag atctctcaca ttctattgaa
    67621 aaaacttacg aatcagtgga acgaacccaa ggagaacgaa caaaaccagt tgatctggac
    67681 aaacaagaat ctttctctgt tctattgatg gggattgata cgggagattt aggtcgggtc
    67741 gatcaaggaa gatcagatac gatgatggtt gccacagtca gtcctgaaga caaacagaca
    67801 acgattgtca gcattcctcg tgatacgtat gtcgacatcg ttggacatga cacaaaagac
    67861 aagatcaacc acgcttacgc attcggcgga gctgccatgg cgatggatac cgtacaaaaa
    67921 tatgtagaca tcccaatcga tcactatgtt tcaatcaata tgaaaggtct aaaagaactc
    67981 gttgatgcag ttggtggtat cgaagtcaat aatgacctca ccttctcaca agatggctat
    68041 gattttacga tcggtaaaat cacattgaac ggggatcagg cattgtctta ttctcgtatg
    68101 cgttatgaag atccaaatgg tgactacggc agacaagaac gtcaaagaaa gatcgtagaa
    68161 ggtgtagcga aaaaagtgtt gagtctgaac ggtgtaagca actatcaatc cgttttgaat
    68221 gctctagagt cgaatatgaa aacagacatg agctttgatg aaatgaaaaa gattgcttta
    68281 gattatcggg atgcatttgg tacgatcaag caagatcagc tgcaaggaga aggcttcatg
    68341 caagacaata tctcatacca aaaagtcagc gatcaagaac ttgatcgtgt gaaaaaagaa
    68401 ttaaaagatc aaatgaatct agagaataaa taggaaagtg gagaaaaaat ggaagagaca
    68461 atcagcttga aagaaatttt tgaaacatta cgcaaacacg tgataacaat cctcatctca
    68521 atgtttgctg gcttggccat ctcaggcatc gtgacgtttt tcatcttgac gccaaaatac
    68581 agctcacaag cccaattgat cgtgacactg ccgcaatcag atactgcaaa tgtcaatgat
    68641 gtcaatacga acctgcaaat gatcaatacg tataaggata tgatcgtcag tgatctagta
    68701 ctaaatgaag tcaaagatcg tttagaaaca gaagacaatg taaaaatgac tgctggacaa
    68761 atcaaagatg ccatctccgt caatcagtca caaaattcac aaatgttctc gatccaagca
    68821 acaagcacaa atgcagtgac ggctcaacaa atcgctaata caacgtcaca agtattccaa
    68881 gagaacgcca aagacgtgat gaatgtcgat aaaatctcga tcatctcagg tgcagttgcc
    68941 aatacgacac ctgtttcgcc aaataataaa ttgaatctag tgattggctt agtgttagga
    69001 atgatggtag gcgtaggtct agctttctta tttgaattac tcgaccggac agtaaaagat
    69061 gaaaaatatg tgacggatac attgggcttc ccgatcttag ggactgtacc agaaatgtca
    69121 gcgaaagaat tgaatgctgg tatccgtaaa aaaggaccag ctccgacaca accaaaagct
    69181 acaaacgata aaacagcgcc atcacgcaga agccgctcaa gagtctagga ggaaagaaaa
    69241 atggcacgaa cacagaaaca aaaagcagcc caaactcatg cagtcagttt ggtcacactc
    69301 acaaacccct cttcaccaat cgctgaacaa tatcgtacga ttcggacgaa tatccagttt
    69361 gcctccgcag ctggtcagca aattaaaacg attgttgtga cttcatcagg acctggagaa
    69421 gggaaatcga ccactgcagc aaacattgcc gttgtttttg ccaaatctgg tcaacgggtg
    69481 ctgctagtcg atgcagactt acgtaagccc gtcatctata aaacgttcaa gttgaacaac
    69541 gcaagcggat taagtaccgt tcttagttca tcaggaagtg tagcagatga gatccaacga
    69601 acaccagtcg agaatttgtc gattctgcca agtggaccta aaccaccaaa tccatcagaa
    69661 ctactaagtt cgccaagaat ggatcagatt ttagcagaag caagacaact atttgatgta
    69721 gtgatcttcg acatgccgcc agtagttgcc gtaacagatg cacagatcat gtcttccaaa
    69781 acggatggca ccttattggt cgtacgtgaa aatatgtcac ggaaagaatc attgactaaa
    69841 gcaaaagaat tgctggatat ggtacaagcg cgagtccttg gtgttgtata caatggggca
    69901 gaacacagca aagatgctgg ctattactac gggaattaag aaataaaaag agaggagaca
    69961 agagaaatga tcgatctaca ttgccatatc ctgccaggaa tcgatgacgg agcagaaaac
    70021 atagatgcca gtatcgccat ggctgaaaaa gcgattcaac aagggatcac acatatttta
    70081 tgcacacccc atcataataa tggaaaatac agcaatgaaa aatcacaagt catttctcta
    70141 gtcgcttcgt tacaagcaga attagagaaa agacaattgc ctttgacgtt attggaagga
    70201 caagaagtac gtattaccgg aacactgatc gaagacatcg accgagatga aattttattt
    70261 acagatttag atgatacata tcttctaatc gaatttccga cactggaagt gccgttatat
    70321 gcagaacagc tcttttttga gttgttacaa ctaggaaaga caccagtgat cgtccatcca
    70381 gaacgcaacg cgcattttcg taaagacccg aatcatatgc tgccattcct agatatggga
    70441 tgcttggccc aactcaccgc accaagttat gtcggtgtat ttggaaaaga catccagaaa
    70501 acagccaaaa tgatggtcaa acataatctt gtgcagatgg ctgcttctga tgcccatggg
    70561 gtctctaagc gtacctttta cttaaaagag tgctatgagc agattgctaa ggactttggt
    70621 aaggaaaaag tagtacagat gaaacaagtc gcaaaggatc tgatcaacgg ggatgaaatc
    70681 agttatccag tctatgaaga ggtaaaaaag aagaagtttg gtttatttta gttctttatt
    70741 cgtagataaa aaggaaatga ggagagaggg aaagtatgga aagtgaagat ttattatctc
    70801 gtattagtga agtacatcct aatcaagttt ggttggataa agaaaaagtt gaaaaacgat
    70861 atatctattt attttttaaa agaatgattg atattgtagg aagcattatt ggtttgattt
    70921 tagcttctcc tttgatgttc tatgtagctt atcgaatcag aaaagaagag ccaggaagtc
    70981 cgatattttt ttgccataca cgtgttggta aaaatggaaa aacattcaaa atgtataaat
    71041 tccgttcgat gtgtttagat gctgaagaaa aattggaaga attacttgat caaaatgaaa
    71101 tagaaggtgc aatgttcaag atgaaagaag atccccgtgt cactaaaata ggtaaaatta
    71161 tccgtgccaa aagcattgat gaattgccac agctatggaa tgttttaaaa ggcgatatga
    71221 gtctaattgg tccacggcca cctttggaga gggaagtagc tgagtacacg agatatgatc
    71281 ttcaacgctt gtatgtaaaa cctggttgtt caggtttgtg gcaagttagt gatagaaata
    71341 atgttagttt ccataaaatg gttgagttgg atttagagta tattagtcat cgaagtttaa
    71401 tgtacgactt gaggttgatt ttaaaaacgt tttatgttat tgtcaagccc aatggagcat
    71461 attagttagg gatagaatga tggattgtta ataaaaaaga ttacacatgt tcatattatc
    71521 gtttcaaaag taataactgt aattatggag ggtttgaatc acttgtagaa aatctaaaaa
    71581 aagaaaggtg aatggggcaa ttcagtatca ttattcttat ataaaagaaa atattcgaaa
    71641 ttttgggatg ataccactct taaatataat gatctaaatg ctttaatatt ggtatatccg
    71701 atataggaac tgacgagcta tttagtatga tacaaaagta tattgatagc cattaataaa
    71761 ttttccaaaa aataatgaca gcacaccaat ttttataatt tagcttgttg aatcggtatg
    71821 ttcatgggac ggttcaaaaa aagaatcgaa aaataaacgg taaactttta gtgaatccta
    71881 acggatatga gtggttacga gcaaaaggga gttatccggt cccaaaatat tgtaattatt
    71941 ctgaaacatt aatggaatgg tatcaactga taataacata ataagagata aaattgagga
    72001 atatctatca ttttataaca aagagaatgc tgcagagtgt ttacttaaaa tgtgggagta
    72061 aggtatttgt gactttagaa gaggctattt tttatctata agaatttatt atccactgtg
    72121 cagaaatgaa gattatagtt catctaaact tttaaacttt gtaatatatt ggatgttcat
    72181 aacggaaaat aatatttgga aaagtacgtt gttataaagt atgtaccact atcttaaata
    72241 ttatgaaact aagacaattt tttttgaaac tatctataga tatatttact ggaatttttg
    72301 gaggtataga aacagttatc attttatctt tgtaccacca atgatttata tcagctcaac
    72361 agagtgaatt gtatttacgc aagaatgcgt aggatgaaat ggatagataa ttaagatata
    72421 tatgtatatg gtgactgaag acaaaaaaac agaactaatg aaactgaata aaaatataga
    72481 gtgaactagt ttctaagagg gattttaaac tagatttcat tggtaataaa atttggacag
    72541 atggaaaaac atcacataag gaagcagatt ttgttgtcat ttttgttcct acaaactatg
    72601 gtagtgaatg tgttattgaa ttagtgatgt ctgttacttc agaggcattg gggataatta
    72661 aatccactat acctgtagga tatacaaaaa gtggaagaaa aaaatattat caatagattt
    72721 tatttagttc agaattttta cgtgaaagta gattattaat gataatcttt atccaattag
    72781 aattattatc gtaggatgtg atgatgaaag ccgtgaaatt gttcaagtat ttaccaatgt
    72841 ctccctgcca gtaaatccgc ttagaatttg agccttgaaa atgtgcaatg ataaaaaaca
    72901 aaaactcgct caaaatagac agtttggggt gggaatagac caataatgta ccaaacaaac
    72961 ttccatttgg ggtgaaaata ggtcaataaa gcgatgttaa acataccttt ggagtaagaa
    73021 tagagggtaa taagtgaatt tataaacttt ttggtgactg gttgagtgga aaatgtaggt
    73081 acatttcaat gcgaaaatgt attataaccc caaacgaggt caataaaaat gactcaaact
    73141 tgcaatataa ccctaaacgg gtaggggatt tatgaaaaat tgaatttgcc ggcgtaggag
    73201 ctggctgttt taagtatgaa tgaacacagc tttcgggctg ttttatataa atagcaggtt
    73261 actgttcaca aacagcattt tgttaagaaa gtagtgacca cagcagtggt ggaaagagga
    73321 aatattatga agaaacagta taacattgca gtcgccggca ccggttatgt tggtttgagt
    73381 attgccactc tgttggcaca gcaccataag gttacagcgg tggatatcat tccagagaag
    73441 gttgaactga ttaataaccg taaatctcca atccaggatg attatattga aaagtacttg
    73501 gctgagaaag aactgaatct gaccgcaaca ttggatgcaa aggcagcata ccagtctgcg
    73561 gattttgttg ttattgcggc tcctactaac tacaatagcc gcactcaaca ttttgacaca
    73621 tccgctgttg aggctgttat taggctggtc atggaagtaa atcctgatgc cattatggtt
    73681 attaagtcca ccattcctgt tggatacacc gcttctgttc gtgtaaagac tggtagcaag
    73741 aatattattt tcagccctga gttcctgcgt gaaagtaagg cactgtatga caatctgtat
    73801 ccttcgcgta ttattgtggg taccgatatg gatgatgagc gtttggttga ggatgcacac
    73861 acttttgcta cactgctaca ggaaggcgca atcaaagaga atgtggacac attgttcatg
    73921 ggctttactg aagcagaagc tgttaagctg tttgccaata catatctggc tcttcgtgtc
    73981 agctacttca atgagctgga cacttatgca gagatgaaag gcttgaacac tcagcagatc
    74041 attaatggag tttgccttga tcctcgtatt ggtactcatt acaacaaccc ttcctttggt
    74101 tacggtggct attgcctgcc gaaggatacc aagcagcttc tggccaacta cgcagatgtt
    74161 cctgaaaatc tgatcgaggc aatcgttgaa tctaaccgta ctcgtaagga ctttattgcg
    74221 gatcgtgtac ttcagatggc tggttattac aattacgcag aagcaaatca gtatgatgct
    74281 ggcaaagaaa agcctgttgt gattggtgtt taccgtttga ccatgaaatc caattctgat
    74341 aacttccgcc agagttctat ccagggcgtt atgaagcgtg taaaagctaa aggtgcaact
    74401 gttgtgattt atgagccgac cttgaaagat ggagagactt tctttggcag taaggttgtg
    74461 aatgatttgg aacagtttaa ggcaatgtgt gattctgtta tagcaaatcg ctacgatgct
    74521 tgccttgatg acattaagga aaaagtatat acccgtgata tcttccagag agactaagat
    74581 taggagagag tatttatgat tattacaaaa acaccgtttc gtatgtcttt ttttggcggt
    74641 ggaacggata tggaggatta tttcaaagag aatggcggtg cagttctttc tactactttt
    74701 gataagtact gttatgtgaa tgtgagacat ctgcctagat tttttgatta cagcgctgag
    74761 ttatcttatt ctcgtactga gagagtaact agcattgaag atattgagca tcctgcgatt
    74821 cgtaatgcga tgaaaatgct cgatatgcat gaaattcggc ttacatacga agctgattta
    74881 cctgctcgct ctggtctggg tacatccagt tcttttgcag ttggtatgtt gaatgctttt
    74941 tatgcactgg agggtaagta tgctgacaag aagaaacttg ctgatgaagc catttatctt
    75001 gaaagagttc tgtgtaatga agccggtgga tggcaggatc agattgccgc atcttatggt
    75061 gggttcaatc gaattaattt caatgtggat ggttatgagg tgctgcctgt cattatttcg
    75121 cctgaacgta aaaagcagct taacaacaat ctgatgatgt tctttaccgg attcactaga
    75181 ttttcttctg atgttcaaaa ggctaacaac gtcagtggca cagaggataa gagagtaaga
    75241 ctgaaaaaga tgtatgagct tgtagatgaa gctgaggcaa ttcttactga taagaacaga
    75301 aatttagatg atttcggtag acagcttgat gttacttgga gattgaaaaa aggtactggt
    75361 ggagcaatta gtacaggaag cattgatgaa ctatatgaaa agggtatggc tgcaggtgct
    75421 ttaggcggaa agttgctcgg tgcaggtggt ggaggtttct tggttttcta tgtacaacct
    75481 gagaaacagg atgcagttcg ttgggctatg cgtgatttga tgtatattcc gtttgaattt
    75541 gaaaatggtg gaactagagt tattcactac acgccggaaa cttatattga aaaacagaat
    75601 ggataaggag taacaatgtt agcggaaaaa cttaataaac atattgactt attgattaaa
    75661 cgatatccta cacttgagac gattaaacag gacattatcg atggatacct aattatggag
    75721 gaatgctatt tgaatggtgg taaacttctt gtggcaggaa acggaggatc agccgctgac
    75781 tcggaacata ttgctggcga gttgatgaag cggtttaaga cgccacgtcc tgtaagtaag
    75841 gaatttgccg ataagttagt tgcaattgac cctgtaagag gtccggaatt agccaaaaat
    75901 ctcgagtgtg gtttaacggc aattcctttg gtcgcacacg aagcattgtc tactgcttat
    75961 attaatgatg tcgatggttt gggtgtcttc gcacagcagc tgtttggctt tggtagatct
    76021 ggtgacgtat tccttggtat ttcgacgtcc ggaaatagca agaatattat gagcgcaact
    76081 gttgttgcta gggctctcgg tataaaagtt attggcttga cgggcgcaaa gggtggggaa
    76141 cttgccacgg tagcagatgt ttgtgttaag gctcctgaag ttgaaacata catgattcaa
    76201 gagtatcact tgccgatata tcattgttgg tgtttgatgc tggaagataa attctttgga
    76261 aacgaataaa atttggagga atgttaaatg aaaaagacaa ttatggttac cggtggttgc
    76321 ggtatgattg gctccaatct tgttaagaga ctggtgaagg aaggctgctg ggatgtttat
    76381 gtagctgata atctgtggag aggaaagttg gagtacctga acgacgaaga tgggcatcct
    76441 gtcatcgacc tggatactca tttcttcaat gctgatctga ctgattatga gcaggctaaa
    76501 aaggttattg gtacgactga gtatgtagtt catctggctg atgttgtggc tggcattgac
    76561 tatgtgttta agaaccaggg cgaattgttc cgaatcaata atctgatcaa ttctaatgtt
    76621 tttgattgtt gccgtaaggc tggcaaggag aaaatcaagg gtgttatcta tgttggtacc
    76681 gtatgctcct tccctctgac cagacagaat actttgaatc ctgagcctct ccgtgaagag
    76741 gagttgttcc ctgctcttcc tgagtctgct tatggctgga gcaaactgat gggccagttg
    76801 gagatggggt atttggaaaa agaaaccgga attccgtgct gtatcctgat gctccataat
    76861 gtttatggca cacctactga ttttggcgaa cgtagtcagg ttattcctgc tctgattcgt
    76921 aaagcaatca ataatcctga agagcctttt aatgtgtggg gtagcggaga gcagggacgt
    76981 gctttcattc atgtcaatga tatcgtaaat gcattggtgc tcgccctgga taagggttgg
    77041 ggtcatggtc atattcagat tggcccttct cactgtactt ccatcaagga aattgcatat
    77101 aagatcattg aaatttctgg caaagacatc aagcctttct ttgatacttc taagcctgaa
    77161 ggtgataagg ctcgttgcgc agattattct aaggcgaaag agattttgga ttgggagcct
    77221 actgtttctt tggaagaggg tcttcgtgaa tcctacaatt ggattgaaaa gcagattaag
    77281 ggttaataag tatataagct gttcttcctc cgtgtaatta gaggatgcgg aggaagaaca
    77341 tatgagtaag gatgtactga gatggaaaag aatatgtata aaagaaaacg tgatttcact
    77401 gtgtgtggaa tagtaggtgt ttgtgctact acaaagaacg agtttgtaag ggaagttttt
    77461 tccatgccaa catctccggt aagtgcgact gttgatctcg tgggagtgcc ggccattatt
    77521 tcggcaaagg aaaatcccaa aattgctgaa atgtatagcc aggctacaat ggctgcaata
    77581 gatggtatgc ctattgttaa aattggacgg cgcaaaggat ttacctgtga acgatgtgct
    77641 gcaccagata ttatgggaat ggtttttgaa gaaggcgtta gacagggaaa aacacattat
    77701 ttctatggtg gaaaagataa cgaagtgtta aataagcttc gtgcaaacct agaaagagat
    77761 tacccgggaa tcaaaattgt tggaatgtat tccccaccat ttcgtccgtt aaatgaagaa
    77821 gaggatagca agatttgcga ggaaatcaac aacttaaatc cagattttct gtgggttggt
    77881 attggtgcgc ctaagcaaga aatgtggatg tgggaacacc agaagaagat ccataacaca
    77941 gtaatgttcg gtgtaggcgc cggctttaac ttttttgcgg gcactttgga aaaagctcct
    78001 gaatggatgg aaaaagctag tctagagtgg ttatttagac tggtaaagga acctcgtaga
    78061 ctatggaggc ggtatatctt gggaggtata aagtatatct actatactat tgaatctaag
    78121 taggggggca ataaaaatag atgaaaatac ttatagtagt tacaatgacg aataactttg
    78181 gtaagaaggg gtcctataat tctcaggaaa taggattagc tcgagggtta gctgaattag
    78241 gccacgaagt tattgtatat aaaagtgtaa aaagtgatga ggaagttgga acagagcaat
    78301 taagcaatac ggcgatgatt cactatatca agtgtcgtta tgtaggatca aatggattct
    78361 tatcaacaaa gaattttgat aagaatgcgg atgttctaat acaatttgcg gaccttcaac
    78421 tatcagtacc tcatgtatac tcatggactg taaaaaatgg aataaaatat ataccttata
    78481 atgggataac gcgaagtcaa agtggcaata aatatataaa gacaattatt gaaatattgg
    78541 cgcatcgaaa ttataaaatt tataagaaaa atgggtgtgt tgccaaaaat gatcgagttt
    78601 ctgaaaaatt agtacaatta ggagtaaata aagtgaaact tgctcctgtt tgtgtggatt
    78661 ttgaacagtt acataagaat tacgaagcaa tagattttac tactctcaga gaaaaatgga
    78721 atattcctcg caatgttcgt gttattttat atgtaggacg gtttgaaaag gataaaagac
    78781 cgttagatat gattagttta tttaataaac ttgatgagca taattatttg ctgatggtag
    78841 gaactggtgc tttactcgag caaacgaagc agcagataaa tttacttgga ctgcagaata
    78901 gagtaaaaat aattgagcgt attcctaaca aagatatttg ggagttatat tgcttatcgg
    78961 atgtatttgt aaatctaaac tatactgaaa tttggggtat ggttttgcta gaggcaatgt
    79021 actatcgcct cgggattatt gctgttaatg cacctggccc acagaatatt attttggata
    79081 atagatatgg aatgttggct aaatctgacg atgaaatttt gacattctta aaagacggac
    79141 gatttaccaa cgatatcata gatgcagcat atcagcgtgt aactacagaa tttagatggt
    79201 caaagtcggg agaaattatg cttagcatgt tggaaggtga taaatgatat ggaagtaacg
    79261 aaattaatgg cattgaggaa tcgatatgct ttaaacatag ttgataattg cacgagaaaa
    79321 atagccaaaa ttcttggttg ttgtattggt aaaggtgcac agatagggaa ctctgttgaa
    79381 tttgttcata attctgtagg cactgtgatt cattctgaca caatccttga agatggtgtt
    79441 aaagtctatc agaatgtgac gtgtggttga gcagatagta atgaacgtat catcattcga
    79501 gtaaaaaaga acgctgtttt atgtgtgggt gctaaaattc tttgtaaatc taacacagaa
    79561 ttatgcgttg gggaaggtgc tgtagttgct gcaaatacag tggtattgag gtctattcca
    79621 ccctacgaag tgtggggggt acctgctaaa aagatgggga gataaaacat gaatgcatat
    79681 aaattataca aagtagaacg atggttttac ttacacaaaa tggagttttt tgccaagatt
    79741 gtaaaggcac taatttatgt acttcataat agctcaattt catatcaaac agaaattgga
    79801 gataattgta agttcttata cggtggcata ggttgtgtaa ttggaaaaga tacaaaaata
    79861 ggtaatcacg ttataatcgg aacgaatgta ctgactggtg gtagatccaa taagcctggt
    79921 atgcctacaa ttggaaacaa tgtatatatc gctactggcg caaaaatttt aggtaatatc
    79981 acaatagggg acaatgtaat agtaggggct aatgctgtgg tcattaatga tgttgaacct
    80041 aattgttcgg taggaggcgt tccggctaga gtattgaaaa aagatattga tgtaaatgat
    80101 tattgtagtt tgaatcgtta ctaaaaggag agattgtatg ttgtattttg ttattccttt
    80161 aatgagtgcg aaggcagcaa acaattggga gatggttagt accttattta accgaacatt
    80221 atggtcgtgt tataatcaga ctgatcctga tttcaaaatc attgttgctt gccatgaaat
    80281 tcctaaactc acgcatgaat atgatgacag agttgaattt attcaagtta gcgaaaagga
    80341 tgcccctatt cccacaaatc aagacgaaaa aatgattgat aagggttata aaacacatgc
    80401 tctcgcaatg cgtttgcgag agttgggcgg cggtttcgct atgatggttg acgcagatga
    80461 tcttgtaagt tctcatttgg cggaatatgt gaattcccat ccccaggaaa atggatttta
    80521 tgtcaaaacg ggatacgtgt actttgttgg agacgattat atgaaggttc taccaaagtt
    80581 ctcatcagga agtgcgtgta tagtaaaata tgcagtggaa gacctgcctg aggcatatcc
    80641 ggaagttatg acagcaaatt gtgatgataa tccatggatc attagaaaac gacatggggg
    80701 cgttgttcca gcatgtgaag agcaagggcg tccattaaaa cctcttccat tcaaagcagc
    80761 agtatatgta ttaggaacag gggacaatca ctcgctttat ggtaaaacga caaaatatca
    80821 gacgaaaatg cgagagatac tggaaatgtt tatagcaaaa cgtaaaatta aaggaaaact
    80881 gaaagcagaa ttttctgttg attggttata aggtgagtat tatgaaggta ttgatgtgtg
    80941 aaagccaaat gccagagaat tggtattcgt ttgatctttc taaagaactt aatcaatatg
    81001 ttgatttaac attgctgtgt aagcgtgatg ttcctccttt agatcctaaa attaagagca
    81061 aacatatttt gtatcataaa agtacaaata aagtgttttc ggcacttgtt tttctgaaaa
    81121 gcttgttctt aatttggaag gaaataagat ttggtgatta tgatattgtt catttgcaat
    81181 tgatgaaaag tctcaaatat gaaggcggat tgtacgtttt atgtaagaga cactataaaa
    81241 aattaattat gacagttcat aatgtgttac cacatgaggt tcgtgaaaag gatcgccaag
    81301 agcactttaa tatatattca aattgtgatg gtttcattgt tcacaatcga gtaacagaac
    81361 agcatttata tgctgaattc ccagaattaa atgcaaagtc atatgtttgt ccacatgcta
    81421 tctatggaga tgttaaacat atatctagag cgtatgataa agataagttt gaatttatct
    81481 tgtttggaca aattcgtcca tataagggaa ttgatgtact gcttaatgca atcacagaac
    81541 ttccaatgga tgtaagaaag aaagttcatt ttaccatagc aggtcaacaa catcctaaac
    81601 aggatagtac tgactataaa aaaatgattg aagatttaaa ggtttcagat tgtgttctat
    81661 ataagccaga gagaatttct gatgaagaaa tggacagtat gttcagaaat gcagatgccg
    81721 caatatttcc atataaagaa atctatggaa gtggagcgct tcttatggca tacacttatt
    81781 gcttaccaac tattgttagt aatgtacctg cttttatgga agaaaccgag gaaggaaaga
    81841 tagggttact tttcgaaaat ttgaattcta agagcctagc agacacaatt gtaaagtatg
    81901 tgaaccttag cccagctgag aaaagcaaaa tagaggatga tatatatgag cttgtaaacc
    81961 gcaagtatac ctgggcggca tctgcaaaac tcacatacga ggcatacaaa caaattttga
    82021 atgagtagta aaacaattag aaaggaataa taattaacat gcagattagt aaatggaaaa
    82081 tcatacagtt tctaatggtc tttctaatga ttatctcatc aagcatattg aagcctttgg
    82141 gcggctggtt gggagaaacg atgaacgtta gacttgtgac agtcattcta gcaatagtat
    82201 gtttgttaat agcctcatta ccagcattta gaaaaaatag atatttaaat ttctgcactt
    82261 tactgatttg tgttgttata atagcagctt acatctactc agataagaca atatgggtta
    82321 aatctaggga aagtatgagt ttatactatg tattattggc gtatccattg ttctgtactt
    82381 taattaacca agcgtggaat ttggattcaa tgctaaatac gatatgtggt gtaacatttc
    82441 tttcatacat tcttagaaca tctatttcac ttattcagaa attttcagga gtacttctct
    82501 atgaaaacat atatcgtgag tgtgccccgg aaaattggta tcgtggaggc tttttaagaa
    82561 ttaatccacc atgttttaca attataataa taccgattgc tttgtatatg attgagcgac
    82621 aagttgtagc tagaagaaaa gtaaaatatt atttactgat tgctagcgca ctggcatatt
    82681 ctgctgttat tcatggttct agatctgtcc ttgtctatca aattattgta cttggttgct
    82741 atatcctttt taagaaaggt tcttcgaaaa gaaaaattgc aatgtgggtt ttagctgggt
    82801 tgcttgtagt catatttatt aattcgacca tgttctcaac atttgttgaa actttcacaa
    82861 acagtacagg tgaatatgca ggatctgctg aatctcgatt tgcttcagtt tggtttttta
    82921 ttcctaaatt tttgcaatcg cctttgttcg gtactggtat tcttactggt gaggaattta
    82981 cgtttatatt accaggagga gttagaggac acctatctga tattggattt ttctatacaa
    83041 ttactcaata tggtgtaggt atacttatat ttgatattct gtttattata aaagggttca
    83101 aaacagcatt acttgcatca aaagtgggta tgactggcta tcaatattta gcatttggaa
    83161 ttacattaaa tgtcttgcta acagggatta atatagactg gttttatccg atatttactc
    83221 cagctattcc tgtatgctta gcggttatag aatacatcta tcaagaatgt agaagtgtgg
    83281 ctaacataga ataggaaata ttaataggag tgcatctcat gaataagaaa gagattttat
    83341 ataacatatt gaagaagtac gaaaatttac catatgtgtt taggaacatg tttggtcata
    83401 atataagtaa actcgtatct tggaaattga ctgctgattg tgcaacagta gaagaaaatt
    83461 ctgaagttta tgcagcgttt caattgcgct caaaggacat gtctgatgtt ccagtgatgg
    83521 gtttagttaa ttgttgcaaa gatgtagcaa ttgtaatgca ggggccacta tgtttaaagg
    83581 atgatttctc ttataatact ctggcctact ataagaaatg ttatccaaat gctttaatta
    83641 ttctttcgac atggaaggac gaagatatta aggcattgaa aaggatagag gatcttggag
    83701 ttatagttat ttgtagtgag aaacctgaaa agcctggaca cttaaatatt aattatcaag
    83761 ttggaaacac tttgcctgga atagtcagag caaagcagct tggtgcaaag tatgtttgta
    83821 aaactagaac agatcaaaga atataccatc caaatgcaat ggcgtttttc tgcagtttac
    83881 ttgaacagta tcctgttaat aacgaagatg attggaaatt aatacaaaat cagcgattac
    83941 tgttactatc aatgccgtat ggagacatgt ttttcccgta ttgtttgtca gacttcctgt
    84001 attttggtga tgttgatgat atgataaacc tcttctcgat tcctagtgat gtgagagaaa
    84061 agggagctgt gtctagagga gtctcaagaa gggagatatc tgaaaataat ttagctccag
    84121 aagttcagct tcttcgaagc tatatttcac gaatgggggg caatgaggag tgctctatta
    84181 gggcatattg ggagtttgta aaaaaccatg ttattactat taacaaaaat caaattgatt
    84241 tgtactggca taaatatgta ggtcgttatt caaataatac tatttatggt acgtattata
    84301 ttgatgatag ccaagatgca cttcactgct ataattttga ttttataaat tggttgaatt
    84361 tgtacactgg aaaatacgaa tacaaagcag aatatgaaaa atatatggac tttgtttggg
    84421 aagagtaatt aacttttata gtaaaaagta ctttagggaa gggcatatta tgaaaacagt
    84481 aattatggcg ggcggacgag gaagccgaat ttcggaattg tttccggata ttcctaagcc
    84541 aatgattcct atagatggca ttccagtttt agaacgagaa ttgtgttcgc ttagagatca
    84601 aggctttaat gatattattt tgactgtttc ctatttgcat gaaaaaatag aggagcattt
    84661 cggagatggt tcaaaattag gaattaaaat caaatatttt attgaaaaaa ctccacttgg
    84721 caatgcaggt gcattgtatt ttttgttgga tgaactgggt gatgaaccgt ttttactttt
    84781 gaatgctgat gcagtatttg atgttgattt caggcagatg attagatttc atgttgagaa
    84841 taacgcagat gttactttgt ttacgcatcc taactcacat ccgtatgata gtggattaat
    84901 tatatccgat gacaataaga aagtgcttca atggttagcg aaggaagatc aaagacctga
    84961 atattataaa aaccgagtaa atgcaggtct tcatgttata aatccgaagg tgttgagaga
    85021 aaatccatta atggaaattg taaataaatc aatagttgaa ggtggggggc taccatacaa
    85081 aattgattta gatagaatga ttctgaaacc attggctaac acagggcgta tgtattgcta
    85141 cgattctcca gagtatgtta aagatatggg aacaccggac cgatatagag ctgtaattaa
    85201 ggactttcaa aagggaatta ttgttggtaa aaatctatcc aatccacaaa aagcaatctt
    85261 tttagataga gatggaacga ttaataaata cgttggtttt ttgagaaatg cagaagaatt
    85321 tgagttaatt gatggagtat cggaggcaat taaaattatt aattctctag gttttctgtg
    85381 tattgttata acaaatcagc ctgtaattgc aagaggtgaa gttactgttg atgagcttaa
    85441 tagtattcac aataagcttg aaacaatgct agggcatgct ggtggatttt taaacggtat
    85501 ttattactgt ccgcaccatc cccataaagg atatgcagga gaggttatag aattaaaaat
    85561 tgattgcaat tgtcgcaagc caaaacctgg actgttatta gaagcatcaa aggattttaa
    85621 cataaaccta agtcaatcat ttatggttgg agatagtgaa agtgatgtaa ttgctggaaa
    85681 aaatgctggt tgtaagacag tactgattac agaagacatc accttgcctg ctaattatgg
    85741 ccaagactat agtgtctgtt cattgttaga atttgttaaa aatcatttat gcactatgca
    85801 ataattggtt tagtgaaact tgtaaaagga gtaattagat gaaggtatta attccagcag
    85861 caaaagttgt tgaaaaagaa ttgcagagta taggaatgtt accagcaatt atctatccaa
    85921 ttaataaaaa aattgtcttt gattatttgt ataatcagta taatgcaatc tgcgattcat
    85981 tggatattat ctgttatgaa aaagcggata aggttcatag aagactgaaa aactatcatg
    86041 attccagaat tcatatatgt gaccttcctg aattagggga tctagggcaa accgtgtatt
    86101 ttgggttatc agatgaagat gagcctgttc taattaactt cgcagataca attgttatgg
    86161 agagtatgtc tgatatagaa atggatacat tctattattc tgaagatttt gtgtcgagta
    86221 cttggacata ctttaaaatt aatgaaggaa taattactga tatccacgat aaagaggaag
    86281 cttacgtatc cgataaacgt gaaaaattat ttattggcgt atttatgttt tcttcgtcaa
    86341 aacttctgaa gagttgctta agagaggcag ttgagagtcg gaaacagggg ggaataaact
    86401 cattttatcg ggctttacag ctatacagcg aacgttgtcc gctaaaggcc gtaagaacag
    86461 aaaagtggtt tgatattgga cacatcgata aatattacaa tacaaagttg gaagttaaag
    86521 cacgagagtt taatcatatc tctatagata aagatcgagg gatattgaag aaaactagcg
    86581 atgacaagga taaatttatt ggagaaataa agtggtattt aaaacttcct gctgatattg
    86641 aatatgtaag acctagaatt tttgattatt ctacatctta cgtagctccc tatgttgata
    86701 tggaatatta tgcatatcat acggttcatg agttatactt atatggtgat ttaaaatata
    86761 accagtggca tgatctgttt agccgtataa agtttatctg tagcgatttt aagcgataca
    86821 ccttaaaaga tgaaaagatt agagagtcat tggaagatat gtatttgcga aaaaccatgc
    86881 agagatttca aaaattgtct gaagataaaa gattttcagc tttttttgag aaacaaattc
    86941 aaattaatgg tcgtaagttt atttctttaa ccggtgtaat tgaacttcta aagaatctta
    87001 tcccaagtat gctctatgat gtaaatgaat tttgtattat acatggagat ttgtgttttg
    87061 ctaatatgat ggtagattcc aattgttcct ttgttaaggt tattgatcct aggggaaaat
    87121 ttggaaaata tgatatttat ggggacagca gatatgaatt agcaaaacta tttcatagtt
    87181 tagatggtaa atatgattat atcatcaagg atctatttac tgtagaatat gataccgagt
    87241 gtgcagtgat agattataca attaatacgc gtgtaagaga ttacgattta tttgaattgt
    87301 ttaagtcggt cttttcggac gagattaagg acgattttga taagattgta ttgatagaag
    87361 cgttgctatt tcttagtatg attccattac acggtgaaag ttttgatcac caaatagtga
    87421 tgcttgctac aggtatggaa cttcttgcaa gagtaagtaa taaagttata ctctaatata
    87481 aattgtgcct taatgaataa cggttacaat actgttttag caatgtaggc attaaggcac
    87541 gattatatta attaatacaa tagaagggaa gagaaaaatg tatagtgaat attcttttgt
    87601 gtttgatatc gatggcactt tgtgtccgat aaagaagaag gatgagcgat atgaggattt
    87661 ggtgccgtat gagaatatgg ttgaaaaact gagattctat aaggagaatg gtgctaaaat
    87721 tacattgttc acctcacgca atgttaattc ctataaagga aatttgggaa tgataaatgc
    87781 taatactgca aaagtgctct tggcgtggtt ggataaatgg gacattccgt atgatgaaat
    87841 cctgtatggt aagccttggc cggggcataa aggcttctat gtagatgatc gaacggttag
    87901 acctgatgag tttttgagat gctctgtaga tgaattaaat gaaatctgca acagttcaaa
    87961 aaaaagattc taaataagga ggttagttct atggaaaaaa agatgacagt tatcattacg
    88021 atggcaggtt tgggaagtcg ttttcgaaaa attggttatg cggttcctaa atatatgatt
    88081 aatgccaaag gaagaacttt atttgattgg gccatggata gtttggttgg atacaataaa
    88141 aatgttgata agtatatttt tgttgttcgt caggaagaca acgcttcagc ttttataaag
    88201 gagcattgtg cttactttgg tattgaaaac gtagaagtga ttgaaataga ctatatgaca
    88261 gatggtcaag caactacatg tatgttggct attccttatt gcaatcaaga caatgagatt
    88321 atggtttaca atattgatac atacgtggaa tcaaatgaga tgaaatacga agatatttgt
    88381 ggagatggac atattccatg ctttcatgct gatggcgatc attggagttt tgcaaagctt
    88441 aacgatcgaa atgaagtaat agaagtccgc gaaaaagttc gcatttctga taattgtact
    88501 cttggtgcgt attatttctc atcggcaaag ctgtataaag ctttatatga agagtattat
    88561 gcagactcct ctaatatgga gaagaatgaa aaatatatag caccattata taactttatg
    88621 ataaaaaaag ggttaaaggt gactattagc atagtagatg cgaaaaaggt tcatgtgctt
    88681 ggtactccag aagagctcaa tgtgtttttg aatcaagaat aaatagtgtt taggagaaaa
    88741 tagcagtgac cagtaataat ctaattgtaa aaacattaag cagatataag aatactccac
    88801 ttcctgttaa ggctgcatta tggtttatgg tatgtggttt tttacagaga ggggtttcaa
    88861 tgattacaac cccaattttt actagagttt tgtctacaga acaatacggt ttatacagca
    88921 cttatctatc gtgggaatcc ttattgctca tgattacatc gcttagtttg ttcaaagcca
    88981 tgatgaatct ttatgtaaaa tatgataatc aagaacatat tctttcgaca gtatccggat
    89041 tgactttagt actgactatt ttttggtttg ttattgctat ttttattaag gatatattgg
    89101 cagaaatttt aggattgaca ccagttctag ttgtctgtat gttctcatat tttatatttc
    89161 aggcagaaat acaatgttgg tctttacaca aacgatatat atatgattat aagactttat
    89221 tagtagctac ccttgcagtt actgttggct catctgtagt tggctgttta gcggttctgt
    89281 ttatatctcc taccgctgag gcgagagcct taatgaatgt cctagtgact ggtgttgttg
    89341 gcgtggctgt gtatatatcg gtatttagaa gatgtaagga ttttaaggat aatgaagtgt
    89401 ggaatttcgc agtagtattt tgtgtatcgt tgatgccaca ctatttatct gaatttattc
    89461 tgaatggttc tgataaaatt atgataaatt acttatgtgg ttcgtcagat gttgctattt
    89521 acagcgtggc atattctatt ggtgctctaa ttaaccttgt tacaaattct gtcaattcca
    89581 cttttgcacc ttatcagtat caaaaaatca agtcaggaga atatgattta ctatcaaaaa
    89641 gagctaatga agttcttggt ttggtagcgg ctatgctttt tgcattaatg ctatttagta
    89701 aagaaattgt gttgatattt ggtgggcaga aatatatcga cagtgttaat ttaattgtac
    89761 ctatatgtat cggtatttat ttcaactata tgtttcagct gtttgctaga gtacaggaat
    89821 actttgaaaa gaaactcact gtcgttattc catcggtttt gtgtgcaatt atgaatattg
    89881 tactaaatta tgtttttata aagttatatg ggtatcaggc ggtagcatac acaactgtat
    89941 tctcatatat gtttttctgc gtcatccatt atatttttta tagaaaagtt tgcaaagaac
    90001 tattggatgg aaaaaacata tatgatgtca gaggaatact tttgatttct gcaatcacga
    90061 tagctggtgg catcctaatt tcaataatta ataaatttga tttcataaaa tatttaatta
    90121 tagcaattgc ttgtgtcgct gcaattatat ttagaaaacg attgctactt ttagtaagga
    90181 gaattatgag caaataaaca tttgctcata gagagataga gaatgataga tattatttct
    90241 gctataacgg taaatgtatt aacagcatta tatcagccgt tcttatttgc cgtattaaca
    90301 gcgtatctgt ttatgtactg gtatctattt atagttgata actctcgaga tcctaaaaac
    90361 ttaaaagatt ctgtattaat ctggttgaga tattttaaag catctagtaa atttcgcaga
    90421 ctcttttttc ttgcgtttta ttttactttg atattattta aaaccttgct taatcgaaat
    90481 atgtggatga acccgctttc aaacgttatt ggacagtggt cattatatat tatagataat
    90541 gctacaggag agagcagatt gacaacagaa tgcgtggaga acacgatgct gtttatccct
    90601 ttttgcatgt tattgttatg gtggcgtgac attaaatgtg atattatacg caccatctat
    90661 gttgggatta agtatgtgtt tttattctca ttaagtatag aatttacaca actgtttttt
    90721 aggcttggta catttcaact atcggatttg ttttataaca ccctaggggg cttaattggt
    90781 gctttgctat attggctatt ctaccgatta aataagtata tagacttaaa atagaactgc
    90841 tttgaatttt aatatggtta atatttttct gcagacagga ttgtgtaatc tcatcaagaa
    90901 tactttacat gccatctatt tttgctaaaa cattgttgtt atatcttcaa gaggtaggga
    90961 attttattgc tttaaatgag gtcgagcgaa ttaaaaaatt ggtaaaatat ataacatggt
    91021 ggtagttatg gtaaaagtgt ttgatataat taagttttcg gatttcagag gatatactgc
    91081 tgttgcattg gataatgaga ctcttttctc gattggtatt gagtttcaca ttaagcaaat
    91141 aaatcaagga tttagtcata ataaatatac tatgcgaggg ctacattatc agagtggaga
    91201 gcatgctcaa ggaaagatag tgatgtgcct acatggtgaa attatgaata tcgcacttga
    91261 tattcgacaa ggatcttcca cttatggatg tgtgtatgtc aacacattga gtgaaagcaa
    91321 tggtaaatgt gcgtatatcc cacgtggata tgctcatggc tatcttacta tggaggacaa
    91381 tacgctagtt cagtggtgcg tagataatga tttcaattct aatgctgcca aatgtataag
    91441 ttatgaaagc gtagatattg aatggccggt cagtcggcag tgtttcgtta taagtgagaa
    91501 agacaaaaat gtggaaagat taagcttttg attaaaatga gctgtggaga aaagaaggat
    91561 actggaaaat aagaagaaca ataaccgagc aaatgctcgg ttatttctat gaggaataca
    91621 taaaactaaa tttagatata gatatacccg tggtgctagt taattttaat atcctaaaga
    91681 atatctcaaa gttccttcaa aacgtcgttg tgctcgactt aatagaacac tgtaagccag
    91741 taatatgctt tcaaatctac tctctagccc tttaacagaa cgtgagttga aattttgaca
    91801 acctaatttt tctaaggaag aaaaaacagt ctcaactgtt ttt
//
LOCUS       DACDXO010000004        88704 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E23605_2017
            SAMN12639989-rid9881463.denovo.004, whole genome shotgun sequence.
ACCESSION   DACDXO010000004 DACDXO010000000
VERSION     DACDXO010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639989
            Sequence Read Archive: SRR10023389
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 88704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 88704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741914.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:42:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,972
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 33 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 17 of 73
            Pseudo Genes (multiple problems)  :: 10 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..88704
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639989-rid9881463.denovo.004"
                     /isolate="E23605_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(57..173)
                     /locus_tag="IWM35_000358"
     CDS             complement(57..173)
                     /locus_tag="IWM35_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HAP9918243.1"
                     /translation="MILKYPENICMLCMPMVMHKNPSDKSIYHWDFYALLGF"
     gene            complement(418..495)
                     /locus_tag="IWM35_000359"
                     /pseudo
     CDS             complement(418..495)
                     /locus_tag="IWM35_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646598.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            complement(649..1284)
                     /locus_tag="IWM35_000360"
     CDS             complement(649..1284)
                     /locus_tag="IWM35_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9918244.1"
                     /translation="MEGYAMKISHLGNNIQTIRKFRGMKQQELADKIGINMQSLSKIE
                     RGLNYPAYETLEKIMEVLDVTPNELLSGEWKYVNQSEKEVCQFLRAEERLNAELKHGH
                     YDNFFDSEEEWLEYELEKLREYITDYINGKSIEASDLYPIKEFIQHLKFQKLLDRYDD
                     LYSMDMFGESIEGHKYKTPYQVVKMINPNSKEDMELLREVLRNNNFDDEDE"
     gene            1796..2938
                     /locus_tag="IWM35_000361"
     CDS             1796..2938
                     /locus_tag="IWM35_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAP9918245.1"
                     /translation="MNNLEFALEMKNKRLASGLSQGELASLTHVSRYSINRFENGKAN
                     ASKETQNIILRCLNYYICDKPFYLLVDYLSVRFPTTDALEIIRKVLGMKADYFIHYDY
                     GYYGYKEHYAYGEIKVMASDDEHMGVFLELKGAGSRNMEYVLQAQNRDWYSFLNRCLD
                     CGGVIRRFDLAINDMCGLLDIPVLSEKYKNGGADCRCKNYENVQGGKLSGKNRNLAST
                     LYIGSKASTKYFCLYEKQKEQATKKKHTDIINRFEIRLRDKKAVQAVEELLLTYNPHG
                     LVFYLITDFVQFPDYPLWEIFISHDSLPFEMNPVPVNMERTLQWLERQVMPSIVMIEE
                     IDRLTGSNYMKMIDECTHLSEKQEMLVEQMCTDIADVIESEGVFYE"
     gene            2931..3137
                     /locus_tag="IWM35_000362"
     CDS             2931..3137
                     /locus_tag="IWM35_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HAP9918246.1"
                     /translation="MSNAQDIPVWEKYTLTIEEASKYFRIGENKLRRLAEENKDAGWL
                     IMNGNRIQIKRRQFEQVIDKLDAI"
     gene            3216..4409
                     /locus_tag="IWM35_000363"
     CDS             3216..4409
                     /locus_tag="IWM35_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP9918247.1"
                     /translation="MKEKRRDNKGRILHTGESQRTDGKYLYKYVDAFGNTKYVYAWRL
                     TPTDPTPKGKREKTSLRELEQQIRRDIEDGIDSTGKKMTLCQLYAKQNEQRANVKKST
                     MKQREQLMRLLKEDKLGARSIDTIKPSDAKEWALRMKEKGFSYNTINNHKRSLKASFY
                     IAIQDDCVRKNPFDFKLSEVLENDTKEKVALTEEQEQALLSFIKTDNVYHKYYDDVLI
                     LLKTGLRISELCGLTVADIDFKNEVVIIDHQLLKSKEQGYYIETPKTKNGTRQVPLSR
                     ETIQAFQRVMKKRPKAEPFVIDGRSNFLFVNHKGKPKVAIDYNALFVRMVKKYNKQHK
                     DNPLPHITPHTLRHTFCTRLASKNMNPKDLQYIMGHSNISITMNWYAHASIDTAKSEV
                     QRLIA"
     gene            complement(4416..4574)
                     /locus_tag="IWM35_000364"
                     /pseudo
     CDS             complement(4416..4574)
                     /locus_tag="IWM35_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008788611.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-binding domain-containing protein"
     gene            4674..5366
                     /locus_tag="IWM35_000365"
     CDS             4674..5366
                     /locus_tag="IWM35_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HAP9918248.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQALTDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSLKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
     gene            5574..5864
                     /locus_tag="IWM35_000366"
     CDS             5574..5864
                     /locus_tag="IWM35_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9918249.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            6578..7948
                     /locus_tag="IWM35_000367"
     CDS             6578..7948
                     /locus_tag="IWM35_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9918250.1"
                     /translation="MNKHNRKITNNKELILLLIGIWGIFLLFNMYMPTMRADDVVYAS
                     RLDELGYLGASIEHYKTWSSRIIIELFLMFFSKHFMLWKLLNSTIMLGIVVLLCKYVF
                     EKINVKNLLLVCSIYCLIPLTVMGETGWRATTLNYQWPVAFSLLTFYPFFQLLRGEEI
                     NRKIYWVSIPLLIFLTNQEQVNACFFVLTSIVSLYLIVNGRYNYKLSVFSIISLAELI
                     FSLTTPGNALRAAHEINKWFPEYKNFNFLNKLDLGISSFGKPFFLDMNILFLLLFFLI
                     FLLTYRKCQNYYVRILTALPFFLNLIIYFGNTMGQSFTYVNGNKRAMIWSSSNLNNLF
                     TELGTKLSLFYPGTWIATLVVLALLLCLIVGIYLSFDNKKTSIFLVILMIMGFCSRLI
                     MGFSPTVWASGMRTYYILYVVIAILVLMAVKELMKSMSVQKNEFMQFGLTVLGICTFI
                     ITVINR"
     gene            complement(8188..8271)
                     /locus_tag="IWM35_000368"
     tRNA            complement(8188..8271)
                     /locus_tag="IWM35_000368"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8235..8237),aa:Leu,seq:gag)
     gene            8654..9871
                     /locus_tag="IWM35_000369"
     CDS             8654..9871
                     /locus_tag="IWM35_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAP9918251.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            10055..10891
                     /locus_tag="IWM35_000370"
     CDS             10055..10891
                     /locus_tag="IWM35_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9918252.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            10967..12115
                     /gene="nagA"
                     /locus_tag="IWM35_000371"
     CDS             10967..12115
                     /gene="nagA"
                     /locus_tag="IWM35_000371"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HAP9918253.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            12335..13393
                     /locus_tag="IWM35_000372"
     CDS             12335..13393
                     /locus_tag="IWM35_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014372913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HAP9918254.1"
                     /translation="MNLFDIFRSTLTDMSIVSAITSTVFIILLGFVCRKKGIFSESVG
                     KILSKVVLTVALPALAFRAFMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYK
                     GDKQTTLRVLTIFGSTTFFGTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGL
                     KMEAKNIKAMFVNPIIIATFAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPW
                     LFQPMDYLAGLSSPLAWLAIGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILA
                     VLTATNILPVNFEALATIVIMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVL
                     TPVWIVVLQMIHGIGLFS"
     gene            13412..14431
                     /locus_tag="IWM35_000373"
     CDS             13412..14431
                     /locus_tag="IWM35_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate dehydrogenase"
                     /protein_id="HAP9918255.1"
                     /translation="MRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIVSAKDH
                     DAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVPSYSPN
                     AIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRIGLTEA
                     VLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQMINEG
                     FLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSFKPWET
                     IPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNEVSLPK
                     ARQLR"
     gene            complement(14533..15249)
                     /locus_tag="IWM35_000374"
     CDS             complement(14533..15249)
                     /locus_tag="IWM35_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAP9918256.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     QPLTCYELTKNGRKKLTDYC"
     gene            complement(15218..16003)
                     /locus_tag="IWM35_000375"
     CDS             complement(15218..16003)
                     /locus_tag="IWM35_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAP9918257.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDGGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            complement(15994..16743)
                     /locus_tag="IWM35_000376"
     CDS             complement(15994..16743)
                     /locus_tag="IWM35_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HAP9918258.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            17041..17565
                     /locus_tag="IWM35_000377"
     CDS             17041..17565
                     /locus_tag="IWM35_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9918259.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            17581..19080
                     /locus_tag="IWM35_000378"
     CDS             17581..19080
                     /locus_tag="IWM35_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HAP9918260.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(19168..19770)
                     /locus_tag="IWM35_000379"
     CDS             complement(19168..19770)
                     /locus_tag="IWM35_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAP9918261.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            19874..20830
                     /locus_tag="IWM35_000380"
     CDS             19874..20830
                     /locus_tag="IWM35_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HAP9918262.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            complement(21135..22091)
                     /locus_tag="IWM35_000381"
     CDS             complement(21135..22091)
                     /locus_tag="IWM35_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP9918263.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            22265..22825
                     /locus_tag="IWM35_000382"
     CDS             22265..22825
                     /locus_tag="IWM35_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9918264.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            22818..23642
                     /locus_tag="IWM35_000383"
     CDS             22818..23642
                     /locus_tag="IWM35_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HAP9918265.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            23652..24269
                     /locus_tag="IWM35_000384"
     CDS             23652..24269
                     /locus_tag="IWM35_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HAP9918266.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            24287..24970
                     /locus_tag="IWM35_000385"
     CDS             24287..24970
                     /locus_tag="IWM35_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HAP9918267.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            25653..26888
                     /locus_tag="IWM35_000386"
                     /pseudo
     CDS             25653..26888
                     /locus_tag="IWM35_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
     gene            26896..27783
                     /locus_tag="IWM35_000387"
     CDS             26896..27783
                     /locus_tag="IWM35_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP9918268.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            27800..28636
                     /locus_tag="IWM35_000388"
     CDS             27800..28636
                     /locus_tag="IWM35_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP9918269.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            28639..29262
                     /locus_tag="IWM35_000389"
     CDS             28639..29262
                     /locus_tag="IWM35_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9918270.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQPDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            29259..30551
                     /locus_tag="IWM35_000390"
     CDS             29259..30551
                     /locus_tag="IWM35_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP9918271.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     regulatory      30910..31007
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            31123..31704
                     /locus_tag="IWM35_000391"
     CDS             31123..31704
                     /locus_tag="IWM35_000391"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HAP9918272.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     gene            31707..33014
                     /locus_tag="IWM35_000392"
     CDS             31707..33014
                     /locus_tag="IWM35_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HAP9918273.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            33307..33795
                     /gene="purE"
                     /locus_tag="IWM35_000393"
     CDS             33307..33795
                     /gene="purE"
                     /locus_tag="IWM35_000393"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HAP9918274.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            33779..34912
                     /gene="purK"
                     /locus_tag="IWM35_000394"
     CDS             33779..34912
                     /gene="purK"
                     /locus_tag="IWM35_000394"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HAP9918275.1"
                     /translation="MINLVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVS
                     DCPAAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTD
                     LLAITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKG
                     QYVLKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHN
                     NILHETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRP
                     HNSGHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENK
                     PDWQFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            34925..36220
                     /gene="purB"
                     /locus_tag="IWM35_000395"
     CDS             34925..36220
                     /gene="purB"
                     /locus_tag="IWM35_000395"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HAP9918276.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(36400..36777)
                     /locus_tag="IWM35_000396"
     CDS             complement(36400..36777)
                     /locus_tag="IWM35_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9918277.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
     gene            37033..37761
                     /locus_tag="IWM35_000397"
     CDS             37033..37761
                     /locus_tag="IWM35_000397"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HAP9918278.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            37761..38015
                     /gene="purS"
                     /locus_tag="IWM35_000398"
     CDS             37761..38015
                     /gene="purS"
                     /locus_tag="IWM35_000398"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010758866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HAP9918279.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            38017..38688
                     /gene="purQ"
                     /locus_tag="IWM35_000399"
     CDS             38017..38688
                     /gene="purQ"
                     /locus_tag="IWM35_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HAP9918280.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            38689..40911
                     /gene="purL"
                     /locus_tag="IWM35_000400"
     CDS             38689..40911
                     /gene="purL"
                     /locus_tag="IWM35_000400"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HAP9918281.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            40896..42335
                     /locus_tag="IWM35_000401"
     CDS             40896..42335
                     /locus_tag="IWM35_000401"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010768528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HAP9918282.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            42367..43410
                     /locus_tag="IWM35_000402"
     CDS             42367..43410
                     /locus_tag="IWM35_000402"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HAP9918283.1"
                     /translation="MANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGFGGC
                     FDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEPLYF
                     LDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFAVGI
                     AEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKKTLG
                     EELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSWPIL
                     PIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVEESKVKEIVHEIEQAGEKVYEIGKVIA
                     KEKHRVVFIGEER"
     gene            43407..43985
                     /gene="purN"
                     /locus_tag="IWM35_000403"
     CDS             43407..43985
                     /gene="purN"
                     /locus_tag="IWM35_000403"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HAP9918284.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            43982..45517
                     /gene="purH"
                     /locus_tag="IWM35_000404"
     CDS             43982..45517
                     /gene="purH"
                     /locus_tag="IWM35_000404"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HAP9918285.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALAFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            45533..46807
                     /gene="purD"
                     /locus_tag="IWM35_000405"
     CDS             45533..46807
                     /gene="purD"
                     /locus_tag="IWM35_000405"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HAP9918286.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            46929..47843
                     /locus_tag="IWM35_000406"
     CDS             46929..47843
                     /locus_tag="IWM35_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HAP9918287.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(47976..49541)
                     /locus_tag="IWM35_000407"
     CDS             complement(47976..49541)
                     /locus_tag="IWM35_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAP9918288.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(49534..50985)
                     /locus_tag="IWM35_000408"
     CDS             complement(49534..50985)
                     /locus_tag="IWM35_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP9918289.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIREIKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITA
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(50999..52015)
                     /locus_tag="IWM35_000409"
     CDS             complement(50999..52015)
                     /locus_tag="IWM35_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP9918290.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            52235..53323
                     /locus_tag="IWM35_000410"
     CDS             52235..53323
                     /locus_tag="IWM35_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAP9918291.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            53377..54903
                     /locus_tag="IWM35_000411"
     CDS             53377..54903
                     /locus_tag="IWM35_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HAP9918292.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            54905..56110
                     /locus_tag="IWM35_000412"
     CDS             54905..56110
                     /locus_tag="IWM35_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP9918293.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            56367..57344
                     /gene="guaC"
                     /locus_tag="IWM35_000413"
     CDS             56367..57344
                     /gene="guaC"
                     /locus_tag="IWM35_000413"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HAP9918294.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            57608..59032
                     /locus_tag="IWM35_000414"
     CDS             57608..59032
                     /locus_tag="IWM35_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HAP9918295.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
     gene            59493..60602
                     /gene="pdhA"
                     /locus_tag="IWM35_000415"
     CDS             59493..60602
                     /gene="pdhA"
                     /locus_tag="IWM35_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HAP9918296.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            60605..61582
                     /locus_tag="IWM35_000416"
     CDS             60605..61582
                     /locus_tag="IWM35_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HAP9918297.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRKLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
     gene            61607..63250
                     /locus_tag="IWM35_000417"
     CDS             61607..63250
                     /locus_tag="IWM35_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005236379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HAP9918298.1"
                     /translation="MAYQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHEDNEGDSGVAAESQTPAKPAA
                     EPTVDTESAGSSSEGVFQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSAEAPKEKVETS
                     GSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKEDIENFLAGGPS
                     SAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPTRKAIAKAMVN
                     SKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALTATVKKYPVLN
                     ASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEINEKAKLAHDG
                     KLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIVNAEGEIVVGR
                     VMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            63258..64664
                     /gene="lpdA"
                     /locus_tag="IWM35_000418"
     CDS             63258..64664
                     /gene="lpdA"
                     /locus_tag="IWM35_000418"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HAP9918299.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            64826..65101
                     /locus_tag="IWM35_000419"
     CDS             64826..65101
                     /locus_tag="IWM35_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HAP9918300.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            65107..65874
                     /locus_tag="IWM35_000420"
     CDS             65107..65874
                     /locus_tag="IWM35_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HAP9918301.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            66042..67871
                     /gene="typA"
                     /locus_tag="IWM35_000421"
     CDS             66042..67871
                     /gene="typA"
                     /locus_tag="IWM35_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HAP9918302.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            68132..69904
                     /gene="recQ"
                     /locus_tag="IWM35_000422"
     CDS             68132..69904
                     /gene="recQ"
                     /locus_tag="IWM35_000422"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HAP9918303.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            70018..70320
                     /locus_tag="IWM35_000423"
     CDS             70018..70320
                     /locus_tag="IWM35_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HAP9918304.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            70323..71486
                     /locus_tag="IWM35_000424"
     CDS             70323..71486
                     /locus_tag="IWM35_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAP9918305.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            71512..74940
                     /locus_tag="IWM35_000425"
     CDS             71512..74940
                     /locus_tag="IWM35_000425"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HAP9918306.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            74961..76085
                     /locus_tag="IWM35_000426"
     CDS             74961..76085
                     /locus_tag="IWM35_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9918307.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            76075..76512
                     /locus_tag="IWM35_000427"
     CDS             76075..76512
                     /locus_tag="IWM35_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HAP9918308.1"
                     /translation="MIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESAKVC
                     QLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQLQS
                     LLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            76496..76834
                     /locus_tag="IWM35_000428"
     CDS             76496..76834
                     /locus_tag="IWM35_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HAP9918309.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            76953..77513
                     /gene="rsmD"
                     /locus_tag="IWM35_000429"
     CDS             76953..77513
                     /gene="rsmD"
                     /locus_tag="IWM35_000429"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HAP9918310.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            77510..78001
                     /gene="coaD"
                     /locus_tag="IWM35_000430"
     CDS             77510..78001
                     /gene="coaD"
                     /locus_tag="IWM35_000430"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HAP9918311.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            77991..79034
                     /locus_tag="IWM35_000431"
     CDS             77991..79034
                     /locus_tag="IWM35_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HAP9918312.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
     gene            79193..79921
                     /locus_tag="IWM35_000432"
     CDS             79193..79921
                     /locus_tag="IWM35_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComEA"
                     /protein_id="HAP9918313.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
     gene            80037..80282
                     /locus_tag="IWM35_000433"
     CDS             80037..80282
                     /locus_tag="IWM35_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAP9918314.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            80279..80497
                     /locus_tag="IWM35_000434"
     CDS             80279..80497
                     /locus_tag="IWM35_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HAP9918315.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            80633..81172
                     /locus_tag="IWM35_000435"
     CDS             80633..81172
                     /locus_tag="IWM35_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HAP9918316.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            81165..83438
                     /locus_tag="IWM35_000436"
     CDS             81165..83438
                     /locus_tag="IWM35_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HAP9918317.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            83476..84522
                     /gene="holA"
                     /locus_tag="IWM35_000437"
     CDS             83476..84522
                     /gene="holA"
                     /locus_tag="IWM35_000437"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HAP9918318.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(84611..84865)
                     /locus_tag="IWM35_000438"
     CDS             complement(84611..84865)
                     /locus_tag="IWM35_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HAP9918319.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(85265..85534)
                     /locus_tag="IWM35_000439"
     CDS             complement(85265..85534)
                     /locus_tag="IWM35_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9918320.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            complement(86004..86459)
                     /locus_tag="IWM35_000440"
     CDS             complement(86004..86459)
                     /locus_tag="IWM35_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HAP9918321.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            complement(86597..88525)
                     /locus_tag="IWM35_000441"
     CDS             complement(86597..88525)
                     /locus_tag="IWM35_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HAP9918322.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
BASE COUNT        28940 a        15269 c        19018 g        25477 t
ORIGIN      
        1 gcatcaacat gagctaaaac tccaatatta ataattttca tgtgattttc ctccattcaa
       61 aagcccaaaa gggcataaaa atcccagtga taaatactct tatcactggg atttttatgc
      121 ataaccatag gcatacaaag catacagata ttctccggat actttagaat cacatgataa
      181 aggtattctt aaactgggta caaaaaacta agccctccta aaaaaggaca tccaattatt
      241 tgttcccact atcaaattga cagtttattt aagaatacct tgccgcatat ttattaactc
      301 cttttaaata gatacttaaa taatagcacg taagagcata tttgtaaagg aatctccaat
      361 tttttatcaa agagagtacg tgattacaaa atagctgtaa taatgtacca atatttgtta
      421 ttctataatc ttccaattac tcccgttctt ttcaagtacc aaatcaaatt gagatacctg
      481 cgttgccttt gcctgattta ccacaaatca gattttatcc ctttcttcct cttgattgcc
      541 cacaacagaa aagaaaaagc cctgtcaaga gctttgccac cattggtggt gctttgcact
      601 cttgatcggg ctttttgttt gctgtatctt ctcgcaagag gaaaaaagtt actcgtcctc
      661 atcatcaaaa ttgttattcc ttaatacttc tcgtaacagt tccatatctt cttttgagtt
      721 cgggtttatc attttcacga cttgataagg tgtcttatac ttatgtccct ctatgctttc
      781 cccaaacata tccatactgt ataaatcatc atagcggtct aataattttt gaaacttcaa
      841 atgctggata aattctttga ttgggtaaag gtcggacgct tcaatgcttt tcccgttgat
      901 atagtctgta atatattccc gtaacttttc taactcatat tccagccatt cttcctcgct
      961 gtcaaagaag ttatcatagt gtccatgttt cagttctgca tttagacgtt cttcggctct
     1021 taaaaactga cagacttcct tttcagattg attgacatac ttccattctc ccgacaacaa
     1081 ttcattgggc gtaacgtcca gcacttccat tatcttttcc agcgtttcat aggcaggata
     1141 gttcaaccct ctttcaatct tggaaaggct ctgcatattg ataccgattt tgtccgcaag
     1201 ttcctgttgt ttcattcccc taaattttct tatggtctgt atattatttc ctaaatggct
     1261 tattttcatt gcataaccct ccatatctcg ttatatcttc gctattataa gtctatcatg
     1321 cttaatatga tttgtaaagg caaaaaaaga tattgacaaa taagcatata agaattacta
     1381 tgttcttgta atcgaataag cacaatagaa tgaaatataa aaacattcaa aaagaaatac
     1441 ctatctgatt aaatccgtag atatgaatat cggttgaaat ggaacttgtt agaaaaggca
     1501 aaacagaact tgctgacaaa gtatttctga cacggtggcg gaagcctctc aggcttaccg
     1561 ccatgacacc ttgtgtcaag gggaagaaat gctttgtcgg ctggcgagcc tgcgagcctt
     1621 gtctgtgccc ccgttatcta acaggggggc actatttcac aaaagacaag tcaaaaacca
     1681 tacaacccca aagctgtaag gagtaatgag gagttttaag aagatagtaa tttcacattc
     1741 ttttgaatag tggtttacag gtatttcaag gctattgtgg gattggagga atacaatgaa
     1801 caatttagaa tttgcacttg aaatgaagaa taaacgtctt gcaagcggtc tttcacaagg
     1861 agaacttgca agtcttaccc atgtatcaag atatagtatc aaccgctttg agaacggaaa
     1921 agccaacgcc agcaaggaaa cacaaaatat tatcctgcgt tgtctgaatt actatatctg
     1981 cgataagcct ttttatctgc tcgttgatta tctgtctgtc cgttttccaa cgactgacgc
     2041 attggaaatt atccgaaagg tgcttggcat gaaagcagat tattttatcc attatgacta
     2101 tggatattat ggctataaag agcattacgc ctacggagaa atcaaggtta tggcttctga
     2161 tgatgaacac atgggcgtat ttttggaatt aaaaggggca ggctcacgca atatggaata
     2221 tgttttacag gcacagaaca gggattggta ttcattcttg aaccgctgtc ttgactgtgg
     2281 cggtgttatc cgacgttttg acttggcaat caacgatatg tgcggtctgc tggatattcc
     2341 cgttttatct gaaaaataca aaaatggcgg tgcggattgt cgctgtaaaa actatgaaaa
     2401 tgtgcaaggc ggtaaactaa gcggaaaaaa ccgcaattta gcaagcaccc tttatatcgg
     2461 ctcaaaagca agcacaaaat atttctgcct gtatgaaaaa caaaaggaac aggcaactaa
     2521 aaagaaacat acggatatta tcaaccgttt tgaaattcgt ctgcgtgaca aaaaggcagt
     2581 acaggcagtt gaggaactgt tattgacgta taaccctcat gggctggtgt tttaccttat
     2641 tactgatttt gtgcaatttc ccgactatcc gctgtgggag atattcattt cccatgatag
     2701 tttacctttt gaaatgaacc ccgtacctgt caatatggaa cgcactctgc aatggctgga
     2761 aagacaggtc atgccgtcaa tcgtgatgat agaggaaatc gacaggctga caggctcaaa
     2821 ctacatgaaa atgattgatg aatgtaccca cctttctgaa aaacaggaaa tgcttgtgga
     2881 acaaatgtgt acggacatag cagacgtaat tgaaagtgag ggggtgtttt atgagtaatg
     2941 cacaggatat tcccgtatgg gaaaaataca cccttaccat tgaagaagcg tcaaagtatt
     3001 tccgtatcgg agaaaacaag ttaagacgct tggcagagga aaacaaggac gctggctggc
     3061 tcattatgaa tggcaaccgc atacagatta aacgacgaca gtttgaacag gttattgata
     3121 aattggacgc aatctaatgc aaatgagcct tgtatgtgtt atgatgaaca caagtcatat
     3181 caaggctctt tccaacaagg aaaggagcag acaccatgaa agaaaaaaga cgggataaca
     3241 aaggacgtat cctgcatact ggagagagcc aacgaacaga cggaaaatac ttgtataaat
     3301 atgtagacgc atttggaaat acaaaatatg tgtatgcttg gagattgaca cccacagacc
     3361 cgacaccaaa gggaaaacgg gaaaaaacct cacttcgtga actggaacag cagataagac
     3421 gggatattga ggacggtatc gacagcacag gcaagaaaat gacgctttgc caactctacg
     3481 ccaaacagaa cgaacagagg gcaaacgtga agaaaagcac aatgaaacaa cgggaacagc
     3541 tcatgcggtt attgaaagag gacaagttgg gtgctaggag cattgatacg ataaaaccct
     3601 ctgacgctaa ggaatgggcg ttacgcatga aagagaaagg cttttcctac aatactatta
     3661 acaaccataa acgctcgtta aaagcgtcat tctatatcgc catacaagac gattgtgtaa
     3721 ggaagaaccc ttttgatttc aagttaagtg aagtcctaga aaatgatacc aaagagaaag
     3781 tggcattgac agaggaacaa gaacaagccc tactgtcatt tattaagacg gacaacgtgt
     3841 atcataagta ttatgatgat gtgctgatac tgttaaagac tggacttcgt atctcggaac
     3901 tgtgcggact gacagtagcc gatattgatt tcaagaatga agttgtgatt atcgaccacc
     3961 agttattaaa gagcaaggaa cagggctatt atattgaaac gcctaaaaca aagaacggaa
     4021 caaggcaagt gccattaagc agagaaacaa tacaggcatt tcaacgggtt atgaaaaaac
     4081 gcccaaaggc agaaccattt gtgatagacg gacggagcaa ttttctgttt gtcaatcata
     4141 aaggcaagcc caaagttgcg attgattaca atgccttatt tgtccgtatg gtaaagaaat
     4201 acaacaagca acacaaggac aatcccttgc cacatatcac accgcatacg ctacgccata
     4261 cgttctgcac aaggctggca agcaagaaca tgaacccgaa agatttacag tatatcatgg
     4321 gacattcaaa catcagtatc acaatgaact ggtatgctca tgcgtccata gataccgcaa
     4381 aatcagaggt tcagcgtcta atcgcataga agtatttacc acgattttaa ccacgtttga
     4441 tagcgaaaat ataaaaagat agacctagat atgtgaggtt taccacaaaa gcaaaatgcc
     4501 cgtagagccg ataaaataag gctttgcgga catttgagaa gatataaaaa gatagtcaaa
     4561 aaggcatata taatttatat taaaattaca catgtaattt aaaaacgctt gacattgtgt
     4621 ttgagatcac ttacttttag catgtaaaag tattggatta aggagctatg caaatgaaaa
     4681 agaaatttat tactggtggg attgtcgttg catttctatg tctaattggc tacacaggca
     4741 cgcggtacgc aaatgaaatt attttgtggg gtggagagac agatattgat acaatcaatg
     4801 agaatctgac acagcttgat catacattga cagataaaga gcaagcgctt acagatctta
     4861 ccgctcgact agcacaaaaa gaacagacat tgcaagattc aaaggcagat gtccaagaat
     4921 accaaacaaa agtaaatgaa ttagaaaacc aaaaaaatca atcggcagca gaaaaacaaa
     4981 acttagaaag tcaattagtc agcaaggatg cacaactaca aagcaaacaa aatgaaatca
     5041 atgtcaaact agatgagatc aaccaaaaaa atgatgaaat caatcatctg aatgcagatt
     5101 ggacagcaag actacaagaa gcacaagcaa aactttcaga attacaaaat actgtaaatg
     5161 gtttgaatca acaaatcgca acactgacac aagaaaaaac aaatgtagaa actcagttaa
     5221 cagatacaca aaagaaatta gacgaagcaa ctgctgaaaa ctcaagcttg aaacaatata
     5281 ttcaaaagtt agaaaaagca aaagatgaag tggaagatac agctgataaa tctcagcaaa
     5341 ttgttgaaga acatttgcgt aaataatctt tcttaacaag taaacctaaa ttagtaaaga
     5401 agcaacagta atctatactg ctgcctcttt tttttagtac atgtccttca tcttcgcagg
     5461 aagtgccaat atgattttaa gaaaggtaat agacaaaaag tcaaaaagat aaaacagagt
     5521 ttcttcttta caaagggaat gtatgttcgt ataatggtat tgaggtgaag gttatggaga
     5581 aaaaatatgg aacagtagat aagatccgta ctttgcaagt tagtgccaga ccgttgatcc
     5641 gtttcagttt aaatggggtc aattgtttga ttgcttccca tagtttgaac ttcctagctg
     5701 aagtagacga agggatgaag ttagtcgtta caggttatta taataataga aagcaatttg
     5761 ttgtgagatc ctatcatgtg ttagggaaac ccaaaattgt ggtagaatat gagaaaagtc
     5821 tatacccaag aaaaaaggtc cgacaagatg aaagaataaa ctagaaaaca agcggctgag
     5881 acaaaaatgt cctagccgct tgttttggaa agatggatgc agacgatggg atttgaaccc
     5941 acacgagcat acgctcacaa gaacctcaat ctatatataa ctacatgtat ttaagtattt
     6001 gtgatgaatc actaaacttt ccaaactcat ttgagggata ttgcttaaag attgaacaga
     6061 tcaattcgaa gccctattag tattcgctca atggggggat cagaggccat aaaaagagac
     6121 tagtcattac tagtctcagg tagtggaatt gtaggatttt aaacgttcct ggagagtacc
     6181 ggtatgtaac tcgaagaggt gattatcata gtcgtaaaag taaattgaag aagcctcgcc
     6241 tttcacacga gaacgtcctg gttcgacagt taaacctaac atttggattt ttgagacaaa
     6301 cgaatctata tcttgttcgt caattttaaa agcaatatga ttgtatgtct ttggcagttt
     6361 attcgacgaa ttctgcatta ctgcaatcca taggtcctta actaagaaaa atttttctgg
     6421 atataatgaa aatttttttg ttgactatcg tagatttctt ttgcatcaaa taaggtattt
     6481 aacaattaag tatttaaaag ataactttta agttatcttt aagtaatgct taaagctatg
     6541 ttatactcta ttaggaaata taagaaggag tataaaaatg aacaaacata atagaaaaat
     6601 aacgaataac aaagaactga tcttgttact aatagggatt tggggtatct ttttactctt
     6661 taatatgtat atgccgacta tgagagcgga tgacgtagtt tatgcaagta gattagatga
     6721 acttggctat ttgggtgctt cgatagaaca ttataagaca tggagttcaa gaattattat
     6781 agaactattc ttaatgttct tttctaaaca ctttatgcta tggaaattat taaattctac
     6841 gattatgtta ggaattgtag tattactttg caagtatgtt tttgagaaaa taaatgttaa
     6901 aaatttatta ttggtttgct ctatatactg tttaatacca ttgacagtaa tgggggaaac
     6961 cggttggaga gcaaccacct tgaactatca gtggccagta gcatttagtt tactaacttt
     7021 ttatccgttt tttcaactct taagaggaga agaaataaat agaaaaatat attgggtaag
     7081 tattccttta ttgatatttt taactaatca agaacaagtc aacgcctgtt tttttgttct
     7141 aacaagtata gttagtttat atttgattgt caatggacga tataattata agttatcagt
     7201 attttcaata ataagcttag cagaattaat tttctctttg acaactccag ggaatgcatt
     7261 acgtgctgca catgaaatta ataaatggtt tcctgaatat aaaaatttca actttcttaa
     7321 taagttagat ttaggtattt catcatttgg aaagccgttt tttttagaca tgaatatctt
     7381 atttttattg ctatttttct taattttcct tctaacatat aggaaatgtc aaaactatta
     7441 tgtgcgtata ttgactgcat taccgttttt cttaaacctg attatttatt ttggtaatac
     7501 aatgggtcaa agctttactt acgtcaatgg aaataaaagg gcaatgattt ggagtagtag
     7561 taacctgaat aatctcttta cagagttagg aacgaaactg tcactttttt atccaggtac
     7621 ttggatagct actttagtag tcttggcatt attactatgt ttaattgttg gaatttattt
     7681 aagttttgac aataagaaaa cttctatttt tttagttatt ttgatgataa tgggattttg
     7741 ttcaaggtta attatgggat tttctcccac agtatgggct tccggaatgc ggacgtacta
     7801 tatactttat gttgttatcg ccatcttagt actaatggca gttaaagagc taatgaaaag
     7861 catgagtgta caaaaaaatg aatttatgca atttggatta accgtgcttg gtatttgtac
     7921 ctttataata acggtgataa acagataata tacaaaaaat ccctcgcttt agtattaggt
     7981 gagggatttt actcgttttt cgattaattg ttttaacact tgcaagtttc tacgagtagc
     8041 ttgtttccga ccttcatata ctcacctcag gctgtttaca tgattgtacg ttctttttgt
     8101 aaaggataca gtgatatgta tataatggca taaagaaacg ttcaaacaaa aaaactcgtg
     8161 aagcaaaatt aacttcacga gttgtagtgc agatgatggg actcgaaccc acacgagcta
     8221 acgctcacat gaccctcaat catgcttgtc tgccaattcc aacacatctg cgtaataata
     8281 acacactaat gatagcgagg ttttacatag ttgtcaagct caaaaatggg aggacttatt
     8341 aagatggaga aatatgacgg agaattttca ggactgggaa tgatcttagg tgtattgatt
     8401 ggacttgcat tcggacgttt tttgcttggt tttatgctag gaatcatttg tggaattgca
     8461 atggattggg cagcgaatct atggaatgac tatcatgaca attaagttct caacgcttgt
     8521 ttgtgttttt ttaatactta cgtttttcct aaataagtgg caaaattttc taggatggag
     8581 tatgatattg aaatagtaaa taacaaaaaa tttggaaagt tctaactttt tgaaatgagg
     8641 gattggctaa gtgatgagag aatttgaaaa atccaacaaa ttagaaggtg tcagttacga
     8701 tgttcgcggt cccgttctag aagaagcgga gcggatgcaa gaagagggca ttagtatttt
     8761 aaaattgaac acgggaaatc ccgcaccttt tggatttgaa gcaccaaacg aagtcattcg
     8821 tgatttgatc atgaatgccc gtgactcaga aggctactca gattccaagg ggatcttttc
     8881 tgctagaaaa gcaattgagc aatattgcca actgaaacat tttccgaatg tgacgatcaa
     8941 tgatatctac accggcaacg gagtgagcga attgatcacg atgtgtatgc aggggttgct
     9001 ggacaatgga gatgaagtac tggttccaat gccggactat ccgttgtgga cggcttctat
     9061 tgcattagct ggaggcacac ctgttcatta tatttgcgat gaagaggcag aatggtatcc
     9121 tgatattgat gatatcaaat caaaaataac gagtcggaca aaagctattg ttatcattaa
     9181 tcctaataat cctactgggg cactttatcc aaaagaatta ttagaagaaa tcgttgaagt
     9241 agctcgacaa aataacctga ttatttattc cgacgaaatc tatgatcgct tagtgatgga
     9301 tggtctagaa catattccga ttgctacatt agcgcctgat ttgttcgttg ttacgctgaa
     9361 cggtctatcc aaatctcatc gtgtagcagg atttcgagta ggctggatgg tcctaagtgg
     9421 agataagtca cacgtcaaag gctatataga agggctgaat atgttgtcct ccatgcgttt
     9481 gtgttccaat gtattgtctc agcaaattat ccaaaccgcg ctaggcggtt atcaaagtgt
     9541 cgatgagctg ctcctgccag gcggaaggat ctatgaacaa agagaatata tctataatgc
     9601 aatcaatgat attcctggtc tttcagccgt aaaaccaaaa gctgcttttt atatattccc
     9661 aaaaattgat acaaagcgat tcaatatttt ggatgatgaa aaattcgttt tggacttttt
     9721 acatgaacat catattctac ttgtacatgg tggtggattt aattggaacc agccagatca
     9781 ttttcgaatc gtttatcttc ctaaaatgga tgatttgaaa caaacagcta agaaaatgag
     9841 agaattccta gcaacctatc gccaaaaatg attttaaaaa ccaaattttt cagtaggagg
     9901 gccacagaca aacacactga ggctctcttt ctagtggagt tttcttaaat aactaaagta
     9961 tattatttag ttgcaagtct tttcttgaag tttgcgactt cctaaagtaa aatacgaata
    10021 gaatgcgaat acgcatattt tggggaggat acttatggaa acgttaatcg agtcctttgg
    10081 aacgatcatt aaagagattc gaaaagaaca aaaaatgacg caaaaaatgc tttcgcatga
    10141 tatttgttca caaagtgtat taagtcggat cgaaaataat gaggaactgc cgaatgtatt
    10201 ggtgatgatg cagatttgtc aacggctagg ggtaacgatt gatcatgtga tgaatctctc
    10261 tcgaaaaact actcggacaa acgataaatt atttgaactg ttggatagtt gtttccaaaa
    10321 acgtgattat cggaagttag aacaagtact aaaatcttct gatatttctg aaaatctgta
    10381 tcaagcaact gactttcaac gctattatta ttatctcggt gtttgcgaat tcacgttgcg
    10441 tcaaaatatc tctcagtccc tttattattt gaaagaagca ttaagctact ctagccaaaa
    10501 agaaagaatc catgtttctg acacagaaat acaactgatt agttgtatcg gaaaaatata
    10561 tggtgtggca ggaaaaacgg ctgaagcacg ctactatttg agtcgaagca tccaactgct
    10621 gcatcaatca gctgatgaac gatcaaaaag tgaattttcg caaatttttt ataattatgg
    10681 aaatttctta ttccatcaaa atgaaatcga cgaagcgtta gaacaggtga atcaaggaat
    10741 ctattgggct caagagaaga atagctacta ttacctcaat gatttatttg tgcttaaaag
    10801 cttaatatac aagcgtaaag aagaaccaga aagagcagca ttctatgaag gcttagcaca
    10861 agctgtgaaa caaatttcaa aaaatttgta aatggtgtag accattcttg acttcttctc
    10921 taaataagag tactttagag gtagaaaaac aaagggagag aatagaatga gaacttttat
    10981 ctatgctgac aaattctttt tagcgtcagg tgtcaaacat gcaggctatc ttgaaattac
    11041 cgatggactt tttggtgaat acaccaaaga actacctgag ggagaagtca acatcattga
    11101 ccaaagcggc aaatggattg ctcctggttt ggtagataca catattcacg gctatatgaa
    11161 tcatgatgta atggataatg atgcagaagg catcaaagtg atgtctgaag gtctgttatc
    11221 ttgtggagtg acttcatttt taccaacaac attgacgtca agcaaagaac gtttaaaaga
    11281 tgtagctgag acaatcggca aaatgtatca ggaagcacca ggagctaaaa tcaaaggaat
    11341 ctattttgaa ggtcctttct tcacagaaga acacaaaggg gcacaaaacc caagttattt
    11401 cggggatcct gatttggata ctttccatga atggcaagaa gcttcaggtg gtttaatcaa
    11461 aaaaattgct ttagcaccgg aacgtcaagg tgtagaagag tttgtcaaac aagtgactga
    11521 agaaggagta gttgtttctc taggacacag taatgctaca ctagaagaag cgcaaaaagc
    11581 tgttgaagca ggcgcaagtg tatttgtcca tgctttcaat ggtatgcgtg gattgaatca
    11641 tcgcgaacca ggtatggtgg gtgcgttatt atctttagaa catgtctttt ctgaattgat
    11701 ttgtgatggc catcatgtac atccaaatgc tgcgggaatc ctaatggaaa aagcaggaca
    11761 tgatcatgtc gcattaatta cagactgtat gatggccggt gggatgcctg atggaaatta
    11821 caatttaggc gaatttccag tagtagtaaa agaaggaaca gcacgtctag aatctggcaa
    11881 cttagctgga agtatattga aattgaaaga agcaattaaa aatgttgtag actggggatt
    11941 agcaacacca gaacaagcag tcatgatggc tagtctgatt ccagcaatca gctgtaaaat
    12001 cgatgataca tgtggaaaaa tcgctcaagg acgcgatgca gactttatcg tattgaaccc
    12061 tgatatgaca ttggaagcaa cttatctaga tggcgtggaa cgtttccacg cataatataa
    12121 tttaagttaa ccttaaaact aaacttctta agtttggttt taaggttttt ttgtgttcta
    12181 gtaaaaatca ctttacataa gtttcaaaag tttctttaat ttcctattgt tcaataagta
    12241 acaatgtaaa gataaaatta aaaatgttaa tacgtattag caaaataaaa gcttttttta
    12301 ttgtgaataa atgaacataa ttaggaggag aaagatgaac ttatttgaca tttttcgatc
    12361 gactttgaca gatatgtcga ttgtcagtgc aatcacttca acagttttta ttatattatt
    12421 agggtttgtt tgtagaaaaa aggggatttt ttctgaatct gttggaaaaa ttctatcgaa
    12481 agtcgtctta acagtggctt tgccagcact ggcatttcga gctttcatgc aagatatcaa
    12541 tacagaatta ctgatgcaag gaatcaatgt cttgatttgg ggattagtta tttatattat
    12601 tcttattcta gtttcaaaac cttttttctt aagctataaa ggggataagc aaacaacgtt
    12661 gcgtgtcctg acgatctttg gttctacgac ctttttcgga acgcctatcg tcagtgcgat
    12721 ttatggtcct gtcggcgtga tgtattcctc aattttcaat attggctacc gaatcttttt
    12781 gtattcttat ggatacataa aaatgagtgg cttgaagatg gaagctaaaa atatcaaagc
    12841 gatgttcgtc aatccaatca ttatcgctac ttttgccggc cttttcattt ggctgttcca
    12901 agggtatctg ccacaagtgt cagttacagg tgcagaagga gagataagcc aagtagcgtt
    12961 tttgcgaatt gaccagactg caccgtggct atttcagccg atggattatt tagctggttt
    13021 aagttcacca ttggcgtggc tagcaatcgg cgcaacatta ggtgaagtaa gcttcaaaaa
    13081 agctgcatct gaaaaaactt catggtatta cagtctagtc aaagtggtat ttgtacctgc
    13141 attgaatatc gtgattctag cagttttgac ggcaacgaat attctaccag tcaattttga
    13201 agcactggca acaatcgtta tcatgatggc tacaccagct gcaacagtgg cagcagccta
    13261 tgctatcagt tttgataggg aggcactttt agcctccaat gcatccttac tgtcaactat
    13321 ttttgctgtg gtactgacac ctgtatggat cgttgtatta caaatgattc atggcatagg
    13381 tttattttca taagaaatgg aggaagaaag tatgagaatc gcatgttacg gtgttcgtcc
    13441 gaacgagata gatttttttg aaaaattgaa tatttaccat tatgatctca gtctttatga
    13501 agaactgttg acacatgaca atatcgtgtc agcaaaggac catgatgctg tattgttgag
    13561 gggaaattgt atagcagatg agaccaatct tgcaaaaatg caggaatatg ggattcgtta
    13621 tgtttttaca cggactgtgg gagtcaatca tatcgatcta caagcggcag ctgattttgg
    13681 tatgacagta gcccgtgtac catcgtattc gccaaatgcg atagcagagt tatctttgac
    13741 ttttgccatg atgcttttga gaaatactgc gtatacgacg atccgaacat ctttcaaaga
    13801 ttttcgagtg gatgagcaaa tgtttagtcg tgaaatcaga aattgcacag tagggatcat
    13861 aggaacaggc aggatagggt tgacggaagc tgttttgttt aagggactag gagccaaagt
    13921 gatcggctac gatattcatc agacagaagc tgctaaaaaa gtattgacgt tccactcgct
    13981 tgaagagtta ttagccaaaa gcgatattgt cagtattcat gtacctcata tcccaggtga
    14041 aaacgatcaa atgatcaatg aaggattttt agctcaaatg aaaaaaggta gcatcttgat
    14101 taatacagcc cgtggagagc ttcaagataa tgaagctatt ttaagagctt tgaaaagcaa
    14161 tcatttagct ggttttgcaa ccgatgtttt tgcaaatgaa acagaggttt ttttccgatc
    14221 cttcaagcca tgggagacga ttccagaccc tgctatccag caattagtag aaatgtaccc
    14281 tagagttctt gtaaccccgc atgtaggttc aaatacagat gaggcattat caaatatgat
    14341 ttcgacaagt ttcgagaact ttcgtgaaat aatagaaact ggaaaaacga aaaatgaggt
    14401 atctcttcct aaagcccgtc aattaagata agaaaacatg gatattcgtc aataaaatgt
    14461 ttaaaaatag tccaagtaaa cagttgttac ttctgtatac ttggactatt ttgtatggga
    14521 taattcgatg actcaacagt aatctgtgag ctttttccgc ccattctttg tcaattcata
    14581 acaggttagc ggctggccga tttccaaata tttgattctt tcgttcaata tatcttgttc
    14641 tactaaaaaa ttcaaatatt tcttgacgga tatacgtgac agtgagcttt tttgagccag
    14701 ttcttttgtt gaaaaaatag tatcttcttt tagaataaat gcacttattt tttttaaagt
    14761 cagttttgac agacctttag gcaaatcgat gaatgatggt atttcgtgaa tgggagtggt
    14821 gtggttgaat acttgatcga tcaatttctg atcggctttt tcagtcgttt ggattgtctg
    14881 agagaaagca agatactttt cgatcgccag ctgaaaacgt tgcaaagtga acggtttgat
    14941 caaataatct aaggcaccgt agcggatcac attctttatg gtttcgatat catctgcagc
    15001 agtgatcaaa atcactggga tatcatttcc ttgctgtcta agccaagata aaaaatggat
    15061 accactttcg ccaggcatgt atacatcaag cagcagcaga tctgcgtttt tttgtctgaa
    15121 atattttttt ccttcttcta catttctgaa actttcgatt acgttcatat tagggatgct
    15181 ttccacaaaa gtacgattca attctgcaac cattggatca tcttcgatta tcaggacatt
    15241 tgtcatcatt ttgatcttcc ttttttggta attctagata gacgattgta ccgccatttg
    15301 tttgtttgga aaattctaaa aatccaccgt ggcgtttgac gattgaatgg atgagattca
    15361 atccaaaccc acgattgatt cccttcgtcg aaaaacctag cgtaaatatt ttttctgaga
    15421 tttcttctga tatcccgaca ccgccatcgc agacggtcac gatgaatact tcagcttctg
    15481 tgtcatagtt taaatgaatc cggacctgtc cgtttctttc atgtttttgt agcacggctt
    15541 cttttgcatt atcaagtaag ttccctaaca cttgcaaaag tgaatggatg gagtcatcca
    15601 tctccatttt agggataaaa gaatcgcttt ctaacagcat ttcaacagat tgttctttgg
    15661 cttcgtttat tttgcctaat aaaaaaccgg caattgcagg tactttgatc agacttgaga
    15721 cttggccaat ctctttttta taattttgat ttagctgctg gataaaatgg gtgacttctt
    15781 catacttctt caaatcaatc aaaccagaaa tgacatgaag tttattcatg aattcatggg
    15841 tttgagctct catggagttt atatattgtt tcgtcccccc aagctcctcc attaatttca
    15901 ttacttctga tcgatttcgt aaagttgcga ctgctcctaa aaaacggtca ttgctaaaaa
    15961 gaggtgtaac gctagaaata gtttctatcc catttaaata catggattga gtccgaacgg
    16021 tcgtttgatg ctcaatcgtc tctttaaaca aaacgtcata tagattatca ctgatttttt
    16081 tcctacttcg aaatccaata gctgctctgt ttggtagaaa atgttttttg caggtgtatt
    16141 gatcaagagg atagttttat cagaagagat ggctaagatt ccttcatcta cttcattttc
    16201 gataatcgtt ttttcactta actgttctgc tatttcttgt ggttcaagtc caagcagtac
    16261 ttttttcatt ttttgggcaa gaaaaacggc gcctatgatg cctacgctaa gtccaataat
    16321 caatgaaaaa acaatcattt tttgtccatc tttgacttga ttgtcaatcg tgttgtttgt
    16381 taagccaaca caaactgcac caataatctc ttgatcataa taaataggga cgaaatagcg
    16441 gaagccgaac cctaacattc cttctttttc agagaaaaat tcttgtcctc caatcacttt
    16501 tttcacatct tcatgacttg agaaaggaag accgatgtaa cttgggtctg cgtgagaata
    16561 tcgaatgcct tcagggtcca tgacaacgat aaaatctaaa ttcgatgcaa tcatgatttc
    16621 atttgttatt ccttgaatcg catgtgtcgg gtcaccagtc tgcagctttt tttggatgag
    16681 tttatcattg gcaacgattt tagcaatgct agagatttta tctgacacac tagatcggtt
    16741 catcgaaaga ataaaattcc tgatcaatat ggcagaaaca atcaaagaaa cgaatgtgga
    16801 gaaacaaacg acaaggatga tcgtgttctg gagctttaat ggttttttct taaaaaaagg
    16861 aaacataggc tacctcgaaa gaatttattg tgaaaatttt aacatgaaaa caaaaattta
    16921 acaaattgtc aacgataaac acaaccttcc tataaaaaac accactgagt cggtaaaaga
    16981 agctatacag aattttcaaa cattggtatg atagacgtag aaatggaagg aggcgtgtat
    17041 atgatagggg gaatcgcact tgagacgatt tattggtata cattgctgat ttgtgcagga
    17101 ttggcagtcc tattgattat ttttggagat gtattcgatt ttgatggccc gcttgatccg
    17161 atgcttatta tcccttggtt aactttcaca agcttatttg gttttctagg tgagcgttta
    17221 acagatcaat catctttatt tttatttatt gctagtggag gcatatcgat agtgttggta
    17281 tttttgctta atttctatat attgatgccg atgaaacatg ccgaatcaac gatgtcgtct
    17341 tctgaaaaaa ctctggaagg acaagtagct gttgtgatca caccgatccc tgtacgaggt
    17401 atgggagaaa ttcagttgaa aagtgttacc ggttccatta gtcgtccagc ttgtttttac
    17461 agcccccaag aacagacagc tgagcgggga acgaaagtat tgatcattga gatcaaggaa
    17521 agaatctgtt atgtcatgcc ttatgaaagc atgataaaag cttaaaggag cgtgtgttaa
    17581 atggaaatac cagttgtttt tatcgcgatc gcaatcgcag ctttgatttt actgatgttg
    17641 ctgatcgtct ttgtagcgaa gtaccagaca gcaaaaccgg atgaagcgtt gatcatcagt
    17701 ggaagttatt taggaagcaa aaatgtgcac attgatgaaa gcaataacaa aatcaaaatt
    17761 gtgcgtggag gtggcgcatt cgtcttgcca gtttttcaac gttcgaatcg gatcagtcta
    17821 ttatcgagta aattggacgt ctccacccct gaagtgtata ccgaacaagg cgtaccggtg
    17881 atgtgtgatg gtacttcgat catcaaaatc ggttcatctg tagaagagat tgctacagca
    17941 gcagaacagt ttttaggcaa gacaagagaa gaactggaaa atgaagcaag agaagtatta
    18001 gaaggacact tgcgctcgat tcttggttct atgactgtag aagaaatcta tcaaaatcgc
    18061 gataagttca gtcaaagcgt acaagaagtc gcttctgtag accttgcaaa aatggggttg
    18121 atcatcgttt ctttcactat caaagaggtc agagacaaaa acggttatct tgactcttta
    18181 gggaaaccaa gaatcgccca agtcaaaaga gatgcagata tagcagaagc ggaagctcta
    18241 aaagaaacgc gtatcaaaaa agctcaagca gaaaaagaat cacaagcagc tgaattacag
    18301 cgacaaacag agatcgcaga atcattaaaa gaaaaagaac tgaaattagc aacctacaaa
    18361 caagaacaag atgttgctaa agcaaaagca gaccaagcct acaatttaga aagtgcccga
    18421 gcacaacaac aagtaatcga acaggaaatg cagattaaaa tcgtagaacg ccaaaaacaa
    18481 atcgaactgg aagaaaaaga aatcacacgt cgtgaaaagc aatatgattc ggaagtgaag
    18541 aaaaaagcag atgccgatcg gtacgctaaa gaacaagaag cacaagctca aaaagtcaaa
    18601 gaagtgactg aagccgaagc agaacgattc cgtgtggaag cactggcaga agcggaagcc
    18661 aataagacac gtttagctgg acaagcagaa gccgaagcag cactagcaaa aggaaaagca
    18721 gaagccgaag caaaacaaaa gatcgccaat gccttcaagg aatatggcga agcagctgtc
    18781 ttgagtatgg ttatcgatat gctaccgcaa ttgatgcgcg aagcagcaca gccgctagga
    18841 aatatcgaaa aaatctctgt tgtcgataca ggaagcagtt caggagaaac cagtggagcg
    18901 aaccgagtga caaattatgc cacaaatttg ctttcttcta ctcaagaaac actaaaagcg
    18961 acaacaggac ttgacttgaa ggaaatcgta gagaatttgt cgacacgtgg gactactagc
    19021 caagtaaata tgtatgaagc gccaaaagag gcagaacaaa actcgatgaa ggaagaataa
    19081 agtagtacac ttcaccgttc aatcaaaaat ggaggaagac tttttaaagt tcttctctcc
    19141 atttttttat tttattgcaa aagaaaatta gtcctcgcta tttttctttt gtttttggat
    19201 cttagcttct atctcttgta aatttgttct tagcaatcgt tctttctttt ggagttcgac
    19261 caaatcttct gatagttctt ccttcttttc tgtttcagga ggacgaagaa agaaattgat
    19321 accgattgca atgaatgtcc ctataaaagt atctagtaca cgttggatag catacaaagc
    19381 ggattcgcct tgagggatgc tcaacgcaat cagcaacaac gtagctattg cggaaatgat
    19441 accggaatta ttgttaatac catctgacac gacgatcaca ataataacta gaataggaag
    19501 caagatcaat tctaccaaaa aatcattgtg aaaatagctt ttgacaaaaa aataaatgat
    19561 agctaatgta ccacctaatg tattgccgat gatcctagac tttccaaaag aaacggttgt
    19621 agtcaagtcc tgacgcaaag aaaaaacagc agataatgca gcaatcatcg ggattcctcg
    19681 ttgagtcaaa tgaaaaaaca agatacataa aaaaacagct aaggcacttt tgatcgtacg
    19741 catacctagt cgaaaacggc caaattgcat agagattctc ctttttggtc tttttactat
    19801 catacctaaa cttttgtctg atagctagtt tttcattatt caagtataat taaaataaaa
    19861 aagggggaag taaatgcgta taggaatgcg aacagtcaaa acagtgatca gtgtcttctt
    19921 ttcgatggtc acagcgggta gcttatcgct gttgtattgg ccagctgcag ggatcattgc
    19981 attattaagt gtaggaaata ccaagcgctc tacattgcgt acgggcatga atcgaatcgc
    20041 agcctttata ttagctacgg caacagcact gttcagtttt tttgctgttg ggtatactat
    20101 ttttggcttc agtctttttt tattcctgtt catcccgctt gcctctcgct tcaaattaac
    20161 cgaagggata gtagtaaatt cggttttggt cactcattat ttagtggagg aaaacatgtc
    20221 ttggcagctg atcggcaatg aagcgggatt aatggcgatt ggattagtat ttgctttgct
    20281 tgcaaatgtt tacatgccag atcgtacgaa gcagctgaag gaaaaccaaa tacagatcga
    20341 aaaagaattt cgaaatattt tgagacaaat ggcagaattt cttttatcag aaaataaaga
    20401 agatgtgcag atcaaatgcg aacatttact gacattcatt cgcgaaagcc aagaagatgc
    20461 cagagaacat caagaaaact actggctcag acagccgcta tactacgaaa cttacttctc
    20521 tatgagaaga gcacaggcca atgtgattaa agacatgctg gaaaatctag agagaatcca
    20581 gcaaccagct tattatggaa aacatatcta tggtttattg atttatactg cagaaacatt
    20641 ttcagagtca aatgatggca gacaaatttt aacacgtatt gaagaagttt atgtattgta
    20701 tcggcaaatg cctctgccga ctagtcgccc tgaattcgag gatcgagcag aattgttcca
    20761 gtttttgcag tcttttaaaa gttttattga aatcaaggca gaattctccc aacaaaaaat
    20821 ccaaaaatag aggttgtgaa ttcagctaat attcaggtca aaaagctgat ttcacaacct
    20881 cttctttaaa cggtgttctc ctgtcaactc ctcggtcaca agtcacaata ttcaacaaac
    20941 atgagaagct gttttttgaa attgctcctt gtgactcgga gctgaacggt aggctcacaa
    21001 cctctttctt aaacggtgtt caaaagcttt cctctgcggt aataagtcca gccaaacgaa
    21061 aaataggagg agctattttt gtttggctgt tcttattact tgatgcagaa cagctttttc
    21121 acaacctttt aaaattacat atttttccat agaacttgca tttgatccaa gatttcgatg
    21181 acatcttttg tcaagaagag agagcgattg ggcaagtcac cttcaatcat gtgtaccata
    21241 ttctttattt cgtaattcat tgcttcagat gtattcccgc tttcgatgaa ttcctttgta
    21301 ccatctgtaa ataagatctc cgcacgatca gcacgcgggt aatcagcaat cgtcacataa
    21361 ccgttctcaa aggcaacgat accgactttt ggcatttttg cctgaaaagt caagctgaca
    21421 gcagcaagtt cattttcttt gttacgcaag atagtgacgg attgttcgtc cacgcctgtt
    21481 tcaaaaggaa ccattgtaga ggcaactact tcaggttgag agctcaaaaa gaaacgagcg
    21541 aaagatacag catacgtacc gatatccaac agagctcctc cagctaattc tggattgaag
    21601 aaccggtttt tagggtctgg ttctttgtaa ctgccgaaag gtgcttggat catttttaaa
    21661 gcccccagtt ttcctgtatc cattattgag cgtaactgct gataaagtgg catgttgaag
    21721 attgtcatcg cttctgctaa gatcaaattc ttttcttctg ctaagcgcat agcgtcagcc
    21781 agttcttttt tattcatcgt gatggctttt tcacatagaa cgtgtttgcc tgcttccaat
    21841 gctgccaaga tatggtcgat atgctgtcta tttggtacag caatgtacac gatgtcgatt
    21901 tcagcatcgt ctaacatttc ttgatagctt ccgtaagctt ttggaatatt gtaacggtga
    21961 gcgaagtttt ccgctttttc tgaagttctg gaagcaactg ctgttagttc gctcgtctct
    22021 tgattgaagc tttcggcaaa ctcatgtgcg atggtaccaa gaccaacgat tccccattga
    22081 tattttttca tctgaaaacg ctccttcata gttggattga gtttcttgtc tgtctattat
    22141 tataacaaaa atcacgaaga ataaagagga gaggacttga aacattaaaa aaaatgaaag
    22201 cagcgattta agaggtgctt tttgatcaaa acttaagtag aatagttaaa aggaggggat
    22261 aaacgtgcat caagtatata ttgatcttat tatgagtgta ttattagaac agtttgaaac
    22321 tgaaaaaaat tttttagatg aatacttgct gatcagtcca gaacaatggc aaaaatggaa
    22381 agatggcgag aaaaatctgc catctgaagc gatgcagaaa gtaaaaaatc ttttcactga
    22441 ttatgaatgg atgctaacgc aaaagatttt gcgtcaaacg attatttttc ccgaaaaacg
    22501 aaacgttgcc gttgcagagt acaagcaaat caaaacacgc atcgcgcaaa aatggcttag
    22561 cagcaaagca gcaaaagtcg agctgatccc tttgaaaaat aatcagcagg ctggtagata
    22621 ccttgacttg aaagtaagta ttcaatatga tgaatggggc tatgatgata ttttgaattt
    22681 tcgtctgcca gcagatgttc aaaaacagat cgaaggagaa aaaatcgaac ttctcgattg
    22741 ggtgaatgaa aatctggaag aaacttacgt tcatgaggcg aatggggaac gtgaagaaca
    22801 acgaaaggat ttttcaagtg aataaatttt taggatttgg agcagttgtg attatgatcg
    22861 cagcaatggg ctatgttatt tttagcgatg atgattcgca tgttcaagcc ggcaatcaag
    22921 cagtcgaacg aacaagcacc aaaacaactg ctagtaaaga aaagaacagt caaacaacca
    22981 gcagcaaggt aaaggacaaa gaaacaaaag atcgtacgtc caaacaattg ccagatgtta
    23041 aaacagacga ttggaagcta gttttggttg gtccagatca caaattagaa aaagaaattg
    23101 atgagtctac tcaattggca tcattaagta acggatactt gatcgacaaa cgcattgctc
    23161 cagaatatga agcatttgcc aaggctgctg aagcagctgg atttccatta gtgatggtct
    23221 cagcttaccg gtctgtatca tcccaacaac aagttttcga acaaaatgtc caagatgtga
    23281 tgagtcgtca aggtgtatca gaagaggagg cgacaaagat tactaaagaa acgattacgg
    23341 agccaggata cagcgaacac cacacaggtc tggcagtcga tgtcgtcgat caaaactggt
    23401 acaacagtta tccttctact gtattggatg caagttacgg agatcagcct ggagctaaat
    23461 ggatcgcaga taatgctgcg cagtatggat ttatcgttcg ttatccaaaa ggacacgaag
    23521 atataactaa gatcacatat gaaccttggc acttacgtta tgtcggtaaa gaaaacgctg
    23581 agtatatcac caaacaccaa ttgacgctag aagaatatct gaatgaatta aatgaaaagt
    23641 aggaattgat catggcgaaa aaaaagtgga aaaagcaaaa gcgcaagaat atccgttggc
    23701 cagcagtggt tgccggtgta gcagtgatac tcttagcctt tgttttttcc ttgaagaatc
    23761 tgtcttctcc ttttactgat aatcagcaag aaaatgaagc gcaatcccat cagcaattta
    23821 ttgaacggct tgttccgcat gcccaagaac tacaagatgg ttatggagtg cttcctagta
    23881 tcattttagg acaagctata ttagaatcaa attggggaaa aagtaccttg gctagcaaat
    23941 acaataatct tttcgggatc aaggcttatg gggatcagaa gaaagtaagc ctggaaacaa
    24001 aggaatttgt taacgaagaa tggataacta tccaaggaga cttcaaagtg tatgattcat
    24061 gggaacagtc catggatgac catacacaac tgttcgtcca aggagtagat tggaatcctg
    24121 cgctgtatga aaaagtgatt acggccacca actatcagga agccgcacaa gcattgcaag
    24181 atgcaggtta tgcgacagac cccggttatg cgcaaaaaat catccaggtc atcgagacgt
    24241 atcaattgaa tcagtatgac cggagatagc aaaggagaag tggctgatgg cagaacaatt
    24301 tacgccagaa atcatgacgg aattgagaaa agatatcgta agagtaccgg aaatcatttt
    24361 agaagcaagc gggatcaaga tatttggaaa attgatccgt tcgatcatct ttactacgga
    24421 tattgcgatc atacgtaata caaatgcgaa tgcagtaatt gctgtgtatc cttttacacc
    24481 tcatccagcg attactaaaa gcattattga ggcagctgac attccagttt tctccggtgt
    24541 aggtggtggt ttgacacaag gtgctcgttc ttcctacatg agcatgttcg cagaagcgca
    24601 aggttcgtta ggggtcgttt tgaacggtcc tacgccttta gagacagtaa aagaagtttg
    24661 tcgagtagtt gatattccag ttatcagtac ggtaacgtcg aaatatacag aaatagatga
    24721 aaagttagca gctggtattt cgattatcaa tatcagtgca ggtaaagaaa ctgcagaagc
    24781 agtacgccat tttcgtaaaa aatatcctga acttcctatc attgcgacag gtggtccaac
    24841 ggaagaaagt attgctgaga ccattcaagc aggagcaaat gccatcacat atacgccacc
    24901 aagtagcgga gagcttttta gtcgtaaaat ggaaaaatat cgggaaactg agaaaaagaa
    24961 tcatgaataa caggtgagaa tcaaaagacg aggaagagca gatagtggct cttcctcgtc
    25021 ttttgtactc cagtactatt tgttcagaat tcaaaaaaaa gagaaatttt tttgtttttt
    25081 tacctgtaat gtacataaga agagttaaaa ccttttgtta ttattagcat tctaacaaaa
    25141 aaatgtcttg atttattgaa gcgctttcta tagaatgaaa aacgaataaa aactaaaaaa
    25201 ggcacccgac aatttagtag cagctaaatt gtcggggctt gaaaagtcag cgggaactga
    25261 ccaatcaagt tgccatagtc aatgcttgtg catcgacgtg tatattgtac ctaatttcat
    25321 acagaattac tagttatttt tctgtttttt agggaagagc gcacacgaag aagcatgctt
    25381 atttagctat gtcaaggata ttgggcagtt gtctgatatc cttttttctt tgcgtaatta
    25441 tcctcccatc attgaagcaa gaagtgtttt tagcgatgaa agaagaatca gcactatcta
    25501 atttttcttg aaagtattga gttgttcatc ctaggcagaa cgataaggca agtcttggtt
    25561 tttattcccc ttgaaaaaga tcgtttaata aatatgttct gtataaaaat acagtatatt
    25621 gaaaaataac aatggaagta gaggtgcaga gaatgaagaa aagaatgaga atcatcttag
    25681 ctttaagtgt tatgttattc ttcttgacgg cttgtgggca agaagatgac aaaaaaatca
    25741 cgctgaacat ggcttggtgg ggttcgcaag tccgacacga tgcaacagta aaagtaatcg
    25801 agctttatga agaaaaaaat ccccatgtga cgatcgatta tgaattttat gattatgaag
    25861 gatattacac aaaattaggt acactagcag catcagatga tatctgggac attttccaac
    25921 tgaacaatca atttcctcaa tacatcaatc agattgaacc attagatgaa tatgtggagc
    25981 aagggataat tgatatttct aatgcagatg aatcctattt agcaacgacg acttacgacg
    26041 ggcaattagt tggactgtct aacggggtga attcgtttgc gattgcttac aataaaaata
    26101 tatttgaaca attaaatatc cctgaaccag cttcaaattg gacatgggat gaatttgaac
    26161 aaatcagcta tcaaatcact gaagaattag gaatctatgc tatttcaaga ttcgaagatt
    26221 ttatagctgg ctgtattatt caaataccac aagttgagaa aggattgaat ttttataatc
    26281 gggaagatct atctaacagc ttgggatttg aaaatccaga ctatctaaca gattttttcc
    26341 agctgcgtaa aaatctggta gacaaagggg cccatccaga accaggtggt ggagctgcaa
    26401 gtacgacgga tctgtctttt caggcggtta gggatagaga agcggcaatg gtgtttctat
    26461 caagtaacca gttgacagaa atattgtcag gggctcctga agatatggag attcagttga
    26521 ttaatccacc tcgcagaaaa gcagatggag aatctggtat ccctttacgt tcttcgcaaa
    26581 tgctgagtat ggcaaaaagc tcagcccata aagaagaagc tgctaagttt attgatttct
    26641 ttcaaaacag tgaagaagcc aatgaaattt taaaaggaga acgtggtgta ccaatcatgt
    26701 cgaatattcg agaacagctc gctcgtgcag atgatgcaac tttgcaagcg acatttgatt
    26761 atttagaaat gatttccgat atggacaacg gcgaagtgaa tgtctttgat agtgctttga
    26821 atccggaaat tcaagaccaa tacaatcttt tagttgaaaa agtcatttat gaagaagtaa
    26881 cagcagaagg aaggcatgaa aaaagaaaaa ctaaaacggt tcttggatca agacaatgtg
    26941 gtcggttacg tatttgcttc tccattcatc ctaggatttc tattgataac agtgataccg
    27001 atgatcttgt cactttatta ttcgttcacc gattatcgtt tgggaaatcc agtctcatgg
    27061 atcggcttgg ataattatat aaagatgctg agtgatgccc gttttttgaa ctccatccga
    27121 gtcacttttc tctatgtatt tgcatctgta cctgtgaaac tgatctttgg tctattgatt
    27181 gcttatcttt taacgaggaa tatcaaagga acgactttct ttcgttcttt gtattatgtg
    27241 cctagtttga tcggtggcag tatttctatc gctctagttt ggcgggagtt gttttctaaa
    27301 aacgggttag tgaatatttt cctggaacga ttaggcgcac ctgcgctttc ctggtttagc
    27361 gatcaaaaac tggtcatgat ccctcttgtt ttgatgtctg cttggcaatt cggttcggca
    27421 atgatcatct ttgctgcagg tatcaaagag atcccaggct cgtattatga agcggcagag
    27481 atcgatggag cgaacaaatg gcagacgttt tacaagatca cgttacctct tctttcgccg
    27541 atcattttgt ataacttggt tatgcagaca atcgcggcgt ttatgacttt tacgcaggca
    27601 tttatcatta cccaaggtgg tccgaatgat gccacgaact tttatgctct ttatgtgtat
    27661 aaccaagctt tttcttttaa ttctatgggg tatgctagtg ctatttcgtg gatcatgctg
    27721 ttgattatgg gagctgtcac ctttctgatt ttcaaatcat caaaaaaatg ggtcgttgtg
    27781 tgaatgagag gaggaagaaa tgaataatac agtaacgaaa tggtttcaat acggttttat
    27841 tttgctatta ggtctagtca tgatttatcc ggtcttatgg atgatcgcag gttcttttaa
    27901 aacggatcaa gagattatga gtggttcgct gaatttgatt ccagagactt ttcaatggga
    27961 aaattacgca aaaggatggg cggggtttgc tggaatttca ttttttactt tctttaggaa
    28021 ttcgtttgtc atctctgcag tttcgacgat agggacagtt ctgagctcga cttgtattgc
    28081 ttacgcatta tcgagaatcg atttcaaagg aaaaagattc tggttcgtcg tcatgttagt
    28141 aacgatgatg attcctgcac aagtgatttt gattccgcag tttattattt acaatcggct
    28201 gaatttggtt gggacgtatg tgccgctgat tttaccacat ttttttggac aagcattttt
    28261 catttaccag atcatgcaat tcatggtcag catccccaaa gagttagatg aatcagcgat
    28321 tattgacgga tgcagcaagt atagtgtctt taccaaaatt atttttcctt tgctgaaacc
    28381 ttcgatcatc acgacgatca tcatccagtt ttattggaaa tgggacgact tcatgggacc
    28441 attgatttat ttaaacaagc cgagatctta cacggtatcg attgccatca aattatttgc
    28501 agacgctgga tctagtacga cttacgcgag tatgtttgcg atgtctacgt tgtcgctgct
    28561 accggttttt ctgattttct tattcttcaa taaatatctt gtccagggga tcagtacaag
    28621 cggtctgaag ggctgaatat gaaaaagcca atagaagaga tgtttcttta caagctcaac
    28681 tactatgtct gtcggatttt tttaaccagt cttatcagta atgctctttt cgctttagtt
    28741 ctaggatgtt tgttcacttt aggattagtg ctgtctgctc gtgttgtcta tctgatttat
    28801 tttttgatga tgtatccttc ttattatcta tctgtccatt acttggcaga aaaaaaggta
    28861 tgttggagga aagggaaaat caaacggatg tgtttcatca ttacgttgtt tcagctgttt
    28921 gtcattattt ttgcagttga ttggtattta ttagtgggaa agattggagc ttggtatctg
    28981 attccttttc tattgtgtct gttgcttttt gtatcccata gtttttttag tacgctaatt
    29041 gatatctgca aagaagataa agcagatagt cttgtttatt tgatcaaaag gaatatctgg
    29101 ctgttgtcaa aaaacgtcaa ggggtttctg tggtatttat gccttttgta tggactgttc
    29161 tggtgtatac agcctgattt tttactcact gcattactgt atccagggct gccatttgtc
    29221 acagtatttt gcttaaaaga aaaggagaat gactttaaat gaagaaaaaa tatgtacaag
    29281 tcggacttgg tgggagagcg cgtttttttt atgaggcaat tgcctctacg tatcatcaaa
    29341 cttgtgaact agtcggtttt tgtgatgtat ctcaaacaag gatggattat gcaaatcgga
    29401 tacttgtgaa caagtatgga cagaaaaaag taaagacatg tatgccatca caatttgagc
    29461 aactactgga tgacgtacgg ccggctgaag tcattattac gtcagtagat cgaacgcatc
    29521 atgattatat cattcgtgca atgaaaaaag gatatgatgt tatttgcgaa aaacctctga
    29581 ctatcgatga ggaaaaagct caagaaataa taacggtcca aaaacagacg ggaagaagcc
    29641 tgcgagtcac ttttaattat cgttatgcac cgtatcacac gaagataaag gaactgatag
    29701 aaagtgatgt gattggtgat gtgttttcta tccacttcga atggctgtta aatacgcaac
    29761 acggggcaga ttatttcaga agatggcatc gaaataagca caatagcggc gggcttcttg
    29821 ttcataaatc tacccatcat tttgatctag tgaatttttg gttgaacagc cgtccccaaa
    29881 aggtatttgc attcggtgga ttaaactttt atgggatgca taatgcggag aaacgcggag
    29941 tagaaatatt ttacagtcga tgctatggaa gcgaactagc taaagctgat ccatttgcta
    30001 tagacctgtc agccaatgag gaattgcgag aaatgtattt agaagcagaa aaagacagtg
    30061 gatatattcg tgaccgcagt gtattttccg atgatatctc tattgaagat acactaggcg
    30121 taacggtcaa atatgaaact ggtgcgattt tgacgtattc gctaaacgcg tatatgccgt
    30181 gggaaggatt caatgtttct ttcaatggca gcaaaggacg gatcgaaatg aaagtgatcg
    30241 aaaagtctta catcaatgca ggtggagata aaaaggatga aggagcagcc gttcttcagt
    30301 ccatccatgt ctatccgatg ttcggtgagc catatgaggt aatcgtcgaa caagaagcgg
    30361 gcggacatgg cggaggagat ccgcgtttat tagatgatct atttggtgaa gaaaaagaag
    30421 aagatccgtg gaatcgggca gcaactcacg tagatggtat tttgtcgatt ttgacaggaa
    30481 tcgcagcaaa tcattctatt gcttcaggaa aagcagtagt gattgacgag cttgtcagct
    30541 taaaagaata agaatagatt tacggaaatt acttcccgta gtttataaaa tcttgcctta
    30601 ttgccgatag gctgtctttt gaacactatt tattcggaaa aaggggctat gacatgcttt
    30661 ttgtcatgcc ttctttttat gtgggctgct tttttagata ggagaaaata aaaataacta
    30721 tttaaacccg aacaaaaagg aataatgcat taaaaatcat tagattttac aaaaaaataa
    30781 gtaaaccacg ttgtcagttt cagaaagtag tgttagactg ttttcaatat ttgaaacaac
    30841 attttttatt caaatatggc aaactgcctt cacgtgcaaa acagttgcaa gaaaatgaat
    30901 ttacactatt attagtttta catacctact tatatatcgt cataatatgg atgacagttt
    30961 ctagccagta ccgtaaatgc tggactataa gtaaaagatt ggctatttca cccttctttt
    31021 cttatgataa agtggagggt gtttttcttt aacagaaaca tagtaggttt ttgcacaaaa
    31081 gcggttattt cggtattaat aaatgataaa ggagcaaata aagtgaaaga attagttgaa
    31141 cgtattcaaa aagacggacg tatattaggc gagggcgtat taaaagtcga cagcttcgtc
    31201 actcaccaag tagaccctgt attgatggaa caaatgggaa aacggtttgc agaagtattt
    31261 aaagaacaag gaatcacgaa agttgtgacc attgaagctt ctgggatcgc accagcttta
    31321 tttgctgcaa gagaattaga tgttccaatg atcttcgcta gaaaagcaaa aagtttgacg
    31381 atggatgaag aattattgac agcttctgtt tattctttta cgaaacaagt gacaagtacg
    31441 atttctattt ctcgtaaatt tttatcttca gaagacaaag tattgattat cgacgatttc
    31501 ttggcaaacg gacaagcggc aaaaggattg atcgaattat gccagcaagc tggagcgcaa
    31561 gtagaaggaa tcgggatcgt catcgaaaag tctttccaag atggacgtca gttacttgaa
    31621 gaaatgggat tgagagtcgt ttctcttgcg cgtattgctt ccttgacgaa tggacaagtt
    31681 gagtttcttg aggaggacgc ataatcgtgg aaaaagaaac acaaaatgga aaagctgcgg
    31741 ttttagggct gcagcatctg ctagcgatgt atgcaggagc agtagcggtg cctcttttga
    31801 tcggtacagg actaggcttt aatcaagaac agatgactta tttgatttca attgatatct
    31861 ttatgtgtgg attagcgaca ttattgcaat tgacagttaa ccgatttttc ggaattggct
    31921 tgcctgtcgt tttaggatgt gcgatccaag cagtagctcc actgatcttg atcggaacca
    31981 atgaaggagt aggagctatc tacggatcta ttatcgcttc tgggatcttt gttgtgttag
    32041 tttctggtgt cttttctaaa attaaaaaac tattccctcc tatagtaaca ggaacggtta
    32101 tcaccgttat tggtttgact ttgattcctg tagcaatcga gaaaatgggt ggaggtaatg
    32161 caacagcagc tgattttggt gacaaaacaa atctgctact tgcatttgtg acgattctcc
    32221 taattgtagg cgtgcaaatg ctggcaaaag ggttcattcg ttcaatcgcg gtattgattg
    32281 gtttagtcgg aggaagcatc ttagctgctt tcatgggaat gattgatctg agcgcgattg
    32341 gagatgcgcc gttgttccat gttccacaac ccttttattt tggtaaacca actttcgacg
    32401 tatggtcgat tttactaatg atcatcattt ccatggtcag catggtagaa tcaacaggtg
    32461 tatattttgc acttggtgac attactggga aaaatgttgg agaagaagaa ttgaaaaagg
    32521 gatatcgtgc agaaggactt gccgttatct taggcggtat tttcaataca ttcccttata
    32581 ctggcttttc tcaaaatgtc ggattggttc aactttcggg aatcaaaaca agaaaaccca
    32641 tctatttctc tgcattcttc ttgattgttt taggattatt ccctaaaatc ggtgcgttag
    32701 cacaaatcat ccctgagccg gtacttggtg gaggaatgtt agtcatgttc gggatggtgg
    32761 cagtccaagg aatccgtatg ctgttggaag ttgatttcag caatgataaa aatctattga
    32821 ttgttgctgt ttctgttgga tttgggctgg gtttcaatgt tacgccgaca ttattcagtc
    32881 aaatgccgca aacattgcaa atgttcactg gaaatggcat cgtaatgagt agcattacgg
    32941 ctatcatatt gaatttgatc tttaatggat tgaaaaaaga tgaagaaata ttggaagaag
    33001 ccttcaagaa gtaatagagc aaaagaaagg ttgtgaaatc agcgttttga cctgattgtt
    33061 agagaaacaa acggaaaaat agttcctcgt attttgccgt ttgtttgagc taataccgag
    33121 ggtcagctga ttgaacaccg tttatccata aaaaggaatg tgacctaact tttgtcacat
    33181 tccttttttt catctttaat aaacacgaac aataaaatgt ttttctttgt taacattcga
    33241 aatttctttg acatcaaaaa aagtgattgt taaaatatga atgtagaaaa actgggagga
    33301 aaaacaatga cagcaaaaat ctctgtgatt atggggagta cttcagattg ggaaacaatg
    33361 aaagaagcat gtcaaatatt ggatgaattc ggtgtgtctt atgaaaaaaa ggtcgtttct
    33421 gctcatcgaa ccccagattt gatgtttgaa tatgcacaaa acgctcgaaa aaaaggaatc
    33481 aaaatcatta ttgcaggagc agggggagcc gcgcatctgc cgggtatggt cgcatcaaaa
    33541 acaactttgc cggttatcgg tgtaccggtc caatcacgaa cattaaatgg tttggattcg
    33601 cttctttcga ttgtccaaat gcctggagga gtacctgtag ctactacagc aatcggaaaa
    33661 gcgggagcaa ttaatgctgg actacttgcg atacagatgc tttccatgta tgatttagaa
    33721 ttggaggcta aattggctga aagacgttca atgttggcta aaactgttat agaaagtagt
    33781 gatcaacttg gttaaacctt tgttgcctgg ttcaacgatc gggatcgtcg gtggcggtca
    33841 attaggtcgt atgatggcga ttagtgcaaa agaaatgggc tttaaagtag gtgtgctgga
    33901 tcctgtgagc gattgtccag ccgcacaagt tgcagattgg cacattgaaa gtgtctatga
    33961 tgacaccttt gcgctgaaag agcttgccag aagaacagat gtcattacct atgaattcga
    34021 aaacgtcagt gtagaggcgt taaatgcgat tttacccatg tcatttattc ctcaagggac
    34081 agacttgctt gcgattacgc aagaccgcct gttagaaaaa tcttttttgg aaacgaataa
    34141 catcgtgatt gctccttatg caacgatcgt cagtccgacg gatatccaag atgcgattga
    34201 cgggatcggc tatccttgtg ttttgaaaac cactcgaggc ggctatgatg gcaaggggca
    34261 gtatgttttg aaaagtcggg cggatctagc tcctgccatg gaccttttaa gagaaggaac
    34321 ctgtgtttta gaagcatgga tcccgtttga aaaagagatt tcgatcatgg tggcaggaaa
    34381 tggtcaagga gatttcacga cttttccggt cgtggaaaac atccaccaca acaatattct
    34441 tcatgaaacg attgctccag ccgcgattga tcaggatgtg atcgaggagg ctgagcgtat
    34501 tgcccgtgtg attgcagaag cagtgagcct ttcaggtgtt ttaggggtag aaatgttttt
    34561 aacaaaaaca ggcgggctat atgtgaatga attagctcct cgtcctcaca attctggaca
    34621 ttattctatt gaagcttgtt ctatgagtca gttcgatgcc catattcgag gtatttgtgg
    34681 ttggccatta ggagaaaaag tacgtttgat aagcaatgca gtaatggtca atgttttggg
    34741 agaacaactg ctggaaagct atcgcttgat tgagaataag ccagactggc aattccattt
    34801 ctatggaaaa aaacaagcaa aaaaaggtcg caagatggga catatcacta tcttgacaga
    34861 agacatccag caaacgctgg aagaaatcaa acaaacgaat atttgggatt agaggagaaa
    34921 aaacatgtta gaacgttata cacgccctga aatggggaat atctggacgg aagagaacaa
    34981 ataccgtgcg tggctcgaag tcgagatttt agcagatgaa gcttgggcag aacttggtga
    35041 aatcccgcaa gaagatgtga aaaaaattcg tgagcatgca tcatttgata tcgatcggat
    35101 cttggaaatc gaaaaattaa cacgccatga tgtcgttgcg tttacacgag cggtttctga
    35161 aacattggga gaagaaagaa aatgggtcca ttatggtcta acaagtacgg atgtcgtaga
    35221 tactgcttat ggctatctca tcaagcaagc aaatgatatt ttacgggaag acttgaaacg
    35281 ttttacggaa atcattggcg aaaaagcaaa agaacacaaa tatacagtga tgatgggtcg
    35341 gacacacggt gtccatgctg aaccaactac ttttggattg aaacttgcca catggtattc
    35401 agaaatgaaa cggaacatcg aacgatttga acatgcggca aaaggagtag aagcaggaaa
    35461 aatctcagga gcagtgggaa cgttcgcaaa tatcccgcca tttattgaag agtatgtatg
    35521 taaacaattg gggatccgtc cacaagaaat ctctactcaa gttctccctc gggatctaca
    35581 tgctgaatat ttctcagcga tggcgcttat tgctacaagt attgaacgtt ttgcaacaga
    35641 gatacgcgga ttgcaaaaat ccgaaacacg tgaagtagaa gaattttttg cgaaaggtca
    35701 aaaaggttct tctgctatgc cgcacaaaag gaatccgatc ggttcagaaa atatggctgg
    35761 tctagccaga gtggtgcgcg gacacatggt cactgcctat gaaaatgtca cattatggca
    35821 tgaaagagat atttctcatt cttcagcaga acgaatcatc ttgcctgata ccacgatttt
    35881 attgaattac atgttaaatc gttttggcaa tatcgttaaa aatctaacgg tctttcctga
    35941 aaacatgaag cggaatatgt ctgcaacatt tggtttgatt tatagccagc gcgttatgct
    36001 gaaattgatc gataagggaa tgacaagaga agaagcttat gatcttgttc agccaaaaac
    36061 tgcgtacgct tgggacaacc aagtgcagtt ccgcccgctg ttagaagaag atccaaccat
    36121 cacatcggta ttatctaaag aagaaataga cgatgccttt gattatcatc atcatttgaa
    36181 aaacgttgac gtgatattca aacgagtagg tttggaataa agaagagtga gttgcaaaat
    36241 tcaattaaaa tagaatagca aaaataacca aaaaagaagc agatccgtta gcggttctgc
    36301 ttcttttagt ttattatatt ttcttatcaa taataggaac ctgattttaa aagcggctac
    36361 ctctttaccg tgcctcttta catacaaaaa atagaggaat taagcgtaaa tggcttggct
    36421 attataacta aaatgctggt tatggagatg ttgcagcaag ttttcgagaa aagcgtattc
    36481 catctcttgt ccattgaata agatctggat acgataatga tttttgaaac gcttctgttc
    36541 atgaatctga ctgattgaaa gttcgccttt gtgttctgtc tgataaagaa aagtcctttt
    36601 acggcattca gcacagcgct tggttgctcg gcggattttt cggaatgtat aaaaactagt
    36661 atctttattg cataaataaa cgtctttgat cgacagcaaa tcattatata gacaggaacg
    36721 ttcttcagct gttaattctg ataaaaaaag ctgaccttgt tttattgcat cttccatcgg
    36781 caagtgcctc ctcgaaccca ttatccaaat ttttagttaa ttctaatata ccatcattaa
    36841 gtataaatgt aaacgcttgt tgatggtata agattttcgt ttctgataaa acacgaacat
    36901 taaaagaatc taatgcctta aatgtacata tttttgttga tttataaaaa gatgattgct
    36961 aaaatttaaa gtgaaaaaac gaacaaaaaa cgatgtattt gcttttaatg taaataaaaa
    37021 ggagagacgt acatggagaa gaaagaactt ttgtatgaag gaaaagccaa aaaaatatat
    37081 cttacttctg atgagaactt gatttggata gaatatttag atcaagcaac tgcgttgaat
    37141 ggcgcgagga aagaccagat aagtggaaaa ggagaattga ataatcaaat cacaagtgca
    37201 attttccgtt atctgactca aagaggaata aaaaatcatt ttattgaaca attgtctaaa
    37261 accgaacaat tagcaaaaaa agtacaaatt atccctctgg aagtggttat ccgaaatatt
    37321 gcagcaggaa gtttctcaaa acgtctaggt gttcccgaag gtaaagaatt catcaaacca
    37381 atcattgaat tctactataa ggatgaccag ttggatgatc catttatcaa tgaagagcat
    37441 atctatttct tacaacttgc aaaaccggaa gaaatcagtg agctgaaagc ccaagcgttg
    37501 gctattaatg aaattttgac cgaattattt gctaaggtcg gtttggtttt agtagatttc
    37561 aagctagaat ttggccggtc gaatggacaa attttgcttg cggatgaagt aacaccggat
    37621 acttgccgat tatgggatga acgtacgaat gaacatatgg acaaagacgt ttaccgcaga
    37681 gatctaggtg atttgatccc agtctatcaa gaagtatata accgtctatc ggaactaact
    37741 gaagaaaagg agcgagtatg atgtatttcg taaaagtata tgtaacctat aaagattcag
    37801 tgttagatcc tcaaggggag gcagtaaaaa aagcagtcca tcgtatggga tatgaaacaa
    37861 tcgaagatat ccgaatagga aaatactttg agatacaact tgctgatgca gaagaaccaa
    37921 tcgatacagt gattgaaacg atttgtgaca agctgcttgc aaatgtaaat atggaaacat
    37981 accgatatga aatcaaaaaa atggaggaag tataacatga aatttgcggt catcgttttt
    38041 ccaggttcaa attgtgacgc agatatgtta tgggcagttc gcgagatcat gggcgctgat
    38101 gccgaatttg tacggcatga tgagtcctct ttagaaggat ttgatggggt tttactgccg
    38161 ggaggattct cctatggtga ctatttgaga tgcggtgcga ttgctcggtt ttctacaatc
    38221 atggaagaag tgattcgttt tgctgaagaa gggaaaccag tattcggtac atgcaatgga
    38281 ttccagatat tgacagaagc aggacttttg ccaggtgtac tgagacgaaa tgagtctttg
    38341 cattttattt gcaaaactgt accattgaaa gtcaacccca agacggcctt tacttcagag
    38401 tatgaagccg acgaaataat ctatcttcca gttgctcatg gggaaggaaa ttatttctgt
    38461 gacgaagaaa cacttcaaga actaaaaaaa aataatcaga ttgcctttac ttataatgac
    38521 aacatcaacg gcagcgtgga aaacattgca gggattacga acaaagcagg gaatgtgtta
    38581 ggaatgatgc ctcatccaga acgtgcagta gaagacttgc taggatcaga agatgggaaa
    38641 cggttctttg cttccatttt aaaaaacttc ggaaaggtga ccgtatagat gaaaacattg
    38701 aaagaaccga cagctgaaga gatcaagaaa aaaagaatct atgccgaatg gggattgact
    38761 gatgaagaat atcgaatgat cgaagaagat atcttaggaa gaatgcctaa ttatacggaa
    38821 actggactgt tttctgttat gtggagcgaa cattgttcct ataaaaattc taaaccggtt
    38881 ctacgcaaat ttcctactag tggacctcat gtgttgcaag gccctggaga aggcgcaggc
    38941 atcgtcgaca tcggtgatgg actagcagtt gtcttcaaag cagaaagtca caatcatcct
    39001 tcagccgtgg agccttatga aggagctgcg acaggagttg gcggaatcat cagagatatt
    39061 ttcagcatgg gggctcgtcc aattgcgttg ctagattcat tgaggttcgg cgaattagat
    39121 aatccaagaa cgaaatattt actagaagaa gtagttgcag gtatcggcgg atacggcaat
    39181 tgtatcggca taccgactgt aggcggcgaa attgcatttg atccttgtta tgaaggaaac
    39241 ccattagtca atgcaatgtg cgtaggtttg attgaacaca aacatatcca gaaagggcaa
    39301 gccgctggcg tcggtaattc gatcatgtat gtaggtgcaa aaacaggtcg cgacggtatc
    39361 cacggagcaa cttttgcctc agaagaattt aatcaagaag aagaacagca acgttctgct
    39421 gtacaagtag gcgatccttt tatggaaaag ctgctgcttg aagcgtgttt ggaattaatc
    39481 ttgcatcatt cggattctct aataggtata caggatatgg gagcagcagg acttgtttca
    39541 tctagtgcgg aaatggcttc aaaagctggc tcaggactta tattgacatt agatgaagtc
    39601 ccacaaagag aaactgggat gacaccttat gaaatgatgt tatcggaatc acaagaacgg
    39661 atgttgattt gtgttaaatc aggagaggaa gaaaaaatcc aagaactatt tcagaagtat
    39721 gatctagatg cagtcactat cggtaaagta acagacgacg ggcaatatcg gctatatcat
    39781 caaggagaag aagtagcaaa tctgccagtc gatgcattag ctgaagatgc accagtttat
    39841 cataaagaaa tgaaagaacc agctcgaatc gtagaatttc agcagttagc tccttatcag
    39901 ccagttatcg aagaacctgg acaagtattg cttgatctgc tgcagcaacc gacgattgct
    39961 tcaaaacgtt caatctacga aacttatgat tcacaagtac aaaccaatac agttgtccgt
    40021 ccaggaagcg atgctgctgt gatgagggtt agagggacga ataaagcgtt agctatgacc
    40081 acagactgca acgcgcgcta tctgtatttg gatccaaaaa tcggaggaca aatagcagtg
    40141 gcagaagctg ctagaaatat tattgctagc ggtggtaagc ccttggcgat caccgactgc
    40201 ctgaactatg gttctcctga taaaccagaa gttttttggg aattatcgac ttcagctgac
    40261 ggaattgctg ctgcgtgtga aacgttagga actcctgtta tttcaggaaa tgtctcttta
    40321 tataatgaaa cggatggtca agcaatctat ccaacaccaa tgattggtat ggtcggattg
    40381 attgaggatc ataaacatat cactactcag gaattcaaaa aatcagggga tctaatctat
    40441 atattaggta agacgtttgc agattttgat ggcagtgaat tacaaaagat gcagcttgga
    40501 agaatcgaag gtgtgatccg aaactttgat ctatccattg aaaagagaaa ccaagagtta
    40561 gtattgacag ccatccaaaa cggattgatt gaaagtgctc atgactgttc agaaggtggc
    40621 ttggcaattg cactggcaga atcagctttc aaacatcagc tagggatatc tgtacagttt
    40681 gaattgtcct ctgctcaatt atttgcagaa actcaatcaa gatttgtgtt gacagtcgca
    40741 ccggaaaata agacacgatt cgaagaaatg atgggagatg ctgctgtttt agcaggtaag
    40801 gtgacggatg aagcgataat cgaaatttca gcaactgacg gtcagatcaa aatagaaact
    40861 gcagtagcaa gaaagtgctg ggaggatgca atcgtatgtc ttatgaagtg aagagtttaa
    40921 atgaagaatg cgggatcttc ggtgtttggg gtcatccaga cgccgctagt gtggcatact
    40981 tcgggctcca tagtcttcag catcgaggac aagaaggtgc aggtatcgtg tccaatgatc
    41041 gaggaaagct taaggggcat cgagatttgg ggctgctttc tgaggtattc aaagatcaaa
    41101 gaaaattgca gcagttaaca ggagaagctg caatcggaca tgtacgttat gcaacagcag
    41161 gcaatggaag tgtggacaat attcagcctt ttctattcaa atttcatgat cagtcgatcg
    41221 gcttagcgca taatggtaac ttgatcaatg ctcgttccct tagaaaagag ttagaggaaa
    41281 atggggcgat cttccattct aactccgata cggagatatt gatgcatttg atccgacgaa
    41341 gtgaagaaac agatttttta aataaattaa aaagtgcttt gaatgaagtc aaaggtggct
    41401 ttgcctattt attaatgaca gaaacggcaa tgattgccgc tttggaccca aatggttttc
    41461 gtccattagc aatcggacaa atgaaaaacg gagcatacgt gattgcctcg gaaacttgtg
    41521 cattagaagt tattggggca cgttttataa gagatgtcca gcctggtgag atagtgatca
    41581 tcaatgacga aggaatccag attgattgct ttacgaagga cacgcagctt tctatttgtt
    41641 cgatggaata tatctatttt gccagaccag attcaaacat cgcagggatc aatgtgcata
    41701 ctgcacggaa aaatatggga cggaatttag cgaaagaatc gccagtaaaa gcagatatgg
    41761 tcgttggcgt acctaattct tctttgtcag ccgcaagcgg ttatgcggaa gctagtggaa
    41821 tcccttatga aatcggatta gtaaaaaatc aatacattgc ccgaacattt atccagccaa
    41881 cgcctgagct aagagagcaa ggtgttcgaa tgaaattatc tgctgtacga ggagtagtgg
    41941 aaggtaaaaa ggtgattatg gtcgatgatt caattgttcg gggtacgaca agcagacgga
    42001 tcgtccatct tttgaaagaa gcaggggcaa aagaagtcca tgtgaggatt gcttctcctc
    42061 ctttgaaata tccatgtttt tacgggattg atattcaaac gcgtaaagaa ctcattgcgg
    42121 caaatcacac gattgatgaa atcaaagaat gtatcggtgc agattcttta ggttttttaa
    42181 gtgaagaggg tctgatcgaa tcgatcggtc tggaaaaaga tgctccgtat tcaggattgt
    42241 gtatggctta tttcaacgga gattatccga cgccgttata tgactatgaa gaaaaatatc
    42301 aggaatcact aaaagaaaaa gtatcgttct tttaaaagca gaccaagaag ggagtatatg
    42361 aattcaatgg caaatgcata cgcaaaagct ggcgtagatg tcgaagctgg atatgaagta
    42421 gtagaacgga tcaagaagca tgtcaaaaaa acggaacgtt taggtatgat gggcgctata
    42481 ggaggattcg gcggttgttt tgatttatcg gtctttcaat taaaagagcc agtccttgtc
    42541 tcggggaccg atggcgtggg gacgaaatta gcagtggcaa tcgaagcaga caaacacgat
    42601 acaatcggga tcgactgcgt agcgatgtgt gtcaatgata tcttagcaca aggcgcagag
    42661 ccgttatatt tcttagacta cttagcaacg gggaaaaatg atccgttacg tttggaaaat
    42721 gtagtaaaag gtgtagcaga tggatgtgtt caaggaaata tggcgctgat tggcggagaa
    42781 acagctgaaa tgcctggtat gtactcaggt aatgattatg atctagccgg atttgctgta
    42841 gggatagctg aaaaatctca attattgaca ggagaaacga tcaacccagg agatgtgttg
    42901 cttggaatag catctagcgg gattcattca aatgggtact ctcttgttcg caaaattttc
    42961 tttgagcagc agcagttttc tttatcatca aaactgccag aattgaaaaa aacacttggc
    43021 gaagaattgc ttgagccaac gaaaatctat gtttcttcct tgcttccgct gatcaaagaa
    43081 aagaaaataa aaggagctgc acatatcact ggtggagggt ttgtagaaaa tatcccgcga
    43141 atgttaccag aatctatggc tgcacaaatc aaacttggct catggccgat tcttccaata
    43201 ttcggctgtt tacaaaaata tggtgaaatt cctgagatgg agatgtatga aatcttcaat
    43261 atgggcatcg gtatggtcct ggcagtcgaa gaatcgaaag taaaagaaat agtacacgaa
    43321 attgagcaag ctggcgaaaa agtttatgaa atcgggaaag tgatcgccaa agaaaaacat
    43381 cgtgtggtgt ttataggaga agaacgatga gaatcgctgt ttttgcttct ggaaacggaa
    43441 gcaactttca agcgctagcc gattatctat cgaaaaaagg attggaatct tcgattgact
    43501 ggttgttttg tgatcagcct gaagcatatg ttttgaaacg tgcaactgcg ttgagtgtac
    43561 cggcagattg tttttcacct aaagaatttg attcaaaaaa agagtatgaa gaagcaattt
    43621 tgcataaatt gaaagaaaaa aagatcgatt tgatcgtatt agctggatat atgcgaatca
    43681 ttggaccggt attgctgaaa aattatgata aaagaatcat caatatccat ccgtctcttt
    43741 tacctgcctt ccctggtttg catggcatac gtgatgcctt tgaagctgga gtaaaagaaa
    43801 cgggtgtcac aattcattat atcgaccaag gtgtagatac tggaccaatc atccggcagg
    43861 aaaaagtgag aattgaacag gaggacacgt tcgattcttt agaagaaaaa atccatagag
    43921 tagagcatcg aatctatcca gaagtgatta gtgaaattat tgaaaatgga ggctatctga
    43981 aatgacaaga gcattgatca gtgtatcaga taaaactggg attgtagcat tcgcccaagg
    44041 tttgattgaa caaggagtag aaatcatttc aacaggagga acaaagcatg cgttggataa
    44101 agctgggatc aaaacgcttt cgatcgaaga agtgacaggg tttcctgaaa tgatggatgg
    44161 ccgtgtcaaa acacttcatc ctaaaatcca tggtggtctt ttaggaagac gcgatctccc
    44221 tagccatatg gaggcaatgg aaacggaaaa tatccaacca atcgactttg tttgtgtcaa
    44281 tttatatcct ttcaaagaaa cgattatgaa accagatgtc gaaatagcag aagctattga
    44341 aaacattgat atcggtggtc ctagtatgct ccgtagtgca gcaaaaaacc atgaatttgt
    44401 cacctctgtt gttgatccat ctgactatga agaagtactt tcagaattac gtcagtatgg
    44461 gcatacttca ctttctttca gacaaaaact agcggcaaaa gtctttcgtc atactgcggc
    44521 atatgatgca ctgatcgctc aatacttaac ggattgggtg gaagaaaaag aaccggaaaa
    44581 attgacactt acgtatgaac gaaaacaaac gcttcgttac ggtgaaaata gccatcaagc
    44641 cgctgccttt tatcagtccg ctcttctgga aaaatattcg atcgcagcag caaaacaact
    44701 ccatgggaaa gaactttctt ataataatat ccgagatgca gatgctgcgc tacggatcat
    44761 gaaggaatat gacgagccaa cggtagtcgc cttgaaacac atgaatcctt gtggtattgg
    44821 gacagcatct acgattttag aagcgtggga agcagcgtat gaagcagatc ctgtttcaat
    44881 ctttggtggg atcatcgttt tgaatcgaga agtagatctg ccaacagcag aacagatgca
    44941 taagctgttt ctagaaatca tcattgcccc ttcttataca caagaagctt tggaattact
    45001 gcaaaaaaag aaaaacatcc gattactgac agtagacttt gcgcaggaag aaaatcaaaa
    45061 tcgagaagaa acggtctcag tcttaggtgg acttcttgta caagatcaag atctggcaat
    45121 agaaaatgaa gaagagtgga aagtcgtgac aaaacgtaag ccgactccag cagaagcaaa
    45181 agcattagcg tttgcttgga gagctgtcaa acatgtgaaa tctaatgcga tcgtactggc
    45241 aaacgaacgt cagacagtag gaatcggagc cgggcagatg aatcgagtcg gttcagtaaa
    45301 aattgcgatt gaacaagcgc aagagaaaat ggaaggtgca gttttagcta gcgatgctta
    45361 ttttccaatg gatgattcgg ttgaatatgc agccaaacat ggtataaaag caatcattca
    45421 gccaggagga agcatcaaag accaagattc tattgatatg gcgaataaat acgatctagc
    45481 aatggtcttt actgatgttc ggcattttag acattagaga ggagtcggat aaatgaacat
    45541 tttggtgatc ggtgcaggtg gtcgtgaaca tgcaataagt aaaaaacttt tagccagtcc
    45601 gaaggtggat acggtgtatt gtgcgccagg aaatccgggg atgacaaaag atggtatcaa
    45661 ttgtgtcgct atcagtgagc gagatcattc agcgttaatc cattttgtta agaaagaggc
    45721 tattgattgg agtatcgttg gtcctgaagt tcctttgtta aatgggatcg tcgatgattt
    45781 tatggcggaa ggattgaaaa tcttcggtcc gacaaaagca gcagctctga tcgaaggttc
    45841 gaaaagtttt gctaaacaat taatggatac ttatcaaatc ccgacagcta aatccagaag
    45901 ttttgaatgt tacgaaaaag cagcagcgta tatcaaagaa aatggtgcgc caatcgtagt
    45961 gaaagcggac ggcttagcag ctgggaaagg tgtagtcgtg gctaccacag tgaaggaagc
    46021 gctggatgcg gcagaacaaa tgttgaacca gcatcgattt ggggatagta gcaaaaaaat
    46081 cgtcgtagaa gagtttttgg caggagaaga attctccctt ttggcgtttg tccgtaatac
    46141 caaggtgtat ccgatggtca tttcgcaaga tcataagcgg gcatttgata acgataaagg
    46201 gccaaataca ggcggaatgg gtgcttattc accagtaccg caaatttcaa aaaagatgat
    46261 tgaaaaagct gtggaagaag ttttaaaacc agcggctttg ggaatgaaag aactaggacg
    46321 ctctttcacg ggtgttttat atgcgggttt gattgctacg aaggaaggac caaaagtgat
    46381 cgaattcaat gcgcgtttcg gtgatcctga aacgcaagtc gttctttctc ggttgaagag
    46441 tgactttgcg caagtaatcg atgatttatt agagaatcgt acagtagagt taaagtggca
    46501 gcaagaagga tacaatgtag gcgttgtggt tgcagctgcc ggttaccccg aagaatatga
    46561 gacaggaatg gttctaccag attttctgga agaagagctt tctgtttact atgctggagt
    46621 ttcctcaaat aacggcaaac tagttggagc aggaggacgt ctttatttag tagaagcata
    46681 cggtgagacg atcaaagaag cacagaaaaa aatttacacc gcgttaatgg ataaaacgac
    46741 agatggaaca ttttatcgta cagatattgg agaaaaagct gccaatagcg aactgatcgt
    46801 gaagtaaatc agctgcttcc atttataaaa aatacaacaa acataatcgg aagcgccgat
    46861 tttagtgaag ccttttcata aagagtgtgg tacacttttg ttgtgtatat aaataaggag
    46921 gagttcagat gaaaaaaatt caattaggta cttctgatca gaaagcagct gcagtgatct
    46981 taggctgtat gcgtatcaac aatgcggaag atccagtaaa agtgattgag acagcttatg
    47041 aaaacggcat tgattttttt gaccatgcag atatttatgg aaacggtgaa tgtgaagaaa
    47101 tctttgcaga tgctctagca aaaacatcaa tcaaacgaga agatcttttt attcaaacaa
    47161 aatgtggaat cgttccaggt gtgatgtttg atttttctaa aaaacatatc attgaatctg
    47221 tagaaaaaag tttgacccgt ctgaaaatgg attatgtgga tgctttactt cttcatcgtc
    47281 cagatacatt ggtagaacca gaggaagtag ctgaagcgtt tgatgaattg gaacggtcag
    47341 gaaaagttcg ttattttggc gtcagcaatc aaaaaccaat gcaaatcgaa ttgctgaaaa
    47401 aatatgttcg tcaaccatta gtagctaatc agctgcaatt tggcttgaaa catactggaa
    47461 tgatcgatca agggattcat gtcaatatga cagatgaagc aagtatcgat catgacggta
    47521 gtgtacttga ttattcccga ttgcatgata tgacgatcca agcatggtca ccataccagt
    47581 atggattttt tgaaggtgta tttatcggga acgaaaaatt ccctgaatta aaccaaacat
    47641 tagaagaact agcagctaaa tatcaaacga caccaactgg cttggcttct gcttggattt
    47701 tacgccatcc agccaatatg caagtgatcg ccggtacgat gaacgtaggg cgaatcaagg
    47761 aaatcgctca agcttcagaa attaatatgt ctcgggaaga ttggtacgaa ttgtaccgtg
    47821 ctgctggaaa cattttacct taaagattgt cataagcagg tcaaaagggc tataaattaa
    47881 aaagacagaa taacttgaaa gctgttttca gcttcgagat attctgtctt tttttattgc
    47941 aaatggaaag agtatcattg tacggatttc gtttattagt tatttttgtt tttaaggaaa
    48001 agctgtgtcc gatattcttt tggagaaatc ccttgttttt tcttaaataa ataactgaaa
    48061 tagttagggt cattataacc aatttcaaaa gcaatatctg aaatacgata gtctgattgc
    48121 tgcaataatt tttttgctaa ttcgattctt tgttctgtca gataatcgat gaatgtttgg
    48181 ttaaacgatt gagaaaaaat cgtactgaaa tgagagggac ttaatgcaac ttgttgagcg
    48241 acccaagtta aggacatatc tggatcggta aaattttctt tgataaaatc caatgcatgg
    48301 ttcaaaattg gctgatattt gttccttgaa ggctgtgagg gcagagcagt taagaaagaa
    48361 atgaacatat aaactgtcga acggtaaagc gagtcgtcgg cgctgattgc taagcgttga
    48421 ctggtggatg tgcaatgaac catttcttgc gggattttct tttctttcag gcagacatgc
    48481 tggagtagct tagtcaagac aaataaacga tataatcgtg ttcgagttgg tgtgccttgt
    48541 ggtttcgtca agtgttcgat gtacttagat acttcgtttt tcgataatga agacaaatca
    48601 agtgagaggt caaagtaaaa gacagaatca actggaagtt tcgcttcttg aatcaaatcc
    48661 tcatagctga taatttgttc tttaagggaa ctggcggttt caagtatcat ctgcgtgatc
    48721 tcaaaagatg caggtaattc gctgattctt tctgcgacag gaccaatggc gacagaaaaa
    48781 ttattttttt gtcccttttc taattcatgg accaacgttt gggctacctg ataactggtc
    48841 tcgatcacat tttcttcaag agaatcaact attaaaaatt tgatgaatcg agaggaaaca
    48901 gcagagaaga tcacttgttg attctcccta aataaaaact tcaaataatc actgagatga
    48961 gcgtaatctt caaagtctgt actgacttgg tttgctgcta ataatacggt catttttttc
    49021 cctgctaaag agcgattgaa agattcagca agtttcaatg tttcactgat cgtaagtgag
    49081 cctttgaaca aaccgttcaa gaaatgattt tttttcaatt cgaaaacaag atcagaagaa
    49141 gtgtgcgttt ctttttgctg caaatctaaa gcagttactg ctttttctat tgcttgtttc
    49201 aattcgctac ttttaatcgg tttaagcaga taatcatctg cctgtacctg gattgctcct
    49261 ttcatataat cgaaatcgtc gaatccacta ataaatatga gacgagtcca aggaagtagt
    49321 ttccttgctt cttttgaaaa agtcaacccg tccatgaaag gcatttggat atcagtcaga
    49381 atgatgtcgg gctgtaagtc gataattgaa gacagtgcca tctcaccatc tccagcttcg
    49441 ccgacataag tgattggata ataagaacca aggacagtta tttgttcccg caagttattg
    49501 cgaatgaggt attcgtcttc gatgataaaa actttataca tgtttgtttc ctccaattgt
    49561 tttgggagat agagggatca tcagtgtgac agttgttcct tctcggtatt tgctgtcaat
    49621 cgtgataggt ttttgatcat ttccgtaata aagaaggaga cgcttgttta cgttgtaaag
    49681 tccgtagccg atactgaaat ctgaatcgat ccctttggct aactctttac gtatttctgc
    49741 cagtttttct gcagggatgc caatcccgtt gtctataact ttgaattgga tcatctcatc
    49801 aattttgcat gcggtaatac gaataaatcc tttgtgacgg acgtacttcg ttccatggta
    49861 tacagcattt tcaatcaatg gctgcaagat catttttaaa atagaaacag gaaaaagtgt
    49921 ttctggtata tcgatcttat agtcaagcat atggccgtat cttattttta agatgactag
    49981 ataatcattg atatggctaa tttctttttc aagaggaatc cagtcttgtc ctttgcttaa
    50041 agacgtacga aaaaagccgg aaagtgcatg ggtcatagtc agagcttctt caaagcagtc
    50101 ttgttcgatc aaagaaataa tggcatccaa actgttataa ataaaatggg gagtaatctg
    50161 agcttgcaat acacgaactt cactttgtgc taaatggtat tgtttcaaag cgttctcccg
    50221 taaagactgg tttagatctt ccgccatttt attcatgcta gcggtaagtg tttgtaattc
    50281 cagtgtttcc ggcagggtta aacgaaagcc aaggttccct gaagaaagtt gttctgacat
    50341 ctcgatcagc tgattgatcg gttcttggac ctttctctct aaaaagcgac gattcttttt
    50401 cgcatagaaa aaaatggtac taagaactcc aagttccact attgttaata gaatgagcga
    50461 gcggacaagt gtcgtatttt ttttgcttgc taagtcgatc tccacacgaa caaaatcttg
    50521 taagatatct gacagcagtt gagtgacaga atctacctga ttgatgatct cttcattggt
    50581 atccataaaa tcttttgcca agatattatt gatcagctcg ttttgataat cttccagtgt
    50641 atcgatcgta cgtagcgata catctaacgt actaatttct ttaagtgtat ttgtattatt
    50701 tttgatctct cgaatatcat tcctaagttc ttctaggata ttttcctcat tgaactcatc
    50761 tacggttact tggccagaaa tcaagtccca catactttcc aaaacttttt gatcaacctt
    50821 caaagcaatt gcatctgctt tttggatatt tgcgatcgtc tgttgataaa tcaaaagctg
    50881 ttgcgaatat aacagtatgc tcagtagcaa agggataaca gcaaatagaa gcatggcttt
    50941 gtttgcttgg gcaaaaatct gttttatgct taaatttttt gtcatgatac ttctccagtc
    51001 aataatttct ggttgggatg ttagctatat tctgctggga aaagacagtc tcggaaataa
    51061 aaatatcttt aggtaatgtt tttcctgttt gaatatttcc aaaataacga gtaacggtat
    51121 tggctacata ccagccgaca tcaggtttac actcgactac acaattgacg attcctgagc
    51181 gtagcttctc gatcatatct tcttgtccat caatagttac aatgatcaaa tcttctccag
    51241 gtttgattga tgtttctttt atcgcagcta aagcaccgtg agtcatttca tcactatggc
    51301 tgtataatac atcgatttcg gataaatcat ttgtttctaa atatcgtttc atctggtctt
    51361 ttcctttcat ccgaataaaa tcaccgacaa ttcggtgaac aatagtcatc ggaggtttat
    51421 ggtcgatggt tccatctttt tttagggttg gtttacgatt gattgattcc aaaaatcctt
    51481 cggagcgtag cttggtcggt gtggagttct ctgaaccagc aagttctagg attctgattt
    51541 cctgtttctt tgaatttgtg aagtgattgc tgacaaataa acccgcccga tttccttctg
    51601 ctttgaaact ggggccaatg tgggtgacga aaagctcggg atcattcgtt tgaatatgtc
    51661 tgtcgacaag tatgacaggg atccctgctc gctttgcttc ttttaataca gagtcccacc
    51721 cggattcttg caaaggagta aaaatgatca tatccacttt ataggcaatg aacgtccgta
    51781 tatcttggat ttgccgttcc tgattcatgt agccatttcg gtagagaact tggtaatctt
    51841 ctttttcaag cgcagtgata attgattcag tatgcttttt ccgccagcta ctctccgttc
    51901 ctgattgcga aaaaccaacg actagtgggt caggtgcttc ctttgtatta ggtgtacacc
    51961 ccatcatttg gtaagcgatt acaacaaggg ctaaaaaaaa taaaacacgt ttcactttaa
    52021 atcactccat ctggatttgt tagtactatc ttatcagata aaagggaaaa tcaaagtcaa
    52081 aaggaatcaa tcgaaaaaaa tccaagaaac ttaaaaaaat ccaagacaaa aaaagtacac
    52141 ctaaaaaatt taaagtaaat cccataaaaa aatcaatgtt aacgctttct ttattagcta
    52201 tactcggtgt atcaattgaa atgggggata aaaaatgaaa aacaaattta gaaagattgt
    52261 tgggactatc gctattttga ccgcaagcat tctattaagt gcgtgtggca attcaggatc
    52321 tgcagatgat agtacggggt atgtaggaat cgctatgccg accaaatcag cagaaagatg
    52381 gatcgctgat gggaacaaca tggtctcgga attagaaaaa ctgggttata aaacagatct
    52441 tcaatatgga gaagataaag tagaaaatca agtagcacaa atagaaaata tgattacaaa
    52501 aggggtagat acacttgtga ttgcttctat cgatggttcg gctttaactg atgtgttagc
    52561 taaagcaaaa gaagcagata tcaaggtgat cgcctatgat cgtttactga tgaattcaga
    52621 aaatgtcgat tattacgcga cttttgataa ttttggagta ggtgtatcgc aagcagctta
    52681 tattgaagag cacttaggat tgaaagaagg aaaaggacca ttcacgattg aattatttgg
    52741 tggctcgcct gacgacaaca acgcactaat caattacaac ggagtgatgt ctgttttaca
    52801 accatatatg gacaacggtc agttggtcgt gccatctgga cagacgagtt tcagtcaaat
    52861 cgctacgctt cgctgggatg gttctacggc gcaagcaagg atggataatc tgttaagcgc
    52921 aaattatact gaccaaacat tggatgctgt tttgtcgcca tatgatccaa taagtttagg
    52981 aatcatttcg tcattaaaag gcgtggggta tgggagtgaa agtaaacctt tacctgtgat
    53041 tactggacaa gatgcgacag ttgctggcgt taaatcgatc attgccgggg aacaaacaca
    53101 aacgatcttt aaagacacac gtatcttagc aaaaaatacg attgaaatga tcaaagcaat
    53161 cagtgatgga gaagaggttc cggtcaatga tactgaaact tatgataatg gggtgaaaac
    53221 tgtccctacg tatttagcaa atacagtatc tgtcgataaa gataattatc aagcagagct
    53281 gatcgacacc gattattata aagaatccga tctgaaaaat taagaggaac caaacaagga
    53341 actaaagaga tctaagtcag agagaggata agaattatgg cggattgtat tttaaaaatg
    53401 gcagatatca ccaaaaaatt ttctggaatc cgagcgttag attgcgtgaa tctgatgatt
    53461 gaacgtgggg aaatccatgc attgtgcgga gaaaacggag cgggaaaatc aacattgatg
    53521 aatgtcttga gcggcttgta tccatatggc tcctatgacg gggaaatctt ttatgatgga
    53581 aagctgtgtc aatttaaaga tttgaaagat agtgaagaaa aaggcatcgt catcattcat
    53641 caagaattag cactaagtcc ttatctttca ataaaagaga atatttttct cggaaatgaa
    53701 caagcagaaa gaggcgtgat tgattgggaa ctgacagaaa agaaaacata tgaactgctg
    53761 aataaagtcg gtttgtctat cgacccgaat cttctagtat cccagattgg cgtagggcaa
    53821 cagcaactag ttgaaatagc taaagcatta tcaaaatccg tccgtctgtt gattttggat
    53881 gagcctaccg ctgcattgaa tgaagaagaa agcgcgaatt tgctgagatt gatccgagag
    53941 ttgaaagagc aaggtgtaac ctccatcatt atttcacata agctgaatga aatcgtggat
    54001 atcgcagatc gaatcaccat tttacgggat ggacagacga ttgagacact agaaaatgaa
    54061 atgatcagcg aagagcatat tattcgcggg atggtgggca gagatttagt tgatcgttat
    54121 cctgaaagaa aaccggatat tggagaagtt tattttaaag tagaagactg gacggtctat
    54181 caccctcttg atcacgagcg aatcataaac gaccacatca acttgcattt gaaaaaagga
    54241 gagattgtag gaatagctgg actgatggga gcgggcagaa ctgaattcgc tatgagtcta
    54301 tttggacatt cttatggaag caagatatcc ggacgtatat taaaggaagg ggcagagata
    54361 tcagttaaga ccgttccttc agccattgca aacggactcg cttatgtctc ggaggaccga
    54421 aaatctttag gactgaatct gctgatggat attcgggaaa acgtgacgct gagcagtttg
    54481 gataaaataa gtcaaaaagg agtattagat aaagaaaaag aagtaactga ggcagaaaat
    54541 ttccggaaaa agatgcgtat aaaaacaaac tccatccatc aaattgtcag tagtcttagt
    54601 ggtgggaatc aacaaaaagt cgttttatcc aagtggctga tgacacaacc cgatattctt
    54661 tttttggatg agccaacccg tgggatagat gttggggcaa aatatgagat ctacacgatt
    54721 atcgaagaaa tggcagcaaa tggcaaagct gtctgtctta tctcttctga acttcctgaa
    54781 atattaggga tgtgtgatcg aatctatacg atgaacgaag gacgtattac aggagaagtc
    54841 tcgcgagaag atgcagatca ggaagtcttg atgaaattaa tgacgaaaga agaggtgggt
    54901 taagttggaa caggcaaaaa atcttacgga taaaaaagag aaaaaactaa atttgaaaag
    54961 tagagcagct gatctttttg gtcgctacag catggtgatc attttagcgg gactacttat
    55021 tgcgtttcaa ttgatgacgg atggtatttt ctggcgtcca ttgaatataa ctaaccttgt
    55081 tctgcaaaat agccatatat tagtactagc tgcagggatg ttattagttg tgctcttagg
    55141 tcatgtggat ttgtcggtag ggtcagtgat ggcgtttgtt ggtgcaattg cgggcatgat
    55201 gatggtcaat cgccaaatga acccgtggat gacagttatc gcatgtcttg cagtaggcgg
    55261 gctgatcggc gcatggcaag gtttttgggt agcttatgca gggataccgg cgtttatcgt
    55321 gactttagca ggattgctga tgtttcgtgg actgacacaa gttgttctag gaggccaatc
    55381 tctagcacct tttccagctg ttttccaaaa aatgtctacg ggttatttac cagacttaag
    55441 cgaaggaagc agccttcact tcttgacatt gattttagga atgatcatca cgatcgtgtt
    55501 agttgttggt caatggcgaa caagaaatcg tcggaaaaat catttatttg aagtggaatc
    55561 tccaacagct ttcttggtaa aggcggtatt tacagcagtc gttgtactag gaataactta
    55621 tatctttgcc tcttatcaag ggtatccgat tattttgatc attcttggtt tgatcgttgc
    55681 agcatatggg tttttgacaa ataaaacagt agcaggtcga cagatttatg cgacgggcgg
    55741 aaatcgaaaa gcggctgaac tatcagggat caaaacaaag cgaatcacgt tctgggtatt
    55801 cgtcaacatg ggagtcatgg cagctttagc tggattagtt ttagctgctc gattgaatgc
    55861 ggcaacacct caagcaggta catcgatgga actggatgca atggcggcag tatattttgg
    55921 tggtgcttcg acttccggcg gcattggaac aatcatgggt gcgattgtcg gtggcttggt
    55981 catgggtgta ttgaacaatg gcatgtcgat tttaggcgtc ggggtagact ggcagcaggc
    56041 aatcaaagga ttgatcttgc ttttagccgt tgtgctagac ttgtataata agaaaaaacg
    56101 aacaacgtag gagaacgccg gttattcaga aaaaagactg tgacaagaca tttgtcacag
    56161 tctttttttc gctaaaagcg aacattcatt gtttcgtttg tatagactaa aaataatata
    56221 tgttttttta ccattcgtat aaattctctt tttgctcttg atttttttgc ttaattgagt
    56281 taaattacat agggcgctaa aaaaagcctt gattaaatta ataatgttcg tgttttgctg
    56341 gatgtgaaca tcaggaggat aatgtaatga aagtatttga ttacgaagat attcaattaa
    56401 ttcctaataa atgtatcgta aatagccgtt ctgaatgtga tacaacggtc acattaggta
    56461 aacatacatt caaaatgcca gtagtgcctg ccaacatgca gacgattatt gatgaaacaa
    56521 tcgcagaatt cttagcggaa aatggttatt tctatatcat gcatcggttt gatgaagcag
    56581 ctcgaattcc gtttatcaaa aaaatgaaaa agcgtggatt gattacctcg atcagtgttg
    56641 gcgtcaagaa agaggaatat tcctttatag agaagttggc agaagaatcg ctaaatcctg
    56701 attatatcac tatcgacatc gcgcatggac atgctaactc agttattgat atgattcagc
    56761 atattaaaaa atatttgcct gaaacttttg tgattgccgg taatgttggt acaccggaag
    56821 ccgtccgtga attggaaaat gcgggcgcag atgcaacaaa agtcgggatt ggtccaggaa
    56881 aagtatgtat tacaaaaatc aagacaggct ttgggactgg aggctggcaa ctggcagctc
    56941 ttagatggtg tgcgaaagca gctcgcaagc cgatcatcgc tgacggtggg atcagaacac
    57001 atggcgatat tgcaaaatct gtccgttttg gtgcgacaat ggtcatgatt ggttcattgt
    57061 ttgccggtca cgaggaatct cctggcgaaa caaaagtcga aaatggaatc gtgtataaag
    57121 aatactttgg ttctgcttca gaattccaaa aaggcgaaaa acgaaatgtt gaaggaaaga
    57181 aaatctggat ccaacacaaa ggatctttga aagatacact agtggaaatg caacaagacc
    57241 ttcaatcatc tatttcttat gctggaggaa gagatctgga agccattcga aaagttgact
    57301 atgtcatcgt gaaaaattcg atttttaatg gagacacaat ctaaaaaata aataaaatat
    57361 taggatgttg tgaaaaagtt gtcctgcatt aagtaataag aacagccaaa caaaaatagt
    57421 tctttatatg ttttgtttgg ctggaattat tatcgcagat acaagctttt gaacatcatt
    57481 attcagaata agggactgtg ataagatttt tgtcacagtc ccttatgttt tgcaaaaaaa
    57541 gtttttcttt tttctcgtaa attcggtaaa atcaaaagaa aaaatagaca atagaaaaga
    57601 gaagcttatg tgggaaaaag tagagaaaaa acaaggaccg atttatcgtc aaattatgga
    57661 acagattatg agagaaatcg aaaacggaag gatcaatcct ggaagtcaac ttgaatcaga
    57721 acgaaaacta gggcaacttt ttggtgtgaa tcgttccacc gttgtccatg cattggaaga
    57781 gcttagggag cttggggtac taacgagcaa acgtggcagc ggatggtttg ttaatcagac
    57841 tgaatggggg aaattcgctg ttccaagaat cgattggcgg caaatgatct ctcctcgtta
    57901 tgagcagacg gactggtatg agcaaaaaat aagagaagca aagcagatcc aaaaaacatc
    57961 gtttcttgat ttatacgcta gcgagatgcc agatgagctt ttgccgaatt ttgagtttcc
    58021 ttcttattct cttgaagaaa tcttagcgga agaaaaagaa atggatcttt taggctatac
    58081 tccgttacgt aacaaaatca aacggtattt ggaaagtaaa tttgaattgg cattttcccc
    58141 caaccagctt cttgtgactg gtggcggtca gcaagcaatc tttttgattt tacaaacgat
    58201 tctttcagct ggagagggga tagctgtaga gtcaccttct tttttttacc gattacctct
    58261 tttcaaagct tcaggaacgc gtttatttgg gataccgatg gatgaagaag ggatcgactt
    58321 agaaaaactg aaagaatcga tccataaaaa taaattgaaa gcagtgctta tcaacccgaa
    58381 ttttcaaaac cctacaggaa aagtgatgag tcaaaaacga cgagaaaagc tagtagaatt
    58441 gtgtcgacag tatcggcttc caattattga agacgatgta tttacggatc tttcatttct
    58501 aaaccaagaa gaagtccttc ctatacgaac gattgatccg gataatgtgt tatacgtcgg
    58561 ttcgttatct agggttttag gaaaaacaac gaaaatcggt tgggtgatag gacctgagac
    58621 ttttatctcg caactagctc aagcgcaaga aatgatggag ttttcgatga atatccttac
    58681 acaaatcaca gtggctaatg tatttaatga agcaattgat ttgaaaatgc aagatgtaag
    58741 aaaaaagttg aaagaaaaaa gccgactgat aagcgcttgg ggagattcac aatcgttttt
    58801 caagcttcat tcaattttag gtggttacta tgcctggatt acttgggatg ggaaaaaact
    58861 agaccccgaa ctagcagaaa aagtagtaga tatgggtctt ggaattgcac caagtctact
    58921 ttttgggcaa gatataaatg gaatcagaat caattacagt aggctggatg aaaaccaaat
    58981 accgttgttt tattcaaaga tgaaaattct agaaaagtgg ttaacagaat aatttcacaa
    59041 agtaaataga ttaaagaata taacagcttg gatttaaatt tcaagctgtt tctttttttt
    59101 cggaaaaaga atacgatata aagaaactaa gaaaaaacca ttatttgtac gtccgttcac
    59161 gatgtgtaaa taaaataaat gtttgcaaca aaactgtata acaccaacgt ttttaaatag
    59221 aaacagatgg ttgtaagttt gattagaaaa tagaaaaaat gcagatgaaa aataaaacgc
    59281 tttccttatt gtgattttat tcactaatgc tgttaatatg cggattggca taatttcctt
    59341 tcatcgtgaa aatttgtgct tttagcttgt atttctcata aatccatgat aatttattaa
    59401 tgaagacggt taaatagtga tacagtttta ttcactgttt caatcgcaga atgaagagat
    59461 tttcttatag atgagagagg aatgtgtgac aaatggcaaa caaaaaagca ccgatcgact
    59521 tccaagcact gctagatgaa gtcaatgcag atttcccagt atatcaagca cttgatcaag
    59581 atggaaacgt agttaacaaa gatttagtac ctgatttatc cgatgatgaa ctcgttgaat
    59641 taatgactca tatggtatgg tctcgtgtat tagaccaacg ttcaacagcg ctgaaccgcc
    59701 aaggacgcct aggctttttt gcaccaactg ctggacaaga ggcaagtcag ctggctagtg
    59761 cttacgcatt tgataaagaa gatgttcttc ttccaggtta tcgggatgta cctcaactag
    59821 taaaacatgg tttaccttta tctcaagctt ttttatggtc tcgaggacat gctgcaggta
    59881 attcttatcc ggaagaattg aaagctctac caccacaaat tatcatcggt gcacaatatg
    59941 tacaagcagc cggtgttgca ttaggtttga agaaacgaaa caagaaaaat gtcgtcttca
    60001 cttatactgg tgatggcggt tcatcacaag gtgattttta cgaagcaatc aatttcgctg
    60061 gcgcttacca tgcaaatgcc gtattttata tccaaaacaa cgggtatgcg atctctacac
    60121 ctcgtgaaaa acaaactgca gcaaaaacac ttgcacaaaa agctgtcgca gctgggattc
    60181 ctggtattca agtcgatgga atggatccgc ttgcagtata tactgtatct aaaatggcac
    60241 gggaatgggc gatcagtgga aatggaccag tcctaatcga aacactgact tatcgttatg
    60301 gaccacatac actatctggt gacgatccaa cccgctaccg tacaaaagac acagatgacg
    60361 attggcagaa aaaagatcca ttgatccgct tccgtaaata tttgacggaa aaaggacttt
    60421 ggtctgaaga aaaagaagaa caagtcatcg aagcaactaa agaagaaatc aaagcagcta
    60481 ttgctgaagc cgacaaagta ccaaaacaaa aggtttcaga tttcttgaaa aacatgttcg
    60541 aagtatctcc acaatcaatc aaagaacaaa ttgcaattta tgaagcgaag gagtcgaaat
    60601 aatcatggca caaaaaacaa tgatccaagc aatcacagat gccttagcac ttgaactaga
    60661 aaatgacgag aatgtcgttg tttttggtga agatgttggg aaaaacggag gagttttccg
    60721 ggcaactgaa ggattgcagg aaaagttcgg agaagaccga gtattcgata ctcctttagc
    60781 tgaatctgga atcgcaggat tgagtttcgg tcttgcgtta gaaggatttc gtccagttcc
    60841 tgaaatccaa ttctttggtt ttattttcga agcaatggac gaagtagtag ctcaaatggc
    60901 tcgtacaaga tatcgaatga gcggcactag aaaactaccc atcacaattc gttcgccatt
    60961 tggtggcggt gttcatacac cagaattgca ttcggataat ttagaaggtt tgatcgctca
    61021 gtctcctggt attcgagtag tgattccttc caacccttat gatgcaaaag gactattgat
    61081 ttcttctatt agaagtaacg atccggttgt tttcctagaa cacatgaaac tatatcgctc
    61141 attccgagaa gaagtgccgg atgaagctta cgaagttcct ttggataaag cagcagttac
    61201 tcgtgaggga acagatgtat ctattatcac atacggagca atggtgcgtg aagcaatcaa
    61261 agcagcagac aatcttgcaa aagaaaatat atctgttgaa atcattgatc ttcgtacagt
    61321 tgcgccatta gatgtagaaa cgatcattca atccgtggaa aaaactggtc gcgtagttgt
    61381 tgtccaagaa gcacaacgac aagcgggcgt tgccgcgcaa gtagtttctg agatctcaga
    61441 acgcgctatc ctttcattag aagccccaat cggacgagtt tctgcacctg atacagtctt
    61501 cccatttgga caagcagaaa atgtctggtt gccaaatgca aaagatatcg aagacaaagt
    61561 aaaagaaatc gcggaatttt aagaaaagaa gggacgaaac aaagtcatgg cttatcaatt
    61621 taaattgccg gatatcggtg aaggaatcgc agaaggtgaa atcgtcaaat ggttcgtcaa
    61681 accaggagat acgatcaatg aagatgatac attactagaa gttcaaaatg acaaatcagt
    61741 agaagaaatc ccgtcacctg ttacaggaac ggttaaaaat gtgattgttc cagaaggtac
    61801 cgtagcaaat gttggggatg tattagtaga aatcgatgca ccaggacacg aagacaatga
    61861 aggggacagt ggtgtagccg cagaatccca aactccagct aaaccggctg ctgaacccac
    61921 tgttgataca gaatcagctg gatcgtcaag cgaaggagtc ttccaattta agttaccgga
    61981 tatcggtgaa ggaatcgcag aaggtgaaat cgtcaaatgg ttcgtcaaac caggagatac
    62041 gatcaatgaa gatgatacat tactagaagt tcaaaatgac aaatcagtag aagaaattcc
    62101 gtcacctgtc acagggacag ttaaaaacgt gattgttcca gaaggcactg tagcaaatgt
    62161 tggggatgta ttagtagaaa tcgatgctcc tggacacaat agtgcaccgt caactagtgc
    62221 gccatcagct gaagcaccaa aagaaaaagt ggaaacatca ggttcagcaa gtgtcgttga
    62281 agctgccgat ccaaataaac gtgtattggc tatgccatct gttcgtcaat ttgcccgtga
    62341 aaaagatgta gatatctcac aagtaactgc aactggaaaa ggtggcagag taacaaaaga
    62401 ggacatcgaa aacttcttgg caggtggtcc aagcagtgca ccagcgaaat cagaagcgcc
    62461 agaagcggca gcaccgaaag aagcagctcc tgcagcagaa agcaaaccag ctgcaccagc
    62521 aaaaccattc aagtccaact taggagactt ggaagaacgt gtagcaatga cgccaacacg
    62581 taaagctatc gctaaagcaa tggtcaacag taagcataca gctccacacg tgacattgca
    62641 tgatgaagta gaagtgtcta aactatggga taatcggaaa cgatttaaag aagttgctgc
    62701 tgcaaatggt acgaaactta ctttcttgcc atatgtcgtg aaagctttaa cagcgactgt
    62761 taagaaatac ccagtattga atgcatctat cgacgatgca aaccaagaaa tcgtttataa
    62821 acattactac aatatcggta ttgcaactga tacggatcac ggactatatg taccaaatgt
    62881 gaaagatgca gaccgcaaag gaatgtttgc tattgcagac gaaatcaatg agaaagcaaa
    62941 attagctcat gacggcaaac tatctgcaga agatatgcgc aatggaacaa ttacgatcag
    63001 caatatcgga tctgtaggtg gcggttggtt tacaccagtc atcaactatc cagaagtagc
    63061 tattttaggt gtaggaacga ttgcacaaca accaatcgta aatgctgaag gcgaaatcgt
    63121 tgtcggtcgt gtgatgaaac tgtcattaag tttcgatcac cgtatcgtag atggagcaac
    63181 tgcgcaacaa gcaatgaata atatcaaacg tcttttagct gatccagagc tattaatgat
    63241 ggaaggatga taaacaaatg gtagttggag attttgctgt agaattagat acagttgtaa
    63301 ttggtgcggg acctggtggc tatgtagctg ccatccgcgc tgctgaaatg ggacaaaaag
    63361 tagcaatcat tgaaagagag tatattggcg gtgtttgttt gaatgtcggc tgtattcctt
    63421 caaaagcact gatagctgca ggacaccatt atcaagagtc actagattct gaaatgtttg
    63481 gagtaactag tgaaaacgtc aaactagatt ttgcaaaaac acaagaatgg aaagaaaaca
    63541 aagttgtcca tacgttgact tctggtgttg gtttcctatt aaaaaaacat aaggttgaaa
    63601 caatcgaagg agaagcgttc ttcgtagatg atcacacact acgcgtcatt catccagatt
    63661 cagctcaaac ttattcgttc aataacgcaa tcatcgcaac aggttcccgt ccaatcgaga
    63721 ttccaggatt taaattcgga ggacgtgtgt tagattctac tggcggatta gcattgaaag
    63781 aagtacctaa gaaatttgtg atcatcggtg gcggtgtgat tggtgccgaa ttgggcggag
    63841 cgtacgcaaa cttaggagca gaagtaacga ttcttgaagg ctcaccacaa attttaccaa
    63901 cttacgaaaa agatctagta aaattagtag aagatgattt caagaaaaaa ggcgtaacag
    63961 tcgtaacgaa tgcaatggcg aaagaagcag tcgataacgg tgacagcgtg acagtgaaat
    64021 atgcagtgga cgggaaagaa gaatccgtta ctgctgatta tgtgatggtc actgtaggtc
    64081 gtcgtccgaa tacagatgat atgggcttag aacaagcggg tgtcgaagta ggagaacgag
    64141 gattgatcac ggtggataaa caaggacgta cgaacgtacc taacatctat gccatcggtg
    64201 atatcgttcc aggggcggca ttagcacaca aagccagtta tgaagcaaaa atcgctgctg
    64261 aagcaatctc tggcaaaaaa gtagctgtag attataaagc aatgccggca gtagccttca
    64321 ctgatcctga attggcttct gtaggtatga cgatcaaaga agcaaaagat gctggtatcg
    64381 aagcaaaagc atataaattc ccattttcag ggaacggtcg ggcattatcg cttggtaaaa
    64441 cagaaggatt catccgtcta gttacgacga tcgaagataa cgtgcttatt ggcgcgcaaa
    64501 tcggtggagt cggcgcaagt gatatggtct ctgaattagc tttagcaatc gaatcaggaa
    64561 tgaatgctga agatatcgcc ttgacgattc atcctcatcc atctcttggc gaaatcgtca
    64621 tggatgcttc ggaattagca ttaggtttac caatccatat ttgatttgaa taatacaggc
    64681 gaaacgcaac tgaaaaatca gttgcgtttt gttttttaga attatcaata atattattat
    64741 gtaaacttaa aaatatgaca acttcttggt ttattaccac ttcctatgct acactgaaga
    64801 ggaatatgaa ataggagagt tgaagatgca agaatatcag tatccacttg atttagattg
    64861 gacaacagac gaaatggtca ttgtaatgaa gatgtgggaa gatctggaac gtgcgaatga
    64921 aaaagggata aacaatgaag cttttttgaa gacttatcag cagtttaaaa ctgttgtcaa
    64981 atccattggt gaggaaaaac gattgggacg ggaatttgaa aaagcttccg gttattcttt
    65041 atatcgtaca gtgcagcaag caaaaaaaaa tccaaacaaa actttgagaa tgaacgggtg
    65101 agataaatgg aacagatgat tgatcatatc aagtcctggc ttgaagaagc tggtgagaaa
    65161 atcaaatctt ctcaacaaac attaacaatc tcgcaaaaat caaatcgaac ggatctagta
    65221 acaaatgtcg atcgaaacat ccagaatttt ctaatcgaaa aaatccattc ctttgatcca
    65281 gaggcgaaaa tattggcaga agaagaaggg tgtaatcaat tatcggatct cgatggaaga
    65341 gtctttatca tcgatcctat tgatggcacc ttgaattttg tgattgaagg ggaaaatttt
    65401 tgtattatgc tcgctgttta tgaatccggc gttggaaagc ttggtttcat ttatgacgtg
    65461 atgagaaatg aacttttttg gggtggaaaa ggaataggcg tgtataaaaa tgatgagcca
    65521 ttgcctagac cagcaaataa atccttatcg gaaggcttac ttggtgtgaa tagttatctt
    65581 ttcggtcata ataaatatgg aatccgagaa attgcggaaa gcagcatggg gacacgtatg
    65641 tacggatgcg ctggtcttga actagtggca atcttgaaag gaaaccacat tgcttatatt
    65701 tcaaacctta gcccttggga ttatgcagcg ggaaatgtct tattggaaga gtttggtatg
    65761 gtaacaagcg gtctttttgg tactcctttg acatttgaaa aaagggaata ctatttagca
    65821 gggacttctt gtgcatatgc agaaatcgca gaccttttga aaaaagaagg ctgaaaaaaa
    65881 ggtagaaatc attcacaaaa atgataaaaa cgagcaaaag attaattttc tcgaaaaaat
    65941 gaaaacattt tactatattt catgaaaaaa gtctgctata attagcagtc gagtaaaatt
    66001 tgccctgtca taaagaacca ataaaaaagg agttagttac attgaattat agaaacgata
    66061 tccgtaacgt cgcaattatt gcccacgttg accacggaaa aacaacatta gttgatgaac
    66121 tattaaaaca atcagataca ttagacgcac atacgcaatt gcaagaacgt gcgatggact
    66181 caaatgcgct agaaaaagaa cgtggtatca cgattttagc caaaaacaca gctgtagact
    66241 ataaaggtat tcgcgtaaac atcatggata ctccaggaca cgcggacttc ggtggcgaag
    66301 tagaacggat catgaaaatg gttgacggcg ttgtgttggt cgtagatgcg tatgaaggaa
    66361 caatgcctca aacacgtttt gtattgaaaa aagcactgga acaacatatc acaccaatcg
    66421 ttgtcgtaaa caagattgac aaaccttctg ctcgtcctga gcatgtcgta gatgaagttc
    66481 ttgaactatt catcgaatta ggagcagatg atgatcagtt agactttcct gtgatttatg
    66541 catctgcact aaatggtacg tcaagcttgt cagatgatcc agcagatcaa gaaccaacca
    66601 tggcacctat ttttgataca attattgaaa aaatccctgc tcctgtcgat aacagcgacg
    66661 aaccattaca attccaagtt tcattacttg actacaacga ttatgtaggt cgtatcggaa
    66721 tcggacgtgt atttagagga acaatcaaag taggagacca agtagcatta attaaattag
    66781 acggaacagt taagaaattc cgtgtaacga aattatttgg tttctttgga ctaaaacgtc
    66841 tagagattca agaagcaaaa gctggagact tgattgctgt ctcaggtatg gaagatatct
    66901 ttgtcggtga aacagttact cctgtggatc atcaagatgc gttacctatc cttcacattg
    66961 acgaaccaac attgcaaatg actttcttag ttaataattc accatttgcc ggtcgagaag
    67021 gtaaattcgt aaccgctcgt aagattgaag aacgtttgat ggcagaactt caaacagatg
    67081 tatccttacg agttgaacca acaaactctc cagatgcatg gacagtttct ggtcgtggag
    67141 aattgcattt gtctatcttg attgaaaaca tgcgccgtga gggatacgaa ttgcaagtat
    67201 ctcgtccaga agttattgaa aaagaaatcg acggtgtaaa atgcgaaccg tttgaacgcg
    67261 tgcaaatcga tacaccagaa gaatatatgg gtagtgttat cgagtccttg agcttacgta
    67321 aaggtgaaat gcaagatatg gtccatacag gaaacggaca aatccgtttg acattcttaa
    67381 cacctgcccg cggtttgatt ggttattcaa ctgaattcct ttcaatgaca cgtggttacg
    67441 gaatcatgaa ccatactttt gaccaatatc tgccaatgct tcctggtcaa attggcggac
    67501 gtcaccaagg ggcgcttgtt tcgatcgata caggaaaagc aacgacttat tctatcatga
    67561 gtatcgaaga acgcggtaca gtcttcgtag aaccaggtac agaagtatat gaaggaatga
    67621 ttatcgggga aaacagccgt gataatgatt tgactgtcaa catcacgaaa gcaaaacaaa
    67681 tgacaaacgt acggtctgct actaaagacc aaacatctgt catcaaaaaa ccaaaacaat
    67741 tgacgttgga agaatctttg gaattcttga atgatgatga atattgcgaa gtaacaccag
    67801 aaagcattcg cttgagaaaa caaattttag agaaaaatgc tcgtgaaaaa gcatctaaaa
    67861 agaaaaaata atctgtaaaa ggatgaagag agaaaccagc ttgttataaa aaatagctgg
    67921 tttctttttt tgtccaactc ttttctgtgg agaagtcatc tttgtcaatg gacataaatt
    67981 cagaaaatac gccgaaagac gtttaaaaga cttttaaaaa acttatttct agagagtttt
    68041 tgtttcataa taagataatg tttcacgaaa ggagatcaac ttgtccttcg aagaaaatcg
    68101 aagtaaaatg gcaagagaaa ggagcgggaa gatgaacgac ttattaaaac aatactttgg
    68161 ttatgatgag tttagacctg gtcaaaaaga aattatccag aaagtcattg atcaggaaaa
    68221 tgtattagga atcatgccta ccggaagtgg gaaatcaatc tgttaccagc ttccagcttt
    68281 actattagat gggcttactg tggttgtgtc ccctttaatc tctttgatga aggatcaggt
    68341 ggacgcggcg aatcaattag gtatacccgc cacttttatc aatagctcgt tagatggtta
    68401 cgaaacagct agacgcttcc aagaaatcga tagacagcag taccgtttac tttatatcgc
    68461 tcctgaacgt tttatcatgc cggattttat ccaagcaatg aagcgatgga acgtccgtat
    68521 gatcgctatt gacgaggcac attgtatctc tcagtggggg catgatttcc gcccaagtta
    68581 tttacagatg gcgaaccagc tagatcaact gccaaatcgt cctgtaattg ttgctttgac
    68641 agctacagcg actgtgcaag tagctgctga cataaaaaga cttttaaaaa ttccagaaaa
    68701 caaccatatt cagactggtt ttgaacgaga aaatctacga tttcaagtaa ttaaagatca
    68761 aaaaaaagaa caatatctaa tcgaatattt aaagatcaac aaaaatcaat ctggtatcat
    68821 ttatgcagca acgagaaaag aagtagaccg tctctatcat ttgcttaaaa aattcgattt
    68881 ttccgtgggt agatatcatg gtggtctaaa tgagaatgag cgaacagaaa tgcaagaagc
    68941 ctttttatac gatcgcttgc agctgattgt agcgacaaat gcatttggta tgggtataaa
    69001 taaaagcaat gtccgttttg ttatccatta tcaaattcct ggctctctag aagcatacta
    69061 tcaggaagct ggtcgggcgg gaagagatgg tctttccagt gaagccatcc tgcttttctc
    69121 tcctcaagat atccaagtcc aaaagttttt tatccagcag tcccaaagag aagaagggca
    69181 aaagcaaaaa gaatatgaaa aattaaaagc gatgacagaa tacgtgtata ttgagtcctg
    69241 tcttcagcaa tacatcctaa actattttgg cgagacaagc agtccgtgca accgttgtgg
    69301 taactgttta gatgacagag aaatagtaga agtaacgaca gaagcacaga tggttctttc
    69361 ttgcttaaaa cgtatgggtg agaactatgg taaacaaatg ctgatgaaag tcttagcagg
    69421 atcgaaagaa caaaagctgc aagctttagg ttttggacat ttatctacct atggactgat
    69481 gaaaaaccaa tcgcaaaaag aaacgatgca gttaattgaa tatttgatat cgaatggcta
    69541 tcttctgacc atcaatggtg agtatcctgt tttgaaagta acagaaagag ggatacaagt
    69601 attgaaagga caagaatctg tttatcgaaa ggaacccaag aaagtccagc agctatcgga
    69661 tgaagaaacc gatacactat ttgaagtgct gcgggaactg cgaacggatc tagcatcaga
    69721 agcaggcgtt cccccgtatg tcgtattttc agattccaca ttgaaagaaa tgagccgaat
    69781 acgtccatca agcagattgg agatgcttca gataaaagga gttggccaaa gcaagcttga
    69841 taaatatggc gaagcctttt tatctcggat aaaaaatgcg gactctaacg ttctggataa
    69901 gtagaaagaa cgaatacgta aaaatgaata aaaacaactg gttaaatctg ttttttttgc
    69961 tccttatgaa aatcaaatat gttataatta aggaattaga atgggaggta agcacagatg
    70021 gattctttgt cgaaaaaact tgcgatccaa gtattaaatg atgaagcaga acggatcaaa
    70081 cgattaatcc gaaatcaaaa caacagtctc tgtatctcac aatgcaaagc cttcgaagaa
    70141 gtggtagata cacagatgta cggcttttcc cggcaagtcg gttttgctgt ccgtatagga
    70201 gtaatcggaa agaacgaggg ccagctgatg ttaagtgagc tagaacgaga attgaatcag
    70261 ttatatagtg aggtttacca agaaatttat gataaaaaag aagttggcaa ggaggaatag
    70321 acttgcctaa gaaagtgaaa aaaaggcatc tattagacta tagcatcttg attccctatt
    70381 taattttatg tgtattggga ttgatcatgg tatatagttc tacgtcgtat ctattattag
    70441 aaaacggaca aaatccgtcc gcatcagtca ttaaccagag tattttttgg gtgcttagct
    70501 tgattgctat cgcactactt tataaaatga aaacagatgt tttgaaaaac cagcgtttga
    70561 tcatggcagc catcgcagtt ttgacgattt tgctgctgat tgttgtgttt tttggtaagg
    70621 agatcaatgg ggcgaaaggt tggctgcaaa ttgcaggatt ctcgatccaa cctgccgaat
    70681 acctaaaaat catttctatc tggtacctgt ctctgacttt atctaaaaga caaaacagtg
    70741 tacaaaaaga ttttcttggg actgtgaaac gtccacttgc aatggttatc ggacttactg
    70801 cattagtcgc tattcttccc gattttggta atgcggcagt tatctttttg attattttgg
    70861 ttctgctttt agccagcgga gtcaattatg tgtatacttt gattgtagga gtaggaggct
    70921 tttgtctaag tacttttacg atctggctga tcaatatcac aaacggaaag atcttacccg
    70981 gacgtttaca gtatatctat aatcgatttg ctatttacca aaatcctttc tcagacgaat
    71041 taaataaagg gcatcaacta gtcaacggct actatgccat gttcaacgga ggactatttg
    71101 gcaggggatt aggtaacagt atccagaaaa aaggattttt acaagaagca caaaccgatt
    71161 ttatttatgc aatcgtagtg gaagaactgg gagtgattat ggggattttg atattagcgt
    71221 tgctattctt tatgattgtg cgcattgtcc ttgtaggtat ccgttcaaaa gatccgttca
    71281 attcgctttt gtgcattggg atcggcgcaa tgtttttgat ccaagtattt gtcaatcttg
    71341 gaggaattac tggcgttatt cctttgacag ggatcacgtt cccattttta agtcaaggag
    71401 gctccagttt gctgatgctt tcgatttgtg tcggctttgt cctcaacatc agtgcggatg
    71461 aaaaacgcaa gagcttaggt ttataataaa aaaagatgta ggagtggttc gatgaaaaaa
    71521 gttttagttg ctaacagggg tgaaatcgct gtacgtgtat ttcgtgcttg tacagaatta
    71581 ggaattaaaa ctgttggaat ctatgcagaa gaagatgaat attccgtaca ccgtttcaaa
    71641 gcggatgaag catatttagt aggacaagga aaaaaaccga ttgatgcgta tctagatatt
    71701 gaagggatca tttctatcgc gaaagaatgc ggagctgacg ctattcatcc gggttacggt
    71761 cttttgtcgg aaaacttgaa ttttgcgcaa cggtgcgaag aagaaggcat catttttgtt
    71821 ggacccaaac tgcatcattt agatattttt ggagataaaa tcaaagcaaa agctgcagct
    71881 atcgaagcgg ggattgcttc gattccaggc acagatggac caatcgcttc tatagacgat
    71941 gccttggagt ttgcgaaaca atacggttat ccgattatga tcaaggcagc tctaggcggc
    72001 ggcggacgcg gcatgcgtgt agctcacgat gaaaagagtg caagagaggg ctacgaacgt
    72061 gcgaaaagcg aagcaaaagc agcctttggt agtgatgaag tgtatgttga aaaatacatc
    72121 gctaatccta agcatatcga agtacagatt ctcggggata cgcatggaaa cgtcatccat
    72181 ctttttgaaa gagattgttc tgttcagcgc cgtcaccaaa aagtagtaga agtagcgcct
    72241 tgtgtctcta tgaatgaaca gcaacgtcaa aaaatctgcc aggcagctgt ccaattgatg
    72301 aaatatgtcg ggtatgtcaa cgcaggtaca gtagaatttc ttgttgaagg agatgatttt
    72361 tactttatcg aagtcaaccc tcgcgtccaa gtggaacata cgatcacaga aatgattaca
    72421 gatattgata tcgtaacaac gcaactgttg attgctcaag gtttggatct gcataaagaa
    72481 atcggattgc ctcagcaaga aggaattaaa ttaaatggtt cagccattca atgccgaatc
    72541 actacagaag acccgttgaa taacttttta ccagacacag ggaaaatcga tacgtatcgt
    72601 tcacctggtg gttttggcgt acgtctggat gtcggaaatg cttacgcggg ctatgtagtg
    72661 acaccgtatt ttgattcatt gctagtaaaa gtatgtacgc atggtgctac ctttgaaaca
    72721 gccattcaga aaatggaacg ttgcttgaaa gaatttagaa tccgtggagt gaaaacaaac
    72781 attccattta tgctcaatgt catcacacat cctgaattcc aatcaggaaa tgcaaaaaca
    72841 accttcattg atagtacagc aacattattt gaatttcctc gactacgaga ccgcggaaac
    72901 aaaacgatga aatatatcgg tgaaattact gtgaatggtt ttccaggtat cgaaagtggc
    72961 gaaaaaccat tttatgaaga accacgtatg cctaaagatc taatcacaag aactgattac
    73021 gtaacggcta agaatgtatt agatgcgaag ggtgccgatg ctttggtcga atggatcaaa
    73081 ggacaggaaa atttgttgtt gacggatacg acatttagag acgcccatca aagtctgcta
    73141 gcaactcgtg ttcggacaaa ggatttcaag cagattgcac gtttgactgg agaaggtctt
    73201 cctgaactct tttcaagtga gatgtggggg ggagcaacat ttgatgtggc ttaccgcttt
    73261 ttgaatgaag atccttggca acggttgaga aaaattcgtt ctttaatgcc taatacgttg
    73321 ctgcaaatgc ttttcagagg ttcaaatgcc gttgggtatt ctaattatcc tgacaatgta
    73381 ttggtggaat ttgtcaaaga agcagcagcg caaggaatcg atgtatttcg aatctttgat
    73441 agtttgaact ggactccaca aatggaaaaa agtatccaag ctgtacggga tactggtaaa
    73501 atcgcagagg cagctatttg ctatactggc gatatcaacg atccaagtcg ggcaaaatac
    73561 aatgttcagt attataaaga catggcaaag gaactagaac aactgggtgc gcatatcatt
    73621 gcaatcaaag atatggctgg gctgcttaaa ccgcaggcag cttatcgttt gattagtgaa
    73681 ttgaaagaaa cgacagatct gccaatccat ctgcatactc atgataccag cgggaatgga
    73741 atcatcactt attctgctgc ttcaaaagca ggagtagaca tcgttgacgt ggcaatgagt
    73801 gcaatgagtg ggaatactag tcagcctagc atgagcagct tgtattatgc actggtcaat
    73861 ggtccacggc tgccagaaat cacgattgaa aatgcacaga aattaaacca ttattgggaa
    73921 gatgtgcgca tgtattacaa accgtttgaa aacggcttga atgctcctga aacagaagta
    73981 tatatgcatg aaatgccagg aggacagtat tcgaatctac agcaacaggc aaaagctgtc
    74041 ggattaggtc atcgctggga tgaaatcaaa caaatgtatc atactgtcaa cttgatgttt
    74101 ggcgatatcg ttaaagtaac tccatcatct aaagtagttg gagatatggc actatttatg
    74161 gtccaaaatg atcttacaga agaagatatt tatgaaaaag gcgaaacact aagtttccct
    74221 gaatctgttg tgactttctt ccaaggagaa ctagggcaac ccgttggcgg atttcctaaa
    74281 aaattgcaaa agattattct taaaggcaga cctgcattga atgaacgtcc aggtcttttg
    74341 gctgaatcag tcgacttcaa cgaagtaaaa aaagaattgg cagaaaaaat tggctatgaa
    74401 ccaaaacaag acgaagtatt aagctatttg atgtatccgc aagtcttttt ggactatcaa
    74461 aaagcatata atcaatttgg cgatgttaca ttactggaca caccaacatt tttccaaggt
    74521 atacgtttgg gtgaaacaat caatgtacaa atcgaacgcg gaaaaacgct tatcattcgt
    74581 ttagatgaaa tcggagaacc cgatatcgaa gggaatcgcg tactgttctt caatttgaat
    74641 ggacaaagac gtgaaatcac tatcaaagat aattcgatca tcagtacggt ccaaacccga
    74701 ttgaaagctg aaccgacaaa ccgagaacaa attggagcaa cgatgtctgg ttctgttttg
    74761 gatgtattag tgaaaaaagg cgataacgtt aaaaaaggtg atacgttgat gattactgaa
    74821 gcgatgaaaa tggaaacggc catcgaagca cgttttgacg gagaagtcgc acatgtatat
    74881 gtgtcttcag gagacactat ttcgtctggt gaccttttga ttgaagtgac tgagaaataa
    74941 taagcgaaag gtggggaaac atgaaaagac tgggtttgtt ttggctgatt ctgctattag
    75001 ttattgtggc attctacatg gaaccagtct tgtttccgcc aaaacttgaa gtgaaggaaa
    75061 actctgatca acaaatatca aaccaaatga caacgtggaa ataccaggag cttaaagcaa
    75121 ctggtttttc tacttatatc ggaaaaccga tcgatcagct ggaaaaggat tttggccaag
    75181 cctatgatcg attaactagc ggatttggtt ttgaaacaag atattatgaa aataaagcta
    75241 gtcatctcac atttgaagcg aacatccaag acagcagagt cacaactgtt aaagtactgc
    75301 aaacagggcc tgctgatatc gcgccgtttt atttagggat gacgatgcaa gatcttacga
    75361 aaatcactac tatctatact aatttcactt ttgagtacca tgatacagaa gttggaatcg
    75421 aactaatgga agaggatatg aattatcgtc cgctaattgc ttttgataac gacacttttg
    75481 ctattttatt ttttgatcaa acaacaaacg gcctattttc agttgtgtat ttagataaag
    75541 atagtctttt gaaactattg ccatatcaag tgtttggtga aggccttcca cattatcaag
    75601 cagataaatc ggctgattgg gaatcaatca atcgcgttaa agaacgaaaa agtacgactt
    75661 tattaaatat gcttagaaga gaagacgaac ttcccatata caatcaaaca cttgcctttc
    75721 aaaaccacag tcagctgcta ctacatgatt ttttgacaag gcctgaggaa atattgtcag
    75781 aggaacgtat ggctgattgg caaaaatcgc taggcagtgc tcaaacgaca gtgaaattct
    75841 cactgacgaa tgatgaattg gagaagttaa tcagtaaaca aaaattagaa aatactagtg
    75901 ggctatttat tcatccggcg attgacgcta ctttttcgtt tttatattgg tacagcgatc
    75961 cttacaacca tgaccgtttc atggacccgg gaacggattc tgtaggcatt gctttttcta
    76021 aagaaaatat gttagtctta ttgcaagaag aaaacggaga aagtagtgat agcagtgatc
    76081 gttaacgaag agctgtttca actggaggat caatgcctcc aagtcgctga aatgataaaa
    76141 aagagtaaaa cgatggcttc ttatcttgaa cataaacagt caatggaaga atctgcaaaa
    76201 gtttgccagt tgaaagaagc gttcttgtct aagaaagaag catttgaaaa tgtagcggca
    76261 tatggtgagt atgcacctga ttatcgtgaa aaaaaacgag ctgttcgaaa agcaaaaaga
    76321 cagcttgata tggatgagca tgttgcagct ttccgatata gtgaaacaca gctgcaaagt
    76381 ctgttggatc aaattgtcca agtacttgcc gaagaagttt ctaatgaaat caaagtagat
    76441 gcaggtagcc cgttttttga aacgaaaaaa cactttggat gcggaggtaa ttgtcatgca
    76501 attagacaat agtgaatttg aaataacaaa acgacgcggg ctgatcgtat gggtctacag
    76561 cttaaaacaa ttaaagaatc tgaaaaagtt cggcttgttg cattatgtct caagaagaat
    76621 gaaatatgtc gtgctttact taaatgaaga gacattggaa gaaacagaag aacgaatccg
    76681 taaattacat tttgttcgaa acgtagaacg ttcttaccga ccggatatcg aaatgaattt
    76741 tgccgaaaaa ataggaaaca aaacagtatt aaaagacgaa ggtttcgaaa ttgaagagct
    76801 aaatacagaa atccgattag ctgaacatct ttaaaaatct cttatgagca gtttttaaat
    76861 aaaaaataac ttggataacg atcgattttt gatcattgtc caagtatttt tttggtatac
    76921 tagtaccagt accatttcta aggagtgagt agatgcggat aatatcagga aattatggcg
    76981 ggcgccgttt aaaaagttta gcaggggcga acaccagacc aactacagat aaagtaaaag
    77041 aatccatatt caatatgatt ggaccatatt ttgatggaga gacagtattg gatctgtttg
    77101 caggaagcgg tggtttagct atcgaagcag tatctagagg ctgcagtcat gcggtatgcg
    77161 tagacaaaaa ttaccaagcg ctaaaaatca tcaaagaaaa catcgaaatt acgaaagaac
    77221 cagagaaatt taccacaata aaacttgatg cggacaaagc aatcgaacgg ctagcagcgg
    77281 agaaacaaca attcgactac ttgtttcttg atccgccata tgcaaaacaa aaaataatcg
    77341 atcaaatcca taaaatggaa gaactgggac ttttttctaa aaccgcagtt atcgtatgcg
    77401 aaacggataa aacagtcgaa ttgccagaaa ctgtggctgg attccatcaa ataagaaaac
    77461 agatttatgg cataacggcc gtaactatct atagaaaaga gggaaatcaa tgaaacgtgc
    77521 attattccca ggaagttttg atccttttac aaaaggtcac ttagatacgg tggaacgtgc
    77581 ggctaaatta ttcgatgagg tagtaattgg tgtttttatc aatacttcaa agaaaagttt
    77641 gtttccccca gaagaaagaa tgacattgat cacaaaagct gtttctcatc ttcctaatgt
    77701 aaaagtcatg catcaagaaa atcaattgac ggtagaaaca gcaaaagaga ttggtgcaga
    77761 tgcgttgatc agaggcatac gaagtatcaa agactttgaa tatgaacgag agattgctca
    77821 gatgaatcat catctatatg atgaattgga aactgtcttc ttattagcaa aaccagaata
    77881 tagtcacgtg agttccagta ttttaaaaga agtactgcac tttggcggag atgtgagcag
    77941 ctacttaccg ccagtgatca acgaagcttt agctggaaag cgtgaatcaa atgaaagcta
    78001 aacaatggtt gaaaacagta ctgattatca tatttggaat gttgatcatt ggatttgtag
    78061 tagttccctt gccgtattat gtagaatcac cgggagccac gatcgatctg aaagaactaa
    78121 ttacggtcaa tgataaggaa gataaaggtt ctgggtcttt ctctcttacc tctgttggta
    78181 tcaggcaagc tacaggtttc ttagctttaa aagctaaaat gacccctttt gaagaattga
    78241 tcagtgaaga agaattgact ggtggggcga caaatgaaga atataatcaa atgcaacagt
    78301 attatatgga ttcttcccaa aatgcagcaa tcgaacaagc gttaaaactt gcagacaagc
    78361 cttataaaat ggaattcaaa ggcgtatatg tcatgagtgt agagaaaaca tcgaatttct
    78421 ttggcaagct ttcagttgga gatacagtaa caaaagtaga cgggaaaagc tttcaaagta
    78481 cagaagcatt tatggattat gtaaaaagcc aaaaagttgg acaaacaatc gaaatcagtt
    78541 atttgcataa tggtgaagaa aaaacagctt ctggtgatct gatcgagtta ccaacagata
    78601 aaaaggcagg aataggtatc acgcttactg accataccga gatcgaatca gatacagatg
    78661 ttcgtattaa ttctggctct atcggtgggc catcagcggg attgatgttc actttagaaa
    78721 tctatgaaca agtcactggt aaaaacttgc gacatggtaa gcaaattgca ggaactggaa
    78781 cgatcaacag tgaaggagaa gttggccgaa tcggaggaat cgataaaaaa gtggcaagtg
    78841 cggataaagc tggcgtagag attttctttg caccagatga tacgatttca gacgatgtta
    78901 agaaagaata tcctgaaata aaaaccaact acgaagaagc aaaagctgcc gctaaaaaat
    78961 tgaattcctc aatgaaaatc gtaccagtaa aaacagtaca agaagcgctt gactatctag
    79021 agaaaatgaa atgaaactaa ataattaaag aggggttgtg acacgagttc tgtcacaacc
    79081 cctctttttc gtcaaaaatc ttcacgataa aaataaaaac aaaagaaaaa attgacgtaa
    79141 ttagatgttg aagggacgat tttgccagag ctcactgttt cttcaatttt aaatgaaagg
    79201 agtaatgaat gatttttact ggtcattctt tgaaaaaaga acaatgatag aaaaaatcag
    79261 caagaaaata ctagcttcac ccaaattgat catttggatg tctatgatag ttcttatctt
    79321 ttgtatcgca agtggttttt tattatcaaa tttgctattt tatagagaaa cagaagagac
    79381 tgctgcaaaa gaaatacttt cttcgtttag ttccagtgga gaagatccat cagctgaagg
    79441 aaacgatgta gaagaggatg ctcgtccaaa ggtcatgtac accgatatca aaggaagtgt
    79501 gaaggagcct ggtatctatt ccttttcttc agaagaacgg gtctatgatg tactaaagcg
    79561 tgcaggtgga ttattagaag aagcggatag cgatcgaatc aacttttctg cgaagataga
    79621 agatcagcaa gtcctatata tccctgctgt aggtgaagag ctgccagaac atcttgatca
    79681 gttggcatcc tcggaaggaa aacagcctac agcagattcc gagcctccca aaataaatat
    79741 caatacagcc actccatcag aacttcagca gattcctggg atcggatcag tcaaagcgca
    79801 agaaatcatt cgatttagag aagaaaacgg atccttccag aaagtggaag acctgcagga
    79861 gatttctggt atcggtgaaa agacagttga aaaactaaaa aacttcgtta caattaaata
    79921 gaatgattca aaatcgactg gcacgagcaa taaatggacg ctaatggata ctaagaataa
    79981 tccaattcta agagcttggc aggacgagga ggtagtattt ttggatagga aatagcttga
    80041 ttgttcgcaa agaaacatta aaaaaaccaa tgctgaatgt ctatctccaa aataaaatat
    80101 ctgggattca tattatgaat acggctgtca gcggcaataa cagtcaagct ctacgagaac
    80161 gctttgccaa agatgtactt agctatacag cagataaagt atttattctc attgggacga
    80221 atgatctagc agagcataaa caactttcta aagaaacgta ccaaaaaatc tgcagtggat
    80281 gatagttcag ctgaaaaaag actatagttc tgatcatgct ttctttatca cgccaccagc
    80341 agtcgatgag acaaaacaaa aatatcggaa taacaagttg atcaaagaat atatctctgt
    80401 tctcacagaa gtagcaaaac gccatgattg tgaagtatcg atttttatac ggaatttctt
    80461 gaacaaatgg attcgaagaa tttttataat gggctaatgg atgacggact tcatttcggc
    80521 agtgaagggt aggatctttt gtcagacagg atgattcgtt atatttaaag aatattttgt
    80581 ttaagcaatg agtacttttt aatcaacttg aaaaaacgga ggaatataaa acatgttaga
    80641 aaaatcttca aaagcatgcg atacgaataa agaaaaagtt actgataagt cacataatga
    80701 acgcattcct tgggaccagt actttatggc tcaggcagta ctgttatctt tacgtagtac
    80761 gtgtacacgt ttagaagtag gagcgacact cgttcgagaa aaaaggatca ttgctggcgg
    80821 ctataatgga gcagtcagtg gagatgtcca ctgcatagat gaagggtgtt acgtcgtgga
    80881 tggacactgc ttgcgaacga tccatgcaga gatgaatgca ctcctacagt gtgcaaaact
    80941 tggtatcccg acggaaggtt cagaaatcta tgtcacacat tttccatgtt tggcctgtac
    81001 aaaggctttg ttgcaagctg gtgtgaaagg gatcaattat ctccatgact atcgaaatga
    81061 tccttatgcc caagcgttga tcgaacaatt aggtgtatct gtacaccaag ttgctttaga
    81121 ctcacagcat ttttcaaaac ttcaaaatga attaacagga aaacatgtct agcgttttag
    81181 aaaggcatgt tcgaaatcgg ttgatttttc cggcaattac gtcgatgatt atctgtttgt
    81241 tgtattacac tgacaattgg ggttatcgtt tgcttcttgc cggaattttt ttatggtttt
    81301 cgctaaaaga aggacgtttt atcgcgtact tgtgtatttt gtgtagcata acggtatcca
    81361 tttcttatag gctacaagag tattcatttt tagaggaaaa aacaacatct gcgcaaatta
    81421 ctgtatttac agatacgatt cgtgcaaatg gtaatttttt gacaatggaa ggaaggttat
    81481 cagagaataa acaaaagctc cagctttctt atcaagtaaa cacagaagca gaattgaaaa
    81541 catggcttaa cttccgagga aagggaatag aattgactgc tgaaggcgag ttcatctctc
    81601 cagcaaaaaa gcggaatctt tataattttg accaagaagc ttacttcagg aaccatagaa
    81661 tttctggacg gttccaaatt caaaagattg aacagatcac catgcaaaat cactggcgaa
    81721 actggcttca gagaaaaaga ggatctttta tctcctttgt ccaatctgaa tttccgcaaa
    81781 aaacaagtgt atatattaat tctttattat ggggctataa agatgcagga ttcagagaac
    81841 cagaagaaat ttttagggag agtggtttgc tgcatctgtt cagtctatcg ggtctacaca
    81901 tccaatttta tttagggtgg ctgtattatt tatttcgacg tatcggatgg ccactacacg
    81961 tcagtattat tccgttgagt ctactaacta tctgttatgt ttctattgca ggaagttcta
    82021 tttctgtttt gcgtgctagt ttgctgtttt tattgcgatt gctagtcaaa ttgctgaata
    82081 agcattattc caccatggac ttatttgcag ttgtcttttg gcttctttta ctatttgatc
    82141 ctcgatttct ttttcatgcg ggtggacagc ttagtttttt catgagtttt ttgtttatcc
    82201 tgatttcttt tgatggagat tggttgaaaa aaacggcctc tcacgtcctg tttactatga
    82261 tcacgatgcc gttgattgta tggcattttt atgagtggcc tattttaggt agtcttttta
    82321 cattactgat tgcaccggta ttcaagtttc tttttcttcc tggtgtgtta tttctcacac
    82381 tcttcaatcg ctggctgccg gaaactttac ttttgctgat agaagaggga ttaatcttat
    82441 ttgagcgaat aatcgatttt tcttcagggt cgtctctcat tataggacgt ttgccccttg
    82501 tgctatctgg gatgtatatt ttcggattac tgatttgtat ggataggttg aaagaacaac
    82561 ctgtgaaaca cttgttttat gttggtcttt tttcttttat ttttttgact ttgccatttt
    82621 tacgcttttc ttcaacgatc gcttttatcg atgtcggtca aggagacagt atctttttgc
    82681 aaagtccttt tcgaaaagaa acgattttga tcgatactgg aggtcagtta cactttgaaa
    82741 gagaaaagtg ggcgcaaggt atgatacgtc ctcctgctga aagaaatatc gttccatatc
    82801 taaaaggtca ggggataagt gtaatcgata aactcatctt gacacacgac gatacggatc
    82861 atgtgggaga attacccacg ttatctcagc attttactat caaaaaaatt tatatcggtt
    82921 ggggcgctgg acgaaatgag ccattacaaa ggcatttatc tgaagctcaa aaaaatggta
    82981 ctgagatcat tgaaatcaaa catggggaca ggatcagcgg ttattatgat ctttatgtat
    83041 tgactccttt tgaaaaggga gagggaagaa atgaagattc gattggtcta tggatggaat
    83101 ataacaatag gcgcttttta tttttaggag atttgaatca agcaatggaa aaacaattgc
    83161 ttttgattta tcctaatctg aaagccgatg tagtcaaatt aggtcatcac ggtagccgta
    83221 cgtcaagtaa cacagatttt ctttcccata ttgaagcaaa acatgggatt atatcctgcg
    83281 gcgtaaataa tcgttatggt cacccgcaca gcgaagtatt ggagacacta gaagaaaatc
    83341 agataaaaac atggcgaacg gatcaacaag gaatgatcac ctatcgctgg catccagttt
    83401 ttcatcctca tgggctagtg gaaacaatga tagactagta attctcatct gctcatggta
    83461 ggataatttt tgaaagtggt gagtaatttg agtatacaag cagagttaca aaaaatacgt
    83521 aaagaaaaac ttgcatcttg ttacttgatt ttaggaacag aaaagtttct ccaagatcaa
    83581 gttcggacag aaattgtcaa aaaaatccaa ttagagggcg aagatgactt aaatttttta
    83641 agttttgaca tggaaaatgc aagtttagat gaggtagtag ctgaagcaga gacgttgccg
    83701 tttttcggag atcaacggtt agtattcgta gagaatcctt attttttaac aggtgaaaaa
    83761 gtaaacaatg gaatcgaaca aaatactgat ttattgacgg cttatttgaa agaaccatta
    83821 gaatcgactg tattagtatt ctttgcgcca tatgaaaagt tggatgaacg gaaaaaagta
    83881 acgaaacaat tgaaaaaaac agcaattatc atcaatgtcc aacagttgaa tgaaaaagaa
    83941 gtacgtcaat atctgatgaa taccttagag aattcctcaa tcaaaatgga acgaagtgca
    84001 attgatttat tcttgaggtt gaccgactta gatttatcca aaatgatgag agagttacag
    84061 aaattggtcc tttatggaca aaatcaacag caaatcacgg taagagaagt agaagaatta
    84121 gttcctaaaa cgttagaaca taatattttt gacatgaccc aatatatttt aaaagggaag
    84181 acggaacaag ctttgcgttt atacgaagat ttagttatgc aaggggaaga aacaatcaaa
    84241 attaatgcga ttttgctttc tcaattacgt ttatttttac agacaaaatt tcttgtaaag
    84301 attggttatc agcaggcaaa tattgctgaa acattgaaaa tccatccata tcgtgtaaaa
    84361 cttgccatgc aggaagtaaa aaaattcgat gaacatttgc tggtacagtt gtttgatcaa
    84421 ttagttgaga tggattatca aatcaaaacg ggacaaatcg aaaaagaatt aagttttcaa
    84481 ctattcgttt tgcgcacagg gcagttgtta agaaatcgat aagaagaaag acgagggcaa
    84541 agcatgcggg aaggtcgttt gtttttgtcc tttttttgac aaataaaaaa atcggccgaa
    84601 gccgattctt ttatttagcg atttttttgc ttaaacgaga tttgtcgcgg ttagctttgt
    84661 ttttgtggat tagacctttt gattcagcca tatcaatagc tttaacagct tctttatata
    84721 acgcatctac gttgtcagca cctgaagcta cagcatcttc aaattttttg attgcagtac
    84781 gcatagcgct tgtttgagat gaatttttca cattagcgtt ttcgcttgta cgaacacgtt
    84841 tgattgcaga ttcaatgttt ggcattagtt tcacctccga taacttaagt tcttacgcgt
    84901 gttaccgcat aattcaacat tcattattat acctaaaggc agcgtgcttt gcaatatgaa
    84961 catgacaagt ttttttcctt aacagtcttt tattaggatt ttttttcagt aaaaacggat
    85021 agtacaagct gaaaaaaatc aaaaagacgc tattgtggga ggcaatagcg tcaaaccaaa
    85081 ctagattttt atttatctat ttaaacaaag tttgaatggg aatgtttggt actaagttat
    85141 tatataacaa agagaaccgt ttgcaaagtt ttttttgaaa agggatacga ttccctgttg
    85201 ttctttgttt ttctattaag aaactgcagt tattttataa ccagtatcct ttgatcagta
    85261 aaggctattc tggtgtaccg attattttaa tgtattcatc gatatttttt atcaacattt
    85321 tttttatgac caactggttc cgacggttcc atttgccact tacttccagc acatatttat
    85381 ttaggggcaa acgtaagata tattttgcca aatagcgatc tttaagcagg ccattgatca
    85441 cacggtcctc aagctgcaag ttgatcccag tcagcatcat tggctgttct tgtaaaacgt
    85501 acgtatcaat taaaagacct tcaaaagttt ccacaagcgt cttcctttct tttcaaagta
    85561 acctgaaaac aggcactaat tcttccattt tgatattcgt tgaagtggat gcgatcattc
    85621 tttcttttgt ccactgtaat ctagtataac aaaaaagagc agaagagcca aaaagaaaaa
    85681 agagcatcga attctatagg aagtctcatt ggcacggcct aaattattac aaaacattga
    85741 ttaaaaaaag cccctgcatc gcaggagcat cccctatatc gttttaatag gtcaatcaaa
    85801 aagcagtatc aaacacattt tgtatcaaat caggaaataa tagataagct aagcccagaa
    85861 tcaaaagtac cgctagaaga aaccagccga ttgttcctat gaaatacttt aatttttcga
    85921 acattgtatc acctcgttgt cagtatatca cggatcagta aattgggggt gttaagaatc
    85981 tcctaaaata gacaaaattt gttctatttt cctaaagcaa atacttgtaa gatacagtaa
    86041 gtgataccag aagcaatgac cggtccggcg gcgattcctc ttaagaagac aacacctaaa
    86101 atcgtgccga aaacaagtgc aacagtgatt tgtggatcag aagcaatcaa tcctacaccc
    86161 ttggaagata agacagccac tagcccaccg caaaaaacag ctacccagcc aataggggat
    86221 ttaaaggctt ctaatagatc acgtagaccg atttctcctg ttgcgatagg gattagaata
    86281 gccgctgtga tgatcgtcac cccaatattg attcctttgc cttgaagcca agggaaaaag
    86341 gaacgggagg ctggtatgag cttcataatc aaaacaaaag ctgccgctat catcagactt
    86401 tgatttttag ctaaaattgc tacaagtaaa ataattccta aaaaaagcca actttccatg
    86461 aaatatattc ctcactttct tcgaaacaat cataacatgt ttttagaaag ttacaaaagt
    86521 aggaattcat ccttcaagac ctattcataa gctgtttttt tcataaaata aacaaaatca
    86581 taaaaaaaca ctttgcttat ttgacagaag acagccgcgc tttgtatagt ggacaagact
    86641 tacatttttt tgaagaatca gaaagatcag gtaaaggtct tagtaaacgc aagccattca
    86701 gaataaccaa tattgtgctt ccttcgtgtc ctacaactcc aagaggtaga tttaaaaatt
    86761 gcaggaagtt agaagcaatc aacaagagaa taacaagaat cgaaaaaata atattttgtt
    86821 tgatgatccg ccgcattttt aaagatagcc gatgactgta gacaaattta tttaagtcgc
    86881 ttttcattaa gacgatgtct gcgatatcca tagcggcatc tgtcccttgc cccatagcga
    86941 cacctaaaga agcggctgct aaagcaggcg catcattgac tccatcacca accatcgcat
    87001 ttacttggta aagttcattc tcttttttga ctagttgtgt tttttcttcc ggagtacagc
    87061 ttgcatagta atgttcaatc cctacttgtt cagcaatggc ctttgctgtg ccagaatggt
    87121 cgccggttat catacttgta tggatgtttt gggatttaaa gtaggaaatt acttgttggg
    87181 tatttgcttt tggaatatct agcagaccta aaacagcaat taactgctga tctttagaaa
    87241 gataaatcac ggttttgcct tgattttcca aacgttcgat catttccgca atctctttgc
    87301 tgattatcat cgtctcagga tcatatgcgt gcttgccaac tttccattgc gcacctaaat
    87361 aggtagtttg taatccaaaa ccagtaattt cttctattgg taagtattgg atttcttcag
    87421 gtattgtgca atcgaaatat tgcaaaatcg catgagcaag tggatgcgtc gttttttgtt
    87481 ccatagcaac aagaagttgt tgtgccaaat ccttttcatc aaagaaaaag ctgtcagtta
    87541 caactggttt tcctttagtg agcgttcctg ttttatcaaa ggcaatcgct tttaattcag
    87601 aaagcttttc taaatggata ccgcctttga ttaaaatccc attttttgca gaatttgaaa
    87661 gagcagctag agaagctggt gtagcagatg cgacaagcgc acaaggagaa gccacaacta
    87721 ataataccat tcctcgataa aagctttcat cccaactcca acctaggaaa aagtggggga
    87781 gaaggatcat tagaggaaca gcaagcaaaa tcaattttac ataattattt tcgattcgtt
    87841 cgataaatga agctgtttga gttggcgtat tctgcgcttc ttctactaat tggataatct
    87901 tagcaaagac agtttcggaa ctcgtttttg ttactcgaat cgtgatggga tttccttgat
    87961 tcagcgttcc tgcatacatc tcatcgccta aatatttttc tacaggcaca gattcaccgc
    88021 ttaatgcaga ctcatcgatc gttgcctgtg tgctttctaa aaagccatct atcggaacag
    88081 ctgctccttt agcgacaaat accaagttat ccacctttag ttgagaaaca tctacttctt
    88141 gcatttgtcc attttctttt aataattggg ctttttgtgg ttgaagattc atcaaagcag
    88201 taatttcttt ttgactttta ttagttgtgt attcctctaa tgcaccgctt aaacaaaaga
    88261 tgaatgtcaa cattgctcct tcaaagtaat taccgatcaa acaggcacca atagcagcta
    88321 aagccatcaa taaatccaca ttcagtgtcc gatcattcca caattctttc aagccttccg
    88381 atgtttgttt atatccaccg atgataatgg cactgatata aaaaagtggg gtgatggtaa
    88441 gagagaatcg gtcgaagata aaaccgaaga tcataaataa aaggcagccg attgttgctg
    88501 caaaagcttt tttctgatta gtcatcgtta ttagacctcc ttgtcaaaac aaggatagca
    88561 taataaaaat gtaattgata ataataacag ctaaatgaga acgataatgg ttatcatttg
    88621 gtgattaagc ttgatgttgt ttttattatt tataatgatt attattaaag aaaataaaaa
    88681 accagaacat ttctcaaata agag
//
LOCUS       DACDXO010000005        74844 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E23605_2017
            SAMN12639989-rid9881463.denovo.005, whole genome shotgun sequence.
ACCESSION   DACDXO010000005 DACDXO010000000
VERSION     DACDXO010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639989
            Sequence Read Archive: SRR10023389
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 74844)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 74844)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741914.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:42:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,972
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 33 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 17 of 73
            Pseudo Genes (multiple problems)  :: 10 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..74844
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639989-rid9881463.denovo.005"
                     /isolate="E23605_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(70..690)
                     /locus_tag="IWM35_000442"
     CDS             complement(70..690)
                     /locus_tag="IWM35_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HAP9918323.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
     gene            complement(766..1203)
                     /locus_tag="IWM35_000443"
     CDS             complement(766..1203)
                     /locus_tag="IWM35_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HAP9918324.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTGGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            1366..2196
                     /locus_tag="IWM35_000444"
     CDS             1366..2196
                     /locus_tag="IWM35_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HAP9918325.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLKDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            2180..3547
                     /locus_tag="IWM35_000445"
     CDS             2180..3547
                     /locus_tag="IWM35_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP9918326.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            3566..4375
                     /gene="yidA"
                     /locus_tag="IWM35_000446"
     CDS             3566..4375
                     /gene="yidA"
                     /locus_tag="IWM35_000446"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HAP9918327.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(4418..4861)
                     /locus_tag="IWM35_000447"
     CDS             complement(4418..4861)
                     /locus_tag="IWM35_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HAP9918328.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            4995..5366
                     /locus_tag="IWM35_000448"
     CDS             4995..5366
                     /locus_tag="IWM35_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HAP9918329.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            complement(5535..6317)
                     /locus_tag="IWM35_000449"
     CDS             complement(5535..6317)
                     /locus_tag="IWM35_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HAP9918330.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
     gene            complement(6314..7285)
                     /locus_tag="IWM35_000450"
     CDS             complement(6314..7285)
                     /locus_tag="IWM35_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HAP9918331.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            7377..8771
                     /locus_tag="IWM35_000451"
     CDS             7377..8771
                     /locus_tag="IWM35_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HAP9918332.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRAQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
     gene            complement(8963..10318)
                     /locus_tag="IWM35_000452"
     CDS             complement(8963..10318)
                     /locus_tag="IWM35_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk family potassium uptake protein"
                     /protein_id="HAP9918333.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
     gene            complement(10348..10983)
                     /locus_tag="IWM35_000453"
     CDS             complement(10348..10983)
                     /locus_tag="IWM35_000453"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HAP9918334.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            complement(10990..12366)
                     /locus_tag="IWM35_000454"
     CDS             complement(10990..12366)
                     /locus_tag="IWM35_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HAP9918335.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            complement(12359..14140)
                     /locus_tag="IWM35_000455"
     CDS             complement(12359..14140)
                     /locus_tag="IWM35_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HAP9918336.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            complement(14162..14473)
                     /locus_tag="IWM35_000456"
     CDS             complement(14162..14473)
                     /locus_tag="IWM35_000456"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HAP9918337.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            complement(14463..15449)
                     /locus_tag="IWM35_000457"
     CDS             complement(14463..15449)
                     /locus_tag="IWM35_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HAP9918338.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNLDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFEPETIRKRVRQVYDA"
     gene            complement(15464..16048)
                     /locus_tag="IWM35_000458"
     CDS             complement(15464..16048)
                     /locus_tag="IWM35_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HAP9918339.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQIETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAIAEMENWDESEQ
                     IQFIKNSLYSLPLTGKVTFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            complement(16069..16539)
                     /locus_tag="IWM35_000459"
     CDS             complement(16069..16539)
                     /locus_tag="IWM35_000459"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HAP9918340.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            complement(16536..18560)
                     /locus_tag="IWM35_000460"
     CDS             complement(16536..18560)
                     /locus_tag="IWM35_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HAP9918341.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFMNKLEEVLDLKEKWEQLTEKRDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAAIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKVKERFIHATYLIVLQGWVDAEEKQNLMHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLIEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLFVLPRGMKRFVKFFEILSVPSIIWGLIYSSFFGQALPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALIGVMLIKNTAFVYLGAGLSIIAALCILIVPIIQSPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKTINNWRIFIQ"
     gene            complement(18547..18879)
                     /locus_tag="IWM35_000461"
     CDS             complement(18547..18879)
                     /locus_tag="IWM35_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9918342.1"
                     /translation="MKQVLEALEQIRKAEEENEKRARTLRQQLKTYAEEKEQELEIIQ
                     TNSRQQLKQLVEEKEQFEKQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            19216..19935
                     /locus_tag="IWM35_000462"
     CDS             19216..19935
                     /locus_tag="IWM35_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HAP9918343.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIEKIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFATHEVKIVSD
                     SLLSDLLPDSYFVNSYHHQAVKDLSPLLKAIAFSNDGLIEAVQSKDNMHKILGVQWHP
                     ELSHRVDSSEQRLFDFFVNEF"
     gene            complement(20234..22132)
                     /locus_tag="IWM35_000463"
     CDS             complement(20234..22132)
                     /locus_tag="IWM35_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HAP9918344.1"
                     /translation="MDRYLDLLSEKFPTTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETEISQFTIFTAYPEYVLTTDWYKKQDKS
                     QLIHRLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFTP
                     KNRKDLSEREQEELEKIHQALSILQFKSEHQLLNRRPEFKMDDRKLLEKINYEESTIV
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTANEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDAKEEGSAIINGHTPVKVKKGESPIKANGKAFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRNTTKGMELQQQITQLQR
                     LLDYRKDD"
     gene            complement(22295..23557)
                     /locus_tag="IWM35_000464"
     CDS             complement(22295..23557)
                     /locus_tag="IWM35_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9918345.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPVRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            complement(23550..24449)
                     /locus_tag="IWM35_000465"
     CDS             complement(23550..24449)
                     /locus_tag="IWM35_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9918346.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            complement(24594..26372)
                     /gene="uvrC"
                     /locus_tag="IWM35_000466"
     CDS             complement(24594..26372)
                     /gene="uvrC"
                     /locus_tag="IWM35_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HAP9918347.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKKFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTRQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELANKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMAKNDKHKTNELLFGIDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIEGLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            complement(26554..26868)
                     /gene="trxA"
                     /locus_tag="IWM35_000467"
     CDS             complement(26554..26868)
                     /gene="trxA"
                     /locus_tag="IWM35_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HAP9918348.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            complement(26955..29315)
                     /locus_tag="IWM35_000468"
     CDS             complement(26955..29315)
                     /locus_tag="IWM35_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HAP9918349.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEKQTIT
                     NWLQETEDGLKVQRLRGGIPIPKLENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLSDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNAYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            complement(29430..29977)
                     /locus_tag="IWM35_000469"
                     /pseudo
     CDS             complement(29430..29977)
                     /locus_tag="IWM35_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
     gene            complement(30035..30472)
                     /gene="zapA"
                     /locus_tag="IWM35_000470"
     CDS             complement(30035..30472)
                     /gene="zapA"
                     /locus_tag="IWM35_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HAP9918350.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            30674..31591
                     /locus_tag="IWM35_000471"
     CDS             30674..31591
                     /locus_tag="IWM35_000471"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HAP9918351.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELHKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            complement(31847..32758)
                     /locus_tag="IWM35_000472"
     CDS             complement(31847..32758)
                     /locus_tag="IWM35_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP9918352.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(33161..33739)
                     /locus_tag="IWM35_000473"
     CDS             complement(33161..33739)
                     /locus_tag="IWM35_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP9918353.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            complement(33759..34427)
                     /locus_tag="IWM35_000474"
     CDS             complement(33759..34427)
                     /locus_tag="IWM35_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9918354.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEAKELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            complement(34442..35512)
                     /locus_tag="IWM35_000475"
     CDS             complement(34442..35512)
                     /locus_tag="IWM35_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9918355.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAVGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            complement(35683..37047)
                     /locus_tag="IWM35_000476"
     CDS             complement(35683..37047)
                     /locus_tag="IWM35_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP9918356.1"
                     /translation="MKIVIIGASHAGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQAIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEHTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            complement(37044..38603)
                     /locus_tag="IWM35_000477"
     CDS             complement(37044..38603)
                     /locus_tag="IWM35_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9918357.1"
                     /translation="MRRKLLLLKSEMLGGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     ISVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQICYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFTITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            complement(38659..41139)
                     /locus_tag="IWM35_000478"
     CDS             complement(38659..41139)
                     /locus_tag="IWM35_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9918358.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLADIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISTSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSVQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYEAKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWELTAGPTS"
     gene            complement(41123..41458)
                     /locus_tag="IWM35_000479"
     CDS             complement(41123..41458)
                     /locus_tag="IWM35_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9918359.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPVPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEEKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            complement(41472..42506)
                     /locus_tag="IWM35_000480"
     CDS             complement(41472..42506)
                     /locus_tag="IWM35_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HAP9918360.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTIPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNEFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            complement(42596..43276)
                     /locus_tag="IWM35_000481"
     CDS             complement(42596..43276)
                     /locus_tag="IWM35_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAP9918361.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGSLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
     gene            complement(43560..44663)
                     /locus_tag="IWM35_000482"
     CDS             complement(43560..44663)
                     /locus_tag="IWM35_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
                     /protein_id="HAP9918362.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHRLIIPHVLRDVELPDTTTHFDEEMLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDNGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
     gene            complement(45292..45762)
                     /locus_tag="IWM35_000483"
     CDS             complement(45292..45762)
                     /locus_tag="IWM35_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HAP9918363.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLDSNQAIISELTV
                     GGLVDSLALAEGFTGEDIYEMFSEVRQLKKGEKCIEVHHESPNVRFKRGDNEMIFKNH
                     VSDHTELYEIVNQILSGIFELGPYEFKEENGNPKLNEEWQETIETQRNNPHLFQ"
     gene            46092..47732
                     /locus_tag="IWM35_000484"
     CDS             46092..47732
                     /locus_tag="IWM35_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HAP9918364.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAAFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAAAILEKYEDQITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQIILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     EGLTPGQIPFARKRSDFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQNLNREEITDIKEAVESAKWVPEYKSLED"
     gene            47886..49208
                     /locus_tag="IWM35_000485"
     CDS             47886..49208
                     /locus_tag="IWM35_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HAP9918365.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFWDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRTVKPSIPQLGVGLMVSL
                     TFFILGQIGNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(49276..49710)
                     /locus_tag="IWM35_000486"
     CDS             complement(49276..49710)
                     /locus_tag="IWM35_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9918366.1"
                     /translation="MLREMRLEDAEALREINADQLGYDIPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKYAEKRGIGKALMTGVEEE
                     AKKRGITTIRLNSAEYRVEAHQFYEHIGYHSDKMQKRFLKIL"
     gene            complement(49775..50612)
                     /locus_tag="IWM35_000487"
                     /pseudo
     CDS             complement(49775..50612)
                     /locus_tag="IWM35_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325086.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
     gene            complement(51181..51486)
                     /locus_tag="IWM35_000488"
     CDS             complement(51181..51486)
                     /locus_tag="IWM35_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HAP9918367.1"
                     /translation="MTEHNHSHDHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEEE"
     gene            complement(51511..51942)
                     /gene="ruvX"
                     /locus_tag="IWM35_000489"
     CDS             complement(51511..51942)
                     /gene="ruvX"
                     /locus_tag="IWM35_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HAP9918368.1"
                     /translation="MRVMGLDVGSKTVGVAISDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            complement(51939..52205)
                     /locus_tag="IWM35_000490"
     CDS             complement(51939..52205)
                     /locus_tag="IWM35_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HAP9918369.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPAYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            complement(52460..53185)
                     /locus_tag="IWM35_000491"
     CDS             complement(52460..53185)
                     /locus_tag="IWM35_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HAP9918370.1"
                     /translation="MSKKTTKAIVGIVAAVIAGAFGVSTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            complement(53546..53616)
                     /locus_tag="IWM35_000492"
     tRNA            complement(53546..53616)
                     /locus_tag="IWM35_000492"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(53582..53584),aa:Gly,seq:ccc)
     gene            53733..54485
                     /locus_tag="IWM35_000493"
     CDS             53733..54485
                     /locus_tag="IWM35_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HAP9918371.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            54501..54980
                     /locus_tag="IWM35_000494"
     CDS             54501..54980
                     /locus_tag="IWM35_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HAP9918372.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(55231..56811)
                     /locus_tag="IWM35_000495"
     CDS             complement(55231..56811)
                     /locus_tag="IWM35_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HAP9918373.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(56804..57547)
                     /locus_tag="IWM35_000496"
     CDS             complement(56804..57547)
                     /locus_tag="IWM35_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9918374.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            complement(57723..58499)
                     /locus_tag="IWM35_000497"
     CDS             complement(57723..58499)
                     /locus_tag="IWM35_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9918375.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHY
                     DEILYLMSMPGYKNKIGLVLKDEEILVNGTIKKYKKSLEYEYLCKDLKAYIINTENIT
                     SISRVNEIVTFANGEQLEMGKRSIDKLKNFMQEKIENESF"
     gene            complement(58747..59583)
                     /locus_tag="IWM35_000498"
     CDS             complement(58747..59583)
                     /locus_tag="IWM35_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HAP9918376.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            complement(59594..60523)
                     /locus_tag="IWM35_000499"
     CDS             complement(59594..60523)
                     /locus_tag="IWM35_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HAP9918377.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            60715..61068
                     /locus_tag="IWM35_000500"
     CDS             60715..61068
                     /locus_tag="IWM35_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HAP9918378.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            complement(61142..61798)
                     /locus_tag="IWM35_000501"
     CDS             complement(61142..61798)
                     /locus_tag="IWM35_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HAP9918379.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            62024..63958
                     /locus_tag="IWM35_000502"
     CDS             62024..63958
                     /locus_tag="IWM35_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAP9918380.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            complement(64044..65267)
                     /locus_tag="IWM35_000503"
     CDS             complement(64044..65267)
                     /locus_tag="IWM35_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronyl hydrolase"
                     /protein_id="HAP9918381.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(65261..67159)
                     /locus_tag="IWM35_000504"
     CDS             complement(65261..67159)
                     /locus_tag="IWM35_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HAP9918382.1"
                     /translation="MVVKRGENTMKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQ
                     IDYIHQGVGYGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYW
                     GEIHERDQRMVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQF
                     FKIIVGTIIQKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYG
                     LIYSVLEPEKPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAML
                     WADLLPFKLGEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLW
                     LNKIFLLLALPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPN
                     FHTLTNEKYLKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKT
                     YGQFIVRNNVRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLY
                     ETGFAIECSPEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLM
                     TWENTFIPGLEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKN
                     NKKRLKLC"
     gene            complement(67153..68271)
                     /locus_tag="IWM35_000505"
     CDS             complement(67153..68271)
                     /locus_tag="IWM35_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 88"
                     /protein_id="HAP9918383.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            complement(68354..69187)
                     /locus_tag="IWM35_000506"
     CDS             complement(68354..69187)
                     /locus_tag="IWM35_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP9918384.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVLATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            complement(69180..70097)
                     /locus_tag="IWM35_000507"
     CDS             complement(69180..70097)
                     /locus_tag="IWM35_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP9918385.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            complement(70120..70299)
                     /locus_tag="IWM35_000508"
     CDS             complement(70120..70299)
                     /locus_tag="IWM35_000508"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9918386.1"
                     /translation="MNKINENTSNTVLPATYSDATSYLTTAAYNYWQTGDFENSYLKE
                     AENFAHKYGVKLTGD"
     gene            complement(70324..71448)
                     /locus_tag="IWM35_000509"
     CDS             complement(70324..71448)
                     /locus_tag="IWM35_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP9918387.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
     gene            71631..72539
                     /locus_tag="IWM35_000510"
     CDS             71631..72539
                     /locus_tag="IWM35_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9918388.1"
                     /translation="MVLLLTNNQSSNLVKKTLTYSIDTHLHLSAAPYFRLLINESANL
                     YAEFAHKSIELDNPSLLFIDKQQEVQIFSTQKETQTFIIIQFYFDEQLLAHITGQSFS
                     IDQEITSIITFLKNEQFKIDYLKHSKPAISYDQEINSFFILCQQMSIHLLSLLVTYCL
                     QKIQLSSLPRHYQQELALLTIPSNEKVIPKKKHILYSDELVDPIIRYLEEHLSENFLL
                     EQLAQIFFISPASLKKHFRKVNGKSVISYFKELKMEKAKRLVLENELTYTEIADILGF
                     HSIHHFSSAFKKYTGLSPSKYYLSLK"
     gene            72680..74293
                     /locus_tag="IWM35_000511"
     CDS             72680..74293
                     /locus_tag="IWM35_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-disulfide reductase"
                     /protein_id="HAP9918389.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
BASE COUNT        22190 a        15419 c        12818 g        24417 t
ORIGIN      
        1 atcgtgtcac acctccttat tctgataggc tgatcgtctc tgtcacaacc tctcgtaaga
       61 aaataatgtt tattcttttt cagagtcaga aaattcttcc tcttcataat cttcatcatc
      121 atcgtcttcg tctatgatac ttaaatcttc ttcgatattt cctggcaagt cttcttcatc
      181 atctgtcggg tcatctgctc cgatttcttg caagtcagaa ttataagcag cgatttcttc
      241 atcttcatca tcatcgtcat caaacagatc atcgtcatct tcttctgtca gatctgtatc
      301 ttctgggtca tcgtcattgt aatcgatcgc gtcttcatca tcattgataa aggcgttgac
      361 tttcttgcgt ttacggcgac gtggagtatc ttcatcctct tcgtccattc catggtttac
      421 ttcttcatcg atcgaatcga tagcatacca tgaacgcaag ccccaacgat tatcccctaa
      481 tgagataaag ctgccgtcaa tattcaagtc agtataaaat tgtgcgagtg aatcacgaat
      541 ttcactgtct gatttcccaa gatagttttg gatctggtta gctaaatcag aaaaatccat
      601 gacatcgcca cgttgttcta agatcgcgtg tgctacttcg atcattgata attcgctttt
      661 gtttgcacct tcaaatacac taatttccaa tcaggacacg tcctttcaca gtctactcct
      721 tatcatacaa aatcagacac aaaaaatcaa acttttcttt tatatttacg ctgtaaaatc
      781 caaggtcaaa taatattcgc cgattttttc cggtcccatg aacagatcat aatcgattcg
      841 aaccttccct cctgtgggac ggtctttcaa actaacatgc agttctttcg taaaagtagc
      901 aatctggatc ataccgtatg gtgttcgata ggatgtttct aaccgttctt tatagccaaa
      961 tttcaaacgc atccgcattt ctcctgcacg gatcagctgt acatgaccat ctggaactaa
     1021 tttgatcgtt acaggaatct catcgcccgt ctcctcctga atctctttat aacgtatgta
     1081 cagcgtatcg ccaattgtca ccatttgccc tttcaaatca aaataaaaat cttgcacttc
     1141 cccttgctgt tttacttttg tacgcaattt gattgaaatc ggtgttcctt gtgataattc
     1201 cattattcat tctcctttac ttctcatagc tacattgttc ctagtttctt ttgttttgtc
     1261 aaccaacttg tccattttta cagaaaacct tttttccaaa acaactcttt gattttagag
     1321 tataatattg tagaaaatat gaatttatac aaaggagccg gttgaatgaa ccctgcgatt
     1381 cgactagatc agcacattta tcaaaaaaac gacttttccc cttttgcatt aacagatata
     1441 ttaacggaat attctaaaga acaccagcaa atctttttac atttctggca atatgactgc
     1501 acagtcatct taggtatgaa ggatgttcga actccttttc taagtgacgg gatccaaaca
     1561 ctgaaagatg caggttacgc tccagtgatc agaaattcag gtggattagg gatcgtatca
     1621 gatgaaggaa ttttgaatat ttctttgatt ttcccccaaa gtgacacaaa aaaaataacc
     1681 atagatgacg cttacgagca aatgctccta ttgactaaat acgcttttcc aaaggcaccg
     1741 atacaagcat tcgaaatcac ccattcttat tgtcctggaa cttatgattt aagcatccat
     1801 ggcaaaaaat ttgccggtat cgctcagcgt cggatcaaaa atgggatttc tgttatgatg
     1861 tacttaagtg tcaacggtaa tcaggaaaaa cgcgggcaag tcatgaagcg attttatcaa
     1921 tctgctttaa aagaagactt tggtacaaat ggctatcctg cagtggatcc tgctacaatg
     1981 gcaacacttg aagaactgct tgaccaaccc ctttttgtaa aagaagtcaa agaagcattt
     2041 gcaaaagctt ttgaaaagct gtattctgca agtgatttac aggattctac cgactggcta
     2101 aaaaaagaaa tccagctgga gcaatggaac aaccaaatcc agagaatgga acaacgtaat
     2161 aacataaagg agctacatta tgacaacccc ttataaatat caaatgctgc ctatggaaaa
     2221 agtttttcgt gatccagtcc ataactatat ccatgtccag catcaagtaa tcttagattt
     2281 gatcaattcg aaagaagtcc aacgtctacg acgcattaaa caattaggca cttcttcttt
     2341 cacttttcac ggagcagaac atagccgttt tactcattca ttaggcgttt atgagatttc
     2401 aagacgaatc tgcgatatct tttctcgaaa tttttcaaaa gaaaaaatag gcaacggagg
     2461 ctgggatgat caggaacggc tggtgacttt atgtgcggca ctgttgcatg atgtcggtca
     2521 tggtccttac tctcatacat ttgaacatat cttccatacg aatcatgaag ccatcactgt
     2581 agagatcatc acttctccag aaacagaagt gtatcagatt cttaaccgag tagaagaagg
     2641 ttttcctgaa aaggtagcta gtgtgatcca aaaaacttac cccaacccac aagttgtcca
     2701 aatgatttct agtcagatcg atgcagatcg aatggactat ttgcttcggg atgcttattt
     2761 cactggaaca gaatatggaa catttgatct tacccgaata ttacgtgtga ttcgtcctta
     2821 tgaaggcggg atcgcttttt ccatgagtgg catgcatgca gtggaagatt atatcgttag
     2881 ccgatatcaa atgtatgtgc aagtgtactt ccatcctgtt tcccgtggta tggaagtaat
     2941 tttggagcat ctgcttcatc gcgcacacga gttgtatcaa gaagatgcca gcagttttga
     3001 actccattct caattacttc tgccatttct tgacggaagc tttactttag aagaatacct
     3061 caaacttgat gatggggtct tgggcactta ctttactcaa tggtctgaat ctgctgatcc
     3121 tattttaaat gatttagcta aacgtttttt gaatcgtaaa ccactgaaat ctgctacttt
     3181 ttctggcaac cgggattcaa aattagtcca agaactcact ttgttagtag aaaaggtcgg
     3241 atataatcct gtttattaca ctgctgtgaa ttctagctac gatctacctt atgattttta
     3301 tcgcccagaa caaggcagac atcgcacaca aatcgagatt ttgcgaaatg acggaacttt
     3361 gatagaatta tcacaggtta gtcagcttgt tgctgcatta gcgggacaag aacaaggaga
     3421 tgagcgattt ttctttccaa aagaaatggt tgatccgtcc ttgcgtgatc attatgattt
     3481 atttgacgaa acttatcaag aatttgccag ccatatccgt aatggcgcat tgattgaaat
     3541 aaattaaaat atattggagg aaattatgtc tatcaaacta gtcgctatcg acatcgatgg
     3601 tactttatta aatgagaaaa gagaagtaac aaaagaagta aaagaagcga ttgccgctgc
     3661 tgttgcaaaa ggtgtttcga tcgttttatg cactggccgt cccttaccag gtgtccagga
     3721 acagttaaac gagttgaacc tttttcaaga taatgattac gttattactt ataacggggc
     3781 actcgtacaa caaacgaaat caggtaaaat catcgcccgt cacggactga cacatgaaga
     3841 tttcctagag atcgaagtga tggcacgccg tgtcggcagt catctccatt cgatcgatga
     3901 caaagcgatc tacacgccta atcgagatat cagcgcttat acgatccacg aagcaagttt
     3961 agtcaagatg ccgctaaaat atcgaacacc tgaagaaatg acaccagaca tgaacatcgt
     4021 caaaatgatg atgatcgacg aaccagaaat tttagatgca gcaattgcgc gtttaccgca
     4081 aactttccgt gataaataca caacggtcaa aagtgcgccc tattacttcg aagtactaaa
     4141 taaagaagca agtaaaggtg cggcagttgc gaatctcgca cagcaccttg gtattgatca
     4201 agatgaaatc atggctatcg gagataacga gaacgactta tccatgattg aatatgctgg
     4261 tctaggagta gcaatgggaa atgccgtttc tcttgtcaaa gaagcagcaa atgtcatcac
     4321 ttcatcaaac gacgagcacg gtgtagctga agcaatcaaa aaatatgtac tgtaataaat
     4381 aaaaaaactg agagaaagct gatctactcg ctttctttca gtttttattt ttttcgatcc
     4441 atctctcgac tgcccatcgg tggctaccac gtttcaattt cacaagcgct tcttgagggc
     4501 tcacccacca aattttattg gtcttttcag ttggttctcc tatacgcttc caagaatcag
     4561 caacaaagaa atatcctgga ttataataat aagttgcacg ataattcgag taaaaatact
     4621 catcagcttc tcccagataa gaagaaattt ctgccacaat tcccatttct tctatcagct
     4681 cacggacaat cgcttcttct ttcgtctctt ctccttcaat ttccccgcca ggaagaaaaa
     4741 aagcaccatt tggagcctcg attactgcaa tctgctgatc tctttctatg acaatatatg
     4801 ctgcataacg ggtttgataa tcagcaaatt gctttttttc accaaaaatt ggtttatcca
     4861 tgaatagttt ccaccttaac agttttattt tctatccatt ttacatgaga aatttctcat
     4921 gaacaagcac aaatcaacta tttccttact tctttttgtt atgattgagt aaaaggaaaa
     4981 ggagagatga atctatgaaa atggctcaca catgtgttcg agtgaaagat cttgaagcat
     5041 caatcgaatt ttatcaaaag gcatttggtt ttgaagaaag ccgccgccgt gattttccag
     5101 aaaacaaatt tacacttgtt tatttgacac ttcctgggga tgactatgaa ttagagttga
     5161 cttacaacta tgatcatggt gcgtatgatc taggagacgg ctatggtcat atcgctatag
     5221 cagctgatga tctagaaaaa ttacacgagc aacataaagc tgccggtctt gagattactg
     5281 acttaaaagg attacctggc actgcgcctt cttactactt tgtcattgac cctgatggtt
     5341 ataaaatcga agtcatccga ggatagttag tatggggcat tttactcttg attgaataaa
     5401 aaacaaacac caataattaa aatgagacgc aaacttttca ttaaattgaa aaagtttgcg
     5461 tcttttttta acaaccattg tagctataag gttgccactc tccaccaatt tatttttaat
     5521 tcatttgaaa gaaatcaaat aaacgaggta cctttagaaa acaaaactgc ttttcctcct
     5581 attatcacac gatcttcttt taattcacat tctagatccc cgccccttga tgacatttga
     5641 taagctgaca attttgtttt attcattctt tctgcccaat aaggaataag attagaatgt
     5701 gctgatccac atactggatc ttcattgatt gttaattttg gaaagaaggt tctagaaaca
     5761 aagtcttttt gcacactttc tgctgtcacg atgactccta ttcctaattc aaaatttttg
     5821 atagctgtaa aatctggctg caatttagcc accgtttttt catcttctaa tacgaaaaat
     5881 aaatcacgtg ctaagtaggc ttctttaatc ttagccccaa tcgcttcttc atattcagac
     5941 aatatcggca cttttttcgg cataatacta ggaaaatcca tgtaataata atctttaatt
     6001 cttgtcacaa atagattacc gctttgagta gaaaatttaa tcgtttcatc aggtattttg
     6061 taatagttaa ataaaacaaa tgctgttgct aatgtcgcgt gaccacaaag atcgatttct
     6121 cgatctggcg taaaccatct aagctcaaat tcttggtttt tcttcactgt aaatgctgtt
     6181 tcagataaat tgttttcaat ggcaattttt tgcatcgtac cttctggcag ccattcttct
     6241 agcacataaa ccgctgctgg atttcctttg aacacctcat ctgcaaatgc atcaactacg
     6301 taataatcta agttcatttt aaagcccctt tctctaattt gttataaata tattctgcac
     6361 aacaaagatc ttcaatagct agaccaactg cgtcgaaaat cgtcggcctc gattctcctt
     6421 tataaaatgt ttttttctca gaaactaatt cgcctaatgt accaacaata caagattcct
     6481 ctaaatctcc ttctgccatg gctataagca ggtccccgct ttctgttaag gcagctgagt
     6541 aatcatctac atagatttga gaagtttcca tcaacgatgt acttatctct cttgtatcag
     6601 gagtaaatgt gcccacagca ttgatatgtg cgtcttccct tacccactct ttttgaagaa
     6661 atgcctcttt actagaggtc aacgtacaaa tgatttccgc tggacgtact gcatcttcta
     6721 ctgatattgc atttataaac gttattttag gatactgttt tttagcttta gcgataaaag
     6781 tatttcgtct ttcttctaaa atatcgtaaa caaacactgt agtgatggga cgaatggtag
     6841 aaatcgcagt caaatgcgac aatgcttgtt gaccagctcc aattaaagca agtgtttctg
     6901 catgcggatt tgctaagtaa tctgttgcca gcgcagatac agcagctgtt cgaatccagg
     6961 tgactgcatt tgcgtttatc aatgctttgg gtagtccgtt ttgagcatca aacaagaaaa
     7021 tctctcctaa atgagaaggt aaatgatact gatgattatc cggaaaagcg gtaatgactt
     7081 ttgtgccaaa atatctgttc tcccccaaat aagcaggcat catcgcaaac atatttttgc
     7141 cttttccgtc aggtaaaatt tccaccaatc gaggattttg gctgatttta ccactttcat
     7201 aatcaaaaaa acattttttc attacttgaa tcgcttcttc aaaactaaaa gaagctacta
     7261 cttcctcaaa attaataatt ttcatagaca attccctcct cacaaaagat taacttctaa
     7321 aatcaataca atcaatggta aaattgtatg gtatacaatt ttaaggagtg gagaaaatgc
     7381 ctattaattc ttatgacaat tacccgttaa cttggcgacc agacaaacaa aaattgaagc
     7441 gtccttatta tcgatcgttg attcaacaat tagaacaaga tattttaaag aataaattac
     7501 agaaaaacac tcagttaccc tcccaaagag agctagctga ttatttagat ttaaatttta
     7561 ctaccgttgg caaggcttat aaatatggaa ttgagaaagg tttgctgtat acgaatattg
     7621 gaagtggcac atttatttct cccaatgtct ttgagtccat caccatctct accaatgaag
     7681 taaccgaaca tatcattgat tttggtctgg ttagttcttt tgaagaatgt aataaattcg
     7741 ttgtcccgtt tattcaatca actagtaata acccaaatct tgttggtttg cttaattacc
     7801 aagatcccaa aggcacaaat tttcaattga ctactgcagc aaattggtta aaaactcaag
     7861 gagtcattcc gtccttagaa tctctatcta ttgtttccgg tgttcaaaat ggattggcta
     7921 tcacactttc tgcacttttc tctccaggtg atagaatagc aacagaccga tatacatacg
     7981 ctaatttcat tgaattggct cagttatatc atttagagat cgttccaatt gattttgacg
     8041 agcaaggaat gcgacctgat atattgattc aggaatgtag aaaaaagaag attcatggtg
     8101 tctttcttat gccttcttgt aataatccta tagggtttca aatctctcac aaaagacgag
     8161 agcaattagc cgaagtgatt gaaaaagaag gtctatgggt tattgaagat gatatccatt
     8221 catttttaac aacttactat caacatacga tcattcctcc tttccaaaat ttattaccta
     8281 accaaacaat ctacttagct ggaatgacaa aatttatttg ttctggattg cgagttgctt
     8341 atcttgtagt tccagtacat gtccaaagca agatagaaca agcaattttt aatataaatg
     8401 tgaaaacttc tggattagat gtggaagtca tcactcaaat cctccattca aatgaagcaa
     8461 ataggatctt agaaactaaa ttaagtctta caaaaagagc caatcagctt tttgattctc
     8521 ttttcaaagt agaaaaacca acgaatcgtg tcccttacta tcgaattctt ccagttgatc
     8581 ctgagattga ccctaagata attgagaatg actttcttgc taacggtatc cgactttatc
     8641 attccagacg ctttactaca cgtgcgcaac cagatgcatt cgttcgaatt tccctatcct
     8701 ctaataacct tgaaatttta gcttctggac taagtatcgt aaaagaaaaa ataaaaaaat
     8761 atgaattata acctattgat aaataaagga atcaaatctt attgattcct ttcaataaaa
     8821 aagaggcact acccgaggtt caacaacggg tagtgcctca aaaaataaac gaacattttg
     8881 cacttaaagt atccaagaag caagaaaaat tattttattt tcttatttta ctaaatcact
     8941 aggtttctgt cagaagatga aattatccaa tcatgacact ttcttctgga tatttgtatt
     9001 ttgattgtgt tctttgtgct tttgtcatca gagagaagac cacagtcaat acaccaactc
     9061 gtccaatgta cataagcgct ataatgatca atttccctat tgcagtcaag tctggcgtca
     9121 atcccatcgt aagacctact gtaccaaacg cagagaagac ctcaaaagca atatattcga
     9181 tgccagaagt tttaggtatc gtttcagtca ccgataaaat catgatggcg actacacaca
     9241 acgtcaacgt aacaaaaaat aaagtaagtg cccgcaacac tgcgccttgt cggatggtac
     9301 ggccaaatgc ttccgctctc gtacgtcctt tcaacatcga acgcatctgg atcagcaaca
     9361 cacctaacgt agttgttttc aatccgccgg ccgtcgaccc agatgttccg ccgatgtaca
     9421 tcaaaaacat cgttaaaatc agacctgcat ggctcatatg gaggtaatca actgaataat
     9481 atccagccgt tctaggtgtc acactcataa aaaaggtgtt tgccaatcgt tgaaacatat
     9541 tcccttccac tagcgttgcc ccattgcgct ctgtgaaaaa gaacacaacg aatccgccta
     9601 ataaaagggt acctgtaacg gttaatgcga tttttgaatg aatcgtcatc ttctttttct
     9661 gatgatattc cagtaaatca cgccatacga tgaagcctaa cccaccagaa atgatcaaag
     9721 cagataccac catgatcaaa taaacatttt gctggtgatt ggctaaactg tctcctaaca
     9781 aatcaaaccc tgcattacaa aaactagaga ttgcatggaa caaactgtac cagatacctt
     9841 tcaaccagcc aaattcaggc acaaatacaa aagataaagc aaccgtacca atcaattgga
     9901 taacaaaggc caattttagg atgtaaagca tgagcttcat cacacctgat acttcttcta
     9961 gattcagcgc ttcttttaat ataatacgtg tgctgagact gactttcttc tttatcaatg
    10021 caaagcatag aatcggaacc atcataaaac caagaccgcc gatctcaatc aaaagcatga
    10081 tcaaaaattg accagcactg ttccaatgct ctgctgtatt caatgttgtc aatcccgtta
    10141 cacagacagc agaagttgca gtgaaaagcg catcgataaa atttgtccca tgcccactat
    10201 tactaaaaaa tggtagggta agtaggccgc cgccaactaa aatcaggaga aaaaatccta
    10261 atgcgagaag ctgaacagcc ggcaaacgtt tctttatttg tttgctttgt ttaatcatcg
    10321 ttttcctctt ttaaaaaaag ataccactta gtcagtggta cccatatttt ttactttgat
    10381 caaccttgtt acttctgcgc gctcgttttc ttccagcttc atacgaatat aatagatcgt
    10441 ttcttctaat tgagggatcg tcatatattc taatgcattg actcgccgac gtgtcttttc
    10501 gatttcactc gccatcagtt gacaagtttt ttcgatttct gtcagttcca aaagttttgg
    10561 aagcagggta gtaaaccgat cgattgaccg atccagttcg gcactggaat tcaaataccc
    10621 gtattccaaa ggtgtgtctg tcagctgttc atcatattca aaattcatca caggtacttt
    10681 tacactcatg atatttttgt ctacgataga caaatcaacg ttttccgccg gcaaaacaaa
    10741 taattcttcg atgaaaggtt ccgtgatcga agcatttgcc aatacaaaat cagacattgc
    10801 ttctgtcata gactcttcca tttcttgacg cagctggttg ttctttcgaa tcaacaggat
    10861 gaattgacgc atcagttcat cttgcttatc ttttaataat ttatgccctc ttgtggcagt
    10921 tgtcaactgt tttttcaatc gagtcaattc catgcgggtc ggattcacat tcaatcgtgc
    10981 catcagaact cacttccctt ctggcagata tttgtccagc atgtcgtctt tgattcgttt
    11041 taattcggtt cttggcaaca tcgatagcag ttcccaccct agatcaagtg tttcagtaat
    11101 cgaacggttc gtgtagaatc cttgattgac atattctttt tcaaaccgct cagcaaactt
    11161 cgcatagatc ttgtctacat cagataaagc agattcacct aaaactacgg ctaattcttt
    11221 tgcctgtttt ccttgtgcat aggctgcaaa aagctgattc atcgtagctg catggtcttc
    11281 tcttgtcttt ccgggtccag ttcccttatc ttttaaccgc gaaagagacg ggagtacatc
    11341 aattggtggc tgaatcccac tcttgtaaag ctctctagtc aagatgatct gtccttctgt
    11401 aatataacca gtcaagtcag ggattggatg agttttatcg tcttctggca ttgttagaat
    11461 cggaatttga gtaaccgatc cttttagtcc gcggattctt cctgctcgtt cgaataaagt
    11521 agctaagttc gtgtaaagat acccaggata tcctcttctt cctggaactt ctcgccgtgc
    11581 tgcagaaatc tctcgcaatg cctctgcata gttggtcatg tctgtcatga taaccaaaac
    11641 atgcattccc ttttcataag caagatattc tgcagcagtc aaagccatac gaggagtagc
    11701 gatacgttcg attgcagggt cgtttgctaa attcataaac atgacagaac ggtcgattgc
    11761 gcccgtttga cgaaaatctt ccatgaaaaa ttcagcttct tcaaaagtga ttccaatagc
    11821 agcaaagact acggcgaaat catctgatga atcgagtacc gttgcttgtc gcgcgatctg
    11881 agcagctaat tctttatgag gaagaccaga gccagaaaat actggtaatt tttgcccacg
    11941 aactaatgta ttcaaatgat cgattgcaga gatacctgtt tggataaact catctggata
    12001 atcccgagct actggattga ttacttctcc gttaatatcc aaatgctttt caggcaggat
    12061 ctctggtccg ttatctttcg gtcgtcccaa accatcgaat actcgaccga tcatgtcttc
    12121 cgatacacct agttctaaag gatggcctaa aaatcgtaca gaagagtcac gtaaattgat
    12181 accacttgtt ccttcgaata tttggaccat tgctttatct tcttggactt ccaatacttg
    12241 tccacgacga atctcgccat tttgcatacg gacttcgatt aattcttcat atttgacacc
    12301 agcaactttt tcaaccgcca tcaatggacc gacgacttca ccgatcgtac gatattcttt
    12361 aatcattgct catgcctcct tctgaaacga tcgtttgcat cgtttgcttg atttcaccaa
    12421 taatttgatc tagtttttct aattctgttt ccgggatata tttgcttcgt ccgatccttt
    12481 cacgaatcga tacggtaccg tccatgatct cacttaaata agcacctaac gacaacgcac
    12541 ggcgtccttc ttcaccaaag gtcaagatca tcttcaacat tttgaattgt ttctctcttg
    12601 aagtgaatgt atcgacatca tcaaaagcat tttgctgcaa ataatcttca cgaatggatt
    12661 ttgccacttc taatgtcaga cggtcattat ctgaaaggga atcaatccct accaaacgca
    12721 cgatttcatt cagctgctgt tcttcctgca aaatacgcat gccctcggtg accatggatg
    12781 cccagtcagc ttgaaggata tgatccatat acttccctac ttctgttgaa taaagagaat
    12841 agctttgtat ccaatcgatc gaaggaaaat gtcttttttg ggctaaactg gaatcaagac
    12901 cccagaatac ttttacgacc cgcaatgtat tttgtgtgac tggttccgaa atatcccctc
    12961 cagacgggga tacagcactg attgcggtta tacttccttc gcgaccttct gatcctaatg
    13021 caatgactct tccagatcgt tcatagtatt ccgcaagccg tgaaccaaga taagctggat
    13081 aaccttcatc accaggcatt tcttccaaac gtccgctcat ctcacgaagt gcttctgccc
    13141 atcgtgaagt ggagtctgcc atgatagcaa cgtcataacc catgtcacgg aaatattccg
    13201 caattgtgat tcccgtatag atagaagctt ctcgagctgc tactggcatg tttgatgtat
    13261 tagcaatcaa taccgtacgt tccatcaacg attctcctgt attagggtca atcaattcag
    13321 ggaattcatt taaaacatcc gtcatctcat ttccacgctc gccacatccc acatagacaa
    13381 ccatgtccac atcggcccat tttgcaattt gatgctgaac gactgttttc cctgcaccaa
    13441 aaggaccagg tacagctgct gctcctcctt ttgtcactgg gaagaatgta tcaatcactc
    13501 gctgtccagt gatcatgggt gcatccgggt ttaatttttc tttgattggc cgactacgtc
    13561 ttactggcca tttttgaagc atcgtcaaat ttttgtcacc attttcggtt tcaatgacac
    13621 atactgtttc atcaatagtg aatgaacctt tttcgattgt tttgatcgtt ccttttatcc
    13681 catttggcac catgatttta tgttttataa cttttgtttc ttctacgatc ccgataacat
    13741 ctccagcact caccgtttct ccggcttcta ctgttggttc gaaccaccat tttttctcat
    13801 ggtccaatgc gggcaattga actccgcgcc ctaagaaatt actttgtgtg atttccataa
    13861 aggtatctaa tggccgttgg attccgtcaa acatttgtga aatgatccct ggtcctaatt
    13921 caacagataa agcttcacca gtagagcgaa ccggttcgcc tggaccaatt ccagaagtct
    13981 cttcatatac ttgaatagac gccacatcgc cacgcatctc gataatttct ccgataactc
    14041 ctagatctcc cactagacac atgtcttgga tactggcatc agacatgttc tcagccatta
    14101 caagaggtcc ggagactttt atgatttttc caatttgcaa aatttctatc tccttttcat
    14161 actataagat attttgacct actgcttttt ctacgttttt ttgaatttct tccagcccta
    14221 tccctaatga tccttgatga ttgggaatca atacgatagc aggtatcatt tgttccttgt
    14281 aacgttcgat cgtttctggg ctcaatgctg cacattgctc tgtgacataa atcacgccat
    14341 actcattctt tgccatttct tcaattgcct gacgaacggt ttgcttggtt gtaccataac
    14401 gaacatcaaa gccaaacaat ttgaatggca aaacagaatc tttgtcgcca actacaccga
    14461 tcttatgcgt catagacttg tctcacccgc tttctgattg tttctggttc gaaatggctt
    14521 cttttgccga taaccagtaa acgaaggttt ttgctttcta cctcttttgc gttcaaaaag
    14581 gctagtagtg gcagtggtcc gaaagcctgg gtttgtgctt cttgatacaa tgaacttaga
    14641 taatcatctt tcaactgttc aagctttacg agatcaagat ttccctcacg gatgacaggg
    14701 cgcagaaaat cagcataatc tgtctctaat agaaaattcg tgtaatcttc catatcttta
    14761 tctacaaagt tcaaaaatac ttctttttcg atacttcccg agctggaaag taccgtcgtc
    14821 atgaacccat atgttctttt ctgcaatatt cctcgagcca tcgttgtgat attggtcaag
    14881 tcaataaaag caatgacttc tgtcagcaat tcttgatatc ctagcttatc ggcaagccgt
    14941 cgctgctctg tcaaaaactg tcgatcataa atcacgtcaa ttccctgaag aatggtagac
    15001 tcttcaaaat aatcacgtac ttctcgtata ctgtccataa tgctttcagg gagttcattt
    15061 gaaacttgcg tatgaatggc atccttcaag gtttctaatg aataaaatcc atcgtcgata
    15121 tacaaatggt ccagattttg atttgtcatc tcggcttttg tcaaaacctt cagattatgg
    15181 aaagtatagc gcatcgtata gatccaaacg atttcaggct ccggtgctga ttcctttaac
    15241 cagcgaaata gacggctctg ctcctttaaa agattctgtt cgaagtcata ttcatatccg
    15301 tcataaatat atggactgta aatcgtggat ttcaatattt caccaagtgc ttcgacagaa
    15361 tttgtttgaa tcatccgttc aaatgtctca ttggacaaaa gttcaagctc tcttccgcga
    15421 atcaagggat ttagttcgtg gtattccata cactgatccc tccttattcg aataattgat
    15481 tggcaatctc aaaactcatc gttccttgga tatcttgtac aaggctgctg aaaagaaaat
    15541 tatactgaac tccttgatcg tttatcagaa aacccgcttg atcagcaaca gtttcatcac
    15601 ttaaaaccat cataaaggga agttcattgt tccattcatc cagcaatgtc tgagaaagat
    15661 aagctgctga tttttctcca gcaatgaatg ttactttgcc tgtcaaaggt aatgaataca
    15721 atgaattttt gataaactgg atttgttcag attcatccca attctccatt tcagctattg
    15781 catctgcaaa caatcgtcgt aaaaattcct gcttcgcatt caaattttct tgtcgtactt
    15841 ctacttgttg gcgattccta agctgacggt attttttttc aagtaattcg atttgttttt
    15901 ctttttgttt ttgatgttct gtttcgattt gcgtacgttt ttgttcaaag ttttgatcga
    15961 tcttcattcg ctcttcttgc tctaacgccg ctctttcttg ctctgctgct tcattcatct
    16021 gagagatgat tttttcaatg gcgttcatct gcttttcctc ctttatgctt aggcattcaa
    16081 tactaatagg aaggaaataa cgaaacctaa aatcgcatat gtttcaacca tcgcagcaaa
    16141 aatgattcct tttgtagcat gttcaggttt tttcgctaaa atttggatac ctgctgctgc
    16201 aactttccct tgggcgatac ctgaaaacaa acctgtaaaa gcaatcggca gagaagcgcc
    16261 tagaaagttc aatccttgta caactgacat atcatttcct aagttgataa aaatcaaaaa
    16321 ggcgataacg aatccataca acccttgcgt acctggaagc aattgcagaa tcaaagcctg
    16381 tccgaatttt tcgggttgac ttgttgttaa cgctgctgct gcctcacctg tcattccaac
    16441 acctttagct gaaccaatcc ctgaaaaaat cgtcgctgtt gccatcgcta atactgcaaa
    16501 taccattcca ccattttgac taattaagta atccatcatt gtataaaaat cctccaatta
    16561 tttattgttt ttttcttatg attgatgttg acgtattttt ccgccgtttt cagcgggtca
    16621 aaggcacgtc cgcctcctgt ataaaatttt ccaaaaaatt cgacatactg caatcgtgca
    16681 ccatggacat aggcacttaa cagtgtcaaa aacatattca gtgcgtgtaa agcgatgatc
    16741 aaaaggatcc ctacactgaa tcgagcggct ggcggcatga aagcaactag catattgaat
    16801 gccgcgccaa tacttccgcc tgaaatacct agtgccatca aacgtgtata actgactaaa
    16861 tcaccgatat aaccagtcaa tccgtaaaga ttatacgctc ctttggcagc acccttgact
    16921 ttagacggcg attggataat cggcacgatt aagatacata aagcagcaat gatcgatagc
    16981 cccgcgccaa gataaacaaa tgctgtattt tttatcaaca tgacaccaat caatgcaata
    17041 acgatcccaa tcaggatccc ttgccaggca aaaccatcac tgacagcact caagtaatct
    17101 ttcctcctga tattttcctt tgctgccacg aataatccaa ccaagatctg tatcagaccg
    17161 aaaatcacgg ataaaatcaa aatcgtattg acgtcatccg tcgtagatag gattgggaaa
    17221 ggcaagcgga taccaaatag ttcttttggt aatgcctgtc cgaaaaatga actgtagatc
    17281 aatccccaga tgatcgatgg taccgagaga atctcaaaga attttacgaa acgtttcatc
    17341 ccgcggggta aaacaaatag tttttgagct agtattgtcc caatgagcat cagcaatccg
    17401 taaccaacat ctgcgaccat catcccgaag aagacaagat aaaatggtgt catccaaggc
    17461 gttggatcta cttcttcata ttttggcaga ctgtacattt cagtcagcaa ttcaaaaggt
    17521 tcgataagcg gatggttttt caatttagta gggacttcct gctgaatctc ttctgagttt
    17581 ggtgtttcga aactaacgta tatatcttct tctgatacag tttcgtttaa cacatgcatc
    17641 aaattctgtt tttcttctgc atctacccaa ccttgcaaaa cgatcagata agtggcatgg
    17701 ataaaccgtt cttttacttt ttcccgttct gcaacggcaa gtgtgacttc ttctacccat
    17761 tcaaattctt ggatatagcc gctgcactga cctaacgctg tcgttatctt tttttgctgc
    17821 tcgtgaacaa tggataattg ctgtttggtt tcttgcaatt tttctttagg attttgttca
    17881 taaggatacg tttctttatt gaacccgtat ttcccagcaa tttcttctat agcagcaact
    17941 gattttttta aagcaacata cgaaagataa acttgttttg gcgtataatc tatttcttcc
    18001 atataaacgt catcaagctt acttagttct tccttgaaac tttcaaaatt tgctgcactc
    18061 acagtcccca tctcaaaaat ggtcgtctta cttgcataca tacttggagc aatatccagc
    18121 tgttgccaat gaagaagcca ttcttcctct tcttctaaac gatcacgttt ttcagttagc
    18181 tgttcccact tttcttttaa atcaaggact tcttctaatt tattcatgaa attttcttcg
    18241 gaataatttg cttccaagtc ttgtaaggaa agttcttgac gtttcaaatg gagtttcttt
    18301 tgacggctat cgccgtggtg ttgtatgaat tggatagctt cttggatgtc tttcagccga
    18361 ttgcctaaag cgcttaattt tgcttcttta tcaataatcg tggtatctgg aaaatatttt
    18421 tctacccact ggttgtttgt tctattttga tacagatccc ggatttcaac attttggatt
    18481 ccttgtactg cttgtaaaat cctctcctga tttcttttat ctgaaatgag ggtcactttt
    18541 tccatcttag tgacggccat aagtttcttt caccctttca atgattgcac cgattgcttg
    18601 actctgatgc ttttgatatt ggtctttcaa agttgcttgc accttgtctg cttctgcttt
    18661 caatttcatt ttttcttttt ctaattcttg cttttcgaat tgttcttttt cttctaccaa
    18721 ctgtttcagc tgttgccgac tgttcgtttg gattatctcc aattcttgct ctttttcttc
    18781 tgcataggtc ttcaactgct gccgtagggt tcgagcacgc ttctcatttt cttcttctgc
    18841 ttttcggatt tgttccaaag cttctaacac ttgcttcact tcctttcgat ttaaaaaaga
    18901 ggtacatgct ttcttttttc attgaaagta tgtacctcta aagataactt agtataggca
    18961 ggccccttca ataaaatgtc cttctcaccc cttgaagaac tagatccttg aagcttacgc
    19021 ccaacctttt tatttctgct tactttttat tataacaaat taagcaatat tttcaactat
    19081 cgtcatgaat ttcacacgaa aattctcttt acagatagta acacagcaag aacagataag
    19141 tataggggca agtcttacat agtttaaatt tttttaaatc cttgttacaa ttagtctact
    19201 ttagggggaa taaaaatgaa accaatcatc ggcattgccg ggaatcaact gattcgagcg
    19261 acagatactt tccaaggaaa tcaagtaagc tatacaccgc agggatttgt ggatgcagtt
    19321 ttagatgctc aaggattacc tcttattctg ccagtcatgt ctccagattc agcgcctcaa
    19381 ctaattgaga aaatcgacaa actcattctt gcaggcggtc aggatgttag tccgcaactt
    19441 tacatggaag accctcatcc aaaactgacg gaaacaaata ttcaacgaga ccaattcgaa
    19501 caggcattga tcttggaagc attgaaacaa agaaaaccga tttttgcagt ctgccgagga
    19561 cttcaactat tgaatgtcgt gttggaggga acactttatc aagatctttc attatatcca
    19621 aaatggtccg taaaacacga acaacagccc actgccccgc agtttgcaac tcacgaagtc
    19681 aaaatcgttt cggacagttt attaagtgac cttctccctg attcatactt tgtgaattcc
    19741 tatcatcatc aggcagtcaa agatctttct cctttgttaa aagccatcgc cttttccaac
    19801 gatggactca ttgaagcagt ccaaagtaaa gataatatgc acaaaatcct tggtgtccaa
    19861 tggcatccgg aactttctca tcgtgtagat tcatctgaac agcgtttatt cgatttcttt
    19921 gtgaatgaat tttaatcgtt tttctgatta ggtcgtgagc ctgccgttca actctgagtc
    19981 acaaggagca atttcaaaaa acagcttctc atgtttgttg gatattgtga cttgtgaccg
    20041 aggagttggc aggagaacac cgtttaagga agaggtcgtg aaatcagcgc cctgacctga
    20101 gtattagaga aataaacaga aaaatagctt ctaatatttt accgtttttt gagctaattc
    20161 cgaaggagac tgcgacacgc cttttgttgc agtcgctttt tagtctattg agcaatgttg
    20221 gctagcattt ttctcaatcg tcctttcgat aatccaacag cctttggagt tgagtaatct
    20281 gttgctgtaa ttccattcct tttgtcgtat tccgattaag tttgcgtggg agctgctgct
    20341 cgatcacttt ttttacattc gtttgatcag atttttttgc aaattgttgt tcaataggca
    20401 agaacggata atgtgtcacg atttggaaac cgtaagaatt gcatagtaat gagtagccag
    20461 caatcccggt tgttttttga taagctttgg atagacctcc gtcaataaca aatgcttttc
    20521 cgtttgcttt gatcggtgac tctccttttt ttacttttac tggcgtgtgt ccattgatga
    20581 ttgctgaacc ttcttctttt gcatcgaatt ctgccaaaat tttttcactg aaccactctt
    20641 catctcgcaa gtgataatag ggattgcttt tttcgacgtg cgtatgaggc tctgtgagaa
    20701 aatagcgctc aaaagttgcc attttactct tgccgaataa aggcgaaaat ggcccgctcc
    20761 acgcatacca aatcaaatcg gtcgccaaat cttcatttgc agtcaaatct tttgcgcttt
    20821 cagttatctg tttttcaaaa aatcccagca gttcttttcc gcgataagat tggtctaatt
    20881 caaatgctaa gagctgcccc ttttcatcta caggtataca gccatggaac agtacatgct
    20941 gattgttcgt gagatacatt cccccttttt cgatcaaaaa cgtcatgtgt ttttgcattc
    21001 ttaacgagtg ctggaatgaa tacatcaaac tatcgatcac tttttgttcc tcttccaaga
    21061 attttttagg atcatctggc tggatcgttt gaaaacaagt ccctttcaac tcatagagct
    21121 gaccttctaa aacgatcgtt gattcctcat agttgatttt ttccaacaat ttacgatcgt
    21181 ccattttaaa ttcaggtcgt cgattcaaca actgatgttc tgatttgaat tggaggatag
    21241 aaagtgcttg atggatcttt tctaattctt cttgctctct ttcagataga tctttcctgt
    21301 ttttaggggt aaatactgga ttcttctgat aggtttgatg ggcaaaagaa aataatggac
    21361 gtaaatttaa tccatattct ttctctaatt caaataaata gttgtagcgt gcggcaatcc
    21421 gtaaaagcgt caataaacat gcctcagagc cgcaatacgc accaatccac aaaatatcat
    21481 gattccccca ctggatatct acagaggaaa aatccatgat tcgatccatt actttggctg
    21541 cttgagttcc tcgatcaaaa atatcaccca cgatatgcaa atgatcgatg acagattttt
    21601 ggatcgtctg cgcaagtttt gttaaaaatt tttcttcttc cttcatcaag acaagttgtt
    21661 caatgatttt ttttacatag tcttttttcc cgttgatacg atcatcgata tacaagagtt
    21721 cctcaatgat atagctatat tctttcggca atccttttcg aacttttgat cgagtgtatt
    21781 tgactgtcgt aaaccgtaaa agatcgatca aacgatggat cagctgggat ttatcttgtt
    21841 ttttgtacca atcagtcgtc aacacatatt ctggataagc cgtaaaaata gtgaattggc
    21901 tgatctccgt ttccgtcatt tgttcagaga aaaggtcttt gatcttttct ttaagatttc
    21961 ctgaaccgat acgcagaata tgatcaaaag caggaaattc gccatgtata tcgctcaaaa
    22021 ataactctgt tccttttgga agctgcaaaa ttgcttcgag attgatgatt tccgtgatga
    22081 catcttccgt tgtagggaac ttctctgaaa gtaaatctaa atagcggtcc attaaaacgc
    22141 ctccaaaaat tggtatactc aacattataa agttgttatg ctctattttc aatgaatata
    22201 tatatccaat ataataaaaa aatagaaata ggtctataca aaaaagaagc tgggaacaat
    22261 aaatgtccag cttccttcta tatttttcta tattttatgc tttttttcgt tcatttctca
    22321 taattgaaat cgattgtttc aatgaactcc ataatccgcc ttcgctatac acgagtacat
    22381 tcgatttgta catgtttgca gacaagatca tcaataagaa tgttacggct acaagtacca
    22441 acaaggaaat caatgctcca cccataccga ctgtctcatt tgctaagcga accggcataa
    22501 tatatgaaga taagaatgga atatacgaag tgatccgaat aacgatattg tttggatctg
    22561 aagctcctag tatcaatcca agcatatatc cgcctagaga tagataagtc actggcaaaa
    22621 ttgcttttgc tgtatcttct gctttgttga ccaaagagcc acataaagct gctaaaacag
    22681 aaaaaatcaa aatgccaaag atcatgaata gaagagaata ccacaagaat ggtccaatca
    22741 agttatccaa tgagataccg tctaatatat ttttgaccat ttccatatct ttgaattgac
    22801 tgtatcctat accaaaaata ataccgtata atgccatttg cgttaatgca acaagtaaaa
    22861 cacctgttag ttttccgtaa aaatgctttt gtgcagtcgt actggacagg atcacttcca
    22921 tgatccgcgt gcctttttca gaagcgatct cttgtgcaat gatctgcgca tacgtcaaaa
    22981 taataatgaa caagacaatc gttgctacat aacttacgac atattggact gtcgagttat
    23041 cttctccaac tgtcattttt ccttttgaat caaaattgac tttttgtcga gtcaaacttg
    23101 cttgttggct caagctagct acttcttctg gtgaaatgcc taattgactt gctcgaagca
    23161 tcgattgcaa gccagtcagt tgttgttgta tcagtagttg tgtactttgt cctaatgaat
    23221 tttcgctata aagcatccca tttatcttat cattctcagt tgagactact aaatacgcat
    23281 cgattttttc ttccgaaagc tctttttgtg ctttttcttc agaatcaact gctttgaact
    23341 caaaatcatc tgttttgact tggctcattt gctgactgat ttgctcatta tcagccaata
    23401 cgccgatttt attgacatca ctattttgtt gagcaaagtt tcctgctaca taaatgatac
    23461 ccagcatgat aaaagggact aatatcatga tcaagaaaga aatcgactta acatttttta
    23521 aatagacatc tttggtaata atccaaaact tacgcatcga tttcacctgc tttcaatttg
    23581 aaaatctctt ccaaagttgg cggttgctgg ttaaacatag gaatgtagcc gaactgagtc
    23641 gcacgagtaa agatttcttt cccagcttca ggatcttcta aggtaatctc taacgtattg
    23701 tcttcatgaa ggatcacttt tttcacgcct tcgatttctt ctacttcctg ttggcttaaa
    23761 ccagattcca agaataactt cgtacggcca aatgattcac gaatctcatg tacttttcca
    23821 ttcaaaacca tttttccgtt tcgcaacatg atcaaatgat cgcagatttt ttctacatta
    23881 tccatattgt ggctagaaaa aatgacacat gaaccatgtt ttttcaattc caatattcca
    23941 tcttttagca attcagcatt taccggatct aacccgctga atggctcatc caaaatgatc
    24001 aatttaggtt catggattaa cgtggcgatc agctggactt tttgctgatt tcctttagac
    24061 aaagatttta ctttatccgt ctttttccct tttacctgaa atttttccat ccaaaaatcg
    24121 atttttggtt caatctcttt tcttgatttc cctctaagtt ctgcaaaata aagcagttgg
    24181 tcttgaatcg ttactttcgg ataaagtccc cgttcctccg gaagataacc gatgacatca
    24241 taatctttct ccccaagatg atggccattc cataacactt ctccttggtc ttgtgtcaaa
    24301 aaatccaaga tcaaacgaaa agtcgttgtc ttcccagccc cgttttggcc aattagcccc
    24361 atgatttttc catcggggat ttggaaagag acatgatcga cagctgtgta gttcccaaaa
    24421 gttttaacaa gatccttgac ttctaacatt tcttcactcc ttcttcaaga aatcacatat
    24481 atccttagac accagacgta agcgatttat tatttaccaa caaaacgtaa acgctctttt
    24541 tgttgtacat aaaatgatag gacactttac tttctttgta aagtgccctt tcctcattcc
    24601 tgatgtaatt tatcaaatac gttttgtgcg acatttttcg gcagtccgat ttcttgtaat
    24661 tcttctgtcg tggctgctgt gatatttttc aacgatttga actctttaag cagcattttt
    24721 tttcgttttg gtcctaaccc ttcaatttca tctaatcggg aagcaaaact gtttttactg
    24781 cgtagctgcc gatggaaagt gattgcgaat cgatggactt catcttggat tcgctggagt
    24841 aagaaaaact ctggtgaatt tcgctcaagc ggtactacag ccaaatctat accgaatagc
    24901 aactcatttg ttttatgttt atcgtttttt gccatgccag caataggaat atcgattcct
    24961 aattgatttt ctaagacatc ttttgccaca tctacttgtc cttttcctcc atcgatcaag
    25021 atcaaatcag gtaaaggcaa cccttcgcga atcactcgtg aatatctgcg atagatgact
    25081 tctctcattg atgcataatc atccggtccg ttgactgttt ttattttata tttccgatat
    25141 tcttttcttg ccggttttcc atcaatgaat acgaccatcg cagccacagg attcgtcccc
    25201 atgatattag agttatcaaa agactcgata cgtattgggg tcgggatatt catcgcgttt
    25261 cctaaacgtt caactgctcc gatcgtccgt tcctgttttc tggcaatcag atcaaaccgt
    25321 tcattcaaag caactgaggc attcttattc gccaattcaa ctaatttttt cttttctcct
    25381 cgttttggtt gcagcacttt cgtagctaac aaagcttcta cactgggctt gtcgatcgta
    25441 tcaggaatca agacttcttt aggaatgaaa tgctcatttt cctgataaaa ctgaccgagg
    25501 taagtcaaga aatcttcttc tgcttcgtta tagaaaggaa acattgaaac atctcgttca
    25561 attagctttc cttgtcttac gaaaaacact tggacacaca tccatccttt gtcaatcgca
    25621 taaccaaata catctcgatc cagtaaatca gtatttgtca ttttttggcg agtcatgatt
    25681 gtctcaatgg cacggatctg gtctcggtat tctgcagctt tttcaaactc catgttttca
    25741 gcagcttttt ccattttctg atggatctct gactcgattt tttcgtgtcc tccgtttaaa
    25801 aactttttca cttcatcaac gattttcgta tagactttcg gatctacgtc gaaataataa
    25861 ggacacagac attgtcccaa atgatagtaa agacaaggct ccttcgtctg actaggtttg
    25921 cattttctca gaggaaataa tcgatcgagg atcttctttg tctcattcgc cgcaccgaca
    25981 tcgggatatg gtccaaaata cagtgctttg tcttttagta ctttacgagt aatcaccaaa
    26041 cggggatatt tttcattcgt tatcttaagg aaaggatagg ttttatcatc ctttaacata
    26101 atgttgtatt taggatcatt tttctttatc agattgatct ctaagagcaa tgcttcgata
    26161 ttcgattctg taacgatgta ttcaaagtct tcgatttcac taacaagccg ttccgttttc
    26221 gtattatggc tgccagtaaa atatgagcgg acacgatttt ttaatatttt ggctttgccg
    26281 acataaatga tcgttccatt tttatctttc atcaagtaac agccgggctg atcaggaagt
    26341 aaagctaatt tgtttttgat tcgttcgttc atctggtact ccccttcata ttcaattaaa
    26401 gttattatag catgtctgtc aaaaaaacaa tccagcctga caacgtaatt atactcaaaa
    26461 aatattaatt tgaataacct taactcaacc gcaaaaaaac cgaggacgga actggattca
    26521 gtcccaccct cagcttattt ttgctgtaag tttttataaa tattgatcga tcatttgacg
    26581 tagttgttct tttgaatgaa caccaacagc tttttctact acttgaccat cttttttcaa
    26641 cattaatgtt ggaatgctca tgataccgaa ttgttgagga gtttctggat tttcatccac
    26701 atccatttta gcaattctga attcttcttc atcatattct tgttccaact gatccaaaat
    26761 cggtgcttgc atacgacatg gaccacacca agttgcccaa aaatcaatca atacaagtcc
    26821 tttatctgtt tcttcattaa atgtcttatc tgtaattact tgtgacatat caaagacctc
    26881 catttttaaa atctatgatt agtatagcat ttctatactt ggatagcgaa ccatctgctt
    26941 acctttagta agctttactt aaatttaaca attgtcgcac catttccgcc ttggtttgcc
    27001 ggtgcaaact caaagctttt gacgctccga tggtttttaa gataatcagt aattcccttg
    27061 cgaagtgcgc ctgttccttt tccgtggaca atagtcactt gaggataacc agctagaatg
    27121 gcagaatcca aatattgatc tacctcactt agcgcttctt cgtatcgttt tcctcgaagg
    27181 tccaattgat tcggcacgtg actactagat tcagatcgta cagcatggat aactcgttgt
    27241 gtcggctctt tttgaggagc aacaggcgtc agctcgtctt cggacacatt catttttaat
    27301 ataccgattt ctacttgcca ctgattcccg tttttacgta aaagcgtccc tctttggccg
    27361 taagtcgtaa ccaacacttc gtctcccggt ttcaacgttt tttgttcttt cgccttcttc
    27421 aatactttat ttttcttcaa agtttcttct tgatgtagat ttgctaattg ggtctttgca
    27481 tcgatcaact gatgctcttt cacatttcct tgcccaatct gctgttgcat tttacgaata
    27541 tcagcaatga ttttttctgc tttttcttcc gcctctgaca cgacttcgtt tgcttttttc
    27601 tttgcttttt ccatttcttt ttcacgttct tcaaagaaat agctgtaagc ttctttcaaa
    27661 tcatcgtgaa gttcttgtgc ctcagaaaca aaatgacgca tttccagata ttcggtctct
    27721 gccattttcc ggcgattctc taaatctgtg atcatttcgt ttaaatcttg gctctcatca
    27781 ttcatcaact gcttcgcttc atcaataact tctgtatcaa gacctaatcg gctagagatt
    27841 tcaaacgcat tgcttcgtcc aggaacgcca atcagcaaac gatacgttgg acttagtgta
    27901 tccacatcga actccatgct ggcattaatc gtatttgccc gattgtatcc ataaactttc
    27961 aattcaggat aatgcgttgt tgccatcaca taagcagact ttttaccaag atcatctaag
    28021 attgcgattg ctagagcagc accttcttgc ggatctgttc ctgcaccgag ctcgtcaaat
    28081 aataccagac ttttttcatt gacttttgat aaaatatcta cagtgttcgt catatgagaa
    28141 gaaaaggtcg atagactttg ctcaatcgac tgttcgtctc ctatatccgc aaatacttcc
    28201 tcaaaaatac cgatttgact ttcttcacct gcaggaatcg gtaatcctgc ttgccccatc
    28261 agttgtaaca gacctaaagt cttcaacgtg atcgttttcc cacctgtgtt aggtcctgta
    28321 atgacgatgg cttgatagtc ttttccgatt gtgatgtcat tagaaactac tttttcctga
    28381 tcgatcagtg gatgtcttgc ttgtttcaag acgacatgat tgtcttcact gattcccggc
    28441 acgatggctt ttacttcttt accaaatcgt gcttttgcat tcatcagatc cattttacca
    28501 atcacataag cattgtggat gatttcttga cgatggggaa caagttcagc agaaagttca
    28561 cttaaaatac gttgaatctc atttcgttca gcgatttggt attgacgcaa acgattattc
    28621 aaatccacca cttgttttgg ttcaataaat aaagtctgtc ctgaagcact ttgatcgtgg
    28681 actacaccgc caaatacacc gcggtattct tgtttgactg ggataacgta gcggtcattt
    28741 cgcatggtga tgatcgcatc acttaaatac tttgcatttt tcccacggac gatcccatct
    28801 aactgttcac gaacagcttg ttcacttcgc cgaatgttct gtcttatgct ctttaactca
    28861 ggagaagcgt catcagtcac gcgtccgtct tcatcgattg cttcctttaa acggcggctc
    28921 aaaacaggga tcgtgactaa ctgatctgcc cacatataca aacgtgcaaa ttcgatttca
    28981 ctgtcagaaa gatcatcaat gaaacgattc aattcgcttg tcgtagacaa tactctggaa
    29041 acttgtgcaa gctccactcc attcaagtct gctccgatct cgatccgttt catatgggga
    29101 cggatatttt caagtttagg tatagggatc ccgcctcgca gacgttgcac tttcaatcca
    29161 tcctcagttt cttgaagcca gtttgtgatc gtttgtttct cactcactgg taccaattct
    29221 gccagttctt cttttccttg tgcagtcact acaaattgtc tgaccatctg tttgactttt
    29281 tcaaattcca atgtttcaag gattcggtta ttcattttat caccttatct ttcactatga
    29341 aaaaagagct gtgccggaca tacgctacgc agccttctta aataaactac tctgtacaaa
    29401 aaacgaaata ttttccagca ctattttcct taattcaaaa tattcgttac ccaaagctca
    29461 tacatcttgt tggtaagaaa tggcgttctc tcaacgataa aacgagcgaa actatgctcg
    29521 ccgaattgat tttggaccac actaatggaa caaaggcaag taactgcaaa atcaaaaata
    29581 tagtaacgta agccatgatt agtgacagga tgcctcctgc aatccagtct acctgtctca
    29641 agatcggcac atacgtcaat ccatggacaa aaacccccac aaatcgggta agcagccagc
    29701 caattagcaa aataatcaga aaagcagttc ctgcataaaa agcttcatct aggtgaaaag
    29761 caaatacctg atcaaaaaat gctaactttg atgttggggt gacagctggg tatggaatgt
    29821 ataattccaa atgacttgct aatggtttgt aaaaatgctg ggcagcaata aatgacaata
    29881 catatccaat cgcgaatatg atttgcaaag caaacccgcg acgtgctcca gaaaaaaacg
    29941 caattagcaa taaaaataaa atcagcaaac tcagcatgct acgttccttt ctcttctaac
    30001 ttttttaaat ttttcttatt ttgataaaag gatcttaatt agaagatttt tgtttaattt
    30061 gtccttttcg tttctcgttt aaaatctgtt gtgcttccac atgattttta atggtatgat
    30121 ctgcttgacc attttctttc aatacttccc gagcttcgtt ctcgattgct tcaatgcgtt
    30181 tcacacgatt ttcaagctct gtaaatttga tcattttttt ctttaattct gctgtttctt
    30241 cttccagctc caagattctt tcttgtttct tcaattgatc cgaaagcgca ttgacagcca
    30301 tcaagattgc tgcttgttca ttatccattt gtggtgataa ttgtttcaat tctgctaact
    30361 gttcattgat cagcttcgtt acgatatcca tatgatgctt ggtttcccgt ccgataatag
    30421 tatatgtttg gttggcaatc actgctttgt agcgtgtttt ttcgtgcgcc atacgaatca
    30481 tttcctccta tttgatgaaa gttttattta gtgtaccatg aagttggttc tttcaccgtt
    30541 ctccacaaat atttttcaca tacttaacta ttatatgata aactttaaaa aaatgctatg
    30601 tctcgcaatc tcttagaaaa cgaaaagtcg tgagttgcgg tatagtttta tgtataaaaa
    30661 cgaggagaat tttatgagca acgtcattat caaagcaaca aacgaccaac ttagtcaaat
    30721 gaaagcatac tatcgctctt atttgttaaa taaacagatt ccttatacta gctttgctgc
    30781 aaaaaaagac gggacgacca ttaccgctta tacatccgga aaagtgatgt ttcaaggtac
    30841 gaatgcagca atagaagctg ataaatgggg ggaggcacag gataaacaag ccaagccagc
    30901 aaaaacagcc tcttctttgc caaaaaatat cagtcaatta tccgttttag gtagtgatga
    30961 ggtcggaaat ggcagctatt ttggtccgct aacagtatgt gctgcttatg tgaaacgtga
    31021 tcagcttgca gaattacgta aacttggtgt tagagattca aaagagttaa aagatagcca
    31081 aatcatccag cttgctgaca tcttaaaaaa aaccatcccc tataaactgc ttgtcttatc
    31141 tccagaaaaa tacaatgaga tccaaccaaa atacaatgcc gttcggatga aagtcgcttt
    31201 gcacaatcaa gcgatccatt tgctgctaaa aaaaatcgag ccagaaaaac cagaagcgat
    31261 tttgatcgat cagtttacgc cggaagcaaa ttttaaaaaa tatgcgcgtt tagaaaaaaa
    31321 tcaattacaa gaaaaactgt acttcgttac taaaggagaa caatatcatt tagctgttgc
    31381 tgctgcttct atcatcagtc gtgccagctt tttggaagag ttacacaaag catcagctga
    31441 agctggaatc acccttccat caggagctgg aacgaaatct gaccaagtag cggcaaaact
    31501 attggagaaa ggcggcatgc ctatgcttga aaaatatgcc aaacttcatt ttgccaatac
    31561 agaaaaagct ttgaagctgc taaaaaaata atggaactgt gacaaaagtc atgtcacagt
    31621 cccttgttcc gaataaacgg tgttctcatg ccaactcctc ggtcacaagt cacaatagtc
    31681 atcaaacatg agaagctgtt ttttgaaatt gctccttgtg acgcggagtt aaacggcagg
    31741 ctcacaacct cttccttaaa cggtgttcaa aagcttgcat ctgcgataat aagcccggcc
    31801 aaacgaaaaa tatgaggagc tatttttgtt tggctgggct tatttattac tgaaaataag
    31861 tggtcaatgc ttgtgtcaaa cttgcagcaa tctgctgctg ataactgtct gttactaatt
    31921 cctttaaatc actatcatta ttcatataac ccatctcgat taaaatagaa ggttgaagat
    31981 tttctcgaag aacttgatag ttcccagtgg agattcctct tgaatccaga ggcagatcag
    32041 tgaaggagtc agcaattgtc tgtgctaatg tttcctcttg tccatagtaa taatatgtcg
    32101 tgattccagt tgcttcattc gcatattccg tagagtctgc gtgtaaactg ataaagacat
    32161 ctgccttttt ttcttcactg tagtcgcata tatctccaag ctgtactaat tcatctgtag
    32221 tatgggtcaa ataaaccgtt gctccagcat cctctagcgc cttttttact tttatggctg
    32281 tacttaatgt tatatctttt tcttctgttt gttcatcatt gcttaatgcc ccagtatcat
    32341 ttccgccatg cccaggatct aatacaatca ctgcatcaga tagctcttcc ggttttgttt
    32401 gtatattctt tgtgactttg gttccatctt cgagggctaa atatccagta tagccatcat
    32461 ttgtcttgat ctgataagca ttttttgttt cagaaagaaa ctccgccagc tctccgccgt
    32521 ttatagttgc aagtggggta gttagcgagt catctgtata tagtgtacgg tcattttttc
    32581 cgattttgat taatgtctct tgttctgtat catcaaatga ttcctttgtc tttgtaaccg
    32641 tactgttcgt actgctgacc gtttgtccaa ctatcttttt ttgaccaaaa acatctattt
    32701 gaaaacagat aataatgata atgattgcaa atacaaaagc tccaattatt tttttcattc
    32761 ttgactctca cctcgtagat ccagactaag ggtcaaaact taaaatcagc ttaagtacgc
    32821 aaaaaagggg ctgtaaaaga tataaccgta gaaaataaca ttactgctaa aatgattttt
    32881 ttcatttttg gcatatcctg aaatctcctt ttgactattc gtggtgattc atctacccaa
    32941 taaatagttt ccttcattct acaaacaatt aatttcttcg ttattaaatt aaaacacttc
    33001 ctggaacacc gacataataa ataaaaatat accctgataa tcatgagaga ataacaaatt
    33061 tgtatttatg aaaaactacc tatataaaaa aaacaagata taacgatctt cgtcatatct
    33121 tgcttcttca cctaactatt tttctcccta tataacagct ttacgcttct cctaatacac
    33181 cattcaaata taaatcgatc aattgcgcca catttagcgg ttcttccaca ggtcttttcg
    33241 accgagtgat gtaaggtgct aacactaaga aaaaatgcct tgctgcgatt tcttttgaaa
    33301 tcccatttct tagcacagat tggtttgctg aaaagatttc ttcaaaaggt tcacgataat
    33361 ttttcttcca tagaaagaaa agatgatctc ttgtttcctt tgaaagattg ctttcaagat
    33421 cgtgaagcat cagaaacaaa tcgaccagat gtgtctcaat caaaaactgc gccatttttg
    33481 tcaaggttac ttgaaatccc gtttcgcttg tttgaacgat ttctctcaaa gcttttccaa
    33541 catcttttaa gtggttttct aaaactgccg tatatagttt ttctttatca ccgaaataat
    33601 gatagagatt gggttgggtg atcccaactt tttgtgcgat ctctcttgtt gaagtgccga
    33661 gatatccctt ggacataaac aagtcagtcg cttctttcaa aattttttgc tgtgtatctt
    33721 tgttgtatcg atggaccatt tttccacctg ctttttggtt attctttttc tttcaataca
    33781 ccatcttcca tcataaatat tctatcgcat ttgtcaatca gccgtgtatc gtgagtaacc
    33841 atgatcgttg ctttattttt gtcttttgat tcttttgcca agatatcgac tacttctcgt
    33901 gccttttctg aatccaaact agcagttggt tcatctgcta gaataatgga cggatcattg
    33961 tacaatgcac gagcgattgc gaccctttgc cgttctccac cagatagttc ttctggatac
    34021 ttgttgatca acttatccac gccaagctgc tcaaataatt cttttgcttc tttttgacga
    34081 tgttcatgtt tgatcttatc tactaataat aactgctttt ttactgtcag aaaaggaacc
    34141 agattggaag cttgcaaaat aaagccaatt tctttaaaac gtagtttaac gcgttcactt
    34201 tctttttgat tactgaattc ttgatcattg ataaatacag agccactgct aggcgtctgc
    34261 aatccgcctg ccaaagtcaa aaaagtactt ttacctgaac cagaaggacc gatgattgca
    34321 acaaattccc ctttatcgat actcatggtt gtttctttca atgctttaac tacttggtcg
    34381 ccatcttgga aacttttttc tacttcttta aattctattg ctttcatgct cttcctcctt
    34441 cttatccaat tgccttcaac ggatcgattt ttacgattgt ccgaacagaa aatagtgcgc
    34501 ctaacacagc tactaccagc atcaacaagc taatgacgcc aaaaaacagc caattgcttt
    34561 ggaatggaac cgcatcaggt agaacaaggg atgttcccac tgttcctaat agaccaagaa
    34621 gaataccaac aaatgataaa atgaatgttt gtgccactac agaacgcgca ataaatcctc
    34681 ctgtgatccc ttgtgctttc attacaccaa agatattaat cttttgcatc gtcagtacat
    34741 aaatgaaaat cccgatcaca atagccgcaa taacgatcaa aaatccgatc ataaatccaa
    34801 aagtcaatac ttgcgcatta tatccaggta attcattgat gaatttagaa attttgatct
    34861 gttctaaatc ttcaggtaaa ttactgattt tacctctgac aactattgcg ttaatccgtg
    34921 cattttcact tgtatcttct ttttcgaagc ggatctcttg gtaagcattg atggttgtat
    34981 agagaactgg tgagacattg aatttggcat gatccgtaaa gccagttagt ttaaacgtct
    35041 tgtcactacc agataactta actgtatctc ctacggctag accatattct tctttcaaac
    35101 taatatcgcc tactgcctca tcatcgttat caaaagcttt tccttcaaca agattgggca
    35161 tgatgaattg atttttatcg ataccgaaga agctaacatt gatttttcct tcttctgtac
    35221 tgtctttact agtcacgact ccaggtgtct gggcaagata cgccactttc ccaccttcga
    35281 cttcttctgc tatcttctta gtaatcatgg acatccctag atttgaattt gcatctttcg
    35341 aaagcacaat cgaatctgcc tcccacttgt cgactgctgt tcgattatct tgagctaagc
    35401 cataagctaa cccagtcaaa aaaaatacca agtaggcgat caaaaacatc acccctgcaa
    35461 ctaacgcata acgtaacttt gaatgcataa tttcatttaa tgctaaaaac atttaactcc
    35521 tcctcctgaa ataatcattt tatacaacaa ttttatcact tgataaattt ttggcgaata
    35581 ttatgctcaa aaaattcttc cgctatttca ttatatgaat tttttattct cgattgatta
    35641 atatttatta tatccccaca caataaaaga ccgtgatatt tctcacagtc ttttatctac
    35701 tctattgcta tttgctctct tagctagtac tttaaaagct aactcattga ggtagtgaaa
    35761 acgagaggtg tgttcagaca gaaatccaaa atcctcaact gctagctgtg gaatcgtagt
    35821 tttgtgtttc acgatatccg caagcaggtt caaatctgat aataagaatt cttggcttat
    35881 cagctgtccg ccaattattt ctaatgtatc cggatggtag atcagctttg catttactgt
    35941 tttgttttct tcatcgtatt gtggaaatag atcaaattct cctgaacacg aaccggtatt
    36001 ttgttccaag aatggcgctt catcttctgt cagccctaca gttcctaaaa agtagtctat
    36061 aatgactgct gctgttatct tttgtctgag gtcgtaagcg gttggctgac cactcaaagt
    36121 tagcgctact gctcttgcca tatgtacagc tcgagtaact aaaggtaaaa agagatgccg
    36181 tttatttttg tcaaaactga ctgggacgat atctccaatc gcatagatgt ccggatcact
    36241 tgtctgcaga tattcattga ctatcacagt ctcatcctca tacaaagcca ctttttcttt
    36301 taacaatgtc gtattaggac gagtttgggc agaaaaaatg accatatctg cctttattgc
    36361 ttgagataga agttgtacgg atactacttt ctcttctgat tttttccaat cgatcaagaa
    36421 ttcatttgga taaaaattga tttgcttagt ttgcagctga ttttgaaaac tgttgatcat
    36481 atccttatcg aaatatcttg ataaaacatt cggcatataa tcaactagat gaataatgta
    36541 tcctttattt ctcaaagcat ctgctaattc caatccaata taaccagcac cgaagatcac
    36601 tacttctttt gcttctttta aagcggcaag tacttgctta gattgcacta gatttttata
    36661 ggtgtagaca ttttcagctt caatttgttt atacaaggtg acaggagagg aacctgttgc
    36721 tacgattaat ttatcatagg ttatggtctc gttttttcct tctttggatg cgaggatgag
    36781 ctgatgtttg tctggatcaa gttcagttac tctccattcg gtgatcagtt ttgccccact
    36841 ttcttgaaga gtccgcatgt tatgggcaac ttcagctaaa ctacgtatct ttccttttaa
    36901 ataaaggttg attccatttg aaacatagcc tagtccatct ttatgatctt catcgatcag
    36961 taaaacttct gctttcggtt gaagttttct tagatttagg gcagctgtga tgcctgcatg
    37021 ggaagcacca atgatcacga ttttcatgtt ttattcatcc ttaaattcaa tttttctaaa
    37081 taacattctt ttaaaaattg gttcaaatag gggaaatcat aattatattc gttgctagtg
    37141 aatacaaaaa ctttagcgga cttctgtgac gggtactctt tcaattggct agtcaaaatg
    37201 atatcatact gcttttcttt ctcgtattcg actaaatcat atttgatcgt attcagcaat
    37261 tgttcctgga tgatttcttt cacgatatcc gccattagcc tttcataagg aagatcataa
    37321 gcaatttgca gacgtaattc taactgcttg atcagcgtcc gcaaaataga tgcatagcga
    37381 gcaaataatt caaagcgggc attttcctcg tctacttcag taaaatcaac aatagcaaat
    37441 gtttgtctga ccaatctttc acataaaacc aggaattgtt tatcttctac ttcagttaac
    37501 cgctgtttta atccttcttg gccaaaaata ttcatccatc ctctaaaata ggcaaaatga
    37561 gcgtgatttt gcatcactgt caacgcaatc ttttgcgaaa tctcttctgg aaaaacataa
    37621 aaagagaaat attcttctat cgattgttta aacgttcgat tcaattgagc tactacgtcg
    37681 tctttgtatt tttcgcttat caaaaactcc tggacatgcg agttatgtgt atctaatgta
    37741 aagttactga tcatgaaaag ataaaaaacc aacgtttcat tttgattcca aggaaaggca
    37801 tattgaaaga agtaacgggt caaaaatgta tggaggtcca ataaaaattg tgtcgtttga
    37861 aacaatccaa caggaagatt gctataatct ggtttggatt ttttcgtaca gcgcattcgc
    37921 ttcttcataa tggaaagcca tatttcaaat ttcaatcgtc cttcttctgt aatagtaaaa
    37981 ctaaactctt tttgcagcag atctaaaaaa gtgtttcctg ataaatcact gtattctttt
    38041 ttgttttcaa gataaggacg tccaaaccaa aataattgga aataaaaata acaaatttga
    38101 ctttctggac cggtcagccg cccgcttttt attttcagat gaaactcatt taaagaatca
    38161 tttaattcct tcactctgcg agacaaagta gccgaactga tattaagttc catgacaagt
    38221 ttctgccgat caaatgagcc attttccaac agataaagaa gtatctgata ttttgtagaa
    38281 tagcgcaagt agcaatgata ataaacatcg actgctgcta tatttccatg tctgattaat
    38341 aaaatattcc cttcttggat aacaatttcc aattgtttga tattttccaa gttagcttgt
    38401 aaataaaaca gatcttcttt caaggtagcc cgatcaactg ataagcgggt aagtacgtta
    38461 ttaacggaaa tctctccttt ttcctctaat aaaagttgga aaagtttgat ttgccgttgt
    38521 tcattttttt caagcaaact gaaaagttcc aagtccactc ctccaagcat ttctgatttt
    38581 aacagcagaa gtttccttct catccttgaa aaagaggatc tgtgacagtt atcacagatc
    38641 catacaaatt ctaatcactt agctggttgg cccagctgtc agttcccatg tgaccatgcc
    38701 ttgatacgtt gcgccaggtt gagcattcgt actgctattt acctctaaga gtaaaccatc
    38761 ggtatcggga aagtcaacga gaacctctcc tttagctgtt ccatcactct cataaatcaa
    38821 cgtgttttga ccttgctgaa tcaattgttt tgatccagaa tgattgaagt ataattttgt
    38881 tccgcttaat tcttgacctt gatcattctt aaataaagaa gtcaatgtcc ctgtcagttg
    38941 ccaaggagac gtgcttggaa gccgagtatc ccgcacatca aaagaaaaat cgccttttgc
    39001 ttcataataa cttgtcttca acggtttggg gagatcgtta ccaaatgtaa aatcatctgg
    39061 tacattaact aatgttaatt cttgtttggg gacaagaatt gttttagcag atgtttggac
    39121 aactgggtta ggaagcttag caccatctgc taatgtaata ttgctggaaa gtacagctgc
    39181 ctttgtcagt atgctgacgg attgtacaga agtatctaca gtatttaatt gatagttgta
    39241 agtaagatga atggtatctc ctattttgct caatagatct gctgggacag aaactccttg
    39301 ttgaatgccg gtatctgcga tagttgtacc atttaatttt aaggaaccat tgatataagt
    39361 agtattttca ggaagtgaga tcgtgatttt tttgtcggtt atcgcatccc cagattttgt
    39421 taacgtgaat gtcacatttt cttgaatcgt atctcctgat agagcagaca ggggatctcc
    39481 tgtctcaggt tgtctgctaa ggttttttag ctgactatct gcactcacag atggaaaatt
    39541 agccactgtt atttgaggaa gagtggtgat tccagcttgt tcagactgcc atttgtacgc
    39601 aaaagacgga tacatgttag taagtacatc gtccaccaag cgataatcaa aataatattg
    39661 ccctgaagcc aatgcaggta gtgtagaagt agctgtattt acacccccgt tatttgattg
    39721 gatcgtttgg aaaagttgat ctgtccatcc tgtcgctaat ttttgggttc ctttcgaata
    39781 aagcctgtaa tggatcgaac ccttttttag ttcagttgct gaccaattgg ccgtgacttg
    39841 gtacgatgca gaagaaagga gctttcccca ctgactatcg gctgttaagt ttgttggtaa
    39901 aaccgttccg tctagcttgt actgctccaa tacctttaaa gcaacaggag aaagcaatcc
    39961 gccttgcgca tctttcccac tgtacacgat attggctgtc tgattcaatc cacttgtgct
    40021 tgctggagta tgagtcagta ttgctccttg tgtaacgttt tttcctgtaa aatagttgac
    40081 tacgcgatca aagcgactaa atggcaactg atcaaaacgt ccttcagtca gccccatcgt
    40141 caatgtccga ctattggtag agcctggaag gttctggtta tttccatcca cgtattctgt
    40201 ggtatcagca aatgtattat attttcctgt tgaatcactt gttgagatgt aactattcca
    40261 cgtatttatc tctgcacttg cagaaaatgc atatgctcct ggattcaagt ttccgccagt
    40321 taatttagga taaaatctac ctgcagctgg agctaaaatc gaattgaaca tacgatcttg
    40381 gataatatta ttcgaatcat aaaaatcaac agaaaaaacg gcctttttag acatagaagg
    40441 ttgtaattta cccttggtca aaattttgtt tggactcgta tcatatttat ctccgttttc
    40501 tgtcatcttc gcgacattag ctgtaacatc aaattgaagc gggatatcgt ccacgttgaa
    40561 ttcgcctcgc gtaataatag caataatatc tgccaataga gtatttgtat cccacgaccc
    40621 catcccaaaa cgcaaggtat ggcttcctgc tgataataaa ctaccgctag taaccttgat
    40681 gggggcaccg gcaatataca agtaggcgtt gttccagtca attgctttta atacatcact
    40741 gcttgataca tgttccggca aattgatgat cgccacaaat gcaaatcctt gtgcagtgat
    40801 attcaccaaa tcaagtacat ttgttagttt cacatggctt tcccaatgaa aacggaccgt
    40861 gccacctttc caaggatcat ttgaaccatc gttcaaataa gtagcagtca atttaggcaa
    40921 ttggttagcc ccagttagtt ggacaagctt cccgccattg actgcactac gtccaccatt
    40981 tggaggtacg atattttcat acgtgtatgg atcataagaa atggcaggag gcgttgctcc
    41041 aacagcttct gccgaaggtg aaaagataaa tatacttaaa acaaaaagcg aagtaaaaaa
    41101 gaaggagcga aaaaatgaaa atttattttg tagcaacatg ttcatcctcc ttttcctttc
    41161 ttctccataa caacaaccac gcaagaagcc ataattctat accaatgatc aaataaagaa
    41221 aacggatctt ttcttcattc gtctccggga atacgccatg ttgattagat tctttataaa
    41281 tcggtagctg tgtctgctgt tttgatgcgc cattgactga accacttgga tgcggaactg
    41341 gacttagatc ataaaatgta atcgtacctg aacttttggt atctgcttgg actggctctt
    41401 gccagctgaa gaacagcaaa cttgcaataa tccctcccaa taagatcgat gttttcaacg
    41461 aaatcacctc atcatgttgt ttctcctttt ttctttttct tgtggaccca caaaaacagc
    41521 aaaatcaacc ataacactag aactgcagca accaatcctg cataaagcca tgggaaagat
    41581 tgttcttgta ctgcaaaaac ggaattttca tttacttttt gtgctttttc ttttttgatc
    41641 gtaaagtttt gtgtaaatcg ccattcatta ctgtcgcctg attttgctat gatttctgcg
    41701 cggtaatcgc cggcttttgc tttatcacca gctaaatcaa gattcaagtg aaaattcgaa
    41761 tttggtgcaa ctaaaaattc attagacgta ttttcaatat aaagctgatt ttcataaaag
    41821 atcttgctcg taaatatcaa attgctgata atcctaggct gaggattttg gaaacgaagc
    41881 tgaacagttg gctttgcgtt gacctcgtcc aaatctcctg ataaaagttt cagatccggt
    41941 tctatcgtct cttgactttc ttgtaaaacg actgcgagat tataagcaat acgatttttg
    42001 atttgtgctc cttctgtagc attttctgtt tcagcttctt ctttaggtga aacatgaata
    42061 cccgctaata cacgtccctt aaagcttttt gctgggattt tcacttctat aggtatagtt
    42121 gtttctgcat gtgctgatac attgatttcc tttttcgcaa tgagcaaaac gtcacggaca
    42181 tcaaacggca atgtctcatc taacgttggg tttttttgac tataatcgat cgttccacca
    42241 tcactcgtta ccgccggatt gacagagacc tcaaatgttc gttcttcatc tgaagaattg
    42301 ctgataatta cttctagtgt ctgattttgt tccggtgcca ctaataaatc aaaataaccg
    42361 attgctttat ctatttggtt ctctggaaat accggtttga ttccaaagtc tcctgctgaa
    42421 tcctttgctt ggctgattga cggaaaaatg aaaaatagac ttaaaataga aaaaaacgaa
    42481 acaaacagtc gtttactttg attcattgtc caccacatcc tttcaacaac aactgcggta
    42541 aaaaatcctt cacctatagt attttcaccg cagtttgttg aatttatttc atactctatg
    42601 ctactgggcc agccacgagg ttccaagtca aatcagccgt gaatgttcca gctgctgctt
    42661 tcggcacttt cagtgtgata tttgctggat cataaacaga aagccatgtt cccagacctt
    42721 gatcttttgc tgctgtaaat attggtgtcg ctgcacttgc tttactcaac ccagttacag
    42781 tttgactagt tggtccagtg gattcgttat ctgacgtact tttaacagtg gatgttccaa
    42841 aatctaattc agcgccttgc aaaacttgtg aaccgtttac aaaatctgaa accgaaactg
    42901 tcacgttcca cccttgtgct tggccatctg cacctacacc tcgacgatct gatacttgga
    42961 gataaggtgt atcgtttttt gaagcgtcta cttgatacgt ttttgttccg ctttcaatct
    43021 cgtgtgtacc aaatggcaat tcaggaacta catccaatgt taaagaacct gtctggccag
    43081 ttgctggatt ttccggatct gctggctcta ctggttttga aggatcagta gggttaagag
    43141 gctcaacggt atttgttggt tcagtaaagg taatatctcc ttttgaagtc gcttgaccct
    43201 cagcaaaagc tggtgttgcc aagccgccta gaagaagtgt tgaaagtaat aatgtcccaa
    43261 tcatgttctt tttcataata aatcactcct ttttcttagt gacctaagta tagtagagac
    43321 agtatgcaaa aaatcctcat gaattcatcc gcttacttga aattatgacc ctttttgtat
    43381 aaaagagaaa aaaacaataa aatcgtttac atgaatattc ttcctttttt attaagtatg
    43441 aaaaaatatt tcaattttat tttttcgttt caaattattc tattagtgac atccttgaac
    43501 tataaaaata ggagtacggc atcatctgcc gcactcctat ttttttcgtc atttatttct
    43561 taaataaaac gattctcacg caattttatt tttttgatat cttctactgt ttgtgttcca
    43621 gctaattgca tgaccatttc caattctgtt ttaaagaaat caaacacttg atggactcct
    43681 gtacttcctc ctaaagacaa tccataaata acgggtctgc caatagcaac aagatcggca
    43741 cctgatgcga ttgctttgaa aacatgttgt ccacgtcgta caccgctgtc aaacacgatt
    43801 ggtacacgtt tatctactgc ttctgccaca tattgcaacg aatcaaacgc agcaggcccc
    43861 ccatctaatt gacggccacc atggttagat acccaaattc caccagcacc agattctaat
    43921 gatcgataga catcttcttc tgattgaaca cctttcacgt agactggcaa atcagaatga
    43981 gcagcgataa attctacatc ttttggacta agtttttgtt tggaagattt atatacagca
    44041 tccatcgttt ggcctactcc tgattgataa gcctgaacga tcggcattgg cagcgggaag
    44101 gtaaacccgt ttctgcggtc tgtttcacgg tttccgccaa ctgttgcatc cgctgtcaat
    44161 acgattgctt ttgctccatt acgcttggcc acttcaagaa tatccaaatt gattccatta
    44221 tctttgctca tataaaattg aaaccattgc ggtgctttct ccccgcctgc ttctcttatc
    44281 tcttctaaag tacaagaagc gtaagaactt gctgtataga tcgtcccaaa atctgctaca
    44341 ccttttgcag aagctttctc cgccttcaca tgagctaatc catgagcagc aactggtgcc
    44401 atgatgattg gtgcagtcag catttcttcg tcaaaatgtg tagttgtatc gggtaattct
    44461 acatctctta atacatgagg aataatcaaa cgatgattga atgcccgttc attttcttga
    44521 taagtaaata agtcgccagc accactgctg atgtaaccat aaccaccagc tggaatcact
    44581 tgtgaagccg cgatttctaa atcttccata ttgataaaat ctactattcc ttcatttgta
    44641 cctgcttgat atgttttttc catctcaatg ccttctctct ataaaatttg aatgatgaac
    44701 aatatgatag cgtttacact ttttatatta ctcttcgaca ttgatacagg caaataatct
    44761 gtttacattt cagccattcc agatgtcact actctggaag aaatgattgt aataaaaata
    44821 gataaaaaac cgatgggact ttgacaaaag tagtgtcaaa gtcccttttt ttgaataaac
    44881 ggtgttcaaa agccattttc tcggtattaa gtcaaattta tgcaaaaatg tggaaagcca
    44941 ttttcacaaa atttttctta ctactcgaaa ctaagcgact ttttcacaac ctctcgataa
    45001 aacggtgttc aatcagctga ccctcggtat tagctcaaaa aatggcaaaa tatgaggaac
    45061 tattttgcca tttgtctctc taatactcag gtcagaacgt tgacttcaca gtctcttcct
    45121 taaacggtgt tcaaaaactt gcatctgcgg taataagccc aaccaaacga aaaataggag
    45181 gagctatttt tgtttggctg ttcttattac ttgatgcagg gcaggttttt cacgacctct
    45241 gattcacggt gtttcttttt catcaacatt tttacgaaat actgtggaag actattgaaa
    45301 taagtgtgga ttgtttcttt gtgtttcaat cgtttcttgc cattcttcat ttaattttgg
    45361 attaccgttt tcttccttga actcataagg tcctaattca aaaatcccag acagtatttg
    45421 attcacgatt tcatatagtt cagtgtgatc agacacatga ttcttgaaga tcatctcatt
    45481 atcaccacgt ttgaacctca cattcgggct ctcatgatga acctctatgc atttttctcc
    45541 tttttttaac tgacggactt ctgaaaacat ctcataaata tcttctccag taaaaccttc
    45601 agcaagcgcc agactgtcca caagaccgcc tactgtcagt tcagagatta ttgcttgatt
    45661 ggaatctaat tttaatgtat agcttgtcgc ttctactacg ccacatgcat tgcaaccgcc
    45721 ttctgatagt tcgctttttc taatcaattg aacttctttc attttctctt cctcctctta
    45781 attttctact tatgtatttt tatcacctga cataggtgac tcctatcaac gctatcatgt
    45841 gcttttttcc tcagaaatcc tatttatcca ttccatctat cccttattct tctacttttc
    45901 tttctctagc atataagaga tacgcatagt attcaagcaa ggattgtcga tataaaaaga
    45961 acaaatactg ctattttgat ttcttttggc tatattacat ataaataaaa tttatcatac
    46021 ctgctctttt tgactatatc atcatctgac ttctatactt cgtttgtatt aaaacttagg
    46081 aggaatcatt catgcttaca gggttaaact tattaaataa tccttttcta aataaaggaa
    46141 cagcttttac aaaagaagaa cgggcaaaat atggaatcac tggtatgctt ccaagtactg
    46201 tccaaacact ggaacaacaa tctgttcaag cttatggtca atacttaagc aaacaaacag
    46261 atttggaaaa acgtatcttc ttaatgaatc tattcaatac gaatcggaca ttgttttata
    46321 aattgatggg cgaacattta gttgaattca tgcctgtcgt ttatgatcct gtcgttgcgg
    46381 attcaatcga acaatacaac gaaattttct tgaaaccgca agatgctgca tttctttcaa
    46441 tcgacgaacc agaaaacatc aaagcaagtt tgaagaacgc ggcagatgga cgagacattc
    46501 gtttgattgt tgtgactgat gcagaaggaa tcttaggtat gggcgactgg ggtgtgaacg
    46561 gtgttgacat cgctatcggc aaattgatgg tctatactgc tgctgctggt attaatccag
    46621 cacaagtttt gcctgtatcg atcgacgctg gtactaataa tgaagaatta ttgaataatc
    46681 ccttatactt aggcaatcgt catgctcgtg tagaaggcga aacttactat gaattcatcg
    46741 atcaattcgt tcaatcagct accgaattat tcccagaatt gttattgcat tgggaagatt
    46801 ttggacgagg aaatgctgca gcgatcttag aaaaatacga agatcaaatc actactttca
    46861 acgacgatat ccaaggtaca ggaattgttg tcctagcagg tgtattaggc ggattgaata
    46921 tatccggtga acaattgaaa gatcaaataa ttttgacatt tggtgctggt actgctggtg
    46981 tgggtatcgc aaacatcctt ttagatgaga tgatccgtca aggtgtacct gaagaagaag
    47041 cacgccaaca tttctatcaa gtagataaac aaggtttatt gtttgaagat acagaaggat
    47101 tgactcctgg tcaaatccca ttcgctcgca aacgctcaga tttcactaat gctgatgaat
    47161 tgacaaactt agaagcagtt gtaaaagcga tccatccgac aatcatgatt ggtacttcta
    47221 cccaacctgg agcattcact gaagaaatcg tgaaagaaat ggctgctcat acaccacgtc
    47281 cgatcatcat gcctttgtct aatccaacaa aattggcaga agcaaaagca aaagatttaa
    47341 tagaatggac ggacggaaaa gcattagttg gaacaggtat ccctgctgct gatgttgaat
    47401 ataatggtgt aacttaccaa atcggtcaag caaataatgc tttgatgtac ccaggtttag
    47461 gacttggttt gattgcttca acagctacac gagtaaatgc tgaaatcatt tctcaagcta
    47521 gtcgagcatt aggcggaatc gttgatgtaa caaaaccagg agcagctatc ttgcctccag
    47581 tagcaaaaat cactgaattt tctcaaacga ttgctgaaac tgtcgcaaaa tcagttgtcg
    47641 cacaaaactt gaatcgtgaa gaaatcacgg atatcaaaga agcagttgaa tctgctaaat
    47701 gggttcctga atataaatct ttagaagatt aagatcgtta tagtaaaaga acatacagaa
    47761 actttgtatg acgtctaaaa agaaaaaagc gactgcggca aaacagcggc cagccaactc
    47821 ctgctctttt gtcgcttttc tttggctttt ttcatatgat acagaataag aaaggatctg
    47881 taaaaatggc acaaaaaaca acaactgcaa ctgcagttga ggaaaagaaa aaagaaggtt
    47941 tttggtccac aaaaatcacc ggcgtaagtt tgccacttta tttgatcatg gtccttgtcc
    48001 tgatcattgc aatggcttta gggaaattac cgacgaatat gattggacca atcgcagttt
    48061 tggttatttt cggtaattta tttcatttta tcgggacaaa aatccctatc gtcaaaagtt
    48121 atttgggtgg tggttccgtt tttgcgatct tcgcttctgc tgcgattgct acatttggga
    48181 tcttacctga atatgtagtg aaatccacag aaaactttgt cagcaatatg ggcttttggg
    48241 acttttatat tgctgccttg attactggta gcatccttgg tatgaaccgt aatctattga
    48301 tgaaagcttc tgttcgtttt attcccgttg cacttatttc aatggtcgtc tccttttttg
    48361 ccgtaggact cgtgggcatg cttatcggaa atggttttgc aaattccgtt ttatacatct
    48421 cattacctgc gatggctggt ggtgttggag ccggagccgt tcctttatca acgatttatg
    48481 caaatgtttt aggtacggat gcttcttcta tcatttcccg cctgattcct gcgacagcaa
    48541 tgacaaatgt cttagctatc atcggtgcag cattagttgc tcgtgctgga caatcaatgc
    48601 caaaattcaa cggcaatggt aaattaatgg aaaaaggcga tcttggcgat ggaaaacctc
    48661 gaactgtgaa accttccatt ccacagttag gtgttggttt gatggtatca ttgactttct
    48721 tcatcttagg acaaattggt aactattttg ttcctaaagt ccatgcttac gcatttatga
    48781 ttattatcgt tgtcatctgt aaaatcgcca acctattacc agagtattat gaagatgcag
    48841 ctatcatgtt caataacttg attgtcaaaa acttgacagc agctgttctt gccggtatcg
    48901 gtattgcgtt gctgaatctg aatgtattag cttctgcttt gacatggcaa tttgttgtct
    48961 tatgtctgac aagtgttatc gcgatttcag ttgtttcagg atttgtcggt cgtttgtttg
    49021 gtttatatcc aattgaatca actattactg ctggcttatg caacaactcg atgggcggaa
    49081 caggaaatgt agccgtctta tctgcagcta atcggatgga acttattgct tttgctcaaa
    49141 tgggcaatcg acttggtggt gccatcgttt tgatcatctc tggtttcttg atgcaattac
    49201 tttcttagtc aaaaaaacaa aaatacctga tcatcaagta tcggcttggt gatcgggtat
    49261 ttttttgata aagatttaca ggattttcaa aaatcttttt tgcattttat cggaatgata
    49321 accgatgtgt tcataaaatt ggtgtgcttc tactctgtat tctgcagaat taagccttat
    49381 tgtcgttatt cctcgttttt tcgcttcttc ttctacgcca gtcatcaaag cctttccgat
    49441 gcctcttttt tcagcatatt tagacacagc aagtgccata atattgaaca ttggatcgct
    49501 aaaaatcgat tcataggact ccgcttgtac atagccagct gcttttttag aagtaggatc
    49561 ttcataaacc aagaaaaaat ttttctctgg ctccttcagc agtttcttca tttgtctcac
    49621 cgtaacggtt aatggtatat cataccctaa ttgatccgca ttgatttcac gtagtgcttc
    49681 tgcatcttct aaacgcattt cccgtaacat ccaaatcacc agccaatttc tgatttttta
    49741 aaatgaattt ttttatcata gtatactctt tttttcattt agaacaatat gtttcagtga
    49801 ttttgacttg cgttagtcta gattctccta atgtttgtaa gatgacaaag gcattgtaac
    49861 aattgaccaa aagaaccacg aggacaatta cttgaaaaat aaaggtcact ttttttggag
    49921 acaaaaccct gctaaatctt gctccccaca aacctccaat aacagcagcg gggataacat
    49981 aaaacaacat agataaatca aagcgcataa agctacttgt taaagcaatc gttaccaatt
    50041 ttgccagttg agaaaagaaa atcgtagata gagaatatag tgttgcttct tttattggca
    50101 atgcaaacat cagcatcaac aaagaaacat tgactggtcc accaccaata cctaaaaaac
    50161 tagctaaaaa gcccagaacc ccccccacat ataaggtacc aagtccatga cgtcaaatga
    50221 aattttggct gatgatgctt ggtgtagaag aacgcaaaaa ccaaggtcaa taccgtcaaa
    50281 aaaatttgga tcagctgcac atgcttctcg ttttcaaata actgtaaaaa aacttcgaat
    50341 acaatgttgc cgactactcc tccaacaaca gctccactag atacccaacc gacaatctgc
    50401 caattcagtg atttcccgct tgatacttgt cgcacagtcg aaacaatgga catcgtgaat
    50461 accgcaactg ttgagtaaaa agaaatccct gcaacagaat gggcgccgat aagatcaaga
    50521 atcggtttga tcaacacgcc tccacccatc ccggaaatag cgccaactgt gttcgccaga
    50581 acgatcacca caaaataaat cagaccaatc atcttgtatt tcctttctct tgtttatatc
    50641 ttttagcgat tgttactctt ttcttttatt atacagtaaa agaaacaaac gaggcttcga
    50701 aagcttttct actttttaag gaaatttagt gctaaacgaa aagaggttgt gaaaaagctg
    50761 tcctgcatca agtaataaga acagccaaac aaaaatagct cctcataatt ttcgtttggt
    50821 tgaacttatt accgcagatg caagcttttg aacaccgttt aagaaagagg tcgtgagcct
    50881 gccgttcagc tccgagtcac aaggaacaat ttcaaaaaac agcttctcat gtttgttgaa
    50941 tattgtgact tgtgaccgag gagttggcag gagaacaccg tttaaggaag agactgtgaa
    51001 gtcaacgttt tgacctgatt attagagaaa caaatggcaa aatagttctt catatttcgc
    51061 cgtttttcga gcgaatgccg aggggcagct gattgaacat cgtttatttg gaaaaaggga
    51121 ttgtgacaag acgtttgtca caatcccttt ttttatatct tattaattca ttcttttgtt
    51181 ctattcttct tcttccgcca tgaatgtatt gaatacttct tcaatcatgt cccattctgc
    51241 gtctgtttcg atttgttcta actcgccttc tgttccatct tcgttttcga tgtatgcata
    51301 agcttgtaat tctacatctt catcttctga aacaccagca ggatataaaa gaacatagtt
    51361 acgtccaaat tcttcttgtc cgtcaactgt tagtaggatt tcatataatg tttcatttcc
    51421 ttggtcatcc accaatgtga tgtgttcgtg tccttcatgg tcgtgggaat ggttatgttc
    51481 tgtcataaga ttacctcttt tcttttatta ttaaaataat tgcttacctg agccgtctaa
    51541 ataattttgt aagatcatca cagctgctaa tttatcgatg actttttttc gtttggctct
    51601 tgaagtattt gcctgctcta ctagcatacg ttctgcctga acagtggtta atcgttcatc
    51661 ttgataatca actggcaagg agaatagttc tttgatcttt tctccgtatg acatggaagc
    51721 ttctgccctt ggtccgatgg aattgttcat atttttaggt aatccaacta caaaccggtc
    51781 tacttcatat tgtgctacta attccttcaa ccgatcaaag ccaaactctt cttctgcttc
    51841 attgatccga atgatctcta ctccttgagc tgtccagcca aaggggtcac tgatagcaac
    51901 acctactgtc ttagaaccga catccaagcc cattactctc ataaattcac tccatgattt
    51961 gagaggtagt ccttgacaat tacttccatg atctcatcgc gttcgtgacg gcgaatcaaa
    52021 tttcgagcat cacgataacg aggaatgtag gccgggtctc cagaaagcaa gtagccgaca
    52081 atctgattga ttggattgta gcctttttct tccaaagccc gataaacaat cgctaatgtt
    52141 tcgctcactt cttttttgcg actatcgtca aaaccaaaac gtacggtttc atctgtaaaa
    52201 cccatgattc tacacctctt tcccgcaagg taattccagc ttaccttcta caaccttatt
    52261 ttactcgaag aaagggaaaa ctacaaacaa atattccaaa cttccgaata aattaagaaa
    52321 cactatttgt atgttttttt ccttttttct gtagctttat caaaagtact cttttcataa
    52381 agtatacgct atatttcttt gaatgtatgt ttttacacaa ttttttcata atattcataa
    52441 aaaattcgaa tagctgctac taattttttg tttttgttgc ttgtcctgtc tcataattga
    52501 tcgtgtagcc atcttgaaca ttgtagataa agacattgaa ttcaattttt ttgtcttcaa
    52561 tgctttctgc ttccaattgc actcccctgg ctaccagttc attcccctca aaatgaggag
    52621 tgacacggta gcgtacatga tgatttgttt ctttgatata tgccgcaata tcattttcgt
    52681 gagcaagcat gtatggagca ttcaacgaac gtgtccctgt catcaagttt ttaatgttgt
    52741 tattttgacc agtcagctgg aaaccaatta aatggctgcg attatataac cattgcccgt
    52801 cacttaattt cttttgtttc caacctgtcg ggtcaatata caaaggctct ctcttttcag
    52861 tagggaacaa atcttttcca agcatagcat tggcttgtcc tactctgttc aatttatcaa
    52921 tatttgaata gctttcccat gttcctttgg aaaggctaag atcctctttt gtaaattccg
    52981 gctgattccc attgatttct atttcttgat gttttccatc atattctggc acatccgcta
    53041 cttgagaagc ttgtggtgtg gaatcaaaaa tagttgtaat ctgctgaaag atatctgaat
    53101 ctttttgtgt ggtggaaaca ccgaatgccc ctgcaatcac agcagcgacg atccccacga
    53161 tcgccttggt tgtttttttg ctcatttctt tactccttta ctaacagctc cctaaatctt
    53221 aacatatgtt cccctataaa taaatactag aaagaaatag aaacagcaaa aaataaaacc
    53281 tgttttaaga tggcttaaaa atacaaaaaa gagacggatt cctcgtctct aatcagtcac
    53341 cactctatct gtaaggtatt tattattatt caaaaatttt cttcatactc aagacaatac
    53401 actgatttca caaccgcttg gtaaacggtg cgcaaaagct tgcatctgcg gtaataagtc
    53461 catccaagtt aaaaacataa gaagttgttt ttgcttggct gttcttatta cttgttgcag
    53521 agcagctttt tcacaaccta ttttaagcgg gtgacgggaa tcgaacccgc gacatcagct
    53581 tgggaagctg ggattttacc actaaactac acccgcaata caaaaacagt atacgcctct
    53641 tttattcctt cctcaagttt ttttattctt tacgcttctg gtatgttttg tagtaaaata
    53701 aaactaatca atcaattatt ggagaagatg ctatgacggg aaaagaatta gatctgttgc
    53761 tggacacttg tttactcgct gggaaaatca tgatggaaag caatgcggag atgtatcggg
    53821 tagaagatac gatgagtcga attgctttag cttctggaaa tttccgtttg gtcagctatg
    53881 tcactcaaac cggtctcttt attggtcttg atcgtacctc gaccatccga atggaacaaa
    53941 ttaccaaccg ttcgattaat ttagaaaaag tagtaaaagt aaatgatctt tcacgtaaat
    54001 atgttgcttc agagctaaca ttagaagaac tttactgcga actgcaagca atcgaccgag
    54061 accgttcttt ctttcctgtc tggttgcaaa tcgttagcgc ggcagtgatc agtggaacaa
    54121 ttatggtgtt atttggcgga aagatgaccg atttacctgt gactttagtc attggaggat
    54181 tgggatacgc agcttatcta tatagtttga aattttttag aatcaaattt ttatcggaat
    54241 ttttatcttc tttattgatt ggcatagccg ctatctacag cgtacgtttt ggcttaggaa
    54301 gcaatcagga tctaatcatt atcggatgtg tgatgccttt agttcctgga gttcaaatta
    54361 cgaacgctat ccgtgatttg ctagctggac attatgtctc aggagtttct cgcggaacag
    54421 aagcgatgat gactgctacg atgatcggtt ttgcgattgc ttttattttc cagctttttt
    54481 attaggaaag gaacacaagt atggggacga tatttattca gttttctttt agttttttag
    54541 cgactgcggc atttgccgtt attacaaatg tacctaggcg ttcattgatt tgttgtggtc
    54601 tttctggtgc ttttggctgg atgatctact ggatctgtgt tcaaataggc ggatctgctg
    54661 cttttggttc attgcttggt tctttaggag tagcattcat cagcgatctt ttctctaaac
    54721 gtttgaaaat gcccgtaacg attttcaata ttcccgggat ggttcctcta gtccctggtg
    54781 gtttagctta tcaagcggtt cgaaatctcg ttactgaatc ctatcgagaa gcggctcatt
    54841 atgcggtcca agcaattatg attgctggag caatcgcttt aggtcttgta ttatcagaag
    54901 tctttaacca taacatccgt aatttcaaag taaagcgtga cgcaatcatg atgaaacgaa
    54961 aaaaacggct ttttaaataa acgaagtgta acaaagaaaa ggttgtgaaa gagacgttca
    55021 gccttgagcg atgacaaaat atctcacaaa cattgcgaca agtaaccgca ggagcaataa
    55081 gaagtaatta tgagtattaa aaaaagtttt gtaaaaatag cttctcacat ttttacataa
    55141 aattgactta atactgagaa actgactttt gaacaccctc tatctgtaaa aaaggggctg
    55201 cgacatgatt tttgtcacgg cccctcttct ttattcaaat tttttccaaa acgttcgttg
    55261 ataataaacg atccatagag cggaaagtat tagaactaaa ccgatcacag gaatattcac
    55321 taaccaaaat ggataaaaca aagccaacat tccgtataag acgataagaa taatactgat
    55381 gatcaatgaa cccatcatag ttgcgactaa acccaaactt cctgaacctc tgttaaataa
    55441 ttggttaata ttggtccagt tcagcagcag caaccgataa tctctagcga aataacgtag
    55501 tgacaataag tagcagccga ataaagaacc aaatagcaag ctagggataa aaaacaaagg
    55561 cagctgaaaa gacagtccgc ttaataatgc gatcccacca gttaaaaaac tctgcaatat
    55621 ccatccgaat ctgaactttt cttttaaata ttggttcata gagattggca aagaacggat
    55681 aaacagaaaa ttttcttgat ccaaagaaat aagattgctg ataaaagaag tctgattaac
    55741 tgttaagaaa gccaatgcga tccctaccag gaaaacaaca ccgattaact tattatctaa
    55801 atcagttaat tttatttcgc ctcctagggc aaaagcaaga ataaaaatga ttggagttaa
    55861 caaggaagaa gaaagaactt gcataatcaa attaggttct tttactaatt gagcattgta
    55921 actaaataaa agctgatgca cattttggtt ctttttatgg gttcttttta ttttgaactc
    55981 actgtttgaa tcagtcagtt gctctgtaag ctttggtact atcagctgtt ttacagcaag
    56041 gaaaaatccc agagccgtga tagctagtat tccccaaccc aataaacctt gttgagaaag
    56101 tggctgactg gcagcatcat agaatggcaa taatatcgaa atcggatgcc gatctgttaa
    56161 ctcattttca atagtccccg tttgatgatt catccacatg attccagcaa atgcgatcag
    56221 catcgagctg ccaagcaaga tactagaaac tgtttttcta tgttttttaa acactgctgt
    56281 tcgagccaaa ccaaaaacaa tcaaactagc cagacaaaaa agaataatca aaaatagaga
    56341 aaacaaaagg attgagataa tgatagtaag caaaatccct agatcagctc gccatccggt
    56401 taaaataaac atgactagaa gcggaaaaac aaaaggaatg atggtcaaag caactaccaa
    56461 aatctttgct gaaaaaacct gttcttgtcc aaaaggcaga ggcaaaaata cttgcaggtc
    56521 atttccttca aaaaagatat tataaatgac tgatatactt tgagttaaac ctaacatacc
    56581 aaaaagtgcc acgtaataag taaagaaacc tggcaattta acaaagttca tagcaaacat
    56641 agcaaatccg taaatcagta aaaaaatcag ccctgaaaaa agattttgag acaaaagata
    56701 gatagttaat cgctttccct tttttccttt ctctcttgct ttattcgtga gctgtggatt
    56761 aacatataaa agattaactc gaattaattc taataattgt cgcttataca tacggtccat
    56821 ctcctgtcgc ttgccttcca gccattttca aataaatctt ttctagcgtt tcatcaggtg
    56881 agccattcaa taattcacta actgccccct gataaatcaa ttcacctttc ttcaaaatag
    56941 ccagtttatc gcataactgt tgtgcggtat ccaatacatg tgtagaaaaa atgactgttt
    57001 ttccttttgc ggcatgagcc ttcatcattt ctttcaaatc aaatgctgct tgaggatcaa
    57061 gtccttgcaa aggttcatcc aatacccaaa tatctggatc tggcagcaac gtgccgatca
    57121 aaatcgtttt ttgacgcatt ccatgtgaaa aacttgcgat tgtttcattt tgatgactat
    57181 acatatcaaa aagcgctgtc aattctgcta aacgcttttc ttttttttgt gtgtctaatt
    57241 cataggcagc tgaaatcaag tcccaatatt ctccagccgt caattgtaaa aaaatatctg
    57301 gggaatcagg cacataaccg attttttgtt tgatactcaa tcgattttcc gtcaatttca
    57361 ttccatctac taaaatcgtt cccgatgatg gttcaataat actaactaaa cttttaatcg
    57421 tagttgattt tccggcacca ttatgtccta aaaatccaaa aatttcccct tgtcttatcg
    57481 agagagacaa gtcttttaat gcttgttttt tcccgtaact tttacttaca ttgatgaatt
    57541 caatcatttc tgtactcctt ttgtttgtta ttacttttta ttgtaccctt cgttattggt
    57601 ggagtaaatg tcaaaaagag tgcataacaa aaaaggatag aaacgactag gctgctactt
    57661 gggttgttct cttctacagg aaaacactct tctaacaggt aagatatgag tctttgtttt
    57721 ctttaaaatg attcattttc tatcttttct tgcataaaat tctttagttt atcaatactt
    57781 ctctttccca tctctagttg ttctccattt gcaaatgtta ctatttcgtt tactctgcta
    57841 atagatgtaa tgttttcagt attaatgata taagctttta aatctttaca taaatactca
    57901 tattcgagtg attttttata ttttttaatt gtgccgttta cgagaatctc ttcatctttc
    57961 aatactaggc ctattttatt tttataacct ggcatgctca ttagataaag gatttcatcg
    58021 taatggatat agtgatcgct attaccataa cggacagttg tccacctttt tttcgtagca
    58081 tgttgaacag gatagttttc tttttctttc aataagtaac tttggatgct ttttttattt
    58141 ttttcataag tgaattcttt tatcaaataa ccactaggcg taatctttcg attaataaca
    58201 gttattgctt tattcggatg agacgttaga taaacaattt tacacttttc attcttttct
    58261 ctaagcattt cgcctagttc gataccgcta taataggcgt tcaaatcgat gtctaggaaa
    58321 aaaatatctg aggaatagat ttcattattt ttaatgaatt caacaaattg tataacgttc
    58381 aatccctgaa cgatctcaaa tttccaagga cttaataaaa ttctggattc ttccaaaatt
    58441 tctactagca ttttttcaaa aaccaaacta tcatccaaaa taaatatacg cgttaccata
    58501 aaaagtacct ccactcgata ttttactatc aaaatcattt catacaactt ttcaacatac
    58561 atgttaggaa taaattgtaa ttctcttcac aatcttttcc aacacctttg aaaccgatat
    58621 caaatacttt caggatgctg cttctaaaat aaatatcaaa caaatgaata catcatgcaa
    58681 aaaaagccag acaaccgacg tccttgcgtg tcagctgtct gagctatgtt tatttatttt
    58741 gttcttttaa aaagaacgat acacatactg atttttcgga gcagagactt tctcagcgtt
    58801 tgtaacaact acagatggga tttcacgctt gaatggttga taatagtctg attggatggt
    58861 tccttctatt ttataccatt catcattttt aaccgttact ccttccggca tatgcgtcaa
    58921 caaaccaaag actcctgaat ctgccacaca atggataatg ccgaatcgga acaagaagat
    58981 atctgtattg ctatcgttcg gtgtttggta aacaaatcct tcatagctga tttttttgcc
    59041 aatgaattcg ctagggtaat tataaattag ctccataact tctaaatagt tttcatctgt
    59101 gatcttgata gtttgcttat ccccatactt ttctttcaac tcattcattt ggtctaagta
    59161 gtctgatttg ttgaaataaa tactggtatc cggctttaag tattgtgtct gcatctccgg
    59221 atcccctact gattctttac ttactgggaa attaaatcct ttggcttcta cgatcgtagt
    59281 gtcgagacta actgttggaa acaacgtccc cacgattact ggcaaagcta gcagtacata
    59341 agcgattcca cgttgataag gttttgacaa cccatggtca tgatcgtgct cgtgtgcctt
    59401 ttcttctgct tttccgtttt tgacccacaa atacagctga actaaagcca aaagagcaga
    59461 taaaatcatt gaaagaatag ctaagtagcg ataatggaca ttgatatatt gatcgagttt
    59521 gccagaaact tgcaagtaca tcatcaattc gaaataccca gataaaatca aaaaccgcag
    59581 catattttcc ctcctaaata aataatgtga atccaataac gctaagggca atcagtccga
    59641 tcagcgataa aataaagcgt cctgtaaaat atcgtttcat catcagtaga tttttgatat
    59701 cgaccattgg gccaaagacc aaaaaggcag ctacaggtgc tgtgccgaac agacttaaca
    59761 aagaagatcc tataaacgca tctgcttctg agcaaagaga catcgtaaag gccaataaca
    59821 gcatgatgat aatggctaaa atctttgttg agccgagcgt tagcataatt ctagttggta
    59881 aataagtctg catcgcagct gcgattaatc cgccaatgat caaataacga cctgtatcaa
    59941 aaaattcgtc gattgaatgt gtcaacacat gcccagtttg atggatcagc tgattttttg
    60001 gttcattctt ctcactttgt tcttcttcat ggacatgatg atggtgattt tctgctactt
    60061 tagacgtgat tttttctttc aatacagcgt ctttttgaaa ataggcaagc cagacaccaa
    60121 tcacaagggc aacaaaaaag ctgcctatca cccgccagct tgccatctgc caagaattgc
    60181 caaatgcgat aaaagtggaa aaaatcacga ttggattgat gatcggagca gtcagcataa
    60241 aagcaaacgc tgtgtgaact ggtacgcctt ttttaacaaa ttgatgaacg atcggtacga
    60301 tgccgcattc acatgaagga aagaaaaatc ctaagatcga accagtaaaa atagagaaga
    60361 tacgattttc aggaagccat ctcttcacac gttctggggt caaaaatact tgtaaggcgc
    60421 ctgagataag acagcctaat aatacgaaag gtaatgcttc aataacgatc gataagaaaa
    60481 tcgtactcat ctgcaaaacc gaatttggaa taaaagaaaa catgagtttc ctccttttta
    60541 tttctagttc attcgctttg caaatatacg cttttattca aaaaaaacaa gttgctctgt
    60601 aaatttttac acaactctta ttttttacca aagcacttca attgactttt tctgttattc
    60661 ccattaaatt aaggtctcat acatatcact tctatcataa aagggagaat aaaaatgaaa
    60721 caaaccacta acttgactga agttcaagac atactgcaac agtcatccgt tgccgtcatt
    60781 tatctttcga tgcctaattg cagtgtttgt cacgctgttc gtccacgact agaggaactt
    60841 ctcacacatt atgatattcc ggcacttcat ttggatgcat ttgaaacacc agaagtagct
    60901 agtaaatttg aagtgctgac tgcgccagtt gtcctgattt ttcatcaagg aaaagaagtc
    60961 tttcggcaag cacggttcat cgactttaaa aaaatccgtt atctactaga tgaactgaca
    61021 gcagaagatg attccctttc ttatgaagaa atcttccgaa caaaataatc gttgtccaaa
    61081 cagaaaaaag cgattgaaca agtgaaagtt ttgccttcat ttgttcaatc gctttttatt
    61141 tttattctgc tgtattttcg gaatgcgcat ttgcgtcaga atgcaagaaa tagatcaagg
    61201 tttgcaattc attcgtcaaa tcaacatttt ggatcaatac atctgacgga gcattcaatc
    61261 gagcagcggt aaagttcaag attcctttga ttcctgcttc cgttaactga ttgactactt
    61321 cttgtgctct ttcagcgggt atcgttaaaa ttgctacttc gatttgttgg atcttgattt
    61381 gttctgacat atcttccatt ggataaacgg ggataccgtc tacgatgcgc ccaacgattt
    61441 cctcattcac atcaaaagca caactgatac gtatgctatt actttgatgg aatttgtatt
    61501 ttagtaaagc acttcccaag ttccctacac cgatcagcgc aacatttgtc aattgatcgt
    61561 cgtttaatgt ctttgcaaaa aagtgcataa gatcttctac atcataacca tacccacgtt
    61621 ttcctaattc gccaaaataa gaaaaatctc gacggatcgt tgcactatct acttgaacgg
    61681 cttcacttaa ttcagttgaa gacacttttt tctttccagc attatttaaa atccttaaat
    61741 agcgataata caaaggcagt cttttagctg tcgcctttgg aataatatga tctttcacga
    61801 aatgacctcc acgtattgtc taattacatc tttgatttct ccatcatagc agtatttata
    61861 ggaaaaaatc aattccattg ctcagatatt gtgaaatttt attctttatt catttattta
    61921 tcatttctgc aaatctcgca gtataatgga atttatggta gactactaat tgaaaaatag
    61981 ttttttattt gaggagaatt cacaaagtag aaaagaggat tccatgatat tattacaagc
    62041 caatcagatt gctcgccatt ttggtgcaga agtcttattt gaaaacatac atttagaaat
    62101 cagtacaggc gctcgtgtcg ctttagttgg cagaaatggc gctggaaaat cgactttatt
    62161 aaagatcatt gctggaattg aagcacctga tgaaggaaag atcgctaaaa ataaaacagc
    62221 gactctcgga tacctcgctc aggacacagg acttgattcc aatgaaacag tttggaacga
    62281 gatgctgaaa gcttttgaac aagttagaca aatggaagag cggatgcgcg agctagagat
    62341 tttcattagc gaaggtacgc cagacacacc tgcttatagt tcttatttaa aagaatacga
    62401 ccggctgcaa cacgattttt cagatcgaaa cggctatggt tatgaaaatg aaattcgttc
    62461 cgtattgcat gggtttaaat tcgattcttc attttatgat cagaaaatcg atactttatc
    62521 aggcggacag cgcacacgtt tagctttagc tcgcatgctt ttgcaacacc ctgacatttt
    62581 gattctagat gaaccaacga accatttaga tatcgagaca ctttcctggc ttgagaatta
    62641 tctgcaaagc tatactggtg cattattgat tgtttcccat gaccgttatt ttttagataa
    62701 agtagtcaat gaagtctatg aattaagcag aagaaaaatg cgccattaca aaggtaacta
    62761 ttcgaaatat ctagatttga aagcagaaca gttagcaagt gaatggaagg cttatgaaaa
    62821 gcagcaaaca gaaatccata aattagaaga cttcgttgct cggaaccttg ttcgcgcctc
    62881 taccacaaaa cgagcgcaaa gccgcagaaa gacattagaa aaaatggacc ggctggatcg
    62941 tcctcaaggg gatgaaaaac aagcaaactt tctattcagt atagaaaagc cttctggaaa
    63001 tgtagtcttg caaatcgaag atgccgctat cggatatgat aatgtcacct tatctgaacc
    63061 aatcaatcta gatatccgtc gtcaagaagc gattgctcta gtaggtccga atggtatcgg
    63121 gaaatcgacg ttgctgaaat caatcattca ccagatccct tttatccgtg gaaaagaaac
    63181 gctagggaca aatgtcacca tcggctatta cgatcaaggt caagctgatc tgcatacaaa
    63241 caagacgatc ctagcagaat tgtgggatga gcatcctacc actccagaaa aagagatccg
    63301 aagtgcttta ggtgctttct tattttctgg cgaagatgta gaaaaaccaa tccctctgct
    63361 aagtggcggt gaaaaagcac gggttgcttt agccaagtta gcgatgaaca aagaaaattt
    63421 tttgattttg gatgaaccaa caaaccattt agatattgac aacaaagaag tattagaaaa
    63481 tgcattgatc gattatgacg gcacactgct ttttgtttcc catgaccgat atttcatcaa
    63541 tcggatcgcg acaaaagtcg tcgaactctc tgaaaatggc agcaaagtct atctaggaga
    63601 ctatgattat tatttagaga aaaagcagga agaagaagaa atcgccgaat tgttggcaca
    63661 agatgagcca gaatctgctc ctgctcccac gaacaaatct gattattacc aaagcaaaga
    63721 acagcaaaag cttatccgta gccttcaacg aaaaattact caggttgaag aagaaatggc
    63781 gcggatcgat gaatggatcg atcagttgga acaagaaatg gtcgcgccag aaaacctgga
    63841 agatcacgta aaactcaatg agctgaacca agatttagaa gctgctagac aagatcaaga
    63901 aacaaaattg acggaatggg aagaactaag cttggaatta gaagaatatg aaaattaacg
    63961 aaaaaaccaa aacagaaacc attcatgctg ttttggtttt tcttttttta ttttatcaaa
    64021 caaaaattcc atatttacct ttttcaccaa taagaagacc aattactagt cattctcact
    64081 aaagcttcta aataatagta atcaccccaa atacaaggtg tatttacccc attatttttg
    64141 ggcttattat aaacagactg tgttaaaaag ccatcttctt cttccgttgt agcatatctt
    64201 tctttcaatt gtcgcataat tttccaaaca gcagactggt ataagttctt ttctgttttg
    64261 tctaatagac ctgattctat taattctaat aatccacaaa ccatgatcac catactagat
    64321 gtatcttttt cttctctgct agaatcatca aagatcaaat cccaataaca aatggtatct
    64381 tctggcaacc gattcataaa ataattggct aatattttgc ttaaatcaaa atatttttgt
    64441 tctttcaaat aacgataact taatgcaaaa ccgtaaatcc cccatgcttg gcctctagcc
    64501 caacaggaat tatcactata accttgatgc gtacttccat atcttggttt tccatctttg
    64561 acatccatat aaaatgtatg ataagttgaa ccatcttctc ttactaaata atctgctgca
    64621 ttttgaatat gagcctgagc agctttttta tatttttgat ttccagtttc ttcactcgcc
    64681 caatacaata atggaagatt catgcaacaa tccatgatca ttctaccttg ttgtgaagga
    64741 tcactaggat ctccccaagc ttgtataact cctgccttct cccaaaaacg ctgaagtaat
    64801 gtttctgcag catcaattga agcttccttc atttttcgat ttcccgttat tttataatct
    64861 gctacggttg ataaagaata caaaaaacca agatcgtgat gattagtgat aatttgacag
    64921 tcaatcctgt ggataaaatc tatatctgct ttttcagctg tttgttgaaa taaaggatca
    64981 cccgtcttct caaataacaa ccaattaatt cctgtccaaa atccttctgt ccaaccaata
    65041 ttgtctctta atggataacg tccttttata ggacactcgc ttgggaattg atactgactg
    65101 ttactgatac gtttgattaa cctctccgtg tgtctgcgac aacgctcgat ctcattattc
    65161 cattcttttt ccattaattc tttataacat tcatttatcc atttttctgg atgtattatc
    65221 tcttcaaaca tcactttatc aagcatttcc ccaccagcct ttagcataat tttagtcttt
    65281 ttttattgtt tttattttgg atagttagcc cggttgtccc atcaaaatca attttgggac
    65341 gattgttcca tacttctaaa ctataatccc gatcttgatg agcaaccact cctgttccga
    65401 gccaatgggt tccttttcca aatgtaccct ctaatccagg aataaaagtg ttttcccatg
    65461 tcatcaagtt tgtattgggc aaacccataa tagctgtgga ttttcgttta atagtatcgt
    65521 cacttaaaca gataatacct gaaaagccag cttcactcac cctatagttt tccttgtctt
    65581 cttctatgat tattccttct ttctcaggag aacattcaat agcaaaccct gtctcatata
    65641 gaacatattc tgtatctaat tctactttgt gtacgcgaat ctgccagcct tcaaaaggaa
    65701 taagccaagt tctgattttt gcctgtaaag taggttccca tgtagaggca agtacctctt
    65761 tcttacatac aatgtctcgt acattatttc gaacgatgaa ctgtccatag gttttgatgc
    65821 tctcattctt aaaactggtc gtgatatatg tgtccgctcg ctgaatccct agcattgaat
    65881 ccattgccat ttctgctttc aattgattcg ctcgcggtat cgaaaaacca aattgactag
    65941 aataagcaaa ttttaaatat ttttcattgg tcaatgtatg gaaatttggc cctaattgtc
    66001 ccgcattcaa aaaaaatgta tgtccattat catgttgtat gagcaaattg gcttctgaaa
    66061 gaagtttagt tccttcacga ataggcatag gcatttcttt agccgtccaa tactcatggt
    66121 cttcaggtag cgccaacaat aaaaaaatct tattcagcca taatggagat aacgtactat
    66181 tatagggttc agtcaatgtc aattgagggt aggtataacc aatattcagg ataccttttt
    66241 catcaaatat ttctttttgc atccaccatc ttatattccg attgactaac cccttgattt
    66301 cacctaattt aaaaggcaaa agatcggccc acaacatagc tgaccaaaag cttgaaactg
    66361 caaaacgata aatcatgctt cgaccaaaag gcacatttgc accatcttcc gcaaaaaaat
    66421 gaatatagtc tctagaaaaa tcaatagctc ggttcttata tatctggcta atgggctttt
    66481 ctggttctag tacgctataa atcaaaccat aataatgaaa tgcaaaagga ttataataat
    66541 cttggcgacc tcttgaactg tctcgatacc agccattttg aagataacaa ttttctatat
    66601 tatcaaatgc ttctttcagt gctgattctt taactggata accaaatttt tggataattg
    66661 ttccgacaat tatcttaaaa aattgccaat taccatctgc acattcatat tcaaaaattt
    66721 gcgagaacca atgaataatc tggctttttt ctcctgtatc taattttttc cataataatt
    66781 gttcatgatg atataacgct aataacattg cgggcatttc taccatacgc tggtctcttt
    66841 catgtatttc tccccaataa tcgggatggg aaggatctgt tccagctttt attcctgata
    66901 gaattaattt caaccatcgt tcatctaatt ggtcaattgc gggccccgct ccccataaaa
    66961 cccgagagaa tccttccatt cctgcaattt tatttccata tcctacccct tgatgtatat
    67021 aatcaatttg tgcgccactt gctcgataaa acggctggag tggttgattt atctttttaa
    67081 ataagtcaaa gtaatcctgt ctggtcctta atagtggtac tggatgtttc attgtatttt
    67141 ctcctctttt tactaccaaa tagctaattc cgactgttct aatcgaagta agccttcaat
    67201 aaaaaaataa tcagaataga tcagtggcac attataagtc agttctctat ggtatgcttc
    67261 tgctccgcct ccaatgatcc catcttcatc catattccaa tttgcatagt gcttttctgt
    67321 cttttggagt atattgatag cagcttcatg atacattttt gatcgtacag aggaaacata
    67381 tcctgctaat tccagaagac cgcatgcagc gcatactcca gcactggtat caatattcga
    67441 atcttttaca gcaaaatcaa tgagaggaat ataaccagtt ttttcgattt tctgaataaa
    67501 atttactgct atcttttctg ctatagctag atagtacgga ttttttgtgt aacgataact
    67561 taacgcgaat ccatataaag cccatgcatt tcctctgctc caacaagaat tctcattgta
    67621 tccttgcccg ccaagtaacc gaatatattt ccctgattct gcatcaaatt ctgcgatatg
    67681 cccggttgat ccatctttcc tcactagata cttagctgtt gtatctgcat gctgccgagc
    67741 aattttagag aacctcggat caccagtttc cctcgaagcc caatacagta atggtaaatt
    67801 catcatacta tcaataatca tccaaccagg tttatggtca ttccatgcta ctaaatagtt
    67861 ccctgaaata ttatagcgtc cagcaagtac cgtagctgca tgtaacccta gttgtcgact
    67921 atgtttgttt tgagactgtt gataatgtgc aatagccgat ggtaagaata aaaaacctac
    67981 atcatgatga acgtctgaaa aatggtacag ggcagattct aataattgtt cttgttgtat
    68041 tgctgcctct ttgaactttt ctcgttttgt cttgtgaaag agctgccata aaataccact
    68101 ccaaaaacca ttggtccacc agtcgattcc tctgtccttt attacgtctg tatattttcc
    68161 gttgtaagaa atatatggaa ttttattccc cattctgtct aattccgcat ctatcttttc
    68221 agaagctaca ttcaaaattt ttcttgacca ttccttcgtc tgttcattca tggatatccc
    68281 gccattcttt taatttttat gaatgtggag ttgctcctag aacgaactag atggttaaca
    68341 ttcgttattt tttttactta tttgcagctc caatgtcatt ctccttaaat ttagactgcg
    68401 cccacacata aatcaagacc attgggatca ctgaagttgc taaaattagc ataattgcgc
    68461 cataattaat tggctcacct gtactagaaa ccgtcatatt tttgataatt aagggaaccg
    68521 tatatttatc tttatctgaa agcatgacta atggaaatag atattgattc caacttccca
    68581 taaagataag taaaccacta gtaataatcg taggtgataa aatcggtaca gcgattttcg
    68641 taaaaatcac ccactcattg gcaccatcga ttctggcagc ctccatcagt gctgtaggaa
    68701 attgactgct atattgtcgc atcataaaca ctgaagttgt actggcaatc gaagggatga
    68761 tcacacctgc ataagtattc gataatccta aacgactcat aatgtaaaat aaaggaatca
    68821 atgtggtaaa aaaaggcacc atcctagata gcataactgt attgaacaac agtttattcc
    68881 ccataaaacg atactttgct aatgcataac ccgccattgt agaactggct gtagctaaaa
    68941 ctgtactgat tacagcaata acaatcgaat tccaaataat caagggataa ttatatctcg
    69001 ccatgatggc ttgccagttt tttaatagca tatccccaaa cttgaaattg attccgccac
    69061 ttaaaatttc ttgattactt tgcgttgaag ctacaatcat atataaatat ggcatcaaaa
    69121 atattaaggc accaaacaca ataatgacgt acgtaaaaat tctgctttgt atcttttgtt
    69181 tagtcatttt tatcacctcc gaatttaaat tgaatagccg ataatataac agatacaaac
    69241 accatcgtcc aggcaatagc agaggctttt ccaaaatcaa ccatttcaaa acttgtttta
    69301 tataaattga ctcctacgac caaagctgaa ttttctggtc cattggaagt cccaaagaat
    69361 atatttggga tattgaaatt acccaagcca ttgatagtgg ctaaaactgt ggccattaaa
    69421 atgattggct ttagcatggg gatcgttaat aaaaagaatt gccgaaattt cgatgctcca
    69481 tctatttcag cactttcgta tatctcttta gaaatccctt gaagaccagc taaaaataaa
    69541 atagtgttat atcctacact aagccaaatc atcgtgatga tcattgatat tcgtatccaa
    69601 aaaggatttg ttaaccactg tacaccgtta attcctagcg cattcaaaaa tccattgaat
    69661 aacccagtcg gattgaaaaa agtcatgaat acggaagcta cagccaccgt tgatgtaatg
    69721 gttggaatat aatagacagt tctaaaaaat ccttttcctt ttaactgtgc tgaattcaat
    69781 aaaacagcaa agtaaatagc taatagcatc gttattggaa ttgatcctgc catgataatc
    69841 aaaacattcg tgtaagattt aataaacaat ggatctcgga agatagctag ataattgtca
    69901 aatccaacaa attcaaatga gtagccaatt ttcataaaac taaaagcaaa cgagataatg
    69961 atgggaatca aaatcgtcac aataaagaaa ataacaaaag gtgtgaccaa tccataagca
    70021 ctttttttat gagacgtatc tgttgctttt ttcttgctgt tctttattgt cttgcttggt
    70081 aacgaagatt gcatcatcgt taagtcctcc taatattttt taatcaccag ttaattttac
    70141 gccgtactta tgagcaaaat tttctgcttc ttttagatat gaattttcaa aatcacctgt
    70201 ttgccaatag ttatatgcag cggtagtcaa ataagacgta gcatctgaat aagtggctgg
    70261 taaaacagta tttgatgtat tttcattgat tttattcaat agttcatagt atttttgtcc
    70321 tccttagtag tcaaactttt tctcagctgc ttctgtttga taggccaatg agttagatgt
    70381 agccacgccc agctccagtg cgttggctaa gcattcttca tctgtcagca taaatgtaag
    70441 aaattgttgt gccacttgtg gatttttggc atctttaggg acataaaaac ttgatcctcc
    70501 tgatactgca ccttttcctg gattttcttt tgaaaaagga atcagtggag ccatgcgcca
    70561 tttgtcagat gcatcaggaa aattcaaaga catattcgta gcataccagc cgccagcaac
    70621 aaacattgca gattcttgaa atgctttctc agcatcttgt gcaccgtaga aatgtacaat
    70681 atctgagtcg atcatttctt tgattaactt tgcagcatgt ttcgcttctt tagtattcag
    70741 acggatattt ccttcttcat caagcaaaga ttgattctgc tgggctagca tgttgataaa
    70801 caattcttgg tccccagatt ctggcaaggc aattccaaaa ctaccagttt tttcttttaa
    70861 tgcttttgcc acctggatat attcttccca agaactgatt tcttcaaaat ctacacctat
    70921 ttcagcaaat ttttttggtt gataataaca aagtgctggg gtaaaatcac tagcccaagg
    70981 aataatcgtt tcattactcg tttgttgacg caaaatattc aatttttgat cataaaactg
    71041 atcgccatat ttgtcataaa atccatattc atcagcacta taaaaagcat cttgaaattt
    71101 ttccagcaca cctgctgctg aagtgtctgc catggagata atatccggca tatcttcgtg
    71161 tgttaccaat tttggggtga tcatttgtac tgttgctgtt cccgaagcta cattcacaat
    71221 ttttaagtca atatttgctc cagttttgcg gttatagatt tttgcagcat tttcaacata
    71281 gtttgtcagt ttgccgtttg cgacatatac ttctaatgta gtggcttttt ttatcattgg
    71341 atctgcgatg tttgctgtta cttcttcgtt agtttttcca catgcactta agctgaataa
    71401 tagggtaatg tttagcaaag ctatcaccaa tttgtatctt tttttcatgt ctatcccttc
    71461 tttcttttca agttcttata taaaacgctt tcacttatgt atattaacgg aattagtaac
    71521 acaaatattt atataaaaaa atattttact tgtcgttttg gcttttcatt tttgttataa
    71581 tagtcattaa ttataaatag aaagcttttt atataaaaag agaaaggaaa gtggttcttt
    71641 tgttaacaaa caaccaatcc tccaatcttg tcaaaaaaac actaacctat tccatagata
    71701 cccatcttca tttatctgct gctccctatt ttcgactact aataaatgaa agcgctaatt
    71761 tatatgctga atttgcacat aaatctatag aactagataa tccttccctt ttattcattg
    71821 ataaacagca agaagtccaa atcttttcta ctcaaaagga aacacaaact tttatcatta
    71881 tccagtttta ttttgatgag caattattag cacatattac gggacaatct ttttctatag
    71941 atcaagaaat aacatctatc attacttttc tgaaaaatga acagttcaaa attgattatc
    72001 taaaacatag taagccagct atttcctatg atcaggaaat caattctttt ttcatactat
    72061 gccaacagat gagcattcat ttgctttcct tattagttac ttattgtctg caaaaaattc
    72121 aattgtcttc cttaccaagg cattatcaac aggaacttgc tttattgaca ataccttcaa
    72181 atgagaaagt cattcctaaa aaaaagcata ttctttattc agatgaatta gttgatccaa
    72241 tcatacgata tttagaagaa catctttcag aaaatttttt gcttgaacaa ttagcccaaa
    72301 ttttttttat cagtccagcc agcttaaaaa aacattttag aaaagtgaat ggaaaaagtg
    72361 ttattagcta ttttaaagaa ctaaaaatgg aaaaagcaaa acgtctcgtg ctagaaaatg
    72421 aattaaccta tacagaaatt gcagatatat taggatttca ttcgatccat catttttctt
    72481 cagcttttaa aaaatataca gggttatccc cttcaaagta ttatctatcc ttaaaataga
    72541 aaaaaatttt taggttttat ccacaaatta tttctctata ttttctgtct attcaaaata
    72601 aacaataatt ttccggcaca attatttgct gttttttacc ataacatagt acgatcaacg
    72661 tagaaaagga gatgttttta tgaaagttgt tgtcattggc ggtgtagctg gcggtccttc
    72721 atttgccact cgtttccgtc gattgaatga agcgcacgaa atcattatct atgaacgcgg
    72781 agaaaatatt tcttatgcaa gttgtgcttt gccttattat ttaggaggtg tgatcactga
    72841 ccgtgactcg ctgatcgaac gtacaccaga aatattgaaa acaaaaaaca acatcgatgt
    72901 ctttactaaa cacgaagtaa cagcaatcga tccttctact aagcgattaa cagttaaaga
    72961 cctatccaca aatgaagaaa taaaaacaga ttacgataag ttgatcatct cttctggtgc
    73021 tagaccagat tatccggata ttcccggagt ttttgaagca gaaaacggct ttgtcctccg
    73081 tagtgtgacg gatgcggatc gaatcaaatc gttccttgaa gaaaaaaatc cacaacatgt
    73141 cgtcattctt ggtgcaggtg ttatgggtct ggaattagct gagaatttca agcatcgcgg
    73201 cttagacgtg actttaatcg atcaattgcc acaagtcgct ttcccttatg atccagaaat
    73261 tgctaattta gtttatgaca aattgctgaa agaaggatta gccgttcatt tagaaacaag
    73321 agttactgag atccgtgata aaggtcgaga aatagtatta tcagatggct ccgtcctttc
    73381 tgctgatatg ctaatttttg ctgttggtgt ttctccgaag aatgaagtgg tgaaagcaac
    73441 cggcatacaa ttatcagata caggtcagat cattgttgat gaccagttac aaaccaatct
    73501 gccggacatc tatgcgattg gcgatattat cgaaacgact agtgtagtga ctggtcagcc
    73561 gatccaaagt atgctttcca gtgcggccaa tcgtcaagga catatgctgg cagatatttt
    73621 aaatggtacg cctatgcgct atcgcggata tattggtgca ggtgtcgcaa aaatctttga
    73681 tcatacagca agttatgcgg gaatgacaga acatgaatta aaagcatcag gcataacaaa
    73741 ttataaaact gtttttatca ctccctttga ccatgcctat ttctatccag aagctacaag
    73801 attaaatcta aagctgattt ttgatgcaga tagcggtcgt attttaggtg gacaagcatt
    73861 tggagaaaaa ggtgtcgata aacggatggg agaactttct gtagcaatca ccggaaactt
    73921 gacagtcttt gatttgcctg atttggagtt gccttactct ccaccgtatt ctactacccg
    73981 tgacccgttg aatatagctg gttatgtcgc aatcaatcaa atgacgaata tcgtagaaac
    74041 gatcaaagca agtgatatac ccgaaaacga tttgaaagaa gcgttctttt tagatatacg
    74101 tgaacctaat aaagcacctt ctggcagtat ctcggcaaca aaaaatattc caatgaatga
    74161 gttacgagat cgaatcaatg aaatcccaaa agataaaaaa gtttacatta ctttcagaag
    74221 aggattgaat acttatactt ctgcccgaat cttggcaggt ttgggtatca aagcgatttt
    74281 gattgaagaa taaaaaaacg accgcggtgg gtagccgcgg tctaggagtt atttttactt
    74341 gaggagtaaa aatgaaaaag tatatagggt tgttggttgg tatgtatata gaatagaaga
    74401 taaatgtgaa gaaattgtgc attatttctt cacatttatt aggttatagt ttaagaaagt
    74461 ttaaacattg cgcaatttaa ttattttagc tgtttctttt cataaaaaaa catgttgtga
    74521 ctctgagaca aatcatagtc catagtgatg tctctagagt aactacgcct aatattacga
    74581 ggcatcatat taatctatca gcactagtac ctaaactagc atccctttcg aatatttttt
    74641 cttatataat aaagtttgct ttcactcctc taatctcacc aaatcgactg gcttgcgttg
    74701 tgtgatacta ggaactgatt ggtttagaac gacttttcta ttgtaatcat atttttcgcc
    74761 atcctcgttg tcttctaatc cttcaatgaa tgatccctaa tgtgtgtatt aaataattta
    74821 atttttttgt tcctatttat ttaa
//
LOCUS       DACDXO010000006        66048 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E23605_2017
            SAMN12639989-rid9881463.denovo.006, whole genome shotgun sequence.
ACCESSION   DACDXO010000006 DACDXO010000000
VERSION     DACDXO010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639989
            Sequence Read Archive: SRR10023389
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 66048)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 66048)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741914.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:42:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,972
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 33 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 17 of 73
            Pseudo Genes (multiple problems)  :: 10 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..66048
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639989-rid9881463.denovo.006"
                     /isolate="E23605_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(27..725)
                     /locus_tag="IWM35_000512"
     CDS             complement(27..725)
                     /locus_tag="IWM35_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobyric acid synthase"
                     /protein_id="HAP9918390.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(718..2070)
                     /locus_tag="IWM35_000513"
     CDS             complement(718..2070)
                     /locus_tag="IWM35_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HAP9918391.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            complement(2293..3693)
                     /locus_tag="IWM35_000514"
     CDS             complement(2293..3693)
                     /locus_tag="IWM35_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HAP9918392.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            3854..4591
                     /locus_tag="IWM35_000515"
     CDS             3854..4591
                     /locus_tag="IWM35_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HAP9918393.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            4716..5195
                     /locus_tag="IWM35_000516"
     CDS             4716..5195
                     /locus_tag="IWM35_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HAP9918394.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            5192..5959
                     /locus_tag="IWM35_000517"
     CDS             5192..5959
                     /locus_tag="IWM35_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9918395.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            6189..6758
                     /gene="lepB"
                     /locus_tag="IWM35_000518"
     CDS             6189..6758
                     /gene="lepB"
                     /locus_tag="IWM35_000518"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAP9918396.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(6862..7464)
                     /locus_tag="IWM35_000519"
     CDS             complement(6862..7464)
                     /locus_tag="IWM35_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAP9918397.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            7570..8039
                     /locus_tag="IWM35_000520"
                     /pseudo
     CDS             7570..8039
                     /locus_tag="IWM35_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325408.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            8145..8729
                     /locus_tag="IWM35_000521"
     CDS             8145..8729
                     /locus_tag="IWM35_000521"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HAP9918398.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            8732..9805
                     /gene="prfA"
                     /locus_tag="IWM35_000522"
     CDS             8732..9805
                     /gene="prfA"
                     /locus_tag="IWM35_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HAP9918399.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            9798..10637
                     /gene="prmC"
                     /locus_tag="IWM35_000523"
     CDS             9798..10637
                     /gene="prmC"
                     /locus_tag="IWM35_000523"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HAP9918400.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            10630..10809
                     /locus_tag="IWM35_000524"
     CDS             10630..10809
                     /locus_tag="IWM35_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9918401.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            10831..11856
                     /locus_tag="IWM35_000525"
     CDS             10831..11856
                     /locus_tag="IWM35_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HAP9918402.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            12031..13275
                     /locus_tag="IWM35_000526"
     CDS             12031..13275
                     /locus_tag="IWM35_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HAP9918403.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            13428..14375
                     /locus_tag="IWM35_000527"
     CDS             13428..14375
                     /locus_tag="IWM35_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP9918404.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            14509..15138
                     /gene="upp"
                     /locus_tag="IWM35_000528"
     CDS             14509..15138
                     /gene="upp"
                     /locus_tag="IWM35_000528"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HAP9918405.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(15277..15519)
                     /locus_tag="IWM35_000529"
     CDS             complement(15277..15519)
                     /locus_tag="IWM35_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9918406.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            15640..15712
                     /locus_tag="IWM35_000530"
     tRNA            15640..15712
                     /locus_tag="IWM35_000530"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15673..15675,aa:Thr,seq:cgt)
     gene            complement(15831..16469)
                     /locus_tag="IWM35_000531"
     CDS             complement(15831..16469)
                     /locus_tag="IWM35_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAP9918407.1"
                     /translation="MNNILGKKIELKKGILGTIVVVLGLLGLLFAPSIISIFSLTITG
                     FIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPI
                     NEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNG
                     NILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
     gene            complement(16592..17224)
                     /locus_tag="IWM35_000532"
     CDS             complement(16592..17224)
                     /locus_tag="IWM35_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HAP9918408.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADFVDCSLTKIMFSTNDLENA
                     SFFDTDLKNIDLSSNSFEMIEVRSEHLKNCTLNQLQALGFARKFLQIKVV"
     gene            17575..17874
                     /locus_tag="IWM35_000533"
     CDS             17575..17874
                     /locus_tag="IWM35_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HAP9918409.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
     gene            18369..20534
                     /gene="acm"
                     /locus_tag="IWM35_000534"
     CDS             18369..20534
                     /gene="acm"
                     /locus_tag="IWM35_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HAP9918410.1"
                     /translation="MKKCRNIILSMLFIITNITSLIPVHVYADAGRDISSNVTSLTVD
                     PTNITDGGNIKVKFSFDEKKQNIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVG
                     TLTVRKDSAQVVFNENIKNLDSVEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATV
                     NVTKPASGSSSSVFYYKTGDMLPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRL
                     DPSTFEINQIHLGEQKVYRGEEGIQQFLQDFPSATFNFSVTDNYIEITIPKNFVNLRK
                     IMVSYKTIIENPEQINFENHSEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGE
                     LKIFKYINDTEIGIPNVTFELRRADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVK
                     EKEAPNWIDFDPLSSNELKFSINENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPS
                     IYFKLFRASTNNWEPVPDAETKRLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGN
                     DYVPSGYQKIENGLVVTNENKEVISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQ
                     HKEVSEKDDWRYRFTNLPKYRDGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNI
                     SIPGSHITPWKNFPEKTEREKIRNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITN
                     KSKSILPKTSSKKSSFAVIIGLLIVTICAGIFLQKYKKV"
     gene            complement(20806..21291)
                     /locus_tag="IWM35_000535"
     CDS             complement(20806..21291)
                     /locus_tag="IWM35_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HAP9918411.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     gene            21771..22412
                     /locus_tag="IWM35_000536"
     CDS             21771..22412
                     /locus_tag="IWM35_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAP9918412.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIIMKQVEGKLNYYR"
     gene            22431..23066
                     /locus_tag="IWM35_000537"
     CDS             22431..23066
                     /locus_tag="IWM35_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAP9918413.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            23084..23899
                     /locus_tag="IWM35_000538"
     CDS             23084..23899
                     /locus_tag="IWM35_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP9918414.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
     gene            24218..25654
                     /locus_tag="IWM35_000539"
     CDS             24218..25654
                     /locus_tag="IWM35_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP9918415.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            25843..28290
                     /locus_tag="IWM35_000540"
     CDS             25843..28290
                     /locus_tag="IWM35_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HAP9918416.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            28520..29587
                     /gene="rlmN"
                     /locus_tag="IWM35_000541"
     CDS             28520..29587
                     /gene="rlmN"
                     /locus_tag="IWM35_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HAP9918417.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(29780..30883)
                     /locus_tag="IWM35_000542"
     CDS             complement(29780..30883)
                     /locus_tag="IWM35_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAP9918418.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            31436..31984
                     /gene="aac(6')"
                     /locus_tag="IWM35_000543"
     CDS             31436..31984
                     /gene="aac(6')"
                     /locus_tag="IWM35_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HAP9918419.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
     gene            32530..34191
                     /locus_tag="IWM35_000544"
     CDS             32530..34191
                     /locus_tag="IWM35_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP9918420.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
     gene            complement(34261..34608)
                     /locus_tag="IWM35_000545"
     CDS             complement(34261..34608)
                     /locus_tag="IWM35_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HAP9918421.1"
                     /translation="MKKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFLWSLPF
                     LIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSSLSSASYGMYSFWLIIAGILI
                     ALSAIFMLFSLLG"
     gene            34692..35633
                     /locus_tag="IWM35_000546"
     CDS             34692..35633
                     /locus_tag="IWM35_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9918422.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            35634..36677
                     /locus_tag="IWM35_000547"
     CDS             35634..36677
                     /locus_tag="IWM35_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9918423.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            36683..37753
                     /locus_tag="IWM35_000548"
     CDS             36683..37753
                     /locus_tag="IWM35_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9918424.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            37743..38696
                     /locus_tag="IWM35_000549"
     CDS             37743..38696
                     /locus_tag="IWM35_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP9918425.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            38708..39718
                     /locus_tag="IWM35_000550"
     CDS             38708..39718
                     /locus_tag="IWM35_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP9918426.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(40003..40824)
                     /locus_tag="IWM35_000551"
     CDS             complement(40003..40824)
                     /locus_tag="IWM35_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HAP9918427.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(40921..41199)
                     /locus_tag="IWM35_000552"
     CDS             complement(40921..41199)
                     /locus_tag="IWM35_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9918428.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            41467..43476
                     /gene="metG"
                     /locus_tag="IWM35_000553"
     CDS             41467..43476
                     /gene="metG"
                     /locus_tag="IWM35_000553"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HAP9918429.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            complement(43955..44542)
                     /locus_tag="IWM35_000554"
     CDS             complement(43955..44542)
                     /locus_tag="IWM35_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9918430.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            44717..46039
                     /locus_tag="IWM35_000555"
     CDS             44717..46039
                     /locus_tag="IWM35_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP9918431.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            46194..46970
                     /locus_tag="IWM35_000556"
     CDS             46194..46970
                     /locus_tag="IWM35_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HAP9918432.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            46963..47532
                     /gene="rnmV"
                     /locus_tag="IWM35_000557"
     CDS             46963..47532
                     /gene="rnmV"
                     /locus_tag="IWM35_000557"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HAP9918433.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            47525..48409
                     /gene="rsmA"
                     /locus_tag="IWM35_000558"
     CDS             47525..48409
                     /gene="rsmA"
                     /locus_tag="IWM35_000558"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HAP9918434.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            48860..49489
                     /locus_tag="IWM35_000559"
     CDS             48860..49489
                     /locus_tag="IWM35_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9918435.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(49613..50722)
                     /gene="ugpC"
                     /locus_tag="IWM35_000560"
     CDS             complement(49613..50722)
                     /gene="ugpC"
                     /locus_tag="IWM35_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HAP9918436.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
     gene            50931..51353
                     /gene="mgsA"
                     /locus_tag="IWM35_000561"
     CDS             50931..51353
                     /gene="mgsA"
                     /locus_tag="IWM35_000561"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HAP9918437.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     gene            51676..52212
                     /locus_tag="IWM35_000562"
     CDS             51676..52212
                     /locus_tag="IWM35_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HAP9918438.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
     gene            complement(52252..54015)
                     /locus_tag="IWM35_000563"
     CDS             complement(52252..54015)
                     /locus_tag="IWM35_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9918439.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
     gene            complement(54018..55733)
                     /locus_tag="IWM35_000564"
     CDS             complement(54018..55733)
                     /locus_tag="IWM35_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9918440.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
     gene            complement(56512..56736)
                     /locus_tag="IWM35_000565"
     CDS             complement(56512..56736)
                     /locus_tag="IWM35_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9918441.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
     gene            56936..59704
                     /locus_tag="IWM35_000566"
     CDS             56936..59704
                     /locus_tag="IWM35_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP9918442.1"
                     /translation="MLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNTVSH
                     YLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQPKDL
                     FSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLHDLL
                     EDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLDEVH
                     RLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRIPIR
                     IELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGELKNI
                     VKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLMEKQ
                     QPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHELLVY
                     WTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELEQQV
                     SQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIAAHG
                     YATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILVDMG
                     SLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPEWRL
                     LYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNSDPL
                     FSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQRII
                     RNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLIERL
                     IRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSEDTE
                     FLSIEEEF"
     gene            59785..60204
                     /locus_tag="IWM35_000567"
     CDS             59785..60204
                     /locus_tag="IWM35_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HAP9918443.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            60216..60686
                     /locus_tag="IWM35_000568"
     CDS             60216..60686
                     /locus_tag="IWM35_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP9918444.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            60703..61482
                     /locus_tag="IWM35_000569"
     CDS             60703..61482
                     /locus_tag="IWM35_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP9918445.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            61469..62290
                     /locus_tag="IWM35_000570"
     CDS             61469..62290
                     /locus_tag="IWM35_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP9918446.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            62307..63356
                     /locus_tag="IWM35_000571"
     CDS             62307..63356
                     /locus_tag="IWM35_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP9918447.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            63369..64373
                     /locus_tag="IWM35_000572"
     CDS             63369..64373
                     /locus_tag="IWM35_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP9918448.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            65006..66025
                     /locus_tag="IWM35_000573"
     CDS             65006..66025
                     /locus_tag="IWM35_000573"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HAP9918449.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
BASE COUNT        22102 a        11476 c        13457 g        19013 t
ORIGIN      
        1 ttgtcacatt cccctttttc gaataactag gctgtttgtc cttcaagata gggcagatcc
       61 aattctttac cgtaacgttg ttctaaagct aggcggatca aacgagctgc caggttctca
      121 tttcttgcaa ggataggtcc atggaaataa gaaccgaaga catttcggta aatgacacct
      181 tcagattggt cttcaccatt atttccttga ccttgtacca ctttccccaa aggtcgttca
      241 ccttcaccta agaaagtccg accattgtgg ttttcaaatc cataataagt ttcattgaat
      301 tcttcgttat ggataacgat atcaccgata aaccggttat tttcctgact taacgtgtag
      361 tgatccagtg cactgattcc ttggatcttt tctccattag ctcccatgta gtagtgacct
      421 agcaattggt agccaccaca aatggctaac atgacgccat cattttcgat atagttgatc
      481 aaagaatctt tctttttctg gatgtctttt gaaatgatta attgctcaaa gtcttgacca
      541 ccgccaaaga aaaccaagtc atatttatca gcatcaaaag gttcatgtat actaacgatc
      601 tccgtatggc aagaaacccc cattttttct gctatatatt tcaacatcaa aaggtttccg
      661 ttatcaccat atgtgttcat caaatttcca taaaggtggg cgatattgag ttcataatta
      721 gccacggttc attcctcctt tgataaatcc ttgagcagct agttctttac gaagctgcaa
      781 tacagcagtg taagtagcta aaatatagac atgctcagtt ggcaattctt tgattttttc
      841 gattacttct ggtaattctt ttgtttctgt caatttattt tctggaatcc cggcaacttt
      901 tagacgtaaa gtcatatctg tatgacggtc tcctccagaa ataacagctg ggatatccat
      961 atcggcaaag ttttcaaaat taccatccca aatccagctg acatcgattc cgtctgcgta
     1021 attggcattt aataaagcca ctaaagaaaa tggataagga gctagaccaa tcatatcgat
     1081 tacttgattg atacctacag gatttttgac aagtactaat gtacattttt tcccattgat
     1141 attgatgact tcttgtcggc cgaatacttt ctcgtcatat cctaaacctt gtttgatttt
     1201 atcaggatcg acattgaagt actctgccac tgcagtagct gctaaagcat tgtagacatt
     1261 atacataccg ccaacttcga ttccgtattc atgaccatcg atcacgaaat ctgctgacac
     1321 attgtccatc cgtaccatat cagttaatcg ataatccaat tcaggacgtt tgaagtcaca
     1381 atttggacag taatatttcc ctaagtttgc ataagtgatc attttataat gcaagatgtg
     1441 atgacaattt ggacataaga caccgtctgt attgtaatga gccatttgtt cacgatcttc
     1501 ttcatggtca aagccataat attttctagg attgactgtt tcgatagaat tgaagattgg
     1561 tgaatcaccg ttactgataa tcgttgcatt tggtgacttt gcggcaccgt ctacaatcat
     1621 tttataggtc gtatagattt ctccataccg atccatttga tcacggaaaa tatttgtaaa
     1681 taagaacagt tctggtttca tatattctgt gactttgctc aaacttgctt catctatttc
     1741 taggacagca aactttttct gtcctttttt tggttttgcc tgcaagaaag tcgatacaat
     1801 tccctgtacc atattcgcac cagtagggtt ggttagaact tcatcgaatt gctggcgcaa
     1861 aatattgact gttaaagcgg tagtcaatgt tttaccgttt gttccggtga caacgacgac
     1921 ttggtagtct tttgccaaac tatctagtat atgtggatcg actttcagcg ctagtttacc
     1981 tggtaaactt gatccgcctt taaaaaaagt ttttaatgcc cattgcgacg tttttccaac
     2041 tgcaatcgca aaatgactgc gtattcccat tatgtaacct ccaaaaacga tttgctgacg
     2101 aataaattca actaatcata ccatatgttg aaaaaaaggt taaattgctt ttcttattct
     2161 taagaaaagg ttacggctga aagtgcatga aatcggacaa aatcgcatga tttaacaaaa
     2221 aaacagcttg aaacaaaatg attgtttcaa gctgctgtga ttttattatt ccctcttacc
     2281 tatattccca ttttaaccga taataatttt ctcgcttgga tattcatagc ctagatctcg
     2341 tgtttctttt ggcacgaata acatcaatgt atatagcatt ccgatccgcc caataaacat
     2401 cagtgtagca atcgtgattt tgcccacaac agacaagtct ccggtaatcc ctaaagataa
     2461 accagttgtt ccaaacgcag aagttacttc gatgatgatc gagatcagcg tttgttcttc
     2521 tgtagcagat aaaaaaacga tacaaaagaa acacatacca agagaaagca taaagacaac
     2581 gactgatttt cgcacatcat cttgatcgat tctccgtcca aaaatattga tgttatcttc
     2641 acttttgagg aaggaatata gatacaaacc gatgattgct acagtagttg ttcgaatccc
     2701 gccgcctaca gaactaggac tgcatccgat aaacatcagt aaagaaaaaa tgatcagtgt
     2761 tgtgatttga aaatcaccca aatcgtgaat ctgcagacca gcattacgag tcgtaatcga
     2821 ataaaacatc gaattgatcc atttgacact catattggaa tcttggaata aatggtcctt
     2881 ttccaataga taaatcaaga cagttcccga aacaaacaag ataacaaaag ctaacacagc
     2941 taatttggta aacaaggaaa aacgaaacgg aagcttcgca ttggaacgtt tgtataatag
     3001 ccattcccgg cactccatca atacaggaaa accaattcct ccgacaaaaa tcaaaaacat
     3061 gatcaggatc aagaaaaaat agtcatgtgc aaagggcttg atggaatccc cagtgacgtc
     3121 aaacccagag ttcgtgacag ccgatatcgc ctgataaaaa ccgtaaaaaa cggcatcacg
     3181 ccaacgatca aaatagcctc tgtaataaaa ataaatagaa aagaatgtcc caaaaagtaa
     3241 ttgaaaccag attaaaatag caaaagtgat ccgaatcagt cggacgatac cagacaaacg
     3301 cggttgattc atatccgtca tgatcagctg tcgctgtttc agtgtgatcc gtcgtttaga
     3361 aaaaatgaca aaagcagtag aaatcatcat gatccccaaa ccaccaactt gaaataatat
     3421 ttccagcagt ataataccgt tatcattgaa cacagaattg atatcaaaag tcgatagtcc
     3481 agtcacactc actgtactga ttgccatgaa cagcatgtcc acaaaagaaa catggctatc
     3541 tggttcgcga aaaatcggca tataaaatag aacgagtgat aagaccgtca tcaatatata
     3601 ataaaaaacg atgatttgga tcgatgagaa atgattggca gcaaacttct gtcctcgccg
     3661 ctgcagcctt cgaaagaata aatcaacatg cacagtacta tcctcctgac aatagtcgtt
     3721 tctaccagta tacaagggga attaggaata ctcaaagaaa ttatcaaaca gataacaata
     3781 cagctttcga ttaaaataga aatggcttat aataaggaaa atcctagaaa aaagaaggag
     3841 gaggaataac aaattgatta atgctcaaca aatcgtagaa aaaatgaaaa atcaaaaaat
     3901 aaattatgat acagttctga aaaaaatgat catgcaatgg gagaaagaag aagtaaagcc
     3961 taagatatta atacatacat gctgtgctcc ttgcagtacg cacacattgg aattcatgtc
     4021 tcaatatgca gagatcacgt tatttttttc aaattcgaat attcatccta aaagtgagta
     4081 tttgcgaaga ttacatgaac aaaaacgatt tgtagaccaa tttaatgaga aaactgacca
     4141 tcatgttgcg ctaatcgttg atgaataccg tccaaatgaa ttcaccaaaa tggttttaac
     4201 aaaccatctt gaaaacgaaa aagaaggtgg tgctcgctgc agtgcatgct tcaatatgcg
     4261 attagatctt gttgcgcaaa aagcgcaaga actaggatac gattactttg gaagtgccct
     4321 gaccatctcg ccaaagaaaa atgcagcttt gatcaatcag atcggaatgg atatccaaaa
     4381 gatttacggg acacaatact tgccaagtga tttcaagaaa aacaaaggct atgaacgctc
     4441 tttgcaaatg tgcaaagagt atgatattta tcgccaatgt tattgtggtt gcgtgttcgc
     4501 tgccaagcag caaggatgcg atctgaaaga aatcaatcgg gaagcaaaag cttacttaaa
     4561 aacatcaacc gtagtttttg aggaaaatta aagaaatctt tcggaaaaac gtaagaaaaa
     4621 aataaagaat ttatggtaga aataaggtag actgtataat ttgtccgtag taaagtaagg
     4681 aaacaagaat aaatttcaat gaaggaagag ataagatgcg aaaagtagca cctattttga
     4741 agccaatcgc tgtattaaca atctcgacga ttgtcgcgac tactacaggt attgtgaata
     4801 gttccagcta ccgtgcacca agttcaagtt ctagtactgt agaagaaaca acaccaacta
     4861 cttcagtctc gacatctgaa ccaacaacaa cggaaagttt gaatgaaacc actacgacaa
     4921 gtacatggga cgatacaaca actagttcgt cgtatgatga gacatataca actgacagct
     4981 caagcgaaac aagtaatacg gtaccagatg gcactccatc ggattcaacc aatccaagta
     5041 caagtccgga tacaagtgct agctccgaaa cgataccaag cagtacgact gtagatgata
     5101 acacgcaaaa tgatgatgca gcacgtacag cacagcaaaa ccaagattca ggaaataccc
     5161 aaacgtataa ccagcaagca ggtgattctc aatgacattt ttactagacc ttatcaacgg
     5221 ggtgcataaa ttccttggct atttagacat cagtcctaaa tatcttaacc gtggatatac
     5281 gattttaagt gtgattccca cgctttattt attgagaatt gtttatggac tttggcaaaa
     5341 ccaaaactat cttcaatttt tcttatatgg acttgcattt ctagtgcttg tttattttac
     5401 tgttttaaat gtattttatt actttttaga taaaaacacg aaagccgatg tcactcagct
     5461 ttttgtgaag tatttacctg atgaagcgtt caacatccag acagaagaat cgatagataa
     5521 tggaaattcg atcgataaag tgaatacaca agaggtcatg gtttcttatg acgaagatta
     5581 tcaattgaaa ttagccgaga atatgcggta tttgattgga aatggtgaga tcaaaacaaa
     5641 tgaactcggt cgcatggatg gatttctggt cgatagaaat acattgtatc cttattacta
     5701 tgtcaagaaa acaggagaga aagagtatgt tctaaatatt ggacgaagct actcggacat
     5761 ggaaaaagta gggaccattc gacttgactc aacagaagac acattggaac ctgtcggatt
     5821 gttcatcgtc ggcggtgatt ttgtcaaaga cgggttacgc tatcatgagc cttatcgtct
     5881 gaaattgatt gcaaaaaagg cacagtcgca ggaagaacca gtggtttata gtcgtagcca
     5941 aagaagaaga cattcataat gtgatgaaac gccgtgtatc agtggttgcg ataaaaacgt
     6001 ttattcggaa taagggggat gtgacacgtc ttttgtcacg ttcctttttt atataacgag
     6061 aaagataaga cgaacacatc acttattctt tcctttcatt tataaacatt gtagaataaa
     6121 gaaagtacaa cgaagtattg tgaaaaaacg taataaataa cgaggaaaat acaagtagga
     6181 ggtaagctgt gaaagaattt ttgaaaaatt gggggatttt gatccttgta ttagcggcta
     6241 ttttattagc tagagtgtac gtgtttacac cagttactgt aaacgggcat tcgatggacc
     6301 ctacattaag tgatggacaa cgattgattt catctaagat ctcaaactat gaacgaatgg
     6361 atattattac tacaaaggaa ccgggggatg aagaacggat gattgttaaa cggatcatcg
     6421 gtatgcctgg agatacagtc aagatggaaa atgaccagtt gaccattaat ggcaaaaagt
     6481 atgatgaacc ttatctggat gagttcaaaa aagaattttc agaagataag ttacaaggag
     6541 aatatgctta tagctcagga ttccaagcac aagcagaaag ttcatccacc tttacgagtg
     6601 actttgaata tactgtgcca aaaggaaagt atcttgttct aggcgataac cgcttgattt
     6661 caaaagacag ccgaatgttt gggttagtcg acaaagatat gatccaagga aaagttgttt
     6721 tccgttattg gcctctttca gaaattaaaa tcatgtgatg aaaaaataaa aagaagctgt
     6781 gacatgtttt ttgtcacagc ttctttttcc gaaaattcct tcttatttct tggagttgct
     6841 cacttttttt gcaatctcat tttaagcttt ttggctgatg gataagaaaa gcgaagcgtt
     6901 tttgttcata aatagctttt ccccacgatg gaaggtatga ctagtagcag agacaaagcc
     6961 cgttttttcc aatcgattat tcaattcttc ttggctaaag ccattatgga ctttcggatg
     7021 actaatttgt tcattcttgt caaagtctac gatgatcaat tgaccgccag gagctaaaat
     7081 ttcataaagt tttgtaagaa tattctccgt atcaggaatg tgtaaaagta caagtgatag
     7141 aaggatgaga ttagcttgga cagcaggaaa ttcgacagaa gcatcagcgt ggatcgttcg
     7201 gacatttttc agatctgctg cttggatctt ttcttctgcc attttcagca tagtttctga
     7261 agcatcggca atgatcagtt ctttgaatct ttccgcaagc ggtaaactaa caagcccagt
     7321 tccgcctccg taatccagca ggactttttg gtggctgtcg cgcggtagat aagtagttag
     7381 ttcttcgcga ataatcttgg ctaatgcttg acggtcaggg gaatcataat ggttagcgat
     7441 ggaatcaaaa atattttttg tcatcaaata gctccttacg taaaactttg aaattttaac
     7501 ttattatagc acgtgaaaag aaagaaagca tgctatactc aattcaaaaa gggagaatct
     7561 agaaagaata tgccgatatc gtttgtaaaa gatagagagg aaaaaggaaa gtgtgtccga
     7621 gaaatattac tagacttgcc tgagtggttt gggcttcctg aatcaacgga aaaatatatc
     7681 gaggaatcaa gtaaactacc tttatggtgt gaaaaaagaa aagaagaata tttaggcttt
     7741 atcactcttt cacagacaag tgaagatact gccgaaatct attctattgt atgggaataa
     7801 aaaaagacta tcatcaccaa ggattaggaa caaaactttt taaagaagca gaaggttacg
     7861 cagcaaaaca ttacaaatat ttgcaagtga agactgtaga cgaggggcac tactctatat
     7921 atgatcaaac tatttgtttc tacgaatcat taggattttc aaggttggaa gtttttccaa
     7981 atttatggga tgaatggaac ccatgtttag ttcttgtcaa aaaattggaa caaaagtgag
     8041 caatagattt aatactaatc tgtgcttgat gaaaattctc actgtaaaaa atgctgaaaa
     8101 tcaagtataa taccatagga acaaactgga aggtgaaaaa gagcatggca caactatttt
     8161 ttaagtatgg cgcaatgaat agcggcaaga cgatcgagat cctaaaagtc gcacataatt
     8221 atgaagaaca aaataagcca gttgtcttaa tgacaagcgg tattgataca agagatggtt
     8281 taggcgttgt ttcaagccga atcggactga aacgtaatgc gatgccgata tttgaagaaa
     8341 cagacgtatt tgacgtaatt cagaaaatgg aaagcaaacc ctattgtgtg ctgatcgatg
     8401 aagcgcaatt cttaaaaaag aaacatgtca ttgcttttac gagaattgtg gacgaattgg
     8461 atattccggt tatggcattt ggtttgaaaa atgattttcg aaacgagtta tttgaaggat
     8521 caaaatattt attgctttat gcagacaaaa tcgaagaaat gaaaacaatc tgttggttct
     8581 gccacaaaaa agcaatcatg aatttacatt atattgatgg aaaccctgtt tatgaaggcg
     8641 atcaagtgca aattggcgga aatgaagctt attaccccgt ttgtcgaaaa cattactttc
     8701 acccatcaat gattacagaa ggagaataag catgtacgat caattacaag caattgaaga
     8761 ccgttatgaa gagcttggag aacttttaag tgacccagaa gtgatttctg atacaaagcg
     8821 ttttatgcag ctttcaaaag aagaagcaaa cacgcgcgaa acagtcgaag tttatcgtcg
     8881 ttataaagaa gtagtacaag gaatcaccga tacagaagaa ctgcttggtg aaaaactgga
     8941 tgatgaaatg gctgaactag caaaagaaga attatctgaa ttaaaaaaag aaaaagaagt
     9001 cttagaagaa aaaatcaaga tcttgctttt accaaaagat ccaaatgacg ataaaaatat
     9061 catcatggaa atccgtggag cagcaggcgg agacgaagca gcattattcg ctggcgatct
     9121 tttcaatatg taccaaaagt atgctgaagc acaaggctgg aaaacagaag tgatggaagc
     9181 taacgttacc ggaatcggtg gatataaaga agtgatcatg atgatcaccg gagacaacgt
     9241 atattcgaaa ttaaaatatg aaagtggtgc gcaccgcgtc cagcgtgttc cttcaactga
     9301 atcgcaaggg cgtatccata cttccaccgc aacagttgtc gtgatgccag aagctgaaga
     9361 agtagagatc gatattgaag acaaagatat ccgtacagat atctatcatg cttcaggtgc
     9421 tggcggacag cacgtcaata aaacagcatc tgctgtacgt ttgactcact taccaacagg
     9481 gatcgttgtt gctatgcaag atgaacgttc tcaaatcaag aaccgtgaaa aagcaatgaa
     9541 aatcttgcgt gctcgtgtgt acgataagat ccagcaagaa gcacaaagcg agtatgatgc
     9601 caatcgaaaa tcagcagtcg gaactgggga tcgttcagaa cggatccgga cgtataattt
     9661 cccgcaaaat cgtgtgactg atcatcgtat cggattaact atccaaaaat tagatcagat
     9721 tttagcaggg aaattagatg agatcgttga tgcattgatc atgtatgatc aaacatcaaa
     9781 actggaagct atgcaaaatg ggtaaaatga cctatcgaga agtcctgtca cgggcttctt
     9841 cttttttaga ggatcatggg aaagaagcat atagtatcca atttttattt ttggaacgaa
     9901 aacaatggaa aaaacttgat tggctccttc atatgaacga agagatttca gaagaagaac
     9961 agcggttgat cgaaaccgat ctgcgacttc tgcttgcaga tcatccacct cagtatttgc
    10021 taggatatgc agatttttat gatcatcggt taaaggtaac tgaagcaaca ctgatccctc
    10081 gaccagaaac agaggagtta gtcgaatggt gtttagacga gacgcctggt gtaccgttag
    10141 aagtgataga tattgggaca gggactggag caattgctat tagtttgaaa gcagcaagaa
    10201 aaaattggca tatatctgct gtggatcttt cagaagaagc attagaagta gcgaaagaaa
    10261 atgctcagaa agaaggaacg aaaatcagtt tttatcatgg agatacatta ggaccagtaa
    10321 tggatcagca atttgatgtg attatttcta atccgccgta catcagtcgg aatgaatggg
    10381 aactgatgga tgaaagtgtc cggtcattcg aacctaaaat ggcccttttt gctgagaacg
    10441 atggattagc tatttatgaa aaaatagcag ttgaagcacc aagcgtacta aaatcaaatg
    10501 gaaagatttt tttagaaatc ggatttcgcc aaggtgaagc agtaaaaaaa atatttcagc
    10561 aagcatttcc agataaaaaa gtggcgataa aaaaggatct ttttggcaat gaacgaatga
    10621 tccaagttta tggttgaagt tgtggatgaa aaatttttta tttgtttttt tattataaaa
    10681 aaacacttat ttgacaagga tgtaaacgac tttttggttt atcaacaagt ttatccacta
    10741 ttgacaaata gattatccac aaacagtgga aaacttgagg ataaactcag acaaaataag
    10801 aatgtatgaa aaaggggttg tggataaact gtggaaacga aacgatatga tgaaacagaa
    10861 ttgcaagagg cagccgaagc actgaaagaa ggagaacttg ttgcttttcc taccgaaaca
    10921 gtatacggat tgggcgccaa cgctctttta ccaaacgctg tcaagaaagt gttttctgta
    10981 aaagggagac cgcaagataa tccattaatc gtccatgtcg cctcttttga acaagtaaaa
    11041 gagtatgtgg acaactttca tccggcagca gagaagattg tgaagaactt ctggcctgga
    11101 ccattaactt tgatatttaa gatcaaaaaa gacactttgc cctcagttgt gacgggagga
    11161 ttatccacag ctgcatttcg tataccggat aacaaaaaaa cactagaagt aatcgaactt
    11221 tccggtgttc cgcttgtcgg tccaagtgca aatacgtcgg gaaaaccaag tccaacgact
    11281 gcagatcatg tatatcatga tctacaagga aaaattaccg ggattattga tgatggagct
    11341 acgcgaattg gtgtagaatc cactgttctt gatttaagtg atccaacagc catgcctatg
    11401 attttacgac caggtgcagt aacgaaagaa caaatcgaag cagtcatcga atctcctgtt
    11461 gctattgatc agcatcttgt aaaagaaaat gaaacaccaa aagcccctgg gatgaaatac
    11521 aaacactact cacccgatac acgtgttctg atggtcagag atggcgattg gtcaactgca
    11581 gttcaatggg caaaaaataa gaagatccgt gcaggtgtca tagcaagccc cgaaattgcg
    11641 gatcaagtgc ggacagatac tgcagctgtt tatatgtata acgacaactc agtagaagcg
    11701 gcggctaaag gattgtttgc aggtctaaga ggattagatg aaccaacact tggattagat
    11761 ttgatttttg tacaagtgta tccagaaact ggattgggga atgcttacat gaatcgcttg
    11821 aaaaaagcag caggacaaaa ttattttgaa aaatgatcaa ttggttattg tcgcattctt
    11881 ttagtatgat acaatgctat caagtaaatc ttctatcagt gaacgaaaaa ctaagcgtaa
    11941 ctaatcgtaa tagaagagat acgaatattt ggcaatttgt ttttgatggg aaaaataaaa
    12001 ttgtaggaaa ataaaaagga gctgttgttt gtggtagatt acaaaacgtt tgaccctgat
    12061 ctttgggctg cgatcgccaa agaagaagaa agacaagaac ataatttgga gctgattgcc
    12121 tctgaaaact ttgtctccga agcagtgatg gctgcacaag gaagtatctt gacaaataaa
    12181 tacgcagaag gttatccagg gcatcgctat tatggtgggt gtgaatttgt cgatatcgtt
    12241 gaaaatttag cgattgaccg tgcaaaagaa ttattcggag cgaaatttgc gaacgtccag
    12301 cctcactccg gctctcaagc caacacggct gcttatctcg ctttggtaga accaggggac
    12361 acgatcttag gaatggatct ttccgcaggc ggacatctaa cacatggatc accagtcaac
    12421 ttcagtggca aaacctatca ctttgttgct tacggtgttg atccgacaac agaagttatt
    12481 gattataatg tggtacgtat tttagctaga aaacatcaac caaaattgat cgtagcaggt
    12541 gccagtgctt acggacgaac gatcgacttt gcgaaattca gagaaatcgc agatgaagta
    12601 ggcgcaaaac tgatggtaga tatggcacat atcgcaggat tagtagcagc aggacttcat
    12661 cctaatccag ttccttatgc agatatcaca acgactacca ctcataaaac attgcgtggc
    12721 cctcgaggcg gcatgatttt gacaaacgat gaagcattag caaaaaaaat aaatagtgcg
    12781 gtatttcctg gtattcaagg cggccctcta gagcatgtga ttgcgggtaa agcagtagca
    12841 ttcaaagaag cattagatcc tgcatttaaa gaatatagtg aacaaatcat cgccaatgca
    12901 aaagcaatgg tcaaagtttt caatcaagca attggaacca gagtcatttc aggggcgaca
    12961 gataatcatc tgatgttgat cgatgttcgc gagttaggta taaatggaaa agaagcagag
    13021 agcattttgg acagtgtgaa tatcactgta aataaaaact cgattccttt tgaaacacta
    13081 agtccattca aaacaagcgg tattcgaatc ggcacgccag caatcactac tcgcgggttc
    13141 aaagaagaag atgctgtaaa agtggctgaa ctagttgtca aagcattaca agcaaaagat
    13201 gataacgctc aactagacga agtcaaaaca ggtgtacgcg aattaactga aaaatttccg
    13261 ttgcataaaa aataaaaatt taaattccat tagtaaacag aaaagttcaa tgttgggctt
    13321 ttctgtttat tttttgggaa aacggattct ttgacaatat cggtgatgct ctctagtata
    13381 tgaggtgtaa ttttagtctt cacactaaat ctcagggagg aaaagtcatg actgatcgta
    13441 tctcgttaga aaaggaagca gtagcattca gtgatgcaaa tgcgcctcat ccgcgtatct
    13501 atgaactagc accggaagaa ggaagagttt tactagaaga agtacaagaa tcacctgtga
    13561 acaaatattc cgtcgatatt gaagacttga tggtggatac tggcgaatgg ggaaaactca
    13621 atattcgtct cgtacgacca gaagggaata tggataaatt accagttatt ttttatattc
    13681 atggagcagg ctgggttttt ggcagtgctc aaacccatga taaattgatc agagaacttg
    13741 cggttcgtac gaattccgtc gttgtctttc cagaatatag tcgatcacca gaagcaaaat
    13801 atccaacagc aattgaacaa aattatgctg tgttgcaaca gctgaataaa ttagccaagt
    13861 caaaaaatct ggatctcgag aatctaatcg ttgcaggaga ttcagtaggt ggtaacatgg
    13921 ctacagtcat gacgatctta acaaaagaac gaaagggcct cccaatatcc aagcaattgc
    13981 tgtattatcc agtcacaaac gctgattttg atacagattc ctatcatgaa ttcggagaaa
    14041 actatttttt gacaagagaa ggaatgatgt ggttttggga ccaatatacg acgaatgaag
    14101 aagagcgtgc tgaaatcaca gcttcgcctt tgagagcaaa tataaatgaa ctaaatgatc
    14161 ttccttcagc aatgatttta acgggagaag cggatgtatt aagagatgaa ggtgaggctt
    14221 atgcgcgcaa acttcgtgat gcaggggttc ctgttacaca agtacgcttc caaggaatca
    14281 tccatgattt tgtcatggta aatgctatgg atcagacaaa ggcaacgcgt gcagcaatgg
    14341 cattatctac ttcttggatc aagaaaaaag aataagcttt gcttgtccgc aggaaaactt
    14401 cttttcaaaa acggattttc ttaggaaaaa tggcgagttt gactagtcaa tcacttgatt
    14461 ttgttttaca attaaaaaga aacgacagaa caaaggagag cttaaaaaat gggcaaattt
    14521 caagtgatcg atcatccatt gattcaacac aaactaacta ttattcggga caaaaactgc
    14581 ggaacaaagg tttttcgtga agtagtcgat gaaatcgcta tgttgatggc atatgaagta
    14641 tcacgggata tgccattgga agacatcgtg atcgaaacac cgatcactga aacaacacaa
    14701 aaaacgttgt ctggtaaaaa agtagcgatt attccaatct tgcgcgcagg tattggaatg
    14761 gttgacggta tcttggaatt gattccagct gctaaagtgg gacatatcgg tctttatcga
    14821 gatgaagaaa cattggaacc acatgaatat ttcgtcaaac ttccagaaga cattgatgca
    14881 cgtcagttgt ttgtagtaga tccaatgttg gcaacaggcg gatcagcaat catggcaatc
    14941 gatgcattga aagaacgtgg cgcgtcgaac attaaatttg tttgtttggt agcagcacct
    15001 gagggagtaa aagctttgca agaagcgcat cctgatattg acatctatac agcttctcta
    15061 gatgaaaaat tgaatgaaca tggttatatc gttcctggac taggggatgc gggtgaccgt
    15121 ttgtttggta caaaataagt aaatacgaaa aagggtgtga cacaactttt gtcacatcct
    15181 tttttggtca tttttaacgt ttgatgcaga acagcttttt cacagctttc gtttgcatat
    15241 aaaaaaacca tcccaaaaat gcagagatgg tcaaattcaa atctttttta gttctttgta
    15301 gttaacgatt actacaccct tactttgcgt catcaagttc gcattttctg cagtttcatc
    15361 aatctcaaca acagcggaat ttttcaattg ctttgaaatc gttccagttt gcgacttgcc
    15421 gtgaattgtg aacattacgt gagtattgat cttgaatttt tcaccaattt ccgatggtac
    15481 gatagttgta tcaaattttc caaaagttgc cattttcata aaacctgcct ttcacatcaa
    15541 ttataccacg ttttatgaaa aatatcatga aattaaacta attttccatc ggactcttta
    15601 caatcagcca aatagagtat ataatttctt ttggataagg ccagcttagc tcagttggta
    15661 gagcaacgca ctcgtaacgc gtaggtcgta ggttcgatcc ctatagctgg cattaaaaga
    15721 gaagacagat atatcaagga tttttaatcc attgatacat ctgtcttttt gtttaaagga
    15781 aataaagtat gctttcttag caatagagca tgtattattt ctataaaaat ttaggagaat
    15841 aaaacgacta gcaatatagc tgtgaaatca aatatcacat gaactgccca acttgtaatg
    15901 atcgagttgc ttttaatggc agcttggttg aataacaatc ttgccacccc ttgtatcaaa
    15961 aggatgtgtg ctacagtgtg taaaatattt ccattatcat aagtactgta atggataaat
    16021 ccaaagatga ttgctgacaa gaaactagcg acaggaatgc tgaacttact agaaaaaata
    16081 gcgagaaaat acactgagaa caattcttct ccaagtatca tgataggcaa aataataaat
    16141 aataacgggc tgaaagcctc atttattgga ttgcctgtta aaccccattt caaaatttgt
    16201 tgcaaaaata ccgagactgc ggcactgatt actacattga taaaaaaata tttggctata
    16261 tttttgacag gagcgacagg tttcgagaac atttttttcg tatctttttt accaaaaaca
    16321 agtgtaaatg ctataaatcc agtgatagtt aaagaaaaaa tgctaataat ggatggcgca
    16381 aatagcaacc ctaacaaacc gagtacaacg acaatagtgc ccaatatccc ttttttaagt
    16441 tctatttttt ttcctaaaat gttattcatg ataataactc ctactattca gtttttgaag
    16501 atagagtcag tatacatgaa gagttaaaat aggcaatgtt tttttatgga tatttgtatt
    16561 gctaaaaatt tatcttatat attcattttg attatactac ttttatttgt aaaaattttc
    16621 tagcaaaacc aagtgcttgc aactgattaa gcgtacaatt ttttagatgt tccgagcgta
    16681 cttcaatcat ttcaaaggag ttgctactta aatcgatatt ttttaaatca gtatcaaaaa
    16741 agctagcatt ttctaaatca ttagtagaaa acattatttt agtgagggaa cagtctacaa
    16801 aatctgcgct tgataagaca gagtgctcaa aggtactttc ttttatgttt gaaccagata
    16861 aatttacata gtccatttta caattctcga ataacgtgtt cccaatataa ctttccatca
    16921 atttagtacc tgttaattta caattgacaa atttcacgcg atgcaacatg ctttttgtca
    16981 atatacagcc tgaaaaatca cagttttcga atatacagtc ggtaatctcc attctttgaa
    17041 aatcattgga agcaaaaaat gcatacttga aaatagattt gtaaataatt ggatggcaga
    17101 ttatttggtt ttcgatgacc tctttttgtg aaaggatatt atacagttca aaatcttcgt
    17161 tttcatagat atgaaaaaag gatcctttat tcaagcttgc ttcattaact ttaggaaaaa
    17221 tcatatttaa ttacctccat tttcataaag tcatattaac aaaagctaaa ccaacttaca
    17281 ttagcagaaa atgagatagg ttttcgcctt tttcgataac gatagtgtcg ctatctcttg
    17341 aaacgttctt agaattgccc tgagagacac aaattaggcg taattctaat aaaaatctta
    17401 gaataaaaat ttttactgat ttaatttaga atgtttttaa aataaaaatc gttgccataa
    17461 cagtgtattg aaaggtttta acaatccagt aatgatttaa aaaaatctag atgtagcgta
    17521 tactttgttt atagaatgta gtgggaagaa atatattttt gggaggaaat cgtgatgtct
    17581 aaaagtgaag aaatagaaaa catggtacta aaattatatg atctcgtttt aaatccagag
    17641 atcaaagaaa aggaacgtat acttttgata gacgcaaaga caggcttaga aaaaggtcag
    17701 tattatccta aagtcataaa taatttggaa cgtagtttac gtccgttagc aatcagggga
    17761 gaattgtcta aaccagtatc accgttttat atggagatat caacaatagg aaaatttgaa
    17821 aaagaattag gtcgaggaat ggcttcggca ccgattactt ttgggcattt ataaaaacga
    17881 gttattcatc tcaatgaatt aaaaagtata gtcaatctca taatgcaggc taggttcatt
    17941 tatttgaaca gagtctgtat ttttttattc taaataaaga aacagatgtt tctttatatt
    18001 gtattttatc ctttaattaa cacaaaaaca gctagtataa actaataatg ttaattaaaa
    18061 gtgtatttta tgaaactatc gttatttatt aatgttgcat ttttgtaagt taaaaattgg
    18121 aaatatagat tgaaaaaatg aaagcaataa gtaaaataaa atgaatatat gcgaaaaaat
    18181 aatttacatt aggaaaaacc ttgtaaaaac agttttttgg catatttatt ttttataaat
    18241 atttgtaaaa taactatcaa ttttttagtt aaaagattgg aaatgagtgg gggaggaatt
    18301 gaagaaagga tttttgagag atgatatagt aggttttatt tttagttaaa ataggggaga
    18361 tagaaaaaat gaaaaagtgt agaaatatta ttttatcaat gttatttatt attactaata
    18421 taacatcact gattcctgtt catgtctatg cggatgcagg cagagatatc agcagtaatg
    18481 tcacttcgtt gaccgtggac ccaacgaata ttacagatgg aggaaacata aaagtaaaat
    18541 tctcgtttga cgaaaaaaaa caaaatatcc aaccaggtga ttatctttgg atcaattggc
    18601 caagtgaagg aaatattcgc ggtgaaggat tccaaaaaga aatcccatta atgattgaaa
    18661 ataaaaatgt tggaacttta acggtcagaa aagattctgc gcaggttgtg tttaatgaaa
    18721 atattaaaaa cctagactca gtagaaggtt gggggcaatt tgagatacag gccagaaacg
    18781 taaccgatac cggtgaagaa aatacgggac cattcaccgt gacgagcggt gataaaacag
    18841 ctactgtaaa tgtgacaaag ccagcttctg gttcttcttc tagcgttttt tattataaaa
    18901 caggagacat gctacctgaa gatactaagc acattcgatg gtttttgaat attaacaata
    18961 acggcactta tgttgaacaa ccagttaaaa tcagtgatga aatccaaagt ggacaaagat
    19021 tagatccaag tacgtttgaa attaatcaaa tacacttagg tgaacagaag gtttatagag
    19081 gagaagaggg aatccaacaa tttttacaag atttccccag cgctacattt aatttttcag
    19141 taactgataa ttacattgaa ataactatac caaaaaactt tgtcaatctt aggaaaataa
    19201 tggtgagtta taagacgatc atagaaaacc cagaacaaat aaattttgag aatcattcgg
    19261 aagcttggtt taaagaattt aataaacctg ctgttgatgg agaaagtttc aatcatacag
    19321 ttaaaaatat ttcagcttct ggtggagtca acggtacagt tagaggcgaa ttgaagattt
    19381 tcaaatatat caatgacaca gagattggta tcccaaatgt gactttcgaa ttaagacgag
    19441 cagatgaaca gcctattcaa ggacagtcta gtatcctgtt gacttcgaat gaacagggag
    19501 aagctagtat caaaggcctg caagtaggag actatgttgt aaaagaaaaa gaagcaccca
    19561 attggattga ttttgatcca ctaagttcaa atgaactcaa attctccata aacgaaaatg
    19621 atacagaggg agtcagttta cctatttata acaagaaaaa ggttacgaac atcacagcaa
    19681 ctaaaatttg gaacggtgga accactccaa gaccgagtat ttattttaaa ttattcagag
    19741 caagtacaaa caattgggaa ccagttcctg atgcagaaac aaaacgatta gacaatggaa
    19801 taacttcagt gacttgggaa gatatccaac agtatgatga tagtggaaac gaatatacgt
    19861 ttaaagtgca agaagtagat caaaacggaa atgattatgt cccttcaggt tatcaaaaaa
    19921 ttgaaaacgg gctagtcgtt acaaatgaga ataaagaagt catttcagtc tcaggacaaa
    19981 aaacatggga agacaatgag aaccaagacg gcaaacgacc gacgacgatc acagtaaatc
    20041 tattagccga cggtcaaccg atccaacaca aagaagtaag tgaaaaagat gattggcgct
    20101 atcgtttcac aaatctgcca aaatacagag acgggcaaga aatcatctat actgtaacgg
    20161 aagacaacgt gcccgaatac agtacgacga ttgacgggta taacatcaaa aacagttata
    20221 caccggaaaa aacaaatata tctataccag gtagccatat aacaccgtgg aaaaattttc
    20281 cagagaagac agagagagag aaaattagaa atctattctc tcaaaaattg caaatatttg
    20341 ctcatacagg agaatccagc gaacaaagaa gcaattatat tagtaagaga gctgtaccta
    20401 aacagataac aaataaatca aaaagcattc ttcctaaaac aagtagtaaa aaatctagtt
    20461 ttgctgtaat cattggttta ttgatagtaa ctatctgtgc tgggatattc ttacaaaaat
    20521 ataaaaaagt ttagttagaa tataatattt tttgttaaat atgactgaaa tcaaaagaat
    20581 aaaattccga tttataatcg aaaaaagaag catttttata tatgcttctt ttttcgtaga
    20641 atcaataaat atggatttgg ctaactatgg aagaatactc gtattagttt tcagcgttca
    20701 tactagttgt tacattgttt gattggccaa aagaatttct gatatatttt gtgctaaata
    20761 aagaaaaaga cgcttgaatt gggaaataag aaaactactc tatcattaca aattttcata
    20821 aactggcaga tgttctaagt aaacaagtat cttttcaaag tcggaaaaat ccataatcaa
    20881 cgtagtggta ttgatatttg gatgaaagcc tacggtatct tgacgatcaa tagcagcatc
    20941 gatcacgact tggatttttt tctctttgtc gtgcatcaat gctaatggag tcactgtacc
    21001 tgctggaacg cctaggaggt caggaagctg ttcttcagaa acaaaagata agcgcttttc
    21061 ttttaattgt tcagctagtt gttttaaatc ggcgtgcttt tcatcaggca agatgacaag
    21121 atagatttgt tttccttttc ttgatttcaa taatagattt tttacttgcg gaccaggtag
    21181 ggaaaaaggg acatttttga ctgacgtaat cgcaggatga tcgacttttt gaaaagagat
    21241 acccagtttt tttagtagtt cataagcttc ttgttcggtt gcacgactca tcgcgcatca
    21301 ccctcctttt tttattagtg tatcataaaa aagagttgac aaatgaaaaa taatcatgta
    21361 aattacacgt aaagttaaaa caaatgacat cgaaaagaag agtagccgtt aggtgttttt
    21421 agagagtctc tggttggtgg aaagaggcag cagttaaccg tgaaacacat cttggagtcg
    21481 attttctgaa gcgtatagta ggaaaatccg gggagcccgt tacagctgcg gtcgttgcta
    21541 gatgaccaga taaggctggc ttttccgtga ggagtcggta aaaaaaggtg gaaccacgtg
    21601 attaacgtcc tttgatcttc ggatcaaagg actttttgtt ttcatctgaa cggcaacgac
    21661 tgtgtgaggt tttttctgca aggaaaaaat aaacagaggt ggaaccacgt ataatacgtc
    21721 ctcttggcat ttgccaagag gattttttta ttttaaagga ggaaaagaag atggagtatt
    21781 tgattgaagt tatgccgcaa ttattagtag gagcactcac aacattgaaa ttatttttat
    21841 ttaccttgat tggctcatta ccattaggga ttgttttagc attaggattg tctttgcatt
    21901 ttaagccgct acgctacttg cttcaaacat atgtatgggt tatgcgggga acaccgctgt
    21961 tgttgcaact gatttttatt ttttatgggt tgccgacgat tgggatcgtt tttgatcgtt
    22021 tcgaagcagc actcattgcc tttatcttaa actatgcagc ttattttgca gagatctttc
    22081 gtggagggat acaatcgatt cctcaaggtc aatatgaagc agcgcaagtt ttgcaattaa
    22141 gtccctttca aacggtgacg aaaatcgtca tcccacaagt gacaaagatt gttttacctt
    22201 ctgtagggaa tgaagttatc aacttggtca aagattcttc cttgatctat atattaggtt
    22261 taggtgacgt aatgcgtgct gggaaaatcg cgatggaacg cgacgcgact ttactgcctt
    22321 tgctaggcgt ggcaatcatc tatcttacat tgactggtat tctaacaatc ataatgaaac
    22381 aagttgaagg aaaattaaat tattaccgct aactgatagg aggaagaaat atgattgaat
    22441 tgaagaaagt gaacaaagca tttggtgaaa aaaaaataat caaagatttg aatctaatca
    22501 ttccagatgg gaaaacatta gctatcgtag gtccttccgg tggcggtaaa accacgcttt
    22561 tacgtatctt ggctggctta gaaagttctg acagcggaga attttattta gacggtgtac
    22621 cgttcaaccc attcgagctt aaaatgaaag aacaagttgt aggagtcgta ttccaagact
    22681 tccagctatt tccacatctg aatatttttg aaaacattac cttagcacca aaaatggtat
    22741 tgaaacagac aaaagaagaa tacaccaaaa aagcagaaga attagcggct tcattaggga
    22801 ttagtgattt attgaatatc tatccttatc agctttctgg cggacaaaaa cagcgggtag
    22861 cgattgccag agcattggct atgaatccac gggtattggc ttatgatgaa ccaacaagtg
    22921 ctttagatcc tgaattacgc cagcaagtcg agcgtttgat cctatcattg aaagaagatg
    22981 gggtaaccca aattattgtc acccatgatt tagtatttgc agaaaatgtc ggtgatcaat
    23041 tactaaaagt ttccccaatt cattaagaaa ggagaagata gagatgaaaa aaaccagttt
    23101 tctattcgct ttgatcgcag gtcttcttct ttttgcaggc tgttcgaaca aaaaaacttc
    23161 agcagataat tgggcgacta tcaacgaaga gaaaaaaatc gttataggtt tagacgatac
    23221 atttgttcct atgggattta gagataaaga cgggaagctc acaggatttg atattgattt
    23281 agccaaagca gtctttgaac aatatgggat tacggtagac tttcagccca ttgactggtc
    23341 aatgaaggaa tttgaactga acaatggaac gatcgatttg atatggaacg gctattccaa
    23401 aacgccggca agagaaaaga aagtgcaatt tacaaaacct tatatggaaa acgatcaagt
    23461 gctgatcaca cctaaagaat caggaatcac aaactttgga caaatgaata ataagagatt
    23521 aggtgcgcaa aatgggtcat ctggttatga tgtatttacg aaacaaccag aagtattaaa
    23581 agagatagtc aaaaaccaag atgctgtttt gtatgatagt tttaatgaag cattgatcga
    23641 tctgaaaagc agacgggtcg atggtctgct gatggataaa gtatacgcag actattattt
    23701 gaaacaaaga aacgaactag aagacttcaa cataacaaaa ggttcttatg agagtgagga
    23761 ttttgcagtt ggtgcacgca agtcagatag tacattagtg aaaaaaatca atgaagccct
    23821 agatcaacta gagaaaacag gtgaattcca aaaaatatcc gataaatggt ttggcgaaga
    23881 tgttacgccg agaaattaac aaatagggga ctgtgacaaa agacgtgtca cagtcccttt
    23941 ttccgaataa acagtgttct cctgccaact ccttgtgact cggagctgaa tggcaggctc
    24001 acaacctctt ggtaaacggt gttcaaaagc cagtttttcg gtattaagcc aaatttatgc
    24061 aaaaatgtag aaagccattt tcacaaaatt tttcttacta ctcgaaacta agcgactttt
    24121 tcacaacctc ctttacataa ttttattttg ttgtggaagg ggtttcgtgt tattctaaaa
    24181 gaggaacgat tgaaggttat aggaaaggaa gagatgaatg gaacacaaag aacttacctc
    24241 atttccaaaa gattttctgt ggggctctgc ttctgcagcg tatcaagtag aaggagcttg
    24301 gcaagaagat gggaaaggag aatcagtctg ggatcgattt gtacggatac ctggaaagac
    24361 atttaaagga acgaacggtg atttggcagt agaccactat catcgttata aagaagatat
    24421 tgctttaatg aaagaacaag gtctgaaagc ttaccgcttc tctgtagctt ggacacggat
    24481 ttttcctaat ggacgaggag aagtcaatca agctgggctc gctttttatg agagattgat
    24541 cgacgaattg attgaaaacg atattgaacc tatgttgact ttatatcatt gggatcttcc
    24601 acaagcattg caagatgaat ataatggttg ggaatcacgt cagatcattg aagatttcac
    24661 tcattatgca gaaacattat ttgaggcatt ccgcgggaaa gtccattact ggattagttt
    24721 gaatgaacaa aatatcttca cttcattagg ttatttgtta gcagcacatc cgccaggtgt
    24781 gacagacccg aaacgaatgt atgaagttaa ccatattgct aatttagcaa atgcatctgt
    24841 catcaataaa tttcacgagt tagaaatgcc tggaaaaatc ggaccaagct ttgcgtatac
    24901 gccaaattat ccaattgaca gtaatcctga aaatgttctg gctgctgaaa atgcagaaga
    24961 cctgatggcc aattattgga tggatgtcta tatgtgggga aaatatccga ttgctgccat
    25021 gaggttctta gaagaaaaag gatgggcacc aacgattgaa gctggcgatg cagaattgct
    25081 agaatctgct aaaccagatt ttcttgggat caattactat caaacggcga cgaatgcatt
    25141 caatccatta aacggagttg gtgctggaaa aatgaatacg acaggaaaaa aaggttcatc
    25201 agaagaaact ggagttcctg gaatgtataa aaaagttgag aatccatttg tcgaacgaac
    25261 aaattgggac tgggagattg acccagaagg attaaggatc ggactgcgtc gtatcacaag
    25321 ccgttaccgc atgccggtac tgattacgga aaatggctta ggagagtatg acaagttgac
    25381 agaagataaa caaatccatg acgactatcg aatcaattac ttacaaagcc acgtcaaagc
    25441 catcaaagaa gcaatttcgg atggagcaga agtcctaggc tattgcacct ggtcttatac
    25501 agatttgctt agctggctga acggttatca aaagcgatat ggatttgtat atgttgatca
    25561 agacgaaaca caagaaggat ctttggaacg atataagaaa gacagttatt actggtacca
    25621 aaaactgatt gaagaaaatg gagagaacct ttagttcaca tacttaaaac aagaaggtac
    25681 ggctggatat ggccgtgcct tttcttgata aaatcattta gaaattaaga aaaactgtaa
    25741 aataagctga aaaatgaaag aaagcgtcag ttctttcaaa caaaacttta gttcccttac
    25801 ttttttttgt ataatgaaac ttgatgaaat gagggataat aaatggcaca aaagaaacgg
    25861 cctactaaaa aaaggaaaaa gacgaaaaaa acgaaaaagc aaaaacagca gcaggaacaa
    25921 atgctgacga ttggtgttgg gtttgccttg attttatttg ctgtcttcgg ctttttaaaa
    25981 ttaggttttt taggaatatt gattgcaaac ggctttcgga tcattgcagg aaatacttac
    26041 caaatcctct gtcttttatt agccgtatta ggattttgga ttgtcattaa gaatacagag
    26101 ttttcaattg gtaaaaaccg acgctggttt ggcggcatac tcttttattt cggtatctta
    26161 ttattgttac atgcccattt atttggcaaa ctccatactg gggaaccaaa tatcatgggg
    26221 acgacatggg atttgttagc cagtgatata aaacaaagtc aagtagacaa taacgttggt
    26281 ggtgggatga ttggagcaat cctttatcat tttacttact tcttgattgc acagcctgga
    26341 agttatttag cagcagtgct attactggca ggaggagcat tcctgttcag taatctggaa
    26401 ggctatcagc tgttaaatgg tatccaatca attggagaac gagtacagga attattggaa
    26461 ggtgatcctg ccaagcaagc tcgaaagcaa gcggcaaagg aagagagaat gaaacagcgc
    26521 gcagaagcga aagaagcaag gcgcttagct gcaaaagaag ctgcagaaaa agaagctgca
    26581 gaatatgaga agaaaaaagc ggtgagccag caaagggaaa atactgctga tgaatggcag
    26641 gaaccagaaa atacagagcc ggagcagcta agttttgttc caatcgacag ttttcaagaa
    26701 aatatccatc ctgcgaatct agagaaacca gtaccagata caccaaaaca gaccaataca
    26761 gcagaaggtt tttcagatga gttgccagag gatgacggta cctcattaga atttgagatt
    26821 gaagcagaac aggaaaatca agactatgaa ttaccgacag ttgatttgct tgattctata
    26881 ccaactgtgg atcaaagtga tgagtataaa aaaattgaaa aaaatatcgg cgtattagaa
    26941 caaactttcc aaagtttcgg tgtcgatgcc aaggtcgtca aagccagctt aggaccatcc
    27001 gtcacaaaat tcgaagtcca gccggctgta ggggtaaaag taagtaaaat tgtgaactta
    27061 acagatgata tcgcgcttgc tttagcggcg aaagatgtcc ggatggaagc accgattcca
    27121 ggtaaatcac tgattggtat tgaagtacca aatggaaaga tcagtatggt ttcctttaga
    27181 gaaatcatcg aagcacaacc aaaccaccca gataaattac tagaagtacc tttaggacgt
    27241 gatgtttctg gacgggtaca aacggctgat ctttcgaaga tgcctcactt actcgtggct
    27301 ggttcgactg gtagtgggaa gtcagtagca attaatggga ttatcacaag tatattaatg
    27361 cgtgctaagc cccatgaagt aaaactgatg atgatcgatc cgaaaatggt tgaattgaat
    27421 atgtacaacg gcatacctca tctgttgacg ccggtcgtga cgaaccctag aaaagcagcg
    27481 caagccttac aaaaagtagt acaagaaatg gaagaacgat atgaaaaatt cgcggcaacc
    27541 ggagtaagaa atatttctgg ttacaatgaa tttgttcaac agaaaaactt ggaaaacgga
    27601 acgaaacatc caaccttacc attcatcgta gtgatcgtag atgaattagc tgatttgatg
    27661 atggtagcca gcaatgaagt tgaagatgcg attattcgtt tagcgcaaat ggctcgggca
    27721 gcggggattc atatgatcct agctactcaa cgtccaagtg tggatgttat cacaggaatc
    27781 atcaaagcaa atgtaccttc cagaatggca tttgcggtat caagcggtac agattctcgg
    27841 acgatcattg attctaatgg ggcagaaaag ctcttagggc gaggagatat gctattcttg
    27901 ccaatgggtg agaataaacc gatccgtgtg caaggagcat tcatttctga ccacgaagta
    27961 gaacgggtcg ttcaatttgt tacagatcag caagaagctc attatgaaga gaaaatgatg
    28021 ccgacagatg aagttgaaac gacaggtgct ccagaacagc cacaagatga gctgtttgaa
    28081 gaagcaaaag cacttgtcgt tgaaatgcaa acggcaagta tttcccttct ccaaagaaga
    28141 tttcggatcg gttacaaccg agcagcacgt ttagtagatg aactggaagc tcatggagtt
    28201 gttggcccgt cagaaggaag caagcctcgg aaagtattca ttgagcagga agaaacatca
    28261 gaaacgttgc cggacgataa tgacatgtaa aacttgtgtg atgaagggtt gaaaaacgaa
    28321 taaccaataa aaagacaagg catgatgata ccaaaagaaa aaatggtttt tttccatctt
    28381 ttctaatacg gtatgatgtc ttgtcttttt agtttttgtg agattaaagt tctattcgat
    28441 ttatttgtga agtattgcat taaggaaatg aatcatgtat agtatgtagc gaaactttga
    28501 gaaaaagagg gaaaatactt tggaaaaaca gtccatttat ggcttgacga atgaagaatt
    28561 aatcaattgg tttatagaaa acggcgagaa aaaattccga gctgcccaag tttgggaatg
    28621 gttgtatcaa aaacgtgtaa gtaatttcac agaaatgacg aatctatcga aacaattgat
    28681 cgaaaaatta tcggagcatt ttatcatcaa tcctctaaaa cagatggtcg tccaagaagc
    28741 ttcggatggt acagtaaaat atttgtttga actccctgat aaaaacatga ttgagactgt
    28801 actgatgaga caagaatatg gcttgtcagt ttgtgtgacg acacaagtag gatgtaatat
    28861 cggctgtacg ttttgtgcaa gcgggctgct caaaaagaac cgtgatctga ctgctggaga
    28921 aattgttgca cagatcatga tggtccaaca ttattttgat gagcgcaatc ttggcgaacg
    28981 cgtttcacat gtcgttgtaa tggggatcgg tgagccattt gataattacg acaatgtaat
    29041 ggactttttg catatcatca atgatgccaa aggattggca attggtgctc ggcacattac
    29101 agtttccact agtggattag cacacaaaat caaggaattc gctaataacg gcttgcaagt
    29161 caatctggct atttccttgc acgcgccgaa taatgaggtg cgaacatcga tcatgcggat
    29221 caaccgaagt ttcccaattg agaaattgat ggaagctgtg gatgagtatt tagagaaaac
    29281 aaaccgtcga atcacgtttg aatatatcat gttgaatcaa gtaaatgata gaccagagca
    29341 tgcgcaacaa ttagcagatc tattaaaaga taagaaaaaa ttagcatatg tcaacttgat
    29401 tccatataat ccagtcagtg aacatgatca atattctcgc agtccaaaag ctgatgtatt
    29461 gagattttat gatgtattga agaaaaacgg cgtaaactgt gtgatacgta aagaacatgg
    29521 tacagatatt gatgcagctt gcggacaatt gcgaagcaag caaatgaaaa aaacagttgc
    29581 caaataatcg tatcctactc gttttttcaa ttaccataaa aggagcagtt ttcttaatta
    29641 aggaactgtt caacttaaat aagagaaaag attctatagc gaaagctata aagctttttt
    29701 tgacaagaaa aaccctcatc aagcaaagaa atgtcctcaa tttgatgaca gcctgcttga
    29761 tgggggtttt agcaattatt taactgattt tcttatatcc ttgctggctt tcttgtgtag
    29821 catgataagt ttctacgtca agaatggcct gtttaaattt ttctcgggtg atttcataag
    29881 gcacatgttt aatatcattt gttgccatgg ctttatctaa gattgcttct aaatcggtcg
    29941 tgacattgag attcatgtcg gttaacgata ctggaagttg gttctttttc ataaacacat
    30001 agattttatc aaacaaatcg aactgctgat ccatcatcaa taaggtcagt acaccataac
    30061 tgacagatgc tccgtgaagg tatccctcca ttttttctag aacagtcgta ccgtaataga
    30121 atgagtgtgc catagcagaa ttatagttat tgttaactaa aacagaaacc aatccggtag
    30181 taacaatgat atccgaaacg acttgttcaa ttgcatatga gggacgatcg tcggcactat
    30241 ctttcaatgc ttgcgtacca taagtcaaga gacgatcaat gcacattttt ccgatttgga
    30301 ttcctaatct atccgtgtag cctagatcat cgccacgagc agaaaaagta gattcgactt
    30361 ctttggataa agcatcgcca atacctgccc ataagtattt atcaggtgct tcggcaataa
    30421 ttgctgtatc aatgaaggtg tgtacaggtg gttttgaagg aaagaagagt ccgtccatta
    30481 cgccttcttc gttatagatg acacataccg cagtgacggg cgaacagttc gaaccaatag
    30541 tgggaaaggt gaagactggt ttagctagtt tttcaccaat gacttttaca gtatcgcaag
    30601 cacggccacc accgactgca aacaggacgt ctgctttttg gacactttca tgctggatca
    30661 actgttcaat atttccataa gttgaattac ccccgtacca gagactggcg acaacctcta
    30721 tttcctgtgt caaagctact tttattttgt tttctgcctt tgaaagtgca gtcttccctc
    30781 caatcagcac tacttttgtc ccatattctt tgacgattcc atttgctttt tcatacgcat
    30841 ctgttccaat ggtataattt ggtaagaaaa cactttgact catattcatt tccttctttc
    30901 ttcgttgaaa cgggttaaaa ttttaaagag tgatcgatct gactgcggct gtagatggag
    30961 tagaggtgaa cctaagacag gataagtgat gaataaggtg gcatgaacac cttgaaatca
    31021 tcaataagta tcctaaaaac gaacaagtac aaaaacattg aaaacgacaa taatgaattt
    31081 ttgttcctct tagtatttct accgattccc tttgcttttt tgtaattatc taaaaattct
    31141 aaaaattaag attattatat gaaaacaggt tttatttgtc aattcataat tgtttttttt
    31201 taaagcaaaa gataatctat atcatgatga acaaacagag ttttttcctc caattgatgg
    31261 ctttcctgat ctttcctatt ttgaataaag tgtttctgta taattttttt attttataag
    31321 taagataaat aatgaaagaa tagagatact gattggtagc taaagatttt tgagcgagaa
    31381 catttcgttt tttcaaaagg actgttagaa taagcaagaa caagggggaa gacgtatgat
    31441 aatcagtgaa tttgaccgta ataatccagt attgaaagat cagctttctg atttactgag
    31501 actgacttgg ccggaagaat atggagacag ctcggcagaa gaagtagaag aaatgatgaa
    31561 tccagaacga atcgcggtag cagcggtaga ccaagatgag ttagtaggat ttattggtgc
    31621 aatccctcaa tacggtatca caggttggga attgcatcca ttagttgtag aaagctcccg
    31681 acgaaagaac caaataggta ctcgattagt caattactta gaaaaagaag tagcttccag
    31741 aggaggaatc acgatttatt taggtacgga tgatttagac catggaacaa cgttaagtca
    31801 aacggacctg tatgagcata catttgataa agtggcttcc atccagaacc ttcgtgaaca
    31861 tccgtatgaa ttctatgaaa aattaggtta taaaatcgta ggtgtcttac caaatgcaaa
    31921 tggctgggac aaaccggata tttggatggc aaaaacgatt attcctcgac caaattctca
    31981 ataataaaaa gaggttgtga cagaagtaat cagcttcaag aaataagaag gaatttacga
    32041 aaattacttc ttgaattttt gtgaaattcc aacttatttc cgaagaagct acttttggaa
    32101 caccgtgtac cagaggttgt gaaaaagtcg cttagtttcg agtagtaaga aaaattttgt
    32161 gaaaatggct ttctacattt ttgcataaat ttggcttaat accgagaaac tggcttttga
    32221 acaccgttta ttcagataag ggactgtgac aagacttttg tcacagtttc tttttgttta
    32281 ttctttttta tctttgtatg gcaataatag acaatataac gaaaattatt taatggcttt
    32341 tggaagacca tggaaaacct tgtacaaagc tattttttct gaaaaattcc ttgaaacctc
    32401 acgaaaaaat cattatttta tgaaaaagac aaaaaaaagt gttgattttt taataattat
    32461 tcattaaact cttaaacaac ttcagaaaat attaattatt ctgaaaattc caataaaggg
    32521 gtacgtaaaa tgaaaaagaa atttacattt ggtttggttg cggtatgcgg ccttgtactt
    32581 gctggatgtt atggcggcag ttcagccaca agcagtagtt cagcaaaaag cggttcagca
    32641 gatggaggag gcgtatttaa cttagtcgtg cctcaagaaa tgcctacagc tgacttatca
    32701 gttgcaacag atacaatcag ttttacagca ttgaataacg tttatgaagg aatttaccgt
    32761 cttgatgaag acagcaaacc acagccggca ggagctagcg agttggcaga agtcagtgaa
    32821 gacggattaa cttacaaaat caaattacga gaagacgcaa aatggtcaaa tggtgatcct
    32881 gttacagcag cggattacgt ttatggctgg caacgaacag tagacccagc tacagcttct
    32941 gaatatgctt acctgtatgc gccagttgaa aatgcagaag acattacagc tggaaagaaa
    33001 gataaatcag aactagggat caaagcagtt ggtgactatg aattagaaat caaattgacg
    33061 aaacaaacac catatttcca atatctgctt gctttcccat catttttccc acaaaaacaa
    33121 tccgtagtgg aagaaaatgg ggatgcctat gcatctgcaa gtgataaagc tgtttataat
    33181 ggaccattca cattagaaga ttttgatgga ccaggaacag atacagaatg gacttataag
    33241 aaaaatgatc attactggga caaagataca gtaaaacttt ctgaaatcaa agtcagtgta
    33301 gtaaaagaat cttctactgc attgaactta ttcaaagatg gccaagcaga tgacgtgatc
    33361 ttgtctggtg agttagcaca gcaaaacgca aacgatccag cttatacttc tgttaaagaa
    33421 gcgcggacaa gctatatcga attcaatcaa cgtgaaaaga attcaccatt caacaatgtg
    33481 aacctaagaa aagctatttc ttattctatc gatcgtgaag ctttagtaaa acaagtacta
    33541 ggtgacggat cagttgtctc tacaggattg atcccatcag atatggtaaa aagtccaaca
    33601 acgaacgaag actttgcaaa agaagctgga aaattagttt cttatgacaa agataaagca
    33661 aaagaatatt gggaaaaagc gaaaaaagaa ttgggcgttg attctttaga atttgatttg
    33721 atggcttctg atgatgattc atcgaaaaaa gtaatcgaat atgttcaaaa ctcaatccaa
    33781 gaaaacttgg atggcgtaac agtcaaacca acacctgttc cattctctgt acgtcttgac
    33841 cgctcaactt cgggtgattt tgacgcagta ctaggcggat gggctgctga ctatgcagac
    33901 ccaagcagct ttactgattt gtttgtcaca ggtaattcat acaaccgtgg tcgctggagc
    33961 aatgcagaat atgacgaagc agttaaagca gcttcatcaa cagacgctgg cgatgaacaa
    34021 gcccgttggg atgatttcca aaaagcaatc aagatcatct ctgatgatat gggtgtcgtt
    34081 ccggtgtacc aaaaagcaga aggtcaccta gtgaatgaaa aaatcaaagg tatcgttcac
    34141 catgcggcag gcgcttcatg ggattacaaa tggacatacg ttgaagaata atcgttaata
    34201 ccgtgagcat gagaaaaaac gaggctgcgg caaatgtcgc aacctcgttc tcatattcac
    34261 ttatcctaac aaagaaaaaa gcatgaaaat agcagaaaga gcaattagta ttccggcaat
    34321 gatcagccaa aagctgtaca taccgtagct ggcagacgag agcgaggaaa attctggttt
    34381 tttctttcgg ttccttgttc gtgcaagatg gaccgatcgc tgaaaatgat cgaagaaacc
    34441 cgaagaaaag acccataaaa aaccgccgat aatcagaaac ggcaatgacc ataagaatag
    34501 atcattagat aattgaatga gtgtaaagga atggttgatg agatttttta ttaaaataac
    34561 cagaatcagt agaaaagcta ctgcgtaggg gatagatttt tttttcattt gattgctcct
    34621 ttatttttgg ttcattatct aataatgtag cgaaacatct gtcagaagtc aaaagctagg
    34681 gggaaacatt agtgaatagt tatatcaaat atgtattaaa acgtgtcttt ttcatgatca
    34741 tcacgctttg gttaattgca acaatcacct ttttcttgat gcaattgctg ccgggcacgc
    34801 catatacgaa tcaggaacgt ttgagtcctg aaacaatcga gatgctaaat aaacaagtcg
    34861 gtttggataa accagttatc gtacaatatg ggatttatct ttctaatttg cttcaaggag
    34921 attttggaat ttccttccaa tttaaaaacc agccagtcgc acatttgtta gctggaagaa
    34981 tcggaccatc cttgcagtta gggttacagg caattatctt tggaacagtc ctagggacga
    35041 ttttaggaac gatctctgcc atgaagcaaa atacatgggc agatacatct tctacactcg
    35101 tagcgatatt aggtcgttcc attccaaact ttgtatttgc tgttttatta caatatatct
    35161 ttgcaatcaa attacaagtt ttgccaatcg caaaatggga tggctttgtg tacacaatct
    35221 taccaacaat tgcgttggcc atgtcgccgc ttgctgactc tgctcggttt atccgtacgg
    35281 aaatggtgga agtactgcat agtgattatg tggaattagc aaaagcaaaa ggattgagcc
    35341 gttgggaaat cgcctttaaa catggtcttc gaaatagttt gattccattg atgactttat
    35401 taggaccgtt agctgtagca ttgatgaccg gatcactggt ggtagaaaat atctttgcta
    35461 ttcctggtat cggggaacaa ttcgttaagt caatcacaac aaatgattat cctacgatca
    35521 tggctgtaac gatcctttac tcatttatgc tgatttttgt tattttgatc gtggatctat
    35581 tgtacggtct agttgatcca agaattcgtg tttcagaagg gagccgagga taaatggaaa
    35641 tgataccaaa gcgttatgaa acagttgcag atatccctgc ggatgaattt cttccattac
    35701 aaaaaaatac cgaagaagaa cgtgaacaga ttactgcacc ttctttgaat tttatccaag
    35761 attcatggcg ccgtttaaag aaaaacaaag ctgccgttat ttcaatggtt ttactattgg
    35821 tcatcatttt gatctctgtg atcacaatct ttgtttctcc tcatgatcca actgcccaaa
    35881 acgtggctta tatcaatttg cctcctcgta ttccgggaat caatatcgat ggattgaatg
    35941 gaaaagcaat ggttggtggt gaacttgttg ataaatatgc tcaagcaaac gtcccttcaa
    36001 acgtaaattt ctttttagga acagacggtt tgggacgtga tgtattaagc cgcttattta
    36061 tgggaatgag aatctcgttg ctgatcgcat ttattgctgc attactagat gtaacgattg
    36121 gtgtagctta cgggttggtt tctggtttgt taggcggacg tgtcgataac gtgatgcaac
    36181 gttttttgga aatcttatca ggaattccaa acctcgttgt catgattttg atgttggtcg
    36241 tttttgaacc aggaattttt tcaatcgtgg ctgctatggc catcacaaac tggataccaa
    36301 tggcacgtat cgttcgtgca caaacaatga aattaaaaga tcaagaatat gttttagctg
    36361 gattgacatt aggtgaatca agaatgaaaa ttgcattcaa acatattttg ccgaatatct
    36421 ctagtgtcat tattattcaa atgatgttta gtattccatc tgctattttc ttcgaagcct
    36481 ttttaagctt tatcggttta ggtcttacgc ctccgtcagc ttcattagga acgatgttga
    36541 gcgaagggta caagacattc ctgtatctgc cttatctatt atggattccg gcagtaactt
    36601 tatcggttat catgattggc tttaacttac ttgctgatgg attgcgtgat gcctttgatc
    36661 ctaaaatgaa agagtgaaga cgatgacgaa aattttagaa gtgaaagact tagaaatctc
    36721 ttttgatacg tatgctggaa aggtacgagc gattcgcggt gtcaattttc atttgaataa
    36781 aggagaaaca ttggcgatcg ttggggaatc tggaagtgga aaatcagtga cgaccagaag
    36841 catcatgcgc ttgctgtcaa gcaacgccaa tatagatgct ggtcaaatcc tctttaaagg
    36901 taacgatatc gttggaaaat cagaaagaga gatgcaaaaa atccgtggta aagaaatagc
    36961 gatgattttc caagatccaa tgacctcttt agatccgacg atgcccattg ggaagcaagt
    37021 cgctgagtcc cttagaaagc ataataaagt ttcaaaaaaa gagggacaaa aagctgcatt
    37081 agaattgctt aaattagttg gaatacccga agctgagaaa cgtatcaaca gttatccgca
    37141 tcagttctca ggaggtcagc gtcaacggat cgtgatcgcg attgcactgg tatgttatcc
    37201 ggaaatcctg attgctgatg aacctacaac agcgcttgat gtgactattc aagcacagat
    37261 tttggagctg ctgaaagaaa ttcagacaaa aatcgataca tcaattatct ttatcactca
    37321 tgaccttggt gtagtagcga atgtcgctga tcgtgtcgct gtcatgtatg gaggaagaat
    37381 cgttgaagtt ggaacatcgg aagaaatttt ctacaacccg caacatccct atacttgggg
    37441 actgcttggt tcaatgccga cattagatag tgcgaacgat cgattgtatg ctattcctgg
    37501 ttctccaccg gatttattga acccgccaaa aggagacgca ttctatccaa gaaatgaatt
    37561 cgcgatgaaa atcgatgcag aacaagaacc accatttttt gaactatcaa aaacacacaa
    37621 ggcagcaact tggctacttg ctccgcaggc acccaaagtg acaccgccag aagaaatcca
    37681 acgtcgctgg gctatcttta aagagaaaca aaaacatact tacggtcaag gaggaattgc
    37741 tgatgccaaa taaactactt gaagttaaag gattgaaaca gtatttcaat gtaggaaaaa
    37801 agaatgaagt acatgctgta aatgacatca gctttcacat ttatgaagga gaaacattcg
    37861 gtttagttgg agaatctgga agcggaaaat ccactactgg ccgtacgatc attcgcttga
    37921 atgaacctac tgatggtgag atattattcg acggtcaaga tataacgaag atcaaagata
    37981 aaaaaggttt gacgaaattc cggcatgatg ttcagatgat ctttcaagat ccatacgctt
    38041 cgttgaatcc tcgtatgaaa gtcagagata tcattgccga aggaattgat gttaatggac
    38101 tagctaaatc atctgaagag cgtgcaaaaa aagtcgatga tctattgaga actgttggat
    38161 tgaatccaag ccacggtact cgttatcctc atgaattttc aggtgggcaa cgccagcgta
    38221 ttggtattgc acgagcattg gcagtaaaac cgcgttttat catttgtgat gaaccaattt
    38281 ctgcactaga tgtgtcgatc caagcccaag tggtcaattt gttgcaagat ctccaaaaag
    38341 aacatcaatt gacctatcta tttattgcac atgatttgtc gatggttaaa catattagtg
    38401 accgaatcgg tgtaatgcat aacggcttgt tattagagat gggaacaagt gatgaaatct
    38461 ataatcacgg tgttcatcct tatacagaaa gtttgttatc tgctattcct ctaccagatc
    38521 cagaccatga gagaaaacgc agaagaatca aatatcaacc tgaaccagat gacggacaag
    38581 tacgtcaact tcgtgaaatc gcaccagaac attttgtcta tgcaactgag caggaagtcc
    38641 catattacgc aaaaaaacta aaacgacaaa aggaatctct tctggttgct aactaggagg
    38701 gacatagatg ctaaaagctt ttaaattccg tatgtatccg acagaagaac aaaaacagca
    38761 attgattcgt acttttggat gtgctcgatt tacctataac catttgctga aaaagcggca
    38821 gaaaagttgg caacaaacag gagtagccaa tttttcgctc acacccgcta cattaaaaaa
    38881 agaataccca ttcttaaaag aagtagacag tcttgcttta gcaaatgcac agcttaattt
    38941 ggatcgggct tttcgcaatt atttcaaggg acgtgccagc ttcccaaagc ttaaaacgaa
    39001 aaaaagtatg tggcaatctt atacgaccaa caaccaaacc cgtacgatct atctggaaaa
    39061 tggctactta aagttaccaa aacaaaaaga actaatcaag ataaatcagc atcggccagt
    39121 tgaaggatcg attcgctccg ctacgatttc tgctcgatat aatgaagaat tttatgtcgc
    39181 actactttgt gatgtttcgc cagtaaaaaa agaatcttta gctaaatgga tcgggatcgc
    39241 ctatcatccg aagacattga tccaaacatc tcgacctcta gaagtaactt taccaaaatt
    39301 tcaccagaca gaagaaaagc tgcaacatgc acagcggaaa ttgaatgtga aagtccgctc
    39361 cgctcaccat agaaagatca ggttagatca ggcaagcaat tatcagaaac aaaagagaaa
    39421 agtaatggat ctttatctga aacaaaagaa ccagcgagaa gattatcttg aacaattatc
    39481 tgggaaattg gtcaaacaat atgactactt atttgtagaa tcttttccaa aagaagaggc
    39541 acatgctgat ttttctattc acgactggca taaattaata acaaaattgc gttataagtc
    39601 ccaatggtat aataaaaaat ttcttttgat caataccgac ggagcagaag aaagcaattc
    39661 cgtaagaaag agccaagtag tagaaaaaat gggcagacat tcggttatca agggataaaa
    39721 aatgaatgaa actgatagga gcgttagggt tagccttgtt aataaaaagt cttaatgact
    39781 tttgtttcta ggaatttttc ttttgaagaa aagctcacta attatgacaa accaataaaa
    39841 caatagcaga aaaagttcgt acgagctttt tctgctattg ttaatttttt attcagaaag
    39901 tttacgaata catcaaaatt ctgaaacaaa aaacgtaact cacaaagagt tgtttttgcg
    39961 ttttttcttg aatgattagg tctgaatctt ttgctcacaa ccttattcat ggtgccccgg
    40021 cttgagctga tagatttttg aaggacggcc gattcctaca ggacgttcac cgatctcatc
    40081 gaaatattgg agcatagctt tcttaaaatt ggaatgatcg atcgttttat aatcgatccc
    40141 taagaattta gcaaatactt ttcgagcttc cgtaatcgtg aaatctttac ctaaaacttg
    40201 tagaacttgc ggctcgtgtt ccattttatt gactacacgg ttgaatgctt tcaagatgat
    40261 ttgactatgg tcaaaagcca aagtatcttt gccagaagac tctccagtga ctaaatcaag
    40321 cgagatagcc acttctccgc tagataaatg gatcttgtta ctatgacgtt ctagtgtgaa
    40381 ccagcgtact tccttggcat catctccggc gattagcggt tcttctccaa taaaggctaa
    40441 gtagctgact gtgacgaccc agcctcgcgg atctcgatta ggcgtactga atgtatgcag
    40501 ttgctcaata ttttcttttg aaataaccac accagtttct tcctttgttt ctcttaagac
    40561 gctttctcca gttgactcat ccttttggac aaagccacca ggcaatgccc atgaatttcg
    40621 ataaggatgc ccttttcttt ggatcaacaa aactttcagc tgatcttctt ctttgttata
    40681 gcacatcagt acgatatcga cagtcaaaga gggcttgtca tattctggac gttcctgttt
    40741 ttgataccag ttaagaaatt cttcttcact tgctttatgt tcataatata atttttcttc
    40801 ttctttagaa gcaaaacggt tcatctgacc acctccattg acagtaaaag tactttttac
    40861 cttattgtag cagactctga aaaaaaacca aggatttact tgaaaaatct aggttatcat
    40921 ttaaatatga aaaataaatg gaagtatgag attgaataaa aagaaaagca gataaaacgg
    40981 caatacttta taccgctctt cacgaatggc aaagaagcca caaataacga tatatacgtc
    41041 aatgataaaa cgcaaaatcc aagctgctgt cataaaactg ttcaaatgcc catggaggtt
    41101 aaaataacca tacatacgaa tataaaaata gctgaatatt aaatgtgcta atgaaagaaa
    41161 agcaatacta agtgtaaaaa aaagatgttt cttctgcatg attgttccag tcccttacta
    41221 agatagatag tctttattat accacgtgtg aggggagaaa taaaaagccg ttttttagta
    41281 gatatgaaac aaaacaaaaa cccgcattta ttcaacctat tcatcctttt aaaggataga
    41341 ttgaacgtag atagaagtcg tttatccagt ctttttcatt ttgaaaaaaa gctaacagca
    41401 tgcttttttt atggtatact agcaaggaaa cgaattcgca tcatgcgata aaggaggaag
    41461 agccccgtgg cagaaaagga aactttttat atcactacgc caatctatta tccaagcggc
    41521 aaacttcata tcggcaactc gtatactacg attgcatgtg acgcaattgc tcgttataaa
    41581 cggttgatgg gctttgatgt gttttatctc actggagtgg atgaacatgg tcaaaaaatc
    41641 gaaacaaaag cagaagaact aggtgtaaaa ccgcaagaat atgtagataa aatggcggct
    41701 gatgtcaaga aattatggaa aacgcttgat atcagttatg ataaattcat tcgcacgaca
    41761 gatgattacc ataaagcagc tgttcaaaaa atatttgatc gtttattaga acagggagat
    41821 atttacttag gtgaatatga aggctggtac tctgtttctg acgaagaatt ctttacagaa
    41881 acacaacttg cagaagtgta caaagatgac gaaggtaacg tgatcggtgg taaagcgcca
    41941 agtggacacg aagtagaatt ggtaaaagaa gaatcatact tcttccgcat gagcaaatac
    42001 gcagaccgtc tattggaata ctatgaagaa catcccgaat tcatccagcc agaatctcgt
    42061 aaaaacgaaa tgatcaacaa ctttatcaaa ccaggattag aggatcttgc agtttcacga
    42121 acaactttta cttggggagt accagtcaag agtgatccga aacatgtggt gtatgtatgg
    42181 atcgatgcat tatccaatta tattactgca ctaggatatg gctccgaaga tgaaagtcta
    42241 ttcgaaaaat actggcctgc agatgttcag atggtcggta aagaaatcgt tcgctttcat
    42301 acgatctatt ggccaatcat gctgatggcc cttgatttgc ctctacctaa aaaagtattt
    42361 gggcatggct ggctattgat gaaagacgga aaaatgtcta aatcaaaagg aaatgtcgtt
    42421 tatcctgaaa tgcttgtgga acgttatggt ttagatgcac tccgttatta tttgcttcgt
    42481 gctgttccgt ttgggtcaga tggtgtcttt acgccagaag attttgtttc ccgtgtcaat
    42541 tatgatctgg caaatgatct agggaactta ttgaatcgaa cgattgctat gatcaacaaa
    42601 tattgtgatg gactcgttcc tgactatgcc tcacttgtca ctccatttga tagtgaactg
    42661 tctacgacgg ctgctaacgt tgtcggacgt taccatgatg caatggaaaa aatggaattc
    42721 aatacagcat tggctgaaat ctgggtgctg atttctcgtg caaacaaata cattgatgaa
    42781 acagaaccat gggtattagc aaaagacgaa gaaaagaaag ctgaattgaa tagtgtcatg
    42841 atccatttag ctgaatcttt gcgtatcgca gcgatattac ttcagccaat catgacagaa
    42901 acgccagaga agatcttcaa tcaactagga ttggactctg aaacaatgaa tcttgaagga
    42961 ttacactttg gcgaatttcc ttcaggaaca aaagtagttg caaaaggtac accaatcttc
    43021 ccacgtttag atatggaaga agaagttgct tttattcaag aaaaaatgtc tgaaggtact
    43081 caaacaaacg aagatacagt caaatgggat ccagaagaaa cagaacttgt ttcaacgaaa
    43141 gaaaaacaaa tcaaatttga tgtatttgaa aaagtagaac taaaagtagc agaagtcatc
    43201 aactgtcaaa aagtagaagg ggcagataag ttgctgcaat tccgactaga tgctggagac
    43261 agccaagacc gtcaaatctt gtcaggaatc gctgaatttt atcctgatcc aagcgaattg
    43321 atcgggaaaa aagtcgtcat tgtagcaaat ctgaaaccac gaaagatgcg tggacaaatc
    43381 agtcaaggaa tgatcctttc tgcagaagca ccagatggct ctttacaagt aatcgaagca
    43441 ccaaaatcca tgccaaatgg ctcagaaatc gcatagaaat tcaaaggggg tatgacacta
    43501 gttgtgtcat accccctttt tccgaaattt gagaagcaat tttcgtaaat tccttcttat
    43561 tacttgaagc caatcatata atacagaaac agagaactat ccaaaataaa tcctagaaaa
    43621 agaagtacga gcttcgctga tttttgttaa tttagaataa tactaatagg aatcgtaaaa
    43681 acagaaaaaa agcataatga taaagggtcg ggcagatttt aattgattca tctggtactc
    43741 ttttttattg tccttacatt cataaagttt gaatgcaatg actttattac atctatattt
    43801 atatctaaca gtagcgttga tgtgttaatt ataagttcta tcactttatg ttcaattagt
    43861 ttgtgctctt atatgtgtga ttgaaaatat agataaaaaa acaatcatcg gctttgactt
    43921 cgatgattgt tttctttatt ttgtaaaaat cctatcactt gattttatgg taacctttta
    43981 tttttgtctt caaacggctt ggtaagagag cagtaagcag tgcaaatggg atcgaaaaga
    44041 cataccagaa aatcgtacgt ccgataccat aatgttgata gagctttgtc aacatcacgt
    44101 gtaacaagat caaccctaaa agcaaggagc cagctataaa ccaatgatta tcccaatgca
    44161 tgacttcact aagtgccatc actccaaaag gatagaaata aaaatagatc ccgactaagg
    44221 tcatccgcca gttctttgta aatgttccga tattcaataa aaagaaaatc gctgaaagca
    44281 aaataccgat aggcggaaaa agcccaacca aaaaggaata caacatcccc cacaagatca
    44341 tcacaggacc tttgatcaat gcaacgattg cgatgaacaa agtcagcaat attagttttt
    44401 gtgataacgt tgcaacagtc attaatccaa ataaaattaa tgtgaaccaa tgccaatggg
    44461 actgctgttg ttctttacgg gaaaaatcga cccggttgga taaataatct gctaatttcg
    44521 gataagaacg cttaaaatcc ataaactgtt cctccttcgt ggaaaaggta ggtttagtat
    44581 acgaaaaata aaaaagaaat acatccgtct taagagggag tttttcactg aaagtggaga
    44641 aaaaagaatg gtaaattaaa atattctcag ttatactgaa aattatacca aatttcaaag
    44701 aagaaagagg atgggaatga aaggcaaact aaaacgagaa atcaatctgt ttggcgcatt
    44761 agctaccgtt atgggaaccg tgatcggtgc aggcgtcttc ttcaaaactg ctgctgttac
    44821 tgctagtact cagtctacaa gtttgacatt gctagcttgg cttttgggtg gtttcttaac
    44881 gatctgtgct ggattgacag tggctgaatt agctacagca atcccggaaa ctggtggagc
    44941 tgtaaaatat attgaagctg cgtatggaaa attgcccagc tttttattag gatgggcgca
    45001 aagcttgatt tactttcctg ctaatattgc tgcattatcg attatctttg cgacacaatt
    45061 gacgaactta ttacaattat ctaccgatta tcttttgttg attgcagtga tcaccgctgt
    45121 ttctgtgaca gggttgaatt tgttagggac aaaagtcggt gcttctgtac aatcagtgac
    45181 gttgattgtt aaattaatac caattgctgt gattgtcata tggggattat tgaccccagg
    45241 acaaggaact gtccagttat ttccaatcga agctggaaaa gatgttactt ttgtcgaagg
    45301 attgagcagt gcgttgttag caacgttgtt tgcttatgac ggctggttag gtgtcggtgc
    45361 aatggcaggg gaaatgaaac ggccggaaaa agatttgcct aaagcaatta ttcttggttt
    45421 gagttttgta acagtcgttt atctattgat caattttgtt tttttgaaaa ctttgccgat
    45481 tgatcatctt gccggtaatt tgaatgcagc ttctgaagca tcagacgtca tttttggagg
    45541 aatcggtgga aagctggtca ctattgggat cttgatctcg gtatacggtg cattgaacgg
    45601 ttatacacta actgggatcc gcgtgcctta tgcgatggct ttagaagacg agctaccttt
    45661 cagtaaacag ctgacaaagc tttcaaagaa gtttacggtt ccttatgttc ctgcggtttt
    45721 ccagttagca gtagcttgta ttatgatgag ccttggttct ttcgactttt taacagacat
    45781 gttgattttt gtgatgtggc tattcagttt actgattttt atcggtgtct ttattttaag
    45841 aaaaaaagcg ccggaattgc cacgtccata taaagtacca ctttatccaa tcgttccgat
    45901 tattgcgatt ctcggtgcta ttttcatttt aggtatgacg atgatgacgc aaacgaaatt
    45961 agcattgatc ggtataggtg ttaccttgat aggtatccca gtctattatc aaaaaaagaa
    46021 aaaacgctca gaagagtaaa atatttcaac aacagacaga aaaagtacga actaggtcct
    46081 ctagatcttc gtgctttttc tatttcaata aaacaagttc ttctattttg atttttgcta
    46141 cttctgcaag tagtgcttat gtgctaaagt aagaatgaaa aggagagata aacatgatat
    46201 ttgattctca tacccattta aatgctgaac agttcaatga agatattcca gaaacgattc
    46261 agcgagcaca agaaatgggg gtaacgaaaa tggcggtcgt gggcttcgat acgccaacga
    46321 ttgaaaagtc tttgcaatta agccatgact atccaaatat atacagtatt atcggctggc
    46381 atccgacaga ggctggcagt tatacgaaag aaatcgaaaa taaattacaa gaacaattga
    46441 ctttaccaaa ggttgtagca ttaggggaaa ttggcttgga ttatcattgg atggaagacc
    46501 cgaaagaagt ccaagataaa gtatttcgca gacagattgc tatcgcaaaa gaaatgaacc
    46561 taccaatcag tattcatact agagaagcac ttgaagacac gtatcaaatc ctaaaagaag
    46621 aagatgttcg agatatcggt ggaatcatgc atagtttcag tggtgattat gaatgggcaa
    46681 aacgatttct tgacttaggg atgcatattt catttagcgg tgtcgtcact ttcaaaaaag
    46741 cattagacgt ccaagaggct gcagcaaatg taccaatcga acgattactt gtggaaacag
    46801 atgcccctta tctggcacca gttcctttta gaggaaagcg aaatgaacca ggctatactc
    46861 gttatacagt agaaaagatt gctgaacttc ggaatcttcc gacagaagaa gtggctgccc
    46921 aaacatggac aaatgcgcac cgattatttg gtctaagcga acatggataa gatgaagatc
    46981 caagagatta tagtcgttga aggaaaagac gatacaagaa ggctgcggga agtagttgat
    47041 gcagatacga tagaaacaat agggtctgca atcaatgaag agatcctgat gcaaattgaa
    47101 catgcccaag agactcgtgg agtgatcgtt tttaccgatc ctgatttttc aggagaaaaa
    47161 ataagaaaaa tcattatgga agctgttcct caggcaaaac atgctttttt gccgcgcaca
    47221 caagctaaac caaagaaaaa gggaagcctt ggtgtcgagc atgcaagtga tgctgctatt
    47281 ttagaagcat tgaaaaaggt cgtgacgcca gacatttccc aaacggctgt ttctgaaatc
    47341 actagacaag accttttaga ctatggttta atagctgggg cacaagcaaa agaaaaacga
    47401 gaaaaactag gtgacgagtt gcgcatcggt tacacgaacg gcaaacaatt ggaaaaacgt
    47461 ttgaagatgt ttcgtattac gccgaaagaa ctcgcccaag caatgaaaat agtggaggaa
    47521 caacatgagt gaatatcgtg aaattgcgac accctctaga acaaaagaga ttttattgaa
    47581 acatggtttt tcatttaaaa aaagtttagg acaaaatttt ctgactgaac caaatatttt
    47641 aaggaaaatc gtagaaactg cgcaaataga tgaacatacg aacgtgatcg aagttggacc
    47701 aggaatcggt gcattgacgg aacaattagc caagcatgca aaacaagtcg tagcatttga
    47761 gatcgatgat cgtttgatcc ctgtattggc tgatacgatg caaccctatg acaatgtgac
    47821 tattgtacat caagatattt taaagaccga tttgtcaaca gctgttagag aaacatttca
    47881 tgaggagctt ccgttaaaag tcgtagcgaa tttaccttac tatattacga caccgatcat
    47941 gatgcatttt cttgaatcag atttggttgt ggatgaacta gtcgtcatga tgcaaaaaga
    48001 agtagctgat cgtatctctg ctgaaccagg aacgaaagct tatggttcat tatcgattgc
    48061 tgttcaatat tatatggaag ccagtttagc attcatcgta ccaaaaacag tatttgtccc
    48121 tcagccaaat gtagattcag cgattttgaa actgacaaga agagatatac cggcagtcga
    48181 agtgacagac gaaaaagcat ttttccgatt gacaaaagcg gcatttcagc aaagaagaaa
    48241 aacgctttgg aataacttgc agcattcgta tggcaaagat gaccagacaa aagcatggtt
    48301 agcaaaaagc ttggaaacag ctggaatcga tccaaaacgt cgtggggaga ctttgtcatt
    48361 acaagaattc gccgcattaa gcaatgcgat gtcagaaaat atgcagtaaa aaaagaggtt
    48421 gtgacagaag ttgtgaaaaa gctgtcctgc atcaagtaat aagaacagcc aaacaaaaat
    48481 agttcctcat gtttttcgtt tgtttgggct tgttaccgca gatgcaagct tttgaacacc
    48541 gtttaagaaa gaggttgtgg gcctgccgtt taactctaag tcacaaggaa caatttcaaa
    48601 aaacagcttc tcatgttttt gagttagttg gtcttattac ttgatgcaag cttttgacca
    48661 ctattatgcc gaacaaggag atgtgacaca aattttgtca catctcctat ttttgtttct
    48721 tgatcaaaaa attcttatac ttgatactca cactcatttt gtatggattt agaaaagaat
    48781 atgtgtcttg acagttatta gtaagtgagt tatactaagt cgacatttat gtgtcaagac
    48841 atattaggag agagaaaaaa tgagaaaaaa ttcagttcga aaactaacga tttctgcttt
    48901 attgattggg atgggggtca ttatcccaat ggtcatgcct aaaatcatga ttccaccagc
    48961 ttcctttact ttggcgtctc atgtgccatt atttattgcg atgtttttta caccaggagt
    49021 tgcagtggct gttgcgctag gtacgacatt tggtttcttt ttaacaacgc cggttatcat
    49081 cgcattgcga gcattgtcac atcttgtgtt tgcgttgatt ggggcttggt acttgcaaaa
    49141 gcatccgagt atcgttttaa aaaatggaca gtttactttt gctaattggc gtttccaagg
    49201 gttcaatttt gtgatcggtg tgattcattc tttagcagaa atgctagtag tcagcatctt
    49261 ttactttatt ggtaatatgc ctgaaacata ttattctcaa ggatattttt atacagtatt
    49321 catcttgatg gggattggtg ggcttatcca cagtttagtt gattataata ttgcttattt
    49381 tatagcagga acactcagca aatattttga tatccccgta tttacggcag caaaaaaaga
    49441 aaatcggaaa gagtcgattc aagggtacgc gaagaaaacg acatattaga catgatctct
    49501 caaaaaaaaa actctcatct agtaatggat aataaatcct ctaagatgag agttttttgt
    49561 cctaaaaaga gctgcaccaa catcttctgg cacagctcta acagggggca atttatggaa
    49621 gtttgatgac ttgttctgtt tctttatcaa agaagtggcc tttgctgatg ttgaaggcaa
    49681 ggtcgactat ttcgcctggt ttatggaaat ctcttgcatc tacttttgag atgaactcag
    49741 tagaaccagt ttttgtgtaa agcatcgttt cagctccaag taattcggaa acgacaactt
    49801 ctgatttcac tacggcttca ggtgaagctt caagggctac ttgttcactg tggatgtctt
    49861 ctggacgaat accgaagata acttcttttc cttcataacc tttttcaact aatactttgt
    49921 tttttccttc aggaatacga agcttaagcc catgtccatc tgtgataaca ccatttttca
    49981 atgtcacatt aaagaagttc atggcaggag atccaataaa gccggcaacg aacatattga
    50041 cgggtgtatc ataaacttct ttcggtgtac cgatttgttg gataaatccg tctttcatga
    50101 taacgattcg gtcagccata gtcattgctt ctgtctgatc gtgagtaaca tagatggtcg
    50161 ttgtttcaag acgctggtga agtttggcaa tctcagcacg catggctaca cgtaatttag
    50221 catctaaatt tgataatggc tcatccatta agaagacttt tgcatcccga acgattgccc
    50281 gtcccaatgc cacacgttgg cgttgtccac cagataaagc agctggtttt cgtttcaagt
    50341 attctgtcag acctaagatc tctgctgcat tgtccacacg ttttttgatg tctgctttat
    50401 catatttacg taatttcaag ccaaaagcca tattatcaaa aacagtcata tgaggataca
    50461 atgcgtagtt ttggaaaacc atggcaatat cacggtcttt aggtgccacg tcattcatga
    50521 cttttccgcc gatatttagt tctccttcag agatatcttc cagtcccgcg atcattcgca
    50581 aagtagttga ctttccacaa ccggaaggac caacaaaaac gataaattca cggtctttga
    50641 ttgatagatt aaaatcagtt acagaataat tttctgcatt gtcatatttt ttatatacgt
    50701 ttttcagagc catttctacc ataatacgtt ccacactttc ttttttagtt ttattaacta
    50761 tttaaagtat aaatgaaagc gttaactaac ttcaatgtca tgttgaacaa gaattttttc
    50821 ttttttttgt caatgtgcta gttttaagct ttaataaata tccataagaa ataaaaactg
    50881 atacactgga gtaaataagt atgttaacaa tatcaaaagg agtgtctctt atgaaaatcg
    50941 ctttgatcgc tcatgaccga aaaaaaccta tgatggtgaa attagcaaca gcgtataaaa
    51001 caatattgga aaaacatgaa ctttttgcta ccgggacgac tgggcagagg atcacggaag
    51061 caaccggctt gccgatccat cggtttaaat ccggaccgtt aggcggggat cagcaaattg
    51121 gagccatgat ttcggaggat cagttagatt tggtgatttt tcttcgagac ccgctggctg
    51181 cacagcccca tgaaccagat gtaacagcac ttatccgttt atctgatgtc tatgagattc
    51241 cgttagctac gaatatcgga acagctgagg tgctactaag aggactggaa gccggcttcc
    51301 tagattttcg aagaatcgtc catgaaatag acaagcatcc actttcattt tgatggataa
    51361 gcgtatggaa aaataacgtt taatgttcgc ctatatgtag ctataaaagc taaacaaact
    51421 gataatatgc gatataatac gaacgtattg gataatataa acaataatat tagtttttcg
    51481 tatttgcagt aaatgtgaaa atatgctata gtttctgtgg gaaaagatcc caaaaataat
    51541 acagagtaga aaataaagcg ttaagtgcta agaggatggg atgttgcctc ttggacgaag
    51601 gacgtaatgt tcgcggtttt attttcgcat tcgctgcatg atacgatgta gcagctctta
    51661 aaggagatgc taaaaatgaa gaaacaccgg ttagacgcac gtatgattgc tattatgggc
    51721 ttattgattg cattgatggt cactttgtcc cgactggtag caatcgagac cccatttatt
    51781 aaaatcagtg tcacatttat tccacaagtg atcatgggca ttctttttgg tcctttttgg
    51841 tcagggatag gcgcagtttt agctgattta gtaggaatgg ccctcttctc gaaatctgca
    51901 ttctttattg gatttacatt gaatgcattt attgaaggag caatctacgg attctttttc
    51961 tatcgaaaag agatcacttg gaaaaatgcc attctggcta cgcttagtgt cacgctgatc
    52021 atcaacttat ttttgacacc attatggctt gcgttgatgt accatgtgcc attgttcagc
    52081 tgggtcgttt gggcgccccg cctgttaaaa acagtaatat ggttaccgat tcaatcaatt
    52141 gccatttatt acgtaggacg tagtattcct tacaaaaaaa ttttaagaag tttagcgatc
    52201 cacgctaaat aaaaaacaag cagtcttccc atcccagaag actgcttgtt tttattgtgc
    52261 tgtgaattgt gcttgataga gccgtcggta ataaccatct ttttgaagca gctcatcatg
    52321 ggtgcccatt tcgacaatag ctccctgatc catcactaat atctggtccg catttttgat
    52381 tgtggacagg cgatgggcaa tcacaaaact ggtttttcct tccatcattg ctaagaaggc
    52441 ttgttggatt tttttctctg tcaatgtatc gactgagctg gtagcttcat ctaagatcaa
    52501 cataggggga ttactgatca tcgttcgagc aatcgtcaac agttggcgtt gtccatctga
    52561 tatctttaac ccttgaccgc caatcttagt atctaatttt tccggcaaac gttctacaaa
    52621 ttcatacata taagaggctt ttagggcatc attgatttct gcgtcacttg cttccggatt
    52681 cccaaaacga agattttctc gcaatgtact gtcaaatagc caagtatctt gtaacaccat
    52741 tccaaaactt ttacgtaatg tatcacgttg gatagagcgg atgtcatggt catcgactgt
    52801 aattgttcct tttgttgtgt cataaaaacg catcagcaaa ttaacaagtg tggattttcc
    52861 ggcccctgtt tttcctacta tagcaatggt ttgacctgga taagcagtaa aattgaaatc
    52921 ttcgatcaaa cgctgcttgg ggtcataaga aaaagaaaca tgatcaaaag agacttttcc
    52981 tttgatattt ttgggttcca aggcataggc atctggcaaa tcaggctttt cttctggctg
    53041 atcgatcaaa tcaaatgctc gttctagacc agaaaaagcg gtttgcagtt gtgtcgtgat
    53101 tccagaaagt tcgataaatg gtttagaaaa ctgacttgag tagatcgtaa agctggaaat
    53161 cacaccaaca gtgattgacg aaccactctt caatgccaat aggccgccaa ctaaaccaat
    53221 cgataaataa gcaaggtgat ctacaaaacg agaagacggg tttgtcaagg aagaagaaaa
    53281 ttgtgcccgt tgtccttttt catatagatc agcatttagc agttcaaatt gctgttgact
    53341 agcttcttct tgctgaaaag ctttcacgat tttctggtta ccgattcgtt cggtcacaaa
    53401 gcccgagata tccccaataa tcctttgctg tctatcgaaa tttgtttgtg aagcacgagc
    53461 aacgatccaa ctcacaagga aaataattgg tgtagaacaa agaacgacca atgtcagaag
    53521 tggacttaga cgcatcataa aaataaacga aagaatgatg attgccactc cagaaaataa
    53581 ttgattgaag gccgcagaaa ttgcaatgga aatattgtcc atatcatttg taaatcgact
    53641 gacgatattt ccgtgggagt tttgatcata ataacgaagt ggcaatcgat tcagatgagc
    53701 aaatgcatct tttcttaatt ttgcaacgga taagtaagct aaccgattgc ttaacacttg
    53761 gatgagccat tgactgacta cgttgattaa aacgatacca ccgaatagaa taagtaaatg
    53821 gaacagtcga ttgaagcgga cctgtccttc tccaatcatt tgatcgatac tttgaccaat
    53881 ttgtaacgtc atcacaactg ttgcaatccc attgatgatt cctagtatca cagctagcca
    53941 aatgtctttt gcataggcag taaggtaagg aatgaatcgg cggagcgtct gcaaggataa
    54001 tttttttact ttcataacta ttcttcctcc tgtgatgcga cgatttcctg atattctgct
    54061 gattgagcaa gtaattcttc atgggtaccc tgagcagcaa tcttaccttg atccatcact
    54121 aagatatgat gtgcttcttg aatagagcgg acacgttggg agataataat cactgtactg
    54181 ccaagataag tctgtagcgc cttacgcaaa tccaagtctg tttgataatc caacgcactt
    54241 aaagagtcat ctaaaatcag taatgctggc ttggcaatca aagctcttgc aatcgtcagt
    54301 cgctgttttt gtccgccaga gaaatttttc ccattttcca tgacttttgt atctaaacca
    54361 tttggaagct gacggacaaa ttcttctgcc tgagcgattt tcagagcttc ccaacattct
    54421 ttttctgtgg cgtctttctt accccattga aggttttcac gaatagtccc ggtaaatagt
    54481 acagaagttt ggggaacaag agcaatttgc tgacggagat gatccagctg ccaagaacga
    54541 acatccattc cgtccattat aactgtaccg gaactgacat catagaaacg cggaatcaat
    54601 tgtgtcaaag tcgttttccc gcttccggtt ggaccaatga ttccaaggac tgtatttcgc
    54661 ttcagctgaa atgtaatatt ttggagagaa agaccagaat caggagtata acggaagtca
    54721 acatggtcaa aagcgataat tgcttcagaa gaaaaatcgg gagtagtact gacctcagct
    54781 tcaagaatcg aattttctgt gtctagcacc tctttcaccc gatttcctga agcttcggca
    54841 cgtgtgaaga tcacgacgag gtttgaaaca acgatcagtg ccaaaagcat ttgattcata
    54901 taattgatca aagcaagaac ttgtccttgt tgcaagctac caatatttac tttgatcccg
    54961 ccaactgtca gcaaacagat gatcccgaca ttcatgatca aggttgtagc aggtgataat
    55021 agggcagaga tattggaaac acgtaaataa ttgttcgcta gatcatttgt gcttttatcg
    55081 aacttctctg tttctttttt tgtctgcgca aatgcgcgga tgacacgaac accgctaaga
    55141 ttctgactaa cgtgttcatt gagctgatct agtttttctt gcactttttg gtagagagga
    55201 acggtttttt tgatgataaa aaataaaatg attgagaaaa taggcaataa tgctaagaaa
    55261 aacagaccta cttcccagtc gatcacaaaa gccatgacca ctgacccgat acttaaaaac
    55321 ggtgctctga tcaataaacg aatgaccatt gctaaagcta gctgaagctg attgatgtcg
    55381 tttgtcattc gggtgatcaa ggtatctgtt ccaaaatgat tcaactcgtt gtgagagaaa
    55441 gtattgattt ttttgattag ctgattacgt aactctgtac caaatccttg tgaagctact
    55501 gaagcatagt attggcaaat gatagcacaa atcagaccaa tcacagacat agcgaccatc
    55561 cataagccca tttcaatgat ttttcctcgg tcattcattt ttagtccatt atcgatcaag
    55621 gaagccatca ttaaaggcag gacaagttcg aagacagctt caagaaattt gaagaacggg
    55681 cccaaaatga tttgcttacg atagtctttt gcataacgta ataactgaat catatgattc
    55741 ctcctattat ttatttgtcc acaattgaaa aaacaagtac taccacattg tagcttagga
    55801 aatttagaat gaaaagagaa caagcctttt cataaatgaa agttttatta tgaagaagct
    55861 ttagaaattg aatagactat tttttttaaa aaaaagaaaa tgagaaaaca tgccagtctc
    55921 ttcattcata ggaacgtcta ttttacttaa tttttctata aatgaattct gcataaaaaa
    55981 tatatcaagt aaatgtttat agatgaaact ctatttactt cttttttaat ttgttcttgc
    56041 aatctttttt gctacgtaat ttttgtaaaa aagtgggttg agagaaatct aattgatttt
    56101 caaagaagga gtatgaaaat gagaaaaata tttattcgtt tattgtgggt actatcttag
    56161 aggaataaat aatagtggag aaaactatgg tttggcgcag attctatcag gtttgatgga
    56221 acgaagacaa cctcccgaca tcaagtcaaa agctacttga gattttgatc tgaaaataaa
    56281 aaaagaaaac aaggtaggaa catcaatgtt tctaccttgt tttcgtaatc aatgggaaaa
    56341 aactaagagt ggtgtacgta atcaataaaa aggatggtga caacagtacc tctttactct
    56401 ctgaaagcat cataactcgt ttttcctcat tatgccaata aattgttgtt ataccacata
    56461 taaggatttg taatagctaa aaacagacag gaaagttgaa atctgtgaat cttattttgt
    56521 atggaaggat ttatagaacg caaaaatcaa gataaagatg aataaaaatc ctgtttcggc
    56581 tatatattgc gtatctacgc caataaaacg agtttgttct gtcatagcca tataaagcaa
    56641 aggcaaaaat acaactacag cgagataaga tccgatgatt tctcgaaaag ttttccagga
    56701 agtatgtttc ctgtttttat atttatccga tttcattgaa tagtcatagc ctccttattc
    56761 tttcatatgt tgaaattcca gtataaaaac tggaaggaac tgtacgtttt ttagaataaa
    56821 aattataagg gataagtagg ctgctgtcta taataagata tatttttcaa ttctttatta
    56881 aagattttat tgacaaaatg aatccgtttt ctatgatgga ggaggggtga ataagttgtt
    56941 gaagaaggaa attttatgtt acttagagaa ccagacagcc tttttcgatt tagaacatat
    57001 gaacgaggtc tttacagcac aaaggttagc tgatcatttt ggggtaaaaa gaaacacagt
    57061 cagtcattat ttgaaccagt taaatgatga gcaactacta gtcaaaatca attctcgacc
    57121 agtgatttat ttccacaaag aagcttttga aaaacaattt tttaagttac gcacgaattt
    57181 ttatttttct atcgatgatc tcaaagagga acagcctttt ttcgcgcaac caaaagactt
    57241 attctctttt atgatcggat acgacgccag tttgaaagaa agtatcgaac aaatcaaaac
    57301 agctctttac tatccagatg gcggattacc acttttgatt acaggcgaaa gtggcacagg
    57361 taaaagttat ttagttaatt tggtctatca gtattgtctg cttcatgatc tgctagaaga
    57421 ttctgcacca ttcatcactt ttaattgtgc acaatatgca gataatcctg aacttttgac
    57481 tagcaatttg tttggacatg ttaagggagc gtacactggt gcagaagaaa ataaaaaagg
    57541 ggcttttgaa gcagcagatg gtgggatctt gtttttagac gaggtacatc gcttaacacc
    57601 agaaggtcag gaaaaactgt tcacctatct tgatcaaggt gtgatttatc gtatgggaga
    57661 acctaatttg tcacgccgga tcaaaacacg tctctttttt gctacgactg aagagattac
    57721 tagtcatttc ttaacaactt tcattcgaag aatacctatc cgaatcgaac tacctgcact
    57781 taatcagcgt agtcgtaatg aacggctaga actgatttac tctttttttc tcgaagaaca
    57841 aagaaaaatg gggagccctt tgtcagtcag tggaccagct ttgtcacttt taacaagtca
    57901 gaaattatcc ggaaacatag gagagctgaa aaatatcgtc aaagtttcag cagcaaaagc
    57961 atatgctgaa cagcgtgaac agcctgaaat ccatgtcacg atccatcatt tgcaaaaaga
    58021 actacttgcg cagcctatta ctaaaaatac agcacagcaa ggaatctata ttacgcaaga
    58081 ccagacctta gagcaattaa tggagaagca acagcccgaa cagcagcgaa tcgttaaaag
    58141 ttttgagcaa atcctaattg atttcaagaa aaataactgt ctgctatatt cttgcgaagg
    58201 aaaattaaag caagaagtga ttcaattatt tgattttttg ctgtttgaga ccagtagaca
    58261 agaaaaacac gagctgctgg tttattggac acaatctatc cgagagacct ttcgacaaat
    58321 ggaaactgcc tatcaaatca aattcgatgg aaacagtgtc tatgccttga gttactatct
    58381 ctatcagcga agcactatca aatggttacc ggaagaacat gatattatcc aactaatcaa
    58441 agaattagaa caacaagttt ctcagtctta tccatctagt tatcactatg tccaacgaat
    58501 tctggaatta agtaagatca aattagatat tgaagacaca agtatggatc ggattttact
    58561 gacgatctat ctaaaaaaag cgaaatggtc aaaagaaaaa cgactgccaa aagcgatgat
    58621 tgctgctcac ggttatgcaa ctgctagcag tatggcaaat gtagtaaatc gtttattaca
    58681 agaggatcta tttgaatcat ttgatatgcc actagatgtc acaccacagc agattgcttc
    58741 agaaatcctt gattattgcg aaaacagcga tgtttcaaat ggattgatca tattagtaga
    58801 catgggatct ctgaaagaaa tccatcagtt ttttaaaaaa cagttatccg taccgctttt
    58861 gattttgaat aatgtgacga cacctttagc aatcacagta ggggaatgcc tgcaaaaaaa
    58921 tgtatcatta gaagagatag cagaagagac tgttcgacag atccagccag aatggcgact
    58981 gctctatcca gaggaaaaca aacccaaagc actcattact acttgcttca ctggaatcgg
    59041 tacggcggtc cacttaagtg aactgctaga aaaaagctta cctacggctt gtcagttgaa
    59101 aatcatccca tatgaatatc agcaattaaa agataagaaa aacagtgacc cattattttc
    59161 catatacgaa gtcgtaggaa tgattggcac cacagatcct gatatcacca atatcgacta
    59221 tctttcatta gaagacctca tctcggggga aaagatgtct gtactagctg aatggttgga
    59281 cactactatg aatgcttcag agaaggaaat attcaatcag cgcatcattc gtaatttttc
    59341 attggaaaaa gtactggatt ccgtgactat tctagataca gagaaggtga tgggagaaat
    59401 cgatcatttt atgagagaat tagaggtgca gttgaatcga agcatcagca atcccaaaaa
    59461 attagcgttg tatgtccatg tgagctgtct cattgaacga ttgatccgac aaatgccaat
    59521 cgaaacctat caaggattag agaaatggaa gcagtgtcaa aaagaagggt tatctgctat
    59581 taaatctgcg tttagtgtca ttgaagagca ttatagtgtc atgatcccca attctgagat
    59641 tgcctatatc tatgatattt tgagtgaaga tacagagttt ctctcgatag aagaagaatt
    59701 ttgaacataa gcaagttgtt gccactaatt ttggcatgca acttgctttt ttagtatgtg
    59761 taagtgaaaa gaggagatga aaaagtggat agagaaatta tcttagcttc ccatggaaaa
    59821 tttgcttcag ggatattaga ttcattagag ctgatctatg ggaaaaatca ttctattact
    59881 gtattcgatt gctatactga tgagaatttt gatttagcgg aaacagtaag gcagcttttt
    59941 atgaggtaca aagataaaga aatcattgtt ctgacggatc tttttggcgg aagtgtcaac
    60001 aacgaattct tacaatatat caatcgggat catgtctatc tcgttgccgg attgaatttg
    60061 ccactattga ttgagctagt atctcggttg gacgctgaag tagagacaac tgttttgatc
    60121 cagcaggtgc taactgattc aaaaaaaatg atccagtttt gtaacgagcg gctcaatcag
    60181 aaaatagaag aagacgaatt ttaggaggaa gaaaaatgat tgtattgaca agagtcgacc
    60241 atcggttatt acacggacaa gtagcttttt catggacaca aacaataggc gcagactgta
    60301 ttttgattgc taatgatgat gtacctacga atgagatacg aaagacaacg atcaaattag
    60361 ccaagccaca aggagtcaaa ctagtgatca aatcaattga tgactcgatt gccgcgttga
    60421 aaagcggggt gacagataaa tataagttat tcatcgtagt ggaatcgatc gaggatgcat
    60481 ataagctggc agaagcgtat ccagaaatca aaaccatcaa cttaggaggg atcaaggcaa
    60541 aacctggtac tcgcagcatc ggaaaagcgg taaatatttt accagaagag gaggaacgct
    60601 tgaaacgatt aattgacaaa caaatagaag tagaaatccg tcaagtacca gcagataaaa
    60661 aagtggatgt aagagaagtt ttgtaagaaa ggaagaacct caatgattgt acaagcagtc
    60721 ttattaggaa ttgttgcatt tgtcgcacaa tcagaatatg cactagggac atcgttgctt
    60781 tctcgtccga ttgtgacggg gctgtttaca ggaatcgtgt taggtgatgt aaaaactggg
    60841 atcattatgg gcgcaacttt agagttagca tttatcggat cgttctcagt aggtgcttcg
    60901 attccacctg atgttgtgac cggtgggatt ttagggacgg cttttgctat tacagctgga
    60961 gccggtacag aaaccgcgct tttactaggt ttgccgattg ctacgttgac attgatattg
    61021 aaaaatattt acttagggct tttgattcca attatgaacc ataaagcaga caattatgca
    61081 gaagaaggaa attataaagg agtcgagcat atgcacttgc ttgccggtat cggtctttcc
    61141 ttgatgcttg gtgttgtggt aatggtttct tatatggtgg gaagcaatac gatcggaaat
    61201 ctattaaata tgattcctga cttcgttcaa aaggggttgt cagtagctac agggttgatt
    61261 ccagctcttg gttttgcgat gcttgctcgt ttgctgatca acaaacaagt tgcaccatat
    61321 tttttcttag gttttgctat agcttcttac ttagagattc ctgtgaccgg agtagctatt
    61381 tttggtgcga ttttagcagt tgtggtagtc aatattatga atgtccgtca actaccactt
    61441 caaacaactg caggggaggt agaagaagat gaagaattct gagatcaaaa acagcaaaat
    61501 aacgaaaaaa gagctaaatc acgtattttg gcgttctttt caaatggagt tttcttggaa
    61561 ttatgaacgt cagatgaata tggcttatgt ttatgcaatg attccgattt tgaaaaagtt
    61621 atatcagtcg aaagaagaaa tggcagcagc tttgaaacgg catctagagt tttttaacac
    61681 gaccccacat atcgttacac tgattttagg aatcacggct gcaatggagg aagaaaataa
    61741 ggatgatcct gaatttgatg tgactgcgat cgatagtatc aaaacatctt tgatgggacc
    61801 tcttgcaggg ttaggagatt ctttcttttg gggaactttg cgattgattg ccacaggtgt
    61861 gggtacctct ttagccatgc aaggaaatat tttaggccct atcttgttca ttttgatttt
    61921 caatattccg cacattctgt ttcgctatct agcaacaggt tggggatatc gcttaggaac
    61981 gggttttttg aaaaagatcc aggcaaacgg tatgatggaa agtttgacat taggcgcgtc
    62041 tattattgga ctgatggtcg ttggagcaat gactgccacg atgatcacca tcaatattcc
    62101 tttaaaaatc ggttcaggag aaaatgctgt gactgttcaa agtatttttg atgatatcgt
    62161 tccaaatatc ttatgtcttg gagcatttgg catagtgttt tatctattga agaaagaagt
    62221 gaaaccatta acgatcttga tcggactagc tttctttgga atttttggtt cgtggatagg
    62281 gattttttaa aaaaggagca gagaaaatgt ttacgatgca agattatatc tatgaagaaa
    62341 aggaagtact aagtacgatc ttgaaaaaga atgatttttc gacaagagaa catatgaaaa
    62401 aaacagtgaa tttactgatc ttagcaacgg gttcatctta taacgcttgt ttagcagcga
    62461 agccagcatt ggaatcttat ggtgatctta ctgtcgatat tcaagaacct ttttattttt
    62521 tcaattatgg aaaactttct ccttcaatcg atactgtgat tgctgtgtca caaagcggga
    62581 aaagtgcttc tactgttgaa gcagtaaaaa tgatacaaaa acaagggtta cctgttgttg
    62641 ctatcacaaa tgacgtgcaa agtccacttg ctttagaagc tgcacaaatt attgatttag
    62701 gtgccggggt agaatcagta ggcttcgtta caaaaggcta ttctgcgact gttttacagt
    62761 tactactctt agggatagga attggaataa gtaaaaataa aatcagcaaa aaaatagaac
    62821 aagactacat gcaacagttg cgcaaaataa tcaaccattt accagcgatt attcaaaaaa
    62881 cggaagaatt ttttgatgga tatcaaagtt tatttcgttt agcacagcgt ttcgtcacca
    62941 ttggatatgg tccaaactgg ggaacagcta aagaagcaga gacgaaattg actgaaacaa
    63001 tccgcgttcc gtcacaagga tttgagttag aagcttatat gcacggccca tacctggagg
    63061 cagatgcttc gcatttgcta ttctttattg aaggggaaag tgtaaataag gaacgttcgc
    63121 aaaagttgca aagatatatg agtcggtacg ttggagagac tctcaccatt accacaaaaa
    63181 aagcaagaaa cgagaaaaca cttggattag caatagaatg tgatgaatat ctgtctgtat
    63241 tagcgcttgt cgttccgttt caactattcg cttataaaac cgcagtagct aaaggtattg
    63301 acttgaacaa aaagattttt gaagactttg atacagtctt gaaaagtaaa ttataagagg
    63361 tggaaatcat gctgaaattc aatgaagaag aacaaatcaa agcaataaaa ggcgcactag
    63421 ctttacgtcc gcaagtagaa gaaatcatcg ataaacttta tgcggaaaaa tttgacgctg
    63481 tctattatct aggaatcggt ggaacgtatg cttcctcgat gcaagctgtc acttatatga
    63541 atgggaaaag caacttgcct gtctttgtac agcatgctgc agaatattac actactggaa
    63601 acaaaagatt aacaaaagat tctatcgtta ttttgtcctc tgttaccggt acaactcaag
    63661 aagtagtgaa agcagtagaa caaatcaaga aagtaggagc gacactgatt ggatttatcg
    63721 ataaagcgaa cagtaagttg tctcagctat gcgattttgt ggtcacttat ccagctcctg
    63781 gaacagaaca aatcaaattt tttatggcag cagatcgatt gatgtatttg aatggagaat
    63841 tcgaagctta ttcagaatac tatgaacagc tcgaacgtta tttaccaaca gggttagtgg
    63901 aagcagagaa aaaagcagat gcatttggct tatcatttgc agaaaaacat cgacgtgata
    63961 gtatgcatta ttttattgga gcagggaatc aatggggagc tgtttattca tatgccatgt
    64021 gttactggga agaacaaagc tggcttccat cgaaatctat ccatgctgcc gagtttcttc
    64081 acgggacgtt agagatcgta gaagaaacaa ccccagtcac attattttta ggcgaagatg
    64141 aacagagagt gttatctgaa cgtgtggcta aacttttgcc aaaaatttgc agcaattata
    64201 cattgataga cacaaaagac tatccagtag aaggtatcag tgaaaaatat cgcggacgtg
    64261 ttttgtcttt cttattgatg catgtagtga cacaacgcat cgatgctcat gtggagcagt
    64321 tgaactgcca tccattagag atacgccgct actatcgtca gtttgattat taggatagac
    64381 tggataaaaa gaatataaaa aataaagaga gcagcgataa ctgctccctt tattttttta
    64441 aaatattggc atcaattacg ttataaaaca cattttttgc attatctaca gttcaactta
    64501 taatctgttt aatgggacat gtgggtgcca accatttttt gttatgcaat agtactattt
    64561 agttgttgca tggatccgta gtaaaagtcc aagtacaacg agttgcgcca gtttacatag
    64621 cgttttgtat ccagaatgtt gactaaaaca aaagttccta tgtcaaccgt caacagagac
    64681 aatctgtccg ttgcaggacc tgacagattt agaaatgttc aatctgtctc ttatgtattt
    64741 taaatcaata ctcactagga gaagtttcct aacctcctaa tagtaatgct ggtagtaaca
    64801 ttgtcaaaac tgcttcctct taggaacaag caaagcataa cgagtgtaaa aataaaatgt
    64861 agactattgt aacatatcat tgattatttc aagtaaaaac gataaaagaa gtaaacaaac
    64921 acttaaaaac aatggaaatt agtcccaact ttaagaaatg tatactataa tggaaattat
    64981 caattattta tttggaggaa tatccatgaa acaaaaaatc gcagttcttg gtcctggatc
    65041 ttggggcact gctttagcac aaacattggc agaaaacggg catgatgtcc gtatttgggg
    65101 aaatgtacct gaacagattg atgaaatcaa taagtatcat acaaatcagc attttttacc
    65161 tgatttgacg atcccagaat cgattatcgg atataaagaa ttggcagaag cagtggataa
    65221 cgcagatgct attttatttg ttgttccaac aaaagcgatt cgttcagtcg cacaggaact
    65281 tattggtaaa atgaatacta agccaatcat tatacatgca agtaaaggac tagaacaaga
    65341 tacgcataaa cgaatctcag aagttttggc tgaagaaatt ccagaagaaa aaagacaggc
    65401 aattgttgta ttatctggtc ctagccatgc tgaggaagtg gcagtacatg atatcacaac
    65461 aattacagct gcaagtattg atcaaaaagc agcagcctat gtacaaaaac tgttcatgaa
    65521 tgattatttc aggatttata caaataatga tgtgattggc gtagaaactg gtgcagcact
    65581 gaaaaatatc attgcaattg gtgccggcgc tatccatgga ttaggattcg gagatgatgc
    65641 aaaagcagca atcatgactc gaggattggc agaaatcagc cgtcttggtg tcgcgatggg
    65701 tgccaatcct ctgacattta tcggtttgag tggtgtgggg gacttgatcg ttacttgtac
    65761 aagtgttcat tctcgcaatt ggcgtgccgg taacttgtta ggacagggac ataagctgga
    65821 agaagttcta gaaaacatgg gaatggtcgt tgagggtgtg gcaacgacaa aagcagcagt
    65881 agagctagct caacagctag gtgttgagat gccaattact caaacaatct ataatgtttt
    65941 gtataatgga gaagatatca aaaaagcagc aaaagaaatc atgttacgtg acggaaaaat
    66001 ggagaatgaa ttttctcttc gttaaataga tgatcaatag ttaatcgt
//
LOCUS       DACDXO010000007        61576 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E23605_2017
            SAMN12639989-rid9881463.denovo.007, whole genome shotgun sequence.
ACCESSION   DACDXO010000007 DACDXO010000000
VERSION     DACDXO010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639989
            Sequence Read Archive: SRR10023389
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 61576)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 61576)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741914.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:42:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,972
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 33 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 17 of 73
            Pseudo Genes (multiple problems)  :: 10 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..61576
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639989-rid9881463.denovo.007"
                     /isolate="E23605_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..2327)
                     /gene="esp"
                     /locus_tag="IWM35_000574"
     CDS             complement(<1..2327)
                     /gene="esp"
                     /locus_tag="IWM35_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repetitive surface protein Esp"
                     /protein_id="HAP9918450.1"
                     /translation="MVSKNNKRVFLEKTKKRVLKYSIKKLSVGVASVLIGVGLVLGTT
                     ELVKAQDEISPSSPLETAISSVQVGDKVASGNTFQENPGYTKNYNFSDLQFSPQELTG
                     DTLKGNTIGFEVYGKHNIAASTKNWEIRLQLDERLAKYVEKIQVDPKKGIGSSRRTFV
                     RINDSLGRPTNIWKVNYIRASDGLFAGAETTDTQTAPNGVITFEKSLDEIFKEIGIDN
                     LKTDRLMYRIYLVSHQDDDKIVPGIDSTGYFLTDSDDFYNSLDVSENNPDQFKHGSVS
                     AKYEKPNTQTKDGSGSTGANGAIILDHKLTKNYNFSYSSSAKGTPWYANYKIDERLVP
                     YVAGIQMHMVQADKVTYDVSFESGTKVADLAIERREGHENYGIGSITNNDLTKLIDFA
                     NASPRPVVIRYVLQLTKPLDEILEDMKATAQVEENKPFGEDFIFDSWLSDTNKKLIQN
                     TYGTGYYYLQDIDGDGNPDDKEESGDTNPYIGKPELEEVYDVDTTVKGKVFIHELAGT
                     GHKAQLVDKEGTVLAEKTIAPNEKDGAPISDTVEFEFTGVDSSKLIAKDELKIQIVSP
                     GFDKPEEGSTVIKESPKAVDKQTVVVGFKPDAKESIRNNKNLPEDAEYSWKTEPDTSN
                     VTDSTKGIVTVKIGNRTFDVDVEFAVKASQAMENDATYVPITTTPETTVQSGKPTFDK
                     PDVPLAKDAFSISDVYNKYFGNASVDANTGIVTFTPAKGVGESEPITGTIPIKIVYQD
                     GSVGTTDLAVTVSKDIYENPGENIPAGYHKVTFTAG"
     gene            complement(2944..3378)
                     /locus_tag="IWM35_000575"
     CDS             complement(2944..3378)
                     /locus_tag="IWM35_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9918451.1"
                     /translation="MRLYHLRSLEELRKLDFNKKFFWEISDLSCFVESERLSNGNNSV
                     LNEYDKVVINKVKSLCEEARESISHINGSNFKQTLFNLIKLDAKISSYLFFLIHDEFM
                     DYQKLDQLIERESWEDYYVGLTFSEKLNNYKLIDYKYLSESN"
     gene            complement(3395..4600)
                     /locus_tag="IWM35_000576"
     CDS             complement(3395..4600)
                     /locus_tag="IWM35_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HAP9918452.1"
                     /translation="MFSDKEVLLKSLHVCLKLPIVVFNSEFSIIKEYRSDRTTYFFYD
                     YKKLLTDNIKKTKDFHFFTGDFNEMFLLYMFKTRYYLFGPFRANNIDKVFFKLKMNNL
                     NVAMADRERLYNSLQNLTLYSLGDIRDILILVHYFFTGKIEDLFHEPLIEYTENLSKT
                     IEQIKIDNLLSQNYDPEIYLFLYENKILEYVKNGDIRNLENMVFNLSNGIIPSVSGDT
                     IRSEKNYSIIVFEKLAQTSITLGMDIIEAYQSRDALIQENELAVSLPEVLKVRDSGIV
                     YYTKEIGKTKIEHLSPLISSVVQFIGLNIYKRITVKEIANYFSVSETKLRESFKNEMH
                     ITIYNYISKRKISTAKIMLKSNHTISEVSLGLGFSDSSHFSRVFKKYAGVSPKQYQLG
                     LVDNFNNIS"
     gene            5294..5680
                     /locus_tag="IWM35_000577"
     CDS             5294..5680
                     /locus_tag="IWM35_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAP9918453.1"
                     /translation="MSDNDERVNAQLIRFFEKVILNTANTYYKKQEEIQKHEQLDQLP
                     PYLFTKDTIKFKQPINVLNVTLLVEDIKLAKILPLLKEPEQIFLIEKFIFDKTDKEIG
                     KVLGITRQGATNLKHRLYKKIQKMLV"
     gene            5774..5965
                     /locus_tag="IWM35_000578"
     CDS             5774..5965
                     /locus_tag="IWM35_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9918454.1"
                     /translation="MNSLKEDFILAKAGNEEAVEAILKRFSSLIHKQSWRTGKYDQDC
                     YQECMLAIYLAISKFEIKE"
     gene            6479..6811
                     /locus_tag="IWM35_000579"
     CDS             6479..6811
                     /locus_tag="IWM35_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP9918455.1"
                     /translation="MRKTYDRKFKLKISQDILEKKVTTKKIAEEYNISRPTISRWVSE
                     YRRYGKNAFAGQGKRLPDKADFYILEQENKRLKEEDECSTNTKIDTDLQGSLKNFTMK
                     ESKSINVL"
     gene            7107..7262
                     /locus_tag="IWM35_000580"
     CDS             7107..7262
                     /locus_tag="IWM35_000580"
                     /inference="COORDINATES: protein motif:HMM:NF012870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HAP9918456.1"
                     /translation="MPTVIHSDRGSQHRSFIYQELLNDHHITHSVSQTGTPVDNAVIE
                     AFHRSIK"
     gene            7538..8878
                     /locus_tag="IWM35_000581"
                     /pseudo
     CDS             7538..8878
                     /locus_tag="IWM35_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(9119..10735)
                     /locus_tag="IWM35_000582"
     CDS             complement(9119..10735)
                     /locus_tag="IWM35_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAP9918457.1"
                     /translation="MKKIPITILTGYLGSGKTILLNHILYGDHGKEIAVIVNDIGEVN
                     VDGVLVDKKGFKRTGEKLVSMPNGCICCTLREDLIVELDQLVQFSDLNQIVIEASGIS
                     KPIPIIQAIILAKNPLDIDLTQRLEIGAIIIVVDVKRFWDNFETGEKIQERKAEGNVV
                     EEEQDIRGLLIDQFECCNILLLNNSDLVSTKTIYKINGLIAKLQSNAKIIRTIKGEVD
                     LSKILNTNLFNIDETSYSVGWFKELELGYQNHIPETEEYGISSFIYRNRLLLDAQKFA
                     HLTDKNFPGNITRAKGFLWFAQHSDFSTIFETSGSYKSIESLNYWVASLPKIECQMII
                     QKNPELKEAWHPLFGERINKLVFIGMKMDQQCIKEQLEGCLTDSLNADNFRKISPFPW
                     KKSNMFSQISLMKFLRKAGVKIGDVIIIGILIITSFIPLFVFSIEKENETTEDAIKYA
                     IVRIDGKEMDRFDLDKINHKLVNYHPAKGQYNIVEIKDGRIRVKEDNSPDQIAVRTGW
                     ISKPGQTSICLPHKLVISIEKKESTDYYIY"
     gene            10853..11071
                     /locus_tag="IWM35_000583"
     CDS             10853..11071
                     /locus_tag="IWM35_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9918458.1"
                     /translation="MSQYRHLTILERERIFLLHEEGFSISIIGNDIQHSPFTILHELR
                     RNKKKTIHLPKRKKIMLIEEKNADVITF"
     gene            11266..11727
                     /locus_tag="IWM35_000584"
     CDS             11266..11727
                     /locus_tag="IWM35_000584"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP9918459.1"
                     /translation="MHHRGKTRHTKNHVESRGKICLTHSIHGRPKSADDRSDFGHWKA
                     DIVAGKTGGTCLVTLTDRKSCFLLAGKKVPRKKAAYVSEKMIELLSSIPNEAVKTITP
                     DRGKEFSNHFAVTEALNNMPFYFPDPHALGNNEQIKIRIDSFENTCQNRKR"
     gene            complement(11920..12159)
                     /locus_tag="IWM35_000585"
     CDS             complement(11920..12159)
                     /locus_tag="IWM35_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9918460.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYRFAKQMRLNGSTLADIAATLHVSI
                     DTVHRVLKRDPDCCFIKVNTGRSRRLAKRHNSLAN"
     gene            complement(12183..13706)
                     /locus_tag="IWM35_000586"
     CDS             complement(12183..13706)
                     /locus_tag="IWM35_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HAP9918461.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIVGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKAKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGYLALD
                     YGLKQVPIAGLSPEEEPSSGRLAELKEYVKKNKINYIYFEKNANDKIAKTLANEAGIK
                     LEVLNPLESLTKEQMDNGEDYVSVMEDNLKALEKTTMVAGEEVVPEKEAKDEKTVANG
                     YFKDADVKDRELSDYTGEWQSVYPLLKDGILDEVFDYKAKINKDMTAAEYKDYYTTGY
                     KTDIDTINIKDNTIDFVVNGEHHQYTYKYVGYKILNYEKGNRGVRFNFETDDAGAGRF
                     KYIQFSDHGIAPSKAEHFHIFFGGESQEKLYNELHNWPTFYPASLSKHEIAQEMMAH"
     gene            complement(13724..13846)
                     /locus_tag="IWM35_000587"
     CDS             complement(13724..13846)
                     /locus_tag="IWM35_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HAP9918462.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKRNKMKKLA"
     gene            complement(13876..14859)
                     /locus_tag="IWM35_000588"
     CDS             complement(13876..14859)
                     /locus_tag="IWM35_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAP9918463.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHSEERVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVMADRIFVSRK
                     QNLNLSALKKLQREIKSINPLSDIRMFTHSETIQKKDFYNLKKFNHPLVFESDSSENH
                     YNGKYYHQHHQVHEFKSILLTSDQDIKKDFLLQWIDWLILTNQGSLYRFKGLIALQEQ
                     DFIVAMQEVNEQVNFQYTTRQKDQTTIILIGKMLDEERIKQSFDKLLAESK"
     gene            complement(14883..15032)
                     /gene="rpmG"
                     /locus_tag="IWM35_000589"
     CDS             complement(14883..15032)
                     /gene="rpmG"
                     /locus_tag="IWM35_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003550836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAP9918464.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSPKLRRRAI
                     FKEVK"
     gene            complement(15053..15430)
                     /locus_tag="IWM35_000590"
     CDS             complement(15053..15430)
                     /locus_tag="IWM35_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9918465.1"
                     /translation="MEIIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITETGL
                     PTQQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLV
                     SQLESGELTFEDAQQIQAYLEDR"
     gene            complement(15462..15641)
                     /gene="rpmF"
                     /locus_tag="IWM35_000591"
     CDS             complement(15462..15641)
                     /gene="rpmF"
                     /locus_tag="IWM35_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HAP9918466.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVIKGASK"
     gene            complement(15656..15925)
                     /gene="rpsN"
                     /locus_tag="IWM35_000592"
     CDS             complement(15656..15925)
                     /gene="rpsN"
                     /locus_tag="IWM35_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HAP9918467.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
     gene            complement(16448..18220)
                     /locus_tag="IWM35_000593"
     CDS             complement(16448..18220)
                     /locus_tag="IWM35_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002841847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9918468.1"
                     /translation="MRMQMIGGLTMKSFYKNKFALSDQGAEALTKASISSFFVYCINM
                     VPAFIIMMLIDELVLENTKPRWLYFAVSFVTLLFMYWLLDREYENLYNSTYKESAHLR
                     VQIADDLSNLPLSYFSKHNLSDLSQTIMSDVEGIEHAMSHAIPKAGGMALFFPFISVM
                     LLVGNVKMGLAVILPTLFSFVLILLSKKSQTKANTKYYDTLRENSEEFQETIELQQEI
                     NSFNLSKKVQDRLFKKMEESERIHLKVELSTFSVMALSSIFSYVSLAVVILVGVHLLL
                     TGEVTILYVVGYLLAAIKIKDSFDSMKEAVLEIFYLAPKIQRIRAMKETSIQEGSDSP
                     LKSFDIELRDVSFSYDNNTPILDHISFTAKQGEVTALVGASGSGKTSILKLVSRLYDY
                     DEGCILIDGYDIKRVSPASLFSKIAIVFQEVTLFNTSILENIRIGNSQASDEEVKKAA
                     RLANCEDFIEKLPDGYHTLVGENGSSLSGGERQRLSIARAFLKNAPILILDEITASLD
                     AENEKRIQESLNRLIQDKTVLIISHRLKSIEKVNKIVVMDQGKLVDQGTHNELYRRSE
                     IYKNLIKKTKLSEKFVYEKEAQSR"
     gene            complement(18174..19928)
                     /locus_tag="IWM35_000594"
     CDS             complement(18174..19928)
                     /locus_tag="IWM35_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9918469.1"
                     /translation="MKVYKKLFSYVPKMKYYGWLATLFSDFSVVLTVYGYYSIYKFLQ
                     SLIIVGDEYLAKSYAVQTVAWLTLGALFYIFSGLFSHILGFRLETNLRKKGISGLTEA
                     SFRFFDLNSSGYIRKTIDDNAAKTHMAIAHLIPDSAQAIVTPVLSVALGFFINFKIGL
                     VLIAMLVIGVFLLKKMTENTNFIQKYQESLDVLSSETVEYIRGIQVIKIFGAKVTSFK
                     ALNKAINDYAKFAYKYSLSGKTPFVLFQWLFFGIVCILIVPITFFITSLTTPKILAVD
                     LLMLFFLSGIIFVSFMRIMYVSMHLFEARYAVETLDSLYLEMKQDQLKYGNETQIKNC
                     DIAFENVSFSYKEECVLENFNLVLEQKRIYALVGKSGSGKSTIAKLISGFYNVDKGQI
                     KIGGKPLNHYSKDTIINTIAFVFQDTKLFNQSIYDNVSIANPQAPASKVFDALRLAGC
                     ESILEKFPEREHTLIGSKGVYLSGGEKQRIAIARAILKDAKIVIMDEASASIDPDNEY
                     ELQKAFQHLMKEKTVIMIAHRLSSIKSVDEILVLSEGKIVERGNHNELMAKHQVYKQL
                     VTNYENANDWRLDNEKFL"
     gene            complement(20038..20607)
                     /locus_tag="IWM35_000595"
     CDS             complement(20038..20607)
                     /locus_tag="IWM35_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HAP9918470.1"
                     /translation="MKTKDYIFIGLATVISLVIYFFSIMVSSIGGAFGHSISPGIFGL
                     LSGIIFVYISYNYPRKGIFTIYTIVLLLFFTLMGGAYLPWFISSISMAILADIILSVF
                     GYDRAIPQVASWALMQLGSAAGQWIPIWFFTDRFRQDWIDKGQSAATMDAMIHYAVGI
                     WGIISVLVVASLSMIGVLIGRKVLKKYKK"
     gene            complement(20625..22049)
                     /locus_tag="IWM35_000596"
     CDS             complement(20625..22049)
                     /locus_tag="IWM35_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAP9918471.1"
                     /translation="MKELFEFNNVSFSYKDVKEQALVDLNFSIKKGSLTLITGSSGSG
                     KSTLLKLMNGLIPRLYDGKLVGEILFENNSLQSLTQESISKNIGYVSQDPRGQFFTTN
                     TTSELVFSMENFGFTKEHMDKRINELAELLDLSNILDKNIFSLSSGERQKISIGCSLS
                     LNSKLIILDEPSSNLDFKATKKLADLLCDLKNKGFTIVIAEHRFYYLKELIDQVLFVK
                     NRTVKTTTIQLLKTENNNALRKFDIFNSSIEVPYIEKVSADVLKVEEITYENILKDIN
                     FDVKVGDVIGLVGKNGVGKTTLLRILMKIIRPTKGKIIENKKNLSSPFLVMQEMDYQF
                     FTESVRSELALGNEIVSKDQQEKILKKMELYKMKDQLPFDLSGGEKQRLLVSIASLSK
                     TNLFLFDEPTSGLDYINMERIADLIKDLKEKGAVVIATHDPEFLYKTCNRIIYLKNGG
                     IKKDIKLRVEDSTLIEQIFNDITK"
     gene            complement(22051..22719)
                     /locus_tag="IWM35_000597"
     CDS             complement(22051..22719)
                     /locus_tag="IWM35_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HAP9918472.1"
                     /translation="MSKINPLTLITLNIFFPVIIFLGKGNIFQVGCFIIATVLVLMFR
                     KIKTCIYFILIYSVLFLVMKIIDAIKIEFFTIFFATSLYIVFRMLPVVMISWLLVSVY
                     TSNELLSSLEKIHLPKKIMLSITVVIRFFPTYRSEIRMIKESLKMRNIRLSFFQPLQY
                     LEFLLVPVLIRATMIAEEMTANAITKGIESPVKRSSYYKTNMSLLDYIILFSVTIFFL
                     SILI"
     gene            complement(22849..23433)
                     /locus_tag="IWM35_000598"
     CDS             complement(22849..23433)
                     /locus_tag="IWM35_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP9918473.1"
                     /translation="MESGLSHFEKEGFQQTKIRQLCLTAGVTTGAFYKHFTSKEALFE
                     EIIQPHINALINMYNEEFTKYLLNLENIDIEKSWRENFNELDPFINYIFENKRIFDLI
                     LFQSAGTKYQDIVEKITSFVTKKTVCCLDKLKTIGMINQNLVFNEEEIHFYIYSFYAT
                     FYDILKHSYSKEKTMLLTRRLYQFSVPGWIELLS"
     gene            complement(23764..23997)
                     /locus_tag="IWM35_000599"
     CDS             complement(23764..23997)
                     /locus_tag="IWM35_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dependent repressor"
                     /protein_id="HAP9918474.1"
                     /translation="MVQIKTTTILIYLLAIQELSKRKKKLKNNDLAKVLSVSPASVSE
                     MLAKLRYEDYLDIGFQLTDKGKKFIDDYKKGFK"
     gene            complement(24012..24962)
                     /locus_tag="IWM35_000600"
     CDS             complement(24012..24962)
                     /locus_tag="IWM35_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HAP9918475.1"
                     /translation="MMRKWKAVLGSLGILIALFIFGACSINSKDKDKVASNEKLKVVV
                     TNSILADITENIAKDKIDLHSIVPIGKDPHEYEPLPEDVQKTSKADLIFYNGVNLETG
                     GNAWFTKLVKNANKEENKDYFAASDGIDVIYLEGQSEKGKEDPHAWLNLENGIIYAKN
                     IEKQLAEKDPDNKKFYKENLDKYIEKLDSLDKEAKSKFASIPNDKKMIVTSEGCFKYF
                     SKAYNVPSAYIWEINTEEEGTPDQIKHLVEKLRTTKVPSLFVESSVDDRPMKTVSKDT
                     NIPIYSTIFTDSIAEKGQDGDSYYAMMKWNLDKIAEGLSK"
     gene            complement(24959..25801)
                     /locus_tag="IWM35_000601"
     CDS             complement(24959..25801)
                     /locus_tag="IWM35_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAP9918476.1"
                     /translation="MLQNFINGLYDFHFLQNALITSVVIGIVSGTVGCFIILRGMSLM
                     GDAISHAVLPGVAISYILGINFFIGAIVFGLLASIIITFIKSNSVIKGDTAIGITFSS
                     FLALGVILIGVANSSTDLFHILFGNILAVQDIDKWITIGVSVLVLVIVVLFFKELLIT
                     SFDPLMAKAIGMNVSFYHYLLMVLLTLVAVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKTMIFISATVGASSSLLGLFIGYSFNIAAGSSIVLTAAFLFVISFLISPKQKFLRKK
                     ERSL"
     gene            complement(25803..26522)
                     /locus_tag="IWM35_000602"
     CDS             complement(25803..26522)
                     /locus_tag="IWM35_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAP9918477.1"
                     /translation="MLEVKKLNVCYNDFLALNEISLKIQEGALTGIIGPNGAGKSTLL
                     KAMLNIIPHQGEVSINNEDINKKLVKIAYVEQKADIDFTFPIKVKECVSMGTYAGMKV
                     FQRIKNAEWQRVSKALEKVDMGKYSNHQIGELSGGQFQRVLLARCLAQNADFIFLDEP
                     FVGIDLVSERIIMDTLKKLKHQGKTVLIVHHDLSKVKKYFDNIILLNRNLIAHGSVES
                     VFNEENLKKAYGDTIFIGEGE"
     gene            27214..27372
                     /locus_tag="IWM35_000603"
     CDS             27214..27372
                     /locus_tag="IWM35_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9918478.1"
                     /translation="MNKKQTCAFLGIDNNTLDKWIRIYNFPVIRVGGVVRFDVEDIKD
                     FMNTFKIT"
     gene            27444..28670
                     /locus_tag="IWM35_000604"
     CDS             27444..28670
                     /locus_tag="IWM35_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP9918479.1"
                     /translation="MPTKLSNGKYKTNLRYPKRFKEITGIASEKYQKTFPNRQLAIKA
                     ENDMKKKIEKVLREENANSLELKGKITFKKFYESKWLPRYELGQTIRSNRPPSDITIS
                     NTKDIFRLHILPMFGEYAMNYLNINTEIISDELTKKSKEYANIKIIKGYVRSMFDIAE
                     ILNYIEFNRTTKIIQSITAPKKNALEEKRIQEGKQALSSKELTNWIEAVNDDFNNHLL
                     TFHDYTLFMITLYLGDRKSETYALQWKYIDFEKQTVRLKHTLDKYQRKKFTKGRKDTV
                     IQVPEVVMTLLSEWKSVQADQLLKLKIKQTLDQYLFTYTKPSGEVNCPVHADYLNYRI
                     NSIKRRHPDLVHLSPHKLRHTYATIARQGGADMNQISNALTHSDISTTKIYVNTPDIV
                     DKAVFEAFQRGLNKCD"
     gene            complement(28758..29150)
                     /gene="rpsI"
                     /locus_tag="IWM35_000605"
     CDS             complement(28758..29150)
                     /gene="rpsI"
                     /locus_tag="IWM35_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HAP9918480.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(29166..29609)
                     /gene="rplM"
                     /locus_tag="IWM35_000606"
     CDS             complement(29166..29609)
                     /gene="rplM"
                     /locus_tag="IWM35_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HAP9918481.1"
                     /translation="MRTTYMAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPT
                     FTPHLDTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLI
                     ETSVKGMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            complement(30128..30595)
                     /locus_tag="IWM35_000607"
     CDS             complement(30128..30595)
                     /locus_tag="IWM35_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HAP9918482.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            complement(30715..32253)
                     /locus_tag="IWM35_000608"
     CDS             complement(30715..32253)
                     /locus_tag="IWM35_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HAP9918483.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            32333..33019
                     /locus_tag="IWM35_000609"
     CDS             32333..33019
                     /locus_tag="IWM35_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HAP9918484.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            complement(33220..33747)
                     /gene="msrA"
                     /locus_tag="IWM35_000610"
     CDS             complement(33220..33747)
                     /gene="msrA"
                     /locus_tag="IWM35_000610"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAP9918485.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(33831..34508)
                     /gene="pgmB"
                     /locus_tag="IWM35_000611"
     CDS             complement(33831..34508)
                     /gene="pgmB"
                     /locus_tag="IWM35_000611"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HAP9918486.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            complement(34505..35635)
                     /locus_tag="IWM35_000612"
     CDS             complement(34505..35635)
                     /locus_tag="IWM35_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAP9918487.1"
                     /translation="MRILIAPDSFKESLTALEAAQAIQSGFQEVFVQAEYDLLPIGDG
                     GEGTVSSLADSLRLKRQTAVVTNAFGKNKEIQYASDGQTAIFEMAEIVGLKDVPLNQQ
                     QPLRLSTRGVGELIRQLLQQGVRHIIIGVGGSSTNDGGIGMAEGLGYAFYDQDNQKVE
                     ALGENLGKIATVDKTNVPIELIKAKITVITDVTNLLCGKEGASFVFGPQKGLKENELA
                     VADQAMHDFYMRFAPQVIEASGSSAGGGMGAGLLAFASGKIVSGIDYVLDLLEFDKRV
                     QQADLVIVGEGRLDGQSLKGKAPVGIARRVPEGVPVIAICGSVGKGSEKAADVGISAV
                     FPILPAAVPLQEALQQATDNLHRTAKNIASLLQVVHFQGGNR"
     gene            complement(35635..37818)
                     /locus_tag="IWM35_000613"
     CDS             complement(35635..37818)
                     /locus_tag="IWM35_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HAP9918488.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            complement(37815..38840)
                     /locus_tag="IWM35_000614"
     CDS             complement(37815..38840)
                     /locus_tag="IWM35_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP9918489.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTPNRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            complement(38885..39712)
                     /locus_tag="IWM35_000615"
     CDS             complement(38885..39712)
                     /locus_tag="IWM35_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAP9918490.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            complement(39709..40776)
                     /locus_tag="IWM35_000616"
     CDS             complement(39709..40776)
                     /locus_tag="IWM35_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HAP9918491.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKHGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLIGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            complement(40802..41638)
                     /locus_tag="IWM35_000617"
     CDS             complement(40802..41638)
                     /locus_tag="IWM35_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP9918492.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            complement(41635..42504)
                     /locus_tag="IWM35_000618"
     CDS             complement(41635..42504)
                     /locus_tag="IWM35_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP9918493.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            complement(42574..43857)
                     /locus_tag="IWM35_000619"
     CDS             complement(42574..43857)
                     /locus_tag="IWM35_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAP9918494.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            complement(43871..45556)
                     /locus_tag="IWM35_000620"
     CDS             complement(43871..45556)
                     /locus_tag="IWM35_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphorylase"
                     /protein_id="HAP9918495.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGARVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            45831..46856
                     /locus_tag="IWM35_000621"
     CDS             45831..46856
                     /locus_tag="IWM35_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP9918496.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
     gene            complement(47451..51104)
                     /gene="rpoC"
                     /locus_tag="IWM35_000622"
     CDS             complement(47451..51104)
                     /gene="rpoC"
                     /locus_tag="IWM35_000622"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HAP9918497.1"
                     /translation="MIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYRTLKPERE
                     GLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHIELAAPVS
                     HIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTEREYREKRE
                     QYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLDILEAFRA
                     SGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLKRLLDLNA
                     PGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKNAKRKIER
                     QEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVCEAYNADF
                     DGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYYLTMEEEG
                     REGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMITTVGKII
                     FNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKKKNLGNII
                     AEVFKRFKVTETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIENAHKQVET
                     ITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSGARGNISN
                     FTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTALKTADSG
                     YLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTRKEVRHPE
                     TNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNLATGSDVE
                     VGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNPKGQAVIT
                     EVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGAPLTEGSI
                     DPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRVMDPGDTD
                     ILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAASFQETTRV
                     LTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVYSISDIEA
                     QMAAEDAMNNQTK"
     gene            complement(51183..54800)
                     /gene="rpoB"
                     /locus_tag="IWM35_000623"
     CDS             complement(51183..54800)
                     /gene="rpoB"
                     /locus_tag="IWM35_000623"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HAP9918498.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLSALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            55528..56496
                     /locus_tag="IWM35_000624"
     CDS             55528..56496
                     /locus_tag="IWM35_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP9918499.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            56514..57419
                     /locus_tag="IWM35_000625"
     CDS             56514..57419
                     /locus_tag="IWM35_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9918500.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGGAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            57416..58222
                     /locus_tag="IWM35_000626"
     CDS             57416..58222
                     /locus_tag="IWM35_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP9918501.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            58379..59347
                     /locus_tag="IWM35_000627"
     CDS             58379..59347
                     /locus_tag="IWM35_000627"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HAP9918502.1"
                     /translation="MTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKAIKELEKA
                     GYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMLTSESTMKDAKLAAAQGD
                     SVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTIIAASACVK
                     AIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLNFSVPQNH
                     FPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKSFVLGKTV
                     SFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(59664..60188)
                     /locus_tag="IWM35_000628"
     CDS             complement(59664..60188)
                     /locus_tag="IWM35_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HAP9918503.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            60475..>61576
                     /gene="scm"
                     /locus_tag="IWM35_000629"
     CDS             60475..>61576
                     /gene="scm"
                     /locus_tag="IWM35_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HAP9918504.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSSTSSDSTDTTSTT"
BASE COUNT        18685 a        12360 c        10309 g        20222 t
ORIGIN      
        1 cctgcggtga aggttacttt gtggtagcct gcaggaatgt tttctcctgg attttcataa
       61 atatctttac ttacagttac tgctaaatcg gtcgtgccta cagaaccatc ttggtaaaca
      121 attttaatag gaattgttcc agtaatcggc tccgattctc ctacaccttt agctggagtg
      181 aatgtaacaa taccagtgtt tgcatcaaca cttgcattac cgaagtactt attatagaca
      241 tctgaaattg aaaaggcatc tttagctaaa ggaacatctg gtttatcaaa tgtaggtttt
      301 ccactttgaa ctgtcgtttc tggagttgtt gttataggta cgtatgttgc atcattttcc
      361 atagcttgag aagcttttac agcaaactct acatccacgt cgaaagttcg atttccgatc
      421 ttaacagtta caataccttt cgtactatca gtaacgttag aagtatcagg ctctgttttc
      481 catgaatact ctgcatcttc aggtaagttt ttattattcc gaattgattc tttagcatct
      541 ggtttaaatc caactaccac ggtttgttta tcaaccgctt ttggtgattc cttaataacg
      601 gttgaacctt cttctggttt atcaaaacct ggagaaacga tttggatttt taattcatct
      661 ttcgcgatta atttacttga atctacaccc gtaaattcaa attctacagt atctgaaatt
      721 ggagccccat ctttttcatt tggagcgata gttttttctg ctaatacagt accttcttta
      781 tcaacaagtt gggctttgtg acctgttccc gctaactcgt ggatgaatac tttcccctta
      841 actgttgtgt caacatcata tacttcttct aattcaggtt tcccgatata tggattcgtg
      901 tctccgctct cttctttatc gtcagggttt ccatcaccat caatatcttg caaataataa
      961 taacctgttc cataagtgtt ctggattaat tttttattcg tatccgacaa ccaagaatca
     1021 aagatgaaat cttcaccaaa tggtttattt tcttcaactt gtgctgttgc tttcatatct
     1081 tctaagattt catctaatgg cttagtcaat tgtaaaacat atcgaataac gactggacgt
     1141 ggacttgcat tagcaaaatc gataagttta gttaaatcat tattagttat tgaacctatg
     1201 ccataatttt catgaccttc ccgtcgctcg attgctaaat cagctacttt tgttccggat
     1261 tcaaacgaaa catcatatgt tactttgtca gcctgaacca tgtgcatctg tatacccgct
     1321 acatatggaa ctaatcgttc atcaattttg taattagcat accaaggcgt gccctttgcg
     1381 gaagatgaat atgagaaatt ataatttttc gttaacttat gatctaagat tatagctcca
     1441 ttagcacctg tggatcctga accatctttt gtttgagtat taggtttctc atattttgca
     1501 cttactgatc catgtttaaa ctggtcagga ttattttcag atacatctaa gctattatag
     1561 aaatcatctg aatctgttaa aaagtagccc gtactatcaa tacccggtac aattttatca
     1621 tcgtcttgat gacttaccaa gtagattcga tacattaaac gatcggtctt gagattgtct
     1681 atgcctatct ctttaaaaat ttcatctagg ctcttttcaa acgtaattac cccattagga
     1741 gcggtttgcg tatcggttgt ttctgcccca gcaaataatc catcacttgc acgaatataa
     1801 ttgaccttcc aaatatttgt aggtctaccc agcgaatcat taattcttac aaaggtccgt
     1861 ctagaagatc caattccttt ctttggatcg acctgaattt tttctacata tttagctaat
     1921 cgttcatcta attgaagacg gatttcccag tttttagttg atgctgcaat attatgtttt
     1981 ccgtaaactt caaatccaat tgtattgcct tttagtgtgt ctccagttag ttcttgagga
     2041 ctaaattgta aatccgaaaa gttataattt ttcgtataac caggattctc ctgaaaagta
     2101 ttccccgaag ctactttgtc acctacttgt acggagctta tcgctgtttc aagtgggctg
     2161 cttggagaaa tttcatcttg tgctttaact agttcagttg ttcccaagac aagaccaaca
     2221 ccaatcaaaa cagatgcaac cccaacactt aattttttga tactgtactt caatactcgt
     2281 ttctttgttt tttctagaaa tactctctta ttattcttgc taaccattat tcaaatcaac
     2341 tcccttaata attactttcc tagtaagaat aaaagataaa tctgatacgt attattcaca
     2401 gaataggaat cttactaacc ttatgttaac taatttactt agagaatttc atgaacatta
     2461 caacaaaaaa agtacatttc ttcatgtttt tttcggatat ttgtgctttt taaccattca
     2521 gcagctgaaa acatataaaa agatatgtat gtattctttc tgaaattaag ttcatttcta
     2581 tgcgatgaag catgttttca aaatattacc ctttcttaga ttaaacttca gagtattcaa
     2641 ataacggaat aaggagcctt atcccgcatt actgaatgag actgtgacat aagtaaaaag
     2701 gtgacttcaa attccttctt cggaatttga aatcaccttc tttataatta taaagtatcg
     2761 gtttcacagc atttctgaat gattcctaga atcttaaacg aatgattgga gttatcaaca
     2821 ttttttcact cgtttttagc gaaacaaaaa atgacatttg tcacagcctc attaaattat
     2881 tgattctttt tctttataat tgaattgtcc atatttaaag tgtctagtac tctacatgaa
     2941 taattaattg ctttcactca aatacttata atctattaac ttgtagttgt ttaacttttc
     3001 tgaaaaagtt agacccacgt aataatcttc ccagctttct ctttcaatta actgatccaa
     3061 tttttgataa tccataaatt catcatgtat caaaaagaat aaataacttg atattttagc
     3121 atctagctta atcagattaa ataatgtttg tttaaaattg ctaccattaa tatgactgat
     3181 agattctcta gcttcttcac ataaactttt tactttatta attactactt tgtcatattc
     3241 atttaacaca ctattattac cattacttag acgctcagat tctacgaaac aactcaaatc
     3301 agaaatttcc caaaaaaatt ttttgttaaa atccaatttc ctcaactcct ccaaactacg
     3361 taaatgatat agtctcataa taaaataaaa cctctcagct aatgttgttg aaattgtcca
     3421 ctaaacctaa ttgatattgc ttaggagata cacctgcgta ctttttgaat actctcgaaa
     3481 agtgtgaaga atctgaaaat cctaaaccta aagaaacttc actgatagta tggtttgatt
     3541 tcaacattat tttagccgta gaaatttttc gcttagagat ataattataa atcgtaatat
     3601 gcatttcatt cttaaagctt tctcttaatt tagtttcact tacagaaaaa taattagcta
     3661 tttcctttac agtaattctt ttatagatat ttaagccgat gaattgaacg acagaagaaa
     3721 ttaagggaga aagatgctct atcttagttt ttccgatttc cttagtataa taaacaatcc
     3781 cagaatccct cacttttaag acctcaggta acgatacagc taattcgttt tcttgtatca
     3841 aagcatctct actttgatac gcttctatta tatccattcc taatgttata gacgtttgtg
     3901 ctaacttttc aaaaacaatg atagaataat ttttttcaga ccgaatcgta tctccactaa
     3961 cacttggaat tattccatta ctcagattaa aaaccatgtt ttctaaattt cgaatatctc
     4021 catttttaac atattccagt attttatttt cataaagaaa cagatatatt tctggatcgt
     4081 aattttgtga taacagatta tctattttga tttgctcgat agttttactt agattttctg
     4141 tatattcaat taacggttca tggaatagat cttctatttt tccagtaaaa aaataatgaa
     4201 ctaaaatcag aatatctcga atgtctccta aagaatacaa agtcaaattt tgcaatgaat
     4261 tatagagtcg ttctctatca gccattgcaa cattcaaatt gttcattttt agtttaaaaa
     4321 aaactttatc aatattattt gctctaaatg gaccaaataa gtaatatctg gttttaaaca
     4381 tgtatagaag aaacatctca ttaaaatcac cagtaaagaa atggaaatct ttagtttttt
     4441 taatattatc agttagtaac tttttataat cataaaaaaa ataagtagtt ctatctgagc
     4501 gatactcttt tataatactg aattcggaat taaaaaccac gataggtaac tttaaacaaa
     4561 catgaagaga ctttaataag acttctttat ctgaaaacac ttttgtttct ctctttcctt
     4621 tctacatcat taaatagtat tttatacgtt atctaaaata ctattcctct gtcaaaataa
     4681 gctaacttac ttcattataa aatatccact tatcgctttc atgtatattc caccgtaaaa
     4741 agtacatttt atcaccgaga actcatcatt tttcatcatg atgaatacta tttacatata
     4801 tctagttcaa agacaataat tattatgatc cgttattatc taagcactat acagataata
     4861 ggaatttttc gtttctaaat attccaattt gggaatccaa taaaaatttt cgttcaaata
     4921 cgtgtctatt cgaactcttt tctagttgtt aattatgaag ggggaagaaa taaaaaatac
     4981 aatctataca cgaataagaa tattatccaa aaggaggttt tagaaaagat ttattatacg
     5041 taaaaagctc aagacatttt cttagccata aggacatgtt tgatatttaa gaagatatat
     5101 gaattttctt ttctaagtat aaagataact atccattatc aactaaaaaa acatttttga
     5161 aaataaacat cagcagtcct atcaatgcat gtgagcttat agaagttatt cgcagcgcag
     5221 cataattgca ttagcttctt ttcgtgtttt ttgcctcttt tccctttaaa tcttaatgga
     5281 ggaggtgtgc aatatgtcag ataacgacga acgcgtaaat gcccagttga ttcgattctt
     5341 tgaaaaggtc attttgaaca ccgctaatac ttattataaa aaacaagaag aaattcaaaa
     5401 acatgaacaa cttgatcaac tcccaccata tttgtttact aaagatacta taaaatttaa
     5461 acaaccgatt aatgttttaa atgtgactct ccttgttgaa gatataaaat tagcaaaaat
     5521 ccttcctttg ttgaaagaac ctgaacaaat ttttcttatc gaaaaattta tctttgataa
     5581 aactgataaa gaaattggaa aggttctggg gattacaaga cagggtgcga caaatttaaa
     5641 acatcgactt tacaaaaaaa ttcaaaaaat gctggtttaa tatctgcata ataactaatt
     5701 ttgtatgctt ctttgctcgt tctcctcctt cccaaagaaa tagattataa gtccatggga
     5761 agaaggtaga tttatgaatt ctttaaaaga agatttcatt ttagcaaagg ctggaaatga
     5821 agaagctgtt gaggcaattc tcaaaagatt tagctctttg attcataagc aatcctggag
     5881 aactggaaaa tatgatcaag attgttatca agaatgtatg cttgcaatat atctagctat
     5941 ctcgaaattc gaaattaaag aataactcaa ctaagggagt ctgtgacata agtaaaaaga
     6001 tagtttcaaa tttcgaacaa taaatttgaa actatcttct ttataattat aaagtatcga
     6061 ttttacagcg ttctataatg attcatagaa tctaaaacga atgattttaa ttatcaacgt
     6121 tttttaattc gtttttagct taaaaataac ttttgtcaca gtctcactcc taaaattact
     6181 ttatttacgt atgaagtggg caggaggtcg aatccagaac tgggctatgg tacttcaacg
     6241 aggacttctc ctctagaatt gaaaagcatg cgatttacct tccgtaacga cggtagattt
     6301 attcaactat gtgtccaatc aaaatttgtc tgatttcaaa aatagtcaag agaattttgg
     6361 ttgttaaaaa ctcttatcta tcaacttcca cagtttagtt gacaaaccct gatactaagt
     6421 tgggctatga tacacttttc ttgactatca gttacgttac cggaaggaat gaactctatt
     6481 gagaaaaaca tatgatcgta aatttaaact aaagatttca caggatatcc ttgagaaaaa
     6541 agtaactacc aagaaaattg ctgaagaata caatatctct cgtccaacaa tttcccgatg
     6601 ggtttcagaa tatcgtcgat acgggaaaaa tgcttttgct ggacaaggaa aaagattacc
     6661 tgataaagca gatttctaca ttcttgaaca ggaaaataaa cgactaaaag aggaagacga
     6721 gtgttctacg aacacaaaga tcgatacgga tctccaggga tcactcaaaa actttacgat
     6781 gaaggaatcg aaatcaataa acgtgttgta gctgttctta tgcgtgaaat gaatttatgc
     6841 acaagaggat ttcatcgacg caaatcttct catggtagag aaaaaacaat caaagaaatg
     6901 gtaaaaaaat gttgaatcga taatttgaac agaaccaaat tgatgctgtt tgggtgatgg
     6961 atattactta cattccatgt aatgacggtc gcctctattt gtccacctat attgacttag
     7021 caactcgtat tctacgttac tatatggtag attcacacat gaaaaaagag attgtcgtcc
     7081 gtccattaca aatttattaa ggagaattgc ctacggtaat tcattccgat agaggtagcc
     7141 agcatcggtc ttttatttat caagagctgt tgaacgacca ccacatcaca cattcagtga
     7201 gccaaactgg gactcctgtt gacaatgcag taattgaagc gtttcatcga tcaatcaaat
     7261 gagaattaat cgactccaac aaatataaaa gtaaaataga gatgaaggtg ttaatccaag
     7321 attatttaga agactactac ccaaacaaaa gaatccatac aaaatttatg atgactcctt
     7381 gacaatttga agcgaagaaa ataagggatc tataatatct agtgttggtg acgccttctg
     7441 ttgccaatac tttttctttt ttctaataaa cgcaaacaga caccttaaaa tcctttagaa
     7501 taaagtcgac gaaaaccatt caaaggagtg tttcaagatg tcctattcag agtttaccaa
     7561 agagttacta gatattctag acctaaatct tatctttcat gaagatgcct ttcgcaaaga
     7621 gcgaatcaat gacgaaacat gttttgtttt tgatggcaca ttgacctatc aaccggagga
     7681 atgctttcat cgccactatc aaaatgaaca aacgatcatt aaatagggct ggaaaaaagt
     7741 atccatctta ttgaatgatg tcagtaacta caagaccatt ctgcggatta acaaacagcg
     7801 cttcaaatgt aaacactgtg gaaagacttt tttagcagaa gattcagtta gtgatcgtcg
     7861 ctgctccatt gctcgcagag tcaaacaagc cattcttgag ctattaagcg aacctctttc
     7921 tatgtctttg atcgcacgta tgaagcacat ttctccgacc actgtcattc gaatacttcg
     7981 cagccttcgg cctaaaacca tttctttgag ccagctgtta ccagaagtgg tgtgtttcga
     8041 tgaattcaaa tcagtgaaaa atgtctcggg agccatgagc tttgtcatga tggacggcaa
     8101 gacccatcaa ctgattgata ttgtggaaaa tcggcaactc aatcctttac gcgactattt
     8161 cttgcgctac cctataaaag tccgtgaaca agtgcgtcta gtggtttcag atttctatac
     8221 accctatcgc acattggtga aagagtgttt tccgaatgcc cgaatcgtcg ccgatcgctt
     8281 tcatattagc caacacattg gtcgggcttt taccaaccac cgaattcaag taatgaagac
     8341 ctttaaaaaa ggggatcgtc ggtcgaagca cttaaagaaa tactggaaac tacttcaaaa
     8401 gaatgcgtgg gaattgaaag gtcagcatcg ctactggcgt ccgtcatttc gagatcatct
     8461 gacagaagca gagatagtcg atcggctatt atcttatgat gattccttaa agcgaggcta
     8521 cgaagtctac caggattttc tttccgttat cagaagacaa gatgttcctg aattcgtcgc
     8581 gctcctaaaa gaagactaca aggagttgcc agagcactac caaccagtgt tcacgacctt
     8641 caagaaatac caaacagaaa tcaaacgagc gttgcgcgtt ccctattcca acggccctat
     8701 agaatgtttg aataaccata tcaaagtact aaaacgaatt gcctatggct atagaaactt
     8761 tcaaaactac cgagaacgaa tctttttata tcgaggaaaa tattttaaga aaactaagaa
     8821 tactacccaa ctgactaaag ccagaacaac aacacggcta gacaagatag ctgtctagcc
     8881 gtgtgagtat ctcgattatt ctattgaatt tcttcaccaa cactcattga cgaagagcca
     8941 aaataagtaa tctaaccgac tgttaactaa accattgcag gtcccgttaa agatacttgt
     9001 gcctgttgtc accgcttctt tttcttgtcc acaagcgctt aacacactta ataaagcccc
     9061 tgctattatt aataataata tctttttctt attaaaaacc tcctcatcta tttccagatt
     9121 agtaaatgta gtaatctgtg ctttcttttt tctcaatact aataactaac ttatggggta
     9181 aacaaatact cgtttgaccg ggttttgaga tccatccagt tctaaccgcg atttgatcag
     9241 gactattatc ttctttaact cgaattcttc catctttaat ctcaacaata ttatattgtc
     9301 ccttcgcagg atgataattc acgagtttat gattgatctt atccaaatcg aagcgatcca
     9361 tttccttgcc atcaattcga acgatggcat atttgatagc atcttcagtt gtttcattct
     9421 ccttttctat agagaataca aataagggaa taaaagaggt aataatcaaa atgccaataa
     9481 ttatgacatc gcctatcttg acaccagctt ttcttaaaaa tttcattaac gatatctggc
     9541 taaacatatt agactttttc catggaaatg gtgaaatttt tcggaaatta tctgcattaa
     9601 gagaatcagt taaacaaccc tcaagttgtt ccttaataca ttgttgatcc atcttcatac
     9661 caataaaaac tagtttatta atgcgttcac caaacaacgg atgccaggct tccttcaatt
     9721 cgggattttt ttgtataatc atttgacact caattttagg taaactagct acccaataat
     9781 ttaatgattc aatactttta tagctacccg atgtttcaaa gatcgtcgaa aaatcactat
     9841 gttgggcaaa ccaaagaaac ccttttgctc ttgtaatatt gccaggaaaa tttttatccg
     9901 taagatgggc aaatttttgt gcgtcaagga gtaaccgatt tcgatagata aaacttgaga
     9961 taccatattc ttctgtttca ggaatatgat tttgatagcc aagctctaac tctttgaacc
    10021 agcccactga ataggaagtc tcatctatat taaaaagatt tgtatttaga attttcgata
    10081 agtctacttc acctttaatt gtcctaatta ttttggcatt ggactgtaac ttagcaatta
    10141 gaccgttaat tttgtaaatc gtcttggttg acactaagtc actattattt aaaagtaata
    10201 tgttacaaca ctcgaattga tcaattaaca agccacgaat atcctgttct tcttcaacta
    10261 cattaccttc agcttttctt tcctgaatct tttcacccgt ttcgaaatta tcccaaaaac
    10321 gttttacatc cactactata ataatggcac caatctctaa tcgttgggtc aaatcaatgt
    10381 ctaaagggtt tttagctaat ataattgctt gaataattgg aattggtttg ctgattcctg
    10441 aagcttcaat aacaatttga ttcaagtcag aaaattgcac taattgatct agttcaacaa
    10501 tcaaatcttc gcgtaatgta caacaaatgc atccattcgg catcgaaaca agtttttctc
    10561 cagttctttt aaaacctttc ttgtcgacta ataccccatc aacatttact tcccctatgt
    10621 catttacaat gacagcaatt tcctttccgt gatcaccgta cagaatatga tttagaagta
    10681 tcgtttttcc tgaaccaaga tagccagtta agattgtaat aggtattttt ttcataggga
    10741 acctctttct ataccttgaa tttacacttt aagtgcaaca cataaaaatg agtcataatc
    10801 gatatccaac tagtataagt ttgtcgagaa cactcctaga aggaaaataa ttatgagcca
    10861 gtatcgtcat cttactatac tcgaacgaga aagaatcttt ctgcttcacg aagaaggatt
    10921 ttctattagt ataattggca acgacattca gcatagcccg tttactattt tacatgaact
    10981 gagaagaaac aagaaaaaaa ctattcacct tccaaagcgg aaaaaaatta tgcttatcga
    11041 agaaaaaaat gcggacgtta tcacattcta aatgatccgt gagtttggtc aattgttcgt
    11101 catttatttg tcgattttca gtggtcgcca gaggaaatct cgaatcgttt gaagcatgaa
    11161 catatggact tttcaattag ttttatcacg atctaacgtg caatttgcaa tgatcttttc
    11221 gatacagaac ccctctcaca cggcaataaa ggcctgatcc gttcattgca ccatcgagga
    11281 aaaacaagac atacaaaaaa tcatgtcgag tctcgtggaa agatttgcct tactcattcg
    11341 atccatggcc gtccaaaatc cgcagatgat agaagtgatt ttggtcattg gaaagccgat
    11401 attgtagctg gcaaaactgg tggtacttgt ttagtgactt tgacagacag aaaatcatgc
    11461 ttccttttag caggaaagaa ggtccccaga aagaaagccg cttatgtgag cgagaaaatg
    11521 attgaacttt taagcagtat tccaaatgag gcggtcaaaa cgatcactcc agatagagga
    11581 aaagaattct ccaatcattt cgcagttaca gaagcattga ataatatgcc cttttatttt
    11641 ccagatcctc atgcccttgg caacaatgaa caaattaaaa tacgaataga ctcattcgag
    11701 aatacttgcc aaaatcgaaa gagatagcta cggtagataa ctcaacgata aatcagatga
    11761 ttgaaaaact gaataacaga cctagaaagt gtttagattg gagaacacct tatgaagtct
    11821 tctttgataa agtgttgcgc ttgatttgac aattcaagac ataataaaaa gcagatcaaa
    11881 tccaaataag ggaaaaatga tctactctgg tattatcaat taattggcca agctattatg
    11941 ccgttttgct aaacgtctac ttcttccagt attgaccttg ataaaacaac aatcaggatc
    12001 ccgttttaat actctatgaa ctgtgtcaat actaacatgt aaagttgcag caatatcagc
    12061 taatgtactc ccatttaatc tcatttgctt tgcaaatcga taacgccgat aagcatcaat
    12121 attcttggaa tccatagaca ataatacttt tcttaccatg tatttcctcc aaaaattgaa
    12181 ttttaatgag ccatcatttc ttgagcaatt tcatgtttag acaatgaagc tggatagaaa
    12241 gtaggccaat tatgcaattc attatagagt ttttcttgac tttctccacc aaagaaaata
    12301 tggaaatgct ctgccttact tggtgcaatt ccatgatcac taaattgaat atatttaaaa
    12361 cgaccagcac ctgcatcatc agtttcaaaa ttaaaacgaa caccacggtt tcctttttca
    12421 taattcaaaa ttttgtatcc aacatatttg tatgtgtatt gatgatgttc accgttgact
    12481 acaaaatcaa tggtgttgtc tttaatgttg atagtatcaa tatctgtctt atagccagta
    12541 gtataataat ctttgtactc ggcagctgtc atgtctttat tgatttttgc tttgtaatcg
    12601 aaaacttcat ccaaaattcc atcttttaac aacggataaa cagattgcca ttcgcctgta
    12661 taatcactca attcgcgatc cttcacatcg gcatctttaa agtacccatt agcaactgtc
    12721 ttttcatcct tcgcttcttt ttctggaact acctcttcac cagcaaccat agttgttttt
    12781 tctaacgctt ttagattgtc ttccattact gaaacataat cttcaccatt atccatttgt
    12841 tcttttgtaa gactttcaag aggattcagt acttccaatt ttatcccagc ttcgttggcc
    12901 aaagtttttg caattttatc attagcattc ttttcaaagt aaatataatt gatcttattt
    12961 tttttaacat actcttttaa ttcagctaat cgaccagaag atggctcttc ttcaggactt
    13021 agtccagcaa taggaacttg tttcaatcca taatccaaag ctaaatatcc aaaggctgcg
    13081 tgttgcgtca caaaactttt ttgtttcgct tctttaagac tagtggtata gtcagcatcc
    13141 aaccgtttca gtttagtcaa atacttttca gcatttgtct caaatacttt tgcttttttc
    13201 ggatacaatt taactaactg atccttgata tttgaaactt ccttaattgc cattttgggt
    13261 gaaacccacg tatgaggatc tagttcatga tggtgatctt cttctccgtg atcatgatct
    13321 tcttcttcac tacctggcaa taaaatcatg tcttttgttc cttctactac attaggccca
    13381 gctttcttcc aactttcttt agcttttggt acccatgatt ccatgttttc gttatgatat
    13441 acaaaaacat cagcatcatg agcttttgcc atatccttag ctgagggctc atagtcatga
    13501 ggttctgatc ccgctggaac cattaaatcc acgttcgctt catcgccgac gatgtttttg
    13561 gtgaaatcat acattggata gaatgttgtt acaatttcta attttccatc tttatcattc
    13621 gatacttttg catcattagt gctggagcaa gctcccaata atcctagagc tgctatccca
    13681 ataagtccta cactgatttt cttcattctt tttccttcct taattatgct aatttcttca
    13741 ttttatttcg tttatgttct ttgataattt ttaatgccct ttttctagtt ttaatattcg
    13801 ggcttttaag tcgtcgatat gcactagata tatccgtttt ctccattaga aattaccccc
    13861 aatcactact tatcattatt tgctttccgc cagtaattta tcaaaactct gtttgatcct
    13921 ttcttcatcc aacatttttc cgattagaat aatagtggtt tggtcttttt gtcgagtggt
    13981 gtattgaaaa ttaacctgtt cgtttacctc ttgcatggca actataaaat cctgctcttg
    14041 taaagcaatc aatcctttaa aacgatacag gctcccttgg ttggttaaaa tcagccagtc
    14101 aatccattgg agtaaaaaat ctttttttat gtcttgatct gaagttaaga gaattgattt
    14161 aaactcatgt acttgatggt gttgatgata gtatttgcca ttatagtgat tttctgatga
    14221 atcactttca aaaactaagg gatgattaaa ttttttcaaa ttataaaaat cctttttttg
    14281 aatcgtttca ctatgtgtga acattcgaat atcggataaa gggttaatac ttttgatttc
    14341 tctctgtaat ttttttagtg cagataaatt caagttttgt tttctagaca caaaaatgcg
    14401 atctgccatg acaagttgct taagggcttg tggttctctt tcaataagtt caaaactgtc
    14461 actatcaata acagtcaata aactatcgat atatactctt cctcttagtt gaggcgctgt
    14521 taaaagtgtt tgaatgattg gttggggatc tgctatacct gtagtttcaa tgatgatttg
    14581 tgaaattggt tgttgatgtt cttctattgt ttccagtaaa gaaatcaata ttgctaataa
    14641 atcactgcga agactgcaac acatacatcc attatttagt tgtaacaccc gttcctcgct
    14701 atgaactaat aatttgtgat caatcccaac ttcaccaaac tcattttcaa ttataataat
    14761 ttcttccctg gggtagggtg agacttgcaa ggcttgattg attagcgttg tttttccagc
    14821 ccctaaaaac ccagaaacga ttgtaactgg tatgcccatt ttgtcacctt tttaccactt
    14881 agctatttca cttctttaaa aattgcccgt ctgcgcaatt ttggcgaata cttttttaat
    14941 tctaaccgtt cagggttatt ccgtttattt ttgctggtga gatacagacg ttctccagtt
    15001 tcgacacatt ccaaaataat attttgacgc ataaatcttc ctccttcaat acttatcgat
    15061 cttcaagata tgcttgaatt tgttgtgcat cttcaaaagt taactcgcca gattccaatt
    15121 gtgaaactaa attttctttc atatcaacag gtatctccca ataaccatcg atacattgaa
    15181 ataactcctc gtcatactct tcaaaaattg gatagattct taaaaaatca tcaaatgata
    15241 agccttggtc ctcatagtaa tctttgacta atccattttc aattgcctgt tgggtcggta
    15301 aacccgtttc agtaataatt cctctctcta ccatcgaacg ataaaaagtg catttatcta
    15361 gcgattgctg ggtcgtttca agataatact ttaatccttc ttcaactaaa ggtgggtaaa
    15421 taatttccat tttgattccc tcctaaaagt tatatattaa actattttga agctccttta
    15481 attactttct ttccattata gtaccctttt aaagatacat gatggctacg tctataatca
    15541 ccattcgctt cgtcaaatga aatttctggt tttactacag tttgatgagt tcttcttaac
    15601 cgtttcttcg ccttagatgt ttttcttgct ggtactgcca tgatatttcc tccttttacc
    15661 aacttgcttt ttttactcca ggaatcaacc cttgatgggc aagttctcta aacttaatac
    15721 gggacatacc gaactttcgc atataccctc ttggacgacc atccgtctga tcacgaagcc
    15781 tcagacgatt cggatttgaa tctcttggca atttagcgag agcggtgcga tctcctgcgg
    15841 cttttaattc ctgacgttta tctgcatact tttcaatcat cgccatttgt ttctttgctt
    15901 tagcaatttt cgactttttt gccatatacc tttatactcc ttttattttg atttagacac
    15961 ctcaaatcgt aatcgttacg ttttgacact gatactttac caacaagagt tgctcttgtc
    16021 aacaataaat aacatgaaat aaaactcata tgaatagata actatttgaa aacgaattta
    16081 tcggtacata gagataatca aatcatttaa aagaatatgc agtttaggct ctgaaaaata
    16141 agaattcact tttattagtc caatcaaaca caaataccgt tttaagtagc ttttcctcta
    16201 cttaaaacgg caatatgaaa gaaactataa ctttttattc aatcttttat gtttaactgg
    16261 tttatcgcca attttttaaa actttgatta ttccttatat tataagaata gacattaagt
    16321 gaactaattc aatcatactg attctttcgt gtattcaaaa ttagatgctg tttaataaaa
    16381 ttattctttt tgaaaaagaa ttataactat atcccaaaaa ttgtaggtta atttaaagcc
    16441 tatttcatca tcgtgattga gcttcttttt catagacgaa tttctcagat agtttagttt
    16501 tctttatgag atttttgtat atttctgacc ttcgatacaa ctcattatga gtaccttgat
    16561 caactagttt tccttgatcc atcactacaa ttttatttac cttttcaata gacttcaagc
    16621 gatgcgatat aattagtact gtcttatctt gtattaaacg atttaaactt tcctgaatac
    16681 gtttttcatt ttccgcatct aaactggcag taatttcatc taaaattaaa attggcgcat
    16741 ttttcaaaaa agcccgtgct atggataggc gttgtcgctc tcctccagaa agtgaacttc
    16801 cgttttctcc gaccaatgta tgataaccgt caggtagttt ttcaataaag tcttcgcagt
    16861 tagctaaacg agctgctttt ttcacttcct catctgacgc ttgactattt ccaatacgta
    16921 tattttccaa aatacttgta ttaaacaaag ttacctcttg aaaaacaata gcgattttgc
    16981 taaacaaaga agctggagat acacgtttaa tatcataccc atcaattaaa atacagcctt
    17041 catcataatc atacagtcta gacactaatt tcaagatact agttttcccg gaaccactag
    17101 cacctactaa cgcagtcact tctccttgtt tagccgtaaa agagatatga tctaaaattg
    17161 gggtattgtt atcatatgag aaagagacat ctcgcaactc aatgtcaaaa gatttcagag
    17221 gactatctga tccctcttgg atactggttt ccttcatagc tctaattcgc tgtattttag
    17281 gagctaaata aaatatttca agcactgctt ctttcataga atcgaaggaa tcctttattt
    17341 ttattgcggc tagtaagtaa ccaacgacgt agagtatagt cacttcaccc gttaacagta
    17401 agtgaacacc tactagaatg actactgcta aactaacata tgagaaaata gaggataagg
    17461 ccatgactga aaaagtactt aattctacct ttaaatgaat cctttcactc tcttccattt
    17521 ttttgaaaag tctgtcttga acttttttag atagattaaa gctattaatc tcttgctgca
    17581 attctatagt ttcttgaaat tcttccgagt tttctctcaa agtatcgtaa tatttagtat
    17641 tggctttcgt ttgggatttc tttgataaca agattaagac aaaactaaat aacgttggca
    17701 aaataacagc taatcccatt ttgacattac caaccaaaag catcactgaa ataaaaggga
    17761 aaaacagagc cataccgccg gctttaggta ttgcatgact catcgcatgc tcaatacctt
    17821 caacgtcaga catgatagtt tgagataaat ctgataaatt atgttttgaa aaataggata
    17881 atggtaaatt tgacaaatcg tctgcaattt gcactcttaa atgcgcactt tctttataag
    17941 tactattata taagttttca tactccctat ctaacaacca atacatgaaa agcaaagtaa
    18001 caaacgatac ggcaaaataa agccaacggg gctttgtatt ttctaaaact aattcgtcta
    18061 tcagcatcat tataatgaat gctggtacca tattaatgca gtatacaaaa aaactagaaa
    18121 tagaagcttt agttaaagct tctgcgccct gatcacttaa agcaaatttg tttttataaa
    18181 aacttttcat tgtcaagcct ccaatcattt gcattctcat aattagtgac caattgttta
    18241 tacacttgat gtttagccat taattcatta tgatttccac gttctactat tttcccttct
    18301 gacaagacaa gaatttcatc tacagattta atgctggata atcgatgagc tatcataata
    18361 actgtttttt ctttcatcag atgttgaaaa gccttttgta gttcatactc attatctgga
    18421 tcaatagatg cactggcttc atccataata acaatctttg catcttttaa aattgctctg
    18481 gcaatagcta tacgttgttt ttctcctcct gacagataaa ctcctttact accaattaag
    18541 gtatgctctc tttcgggaaa tttttctaaa atcgattcgc aaccggctaa tcttagcgcg
    18601 tcaaatactt tacttgctgg cgcttgagga tttgctatag aaacgttatc gtaaatactc
    18661 tgattgaata gtttcgtatc ttgaaagaca aaagcaattg tgttaataat tgtgtctttt
    18721 gaatagtgat ttaaaggctt accaccaatt ttaatctgtc ccttatccac attgtagaaa
    18781 cctgaaatta gtttagctat cgtactcttt ccgcttcctg attttcctac taatgcataa
    18841 attctttttt gttctaaaac taagttgaaa ttttctaaaa cacactcttc tttgtatgaa
    18901 aagctgacat tttcaaatgc aatgtcacaa tttttaatct gtgtctcgtt cccatatttt
    18961 agttggtctt gcttcatttc taaataaagg gagtctagtg tttcgactgc atatcttgct
    19021 tcaaacaagt gcatacttac atacattatt ctcatgaaag aaacaaaaat aattccactt
    19081 aaaaaaaaca acatcaacaa gtcaacagct aatatcttgg gagtggtaag agaagttata
    19141 aaaaaggtta taggaactat caaaatgcaa actatgccaa aaaatagcca ttgaaaaaga
    19201 acaaaaggtg ttttcccact caaagaatac ttatatgcaa acttagcata atcgttaatt
    19261 gctttattca aggctttaaa tgatgttact ttagcaccaa atatctttat aacttgaatt
    19321 ccgcggatat attctaccgt ttcagagctt aacacatcta acgattcttg atatttttga
    19381 ataaaatttg tattctctgt cattttcttt aacaagaata caccaattac taacatggcg
    19441 attagaacca atccaatttt aaagttaatg aaaaagccta aggcaactga taaaacaggt
    19501 gttactatgg cttgagcact atctggaatc aagtgagcaa ttgccatatg tgttttagcc
    19561 gcgttgtcat caattgtctt tcgaatatat cccgaagaat ttaaatcaaa aaaacgaaaa
    19621 cttgcttctg ttaaaccgct aattcccttt tttctcaaat tagtctctaa gcgaaaccca
    19681 aggatatgag aaaataagcc agaaaaaata taaaataaag ctcctaaagt taaccaagca
    19741 acagtttgaa ccgcataact cttagctaaa tattcatctc ctacaataat tagagactga
    19801 agaaatttgt aaatactgta atatccatag actgttaata cgacagaaaa atcagaaaat
    19861 aaagtggcta accatccgta gtatttcatt ttagggacat aactaaataa ttttttgtat
    19921 actttcattt tttcctcccc cgcattcaat ttcttctctt attcgtttac aactgttaat
    19981 gctttccgta atagcgtaaa ttagagatgt acaaacagat aaaaggtaaa ctacgttcta
    20041 ttttttgtat ttttttaaga cttttcttcc aatcaataca ccaatcatag ataaacttgc
    20101 tacaactaac actgaaatga ttccccatat acctaccgca tagtggatca ttgcatccat
    20161 tgttgcagca gattgtcctt tatctatcca atcttgacga aaacgatctg taaagaacca
    20221 tataggaatc cactgtccgg cagcactgcc taattgcatt agagcccaag acgcaacttg
    20281 aggaattgct cgatcatatc caaatacaga tagaataata tcagctaaga tcgccatact
    20341 aatcgaactg ataaaccaag gtaagtacgc cccgcccatt aacgtaaaaa ataacaataa
    20401 aacaattgtg taaatagtaa aaattccttt tctagggtag ttatagctaa tataaacaaa
    20461 tatgattcca gataataatc caaaaatccc gggggatatg ctgtgaccaa acgccccacc
    20521 aatactacta accattattg aaaaaaagta aattactagg gaaataactg tagctaaacc
    20581 aataaaaata taatcttttg ttttcatata tattctcctc aattctattt cgttatgtca
    20641 ttaaaaatct gttctatcaa agttgaatct tctactctaa gttttatatc tttttttatt
    20701 ccaccatttt ttaaataaat aattctattg catgttttat aaagaaattc cggatcatga
    20761 gtagctataa ctactgcacc cttttctttt aagtctttaa taaggtcagc aatcctttcc
    20821 atattaatat aatccaaccc acttgtaggt tcatcaaata gaaataaatt agtttttgac
    20881 aatgaagcta tagaaaccaa taatctttgt ttttctccac cggataaatc aaacggtaat
    20941 tgatctttca ttttataaag ttccattttt tttaaaattt tttcttgctg atctttacta
    21001 actatttcat tccccagggc taactcgctc ctcactgatt ctgtgaaaaa ttggtaatcc
    21061 atttcttgca ttactaaaaa cggcgatgac aaattttttt tattttcgat tatctttcct
    21121 ttggtagggc gaataatctt catgagtatt cttagtaagg ttgttttccc cacaccgttt
    21181 ttccctacca aaccaataac atctccaact ttgacatcaa aatttatgtc ttttagaata
    21241 ttttcataag taatttcttc aacttttaat acatctgcac ttaccttttc gatataggga
    21301 acttcaatcg aactattaaa aatgtcaaat ttccttaatg cgttattgtt ttcagttttt
    21361 aacaactgaa tagttgtagt ttttacagtt ctatttttaa caaatagaac ttgatcaatt
    21421 aattctttca aatagtaaaa acgatgttca gctataacaa tagtaaatcc tttatttttt
    21481 aaatcacaaa gtaagtcagc aagtttctta gtcgctttga agtccaaatt tgaagacggt
    21541 tcatccaata taattaattt agagtttagc gataaactac atccaataga aattttctgc
    21601 cgttcaccac ttgatagcga aaaaatgttt ttatctaata tatttgaaag atctaataat
    21661 tcagctaatt cattgattct tttatccata tgctcttttg taaatccaaa gttttccatc
    21721 gaaaatacta actcactcgt tgtatttgtt gtaaagaatt gtcctctagg atcttgactt
    21781 acgtagccaa tattttttga tatactttct tgtgttaagg attgtagcga gttattttca
    21841 aataaaattt ccccaaccaa ttttccgtca taaagtcttg gaataagtcc attcattaac
    21901 tttaatagtg tggatttccc actcccagaa gaaccagtaa tcaatgtaag actgcctttt
    21961 tttattgaaa aattaaggtc aacaagcgct tgttccttaa catccttata tgaaaaagaa
    22021 acattgttaa attcaaacaa ttctttcacc tcaaatcaaa atacttaaga aaaaaatagt
    22081 aacggaaaac aggatgatat agtctaataa gctcatgttt gttttatagt aagaacttct
    22141 tttcacagga gattctatcc cctttgttat tgcattagcg gtcatttctt cagcaatcat
    22201 agttgcacgt ataagaacgg gaactaacag aaattccaaa tattgcagag gctgaaaaaa
    22261 agacaagcga atgtttctca tttttaaaga ttctttaatc attcttattt cagaacgata
    22321 agtcggaaag aatctgatca ctactgtaat acttaacatt atttttttcg gtaaatgtat
    22381 tttttctaaa gaggataata attcattgct agtatagaca ctaaccaaca accatgagat
    22441 cattacgacc ggcaacattc gaaatacaat atataaggat gtcgcaaaaa atatagtaaa
    22501 aaactctatc ttgatagcat caataatttt cattactaaa aacagaactg aataaatcaa
    22561 gatgaaataa atacatgtct ttattttacg aaacatcaat actaaaactg tagcaataat
    22621 gaaacacccc acttgaaata tatttccctt tcctaaaaaa ataatgaccg gaaaaaaaat
    22681 atttaaagta atcaaggtta acggattgat ttttgacact ctttcacctc caaaaaataa
    22741 gatcgcactg ttatcttaag ctgataaaaa tgcaccgtta ttcagataac agtgcgatct
    22801 tatcaaaaag cataactgag aacaattctc aatgcaagct ttttttaatt aactcagtaa
    22861 ttcaatccac ccaggaacag aaaattgata caatcttctg gttaatagca tagtcttttc
    22921 ttttgaatag ctatgcttca atatatcata gaatgtagcg tagaatgagt aaatgtagaa
    22981 gtggatttct tcttcattaa agactaaatt ttgattgatc attccgatag tctttaactt
    23041 atctagacaa cagactgtct ttttcgtcac aaatgatgta attttttcaa caatatcttg
    23101 atatttggtt ccagctgatt ggaataaaat caaatcgaat atacgtttat tctcaaaaat
    23161 ataattaata aatggatcta attcattgaa gttctctctc caacttttct ctatatctat
    23221 attttctaag tttaagaggt atttggtaaa ctcttcatta tacatattaa taagcgcatt
    23281 aatatgcggc tgaataattt cttcaaataa cgcttcctta gaagtaaaat gtttataaaa
    23341 agcacccgta gttacgcctg cggtcaggca cagttgcctt atcttagttt gctgaaatcc
    23401 ttctttttca aaatggctta gtccactttc tattaacttt tggtgagtta taatataatc
    23461 tctggtttct atgggtactt cctcctaata agatgaatta actttattat cttacacagg
    23521 acttgaagtt tcaatcgtta acgtttatta ctgaactcat tccttagagc taaactaatg
    23581 agaaagagca gaataactga tacaaaaagc aatcctacaa gaattaatag actcatctaa
    23641 atcattcacc ttttctaaaa taatccgaga aagatttaaa aaaattatat ccagctatta
    23701 ggataagtcc tatcataatc actccaatag ccattttctt atcctcccta aaaataaagt
    23761 cttttactta aaaccttttt tgtaatcgtc aataaatttt ttccctttat cagttaattg
    23821 aaatcctata tctaaatagt cctcatatct aagtttagcc agcatctcac ttacagatgc
    23881 tggactaaca cttagaactt ttgctaaatc attatttttt aatttctttt ttctttttga
    23941 taattcctga attgctagta aataaattag tatagtagtc gttttaattt gtaccatacc
    24001 taccctccaa attatttcga aaggccttca gcaattttat ccaggttcca tttcatcatc
    24061 gcatagtaac tatcaccatc ttgtcctttt tctgcaattg aatcagtaaa aatcgttgaa
    24121 tagataggaa tattggtatc ttttgatact gttttcattg gtctatcatc cacactgctt
    24181 tctacgaata aggagggaac ttttgttgtg cgtaattttt caactaagtg ttttatttga
    24241 tctggtgttc cttcttcttc agtgttgatt tcccaaatgt aagcagaagg cacattatac
    24301 gctttcgaga aatatttaaa gcatccttca cttgtaacaa tcattttttt atcattcgga
    24361 attgaagcaa atttagattt agcttctttg tctagagaat ccaatttttc aatatactta
    24421 tctagatttt ctttatagaa ttttttatta tcaggatctt tttccgctaa ctgtttttca
    24481 atatttttag cgtaaataat accgttttct aaatttaacc aagcatgagg atcttccttc
    24541 cctttctcac tctgaccctc taagtaaata acatctatgc catcacttgc tgcaaaatag
    24601 tctttgtttt cctctttgtt cgcattttta actaattttg taaaccaagc atttcctcca
    24661 gtttccaagt taacaccgtt ataaaaaatc aaatctgctt ttgaagtttt ttgaacatct
    24721 tcaggcaaag gttcatattc gtggggatct ttcccaatag gtacgatact gtgtaaatca
    24781 attttatctt ttgctatatt ttcggtaata tctgctaaaa tcgaattagt aactactact
    24841 tttaattttt cgttcgaagc cactttatct ttgtctttac tatttattga acatgccccg
    24901 aatataaaaa gagcaatcaa aatccccaga cttcccagta ctgctttcca ttttctcatc
    24961 ataacgatct ctccttcttt cttaaaaatt tttgttttgg tgagattaga aaactaatta
    25021 caaataagaa tgccgctgtt aaaacaatac ttgatcctgc tgctatgtta aaactataac
    25081 caataaacaa tccaagcaat gatgaacttg ctccgactgt tgcagaaata aatatcatag
    25141 ttttcaagct atttgcatat aaatatgctg tagctgctgg tgtaattaac attgcaacaa
    25201 ttaatatagt tcccacactt tgcatagctg ttactgctac taaagttaat agaaccatca
    25261 atagataatg ataaaaactg acattcatcc ctattgcttt tgccattaat ggatcaaaag
    25321 aagtgattaa taattcttta aaaaatagaa ctactataac caacactaaa accgaaacgc
    25381 caattgtaat ccacttatca atatcttgta cagctaaaat atttccaaat aaaatatgaa
    25441 ataaatccgt tgagctattt gcaactccta taaggataac tcctagtgct aaaaaagaac
    25501 tgaaagtaat accaattgct gtatctcctt ttattacact attgcttttt ataaaagtaa
    25561 taatgataga agctaataga ccaaatacaa tcgcaccaat aaaaaaatta atacctaaaa
    25621 tataagaaat tgctactcct ggcaaaacag catgagaaat agcatcaccc ataagagaca
    25681 ttcctcttaa aataataaaa caacctactg ttccagaaac aatccctata actacagatg
    25741 taattagagc attctgtaaa aagtgaaagt catacagacc attaataaaa ttttgaagca
    25801 tattattccc cctctccaat aaatatagta tctccatagg ctttttttaa attttcttca
    25861 ttaaatacag attctacact tccgtgagca attagatttc gattcaaaag aataatatta
    25921 tcaaaatact tctttacctt gcttaaatca tgatgcacaa ttagcactgt ttttccctga
    25981 tgtttcaatt tttttaacgt atccataatt attcgttcac taactaagtc tatccctaca
    26041 aatggttcat ctaaaaatat gaaatccgca ttttgagcaa gacatcttgc taaaagtaca
    26101 cgttgaaatt gtcctccaga taattctcct atttgatgat tagaatattt tcccatatcg
    26161 accttttcca aagcctttga aactctttgc cattctgcat ttttaattct ttgaaaaact
    26221 ttcattccgg catatgttcc catagataca cattctttta cttttattgg aaaagtaaaa
    26281 tcaatatcag ctttttgctc tacatatgca atcttcacca gttttttatt gatatcttca
    26341 ttattaatgg agacctctcc ctgatgaggg ataatattta acattgcttt aagcaatgtg
    26401 gattttccag caccatttgg accaataata cctgttaaag caccttcttg tatttttaaa
    26461 gaaatctcgt tcaatgctaa aaaatcgtta taacaaacat taagcttttt cacttctaac
    26521 ataactttct ccttctaaat gataatgatt atcacgatca tatggtatac aaaaattata
    26581 ttttagtcaa ccctaaattt tatagtttag atttttttct gttgctgaag attagaatga
    26641 caaaactatt tgaaactacg cgtatagtaa gtagctttca aaatatataa aaaaggagcg
    26701 ttctcaaatg agaacatgct cctaaaacta ctttattggc gtatggaaaa attatacttg
    26761 aagtggatag cgggttgaat aagttgataa gcatcaaaac atttataaaa attattcaag
    26821 actatgtctg ttccatatac aaatatgaaa atcagtaaac tccatttaac ttttttcgta
    26881 gtgtgtcttt ttatacatgt ttatcgttat taatagtgaa taggaaaaaa actatttttg
    26941 atcattgaca attaaataca atttttgaat aacgatccct gctattcgag ctagaaactc
    27001 aagtacgcca tgacctgatt agcgacaaat acttagagat ttctttttgt tctgggaaaa
    27061 caaaatgcca tgacaatagc agcaaataat gatacttccg taaggctcgc cgtaaactgg
    27121 ggagaggtga aattcctatg tgcttaccca aagcaattca aaaatttcat aactactatt
    27181 ttaggagatg attttataga atccagagtc tacctgaata aaaaacaaac ctgtgcattt
    27241 ctaggaatcg ataataatac tctagataag tggattcgta tatataattt cccagtaata
    27301 agagttggtg gtgtagttcg ctttgacgta gaagatatta aagactttat gaatacattt
    27361 aaaataactt gaaattttct tctcgccgca tatactaaaa ttagtgaaat atactgcctt
    27421 acttttttta aggagtgagg attttgccta caaagctttc aaatgggaaa tataaaacta
    27481 atctgagata tccaaaaagg tttaaagaaa taacaggaat tgcatcagaa aagtatcaaa
    27541 aaacatttcc aaataggcaa ttggcgatta aagccgaaaa tgatatgaag aagaaaatag
    27601 aaaaagtttt gagagaggag aatgcaaact cattagaatt aaaaggaaaa ataacgttta
    27661 aaaaatttta cgaatctaaa tggcttccta gatatgaatt aggtcaaaca attagatcaa
    27721 acaggcctcc tagtgatata accattagta atacaaaaga tatatttcgg ttgcatattt
    27781 tacctatgtt tggtgaatac gcaatgaatt atctaaatat caatactgag ataatatcag
    27841 atgaattaac caaaaaatcg aaagaatatg caaatataaa aatcataaaa ggatatgttc
    27901 gttccatgtt cgatattgca gaaatactaa attatatcga atttaatcga actactaaaa
    27961 ttattcaaag tatcactgca cctaaaaaaa atgctttaga agaaaaaaga atacaagaag
    28021 gtaagcaagc actatcatca aaagaactta ctaattggat agaagcagta aatgatgatt
    28081 ttaataatca tttacttact tttcatgatt acacactttt tatgattacc ctgtatcttg
    28141 gtgatagaaa aagtgagact tacgctttgc aatggaaata tattgatttt gaaaaacaaa
    28201 cggtccgtct taaacatact ttagataaat atcaaagaaa aaagtttaca aaaggtcgaa
    28261 aagataccgt cattcaagta ccagaagttg taatgacatt gctaagtgaa tggaaatcgg
    28321 tacaagctga tcaattgttg aaattaaaaa ttaagcaaac gttagatcag tatctcttta
    28381 cttatactaa accatctgga gaagtaaatt gtcctgttca tgccgattac ctaaactatc
    28441 gaatcaattc aattaaaaga aggcatcctg acttagttca tttatctcca cacaaactcc
    28501 gtcatactta tgcaactata gctagacaag gtggagcaga tatgaatcaa atttcaaatg
    28561 ctttaacaca ctcagatatt tctacaacaa agatatatgt gaacacacca gatatagttg
    28621 ataaggctgt ttttgaagct tttcagagag gattgaataa gtgtgattga tatgtgttct
    28681 taaatgtgtt cttaaaaaca aaagaagcac tttccaaata attgaaaagt gccagaaacg
    28741 ttgatatata aaggatatta acgttttgag aattgtgaag ctttacgtgc tttcttaaga
    28801 cctggttttt tacgttcgac catacgtgcg tcacgagtaa gtaagccagc acgtttaaga
    28861 gctaaacgga agtcaggatc aacttctagc aatgcacgag cgataccatg acggatagct
    28921 cctgattgtc cagcgtatcc tccgccgttt acgttaacaa atacgtcata tgcgcctttt
    28981 gtttcagtaa cagcgaaagg ttgattgatt acttcacgca agtcagcatg tggaatgtat
    29041 tcttcaacat cttttttatt tacagtaatt ttgccagttc ctggtactag acgtacgcga
    29101 gcaactgcgt ttttacgacg gcctgtgccg atatattgaa cttgtgccaa tgaaattccc
    29161 tcctattaga ttaagtttgt gatgtctaaa acttctggtt gttgtgctgc atgtggatgt
    29221 tctgctccac cgtatacgtt caattttgta aattgtttgc ggcctaaagt gtttttagga
    29281 agcatacctt ttacagaagt ttcgatcaaa cgacgagaat ttttagcacg caattcgcca
    29341 gcagtgattg atttcaatcc tcctgggtag ttgctgtgac ggtaataaat tttgtcagtc
    29401 gcttttttac ctgttaattt cactttgtct gcattgatga cgatgacgaa atcgccagta
    29461 tccaaatgag gtgtgaaagt tggtttattt ttaccacgta gtacagatgc aacaactgtt
    29521 gaaagacgtc ccaatggaac atcagttgcg tctactacat accatttacg ttctacttcg
    29581 cctggtttgg ccatatatgt tgtacgcacg ttattttcct ccaatttaag ttctgatgtt
    29641 tgacatacac ttcgagtttc cggggctctt tgtggggcaa acaataccat ttaacatatt
    29701 acccgctaat ctagtcaatg tcaacgtttt tctgcttgat tgaaaaattt atatgaaact
    29761 ttacttcttt tctttctttt atataaaagg gtcgtttcct gtcaaaatga aaaacaaaaa
    29821 gaggctgtga caaaagtctt gtcacaaccc cttattttga ataaacggtg ttctcctgcc
    29881 aactcctcgg tcacaagtca caatatccat aaaacataag aagctgtttt ttaaaattgt
    29941 ttcttgtgac tcggagctga acggcaggct tacaacctct tggtaaacgg tgttcaatca
    30001 gctgaccctc ggtattagct caaaaaacgg caaaatatga ggaactattt tgacgtttgt
    30061 ttctctaata ctcaggtcaa aacgctgatt tcacaacctc tttttcggtc aagttcaaca
    30121 acagttttta cgcgtcgatc tctggcgctt tgcccaaatg agcttggatc atccagatac
    30181 gtttttctgt ggctgttttg aattcgataa agatatcttg tgttggatcg tcgccttctt
    30241 ctccagaaac ttcgattcct ttttggtaaa gatctgctag ataacgatag cctgcaacaa
    30301 gtgtttccaa acgttctgga atagtacgat cccagcggcc aatttcatca gggatttttg
    30361 tgttatctgc aaattcacgc aatgtagaat aaggtgaacc gtctaatgtg atcaagcgtt
    30421 cggatacttc atctaactga gcgttcaaat catccatgaa ctcatccatc attgggtgca
    30481 atgtaaggaa acctggtcct ctcatgtacc aatgtgtttg gtgaacaaca acagacattt
    30541 ggcttagatc tgcaactaat tgatttaata cttctttggt ttgttggaat ttcatctttc
    30601 ttgcctccta acattttctt acacttaaaa taatagtaga ctttcaatga aaagtacaac
    30661 aaaatcattt acctgagaac gattttcagt tcgctttttt atccgtaaag ccaatcattt
    30721 ttcagaatca gctaattctt ctcgcagttg agcaaattgc tgataagtct gtgctaaatg
    30781 cttttgtgtc gaaacaaata atggatagat tttctgatat tttttatgat cagtttcttg
    30841 tactccaact tgctctttca attctatcaa ttcctgtgct tcactgatac tttggatctt
    30901 tccgatgctt ctccagccta aaatcacagc tcctaatgcg gatgcttctg tgacagtagg
    30961 gaaaacaagc gggtaaccca gtacatcagc catcatctga cgaaaaatcg gagattgagc
    31021 aaacccgcct gtagcacgga tctctgtgag ctgattttcc tggcaaactc cttttaaaat
    31081 ccgataaagg ttgagacaga tcccttctag aatcgccttc atcatttctt tttctgtatg
    31141 ttgccgctgt aaaccgataa aactgccaaa cgcatctgcg tcccaaagcg gtgctcgctc
    31201 accaagtaaa tagggatgaa aaatcagacc attggcaccc gctggcacaa aagcgatagc
    31261 cgaaagcagt gcatcatatg gattttctcc tttttcttta gcagattgaa cgacttcctg
    31321 taaaaatgta tgccctgccc aatcaaagat gccagcacca ttgctagtcg ctcctcccac
    31381 tacccaatgt ttctgatcca gaacataaca aaacgtctcc atttctggat ggaactgagg
    31441 ctgagtagta ataaaacgaa gtgctccgct cgttcctacg gtcaaagcgg cgatcgtatc
    31501 accaaaagca cctaacccta gattagaaag cggcccgtct gcccctccca aaacaaatga
    31561 tatatcattt ggcaaaccta gttctgctgc tgtttttgat ggtagacctg ttacttgata
    31621 ggtggaagga aagacttgcg gcaattgctc tattcgaatg ttcaagtagt caagcacttc
    31681 ttctgcccat tggaattcat ggatatcaaa atatccagtt cctgaagctg ttccgtagtc
    31741 gctgatcagt tcgccaaaca atctataaaa gatatagtct ttgataccga caaaataagc
    31801 agtttttttg aataattcag gttggctgtt ttgcagccac ttgattttgt tcaatggtgt
    31861 cattggatga atcggcgtcc cagtctgccg ataaaattct ttgccaagca cggtatattt
    31921 cagtttttct gcttcgtccg ctgctcggtt atctgcccaa gtgatcattc gagataacgg
    31981 tgttttctct tcatccaaca atatcaggcc atgcatcgca gaggagaagg agacaaattg
    32041 aatgattgtg tctgaaggca actgtttaat tacttctttt ataacggtgc agacaccttg
    32101 aaagatcaat tcctgatctt gttctgccat cccttgtgtt tcttggatca aagggtagcg
    32161 aaaataagcg gatgctgctt cttgggcttc atctgtgaaa gcaactgctt tggtttgtgt
    32221 ggttccgata tctaatccga ttgaaacgtt cattttttca cctcaaattt tatgtatgta
    32281 caaaaattta gtttaagctt gcgtatcttg gttttgaaaa gggggagatg gcgtgttact
    32341 ttattttatc attggttgct gtatcggatc atttttatgt ttaaccgccc agcggattcc
    32401 gttaggtcac tctattattt atcctagatc tcattgtgtc aaatgctgtc gttcgctttc
    32461 ttggtatgaa ttgattccga ttctctcgat catcgtccaa cgatttcgct gccgctattg
    32521 caggtgtcgg ttgcccttct actatttgtt ggcagaagcc ttatgtggtg gtctttttgc
    32581 ctggttcttt actttttctt ccgcaagaaa tttttcgacg ttcatatgga tgctttcggc
    32641 tttgctattt tcgttgatgg atttatttta cttcatggtc cattctcaca cgctattctg
    32701 ttcttgggca attttatgga ttttttggtt gcaggccggc gtcttccaat ggcaaagtgt
    32761 gctgttaacg tttatcgtag gcggtgtgtg ccttcgctat ggtcaatcct atctcggagc
    32821 tggcgatact ctgttgatgc tttcttggag tggtggcttg tctctagaag agttgcttca
    32881 gatcttgttt cttgctagtc tgtttggaat atccgttttt tctgtttact ttatctgtca
    32941 taaaaaaaag ttggaaaagc tgcctttcat tccttttttg agcagtgcgt tgctgattgt
    33001 ccttcatctt aggtattgaa aaaaggttgt gacaaacgac ttgtcacaac ctctgatata
    33061 tagtgttctc ttaccaaatc cttgtgactt ggagctaggc gacaggttca caacctctcc
    33121 actaaatggt gttcaatcag ctgaccctca gtattagctc aaaatgctgg ttttacaacc
    33181 tctgatatac ggtgttccat aagtagcttt ttcggcttct tatttcttga cgccccaatg
    33241 tcgacgaagg aaacctgcac gtccagaacc ttctcgataa agattgtaat ggagcttgtt
    33301 tttcttgtaa taatcctgat ggtaatcttc tgcaggataa aattcgcttc ttggttcgat
    33361 ttttgtcacg attggcttat cgaatcgccc gctgtcttgc aatgcttgtt ttgaacgctc
    33421 tgcaatattt cgttcttctt catcattata gtagatcact ggacgataag aatcaccgcg
    33481 atcagcgaac tgtcctaatg cgtcagtcgg atcggtttgc tgccaataga tttccaccaa
    33541 ctgatcataa gaaataactt ctggatcata ttcgatctct actgcttctg tatgccctgt
    33601 tgtgcctgtt gttacttgtt cataagttgg atttggtaca tgcccgccag tataaccgga
    33661 tgtcacagaa atgatccctg gctgctcttc aaatggtttg accatacacc aaaagcatcc
    33721 gcctgcgaaa actgcttttt ttgtcatata aaatccccct tttttctcat cataccttga
    33781 tttccaaaag gaggataacg ttttgcttgt gacagaaaaa acggaaacta ttatatacgt
    33841 gatacggctt ctgataaatg ccaaaacgaa cgtacttgat caaaaacggt taaatcagga
    33901 atcacatatt cagcagaagg aagatttatg ttttcaccta ttcccaatac atgcatgtta
    33961 ccagccaaag ctgcttgaca accagcttct gaatcttcaa tgaccaagca tgcatttggc
    34021 ggtacattca attgctgtgc tcctttgaga aaaacttctg gatcaggttt tgcttttgat
    34081 acttgcgtgc cgtcaataag gacatcaaaa taacttgtca aattcgtttt ttctaaaatg
    34141 aggcgtgcat ttttgcttgc tgatcccaat ccgatcttga tgtttgtttt tttcaagtac
    34201 tcaagaacag ctattgcacc aggcaaaacg gatgtttcat ccattttttg aatcgcttga
    34261 agatatagat tgtttttttg ctcagcaaag gctgatttct ctttttctga gaatttctgt
    34321 tccatttttc caaatgacag caatcgctct agtgactcgg ttctggatat tcctttcagt
    34381 tgctcgtttt ctttctcagt gaatggaatg gacagctggt tagctaaaac cagccacgct
    34441 tcgtaatgat aacgagctgt gtctacaaga acgccatcta aatcgaatag tgctgctttg
    34501 attttcatcg attacctcct tgaaaatgaa cgacttgaag taaagatgcg atgtttttag
    34561 ctgtgcgatg aagattatca gtggcttgct gtaaagcttc ttgtaaaggc acagctgcag
    34621 gaaggatcgg aaaaacggcc gaaatgccaa catctgctgc tttttcgctt cctttaccaa
    34681 cacttccgca aatagctatc acaggaacgc cctctggtac tcttctagca atcccaactg
    34741 gcgcttttcc ttttagactt tgtccatcta gacgtccttc gcctacgatt acgaggtctg
    34801 cttgctgtac ccgtttatcg aactccagta aatccaatac gtagtcgatg ccagagacta
    34861 ttttccctga agcaaatgct aacagtcctg cacccattcc accaccagca ctactgcccg
    34921 atgcctcgat tacttgaggg gcaaatctca tataaaaatc atgcatggct tggtcagcca
    34981 ctgctaattc attttctttc aagccttttt gtggtccgaa aacaaaagaa gcgccttctt
    35041 ttccgcataa aagattagta acatcagtaa tgacagtgat tttggcttta atcagctcaa
    35101 taggcacgtt cgttttatca actgtagcaa ttttacctag attttctccc agtgcttcta
    35161 ctttttgatt atcttgatcg taaaaagcat aacctagacc ttctgccatg ccgatgccgc
    35221 catcgttggt actacttccg ccaacgccga tgatgatgtg tcgaacacct tgttgcaaca
    35281 attgtcgtat cagttctcct actcctcgcg tggataacct aagtggctgt tgttggttta
    35341 gtgggacatc tttcaatccg acgatttctg ccatttcaaa aatcgcagtt tgaccgtctg
    35401 aagcgtactg gatttctttg tttttgccaa atgcattagt aacgactgct gtttgccgct
    35461 tcaaccttaa actgtctgct aatgatgaaa ccgttccttc tcctccatcg cctataggta
    35521 gcagatcata ttcagcctga acaaaaactt cttgaaaacc tgattgaatg gcttgtgctg
    35581 cctctaaggc tgtcagactt tctttgaaag aatctggcgc aatcagaata cgcattattt
    35641 tcccctccgt tccaagatca gttctttttc cagctggtat tggtctccat tgacaaaaat
    35701 aggtaatgtt tctttcgtct ctttttttat tactatttgt ttttttgtca aaacaaatag
    35761 caacctgtga tttttccaaa taaaaggaaa agaaattttt tcccatgctg aaggaagtct
    35821 tggagaaata gaaagagttc cattcgttac tgtaatacct gaaaaaccaa atataactgc
    35881 tagccatagt gctcctaatg aagcagcatg aattccgtca tctgatgaat gcggatctgg
    35941 tcctaaatca atcaggcacg cttcttggaa taaatgatac gcccattcca tttcttggat
    36001 acggcaagcc tctatggcgt gaatcgcttt actcaacgat gagtcgtgga ttgttttgtt
    36061 ttcatagtaa tgcaaattct tagaaacgat tggttgagaa aaatactcag gtaataagta
    36121 taacagcatg acaagatctg cttgcttgct gacttggagt tcatttactt cgctacgggc
    36181 ataatcaagt aaaatagact ggctgccagg acgttctttg taagtggtca aatcaataac
    36241 tggcttactg agatacgtgt catcttgcgg aatcagctgt tctttgttag gcactggcaa
    36301 atataactta tctaaaaaat tttgacattc tttcaaaaaa agcggatcgc cagcttgata
    36361 tttaacagct aaggccacat tataatgcgc cagatagttc gtatatgcat tattgtcgat
    36421 atattctgtg tattcatctg gaccaatgac tctgtgtatc tctaaatgat catcgacttt
    36481 tacagctctg ctcagccaaa aagctgctgt ttcatgaatg agcgtttttc cctttgtttc
    36541 taaaaacgat tgatcctctg ttgcttggac atactcttga acggcataag caaggtctgc
    36601 actgatatga tgttctgcgg cagctgaagc aacttcttgt cttgttccgg ttttgatatt
    36661 caatgcagca aatttgggtg tctcttcctg accagtgtgt gcgctttccc atgggaaaag
    36721 tgcaccttga tagtgataat ctcttgcttt ttgccgtgcg ccttctaatc gatcataacg
    36781 gtacagcagc aaattctttg ctacttcagg aaactgatat aaaaagaaag gcaggataaa
    36841 aatttccgta tcccagaaaa catgcccttt ataaccttcg ccagttagtc ccttcgcccc
    36901 tacactaaca cttggatcat tgcggggagt cataatctgt aaatggtaag cagcgaagtc
    36961 catcgctaac tgatcgaaag aagaatcgcc ttggatcgtt atccgatgct gatgccaaaa
    37021 atctgtccaa taagacatcg actgctctaa aatttctgta taactaacgc ttttctgggc
    37081 ttgggcaata cttttcttaa acgggtctgt ttgctgaaga gaggtagaga gcacgatgat
    37141 cttctccagc gtaaaggacg tattttttct gatcttttct gaaaaggaag cggtgatccg
    37201 gcgatttttt gcttggaata cacctgcttt attgaattta gctgcaatcc caatatcaat
    37261 tttactttct gttgtctggt aggatgcact gatcaaatcg tcaccaaata ctcgcagttc
    37321 tttttcaacc aactgctgtg taccaaaatt ggtttgctgt ccatcgatac ccgttttcac
    37381 atctacttgg cagtcttcgg tcaacggctt gatgaccact ttcagggctg cctgatggag
    37441 attattttga tcagcaacac gacaagtaat gacttccact ttttttcctt gagcagtttc
    37501 aatgagcgca tagcgtttaa gctctcccat ttctaaatct agtgcccgct cgtatgcaag
    37561 aactttcatc gtctcaagag aaaaatgttc accgtcgatt tttatttccc atctgatgac
    37621 atctgggaga ttcactaact cggctgattc tgctcctaaa ggtcggttat atacaccagc
    37681 tgcaaacatt ccccttactt gattccgata gtcttcctcc tggcttcctc tgataccaaa
    37741 aaatccattt ccaatggtca tctgactcgc atacttattg atatactcta aatcaaactg
    37801 tttttcttct actttcataa ggtaataact tctcctgttt ctgccgaacg atataaattt
    37861 tccaccagat tctggatgac atatccttgc cagccatctg caatcatctg agtctggtca
    37921 ccgagcgccc gattcacaaa atgagccata cttagttcat gtgggtcatt aactggcgtg
    37981 gtcttttgta aggaaaccag ctttccatct acatcttcga atagttcgcc tggatacaga
    38041 ttagcacctg cctgatcgcc acaaaaagcc acattcagtt tgtcttctct gatattcaaa
    38101 gcaaacgaag tattcagttg gatgatcttg ccgttttcta attccagtgt gccaaaaatt
    38161 gcatcttcta ctgtgaattt gcttggatcc catttcccga atttccctga attttttttc
    38221 gtaccaattt tttggaagct gtacgcccga acgcttttta ctttaggaaa tccagtaata
    38281 tacatcgctg tatcaagcat gtgaatgcca taatcgatca aaggcccgcc tccttgaagt
    38341 tctttgtcga taaacgttcc ccagcctgga acaccagacc gacgaagcgc attcgcttct
    38401 acgtaataaa tatccccgag aacatcaaga tgattcttga tcattgttgc ttcgtcagaa
    38461 aaacgatgct ggaatccata agccaaaatc tgcccgtttt tatcggctgt ctctgccatc
    38521 tttcttgctt gatcagctgt catagctggt ggtttctcgc acatcactga agcattagct
    38581 tcgagggcag ccaatacatg ttcgaagtga aagcgattgg gtgtacagac actcacgata
    38641 tcgggagaaa tctcattcag catttgtgta actgatgtat acgcatgaga aaatcctgta
    38701 cgtcggcaaa agtcttctgc tcgctggctg actggatcaa ccacagcggc caattctaag
    38761 acatctgatc ttttttgata ataagcagca tgtacttttt ctgctacttg tccagcgcct
    38821 atgattgcta ctttcttcat ttatttctct ctcctttgct tatttactta ttgttattct
    38881 ttctctattc tttctctaaa tcatttaaat gacgcgctgt ttccagacag tttttcaagt
    38941 attgcaatga ttcctgataa attttcactt cattttctcc tcgcaaacga cattcatata
    39001 ccatataacc ttcatactca ttttgaagta gcgtttgaaa atggcgagta aaatcaattg
    39061 cgccagatcc tggttggtag cgatgattat ctgctaaatg gatatgaccc acatactctt
    39121 tttgcgtagt caatgctttt gaaatatcat cttcttcaat attcatatga taaaaatcag
    39181 cgatgatctg cacatgtttc aattgattag agtcaatgta atgacgcgca tccgctaaca
    39241 aattgatcat atggtcttgg tatcggttca aaggctccaa ataaataacc gtacctgttt
    39301 cttcggctac tttatctaaa taacgaagtg attcgctgac tgctttatag tctccttctt
    39361 tgcttcgggg tgaaaccatc ggcggtaatc gataagtgaa cattccccaa gctgctggga
    39421 taacgattcc tttgcctcca acctcgctta gtgcacgcaa aatctcagca atttgacgta
    39481 caccattaag acgccgttct tcgataaagt caccgatcca cccatcgtag ccgttacaag
    39541 ctgtcactac tgggatccct gtttctttga ttgcctgttt gactgtgtcg atattttcta
    39601 ttaacaactt accgtcgatt tcgtattctt caaatccaca gtctttgata aatctaaatt
    39661 tttctaaaat gtctcttggg aaaaatgctt gatcttgcgt accgaatttc attgttctcc
    39721 tcctcggact tgaacgccca ttttgatact ttcttctggg tgctggtcta cataacgcat
    39781 gtaagcttct gcgctttctg caaatggcac aactggatca atgatttctt cacaatccaa
    39841 gtaaccgttc atcaataatt cccaacatgt gtcctcgatt cgtttacggt tccatcttgg
    39901 gtaatctggg tttggttcac tagatgctcg agcaaagacg atttttgcat tattgaaatg
    39961 tgcttcgcga ccaagattaa acccagctgg aaaaggttta gcaaaggcaa cataagaaat
    40021 gatcccgcca tagccgatcc ctattaaaga agcttgtaat gcggcagcac tgccactcgt
    40081 ttcaataact gaatctactc cacgtttatc tgtcaattcc tttagacgaa aaccgacatc
    40141 tgctttcata ggatctagcg tgtaatcggc accgtgtttt tctgctaatt ctctacgctg
    40201 cgctatggga tcgatcccaa cgatggtagt agcacctgct tttttagcta attgaatcgc
    40261 aatttgcccg atagcgccta aaccgaagat tgctacagca tcacctgctc gaacatgtgc
    40321 ttcccggata ccacctaaag cgaattgagc aggatcgtaa catactgcat ttttccattg
    40381 atccaaccga tcaagtttac gtagtttata attatcaaca ccgtttgtaa tcagcgtttc
    40441 acgaatgggt ccataagtgc agactaactc tcctttttga tactctgtca ccaaactgcc
    40501 cgtttcttcg atcgtaccga caatcatatt tcctaattga aattcgccaa ataccacacc
    40561 gcgttggctt ccttcttctc ttggcaagaa taggcgccat tcttctgaaa attcttcatc
    40621 gataaatgga cttaatcctc taaagtcgac aatttctgtt cctggttttg gagtagcgaa
    40681 aattactcgg atacgtactt cgttcttttc gatctcacgg tcttcgtaat ctttgaatcc
    40741 ggctattctt ggttctaatg caactaattt tttcatcttt tctttcctct ttcttttctt
    40801 tttatccttt tacgccgcct tcgactaaat cacctttaat aaatcgttca gataacgcat
    40861 acataatgat gactggtaaa gcggtcacca atgaagcggc catcattcgt ccccaaatgt
    40921 agtctggtgt actgaataat gtgttcaaac cgattggtaa agtaaatttt tcggaggtcg
    40981 acaggaaaat cgaagcaaat aaataatcat tccacgaaac cataaagcaa taaacgaaga
    41041 cagaaatgat tccagaaata gccaatggta taatgatccg aataatgatc tggatccggc
    41101 taagaccatc gatcattgct gcttcttcaa tatcagtcgg aatcgtgtca aaataacttt
    41161 ttagcatgaa tatcgcagta ggaagtgttt gaacgaccat cgtgataatc agtgctgtat
    41221 tggtatcata caaacctaga ccggaaatga ttttaaataa tggaacaacg atcaaaatac
    41281 cggaaaacat ataaacggtg taaaagctag cattgatttt cgagcgagct ttgaagcgta
    41341 gttttgacaa agcgtatgcc ccgctgatcc caatcactac tgcagttacg gaggctatca
    41401 aggagatcac taaactattt ttgaaataca tgatgaatgg aaaaatctca gcattgaaaa
    41461 tatcctgata atgttcaaaa gtccattctt tcggaaaaat agtttgtgtc gttgcaattg
    41521 cttcttgtga atccttgaat gatgtcatca gcatgatcaa aaatggaaac aaattgacag
    41581 ctaatagcaa caaaatcgtc gcgtaaaata agattttcct gctcttttta tttttcattt
    41641 caagtccacc ttctttcgtg agagcaagac gaccgctgca ataatgatga acaatagaat
    41701 cgatatagct gctgcttttc caagatcatt gaacgcaaag gctgtctgat acaaataaac
    41761 gcctaagata ttgactttat tcgtcagtag atagacatct gtaaacatat agaacatcca
    41821 gatagatcgt aaaacaatca cagttgctag tactggtttg attgccggca gtgtcacgat
    41881 cttgaacttc tgccaagcat ttgcaccgtc catttcagct gcttcataaa gcgttttgtc
    41941 gactgtttgc aaaatagcca aaaatgaaat aaacgcatac ggatagtatc tccagatagc
    42001 aaacaaaacg accataaaga agctagcaac tggctggtcg aaccacagcg gtgcttcgtt
    42061 gaaaagctgc aacctatcta ctaacagatc attgacgatt ccataactat tattgaacat
    42121 atatttccaa gcaaaaatca aagaaataga tggcgtaaca taacttaata aaatcagtga
    42181 acgcaccgtt ttcctaaatc tgaacttgcg gttaagcaaa atagctacgg ctaatcctaa
    42241 agctgtactg cctgctacaa ctaagaatgt gtaagcaact gttaaaccta aagaacgata
    42301 aaattcacta tcacttaaaa tttcaagata attgcttaat ccgataaaaa cggcgtctga
    42361 gttaggattc agcggcatat cgaaaaaact gatctgaata ttggaaagca ttggaatcag
    42421 cactaaaaca gctaataaaa gcatgcttgg tgctaaaagt cccatggcta attttaagtc
    42481 agaatgtttc cttggacgat tcattgaaac ctccccctga taatctgcca atagctatct
    42541 acttttttcg aacaccattg gcagaacttc ttgttatttc gtattttttt gtgcgtcagc
    42601 taaagcttga tcgacatctt cgtttccgac agttacttgg ttcactgctt ttccgatcac
    42661 acctgagctt gtgatatctc ccattttcgt gaaattcttt tccccgacta gtccaaagac
    42721 ttgtacttca tcaaaagcac tagcaatctc agatggcagc tcgccaaaag acttgatgac
    42781 ttcattttct ttgtaaccat ccagttcgac tacttgttta ttcactggtt gagcaccacc
    42841 aggagacatc aagacccact tttccatgtt tttcggttga gataagaaag ctgtgaatgc
    42901 ttcagcagcc tctttttgtt tctcgtctag accagaagaa atcgtcaatc cagatactgt
    42961 gccatagaca gcttgatctt tttgtgttgg gatagcaaaa ccgatatttt ctgagttccc
    43021 cgcttcataa acagaaggca aaatgtaagt tgaatagatt gccattggtg ctgttccatt
    43081 catgaaagca tctttgattt ctgtcacatc attagagcct ggcatcgtgt attgggataa
    43141 gttttgataa aacgacaatg cttctctcat cttttccgta tcgatcgtta cctcgccttc
    43201 atcatccaaa acatttgctc catttgacaa agcaaattga gagaacgctt gctcactcat
    43261 tgttccttca gcagtaggca tagcaattcc atattgttta ttctctttat ctgtgaaatg
    43321 ttgagcaatt tttaaaatat catcccaatt ttctggttct gaaaaaccag cttctgataa
    43381 tttttctttg tcataccaaa taccttgaac ccagccgcta attggtgcag caatatagct
    43441 ttttccatct tctgaacgaa caaggttttt ggctccgtca taataattat cttctccagc
    43501 ttcctcgata acgttttgga ctgccgtctg atcaatcagt tcatctttat ccatcacttt
    43561 agcaaaatcc tgactgactt ccattacttc tggtaattta ccggaacgag ctaacgtaac
    43621 gatttttgta ttgaacgctg attcttctac cggcacttgc ttgattttga tgtctggatt
    43681 ttctttttca aaatcagcaa ccagttcatt gatcacactt aatcgttcct gttcgacaga
    43741 agagtgcatg aattgaatcg ttgtctgttg tgattcgtta gagctttgac tttcaccaca
    43801 agcagtaaga agaaatccag tagctagcag acaggtagta acaataattt ttttcatttt
    43861 tttccacctc ttattttatt attttcagcc acatatattc atacggctgg aatatcaact
    43921 ttcgttcttg actgcaatgg ataatttgtc cagaaaacag gtctaggtaa tctccttttg
    43981 gtaaaagata acctgctgat tgattggaaa aattatggat aactaagact ttctcttcct
    44041 tcgcttcgcg aataaaactg ataaaagaat ctgctgagtc cagtaagtaa atcaaactat
    44101 tcggatggaa ggctttttct ttttttcgaa tagatagcat ctgggtcaat tcaccatata
    44161 ctgctgttct taatggatct gtctgcaact ctttctcaat atctgctaac aaaaattttt
    44221 gccgattgat ttttcgattc tctcctgttt tttctacgcc tttgtaatca tttcgacttc
    44281 ccagcacact ttgaatataa attgccggca cgcctggtaa cgttaccaaa aatgaatgag
    44341 cagctaaaaa gcgtttgctt ctccattccg tcgtatctgt tggagaactt aacgcatcta
    44401 gatacgtagc gtttatctca tatggactgg tcgttccatc tgggtttttc ttgtagttta
    44461 ctcgggcacc ctctttcttc agctgttcca ctaattcaag gatcgttttt tccggcagaa
    44521 tacctcgtac cggattcaat ccgataccgt catgggaggc taaaaaatta aaaaaagttg
    44581 tttttttcga tgcgaaatcg atggttttta accattcttt caacttactt gtattacccg
    44641 aacgaatcgc ttgaagggtc aaaggtggta gcgaaaactg atagaccatc tgtgcttcat
    44701 tctcgccatt cccaaaataa ctgatattat ctttgtgtgg cacatttgtc tcagtgatca
    44761 ataccgttcc tggagctgct tcatcagcga ttgcacggaa taattggatg attttatgcg
    44821 tttctggcag gtgtatacaa gaagttccta gttctttcca taaaaatccc acagcgtcca
    44881 agcgaataaa tgaagcgcct ttttctaagt aaaacaaaag aatatcgatc attttcaata
    44941 aaacttctgg atttttaaaa ttcaagtcta cttgatcttc gctaaaagtc gtccatacgt
    45001 gtttttcttt cccgtttttg tcaatgaacg ttgtaagtaa cgggctggtc cttggtctag
    45061 tcacgctgct tgtatctgta tccagtggca aatcaataaa aaaatctttg tacgtatcat
    45121 tcaagtttaa atattcctga aaccaagtac tttcggcaga catatgattg cagacaaagt
    45181 cgaacattaa ccgtgtttcg cttctcattt cttctatatc ttcccatgtg cccaaacggt
    45241 cgttgattgc ctgataattt acaacggaga aaccatcatc agatgtatat gggaaaaatg
    45301 gcaagaaatg tacaactcta aatgtatctg ccaaatactt ttgataaaat ttcttaaatg
    45361 tacgcaaagt tggttgctgc tcttcaaaaa attgatcccc ataagtaatc aagaaaatat
    45421 cttcttcgtc ccaatgcagt ttgcctgcct ggcctttctt ctgctgataa acactgactt
    45481 tttgttcaat catagaggcg gttttctcag acaaatctga gtcgcgataa atcgcttgta
    45541 aaagttttgt atacatcgga ttcccctttc ttttttggga gcgttcccaa cgatttgatc
    45601 atattatgga aaacgcttac tgtcaatagc atttttttaa ataattttga tatacacaaa
    45661 aatacaaata agaaagctta tgtatcaata attaatagct tcttaaaaaa atatggtctc
    45721 tcatgatcat gcaagctatt cctaaatttt caaaatcata gacaaaaaag aatgtctatt
    45781 gtactatgga attattctaa aacgctccca aaaaggaggt gcatataaaa ttgaaaccta
    45841 ctatttatga cattgcgaaa aaagctaatg tctcaaaatc aactgtctcc agagtattaa
    45901 ataaccagcc aaatatctct gataaagcaa aagcaagtgt acttcaagca atcaaagaat
    45961 tgaattatga accgagcaaa attgcaagag gactgtcagg aaatggtttt gacgcgattc
    46021 tagcattatc tcatcgtact tctcatacga cctatggcaa tccttttttt tcagatgtta
    46081 ttcaatccat ttctgccgtt tctgaacaaa ataacttcga cttgattcta caaacggcaa
    46141 aaacaccaga agaagagcta gaaaaatgcg cgaaaaagat ccaagaaaaa atgatccgag
    46201 gaattattat tttaagctct acgatggatg aaacacttct ttttgaattg gatcaatata
    46261 atattcctat tgtggtaatc ggaaaagtcg gaaacgacta cacgaacatc tactcagtgg
    46321 acacggataa tttccaggat agttacgatt tggtggaaac actgatcaag aatggacacc
    46381 gtcatatcgg ctgtctccat gctccgacag aagtttatgt atccaatgat cgtctcaatg
    46441 gttaccgtca atgcttattt gatcaccacc tagatatccg taatgaatgg attatagacg
    46501 gcggctttcg cttagaagat agcatggcag caatgaatga tttaatgaag ctacgagaaa
    46561 gaccaactgc gattttttct accgatgcat taaaatcatt gagtatttac aaagtggcag
    46621 aacaatctgg aaagcagata cctgatgatc tttcgatcgc aggattcagt gatcccttat
    46681 tgtcctccct attttcgcca cagttgactc ggatagacat tccgacaaat cagttaggaa
    46741 tcaaagccac agaactttta ttccaactaa tccaccacca ataccctgaa gaaaaaagaa
    46801 tcattctttc aaccgagatt actctcacag attcgatcca acaacaagaa atgtagaaca
    46861 gaattataaa agaacagaaa cactgaggat aagtcagtta ctctgttctt ttttcaatca
    46921 ctcatccctc gttaccccct cccggtattt tagcgaattt ttcttatttt ccatctgttt
    46981 tagatagctg tggataacta taatgtttgt gttaaactat tttttccaca gggaagaaaa
    47041 aactattttt aaccacgttg tgtataagtt tttatagttc gtttttttaa acacatttac
    47101 ccttatttca atccatgtta tttggggata cttatctcta aaacataaat agaataacag
    47161 acatgtctac tggttttgtt ttattcgagg aattattcaa taatagctag caaaatatta
    47221 aaaataaccg ctctatcttc ccatcaattt attttgcatt caaaaaatga tctatcttta
    47281 cttttgttga ttgacttctc ttggaagatg atcgttccga taccttaatt ggatacgaac
    47341 cgatgttgtt aagatccttt cccacttttc ttctagttag acaaattatt ttagacaaac
    47401 caaaaaagag gttgtgacaa taatttgtca caacctcttt tttaaaatat ctattttgtt
    47461 tgattgttca ttgcgtcttc tgcagccatt tgcgcttcaa tatctgaaat actgtagaca
    47521 ttttcgcttg ctacaccgac ttcttttggt tccatgtcac gataacgttg cataccagta
    47581 ccagctggga tgatcttacc tataataaca ttttctttca aaccaagaag aggatctttc
    47641 ttaccgcgga tcgcagcatc cgtcaagaca cgagttgttt cttggaatga tgcagcagat
    47701 aagaaactat ttgtttctaa tgatgctttt gtgataccta gtaagacagg tcgagaagtt
    47761 gcaggaacgc ctcctgagac tagcgtattg tagttttgat ctttaaagtc tgcgatgtct
    47821 aataatgtac ctggtaagat atctgtatca cctggatcca ttacacgaac tttacgcagc
    47881 atttgacgaa ccattacttc gatatgcttg tcgccgattt ctaccccttg catacggtaa
    47941 acacgttgta cttcacgaag caagtagttt tcaacagata atacatcgcg aacttgcaac
    48001 aattgttttg gatcgattga tccttctgtc aatggtgcac cacggtggat catatcgcct
    48061 tctgctactt tcatacgtgc tgtataagga acagtatacg tacgtgtatc tgtttttcct
    48121 ttgattgtta cttctttggt acgtgttgca ggatcttctg agatatcgat gacatcccct
    48181 gttacttctg tgatcactgc ttgccctttc ggattacgtg cttcaaagat ttcttggaca
    48241 cgaggaagac cttgagtgat atcgtctccg gcaaccccac ctgtatggaa tgtacgcatc
    48301 gtcaactgag taccaggttc accgattgat tgagcggcga tcgtaccaac tgcttctccg
    48361 acttcaacgt cagagccagt tgcaaggttg cgtccgtaac aatgtttgca gacaccatgt
    48421 tttgtattac atgtgaagac agaacggatt gtcactgttt cgacacctgc atcaacgatt
    48481 tgttttgcga tgtcttcgga aatcaattgg tttcctgcaa tgatcaattc atttgtttca
    48541 ggatgacgta cttctttacg ggtgtaacgt cccactaaac gatcttctaa tgattcgatg
    48601 atttcatttc cctcgcggat ggcttggatt tcaagacctc ggtcagtacc gcaatcttct
    48661 tcacggatga ttacatcttg ggccacgtca accagacgac gagtcaagta acctgaatcg
    48721 gcagtcttca aggctgtatc ggtcatccct ttacgagccc cgtgagttga gataaacatt
    48781 tccaagacgg atagtccttc acggaagttc gaaatgattg gcaattccat gattcgacca
    48841 ttaggagcgg ccatcaaacc acgcattccg gcaagctgtg taaagttgga aatgttacca
    48901 cgagctccag aatcagacat catgaagatt ggatttttcg cttccaaacc ttccatcagt
    48961 ttttgttgga tgctgtcttt tgcagcattc caaacagcaa tgacacgttc ataacgttcg
    49021 tcatctgtga tcagaccacg acggaattgt tttgtaatag tttcaacttg tttatgggca
    49081 ttttcaatga tttcttgttt ttcattcagt acactgatgt cagcgatccc tactgtgata
    49141 ccggcatgtg tcgaatgttt gtaccctaag tctttcatac ggtcaagcat cttagaagtt
    49201 tctgttactt tgaatcgttt gaagacttct gcgatgatgt ttcctaagtt tttcttcttg
    49261 aacggtaata ctaattcttg ctctttgata tgtgcaggaa tatcagtacc agcttcaaca
    49321 aagtatttgt ccggtgtttg gacggtcaag ttgaagtctg ttggttcatt cagataaggg
    49381 aattcaggag gcatgatctc gttgaagatg atcttaccaa ctgttgtgat catcgtacgt
    49441 tctttttgcc attcagtaaa tggtttttca cctaatgagt tagggttcac accgatacgt
    49501 gaatgtaaat gaacatatcc gttgcgccat gcgataacgg cttcgttttg atctctgaag
    49561 accattcctt cgccttcgcg tccttcttct tccattgtca agtagtagtt acctagaacc
    49621 atatcttgag aaggagtaac aactggttta ccatctttag ggttcaagat gttttgagca
    49681 gctaacatca acatacgtgc ttctgcttgt gcttcttcat tcaatggaac gtggacagcc
    49741 atttggtctc catcgaaatc ggcattataa gcttcacata caagtggatg caaacggatt
    49801 gcgcgtcctt gtactaaaac tggttcgaat gcttggatac caagtcggtg aagcgtaggt
    49861 gcccggttca gcaatactgg gtgttccttg attacgtctt ctaaaacatc ccatacttcg
    49921 tcttcttgac gttcgatttt acgtttcgca tttttgatgt ttgaagctag ttcgcgttga
    49981 acaagctcac gcatcacgaa tggtttgaat aattcaatgg ccatttcttt tggaagtcca
    50041 cattggtaca tcttcaagaa aggtccaaca acgataacgg aacgacctga gtagtcgaca
    50101 cgtttaccta gcaagttttg acggaaacga ccttgtttcc ctttcaacat atgagaaaga
    50161 gatttcaatg ggcggttacc tggtccagta actggacgtc cgcgacgacc gttatcgatc
    50221 aatgcatcta ctgcttcttg cagcatacgt ttttcatttt gaacgatgat tccaggtgca
    50281 ttcaagtcta acaaacgttt caaacggtta ttacggttga ttacacgacg gtaaagatca
    50341 ttcaagtcac ttgttgcaaa acgtccacct tctaattgaa ccattgggcg taaatctggt
    50401 ggaataacag ggataacgtc cataaccatc cagcttggtt gatttcctga agcgcggaat
    50461 gcttctaaaa tatccaaacg gcggattgca cgtgtacgtt tttgtccttg tgcagatttc
    50521 aattcttctt tcaattcggc tgcttctgca tctagatcaa cattatcaag taattgttta
    50581 atcgcttctg cacccatagc agcttggaat tcttgtccat attgttcacg tttttcgcga
    50641 tattcgcgtt ctgtcaacaa ttgttttttc tctaaattcg tatttcctgg ttcgatgact
    50701 acatatgaag caaaataaat gatttcttcc aaagcgcgtg ggctcatgtc taatacaaga
    50761 cccatacgtg aaggaatccc tttaaagtac cagatatgtg aaacaggagc tgctaattca
    50821 atatgtccca tgcgttcacg gcgaactttc gaacgagtaa cttccactcc gcatcgatca
    50881 caaacgattc ctttataacg gatacgtttg tattttccgc aggcacattc ccagtctttt
    50941 gtaggaccaa aaatccgctc acagaacaac ccttcacgtt caggttttaa ggtacgatag
    51001 ttaatcgtct caggtttttt tacttcaccg taagaccagc ttctgatttt ttcgggagaa
    51061 gccaaaccta tttgcatact ttcgaattta tttacatcga tcaaaagggg ttccctccat
    51121 ttcatttaat ggcaaaccgc aagacggctg atcttcgacc gccttgcagc tcatagaccg
    51181 gattagtcta aattatcttc tgcggtttca aagttttgga tgacatcgtc tttttcatct
    51241 tccacttgtt cagcagcttt cttttctaat tctttggcag tttgctgttc ggcaaatttt
    51301 gtcaaagcat caacagtgat caaatcgtcg tcttcatcgt ccatgtcgcg aagttcgatc
    51361 tctgtttctt cgatatccaa gacacgcatg tccagaccta gtgattgcaa ttctttcacc
    51421 aatacgcgga atgattccgg tacacctggt tttggaattg gttcaccctt gacgattgct
    51481 tcgtatgttt tcacacgtcc gactacgtca tctgatttgt atgtcaagat ttcttgtaaa
    51541 gtataagccg caccgtaagc ttccagtgcc catacttcca tttcaccaaa acgttgtcca
    51601 ccaaattgag ctttacctcc caatggttgt tgtgtaacca atgagtaagg tccgattgaa
    51661 cgtgcatgca atttgtcatc gaccatgtga gcaagtttga tcatgtacat cacaccgaca
    51721 gacacacgtc catcaaatgg ttcacctgta cgtccgtcgt acagaacagt tttcgcatcg
    51781 ctggccatac cggcttcacg aactgtttcc catacgtctt catcacttgc accatcaaat
    51841 actggcgttg ctacgtggat acctaactga cgtgcagcca tacccaagtg taattccaat
    51901 acttgcccga tgttcatacg tgaaggcacc cctaaaggat tcagcatgat atcgattggt
    51961 gttccatcag gtaggaatgg catatcttct tccggcataa tacgggatac aaccccttta
    52021 tttccgtgac gaccggccat tttatcccct tcatggattt tacgtttttg aacgatataa
    52081 acacgaacta acatgtttac gcctggtgac aattcatctc cagcttcacg agtaaagatt
    52141 ttcacatcgt gaacgatacc gccgccgcca tgtggtacac gtaatgatgt atcgcggact
    52201 tcacgagctt tttcgccaaa gatcgcatgc aatagacgtt cttctgcaga taattctgtt
    52261 accccttttg gcgtgacttt ccctacaagc aaatcgccat cttggacttc tgcaccaata
    52321 cggatgatac ccatttcgtc taaatctttt aatgcgtctt ccccgacatt agggatttcg
    52381 cgtgtgattt cttcaggtcc taattttgta tcgcgggctt ctgattcata ttcttcaata
    52441 tggatagaag tgtatacgtc atcttttacc aaacgacggc tcatgatgat cgcatcttca
    52501 tagttgtacc cttcccaagt catgaaacca actaaaacgt tttggcctag agccatttcg
    52561 ccttgttcca tagatggtcc gtctgccaga gtatcccctt tttcgacttt ctcacccaaa
    52621 tgaacgattg gacgttggtt gtagcttgtt ccggagtttg aacgacggaa ttttgttaca
    52681 tcgtatttat ctaatgcacc gttgtcgcgg cgtacgcgga tttcagaagc atcaacgtat
    52741 tcaacgacac cgtcatgttt acataacaaa gcagctcctg agtcatgcgc tgatttgtat
    52801 tccataccag taccaaccca tggtgcttga ggattgatca acggcactgc ttgacgctgc
    52861 atgttggcac ccatcaaggc acggttggag tcatcgtttt ccaagaaagg aatacatgct
    52921 gtcgcaacgg caactacttg tttaggtgat acgtccatgt agtctacttt atcaatagat
    52981 acttctaagt tttcactttg agcacgagcc ataacaacgt cttgagcaaa tgttccatct
    53041 tcattcaatg ggctgtttgc ttgggctacg atgtaatggt cttcgatatc agctgtcaag
    53101 tagtcgattt ggtcagtaac acgacctgtt tcacggtcta cacgacgata aggtgtttcg
    53161 ataaatccga atttatttac tttcgcataa ctggacaaac tattgatcaa cccgatgttt
    53221 gggccttcag gagtttcgat cggacacata cggccatagt gggagtagtg aacgtcacga
    53281 acttcataac cggcgcggtc acgagtcaat ccgccaggtc ctaaggctga aagacgacgt
    53341 ttgtgtgtca attcgccaag agggtttgtt tgatccatga actgtgacaa ctgagaagaa
    53401 ccaaagaatt ctttgatact tgccacaact ggacggatat tgatcaattg ttgaggtgtc
    53461 aatgtttcag tatcttggat cgacattctt tcacgaacca cacgttccat acgtgctagc
    53521 ccgatacgga attggttttg taaaagttca ccgactgaac ggatacgacg atttcctaag
    53581 tggtcgatat catcgacatt gccgatacct tccatcaagt tcaagaaata gctcattgaa
    53641 gcaatgatgt cggctggacg aaccgttttt acagctgctt ctggatagcc gtttccgatc
    53701 acgtttactt cacgttcagg gtctcttggt gaaaatactt tgatgacttg gacagtcatt
    53761 ggatctgtga ccacaccgtc ttctgatgga tagtaagtca ctgaatttag gccattatca
    53821 atgaaaggag ctagtgtttc catgacttgg tgagtcagaa ctgttccttt ttcaacgatg
    53881 atctcgcctg tttctggatc aactaatgtt tcagctaatg tcagattcaa tagacgtgtt
    53941 ttcaaatcta attttttgtt tactttgtaa cgaccaacgt ttgctaagtc gtaacgtttt
    54001 ggatcgaaga aacgagcatt cagcaagtta cgtgagctgt ctgctgtttt tggttcgcct
    54061 ggacgcaaac gttcgtaaac gtctttcaaa ccttcttctg ttcttgagtc gcttgcgttt
    54121 ttatgcaagt ctttttcgac tgtattgcgt aatgtttcgc tgtcgccgaa gatttcaaag
    54181 atcgtatcat cggaaccaaa tcctaacgca cgaactaata cggttaaagg aatcttacgt
    54241 gtacggtcga tacgtacata agagatgtct tttgcatcag tttccatttc tagccaagct
    54301 ccacggttag ggataacagt agaaccgaat ccttcttttc cgtttttgtc gacttttcca
    54361 tggaaataga cacctggtga acggactaac tgagaaacga ttactcgctc tgccccgttg
    54421 atgatgaatg ttccttgttc tgtcattaat gggaaatcac cgaagaatac ttcttgtgct
    54481 ttgatttcgc cagtctcgcg gtttgtcaaa cgcaatgtta catgtaatgg tgcagagtag
    54541 tttgcatcat gcgcacgagc ttctgctact gtatactttg gttctttcaa ttcataatct
    54601 acaaattcca aagatagatt gccattgaag tcatcgattg gcaaaatgtc ttcaaacatt
    54661 tctctaagtc cctcatctaa gaaccattgg taagagtctg tttgaatttc gatcaaattt
    54721 ggtaattcca acacttcact gattcttgcg aaacttctac gttcgcgatg ttttccgtat
    54781 tttactacgt gtccagccaa gctcttcacc cctctaaaat gtcattcaaa aagactaccc
    54841 gatcaattgt tacaataata tttcaaatat tatttttagg taacaacaga cattttttgc
    54901 ggcttttagg caaaaaaaaa ccaaaaatag aacattctct tgaaacctta cttcgtgagc
    54961 ttctactttt gtttccttat attaaagccg aaacggacaa tatttcgttt ctgccttttc
    55021 ttcatcagat agtttttgca acttcatata ctacacttat tttctataaa agtcaacact
    55081 tcctggcgtt tcttctgctt tttcgcaaca ttttcttaat gttttcaatt ttatgttttc
    55141 aattatctga caattcttaa ttgacagacc tttttatctc ggttatactt tagaaaattc
    55201 attttgtgga ggaacttcta tgcgaaccaa catcaaccaa atcgcaccaa cacaactgaa
    55261 taacttttac tttagctact ttagataggt ttgtattcat catgatggat acaaacgtta
    55321 aacgacagtt tgtatctatg atggctaaag aactttgtat tgcagcaaaa agccacgtag
    55381 acaaaatgct aagtggctga gcagtgaaaa tacaaagtaa aagtatcaag cattctactt
    55441 tttcaattta ttttccgact tttcaatcca tttggacatt ttgtctgaat ggattttttg
    55501 tttcccaaaa ttaaggagga aaaagaaatg aaaaaaaata taatcatcgg attaggcgct
    55561 gccctgctac tagctttagg aggttgcgca caaaaagaca attccgcaga ggcatcagat
    55621 ggacaaaaaa caattggcgt attgcaactt gtacaacata actcgttaga tgctgcctat
    55681 aaaggattca aagaaggatt agcagaagaa gggtataaag aaggcgataa tctaacaatc
    55741 gattatcaaa atgcccaaaa caatcaagac aacctgaaaa gtatgagtga gaaactcgtc
    55801 aaagaagatc ctgatttgct acttggaatc gccactccag cagcacaatc tttattaaat
    55861 gaaacaacca ctatcccgat gactgtcaca gcggtcacag acttggaaca agcccattta
    55921 gtcacatcca atgaaaaacc tggcggcaat gtgactggca cgacagatat ggtgccaatc
    55981 gaaaaacaaa tcggcttact gttaaatatc gtgccagacg ccaaaacaat cggcatcatg
    56041 tataacaacg gtgaagcaaa ctctaagatc caagctgatt tggcagaaaa agaattgaaa
    56101 aaagcgggcg tcaaagtcaa agtactgaca gcaaattcca caaatgatgt ccaacaagtg
    56161 acgaaaagtc tagctaaaga tgtagatgga atctatattc ctactgataa cacgttcgca
    56221 agtgcggcaa gtgttgtcgg tgaagtcgca aaagaaacaa aaacacctgt cgttgctggc
    56281 gcagttgatc aagtcaaaga cggcggtcta gcaacagtag gaatcgacta cgaaagcttg
    56341 ggcaaacaaa cagggatcat ggcagcaaaa atcttagacg gcaagaatcc aagtgaactt
    56401 cctgtcgaaa cagcgaatga tttggaactg tatgtcaata aagacatggc caaagcatta
    56461 ggcatcaatc cagacagcat caaagaagct gaataaaaca caggaggatt ctcatgacaa
    56521 atttgctctc tcttttctta tcatctgcat cgcaaggggt cttatgggcc ctacttgcga
    56581 ttggtgtata cgtcactttc cgtatcttgg acattgccga cttgactgct gaaggaagct
    56641 ttcctttagg tgcagggatc gcggcagtaa gtattacaaa tggctatcat ccattgacag
    56701 cttgtcttct aggcttttta gggggtgcgg cagcagggct tgtttccgga ctgcttcaca
    56761 caaaattgaa aattcccgcc cttcttgcag ggatcatcac catgactggt ttatattctg
    56821 tcacatcccg tgtgatgggt gcgccgaaca tctctttatt gggtcagcca acgatctttc
    56881 attgggtcga gtcattcggc ataacaaaag acaacgctgt actgatcgtc ggtctgcttt
    56941 ttgctttgat cgttgtatgt ttacttgttt tgttcttacg tacagaaaca ggacttgcta
    57001 ttcgtgcaac tggcgataat ctggcaatga gcgaagcaaa cgggatcaac acagataata
    57061 tgaagatcat cggttatatg atttctaatg gatgcattgc tctttctggc tccttacttg
    57121 cgcaaaacaa cggctttgct gatttaaatt ctggtattgg aacgatcgta attggacttg
    57181 cttctattat catcgcagaa gttttgttac gtaaccagcc actatttctc cgtctcttga
    57241 ctgtcgtttt aggtgcggtc atttatcgtt ttattttagc gttagtcttc gaactgaatg
    57301 ttgaaccaag tgattcgaaa cttgcttctg ctttcgtgct agtgatctgt ctttctttcc
    57361 cgcagatcca gcaaaaattc catttacctc aaaaaaagaa aggcggtagt gtcaaatgaa
    57421 tgacgtctta acattggaaa atctccatca tacctttgaa aaaggaacga tcaacgaaaa
    57481 tcatgtcctg aaaggcatta atctttcctt agatcccggc gacttcgtga cgatcatcgg
    57541 tggaaatggg gctggtaaat ctactttgct gaacagcatc gccggtacat tttctgtcga
    57601 tcagggtcaa attttattga atggaaaaaa tatcacgaaa aaatctgttg tcgaacgttc
    57661 gaaatccatc agtcgcgtat tccaagatcc taaactaggg acagccgttc gtttaacggt
    57721 agaagaaaat cttgctcttg ctatgaaacg aggaaaaaaa cgcggcttct tccgtggtgt
    57781 caaaccacaa gaccgaacat ttttcaaaga ccagctggtt cggctaaatt tggggttgga
    57841 aaatcgttta tcctcagaaa tcggtttgct gtctggagga caacgtcaag ctattacgtt
    57901 attgatggcc acattacagc gtccagaact gatcttatta gatgaacaca ctgcagcgct
    57961 tgaccctgct acttccaaaa cggtgatgga attgacagaa aagctgatca ctgagcagaa
    58021 tctcactgct tttatggtca ctcatgacat ggaagacgcg atccgctatg ggagtcgact
    58081 gatcatgctg catcaaggaa ggatcgtcgt agatatccag caagaggaaa aacagcagct
    58141 cacagtgccc caattattag cgttgttcaa acaaaacagc ggcacggcat taaaagacga
    58201 tcaagtactt ttatcttcat aatcagaaag aggatgggac aaaaaatgaa cgcccaccct
    58261 cttctttttt ctctttcctt ctttcgtttt tgctttatgt atcaaccgtt gtttcttcga
    58321 aaactatttt gttctctcct tcttcctgat agaatggatg ggaaaggagc ttgatgccat
    58381 gacaacgaaa gacaaggtac ttcaattatt gaaaaagaca ccagattttc tttccggaga
    58441 aaagttggca cagcaactgg aagtttcccg gacaagtatt tggaaagcca tcaaagaatt
    58501 ggaaaaggca ggctatcgtt ttgagcacgg gcctacaggg tatcgttacc tgccctctga
    58561 cgtactagat gcaaaagaaa tccagcaaga gctacggtcg tttattcctg atttgaaagt
    58621 acaaacgatg ttgacttctg aatctacgat gaaagatgcc aaactggcag cagctcaagg
    58681 agattctgtt tcgaagttat ttgttgctga tacacaagaa caagctcgtg gacgttttgg
    58741 acggcctttt ttcgctgaac ctggacgagg aatctacatg agtctcttgc tccatccaga
    58801 caaacggttt gatgaattgc ctcaatatac gatcatcgca gccagtgcct gtgtgaaagc
    58861 cattgaatct gtaaccggtc gttctgcttc gatcaaatgg gtcaatgata tctacatgga
    58921 tggaaaaaaa gtctgtggga ttttatctga agcagtcagt gatatggaaa gcggcaggat
    58981 cagtcatatc atcattggga tcgggcttaa tttttctgtt cctcaaaatc attttccagc
    59041 tgatctccaa gaaaaagcca tctctgtctt tcccgacgga caagctagtg tcacaaggaa
    59101 tcagctaatc agcaaaatct ggcaagaatt gttcacttat ctttctcacc cagaaaaagc
    59161 gttggacttc tataaagaaa aatcattcgt attgggaaaa accgtgtcgt tcaatcaaaa
    59221 aggaacgact tatgtcggtc ttgcaaaaga aatcggtgat catggcgaac tgatcgtcca
    59281 aacaaatgaa cagaagatga gtttgtcttc tggcgaaatc agtctcacat ctatccgtac
    59341 gcaataatta tcagatttca caaaaggaca cctgatctca tacagtgtcc ttttttctga
    59401 attctcttca aaagactctc cttattctat cagctgacag tttctctatc ctctagaaaa
    59461 aggtattcta aaaatatcta taaagcatgg tacactttct ttaaatgaat caatgcaaaa
    59521 aacaagagag tcatcagttt acacgatcaa aagagatagc aggccgcttc ctttttcaat
    59581 cttatagata gaccatgggt aacgataaaa acaaaaaaga acttgcgact tattttgccg
    59641 taagttcttt tttagctagt gccttaatct cttaatccca acaaacgtct cgcatagatc
    59701 acctgaccag agtgcatagc agcatcatca ataatagaaa cgagacgcat tcctcgtgtg
    59761 actggtggtg tccagttctc gtcaattact tcatctagct cggaaggttc cagccttctt
    59821 aagtaagaaa tagcaaaatc agcagctcct cgcaagtatc ctagcagatc ttctatttga
    59881 ttagcttgga tctgctttgc ttgttccaga ctatgcttcc aatcttgttc gttatctgct
    59941 atcgtcatcg gaaaccggct ttcccagtct tgactatgcc aaattggaga agttcctgct
    60001 aaatcagcaa tctggaaatc taattctcga gcggtatgcc aagccagcca agtcattgat
    60061 ttgatctgcg gggctctatc ttccactgga aaacgattgg cctcctcaat agagacgcct
    60121 tcaaacaaac gttcaaaccg atcgtatgcc cgatccacac tctcgcatag catatctaaa
    60181 taatccattt attttcctcc tttttattta tcctacttac tttttcctaa ttctaggata
    60241 cctacttata tgttactaca cacccttatt ttttacactt taacagtcac catcatcaat
    60301 agtttggaga ttgaaagaaa aagatagtgt cgaactatca attgaacaag aagcaacaat
    60361 atgaacgttt cattaatatt ctttaaataa acaatgattt ttcaaacgaa cagtttaaga
    60421 ctaggagaaa aagagtataa taggaaaatg tttgaaagga aaggaagggg aaaaatgaaa
    60481 aaaagggcaa gtttactagt cctagttgct ttgttgttca atatcttttc gtatggtgtg
    60541 gccgtttcag cggaaacata cattcctaaa gaccctggga atatttataa tgatctaact
    60601 ggtaactatg gcttgttagg aattgctagt caattccacg tatttgctaa aaataagaca
    60661 actgtgaatg cacatacaga cggaaatata gcaacacatg aacttgatgc aaaaaataat
    60721 tttggtacag agcttcatga aggtttattg aaccaagaaa taaattatat acaaaaaact
    60781 gattctttcg ctaattcttc aggcatacct acaggtagca caagaatgaa taaatttgtc
    60841 gttggcagta acgtggatgt agcacttcaa gatggtcatc ctacagtcaa tggatccagg
    60901 cttgatcact taactccaga tgatttttat aaagatcgtg ctgggcatga atatattgat
    60961 ttcgagcagg aatttgctaa attgaatgta gcagcaaatg atcttgcaac gatcactcca
    61021 gctaaaacat atactgctgc agattttcca gacatgaaca atcggacaat cgatttaact
    61081 gatttatcag ataccgggtt tttattggta aatatcgatg cagaagtgtt aacgatgaat
    61141 acgccacttc aaatcattaa tccaaatgat caagtagttg tatttaatgt catcaacagt
    61201 tcttctgcat tgaacgtaca gtcaccaatc aaatacaatg atcgttcaaa tcatgaaaca
    61261 gaagattttt cggatgcgaa tatctcttgg aattttggaa atgagatgac tgatttgaca
    61321 atcagtgctc cttttcaagg aacaatcctt gcgcctaatg ctactatccg cattactcaa
    61381 aaccaagacg gaacgattat aggaacaaat gtgattttag atgcagctac caatcgttgg
    61441 gatcctaacg aaatatttat cagcgacaat ggtacagata cgacagacac aagcgacagt
    61501 acagatacat ctaccacaag tgatagctca gattcatcaa cttcaagcga cagtacagat
    61561 actacctcta ccacaa
//
LOCUS       DACDXO010000008        60193 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E23605_2017
            SAMN12639989-rid9881463.denovo.008, whole genome shotgun sequence.
ACCESSION   DACDXO010000008 DACDXO010000000
VERSION     DACDXO010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639989
            Sequence Read Archive: SRR10023389
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 60193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741914.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:42:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,972
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 33 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 17 of 73
            Pseudo Genes (multiple problems)  :: 10 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60193
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639989-rid9881463.denovo.008"
                     /isolate="E23605_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(114..473)
                     /gene="rplT"
                     /locus_tag="IWM35_000630"
     CDS             complement(114..473)
                     /gene="rplT"
                     /locus_tag="IWM35_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HAP9918505.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            complement(526..726)
                     /gene="rpmI"
                     /locus_tag="IWM35_000631"
     CDS             complement(526..726)
                     /gene="rpmI"
                     /locus_tag="IWM35_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HAP9918506.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            complement(798..1322)
                     /gene="infC"
                     /locus_tag="IWM35_000632"
     CDS             complement(798..1322)
                     /gene="infC"
                     /locus_tag="IWM35_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HAP9918507.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     gene            1642..2274
                     /locus_tag="IWM35_000633"
     CDS             1642..2274
                     /locus_tag="IWM35_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HAP9918508.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGDQSYLDAKN
                     MVLRESHIQKLTMQKTVLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTAIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     gene            complement(2276..3181)
                     /locus_tag="IWM35_000634"
     CDS             complement(2276..3181)
                     /locus_tag="IWM35_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAP9918509.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWGIALIISLVLSIWMLIALWRRIR"
     gene            complement(3266..3919)
                     /locus_tag="IWM35_000635"
     CDS             complement(3266..3919)
                     /locus_tag="IWM35_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAP9918510.1"
                     /translation="MIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFYAN
                     PEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEVRV
                     YDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYDYY
                     KELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVAI"
     gene            4241..5185
                     /gene="mvk"
                     /locus_tag="IWM35_000636"
     CDS             4241..5185
                     /gene="mvk"
                     /locus_tag="IWM35_000636"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HAP9918511.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            5178..6155
                     /gene="mvaD"
                     /locus_tag="IWM35_000637"
     CDS             5178..6155
                     /gene="mvaD"
                     /locus_tag="IWM35_000637"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HAP9918512.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKQGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            6168..7253
                     /locus_tag="IWM35_000638"
     CDS             6168..7253
                     /locus_tag="IWM35_000638"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HAP9918513.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            7250..8314
                     /locus_tag="IWM35_000639"
     CDS             7250..8314
                     /locus_tag="IWM35_000639"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HAP9918514.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKVPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAKEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(8563..9366)
                     /locus_tag="IWM35_000640"
     CDS             complement(8563..9366)
                     /locus_tag="IWM35_000640"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HAP9918515.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIPDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(9386..10228)
                     /gene="kduI"
                     /locus_tag="IWM35_000641"
     CDS             complement(9386..10228)
                     /gene="kduI"
                     /locus_tag="IWM35_000641"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HAP9918516.1"
                     /translation="MQEMETRYTHSPEDIRHYSTEQLRKEFLVEKVFEPGKISLTYTH
                     NDRMIFGGVTPTEKELEITLDKELGVDYFLERRELGVINIGGPGFIEIDGQKEEMKKQ
                     DGYYIGKETRHVVFSSQDAENPAKFYISSVPAHHKYPNVKISIDQVKPMETGEGLTLN
                     ERKIYQYIHPNVCESCQLQMGYTILEPGSAWNTMPCHTHERRMEAYVYFDYANEDTRV
                     FHMMGKPDETKHLVVDNEQAIISPSWSIHSGVGTSNYSFIWAMCGENITYTDMDMVQM
                     DQLK"
     gene            complement(10244..11251)
                     /locus_tag="IWM35_000642"
     CDS             complement(10244..11251)
                     /locus_tag="IWM35_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HAP9918517.1"
                     /translation="MGKVVTLGEIMLRLSTEQGTRIDSSERFAAHYGGGEANVAISLA
                     NYGHEAIFATKVPDNALGKAVKKHLQCYGVDTKQVLSGGSRLGTYYMETGSGERAASV
                     IYDRAGSSFAEMDHLEWSLEELFQGIDIFHVSGITPALSKKWQKMTKQVIEAAKKAGC
                     LISFDINYRGKLWTQQEASEVIHQLLPLVDICSAGKMDALYLLGIEKAPEAEEQPLIY
                     YYQKMQEKFPNIQVFYSTKRTVHSASENELKGTLWMNHEYVESQVHRITPIVDRVGGG
                     DAFAGGVLHGILSNMSPQEIIDFATAASALKHTIHGDCNQFSQEEVAQFLACGSGKII
                     R"
     gene            complement(11258..11902)
                     /locus_tag="IWM35_000643"
     CDS             complement(11258..11902)
                     /locus_tag="IWM35_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HAP9918518.1"
                     /translation="MKKIDILSRLKNAGVIAVVRGKSKEEALNACHAIIKGGLTGIEL
                     TFTVPQADQVIKELLSFYKDQPEIVIGAGTVLDAVTARLAILAGAEYIVSPSFDQETA
                     EMCNLYQIPYLPGCMTITEIKTALKSGVDIVKLFPGSAYGPSIISAFKAPMPQVNIMP
                     TGGVSLDNMKEWFDAGVVTVGVGGNLLAPAATGDFDKVTEVAQQYAAKMKEIKR"
     gene            complement(12002..12760)
                     /locus_tag="IWM35_000644"
     CDS             complement(12002..12760)
                     /locus_tag="IWM35_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HAP9918519.1"
                     /translation="MENKKPYGTVLIKASKILDYLAENQDVSLQTIAKGVGMTSSTVL
                     KILDTLLMIGYVNKNSEKNYRLGAKLIRYANKNIEQIDLVELTLPFLEQLQQKIDETI
                     HLGVLDNNEILYVNKLDPKHQTIRMSSKIGITRPLYNSAMGKAVLAEFSEEQYTDYLE
                     KHPLIPYTEYTITNALRLKKEIKTVKETHIAFDDEEIERDIFCIGASIVKNEQIIGAF
                     SVSMPKYRLTDETKEQIISALKQTKSEIEQRIQK"
     gene            complement(12887..14038)
                     /gene="fucO"
                     /locus_tag="IWM35_000645"
     CDS             complement(12887..14038)
                     /gene="fucO"
                     /locus_tag="IWM35_000645"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HAP9918520.1"
                     /translation="MSNRMILNETSYFGKGAIESIVPEFQQRGFGKAAVITDKGLIEH
                     GIATKVTELLDKAAIAYALYDGIVPNPTIQNVKDGVAFVKEAEADCLIAIGGGSPIDT
                     AKAIGIILTNPEFSDVISLEGVADTKNPCLPILAIPTTSGTAAEVTINYVITDEVNHR
                     KFVCVDPHDIPIVAFIDSDMMMGMPKKLAASTGMDAMTHAIEGFITKGAWEMTDMLHL
                     KAIEIIGHSLEASVDGDQNGREKMALGQYIAGMGFSNVGLGLVHGMAHPLSAWYNIPH
                     GVACAALLPTVMKYNKEYTGEKYREIALVLGIKGAAEMSLEDVREAACGEIDRLSKAV
                     GIPETISELGVKEADIPAIAEDALRDVCTPGNPRETTVEEIIALYQSLM"
     gene            complement(14120..14431)
                     /gene="rhaM"
                     /locus_tag="IWM35_000646"
     CDS             complement(14120..14431)
                     /gene="rhaM"
                     /locus_tag="IWM35_000646"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HAP9918521.1"
                     /translation="MVKKAFCMKVYPEKQEEYKKRHDELWPEMRTALREHGVIDYSIY
                     LDPSTSLLFGYLVIEDEKLWEKMANTSINQKWWKYMEDIMETNPDASPVSWELMPVFE
                     L"
     gene            complement(14433..15278)
                     /gene="rhaD"
                     /locus_tag="IWM35_000647"
     CDS             complement(14433..15278)
                     /gene="rhaD"
                     /locus_tag="IWM35_000647"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HAP9918522.1"
                     /translation="MKKIDILQAPYVKEMIETTSNLYRLGWDERNGGNISYLLKEEEI
                     TPFLDPEKVIRRISMIFDASRLAGCYFIVTGSGRYFKNVASEPAENLGIVRVAEDGKT
                     LELLWGLENECLPTSELPSHFMSHIARLEVDPDNRIVMHCHASHLLAMSFTHELDERS
                     FSRTLWQMCTECLVVFPEGISIIPWMVPGTNEIGEATAEKMKETRLVLWPQHGIYGSG
                     KDMDEVFGLIETAEKAAEVYTYVKAQGPILQTITDENLWHLADAFGVTPKAGYLEEVH
                     TKAGV"
     gene            complement(15292..16548)
                     /gene="rhaA"
                     /locus_tag="IWM35_000648"
     CDS             complement(15292..16548)
                     /gene="rhaA"
                     /locus_tag="IWM35_000648"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HAP9918523.1"
                     /translation="MNTIERNYEQAKEKYATIGVDTDAVLEKIQDIKISMHCWQGDDV
                     KGFLTPDGELTGGIMATGNFPGAARTPEELRQDLEKAYSLIPGKHKLNLHAIYLDTEE
                     SVDLNEIEPKHFEKWVEWAKKEEIGLDFNPTFFSHPMMKDGFTLAHPDKEVREYWIEH
                     GKRSRKIAEYFGKETGQTSINNFWVPDGMKDNPIDRFTPRERLMESLDEIFTEELDER
                     YVQDAVESKLFGLGAEAYTVGSHEFYMGYGLTRDKLVCLDAGHFHPTEVISNKLSSLA
                     LFSKGIMLHVSRPVRWDSDHVVLMDDELQEIAKELVRNELLEKTNIGLDFFDATINRI
                     AAWVIGTRNTQKALLKAMLEPVERLKEMELAFDFTSRMAYTEELKDFPYADVWNYFCY
                     KNQVPVGLDWLEEVQEYQKDVLELRK"
     gene            complement(16545..18005)
                     /gene="rhaB"
                     /locus_tag="IWM35_000649"
     CDS             complement(16545..18005)
                     /gene="rhaB"
                     /locus_tag="IWM35_000649"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HAP9918524.1"
                     /translation="MNPTFVAIDIGASSGRLMKSTRDAKGSLILEEVHRFKNGFRNKD
                     GHDRWKIDYLIKEILIGFEKLKQTGIDRLFAGIDTWAVDYCLIDEQGDRINDPIAYRD
                     KRTNGAVEQFSEIYSLDKLYEQTGIQIQPFNTLFQLFAEEKENLAKAHKLLLIPDYLG
                     YVFTGNMVTEKTNASTMQLLNAGTKEWEIELLEKIGVSKELFPPLTEPGTILGDLQKE
                     QFSKYDLPDVTFITVASHDTASAVLGTPGQGERWGYISSGTWSLLGIETKIANVSRNA
                     FSENYTNEWGAHNTVRFLKNIMGMWLIQEVARMQEYEYSYAELAELAAKEKPLQQVME
                     INDPRFLNPPNMIEEIQQYCFETEQPVPVSSGAIARCVYDNLALCYAQELIKVEHLTG
                     TKLEKLHIVGGGSNNRLLNQLTADYAGIIVEAGPSEATAIGNVAMQMLSTGQFSNIEQ
                     ARNVIKQSFPCETFYPDENKQNTMEWYVNKIKGVIV"
     gene            18158..19027
                     /locus_tag="IWM35_000650"
     CDS             18158..19027
                     /locus_tag="IWM35_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP9918525.1"
                     /translation="MDYWEKANHSWSEDSKRVILTPTQRSRNLFFYIQEIGYFKAPIP
                     YYTERAHLPSYLIKFTLSGKGSLTYNDNQQQLKAGDLFFIDCQEYQRYQTISEEPWEM
                     DWIHLNGQNVQSFYQEFTKDGHNTFHSTGDPSENKIHHLITELIHMQQGTNARTDFQS
                     SVAIHELLNELILQKYQLDFEEEDIPAYVYKLKQYLDEHFKETITLDQLEQQFLLNKY
                     QLSKDFSKFIGSPPIDYLISKKLSYAKDLLRYTEFTVRMISEEIGIENFAYFSRLFKA
                     KTGLSPRDFRKFS"
     gene            complement(19153..20052)
                     /locus_tag="IWM35_000651"
     CDS             complement(19153..20052)
                     /locus_tag="IWM35_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP9918526.1"
                     /translation="MLQKKISGEQVNTMVSDQYEIYHVKDVVSQNKTIYHHHDFYEIH
                     ATLKGEATFYLNGRQFTIKAGSVLLIHSNDLHRIVRQSSDVFERVYIFVTPSFLESRS
                     TPWSNLSACFQPMGEKRSRVLQIEQEVLKQKLSFIDHPPDKKIYGEDIKYEQALVEYL
                     IFLNQVVQKEESAQEQNAVVPNERVEQMLQYISKNLSHPLSLEEMEKQFFVSKYHITR
                     EFKKYTGFTFHQYVMKKKLIYAKHLLREYRSSSAVYSKSGFSSYPHFLKAFKREFGIT
                     PKEFLKRDTQKQLVHYEHFEDEQ"
     gene            complement(20217..21041)
                     /locus_tag="IWM35_000652"
     CDS             complement(20217..21041)
                     /locus_tag="IWM35_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAP9918527.1"
                     /translation="MLNITIRGHDLSEVQTIEDLAEKTKEQGIHTLQLALGLSFPEIP
                     SGANEINSGMGNYVKRVLEKQEVTVGILSCYINMIHPDLTIRQELLTKFEQYLRYASS
                     FGASMVASETGCVLPEIQYTEENFTDEAFAEAVSVIRRLVKAGEKYQMMVGIEPGLNH
                     PVYSLARVEQLIQAVDSDYLGIILDPTNLITSSDYQEQVQLVEEAFERFGEKICAVHL
                     KDFRVEQEKIVPVNLGDGVIEYTKIKEIIMKNRPYLYVVLEETKDDGIRYGRSLLE"
     gene            complement(21052..22347)
                     /locus_tag="IWM35_000653"
     CDS             complement(21052..22347)
                     /locus_tag="IWM35_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HAP9918528.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVGALWALGIMLVAFIVAKKNKYPVVEKGQMAHAGKI
                     IKEAIPSLLLIVIIIGGILTGIFTAVEASAIAVIYSLLIAMFYYKTVKLKDIPNMLFQ
                     SVAMSGTIMFLLATSSMMSFAMAFTGIPQAISSLIMGVTDNKFIILLLVNLVLLLVGM
                     FMDVAPAILIFTPIFLPIVTSVGVDPVHYGLFSIMNLCVGSITPPVGTGLYVGASVGG
                     VKAEAMLKPLLPFYGVILVVLFLITYFPQIVMWLPNMLS"
     gene            complement(22363..22842)
                     /locus_tag="IWM35_000654"
     CDS             complement(22363..22842)
                     /locus_tag="IWM35_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HAP9918529.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKAHLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            complement(22848..23876)
                     /locus_tag="IWM35_000655"
     CDS             complement(22848..23876)
                     /locus_tag="IWM35_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HAP9918530.1"
                     /translation="MKKTSLLFAASSLSLGLLGLSGCGNNSSVATVADADVNEIVTLR
                     FAYASNSQPVIDAMNEFGRLVKEKTDGSVQIQYFPDGQLGGERELIELTQSGAVDFTK
                     VSASALESFSKDYSIFSVPYLFNDEEHFFKVMDNEEIMEPIYQSTKNLGFTGLTYYDS
                     GQRSFYMVDGPIHTPEDLKGKKIRVMQSETAIKMVELLGGSPVPMGSDEVYTSLQSNL
                     INGSENNEFVLYTAGHGGVAKYYSYDEHTRVPDIIIMNDAIKEQLTDEQEKAIEEAAK
                     ESTVFEIEAFAKAIEEEKALAQEEHGVQFNDVDNTPFREAVAPLHDIFKNDPTYKDLY
                     QKIQEQGA"
     gene            complement(23893..24999)
                     /locus_tag="IWM35_000656"
     CDS             complement(23893..24999)
                     /locus_tag="IWM35_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HAP9918531.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
     gene            complement(25228..27825)
                     /gene="adhE"
                     /locus_tag="IWM35_000657"
                     /gene_synonym="adhC"
     CDS             complement(25228..27825)
                     /gene="adhE"
                     /locus_tag="IWM35_000657"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HAP9918532.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
     gene            complement(28130..28510)
                     /gene="yajC"
                     /locus_tag="IWM35_000658"
     CDS             complement(28130..28510)
                     /gene="yajC"
                     /locus_tag="IWM35_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HAP9918533.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
     gene            complement(28607..29752)
                     /gene="tgt"
                     /locus_tag="IWM35_000659"
     CDS             complement(28607..29752)
                     /gene="tgt"
                     /locus_tag="IWM35_000659"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010769586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HAP9918534.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            29970..30668
                     /locus_tag="IWM35_000660"
     CDS             29970..30668
                     /locus_tag="IWM35_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HAP9918535.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            complement(30836..33475)
                     /locus_tag="IWM35_000661"
     CDS             complement(30836..33475)
                     /locus_tag="IWM35_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAP9918536.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(34094..35125)
                     /gene="queA"
                     /locus_tag="IWM35_000662"
     CDS             complement(34094..35125)
                     /gene="queA"
                     /locus_tag="IWM35_000662"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HAP9918537.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            35318..36493
                     /locus_tag="IWM35_000663"
     CDS             35318..36493
                     /locus_tag="IWM35_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
                     /protein_id="HAP9918538.1"
                     /translation="MIEFQNVSKIYKGGKVAVEDVNLSFEKGEFICFIGTSGSGKTTT
                     MRMINRMTDPTQGKILIDGKSIQEIDPVELRRQIGYVIQSIGLMPHMTIRENITLVQK
                     LLKVSQEERNKTAEKMIDLVELPREMLDRYPHELSGGQQQRIGVVRALAANQDIILMD
                     EPFGALDPITRDSLQDLVKDLQERLGKTIVFVTHDMDEAIKLASRIAIMSEGRVIQFD
                     TPENILRHPANDFVEELIGEDRLLQAKPNATRVGEVMLNTAITITPEKSLQEAIKLMR
                     EKRVDTLLVTDNSNVLKGFIDVETLNKKRGKVSSVGDILNKHVFYVKESAYLRDTLQR
                     ILKRGLKYVPVVDEQNKVVGILTRASLVDIVYDVIWGEDPATTDVAESATPEESKEV"
     gene            36497..37138
                     /locus_tag="IWM35_000664"
     CDS             36497..37138
                     /locus_tag="IWM35_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9918539.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            37135..38052
                     /locus_tag="IWM35_000665"
     CDS             37135..38052
                     /locus_tag="IWM35_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP9918540.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYGQTWFPSYSFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVDNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            38049..38720
                     /locus_tag="IWM35_000666"
     CDS             38049..38720
                     /locus_tag="IWM35_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9918541.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(39261..39749)
                     /locus_tag="IWM35_000667"
     CDS             complement(39261..39749)
                     /locus_tag="IWM35_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HAP9918542.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(39950..40216)
                     /locus_tag="IWM35_000668"
     CDS             complement(39950..40216)
                     /locus_tag="IWM35_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HAP9918543.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(40216..40479)
                     /locus_tag="IWM35_000669"
     CDS             complement(40216..40479)
                     /locus_tag="IWM35_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAP9918544.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            complement(40472..40744)
                     /locus_tag="IWM35_000670"
     CDS             complement(40472..40744)
                     /locus_tag="IWM35_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9918545.1"
                     /translation="MYWCCCIDSQALDSTFAKDDTLLPITLRLATASRKGVRSERGQR
                     SSLKRGLFLYVKKRKSLLNLSALLYFFDVLNQVRYNYIRKRRRDNG"
     gene            40955..41140
                     /locus_tag="IWM35_000671"
     CDS             40955..41140
                     /locus_tag="IWM35_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP9918546.1"
                     /translation="MALSYGNKKWSLDEAEIPTYEDTWHLRVEVKEEAENIGGCTLFG
                     KGFGNYDQDIKIKMFYS"
     gene            complement(41282..43105)
                     /locus_tag="IWM35_000672"
     CDS             complement(41282..43105)
                     /locus_tag="IWM35_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HAP9918547.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
     gene            complement(43703..46408)
                     /locus_tag="IWM35_000673"
     CDS             complement(43703..46408)
                     /locus_tag="IWM35_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HAP9918548.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
     gene            complement(46714..47661)
                     /locus_tag="IWM35_000674"
     CDS             complement(46714..47661)
                     /locus_tag="IWM35_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HAP9918549.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            47851..48120
                     /gene="rpsN"
                     /locus_tag="IWM35_000675"
     CDS             47851..48120
                     /gene="rpsN"
                     /locus_tag="IWM35_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HAP9918550.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(48296..48658)
                     /locus_tag="IWM35_000676"
     CDS             complement(48296..48658)
                     /locus_tag="IWM35_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAP9918551.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(48669..49790)
                     /locus_tag="IWM35_000677"
     CDS             complement(48669..49790)
                     /locus_tag="IWM35_000677"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HAP9918552.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(49806..50156)
                     /locus_tag="IWM35_000678"
     CDS             complement(49806..50156)
                     /locus_tag="IWM35_000678"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HAP9918553.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
     gene            complement(50286..51797)
                     /locus_tag="IWM35_000679"
     CDS             complement(50286..51797)
                     /locus_tag="IWM35_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAP9918554.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(52119..53471)
                     /locus_tag="IWM35_000680"
     CDS             complement(52119..53471)
                     /locus_tag="IWM35_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HAP9918555.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYFEKEKNKLIDAIKQNLEATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(53624..54700)
                     /locus_tag="IWM35_000681"
     CDS             complement(53624..54700)
                     /locus_tag="IWM35_000681"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HAP9918556.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            54896..56641
                     /locus_tag="IWM35_000682"
     CDS             54896..56641
                     /locus_tag="IWM35_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HAP9918557.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(56921..57358)
                     /locus_tag="IWM35_000683"
     CDS             complement(56921..57358)
                     /locus_tag="IWM35_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HAP9918558.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(57342..58715)
                     /locus_tag="IWM35_000684"
     CDS             complement(57342..58715)
                     /locus_tag="IWM35_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9918559.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(59037..59525)
                     /locus_tag="IWM35_000685"
     CDS             complement(59037..59525)
                     /locus_tag="IWM35_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9918560.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEVFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(59547..60149)
                     /locus_tag="IWM35_000686"
     CDS             complement(59547..60149)
                     /locus_tag="IWM35_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAP9918561.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
BASE COUNT        17104 a        13016 c        10989 g        19084 t
ORIGIN      
        1 tattttagac ttatacgtta ttgtcctgtt tcatttgatg gaggattgta aataaaaaaa
       61 gaggaaagaa cataactttt cgttatcttc tttcctcttt ataattgttt agcttattta
      121 gccaaagctt cttttgcttg gtcagccaat gcagtaaatg ctgctgcatc gttaacagcc
      181 aagtcagcta acattttacg gttgatgtcg atttcagcaa ctttcaaacc gtgcattaat
      241 tttgagtagc tcaagccatt catacgagct gctgcgttga tacgagcaat ccacaattta
      301 cggaagtcgc gtttcttttg acgacgatcg cgatatgcgt agttgtatga attcattact
      361 tgttcttttg ctgttttaaa taaagtgtgt tttgaaccgt agtaaccttt tgctaatttt
      421 agcactcttt tacgacgttt acgagttact gttccacctt taacacgtgc catgtataat
      481 tcctcctaat aaaaataaat cttcagattc tgttatttgt acttcttatt tcatttttgc
      541 tagttgttgg cgaatacgtt tgaagtcgcc actagaaacc atgcttgctt tacgcaattg
      601 acggcgttgt ttttttgttt tgccgtggaa acggtgactt gtaaacgcgc ggaagcgttt
      661 caatccgcct ttaccagtac gtttgaaacg ttttgctgat ccgcggtgtg ttttttgttt
      721 tggcatgact atatttcctc ctcaaaatct ttcgttatcc gactatcggc tgtccaacag
      781 ccagttagtc attcaaatta cttactgtcg tttttcggtg ccagcgtcaa gaacatgctg
      841 cgtccgtcca ttttcgcttt ttgttccact gttgcaatat ccgcagtttc ttcagctaag
      901 cgattcaaga ctttttgacc aatttcttta tgggtaatgg cacggccttt gaaacggata
      961 gaagctttca ctttatcgcc cttttccaag aacttacgtg cattacgaag ttttgtattg
     1021 aagtcattca agtcgatcgt aggacttaag cgaacttctt ttacattgat cactttttgt
     1081 tttttacgcg cttcgcggtc tttcttttgt tgttcgaaac ggtatttacc gtagtccatg
     1141 attcgcgcaa ctggtggttt cgcaccaggg gcaactaaca caagatccaa gtttgcttgt
     1201 tctgcaatat tcaatgcttc tgcttttgtc ttaactccta attgctcacc atctgaaccg
     1261 atcaatcgta actctcgtgc acgaatgccg tcgttaacca tcatatcctt tgctatggtc
     1321 attcacctcc aaatattatg agagaaagaa aagcttaccg aataaacggc aataaaaaaa
     1381 cgagttctct tacagaaccc gcgcgtactt cacctaatag aaccagtgaa aactatctag
     1441 cccagtgaca ttatcaacta aggcgagaag cgggtgcttc tgcttttatt tctcaaccat
     1501 catagtatag caaaaactaa tctttttgtc aatgtttttc atacagtttg tcttcctttt
     1561 ctgaaaatga ttagattagc agactttcta gcagtgattc ccgcttcttt gctatactaa
     1621 agaaaaacga ggtgaaccca catgaaaaaa atcgaaccta ttccaccttc tcttcctctt
     1681 cttacaaagg gagagtttta tttagaagat gaggagatct tctctggtct tctgatcaac
     1741 gggggtgacc aaagctatct cgatgccaaa aatatggtat taagagagag tcatatccaa
     1801 aaacttacga tgcaaaaaac agttttacgt cattttgaat gcagtaatgt catttttgaa
     1861 aaatgtgatt tttccaatct ggagtggatt ggtggcagtt tccaccaagt cgtgtttcat
     1921 caatgtaaat taacaggtac gaattttgcc gaaagttatc ttcgagactg tactttcact
     1981 gattgtattg cttcgatggc ttcctttagt agtacaaact tgaaagctgt ctccttcgat
     2041 cattgtcaat tagaagacag cgaattttat gaggtcactt ggaaaaacct ttttttgtct
     2101 gacaatcagt tgaccggttc gaactggttt cgaacttctc tgaagggatt agactttacg
     2161 actaatttct tcacagcaat cgctttatct caagattctt tggcgggact aatcgtcaac
     2221 caagagcaag cacttgtgat cgcccaagcc cttggtttgc agatcaaaga ctagatcaac
     2281 gaatccgccg ccacagtgca ataagcatcc aaatagataa gaccaaactg atgatcaagg
     2341 cgattcccca tccgaaaacg gaatgggtaa agggtaactg gagattcatt ccgaagaatc
     2401 ctgtcacaat agttggaacg gtcaagatca gcgaccagac cgtcaaaaat ttcatcgtgt
     2461 cattcaagtt gttattcagc aaattattat aagtgccaga gagctggtct aaaatctgcg
     2521 aagctaagtt cgtcatttca actgcttgtc gtgcttcgat cagcgcatcg tccaactctt
     2581 ctttttcctt ttctgacaac ttgcgataaa tagccaatgc cttgatttgt tctaaaagta
     2641 ctacattttg ttttgttcct gtaactaagt aaaccaaacc gatctctaaa tctgaaagtt
     2701 gcagcagatt cttgttcgtt gttttttctc ttaatttcag attaagccgc tgacgttcac
     2761 tattcacttc ctcgattaaa ggaaaaaagg aatcagaaat caaaaacaat gtacgaaaca
     2821 ataaactgta tacactcatg tctggcattc gttccaaaag cgatcgaatt agtcgaatca
     2881 cgtactcggt attcggcgtg gtaaatgtaa acaaaccgtc ttcttttaaa ataaaagcga
     2941 taggactagt ttcataatga ttatcgactt tttgctggtg cggaacattt gatacaagca
     3001 gtaacgcttc atcaaaagaa tcgtactcta cgcgcgcccg ttcattttta tctagagaat
     3061 aagtcagcat ctcgtcacta atgccatatt cttcttttaa gtttttaaca gctagcacat
     3121 cgttttgctg tacattgatc caaaaattac ggtcttgatt aaaatagtgt ttttcccgca
     3181 tactctatcc tcctgacatt tgtttccagt ataaagaaaa aagtcgctca aataaaggtt
     3241 taccctttat ttgagcaact gtaaatcaaa tggctacttt ggtttggcga atctctgcta
     3301 atttttcttc gatcattttc aagacttctt cttttgcttg aggatcttcc acaaaattat
     3361 agcggtctcc atcgatttgg atttttggac tttctttgta ttcttcatac cattgatcgt
     3421 aacgtctgtt caattctttg taataatcgt atagacttgg atcaaaggac aattgttcat
     3481 acgaacgacc acgtttttcg atacgctcca acatcgtatc aaaagataca cgaatatgaa
     3541 ctaataaatc cggatgtttt ttatgggcag catagggaag ttcttccatc atgttttcca
     3601 gcagttcatc atacacacga acttctgttt ctgttgctct tcctagatct gcatttagat
     3661 ggaaaagcag tgaatcttca taaatcgaac ggtccaatac attattgtca tctttcattg
     3721 cttgtttgat gcttgcaaaa cgtttattca aaaaataaat ttgcagcaaa aaagcatact
     3781 gttcagggtt tgcataaaat aagggcaaga cttcattatc atcaatcgat tcataaaatg
     3841 cctgactatc aaagtgttca gccatcattt ctgttaaact tgattttccg gcaccaattg
     3901 tacctgctaa aacgatcacg ctcatttacc tccagctcac ttcatcacta aatattgtgt
     3961 ttaactccaa cctttttaaa tataccccca atatctagta gtgtcaattg tatttttttc
     4021 tagtcaaaga agaaaaaaag tcaaaaaatt cttaagaaaa aagtgttttt tctttagaaa
     4081 acgtattctt agttttactt ctgagatggt tttagtaaaa taaaggagga atgttttttg
     4141 agacccttag tggacaaaaa aattcgtgaa gcagcgcaac tcgttttggg cgctttattt
     4201 tttgcttaaa aatcactttt acgtgagaag gagttagatt atggcaaact atggccaagg
     4261 agaatcaagc ggaaagatca tattgatggg cgagcacgcg gttgtttatg gagaaccagc
     4321 gattgccttt cctttctatg caacaaaagt caccgcattc cttgaagagc tggatgcaat
     4381 ggacgatcaa ctggtttctt cctactattc aggaaattta gccgaagctc ctcatgcatt
     4441 aaaaaatatc aaaaaattat tcattcactt aaaaaaacag catgacatcc aaaaaaactt
     4501 gcaactgacc attgaaagca cgattcctgc tgaacgtgga atgggatcaa gcgctgcagt
     4561 cgccacagca gtcactcgtg ctttttatga ttacttagca tttcctttgt ctcgtgaaat
     4621 actattagaa aatgtccagc tttcggaaaa aatcgcccac ggtaatccta gtggaatcga
     4681 tgcagccgct actagcagct tgcagccgat ttattttaca aaagggcatc ctttcgacta
     4741 cttttctttg aacatcgatg cttttttgat tgtcgctgat acaggaatca aaggacaaac
     4801 aagagaagcc gtcaaagatg tcgctcacct ctttgaaacc cagcctcatg aaactggaca
     4861 aatgatccaa aaattaggat acttgacgaa gcaagcaaaa caagcaatca ttgaaaattc
     4921 accagaaacg ttggcacaga caatggatga atcacaatca cttctggaaa agctgacaat
     4981 cagcaatgat tttcttaatc tattgatcca aacagcaaaa gataccgggg cattgggtgc
     5041 caaattaact ggcggtggac gcggtggctg tatgattgca ttagcacaaa caaaaacaaa
     5101 agcccaagaa atcagtcaag cacttgaaga tgcgggtgct gctgaaactt ggatacaagg
     5161 attaggagta catacctatg tttaaaggca aagcacgcgc atataccaac attgctctaa
     5221 tcaaatattg gggtaagaaa aatgaagaac tcatccttcc aatgaacaac agcctttcgt
     5281 taacattgga tgcattttat acagagacag aagtcatctt ttctgatagc tatatggtag
     5341 atgaatttta tctagatggc accttgcaag acgaaaaagc aacaaaaaaa gtcagtcagt
     5401 ttcttgacct ttttcgtaaa gaagccggcc tctcactaaa agcttcagta atcagtcaga
     5461 atttcgttcc taccgcagct ggattagcct cctctgccag cgggctagct gctttagcag
     5521 gcgcttgcaa tactgctctt aagcttggat tagatgatct ctctctttca agatttgctc
     5581 gacgcgggtc tggttcagct tgccgaagta ttttcggtgg tttcgtcgaa tgggaaaaag
     5641 gccatgacga cttaagttct tacgctaagc cagtcccttc cgattctttc gaagacgatt
     5701 tagcaatggt tttcgttttg atcaacgacc agaaaaaaga agtgtccagc agaaatggga
     5761 tgcgtcggac agtcgaaaca tccagttttt atcaaggctg gttagattcc gttgaagggg
     5821 atctatatca attgaaacaa gcaatcaaaa caaaagattt ccaacttctc ggagaaacga
     5881 tggaaagaaa cggactaaaa atgcatggaa cgactttagc tgcccagcct ccattcactt
     5941 actggtctcc tgacagctta aaagcgatgg atgcggtgcg acaattgaga aagcaaggca
     6001 ttccatgcta ctttacgatg gatgctggcc ctaatgtcaa agttttagtg gaaaacagcc
     6061 atttatctga ggtacaagaa acatttacca aattatttag caaagaacaa gtaattactg
     6121 cacatgctgg tccaggaatt gcgattattg aataaaggga gatttttatg attgaagtat
     6181 ctgcaccagg caaactctat attgccggag aatatgcagt tgttgaaaca ggccatccag
     6241 cagttatcgc tgcagtcgat caattcgtga cagtcactgt agaatctgca cgaaaagtcg
     6301 gaagtatcca atctgcacaa tatagtggga tgcctgtacg ttggacaaga cgcaacggag
     6361 aattagtttt agatattcgg gaaaatcctt ttcattatat ccttgctgct attcgcttga
     6421 ctgaaaagta tgcacaagaa aaaaacattc ttttatcctt ttatgatctg aaagtaacaa
     6481 gtgagttaga cagttcaaat ggccggaaat atggattggg ttctagcggt gccgtgaccg
     6541 ttgcaacggt caaagccttg aatgtttttt acgcgttgaa tttatctcag ttggagattt
     6601 tcaagattgc agcactagcc aatttagcag ttcaagataa tggttcctgc ggcgacatcg
     6661 ctgctagctg ttatggtggc tggatcgctt tctcaacctt cgaccatcct tggctccaag
     6721 aacaagaaac tcagcattct atcagtgagt tacttgccct ggattggcca ggtctatcca
     6781 ttgagccatt gattgctcct gaagatttac gtttattgat tggttggacg ggtagccctg
     6841 cctctacttc tgatttggtc gatcaagttc accgttcgag agaagataaa atggtggctt
     6901 atcagctttt cttaaaaaat agtacagaat gtgtcaacga aatgatcaaa gggtttaaag
     6961 aaaataatgt aacgttgatc caacagatga ttcgaaaaaa ccgacaatta ctgcatgatt
     7021 tatctgcaat cactggggtc gtcatcgaaa cgcctgcttt gaacaaattg tgtaatttag
     7081 ctgaacagta tgaaggagcc gcaaaatctt ctggtgcagg tgggggcgat tgcggaatcg
     7141 taattgttga ccagaaatct ggcattcttc ctttaatgag tgcatgggaa aaagcagaaa
     7201 tcactccact gccgttacat gtctatagcg atcaaagaaa ggaaaaccga tgaatcgaaa
     7261 agacgaacat gtttcactag ctaaagcctt tcatgataaa caaaaaaacg aatttgactt
     7321 cgtacggatc atccataacc ctttgccgca aatagcagtt tctgatgtcg atctaagtac
     7381 acaggcagtt gggtttacac taagcagccc attttatatc aatgcgatga ctggcggaag
     7441 cgaaaaaaca aaaaaaatca accaagatct tgcaatcgtc gcaagagaag cagatttgat
     7501 gattgccact ggttcagtca gcgctgcctt gaaagtccct tctttagccg atacttatac
     7561 gattatgcga caagaatatc ctcacgggaa aatcattgcc aacatcggtg cagggacttc
     7621 tgtcgaaaga gccaaagaag ctatccgatt gtttcacgca gatgccttac agattcattt
     7681 gaatgcacct caagagttgg ttatgcctga gggagaccgt gattttacca attggaaagt
     7741 acttattcaa gaaactcaaa cagccattga cgtccccctc atcgtaaaag aagtcggttt
     7801 tggcatgaca agagaaacgc tcaatgatct agctgcttta ggagttcata cagttgatat
     7861 cagcggtcga agcggaacaa gcttcaccca gatcgaaaac gcccgtcgct ccaagcggga
     7921 actgagttat ttagctgact gggggcaatc gacagtctct tcattgcttg aagcaaatga
     7981 agcagacact tctatggaaa tcctagcctc cggaggtatc cgcaatgcct atgacatatt
     8041 taaagccctt tgtctcggcg caaatgccgt gggtacttca ggaaccgtac tgacgcatct
     8101 gatgaatcac ggcgtagaag aaactattat actgatgaag caatggcaag aagagcttcg
     8161 tctgctttat actatggtcg gcgcaacgaa taccgcggcc ctacaccaac aatctctgat
     8221 cttttccgga ccagtgaagg attggtgcga agcaagaggg atcgacttag tgaagtatgg
     8281 aagacgcaca gaaaagagcg tgggacaaaa gtaaagaaca cttttgtctc acgctctaaa
     8341 cacgtacaaa cggcggaagt aaaagcaact ccttcggaaa taagctgaaa ttcacaaaaa
     8401 tttgaaaagc aattttcgtg aattccttct tatttcttgg agttaaatgc ttctgtccca
     8461 gcctcttttt tcgaataaat gatgtttaat cagctgtcct tcggtattcg ttcaatccgc
     8521 tgatttcaca acctctccac taaacagtgt actaaagctc ttttaaggtt gttttccgat
     8581 ataggcaagt atgcctccgt ccacatacaa gacatggccg ttaacgaaat cagatgcgct
     8641 agaagccaag aagacagatg gtccagccaa atcgattggc tcgccccagc gtgctgctgg
     8701 cgtacgtcca acgataaatt gatcgaatgg atgacgttca ccgttttctt gtgtttcgcg
     8761 taatggagca gtttgcggtg tagcaatata acctggtcca attccattac attggatatt
     8821 gtattgaccg tattcagaag cgatgttttt cgtcagcatt tttaatccgc cttttgccgc
     8881 agcataagca ctgaccgttt cgcgtcctaa ttcactcatc atggagcaga tattgatgat
     8941 tttaccgccg cctttttcga tcatatccgg aataactgct tttgaaacga tgaatggggc
     9001 atttaaatcc acgtcgatta cttggcggaa gtctgccgca tccatttcga tcatcggtgt
     9061 ccgtttgatg atccctgcat tgttgactag aatatcaact gaacctacat cttctttgat
     9121 ctgttgaacc atcgcttgaa ccgcttcttc atcggtcaca tcgcagacat aaccttttgc
     9181 atcgatgcct gcttctttat aatgcgctat accttcatca actgattctt gattcaagtt
     9241 attaaatacg atttttgctc ctgctgtagc taaagagcgt gcaatctcaa atccaatacc
     9301 atagacagca ccggtgacta atgctacttt tccatctaaa cgaaacatat ccatactaaa
     9361 ttccatcatt ttctctccct tatgcttatt ttaattgatc catttgcacc atatccatgt
     9421 ctgtataggt gatattttct ccgcacattg cccagatgaa tgagtaattg cttgtaccta
     9481 cacctgaatg gatcgaccag cttggcgaaa taatggcttg ttcattgtct acaactaaat
     9541 gttttgtttc atctggtttg cccatcatgt ggaacacacg tgtatcttca ttggcataat
     9601 caaagtagac ataagcttcc atacgacgtt catgtgtatg acaaggcatc gtattccaag
     9661 cgcttcctgg ttctaaaatc gtataaccca tttgtaattg acagctttcg catacatttg
     9721 gatggatgta ttggtagatt ttacgttcat tcagtgtcag gccttcacca gtctccattg
     9781 gttttacttg gtcgatactg atttttacat ttggatattt atgatgagct ggaacagaac
     9841 taatatagaa tttagctggg ttttcagcat cttgagaaga aaatacaaca tgtcgtgttt
     9901 cttttccgat atagtatccg tcttgttttt tcatttcttc tttttgccca tcgatctcga
     9961 tgaatcctgg tccaccgata ttgatcacac ctagttcacg acgctctaag aagtaatcaa
    10021 cacctagttc tttgtctaat gtgatttcta attctttttc agtaggtgtc actccaccaa
    10081 agatcattcg atcgttgtgc gtataagtca aactgatttt ccctggttca aatacctttt
    10141 ctactaaaaa ttcttttctc aattgttccg ttgaataatg acggatatct tctggactgt
    10201 gtgtataacg tgtttccatt tcttgcattt tcttctactc cttctatcga atgatttttc
    10261 cagagccgca tgctaaaaat tgtgcgactt cttcttgact aaattggttg cagtccccat
    10321 ggatcgtatg cttcaaggca gaagctgccg tagcaaaatc aatgatttct tgcggagaca
    10381 tattggataa aatcccatgg agcacgccac ctgcaaaagc atctccgcca cctactcgat
    10441 ccacgattgg ggtgattcta tggacttggg attccacata ttcatggttc atccacaatg
    10501 tccccttcaa ttcattttca cttgctgaat gaacggtacg cttggtcgaa taaaacacct
    10561 gaatattcgg gaacttttcc tgcatttttt gataataata aattagaggt tgttcttctg
    10621 cttctggcgc tttctcgata cctaacaaat aaagagcatc catttttcca gctgagcaga
    10681 tatctactaa aggtaaaagc tgatggatta cttcgctggc ttcttgctgt gtccataatt
    10741 ttccacgata gttgatatcg aagctgatca ggcagccagc tttttttgca gcttctatca
    10801 cctgtttcgt cattttttgc cattttttag acaaagctgg ggtgattcct gaaacatgaa
    10861 atatatctat tccttggaat aactcctcta gcgaccattc caaatgatcc atttcagcaa
    10921 aacttgatcc ggcacgatca tagatcactg aggcggcgcg ttcgccacta ccagtttcca
    10981 tataataagt acctagtctt gatccaccag ataatacctg tttcgtatct actccataac
    11041 attgcaagtg ctttttgact gcttttccca atgcgttatc aggtactttt gtagcaaaaa
    11101 tcgcttcatg tccgtaatta gctaaagaaa ttgctacgtt tgcttcccct ccgccataat
    11161 gggcagcgaa tcgttcactt gaatcgatac gggttccctg ctctgtggac aatcgcaaca
    11221 tgatttctcc gagtgtcact acttttccca taagcagcta cctcttgatt tctttcattt
    11281 ttgctgcata ctgttgcgcg acttccgtta ctttgtcaaa atctcctgta gctgcgggtg
    11341 ctaaaaggtt cccacctact ccgactgtta cgacaccagc atcgaaccac tctttcatat
    11401 tgtccagact cacaccgcca gttggcatga tattgacttg aggcatcggc gccttgaagg
    11461 cagaaatgat acttggtcca taagcgcttc caggaaatag tttgacgata tctaccccgc
    11521 ttttcagcgc tgttttgatc tcggtaatag tcatacatcc tggtaagtaa ggaatttgat
    11581 acagattgca catctctgcc gtttcttgat caaaggaagg gctaacaata tactctgcgc
    11641 cagctaagat agccaagcga gctgtgacag cgtctaatac tgtgcctgcg ccaatcacta
    11701 tttctggttg gtctttgtag aatgacaaaa gttctttgat tacttgatct gcttgaggga
    11761 ctgtaaaagt caactcaata ccagttagac cacctttgat gattgcgtgg caagcattca
    11821 aagcttcttc tttgcttttt ccgcgtacca ccgcaatcac tccggcgttt ttcaatcgag
    11881 ataaaatatc aatttttttc atcttctcca cctatttcct atttagaaat tttattttat
    11941 aacttggaaa tctataccca tattatacgg cagaaaagaa aaattgcaaa cgcttttttc
    12001 attatttttg aattctttgc tcgatttcgc tttttgtttg cttgagggca ctgattattt
    12061 gttcttttgt ctcatctgtt aaacgatatt tcggcatact tacactaaat gcaccaatga
    12121 tttgttcgtt tttcacgatt gaggctccta tacaaaaaat atcacgttcg atttcttcat
    12181 cgtcaaatgc gatgtgggtt tcttttaccg ttttgatttc ctttttcaac cgtaatgcat
    12241 tggtgatcgt atattctgtg taagggatca acggatgctt ttcaagataa tcagtgtact
    12301 gctcttcact gaattctgct aatacggctt tgcccatcgc agaattgtat aatggtcggg
    12361 tgattccgat ttttgaagac atgcggatcg tttgatgttt tggatctagt ttgttcacgt
    12421 acaagatctc attgttgtcc aataccccta agtggatagt ctcatcgatc ttttgctgca
    12481 gctgttctaa aaacggtaaa gtcaactcta ccaaatcgat ttgttcaatg tttttattcg
    12541 cgtagcgaat aagctttgca cctaaacgat agtttttctc tgaattttta tttacgtagc
    12601 cgatcatcaa caaagtatct agtattttca agactgtaga agaggtcatt cccacaccct
    12661 ttgcgatcgt ctgcaaagag acatcttggt tttccgctaa atagtctagt attttagagg
    12721 ctttgatcaa tacagttcca taaggctttt tattttccat ataatattct cctatttctt
    12781 tataagaaaa attatagcgg aaataccatc aatttaaaag agatatccag atataaacta
    12841 ccatttctct ttacaggcaa tttaatgttg aagatccgct tcgtttttac atcaaacttt
    12901 gatataaagc aatgatttct tctactgtcg tttctcttgg atttcctggt gtgcatacat
    12961 ctcgcaaagc atcttctgca attgctggaa tatctgcttc ttttacacct aattcagaaa
    13021 tggtttctgg gattccaacc gctttgctca accgatcgat ctcaccacaa gccgcttctc
    13081 ttacatcttc taaagacatt tcagctgctc ctttaatccc tagaacaaga gcgatctcac
    13141 ggtacttttc tccagtatat tctttgttat atttcatgac agttggcaaa agtgctgcac
    13201 acgctacgcc atgaggaatg ttgtaccatg cacttaacgg gtgagccatc ccgtgtacca
    13261 gtcctaaacc gacatttgag aagcccattc ccgcaatata ctgtcctaat gccatttttt
    13321 ctcgcccatt ttgatcgcca tctacagaag cttccaaaga atggccgatg atttcgattg
    13381 cttttaaatg cagcatatca gtcatttccc aagcgccttt tgtgataaac ccttcaatag
    13441 catgcgtcat agcatccata cctgttgatg cagccaattt tttcggcatt cccatcatca
    13501 tatcgctgtc gatgaacgcg acgattggga tatcatgagg atcaacacaa acaaattttc
    13561 ggtgatttac ttcatctgtg atgacgtagt tgatcgttac ttctgctgct gttccagaag
    13621 tcgttggtat agctaatata ggtaaacatg gatttttcgt atcagcaaca ccttctaaac
    13681 taatgacgtc tgaaaactct ggattcgtca aaataatccc aatggctttt gccgtatcga
    13741 ttggcgaacc tccaccgatt gcgatcaaac agtctgcctc tgcctcttta acaaaagcca
    13801 ctccatcttt tacattttgg atcgttggat taggtacgat cccgtcgtat aaagcataag
    13861 cgattgctgc tttatctaga agttctgtta cttttgttgc tataccgtgc tcgatcaatc
    13921 ctttgtcagt aatgacagct gctttgccga atcctcgctg ctgaaattca gggacgatcg
    13981 actcaatcgc tccttttcca aaatacgatg tttcatttaa aatcattcga ttactcattt
    14041 tctattcctc ctaatttgta ttatttttca caataaaaac gaaagaaccc caagcaaccg
    14101 gttgcccagt tgcttgttgc tacagttcaa agactggcat caattcccaa ctgactggag
    14161 aggcatctgg atttgtctcc atgatatctt ccatgtactt ccaccatttt tgattgatag
    14221 atgtatttgc cattttttcc cataactttt catcttcgat cactaagtaa ccaaataaca
    14281 aacttgtact cggatcaaga tagatcgaat agtcgatgac cccgtgttca cgaagtgctg
    14341 tacgcatttc tggccaaagt tcatcatgtc tttttttgta ttcttcttgt ttttctgggt
    14401 aaactttcat gcaaaaagct tttttcacca tgtcagacac ctgctttcgt atgaacttcc
    14461 tccaaataac ctgcttttgg agtgacacca aacgcatctg ctaaatgcca caggttttcg
    14521 tccgtgattg tttgcaagat tggtccttgc gctttgacgt acgtatagac ttctgctgcc
    14581 ttttctgctg tctcgatcaa accaaagact tcatccatgt cttttcctga cccgtagatt
    14641 ccatgttgtg gccataaaac caatcgagtt tctttcattt tttcagctgt tgcttcccca
    14701 atctcatttg ttcccggaac catccacgga atgatggaaa ttccttctgg aaaaacaact
    14761 aagcattccg tacacatttg ccatagcgta cgactaaagg aacgttcgtc taattcatgc
    14821 gtaaaactca tcgctaatag atgactagca tggcaatgca taacgatccg attatctggg
    14881 tctacttcta aacgggcgat atgactcatg aaatgacttg gcaactcgct tgtaggaagg
    14941 cattcgtttt ccagccccca caaaagttcc agtgttttgc catcttcagc aactcgaacg
    15001 attccaagat tctcggcagg ttcgctcgct acatttttaa aatatcttcc tgaaccagtt
    15061 acaataaaat agcatcctgc taaacggctg gcatcaaaaa tcatcgagat gcggcggatc
    15121 actttttcag gatccaagaa cggtgtgatc tcttcttctt ttagtaaata agaaatattc
    15181 ccgccatttc tttcatccca tcccaagcga taaagattcg acgtcgtttc gatcatttct
    15241 ttcacataag gcgcttgtaa aatatctatt tttttcaata tagccacact cctattttct
    15301 taattccaac acgtcttttt gatattcctg gacttcttcc agccaatcca aaccaactgg
    15361 aacttgattt ttgtaacaga aataattcca tacatcggca tacgggaaat ctttcaattc
    15421 ttccgtataa gccattcttg aagtaaagtc aaaagccagc tccatttctt tcaatcgctc
    15481 aactggttcc agcatagctt tcagcagtgc tttttgtgta tttctcgtac cgatgaccca
    15541 agcagcgata cggttgatcg ttgcatcaaa gaaatccaga ccgatatttg ttttttccaa
    15601 caattcgtta cggactaatt ctttagcgat ttcctgcaat tcatcatcca tcaaaaccac
    15661 atggtctgaa tcccaacgca ctggacggga aacatgcaac atgattcctt tgctgaataa
    15721 agctaatgaa gataatttat tggaaatcac ttctgttgga tgaaaatgtc cagcatctag
    15781 acaaacaagt ttgtcacgag tcaacccgta acccatataa aattcatggg aaccgactgt
    15841 ataagcttca gcgcctaaac caaatagttt gctttctact gcatcttgta cataccgttc
    15901 atctaactct tctgtaaaaa tttcatcgag tgattccatt aaacgttccc gtggggtaaa
    15961 acgatcaatc ggattatctt tcatcccatc aggtacccaa aagttattga tacttgtttg
    16021 cccagtttct ttcccaaagt actccgcgat tttgcggctt cttttcccgt gttcgatcca
    16081 gtattcacga acttctttat ctggatgagc caatgtaaag ccgtctttca tcattggatg
    16141 cgaaaagaaa gtcggattga agtcaagacc gatttcttct ttttttgccc actctaccca
    16201 tttttcaaaa tgtttcggct caatttcatt tagatcgaca gattcttctg tatccaaata
    16261 gatagcatga aggttcaatt tgtgtttgcc cggtatcaac gaataagcct tctccagatc
    16321 ttgtctaagc tcttcaggtg ttcgtgcggc tcctggaaaa ttccctgtcg ccatgattcc
    16381 accggtcaat tcgccatccg gtgtcaagaa acctttgaca tcatctcctt gccagcaatg
    16441 catagaaatt ttgatgtcct gtatcttttc tagtacagca tctgtatcta caccgatagt
    16501 tgcatatttt tcttttgctt gttcgtaatt tctttcaatc gtgttcatac gattactcct
    16561 ttgattttat tcacgtacca ttccatcgtg ttttgcttat tttcatctgg gtaaaaagtt
    16621 tcacatggaa acgactgttt gattacattt cttgcttgtt ctatattcga aaactgacct
    16681 gtactgagca tttgcatggc aacattcccg attgctgtgg cttcactagg tcctgcttct
    16741 acaatgattc cggcataatc tgctgtcaat tgattcagta gacgattatt cgatccgccc
    16801 cctacgatgt gcagcttttc caatttcgtt ccagttaagt gttctacttt gatcagctct
    16861 tgtgcgtaac aaagagcaag attatcatac acacaacgag cgatagctcc cgatgagaca
    16921 gggactggct gctctgtttc aaaacagtat tgctgaatct cttctatcat atttggggga
    16981 ttcaaaaatc gagggtcatt gatttccatc acttgctgca gcggtttttc ttttgcagca
    17041 agctctgcca attccgcata actgtactcg tactcttgca ttcgggcaac ttcctggatc
    17101 aaccacatcc ccatgatgtt tttcaaaaaa cgcaccgtgt tatgggcacc ccattcattc
    17161 gtataatttt cagaaaaagc attgcgagaa acattggcga tctttgtttc aatccctaaa
    17221 agcgaccatg tcccactgct gatataaccc caacgttctc cttgacctgg cgttcctaat
    17281 acagctgacg ccgtatcatg acttgctact gtgataaagg tcacatctgg caaatcatat
    17341 tttgaaaatt gttctttttg cagatctcct agtatcgttc ccggctctgt caatggtgga
    17401 aataattcct tcgagacacc gattttttca agaagttcta tttcccattc ttttgtccct
    17461 gcattcagca gctgcatagt agatgcatta gttttctccg tcaccatgtt accagtaaag
    17521 acatacccta aataatcagg tatcagtaac aatttatgtg cttttgccaa attttctttt
    17581 tcttcagcaa acaattggaa caaggtattg aaaggctgga tctgaatccc tgtctgctca
    17641 tacaacttat ctagagaata gatttcagaa aactgttcta ctgctccatt ggtacgcttg
    17701 tcccgataag caatcggatc atttatccga tccccttgtt catcaatcag acagtaatcg
    17761 actgcccatg tatcgatccc cgcaaaaagg cgatcaatcc ctgtctgctt taatttttca
    17821 aatccaatca aaatctcttt gataagataa tcgatcttcc agcgatcgtg accgtctttg
    17881 ttccgaaagc cgtttttgaa ccggtgtact tcttccaaga tcaaagaacc ttttgcatcc
    17941 ctcgtacttt tcattagtcg tccgcttgat gcaccgatat cgatggcaac aaatgttgga
    18001 ttcatttctc cacctctttt actttattgt tgattagcac tttattaata aagcgttatc
    18061 atttattgac aatgctatca taatacagaa tcttatattt tgcttcacac tattttggta
    18121 tataattgca ttatcttgac aagttgggag tgtttacatg gattactggg aaaaagccaa
    18181 ccactcatgg tcggaagact caaaaagagt gatcttgacg cctacacaaa gaagcaggaa
    18241 tttatttttt tatatccaag aaatcggtta cttcaaagca ccaatccctt actatactga
    18301 acgagcacat ttaccttctt acttgataaa gtttactttg tctggcaaag gttcgcttac
    18361 atataatgat aatcagcaac agctaaaagc tggagactta ttttttattg actgccaaga
    18421 atatcagcgt tatcaaacga tcagcgaaga accatgggaa atggattgga tccatttgaa
    18481 tggtcagaac gtccaatcat tttatcagga gttcacaaaa gacggccata acaccttcca
    18541 ttcaacagga gatccatcag aaaacaagat ccaccatttg atcacagaat tgatccacat
    18601 gcaacaaggc acgaatgccc gtaccgattt ccagtcctct gtagctatcc acgagttgct
    18661 gaatgaactg attttgcaaa agtatcagct agatttcgaa gaagaggata ttcccgccta
    18721 cgtttacaaa ctgaagcaat acttagacga acatttcaaa gaaacgatta cacttgatca
    18781 attagaacaa caattcttat tgaataaata tcagctgagc aaagattttt ccaaatttat
    18841 cggttctcca ccaatcgatt atttgatcag caaaaaactt tcttacgcta aagaccttct
    18901 tcggtataca gaatttacgg ttcgaatgat ttccgaagaa atagggattg agaattttgc
    18961 ttactttagc cgcctgttca aagcaaaaac aggactatcg ccgagagact ttcgtaaatt
    19021 cagctgattc atataatatg tacgtcattt tttcaaaata ttttgaaata tatagataaa
    19081 aataaaataa ccgcagtttc tgcttctatg taaacaacag cagctactgc ggttattttt
    19141 tagtgatcat tctcattgtt catcttcaaa atgttcataa tgaaccaact gtttctgtgt
    19201 atcacgtttc aaaaattctt taggcgttat gccgaactct ctcttgaatg ctttcagaaa
    19261 atgcggataa gaagaaaaac cacttttact atatacagca ctagagctgc ggtattctcg
    19321 caacaagtgc ttggcataga tcagcttttt cttcattaca tactgatgaa acgtaaatcc
    19381 agtgtacttt ttaaattctc gtgtgatatg gtatttgctg acaaaaaact gcttttccat
    19441 ttcctctaaa gaaagcggat gagataaatt ttttgagata tattgtaaca tttgttccac
    19501 acgctcatta ggaactacag cattctgttc ttgcgcactc tcttcttttt gtacaacttg
    19561 attgagaaaa atcaagtatt ctaccagagc ttgttcatat tttatgtctt caccgtaaat
    19621 ttttttatca ggaggatggt cgataaatga taatttttgc ttcaatactt cctgttctat
    19681 ttgcaataca cgactcctct tctctcccat tggttgaaaa caagccgata gattagacca
    19741 tggcgtggaa cgactttcta gaaaagaagg ggtgacaaag atatagacac gctcgaatac
    19801 atctgaagat tggcggacga tacgatgcaa atcattggaa tggatgagca gcacacttcc
    19861 tgctttgatc gtaaattgtc gtccgttcaa ataaaaagtc gcttctccct ttaatgtagc
    19921 atggatctca tagaaatcat gatgatgata gatcgtttta ttttggctga cgacatcttt
    19981 gacatgatag atttcatact gatcagaaac catcgtattg acttgttcac cgctgatttt
    20041 cttttggagc atgtgccgca cctccttttg ttcacttatg cacaatttta ttataccacg
    20101 ttttttgtaa tcaaaaagag ctgcttttat atactcttat aaaaaagaag agacgtggca
    20161 tcttctagcc ccgccccctc cattctaaat attagttaac caacgtcttc tttacattat
    20221 tcaagaagac ttcttccata acgaatccca tcatcttttg tttcttcaag aaccacatac
    20281 aggtacggac gattcttcat gatgatttct ttgatttttg tgtactcgat cacaccgtct
    20341 cccaaattta ccggcacgat tttttcctgt tcgacacgaa aatctttcaa atgaaccgca
    20401 catatctttt caccaaaacg ttcaaaagct tcttctacca attggacttg ttcttgataa
    20461 tcactacttg tgatcaaatt ggtcggatct aaaatgattc ctagatagtc cgaatctact
    20521 gcttggatca gctgctctac ccttgctaaa gaatacaccg gatgattcaa acctggttct
    20581 atccccacca tcatttggta tttttctcca gcttttacca agcgacggat aacggacaca
    20641 gcttcagcaa atgcttcatc tgtgaaattt tcttccgtat attggatttc cggcaaaaca
    20701 catccagttt ccgaagcaac catcgacgca ccaaaggaag aagcatagcg aagatactgc
    20761 tcgaactttg tcagcaactc ttgtcgtatc gttaaatctg gatgaatcat attgatatag
    20821 caacttaaaa ttcctacggt cacttcttgc ttttctaaaa ctcgtttgac ataatttccc
    20881 atcccagaat tgatctcatt tgcgccagaa ggtatttcag gaaacgaaag acctaaggcg
    20941 agctgcagtg tatgaatccc ttgttctttt gttttttctg caagatcttc tatcgtttgg
    21001 acttcggaaa gatcatgtcc tctaattgta atgttcaaca taactattct cctaactcag
    21061 catattgggt agccacatga cgatttgcgg gaagtaagta atcaaaaaca agacgactaa
    21121 gataacccca taaaaaggaa gaagcggttt cagcattgcc tctgctttta cgcctcctac
    21181 acttgcaccg acatatagtc ctgtacctac tggcggcgtg atggagccta cacacagatt
    21241 catgattgaa aacaacccgt aatgtacagg atcaacccct acacttgtga cgattggtaa
    21301 aaatattggt gtaaaaatca agatagcagg cgccacatcc ataaacatac ccaccagtaa
    21361 taacacaaga ttgactaata acagaataat aaatttatta tccgttacgc ccataatcaa
    21421 cgaactaatt gcctgaggaa taccggtaaa cgccatggcg aaagacatca tggaagaagt
    21481 agccaataag aacatgatcg tccctgacat tgctactgat tgaaacaaca tattaggtat
    21541 atcttttagt tttaccgttt tatagtaaaa catagcaatc aacagggagt aaatgacagc
    21601 tatcgctgaa gcttctactg ctgtaaatat ccccgtcaag ataccaccga taatgatgac
    21661 aatcaataga aggctaggaa tcgcctcttt tattattttt ccagcatgag ccatctgtcc
    21721 tttttcgaca acagggtatt tattcttttt agcaacgatg aatgctacta gcatgattcc
    21781 tagcgcccac aaagcgccta cgagataacc tcccataaac aaagcagaga tcgaagcacc
    21841 accagcaaca agtgaataca tgataaaagc agtacttgga ggaatgacca tacctgtcgg
    21901 agctgacgcg atattgaccg ctgtcgcaaa cttccggtca tatccttctt ccacttgctg
    21961 agggatcaac actccgccaa tcgcggtaga agcggcaatg gccgagctag agatggaacc
    22021 aaataaagca ttccctaaca cattcgtatg agccaaagcc cccggcacgc gcccgacgaa
    22081 caacatcgca aaatccacta gtttcttagc tatccctcca ttattcatga taatgcccga
    22141 gagaacgaaa aacggaatag cgatcagcga aaaactattg atacttccca ccattttttg
    22201 agctgaacta aaagcagaaa catcaaacgg tataactaac aataaagtca tcatcgatga
    22261 gaatgcaata cttatagcaa tcggcatccc taatacaagc aacacaacaa atacaaaaaa
    22321 taacactaat ccggcttcta ttgccatttt attcctccat ttttatattt tttcttgaat
    22381 tgctggcttg ttgcttaatg tatcgattag attgatcagg ctatagataa tgatgagtac
    22441 ccccgataca ggtactgaca gataaacata tcccatagga atacttaaag aaggcgagat
    22501 ctgccccatt gtcaatgtga cagcattcca tccgccatac accatgatca tcgcagcaaa
    22561 taccagaaat aaactttgaa caacaacttc taaaatttta ctttttttgc cagttaattt
    22621 ttcacttaaa accgttacgg ctaaatgtgc tttttgtccc acaacatacg cggaagacaa
    22681 catggcaaac cagacaagac cgaatcgaac aagctcttcc gtgaccgtat ttggatttcc
    22741 taaaaccgtt ctggaaaata cttgccataa aacaacagcg accatcccaa caagcaaaac
    22801 agctcccagg atctctaata ccttgtttaa aatcattttc atcacagcta cgctccttgc
    22861 tcttggatct tttgataaag gtctttgtag gtaggatcgt ttttgaatat atcatgaagt
    22921 ggggcaactg cctctcgaaa cggtgtatta tctacatcat tgaattggac tccatgttct
    22981 tcttgtgcta aagctttttc ttcttcaatt gcttttgcaa aagcttcaat ttcaaaaacc
    23041 gttgattctt tcgctgcttc ttcaatggct ttttcctgct catcagttag ctgttctttg
    23101 atggcatcat tcatgatgat gatatctggc acacgtgtat gctcatcata ggaatagtat
    23161 ttagccacac caccatgtcc agctgtatac aagacaaatt cattgttttc tgatccgttg
    23221 atcaaattgg attgaagaga cgtgtacact tcatcacttc ccataggtac gggcgaaccg
    23281 cctaaaagct ctaccatttt gatagcggtt tcgctttgca tcacacggat cttctttccc
    23341 ttcaaatctt ccggcgtatg gatcggaccg tctaccatat aaaagctgcg ctgaccagaa
    23401 tcataatagg tcaatccggt aaatcctaaa tttttggtcg attggtagat tggctccatt
    23461 atttcttcat tatccatcac cttaaagaaa tgttcttcat cattgaatag ataaggaact
    23521 gaaaaaatcg aataatcttt agagaaactt tccaacgctg aggcacttac ttttgtgaaa
    23581 tctactgctc ctgactgtgt caattcgatc agttcacgtt ctccacccag ttgtccatct
    23641 gggaaatact ggatctgtac ggaaccatct gttttttctt ttactaaacg cccaaattca
    23701 ttcattgcat caattactgg ctggctgttg cttgcataag caaagcgcaa ggtaactatc
    23761 tcatttacat ctgcatctgc gacagttgcc acactagaat tatttccgca gccgcttaga
    23821 cctagcagac ctaggctaag cgaacttgct gcaaacagca gtgatgtttt tttcatatgg
    23881 gtttcctttc ttttaacaat aagagacgag caattctgtc aaacaaagca ccgttaacga
    23941 ctgtccataa ggcattgctg tgattccgat attccgataa aaatctaaac tgtcccccat
    24001 tcctgtcccg attgaaacgt tttgaacttc tcctttttca tcgatttgtt caagcaatcc
    24061 ttggatagca cgataagcga cttctttata ttcttgtggt aaataacgtt tatggaccgc
    24121 tttcaaaata ccgtaagcaa atcctgcagt tgcagaagat tctacgtaag aagaaggatc
    24181 atccaacaac gtatgccata aaccgctttc agactgataa gtttttaatg cacgcacttg
    24241 tgcttcaagt gtttcgatca ataatttgcg caatgcatct tctttcgata attctaagat
    24301 ttcaatgatc tctggaatag caatcgtcaa ccagcagttt ccacgagccc aaaatgcttc
    24361 ggcatagtta tgatttcctt caaacgtcca gccgtgatac cacaatcctg ttcgtttatc
    24421 catcaagtat ttgatatgga tcataaactg gtaacgagct tcttctaaat attctgggcg
    24481 attcaacagt ttcccgattt ttgccaaagg aagcactgtc atcatcaatg tatcgtccca
    24541 caactgattc ttattttccg gtccatatgt ggcatgctgt aacccgtctt catttgtccg
    24601 aggcatgtca ttcatgaccc attctgccca ttgttctaaa taaggcaaat agcgtgaatc
    24661 tttcgtgtct tcatacaagt aagccatagt caatagcggt gccattgtat tgacgttttt
    24721 gggcggagcg ccttcttcca tacgatcctc aaaccattca ttgaccactt gataagcgcg
    24781 aggatttttt gtcatctggt aatttttata aataccatat agaccgacac cttgtggcca
    24841 attccacact gtccagcttt tgtcatctac tttcagtcca tcaaagtcca acaaaaattc
    24901 accattatcg tctttgatcg ttgtcaggtt ttcaacgagc aggtcaattt tttctttcac
    24961 taattcttta ggtacaaaaa tctcttcttt aataatcatc tttacgccac ctttttaaac
    25021 gctttcattt tatatcctta atatagcagt aataccgtcc actataaata tcgctttttg
    25081 aacaaaaact gtctaaaatt gacctaaatg ttaaataaca aaagtaactt tcagttactt
    25141 ggaaattaaa aaaagaactt ggagaaacaa cgttttccaa gttctttttc atctattttc
    25201 ttatcttttt tctacgcttt cttttgctta tccgttgtaa gcatcaatga tgatctgttt
    25261 caattcagag atcaatggtt ctttcggatt agctgtcgta cattggtctt cgtaagctaa
    25321 ttcagccata cggtctacgg ttgtatctag tgtttcttgt gtgactcctt gagcttttag
    25381 actcatgttg atgccgacag attttcctaa ttcataaaca gccgttgcta aagcttcgac
    25441 taattcctct gttgtttcgc ctttcagacc taagaatttt gcgatatctg cgtaatctgt
    25501 atctgcacgg aaatagtcat atttagggaa catcgcatgt ttttgtggat cttttgcatt
    25561 atagcggata atatgcggta aaagaatcgc atttgtccgt ccatgaggga tcccgtattc
    25621 cccgccgatt ttatgtgcga ctgagtgaca aatacctagg aatgcgttag caaacgccat
    25681 accagccatt gtagaagcgt tgtgcatttt ttcacgtgat tccatatcag gtgtttttac
    25741 tgatttttct aaatagtcaa agactaactt gattgcttgt aggctcaatc cacgtgtata
    25801 atctgaagcc atgactgaaa cataggattc gattgcatgt gttaacacat ccatcccggt
    25861 atctgctgtg acagatgctg gaactgacat gacgaattga ggatcgacga ttgcgacatc
    25921 tggcgtcaat gcataatctg ctaacggata tttcacatgt gtttcactgt cagtgatgac
    25981 cgcaaatggc gttacttcag aaccagtccc tgatgttgtc gggatacata cgaattgtgt
    26041 tttttctggt ttgtcgatct tatatgtccg tttacggata tccaagaatt tttgttttgc
    26101 accaaagaaa gatgtgtctg ggtgttcata gaacatccac attcccttag ctgcgtccat
    26161 tgcagaaccg cctccaagag cgattactgt atctggcttg aagtctgcca tcagtttcgt
    26221 tcctgcgtat acggtatttg tcgatgggtt cggttcaaca tctgagaaga cttcgatctt
    26281 cacatcattt ttacgtcgtg ccaatacttc acgaactgta tcgcagtaac cgaattggac
    26341 cataccaggg tcacaaacga tcatgacacg ttcaacgttt tccattttct ctaagtacaa
    26401 tagagagttc ttttcaaaga agatttttgg cggtaatttg aaccattgca tgttatttct
    26461 ccgtttcgct acagttttga cattgattaa gtttacagca gagacgttac gagaaacaga
    26521 gttcttacca taagaaccgc atcccaaagt caatgacgga atcatttcgt tatagatatc
    26581 accgatgccg ccttctgcag atggtgtatt cactaaaata cgacaagctt tcattcgaag
    26641 accgaatttg acatgcaact cttcgttttc tgaatggata actgctgtat gtcctaatcc
    26701 tccaagattc aacatacctt cacacaaatc aaacgcatgg tccgtgttgt tagctttgat
    26761 catggcaaga actggagaaa gtttttctct tgatagcgga taatctgggc ctacgccttc
    26821 caattcagca atcagcattt tcgttccttt tggtacactg attcctgcca attttgcgat
    26881 ttccatcgca gaatgtccaa cgattgatgg attgaccgca tattttcctt cgttcatcac
    26941 tgcatcttct aatttttgca attcatctgg ttttacgata taaacttggt gtgcttggaa
    27001 ttctgctttt actgctgcat agatttcttt atccacgatc acagcttgtt cagaagcaca
    27061 aatcattcca ttatcaaatg ttttcgaaac gatcaaatcg ttcactgcac gtttaatttt
    27121 tgctgttttt tcaatataag ctggtacgtt acctggacct acacctaaag ctggttttcc
    27181 tgtagagtaa gcagacttga ccattccagc tccacctgtc gctaagacga tcgcgatacc
    27241 tggatggttc attagcatag aagtcgcttc gatcgaagga tgttcgatcc attggataca
    27301 attttcagga gctccggcag cgatagcagc atcacgtacc actcgtgctg cttctgcaga
    27361 agatttttgg gcacttggat ggaaagcaaa aacgattgga tttcttgttt tcaacgcgat
    27421 cattgatttg aaaatcgttg tggaagtcgg attcgttgtt ggagttactc cgcaaaccac
    27481 accgactggt tcagcaattt caatcaaccc tttttggaca tcttcattga tcacaccaac
    27541 tgttttatca tgtttgatat tatgccaaat atattcggac gcatacatat ttttgattgc
    27601 tttatcttcg taaacaccgc ggcctgtttc ctctacagcc atcttcgcta atggcatatg
    27661 ttgatccaaa gctgccattg ccatttcatg cacgatatgg tctacttttt cttgatcaaa
    27721 gctttccatt tctttcaatg ctgcatttgc tttttctgcc aaatcatcaa tcattgcttg
    27781 tacgtcaatt gtcttcgttt tttctttttc tattacttta gccatcatta tcctcctaat
    27841 gggtcatggt tatttgttaa ataattcaca tgtatatcat acaatattta tttttctatg
    27901 taaacacttt tttgtgattt ttttcacagt tatttttaaa tataaaattg ctttatttac
    27961 aggtgtttgt atagaaaaat atataaagtt atttattatt atttatatga cacacccgat
    28021 tattcaatat tgagcaatat atcccatgct cttatctctt tcttttttga ataaaaaatg
    28081 aggtaggaca tctttctgcc ctacctcgtc tttaaataag ctttgatact tatttttctt
    28141 ccttctcttc tgtctcttcc agcgtttctg ttgtttcagt ttttacttca actgtctcag
    28201 ctggttcttc tgttgtttca acagtagtcg ttgcatcatt tgttacgaca gtccctggtt
    28261 tgaccgtttt gattgctgca cgatcgtact caaggaagat cccttcgcag tctaaaacaa
    28321 ctgtccgttt gtcattgtcg atttctgaaa caactccatg taatccgccg atcgttacga
    28381 ctgaatcacc tggtttcatg ctatctaaca agttttgacg ttcttgctgt tgtttctttt
    28441 gcgaacgtga cataaaaaac cacattgcta atagcgcgat aaacatcaca agcattggta
    28501 atgttcccat ttttttcacc tcactaattc tgtatctaca aaaaacactt tagcagaaaa
    28561 atgctgataa cactagtttt ttacttaatt tttaaaagat tttcctttag aagctttttg
    28621 cgttttcttt attgaacccg tattcttcaa aaaaggcttc tctaaattca agcaaattat
    28681 catccatgat tgcttgacgt acttgtttca ttagattcaa caagaagtat aaattgtggt
    28741 aagaagtcaa acgaatccca aatgtttcat cacatttgat caaatggcgg atataggcac
    28801 gtgtatagtt tttacatgta taacaatcac atttctcatc aattggacgg aaatcatggg
    28861 catatttcgc atttttcact actagacggc cttgagaagt catacatgtg ccatttcgag
    28921 cgattcgagt aggcaagaca cagtcaaaca tgtctactcc acggataaca ccatcgatca
    28981 atgaatcagc agcaccgaca cccatcaagt aacgtggttt gttttcgggg atcaacggtg
    29041 tagtgaattc taatacacga ttcatttctg atttaggttc cccgacagat aacccgccga
    29101 ttgagtaacc agggaaatcc atcccgacta gatctttcgc gctttgacga cgaagatctt
    29161 caaaccctgc tccttggatg attccaaata atccttgacg gtctggatta gcatgagctt
    29221 tcaatcctcg ttctgcccat cgagacgtac gttctactga ttttttcacg tagtcgtaac
    29281 tttcatcgaa tggtggacat tcgtcaaaac tcatcatgat atcagagccc aatttatttt
    29341 ggatgttgat tgctttttct ggtgataaaa acatacgcga accgttcaag tgatttttga
    29401 aatgcacgcc ttcttcttcg atattccgca tatcacttaa tgaaaaaact tgaaagccgc
    29461 ctgaatctgt taaaatcggt tgatcccagt tcataaattt atgcagtccg ccagcttctt
    29521 ctactaagtc ttcaccaggg cgtagccata aatgatatgt attacttaaa ataacgccag
    29581 cacccatttc tttcaactct tctggggaca ttgtttttac tgtagctaat gttcctactg
    29641 gcatgaacat tggtgtgggg aaagttccgt gtggcgtgat caattcccct aaacgcgcgc
    29701 cagtatgttt ttctttcttg attaaacgat agcgaatggc aggttgcgtc attcatttcc
    29761 ctactttcta tataaaatat cttgacaact cccaccatca taatcgtttc aagtgctttt
    29821 tgcaagttcc ctcggcttcc ttctacttta tttaatattc ttttttttat ccttttttta
    29881 tgaataacaa aaaaaagaaa aacattatga cagaaatatt ttttgataca tggtatattg
    29941 gtgttattaa agaaaaggag ggaaaaatca tgcaatcttc tgcagaatta aaacgccaag
    30001 caaaagagag tctaaaagga cgttggggac aagctgtttt actaaatcta atccctacat
    30061 tgattggtat tgctactatt cttcttatta tgattcctat tatcttgttt gccaccacca
    30121 tatatgatgg atcagcgagc gtctcagaag cgacaacttc aggaggcggt agttcaggag
    30181 gcggtggcgg aattatttcc agcatcatta gcgctttgtt catgagcggt atttcttgga
    30241 cttatcttga tattttacgt ggacaaaaag atcagatcga accattcaaa gatgcatttc
    30301 gtggattcaa aggtttgttt atcggcggag tgatcagctt agctgtgctg atctcgatct
    30361 ttacaacatt atggggtttg ctattagtta ttcctggaat cattaaaagc tattcttatt
    30421 ctcaaagcta ttttatttac tacgatatcg tccaagaaac aggacaaaca cctaaagtac
    30481 tggatacgat cactgcaagt cgaaaattga tggatggtta taaaggacaa cttttctggt
    30541 tagatctttc atttatcggc tggcatatct tagcattagc aactgctggt attggttatc
    30601 tatggctcag cccatacatt tctgcaacaa aagcagcatt ttatgaacaa ttgccaaaag
    30661 atatttaaaa gagagtgtga cttgtgaccg agggggtggc aggagaacac cgtttatcga
    30721 gaggcagtaa aagaaacatt cagcccatta gcaaaaagga ggtgtgacaa cattcatgtc
    30781 acacctcctt tttgtatagt tccagtaaaa agaaaatcaa cttagaaaca gttaatcatt
    30841 cctcttcttc tgcttccggt aatcgatgga acttgatcag ttcattcacc gcgattggta
    30901 cgatagataa aatccctacc aagaaccagt gattcaaact gatgtgtact aggccgaata
    30961 gttcttgcgt aaatggcagc aaagcaacag ccgttgtaat agctaatgcc agtaacgcca
    31021 tttctaccaa agctttattg gtcgctaaat tgactttgaa gatcgattgg ctgctgcgta
    31081 cattgaacac atgcagaatc gatgaataag ctaagatcaa gaatgcaaca gtttgaccga
    31141 catgctgact tgcgtctacc catgggctga tagtatcgac ataactacct aaataataac
    31201 caaacaaggt tacgatggtg aagacccctg catttatggc aattttttgc aataaacctc
    31261 gagcaaagat cccttcattt ttcggaatgg gtggctgatc catgatgccg tgttccgctg
    31321 gctctcttcc taaagcaaat cctggaagtc catcagcaac tacattgaca aacagtagtt
    31381 gcacagctgt aaatggtgct ccccatccta acagcatcgc aatcaaaaca ataaagatct
    31441 ccgaaatatt acagcttagc aaaaagttga tagctttacg aatattctga taaaccgaac
    31501 gtccttgact gacagcgtct acgatcgtcg caaagttgtc atccgtcaaa atcatatctg
    31561 cggcactttg cgccacatct gttcctgtga tccccatggc acacccaacg ttacttgctt
    31621 tcaacgctgg tgcatcattc acaccatccc cagtcatcgc tacgactgca cccgtacgtt
    31681 gccaagattt gacgatccgg atcttgtctt ctggcgttac ccgagcatag acagagagag
    31741 ctttgacacg cgcatctaat tcttcgtctg ataattgatg cagttctgat cctgaaagtg
    31801 cttctgacgg atcattcaag atacctaatt ctttcgctat ggcactggca gtcaccacat
    31861 gatctccagt gatcatgact gttttgatgc cagccttttt cgctcgcttg attgctcctt
    31921 tactttctgg acgtggtgga tcgatcattc cgatcagacc aatcaaacgt aagtcttttt
    31981 ccagtgcttc agaagaaata tcttgcggtt cttcgtcata agtagcataa ccaacagcaa
    32041 tcactctcag tgcccgttta ccaaagcgat cattaacgat cgccgcttgt tctacatcgc
    32101 ctgaactaaa gcgcggagca agaacatcga atgctccttt ggtgattgag atgtattttt
    32161 cgcccatttg gtgaactgtt gtcatcattt ttcgttcaga atcaaaagga agctcgttta
    32221 ctcgtggata tttttcttct aattctgcct ttgtatgata attctcttca acagccctta
    32281 cgatggctac ttctgttgga ttacctttga taacaaggtc gtcatcttct tgctcgaccg
    32341 tcacatctgt acatagtgca gccattttca atatttccat cgcgctgttc gtcattgaat
    32401 cttcgatatt cgttacttca tcttctttgc tccatacccg gcggacgcgc atttcatttt
    32461 gtgtcagcgt tcctgttttg tctgagcaga taacattagc ggttcctagc gtttcaactg
    32521 ctggcaactg tcggatgatc gcatgtttcc tcgccatctt ctgcacaccg tatgcaagtg
    32581 tcaacgtaac aataacagtc aaggtttccg gtacagctgc aacagccaag gaaatcgaag
    32641 tcatgaacat ctcaaggagt ggttctcctt gcagttcacc aatcacaaat actaaagccg
    32701 cagccgctaa agcgatcata ctgattcgtt tgcctagctg attcaatcgg acctgtaaag
    32761 gtgttttttg catagtattt tcgttcaaaa gatccgcgat cttacccatt tctgtagcca
    32821 tgccaatggc agtgacgatc gctttcccac gacctgcagc aaccgtacat cctttaaata
    32881 ccatattaag ttggtcacct aatgactcat cttcttcagc aataaatgct gcatcttttt
    32941 caactgcttc actttctcca gtcaacgccg attcttctac acgtaattgt gatgcttcga
    33001 tcaaacgggc atcggcagaa atagcatcac cggactctaa aacaagaata tcacccacta
    33061 ccagttgctc tgactcaaca gtggtcactt tgccgtctcg tatgacagtt gccgtttgtt
    33121 tattcatatc ttgcaaggat tccagtgctt tttctgcgtt tccttcttgg acaatcgcca
    33181 ataccgagtt gatcacaacg atagcgatga tcagcagtcc ttcaaataaa tccccatgtt
    33241 ctgtagcaaa tgcggtataa aaggaaatca ccgctgctac aagaaggatg atcgttgtaa
    33301 aatcggacaa gcttcgaaag aattttttga tcatcgattc tttcggcgcc tcatcgaatt
    33361 tattcaatcc ctgctttttt cgccgttcat ccacttcttg tgaagacaac ccagtatctg
    33421 gatctgcttc tgttaccttc tttacttctt ctattgtctg cttgtattct tccatcctat
    33481 tcctccttta aaatcttcgt acaaaaaaag gtacttattt tactataaca tccttttttt
    33541 atttacttca aataaagggc ttatcgatta tttgttaaga aagcgtaaaa tttaaagtgc
    33601 ttatatttgc aaacgctacc aaaggatgct attatataga tgtaaggaag gaggatacca
    33661 tcaaatagac agaagaattt ctcgatagaa cagtgtaagc aaacaaagtt gttagaagta
    33721 caggtttcac ccagacatgc gtgtagagta caaacctata aatagtgaat gcgtatggat
    33781 gtttggatca cgaagtatcc gaaacaagaa gtaatgcgat acgtaacaat tgaataacgt
    33841 aaacgtctga aaattgaata ggaacaagag atgtttcaaa tgttgaagat gtaacgaaaa
    33901 caaaattttt gtttaacatg aagaaattca acaccgtgct ttgcatgtat gatggaaagt
    33961 tacaatcatt tctgtttatt tgaatccttc cgaggtacaa gaaagccgag cttatcgaga
    34021 gctcggcttt ttctttgtca taaaatcttt gtaaagcttt tttgttattc aaaactagcg
    34081 gcttttcata accttatttc acgaacatcg catctccaaa actaaagaaa cgatagcgct
    34141 cttcaatcgc atgctggtaa gcagataaag tcaattctct tcctgcaaag gcactaacta
    34201 acatgactaa agtcgatttt ggcaaatgga aattagttga gaaagcttct accactttga
    34261 attcatagcc aggcgtaatg aaaatatcag tccatccgct gtctgctttg atttctccat
    34321 taaatttcgt tccaatcgtt tctaatgttc gaattgaagt tgttccgaca gccacgattt
    34381 tcccgccatt tttacgtact tggttcaatc gctcagccgc ttcttccgtc aaacggtaga
    34441 attcactatg catatggtgt tcttcaattt tatctacgct gacaggtcgg aatgtaccta
    34501 accctacatg tagtgtcaga tagactaatt caacaccctt tgcttcgatc tcatcaagta
    34561 attctttcgt aaaatgcaat ccagctgttg gcgctgcagc agaaccattt tctttcgcat
    34621 agacagtttg atagcgatct gggtcttcca gacgttcttt gatatatgga ggcaatggca
    34681 tttctcccaa tgattcaaga atctctagaa aaattccttc atactcaaag tcaatgatcc
    34741 gtccgccgtg ttccaattct tctttcacta ctgcttgcaa tctgccatca ccaaaagaga
    34801 ttcttgtccc aactttggca cgtttcgcag gtttgatcaa tgtctcccac gtatctcctt
    34861 gtgtattcgt tagaagaagg acttctaaat gcgcaccagt atctggtttt tctccatata
    34921 aacgagcagg cagaactcgt gtattattca taactagcgc atcaccaggc tgtaactctt
    34981 caatgatttc atggaaatgt ttatcttcca tctctccagt tttccgatcc aaaacaagta
    35041 aacgagaatg atcgcggtct tttaatggtg tttgtgcgat taattcttcc ggcaagtcaa
    35101 aatcaaaatc ttctgttgtt aacatatcgt cactcacttt ctatcatgtg cttcatcatt
    35161 ctatcatgtt tttcgttctg ttggcaaaat cagcactttt tcacttctat tttttgtttt
    35221 cctaaataaa aaaactaccc tatttgtatt ttttcattgt tttaggcata ctagtaatag
    35281 atagttcttc attgaaaata gaaaaagagg tggcaccatg attgaatttc aaaatgtgtc
    35341 aaaaatttat aaaggtggaa aagtcgctgt agaggacgtc aatctttcat ttgaaaaggg
    35401 tgagttcatt tgttttatcg gtacgagcgg aagcggaaaa acgacgacga tgcggatgat
    35461 caaccgaatg actgatccaa cacaagggaa aattttgatt gatggaaaat ccatccaaga
    35521 aattgatcca gtggaattgc gacgtcagat cggctatgtt atccaaagta ttggattgat
    35581 gcctcacatg acgatccgtg aaaatatcac tcttgtccaa aaattgttga aagtcagtca
    35641 agaagaacgg aataaaacag ctgaaaaaat gattgatctt gtggaattgc cccgtgagat
    35701 gttagatcgc tacccacatg aattatctgg tggtcagcag cagcgtattg gagtcgttcg
    35761 tgcccttgca gcgaatcaag atatcatctt gatggacgag ccattcggtg cgcttgatcc
    35821 aatcactcgt gattccttgc aagaccttgt caaagattta caagagcgtt tgggaaaaac
    35881 gatcgtattt gtaacccatg acatggatga agcgattaaa ctagcaagcc gtatcgccat
    35941 catgagtgaa ggccgtgtga tccaatttga tacaccagaa aatattttac gtcatcctgc
    36001 aaacgatttc gtagaagagt tgattggaga agatcgccta ctacaagcca agccaaatgc
    36061 gacaagagtc ggcgaagtta tgctaaatac agcaatcacg attacaccgg aaaaatcatt
    36121 acaagaagca atcaaactga tgagggaaaa acgtgtagat acgttgttag ttaccgataa
    36181 ttcgaatgta ttaaaaggat ttatcgatgt agaaacgttg aataaaaaac gaggaaaagt
    36241 atccagtgtc ggcgacattt tgaataaaca tgttttttac gtaaaagaat ccgcctatct
    36301 tcgtgatacc ttgcaacgca ttttaaaacg cgggctgaaa tacgtgccgg tcgttgatga
    36361 acagaacaaa gtcgtaggta tcctgactcg tgcatcatta gtggatatcg tctacgatgt
    36421 gatttgggga gaagatcccg caactactga tgttgcagag tcagctactc ctgaagaatc
    36481 taaggaggtc tgacctatgc agcagttttt tcatcagcac ggttcagaaa ttctctcaaa
    36541 aggttttgaa catctttata tttcaggact tgctttgatc attggtatca tcattgctgt
    36601 accgcttggt gtcttactga cacgttttcc aaaagctgcc acgattgtga ttggcttaac
    36661 cagtgccttg cagacaatcc cttctcttgc tttattggca ttgatgatcc cgctatttgg
    36721 cgtaggtaaa ttacctgcga ttatcgcact ctttatttat tcattattgc cgattttacg
    36781 taatacgtac attgggatga aaaatgtcga ttggaattat cgggatgtgg caaaaggcat
    36841 ggggatgact gagttccaat ccattttttc cgttgaattg cccattgcta tgccaacgat
    36901 catggctggt attcgtttag ctgccgttta cgtgattgca tgggctacac ttgcctctta
    36961 tatcggcgct ggtggtttag gcgatttgat ttttagtgga ctgaataatt accaaccaga
    37021 tctcattttg gcaggtacga tccctgtaac aattttagca ctattggctg attggcttct
    37081 aggtctcttg gaacaccggc tgacaccgat cgcacttaga gaggagaatg aagaatgaaa
    37141 cgtatactaa aaatgaccat ggttcttttc tctcttctta tacttgcgag ttgttccttc
    37201 cctggtcttg ccagcaatac agatgaagac accatttcga tcacaggtgg aatcacttca
    37261 gaagcacaga tcttagcaag tctagttgcc ggaatggttg aacattatac agataaaaat
    37321 accgcaatca tcaacaatct ggctactaca actatcaacc atcaagcgat gatgaacgga
    37381 gatgcttcaa tttcagcggc tcgctacaca ggaacagatt taacgactac attgaacctt
    37441 cctcctgaaa aagatccgaa aaaagcgttt gctaccgtga aagatgaatt tgagaaaaga
    37501 tacggtcaaa cgtggttccc gtcctatagt tttgaaaata cgtatgtctt tcttgttcga
    37561 aaagacaccg cacaaaaata ccatctatct aaagtcagcg atctgaaaaa tgtagcggat
    37621 gaattagtag ctggtgtaga tacttcttgg atcaatcgta aaggagacgg ttatgacggc
    37681 ttccaagaaa cttatggctt ctccttcaat tcgatattac ctatgcagat tggacttgtt
    37741 tatgacgcag tcgaagcagg aaaaatggat attgttcttg gctactctac ggatggacgg
    37801 atcgccagtt atgatctagt catgttggaa gatgatcgtc aattcttccc accatatgat
    37861 gctgctccag ttgtagacaa caagatatta gctgatacgc ctcatttaaa agaagcctta
    37921 aataagttag gtcataccat cagtacggaa aaaatgcagg aattgaacta cgaagcagat
    37981 aataatctag tggaaccatc agtagtagca caacgtttct tacaagaaaa ccactatttt
    38041 gaagggaagt gaagcaattg gataaaatga atgtctttca acaatttttc tattatttcc
    38101 aggaaaatgg ctcctatatc tttgcacaat ttatccgtca cttcctgatt tctatctatg
    38161 gtgtcctctt tgcagcagtt gtcgggattc cggttgggat catgatttct aggagacgta
    38221 aattagcaaa ctgggtgatt cgtttggcga atatcatcca gacgatccct tctctagcca
    38281 tgatctccat tttaatcatt ggattaggcc tgggagtcaa tgtcgtgatt gttactgtat
    38341 ttttgtattc cctcttaccg atcatcaaga atacgtatac cgggatgatc caagtagaca
    38401 aaaacatcct tgatgtcggc aaaggcatgg ggatgactgc tagacaacga ctattcatgg
    38461 tagagttacc actctctgtt tctgttatca tggcaggcat aagaaacgcg ttagttgttg
    38521 ccatcgggat aaccgcgatc ggtgcattcg tcggtgctgg tggtcttgga gatatcatta
    38581 tccgaggaac caatgcaaca gagggcacat cgattatttt agcaggtgca ttgccaactg
    38641 cattaatggc gatcatcacg gactggcttt taggtatttt ggaaagacgg ctagatccag
    38701 ccagccgtac atcacgctga tgttattgta aaaaacaaaa gcttctgcga ccatttgttg
    38761 gtcgcaaagg cttttttagt tctgtcattt ttaaggcaat aatccactct gattcttccc
    38821 tccttatggg taaagagttg cttttcttag ctatttatcc attccgtata ctccaacttc
    38881 atactgccaa cgggttcata tctgcatcca ccgactaagt ttttttctta tccgctaagg
    38941 cattttgcca atccattttc ccatcactcg tcatggaatt cataccacat gatctctaaa
    39001 tactcgtctg cttgttcttt gaattgggca tcaattctta catcctttgg cgtggctttc
    39061 atttaagcct tttccatcag tcctcgattg ttcgatgcct tcagctgcat catcaaaact
    39121 ggtgactgcc gtatcggtaa cttcgatcat cttttataca atcgggaact tgccatcctc
    39181 atccttggct aaaaacatat ctagccattc cagcttctcg ccatgggagg tttgcggttc
    39241 ctaaaaccgg tacacgattc ttatggactg catccgtcgc aactagactt ggcgatacga
    39301 tgatccttca ccagaagagc acccccgtaa accaattgat cgatatattg ccaataagaa
    39361 aagacattgg cgtaaaagat atcgatccct cgtgatgcat tgccgttgat actgctattc
    39421 atggccgaaa gcgccacgac tttaacttct tccgtttgag tgacattcat gttcggcaat
    39481 gtcgctttgg atacccgctc tgctaaagga acgacactgg tattgtattt cgtatctgta
    39541 tcttttccaa aagaccatgc caacgaatcc tctgggaacc aataagacga ctccggttga
    39601 ttgtccattg cttttttcaa catacttttg gctgtttctc gataaacgac atttccaatt
    39661 gtcttcgcct cactttggcg ataatcgatg gtcgttgcca ctgccaacac taacaacgat
    39721 gctccaattt tactgatttt ccttgtcaca cttcttcctc cttgttttac ataaacccgt
    39781 caacattcca ttcctaaact caaacaatga cccttcaaag gtggagatat aggaacaaat
    39841 taaagaaatc atgcaataaa tccaagaagg ttggttttag aacaaaaaaa gagtctcctt
    39901 tttggggaga ctctttgtta actatctaaa aaggttggct cttctcactt cactcataat
    39961 gtgtccaggc agaataaatt tctacaactt tattttcctc atcaactttg taaaccactc
    40021 gatgctgtct attcagcctg cgtgagtatc tgccttcatg tgtaggtctc agcttttcaa
    40081 aagactgcgt gggcatatat ggatcttcct tcaatgtctg aatcacttct tcaaattgtt
    40141 tctttaaatt cgtttgcttg atttttctta aatccacttt cgcagagttt ttgattgcga
    40201 ctgtatagtt actcatcaaa gagcttccca atcaatatca tcgatattcg taaagctact
    40261 gtcgtctttt tcgcggtctc gtactttatc taaagtacct gtttgctcaa gaaaaagggt
    40321 ttcttgaatc gctctccagt cttccagccc aatcaataca gcattgtttc cactacgatc
    40381 actaataatc tcgatttcag tatgattttc atttacctct ttcaataact gataaaaatt
    40441 tttacgagca gtgctaggat tcatcgttgt attagccatt gtctctcctc ctttttctga
    40501 tataattata gcgtacttga ttaagtacgt caaaaaaata caataaagca cttaaattca
    40561 gcaaactttt cctctttttt acatagagaa aaagacccct ttttagggag gatctttgtc
    40621 cacgctcact tcttactcct ttacgactag cggtagccaa acgtaaagta attggtaaca
    40681 acgtatcatc tttagcaaag gtcgaatcca aagcctgtga gtctatgcaa caacaccaat
    40741 acacgaaggt tcctcgttgc agtgtgactt gattgatatc ttgaataacc tgaggatacg
    40801 cacgaaagat ttgtaacggt tattcaaaac tgattttggc cgtcaagtct tctactgcct
    40861 caaaaatgat tgcctctttc gtctcctgtc attcatgttc actatcgatt tgatacttgc
    40921 ttagaccgtt gggtttgtga atcaccaatt aaaaatggca ctgagttatg gtaacaaaaa
    40981 atggtcactt gacgaggctg agattcctac ttatgaagac acatggcact taagagtcga
    41041 ggtaaaggaa gaagctgaaa atataggagg ctgtacatta tttggcaaag gattcggaaa
    41101 ttatgatcaa gacatcaaaa tcaaaatgtt ttacagctaa ataggaagaa taataacatt
    41161 tcatacgctg ttcccatctt taatggctta tagagggcac ctttagctga gaataagaca
    41221 aaacagaccc tctagcttca attaagagct agagggtctg tttttgattt taaaacttgg
    41281 tttatttctt caaatggtag ctataggtgc tgacaacgat attttcacgc caagacaatc
    41341 gcaatcttaa ccgcaaactt gtttggttat ggagaatatt ctgccagcgt ctgtttggta
    41401 caaattgagg aatcaacaca gtagttgtat agttttgttt cgctgcattt ttacttacga
    41461 tatctacata acggataatt ggattttcaa tcgagcgata agaagagtgg acgattgaaa
    41521 aacggacatc tgggaaatgc tttttaaatt ctgcttcgat ttctttttct ttttcgacat
    41581 cttcgtctag cgaaacatgc atcgcaacga cataatcacc gatcgaacgt gcgtaattca
    41641 acgcaccgat atttacccgt gtcacattcc cgactaatac aagaacggta ttcccgtcat
    41701 aatcatgtag ttttgcatca ttttctaaac gaagctgttc tgctactttt ttataatgat
    41761 catggatctt ataaaaaatg aacataacga ttggcataat gatgaagaaa ggccaaatgt
    41821 cccctaaccg atagacaaac aaaatcgcaa tgattgcata tgagatgaac gcacccgtaa
    41881 tattggcaat cgatttgctt aaccatcttt ttccttcttt tttccatttg acgaccatcc
    41941 ctgtttgaga taaagcaaat ggaatgaaca cgccgataga gtataacgga atcaagcgtt
    42001 ctgttgagcc atggaaaata aacagcaata cgatcgaacc tagcgctagt gtgataatcc
    42061 cattggaata gcctagtcga tccccacgat cttgatacat gtgaggcatg aatttatctt
    42121 ttgccagatt gtatgcaaga actggaaaag cagaaaaacc ggtgttagca gcaactgcta
    42181 aaatcagcgc tgtagcaaat tgaaggacgt aatacaaaat accatgtcca aagacagctt
    42241 taccaatttg agacaaaaca gtgacttctt tttcaggaac gatcccatac caatagttga
    42301 taaatgtgat ccccacgaag aagaaaccta agatcaatgc catcatcgtc aatgtagcag
    42361 cggcattttt tgctcttggc ttcttaaaga atggtacagc attactaatc gcctccaccc
    42421 cagttaaaga ggaagaacca gaagaaaatg ctcgtaaaat caaagcgata gaaacacccg
    42481 gcacagccgc tcctggaagt gcagtagctt gcaatggcac agcacctgtc acgatattat
    42541 ataaaccaac aacgatcagt aacgagatca caaggataaa tgtgtacacc ggaaataata
    42601 ggaacgatgc agattccctc agcccacgaa gattcagcat catcagtagc aaaacgattg
    42661 tcacagaaat agcgacttga tgaccgtaga gtgctggcac cgctgatgtg atcgcttcag
    42721 cacctgctga gacagatact gctactgtca acatgtagtc gatcaataaa gagccaccgg
    42781 aaatcaagcc agcattcttt ccaagattct cactgctgac gacatatgct cccccgccat
    42841 gaggataagc atggatgatt tgacgatatg atagcgttaa tgaaatcaat aaaataataa
    42901 caaaagcggc aatgggtagc gaataccaga tggctgcagc agacaaagcg actaaaacga
    42961 caacgatttg ttccgtacca tatgcaatag atgataacgc atcagacgac aataatgcca
    43021 aagcagcaaa tcgggttaat ttgtgctcat cattttcagc agattttaaa ggcttaccta
    43081 ctaacaacct ttttaagtaa tccaatttct aaactccttt acacacaatt ttacttcaag
    43141 ttttatagtc gaaaatttat agtaaacaag atgttatagt acgctcactg accttaaaag
    43201 tcaataaact ttaagaaaat aattggaaaa aaacagaaaa atatcaagcg ctttttcaaa
    43261 cgctttaata cagcaaaaat ctaacatttt tttatgggct ttttctttga attctataat
    43321 aaaaggatag aacttgactt agaaaatcaa gttctatcct ttttgatatc ttaacttttg
    43381 gaagagagat gtaaggctgc tttctaccca tcattcttac tactcgaagc atagcgcagt
    43441 tgtcacaacc tcttgataaa cggtgtttaa aagcttgcat ctgcggtaat aagcccaacc
    43501 aaattaaaaa cataagaagc tgtttttatt tggtcgttct tattacttga tgcagagcag
    43561 cttttttaca acctcttggt acacggtgtt caatcagctg accctcggta ttagctcaaa
    43621 aaaacggcaa aatatgagga actatttttc catttttttc tctaatactc aggtcaaaac
    43681 actgatttca caacctcttt ttttaatgaa aaatatggct ctcatgcgcc gcttctttga
    43741 atttcgtcat tttttcttcg atgaacggat agattgccgt tcctacgatt ccaaagacaa
    43801 caaacaaacc gatcatgatc cagattgctt tcgtagcatt tggccaataa attcctcctg
    43861 cagattcccg taacaagttt accgcatgag taaatggcaa gaacggattg atcatttgga
    43921 agaatttccc tgagacttgg atcggatagt tcccgcctcc gccagagatc gacagtacta
    43981 agatgataat agcgatccct ttaccgacat tatcgaataa tgcagccaag acatagacga
    44041 tcatcatgaa cgctaaggcg atcaagacag caaatgacac gctgtagacc ggattttgga
    44101 catctactcc tagtaagaaa atattcccta atgtaacgat caagctttgc gcaatcgcca
    44161 ttgtcaaaaa tgtcagcata cgagcaacga attgttctcg tttcgtaaaa ttgtttcggt
    44221 ctttctttcc taaatagtat tctgtcgtag caacacttga aagaagaaca gcgccgaccc
    44281 acagacatag tgcagtataa aacggggtgc tagctgaacc gttatttgcg attggataaa
    44341 gcgcattggt ttgtagctca acaggttgtg taaagaagtc actttctttt gttgcgtcta
    44401 gtttcaataa tttgatgact tcgcctagat cgacttcttg ttcgcctttt cggatcgcgt
    44461 ctgctgcttt ttggatacca gctttgatat ttggccagtc attgttgatc aagtccgcag
    44521 cagcgttcac agctgtttcg acttctggca ttttttcatt tactgttgtc aatgtaccgg
    44581 tgatttcatt ttttagtgct ggccaatcat tttggataaa atcagcggcc aatcctaatt
    44641 tttgctgaat gatcggtaat tcattgttgt ataggtctgc cccttcattg atgccattga
    44701 cgatggtatc catatttcca ttcagcattg tattggcatc atggatttct tgtccaattg
    44761 caggcatttc tttttgatat ttttctaaaa ttgccactgc attcgttaca gttgctttcg
    44821 ttgagctgag aagcgaatca aagtcgatct ggtccgcttt gctcagtgcg ccttgtgcag
    44881 tcccaatcgt atcgatgact tggtctagta gagaagtgac agtggaactg atttgacctg
    44941 tattgatacc agcaatgatg ccattgatat tattaacaac ggcatcaatt tgatctagca
    45001 attcatcaat agccttcgtg tcttcatttt gaacagctgt gttcaaagca gataggcggt
    45061 tttgcagatt gcttagcaag ggttttaatt gatttagctg atcaataatt ggctgtaatt
    45121 tcgtatttcc agcacttgtt tgcaatttag tcaggaactc gaccagttta tcgattgcat
    45181 tttgttgttg agatattccg tcaataaagc cgttcaatac attgctgatg tttttctttt
    45241 catcatcagt cagcttacca tcttcaatag ctgttcttac agtatctatt aacgaacctg
    45301 tcgttgttgc cacttgtcca attgacttca atgtaacatc tacactgttt gtgatgcttg
    45361 gtatcgcatc tttcagctga gaggcacctg attttgtttg gtttgctaga tcacttgcct
    45421 gattttctaa atttttcaca tctggcaaga tatcttgtac ttgttggatg atcgtcaagc
    45481 cttgttttgc ttcatcgatt ccttggttca ttgtctcttg gatcgaagca aaatcgccgt
    45541 cgatctctgc tagttgctta ccggcatttt ggatctctgg gattttttct tgcaaatcaa
    45601 ggatgatttt tgcttgatct ttcaactcag gcattttgtc attcaatgca acgacttttt
    45661 cacccatctc atctacttta gggatgtaat ccacgaattc atttgcttta ttcagttttt
    45721 ccttgatttc aggtagttga ctttgtaatt cgaggacttg ttttgtgtag ccgtcgatcg
    45781 tgtccaaatt ttcatctgtt gataaaatca tatctttaac tttattgata cttactaggt
    45841 tcgtatcgat gtcgtaacct atctcattga atactttcaa taaagtcgaa ctggctgttt
    45901 tgataaattc atttgtgatt tgcgattgga tcgaacttgc tcctttatcg gtgattttcg
    45961 gtgcaatcgc attgattttt tcatttaccg tatattcgat ttttggtttc ttgatatcac
    46021 cactagtaaa actcagcaga tcttctgaga aatcctttgg caagtaaata cctgcgtaat
    46081 actttccgga acggactccg tcttctaatt gttcttttga atcaacgaat tgccatccta
    46141 attgtttatt tttatgaagc gattcgatga cttgctcccc aatggccact tctttacctt
    46201 gaaactctgc tggcttgtct gcactgtaaa cagcaattgg cagctctcca gtatttccat
    46261 atggatccca taatgctttg atgttaaacc acgcatatag cgaagggatg atcatgatcg
    46321 cgatgatcag aagtgttgca atcgggtttt tgaaaatccg tttccagtca agcaaatata
    46381 atcgaagcgt atttttgatt gactgcatag ttctccccct tcttcttttt tcagctgaat
    46441 tactttacca tgtatttcat acttttttca atcggctttt ttcaaaaaag tcgaattttt
    46501 ggacatatca cggcaattgt ctcattgtaa tctcttacat attgagaaat aattaaggat
    46561 tacagccttt actttatcat gaaataaaag ggaaagataa ttttatgcca aagtttttat
    46621 ctattatacg tatacgcaaa aaagctaaaa cattttttgt ctttgcccct acaaaaatgt
    46681 ttcagctttt ttcgtttttt attcctgtat ttcttatttt tcgaatgaca ggatgatttt
    46741 cccagcagga ggatgtcctt caactagctg atgtgcttgg atgacatgct taagtgaagc
    46801 agacagattc attgaaataa aaacatgaaa ggctcctttt tggtaggctt tgattatgcc
    46861 agcaaaggct tctgcatcta gatattcctt tcttggaaca aacgattcat aaaagccttc
    46921 ctttttttgc cgcaaatcta gatctgggag gtcatttaat gcaacatacc tgccgcctgg
    46981 tttcatgatc tggatgccgg tttctccttt gatacttcct ttcgtcgcat cgattacaag
    47041 gtctgcctga tcagcaaatt gcagacctgg atttgtttta tcataagcag caaatgctga
    47101 agctcctaat ttttttacct tttcctcatt tttactggat gcactggtta agattcggat
    47161 tcccttttca tggagaagct ggatcagact tgacccgacc gcacctgatg ctcctagtat
    47221 cattacagta tctgtcggct ggatttccgt caaatggttg attagattat aagccgtaat
    47281 tcctggcgtg accattccgg ctgcttcttc ccaactcatt ttatctggaa tttttgctac
    47341 ttttgctgat ggcaacacga cttcttctcc gtaagtcccg ccgactgcat ggacagctac
    47401 tcgatcacct acatgaacag ttgtgacatc tgaaccaatc tctgtcacaa taccagcgcc
    47461 atcattacct ggtacataag ggaatttcaa tgttcgtatc tctttcattt ctcctaaacg
    47521 caaggcaaca tcataaggat tcacactgaa tgcttttatt tctacacgaa catgtccaga
    47581 accgacttct ctgggatgag catcgatttc ttcaaaaaca tcttgtgctt ctccgtaacc
    47641 gttaattgca aatcttcgca tggtcaccat ctcccttttt ctttaagttt acgtttatgc
    47701 ctttattttg acaaaaaaaa tgtcttttcg ttttggcgca tgattcaaaa caaaatagtt
    47761 gtcttttttc gcgtaacgtg ttaaattatt tatctaaaag cgtaaagatt acgatttacg
    47821 ctaataaaaa ataattggag gaatttgttg atggctaaaa aatcaaagat tgccaaagca
    47881 aaaaaacagc aagaaacgat tgaacgctat gctgctttac gccaggaatt aaaagcgcaa
    47941 aaagattacg aaggcttgcg taaacttcct ttagatgctc gtccagaacg attgaaaaat
    48001 cgtgatttaa tcgatggacg ccctcgcgga tacatgcgta aattcgggat gtcccgtata
    48061 aaattccgtg aattagcaca taaaggtttg attccaggag taaaaaaagc cagctggtaa
    48121 tccaagtctg gctgtaatgt aaaggaggag ggcgcatgga ccactccttg gatcatcaaa
    48181 aaagatacgt tcgcttcttc tagcagaaat gctggtacta gaagcagacg tatctttttt
    48241 tacgcacagc tggtcctttt ttatatgtaa aggctgcatg gttacttcgc tatatttatt
    48301 tagtaacagg agtcagtgct tcgataccca cactaatttt caatgctcgg tcaacttctg
    48361 ccataatggg tcgatccaaa tggcagacct tttctttcaa tcttgattta tcgatcgttc
    48421 tgatctgctc caacaggata acggagtctc tttctattcc tgttccagta gctttgatcc
    48481 caatgtgagt cggaagctta ggtttagcca tttttgccgt tatggccgca acaatgatcg
    48541 tcgggctaaa atggttacct agattatttt gaataaccaa aactgggcgt gttccgcctt
    48601 gctccgatcc cacgactgga gaaagatcag caaaataaat atctcctctt tttaccatag
    48661 gatcctcctt attcttgata ttctcttggt atccgctgtc ctaatccgca agccacttca
    48721 tagtggatcg tacctaattg atctgctata tcctgcatag tgatttcttt gtctgcattt
    48781 ttgccgataa gcgtcacttt tgttccgaca ggaaattctt gaggcaatcg gatcatcagt
    48841 tgatccatac atacacgccc aatcgtttca cagtagttcc cttctaccaa tagagaaaaa
    48901 ccctgcatct ttcgtggcca gccatctgca taaccgatcg gtatcgttcc gatccactct
    48961 gcttcaggag tgatatatgt ttctccatag ccgattcctt cacctgctgg gagttttttg
    49021 acttgaatca attcagaaac cagctcaagt gctggctgca atggataaac ttccggtagt
    49081 gcgtgcccag agggattaag tccgtacatt gctactccat aacgaatcat attgccgatt
    49141 gtttcatcat gccataaagc cgttgcacta ttgctgacat gtacatatcg aggaagttcg
    49201 ggaagttttt tcagcacttc tataaagcgt tctttttgca gattccaata tgtatcatct
    49261 gcctgatctg ctgtagaaaa atgggtaaat aaaccttccc attctaatac acgactttct
    49321 ttgatgaatg cagctgcctc ttttacttct tcaggtgtac aaaatccgat acgacccatc
    49381 ccagtgtctg cttttagatg aatcgaaaga ggcgtctcta gcttactttt ctctaaaaca
    49441 gcagctgctg cttgcaacca ttcccgtgta cctgcagtta ctgataaatc atgagcaaca
    49501 gctacagtca gtgcgtcgta aggaaccata ttcaagataa ggatgggctg agtaaatcca
    49561 gcttctctca actcgactcc ttcatctaaa tttgaaacac aaaagcccga agctccacct
    49621 gcaactgctg cctcagctgt ttcaatagct ccatgaccgt atccgttcgc ttttactact
    49681 gcaaacaatt cttttccttg cggaagtctt gcaacttcat ttgctacatt ttcagttata
    49741 gcttttttat gtatcactgc tttcgttggg cggtgccaag cgacaaccat cgtttttcct
    49801 tctttctagt cttccaaaat aatttgtgca aaagctgttt catttgtatg agtaatactg
    49861 acccatacat tgccttcatg aggtgaacgc gtcacaacag gctgaccatt ttcatttttt
    49921 aaaatctcga catcttgaaa ggtaactttt ccgatcccag ttccccaagc tttagagaaa
    49981 gcctctttgc aagcgtaacg cccgcctaaa aattctactt ggcgatgaaa aggcaaggac
    50041 tggaatagtt tcatttcatc agaggtcaag attcgtgcaa tgaattttgg tttttcttcg
    50101 atcagccgag tgatcctagg aagttcgact gcatcgatcc caattccttt tatcatatgc
    50161 ttttctcttt tcaataaatt tagaacactc tcattctacc aaattataac cattttgcca
    50221 caaataagtc cacaaataac aaaaatgaaa gtttctctta aaaaagagaa actttcattt
    50281 tatgtttaat cgttatttga acggattaca aatccgcgtt ttttatcatt gtgacgttta
    50341 ccgccatctt tttggctgtt attatttttg ctataaccgc cttttgattt gtttccacga
    50401 taattaccgc cgtcacgacg attacggttg tttccgccac cacgtttgcc atttttattg
    50461 tatccttttt tctgcattgg caatgggcgt tctggtgtga tcttcactgg cactgcatca
    50521 gctggatctt ttgctgtcgt ttttagcaaa agtgcaacta aatcttgtgc agaatattct
    50581 tctaataatt tatctgctgt ttgcaggtat ttatctagac cattttctgc aagttttgtt
    50641 tcgatttgtt caactgcagc acctaattga cctttgaatg cttctttttc ggttggtgga
    50701 cgtaaagtcg tcatccgttt tttcgtcaag ttttcaatca catgcaagta gtccatttca
    50761 tttggtgtaa cgaaagtcac agacataccg ccttttccgg cacggcctgt acgtccgata
    50821 cggtgaacgt agctttctgg atcttgaggg atatcatagt tgtacacatg agtgacacct
    50881 gaaatatcta atccacgtgc agcaacgtct gtcgctacaa ggatatctaa atgaccgctc
    50941 ttaaatgaac gcaatacgct cattcgtttt tgttgagaca aatcgccatg gattccttct
    51001 gcacggtaac cgcgtgcttc taatccgcgt gccaattcgt ctacacggcg ttttgtacgt
    51061 ccgaagacga ttgttaattc aggtgtttga acatcaaaca agcgagtcat gatatcgaat
    51121 ttttcgtatt cttttgcgcg gacatagtat tgatcgatca agtcagctgt catttctttc
    51181 gctttgatct tcacatgatg tggatttttc atgaatttaa cgccaatatt cttgatagct
    51241 ggcggcatcg ttgctgagaa aagtaatgtt tgtctttgat caggaacttg tgaaatgatt
    51301 ttttcgatat cttccaagaa gcccatattc aacatttcat ctgcttcatc caaaactaat
    51361 gtttgaacgg ttccaagttt caatgtgtgg cggttaatgt ggtcaagcat acgtcctggt
    51421 gtacccacaa cgatatgtgg acgatctttc aagccacgga tctgacggcc aatatctgct
    51481 ccgccgtaaa cggcttgtac acgtactttt ttgtcgcgcc ctaaacgata taattcttct
    51541 tgtgtttgga tagctaattc acgtgttggt gcgataacta aaccttgtaa ttcgtgacga
    51601 tctggatcga ttttttctaa cattggtaat ccaaatgcag ctgttttccc tgtacctgtc
    51661 tgtgcttgtc caataacgtc ttttccagct aatgctagtg gaatcgtttc tgcttggata
    51721 ggcgttgctt cttcaaaacc agcgcgttcg actgatttca acaattctgg tgataattct
    51781 agttctttaa atttcaaatg aatcctccta tatggttgtt aactgaggat gtagcttttt
    51841 tgctaccccg tcgatttgtt cggtcaaagt gtattgtttc tttttgacag tggccacgca
    51901 cgtttttatc cattcttagc ccctgtggca ccaccgaaaa atcactttgc gaagtttagg
    51961 cattagaaaa gccaaacggg tcgcgttcag ctttatcatt attatgacat ctggcttcaa
    52021 caagttgttt ctatggcaaa accagtccaa tttctcttca tctttcggca ataggttatt
    52081 ctaacatagt ttttcgtttt ctgcaagttt acagattttt atttcatttg ctgaagttgt
    52141 tctaccactt caaataagcc catgctgttg ctgcctttta gcataatact atctgttgct
    52201 tcaagatttt gtttgatagc atcaatcaat ttattctttt ctttttcaaa atagaacaca
    52261 ggtagttggg ggtatttttg gttcaattct tcttttagtg catgcatttg ttccccataa
    52321 aggaaaatca tggcgaattt ctcaggatcg atatgttcac tcatttgcgc atgcatattt
    52381 aaagactctt tccctaattc cagcatatct gctaacacag cgattctgcg tccttttgtt
    52441 ggcagatttt taaaagtatc taataccagt cccatggctg ttggatttgc attgtagaca
    52501 tcgctcaaaa tagctgcacc atttcctgcc gttagccatt cggttctgtt ttgagtgata
    52561 gaaacatggg cgagcccttg gcgtatctca ggtatggaca gtccaaagaa gcgcccgatc
    52621 gtatacgcaa tcaaagcatt cgttacatta taacttccag gcagcgggat cgtaaatgtt
    52681 tctccttcta ctacaaaatc ggtttgtgct tttccttttg ctgtcacttc cgcatgaaca
    52741 tcgccatttc caataccgaa tgttgtgact gtttgtacta atgttgccgt taaaggtgtc
    52801 agcaacggtt catctgcggg cacaacgagt aatccgtctg atacaagtcc gtcagtgatt
    52861 tccattttgg ctttagcgat tccttctcgc gacccaagat tttctatgtg tgcttcaccg
    52921 atcattgtga ttgctgcagc ttttggttga gccataagtg aaagttccgt gatttcattg
    52981 gcgtgatcca tgcccatttc caaaacgatc ttttctgtat cttggggcat gtgcaaaatc
    53041 gtgtaaggaa gaccaatatt attgttgtaa tttccttgcg tcttatatgt tttgtatttt
    53101 tcagacaaga cagacgcggt tagatctttc gtggtggttt taccattgct tcctgtaatt
    53161 gcaacgactt ctgcgcctac tttttccaag taataggttg ccaattgctg catggctttc
    53221 aaaggatctg ctacaggaat aactggaaaa ttttttggag cttgttcaac aggcaagctc
    53281 caaaatgaag cagatgctcc gtttgtgatc gcttgctgga taaattcatg tccatctcgc
    53341 gtaccagcaa gaggaacgaa taaacttcct tcggttatct tccggctgtc aaattcgaca
    53401 ttggtaatct ttgtcagtgc agagcattcg ctgcccacgg cttgtgcaat ttcttgtatt
    53461 gttagattca ttgagatctc tcctaaaaaa ggttgtgaaa aaagcgtttg gcctaaacgg
    53521 cgggttcgca tgactctcac ttccaagtaa atttctctat tcgtatttag aagctagaat
    53581 gaacgcggtt acgcaatcac tccagcctct tctttatttc tctttattca tttttttcaa
    53641 aaaaagattg acgctgatgg tatcgattca ttcctaactg gatcagttct tcaatcaaat
    53701 caccgtattt caagcccata ttttcccata agagtgggta catactgaac tccgtaaatc
    53761 ctggcataga gtttaattca ttcaggaata attcattttt atttgtcaaa aagaaatcgc
    53821 accggctcaa tccgcttcca cctaacatcg tgtaagctaa cttcgcgtac tcttgcgctt
    53881 tttgataaac ttcttctggc acttcggctg gaatctgcat ttcgatttta ttattgatat
    53941 attttgcttc ataatcatag aatgctacgt cttttacgac ttcaccaggc aaagtcgtcc
    54001 gaacgtcttc atttcctaat acagcaactt cgatttcgcg cgcttcaatt ccttgttcaa
    54061 cgattgctcg agaatcatac tgataggctg ttgctaaagc attttgcagc tcttctcggt
    54121 tttctgcttt tgtaatgccg acactagaac ccatattcgc cggtttgaca aacatcggat
    54181 aaagcaaaga accttcacat tgatcaaata cttttttagg attttctttc cattgattct
    54241 taagtactgg tacataagga acttgcggca caccagcagc ttgtaaaata tacttggtca
    54301 tgattttatc catggcacat gcactggtca atacgcctgc gccgacataa ggcatattca
    54361 atgtctctaa gaagccttgg atcgttccat cttccccgtt tggcccatgt aaaactggaa
    54421 aaacgatggc tccttcttct ttgatttcgc ccggattgat cacttttcct gtaaagcctt
    54481 cctctgtctg tccacttggg tcccatgaaa gatgcaagac atctttgctg gcaggttttt
    54541 ctgttagtaa tggacctttg acccattgtc cttcttttgt aataaatacg agttgaactt
    54601 ggtaataatt ataataaatg gcatttaaaa ctgaaaatgc ggaaagaatg gacacttcat
    54661 gctctgcgct gcgtccgcca tatagtaaag taatcttcaa aagattgtcc tcctgtattc
    54721 taaatcgttc agtgatttac tctcatattt tagcacaaaa caaaaataaa cgaataactt
    54781 atttataaaa aaacgagcca gcttctaaaa gaacgagcgt catttatttt ttgtcagcta
    54841 gattatttgt cccgtttttt ataaaaagtt atgataaaga aaaggaggga tgatgatggc
    54901 gcaaacaaat ccacgcagcc gcttggcaga aatcattcga gtgttcatca attacaatgt
    54961 agtgccaaac tttgtacagc aaaaaaatcc cgaacaggta aaaaaagcct ttgaagaact
    55021 aggccccacc ttcatcaaga taggacagat gctttctgtt cgagaagatt tgctttcttc
    55081 tgccttcact caaacattca aaacattaca agatagtgta cctagcgaca cattttcaac
    55141 ggtcaaaaag acgatcgaaa cagaactttc tctttcatta tcagatatat tcgatgattt
    55201 ttcaaaatcg ccttttgcct ctgcttctat ggggcaggct catcgtgcca agctgaagaa
    55261 cggtgacgcg gtagtcgtta agatccaaca ccctaacatc gccgaagaga tccgacttga
    55321 tcttcaacta tttgaacgag cgatcccttt gatcaaatac attcctgaaa ctagcgtagt
    55381 tgatctaaaa ggggtactcc aagaagtaaa acgttccttg atcaatgaaa tggacttctt
    55441 aaaagaaagt caaaatggcg aacaattcta tcaaaagaac aatggctgga aagagatccg
    55501 cagccccaag atttatgacg ctttttgttc gaaaaaagtg atcgtgatgg aagaaatgtc
    55561 tggtaaaaac ttgaatcatt taatgaacgc cgaaaacaaa actgaaactt ttatcactgg
    55621 aattcaaaac aagcagctga aacaagaagt tgcaaagctt ttagttgaga actttatgaa
    55681 acaggtgttt gatgatggtt ttttccatgc tgatcctcat ccagggaatc tgctattcca
    55741 tgtactaaca aaagaagaac agacacaagc tagccgaaaa actgaaacag tccatgaaaa
    55801 agagttcggt tcctttgctt ttcgagcctc cacttctgca gaagaccctg ttgcgccata
    55861 taccatcaac tacatcgatt ttggtatgat ggggcatctt tctgcaggac ttaggcaaaa
    55921 actcacacaa gccgttttag cgctgtatac gaaagatgct tatcgaatcg aaaaagcggt
    55981 ccttcgactt tgtcagcaag aaggctcgtt tgatgaaagt cggttccacc aagaactgac
    56041 aagttttctt gaacagtatt atgattctcc tattgatgaa atcaacttgc aagaagtatt
    56101 cacacatgtc gtgacgattt gtcatcaaaa taatctgcag ttcgatcgtg atatcacgct
    56161 tttgctgaaa gcatttggga cattagaagg ggtgatccgc gtcctcgatc ctgaagtttc
    56221 cctcatggaa gtcgcttcgc cttttgctca acattatttt ctcactcatc tggatgtaga
    56281 agatacattg aaacaaagcg gattagactt attggaaggg atgaaggctg cgcctaagat
    56341 ccctcaacag ctccaccatc tattagaaat gtggacttcg ggacaaggaa aagtcaattt
    56401 ggaattgaaa aagcaagaca aattattgag caggatcgaa agtatgatca atcggcttgt
    56461 tttcggtatg attttagctg cattgatcgt agggtcctcc ctgcttgtcc aagcagcacc
    56521 tgtagaaaac gcagaggtcg tttccttatt agggatattt acgtatgcta ttgccgcatt
    56581 cgtcattatt tttttggcaa tcgatgcatt gatccaaatg tataagaaga ggaaaaagta
    56641 gaaaaaggtt gtgacagaaa gaatcagctt caagaaataa gaaggggttt tacataaatt
    56701 gtttctcaaa tttttgtgaa aattcggctt atttttcgaa gaggctactt ctgaaacacc
    56761 gagaataaaa aatgtgaaaa agctgttctg cacccagtaa taagaacaat caaaaaggga
    56821 acatggaaca catttatcct gtttccctgt tccctttttg atgtattcga gcataaataa
    56881 aataccttcg ttacctacta tcttctgtac ttggtaaacc ttagtcaatt gcctttttta
    56941 acgcttgacg aaaagacata tgatctttct ccgtaaaggg ttttggaccc tttgtcctta
    57001 tgccagcttt tcttaacttt ccactatgat aacgttgttc tagaagcgtg ccgatattcg
    57061 cttcgtcaaa ttcttttcca gagtgatgga acactcgcac acccttcggc aatacaagtg
    57121 aagctaaacc ataatcttgc gtgatcaaaa agtcattctt cttaaccata ccgacgattt
    57181 tataatccgc actatcagct ccgcggtcta cgtagacgat ttggacatga ttagcgtatt
    57241 ctttggtcgt ataatggtcc acactggtca cgataaccac gtcaagacca taggattctc
    57301 ctaattttat gacatcttct tttactggag aaccatctcc atcaatgaat agcctcattc
    57361 tttttcgcct tcttccataa atgataaaca gttactaatc cgctcagtaa aagaccaaca
    57421 ttgatccatt ttgccaagat aagcagttgt acactttcta tcggcactga ttcatggttc
    57481 agcgtgatca ataatagatg gattggctct cccatttctt ttgctaaaac aaacaaaagc
    57541 gtcatcaata aaaactggtg gatcacactc cctttatttt gagtagtata cttgacaacg
    57601 atccggcgat ccattggaat agctgtatca gtttgataat attccttatt tagccagctg
    57661 cctgttacta cgtgccagaa actaagcaga aagacaccta tcgcaaaacc atttggaatc
    57721 aagaggacca ataaagaaga actcagtcca agcaattgca tagccaatgg agaccatgtt
    57781 aaacgtttca aaatccatgg tccgactatt tttgctgcaa tcaagcctgc tgcataaggc
    57841 aaatacataa aaatcgaaag tttttctaat ccgtacagta tgccaacata aaaactgcta
    57901 aataaaaaaa ggaaattccc tagcattcca tttagaaaag taaacagatt tagccagctt
    57961 ggcaacttcc attccttctt ctggtgtcct aaaacaaaca cgaacaagat gaacagcagc
    58021 aagaaaccat aaatagcaag atcaaaaagc ctttcatcga ataacaatcg ggcatttctc
    58081 aaaagaaaca ataagccaaa gaatatcaaa aataaaacaa gctctcgcgt agctaccaga
    58141 tttcttttga tgtctttgaa atccagttcg taacgcggat aatgactaac cgtatggtag
    58201 gccatgacgt aaaaaagtgt ataaatagta aagcttgctg caatctgagt cggtgcaggc
    58261 aactcaatcg cttgatacag gagaaatagc cagatagctg caaataagta ctttttccca
    58321 tccatattga taaaaccttg cgtcttttcg tgaaaattga ccgtggtatt agcaggtaca
    58381 agccatgctg cactcagccc tagcaaaaca cttgaaaaat aaaataaagg aaaataaaat
    58441 acgcctaata ttccaagcaa tgaaccagca ccaccaatca gcagagcgat catcagcaac
    58501 gtgtaactat ctagaccaaa tcgtagactg ttgactaaaa atagtccagt catccgaaag
    58561 gcataaaaca gcgtaaatgg caaaatcgtc agccaattat gcgagtctgt tagtcccaaa
    58621 aataaaagat aaggaataaa aataaccgtg ttacttaaga taaagggcgt tgtttgtgta
    58681 aaattacgcc caaatcgttt gagtttttcg aacatgacaa tggctcctta aacagtctta
    58741 taactaatct gctcttcgat tttagcatta aaggggaaat aaaccaattt tacagaatat
    58801 attcttgtaa aaaaaaatac agaaagaagc ataagcttct ttctgcattt ttacggtact
    58861 tgattactca agcagacaag cccttctcca caaaagtctt gcccgacacc cctgcatccg
    58921 gcaggtgata aagatagaaa tcaattatcc tgtgcctcca aaatcaatta ggagctctgt
    58981 aaaccagagt ccttctcgac ccgtcgcata aatttacttt aacacagttc ctatgtttat
    59041 gcgagagctt ttttcatttt ataatcgacc atttgaaatc cttgaccgat atctgaaata
    59101 agttcttctg taatctcaaa tccttcatgc ttataaacct cgattgcttg atgattcccc
    59161 tgattcactc gcaaaaagat ctctctttcc tttgtttgcg cagcaagttc ttcataccag
    59221 cgaaaaactg aagaagtcaa tccttgacca cgtactttat ttagcaaata taatttactt
    59281 aaataaattt ttccatttat ctcttcataa gcagtgtaac cgacagtctt ttcttcgctt
    59341 tttaataaaa agtaggacac accttctgtc agttcttttt gaatctgttt ttttgactga
    59401 taagttttca gcatgtaatc cacttgtttt tgaccaatga tcggcacaaa aacttccggc
    59461 catacttctt gtatgactgc catcagctca tccagttcat tttctgttac ttttattttt
    59521 tccatacacc cacgtccttt tttacattat aaatgacctt gcgcttgatg gaataaaaag
    59581 agcatgatca tgctatacac acccaccgac cacggtttca agaaaatcac aatctgcata
    59641 aattccctaa aagataattt cgtcaaacca gctactagac agatcaagtc gtctggcgca
    59701 aagggaagca gcaaggtaac aactagtact tttttaatat ctttcgtttt tcttgtcagc
    59761 aattcatcaa atttctgata cttttttggc gataaaataa gctggacaaa ggctacgcca
    59821 tagtaacgga caagcaaaaa tccgattgct tccccaatca ctaggccaat acaactatat
    59881 aaaagcccaa atccattgcc aaacatcagc atgcccgcta ctgaggagat ccctcccggc
    59941 aaaataggaa tgatcacttg gatcacttgc aacagaacaa atatcatcac tgcatacttg
    60001 ccaaattgct ggataaacgc ttgcaaagac gtgctcgatc gaaaaatccc atgatgataa
    60061 gcataaagaa tcaacacaaa gaaaaaaaac agaccaataa tgggcatgat ctgaagacat
    60121 ttttgtaaaa ctgatccttt tttagacatc tatattcccc acatcttttt tcatttatta
    60181 aaactgcctc ctt
//
LOCUS       DACDXO010000009        57129 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E23605_2017
            SAMN12639989-rid9881463.denovo.009, whole genome shotgun sequence.
ACCESSION   DACDXO010000009 DACDXO010000000
VERSION     DACDXO010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639989
            Sequence Read Archive: SRR10023389
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 57129)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 57129)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741914.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:42:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,972
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 33 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 17 of 73
            Pseudo Genes (multiple problems)  :: 10 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57129
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639989-rid9881463.denovo.009"
                     /isolate="E23605_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..257)
                     /locus_tag="IWM35_000687"
     CDS             complement(<1..257)
                     /locus_tag="IWM35_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9918562.1"
                     /translation="MERQESIREVQEVPTEQAKETQEGRSFSYGKNFSFLRPSIQISV
                     FVLWRIHNNRYSVGSQLFYQEICHALSKKYSQNAYKEALAF"
     gene            complement(273..923)
                     /locus_tag="IWM35_000688"
     CDS             complement(273..923)
                     /locus_tag="IWM35_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9918563.1"
                     /translation="MKKRFIYVCVGGLFLFGVGSTFFLIHEKLQLTAKEEKLTVEINK
                     FQEEKKGQEDLVTSLKEQLQQVESEVTTLKKANEELKNNDSTEKGNIQTFQKTVTEAF
                     EVLFNYEPDSFQERKNNCAPYLSDKLMKQYFKEGVSYGDSNGVSSELEKCSVYLKSTQ
                     KATIQGLVVVSYRNKIGDSSWNNAMNIFDVTFDPASKKITFMQNLGSSFNQQLLEE"
     gene            complement(934..2106)
                     /locus_tag="IWM35_000689"
     CDS             complement(934..2106)
                     /locus_tag="IWM35_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HAP9918564.1"
                     /translation="MKKKLLLWCGFGCLPTLLLCMFGFLFMILLISSGSEEEEIDCSP
                     TEIVASTISLTNQGMETNGLQIAKEVQKHIPNTTLEGLAGMLGNFQQESSMSPTAIER
                     PNDPLSGHGIAQWTAGRTTALKNFAKERNKEWSDLGVQVEFLIHELNTTEKASQVALQ
                     AKSVEEATEQWQVKFERAGIPMMGNRITFANQWYAKLGKNDSDPVASITLENGANAGS
                     KDLTDYCLETTGTSSSSDSNVLNVAKSLIGYFHYSQPQRAQFGSVENPDKNGYADCSS
                     FVWLVLTKAGYKTPANVGWFTGSMTSDARGSKQYLQEIPESEAKAGDIIIVNLGSGVG
                     NDGHTAVLAEDWHGLSTKVIEQGGMNTNGVGEGQVNLSFGYLLNGGDICFARPIKK"
     gene            complement(2125..2997)
                     /locus_tag="IWM35_000690"
     CDS             complement(2125..2997)
                     /locus_tag="IWM35_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP9918565.1"
                     /translation="MKKMILGSLFSATVLLGMVGTSAYADTTSPGDPSVPPTEIIPPT
                     EETPEVPEEPVLPPTDGGEETPEIPEIPETPETPEIPPTEENPDTGGGTETPEVPENP
                     DTGGGEGTPEVPPVEENPGNGGNTETPEKPSNPGGNEGNTTPPTTPPTNNGGTNTTPP
                     VQDVVVKPDGSIGSQQTGGQNVQIETNNASELTHIPTPSTPIETASGEKIVSVVDGVA
                     YKESGNGLTPISDKVEQLPSGNIAVKGSDGKMKVLPKTGEEVSLVATALGGLLASGSG
                     FAFWRKRKATQKMN"
     gene            complement(3019..3939)
                     /locus_tag="IWM35_000691"
     CDS             complement(3019..3939)
                     /locus_tag="IWM35_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9918566.1"
                     /translation="MMMIRFTEVDNMSLLTDKKIALDKAFIQLKRENEELLARAEIGW
                     CTLEIYTENHEIPVFEGSYDFPVEEFHLISLIEQSLTENGQLSEEAQTFIQTLNSELP
                     KFYQVKKDQKNERRPTKIKKEKKTPLSVETAFTQSNGSLQKKKMSFQWLLFGFIVVVS
                     CGLFIMQLSTQREIEQLTKKEHQLQERIAFAEELATKQPQIDTFSRYFITNYYSGEMA
                     EERYRKKLQSFLTEKVAKQISPQKEKVKSILSWEMKKEGKHWQIAYVITLETEKKTQE
                     TKKVSFQLEEQEKNYLVSTLPELDDFEINQ"
     gene            complement(3939..4208)
                     /locus_tag="IWM35_000692"
     CDS             complement(3939..4208)
                     /locus_tag="IWM35_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9918567.1"
                     /translation="MIIVTGIVLLGIGVLFGYHFAYFPKIGEWQQRQAIQKYFQVKGN
                     EYTYYAEDEDSYIVSLNNQEYRVKFSLNNPIQVVYAVEVVEEVFD"
     gene            complement(4205..4585)
                     /locus_tag="IWM35_000693"
     CDS             complement(4205..4585)
                     /locus_tag="IWM35_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HAP9918568.1"
                     /translation="MITNYKKFWQNSFNYKGKATRKEYWLVALMNALIGFGLGLAIGL
                     AGALNASETIIGCLIGVLIVYCFVVLVPSLSLSVRRLRDAGFHWAFIFFYFVPTIGSL
                     VLLVLYCLPTKESIEDKQVQEETR"
     gene            complement(5052..6515)
                     /locus_tag="IWM35_000694"
     CDS             complement(5052..6515)
                     /locus_tag="IWM35_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HAP9918569.1"
                     /translation="MKKTSKCLSALLLVGQVFGYTPAVLANEQGNNQLTNSQSATITE
                     ETTTNSTGEENQPETPTEDTIDTPDNPEMNAPPTGSEEQPETPNQPDKPEDNQQPEEE
                     PSEEPNEQPSDESSQTPQKPSTETEAEPPTKTERPQKAPEQSVTVPMNDVPQAQSIGS
                     EETKNYHQAPSAPIQELVPSSTQDNEGTIHFEKDASVETFIRKIGESARKIGQENDLY
                     ASVMIAQAILESASGQSQLAQAPNYNLFGIKGTHNGKGVSFATQEDLGNGTLYTTQAT
                     FRQYESYEESLKDYAKLLKEGLVGNTSFYDGVWKSKAKTYQEATKFLTGRYATDTSYD
                     KKLNGLIETYDLIKYDKEVAGPQLSKEGYMVPLKNYSISSSFGWRGEEFHRGLDMAAN
                     QGEPIYASKAGTVIEASYHTLSGNYVTIEHEDGSVALYAHQQEYCVNVGQTVEQGQII
                     GYVGSTGNSTGPHLHLELALDGSLTKDKLINPETVLF"
     gene            complement(6579..9596)
                     /locus_tag="IWM35_000695"
     CDS             complement(6579..9596)
                     /locus_tag="IWM35_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP9918570.1"
                     /translation="MFKRMATWLAIFVTFLNPMLGSVTAFAETINESNSTEEVMKTEE
                     SKSSEEAISTETTSETTEETTTQDTTEDVSPPNVMTQEVPEGYEKYFITIEDIQKATS
                     FSEQERLAQESEEWQAILEKNAQTRATNLTLTENGRKDVNMTRITPEGTMYTHDYMVR
                     WQIDGEDVFCIQEGAITSAGIQYDVTPLENLITTSAIRKKISLIGYFGYYSSGSMDNY
                     VLAQTYIWEVLGGSFVNYGTIGQSNYEAFKQTVDRKINNLLEDPSFDSQTVTVKVGEE
                     VEVKDTKGVFSQWDGSVVENTANVQVRKSGNSLFIKANQNSNDGRISVSSLDPNSRGV
                     GSSFAYVDPVAQNLGKLYVPDPNRAGLTIKVLKNGDFKLTKLDAVTGKKVPNVTYEIK
                     RHDTGETKQYTTDAKGEIALKEIPAFYEKDGKYVQNKLDVKEVKAPAGYILDKQTHTI
                     TIEPNKEVTLTAKNDLQQFIPEGKKVKEVYNAEESWNQGKPVYDTVPAENIEFDIIAK
                     TDITAPDKETVLVKKGEVLDSVKTDKDGHWKANKKFYNGDQNKYQLIEKNVPDNYRQP
                     SEEQTTFSVAYGDNTTPVLTFELDVIDNLLKTGEVDFNKVDSINHLQLPGAKFLIEGM
                     TAHNKNVSFVYETKEEPEKLKLPDGTYKFTEIVFPEHYGQASGQTETILVTVKDGEVQ
                     PITVKNEEIVVPEEDQPEISTLFVTTTGGKDFNPKKDQELVDKVSQVFPKSAVGTEKY
                     WVTQFHKITADGKVTVLDTVKETRKVTDEKETFDVTFNYKADMVSDGDTIVATHIVYN
                     DKEEEEEFARHFDLTNKDQTVTAKEPKIETRFITVNGDQTFDPDKDQELIDHVSQEFP
                     ESTVGETKYWVTQFHKIDKNGKDTVVGTVETARKVSKASEEFKVSFDYKAGTLKDGEK
                     LVATHIVYKDKDHKEEEARHFDLKNEKQTLTAKGIKKETPTTPVKQTPTSTPKQSLPK
                     TGETVNDVLIALGLFLFVGVGAVLVRRKMKQSN"
     gene            complement(9747..10586)
                     /locus_tag="IWM35_000696"
     CDS             complement(9747..10586)
                     /locus_tag="IWM35_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP9918571.1"
                     /translation="MKMLKVLALGIVVGGVSLAVGTNAEAAENKVWTPRSAEQIKAEI
                     KGNEYTIVWGDTLSAISQATNITVQKLADMNKITNVDLIYAGNKLVFNGNVASVQNSA
                     GETVAQTVIQEKDKVDPNQPVGQGTTSNTQTTPNTTTPTTPAGDQTGTTGGTTTPSTP
                     ENNGGTTQTPTQPGNGGNTGGETTPSNPSTGGGTTTPEEPSNPGGGDQGGNGGTTTPE
                     EPTIVVGEIGNSGKVFNTVDEAYDWANAEVSDPNSQWYNKGFMVDVIIYSDGSQKHTV
                     DFY"
     gene            10731..10976
                     /locus_tag="IWM35_000697"
     CDS             10731..10976
                     /locus_tag="IWM35_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014862476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9918572.1"
                     /translation="MQLNNLNEIHDWAQNNLLNRKQAAKITGQNYDAFSSSLRRNNIK
                     PFLEIGDEPGPSIIRLYLREDIEKYAKQLEEKRQKRQ"
     gene            11039..11461
                     /locus_tag="IWM35_000698"
     CDS             11039..11461
                     /locus_tag="IWM35_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014862359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9918573.1"
                     /translation="MNKKYIVSVGSIVLIVLAIIAFSVSNKTETQPQNTVQSTTTSQS
                     SDHSVYPSLNSLKETHGQDLIDLGSSQITTVLFEKKNIAFITPKEGTDLEFSIKVTNT
                     KENLFSIPDIQYTPNKLKIGDTFGLAKVNEQYFAYSLK"
     gene            complement(11555..11872)
                     /locus_tag="IWM35_000699"
     CDS             complement(11555..11872)
                     /locus_tag="IWM35_000699"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9918574.1"
                     /translation="MPVKGTSNFDFEIFYVDDEICGVFNKQKYTKEQAIEAWKNELFD
                     PEEEISFVVDESFVGWYHGNAEEEEVHYAGWWLEEGEPREQSVPVWVISENPLEEIEK
                     LEK"
     gene            complement(11899..12105)
                     /locus_tag="IWM35_000700"
     CDS             complement(11899..12105)
                     /locus_tag="IWM35_000700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9918575.1"
                     /translation="MNWLKNEAELYVFHEEMQEKHLVPNVRNYLFWLEEKNDAQVFGK
                     SAQSPKIKLQLTSKQWNEHEWYIY"
     gene            complement(12086..12268)
                     /locus_tag="IWM35_000701"
     CDS             complement(12086..12268)
                     /locus_tag="IWM35_000701"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9918576.1"
                     /translation="MTKCERVSVTISFIALLLSVISLLNTAIFIGQVEKQKEISLSEW
                     RYEEVNEGMNNELVEK"
     gene            complement(12282..12632)
                     /locus_tag="IWM35_000702"
     CDS             complement(12282..12632)
                     /locus_tag="IWM35_000702"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9918577.1"
                     /translation="MKKSYKVFENNEIKEQEAIILSAQTPEEALEQALPFLVESQNIV
                     VEELPEFDSKIVFSFNCKKESEISVSPEFYCRSDISVEGLVMCASAFIEIFANHLQIS
                     VKELLDLLQEITNE"
     gene            complement(12658..12960)
                     /locus_tag="IWM35_000703"
     CDS             complement(12658..12960)
                     /locus_tag="IWM35_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9918578.1"
                     /translation="MLSKQEKKYIRQKMYAMIGNRAITELSSEELLQVQQLAQIIGSN
                     SIFDSNPLPEMTLETLTAKRYKELRAIGYRMIDIRRALRISDTKFREWRTSNGLAN"
     gene            complement(12963..13196)
                     /locus_tag="IWM35_000704"
     CDS             complement(12963..13196)
                     /locus_tag="IWM35_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9918579.1"
                     /translation="MTISVGNKVKYIGTAIPRYTNKLVVVKNILVNGLILEYPKEDRR
                     KVVVEGNGTWNMNSFICGFTEVEVTNDNENRSG"
     gene            complement(14182..14504)
                     /locus_tag="IWM35_000705"
                     /pseudo
     CDS             complement(14182..14504)
                     /locus_tag="IWM35_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347542.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(14627..15742)
                     /locus_tag="IWM35_000706"
     CDS             complement(14627..15742)
                     /locus_tag="IWM35_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9918580.1"
                     /translation="MATKRFTATRKYNELYYQFPKVFITSEKYRSLSDKAKIAYMVFR
                     SRLDLAIQRNQVDSQGFVYFEFTEQELANALNCCRRSVNTIKKELKEYELLEIEDMGF
                     DQQTGKRNKARLYLGELDTTENDVYSLGPVDSTSVDSAQGKEEEESVDNLAIFDDFDE
                     KNPCENSAQLFNNSSNNLDTENLDTKIDTTSPVEMADDWQKEFIAHYSESTFVPKDVL
                     TWIVRSSAEWREAKEQLNMIYDSKRIIEKRQRKYNQTHGLSDGSEYRILGETFAEDLS
                     KTYQRCIQNEKIARHEGKAIQNRKGFYFRALLNFWECCVRELNLDPMATKLSDQAEYS
                     LLQAKRSCSYEDLETQIYGLSQLPKISMHNWLEELDT"
     gene            complement(15748..16146)
                     /locus_tag="IWM35_000707"
     CDS             complement(15748..16146)
                     /locus_tag="IWM35_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9918581.1"
                     /translation="MVKNKFRATDVLTQATFHMPKLFFIKSSKYYAMTAQAKYAYSLL
                     NEYLEESLIEKRIDEKKHVYISYTNQELAILLNVSEEEIEEIKNELEDYDLLTIEFGR
                     IYLKELIATMEDVRVYQHQNSFLIYGQDEV"
     gene            complement(17175..17561)
                     /locus_tag="IWM35_000708"
     CDS             complement(17175..17561)
                     /locus_tag="IWM35_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HAP9918582.1"
                     /translation="MNKRKTEFILILISGITGILCSLLALMGGMFLSSIDSTTLNTTE
                     LTSKDIDMLVNLSGNVVLFGGITLVFSIMIFIIGFLVRKNQKTVVYGISLLILSIIPF
                     FLLTLLWVIPGVLGIIAGLMLLIRKS"
     gene            complement(17558..18079)
                     /locus_tag="IWM35_000709"
     CDS             complement(17558..18079)
                     /locus_tag="IWM35_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HAP9918583.1"
                     /translation="MNKKSGIETLPNYVFAGFWLRFFAFLIDLICISIITHATIGLFY
                     KIMGLESDKSIITFYGIASLAIYLTYFVLLTKLNSGQTIGKMIFGIRVVSFEEKELSW
                     QTIFIRECICRFILKNFVFQIGYLVAAFTPKKQHIGDYFSNTSVVVIRSLEAINYGQK
                     ISNSKTLNLERIK"
     gene            complement(18081..19115)
                     /gene="sppA"
                     /locus_tag="IWM35_000710"
     CDS             complement(18081..19115)
                     /gene="sppA"
                     /locus_tag="IWM35_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HAP9918584.1"
                     /translation="MNKRRWIAVGVACGLLLLSIIVALIPGKDKEEASNTTLTGINKI
                     FYGSNEITEETLEEGASNKKIVKLSVNGVIADTGESNLFSREQYNHQNFLTQLKKIQE
                     DKAVKGVLLEVNSPGGGIYESAEIAKEMAKIKKLDIPIYTAFKNTAASGGYYISAGSD
                     KIFATEETTTGSIGVIISGLNYSGLLEKLGVTDATYKSGALKDMMSPQHKPSEEENKV
                     IQEFVMSAYDRFVNVVAKGRNMDTNAVKELADGRIYDGNQAVENGLVDQIGYSEDALD
                     SLKKEKKLTDATVIEYKNDTTGFASSWLGNKIAEWQGLKATTSDRVISVFEKLGFSDT
                     PKPMYYYGGL"
     gene            complement(19148..20563)
                     /locus_tag="IWM35_000711"
     CDS             complement(19148..20563)
                     /locus_tag="IWM35_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HAP9918585.1"
                     /translation="MVSIKNYGKSRRGKQFMQNNNWFEHSSIYTKQHHSFYRWVLYPV
                     ILFLCIIGLFLTFAKKEVVIRTTAQLNPEKIEKLQVPLEAKIMENFLKENQFVHKDDV
                     LVRLDCSLIENEKAQIEQENQRITQQIKMAQLFIESISKGKNLFSTDDSFGYSNQLKS
                     MLSEKESLRYALKQSELNDQKQLEVYEKTKRQLEKQIESSDSKLQEWQQVQVAWSNNQ
                     SLKDFSKEMMANYENWQEQLNNVSDDQKNQVKLTISASINEQIEQLKKEVEQYQSEKA
                     KLVKPTTSENDRISQTEKGKQELEQAITTVKQTVTELNEKQEKNQSVIKSLDDQLSKG
                     ILKAPVSGTVHLNEETKGQAELAKGTVLAEIYPIHEKSKMKFTALLPANESTYIKEGM
                     KVHFKLDQKGGAPITIDGTLDEISENSTATEKGVFYAVKGTLKTTQSFPFRYGLTGEL
                     SLIVGEKTYWQQIVDVFLNKK"
     gene            complement(20532..22685)
                     /locus_tag="IWM35_000712"
     CDS             complement(20532..22685)
                     /locus_tag="IWM35_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide cleavage/export ABC transporter"
                     /protein_id="HAP9918586.1"
                     /translation="MIKSFPFVQQQDEKDCGVACLSMILKYFKTDIPIHKLRDLSGTD
                     LEGTSAFGLKKTFEKLSFDCMAIQADSEIWKERDLILPLIAHILVDNSYMHYVVIYKT
                     KGNVLWIADPANGKNRKTIEAFQKEWTGILLLPMPRETYIPIKEKVAGLSSFLPIIWK
                     QKGLVFHIVLASIFITFFGIGSSYYFQGILDFFIPNQARSTLNIISIGLIAVYLFRVL
                     FEYSRSYLLIILGQRMSMAIMLQYFKHVLTLPMNFFATRKSGEIISRFLDASKIIDAL
                     ASATLSIFLDIGMVLLVGVTLAIQNSTLFLITLCSLPFYVVAIFAFVKSYEKSNREEM
                     AAGAAVNSSIIESLKGIETIKAYSGEEKVYNRIDHEFVKLMKKSFRTVTLDNIQQGIK
                     HLIQLISSALILWLGSYFVMDNRISLGQLITYNALLVFLTDPLQNIINLQVKMQTAQV
                     ANNRLNEIFAIDSEKSNQSFEYMLSEQLFQQDITVKNVSFSYSMKAPTLNEISCTIPS
                     HSKTAIVGVSGSGKSTLAKLFVRFYEASEGSIHYGNINHLDIPFQILRDRVTYLPQES
                     FFFSGTIIENLIFGLDSPPSFEQIIEVCEIVQLNEFINQQPLRYETLLEEGGNNLSGG
                     QRQRLALARALLKNPDILILDEATSGLDTLLEHAIMNYLLNLKEKTIIFIAHHLPIAK
                     ACDQILVLHEGHLVEKGTHDELRFADGIYQKLWEI"
     gene            22943..23179
                     /locus_tag="IWM35_000713"
     CDS             22943..23179
                     /locus_tag="IWM35_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HAP9918587.1"
                     /translation="MFKHAKCKGTKYCGNAQTVGGGNNAWGKLGQVVGGLTTGAVGGA
                     GLGTAICGPACGVVGGLYGAVAGGAAAGWDARKK"
     gene            23198..23413
                     /locus_tag="IWM35_000714"
     CDS             23198..23413
                     /locus_tag="IWM35_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HAP9918588.1"
                     /translation="MHIKNTKTTFILSSEELKNIQGGSAVGVLGTTFSGATAGVKLCS
                     AGGPYAIAACGVGGALLGAGFSMWTGA"
     gene            complement(24116..24868)
                     /locus_tag="IWM35_000715"
     CDS             complement(24116..24868)
                     /locus_tag="IWM35_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9918589.1"
                     /translation="MSYPIILCEDQIIQLNQLERIIDNFILFHDKVFKIVLKTQSPLE
                     VKKYLKQFRPKQGIYFLDIDLNHEVNGIELAEVIRKYDVQAKIIFTTTHDEMLPVTIK
                     RRVETLGFVTKDQTLDEYRNEIVELLLLAQERIDATKQVTNQAFVFSIGSQTFTFDIN
                     DIYFVEASKLPHRLSLCTKDGQYEFYGRISELEKKYPMLTRISRACLVNIFNVKEIDF
                     KKRSLYFDSELARNFTLGKAQKIKEKLKESTV"
     gene            complement(24883..26130)
                     /locus_tag="IWM35_000716"
     CDS             complement(24883..26130)
                     /locus_tag="IWM35_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAP9918590.1"
                     /translation="MFVGNFFFFLLFYMTNTDLNKRKYLFVCLLIPLTFFVELFTDVS
                     AAIPIIGSYFILKEKKKTNIVLLNKLLLCMLIDDFRSIITSMVMTYMFSFEGIKGYGY
                     VVFQIMLDSLFLLMFIWLYRKLAINRIVEQSSSKLTAIFMIYLLTIGLFVSYVAHQYQ
                     VFDHFVFGVLIFLIIQMFVVLFLFIRITIRQREQYEQQLKRKELVYLKKYTDSLEKDQ
                     EKFARFRHDYKNLLLSLKEIANQNHDSILEKEIKELEIYSNSYLTNKFEYRYLKNIKN
                     EYLKSLLIAKLYQANNNKICCKFECPSTIIEVPVPIFDCIRVLGIILDNAIEASIESQ
                     DRELSLAIYRDDKQIEISISNSCREILLSIDTLMKKGITTKKGHQGLGLSSIEEINKK
                     NPNMFVNYQNDPYQFTTQVILLF"
     gene            complement(26206..26352)
                     /locus_tag="IWM35_000717"
     CDS             complement(26206..26352)
                     /locus_tag="IWM35_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EntF family bacteriocin induction factor"
                     /protein_id="HAP9918591.1"
                     /translation="MEEKNRLNAKQCSDQELKKIKGGAGTKPQGKPASNLVECVFSLF
                     KKCN"
     gene            complement(26456..26767)
                     /locus_tag="IWM35_000718"
     CDS             complement(26456..26767)
                     /locus_tag="IWM35_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HAP9918592.1"
                     /translation="MKKNAKQIVHELYNDISISKDPKYSDILEVLQKVYLKLEKQKYE
                     LDPGPLINRLVNYLYFTAYTNKIRFTEYQEELIRNLSEIGRTAGINGLYRADYGDKSQ
                     F"
     gene            complement(26769..26966)
                     /locus_tag="IWM35_000719"
     CDS             complement(26769..26966)
                     /locus_tag="IWM35_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterocin"
                     /protein_id="HAP9918593.1"
                     /translation="MKHLKILSIKETQLIYGGTTHSGKYYGNGVYCTKNKCTVDWAKA
                     TTCIAGMSIGGFLGGAIPGKC"
     gene            complement(27360..29087)
                     /locus_tag="IWM35_000720"
     CDS             complement(27360..29087)
                     /locus_tag="IWM35_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP9918594.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            complement(29080..30273)
                     /locus_tag="IWM35_000721"
     CDS             complement(29080..30273)
                     /locus_tag="IWM35_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HAP9918595.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            complement(30460..31104)
                     /locus_tag="IWM35_000722"
     CDS             complement(30460..31104)
                     /locus_tag="IWM35_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP9918596.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            complement(31198..31332)
                     /locus_tag="IWM35_000723"
     CDS             complement(31198..31332)
                     /locus_tag="IWM35_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HAP9918597.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            complement(31334..33466)
                     /gene="feoB"
                     /locus_tag="IWM35_000724"
     CDS             complement(31334..33466)
                     /gene="feoB"
                     /locus_tag="IWM35_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HAP9918598.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            complement(33463..33936)
                     /locus_tag="IWM35_000725"
     CDS             complement(33463..33936)
                     /locus_tag="IWM35_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HAP9918599.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            34337..34561
                     /gene="nrdH"
                     /locus_tag="IWM35_000726"
     CDS             34337..34561
                     /gene="nrdH"
                     /locus_tag="IWM35_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HAP9918600.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            34720..36879
                     /gene="nrdE"
                     /locus_tag="IWM35_000727"
     CDS             34720..36879
                     /gene="nrdE"
                     /locus_tag="IWM35_000727"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HAP9918601.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFLSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYAMAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            36929..37894
                     /gene="nrdF"
                     /locus_tag="IWM35_000728"
     CDS             36929..37894
                     /gene="nrdF"
                     /locus_tag="IWM35_000728"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HAP9918602.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(37983..39122)
                     /locus_tag="IWM35_000729"
     CDS             complement(37983..39122)
                     /locus_tag="IWM35_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP9918603.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAV
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(39431..40144)
                     /locus_tag="IWM35_000730"
     CDS             complement(39431..40144)
                     /locus_tag="IWM35_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HAP9918604.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(40398..41099)
                     /gene="nagB"
                     /locus_tag="IWM35_000731"
     CDS             complement(40398..41099)
                     /gene="nagB"
                     /locus_tag="IWM35_000731"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HAP9918605.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            41333..42865
                     /locus_tag="IWM35_000732"
     CDS             41333..42865
                     /locus_tag="IWM35_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAP9918606.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(43056..44258)
                     /locus_tag="IWM35_000733"
     CDS             complement(43056..44258)
                     /locus_tag="IWM35_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP9918607.1"
                     /translation="MKSNEKKPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSAVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMAALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTVFMFMYFIGGAAGSLLGTTMWQQYGWLGVTVSGLVFQGCAFFFQTVVFKG
                     KRNLENKK"
     gene            complement(44340..45029)
                     /locus_tag="IWM35_000734"
     CDS             complement(44340..45029)
                     /locus_tag="IWM35_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAP9918608.1"
                     /translation="MKKKIIRLILISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(45135..45503)
                     /gene="rplL"
                     /locus_tag="IWM35_000735"
     CDS             complement(45135..45503)
                     /gene="rplL"
                     /locus_tag="IWM35_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HAP9918609.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(45556..46059)
                     /locus_tag="IWM35_000736"
     CDS             complement(45556..46059)
                     /locus_tag="IWM35_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HAP9918610.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     gene            complement(46469..47158)
                     /gene="rplA"
                     /locus_tag="IWM35_000737"
     CDS             complement(46469..47158)
                     /gene="rplA"
                     /locus_tag="IWM35_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HAP9918611.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(47262..47684)
                     /gene="rplK"
                     /locus_tag="IWM35_000738"
     CDS             complement(47262..47684)
                     /gene="rplK"
                     /locus_tag="IWM35_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HAP9918612.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            48027..48695
                     /gene="sdaAB"
                     /locus_tag="IWM35_000739"
     CDS             48027..48695
                     /gene="sdaAB"
                     /locus_tag="IWM35_000739"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit beta"
                     /protein_id="HAP9918613.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            48708..49580
                     /gene="sdaAA"
                     /locus_tag="IWM35_000740"
     CDS             48708..49580
                     /gene="sdaAA"
                     /locus_tag="IWM35_000740"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HAP9918614.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(49671..50690)
                     /locus_tag="IWM35_000741"
     CDS             complement(49671..50690)
                     /locus_tag="IWM35_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
                     /protein_id="HAP9918615.1"
                     /translation="MEAGAFGYIRGGSEDEWTMKENTTSFNTKKIMPRILRGIDSADL
                     HTSVFGIELDTPIIQAPSAAQGLAHEKGEADTAKGVAAAGSIFSISTYANTTIKDAAD
                     AAPGAPQFFQLYMSKDDRFNEFILNKAVEAGAKAIILTADSTLGGYREEDVINQFQFP
                     LPMPNLAAYSEQSASGNGEGKGIAEIYAAAKQGLTPDDIKTIKEITHLPVIVKGIQSP
                     EDAVIAISAGADGIWVSNHGGRQLDGGPASFEVLPKIAEVVNKRVPVIFDSGVRRGEH
                     VFKALASGADLVAIGRPVIYGLNLGGAEGVTSVFEHLNKELSITMQLAGTKTIDEVKN
                     TKLMD"
     gene            complement(50863..51750)
                     /locus_tag="IWM35_000742"
     CDS             complement(50863..51750)
                     /locus_tag="IWM35_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAP9918616.1"
                     /translation="MTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEVSDKEKT
                     AESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDGQIQATG
                     DLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSMGGVSAL
                     RYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQDYQQMI
                     GNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTGENASHS
                     MLHENQEVDQLVSRFIWKE"
     gene            complement(51827..52372)
                     /gene="nusG"
                     /locus_tag="IWM35_000743"
     CDS             complement(51827..52372)
                     /gene="nusG"
                     /locus_tag="IWM35_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HAP9918617.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(52473..52643)
                     /gene="secE"
                     /locus_tag="IWM35_000744"
     CDS             complement(52473..52643)
                     /gene="secE"
                     /locus_tag="IWM35_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HAP9918618.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(52667..52819)
                     /gene="rpmG"
                     /locus_tag="IWM35_000745"
     CDS             complement(52667..52819)
                     /gene="rpmG"
                     /locus_tag="IWM35_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAP9918619.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            52981..53625
                     /locus_tag="IWM35_000746"
     CDS             52981..53625
                     /locus_tag="IWM35_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAP9918620.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(53681..54184)
                     /locus_tag="IWM35_000747"
     CDS             complement(53681..54184)
                     /locus_tag="IWM35_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HAP9918621.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(54197..54243)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            54781..55746
                     /locus_tag="IWM35_000748"
     CDS             54781..55746
                     /locus_tag="IWM35_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HAP9918622.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGDDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(55845..57017)
                     /locus_tag="IWM35_000749"
     CDS             complement(55845..57017)
                     /locus_tag="IWM35_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9918623.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMPTIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(57004..>57129)
                     /locus_tag="IWM35_000750"
     CDS             complement(57004..>57129)
                     /locus_tag="IWM35_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9918624.1"
                     /translation="KLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
BASE COUNT        17012 a        11484 c         9386 g        19247 t
ORIGIN      
        1 aaaaaggcaa gtgcttcttt gtaggcattt tgactgtatt tttttgataa agcatgacaa
       61 atttcttgat aaaatagttg actgcctacc gaataccgat tattgtgtat ccgccacaaa
      121 acgaacacac tgatttgaat cgacgggcgt aaaaaagaaa aattctttcc ataagaaaaa
      181 gaacgtcctt cttgggtttc ttttgcttgt tctgttggta cttcttgtac ctctcttatt
      241 gattcttgtc tctccataac cttctccttt tcttattctt ctaaaagctg ttgattaaag
      301 ctacttccca aattttgcat aaacgtgatt ttctttgacg ctggatcaaa ggttacgtca
      361 aaaatattca tggcattatt ccaggaacta tcaccaattt tattgcgata cgacaccaca
      421 acgagccctt ggatagtcgc tttttgtgtg ctttttaaat aaacagaaca tttctctaat
      481 tcagaagaca ccccgtttga atcaccatag gaaacgcctt ctttgaaata ttgcttcatc
      541 agtttgtcag aaagatatgg tgcacaattg ttttttcgtt cttgaaaaga atcaggctca
      601 taattaaaca gaacctcgaa cgcttctgtc actgtttttt gaaaggtttg aatgtttcct
      661 ttttctgttg aatcattgtt tttcaattct tcatttgctt tttttaacgt cgtgacctcg
      721 ctttctactt gttgaagttg ttctttgagt gacgttacaa ggtcttcctg tcctttcttc
      781 tcttcttgga acttgtttat ctccaccgtc agtttctctt cctttgctgt tagctgtagc
      841 ttttcatgaa taagaaaaaa tgtactgcct acaccaaata agaacaatcc gcctacacaa
      901 acataaataa atcgtttctt catttgacac ctcttatttc tttatcggtc gagcaaaaca
      961 aatatcacca ccattaagca aatacccaaa ggataaattg acttgccctt cacctacacc
     1021 gttagtattc attccccctt gttcgattac tttcgtagaa agtccatgcc aatcttccgc
     1081 taaaacagct gtgtgtccat catttcccac accagaacct aagttcacaa tgataatatc
     1141 gccagctttt gcctcgctct caggtatttc ttgtaagtat tgcttacttc ctcttgcgtc
     1201 gctcgtcatt gagccggtaa accacccaac gttcgcaggc gttttatacc ctgctttcgt
     1261 taaaacaagc cacacaaagc tcgaacaatc ggcatagcca tttttatctg gattttcgac
     1321 actaccaaat tgtgcccgtt gcggttgtga ataatgaaaa taaccaatca gactttttgc
     1381 tacgtttaat acattactat cggaagaact gcttgttcct gttgtttcta gacaataatc
     1441 ggtcaagtct ttacttcctg cgtttgcccc attttctaat gtaattgaag cgacgggatc
     1501 actgtcattc tttcctagtt ttgcgtacca ttgattcgca aaagtaatac gattccccat
     1561 cattggtatc cctgcacgct caaatttcac ttgccactgt tctgttgctt cctctacgct
     1621 ttttgcttgt aatgcaactt gtgaagcttt ttctgttgtg tttagttcat gaatcaaaaa
     1681 ctcaacttgt acacctaaat ctgaccactc tttgtttcgc tctttggcaa aatttttcaa
     1741 agcagtcgtt cgccctgccg tccattgtgc aatcccatgc ccagataatg gatcgtttgg
     1801 tcgttcaatc gctgtgggac tcatactcga ctcttgttga aaattgccta acatacccgc
     1861 tagaccctct aatgtcgtat ttggaatatg tttttgtact tcctttgcaa tttgaagccc
     1921 gtttgtctcc atgccttggt tcgttaatga gatagtactt gcgacaattt cggttggact
     1981 acagtcgatt tcttcctctt cagaaccact actaatcaaa agtatcataa acagaaaacc
     2041 aaacatacat agtaatagtg tgggtagaca accgaaccca caccataaaa gaagtttttt
     2101 cttcatgttt gatccttcat tcgcttagtt cattttttgt gttgcttttc ttttgcgcca
     2161 aaaagcaaaa ccgcttcctg aagccaataa tccaccaagt gctgtcgcaa cgagagacac
     2221 ttcttcgcca gtcttaggca atactttcat ttttccatca gaccctttca ccgcaatatt
     2281 tccgcttgga agttgttcga ccttgtctga aatgggcgtc aagccatttc ctgattcttt
     2341 ataagcgaca ccatcaacga cggagacaat tttttctcct gaagctgttt caattggtgt
     2401 acttggtgtt ggaatatggg ttaattcaga cgcattattg gtttcaattt gaacattttg
     2461 tccacctgtt tgttggctgc caatacttcc atctggtttt actacgacgt cctgtactgg
     2521 aggcgtagtg ttcgtccccc cattattggt aggtggtgtc gttggaggag tcgtatttcc
     2581 ttcattgcct cctggattcg acggcttttc tggtgtctct gtattaccac catttcctgg
     2641 gttttcctca acgggtggaa cttcaggtgt tccttcccct ccgccagtat ctggattttc
     2701 tggtacttca ggcgtttctg tgcctccacc tgtatctgga ttttcttccg ttggtggaat
     2761 ctctggggtt tcaggagttt ctggtatttc gggaatttct ggggtttctt ctcctccatc
     2821 tgttggtggt aaaacaggtt cttcaggaac ttcaggtgtt tcttccgttg gtggaataat
     2881 ttctgtcggt ggtacacttg gatcacctgg tgacgtagta tctgcatacg cactggttcc
     2941 taccatacct agcaatacag ttgcactaaa taaacttcct aaaatcattt ttttcatgtg
     3001 tttttcctct tctcttattt attgatttat ttcaaaatca tctagttcgg gaagcgtgga
     3061 aactagataa tttttttcct gttcttccaa ttgaaaagac actttttttg tttcttgtgt
     3121 ctttttctcg gtttccaatg taatgacata cgcaatttgc caatgcttgc cttctttctt
     3181 catttcccag gataaaatac ttttgacttt ttccttttga ggactgattt gtttcgcaac
     3241 tttttccgtt agaaacgatt gaagcttttt cctataccgt tcttctgcca tttcacccga
     3301 ataataattc gtaataaaat aacgactaaa tgtatcaatt tgtggttgtt ttgtcgcaag
     3361 ttcttccgca aatgcaatgc gttcttgtaa ctggtgctcc tttttcgtta gttgttcaat
     3421 ctctctttgc gttgaaagtt gcataataaa aagaccacaa gatacaacga cgataaatcc
     3481 aaacaaaagc cattgaaaag acattttttt cttctgaaga gagccgttgc tttgtgtaaa
     3541 cgctgtttct acagaaagtg gtgttttctt ttcctttttg atttttgttg gtcgacgttc
     3601 gttcttttgg tctttcttta cttgataaaa cttcggtaat tcggaattta aggtttgaat
     3661 aaaggtttgt gcttcttcac ttaattgccc attttctgtt aaagattgtt caatcaatga
     3721 aatcaaatga aactcttcta ctggaaaatc atacgagcct tcaaatactg gaatttcatg
     3781 gttttctgtg tagatttcca aggtacacca gccaatttcc gctcgtgcca atagctcttc
     3841 attttctctt ttcaattgaa taaatgcctt atctaatgca atttttttat ctgttagtaa
     3901 agacatgtta tcaacttctg taaatcgaat catcatcatt agtcaaacac ttcttccacc
     3961 acttctaccg catacaccac ttgaataggg ttatttaaag aaaatttcac ccgatattct
     4021 tgattgttca aactgacgat atagctatcc tcatcttcag cataataggt atattcgttg
     4081 cctttgactt gaaaatattt ttggatcgct tgtctttgtt gccattcgcc aatctttgga
     4141 aaataggcga aatgatagcc aaataaaacg cctatcccta ataaaacaat tcctgttact
     4201 ataatcatct ggtttcctcc tgtacctgtt tatcttctat tgactctttt gtcggtaagc
     4261 aataaagaac cagtaacaca agagagccaa tcgttgggac aaaatagaaa aaaataaatg
     4321 cccaatgaaa acctgcgtct cgtaaacgtc gaacgctgag agaaagagac gggacaagaa
     4381 ctacgaaaca atacacaatc aatacgccta tcaaacaacc aattattgtt tcagaagcat
     4441 tcaacgctcc tgctagtcca atcgctaagc ctaggccaaa cccaatgagg gcattcatta
     4501 acgcaacgag ccaatactct tttcttgtgg ctttcccttt gtaattaaag ctattctgcc
     4561 aaaatttttt ataatttgtg atcatatctt tgtcctttct aatgtttatt tttgattcta
     4621 gtttcttctg ttttactaac aatgaaactt gccataaaaa aggtggccaa tacaccacca
     4681 gctagaaatc cacttacaaa taacaaaatt ttttcctcct ttctaaaaaa tttcgccata
     4741 aaaaggcaaa gtatgaacac gataaatacc atgtacatac tttgcctttt tatgcaatat
     4801 atgttaactc tccaagcaat tactgatttt taaattcttt ataaataaaa taaaaatcgt
     4861 ttgagtaaag aaataagtca atgaagaccc aaacgataaa aaatattcct aatactatat
     4921 ttaataaact acgttctaga tagatacgag atttcaaatt aaaaaacgaa aatgagagat
     4981 acaaatatat caaatattta aaaagtaacc acattttcta taccactcct tatcagaaac
     5041 agccttattc tttagaataa gactgtttct gggttgatta atttgtcttt tgttaaacta
     5101 ccatctaagg caagttctaa atgtaaatgt ggtcctgtac tatttcctgt tgaacccaca
     5161 taaccaataa tttgtccttg ttcaactgtt tgtcctacgt tcacgcaata ttcctgttgg
     5221 tgcgcataaa gagccacact tccgtcttca tgctcaattg tgacataatt tcctgacaaa
     5281 gtatgatacg aagcttctat gaccgttcct gccttgctcg catagattgg ttctccttga
     5341 ttggctgcca tgtctaatcc tctatgaaat tcttctcctc gccaaccgaa ggaactagaa
     5401 attgaataat tttttagagg taccatatag ccttctttgc ttagttgtgg accagctact
     5461 tctttatcat atttgattaa gtcataggtt tcgattaacc catttaattt tttatcgtag
     5521 ctagtgtctg ttgcgtatct tcctgttaaa aatttagtcg cttcctgata ggtttttgcc
     5581 ttacttttcc aaacaccatc ataaaaagag gtatttccaa ctaagccttc ttttaacagt
     5641 ttggcatagt cttttaagct ttcttcatag ctctcatatt gccgaaaagt tgcttgggtc
     5701 gtataaagtg tcccattgcc taaatcttct tgtgtcgcaa aagagacgcc ctttccgtta
     5761 tgtgtgcctt tgattccaaa tagattgtag tttggtgcct gagccaattg actttgtccg
     5821 cttgcggatt ctaaaatggc ttgcgcgatc ataacagaag cgtataaatc attttcttgt
     5881 ccaattttac gagcagattc accgatttta cggataaacg tttctacaga cgcgtctttt
     5941 tcaaaatgaa tagtcccttc gttatcttgt gtgctggaag ggactaattc ttgtatcggt
     6001 gcgcttggtg cttggtggta gttttttgtc tcttctgaac cgattgattg tgcctgcggt
     6061 acgtcattca taggtacagt cacactctgt tcaggtgcct tttgtgggcg ttccgttttt
     6121 gttggcggtt ccgcttctgt ttcggtactt ggtttttgag gagtctgtga gctttcatca
     6181 gaaggttgct catttggttc ttctgacggt tcctcctcag gttgttgatt gtcttctggt
     6241 ttgtctggtt gattcggtgt ttctggttgt tcttcagaac ctgttggtgg agcgttcatt
     6301 tctggattat ctggtgtatc tattgtatct tcagtcggtg tttctggttg attttcttct
     6361 ccagtggaat ttgttgtcgt ttcttcagtg atcgttgcag attgcgaatt tgtcagttga
     6421 ttgttgcctt gttcattagc caaaaccgct ggcgtatacc caaatacttg acctactaat
     6481 aacaaagcag ataaacattt actcgttttt ttcacaagat tgcctccatt cttcaatcaa
     6541 tcatctagag acagctgatt gctgtctcta gtcatacttt aattgctttg tttcatttta
     6601 cgacgaacca gaacagcccc cacaccaaca aaaagaaaca gtcccaatgc aattaacaca
     6661 tcgttcactg tttctcctgt ttttggtaag ctttgttttg gtgtgctcgt cggcgtttgt
     6721 ttgacagggg ttgtcggcgt ttctttttta atgccttttg ctgtcaatgt ttgtttttca
     6781 ttcttcaaat caaagtgacg tgcttcttcc tctttatggt ctttatcctt atagacaata
     6841 tgagttgcga ccaatttctc cccgtctttt aacgtgcctg ctttgtagtc aaaggatact
     6901 ttaaactctt cagacgcttt agaaacttta cgtgcagttt ctaccgtacc aacgactgta
     6961 tcttttccat ttttatcaat cttatggaat tgagtcaccc agtatttggt ttcccctacc
     7021 gtagattctg gaaattcttg cgatacatga tcgatcaact cttggtcttt gtctggatca
     7081 aatgtttggt cgccattcac tgtaataaag cgtgtttcga tttttggttc tttcgcagtc
     7141 actgtttgat ctttattggt taaatcaaaa tgacgagcga attcttcttc ctcttctttg
     7201 tcgttataga caatgtgcgt tgcgacaatc gtgtcaccgt ctgaaaccat gtctgctttg
     7261 tagttgaacg tcacatcaaa tgtttctttt tcatcggtca cttttcgtgt ttcttttacc
     7321 gtatctaata cagtgacttt tccatcggca gtaattttat ggaattgcgt cacccaatat
     7381 ttctctgtcc caaccgcaga tttagggaat acttgggaca ctttatccac taattcttgg
     7441 tcttttttcg ggttgaaatc ttttccacct gtcgttgtca caaacaaagt agaaatttct
     7501 ggttggtctt cttctggaac cacgatttct tcgtttttca cagtaattgg ttgtacttct
     7561 ccgtctttga ccgttactaa aatcgtttct gtttgtcctg acgcttgccc ataatgctca
     7621 gggaacacaa tttccgtaaa tttatacgta ccgtctggta attttagttt ttctggttct
     7681 tcttttgttt catacacaaa ggacacattt ttattatgtg cagtcatacc ttcaatcagg
     7741 aatttcgctc ctggtaattg tagatgattg attgaatcga ctttattgaa gtctacttca
     7801 cccgttttta acaagttgtc aatcacatct aattcaaacg taagcacagg cgtcgtgtta
     7861 tcgccatagg ccacagagaa cgtggtttgt tcttcagaag gttgacggta gttgtctggc
     7921 acattttttt caatcaattg gtatttgttt tgatcgccat tgtagaattt tttgtttgcc
     7981 ttccagtgtc cgtctttgtc agtttttact gaatccaaga cttctccttt tttcacaagg
     8041 actgtttctt tatctggcgc agtaatatct gttttggcga taatatcaaa ttcgatattt
     8101 tctgcaggaa cggtatcata aacaggtttg ccttgattcc aggattcttc cgcattatag
     8161 acttctttga ctttctttcc ttctggaatg aactgttgta agtcattttt tgcagttaac
     8221 gtcacttctt tgtttggttc aatcgtaatc gtatgagttt gtttgtctag aatataccca
     8281 gctggtgctt tgacttcttt gacatccaat ttattttgaa catattttcc gtctttttca
     8341 tagaatgcag ggatttcttt taaggcgatt tcccctttgg catccgttgt atattgtttt
     8401 gtttctcccg tatcatgacg tttgatttca tacgtcacat ttggaacttt tttccctgtg
     8461 acagcgtcta gtttggttag tttgaagtcg ccatttttta gaactttaat agttagtcca
     8521 gctctattag gatctggcac ataaagcttt cctagatttt gagcaacagg atctacatag
     8581 gcaaaacttg aacctacccc tctagaattt ggatccagtg aagaaacaga aattcgtccg
     8641 tcatttgagt tttgatttgc ttttataaac aaactatttc ctgattttct tacttgaacg
     8701 ttagctgtgt tttcaacaac cgacccatcc cattgagaaa acacaccttt tgtatcctta
     8761 acttctactt cttcccctac tttaaccgtc actgtttggc tatcaaaaga aggatcttca
     8821 agtaaattgt ttatttttct atctacagtc tgtttgaaag cttcataatt agattgtcca
     8881 atagttccat aattaacaaa tgaaccacct aaaacttccc aaatatatgt ttgtgctaat
     8941 acatagttat ccattgaacc actagaatag tagccaaaat aaccaattaa agaaatcttt
     9001 tttctaatag cacttgtcgt aattaaattt tcgagtggtg tcacatcata ttgaataccc
     9061 gcactcgtga ttgcgccttc ttgaatgcaa aaaacatctt ctccgtcaat ttgccagcga
     9121 accatgtaat catgagtata cattgttcct tcaggagtaa ttcgagtcat attcacatct
     9181 ttacgaccat tttcagtcaa tgttagattg gtagcacgtg tctgtgcatt cttttccaag
     9241 attgcttgcc attcttcaga ttcttgcgcc aatcgttctt gttcagaaaa agaagtcgct
     9301 ttttgaatat cttcaatggt tataaagtat ttctcataac cttcagggac ctcttgagtc
     9361 attacattgg gcggagaaac atcttctgtc gtgtcctgcg tagttgtctc ttctgttgtt
     9421 tcacttgtgg tttcagtact gatcgcttct tcactacttt ttgattcttc agtcttcatt
     9481 acttcttcag tagaatttga ttcatttatc gtttctgcaa atgccgttac agatcctaac
     9541 atgggattga gaaatgtaac gaaaattgcc aaccaagttg ccattctttt aaacatattt
     9601 ttttcgttcc tttcatttta tttttcagtc ttctaaagta atttcttatg gacttcgaca
     9661 ccgaaacatt tttatcctcc tcaaaacaaa aaaagactag aacttataat tctagtcttc
     9721 atcaaccact tacttttctt ttatttttag tagaaatcta cagtgtgttt ttgtgacccg
     9781 tcactgtaaa taataacatc caccataaaa cctttgttgt accactgcga atttggatcg
     9841 ctaacctctg cattagccca atcataggct tcatctacag tgttaaatac ctttccacta
     9901 ttaccaatct ctccaactac aattgttggc tcttcaggtg tcgttgtgcc accatttccg
     9961 ccttgatcgc ctccaccagg attgcttggt tcttcaggcg ttgttgttcc tccacctgtg
    10021 cttgggttag aaggagttgt ttctcctcca gtatttccac cattacctgg ttgtgtaggt
    10081 gtttgagtag tgccaccatt gttttctggt gtacttggcg ttgttgtacc accagttgtt
    10141 cctgtttgat ctccagctgg tgttgttggt gtcgttgtat ttggtgtcgt ttgtgtattt
    10201 gaagtcgttc cttgtcctac tggttgattc ggatcaactt tgtctttctc ttgaatcact
    10261 gtttgagcca cagtttcacc tgcgctgttt tgtacacttg ctacattgcc attaaatact
    10321 aatttatttc ctgcatagat taaatccaca tttgtaatct tattcatgtc cgctaatttt
    10381 tggacagtaa tatttgttgc ttgactgatt gcgcttaggg tatctcccca gacaattgtg
    10441 tattcattcc ctttaatttc tgctttgatt tgttccgccg aacgaggtgt ccaaactttg
    10501 ttttctgctg cttcagcatt cgttcccaca gcaagactaa ctcctccgac tacgattcct
    10561 agtgccaata ctttcaacat tttcattgtt tttcgcctcc gatttgaata aatttattta
    10621 caaaaacata gtaccaaact tatgtctatc agtcgatata ttttttgtag atttttttat
    10681 tttttgatat actaactttg gtactaaaat aactaaaagg aatgtcacac atgcaactta
    10741 ataatttaaa tgaaatacat gattgggctc aaaataattt acttaatcgt aaacaagctg
    10801 ccaaaatcac tggacaaaac tatgatgctt tttctagctc tttacgtaga aataatatca
    10861 aaccatttct agaaataggc gacgaacctg gtccttccat cattcgctta tatctaagag
    10921 aagatattga aaaatacgca aaacagctcg aagaaaaaag acagaaacgt caatgaggcg
    10981 ttttctgtct tggatttaca agtaaaacaa taataaacac ttttacaacg gaggttctat
    11041 gaataagaaa tacattgttt cagttggttc aatcgtactg attgtactcg ctattattgc
    11101 tttttctgta tccaacaaaa cagaaacaca gccacaaaat acggttcaat cgactactac
    11161 aagtcaatct agcgatcatt ccgtctatcc ttcactaaat agtttaaaag aaacgcatgg
    11221 acaagattta attgatcttg gttcttctca aattactact gtcttatttg aaaagaaaaa
    11281 tattgcgttt attactccta aagaaggaac tgaccttgaa ttttctatca aagtaacaaa
    11341 taccaaagaa aatttattct ccattcctga cattcaatat acccctaaca agttaaagat
    11401 tggggatacg tttggtttgg ctaaagtaaa tgaacaatac tttgcttact cactgaaata
    11461 agtttctaat agcaagagga aaaaccctct tgttattttt atcacataat cattttataa
    11521 aaacgcttcg aaaatatcaa catttttgaa gaaactattt ttctaatttc tcaatttctt
    11581 ccaagggatt ttcgctaatg acccatacag gcacagattg ctctcgaggc tccccttctt
    11641 ctaaccacca acctgcataa tgaacttctt cctcctcagc attaccatga taccaaccaa
    11701 caaaggattc atctacaacg aatgaaattt cttcttctgg atcaaataat tcatttttcc
    11761 atgcttcaat tgcttgttct ttggtgtact tttgtttgtt aaatacacca caaatttcgt
    11821 catctacata aaaaatttca aaatcaaaat tactcgttcc ttttactggc atcttaactt
    11881 ttcctccttt gactcacttc aatagatata ccactcatgt tcattccatt gtttactagt
    11941 taattggagt tttatcttag ggctttgtgc actctttcca aaaacttgag cgtcgttttt
    12001 ttcttctaac caaaaaaggt aatttcttac attcggtact aaatgttttt cctgcatttc
    12061 ttcatgaaaa acatataatt ctgcttcatt tttcaaccaa ttcattattc attccctcat
    12121 tcacttcttc ataccgccat tcagatagtg agatttcttt ttgtttctcc acttgtccta
    12181 taaaaatcgc tgtattcaac aagctgatca cacttaataa taaagcaatg aaactaatgg
    12241 ttacacttac tctttcacat tttgtcattt tgtttccttc tttactcgtt tgttatttct
    12301 tgtaataggt ctaataactc cttcaccgaa atttgcaaat ggttcgcaaa aatttcaata
    12361 aaagcactcg cacacattac cagaccttca actgatatat cactgcgaca ataaaattct
    12421 ggagaaacac tgatttctga ttctttctta caattgaacg aaaaaactat ttttgaatca
    12481 aattccggta attcttccac aactatgttt tgggattcta ccaagaaagg gagtgcctgt
    12541 tctaatgctt cttctggtgt ttgagcagat agaataattg cttcttgttc ttttatttca
    12601 ttgttctcaa atactttata actttttttc attgttagtt cctctttcat tcctctctca
    12661 attagctaag ccgttactgg tacgccattc tctaaattta gtatcgctaa tccttaatgc
    12721 tcgtcgaata tcaatcattc gataacctat tgcacgaagt tctttatatc ttttagcagt
    12781 tagcgtctct aacgtcattt caggcaatgg gttcgaatca aaaatagaat tcgaaccgat
    12841 tatttgggcc aattgttgta cttgtaacag ctcttctgaa cttaattcag tgattgctcg
    12901 attgcctatc attgcataca ttttctgtcg aatatacttc ttttcttgtt tagacaacat
    12961 ttctatccac tcctattttc attatcattt gttacctcca cttcagtaaa tccacaaata
    13021 aatgaattca tattccaagt accattccct tcaactacaa cttttctcct gtcctctttc
    13081 ggatattcaa gaatcagacc gtttaccaat atgtttttca ctactactaa tttgtttgta
    13141 taccgtggaa ttgctgttcc gatatacttc actttatttc ctacgctaat tgtcatttcc
    13201 tcactcctat tcttttttat ccaaaacttt ttcctgtcct attcctgcca acgaatatac
    13261 ttcactgcat gcttaaacaa aacaaaatgt tgccctgttt tatttccgtc ttttacttgt
    13321 tccacataca gttcatattt ggtcacacct tttaaaacgc cagatacttt ttttcatcca
    13381 ttgtcacaat tagttttttc atcaatcgat tgatactgca ctgctcattt ttcttgttca
    13441 tatagtattc ctttcttttt caaaactagc ctcttttgtt tactagtttt catcaatcat
    13501 caacgattta acagattttt tctttcgcag ggtttctagc gtcagcaacc ccaaactcta
    13561 aaaaagtgcc tttgctccct tgaaaaacga gcggacgatt gtactcgtca tcatatgttt
    13621 ttcatactac ctgatccacg caaataacct ttttttgttc tgtgccacac ccatccatac
    13681 gtatcgcttg agattcacca tactttttta ggtggtttat ccttcctaag tccctgttag
    13741 acacgctgat taacttagaa agtcctgttt gtatccaatt ttcaaagatc tcccaaaaga
    13801 aataagtagg tttacgtgta tcgcttctgt tcaaagtacc tgtttgtact tgagtatttc
    13861 tactcccctc gttcaaggga ttccttccac cagctagctt ttcctttcat catcaaatgt
    13921 tcctgaaaag catttacgtt tcgtgtccgt gtgcttactt attctttttt cgatagcgat
    13981 tatccgctcg ctactcgcta ttttatactt tgtttgttag ctcaaataaa aactaacaaa
    14041 caaaaaaggc tagtttcttt ccgttttatt tttttaactg tcgataaata gttgttagtt
    14101 taattccaag ttctaaaacc aatacgccca acaaaaccac aggaaaaaca attggggcaa
    14161 acaagtaaaa tcggatagtt ttcatgtctt ccctctttct ttttggtgtc gctgtggtcg
    14221 ttcttgccgt tcaatacttc gatacatttt ttattcatta tttgctcgtt ttgttttcct
    14281 ctggactgct tttcttgctt tttgaatact ttatacgctt ttcgtgcctg aattaatagg
    14341 tcttgtcggc gtttcattcg tgagaaaaca atatttgaaa ccatcaaatc ctggttcgaa
    14401 caaccacgat tcgcaataat tttcccatca aaaaaaatag ttttcacttg agaaaaaaac
    14461 agttggaaat cttgttttgt tgctaatttt tttgtcaaaa tcactgtgct tttccctgtt
    14521 gaatctacaa tgattcttcg aacaatccga ttcgataact tccaacgttg ccgattaaac
    14581 aatgttactt cacaacaaat atgacgtaag tgattagggg tcattttcat gtgtccaatt
    14641 cctccaacca attatgcatc gaaattttgg gcaattgtga cagcccataa atctgcgttt
    14701 ccaggtcttc ataactacaa gaacgcttgg cttgaagtaa tgaatattct gcttgatctg
    14761 atagttttgt tgccattgga tctagattca attctcttac acaacattcc caaaaattca
    14821 atagcgctct aaaataaaat ccttttcgat tctgaattgc ttttccttca tgacgagcaa
    14881 ttttttcatt ttgaatacac cgttgatacg ttttgcttaa atcttctgca aatgtttccc
    14941 ctaaaattcg atactcgctt ccatcagata aaccatgtgt ttgattatac tttcgttgcc
    15001 gcttttctat aattcgttta ctgtcgtata tcatgtttag ctgctctttc gcttcacgcc
    15061 attcagctga actgcgaaca atccatgtca aaacatcttt cggaacaaac gtactttcgc
    15121 tgtaatgagc aataaactct ttttgccaat catcagccat ttcaacagga ctagtcgtat
    15181 cgatttttgt atctagattt tctgtatcta gattgttaga agaattatta aataattgtg
    15241 cagaattttc acatggattt ttttcgtcaa aatcatcgaa aatcgctaaa ttatcaacag
    15301 attcctcctc ttcttttcct tgtgcagaat ctacactggt tgagtcaaca ggacctaatg
    15361 aatacacatc attttctgtc gtatctaatt ctcctagata caaccgagct ttgtttcttt
    15421 ttcctgtttg ttgatcaaat cccatatcct ctatttctag tagctcatat tctttcaatt
    15481 cttttttgat tgtattaaca gaacgtctgc aacaattcaa tgcattggct aattcttgtt
    15541 ccgtaaattc aaaataaaca aaaccttgac tatctacttg atttcgttga atggctaaat
    15601 ctaagcgtga ccgaaaaacc atatacgcga tttttgcttt atcagacaat gagcgatatt
    15661 tttcacttgt aataaatacc tttggaaatt gatagtataa ttcattgtat ttacgagttg
    15721 cggtaaatct ttttgttgcc atcgttatta cacctcatct tgtccatata ttaagaaaga
    15781 attttggtgt tggtagacac gaacatcttc cattgtagca atcaattctt ttagataaat
    15841 tcgaccgaat tcaattgtta gtaagtcgta atcttctaat tcatttttga tttcttcaat
    15901 ttcttcttca gacacattga gtaaaatggc taattcttga ttggtataag aaatatagac
    15961 atgtttcttt tcatcaatcc gtttttcaat taaggattct tctaagtact cattcagtaa
    16021 gctataggcg tattttgctt gtgcagtcat cgcataatat tttgacgatt ttataaagaa
    16081 tagttttggc atatgaaaag ttgcttgtgt cagcacatct gttgcgcgaa atttgttttt
    16141 taccatattt tttcctccta gaaaaacacg caaaaaaagc tctgtataaa tcgaattaca
    16201 cagagtttct tcttgttgat atttaatttc taaaatacgt ttcgggtaaa aactaaacac
    16261 ttactaatat ttagattgta gaatcttcgc cttcatttat tcattttaaa gagaaaaaga
    16321 tcaagtgaaa tgtgcccaaa agtttgattc tattaatttt taaacaccat tcataatata
    16381 tttcctataa tgtaaaaagc gtcgatactt attaaagcat cgacgttatt tttatagtaa
    16441 atttatcaaa atttttgcca acattttttc cgattttttt ccctatttct atctttcctt
    16501 atctttctta gttaatatta tttgatgtta atatatcact actaatatta aaaaacattg
    16561 atttatcata ggataaagag tgatagttta atacttaaag agacatatta attttattta
    16621 aaaattgaca ttaatgactc ttaatcagcg ggtcgcgggt tcgagcccct cacggcccat
    16681 tgggtgccaa acccacggaa caggtaaatg ttggtgcgaa agcatttcga aaacatttac
    16741 gctgttcttt ttttattttt caaaataact agggcttttt atttttaact caaagtccac
    16801 tgtagccctt tggactttga gttaaaaaag aagcataatt atgaacagta aagtttctaa
    16861 tataacaata attcgagaag ctttgcaaaa tcatgctgcc aatgaactct ctaaacatat
    16921 aggattaaat ctaagtacta taaaaggtta aaaagtgaag aatatttaat agaaaaacta
    16981 aatttacttg ctgctttccc cttttccatt aatacaacaa tcagagagtg tctttttttc
    17041 gaaaaaatta gaataaataa aagacacgta gtttttttat ctacatgtct tccatcgact
    17101 acaagttatc atttctcaaa acgtatttag cttttaattg attaatcgtt tcacgttgtc
    17161 tacacattgc ctaattaaga ttttcttatt agaagcatca atcccgcaat aatacctaaa
    17221 actccaggaa ttacccacaa taatgttaaa agaaaaaatg gaataataga taaaatgagt
    17281 aaactaatac catagaccac tgttttttga ttttttctta ctaaaaaacc tattatgaaa
    17341 atcattatcg aaaaaactag ggtaattcct ccaaataaaa caacattccc gcttagattg
    17401 actaacatat ctatatcctt actggttagt tctgtagtat ttaaagtagt tgaatcaata
    17461 gaagatagaa acataccacc catcaatgct aaaagactac ataaaatccc tgttattcca
    17521 ctaattaaaa tcaaaataaa ctctgttttt ctcttattca tttaattctc tccagattaa
    17581 gtgttttaga attagaaatt ttttgaccat agttaatagc ttctaatgaa cgaataacaa
    17641 ccactgacgt gttagaaaaa taatccccaa tatgttgttt cttgggcgtg aaagcagcca
    17701 ccaaataccc aatttgaaat acaaaatttt ttaatataaa cctacatata cactcacgaa
    17761 taaagatagt ttgccaactt agctcttttt cctcaaagct cactactctt atcccaaaaa
    17821 tcattttccc aattgtttgt cctgaattca gcttagtcaa aagtacaaaa taggttaaat
    17881 aaatagctaa tgacgctata ccataaaaag taataatgga tttgtcagat tccagtccca
    17941 tgattttgta aaagagacct atagtagcat gggtgatgat gctaatacaa attaaatcaa
    18001 taagaaaagc aaaaaatcgt aaccaaaatc ccgcaaatac ataattaggc aaagtttcaa
    18061 tgccactctt cttattcatt ttatagccct ccatagtaat acattggttt tggagtatca
    18121 gaaaatccaa gtttttcaaa aactgaaatt acacgatctg atgtagttgc ctttagccct
    18181 tgccattcag caatcttatt acctaaccaa gaagaagcaa agccagttgt gtcattttta
    18241 tattctataa ccgtcgcatc agttaatttc ttctcttttt ttagggaatc taatgcatct
    18301 tcagagtaac caatttgatc caccaaccca ttttctactg cttggttacc atcataaatt
    18361 cttccatccg caagttcttt cactgcatta gtatccatat ttcttccttt tgctacaaca
    18421 ttgacaaaac gatcataagc actcatcaca aattcctgta taactttatt ctcttcctct
    18481 gaaggtttgt gttgaggtga catcatatct tttaatgcac cacttttata cgtcgcatca
    18541 gttacgccaa gtttttctaa taagccagaa taatttaaac cagaaataat gacaccaatt
    18601 gatccagttg ttgtttcttc cgtagcaaat attttatctg aaccagcaga aatgtagtac
    18661 cctccacttg ccgctgtatt tttaaatgca gtatagatag gaatatctag ctttttaatt
    18721 tttgccattt ctttagcaat ctcagcactt tcatatattc ctcccccagg tgaatttact
    18781 tctaataaca ctcccttaac cgctttgtct tcttgaattt tctttagctg tgttaaaaaa
    18841 ttctgatggt tatattgttc cctactaaaa agattgcttt cacctgtatc tgcaataact
    18901 ccattgacgg atagtttgac aatcttttta tttgatgcgc cttcttctaa cgtttcttct
    18961 gtgatttcgt tagatccgta aaaaatctta tttattcctg ttagggttgt atttgatgct
    19021 tcctctttat cttttcctgg aattagcgcg acgataatgg atagtaacaa taatccgcac
    19081 gctactccca cagcaatcca tcttctttta ttcatataaa ctcctcctat attcttagta
    19141 aaaataatta ttttttattt agaaacacat cgacaatttg ttgccaatat gttttttctc
    19201 ctacaattaa agacaactct ccagttaatc cataacgaaa cggaaacgat tgagttgttt
    19261 ttaacgttcc tttcactgca taaaaaacac ctttctcagt ggcagtagaa ttttctgaaa
    19321 tttcatctaa agttccgtcg atcgtaatcg gagccccacc tttttgatcc aatttaaaat
    19381 gaaccttcat cccttcttta atataggtac tttcattcgc aggtaaaagt gctgtaaatt
    19441 tcatttttga tttttcgtgg atcggataaa tttctgcaag cactgttcct ttggctagtt
    19501 ctgcttgtcc ctttgtttct tcatttaaat gaaccgttcc agatacagga gcttttagta
    19561 ttccttttga aagttgatcg tccaaacttt tgataactga ctggttcttc tcttgttttt
    19621 catttagctc tgtaactgtt tgttttacag ttgttattgc ttgttctagc tcttgctttc
    19681 ctttttccgt ttgactaatt ctgtcattct cagaagtagt tggtttaact aatttagctt
    19741 tttctgactg atactgttct acttcttttt ttagttgctc aatttgttca tttatgcttg
    19801 ctgaaattgt cagtttcact tgattttttt gatcatcaga aacattattt agttgttctt
    19861 gccaattctc atagtttgcc atcatttctt ttgaaaaatc ttttaatgat tgattattac
    19921 tccaagctac ctgtacttgt tgccattctt gtaatttact atctgaactc tcaatttgtt
    19981 tttctagttg tctttttgtc ttttcgtata cttctaattg cttttgatca tttaattcac
    20041 tttgcttcaa agcgtagcgg agtgattctt tttctgacaa catgctcttt aattgattac
    20101 tgtagccaaa actgtcatcc gttgaaaaca aattttttcc tttacttata ctttcaataa
    20161 atagctgagc catctttatt tgttgagtaa tacgttggtt ttcttgttct atctgagcct
    20221 tttcattctc tattaaagaa caatccaatc taaccaacac atcgtcctta tgaacaaatt
    20281 gattttcttt taggaagttt tccattattt tagcttctaa tggcacttgt aacttctcta
    20341 ttttttcggg gttcaattga gccgttgtcc gaataacaac ctctttttta gcaaaggtta
    20401 aaaacaatcc aataatacat aaaaaaagaa ttacagggta taacacccaa cggtagaagc
    20461 tatgatgttg tttagtatag atactagaat gttcgaacca attattattc tgcataaatt
    20521 gtttccctct cctagatttc ccataatttt tgatagatac catcggcaaa acgaagttca
    20581 tcgtgcgttc ctttttctac caaatgccct tcatggagaa ctaaaatttg atcacatgct
    20641 tttgctattg gtaaatgatg cgcaataaaa ataatcgttt tttcttttaa attgaggaga
    20701 taattcataa ttgcatgttc taacaaggta tctaatccac tagttgcttc atctaaaatt
    20761 aatatgtccg gattttttaa taaagcccgt gctaaggcta atcgttgtcg ttgccctcct
    20821 gaaagattat ttccaccttc ttccaacaat gtctcgtaac gtaatggctg ttgattaata
    20881 aattcattta gttggactat ctcacaaact tctattattt gttcgaaact tggtgggcta
    20941 tctaaaccga atataagatt ctcaatgatt gttccactaa agaaaaatga ctcttgaggc
    21001 aaatatgtca ctcgatcgcg taatatctga aaaggaatgt ctaagtgatt aatgtttcca
    21061 taatggatcg atccttctga tgcttcataa aaacgaacaa ataactttgc aagagttgat
    21121 tttccagaac cactaactcc aactatagcc gttttactgt gcgaaggaat tgtacatgaa
    21181 atttcgttca acgttggggc cttcatgcta tacgagaatg aaacgttttt tacagtgata
    21241 tcttgttgaa ataattgttc agaaagcata tattcaaaag actggttcga tttttctgaa
    21301 tcaatcgcga aaatttcatt taaacgatta tttgcaactt gagccgtttg cattttcact
    21361 tgtaaattaa taatgttttg taaaggatct gtcaaaaata ctagtaaagc attgtatgtg
    21421 ataagttgac ctagactaat tctattatcc ataacaaaat aagaacctag ccataaaatt
    21481 aatgcactgc taattaattg aatcaaatgt ttaattcctt gttgaatatt atcaagcgta
    21541 accgtacgaa atgatttttt cattaattta acaaattcat ggtctatacg gttatatact
    21601 ttctcttcac cgctatatgc tttaattgtt tctattcctt taagactttc tatgatacta
    21661 gaatttacag ctgcaccggc tgccatttct tccctgttcg atttttcata gcttttcacg
    21721 aaagcgaata ttgcaactac ataaaaaggc aaagagcata gagtgattaa aaacaacgta
    21781 ctattctgaa tagctaacgt aaccccaaca agcaacacca taccaatatc taaaaatatt
    21841 gataacgtag cacttgctaa agcatcaata atcttactgg catcaagaaa acgagaaata
    21901 atttcccctg attttctcgt agcaaaaaaa ttcattggta aagttaacac atgtttgaaa
    21961 tactgaagca taattgccat actcattcgc tgtcctaaaa taataagaag ataactacga
    22021 ctatattcaa acaatacccg aaataaatag acagcgatca agccaatgga aattatattt
    22081 aaagttgaac gtgcttgatt agggatgaaa aaatcaagaa taccttgaaa ataatatgag
    22141 cttcctatgc caaaaaacgt aatgaaaatc gatgctaaaa cgatgtggaa tactaatcct
    22201 ttttgtttcc aaattatagg caaaaaagaa cttaatccag ctactttttc tttaatagga
    22261 atataagttt ctctaggcat aggtaataaa agaatacctg tccattcttt ttgaaacgct
    22321 tcaatcgttt ttctattttt tccattagcg ggatctgcaa tccatagtac gtttcccttc
    22381 gttttataaa ttacaacgta gtgcatatag ctgttatcaa ccaatatatg tgcaattaat
    22441 ggtaagataa gatctctttc tttccagatc tcactatctg cttgaatagc catacaatca
    22501 aaagacaatt tttcaaatgt ctttttcaat ccaaatgctg aagtaccctc taaatcagtc
    22561 ccagataggt cccgtaattt atgaatcggg atatcagttt taaaatattt taaaatcatc
    22621 gaaaggcaag caacaccaca atctttttca tcttgttgct gcacaaaagg aaatgatttt
    22681 atcacttatc taacacaact ccttctaaaa aatttttata gaaaaattct caatatagct
    22741 ttaatacagt gaaaatattg tctcataatt ccacaaaaac tttgaaaaca gttcttgaac
    22801 actaactttt tcattcctga atcaaattta ttttggaaat tttactattc aagaataaac
    22861 ttgataagtt tcaggaagat ttttactgtt aatcaaatac gtatgttaca gtagcaccgt
    22921 aaaaaatatt atttaaagaa gcgtgtttaa acatgcaaaa tgtaaaggaa ctaagtattg
    22981 tggaaatgca caaactgttg gtggaggcaa taatgcttgg ggaaaattgg ggcaagttgt
    23041 aggtggactt accacagggg cagtaggtgg tgcaggttta ggaacagcaa tttgcggacc
    23101 agcttgcggt gttgttggag ggttatatgg agctgtcgct ggaggtgctg cagctggctg
    23161 ggatgctcga aaaaaataaa aaaggagaat aagtagtatg catattaaaa acacaaaaac
    23221 aacatttata ttatcttcag aagagttgaa aaatattcaa ggtgggagtg ccgtgggagt
    23281 tttaggcact actttttctg gggcaacagc aggagtaaaa ctttgttcag ccggaggacc
    23341 ttatgcaata gctgcttgtg gtgtaggtgg agctttatta ggtgctggat tcagcatgtg
    23401 gaccggtgcg taaacaatta aaaaatatca aacctcaaga ttggtttgtg tcgattacga
    23461 acataggtat gttctttatc ttttcaaaag tttggaatgg acagaacttt ttactaaagc
    23521 ttcttgtgct agtttttata attatattaa atttttatct tttattaaag tcagataaaa
    23581 attctttgga aatgtaatgg tagagatatt tttacaaaag gacttgcagc gctgtagttg
    23641 gtgctgcagg aggacctatt tcggccgtta caggaggtat tggtaatgta cttgtcggat
    23701 gtgcattaaa gcctgttcct gcaagttaaa tttataaaaa caggaaagta attatctcaa
    23761 atcctatcaa gtacaatcat aggattgcac ttgacaggac aaatttttta actaaaaaat
    23821 gaaaaacact ttctaaaaat tagtagaatt tgccaaatct ttcgcatctt ccaccataca
    23881 tttaatttta attccccttt atggtactta ataaaaacaa gaaaagaact attcatttgg
    23941 cccttttttg gttccagacc atagaaatat tcctccagta actaatattg ctgttatgca
    24001 cgtaactaat aacagctata aaaattggta caactttaat aggattcaga aaaatttgca
    24061 ctagtgaagg aatctatttt attagaaaaa tgcaatctcc ctaaagaaaa aaccactaaa
    24121 cagtgctttc tttcaacttc tccttaattt tttgtgcttt ccctaaagta aagtttctgg
    24181 ccaactctga atcaaaatat agtgaacgtt ttttaaaatc tatttcttta acgttaaaga
    24241 tatttactaa acacgctcga ctaattcttg ttaacatagg atatttcttt tcaagctcac
    24301 taattcttcc ataaaattcg tactgcccat ctttagtaca caatgataat ctatgtggta
    24361 attttgaagc ttctacaaaa taaatgtcat ttatatcaaa agtaaatgtt tgagaaccta
    24421 tggaaaaaac aaatgcttga ttcgtaacct gttttgttgc atctatcctt tcttgcgcta
    24481 ataacaataa ctcaacaatc tcgtttcgat actcatctag tgtttgatct tttgttacaa
    24541 atcctaacgt ttcaactctt ctttttattg ttacgggtaa catctcatca tgagtagttg
    24601 taaaaatgat ttttgcttga acatcatatt ttctgattac ttctgctaat tctataccgt
    24661 taacttcatg atttaaatca atatctaaga aatatattcc ttgctttggt cggaattgtt
    24721 tgaggtattt tttaacttct aacggacttt gtgttttcaa tactatctta aatactttat
    24781 cgtgaaaaag aatgaaatta tcaataattc gttctaactg atttaattgt ataatttgat
    24841 cttcacataa tattattgga taactcataa atcctccttc cctcaaaaca acaaaataac
    24901 ttgagtagta aattgatacg gatcgttttg atagttcaca aacatattgg gattcttttt
    24961 attgatttcc tctatactag ataaacctaa cccttgatgt cccttttttg ttgttattcc
    25021 ttttttcatt agagtatcta tagagagtag tatctctcta caactattcg atatcgaaat
    25081 ttctatctgt ttatcatctc ggtaaatagc tagagacagt tctctatctt gactctctat
    25141 agatgcttca attgcattat cgagaataat tccaagcacc ctaatacagt caaatattgg
    25201 aacaggaact tcaataatag tacttggaca ttcgaattta caacaaatct tattgttatt
    25261 tgcttggtat agcttcgcta tcaacaaact tttcaaatat tcatttttta tattttttaa
    25321 ataacgatat tcaaacttat tggttagata agaattagaa tagatctcta attcttttat
    25381 ttctttttcc aatatcgaat catggttttg attagcgatt tcttttaaac tcagcaataa
    25441 atttttatag tcatgtctaa atctggcaaa tttttcttga tctttttcca gtgaatcagt
    25501 atattttttt agataaacca attcttttct tttcaattgc tgttcatatt gttctcgttg
    25561 cctaatagtt attctaataa ataaaaatag aacaacaaac atttgaatga ttaaaaatat
    25621 taagacacca aacacaaaat gatcaaatac ttgatattga tgagcaacat aagaaacaaa
    25681 taaaccaatt gtaagtaaat aaatcatgaa tatagcagta agtttagaac tggactgttc
    25741 aacaatccta tttatagcta attttctata taaccaaata aacatcagca aaaacaaaga
    25801 atcaagcatt atctggaata ctacataacc ataccctttt atcccttcaa atgaaaacat
    25861 atacgtcata accatggaag taataataga tctgaaatca tcaataagca tacataatag
    25921 taatttattt aaaagcacta tattagtttt cttcttttcc tttaatataa aataacttcc
    25981 gattataggt atagctgcgg aaacatcagt aaataattca acaaaaaaag tgagtggtat
    26041 caataaacat acaaaaagat attttctttt gttaagatca gtattagtca tataaaataa
    26101 gagaaaaaag aaaaagtttc ccacgaacat tcttaataga taagcttttg tatcgatagt
    26161 aatcaagaga tcccctcctg attttaggat atcataaaaa aaattttagt tacacttttt
    26221 aaataatgaa aaaacacatt ccactaaatt tgatgccggt tttccttgag gttttgttcc
    26281 agcacctcct ttaatttttt taagctcttg atctgaacat tgtttagcat ttagtctatt
    26341 tttttcttcc attacgattc cccctacaaa ctataaatac ttttatgtca ctgattcaat
    26401 atacctattt ttctaatcaa aaaattttga gacatattta atattataag tatcattaaa
    26461 attgagattt atctccataa tctgctcgat ataaaccatt tattccagca gttcttccaa
    26521 tttcactcaa atttcttatt aattcctctt gatattcagt gaatcttatt ttattagtat
    26581 aagcagtaaa atatagataa ttcaccaatc tatttattaa aggaccggga tctaattcat
    26641 atttttgttt ttctaatttt aaatatacct tttgtaaaac ctcaagaata tcagaatatt
    26701 taggatcttt acttatagat atatcattat ataattcatg aacaatttgc ttagcatttt
    26761 ttttcatttt agcacttccc tggaattgct ccacctaaaa aaccacctat agacattcct
    26821 gcaatacaag tagttgcctt ggcccaatcg accgtacatt tatttttagt gcaatacact
    26881 ccatttccat aatattttcc actatgagtg gtacccccat agataagttg tgtctcttta
    26941 atagacaaaa tttttaaatg tttcataatc taatcacccc taaattaatg tttttagact
    27001 attattctaa cattcattaa atttcaaaaa acaattcctt cttgaaacgt agcaaaataa
    27061 ttcctgaata agaatttaat cacttttaag tatataaact atgtatattc tttaatattt
    27121 tttgagaatg cacttcgtac ataataaact ttaatcacat tagtctttca tacctacgat
    27181 cgatctcttt gactcgcacc cataaattca taatagtttg tcggcgtctt tggacgtttc
    27241 gaagatgagc tacgcattct ctttttttat agacaaaaaa gatagagacg aacatggagc
    27301 ttccaaattt ggatatttga tccaattttg gaagctccat ttatttcgtg tctgtctttt
    27361 tattttttcc agctttcgac taattcttgg ttctctttga tccagtcttt tgctgcttgc
    27421 tgtggatctt ttccattgtt gatttccaac ataacttttt ccatatcttt ttctgaccaa
    27481 tggaaattgt ctaatacttt gtaagcttct ggctggtctt ctttcaatcc ttttcgaacc
    27541 atcgtatgga tctgctcttc gcttcccatt ccgttttcag gatcttctaa gtatttcaga
    27601 tcatatttgg caaacatcca atgaggtgtc catcctgtaa ctacaattgg ttcgtgtttt
    27661 ttgatcgctt gacctagtgc aacggtcatt gctcctgaag aagatgtctc tactttccag
    27721 tctttcaagt tgtcatattt ttggatcgtg ttttcagcag ccgtaactac accagctcct
    27781 ggctcgattc ctatgatttt cttacctgcc tgatcggtta actcatcgat cgaatttaca
    27841 tccatgtagg ctggcactgc taaaccaact tttgctcctg tcaaattggc acctaggtct
    27901 tcgacttgat ctttatattg tgcatattgt gaaccatgag ttttaggtag ccaagctgaa
    27961 accatggcat ctgactcgcc atttgaaaca gatttccaca tgattgaatt atctaatggc
    28021 gtcatcgtta catcatagcc catttgtttc aatacttcac cgactacatt tgttgaagca
    28081 acttctgtat cccattcaac ataagataag ttgatacgtt ttgtttcttt ggcagatgaa
    28141 aaactcccta taccaatcgc acctaagatc actactacta cagcaccaat gatcagtcct
    28201 tgacgttttt tacttttctg tgttgttgct cctgcagctt ttttattgtt tggctggttc
    28261 aaatgctgtg taaaacggtc aatgatgatt gctaagataa ctaatgcaac cccgttgaca
    28321 aatccattcc cgacttgtgc tctttgcaat gctgataata cgccgcgtcc taagccgggt
    28381 gcaccgatca tagaggcaat aacgaccatt gaaagtgaca gcattgttgt ttgattgatc
    28441 cctgccatga tcgtactttt agctaaaggc aattctagtt tgaatagttt ttgacgactt
    28501 gtgctcccga atgaatcaga agcttctact aattctttag gaacttgtcg aatccctaaa
    28561 ttcgtaaaac gaacggttgg tggcagagca aagatgactg aggcaaatac ccctggaacc
    28621 atcccgatac cgaaaaatgc aactgctgga atcaaataaa cgaatcctgg cattgtctgc
    28681 ataaagtcaa ggatcggttt gataatactt tgggctttac tgctttttgc catcaagatc
    28741 cctaatggta ccccaatgat gatcgagatc acactagata aaagaacaag tgtgacagta
    28801 ttcatcaaat cattccatag accttggtta taaataaacc atagaccgat taatgtgaac
    28861 aacgtaagtc ctggacgttt cttagaaata aagaaagctg ctaccgtcaa taacaagata
    28921 aacaaaggag ctggaatcac taacagtaaa tttgtaatcc ccgacatcac tgcctgtcca
    28981 atcgtttgta aaaatgaaaa taggccagaa aaagttgtcg taaaccattc tgtgatattt
    29041 tctacccagt ttgccactgg taattgatag ttcaatagat tattcatgtt cactcacctc
    29101 gcttgtttct gctaatgctt caatcacact acctcggatg acgacacctt ttaattttcc
    29161 atcctctaca actgcaagtg gagtcggtga gtcataaata atattgaaaa tatcacttac
    29221 caccatgtct ttcccaatca ccgtcacatt ttcatctagg aagtcgatca atggacgttt
    29281 ttgcttccta gcttctaacg cttgatctgc agtgatgatc cctttcaggt gtctctttct
    29341 gtcaacagcc atcagcatgc tgacttcttc attacgcatc cttgtcaatg caacgtttgg
    29401 tccatcagat tcgatattcg tcgtcaatgc aggtaccatg atgttttgag ccgtaagcac
    29461 tttggaacga tcgacttctt ccacaaattc acgtacataa tcatttgctg gattggtcaa
    29521 tatttcttca cctgttccga tctgcatgat ttcgccgtct ttcatcaagg cgattctgtc
    29581 cccaatccgt aaagcttcat tcaaatcatg ggtaatgaaa ataatcgttt tttgaacatt
    29641 ggcttgcagg tccagtaatt cgtcttgcat ttctcgccgg atcagcggat caagagccga
    29701 gaacgcttcg tccatcagca agatctcagg atcatttgct aaagctctgg ctaggccaac
    29761 ccgttgttgc atcccaccag ataactgact aggatattgg tctttaaatg aaagcaaact
    29821 agaattttct aatgcttttt ctgctttttc ttgtcgttct tctttaggta caccacggac
    29881 ttctaacccg tattctgtat tttccaagat cgttcgatgt ggaaacagac cgaagttttg
    29941 gaaaaccata ttgattttat gacgacggac ttctcgcaat tcttctttgc ttaacttcga
    30001 tacatcttct ccatcaatat agatactgcc cgaagttggc tcaatcagac gattcaatag
    30061 acggatcaat gtggatttcc cactcccgga aagccccatg atcacgaaga tttctccttc
    30121 ttttacgtca aaattcacgt catacacacc aaccgttgca ccggttttct ttaaaatctc
    30181 cgttttcggc tggtgatcat tcatcatatc taaagcggct tgtgtctttt taccaaatat
    30241 ctttgttaaa tgttttactt ctacttttac caaacaaatt ctcctttttt atgtaaattc
    30301 atttttcttt ctgaaaaaag tgaccacact atgttaacat catgtagtca ctttgtcaaa
    30361 aatgaaagaa atttgcttgt ccaaactgaa aaaaacttgg ctaaatcttt gtaaataaaa
    30421 ggtttagcca agttaatatt tttttaatat ttcacacaat cattctgcaa gctgatgctc
    30481 cgggtaaaaa aagtttgtca tacgctgtag tgtatattca tttccaataa aatgaacgat
    30541 gtcccctgcc gtaaatttag cataggggcc aggtgaaaca agcagttctt cattatgaga
    30601 aattgcgata actgtggcgc cagtcgcctg ccagatattc aactcgctaa ttgaacggtc
    30661 cagatgcatt gctttttctg ataaagataa ttcaaacgga agtaaaggat tcatcttatg
    30721 tattcgcttc gtttgattga ccagctgatc caataattga gaaaaatgat tcagctcatc
    30781 ttgttgcttc tccacacttg ccatgatttc tgcttttgta tcttggatgg attgtacatc
    30841 ttgatattgc aataggaaat cttttgcttt ttccttcgaa gcaacaaatg ccccacttcc
    30901 atgccgtact tccatgattc caagatctac cagcacattg atagccttgc gggtagtctc
    30961 aggagaagca ttgtacgtac tggcaagtgt tgagcgggca tggatcttct cacccacttt
    31021 atatcggttt tcaacgatac gttcggctaa atcaacagca atctgctggt agcgcgggcg
    31081 gacgattttt ttctttccag acattttctt tcctctttct ttttttactt tccctcatct
    31141 tactatgctc tagcaaaata agcaaaaaag gggagaatag cctcttgcaa cgactgatca
    31201 tcgcttgatc tgttcctttt ttactgggca agctgtttga cagtcatcac aattcttacc
    31261 ggattttaaa cgagtataga caattgcgcc tgcacttcca aagattaaca cacttaataa
    31321 gaatgtcgcc atcctattcc tctccttcta atgacaatat agtataaaac tggttcttgg
    31381 attttggctg acggaacaaa acataaacga gtcctatcat cagtaaaata gcaagatacg
    31441 tccctaacgc tacctgtcca ccctccaata agtgtcccaa ctgataaatc acaaaactaa
    31501 ctaaataagc taaaccgcat tgatagccta tggcgatcca agtccatttc ttggcattca
    31561 tttcccgatg gatagcccca attgccgcaa aacaaggcgc acaaagtaaa ttgaaggcta
    31621 acaatgaata agctgctgcg ggagtatatg tgtgttgtag cacttgccag atttctctgc
    31681 cattttcgga tacttccgca tgaccaaaca aaataccaaa tgtgccaata atattttctt
    31741 tagcgaccaa tcctgtaatc gctgcgactg ttccttgcca gtttccccaa ccaagcggct
    31801 gaaaaacaac tgccaggatt cgcccgaaaa aggctaagat actttggtct tcagaaacag
    31861 gttgagcata aaaattataa gaagagctga accagatcac gatactagaa acaaagataa
    31921 tcgttcctgc ttttttaaca aaagacttgc tgcggtcata ggtttgtctt aggaccgctg
    31981 accattgagg aaaatgatat gcgggcaatt ccatgataaa aggagctgta tctccagcaa
    32041 acaaacgtgt ttttttcaag ctgattccag agaaaataat tgcagtcata ccaagaaaat
    32101 aggcagaagg agctacccaa gattgatgag gaaaaaaggc ccctgctact aaggcaataa
    32161 tcggcaactt tgcagaacaa ggcatgaagg ttgtgaccat gatcgtcaaa cgacgatctt
    32221 gctcgttttc aatcgtgcga ctggccatga cgcctggcac tccacatcct gtagcaatca
    32281 gcataggaat gaaggatttt cctgaaagac cgaatttcct gaaaagtcga tccatgacaa
    32341 aagcgatcct cgccatatat ccgcagtctt ccagaaagct caagcataaa aacaatataa
    32401 tcaattgcgg tagaaatcct aacactgctc ccacgcctgc gatgatgccg ttcaagatca
    32461 gctcctgcat ccatcccgct acttgccatt cagctaacaa tctgccaaca tgttctggta
    32521 accatgtacc aaacaatgtg tcattgatcc aatctgtccc cattgttcct actgtttgaa
    32581 tagccagata atagatcagc cacatcacaa atgcgaagat cggcaaggct aaccaacgat
    32641 tcgttgcaat ctgatcgatt ttgtcactca tcgataattg gaaagaatca tttgacacaa
    32701 cacagagtgt acatagttgg gtaataaatt cataacgttc attgaccaga atcgtctcac
    32761 tatcgtcgtc caatagtttc tctgttaact ggatcagttg ctcgatctct ttttgctgag
    32821 aaactgctat ctcgtaatcc tctcttacct tctgatctcg ttcaaaatat ttcattgcat
    32881 accagcgttt cttgccgctt tgttctccta gtacatcttc aatttcatgt aaagctgctt
    32941 ctaaacgtgg atcatatgta cagggaacag gaatagaagg ccgctttttt gcagactcct
    33001 gaatcttttc gattagtttt tcgattcctt gctttttcaa tacgctgatt ggtaaaactg
    33061 gtacaccaag accataggaa agcttttcta gattgatttt ttttccttct tttttgatga
    33121 catccatcat attcagacca atgatcatag gaatgtctaa atccaataac tgcgtagtta
    33181 aataaagatt tcgttctaaa ttggtagcat caataatgtt gatgatcagt tcaggctgtt
    33241 cctgaaccaa ataatctctt gcaatcactt cttctggtgt ataaggagac aaagaataga
    33301 tgccgggtag gtcctgaata tgaatgttcg gatttttttt caacgatcct tctttttttt
    33361 ctactgttac gcccggccaa ttccctacat attgattcga accagtcaag agattgaaca
    33421 agctagtttt cccgctgttt ggatttccga ctaacgcgat attcattctt tttcctccct
    33481 ttgtatcaag attagttcgg cctctgactt tcgtaacgtc agttcatacc ctcgcactgt
    33541 cagttcgatt ggatctccca gaggggctag ttttcggata actattttta catttcgcgt
    33601 caatcccata tccattaacc gtcgcttcac tgcgccttgt ccgtggattg ctacgacgat
    33661 cccagtctct cctgctttta attgatcaag agaagtccag ctttcagagg aagcagtctt
    33721 tttattgaca aagatttgac ctagtatttc ccgacttact gccactcgat gactttttac
    33781 cagtaaaatg gcattgttgc ctgataaatt gacgacgacg attttacttc cggcaacgat
    33841 cccgatattt tgcaaatggc gttttgtttt ttcttctgtt tcgattcgtt cgacttgata
    33901 aacgaaattc tgctttgcct gcgctaaggt ctgcatattg attcctctct tgtgaaataa
    33961 atcatgtatt aaaatcattc attatatgtt ctaattttct tacgtgttac gcaaataaat
    34021 gagaatcatt ctcacataga atactctcgg taattgagaa tgtcaattat acttgtgata
    34081 ttatgagcga atctttagta ttgatagata aaaatgagaa aaaaatattt cttcatagtt
    34141 ttccacaatt ttttctctat taaatttgct attttcccac acgatacttg ttttcagctc
    34201 ttttttcaaa aacacaaaac ttagtattcc acagtttcca caacacaata tgtagtgttt
    34261 gttgtttgac ttttttacta agagttttat actatctatt atagataata agaatgtgtt
    34321 agaggaggca ctcaccatga acgtaaaaat cttttctaaa aataattgta tgcaatgtaa
    34381 gatggtcaaa cggtttttaa cagaaaatca aattgctttt gaagaagtca atatcgatga
    34441 acagcctgaa gcagtagact ggttgaaagc acaaggattc caaagtgttc ctgtcattac
    34501 ttctgatgca gaaacagtta tcggattccg tcctgatcag ttgcgtaaac ttgccagcta
    34561 acatgactaa aagaatcaaa acgtgataca tggatttgaa gggcttgaga cagcaccagt
    34621 ttttgtcgca aacttccaaa tcctttttta aagcttaaaa acaatgcaat gatttgcaat
    34681 caagcatcat caaatacgaa atgaaggttg ggcgtacgta tgagtcttaa aaatttacaa
    34741 gatgtcagtt atttcaagct gaataatgaa atcaatcggc cagtcaatgg tcaaatccct
    34801 ttgaataagg ataaagaagc tttagttgcc ttttttgagg aaaatgtcaa accaaatacg
    34861 atgacttttc tatctattat ggaaaaaatc aattacttga tcgatcaaga atatatagaa
    34921 gcagaattca ttcaattgta ttcaccatca tttattgaaa agctatatca atttttggac
    34981 gagcaacatt tttctttcaa atcttttatg gctgcttaca aattttattc tcaatatgcg
    35041 ttgaaaacca acgacggttc cgcttatctg gaaacttatg aagatcgagt agccttcaat
    35101 gcgctttact ttgcgaacgg ggatgaagaa ttagcacttg ctcttgcaga cgaaatgatc
    35161 catcaacgat atcagccagc tacaccttct ttcttgaatg ctggacgtaa acgtcggggc
    35221 gaattggtgt cttgtttctt gactcaagtc actgacgata tgaactctat cgggcgctca
    35281 atcaactctg ctttgcaatt gtcccgtatt ggtggcggcg tagggattac tttatcaaat
    35341 cttcgtgaag ccggcgctcc tatcaaagga tatgagggtg ctgcaagtgg tgtcgttcca
    35401 gtcatgaaat tattcgaaga tagtttcagc tattctaatc aattaggtca gcgtcaagga
    35461 gctggcgtgg tttatttgaa cgttttccat ccagacatta ttgctttcct ttcagcaaaa
    35521 aaagaaaatg cagatgaaaa gattcgcgta aaaactcttt ctcttggcgt gatcgtacct
    35581 gataaatttt atgaattgac acgtaaaaac gaagatatgt atttatttag tccatatagt
    35641 gtcgaaaaag aatatggtgt cccattctct tatgtaaata ttacagctga gtatgatcat
    35701 ctagttgcaa atccaaacat tcgcaaacaa aaaatcaaag cgcgtgatct tgaaaatgaa
    35761 atctcaaaat tacaacaaga atctggctat ccttacgtaa tcaatattga tacagcgaac
    35821 cgcgagaatc caatcgatgg caagatcatc atgagtaatt tatgttcaga gattttacaa
    35881 gttcaaacgc cttctgtgat taacggcaaa caggaatatg aagtattagg aacggatatc
    35941 tcatgtaact taggctcgac aaatatcgtg aatttgatgc aaagccctaa ctttggtcaa
    36001 tctgtgcgta cgatgactcg tgcattgaca tttattacag atgcatctga tatcgatgtc
    36061 gtaccaccga tccaaaacgg aaataagctg aatcatacga ttggattagg tgccatgggc
    36121 ttacacactt atcttgcaaa agaacaaatg gaatatggct cagaggattc attagacttt
    36181 acaaatattt acttcatgct gttgaattat tggacacttg ttgaaagcaa caatatcgct
    36241 cgtgaaagaa aacaagtttt ccataatttt gaaaaatcag cttatgctga cggcacttat
    36301 ttcgaaaaat acgtaactgg agaattccag ccaaaatcag aaaaagtcaa agcattgttt
    36361 gaaggcattt ttatcccaac tgctgaagat tggaatgctt tgaaacaagc agtaatgaaa
    36421 gatggactgt atcatcaaaa tcgcttggca gtagcaccaa atggctcaat ttcatatatc
    36481 aacgatacga gtgcaagtat tcatcctatc actcgcatga tcgaagaacg ccaagagaag
    36541 aaaatcggta aaatttatta cccagcgcct tatctatcca atgagacgat tccgtattac
    36601 acttctgctt atgatatgga catgcgtaaa gtaatcgatg tttatgcaat ggcacaacaa
    36661 cacgtcgatc aaggcatgag tttgacttta ttcatgcgtt ctgaaatccc agaaggcatt
    36721 tatgaatgga agacaacaac gaagcaaacg acacgtgatt tgaatatttt aagacattac
    36781 gctttccaca aaggaatcaa atccatttat tacgtacgta cctttactga tgatgcagaa
    36841 gaaatcggaa gcaatcaatg tgaaagctgc gtgatctaat atcatagata gattcgctta
    36901 ttcatatttt aattaaagga gcattctcat gtcagaaaca tattatgaag caataaactg
    36961 gaatgaaatt gaagatatca ttgataaatc cacttgggag aagctgacag agcaattttg
    37021 gctagataca cgtatcccac tttcaaatga cctagatgat tggcgtacat tgtctcaatt
    37081 ggaaaaagat acagtaggac atgtatttgg cggtttgaca ctgctagata ctgtccaatc
    37141 tgaaagtggc atggatcagt tgcgtaaaga tgtccgcact ccccatgaag aagccgtttt
    37201 gaacaatatt caattcatgg aatccgttca tgccaaaagt tattcttcta tttttagtac
    37261 actgaatacg aaaaaagaaa tcgaagaaat ttttgactgg acaaatacga atccttatct
    37321 gcaaaaaaaa gctgagcgta tcaatgaaat ctacaaacac ggtacaccgc tagaaaagaa
    37381 aattgccagc gtctttttag aaacattctt gttctattca ggtttttata ctcctcttta
    37441 ttatttagga aacaacaaat tggctaacgt agcagagatc atcaaactaa tcatccgaga
    37501 tgaatctgta catggaacct acatcggtta caaattccaa ttaggattca acgagttacc
    37561 tgaagcagaa caagaaacgt taaaagactg gatgtacaat cttctttatg agctttacga
    37621 aaacgaagaa cgttatacag aagaattata cgatccaatc ggctggacag aagaagtcaa
    37681 gacattccta cgctataatg ccaacaaagc attgatgaac ctagggatgg atccactctt
    37741 tccagatact gccaatgatg tcaacccaat cgtaatgaac gggatttcaa caggcacaag
    37801 caaccatgac ttcttctcac aagtcggaaa cggctatttg ttaggtcatg tagaagcgat
    37861 gaaagacgac gattatttga ttggattgga ataaggattt attttaattc aaaaattttt
    37921 cgaaaaataa aaagagttta gcgtacagat attttaaatg tacactgaac tcttttttat
    37981 gcttattcac ttgtttcttc cagcactttt ggactgtcta acttttccga aacatgttcc
    38041 attcttctca ataaatagat tttccggata tttaatatta tcgtcttaat cactgcatag
    38101 acaggtatgc aaatcagcat gcctaatatt cctgcgacac ttcctgctcc aatcaatacc
    38161 aagatgatcg tcaaaggatg gatattcatt gatttcccaa ataacaacgg cttgatcaaa
    38221 tttccatcta attgttggat cactaaaatc gacaacgcca tatacaaagc ttgaaatgga
    38281 gagataaaca aaccgacaat gattgctggt accgctccaa taaaagggcc gacatacggg
    38341 ataatattcg tgattccgca aatgaccgca agcaacaagc tataaggctg atcgaatatt
    38401 ctcattgcca aaaagctcat tacaccaata atcaaagcat ctaagatcgt actgctgata
    38461 taggcagata atgtttcgtt taattctttt accgtttggc gtaactcttt acgaatcgct
    38521 ttcggaaaga agtgagacaa cgcatcaata aatttgtgcc catccttgaa cataaatagc
    38581 aaaataaatg gaacggtaaa taacaaaata aaaaattgca tcataaatga aatgattttt
    38641 gagacacttg cagataaact gactaatatg atattgaaca aattactaag agataaattt
    38701 gctgatgcta attgttcttc caaattgaaa tttttaaact cttcccgctg cgccaattca
    38761 tttaaccatt tgcttgtttc atctgcatac attggcaagc tctgggtcaa ttgaattgtc
    38821 tgttccatta actgaggcac tacattcatg acagttaaaa caactaaaat gattacgacc
    38881 aaaaacgtca ataaaaaacc gaacactctc gggatccgat gcttttcgag aaaaacaacg
    38941 attggatcga acatataata gataaaacca gcaacaagca atggtaaaaa aactgccgaa
    39001 agcattttca atattggact aaaaatattc ggcatttgta aaataaagta cacgccgatg
    39061 atcgtgagta ctagctccac tgtccagaaa aataattttg attgtttaaa acgtgattgc
    39121 atacatctcc tccaaggtcc tttgatactt ctattctatc atttaacaaa gagttacgcg
    39181 ctgagatttt catttgctta aaaaaaacaa aaaaaataaa catattttaa tgagtagacg
    39241 agtacttaaa caaaaacacg gtttggagta gtgacaaaaa ttatactgca gtcccttttt
    39301 ccaaataaac ggtgttcaaa agcttgcatc ttcgtaataa gcccagccaa acgaaaaata
    39361 taaggaacta tttttatttg gctgggctta ttacttgatg taggacatct attttacaac
    39421 ctcagatcat ttattgcgca atactgacta aatgcaactt tgtgatattt ttgtgtacgg
    39481 acaactcctc gatcacatcg gcataagtga gcccttttgg aagatcaatc gtatagatat
    39541 ttttgtacac tcggtagtca tcaacgaatt ttacgtcaaa attcacatct tcgattacga
    39601 tatttttctc ttcaaaataa tcggtaagaa aatctttggt ttcttttcta tggataaatt
    39661 ggatttctaa ctttttagtc gtaggtacat ggatcacgcg tttcactact gtgagtgcaa
    39721 tagcaatccc gataaaactg atgatcgcaa tggaatagta acccatgccg atggcgatcc
    39781 ctaatccagc aattgcccat aaagaagcgg cagtcgtaag tcccgtcacc gattgtttcg
    39841 tgacgatgat cgttcccgcg cctaagaaac cgacaccact caccacttga gcaatcagcc
    39901 ttgcttcatc tgaacgaatc gttccagcaa gctgtggata ctctaaagca tctcttaatg
    39961 caccactagc gatctcaact tggatcaatg caatgataca tgctcccata caaactaaaa
    40021 tatgcgtacg catacctgct ggacgatttt tatattgtcg ttcaaaaccg attgctccgc
    40081 caaatagcat agcgatccct aaacgtaaaa taatctctgg aattgtcaat accatatcat
    40141 tcatctaaac accctccagt ccaatatatc atgtctgaaa ggagcagaca aaaaaagtgc
    40201 aatttttacc tcttacgaaa aaaggactgt gacaaaattt ttgtcatagt cctttttatg
    40261 aataaacggt gttcaatcag ctgaccctcg gtattactca acaaacggca aaatatgggg
    40321 agctattttt cctttgtctt ctctaatact caggtcaaaa cgctgatttc acaacctctt
    40381 tagcctacta ttgaaaatta caacttactt gctgcttctt catcgatgat cacaacaaca
    40441 ttgtcgtgat tttgcaatac acttgctggc aaatgattcg ttacaggtcc ttcaatcatc
    40501 cctttgattg catcagcttt gctttcgcca aatgccatca agacgatttc tttacttttc
    40561 ataattgaac cgattcccat tgaaacagct tttgttggta catcttctgc tttatcgaag
    40621 aatcgtttgt ttgcattgat tgttgattct gttaagtcaa ctactgaagt cgttccgtca
    40681 aatgaagcac caggttcatt gaaaccgata tgtccatttt gcccgattcc taagatttgg
    40741 atatcgattg gatgtgcatc gatgatttga tcatactttt tgcatgctgc ttccagatct
    40801 tctgcttttc catctggtac aaacgtttct ttgaatggtt ttttatcaaa taattggaca
    40861 tccatgaaat gacggtagct ttgttcatct gaaccgccta gtccaacgta ctcatctaag
    40921 tttacagaag tcatttcact gaaatccaaa tcgcttgaag tcatttcttt atataaagta
    40981 actggtgtgc ttcctgttgc caatcctaat acttttgcac ctttgttcat accttctttg
    41041 atgatttcaa atgctttttt tccgccttct tcagcgtttt ttacacgaat gatttccata
    41101 gtgattgctc ctttatattt tggtatagcc caattatagc attctttttt tctttttaca
    41161 ataattagta tagaccaaat tgcatttttt tcattttctt gacaataaat aggaatccgt
    41221 gttaggtttc ataagatggt tcaatcaaac aatccatgtt gcatgaaaac caagcaagtg
    41281 attcactgtg gacgctttat gtctgcagtt tttttatgaa aaaggagtta ttatgagtat
    41341 actgacatta aacaacatta cccagcaatt tggcgacaaa atcctctatg aaggtgtcga
    41401 tctgcaatta aatgctggcg aacatcttgg gctgattgga caaaacggag ccggtaaaag
    41461 tacattgatt aaaatcatca ctggtgagat cttaccagat gacggacaga tccattggca
    41521 aaaaaatatt cacaaaggtt acctcgatca atatgtggaa gtcgatgaaa cattaacaat
    41581 cgaagaattt ctaaaaactg cgtacgcaaa agaatacgaa aaggaagaaa aaattgcttt
    41641 tctttaccaa acatatagcg agaccttcga tgattccttg ctggaaaaag ctgggaaact
    41701 ccaaacggaa ttagaccaag gagtgtttta tcaaatcgac accttagttg ctgagatgag
    41761 cagcgggttg gggatcgatg tcttaggttt agattcactg ttgaaaaatc taagtggtgg
    41821 acaacgttca aaggtgattt tagcaaaact acttttagaa aacccagatg ttctgatctt
    41881 agatgaacca accaatcact tagatgatca gcatatccaa tggctgacgc agtttcttca
    41941 ggattttgac ggtgcgtata tcgtgatttc ccatgaccaa gaatttcttg atcagatcac
    42001 cacccatatc gcagacatcg agtttggaaa gattactaag tatactggcc atctaaaaca
    42061 agcattgaaa caaaaggaac aaaatcgcga aagctatttg cgccaatatc atgctcaaca
    42121 aaaacaaatc gaaaagaccg aagcttatat tcgaaaatac aaagccggtt cccgatcaac
    42181 gatggcaaaa agccgacaaa aacaattaga taaaatcgaa cggctgacgc ctccagcttc
    42241 agcaagtaaa gctgtctttg attttccatt ctcgcctatt gtcacgacat tagcagtgga
    42301 aacaactgat ttagtcatcg gatatgatcg accgctactt gcacccatca atttgaccat
    42361 ccgctttggt gaaaatattg ccattcgagg cttcaacggg attggaaaat ctactttatt
    42421 aaaaacactg atcggtgaga ttgaaaaaat aagtggcgat ttccacttcc cagataacac
    42481 aaaaatcaat tacttttcac aagatctgta ctgggaaaat ccattagaaa caccactaca
    42541 atatttaagc agtcaatttc caaaagcgac tatcaaagaa ttacgtcgcc aactagctaa
    42601 agcagggtta gttaatcaat tagcaagtga acctctcact acattaagtg gtggggaaca
    42661 aactaaggtg aaacttgcac agttgacaat gaaccaaggt aatttactga ttctcgatga
    42721 accgacgaac catatcgatc aagaaacgaa agaaagctta caagacggga tacagaaatt
    42781 tccaggaaca gtaatcatcg tctctcatga acaagaattt tatcaagatc ttgtgcaacg
    42841 agtcattgag attgaaccaa aataaacgtt tgattgaata gttgaaaaaa aggagctgtg
    42901 acaaaagttt tgtcacaact ccttcttctg aataaatggt ggccaattag ctgaccttcg
    42961 gtattagctc aaaaaacggc aaaatatgag gagctatttt tatttggcta ttcttattac
    43021 ttgatgcaga acagcttttc acaacctctt ttcgtctatt ttttattttc gagatttctc
    43081 tttcctttaa aaacaactgt ttgaaagaag aacgcacatc cttgaaaaac aagcccgcta
    43141 accgttacac ctaaccagcc atactgctgc cacattgtcg tccctagcaa tgaaccagcc
    43201 gcacctccaa taaagtacat aaacataaat acagtattat ttcggttgct tgctgcttca
    43261 cctagccctt gtactcgtgt ttgattggcg acttgaccaa attgattccc gacgtctaat
    43321 aaaataatag ctaaaagcaa aatggccaaa tggcttccgc caaccaataa agcaataaag
    43381 cttattgttt gcataacaag tcccatatac acgatttttc tttcagaata agaatctgcc
    43441 agtcgtccca cgataggtgc agcaactgct ccagacaaac caaaaatcgc taatatccct
    43501 gcttcgaatg ttccccaatt gtaggcggga ctgctgatat aaaaaatcaa cgtagaccaa
    43561 aagatcgaaa atgttccaaa catgaaaaat ccactgactg ccgcttctct taatagacgt
    43621 tgagaagcaa ctaaatgcgg caaactttta agtgaatcaa ggtatgaaac agctgtacta
    43681 ctttgctgga cagaacgcgg cattttccag taaagcagaa gtgtaagaat tccgactgca
    43741 cataaagcaa ataaatagat catccgccaa gaaaaaagac tggcaacaat accagatacc
    43801 gtccgagaaa gcaaaatccc cacgagtaaa ccgctaagca atgtacccat gaccttccct
    43861 cttgcttttg gtcccgctag taccgcaccg taaggaatga tgatttgcgg cacgatcgaa
    43921 agtaatccta ttaaaaaaga agcgcatgca aataacgtaa acgaaggcgc aaaaaaagcg
    43981 gccaacagag agacggcaga taacgctgcc attcgaatga tcaattttgg acgatccacc
    44041 acgtccccta aaggaactaa aaacagtaac cctaacgcat agcctaattg ggtcaacatc
    44101 gtcaatatcc cgactgcact tgtgctaaca gataaactag ctgctacttt tgttccaatc
    44161 ggctggatgt aatacatatt ggcaacaaca acgccacaag tgattgataa tagtaaaata
    44221 agcgaagttg ttaaaggttt tttttcatta ctcttcattg atttccccca tttcctatct
    44281 tagtttacaa cttatggaaa agattggaaa tagaaaaata caactattcg ccacacttat
    44341 tatttgtgct tttgttttcc ttgttttttc ttgcgatctt tataaatgaa gacaactaac
    44401 cctactagca cagtaacact tgcaaccaac gtgactgcag ttttgttata tccgtgaaac
    44461 agagcttctc caccacacgc atacaacaca gacgaaggga tgactccaat cacgatcgac
    44521 aatatgattt gctgtagagg caatttcatc gcatctgcag caaaattgac catagaagat
    44581 ggaataaccg gtatcatata gcctaacatt acacctattt ttggatgttt catttgtcgg
    44641 attgcttgca cccaatgatt cgtttcggtt ttcttatcta aaaattttaa gcgatgcata
    44701 aggaaaattg cagtgatgtt accaagagca ttacccaaaa tgttgatgat cgcacctgtc
    44761 agtggtccaa agctcaaacc agcaatgact ccaactactg aagtaggaat acctggaaca
    44821 gcacacatca ccgctgtcaa tacgatcaag agtatcgatg caaaagtccc atgcgaacga
    44881 atatgtgcga gtagctctgc cttactcgct ctcggattta aaaataattg gatgtctgtc
    44941 tgatattcta aatacaaccg atagcccact accagtatca gaatgattcc caacgagatc
    45001 aatatcaatc ggattatttt ctttttcata tttcctcctg aaaaaaatct atattcatta
    45061 aataatagtt taacataaaa acaaaaaagc accgaaatcc aacataggat ctcagtgctt
    45121 ttaaatagta aaaattattt aacagttaca gatgcgccaa cttcttcaag tttagctttc
    45181 aattcttcag cttcttcttt agatacgcct tctttaactg gtgcaggagc gccatcaact
    45241 acagctttag cttctttcaa gcctaaacca gttgcttcac gaactgcttt gataacttta
    45301 actttttggt ctccagctgc agttaattct acagtgaatt ctgtttgttc ttcagcagca
    45361 gcagcgccac cagcagcagc tacagctaca ggagcagcag cagatacgcc aaattcttct
    45421 tcaatagctt taactaggtc gttaagttct aggatagttg cttcttttaa ctcagcaacg
    45481 atgttttcaa tgttcaatgc cattataatt tcctccgttt aattgttctt ttttatttaa
    45541 tgctttatgc ggcaattaag ctgcatctcc atcttctttg tctgccactg ctttgacagc
    45601 gtaagccacg ttgcggactg gcgcttgtag tacagatagc aacatagata gaagtccttc
    45661 gcgatttggc aattttgcca atgctgtgat ttcttccaat gaagagactt taccttcgat
    45721 gataccgcct ttgatttcta atgcttttgc ttctttagca aattcgtcca tgattttcgc
    45781 tggtgcgact acatcttcgt tactgaatgc aacagctgta ggtccagtga atacttcgtc
    45841 catgccttca aggccagctt ttttagctgc acgtgaaaga atagaatttt tgataacttt
    45901 catttctacg ttagcatcac gaagttgttt acgtaaacgt gttacttcat caactgttaa
    45961 tccgcggtag tcaacgacta ctactgatgc agctgctgta aatttttcgc ttacttcatc
    46021 aacgattgct gctttttttg cgataattgc ttcactcatt tttgagtttc acctcctgaa
    46081 ttttgatgga agagtttttt gcactgaaaa ggcaagtcac ttttatagtt actttgctca
    46141 accagtatta ttcatcacct attcacttcg ttcatagtga ttctactcgc tttgctcgaa
    46201 aggaaactcc tttcgctttg ctcaaccagt attgttcatc atctcttcac ttcgttcata
    46261 gtgattcaca ataaaaaact ctatgccacc gcagacatag agggactatt gaatatattc
    46321 aataaacgtc ctcggtagga aattaaggct tgatgccacc tactgtcttc ggtacaagtt
    46381 aattattaac ctcaactagc ttagcatgct aaactagttg agtcaagaat tatttttcag
    46441 ttggatcaat caacattcta atatttaatt agaaagaagc ttggtcaaca tggataccag
    46501 gtccaaatgt tgttgtgatc gttacatttt tcatgtattg acctttagct gctgatggtt
    46561 tagcttttag caatacatca tggattgctg caaagttttc aactaatttt tcatcgtcaa
    46621 atgaaacttt tccgattggt acatggatat ttccagcttt gtccacacgg taagttactt
    46681 taccagcttt cacttcgttt actgcttttg ttacgtccat tgtaactgta ccagttttag
    46741 ggtttggcat taagcctttt ggtcctaata ctcgtcctag acgacctaca gtagccatca
    46801 tatcaggtgt tgcaacaact acatcaaagt caaaccatcc gttttggatt ttttgaacca
    46861 tgtcgtcgtc acctacgaaa tctgcaccag ctgcttcagc ttcttttgct ttgtctcctt
    46921 tagcaaaaac taaaacagtt tgtgttttac cagttccgtt tggtaataca acagcaccac
    46981 ggatttgttg gtctgctttt ttagggtcta cgttcaaacg gtatgcaact tcaactgttg
    47041 catcaaattt tgcaatgttt gtttcttttg ctaaagctac tgcttcggca acaggataaa
    47101 ctttacttga atcaactttt tttaatgcat cttgcatttt tttgctcttt ttagccattt
    47161 tggttcctcc ttgattgtgg ttataacggt tgtacctccc acgtgttcta tatctttcaa
    47221 tatagagcca actgagaagc tgctgtctta gggtgtatag attattctac agtgatcccc
    47281 atgcttcgtg cagttccttc aaccatgcgc attgctgctt caacgttagc tgcgtttagg
    47341 tcttccattt ttaattcagc gatttctctt acttgatcgc tagaaacttt cgcaacttta
    47401 tttttgtttg gttcgcctga acctttttcg attttagctg cttttttcaa tagaacagca
    47461 gctggcggtg tttttgtaac gaatgtgaat gaacggtctt cgtatacaga aataacaacc
    47521 ggaatgatca aacctgcttg atcagctgta cgagcgttga attcttttgt gaatcccatg
    47581 atattgatac ccgcttgacc tagtgcagga cctactggtg gagctggagt tgctttacct
    47641 gcaggaattt gcaatttaac gattttttct actttctttg ccacgagaca tacctcctta
    47701 agtccgtgat gtggttaatt ggagatgcat atttctcctc ccacgtttta tctgatctga
    47761 aaaaatccag acaaataatg tgcaaaacaa tgcacactgg tacagtatag caaatttatt
    47821 tgttttttca agattttttt ctgctatttt tacattttgc ttgaataaac gctaaattta
    47881 ttctaaaaaa acaacaattt ttcgttctca tttcttcttt tatgttacac ttgcgctagc
    47941 atggttcaac agatgaaagc aaacaataac acggtttttt agacccttgt acaaagattc
    48001 ctacataaca aaatgaggtg gagaaaatga aagatttaag gtatagaagt gtctttgata
    48061 ttattggacc tgtgatgatt ggtccaagta gctctcacac tgccggagct gctagaatcg
    48121 ggaaaatcat acgtagtata tttggcgaac agcctgattc tgtagatatt tatctatatg
    48181 aatcatttgc aaaaacctat cgggggcacg gaacagatat tgcattagtt ggcggcctgt
    48241 tagggatgga acccgatgat gaacgtttag ctgattcttt gaaattggca tatgaagccg
    48301 gtatggaagt cttgtttgtt cctaagagtg aaaaagccga acatccaaac tctgtaaaga
    48361 tgcttctaaa aaaaggaaca cgaaagcttt cagtcactgg catttcgatc ggcggtggca
    48421 atatccagat ttctgaattg aatggattca aaatttcttt aagcatgggt actcctacct
    48481 tgatcgttgt ccaccaagat gttccaggta tgattgctaa agtgactagt atactttctg
    48541 agaccaatac gaatatcggc actatgacag tgacacggga atcaaaaggt gaaaatgcca
    48601 ttatgataat tgaagtagat gatcctcaag tagaagaaac ggtaaaaaga ctcaaacagc
    48661 tgccaaatat cgacagcgta aattatttcg attaaggagc taaaaaaatg tttttatcga
    48721 ttgaagaatt agtagatcaa gcaaaagctt atccaagtgt agcagaattg atgattgcag
    48781 ttgaaatgga aatgagtggc cgttcccgta gtcacatcat cgagatcatg gaaaaaaacc
    48841 tctcagtaat ggaacaatca atcaacgagg gaaaagaagg tgttacctcc gtcacaggaa
    48901 ttaccggtgg tgatgcaaaa aaattagatg attatttaaa tgaggcagac tttttaagtg
    48961 gagaaacaat cttaaaagct gtacgcaatg ctgtagctgt taatgaagtc aatgcaaaaa
    49021 tggggttgat ttgtgctaca ccgactgccg gaagcgcggg tgttgttcct ggtgtgctga
    49081 tggctgtccg agatcggctt gatttgacac atgaacagca attggaattc ttatttaccg
    49141 ctggcgcttt cgggttagtc atcgcaaaca acgcttctat cagtggtgca gaagggggat
    49201 gtcaagcaga agttggttct gcaagtgcaa tggcagcagc agctttggtt tgtgcaaaaa
    49261 atgggactcc tcatcaagca gcacaagcag tagccattac attgaaaaat atgatgggac
    49321 tgatttgcga tcccgttgca ggcttagttg aagtcccgtg tgtcaaacgt aatgctttag
    49381 gctcttctca agcttttatc tctgctgata tggcattagc gggtatcgaa agtgtcattc
    49441 cgccggatga agtcgttgct gcaatgtacc aagtcggacg tcaaatgccg tcgattttca
    49501 aagaaacagc agaaggtggc ttagctgtta cgccaactgc taaacgactt gcggaagaga
    49561 ttttaaataa gagggcgtga taaagctcct taatttcgag tattaagaga ttagttatct
    49621 tcataacaaa aaacgaagag aggactaatt gcccttctct tcgtttttta ttaatccatc
    49681 aatttcgtat tttttacttc atcgatcgtt tttgttcctg caagttgcat cgtgatcgac
    49741 aattctttgt tcaggtgttc aaaaacagat gttactcctt ctgccccacc tagattcaac
    49801 ccataaatca ctggtcggcc aatcgcaact aagtcagcac cacttgccag agctttgaag
    49861 acatgctctc ctcgacggac accagagtcg aaaatcactg gtacacgttt gttgactact
    49921 tcggcaattt ttggcaatac ttcaaaggac gcaggacctc catctagttg acgtccacca
    49981 tgattggata cccagatacc atctgcccct gcagagatag cgattactgc atcttctggc
    50041 gattgtattc cttttacgat aactggaaga tgggtgattt ctttgattgt tttgatgtca
    50101 tctggtgtca agccttgttt tgcagctgca taaatttctg cgattccttt tccttcgcca
    50161 ttaccacttg cactttgttc gctatacgca gctaagttag gcattggtaa tggaaattgg
    50221 aattgattga tgacgtcttc ttcacggtaa ccaccgagcg ttgaatctgc cgttaaaatg
    50281 atagctttag cccctgcttc tactgctttg ttcaaaatga actcattgaa tctgtcgtct
    50341 ttactcatat ataattggaa aaactgcggt gcgcctggcg ctgcatcggc tgcgtctttg
    50401 attgtcgtat ttgcataagt gctgatggaa aagattgatc cagcagcagc tactccttta
    50461 gctgtatcag cttccccttt ttcatgtgcc aatccttggg ccgctgatgg cgcttggata
    50521 atcggtgtat ctaattcaat tccgaaaaca gatgtatgca aatcggctga gtcgattccg
    50581 cgtaatatcc gtggcatgat tttctttgta ttaaaagaag tcgtattttc tttcatcgtc
    50641 cactcatctt cagaaccacc gcgaatatag ccaaaagctc ctgcttccat gcgctctttt
    50701 acacgacctt ctagttaggc aatgtttaca atatcgattg gatgttcttc attacttgta
    50761 tgataagtca tagtagatcc ttctttcatc ttcttgatta catataaatc atactaaact
    50821 tacaaaaagt aagcaaacat ttactctcag atacttttat cgctattctt tccaaataaa
    50881 tcgacttaca agctgatcta cttcttggtt ttcatgaagc atactatgag aggcattttc
    50941 acctgtcact actttttcct ctatttcatt tccgcgttgc ttcaacaacg catagaccgc
    51001 taatgcactg tttaacggta ctgttccatc actcaagtct gtttcatcta gttgaccagc
    51061 aatcaagaaa aatcgagtgg ttataggaat attaccgatc atttgctggt aatcttgata
    51121 gcggttagaa acgacagcag gcccctttgc taactcatct gtcagagact gcgcactatc
    51181 atcgacaaaa tcattaaaag gcgcgccaat tgctgttaat gtctgaactt gtggcaatga
    51241 cgtatcctgt ccatacgttc ctaaatagcg tagtgcacta actcctccca tggaatgccc
    51301 cactatgttg actttatcta tgccgtagtt ttctttcaga tacaaaagac aagttttgat
    51361 ccattctgct tgattccact cgttattttt attatcttca aacaatactt ggaccatcgg
    51421 attgtctgct tttccagaca aatctcctgt tgcttgtatt tgtccatctg ttcctactgt
    51481 taaaaccaat tcttttactg cttgctgatt tttttccaaa cgccgcaaca tccctttaaa
    51541 ggaattttct gtcccgctgt agccgtggaa aaaaagtgtt ggaatagtgg ttttttctat
    51601 cactttactg gattctgccg ttttttcttt atcagaaact tcagtagtat ttatcttcgt
    51661 cgtctggcaa ccacttaacg caaacaaact gataaggact aaagctgctg cagttgcacg
    51721 tttgcataaa tttttgattg gttctgtcat acacctcttc ctttccatat agaaaaaaga
    51781 aagacttgaa aggccaatca tatgatcgac atttcaagtc tctttattaa tcgattttat
    51841 ctacttgatc gaaatcaagt tctgtactgg tttcacgtcc aaacatatcg atgttgactt
    51901 tcaatttttc tttttctccg tcaacttctg ttacttgtcc ttctaatcca gcaaatgcgc
    51961 cttcgatgat tttcacgact tcgccaactt ctacttctag atcattttgt cgcaggctca
    52021 taccgataga acgcaagata tgattgactt cttctggaag taatggtgct ggtttgcttc
    52081 cggctccgtg tgaaccaacg aatcctgtca caccaggtgt gttgcggaca acataccatg
    52141 aagcgtctgt catgatcatt tcaacaagta cgtatcctgg gaatgtttta tgaacgattt
    52201 ctttttcttt cccatttttg acttcagttt ctttttcttc aggaacaacg acacggaaaa
    52261 taaagtcggc cattttcata ctttgcgcac gagattcgat atttgctttt actttgtttt
    52321 catatcctga atatgtatgc aagacatacc agtttttttc aaaagattcc attgaaggct
    52381 cctcatttca ttgtaaaata aaaaaacctc agtacacccg aagtttttct ttcgcttcat
    52441 tatagcacta aaagtacgtt gtggctagcg agttatttta aaatccagcc aaataagctt
    52501 tggatggctg tatccattac gtaaaacatc aatccgaaaa taatagaagt ttcgatgact
    52561 actaacgtat ctttacggag ttgttttctg cttggccaag ttacttgttt gatttcatct
    52621 tttacgcttt ttagaaattt catgagacaa cctcccttgc tactgtttat ttcgtttctt
    52681 tatgtaatgt atattgatta caatatttac aaaacttatt gatttccaaa cgttcgccgc
    52741 tttttccttc actgactgat tttgtgtagt tgcgagaccc acaaacggaa caagcgagtg
    52801 ctgctttttt ctttgccatt tacttctgcc cccttgctga gaatcagact tatacttcat
    52861 aacagtatca cgtaactttt tacttgtcaa tttgtgaaat aaactatcta ttgttttgga
    52921 taaaattatg atatgattaa tttgaaatta agctaaaatg agacaattgg aggtatagcg
    52981 atgctattaa aaacacttgt atataagaaa aaagatctga caaccgttac agagactagt
    53041 acattagaag aagcattaaa aattttagaa acttccggat accgttgtgt gccgatctta
    53101 gattcttcag gtaatatttt ccgtggaaac atttacaaaa tgcatatcta tcgtcataaa
    53161 gcaaatggcg gtgatatgac gcttcctgtc actcatcttt tgaaaaacgc tacaaagttc
    53221 atttacctgg atacttcttt cttcaaagta ttctttacga tcaaagagct tccttatatt
    53281 gctgtccttg acagtgaaaa tcatttttat ggtattttga cacatagtac gttgcttaat
    53341 atgctctctc aatcttggag cgtagaacaa ggaagctatg tactgacaat tgcttccaca
    53401 ggaaagcaag gagatttggc tgctattgca aaaatcattt caaaacacag cagcattgcc
    53461 agctgtatta ctcttgatat cgaaaaagac gaatacattc gacgtacatt gatcacgcta
    53521 ccggctaaca ctgaagcgaa agtatgtgaa acgatcgtca accacttaga gaaaaagaat
    53581 tataaagtgg tagaaattga aaatttgcaa aaccaagaag cataatcaaa aagcgaaaaa
    53641 agaaaacagc taatctgctt tcttttttcg ctttttataa ttagacacgc ttttcaaaat
    53701 caagcgcgcg atctaaataa tacatcaatg gtgctgaaac agccatgacg ataaactcac
    53761 ttaatgcaag cgtgccatag gttgtccaaa atgcttggcc acctgttgga actaacatat
    53821 aagcaatcag tcccatgctg atggtaaaga agatcgtgtt caatactaaa cgcatcttta
    53881 catttttcac tttgttttgc aataaagctg tcaggcctaa agaaagcaat gtttggccgc
    53941 caccaaaaac cacatccatg ataccaaaac caaagaaggc attatagacg atgaccccgc
    54001 ctaaaactcc ccaaagcaat ttacggttaa agacaactaa atgattcaaa ctttctgaaa
    54061 tacgaaactg gatcggccct gaggatacag gcgcaactaa aatagttaac gctagataaa
    54121 gagccatgat gatgccattt acaacgatta cggataaccg ttgtctgctt ttcgtttttt
    54181 gcattttctc tcctcttttc tcctagtttt tttaacgcgg gatggttgat gaaccgcggt
    54241 tatgtgaaac aaacaataat tataacagac ttttcattta tttcaatccg tcttatctgc
    54301 ttttaaaaaa gcaaaagctt aaacaatgta aacgtttgct aaaaatcgtg ggatatggta
    54361 taaagtaagt gtaaggggtc ataagaattc ccagtaaagc agacgaagta ggatctgcaa
    54421 gaacagaatt gttgtgaaac ttaaataaat aaaaaaggat gtgggaattt gagcaacctc
    54481 ttactagaga acggtctagc aagcagctga agatctaagt ggacatgccg aaccttggaa
    54541 cttctcctta cgtaagtaat ggatcatgcg cttgctaggc ttttttgtgt ttttcggtct
    54601 tcggaccttt ttcattttcc tagacttctg ctataataac caagaattaa ttttgtaaca
    54661 gatttttttg aaaaaacaag tttctttcat tcaaaaaata gaaaaagctt gctaattatt
    54721 ggttaaccag caaacaggag tattaaaata tcttgtttta ttgaatagca ggaggaaatc
    54781 atgaaattcc attatcatat attgatcaat ccatcggcag gcagcggaaa tggctataaa
    54841 gtagcagaaa gaattttacc ggtattaaaa aataagcata ttgactatac acttcattat
    54901 agtgaataca aaggacacga tgctgagatt gctgaaactt tggcaaaaga aacattgatt
    54961 ccttggatag aagaagaaca gaaaacaatc gatactttcc cccttttgat catcgtagga
    55021 ggtgacggca ccttacatca ggtacttgat actttctatc agatggaagt agaattccca
    55081 gttgcttata ttccagcagg ttctggagat gattttgctc gaggggcagg ccttccaaaa
    55141 aatcccaaaa aaggacttca gctgatttta gcagctcaat caccagaaaa agtccacata
    55201 atggcttatg aagaaaaaat aagtgaaaaa aaaggaatag ctgttaataa tttcggtata
    55261 ggattagatg cagctatcgt ccatgcgaca aatcattctt ccacaaaaaa gcgattgaac
    55321 aaatacaact taggatcatt ttcgtattta ttctctattc tccgtgcttt attcactcaa
    55381 aaaggatttc caattctggt agatgcagga ggaaaacaat taagtttttc aaacgcattt
    55441 ttatgtacag caacgaagca tccttttttt ggcggaggaa tcgcgattgt tccaactgct
    55501 gatgcgtcta aaccagtcat tgattttgtc gtggtcgaac gaatcaattt ctttaaaatc
    55561 ttgtggctca ttcttttact tatacagaaa aaacaattga aatcgaaata tttccatcac
    55621 tacacaacaa acagattacg tatcgtttca acaacaccgc aacatggtca agaagatgga
    55681 gaagaaatgg aaaaacgacc ttttgatatc acaataacca caactaaaca gttgttttgg
    55741 tgttaataaa agaggttttg tcagaagcta catctggaac accgtttatt cggaaaaaag
    55801 gactgtgaca cgacttttgt cacagtcctt tttttactat gcagttaatt ctttgcccaa
    55861 ttgggttgcc atcgatagtt tctttcttta cccaaaataa attggatcat tgaaaacacc
    55921 gcagcaaaag caactaagac acaaccattg aaccaaaaag attctgcaaa tgtttgattg
    55981 taactgagtg tttcactgat caagttatta ttttgaacaa accaatatgt tggtgtgaat
    56041 gccgctattt tgctaaccat ttctggcaag tattctgctg gtacaaacac cccaccgata
    56101 aagcaactgc ctgtgataaa aatatctttg atcccaccgc tggtttctga gttttttacc
    56161 aatgaagtga tacaagccga aaagctaatg atcggcaata aaaacaagaa agtatttaaa
    56221 atatgatacc ataccgttat atcccacgag gtgtggatca ctaacagaac cattagaagt
    56281 aagaagccac tgaacaacag cagcgagtaa ctaatcaaac taaaggttac tttgcgattc
    56341 aagtttcgtt tggtaatggg cgcactctgg tttcgtttac tgatctcttt gcgattaaat
    56401 gctagattga tgacgctgaa accgctaaaa atggatagaa acaacccata agacaagaaa
    56461 ttaaaggttg tgcccgtcaa cagctttttc atccgctgac tatatcccgc acttagctca
    56521 acttctccag taaccgagag tgccatttgt gtctgttcca tagctgcatc gattgtttta
    56581 tcttcttctt gaaacagaat cagtgtttgc aaaaaggtgt tcacttgctg tgtaacaact
    56641 gttttagaaa aggcatctgg gcgactctgt atcttgatcg tcggcatctt tcctacttcg
    56701 acttgttcgg taaagtctgc aggcagatgt aagataatgt ttacacgatt aaaataaaga
    56761 gcatcatcga tttctttttg tgaggttcca tccaatgtaa cgactgtttc cttgtcattc
    56821 aaatatttac gaaggccatc gactacaggc gactcttctt cactaatcaa ggcgattttc
    56881 gcatcttgca gagaggtatt ttcaacatca atattttgcg caaaaaagaa tgtggcaaca
    56941 acagtgataa ttacacctat cagtaaattc gatttatttg ctttaagcaa tgtccaaatc
    57001 gttttataaa gcgtcataac gcacccctct ttccatccaa gcatttccta acgcaaaaag
    57061 gaaaataaat ccgatcaacc atagaagatt ggtataaaaa ctagccaaat cagtgtagaa
    57121 ataaagttt
//
LOCUS       DACDXO010000010        55940 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E23605_2017
            SAMN12639989-rid9881463.denovo.010, whole genome shotgun sequence.
ACCESSION   DACDXO010000010 DACDXO010000000
VERSION     DACDXO010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639989
            Sequence Read Archive: SRR10023389
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 55940)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55940)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741914.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:42:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,972
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 33 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 17 of 73
            Pseudo Genes (multiple problems)  :: 10 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55940
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639989-rid9881463.denovo.010"
                     /isolate="E23605_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            242..1456
                     /locus_tag="IWM35_000751"
     CDS             242..1456
                     /locus_tag="IWM35_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HAP9918625.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
     gene            1487..2914
                     /locus_tag="IWM35_000752"
     CDS             1487..2914
                     /locus_tag="IWM35_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP9918626.1"
                     /translation="MQQSKTKIPAKKLTFFGFLAMTISMVVSLYEYPTFATSGFSLVF
                     FLLLGGLLWFIPVALCAAEMATVKGWEKGGVYTWVSRTLGKRFGFAAIFFQWFEITVG
                     YLTMLYFLTGALSYATGIGAIQNNKYLKLAILLIIFWAILLSQLKGTKYTSLIARIGF
                     VAGILLPALVLFGLGIHYVASGAPLQTILSMKTLIPDFSKLPTLVVFVSFILAYMGVE
                     TSASHANEMENPKKDYPLAMFVLVILAIILDTFGGLTVATTVPQHGLSLNTGVIQSLE
                     FLLRHIDPNLAWIAKILAILVCLGVIGEIASWVTSPSKALHVAAEEGLLPEYFAKENT
                     HGVPAHLMIANGIVATIWAAVFTLSGGGNNLSFLAAMSLTVVIYLMLYFMFFAGYLKL
                     VFKDDSLERAYQVPGGKVVKVIVACLGLLTSLFAFCISFVPPASIAAGQHGIYETILI
                     VSYLIVLVIPFTIYACRHKFQHAEK"
     gene            2937..3863
                     /gene="glsA"
                     /locus_tag="IWM35_000753"
     CDS             2937..3863
                     /gene="glsA"
                     /locus_tag="IWM35_000753"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HAP9918627.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            4071..5531
                     /locus_tag="IWM35_000754"
     CDS             4071..5531
                     /locus_tag="IWM35_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9918628.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTELADLEFISLSKVYTIKKIVLDFFKENDYLKNKEIIIPEFDSRNILLALVRYVNW
                     EGYENKNPYVEEACNELIDYVETHFFKRRYSEDEKSFIIRGIEIAIGRKAFPLGLKEE
                     DKKSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEV
                     VYKNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYL
                     LSEKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFV
                     VSESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQN
                     GLETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            5752..7515
                     /locus_tag="IWM35_000755"
     CDS             5752..7515
                     /locus_tag="IWM35_000755"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HAP9918629.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            7599..8057
                     /locus_tag="IWM35_000756"
     CDS             7599..8057
                     /locus_tag="IWM35_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HAP9918630.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            8166..8849
                     /locus_tag="IWM35_000757"
     CDS             8166..8849
                     /locus_tag="IWM35_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HAP9918631.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            8996..9610
                     /locus_tag="IWM35_000758"
     CDS             8996..9610
                     /locus_tag="IWM35_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HAP9918632.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            9626..9832
                     /locus_tag="IWM35_000759"
     CDS             9626..9832
                     /locus_tag="IWM35_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9918633.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGLFNYFNK"
     gene            10371..10928
                     /locus_tag="IWM35_000760"
                     /pseudo
     CDS             10371..10928
                     /locus_tag="IWM35_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303250.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
     gene            10986..13025
                     /locus_tag="IWM35_000761"
     CDS             10986..13025
                     /locus_tag="IWM35_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HAP9918634.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDKYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGGKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKIAFDSLGGKAASTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKMITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSFKTTRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLITPIQQAAMYSVFTNNGTLVY
                     PKLIADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLFAFNLDNQGYMMVSMLENKEDDDSATKRASELLQYLNQNYQ"
     gene            complement(13134..14285)
                     /locus_tag="IWM35_000762"
     CDS             complement(13134..14285)
                     /locus_tag="IWM35_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HAP9918635.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVILLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
     gene            complement(14439..14969)
                     /locus_tag="IWM35_000763"
     CDS             complement(14439..14969)
                     /locus_tag="IWM35_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9918636.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            complement(15068..15418)
                     /locus_tag="IWM35_000764"
     CDS             complement(15068..15418)
                     /locus_tag="IWM35_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HAP9918637.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
     gene            complement(15575..17104)
                     /gene="ahpF"
                     /locus_tag="IWM35_000765"
     CDS             complement(15575..17104)
                     /gene="ahpF"
                     /locus_tag="IWM35_000765"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HAP9918638.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     RTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            complement(17114..17677)
                     /gene="ahpC"
                     /locus_tag="IWM35_000766"
     CDS             complement(17114..17677)
                     /gene="ahpC"
                     /locus_tag="IWM35_000766"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003756453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HAP9918639.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            complement(17821..18483)
                     /locus_tag="IWM35_000767"
     CDS             complement(17821..18483)
                     /locus_tag="IWM35_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP9918640.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            18667..20313
                     /locus_tag="IWM35_000768"
     CDS             18667..20313
                     /locus_tag="IWM35_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HAP9918641.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAVLLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPNMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDLQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            20304..21386
                     /gene="uxuA"
                     /locus_tag="IWM35_000769"
     CDS             20304..21386
                     /gene="uxuA"
                     /locus_tag="IWM35_000769"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAP9918642.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFGLPGWEP
                     ERLAPIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLA
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNGYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
     gene            complement(21494..23047)
                     /locus_tag="IWM35_000770"
                     /pseudo
     CDS             complement(21494..23047)
                     /locus_tag="IWM35_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
     gene            23195..24336
                     /locus_tag="IWM35_000771"
                     /pseudo
     CDS             23195..24336
                     /locus_tag="IWM35_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
     gene            complement(24462..25541)
                     /locus_tag="IWM35_000772"
     CDS             complement(24462..25541)
                     /locus_tag="IWM35_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP9918643.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVIDFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVVALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(25650..26090)
                     /locus_tag="IWM35_000773"
     CDS             complement(25650..26090)
                     /locus_tag="IWM35_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP9918644.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTSLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTPQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(26147..26557)
                     /locus_tag="IWM35_000774"
     CDS             complement(26147..26557)
                     /locus_tag="IWM35_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HAP9918645.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(26738..27172)
                     /locus_tag="IWM35_000775"
     CDS             complement(26738..27172)
                     /locus_tag="IWM35_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAP9918646.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVSFEERKQLFEKYM"
     gene            complement(27272..28120)
                     /locus_tag="IWM35_000776"
     CDS             complement(27272..28120)
                     /locus_tag="IWM35_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP9918647.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAVLGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLFFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFVFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
     gene            28509..30275
                     /locus_tag="IWM35_000777"
     CDS             28509..30275
                     /locus_tag="IWM35_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HAP9918648.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETRRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVRDHLDYLTDLGVNGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVYDREDKLYFTRELEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            30377..31636
                     /locus_tag="IWM35_000778"
     CDS             30377..31636
                     /locus_tag="IWM35_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP9918649.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            31783..33084
                     /locus_tag="IWM35_000779"
     CDS             31783..33084
                     /locus_tag="IWM35_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP9918650.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            33087..33938
                     /locus_tag="IWM35_000780"
     CDS             33087..33938
                     /locus_tag="IWM35_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP9918651.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            33951..34766
                     /locus_tag="IWM35_000781"
     CDS             33951..34766
                     /locus_tag="IWM35_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HAP9918652.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            34917..36083
                     /locus_tag="IWM35_000782"
     CDS             34917..36083
                     /locus_tag="IWM35_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP9918653.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
     gene            36313..36960
                     /locus_tag="IWM35_000783"
     CDS             36313..36960
                     /locus_tag="IWM35_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HAP9918654.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            37044..37988
                     /locus_tag="IWM35_000784"
     CDS             37044..37988
                     /locus_tag="IWM35_000784"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HAP9918655.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            38123..39574
                     /locus_tag="IWM35_000785"
     CDS             38123..39574
                     /locus_tag="IWM35_000785"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HAP9918656.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            39595..40431
                     /locus_tag="IWM35_000786"
     CDS             39595..40431
                     /locus_tag="IWM35_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HAP9918657.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            40431..41090
                     /locus_tag="IWM35_000787"
     CDS             40431..41090
                     /locus_tag="IWM35_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAP9918658.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSIHQS
                     I"
     gene            41216..41905
                     /locus_tag="IWM35_000788"
     CDS             41216..41905
                     /locus_tag="IWM35_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9918659.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PKDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            41911..42867
                     /locus_tag="IWM35_000789"
     CDS             41911..42867
                     /locus_tag="IWM35_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HAP9918660.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHPIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            42911..43423
                     /locus_tag="IWM35_000790"
     CDS             42911..43423
                     /locus_tag="IWM35_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9918661.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSGVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(43679..44227)
                     /locus_tag="IWM35_000791"
     CDS             complement(43679..44227)
                     /locus_tag="IWM35_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP9918662.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(44316..45497)
                     /locus_tag="IWM35_000792"
     CDS             complement(44316..45497)
                     /locus_tag="IWM35_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HAP9918663.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
     gene            complement(45733..47241)
                     /locus_tag="IWM35_000793"
     CDS             complement(45733..47241)
                     /locus_tag="IWM35_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HAP9918664.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATCLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            47400..47591
                     /locus_tag="IWM35_000794"
     CDS             47400..47591
                     /locus_tag="IWM35_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9918665.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            47794..48711
                     /locus_tag="IWM35_000795"
     CDS             47794..48711
                     /locus_tag="IWM35_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="viral A-type inclusion protein"
                     /protein_id="HAP9918666.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            48704..49777
                     /locus_tag="IWM35_000796"
     CDS             48704..49777
                     /locus_tag="IWM35_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9918667.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            49925..50443
                     /locus_tag="IWM35_000797"
     CDS             49925..50443
                     /locus_tag="IWM35_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9918668.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            50424..50816
                     /locus_tag="IWM35_000798"
     CDS             50424..50816
                     /locus_tag="IWM35_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HAP9918669.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVVFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            50892..51827
                     /locus_tag="IWM35_000799"
     CDS             50892..51827
                     /locus_tag="IWM35_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HAP9918670.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            51960..52739
                     /locus_tag="IWM35_000800"
     CDS             51960..52739
                     /locus_tag="IWM35_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HAP9918671.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            52732..53049
                     /locus_tag="IWM35_000801"
     CDS             52732..53049
                     /locus_tag="IWM35_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HAP9918672.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            53293..54903
                     /locus_tag="IWM35_000802"
     CDS             53293..54903
                     /locus_tag="IWM35_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAP9918673.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            55060..55908
                     /locus_tag="IWM35_000803"
     CDS             55060..55908
                     /locus_tag="IWM35_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAP9918674.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
BASE COUNT        18550 a         9924 c        10899 g        16567 t
ORIGIN      
        1 tttattcctt atgaaaaaat atctttaaaa ataaaaaaag acgaaaataa acggttttct
       61 ataaaaagaa gaccgtttat tttttgtctt tatttgtgtg aaaaacgaaa aagagcacaa
      121 cacatcaagc ttttattaat tgtctgtctt ttataaatgc aagtattaat tcaaaaaaac
      181 agaagaaaaa aacaactaca ccttctttta tactcgtaat cgtagaaagg aggcgaaaaa
      241 aatgaataca ttacctatca atacaggaga tacagcattc atgattttat gtacagcaat
      301 ggtttgcctg atgactcctg gtctagcttt tttttacggc ggattagcac ggaagaataa
      361 tatactaacg atcatgtctc aatcttttct ctccgttggg ataacaacga ttatgtgggt
      421 atttggtgga ttcggattgg cctttggacg ggatatcggc ggtgttattg gaaacccggc
      481 agatttcttt ttgatgagac atgttacaag cttccccaac atgtttcatg gtgcaacgat
      541 tccattttta atgttctttc tttaccaact gatgtttatc gtgattaccg ttcctttgat
      601 gacaggagct tttgcaggga gattgaattt aaaaggctat attatacttg ttattttttg
      661 gaatctgctg atttatttcc ctgtttgcca ctggatatgg ggtggcggat ttttggatca
      721 gatgggattt agagatttcg ctggtggagc agtcatccac acgacagctg gattcggctc
      781 tttagcatgt atcctcacac ttggtcaaag aaagattcct ttaggagaaa gaagtcagca
      841 tagcagtctt atggcagctg ctatcggtac aggactgctt tggtttggct ggttcggttt
      901 taattccggt ggtgcacttc gagcagatga acaagcagtc aatgcatttg gttcaacatt
      961 catcgctctt gcatttgcga tgattacttg gctgatcatt gcaaaagtaa aaggaaatgg
     1021 ctttgatttt gtcgacgtac taacaggatc cgttgctgga ttggcagcca ttacgccttg
     1081 tgccggttat gtagaagcaa aaagtgccat gctgattggg atcattgcgg gtatcgtctg
     1141 tcatgctgct gttgattttc gtaaaaagaa acaatgggat gatgcacttg atgtgtgggg
     1201 tgttcatggc atgggcggat tcacaggtac gattctgata gggatctttg catctaacag
     1261 ccatttacta acgaatccaa gcagctggta cttcttaggg attcaagtga ttggtgtcat
     1321 cattactgcc gtgtatgcct atgtgctgac aacagtgatt ttaaaatcgg cgatgcactt
     1381 tacaacgatc actactacaa aagaagaaca agaagaagga ttggactttt tcttctttgg
     1441 caacaaactc aattgaaaca aattcaaata gaaaagagga agattcatgc aacaaagcaa
     1501 aacgaaaatt cctgcaaaaa aattaacgtt ctttggcttt ttggctatga ccatctcaat
     1561 ggtcgtttca ttatatgaat atcccacatt cgcgacctca ggcttcagct tagtattttt
     1621 cttgttatta ggcggtttgc tatggtttat ccctgtggca ttgtgcgcag cagagatggc
     1681 aacggtgaaa ggatgggaaa aaggcggtgt ctatacttgg gtcagtcgca cattgggaaa
     1741 acgattcggt tttgccgcaa tctttttcca atggtttgag ataacagttg gctacttgac
     1801 catgctgtat ttcttaactg gtgcattatc ctatgcaacg ggtattggtg ctatacaaaa
     1861 caataaatat ctgaaattgg caatcctatt gattattttc tgggctattt tactctcaca
     1921 gttaaaagga accaaataca cttcattgat tgctcggatc ggttttgtag ctggtatctt
     1981 gttgccagca ttagtcttgt ttggcttagg gatccattat gtcgcttcag gcgctccact
     2041 acaaacgata ctgtctatga aaacattgat tcctgatttt tcgaaattgc ctacgctggt
     2101 tgtttttgtt tcctttattt tagcatatat gggcgtagaa acatctgcca gtcatgcaaa
     2161 tgaaatggaa aaccctaaaa aggattatcc attagcaatg tttgtattgg ttattctggc
     2221 aatcatttta gatacttttg gcggattgac cgtcgctact acagtacctc aacatggttt
     2281 gtctcttaat acaggggtga ttcaatcgtt agaattccta ttaagacaca ttgatccaaa
     2341 tcttgcatgg attgcgaaaa ttcttgcgat tcttgtttgt ttaggtgtca tcggagaaat
     2401 tgcttcttgg gtaacctctc cttcaaaagc acttcacgta gcagcagaag aaggattact
     2461 tcctgaatat tttgcaaaag aaaacacaca cggtgttcct gctcatttga tgatcgcaaa
     2521 tgggatcgtt gcaacaatct gggcagccgt ctttacatta agcggcgggg ggaataatct
     2581 ttcatttctt gcagcgatgt cattgactgt tgtaatctac ctgatgttgt actttatgtt
     2641 tttcgcgggc tatttgaagc tggtttttaa agatgacagt cttgaaagag cctatcaggt
     2701 tccaggcgga aaagttgtaa aagtaatcgt tgcttgttta ggtcttttga cttcactttt
     2761 tgctttttgt atctcatttg ttccacctgc tagtatcgca gctggacagc atgggattta
     2821 tgaaacgatt ctgattgttt cttatcttat cgtcttagtt attccattta cgatctacgc
     2881 atgccgtcat aaattccagc atgcagaaaa ataaaaaatg aaagctggtt attcaaatgg
     2941 aagtaaaaga attagaacaa ttgattggaa aaaatttcca ctattatcaa acaggacaag
     3001 tggcggatta cattccagca ttagctgaag tggatcccaa acaattaggt atggcgattt
     3061 atgatttgga tacaaaacaa ctgattgagg gaggagacgc cgatgtgcgc tttgccatcg
     3121 aaagcatgtc aaaagttccc gtattgttat tagcaatcaa agacaacgga atagaaaaag
     3181 tctttcaaac aatcaacacg gaaccaaccg gttttgcctt caattctatc atgaatatgg
     3241 aaatcaatca tcgcaaacat ccaatgaatc cttttgttaa tgcaggagcg attgcaacaa
     3301 cctcgttgat tgccggtaaa gatgcagatg aaaaatttga gcgaatcttg tcatttatga
     3361 aagagatctg tgatgatcct aatatcacat tgaatgaaga aatctatcat tctgaatcaa
     3421 gaacaggtga tatcaatcgc tctcttgctt actatatgaa aggtaatcaa atgatcgaag
     3481 gagacgtacc ggagatactc gatgcttatt tcagacaatg ctccctcaat gtcaccgcta
     3541 caggcatagc aaaattagct gctgtgctgg caaataaagg aattgctcct tggaatggca
     3601 aacaaatcat tactgaagaa agtgctacaa tcgtcaaatc aattatgaca actgctggtt
     3661 tgtatgatga atcaggagag ttttccgtcc atgttggtgt acctgcaaaa agcggtgtag
     3721 gcggcgggtt gatggcagct gtgccaaatc gttacggcat aggtgtcttc agcccggcac
     3781 tagatccatt tggtaacagc gcagctggta tccaattgtt gaaagatgta gtcaaagaac
     3841 tagacgctga catctttgaa taaccattct tgaaagaggc tggggtaaaa gtccaaacgg
     3901 ctcaaagcta cctacaatga ggctgaaatt gtgagcttgt ttgacttttg tcccagtttc
     3961 tttttttatt ggtttttgaa agggcttttc taaataaaaa ggaaacaaaa atacgattta
     4021 cagatatgat taaataaaat cataagaaaa actggaggcg tgtatcagga atgctggaaa
     4081 attacatcga gcgaaatata tttagaaaag tgtatctgtg tgaacaatta tttgaattcc
     4141 aagaaataga catcgaacaa acagctattt ctctaagggt gactacacca acgatccttc
     4201 acgatctaga aagtctagcc gagtgtctag aatattgtat aaaagaacaa gtaagagaga
     4261 agcataagta taaactcgta ttcaaacatg gtattgctct ttctgagctc acacagtttc
     4321 tgtacggtca atcttatttt ttaaagtttt taagttatta ttttaatggt atattcacga
     4381 gtacagaact ggctgatctt gaatttattt ctttgagcaa agtatataca atcaaaaaaa
     4441 tcgtattgga tttttttaaa gaaaatgatt atttgaaaaa taaagaaatc attatcccag
     4501 aatttgactc gcggaacatc ttgctagctt tagtacgtta tgtcaattgg gaaggctatg
     4561 aaaataaaaa tccgtatgta gaagaagctt gtaacgagtt gattgattat gtagagactc
     4621 atttttttaa aagaagatat tcggaagatg aaaagtcctt tatcatacga ggaatagaga
     4681 tcgcaatcgg aagaaaggca tttccccttg gactgaaaga agaagataag aaaagcgcag
     4741 aaacgaagcc gttatttcaa ttagtctgtc aaggattgga agagcaaaaa ggaattctag
     4801 acttacaaga agaagatgtc tattatattt tttctttgtt caacagtaga aattatacca
     4861 atgagaacat ggagctgtta agaaaagatt tcgaagttgt ttataaaaat tttgttctca
     4921 atgacctatc actgaatgaa ttgattttac agattattag tatcgctgaa atcgacgatg
     4981 cagaaaattt tattttcaag caatcctttt tacaatttgt ccgtacgttg tgggcagatg
     5041 gacaagtatt tttaccggaa aggatctatc ttctatcaga aaaagaacaa cttctgtacg
     5101 gaaaaattgt agaaatattg gaagagtgga aaaagaaaaa tcaaatccaa ctaagatgga
     5161 atgaaaattt cattcgaaaa ttcgtcaaaa gtctttcttt gatcaacagt gaacaagcgg
     5221 ggagacagga aatagaaatt tttgttgttt cagaaagttc agtcaaacaa tttttttatc
     5281 gaacccaatt tcatttgtat gcggaagaag gcatcgctga aataaatcct attttatttc
     5341 gttcattaga agaattgcct gatgaatgtc tatgtgcgaa aaaaagagtg gttttgtgcg
     5401 atgctgctag ctatcaaaat ggattagaaa ctgaaaagac aaagatctat ccaatctcat
     5461 taaaaggtgc aaacatccat ttgtatcata ttgctcagga acttcaccta ttaaaaaaac
     5521 atatttcttg atgagacctt aattaggaga ccttctacaa agctttttaa ttgtctgtag
     5581 aaggttttta tttgagtcct gattcgtgcg aagatctact tatataacaa gctttctctg
     5641 aaattttttt aaaatgctat tgacttagag tcaactctaa gtagtagaat agataaagtg
     5701 taacaaatct attttcatta aaagaagata gtacggagga gagaatgtat tatgagtaca
     5761 gtattacatg tattggatgc aatcaatagc cccgcagatg tcaaaaaatt atctgttgag
     5821 gaaatgaaac agttggcaga agagatgcgc caaaaaatct tgcaaaagga cagcgatgta
     5881 ggtgggcacg ttggtccgaa tctaggtgta gtagaattaa cgattgcgtt tcattatgta
     5941 tttgattcac caaaggataa aatcgtttgg gatgtttccc atcagtctta tggtcataag
     6001 cttttaactg gtcgaaaaaa tggttttgaa aaaggacatt accgtgaagt gagcggatat
     6061 tccaaccaac atgaaagtaa acatgactat tttacagtgg ggcacacatc tacatctatc
     6121 agcttagcaa caggtttagc taaagcgaga gatctgaaga aagaatcagg aaatgtggta
     6181 gcatttatcg gtgacggctc cctaagtggt ggcttagctt ttgaaggatt gaacaatggt
     6241 ggtgttttac aatccaatct aatcgtatta gtaaacgata atgagatgtc tattgatgag
     6301 aatcatggca gtttatataa aaacttagct gagcttcgtc aaaccaacgg taaaagcagc
     6361 aataacctat ttaaagcaat gggatttgat tatcgttacg tggataatgg aaacgatctg
     6421 gaaacggtga tccgtttatt tgaagaagta aaagatattg atcatccgat tgtacttcac
     6481 gtccatacgg aaaaaggtcg cggttatcag ccggcaatcg ataataaaat gaaatatcat
     6541 tggcatgtac catttgatct gtccaccggg gaggcaaagc aagtaacttc tggagaaaca
     6601 tttaatatga tcatcatgga ttatttggat aagaaaatgc aaacaagtac agctcctatt
     6661 gccgcagtca atgcagcgat accaggcgcc tttggactga aaacattcca agaaaaacat
     6721 ccagatcatt attttgacgt ggggatcgct gaacagcact ctatcacttt cgcttctgct
     6781 ttggcttctc agggagtacg tccagtcgtc ttccaatcaa gtactttctt gcaacgggca
     6841 tatgatcaat tatcacatga tttagcgatc aacgagaatc cagtgattat tttagtcggt
     6901 ggcggaacga tctcagctag tgaccgaaca caccaaggaa catttgacat tccttactta
     6961 tcaagtatcc cgaacatcaa atatcttgct ccagcaacaa aagaggatat gatctctatg
     7021 ctagattggg cgttcgaaca aacagcgcag cctgtagcaa tccgaattcc tacacatggt
     7081 gtagaacatg gtacagctat cgcaaatgat tatagtattc cagcctatca aacagtaaat
     7141 aatggaggaa aagtagcaat tttagcttta ggcggttttc ttgaattagg aaaagaaaca
     7201 gtaaaagctt ttagaaaaaa aggaatcgaa gcaacgttga tcaatccagg aacgatcaca
     7261 gaaaatgacg tagaaacatt agataaatta aaagaaacac acgaattagt catcacatta
     7321 gaagatggta gtttgtcagg aggatttggc gaaaatatca gtcgttatta tggtgattct
     7381 tcaatgaacg tattaaattt tggtgcagag aaagaattca cggatagcgt tcctgtaggg
     7441 cagctttatg aacgttatca tttaacacca aaacaaatcg ttgaagatag tctgcgtgtc
     7501 ttgaatattc aataattcat tatgctcacc atccagataa ttgtttatac tagtatctgg
     7561 atggtttttt cattataaaa agcaaagggg tggatgttat gtatcagcaa gtagaagaat
     7621 atgtcaaaaa gttccaagaa aaatatccta gttatcaatc agaaaaatta ggtttctttt
     7681 catttgggga tacagaaaaa atggcagacg agttgagcga actagttgta aaaaagataa
     7741 agactggtac atcaagtggt tacgatctgt ataaacaaga gaatgagcct ttaccaaaag
     7801 ttggtcaatt agacgtggtc ctgaatggaa aaaatgaacc agtttgtatc atcaaaaata
     7861 taaatgtgac catcttgcct tttagtgaag tcacgaaaga gcatgcttgg aaagaaggag
     7921 aaggggatcg gacattagct tattggcgga aagtccatgc tgagtttttt aaacatgctt
     7981 ataaagaagc gttgaacata gaatttacag aagagaagct ggtcgtatac gaagaatttg
     8041 aagtaatttt cttttagtaa gaaaggtctg ctgaaagaaa tagctcatta ttgtctcctg
     8101 aatagaaaag ggttacattt ttatatgaag ctgtattttt atataacatt actggaggag
     8161 atattgtgga tacactggaa caaatcaaag gcggagtcgt cgtttcttgt caagctttgg
     8221 aaaacgagcc gctacacagt tcttttatta tggggagaat ggctttggct gcaaaagaag
     8281 gcggagcagt tggtattcga gcaaatacga cagaagatat caatgaaatc aagcgaatcg
     8341 ttgatttacc ggtaataggg attatcaaaa gagattacga tgggtcagaa gtgtttatta
     8401 ctgcgacaga aaaagaagtg gaagaacttt tggagacgca agcagaaatc attgcactgg
     8461 atgcaacttc tcgtaaacgt ccgacagaaa aaacaactgc tgaacttgtc caaatgattc
     8521 acgaacacgg tcggttagct atggcagata tttcgacttt tgaggaagcg cttcaagcgc
     8581 aagaagatgg ttttgatctt ttgtctacga ctttagcagg ctatacagaa tattcacgaa
     8641 agacgcagac acctgatttt gatttattga atcaaatcat agaacaagtc gatatcccag
     8701 taattatgga aggacatact gactctcctg aacaagtaga gaaagcatat gaattaggtg
     8761 ctttttcagt cgtagttgga tctattatca cccgtccgca attgatcact aaaagatatg
     8821 tcgaagccgc aagtaaagca aataactaaa aaacacgcac ggcacagatt cttgacaaaa
     8881 aagaaaaatt gatagatagt ttaaagcaga ttctaagaag atgaaaaaag cattactttt
     8941 cattttgtct tcaagatctg ctttttaacg aattttatag aaagaaggcg aaaccatgtt
     9001 tgaacggagt ccttcagaat tgacagaacg tgaaaattac aagttgctga ttggttcagt
     9061 catccctaga ccagtagcag tagtaacgac taaatcagca aaagatatag tgaatatcgc
     9121 acccttcagt tacttcaata tcgtcagttc gaatcgaccc atcttatcct tggcgatcca
     9181 gagaaaacaa gatgcagtaa aagacacagc aaagaatatt cttgagacaa gagaagctgt
     9241 tatccatatc cttgatgagg acaacgtaga agaagtaaat aaaacagcag ccaatctgcc
     9301 taatgaggaa agcgaactgt acctgactga cttgacttta actgattcag taatcatttc
     9361 agtaccagca ttgacagaag caaaagttcg ttttgaaaca tccttatatg aacatgtcga
     9421 gatacggaat aaaaataaag taacagctga tttatttttg ctagaagtac agaaatatca
     9481 tttaaatgaa acagtctatg atgataagac aggaaggatc gatccaagaa aattgaatgc
     9541 tgtaagccgt ttggcaggaa atgattatgc agcaatagga gaaattttca cgattgcacg
     9601 tcccgaataa aaaggagcac tagttatgtt aaaaataagc tataaaccat cgacagattc
     9661 aaaagaaatg aaaaaagaat atgaaacagt gaatgatttt ctgcaagggc agtacttaga
     9721 agttccgcca ttgcaagacc attttgtcgt tactactgtt actttagatg gtaaggaaat
     9781 agaaatgcca gatcaaacga ttagcggatt gtttaattac tttaacaaat aaatatttat
     9841 tccgaaaagt gaaaaaacat ataaaaaaac acataaaact aaaaaattat caaagaatcc
     9901 tggtgcaact gaaagaattt gcaacgggat tctttcaact tatttgttat ttttttctaa
     9961 aaaatattaa agattgttaa atttcactat ttcatttcaa attccattgc ctaatcatta
    10021 ggaaaagtct atcatgaact cgtaaataga atatagtaat aaccttgtta ggtgaggctc
    10081 ctatacaaac ataggctgtt gcccagaaac gtcgaaagtc gccaatgggt aaaacagaaa
    10141 ctgttggatt aaggcttttt ccaatagcta agaatgtttt ctttacgttg tatagtgcta
    10201 aaactcggac gatgagatga tgctaggtca gaattcttgt tcgaaaaggt ttctgacctt
    10261 ttctttatat ttttgtgact gacttatttg atgagacaaa ctcgctcctc acctgacaag
    10321 tggattgctg tgtgaggagg agatgcggtg aaaaaagatc gattggtgga ggtcgctcgg
    10381 attcacttct ggttgcacaa tacaaccaga aaacacaaca agcaaaactg atctctatca
    10441 tgagagattc atatgtcgat attccaggtt acggaatgaa taagataaat gcagcttact
    10501 cttatggggg agttgattta ttaaatcaaa cattaaagga aaatttcaaa tttgaagctc
    10561 cctattatgc aagtatcaca tttcaagatt ttatcgattg cgttaatgaa ctgtttcctg
    10621 gtggagtcaa gatcgatgca gaaaaatctc tagatttaga tggcgtatat ataaacaaag
    10681 gagagcaagt gatggatggt aatactttac tccagtatgc tcgatttcgg gaagacaaag
    10741 aaggagactt cgggaggatc agaaggcagc agcaagtgat caaagctatc tctcagcagc
    10801 tgaaagacgt aacttcaata ttcaagctac ccaaagcggt tgggaaatta cttggtagta
    10861 tccaaacgaa ccttccagaa agtgtgcttc tggattgtgg tatggatttc ctcaaagaca
    10921 tatgttagaa taaacaggta taaatagtga aataaaggaa tgacaagcaa gagaaggagg
    10981 aaaaaatgaa aagaagtgac aagcacggca aaaatcgaac aggcgcttat attgccggcg
    11041 cagtgatctt aatagcagct gcgggtggcg gttattttta ctaccagcac tatcaagaaa
    11101 cccaagcagt agaagctgga gaaaagacag ttgagcaatt tgtccaagct ttgaacaagg
    11161 gagattataa caaagctgca gaaatgacat caaaaaaggc ggcaaataaa agtgcattat
    11221 ctgaaaaaga gatcttagat aaataccaga atatatacgg tgctgctgat gtcaaaggac
    11281 ttcagatatc gaatctaaaa gtagataaaa aagacgattc tacttacagt ttttcatata
    11341 aagcaaagat gaatacctca ttaggtgaat tgaaagatct ttcttataaa ggaacattag
    11401 acagaaatga tggtcaaacc acgatcaact ggcaacccaa cttggttttt ccagaaatgg
    11461 aaggaaatga caaagtaagc ctgaccacac aagaagcagc aagagggaat attatagatc
    11521 gaaatggtga accattagca acaacaggca aactaaaaca attaggagtc gttccaagta
    11581 aacttggaga tggaggcgaa aaaacagcca atatcaaagc gattgcttcc tccttcgact
    11641 taacagaaga tgctatcaat caggcgattt cacaaagctg ggtacaaccc gattactttg
    11701 tcccattgaa aatcattgat ggagcaacgc cagaacttcc agctggagct accattcaag
    11761 aagtagacgg cagatattat cctttgggtg aagcagctgc tcagctgatt ggttacgtgg
    11821 gagatatcac agcagaagat attgataaga atccagaatt aagcagtaat ggaaaaatcg
    11881 gtcgctcagg tttggagatg gcttttgata aggatcttcg tgggacaaca ggtgggaaat
    11941 taagcattac agatgcagac ggtgtagaga aaaaagttct gatcgaacat gaagttcaaa
    12001 atggcaaaga tatcaaattg acaatcgatg cgaaggcaca aaaaatagct tttgacagtc
    12061 taggaggaaa agctgcatca actgttgcga caacgccaaa aaccggtgat cttcttgcgc
    12121 ttgctagctc tccaagctat gatccaaaca aaatgacaaa cgggatctca caagaagatt
    12181 acaaagctta tgaagaaaat cctgaacatc cattcatcag ccgatttgcg acaggttatg
    12241 ctcctggatc tacgtttaaa atgattacag cagcaatcgg tctcgacaac ggcactatcg
    12301 atccaaatga agtgttgacg atcaacgggc ttaaatggca aaaagacagt tcttggggat
    12361 cttttaaaac aacgcgtgtc agtagtgatg tatcacaagt agacttaaaa actgctttga
    12421 tctattccga taatatatat gcggcacaag aaacgttgaa aatgggtgag aaaaaatttc
    12481 gtacaggctt agataaattc atttttggtg aagaccttga tttgccaatt agtatgaatc
    12541 cagcacaaat ttctaatgaa gatagcttta actcagatat cttgctagct gatactggat
    12601 atggacaggg cgaacttcta attactccta tccagcaagc agcaatgtat tctgttttta
    12661 ccaacaatgg cacacttgtc tatcctaaat tgattgcaga taaagagaca aaagataaga
    12721 agaatgtcat cagcgaaaca gcagtacaaa cgatcgtgcc agatctgaga gaagttgtgc
    12781 aagatgtaaa tggtacagca cattctcttt ctgctttagg gattccgttg gcagcgaaaa
    12841 ctggtacagc ggaaatcaaa gaaaaacagg atgtaaaggg gcaagagaac agtttcttgt
    12901 ttgctttcaa tcttgataac caaggttata tgatggtcag catgttggaa aataaagaag
    12961 atgatgattc agcaaccaaa cgagcatcgg aactattgca atacctcaac caaaattatc
    13021 aataaaaaat tgctttttaa gtacagtaaa aggactgtgg cgttatcgtc acagtccttt
    13081 tttcaaataa acggttttca cgatgtctct ttcacaactt ctttttgatt tactcacact
    13141 tcataaactt tttttgtaaa atatttgaga acaagtggag aaataagtgt acttaataaa
    13201 atcacgataa ctaaaggtga atagtactgt ggttcgatca attgactttg ctgtccaatt
    13261 tgtaaaatga tcaaagccat ttctccacgc gaaatcatac cagcaccgac catcaatgaa
    13321 ctatgttgag aaaaaccggc aatctgagca ccaaaataac ctccaagcaa tttggtcaaa
    13381 atcgctacaa gcgtcaatat agaaataaag agtagttgtt cactaaagtc agaaaaatca
    13441 acttctaatc ctacactgac aaaaaatact ggtataaata ctgcatatcc taaagcctct
    13501 acattgtggt atacttcttc tttaaccttt gtctgactga cagctatccc agcaaaaaaa
    13561 gcaccaatga cagagctaag cccaacaaga tccgctacgt aagacattcc taaacaaata
    13621 actagcgata cgatgataat agaagaattt gcgtacactt tttctgcaaa tgaaagtagg
    13681 aaaggcgcaa tccatttgac taataagaaa ataaaaacaa aataaattag ctgttcaatc
    13741 aaaacaagcg gtaaagaagc attgctagaa gcttcccccg ttagaaaaga taggctgaaa
    13801 cttagaacta aaacgactaa aatgtcatct accacagaag cacccaaaat tgttgatcct
    13861 tcttttgtat tcactacgtt tagttctttt aatacttcta ctgaaatact gacagaagtt
    13921 gccgcaagaa taatcccaaa gaaaatcgct tcatttcccg aaacttggaa tgctttgcct
    13981 gtcaaccagc caaaaacaac cgggaagatg atcccaagca aagcaacgaa cataccaggt
    14041 cggaaatatt ttttcaaaag agataaatca ctttccagac cagctaaaaa cattaataag
    14101 atcacaccaa tctccgaaaa gtcatggact agaatatcag gtttgaccca attcaatcca
    14161 gcttggccta gcaaaacacc aacaagtaat tgtccaatca ctgctgggat acctaatctc
    14221 cgcgagatat gtgagccaat cgttgttgaa atcaatatca ggcataaaat accaataaat
    14281 tccattctca tcattccttt gtataaaata tagcccgaac ttagcataac atacattatt
    14341 gaaaaaatag gaaaatatac tcacaaaaaa accaacaatc caaaagattt ttttggattg
    14401 ttggttttta tacggtctat tttaataact aaaaatgatt aacgtatagt catttttctt
    14461 attctgatga aattgataat ctcctggatg cccatgaaaa tcaaaataaa gccgaatatt
    14521 tggaatagaa gcatcaaact tttgaatgga ttgaacaata agacgaatcc gccaatcaac
    14581 aacagaatac tactataaac actccaactt ttagcttttg tattcatgct gaagaacaac
    14641 tggaacaagc tgttgatgat aacagctaat ccaagaagaa tcggcaggat agaaacgatt
    14701 gcaggtgcaa aagcatagac gatcagagct aagatcagga aaaaaagacc actgaacaga
    14761 ccgatactcc aagaacctgt tttcctttta aaacggtaag cctcaaaaag attcaacaac
    14821 ccaagcaaaa caagataggc ggtgatcaga taaccgataa aatgaaaaac agcagaaggg
    14881 tttagcacaa tcgcaatccc agaaataata taaacagcag ctcttaataa ggcgtatttt
    14941 tgaatatttt gtttaatcgt ctcaaacata taagaaaacc tcctttgact tattttaacg
    15001 tgaactagtc attttaccaa attaaatgtt acaaaatgag cgaaggagaa gaatatctct
    15061 ggaaaaatca acttctgagg atcttttcca tttttttccc ttttgctagc tcatcgatca
    15121 ttttatctaa ataacgaatt tctttcatca agttatcctc gatgtcttct actcgtacac
    15181 cacaaacaac acctgtaatc aaggaacgtg aaggattggg gacaggggct tccgcaaaaa
    15241 attgttcgaa agtcacttgt ttttgtagtt gatattctag ttctgtttgg gaataacccg
    15301 tcagccaaca aatgatttca tctacttcct cttttatacg atcttttctc tccgcttttg
    15361 ctacatacat aggataaacg cttgcaaaac ttgtcgtaaa aatacgattt tgcgccataa
    15421 tcagtcacct ttcttttttt caaattattt actacttata gtatataaga aaaagagaag
    15481 aaaaagcgat tattcaaaaa aacaaaaagg attgtgacaa aattcttgtc accatccttt
    15541 tttccgctct ctgacttttt acaatcttca tagctcaagt gcgcatcaga taatcgaatg
    15601 ctcctaaagc tgcagtagct cctgaaccca ttgaaacaat gatttgtttg tatggactat
    15661 ctgtacaatc accagctgcg aaaacgccgg ggatattcgt tgcgccattt ttctcggtca
    15721 ctatttcatt tcttggattc atttcgatcg ttctatctag ccaatccgta ttagggagta
    15781 agccgactag aatgaatact cctgaaacag ctagtgtatg catctccatc gtacggcgat
    15841 cgatatagga gagagattct acctgattcg taccgctgat ttctttcgtc gctgcatgtt
    15901 tgataacagt gacattcgca agagagacta gtttatcctg taagatttga tctgctttta
    15961 attctggtaa gaattctaga acatatacat gttttgctaa cccagccaaa tcgattgctg
    16021 cttcaacacc agaattacca ccaccgataa cagcaatctc ttttccttta aataaagggc
    16081 cgtcacagtg tgggcaataa gcaattcctt tatttttaaa ctctttttcg cctggtacat
    16141 tgatagaacg ccagtgcgca cctgtcgcga tgatggcggt ttttgcttta agtgatatac
    16201 cgttagctaa gccaatatta ataaaatgtt gctctttatt taaggaaacg gcctgttgat
    16261 tcatcataat gtcgataggg taactacgca catgactttc tacttgctgc atcaattttg
    16321 gaccttctgt gtaagaggta ccaatcatat tttcaatccc tagtgtttca acgacttgtc
    16381 cgccgaaaga atcagcgaca agtcctgtac ggattccttt acgtgctgca tagatagctg
    16441 cactactagc tgctggtcca ccgccaatca ctaatacatc ataaggttct ttttcaaaca
    16501 actcttcttc aatcaaaggg ccagtgattt tttcaacaat ttgttcgatc gtcatgcgtc
    16561 cgctactaaa ttcttctcca tttaaaaaca cagttggcac tgccatgatt tttttatcat
    16621 cgatttcttt ttggaacatc ccaccttcaa tcatcgtatg agtaatattt ggatttaata
    16681 cagctaatat attcaatgcc tgtacaacgt cggggcaatt atgacaagtc aagctaacat
    16741 aactgtcaaa atgcaatgtt tgtgttattt ttttgatttt aagactcaaa gattcactga
    16801 ttttcggtgc ccgtccacta atttgtaaca tagccagtaa aaaagaggaa aattcatgac
    16861 cgagggggat accagcaaaa acgattccac tttctttgcc aactggattg atagaaaaac
    16921 taggtgtgcg catcgaatct cttttttccc aagagatttt aggagacatt tctgctattt
    16981 ccttcgtaaa ttcagctagt ttttgactat tttcactatt atccaaacta acagaaaaaa
    17041 caataggaga ttcaatcagt gctaaatatt gttcgagatt ttgtttagtt tctttgtcta
    17101 acatatccac cacctaaatc tttccaacaa gatcaaaact tggtttcaat gtttcgccac
    17161 tttctttcca ttttgccgga caaacttcac ctgggtgggt acgtacatat tgagcagcgc
    17221 gaatcttatc aatcagtgca ctagcgtcac gaccgattcc atctgcattg atttccatag
    17281 cttgaacgat tccgtctgga tcaataataa atgttcctcg ctgagctaat ccttgctctt
    17341 catccaaaac atcaaaaata cgagaaatct gatgagacgg atcaccgatc atcacgtatt
    17401 cgattttacc gatagcatct gatgtatcat gccatgcttt gtgagtaaag tgggtatcgg
    17461 tagagcaaga aaagacttca acacctaaag atttcaatgt actgtattgt tcttgtaagt
    17521 cttctaattc agtaggacaa acaaaagtaa aatcagctgg atagaagcaa agaatactcc
    17581 agttccccat aagatctttt tcactgactt ctaaaaattc tccttgatga taagcatgtg
    17641 cattaaattc ttcgattttt ttcccaatca atgacatagt aaaacctcca ataataatta
    17701 tttatttata attattgtaa tttagttgga gttgcaaagt caatggatat tttgttattt
    17761 aataaagatt actcaaaaac gaaggttaat tataaaaata gaagaggtcg caagaaaatg
    17821 ctactgctgg aaatactcgg gataacgttg agtaacagtt tcctgttctt tcagcatcat
    17881 gtgtaagtgc aatcccgttt gaaatcgagc ttcttctggt tttttatcaa ctaaagcctt
    17941 gagtaaatca taatgttgtt ccatgatcgt ttcccatgga agcccctgaa tggtcactcg
    18001 taaccagcgg aaacgttcta aatgggtatt atggtcatct aaccagttcc aaacttcttt
    18061 tcttcctgca atttcgaaac aagcttcatg aaataaatta tctgcacgga aaaaagcgcg
    18121 ttcgtcattt attttgactg ctttttcttg ttcttctaaa atcgtttcta gcactattcg
    18181 tgtttgtgtg tccaatttgg aacaacattc aatcatgatt tgctcttcca actgttctcg
    18241 gacaaagcga gcattttctg cagaaacaag attaattttg gaaacatacg taccactttg
    18301 aggtatcgta tacacgagtt cttgctgacg tagttgaatc aatgcttcgc gaatgggagt
    18361 ccggccgata gcaagatgtt cctccagtgc cttttcagat attttccttc caggttctaa
    18421 gtcagcatag ataatttgtt tacgaatcgc ttgataagct tgtgattgca agctgctttt
    18481 catctttatt cctccaaaat catttctatg aacgtagtat aacagaagag gtctgaaaaa
    18541 caatctggta tgtcacatta actagtaaat ttgtataaat agtaaaattt tggttgacat
    18601 agcgattttt attgcataaa attcacttga atacaaaaac taatatgtta gtttaggagg
    18661 ggaaaaatga ttgatttaaa caactggagg aactttatcc ctgaattatc tcaaaaagaa
    18721 gtagaaatac ctcattttga ccgtgcatca ttaagagaaa atggacaaaa aaatcctgtc
    18781 tggatccatt tcggtggcgg aaatctttat cgaggatttc atgcagaaat cgctcaacgt
    18841 ttagcggatc taggcgaatt gtccactggt gtcattgttt gtgaaacatt cgatgaagaa
    18901 ataattgaaa gggcttacca agattttgat gatttgattc ttgaagtggt catgccaagc
    18961 agtggagagt tcgtcaaacg tatattagct gcgacagctg acagtctgta ttgtcatcca
    19021 gattgttctg aacactttga taagatgatt cgacactttg aaaatcctag tttacaatta
    19081 gttacttttg ctattactga aaaaggttat cgattgaaag atacaaataa taaatatata
    19141 ggaccgattg cagtcgatat aaaaaatggt ccaaagaagc catcacatac aatgagtatt
    19201 cttgctgtgc tactttataa acgtttttta tctggagctt ggccgattgc acttgtctct
    19261 acagataatt tttcacaaaa cggtagtcaa ttacgtactt ctttgctaac gatcgcaaaa
    19321 gaatggcaaa aggaaggtta tgtacaaaat acatttatcc aatacttatc gaatgaagaa
    19381 attgttgcgt ttccatggac gatgattgat cgtatcaccc cgaatccttc cttgtcagtt
    19441 agcaaaatgt tggcaaaaga gggcttttca aatatgtcga ttttccaaac cgcaaaagga
    19501 aataatattg ctccatttgt caatacagag gacatccatt atttaatcat ggaagatcat
    19561 tttcccaatg gacgtccgaa attcgaaaaa gctggggtat tcttgactga tcgtgcaacg
    19621 gtagataaag cagatacgat gaaggtgacg acttgcctta atcctttgca tacgtcttta
    19681 gctattatgg gttgtttact ggggtatagg agcatagctg aggagatgaa agatccagat
    19741 ttatcgctgc tagtttctga aatcgggtac aatgaaggcc tcccagttgt ggagtctcca
    19801 ggtatcattg atccaaagat ctttctggaa gaagtaatta ctgtgcgctt ttctaatcca
    19861 aatattccta atatgcctca gcgaatcgcc acggatacct ctcaaaaggt ggcgattcgt
    19921 tatggtgaga cgattaaaaa atatatggaa atagaagtta aagacccgca gacacttgta
    19981 ttcataccat tgactctcgc tgcttggctt cgatacctta ttggcattga tgatcaagga
    20041 aaaccattca tcccgagtcc agacccacta ttgacagacc ttcagcataa gctttccgat
    20101 attcaactgg gtacgcaaga tacatcacat attcatgaga aaatggctcc tattttgtca
    20161 aataaagaaa tttttggtgt agatctttac caagcagagc ttggcagaaa aatagaagaa
    20221 atgttcatca agatggtagc aggaccaaat gccgtacgaa aaacattaca ggattactta
    20281 caagaacatg gaggaaattt atcatggaaa tgacttttag atggtacgga tcacaggaag
    20341 aaaagattac cttagatgaa atcagacaga ttcctgcggt caaagggatt gtaggcacat
    20401 tgatggatat accagtagga gaggtatggc ctagggagag gatcaaagaa ctaaaagaag
    20461 aaattgagtc tcgtgggtta acactaaaag taattgaaag cgtcaatgtg catgatgcca
    20521 ttaaaattgg attaccttcc cgtgagcagt atatcgaaaa ctataagcag acaatccgaa
    20581 acttggcaga atatggaata gaagtcatct gctataattt tatgcctgta tttgactggg
    20641 tgaaatctga ccttgactac cgcttagaag acggctcatc cacgttagct tttatatctg
    20701 atgatattcc agatgatcca agagaaatcg tcgcgcgcat cgaacattcc agcaatgaat
    20761 ttggattgcc gggatgggaa cctgagcgct tagctcctat caaatccttg cttgaagcct
    20821 atgcatcagt agacgaagaa aaacttcgag aaaattttgc ctattttctc caatcaatca
    20881 tcccaacatg tgaagaggta ggggtcaaaa tggctgtaca tccagatgat ccgccatact
    20941 cgatttttgg attagctcga atcgtaaaga atcgagaaga tttagattgg ttgtgtcgca
    21001 tagtagacag cccttataac ggaatcactc tttgtacagg gagtattgca gaagatccag
    21061 aaaatgatgt atatgggatt ttagcagaat tttgccaaag agatcgaatc ccatttgcac
    21121 atgttcgaaa tattaaattt ttagctaatc gagatttcta tgaggcacca cataaatccg
    21181 aatacggttc attagacatg taccgcatac taaaaacaat gtatgacaac ggatttaacg
    21241 gatacatccg tcctgatcat ggaagaatga tctggaatga gacaggaaga ccaggatacg
    21301 gcttatatga tcgagcatta ggtgctgttt atctaaatgg attatgggaa gcaatcgaaa
    21361 aaggaaacaa aagtaaagat aattagtgaa aaaaactgta attttattat gtaaataaga
    21421 aggatgttca aattcttgat acataaatcc ggaggttgtt aataagcatt cggatttatg
    21481 tatcttattt ggtctaggaa gtaacttgat tttctttttt cgattgatcc atgagtgctt
    21541 cttcttttaa ttcaaagata tattctgaga tcctcataat cacttttttc cagtcttctg
    21601 gtgtgatgat tccttttaaa atatagattt ttttgatatg agaaggcaat tgtttcaaag
    21661 ggcagtcgct gataacgatg ttgcagttct ccataaaatc attttgtatt ttgatcgatt
    21721 catttgaaaa aatttgttct atcttaacga tcagatcttt actgatataa aaatcacgcg
    21781 tatagttcac accaatcaca attcgcggct gttttttgta gcgatcatac aaatgtacta
    21841 ggtcttcaac catccattgc acattttcct tttctaaaat aactggtaac aagtgttgat
    21901 tttgttctct gaaatccata taaaattgtt caatggcggt ttggacttct ttcaatacgc
    21961 tatgttcttg ataaagcatg aaagtaggaa ggggcgtatt tggttgattg aagttaagaa
    22021 atccattata aagtttattt cgtgcgaatg ccaatgagta aagaatcttt tctttttcag
    22081 gtataaacaa atcaaataca gtacaaaggg actccaccat ttctcttgaa taaagaacat
    22141 gaggaacgtt tgcttttttt aaaagtttgt attgctccaa gcttgtttct tccgattcga
    22201 ttttcgtgat agaaagtcga gctaaaatat tcactaagat gcgttgttcg cgacaaattg
    22261 gcagattagg tcgcaaaata tctacagaga gatcaataaa aaagtcaata tattcatact
    22321 ctgtgaattc tttttgtacg tctttcacag aagttgcagg aaaattctct ttacatattt
    22381 ttaatagaaa atataattta tctatcatac cgctatttag tcggtcaagt acttcttttt
    22441 taaaaaaagt attcatcata aaagccacta cttgcggatc ttcatcataa aattctgtag
    22501 cagtacttga aaagatgttc caataaatat ctaatgctgc atattgaatt tgtacctctt
    22561 ttccagaaaa agactttctg ccattattag aaaaggagat agacaaaccg tactatgcta
    22621 aaaatgcatt aatctttttc attttcgtat acaaataaga ggcactatag tttgtttgct
    22681 gactgaaact gataatcgaa tttgtctgac agctgaacaa agaagtcaag atctgataac
    22741 tgactgattc gttgcagtat cttccaaata gacgataata gcagtaagcc ggatcaattc
    22801 cttcaaaatt caactttaaa aaaggttttt gcttatcaat atgcagttct gttagattga
    22861 gacgctcaaa gtcttgttct aattctttga tgtatagtaa aaaatttgat tcagacatat
    22921 ggaaatgatc tgctaaatat tgtgtagtaa taaagaaacg atgttcagat aggaaaagaa
    22981 aaatatctaa ttttgtattg ctttttttct tcaagataaa cgttttaatc gcttcttgag
    23041 tttgcatgaa agaggctcct ttttacttaa attgttaaaa cttgaaaata ttactgtttt
    23101 tttcataaat ttagacaaaa aaacagtaaa catataggta gaatctctac tatcttattt
    23161 cttgcataca aaaaaatcaa ggagtgagag agttatgaga tattcaacca tcttaatatc
    23221 aacatttgca gccttaagct tatcgcttac tataaatcag acagtccaca cagaggagac
    23281 agaaacagcc aaatggacag aaaccaagtg ggccaacgtc aatcgaatta cctttcagga
    23341 tgaattttca tccttcaatt caggagtcgc aaaattaaga aaaggacaaa cagcgaccat
    23401 caaagcaaaa attgactatt cgggagactt agcacctata ctacaatcag ctgttacatt
    23461 tgagaatgtt gatcccactt tgtcgattgg ctatgacgag aacactttga ctatccaaca
    23521 caagcaagcg atatttacct tcaccgttac attaaatcag gacttgttaa aaccagcgct
    23581 ttttactgtc aaggtatcag atggactacc gaacgatcat catttaacgc cgtatagtca
    23641 gcggcagaca attgagcaag attcagccat cgactcagga ggagatcttg ttgaagaacc
    23701 aacagataaa ccagaaaatg aaaataagcc agaagtaccg cctacagaaa atccagacgg
    23761 agaacagaaa ccggaaatag aacctggaga agaacccgat acagaaactc aaccagagcc
    23821 agacaataaa tcaaaacctg aaataacccc tggagaaaaa ccagatgtaa ctccagaacc
    23881 agatacggat tctggaaacc aaacagtacc ggaaacaaat ccagacacag ataatgaaac
    23941 tgaaaatcca gaaaaaccag aggtagatcc agaagaaaaa ccagatgtga ctccagaacc
    24001 agatacggac gcaagagacc aaggaatacc agaaaaaata aacaaaaaaa caattcaaga
    24061 ggatggaaaa aaagagagta aaaaatcaaa tctagcaatt ctaaaaataa atgaagagca
    24121 gctgaataaa aaatcacgaa tatttgatag cgcccaatca gctgaaacct taaaaagtag
    24181 caaggataca acctttgctg tcctgaaacg aaaaataaac agctaccaaa atccggagag
    24241 tcacaaaaca aagttatact gtggagcgga atcattctat taagtatagc aacaatgctt
    24301 tctgcaaaaa gatttaaaca aaacagatct ctctaacatt tgaataatgt ctgtgaacgc
    24361 atttttcaaa taaaaagaaa tatccagaaa atgaccccaa aataccagat tgaaatctga
    24421 tatttggggt cgttcatact ttatctttct tcatatttat tttaagggat atctgtttct
    24481 tttggaagat ggttcgggat ctttttcact ttcaatacat ctaaaaagaa ggtaaagatc
    24541 ggaatgccga caatcaagcc ccaaacgcca aagaaccgtt cgctaacaag taagaccaca
    24601 aatgtataaa aaatcggcag ttctgttttg ctggacatca acttcggatt cagcacataa
    24661 gactcaatta gatggacaat caaaatcgta atcaggatat aaacaacatc tcggatgcct
    24721 ccttgggaat aagctatgaa agatagagga atacaagaaa tgataacacc tgctactggg
    24781 atcagactta aaatgaaaat catgatcgct aaactcagca gctgatgaaa gccaaataca
    24841 gccaaggcga ttgtagtgag caacgtattt aaaagagcga caacaaattg agcttctaat
    24901 accaaaccaa atgtattgac aaacttatcg gcaaaatagt aaatatcttg aaagaaccaa
    24961 gaaaattcac tttttaaaaa caatcttgag aatacaattg tttttttctt ttcaatcata
    25021 aaaaagaaac ttaagatgaa tgacatagca aacgccactg ccatttttcc tatatcctgt
    25081 aaatagccaa gcgcaatcga agcgccattt tgaagctgtt caaaaaaatc gtttcgctca
    25141 atataagtat ggatatattg taaaacagga tctgcatctt ttggctgatg ctcgtaaaaa
    25201 tcaataacgg actggatcat atcaaatgtt tgatgaataa gcatcgggat atactttgtg
    25261 aatgccagat agataaggta aacgatcagt gtataaatca tgatagctaa aacacctgta
    25321 gggattcgga caaatcgctg aatgatttga acaagatgaa tcatcaaata agtaaagata
    25381 aatgtgagca aaatggttgt aatcatactt ctaactaaga aaagaacaaa aacgacaaat
    25441 aataaaacag tgagacggcg tattttttca ttcgccaaaa aacgctgata aacggtcaaa
    25501 tcgacaacct ccaaataaca aaaaataaac acgttattca ttatatgatg gaatatgaaa
    25561 ataagaaaga gaaagagcaa aaagaaaaac cgctaaatga cgttatctta tgagttggtt
    25621 ttcctcataa aatcgttcat ctagcagttt cagccctttt tctggtcttg atgaaaatta
    25681 tctagcattt ttcgggtcag atgttgcagt gtatccaatt cttcctgttc aagtggaaga
    25741 taagacttga tcttttgtgg aataccttct gcttgttttt gcaacgcagc accttgagga
    25801 gtcagcgtaa catttacgat tctttcatca cccgtttcac gctgacgatt aatataattt
    25861 ttttctgcta acttttttag taagggagtc agcgtaccag aatccagaca caaatcattt
    25921 cctagctctt tcactgaagt ctttttcttt tcccataaaa ctaacatcac aagatactgg
    25981 gtataggtaa gattcagcgg cttgaggagg gaagtgtagt gtctaatgat ttccttagag
    26041 gcggcatata aaggaaaaca tacttgatta actaaatata gatcttccat ttttccacct
    26101 ccgctgtttt actatagtgt atcatagcgg atttacacaa aaaagtctat tcactaactg
    26161 ctttcagagt aacttctata tttccagctg tagcttttga atacggacac actttatgcg
    26221 ctgcctctaa taattcttgc gtttcttcca gagaaagacc ttcgatagat ccttcaatct
    26281 ctacacctaa gacaaagctc gtttcacttt ctgaataaag ggaaacttgt gcactgacag
    26341 tagaagcccc agagatattt ttttgctgtt tgatcaattc taatgcactg ttaaaacatg
    26401 cactgaaacc agctgcaaac aattgttcag ggttggttgc attttcgacg cgtttaccag
    26461 gagctgcaat attcaattcg aatgtacgat cgggattatg aacttctcca gagcgtccgc
    26521 ctgtgttgat cattttcgta gaatacattt ttttcataaa attaccacct ttaattatat
    26581 tttatacaat tgaattttac acaatataat tgtggaaagc aaagaaatgg attggcataa
    26641 gataaataag agaaagcaca tgaaacagac gcgctttctc gaatagcaaa catatacaaa
    26701 aaaatatcag agacaaaaac taatctcatc ataaacatta catatatttc tcaaaaagtt
    26761 gttttctttc ttcaaaactt acttcacgat ggacctgatg aagcatccat aaataaccaa
    26821 aaggatctct gaacattgag ttagtcacac caaagtccat catttctgtc atttcttgaa
    26881 tctctacaca gccatttcgt aatgctttat tatgtgtgtc ctcgatatca gagacgatga
    26941 tattgaacca gatcgtattt ggatgttcag cagtaggagc agtcaaacca agagacgtgt
    27001 tttcatccag catatggaat ctcgtgccat acaaggtaaa aatgacttca ttattgcctc
    27061 tagggtaatc tgttgcctca atcagttgta tttcatcaaa gattgtttga tacagttcta
    27121 gtgctttcaa actatcattt aatacaaaat ctatttccgt tcctttatgc ataaagatct
    27181 cctctccttt tcagtcgaac agttactaga ttcttttatt ataccagtta aagaaaacgc
    27241 attcaaaaaa atagcggatt tacttaccaa ctcattcgat tggatgataa gtaaatccgc
    27301 gtcccattac ttctgctacg ctgctgacaa tgacaaaggc acttggatcg atttcttcta
    27361 tcaatggttt cattcgggaa aattcatgat cagtcacaac gatcaataaa acctcttttg
    27421 actttcgatt atacccgcct tttgcctcta gcagggtaag gccacgtcca atctcagcac
    27481 ttaagcgaag gcggatgtct tccaaataat gttcactcat gaccataaca gatttctttc
    27541 gattgatacc tgtttcgata taagtcatga caattgaagt gattgcaata gataaaatag
    27601 cataaagaaa ctcttctatt ccaaaaacaa acagattaag agaaacaacg aatgcgtctg
    27661 taaaaaataa gccaatcgaa gtattcaagt ggaaatattt tttaaaaatg aggggaggga
    27721 tcgtcgtacc gccgctagaa gagttgtttt tatataaaat cgcaactcct aaagcaaaaa
    27781 tcgcacttcc aaaaatgaca gacaacaacc gatctgaagt aatcattgtt tctggaacta
    27841 ttgcaatgat taaaggaaaa aacaagcttc cgaaaagtac tttaaaaaag ggttttttac
    27901 ctaaaaatag aagagctagt accaacataa cgatattcaa cactaaaata actatagctc
    27961 ggtcaaatcc taaaacagct tccagtaata ttcctagacc gctgacacct ccagcagcaa
    28021 tatgatgagg ccctaaaaac atattgatag cgactcctaa tatgaagagg gcaaaaataa
    28081 tcttaatgta tgtccatata aattgtctcg tttctttcat ttatccactt ccttaattca
    28141 ttaataatac tcttatctta ttataactta cttttcacct taagttaact ttaattaata
    28201 aaaaaattag gctagtctaa attattatgt ttcattcttc tagaatatac ttctacgtta
    28261 aaaactgtta aaaaactttt actttagaaa attggagaaa ttgatcgctt aagataaatt
    28321 caggacacta aatagtacag gaagtagatt aaaactttta aaagttttaa gtctatctat
    28381 ttggcaagtt taaaaaataa aaattcggta tcaaaaaact gttaccgata acatttttga
    28441 aagcgttgtc ttttgtttct ctatcactta tagtgatgac gtcatattaa aacacaagga
    28501 ggaaatcaat ggatacagct gcaatttatc atcgaccgga aagtgaatat gcatttctat
    28561 atacgaatac aagatttcgt attcggcttc gtactaggaa aaatgatatc aaaaaagtat
    28621 atgtgctttg tggagatcca tataccataa caactgaaaa atggtatcaa aaacaacgac
    28681 caatgaaaaa atgtctaagt accaatgttc atgattattg ggaaattgaa gtgacgagcg
    28741 aaacaaggcg cttacagtat gcgttccatg ttgttgggga agataacacg gactgttttt
    28801 acggtgatca aggaattttt ccttatcgag aaaatgtgat tactgaacca aatttttatt
    28861 ttcgaatacc ttattttcat caaatcgatc gattcactat tccagaatgg gtaaaagaaa
    28921 cagtatggta tcaaattttt cccgaaagat ttgcaaacgg ggacaaagca aatgaccccg
    28981 aacacacttt accttgggga agtaaagatc cagaccgaga agacttcttc gggggagatt
    29041 tgcaaggtgt gagggatcat ttggattact tgacggacct tggcgtcaat ggcatctatt
    29101 tttgtcccat atttaaagcc acatccaatc ataaatacga tacgattgat tattatgagg
    29161 tggatcccgc ttttggcgat aaaaaacttt taaaaaactt gattgatgaa gcacataaaa
    29221 gagggatacg tatcatgttc gatgctgtat tcaatcatat gggtgtccat tcaccacagt
    29281 ggcaagacgt tctcaaaaac ggagaaaaat cgatctataa agactggttc cacatccgtt
    29341 ttttcccagt agattcttat cagatgactg acttaccaga aactgctgaa aatatcccat
    29401 atgatacttt tgcttttact ccattcatgc cgaaactaaa tacagcaaat ccagaagtgc
    29461 aaaaatattt gctggatatc gcaacttatt ggataaaaga attcgatatt gatggatggc
    29521 gtcttgatgt tgctaatgaa gtcgatcatc atttttggaa aaaattcaga gaagcagtta
    29581 cagaaatcaa accagacatc tatatccttg gagagatttg gcattcctct caagcttggc
    29641 tgcaagggga tgaattccat gcagtgatga attatgcatt cacagattcg attaaagatt
    29701 attttgcaaa aaagaaaatc acagcttcac aaatggtcag tggaatgaat catcagcaaa
    29761 tgctttacag agatcaggtg aacgaaggaa cattcaactt attagattct catgatactg
    29821 ccaggatctt gaccctttgc caaggtaaca aagaattgat gaagtctgtg atggccttta
    29881 tgtttcttca aaaagggtct ccttgtatct attacgggac agaaataggg atgactggtg
    29941 aagatgaccc ggattgccgc aaatgtatga tatgggagaa ggaggaacaa gatctagagc
    30001 tattcggttt tataaaagaa cttgtttctc taaggaaaca gttgtctaag attatttctg
    30061 aaggttctac tcagtggcta atagtatatg atcgtgaaga taagctctat ttcacacggg
    30121 aactagaggg acagatcatc tatgtttact ttaatcagtc aaaagaacct tgggtagtag
    30181 aacaggaaaa tgaggtgatt cttagtcaaa attgtcagtt gttagaagac gggaaagcag
    30241 agatacgaca atacggattt ttgatcatgt gttgaaaact gttaccggta acaacaagaa
    30301 agcgtttgca aatgaagatt tgtgcccatt ataataaggg tgtaacagca cgttgaatac
    30361 acaggaggaa atgaagatga aaaaaagaag tgtgaaacag ttgggtttag ggttaatctc
    30421 tttaggaatc ttaggcggat tagcggcttg tggcaatggt aactcttcag cagaagggga
    30481 agccgatgaa ggaaaaacgt taacagtttc cgttgattct ggatataaag attatataaa
    30541 cgaaataaaa aacactttcg aaaaagaaaa cgatgtgaaa atcaagctag tagaaaagga
    30601 catgttcgat cagttggaat cattagcgtt agatgggcct gctggaaagg gacctgacgt
    30661 tatgatggca gcatatgacc ggatcggtgc gttaggacag caaggacatc tggcggaagt
    30721 aaaactggga aatgaagcag cttacgatga aacagacaaa gcccaagtga cttatgatgg
    30781 aaaaatctac ggagaacctg cagtaatcga gacccttgtt ttgtactata acaaagattt
    30841 agtcgataca gcaccatcaa cgtttaaaga tttggagaat ctatccaaag acagtcgttt
    30901 tgcttttgag tcagaagcag gaaaaaatac aggattttta gctaaatgga ctgactttta
    30961 ttattcctat ggtttaattg caggttacgg tggctatgta tttggtgatg atggcacaaa
    31021 tccttcagat atcggtttga ataatgcagg tgctgtagaa ggaatctctt atgcaacaga
    31081 ttggttcaaa aatgtttggc caaagggaat gcaagacata aaaagtgcag gagacttcgc
    31141 cagccagcaa ttcatgtcca ataaaactgc agcaatcatt gacggtccat ggcaagcgca
    31201 aacatacaaa gaaaacaata tcaattatgg tgtagccaaa attcctactc taaataatgg
    31261 acagccttat caaccgtttg gcggaggaaa aggatgggta gtcagcaatt atgctaaaaa
    31321 caaagacctc tctcaaaaat ggcttgatta tgtaacgaat caggaaaatc aagaaaaatt
    31381 ctttgaaatg gtaaatgaaa tccctgcaaa ccaacaagct agggaaacag caaaggctaa
    31441 aaacgatgaa ttaacaacag ctgtgattga acaatatgaa acagctcaag cgatgccaaa
    31501 catcccagaa atgggcgaag tttggacagg tgcagaaaac ttgatgttcg atgcagcatc
    31561 aggttcaaaa acaccaaaag aatccgcaga tgaagcagta aaaacaatca gtgaagccat
    31621 agaacaaaaa tactgattaa aatgataacc gtccattttt ataaaaaatg gaacaggtgc
    31681 ttcagataaa agtggcaaga gcgagatgga aaattatctt gctcttgttg ccattatctt
    31741 aaaagcagtt tgcggtttag caagtttaga gggggcattc agatgcagca agataaaaca
    31801 agcaatatca acgaaaaaaa aatcacgctt tgttcactta ttccagggat ggggcagttt
    31861 gtaaatggcc aagcttttaa aggtatcgtt tttttcgcca tattcgtcct attcattggt
    31921 cagatgcttc tgggcggatt tcatgcattg gtaggattgg ttactttggg aagtatacca
    31981 atggaagatc attcattatt tattttgatt gaaggcactt tgcaattcat cattacaatc
    32041 attttcttga ttttttacgg tttgaatctt ttagacgctt atcgagtagc gaagatgcgt
    32101 caagagggca aaaaaatcaa tcggacgatg aaggaagtca tacataatat aggtgatgaa
    32161 ggttttcctt atctattgac acttcccgct tatcttctca tgatttttac gatcattttc
    32221 ccagtcctag ttacattgtt cactgcattt actaattatg acttcaatca tattccacca
    32281 gccagtttaa ttgattgggt aggggctaaa aactttttta gtatcttctt tttaagctca
    32341 tatcgtaata ctttttttgc agtcttttcg tggaccgtga tttggacttt atgtgcaaca
    32401 actttgcaaa tcacccttgg tattctcaca gcggttgttg ctcaccagaa atttattaaa
    32461 ttcaaacgta ttttcggtat cattttctta ttaccatggg cggtcccagc ttttattact
    32521 atcatgagtt tttcgaatat attcaacgat agcgtaggtg ccatcaatac tcaagtgatt
    32581 cctttcataa accagcttcc ttttgtagat atcagtgcaa tctcttggaa aacggacccg
    32641 aactggacga aagtagcaat tatcttagtg cagggctggt taggatttcc ctatatctat
    32701 gtgatggtta caggaatctt acaatcgatt tcagaagact tatatgaagc agcaaaaatc
    32761 gacggagcaa atgcatggca gcgcttttca aacatcacgc ttcctgctgt cttcctagtg
    32821 gcagcgccaa catttattac acagtatacg gggaatttca ataatttctc catgatctat
    32881 ctgtttaaca atggtggtcc cggaagcgta ggcggaggag ctggttctac tgatattctt
    32941 atttcctgga tctataaact tacgactgga acttctccgc agtattctat ggcttcagca
    33001 atcacgttga ttatctccgt catcgttatc tgtgtgtcat tgctggtatt taaaaagaca
    33061 aatgcgttca atatggggga ttaacaatga aaaaatttca ttcgagtgct cgattccaaa
    33121 aacgactttc tcactttttt acttactttt ttcttttaat actgtcagtc attatcatct
    33181 atccgctttt gatcacagca agtactgcct tcaagtcagg aaatattgca gcttttagtt
    33241 tagatctttc aagtgactgg actttaggaa actttactcg gctattcagt gaaacacttt
    33301 acgggacttg gtataaaaat acattgatca tttctgtcct aacaatgatc gtacaagtga
    33361 ctgtagtaac tttggcgggc tatacttata gtcgctatcg ttttatcggc cgcaaatcaa
    33421 gtttgatgtt ctttttagtg attcagatgg taccgactat ggcagcattg actgcctttt
    33481 atgtcatggc tttcttatta gaagcgctcg ataagtactg gttcttgaca atgatctata
    33541 taggcggtgg aattccaatg aatacttggc tgatgaaagg atactttgac actgtaccaa
    33601 tagatttaga tgaatctgct aaacttgatg gggcagggca tttcaggatt tttttccaga
    33661 ttgtattacc gcttgtcaaa ccgatgctcg ccgttcaagc attatgggct tttatgggac
    33721 cttttgggga tttcatgtta gctaagttcc tgcttcgaac acctgaaaat cagactgttg
    33781 ctgtcggctt acaaacattt atttctgatg tgacaaacca aagagtctct ctttttgctg
    33841 ctggggcaat tttgattgca ctaccgattt gtctattatt cttcttttta caaaagaact
    33901 ttgttaccgg cctgacatct ggcggaacaa aaggatagga gcagagtatc atgcaaacat
    33961 ttccgttaaa ctatttttcc gcattacgta cgccaactca agtgtttgcg ggacgcaaac
    34021 tattatcatg gcctaaattt ttcttgatat ttgtattttt agtttccttg atggtcatgc
    34081 ctgtgactct tttttatgcc aatcagatcc aagcgattcc attagaacaa tttctatccg
    34141 ttcattcgct gatcgatgaa caaggaaccc aaaaattttc tgaacttgaa ttatctgaaa
    34201 ctgggttaca agcatcgcaa caaacgatag ctgtgacccc tgaaattttg gtaggtgtgt
    34261 ctttatctga gaaacaacaa tccgatcatg gaacttttat tgattttgag aaggaacaat
    34321 gggtgatcca acaaaaggat aaatctggta tacgccgcta tacgatgaat tatagcccat
    34381 ctttccaacc agattctgta cgtacgcccg aagatttcca acgattttta gaaagagaat
    34441 tttatgccag taatcgtcca acaatcattt tatcttatag tttaagtctt ggtcttgtcc
    34501 tatttgtcat gacgagtctc attttattcg gggcttcctt tttcttgtgg atgacaagaa
    34561 aaagccaact ttcatccatt catacattca aagagagtgc aaatttgatg ctgaatgtta
    34621 tgggaatagg ttccatggca gcagcagttg tagggtttat ccactttgac tttatcttga
    34681 tgctaagtgt ccaaacaacg ataactgtct tgctgctttt atgggttttt gtcaaaacaa
    34741 gattcaaaga taagcgtgtc gaatgacaag tttaagcgat ttatgcgaca atgaatgtga
    34801 aaaaaatgaa aaataaagta gtttttgaaa aatttctgaa aaaattaaac ttcttagttg
    34861 attcttttct tttttagtat taacgtaata gggtagtttt tttaaggagg aaaatcatgt
    34921 tagagaaaac tttatacaat caattatttt cacgttcttt ctcgctccct gtcgaagtaa
    34981 attattggga cggtacaacg aaacaatacg gagatacgga cactcctccc caaatcaaaa
    35041 tcaatatcca tgaagaaatc ccaatgaaag agattacaag taacgcttct ttggcactag
    35101 gagaagccta tatggatcat cgaatcgaga tcgagggaag tatccaagca ttgatcaacg
    35161 atgcttatca gcaagcaaac agctttttac gaggaaaaga ttatctaaaa tggctgccaa
    35221 agaaagaaaa acatacgaaa caaaaaaaca aagaagatat ccatgcgcat tatgacttag
    35281 gtaacgactt ttataaactt tggctagatc caactttgac ttattcttgt gcttatttca
    35341 atacacctga agatacgttg gaacaagcac aaatcaataa agtccatcat atcttagaca
    35401 aactctttat caaagaaggc gaaacgttgc ttgatatcgg ttgtggctgg ggaacattga
    35461 tgttcactgc taccaaagag tataatgtca aggcaactgg gatcacacta agtgaagaac
    35521 aatacgattt gattaccaaa agaataaaag aagaacattt agaagataaa tgccgtgtat
    35581 tattaatgga ttaccgcgaa ctaaaaggcg aaacgtttga tcatatcaca agtgtaggta
    35641 tgttcgaaca tgtaggttct gaaaatcttg aaggttattt caaagtggta aaagatttat
    35701 tgaaaccaaa aggaacagct ttgatccatg gaatcagccg acaacaaggc ggtgcgacaa
    35761 atgcttggat caataaatat attttccctg gcggttatgt ccctggtgtt actgaattag
    35821 ttgataatat tacaaaaaac gatttgcaac tgattgattt ggaaagctta cgtcgtgact
    35881 accaattgac attagaacat tggacaaaga attttcatca agtccaagat caagtcacga
    35941 aagaaatggg tgattccttc tatcgaatgt gggatctata tttacaagct tgtgcagctt
    36001 catttgaatc aagtaatatc gatgtgattc aatatttgct tgttcaacct ggaaacaatg
    36061 acatcccaat gcatcgtgca taattgaggc aaaagtactg tttaaagact atttaaaaag
    36121 aagctaagtt ttatgatgct ccagaagtga acttttttgt ctaacttcta ggattgtctc
    36181 taaagcttag tttctttttc tttatatttt cataataaaa gtttggttct ttaattttta
    36241 aacatgacag aaaatttttt cttcgttata cttgtaatag aatactattt tataacaaag
    36301 gggatcgaat caatgaaaaa aactgttatt tatactagtt tatttgcagc tttgttttta
    36361 gctgcttgtc aatcaaatac caatcaaggc acatctggga atacgtcttc cagtagcgaa
    36421 cagcgctctt cacaggtatc ggactcctca acaaaagcaa gcacatcttc gtctagccag
    36481 acaaaaagtt ctagtgaagc aagtactcag tcagatatca aagtgtctgc agctcgtgcg
    36541 atcgaattgt tccaagataa atacccagat actgcaatta ctagtttgga attagatagt
    36601 gattggggat cttacttcta caagatagaa ggtgtcgatg atcaaaaaga atacgaagca
    36661 agaattgatg ctcaaaatga aaaaatggag acagagaatc cagaaactct agatcgagat
    36721 gaacaaaatg gtcaaaagaa aaaagaagat ggtctggatg ttgataaatt gatcacgatt
    36781 gaggaagcag caaaaatcgc cgtcgacaaa gtaggaagcg gtacggcaac agattgggat
    36841 ttggataaag atttaggtac tacttattgg gaagtcaaag taaaagacgg caatcaaagt
    36901 acatcagtca agatcaatag ccaaacaggc gaagtactgt caactgaaca agatgattaa
    36961 gaatttcttc ggaaaacaag agttgatttt tcttttagtc atgatacact agacaaaata
    37021 acttgtttga aggagcgatt tacttgagtt tagaaacgac ctttgccaat catatctttg
    37081 ccaatccttt gatgaatgca tctggtgtcc actgcatgac tacccaggaa ttagatgaac
    37141 tggctcattc agaagcagga gcatttatca ctaagagctg tacgatcaat gaacgaaaag
    37201 gaaatcccga accacgctat tttgatgttc ctttagggag tatcaattct atgggacttc
    37261 caaacttagg tttttcttat tatctagagt atgctcttgc gtatgaaaaa gtgcaggaaa
    37321 accaaaatca accgttattt ttttccattg ccgggatgag tgtccaggaa aacttggaaa
    37381 tgcttgagaa aatagaaaaa agtgggttta atgggatcac tgaattaaat ttatcttgtc
    37441 ccaatgtgcc tggaaaacca caactagctt acgattttga ggccacttat gaaacactga
    37501 aagaagtatt cagcatcttt tctaagccgc taggaatcaa attgcctcct tattttgatt
    37561 ttgctcattt tgatcagatg gctgatatct tgaatcagtt tccattgacc tatgtcaatg
    37621 ctatcaacag tgtgggcaat ggtttgtata tcgatacaga acaagaagca gtagtcatca
    37681 aaccaaaaga agggttcggc ggcattggcg gagagtatat caagccgact gctttggcga
    37741 atgttcgcgc tttttataca cgtttgaaac cagaaatcca aatcattggt acaggtggga
    37801 ttcggacagg acaagatgca tttgaacatc tgctatgtgg cgcaagcatg ttacagattg
    37861 gtacagagct tcataaagaa gggccggaaa ttttttctag aatcataaaa gaattaacgc
    37921 agattatgtc agaaaaagga tatacgtcaa ttgacgaatt taaaggcaaa ttacgcacca
    37981 tttcatagca aagagacgaa ggtcatgatt gatcttcgtc tttttttata aaaaacactc
    38041 gtctgttcat tcctgtgcaa gcgtactcat tgtatgttag acgaacttgt gggaaaataa
    38101 cagaggcaaa aaggaggata atatgaatga tgcaacagaa ttagcaaaag agcttaaaga
    38161 aaaagaattt agttttcaag aactagttac ggatatacag aaaaaaataa agaagcaaaa
    38221 tcatgaattg aatgcgtttg tcgcgcttga accattcgag agtaattccc acgagctatt
    38281 gattccccag tcagaaaaag ggccattggc tggtatccca tttcctttaa aaatgctcgg
    38341 tcaagaaaaa aagggatggc tagctacttc aggatctcgt ttatttgaaa cacaccgagc
    38401 ttctcataca tctaattatg tgcggcaagc agaagcgata ggtctagttc cttttggaca
    38461 aaccaatgcg ccagagtttg gtttcaaaaa tattacagac ccagtcatct atggcccagc
    38521 aagaaaccct tggaaccttg aatgtacacc gggcggatca agtggaggag ctgctgctgc
    38581 agtttcatca ggaatcgttc ctttagctgg tgctagtgat ggcggtggtt ctattagaat
    38641 acccgcttcg ttttgcggat tgattgggct gaaaccttcg cgagggacga tgccagttgg
    38701 tccatatgct tggagaggat ggcaaggagc tgctattgat ttcggcttga cagtttctat
    38761 gagagacacg gaggctttat tttatgggat gagaagtatc cattccggtg cgccatatca
    38821 agtatcgcca gttgagtggc aaacacatcc aagaaaaaaa cggttgaaga tagctttttg
    38881 tgttgattca ccgatccact cacagattag tgaagaggca gtacttgctg taaaaaaagc
    38941 tgtagatttc cttggaaaag aagggcatga tttgacagag attccttacc cactagatgg
    39001 tcgtaaactg atcacgtctt attatcagat gaatggcgca gaaacagcag caatgatccg
    39061 ctcgattgaa acaggcatcc agcgagctgt cagaaaagaa gaacttgaac ccatttcttg
    39121 gactttgttc caatatggtc agaagctctc tgccgctact tacatcgatt ctctgcatgt
    39181 atgggatgaa gcagcaattg tcatggaaaa cctacatcaa gaatacgatc tattcctttc
    39241 accaacgact gcatttaccg cccctaaaat cagcgaagat ttgcaaagtg atgcgataag
    39301 agcacagatg gaacacatca gtgattgttc tgaagtagaa ggtttagacg ttgtttcttc
    39361 catgtttgag gagagcttga agatcacacc ttatacacag ttggctaatc tgactggaca
    39421 accagcaatc agcttgccta ctcatttgac aaaagaaaac atgcctctag gaatccaatt
    39481 tatggctgcc agaggaagag aagatttgct atttcaaatc ggctatctat ttgaagcaaa
    39541 tcatcagttc catttaccaa atccatggaa atagcagaaa caaggagaag aagaatgaag
    39601 aaaaaaagat tgtttgctct aataactagt gcctttttta ttttaagcgc ttgcggtaca
    39661 gctgccgcta atcaggaaga taacagctgg agtaatgtag aaaaaaagaa agaattagta
    39721 gtagcgactt ctggcacact gtttcccgca tcttattata atgatcaaaa tgaattagtt
    39781 ggttatgatg tagatttggc aaaagaggtg gctaaacggt taaatgtcaa aatctccttt
    39841 aaagaataca acgtggatgg acaaatcact tctgtttcaa aaagagaatc agattttgct
    39901 gcgaacgatt ttggtatcac aaaagaaaga gaaaaaaaat tctccctttc agagactttc
    39961 aaatattcat ttgacagtat gatcgtacga aaaaaagatg attcaggaat ccactcactt
    40021 gaagatctta aagggaaaaa agcagctggc gaacccaata ctagctatat gcgtttagcg
    40081 gaaaaacacg gagctaaatt agtcacatat gacaatgcca cgaatgatca gtatttaagc
    40141 gatgtagcaa atggtcgaac agatgttatt ctaaatgatt attatctgca gaaaatggcc
    40201 gtatctgctt taccagatat ccctgtaaaa atactggaga acgtttattt caatgaaaac
    40261 gaaagtggtt ttttattgaa agaaggaaat gatgctctga aaaagaaaat cgatggcgta
    40321 ttaaaagaga tgaaagaaga tgggacgaca aagaaaatat ctgaaaaata ttttcaaact
    40381 gatgtttcag tcaaaccgaa agaaacgatg gctgagtcgg tgagtgagtg atgtactttc
    40441 ccctaattga tttttccctc atgtggactt cacttccttt cgtactgcaa ggcttggtat
    40501 acacattagg gattggcttc gtgtcgtttc ttttaggaaa tttggtaggg ttgcttttga
    40561 cagttttagg tcttttggat tggctgcctc tgaatgtatt tattcgtttt tatttatcgt
    40621 tctttagagg cattcccgca ctagtcctgc tgtttctgtt atatttcggg ttaccttatc
    40681 aattaagtgc cttaacggct tcagtcatct gctttacgat taccagcagt gcatttattg
    40741 gtgaaatcta tcgaggatca ctagcaggcg tttcttcagg tcagtgggac gctggtttag
    40801 cttctggttt ttctttcttt ggggtattga aatatatcgt tttgccgcaa gcttttcgaa
    40861 tctcagtacc ggcactcagt aacgtagcta tggatctagt gaaaggtact tcgttagcag
    40921 caacaatcac agtgccagaa atatttcaaa aagcaaagat cataggcggt cggacattcg
    40981 attatttttc tctctatatt ttagttgcac tgatttattg ggtcatttgt atcgggatcg
    41041 aaaaagtgca acagtattta gaaaaacgtt ctatccatca gtcaatctga aaaatagaaa
    41101 tggtgctagc gtggctggca ccatttctat tttttgtttc ttgtatattc tgcctagtat
    41161 tttaaaacca tttcatgtaa aatgtaaatc gaaaatatgt tcggaggtag aaaacatgaa
    41221 gaccccgcta accatcgaaa tagaatcctc gttataccaa tattgcatcg agcaaggcgg
    41281 cttggttgta gaagaagtca cgatgcctga tgaccaagga attgtagata cgcttagctg
    41341 cttgacaaaa tttgatggaa gccgagaatg gcgatgctat gagctgaaag tgacaaaagc
    41401 agattttcgc tctacagcca aactgtcatt tgttggacat tataattatt ttgttttacc
    41461 aagtaaacta tatgaagaag taaaaactga aatccctaca ggaataggtg ttttagtgta
    41521 tcgtccgtac acggttgaag aggagatgcc tgcctctggt acatttctga ttgcgaaaaa
    41581 agcagcaaag aaagagcttg ctgtaccgga agaagctttg acaaaccgct tcatggcttc
    41641 tttattcagg gaagtaagaa aagcaaagca aatggagcga ggcgtaaaat attttccaaa
    41701 agatcaattg tacaaagaat tgaaaagacg ttcagaaaat caagatccat tttctaacga
    41761 acgatattat gaacggtttt tagaggaaat agctagtcag gcaatcaatg agttgcaaga
    41821 agaagttatc gcgcttcagc atgattatga atatttgagg caacaacagc agatccgtcg
    41881 gttgcctaca gagccattgg agtgaaaaat atgtactatc cttatttaag aggaaaacaa
    41941 tttgacctat tagcattaaa agaagcactg tcgcgaggat tgctctccaa taaaatccaa
    42001 ccagtgatcg aaccagtacg tgattccgct acgcttaaaa atgtgatcga attatttcaa
    42061 aaaaagcacc atccaatcgc tgtgatccaa aatccccaag taggacaatt caaattattc
    42121 gatcagcatg tgcacagttg gacagtcaaa gaaaatagct ctgtcgtccc tgcacagatc
    42181 ttaacgccag aaaatctcag tgaagtactg gattcacctc cagcgtttct tgttttcgat
    42241 ggacagcact acccaaaaga tacagaggtg tggaaacagt tggcaggagt tgattcgaaa
    42301 tttttgatac cagatactag tcgctttcgc atctggctgc ccgaaaacaa aattgtgatc
    42361 cgtgactctt ttcaaacgag aaagcatgtt gaaagttatg cagataaaac agatgacttt
    42421 tttagtgatg actatctttt tttccacgca gacggatata ttggattttc tgatttcacg
    42481 atcgaaggta gccgttattt cgataaaggg ttccctagcc gagcgcttgc tatccatcta
    42541 acgtatattg atgcttacgg caatatacgc gtgaaacatt ttgtctctga cagtaacgac
    42601 tctgctaaag accaagcatt gaagtttctt gaagcaggag ataaaatgaa aaaatggatc
    42661 atgcgtcacc atcatcaact cttgattact tcaggaatga ttgaattatt ggctctttac
    42721 cagcaacaaa aattccctgg actgggagtg ttgaaaaagt ggtcacttat gcatcatttg
    42781 gaacttatta gtcacctgct agatcatcca aaagattggc ttaaatatta ttcaaaagtt
    42841 caggaactat atgaagtgat ccaatgatta gataatgtaa atgaaaaagg aatagaaaag
    42901 aggaaactgg atgtatattc ggcaaacaag gaaagaagaa gtagaagaag tggtagaaat
    42961 cattaatgcc gcagcggatt tcattgcttc gcaaggctct cctcaatggc aaaacgggca
    43021 gaaaccaaca aaagaaacga ttttgaacga tattgaaaga aaagaaagct atgtattaat
    43081 agtggaagat caaattgctg gaacagctgc attagtctca ggcgtggatc ccgtttacac
    43141 aaaaatagat ggtagttgga aagaatcaac tggtccttat ttatcgattc atcgtgtcgc
    43201 tttagcagaa agtactcgag gaaaaaaact agcaaaaccg ttcttagaac atttgttgac
    43261 gattgcagcc agcaaaaata tccaagatat ccgtatcgat acttatccag ctaatgaacc
    43321 gatggaaaag accatctatt ctgctggatt cacttatcaa gggatgattg aatttccttt
    43381 tgaacacggc gaaagaaaag catatcaaat aatcatcaat tgactataaa atcatctgag
    43441 caaagataaa gacattaaca ctactgaaaa aaacaatcat atgatagcca agaggttgta
    43501 acagaagtgg ccagcttcaa aaaacaagaa agaatttccg aaaatgcttc tcaaattttt
    43561 gtggaatttt agcgtatttt acgaaatagc tgctttctga aactcccttc atcgagaggt
    43621 cgtgacatag cttttgtcac gatctctcgg cttatcctac gagtattatt cgatacattt
    43681 atatcaataa tccttttttt attttatctg cagcttcttg gaacgctcca tcgtttgaca
    43741 cgactaaaag tgaggggata gctttcaaac gtttcaactg tgctaaatct tctacataaa
    43801 ccgccagtct ggctgaaatc tgttcagatg aatctccacg ttttttcagg cgcgattgca
    43861 atgtttcttt agagatcgta ataaagatag gacggactac cgtcccgaaa tactcatata
    43921 aatgccaaaa accatttgct gtcaaaacgg cgtaacaatt tcctactgtc agtttctttt
    43981 taatttctgc tttactggaa ccatatttat gttgagcata ttggtcccac tctgcaaact
    44041 catttccctt gatcatctga ttgaatgtat tgtcagaaac aaaataataa tcctcttgat
    44101 ctttttcccc aggtcttggt gatcgattcg tgtaagtaat gatcttttgt tccggctgaa
    44161 aaatagcttc tgcgagtgaa gtttttccag aaccagaagg accgataaat acatagcaat
    44221 aattcattgt catttctcct ttgatgattg aacaagtgaa aaagctttgg aaaatatttc
    44281 caaagctttt aaaagtaact ttgctttttt gtattttata ccagcaagcc agtcacaata
    44341 gcacttaaaa agctgactaa agtcgctgtt agcaaaattt tcaatccgtg ttttgcgaca
    44401 agatttcctt tttcttcatt taatcccttc atcgcaccag aaatgatccc gatagaagag
    44461 aagttggcaa atgaaacgag gaagacagat ataattgctg ttgtacgctc agtaaaatgg
    44521 aaattaccat ttgctaattc tgtcatagca acaaattcat ttgtaaccag tttagtcgcc
    44581 atgatactac cggcatccac tgcttctttc catgggattc cgctgatgaa ggcaagaggg
    44641 gcaaagatgt aacctaggat ctcttggaaa gtgatgccga atatcccgtg gaagatagca
    44701 ttgatcatgg caatcaaagc aacaaaaccg ataagcatag cagcaaccgt gatcgccaca
    44761 tggaaaccat caagaatgta ttctcccaac atttggaaaa atgtctgttc tttgttttct
    44821 tcaatgatca gctcatcttc tttttcttct aatacatagg gatttacaat tgaagccaga
    44881 atataaccgc caaataagtt caaaacaagg gcagtgatca catatttaga atcgatcagt
    44941 gccatataag atccgacgat cgacatagaa actgtagaca tagccgatac actcatggtg
    45001 aacaaacgtt tctcagatag gaagggtaat tcctttttta tggaaataaa aacttccgat
    45061 tgtcctaaaa tagcagaagc tactccatta taagattcca atttgcccat cccgttgatt
    45121 ttacttaatc ctagaccaac agctttcata aataatggaa gaatcttgat atagcgtaga
    45181 ataccgataa tagcagaaat aaatacaatt ggcatcaata cggaaagaaa gaaaggtgca
    45241 cttcctttat ttgctacacc ggcaaaaaca aagtcaacac cttgcccagc aaaagcaaga
    45301 agatgatcaa acatttttgc caatacagaa acaaccgctg taccaaaagt tgtacgtaat
    45361 aatacgaaac caagaacaag ctgtaaaacg aacatcacaa ttagtggttt caatttgatt
    45421 gcttttttgt tactgctgat caaaaaagca agccctaatg tgacaaataa accaataata
    45481 cctaataaat aactcaaata atcactccta ttccaaattc cgttttattt tacctgtttt
    45541 gcaaataaaa tactaatctt tagtgaaatt ttcattactt ttttagagtg aaacgcattc
    45601 attttccatt agaaaataaa gcgaacaaaa taaaaggttt aaaaagtgta acgtcttagt
    45661 aaagtctata aaaaaacgaa actatcgatt aagctaatag tttcgttttc aacatttata
    45721 agtaaacttt ttctattggt aatatagatt ttcggaaggg tagacaggtg cgcaggtaat
    45781 atcgattcct agttttttca atgtttgttc atcattttta ttgaccatca cagtagagtg
    45841 ggcttgtgct ccctcaagtt cagctagttt atcataagca acttgagcga ttggattcgt
    45901 tactgcactg attgataaag cgatcaagac ttcattgatg cttagtgcat ccaaagagga
    45961 atgaagtgtc tttgatttca atccttgtat tgtttcaaga actaatggtg acaataaagg
    46021 aatgtcatct gcgatatggg ctaaatgttt tactgcattt aagataactg ccgctgacgc
    46081 atccatcaat gaacttgtac gcccggtaat gatgacttca tctggtaatt cgattgcaat
    46141 cacagcagaa ggatcttttg taggactttg cgcttgaatc ttatctgcat agtcatgggc
    46201 agggactacc acgtttcggt cttcaggacg taaaccgact tcttccataa tcagtttgat
    46261 tcgattaact gcttcttcgt cgatcaatcc ttttttgaag tcgcattctg tttggaaata
    46321 acgacggatg atttcttgtt tagaggcttc ttggacaacg gtatcatcca cgatcccaaa
    46381 tcctacacga ttcacaccca tgtcagtagg agattgataa acagattctt taccagtaat
    46441 tttttcaata atacgtttga ttacaggaaa tgtctcaaca tcccgattat agttcacagc
    46501 tactttattg taggcatcaa agtggaagga atcgatcata ttgacatctt ttagatcgac
    46561 tgtggctgct tcataagcaa tgttcagcgg atgtttcaac ggaacattcc atactgggaa
    46621 agtttcgaac ttggagtaac cagctgcatg tcctttttgg ctttcatgat acagttggtt
    46681 caagcaagta gcgagctttc cacttcctgg gccaggagct gtcactacaa cgattggttt
    46741 agttgtttca atatactcat tttttccaaa accatcttct gagacgattt tttctacgtt
    46801 gactggatag tcttcgattt ctgagtgttt atagaccttg atgtttcgtc tttctagttt
    46861 attgataaag actttcgtgg caggctgtcc actatagcga gtgataacga cactgttgat
    46921 taataagcca taaccacgta attcatcgat taaccgcaag atatccatat cataagtaat
    46981 cccataatca ccacggatct tattacgttc aatatctcca gcatagacgc aaatcagtat
    47041 ttcagcttga tctttcaatt tttgcagcaa tttgattttc gcatcttctt caaatcctgg
    47101 aagtacacgt ttcgcatgct tgtcgtcaac taacttccca ccaaattcta aatataattt
    47161 gtcatagtgg ttcactcgtt ccaaaatata cgctgactgc tcttcaatat atttttctga
    47221 atcaaaacca atttttttca ctaggttccc tccttaagta ctaaagcatt ttttcatttt
    47281 tttgaggatt cgtcaactgg aagagcgaaa ttcctcaaaa aaacaaagaa aatggtttaa
    47341 accaacttgt ctagatagtt attcattggt atgatagttt ttgataagga aggtgctaca
    47401 tgaaaaaaag acagaaaaag aaaaacgcat ataaacacta tatccgttcg atttttaccg
    47461 gttatgaaag aatgcttgaa gatcccgaat tggaacaatt gacgtttact tatctaaacg
    47521 aagaaacgca gctaactcgt gatgagcatc agcgcattca tttcacaaca agagatctgc
    47581 ctagtaaata aaaaataaga taaaggtcat cggattcgat tctgtcctat cagatggaaa
    47641 gactagttga tcgaaaaaaa ggctgtacca aattttggtg cagcctgttt tatggagcat
    47701 tgcccgctct tttttataac aattttttac agaaaatggt actattgaag tattaataag
    47761 tgaaaaacag atgagtaaga ggaggcaagt agtatgaaag agccagatac aaatgcaaat
    47821 tatgagccag acgaactaga ggagacgaac gcggatcagg atacaggaca agaatcgtct
    47881 tctttttacg atatcaaaga aaaagcaagc atgaatcttt cccaattatt agaagactta
    47941 caggcttttg aaaaagctgt ccgcgaagaa gatatccctg agatctaccg tttatacaat
    48001 ggccgattga atgaagaatt aaaaagaagc tcaaatgaaa atcacgagat cgatcaattg
    48061 ctgatgcgta agatccatga tcgtttttta caggaatttc cttttatgga acaagtagaa
    48121 aacatctcgc caacgatgag ctattacaaa atcggcactt attaccatga tcggcccacg
    48181 gtcggtattg atgcaagtct gccagaaatt tttgttttgc ctaagatcga tgaagagtgg
    48241 gagaaatatt cccaaccgga gtcaacggac tatgacaaaa aaatcaatga gctagacgca
    48301 aaggtgatca ctgctcagac agaaattgaa cgtttagatg aacaaataaa agaaatcaat
    48361 cgacaaatga ctgatttgaa cgacaataaa ggattcttga atcgtaaaaa aatcgaggaa
    48421 gaaattcaag aattagagaa gaagaagcaa gtactatcta atgaaaaatt aggctggctt
    48481 ccttatattg agacgcctga aacaatccag cagcaaaaag aagcattgaa gcaagaagca
    48541 agagcagacc agcttcgtgc agccatcgta gaaaaagaac aaaggcaaat cagacgctat
    48601 ttcggtagca aagagggatt cggtcaagca atccatgaat tcttgatgaa ttatcttggc
    48661 aatgagaatc ctaataatca atctaatgaa ggaggttccg agtatgaatg aacacgatga
    48721 agcagaatgg acaaaaaatg aagaacaaac agaagatcat gaagaaacga acacttcatt
    48781 ggaagaacaa acaagtaatc ctctggatga cacagaagaa atccctatgc tagaggaaac
    48841 aaaaatacat gaaaaagaaa aagtaactcc gacgaatacg aatactgcag aaaacaccca
    48901 tgcttacgaa ataactgaaa atcccgggta tttagaagga aaagagcaag atcatcaaga
    48961 tatcgtcagt ttgattgaaa cagcggaagc agaattagcg gatttacgta tgagaaaaga
    49021 tttgatcgaa tcagattttc cacaattatt agaatcatat gatcgtttta tattgaatga
    49081 agaaccaatc tccacgatcg gaaagacaag cctgatcgaa tacttcacgt atgaattact
    49141 gaaaccgttg aatgatattg gtctggaacg gtcaaattca gcttatgaca catggaaaac
    49201 aagacatttt tcgatcaatt atacgttgaa tggacaaaac gaattagtct tttcctttgt
    49261 attaccgacc atcgatcaac gataccaagt ggaatcgatg agtctcctaa acgtatcacc
    49321 agagacaatg gaaatcaatg tccaagatga ccgggtgctt tctttgatcc gacactggtc
    49381 agtggatcga gtattctctg cagggcagat cacgatattc aatcacaaat tgaatcaatt
    49441 attagcccat gcaagaacac ttggttttac cgttaatcag acattactag acaatacgaa
    49501 gccacttcgt ttgaatcttc agagcgaatt tgaattgacg aatgaagtat tggatgatat
    49561 ttttatcgtg gcaatgaaaa atccatccta cgatatggaa aagatcaacg aaactactta
    49621 tcaagtacga ttagataaag gacagtcttt aacgataaca acaaacgaaa aagatcaaac
    49681 agtcttgtcg atctcatctg gagactaccc acgatctgtc attgatttct ttatcaatta
    49741 cgaattcttg gtcccattaa tggtgagaaa gtcctgattg gataagttgg attaccagat
    49801 gtcattcaga taattctgtt ccaaaggact tgcggcagtt atccagaata attgtcacaa
    49861 gtcttttctg tatgtactaa atggacgtga ataacttgct gatgaaggag ggatttcatt
    49921 aggaatgtcc acaaataaaa tagcacagct tagcttattg tctgctgctt gtgtagcagg
    49981 taggatcgct tttcaattta ttccgaattt ccagccaatg actgctattt ttttatttat
    50041 agtactttac ttaagcttaa aagatgcgtt aattgtcatg agtctttcta ttgcgatcag
    50101 cagtttttat tttggtgttg gaccatgggt catcggccaa tggatcagct atactgttgt
    50161 tctgagtttg ttttcaatcg tctgcttgag aaaaacagtg caaaggaatc tatggttaaa
    50221 gggagtctgt ttttttctgg cgggcatggt ttacggaata ggtatgaccg tttttgatac
    50281 gctgttatat cgtttgcctc aaccttggat ctattacctt caaggggtgt cgtttgattt
    50341 gatgcacagt ataggcaatg ttgtgttctt tcttgttttt ctgccagtcg tcaaacgttt
    50401 ttacaatcat tcaggaggaa aaaatgaaaa aaataattta tagtttgctt gcaatgagtg
    50461 tcagtatact cattttgaca agttgcagta caacctatac acaatcggct tccaaccaaa
    50521 aggaggaagt aaaaacatcg caaaaagcaa caatcacgtt acaagaaaat ggtcaaaact
    50581 tttcggaaaa agaagtggtc ttcaaaaaag gcgatgattt actgagtatt ttgaaacgaa
    50641 attttgaagt caaagaagat aacggcttca tcacttcgct tgaagggcac tcacaggatg
    50701 agaaaaatca actctattgg atgtttactg tggacgggaa aagtgctact actggtgcca
    50761 aagagatcaa attgaatgat ggacaatcgg tcattttcaa tttgtctaaa ctataagttt
    50821 cataaaaaac tgttaaaggg cgaatattgc cataattcag tgagttcagt tgatgaaaga
    50881 aggaaaataa catggaaaat tttaaaaaaa gcggaatgtt ttccgtaatt gctgctctag
    50941 gggctaatat tctagttgct atcagcaaat ttgtcggcta tgcaatcagt ggcagtgccg
    51001 ccatgctaaa cgaaagcatc cacagtatcg ttgattgcag caatcagatt ttgctgttat
    51061 tcggtgataa acaagccaat aaaggacaga gcgagttgca tcaatttggt gaaggacgag
    51121 caaaatactt tttcagtaca atcgtcgcaa tgatgctttt cttcggcggt ggggcacttg
    51181 gggtgatgga agcttttgaa aagttgacgc atccatccca tgaagtgggt aatccgatca
    51241 ttgttatagc aatactgtta tttggtttgg taatcgaagg aagttcgtta cgcgtggcaa
    51301 tgaaagaaat caaagaattg aatactgaaa aactttccac gatgcgcttt ttgcgcgaaa
    51361 gtcgacacag tgaaatactg attattttta cggaagactt gtgtgctgta atcggtttgg
    51421 tcttggcttt agcaggaaca atattgactt atgcaaccgg gattgctgca tttgatgcga
    51481 tcagcggatt gctgatcggt ttcttgctga tgggagccgc cattttccta gcaaaagaat
    51541 tttatagtct gatcatcggt gaaagcgtga cagcaagcga tttatcaaaa atcaagttag
    51601 cttttgaacg tcctgagatc agtcggctga tcgatataaa aacagttcat ttaagtccaa
    51661 cggagatttt agttgcagca aaagcagata tcgttggtaa caaagagaat gaagcttact
    51721 cggtcatcaa tgatattgaa aaaaatatgc gtcagtctct tcctgacaag aagatgtata
    51781 tatacattga aacagataaa tatgacccaa attattctcg taaataaaac gtttattcat
    51841 gaattgaagc aaaaatgaga aaaatataat cttgttttta aataaaacgg ctacaattta
    51901 gcgaatttat gttacaatga gtccgctgtt tatttgttgt aattaaagga gatattttaa
    51961 tgaacaaaaa gaaaagtgcg ccatggcttc aagctttgca tgcagtgatg ccactttgta
    52021 taagctatat accagtagga ttagcttgcg gagtattgtt gcaaaaagtt ggatttgatc
    52081 ctctcttatc aggactgtta tcgattctgg tcttttccgg tggcgcgcag tttttagttg
    52141 catctatgct tacgacgcaa gcatcatttg caacgacatt attgatggtt ttctttttag
    52201 aattacgcta tacattatta ggagcaagtc ttgccggttt tatgaaaaaa gaaaagcgga
    52261 cgtttttggc tgtcttttct caatcactga atgatgaaaa ctatgcggtg aactacttaa
    52321 agttttcaac tgacccttcc tggaataaaa gaaaggcttt atatgtcaat tggttctcaa
    52381 tgactgcatg ggccggtagc aatatgatcg gtaatttttt cggctcggtg attcgagtag
    52441 atgctgacct tgtccatttt gctttgacgg ccatgttcat tttcatgttt atcatgcaga
    52501 tgaaaagcgc actactgatc tttacaggtt tattctcagg cgtattaggg gtcgtattca
    52561 tggtattatt ccaaaatact ttcggattga ttgcagcaac agtcattgct tcatttgccg
    52621 gatttatgct ggagaaggca ttcaaaaaag aaaaagctaa gaaactaaga gaccgcggta
    52681 aagatgtcaa tgagccgctc ttcaacttcc cggaacagga ggccaatcac catgactaaa
    52741 ctatatttgc tacttttagt tgcttgtctg ttcatcgtgg catatatccc gcgcttgttc
    52801 ccgatgcttt attttactca tcgaaaagtg ccatcctggt tcagtgactg gatgaaatat
    52861 gtaccggtgg cattatttgc ggcgttagct tttaaagatg ttttcattac tcatgaacat
    52921 ctggatattg cttggaatat caaaatagct gcaatgattc tagtagcagg cgtggcttac
    52981 aaaacacgtt cgatggcgtt gtcagttttg acaggacttg cgtctgtctt tttgctgtct
    53041 atgctttaaa ctgttttatt ttattaaaaa cagagattgc gacataagtt gttcaaactg
    53101 atctatccga actgcatttg caacagtcaa ttctggatgc aagtttattc gtagggataa
    53161 aaaagattag tgttggctta tgatgcaagc tcttttttat tttaggaatt agagagggat
    53221 ttgtccttct atgatttttt gctataatag ggaagatgtt tattttatga aagaatagtt
    53281 gagggaattt aaatgaataa aaaattgatg caaggaacat tttggctaac ctttgctaat
    53341 cttctatgta aagttttagg ggtagtctat ttgattccct ggctaagtat gatggggaac
    53401 aatcaagatg gtatgcttgc tacgacattg tataatgttg gctatcttcc ttatggattg
    53461 ttcctgatgc ttggtacagt cggatttcca aatgcgattg caaaaaaagt agctgtagcc
    53521 acaaaagaag gagataacgc tgcttgtcgg ctgattttta gaagtacgat caatatcatg
    53581 tttgtcatcg gtatcgcctc tgctgcactg atgtatttat ttgctccttt actagccgga
    53641 atcagtccga ttgctaacgt taataatggg atactagcta tcagaagttt atgcccatct
    53701 ttgattgcta tcccaattct tagtgcaatg agaggatatt tccaaggaaa aaatgatctg
    53761 cggccttatg gaacttcttt gattatcgaa caggctgtgc gagtgatcgt tatcttggct
    53821 ggtacttatt acttgcgcgt attgactgac ggaaccatat tagaagctgt attgatcagt
    53881 acagtcgctt ctttcttcgg tgggttagct gcgattatcc atatgtatat cgtcggacgc
    53941 agaaaagact tcttcgaact taaagatttt ttgatttcca gccgttattt cgaaagagaa
    54001 aaccgatctg cctctgtctc tatcattcgg gaaacactac cgtttatttt tgtcggctca
    54061 gtcattacta tcgttcaaat gatcgaccaa gtaacgatga aaccactgct tcactttttc
    54121 cgtccagaga tcgcagatca gcaactggaa ttcttattca gccgtgcttc tgtcaatcca
    54181 aataaattga cattgatcct gatctccatg gtaggaacag tagctataag cagcttgcca
    54241 atattaagca cgatgaagaa aaaagatcgg atacaaatcg aaaaaacagt aggagatagt
    54301 ttttcgattg cgttattgat tcttcttcct tcattgactg gaatgtcttt attagctgga
    54361 ccactataca cattgttttt tggctatgat cctgaaagtg tcggatattt ccaaatggct
    54421 ttgctagctt ctttgttttt ctctttattc acgatcctat ctacgatgct tcaatctttg
    54481 aatcaccatc gttttgcgat ccgattaact gtagaagcaa ttatcctgaa agtcatcttc
    54541 caagtaatcg gattaggatt agttggcgga tacgggatga gcttatcaaa tggcttagca
    54601 ttcggtattg tctttgttcg cggctatcac tttatgtgca aagaataccg aatagcacct
    54661 ttagcaaaga tcagcaattt tttcttaaaa actttccgct ccaccctgat tatgcttgca
    54721 gtatgttttg ccgttttctt tttgctgaat cagcgattgt caatggaatc aaaaagctat
    54781 gcaatgattt attgtgtggc tgtaggaagt attggcggct tagtattttt attttctcaa
    54841 ttcggaaaaa atgggtttcg aatgttgaaa aatgttaaac atcatagcag aagcaaagaa
    54901 taggttaact aatcgttttt tgctgtcact tgaaaaacgg caactcgtat gattacgctt
    54961 gtcgtttttc tattttccaa ttttagtttc atatctaatc ttacttatga tatagttata
    55021 aggtctggaa aaattctttg aaacgaggaa attaaacaca tgaaacttgc agtagttaca
    55081 gatagttcgg catatctgcc ggaacgtatc aagaaccatc ccgacttatt cgttattcca
    55141 atccctttga ttatggatgg gaaaatttat aacgaaggaa tcgatatcga agcggatgaa
    55201 tattatggac ttttaaaaaa cagtaaagat tttcctacta cttcacaacc tgtcgtaggg
    55261 gaagttttgg gtttatatga ggaattgaaa gagaaagggt atgacacgat tttaagtatc
    55321 catctttctt cagggatttc tggctttgtc aacacattat ttgcgatgaa agatgacatc
    55381 agcggagtaa cggtcattcc ttatgactct aagatcacta gtatgccgat gggacacatg
    55441 gtagaagctg ccttagactt aaatgatgaa ggtcagtccc ttgatgaaat acttgcacat
    55501 atcgatcgaa tccgagataa tacctatgct tatctgattg tagatgattt gaacaattta
    55561 gtacgaggag gacgtttgac aaatggtgca gcgctgattg gcagcttatt aaaaatcaaa
    55621 ccgatcctga cattttctga agggaaaatt gttttattcg aaaaaatccg ttcaagtaaa
    55681 aaagcatttg ctcgagctga agaaattatt ggtagaagaa ataacgaaat acaaccgcca
    55741 gtcaaacttt acgtgataca tgcaaataat ttagaagtag cgattgcgga gaaagagaaa
    55801 ttgcagaaaa aatatccaaa tgcagaaatc gaaattggtc atttcggacc agtcattggt
    55861 acacatttag gtgaaaaagc catcggcatt gccatttcag cgcaataatt ccttttattc
    55921 aaaaaacata ccaaaaatat
//
LOCUS       DACDXO010000011        52130 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E23605_2017
            SAMN12639989-rid9881463.denovo.011, whole genome shotgun sequence.
ACCESSION   DACDXO010000011 DACDXO010000000
VERSION     DACDXO010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639989
            Sequence Read Archive: SRR10023389
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 52130)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 52130)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741914.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:42:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,972
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 33 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 17 of 73
            Pseudo Genes (multiple problems)  :: 10 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52130
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639989-rid9881463.denovo.011"
                     /isolate="E23605_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            320..1165
                     /locus_tag="IWM35_000804"
     CDS             320..1165
                     /locus_tag="IWM35_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP9918675.1"
                     /translation="MDEMKLIGIDLDGTLLNSDQSISQKNARTMKHLPEDCFPFICSG
                     REVEDINNILKKSGLRIPAVGLNGSVGFDGEKKIFESSFDKNDVKKIDSVLSKFPTKI
                     YTNLGSYESQNYKNKMKKIFHEVGSEFSIKELNYELEYEKSICSIPYNNIEEIIEKDA
                     ISIYKFFIFIPNRQLKANIFNHLKKMLNVSVTESAPVNIEIVPQNVSKGVVFDRLESI
                     YNLKKPLRIAIGDSLNDLSMFESADISFAMGNSHQEIMKKADYVTVSNDKDGVSQAFE
                     RILTI"
     gene            1446..2666
                     /locus_tag="IWM35_000805"
     CDS             1446..2666
                     /locus_tag="IWM35_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HAP9918676.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            2663..5383
                     /locus_tag="IWM35_000806"
     CDS             2663..5383
                     /locus_tag="IWM35_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP9918677.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            5367..6311
                     /locus_tag="IWM35_000807"
     CDS             5367..6311
                     /locus_tag="IWM35_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP9918678.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
     gene            complement(6380..7390)
                     /locus_tag="IWM35_000808"
     CDS             complement(6380..7390)
                     /locus_tag="IWM35_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP9918679.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
     gene            complement(7570..7914)
                     /locus_tag="IWM35_000809"
     CDS             complement(7570..7914)
                     /locus_tag="IWM35_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAP9918680.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            complement(7907..8341)
                     /locus_tag="IWM35_000810"
     CDS             complement(7907..8341)
                     /locus_tag="IWM35_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HAP9918681.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            8479..9216
                     /locus_tag="IWM35_000811"
     CDS             8479..9216
                     /locus_tag="IWM35_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9918682.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            9209..10420
                     /locus_tag="IWM35_000812"
     CDS             9209..10420
                     /locus_tag="IWM35_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9918683.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            10627..11406
                     /locus_tag="IWM35_000813"
     CDS             10627..11406
                     /locus_tag="IWM35_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HAP9918684.1"
                     /translation="MEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAAL
                     SREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLKT
                     MLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHGD
                     VNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDEM
                     LEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            11476..12138
                     /gene="trmB"
                     /locus_tag="IWM35_000814"
     CDS             11476..12138
                     /gene="trmB"
                     /locus_tag="IWM35_000814"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HAP9918685.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(12236..13990)
                     /gene="ade"
                     /locus_tag="IWM35_000815"
     CDS             complement(12236..13990)
                     /gene="ade"
                     /locus_tag="IWM35_000815"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HAP9918686.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNKQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            14122..15051
                     /gene="cpaF"
                     /locus_tag="IWM35_000816"
     CDS             14122..15051
                     /gene="cpaF"
                     /locus_tag="IWM35_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component"
                     /protein_id="HAP9918687.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGTVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLKLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            15008..16057
                     /locus_tag="IWM35_000817"
     CDS             15008..16057
                     /locus_tag="IWM35_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGB"
                     /protein_id="HAP9918688.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            16054..16332
                     /locus_tag="IWM35_000818"
     CDS             16054..16332
                     /locus_tag="IWM35_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HAP9918689.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            16307..16777
                     /locus_tag="IWM35_000819"
     CDS             16307..16777
                     /locus_tag="IWM35_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HAP9918690.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            16794..17096
                     /locus_tag="IWM35_000820"
     CDS             16794..17096
                     /locus_tag="IWM35_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9918691.1"
                     /translation="MESLTALMIASVCVYLVSFGQLFLLQAEKKSGEELLFIRMIYED
                     IKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAISIEKTSNSSIYAD"
     gene            17113..17484
                     /locus_tag="IWM35_000821"
     CDS             17113..17484
                     /locus_tag="IWM35_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComGF family competence protein"
                     /protein_id="HAP9918692.1"
                     /translation="MILSSLLLTFNLLIQQTIQVSEFLQRKDQKEWLIFLAQLEEELK
                     SSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQPMLTSITELQLLEKEHTI
                     SFYVHFENGEDGYAIWTKNDQ"
     gene            17456..17815
                     /locus_tag="IWM35_000822"
     CDS             17456..17815
                     /locus_tag="IWM35_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9918693.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            17987..18994
                     /locus_tag="IWM35_000823"
     CDS             17987..18994
                     /locus_tag="IWM35_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP9918694.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            19019..20203
                     /locus_tag="IWM35_000824"
     CDS             19019..20203
                     /locus_tag="IWM35_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HAP9918695.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(20640..22223)
                     /locus_tag="IWM35_000825"
     CDS             complement(20640..22223)
                     /locus_tag="IWM35_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAP9918696.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIALVDINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHCQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(22801..22944)
                     /locus_tag="IWM35_000826"
     CDS             complement(22801..22944)
                     /locus_tag="IWM35_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HAP9918697.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            complement(23166..23645)
                     /locus_tag="IWM35_000827"
     CDS             complement(23166..23645)
                     /locus_tag="IWM35_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAP9918698.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            complement(23718..24002)
                     /locus_tag="IWM35_000828"
     CDS             complement(23718..24002)
                     /locus_tag="IWM35_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9918699.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            complement(24080..24274)
                     /gene="ssrS"
                     /locus_tag="IWM35_000829"
     ncRNA           complement(24080..24274)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="IWM35_000829"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(24346..25632)
                     /locus_tag="IWM35_000830"
     CDS             complement(24346..25632)
                     /locus_tag="IWM35_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HAP9918700.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            25770..26441
                     /locus_tag="IWM35_000831"
     CDS             25770..26441
                     /locus_tag="IWM35_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HAP9918701.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            26407..26895
                     /locus_tag="IWM35_000832"
     CDS             26407..26895
                     /locus_tag="IWM35_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HAP9918702.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            26908..27855
                     /gene="prmA"
                     /locus_tag="IWM35_000833"
     CDS             26908..27855
                     /gene="prmA"
                     /locus_tag="IWM35_000833"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HAP9918703.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            27856..28620
                     /locus_tag="IWM35_000834"
     CDS             27856..28620
                     /locus_tag="IWM35_000834"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HAP9918704.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            28853..31066
                     /locus_tag="IWM35_000835"
     CDS             28853..31066
                     /locus_tag="IWM35_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HAP9918705.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            31077..31523
                     /locus_tag="IWM35_000836"
     CDS             31077..31523
                     /locus_tag="IWM35_000836"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HAP9918706.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            31694..32953
                     /locus_tag="IWM35_000837"
     CDS             31694..32953
                     /locus_tag="IWM35_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HAP9918707.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(33042..33989)
                     /locus_tag="IWM35_000838"
     CDS             complement(33042..33989)
                     /locus_tag="IWM35_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HAP9918708.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            34383..35675
                     /locus_tag="IWM35_000839"
     CDS             34383..35675
                     /locus_tag="IWM35_000839"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HAP9918709.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            35690..37459
                     /gene="aspS"
                     /locus_tag="IWM35_000840"
     CDS             35690..37459
                     /gene="aspS"
                     /locus_tag="IWM35_000840"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HAP9918710.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            37568..38443
                     /locus_tag="IWM35_000841"
     CDS             37568..38443
                     /locus_tag="IWM35_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP9918711.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            38709..40592
                     /locus_tag="IWM35_000842"
     CDS             38709..40592
                     /locus_tag="IWM35_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HAP9918712.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(40667..40951)
                     /locus_tag="IWM35_000843"
     CDS             complement(40667..40951)
                     /locus_tag="IWM35_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HAP9918713.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(40941..41675)
                     /locus_tag="IWM35_000844"
     CDS             complement(40941..41675)
                     /locus_tag="IWM35_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HAP9918714.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            41804..42427
                     /locus_tag="IWM35_000845"
     CDS             41804..42427
                     /locus_tag="IWM35_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HAP9918715.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            42619..43740
                     /locus_tag="IWM35_000846"
     CDS             42619..43740
                     /locus_tag="IWM35_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HAP9918716.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            43747..46875
                     /locus_tag="IWM35_000847"
     CDS             43747..46875
                     /locus_tag="IWM35_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HAP9918717.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANERIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            46894..50088
                     /locus_tag="IWM35_000848"
     CDS             46894..50088
                     /locus_tag="IWM35_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAP9918718.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(50234..50941)
                     /locus_tag="IWM35_000849"
     CDS             complement(50234..50941)
                     /locus_tag="IWM35_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAP9918719.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
     gene            51706..52104
                     /gene="spxA"
                     /locus_tag="IWM35_000850"
     CDS             51706..52104
                     /gene="spxA"
                     /locus_tag="IWM35_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HAP9918720.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
BASE COUNT        17107 a         9063 c        10786 g        15174 t
ORIGIN      
        1 gatgaactag aaatagttca tctttttttg tttaaatctt ttgctaagtt gaactaatag
       61 agtcttttat gaatatcgaa gttagttttt cacctaactt cgttttttat agttggttat
      121 tataaaatag taatatagct gatttggatt caattcatat ttatttataa atcaaaaata
      181 cagagaatag tatataaatg tcctttccta ttcttaaaat gatgtttgta cttacttaaa
      241 ttaggtataa ttagtatatg ttttgtgaga aatttagact tacaataagc agtgaattta
      301 taatcaacgt ataggagaca tggatgaaat gaaactaatt ggtatagatt tagacggtac
      361 tcttttgaat tctgaccaga gcattagtca aaaaaatgca agaacaatga aacatttacc
      421 ggaagactgt tttccattca tttgttctgg aagagaagta gaagacatta ataatattct
      481 caaaaaatct ggtttaagga taccagcagt agggttaaat ggttctgttg gatttgatgg
      541 agaaaaaaag atatttgagt cttccttcga taaaaatgat gtaaaaaaaa ttgattctgt
      601 cctatcgaaa tttccaacca aaatatatac aaatctcgga agttatgaat ctcagaatta
      661 taaaaataaa atgaaaaaaa tttttcacga ggtagggagt gaattttcta tcaaagagct
      721 aaactatgag cttgagtatg aaaaatctat ttgttcaatc ccttataata atattgagga
      781 aattatagaa aaggatgcta tcagcattta caaatttttt atttttattc ctaatcgtca
      841 attgaaagct aatatattta accatttgaa aaagatgcta aatgtgtctg ttactgaatc
      901 ggcacctgta aatattgaaa tcgtacctca aaatgtatct aaaggtgtgg tgtttgatcg
      961 tttggaatca atctacaatt taaagaaacc tttgagaatc gcgatcggag atagtttaaa
     1021 tgacttatct atgtttgaaa gtgcagatat tagttttgca atgggaaata gtcatcaaga
     1081 aatcatgaaa aaagctgatt atgtcactgt ttcaaatgat aaagacggag tatctcaagc
     1141 atttgaaaga attcttacca tataaactaa ttaaggtagt atagaacata gaattctaaa
     1201 tacggaaaaa tggagcataa attccagtac aaatagtact tcttcagtgt caaacgaagc
     1261 gaatataaca tagatattta ggattgtgac aaaaatcatg tcacaatctt tttttcgaat
     1321 aaacttattt tttgaagtcg gccatttatc gtaatttctt tgcataatac gaacaaattt
     1381 tcggttattg atgaaaaaaa acattgaaaa tggtatgata aaagaaaaaa aggaggaaat
     1441 ttttaatggt gaaatttatc catgcggcag atttacatat ggatcggtca tttgaaggac
     1501 tgacaactct tgataaaact gtacaggaaa aactattgaa gacgaatctg accgttttat
     1561 caaatatcat tgaacaggca ataataaata acgttgattt tgttttactt gcaggagata
     1621 attttcatca aaatcgtcct tcattaaaaa tacagaaaca tttttcagaa cagatgaaac
     1681 ggttagaaaa gaaccatata cctgtattta taatattcgg aaatcatgat ttctatcaaa
     1741 aagagcggta ttggttctcc tttcctaaaa atgtccattt atttacatcc gaaacggtag
     1801 agacaaaaaa aataacgata aaaagtggag aaactgtctc tctcagtgga tttagttatc
     1861 gtcaaccgtg gattcaaaaa gacaaagtga tggaatttcc ttctagagaa ctcacagatt
     1921 accatattgg gttgtaccac ggggaacctg gcgtctctca aaaaggaaac tatgctcctt
     1981 tccaaccctc aaagatgcaa gaaaagggat acgattattg ggctttaggg catatccatg
     2041 tgccaacggt cttaaatgaa aagcaaacaa ttgtttatcc aggtgcgcct caaggtcata
     2101 ctaaaaagga acaagcttct acatctattc tactagttga attaagtaaa ggaagttgcc
     2161 aaatacatcc gattaaagta gcagaggtct attggaagac aaaggaaata tcattgagga
     2221 aagcaagaac gacaaaagag gtcattgttc acattcgtca gcagctttca aaagtagagg
     2281 atggatttag tttaattgag ataaaattaa aagattatga ccatctcaat acagatgttt
     2341 tggaacggat acaatcagga gagctattag actatttgtt agaggaattc tcagatagga
     2401 taaatgattt ctttatttgg cgtatttctc tcatagagaa tactttttat acgaagaccc
     2461 cacttgctgc ttctgctaaa ttgatggaac agttatttca atactatcag acacctcaag
     2521 atttccaaca aattttaaat gaagtatata gtcatcaaga agcggctaga atattgagcg
     2581 aactgccgga atatcaagaa gaaacacttg aaaaagccaa gcagctgctt aatcaagatt
     2641 ttttatttga ggaggatcag gaatgaagat tttaaggatc gagattgctg cttttggaaa
     2701 atggcgtcaa aaaagttttg acttttattc aggcaatcag ctgatctatg ggggaaacga
     2761 agcaggaaaa tcaacgattt atcaatttat tcaagcaata ttatttggtt tcccttcaaa
     2821 aggccgtaaa aaaaaggatt atacaccaaa agacggctca gcctatggcg gcaaaatatg
     2881 gcttaaacat cctgtatatg gtgaatttgc tgtcgagcgc tacaagcagc aaaatcgcgg
     2941 aaaatcgaag gtgtggctgg gggatcaagt gggaagtgat gagttactag aaaaactgat
     3001 cgcacctttg acgaaagagt tattccagca ggtatttact ttccagcaag agcagttgat
     3061 ggaactagat catttgagag aaaaagagct acatgatgcg ctgatttctt taggcatcac
     3121 gggcagttct caagtattcc aaaagcggca agaatattat cagaaagcac aacaattatt
     3181 caagaaaaaa ggccaaaaat tactaatcaa tcaaaaatta aatgaatgga aagagctgca
     3241 agaaaaaatt catcaaaaac aagaacagga agcttatttt ggcaagttga tccaacaaga
     3301 acaggaatac gagtcgcaac agcagcagtt attggcacaa ttaaaagagg cgaacaaaca
     3361 attttcatta gccagacagc aagaacttaa tttttcttta tatgaagaat ggcaatctct
     3421 caaaaaaaac agcactacag gtcttcctga tgagcaactt cttttagatc tggagtcttt
     3481 ctccaaacgg tatcagcaaa caaatgagcg tttggaagaa ttagaagcaa cactggaaaa
     3541 gcatagcggg atggatcagc agtcagccag atattatttt tatttagagc aggagcagga
     3601 actaaaagat ctccagcaac ttcaatttac agcggaacaa ttaaacgaag aatggcaaag
     3661 attacaaatt gagactagag aaacaaattc agaattgaag attttggaac aaaggtggca
     3721 ttggcagaaa aatccgttac cggaagaatt tttatatgaa gaagaatgga agcatcttct
     3781 ggctgaagaa agaaggattg ctgatctaga aagccagatt tctgtgcgtc tccaaatagc
     3841 taaagaacag caagagatga ttgaagcaga agtgaatgac ttggaagaga cctatccagc
     3901 attattacaa aatcagtcag agatagcacc agtctcttca tctaaaccgt atatgctgat
     3961 ggctggcgga gcaataatca ttgcaggtat atttgtacca atgcccttga aagtgatcct
     4021 tttactaatt ggtgcaggca tgattggcta tttcttctac aaaaggcaac caaaaattaa
     4081 aactaaaaat gatttctctg agataaaagg aatgtggcaa gaaaaactag gacaacttga
     4141 tctagcctta gctcaattag ctgaagcaga agaaaaacaa aagagaactg gagaagaaaa
     4201 acagattctt cagcaagaag tatatcgttt tgcggatcat cggcacttag gaaagatgga
     4261 tacgctcaaa ctcatgaaag aatatggaca ggaagtcata gattatcaga aattatatgg
     4321 cgtatttgag cggaaacata aaagagaaca ggaaatacaa gaaaaactac gacaaatcga
     4381 ccaaaaattt gagtttgtta aagaatggtt accgatacaa gataagacaa tttcagaaaa
     4441 aatggtattg ctttcccgtt ttatacaaga gatggaacag atgaaatttg ccagaagcta
     4501 tcagcaaaat actttattga agcaagaaat caatcaattg aaaaagaaac aaaaagtatg
     4561 gatacaagaa tacagcgaac aattgactca agcaggctta tcttatcctt cggaagtacc
     4621 tgtcttttta caaaaagcca aacaagccga acagcagatg aaaagataca atgaattatc
     4681 ggaaatgctt actccactat ttcctgaaga gctgacaaaa gagaaattat ctgtaagact
     4741 gcgccaactg aaagaaaagc aatcccaact ccagcaagaa gaaaaagaag caagtgaaaa
     4801 tcggcaacga ctccaacttc aagtagaaga gttgcaaaga gacggcacat tggacgaact
     4861 ttatcaacaa gctagccggc aaaaagcaaa attacaggaa ttactggagg aatggggagc
     4921 acttgaaatc gccgggacat tcttaggtga tctttcaaca gagatgagtg aaagacaatt
     4981 accccagttg ttaagagcag caggtgatta tttaggtatt ttaacaaaag gaaattatca
     5041 gaagatatta ctgaaaaatg atacactgac tttaacagac ggatatacag aatttcccat
     5101 ctatgagtta tctacaggta cgaaagatca gctgattatg gcgatgagat ttgcttacct
     5161 ctcattagaa gctaaccgtt cgatatgccc gatcatcatt gatgatggct ggcttcatta
     5221 tgatcatcag cgaaagtatc aattagctga acttttagct aaatttggag aaaagtatca
     5281 aataatttgt ttttcttctg ataaagaaat ggtaagctat taccatgaga tgaaccaaat
     5341 cgtaaaacaa ttagaaggag caaataatga aaaaaattcg tgatttagta atcgatgagc
     5401 agtttgaaca atttgtcctg atcaaaagtg cagatgtaag aatcgctaaa aacggaaaaa
     5461 aatttattgc ttttactttc caagatactt caggtactat cgatggtaaa tactgggatg
     5521 cttcggagga ggaaatcaat aaatttacag ccggcacagt cgtttttcta aacggtaaaa
     5581 gagaggttta tcaaggaaac cctcaagtaa agattcttca tatgcgctta acaagggtag
     5641 atgagccagg agagcctagt ttatacatgg aaagagcccc tttgaagaaa gaagaaatgg
     5701 aagaagaaat caatcaattg ttgtttgaga tcaccaatgc gcattggaat cgtattgttc
     5761 gctatctact taataaatat caaaaacaat tttttgagtt tccggcagca aaaagaaacc
     5821 atcatgcatt tgccagtgga ttagcttatc atactttgac aatgcttcac ttagggaaat
     5881 caatcgccga tgaatatcct gagctgaatc gagcattgtt gtatgctggt atcattttgc
     5941 atgatttagg aaaggtgatt gaactttccg gtcctatgtc gacagaatat acgcttgcag
     6001 gaaatttgat cggtcatctg gtattgattg acgaagaaat aactaaagcc tgtcttgcgt
     6061 tgaaaatcag cgaagctgac gaagatgttc ttgtattacg tcatatggtt ttagcacacc
     6121 atggcttatt agaatatggc tcacctgtac gtccaagaat aatggaagca gaaatattac
     6181 atcaaatcga caatatcgat gcttcgatgc agatgatgtt gacttcgatt cgtcaaacag
     6241 aaccaggaaa atacaccgat cggattttcg gtatggacaa ccgaagtttc tatgtaccaa
     6301 aagatttata gcaaaaattt atttttttca ttcaaaacaa aaaaagcgag acataagtct
     6361 tcgctttttt tgttttcttt tattttgatg aatcagttgt ttcttcagtt gaagattctt
     6421 ttgttgaatc agttgatttt gtgtcagaac tctttgttga agctgtagtt tcacttgagt
     6481 ctttcgtact tgatgaagtt gttgtgaatg cactcaacac attttcaaag gcatcatctt
     6541 tgatttttac atttgcatct tttaattctt caccgattac tttagttgtg aatgcattgt
     6601 cacttagttt agtttcagta gcgatatctt ttagttgatc tttgtattta tccatgtcat
     6661 tgcctttatt ttggtttttc accattttga caacgtagta ttctgtcgca taagaagtag
     6721 gatttgttgt agtaataaca tcggagatct caccgtcttt caatttgaaa gctgcttctt
     6781 ttacttctgc aggaatagtt gttgtcgttg agtcgaattt aactttaccg ccatcttctt
     6841 ttgtctctgt atcagttgat ttgtctttgg ctaatttaga gaaatcatct ccatcatccg
     6901 ctgatttttt cacatctttt gcttcatctt cacttgataa tttgatgatt tgtgcttcta
     6961 cttctggatg gaaagatttc caagcagttt tcaaatcgtc atctgtaata tctacgtgag
     7021 ctttcaagcc agcttcaaaa gctaaattgt tacgaatata ttctttataa gtatctttcg
     7081 tatatccagc agcttctaat tgtgattcaa aagtatcacc taatgatttc gcttgtttgt
     7141 cgtattctgc atccacttgc ttatcggtta ctttatcgcc atatttgtta ttgaatactt
     7201 tataaatgat catccgttgt actaaggatt ggttcgatga ttcaagtttt gcttcatcat
     7261 agaaatcaga tacagtgatc gttccgcctt tcatcgttgc gatatcttta ttagtatcgc
     7321 ctgaacaagc tgctagagtt aaaacagcca acgcactagt agctgccaaa ataattgatt
     7381 tttttttcat taaaatttgc actcctgtaa ttagttatta tttttatata ttaactatac
     7441 catatcccat ttcctttaaa aacgttgtcg tggtagtttc aaacaaaatt caaaatttca
     7501 tcacattcct aggaaatacg caaacttttc tcacttatat agttaaatct gttgagaaaa
     7561 taatcattat tatatttgtt caggatactc tggcagatca gcttttagtt tttctacttg
     7621 ttcattgatt tgtgtgatac gtggttcggc ttggaattta aaatcttcga tatctttttt
     7681 gattgcttct tggaaagcag gaatcacaga ttcagctgtt tcttttgttt cgatcaatgc
     7741 gcttttaaaa tgattcaggc tgttatttaa atcatttgtc agagttgttg cttcttccaa
     7801 ttcttcgatc agtttctttc ttgtttcatt cccgctcctg ggcgccaata acagacctcc
     7861 gacagtgcca gcagcagctc caaataacag tcctttgaaa aagtttttaa tcatcgcagg
     7921 acacctgctt tacgattgtt tcttgaatag cttttagttc ttccgatcca taactatctt
     7981 gatgattgac aaagtgaata gaaaaatcat cctctttgct gtagcgaggg actaaatgga
     8041 tatgtgaatg aaaaacagat tgataggcta gttcacggtt gttattcaag atattaagac
     8101 ctttcatttc tggaaaagct ttttccaatg cttgagcaat cttaggcaca cgcgaaaaaa
     8161 cctctcctgc aagttcggga tcatattcaa aaatatctgc tacatgacgt ttaggcacaa
     8221 ctaatgtgtg tcctttcgtt gcttgagaga tgtctaaaaa agcatagacc ttgtcgtctt
     8281 catatatttt atagcttggg atttcttgat tgatgatttt gcaaaaaata cagttttcca
     8341 tataaaacat cctttctata aagaataaat acttcagctt aactttacca taagttaaag
     8401 ctattgtaag gaaaaaaaca aaataccgtc attttcttcg tggtataata aataaaaata
     8461 aatggcggag gaaaaagaat gagtttaatt atcgatcatg tcacaggggg gtacggacat
     8521 ctccctgtgt taaaagatat cagtttcgat gtacagcctg gagagatagt agggttgatt
     8581 ggactgaacg gagcagggaa aagtacgacc atcaagcaca ttatcggtct gctggaagct
     8641 tcaaaaggaa cgatagaaat caatcatcta tccttaaaga aaaatacgga agaatatcgc
     8701 aaacaaattg ggtatattcc agagactcct tctttatatg aagaattgac attgagagaa
     8761 catatcgaag tcactgcgat ggcctacgat atacctatgg atatagcaat gaaaagagca
     8821 gaaaagttgt tggagacttt tcggttatct aatcgtcttg actggttccc agctaatttt
     8881 tcaaagggaa tgaaacagaa agtgatgatc ttatgcgcct ttttgatcga gccttcttta
     8941 tatatcatcg acgaaccatt tttaggcttg gatccactag ctatcaatgc attgcttgaa
     9001 ttgatggtag aaatgaaaaa agaaggagcc ggtattttga tgtctacgca tattttagct
     9061 actgcagaaa aatattgtga tcgttttgtg gtcttacata acggagagat acgagcagac
     9121 ggttctcttg agatgttgag aaaagaattt cagctgccgg attcttcttt ggacgaaatc
     9181 tatattgctt taacaaagga gcaggcacat gagtagtttt tttcaaaaac gtttagcaag
     9241 acatcaaaag aaaatgatga aatatatgcg ttatgtattg aacgatcatt ttgttttagt
     9301 ttgtcttttc ttgattggcg gtgtgggatt atattattct aattggttga aaaccctgca
     9361 gccaccgttt gcaattggag ggctcataat ctctgtattt tggatgctct gtttattcgt
     9421 gggtaagatg gctacttttg cagaggcagc agatattgtt tttttacttc ctaaagaaaa
     9481 agaaatgcaa acctatttgg aacgagcttt tcgctattct tgcatttttc cgttgatctt
     9541 tttgatattg gcatgcggat tggccatgcc attagtggtg gttagtacgg agcaatcctt
     9601 ttcgatgttt ttcgtctatc tgatcatatt atggagtttg aaattcagtc atctgcaaat
     9661 caagagagca gaattgtttc gactagatca acaagttatt cgttattgga aattaggctg
     9721 gtttttttct tctctgatct ttttgtttct atcattttat tggctaatat gggcaggaat
     9781 agcaggtgca ttagtacagg caagtctata ttactggttt ttgtggaagc aacaagaaaa
     9841 aggattggat tgggagaaaa tggtgcatgt agaagaacat aggttgcatc agatctatca
     9901 atttatcaat ttattcacag atgttcctga aattacggca aagatcaaaa gaagacgcta
     9961 tttggatcct atattgactc atattaaaag agaatcaaaa aacacttatc tttacttgtt
    10021 tgcaagaaga ttggcaagag gttctgattt tagtggtctt tatttccgat tagtaattat
    10081 aggtgggtta ttgattgccg gagtagtcga cttttatttt agtctgatta tcggtttctt
    10141 atttttatat ttgatcggtt tccaactgtt acctttagcg aaccagttcc gctatatggt
    10201 tctcgttcag ctttatccaa tccataagaa tcagaagaag caggcgattc agtatctact
    10261 cacatgggta ttaattatcg cgtcggttat ttttggtttg attgctgcag tcgtattaga
    10321 tgggcagcag cgatgggttc cgctagcggc ttatttgatt gttactatgc tctttgttaa
    10381 actatatgta ccaactcgtt tgaagaagat gtcagactga aaacaaagct gtgtagggta
    10441 aaaatgagaa tgaaaaatag agcgttgaca gtaagaatgc tgttcgttct attttttttg
    10501 agaaaagctg gttttttgaa tggcaattaa atgaaaaaga acgtatatgt gcttgacgtg
    10561 tagtgttcag gcgactagaa taataagtac tgcaaaacaa agagtaagag gacgggagaa
    10621 tgcatgatgg agtttcagtt agaccaagag tggcggttac gtcccatcaa aggcgacaca
    10681 ggaaaaacat acataggcat gcgtgatgag gacaaagtct ttattaaacg aaacacgact
    10741 ccgatgttgg ctgctctttc aagggaagga attacaccaa aacttgtttg gacaaaacga
    10801 acggggaatg gcgatacttt gacagcacaa gagtggctgg aaggtcgctt gctgactcct
    10861 aatgagattg gtcaaagaaa cgacgtgatt gatgttttgt accatttaca tcattcaaat
    10921 tctcttaaaa ccatgctgac ccgtatcggt ggtcgtgtca tgacgccgga gaagatgctg
    10981 caaaaatatg aaaaagaatt accggaagaa ctccagaaaa atcaatttct gcaaattgtc
    11041 catcgttttc ttcgtgagca gatcccgtca atagataagg aaaacttatc agtagtacac
    11101 ggagatgtaa accatcgtaa ttggttagta tgtaaaaatt atttatatct agtcgattgg
    11161 gattcaatca tgtttgcaga ccctgcggtg gatattgcaa cgattttagg aaattatgtg
    11221 ccactgtcta gctggaatca gtggctgatc agttatggca tacgcccgac tgacgaaatg
    11281 cttgaaaaat ttcattggta tgcggccatg aatttgctcc aagaaatcac cagatattgc
    11341 aaacgtgaag atcatcgacg gatgaacgaa gaaattttac aattaaaacg aattttcagt
    11401 ggctgaggtt tggcccaaac tctgcggaga tttggccaga cctttttaat tagaggaaag
    11461 gaatggagat aaataatgcg tgtccgaaat cgtccgggag cagcggaaat gttagcggct
    11521 cacccgaatt ttgtaattag tgatccaaca ttgtggaagg gaaaatggaa tgaattgttt
    11581 gaaaacgatc atccaatcca cattgagatt ggtatgggga aaggccagtt catcactgga
    11641 atggcaaaag cccatcctga aatcaattat attggagtag aaatgcaggt cagtgttgtt
    11701 tcgattgcac tagacaagtt gatcgaacaa ccgcttccta acctgaaact tctacatgtg
    11761 gatggatcag cgttgacaga gtatttcgct gacagcgaag tagatcaaat ctatctgaat
    11821 ttttccgatc catggccaaa aaaacgtcat gaaaaacggc gattgacata taagactttt
    11881 cttgctgtag atgaacagat tcttcgtcca aatggagaaa tccatttcaa aacagataat
    11941 caaggattgt ttgagtattc tctggcaagt ttttctcaat atggcatgat cctcaaacag
    12001 gtatggttag atttacatca aagtcaattt gaaggcaata ttatgacgga gtatgaagaa
    12061 aaattttctt caaaaggtca acgtatttat cgagtagaag ctcaatttca agataagacc
    12121 aaaaagaaaa gtctgtgaaa ctaactttct gctttcataa aaacattaat atccataaat
    12181 aaatctcaga aaatgaacat tacagatgta atgtattttc tgggatttat ttgatttaaa
    12241 atgtatttat ttcatcggtt tttccacaag gtggaatcaa ttggaagcga gaaaatgaaa
    12301 ataatccttt gtcggtgatc ttcaattcgg gtatgacaga taatgttaag aaagaaagtg
    12361 tcaaaaaagg atcaaaggtt ttagctgctc ctagttgatg tgctttagtt attaattcat
    12421 tcaactgttc atagacgatc gaaaaaggtt cctgtgtcat gattccgcct ataggaagag
    12481 aaacacaagc tatttcctgc tgtttgttga caacggtcat cccgccacct ttttgaatca
    12541 aaagattagc agcatacagc atatcctcgt cgtttgtccc aacgacaact agattatgcg
    12601 agtcatgggc aatcgttgtc gctatcgctc cttgtttcag ctgaaatcct ttcactatgc
    12661 ctaatccaat gtttcccgtt tgatggtgac gctcgatcac cgcaatcttt aattggtctt
    12721 tttcgattga aggaatgaat cgttcgcctt gtcggtccac tttttctacc aaatctttag
    12781 tcactagact attcgggata atttcgatca catgggcttg tgaagaagaa agaagcagct
    12841 ctaacgaatg tggatttaat ggttgagcgt ttagttttgg cagacaacac gtatatgaat
    12901 ttgaatgatc agcaaataaa tggtcttgga gttgcccaga ttgaacaact aatgttccct
    12961 tatagaaaac ttcatgaatt ggcagcttgg taagatcgtc tgttaagaag aaatcagctt
    13021 gatacccagg ggcaatcgca cctagtgttt tcaaatcaaa acattctgct gcattcaatg
    13081 ttgccatctg aatggcttgt aatggatcga ttccaagtgc aatagcttga cggacgatat
    13141 gatcgattga tccttctttt actaaatcat tgatcagttt atcatcggtg acaaagaagc
    13201 atcttcttga attttcaggt gtgatcgccg gtagtagcga tgccagatct tttgcaacgg
    13261 tcccttctcg aaccattaaa tacataccaa gatccaaacg ttctttagct tcttttgcag
    13321 ttgtagcttc atgatccgtc cgaatacctg ctgcaggata aacatttaac tcttccatgc
    13381 cgataccagc tgcatgcccg tcaatttttt tcttatgctg gtgcatcaat tggatctttt
    13441 ctaggatgtc tgtttcatta tttgctactg cttgatagtt catgacttca gctaagccta
    13501 aaacttcggg tttttgtaaa aaaggggcta atttttttgc atttaaattt gcaccattgg
    13561 tctcaaaagg ggttacaggt acacaagaag gtagcataaa aaagatattc atgagtgttt
    13621 gtcttgcatc ttctatcata aactcaagac ctgtactccc agcaatgttt cctatttcat
    13681 ggggatctgt cactacagta gtaactccat gaagcaagga tacttttgaa aattcgcttg
    13741 gtgataaaag tgagctttcg atatgcacat ggccatcgat gaatcctggc actagatatt
    13801 gcccattagc attatgtacg ctttttcctt cgtattcacc gattcctacg atcattcctt
    13861 cgcagattgc tacatctttt tccatgattt ctttcgtaaa gacgttgacg atcttagcat
    13921 gcttgatcac taggtcagca ggcttttttt tacttgctac ttcgatatat ttccttaatg
    13981 ttgtttccaa tggagtgcct cccaagataa atttttataa gtataacata aattcggctt
    14041 ttgccaatat ttttttgtaa attcatttta tttgaaacaa aaagatcaaa aagacgtatg
    14101 ttttttgtgg aggggagaat aatggaaata aaacgattat cggatcaaat gataagtgaa
    14161 agttttgcca aacatgccca agacgtatat atattgccag cttcagaagt attttacatc
    14221 tattttcgct atggaaaaaa acgaacattc tggaagaaag tctcttcggc agatggacag
    14281 caactgatct caagatttaa gtatttggga cagatggata tcggagaaaa aagaaaagct
    14341 cagttaggtg catttactta ttctttagaa aaacaggaag ttcgtctgcg gttgtcttct
    14401 gtaggagatt accttcaacg agagagttta gtgattcggc tattatacgg tacttttgaa
    14461 acaccccatc gttgctttag ttctcgagat tttgcaacta ttttacagaa aaccgtcaga
    14521 cgtggattat accttttcag cgggacagtc ggatcaggca agacttctct aatgtatcgg
    14581 ttggctttgg aagaaaaatt gcaagtgatt actatcgaag atcctgtaga gatagaagaa
    14641 ccgttttttc tgcagctaca gatcaatccg aaaatagcac agacttatga tacacttttg
    14701 aaactcgcat tacggcatcg tccagacttg ctaatcattg gcgagataag agatgaatca
    14761 actgcacatg ctgcggtaag agcagcacta acagggcacc gggtttttgc aaccgtacat
    14821 gcacgcaatc ttgaaggtac attggtcaga atccatgaac tgacaaagaa aaaagaagag
    14881 ctatctgaat gtttagctgg tgtcatttat caagaacttt tggaagatgc caatcaccat
    14941 ccttctgttt tatggagtta tcaatttatg gaggaagaat cagtaaaaaa atcatgggag
    15001 gaaagttatg aagaagctca ggaaaatatt tggcagtcag aactattcta aaggattaac
    15061 aagaagccag caaaaccagt ttgcacaaac catgggagaa ctgcttttat ctggttttag
    15121 tctgcaagaa tccctttatt tattactcac gatccgttcg tttccacctg ttattttggc
    15181 aaatatccaa catcagctaa aggaagggca agcgtttagt gaggtacttt cgcaaatagg
    15241 atatggagca gagcaactgg tacagatcga gttagctgaa cgtcatggga atctgccgac
    15301 aactttgttg gaaattgcca aacagtttcg gcttgttgaa ggatttcgca atgatttgaa
    15361 aaagctgctt gcatatccga tgctattgct cacctttttg ctaggtatct tgtttagtat
    15421 gcggttgttc gttttgcctc agcttattgg ttcaggaatg gtcatgcaag agcattgggg
    15481 tattcagctg atacaagtct ttcattggta tgttctacta ttcatcctag cggttactct
    15541 attggcttta ctagtaaggt atcgtctgat gaagttgtct cctgttgatc agagtgaatg
    15601 gataagcaga caattattga ttggaccgat atattccttg tatcaatcgg cttatttgtc
    15661 actagagatt ggaaagcttt ttcatgaagg tttcgaatta aggcaaatca tagtctgctt
    15721 aaaggaaacg aaacaagaaa gtctgattca gcatcttgcc tctaagttga ttcaaggatt
    15781 ggaggcaggt atgtcattgg ctgagcagtt ccagaattat cgttttctat cagaagaatt
    15841 cagtaaaatt gttttacagg gagaggcaaa gggaagttta ggtaaagaac tgatttttta
    15901 cagcgaatgg acaagaaaac agcttttcaa acgtcttcat cactggatgc attttattca
    15961 gccaatcatt tttttgggcg tagcggcttt gattttatta gtttatgccg ctgttttatt
    16021 acctgtttac ggaagcatag aggaggtttt gccatgaaaa agagaaaaat atcttattat
    16081 agcggtttta cattgatcga aatgctaatc gtcttactga tcatcagtgt attagtgttg
    16141 ttgttcgtcc ctaatttgtc gcgttatagg aatcatgtag accaagaaag tcgtgaagcg
    16201 atcatccagt tggtagacac acaaaaagag ctctatgcgc ttcaaaataa tggaagagtt
    16261 cctactgtgg aggaattgtt gaatgaaggc tatatcaaac gagaacatgc agaaatctat
    16321 cagcggcctt gaaggtaagg ttttagcatc aggatatacg ttgtttgaat cactcttact
    16381 gattttatta cttacaacga tcctttcttt tccagttctc gcattttctg catggaagaa
    16441 tgaattggca gtctatcaat ttttcggcca atttgaaaaa cgaatctatt ctacgcaaaa
    16501 aatcgctatt gtgaatcaga tccaaacagg cttttattgg aatagtgaag agaatcaaat
    16561 catttttcat gttccgaatc cagataaaat aaattgggaa gtattagata ttcctgatga
    16621 gattaccttg aaacgccatg cttctattac ttttgctgct agaaccggga atgaaagttc
    16681 actaaaagct tatcaatttt attgggaaga aaaagagcgg acgattacgt atcaattcca
    16741 gatagggagt ggtcgctata caaaaagaat tgaataaaac aggatttgtt ttgttggagt
    16801 cccttacagc gcttatgatc gcttctgtct gtgtctactt agtgagcttt ggccagctgt
    16861 ttttgcttca ggcagaaaag aaaagtgggg aggaactgct atttattcga atgatttatg
    16921 aagatatcaa gacgcagcgg atgtatcaca aaagaccaga gcgaagggaa gaaatgatcc
    16981 catacgatat ccaaattgtc gataataatg gagtaatcga aaaagtgttt gtcaatatgg
    17041 agaaagaagc gatcagtatt gaaaaaacaa gtaattccag catttacgct gattgaatgt
    17101 ctaattggac tgatgatatt gagcagtcta ttgctcacat tcaatctttt gattcagcaa
    17161 accatccaag taagtgaatt tttgcagcgt aaagatcaaa aagagtggct aatattttta
    17221 gcacaattag aagaagaact caagagcagt cacaatgtct ctgtgaaaaa tcagcaactg
    17281 cactacacga tcgaggataa acagtatatc atcgaaagat atcaggcaat gatacgtaaa
    17341 agaagaactt ccggcggaca tcaacctatg ttaacaagta tcactgagct tcaattattg
    17401 gaaaaagaac acacaatttc tttttatgta cactttgaaa atggagaaga tggatatgcc
    17461 atatggacaa aaaacgatca gtagaaaatg ttataagggc tctattttat tttctgtctt
    17521 gcttttattt cttttatgta cgtttttatt gatggcagca ttagaaagct accgtttgtc
    17581 aatggatctt aatatacgaa tcaaaaatta ttatatagca aaaataatga agcagatggc
    17641 tttatcggaa cagtctacgc ttgccgaaag ttcggaagga gttttttact acacaactgg
    17701 atctgtaaca tatcagtggg tacaacagtc cctattctta gaggtggaag tctcaccgtt
    17761 tatctttcgg ttcacagaag aagtaaaaaa tgatacagat actagtacag aatgatggaa
    17821 tgttcgtgaa aacgccctct tttctgttat aacactgttt ttaataaaaa aatgtttgcc
    17881 gtttcacaaa taaaaaacag tgaaaacgat tgaaattttg tcaattgttc gatagaatta
    17941 aacaggtgaa aaaattgtta taatatatct agaaatgagg tgcaccatgt taccagaaaa
    18001 aatggaacaa gggtttaatc aaaacctgga agctattcag cttttacaga atgcattggg
    18061 aacttccttt ttagaagcct atgtcgaaaa tgcagaaaat ttaatagatg attatcaagt
    18121 acgtgtggtg gatggagtgc cgacaaaaga gacaacgcaa cggatcacag ctctttatga
    18181 agagttgaag aaactttctt tcgagccaga agaatggcgc agactgtctc aattactttt
    18241 attgaagggc agccagacag aacatttgca ggcgaaccat caactcactc ctgatagtat
    18301 cggtttttta tttgtttttc ttattgaaca actatacaca aataaaaaag caccggttaa
    18361 gattctggat attgcggcag gaatgggcaa cttgttatta acagttcttt taaacctatc
    18421 gaatgcgggg taccaaacag aaggaattgg tgtggatatt gatgatacat tgttggctgt
    18481 cgcagcttct acaagtgatt tgacacaagc aaatgttcaa tactttcatc aagacgggtt
    18541 gcaggaatta ttaatagatc ctgtcgattt tgcaatcagc gatttgccaa tcggttatta
    18601 tccgaatgac gaaaaagcaa aagagttttt aacaagtacc gaagaaggcc atagttatgc
    18661 acatcattta ttgctagagc aatcaatgaa atatgtgaag ccggatggtt ttggtttgtt
    18721 tttgatgccg agtggatttt tagaaacgga tcaaagtgaa gaaatcaaaa aatggtttaa
    18781 agaagaaggt tatcttcaag gaatgattca attgccagat gaattgttcc gtaataaaca
    18841 atcgcaaaaa agtattttga tcttacagaa aaaaggacca caagcacaac aagtaaaaga
    18901 agtcttgctt gtgaaacttg cttcattaaa agaaccagag aaggtgacgg aatttttcaa
    18961 cgagtttaaa aactggaaat catctgctct gtaatcattc aacgagaagg agagtcatat
    19021 gtctaaaaca attgcaatca atgctgggag ttcaagtttg aaatggcaat tatatcaaat
    19081 gccagaagaa accgtagttg cgaaagggat cgtcgaacgt atcggtttac aagattctat
    19141 ttttaccatc aagtacggcg ataatcaaaa attcgaacaa atcatggata tcgaaaacca
    19201 cgaagtagca gtaaaaatgc tgttagatca gttgattgaa ctgcaaattt taagctctta
    19261 tgatgaaatc accggagtag ggcaccgtgt tgttcacgga ggagaaactt atggggattc
    19321 agtagtgatc gatgagaaag taatggaccg cattgctgaa ttagcagaat ttgctccttt
    19381 gcataatcct gcaaacttaa tggggatcaa agcgtttaaa aagattttac ctgacatttt
    19441 gagtgtggct gtattcgata catctttcca ctcaacaatg ccaaagcaaa attatttata
    19501 tagcttaccg gtgtcttatt atgaagattt caaagtaaga aaatatggct tccatggcac
    19561 aagtcatcgt tatgtgtcgg aacgtgcggc aaaattattg aacaaaccga tcgaagaact
    19621 aaaaatcatc acttgccact taggaaatgg tgtatcgatc acagcagtcg atggaggtaa
    19681 atccatggat acttctatgg gattcacacc attagctggg gtgacaatgg gtacacgttc
    19741 aggtgatatc gatccagctg ttctcccata tttaatggaa aaattagaga ttgatattga
    19801 tgaaatgatc aatgttttga acaagaaatc tggtctgcta ggcctaacag gattgtctag
    19861 tgatatgcgt gatcttgaaa agaactatga aaaagaacat attcgtttag cttatgacat
    19921 tttcgtggac cgcatccgaa aatacatcgg cggttatgtg acaacgatga atggtgttga
    19981 tgcaattgtc tttactgctg gtatcggcga gaatgacgga catgtacgta gtgaaatcat
    20041 caaaggtatg tcttggtttg gttgtgaaat cgatccagcg ttgaatgaat tgcgtggtga
    20101 agaagtagat atctctacaa aagaatcaaa agtaagagta ttagtgatcc caacagatga
    20161 ggaactaatg attgcccgtg atgtggagcg tctgcgcggc taagccaaag cattcgttag
    20221 gataaaagat gaaacgcatg caagagggaa ttcttgcatg tgtttttttt gcggagaaga
    20281 ccgcaaaatg gtaatttaac ttcaatttcc cttctttttt acaggatctt cttagggaat
    20341 tttgcttgga aaagtggtca aaagggaaaa gactggcatt ttatttattg attttcagga
    20401 aaatcccaat attttatttt cattcatctt tataagggac attacatatg taaaaagaaa
    20461 aaacattttt agatcagcct cgtgggctgt cgactttatt cttcgcttta tgtttagcta
    20521 gaaaaccgat ccgaaacatt cattgatcga ataataaaaa cttgattttc cacctgtact
    20581 ttgcctgaat cgtgaaagca caggtatgaa aatcaagttt tttgttgcga aaaaacgtcc
    20641 tagtcaatca gttggccgag cggctggcgg ctgatctgct cctgatcaat aacgagtgct
    20701 tcttggaagt gttgctgcaa aaagtagcgg tcatggctga ctgctaggag cgttccttga
    20761 tagtcttgca gcatttcttc aatttcttct cgagcatagc tgtcgaggtg attggttggt
    20821 tcatccaaaa tcaggacgtt gattttttct aaaaagagtt tcagcaacgc caaacggatt
    20881 ttttcaccac cggataaatc tttgatccgt ttacccacat cgctttgata aaagccactt
    20941 ttggctaatt gccgtcgagc gacttcttcg tttccagtca atgtgatggc gtaagccagc
    21001 aaacggctat cgggatcggc aaactgcagc tgctgcggca aataaccgat ttgcaagcta
    21061 ctacctaatt tgatggtgcc accgtaaggc ttttcctcac cgagtaataa tttgagtaag
    21121 gttgattttc ctgcaccatt gtcgccgatg atggctaaat gatctccacg atagactgca
    21181 aaactgctgt ctttgaatag tacttgatca gcgtactgct ggtgcaagtt ttgaatgatg
    21241 aaaacttctt tgccagattt tccaccatta gacaagcttt gcagccgatt tttcggggga
    21301 ggtggcggtt tgacgagtgt cagtttcgcc aggcggcgtt ccagttcttt ggcttttttg
    21361 aagaaggact cattgtctcc ttcatgtgcc cactgacgaa aacgacgaat catgactttc
    21421 aaccgctgga cttctttttg ctgcagttcg taattttttg tcagctcagc caagcgagct
    21481 tctttcaaga ctcgaaaacg gctgtagttt ccgggatact caatcaatgc accttcttcc
    21541 agttcccatg tttttgtggt gacttgatcg agaaattgcc gatcatgaga aacgaccaga
    21601 accgattttt ttgtattctg taaatagttt tccaaccagc gaatactgtt aagatctaaa
    21661 tgattggtcg gttcatcgag taaaagtaaa tcggcatcgg ctgctaaaat tttggctaaa
    21721 gcgactttaa ctcgttcgcc tccgctaagt tctgttaacc gtgcttcctg acagtgtgga
    21781 atccctaagc cttgcaagat cgctgcaatc cggtcttcta atacgtaacc gcctagttct
    21841 tcaaagcgat gctgcagctc accgtaacgg gccagacact tctctaagtt tgtatctggt
    21901 tctgtcatca atgcttcgta gcgactcatt tgctgtttga ggtcctgtat ctcagcggca
    21961 gaatggagca agtagtggtt gacggtcgct tcggaaactg taagttcttg ttcgaccgtc
    22021 ccgatcttta agcctttttt gcgactgata acaccgctgt cgacaccttc tctgccggtc
    22081 aagatttgta aaaaggttga tttgccagtt ccgttgatat cgactaaggc aattttttca
    22141 ccagcttcaa tttgacagga taaatcctca aataatggat tcccgccgat tgttttttta
    22201 atagaaattg cttgtatgat catgatctct ctccttgaga ccactcaaaa aggccatggt
    22261 tatccctggc ctgtgttatt taaactcaga ttatcatgag aaaaatacct tgagatggcc
    22321 attgatttta cgtgaattca actgttttgg acaaacttgt cccgtcaaca gcaactgtaa
    22381 aaccaattgc atggctaaat gcgatatggt atcgttggta attttcatta cttgttattt
    22441 tagtcatgcg catcatagta ttttccctcc ttcgtgtagt tgtcttttcc agtgtacctg
    22501 aaagtgtcag aaagttcaac tagcgctgac atttacttaa tatttatcga tgtgtaattc
    22561 tgtcagagag ctttttttat ctgactgagg gtaatgtctc gtggaccaca tcattgtatt
    22621 ctgagctaaa ccagcgttca ggcgaaatca tatggtgttt gataaaaaac tttgtctaca
    22681 tgacttcctt ttgccatttg cttaataccg ttgcattaaa cagctcttct ctttggaaat
    22741 ctcaccaaca tcgcgttcag gagtatcgtt gtggttgcaa tggcaagcgg tagcctttca
    22801 tcaattcttg aatgatttac cgaatgtagc acttgccttc tcgcggtacc cgaacccgaa
    22861 ataaaatttt agtttggcga taaaatcggt atccgccatc gatacttcat attttttcgt
    22921 atcagattca tcggaaactt ccacattgac gaaccaataa gctttggcat gcttaggtcg
    22981 ttttaaacca caccagggcg actaatcgcc ctggtgttca ctttttttat tttacttgaa
    23041 ctcattgact gttttccctt tattttaaac attataaaga ccgatgagtg cttgccgcgt
    23101 cttttgtttt tatcacggtt ttttgtgcaa aaaaacctgc cgatagttgg caggtcgtct
    23161 acgtgttaag ctttggtgtg gtctttagct ggcaattggt tttctaaatc agttcggaca
    23221 acaagcacat cgcaagctgc gtttctaata acatattcag aaacagaacc aataaataaa
    23281 cgttctacag cattcaatcc tgtggctcct aacataatca aatctacttg attatcttct
    23341 gggatttctc tggcaataat ttgttttggt gagccatatt ctacgacaga ggttacatta
    23401 ttcaagccag ctttcttcgc attgctttca taatctgcca atgtttgctt tgccatttca
    23461 gtagcttgtt ctgcaagcat tccatcaaag gatgaaacgg tttggaaaga acgtgtgtca
    23521 atgacatgtg ctaatagaag ttctccgtta tttcggatag ctacattgac cgccttttta
    23581 aaagctaatt cagcttcatc agaaccatct acggcaacca tgattttttt gtattgttgt
    23641 aacataagca ttcactcctc aagtcaatcg tattgtactc ttttattgta gaacacttta
    23701 gggaaaaaga gaattattta ctgctttggt tcagcaattt tttttgcaaa tcggatactg
    23761 aaggtggcag aaacaataat tacgatcagt aaaaagacca aggcaataaa gcgttgattg
    23821 aaaaaagctg ttaaaatacc cattacgcct aaaacagcat atgcagcacc gtaaaattgt
    23881 aggaatcctt gatttttatc atttttgttg atcaatacaa aaaagatatc ctgtttatta
    23941 aataaataat agctaacgaa taataatact aaagtggcga taatcattaa aatcctaatc
    24001 atgaacaaat cctccaaaat aagcaacaaa tagaaaaagc aatttgccaa gagcaaattg
    24061 ctttattctt taaaagatgg taatctccgt taatgccgtt gtttgtcctg tagtattgat
    24121 cccgcgaatt caagcaggtg ggtcccatgg tcgtagcttc gaactaccaa atgataaggc
    24181 atgttaccag cagtgacaca agacgggtcc caagatatca ataaaaatgt tcggtcaaca
    24241 cacaaagagg acaattcgaa catcacagat ttcccacctc tatagtagca tatgaattta
    24301 aaaagagcaa tgagagagct cgcagaaaca gaaccgtttt cttgtctagt tttttggagg
    24361 atgctttttc cactctttta tacggtaata ctgttgaccc agagcctgtt catatttccc
    24421 tgtatctttt ggctgataat actgagcatt cttcagttta tctggtaaat attgctgatt
    24481 gacccaagcc tgatcaaaat gatgaggata ctgataaccc acgccacggt tgagtgattt
    24541 cgcacctttg taatggctat ctctcaaatg atcaggaaca tctccagctt tgccctctcg
    24601 aatatcagca agtgctgcat ctaatgccat gtaggcagaa ttggattttg gcgacaagca
    24661 aagatcgacg acgacgtctg ctagtgggat acgtgcctca ggcaatccca atttttcagc
    24721 agctaataca gcgttcactg tacgagccgc agcagcagga ttacctaaac ctatatcttc
    24781 ataaccgata accatcagac gccggcaaat acttgccaga tcacctgcct caactaaacg
    24841 ggcgagataa tgaagtgcag catccacatc acttccacga atcgattttt gaaaagccga
    24901 aataacgtca taatgagcat ctccattttt gtcatgggta agagcttttc tttggacaca
    24961 ttcttcaatg atcgacagtg taagatgaat ccttccttct ttatctgaag gagtcgatag
    25021 tgtagccagt tccagcccat tcaatgcact ccgcaaatcg ccgttcgttg cacgcgaaag
    25081 gtgaagaaga gcatcttcgt ctaactggat tgcttgttgt ccaaggccgc gttctttatc
    25141 ccttaaagca tgttcgactg ccaactggat gtcttgttct gttaatggtt tgacttcaaa
    25201 gatctgtgta cggctgcgga tcgctggatt gatggtaata taaggattct cagtagtcgc
    25261 tccgatcaag atgattcggc cactttctaa atgaggaagc agaaaatcct gttttgtttt
    25321 atctaatcgg tgtacttcat ccaataagag gatcactgta ccactcattt ttgcttcttc
    25381 cgctacaact tgtagatcct tttttgaatc tgtagcggcg tttagcatgc gaaatgcata
    25441 attagtagag ccggcgatcg cacttgcgat actcgttttt ccagtaccag gaggtccata
    25501 tagaatcatg gaagagagca tgcgagcatc gaccatgcgg cgtatgattt ttccttcccc
    25561 gactagatgc tgctgaccaa cgacttcatc gatcgttcga ggtctcatgc gataagccaa
    25621 tggctgttgc ataggatttt cctccttctt gtttttacgt cccttaatta tatcatattt
    25681 agcaagatgc ctagaaagaa cagccttctg ttgttttgtt ttcatagctt atccaactaa
    25741 tattgtgtat gataaagtag gtgagaaaaa tgacggataa acaaaagata gatgaatttt
    25801 tttggaataa aacgacgcca gaagtggcac gggatttatt ggggatgtat ttggagcatg
    25861 acacaccaga aggaagatta gctggatata ttgtagacgc ggaagcatac ctaggtccag
    25921 aagacgaggc cgcccatagc tatggtttaa gaagaacacc gcgtgtgcgg gcaatgtatg
    25981 aaaaaccagg tacgatctac ctttacacga tgcatactca tcggattttg aacatcatta
    26041 ctcagccaga agggattcct caaggagtaa tgatacgtgc aatcgaaccc gccgcaatga
    26101 ttgatcaaat gagtaaaaat cgtggcggaa aaactggtcc agatatcagc aacggtccgg
    26161 gaaaattagt ggaagcatta gcaattcctc aagaacttta tggtcagtct atagcagata
    26221 gttcgttacg cttagttttt gagaaaaaaa agacaccaaa aaagatcaat gctttgccta
    26281 gaatcggtat cccaaacaaa ggtgtatgga cagaaaagcc gttgcgtttt gttgtgtcgg
    26341 gaaatcctta tatctcttta caaagaaaaa accaaatcga gaaagattgg ggttggagga
    26401 aagaaaatga aaaagaagga tctattaaca tatttagatg aaataattga aaaacaagtg
    26461 acagactatg atgtagcttt agattgggat acaaaaaatc atacgatcga gatcgttgtt
    26521 cgcttgtttg cagaaaataa agcacaattt gagattgatg atgcagaagg gatcgtttcg
    26581 caagaaccaa ttatcgaatt tgaagacggc gtacttcttt ttaatcctca gaaatctgtt
    26641 tttgatgaac gcaattatct agcagtaatc ccttatgaag gaaaaaaagg gttggcaaaa
    26701 tcagtagctg attctttagt ggaatatcta aatgaagtat tagctcaagg gcaaagcgat
    26761 ttgcttgatt ttttggatga agacgatgac gaagctgtat tcgagcttca ctgggataat
    26821 caacaactag cagaattagt agaagtaaaa cagcaaaatg aagcagatac ttatttaccg
    26881 tacccaagtt attaaggagt gacagatatg aaatggaatg aagtaaaagt agaaactgaa
    26941 agtgaagcag tcgaagcagt tgctaatatt ttgatggagg caggggcgag tggtgtcgcg
    27001 attgaagatt ctttagatgt tgaacatttt cagtcggatc aatatggtga gattttagat
    27061 aaaaatgatt ttacatcgct gaaagaaggc gcactagtca tggcttattt tccggaaacg
    27121 actttcttac cggaaatctt gccttttatc aaagatcgta tcacacaatt acctgagttt
    27181 ggtctagcta ttggaaaaaa taaagtaaca gttagtgaag tagaggaaag caactgggca
    27241 acagcctgga agaaatacta tcatccggtg cgtattacgc gaatgctgac ggttgcgcca
    27301 agctgggaaa actaccagac agaagatcct ctagaaaaaa ttatcacact agatcctggt
    27361 atggcatttg gcacaggcac tcatcccacg acttccttga cattgacagc actagaaaca
    27421 gtcttgcgtg gtggtgaaac attgctagat gtgggaactg ggtcgggcgt gctaagtatt
    27481 gcagcaaaat atcttggtgc aaaagatgtc tatgcctttg atttagacga agtagccgtt
    27541 cgttcggcga aagaaaatat ggatatgaat gaaatagcca aagatgtcca tgtatcagct
    27601 aacgatcttt tgacaggaat cgagattgaa agtgatgtaa tcgttgcgaa cattttagca
    27661 gatatcatct tattgatgat cccagatgct tggcgtttac tgaagcaaac aggcacgctg
    27721 atcgtttcag gaatcattga agagaagaag caaatggttc tggatgccat gttagaacaa
    27781 gggtttatcg tggatcaaat tttccagcaa aaagactggt atgcaattat tttgaaaaaa
    27841 ccagaggtgg aatagatgca gcggtatttt ttaaaagaat catatgaatc aaaagactat
    27901 tttgttttag aaggggaaaa ctatcaccac attgtacggg ttatgaggat ggaacccgag
    27961 gatgaagttt ttttggcatt caatgatcaa atagccattg ttgcagaaat cacggagatt
    28021 actgaagagg ctgtttatct gaaagaaatt aaaaaagaag aaaatcaaaa ggaactgcca
    28081 agttcgatta ccatagcgag tggctatcct aaaggggaaa agctggattg gatcgttcag
    28141 aaatccactg aattaggagc gcaagctttt cttggtttcc cggcatcagc ctctgtcgtt
    28201 aaatgggatg caaaaaaacg tatgaaacga caacaacggc ttgaaaagat tgctcaagag
    28261 gcagcggaac aatcccatcg gcagatcgtg ccatcagtag agttattgga aacgaaacaa
    28321 gatttatttg agcggctaga tgattatgac gtggtcttgg ttgcttatga ggaatcagca
    28381 aagcaaggcg aaaaatcaaa tgcagccaaa attcttagtg aaacaaaacc agatagtcga
    28441 atcttagctg tctttggacc agaaggtgga ttatcccgcg atgaagtcga ttattttgta
    28501 gaacacaaag ggatactttg tggattagga ccaagaatcc ttcgtacaga aacagcacct
    28561 ctttatttat taagtgctgc tagctatcaa tgggaattaa tgaatccttc tttcagctaa
    28621 cgttcttcat taataaaaga gtatcgacag aaagaattga aaaaatagtc aacaatttgt
    28681 ataatagaaa caattattta tacaatggat acgaaaaatc gtttgaaaaa gcttttaaaa
    28741 agcgaacatt tccgtaaaaa aacgtataac tgcacggatt ttgggacaat tatcaaacag
    28801 atatttgtcc cgattcccgt tttaccgaaa aaagaaaggg ggatcgattc ctgtgacaaa
    28861 agaagaaatt ttaacgggac ccggtgtcat aaagctcgtt agtcaataca tgggtcccga
    28921 acatgtagca tttgttgaga aagcatgcga atacgcgact gctgcacatg acggacagtt
    28981 tagaaaatca ggcgaaccct acattattca tcctatccaa gtcgcaggta tattagcaga
    29041 tttaaaaatg gatccccata cagtggctac aggcttctta catgatgttg ttgaagatac
    29101 agaaatcact ttagaagatc tgagagaaga atttggcgat gacgttgcta tgttagtgga
    29161 cggcgtaacc aaattaggga aaataaaata taaatcccac gaagagcagc tagcagaaaa
    29221 ccaccgaaag atgctgctag caatggctca agatttacga gttatcatgg tcaaattagc
    29281 tgatagacta cataacatgc gtacgttgaa gcacctgcga gaagataagc aaagaaggat
    29341 cgctcaggaa actttagaaa tctatgcgcc tcttgcacat cgtctaggga tcagccggat
    29401 caaatgggaa ttagaagata cagcacttcg ttatctaaat ccaaaacagt attaccgtat
    29461 cgtccacttg atgcagacga agagagaaga acgtgaaaaa tacgtaagtg gtactgttga
    29521 agatattcga atagcgacag aagagctggg gatttttgca gaaatctatg gacggccaaa
    29581 acacatttat tcaatctatc gtaaaatgaa agatcagaaa aagcagttca acgaaattta
    29641 tgacttgcta gctattcgag tgatcgtaga ttcaatcaag gattgttatg ctgtattagg
    29701 agcaatccac acaaaatgga aaccaatgcc cggcagattt aaggattata ttgccatgcc
    29761 aaaagctaat atgtaccaat ctttgcatac taccgtgatc ggtccggcag gaaatccggt
    29821 agaaattcaa atccgaacac aggaaatgca tgaaatcgct gaattcgggg ttgctgcaca
    29881 ctgggcctat aaagaaggaa aaaatgaaaa agtagaacca gatggtatga cgaaacaatt
    29941 aagctggttc catgagatac tcgaacttca agacgaaagc tatgatgctt ctgaatttat
    30001 ggaaggcgta aaaggagata tctttagtga taaagtctac gtcttcacac caaaaggaga
    30061 cgttactgag ttaccaaaag gatccggacc attggacttt gcatacagta tccatacaga
    30121 tatcggtaac aaaaccactg gtgcaaaagt aaatggcaaa atggtgcagc ttgattacaa
    30181 attgaaaaac ggagatatca ttgagatcat gacttctcca aattcatttg gcccaagtcg
    30241 cgactggttg aaattagttg ctactagcaa agcaagaaat aagatcaaac gtttcttcaa
    30301 agcccaagat cgagaagaaa atgtgatcaa aggccacgaa tccgtggtca aatgtattac
    30361 agatctagga tttacgccta aagatatttt gacgaagaac aaactgcaag aagcactcga
    30421 tcgttttaat tatcaaacag aagatgatct ctatgcagct gtagggtatg gagaagttag
    30481 ccccttgacg atggccaatc gtctgactga aaaagaacgt aaagaacaaa aaatcgagca
    30541 gcaaaagcaa gaagcagaag aaatcatgaa tcagccgaaa aaagaacctg acaaaatgaa
    30601 agtacgtcat gaaggtggcg ttgtcattca aggggtagaa aacttactaa ttcgtatcag
    30661 tcgctgctgt aatccgattc ctggtgatga tatcgttggt tatatcacta aaggcagagg
    30721 gatatccatt catcgtcgag attgtccgaa tgttcagcct gacaaaccaa atgtagcaga
    30781 acgtttgatt gaagtcgaat gggaagatac atcgaataca cgaaaagagt atgatgcaga
    30841 tttggaaatt tacggctata atcgttcagg cttattgaat gatgtacttc aaacagtcaa
    30901 tgcgctaacg aaaaatctca acagcgttga agcacggacg aataaagata aaatggcgac
    30961 gatccatttg acggttggta tccagaattt atcccaccta aagagtatcg tggataaaat
    31021 caaagcagta cctgatgtct acagtgtacg ccggacgaat ggataggagg acagatatga
    31081 gagcagttgt tcaacgggta agtaaagctg ctgtgtcagt cgatcagcaa gaaattggaa
    31141 aaattggcta cggcctactt gttttattag gcattcatga aaccgatacg caaaaagatg
    31201 tggactatct ggtaaaaaaa attgcacaaa tgcgtatttt tgaagatgaa caaggaaaaa
    31261 tgaatattag tgtagaagat gtgggaggac aaattttaag tatctctcaa ttcacactgt
    31321 ttgcggatac gaaaaaagga aatcgaccaa gctttgtgga agccgcacga ccagaaacag
    31381 ctattccttt atacgaatca ttcaatgaag gaatcagaag tcgcggcatc acagtcgcta
    31441 ccggacaatt cggagcagat atgtcggttt ctctcgtcaa tgatggacct gtaacgatca
    31501 ttattgattc taaaaacaaa taaagtaatc tggttttcac tagtaaaaac tatgaaacac
    31561 gtcgcttaag ttagactgat aaactctaac ttaagtggct tttttgtctg caaaaagcgc
    31621 ttcttttgtc agcagtcgga aaaaaagtta agcttaaatt atacaagtta ttttttatag
    31681 gaggttgctg aagatgaaag aagtaccagt agtagatatc aaaatcacag atttaaaaaa
    31741 attgtatcaa aaaggataca acatgttgga ggagttacgc catgaagcgg acgctcccgt
    31801 cgtcaaagca aagattttca ataaagaagc tatcacgatc tacggatcat cagcagcaaa
    31861 agtattttat gatccaagga atttcaaacg aaaaggtgct atgccaaaat tagtattgaa
    31921 gacacttttt ggtcaaggtg gagtacagac tttggatgga gctgctcatc accatcggaa
    31981 aaatattttt atggatctaa tgacaccgga aagaatggaa gattatcatc gtatcttaga
    32041 taagaatctg acacaagcac ttgaagcaca gcatggtcaa tttgaactat ttgatttaag
    32101 taagatggtt ttctttacat ctatttgcga gtgggcaggt atcaatttat cagcgatttc
    32161 taaagatgag gtcgaaaaat tagcagaata tcaaatctct atgatcagcg gaacgtttac
    32221 ttctccgatt gatcacataa aaggtgtcga gaatcgcaaa aaatcagaaa aatgggcgca
    32281 aggcttgatt gaagaagctc gacaaaatcc tgtggctgga aaagaaaatg tggcattata
    32341 tgcttttgcc aatgccacag atttagatgg tcagctttta cctctggaag tggcagctgt
    32401 tgaattattg aacatcatcc gtccaacggt tgctctaact gtgtgggcgg cattgatggg
    32461 acatgcactt ttcagtagac cagatttgta tcagcaatta aaaaatgact tttcgacgtt
    32521 acaagatccg tttattcaag aaatgagacg ctattatcct ttcttcccaa tgctacctgc
    32581 gatttcgctg aaagaagtag aagtagatgg ttatcgtata ccagaaggca gttgggttat
    32641 tttagattta tatggaacag accatgatga acgaacagtt gaagcgccag attcctttat
    32701 gatcaaacgg tatgtaggga aagcaaaaga tatttcctat aaagaagaat atgagatgat
    32761 tgcacaaggc gggggaaatt ttcgacaaat gcatcgctgt gcaggagaat ggatcactct
    32821 tcatagtcta cgtgtgtttt ccgatcagct agtaaataag tttgagttct ctgtacctga
    32881 acaagattgg acgattccat tcaatcagtt tccgacttat ccgaatagcc gagcgctact
    32941 ttacaagaat tagattagca taaacttatc gaaaaaaagg aagatcaaac agtgctgatc
    33001 ttcctttttt catattacta taaattgata aggtcaattg atcaaaatcc acgaaatgct
    33061 tcatcaatgg tttgataatc ttcattggat aaatgcaaat ctaaagcttg gacattgtca
    33121 gatacttgct ctggacgctt agctccaggt ataactacag acactcgtgg attcttcatg
    33181 taccaagcta aaacaagttg cgcaggagtg gcttggtatc ttttagcaat ggggcgaagt
    33241 acatcgacag cggttaaaat ttctctgaaa cgttctcctt ggaaatctgg atttcttttt
    33301 cgaggatctc cttcaccaaa ttgtttttcc tcaccaagtt catattttcc tgtcagcaaa
    33361 ccggaagcaa gaggaaaata gggaacaaaa ctgattttgt ttttttcaag ataagggaat
    33421 aattcttttt cagcctgccg atggatcaaa ctgtatttat cttctactac atcgacatat
    33481 ccatctgcat tcgcttcttt caattgttct aacgtgaagt ttgacacacc gactgctcgg
    33541 atttttcctg cttcttttaa ttcgtgcaat gtagcaactg attcattttt tggtgtgctt
    33601 tcatccggaa agtggatata aaaaatgtcg atgtaatcag tttgcagacg ttttagtgca
    33661 tcttccaccg attgtttgag aaactcagga gaattatcaa atgttcgccc tttatttggt
    33721 acatgagcag cttttgtagc gatgacgaca cgtgagcgat catattcttt ttccttcagt
    33781 acctcgccaa tcagttcttc ggaacggccg tttccgtaag cataagcggt atcgatcaaa
    33841 ttgatcccgc tattcaaagc tgtacgaact acttgttttc ctgtctcatc ttccaaaccg
    33901 tcaaaaagat tatgtcctcc aacggcattt gctcctaatc caagctgttc tgcgtagact
    33961 tgtgaatgtc cgattcttat ttctgccata gctgattcct cctttttgtc tagtctaaaa
    34021 caaaatacca aaaagctgaa ctaaaacgtt tttgtgaggg gaaatagtca gatatctctt
    34081 gactttgaga ctggttttta gtaggttata agtaatatcg aatgtcaatg aaagagcgag
    34141 taagtttttg aaactttaca agagaggatc atcgaggctg aaaatgatct aaagtttcat
    34201 tagcctaaag acactctgga gatggacgag tgaaaagccg tcccgcgtac gagcgttatt
    34261 cgtaagagga aagagaaatc tttcaaaatt aggtggtacc gtgtatgaat ttcattcacc
    34321 cttgttcatg atgaacaagg gtgatttttt tatttacata gctaagaaag ggagagaaaa
    34381 ctatgagttt ccaaagacca aaaggaacaa atgatatttt gccaggcgaa tcagaaaaat
    34441 ggcagtttgt cgaagaaaca gcacgtttat tattcagaga ttatcagtat aatgaaatgc
    34501 gtacaccgat ttttgaacac tatgaagtga tatcccgaag tgtaggggat acaacggata
    34561 tcgtttcaaa agagatgtat gatttttatg acaaagggga acgccatgtg acgcttcgac
    34621 cagaaggtac agcaccaatc gttcgttctt ttgttgagca taaattgttc ggcccagagt
    34681 atgcgaaacc gttcaaaact ttttatatgg gaccaatgtt tcgctacgaa cgccctcaaa
    34741 aaggacggtt acgtcaattc catcaaatcg gagtagaagc atttggatca gaaaaccctg
    34801 caactgatgt ggagagcatg gtgatggcac ttgatttctt caaacagttg gggatcacgc
    34861 aaatccggct agtcatcaat tcattaggtg ataaagaaac gcgtcaagcc tatcgtcaag
    34921 cattgatcga ctatctttta ccttttgaat cagagctgag tgaagactct aaacgccgcc
    34981 ttcatgaaaa tccattacgc gtattggaca gtaaagacaa acgagatcaa aaatttgtgg
    35041 cagaagctcc atctatctta gattatctaa gcgagcctgc tcaaaaacat tttgataccg
    35101 ttgtttccat gctggatgca ttggctgttc cttatgaaat cgacagtaat atggttcgcg
    35161 gactagatta ttacacacat acgatttttg aaatcatgag tgacgcacca aaaatggggg
    35221 cacaagcaac gatttgcgca ggcggacgtt atgacaatct agtggaagaa ctaggtggtc
    35281 cttcaacacc tggcttcgga tttgcaatgg gtatcgagcg attactcttg actatggagg
    35341 cagaggaagt agttatccct gcatttaatg agttggatgc ttacgtggtc gcactcggag
    35401 acgaaacaaa tatcgaagca ttgaaagtgg tccaagcgat tcgaaacttt ggtttttcag
    35461 ctgaccgcga cttcatgaac cgtaaagcaa aagcgcaatt taagacagca gataaagcga
    35521 atgccaaatt agtattggtg atcggtgaag atgagcttgc tagtcaaacg attaatgtca
    35581 aagcaatggc aagccgaaaa gaaaaaagtt ttccattaga agagatatat gaacattttg
    35641 atgaagtcta tgatgaaatg actttattcg agtaggagga aaaaaataaa tggcacaaag
    35701 aacgatctat tgcggattag tttccgaaca acaattagaa caaacagtta cattaaaagg
    35761 atgggtacaa aaaagacgag accttggggg agtaatcttt atcgacctac gtgatcgtga
    35821 aggaatcgtt caaatcgtct ttaatcctga aatcaacaaa gaagcatggg aaatcgcgga
    35881 taaatgtcgc agtgaatacg tgatcgaagt gactggggaa gttagaagac gcggagaaaa
    35941 tgcagtaaat cctaaaatgg ccactggtga attcgaagtg attgcaagtg aaatcgtgat
    36001 tttgaatcaa gcaaaaaccc cgccatttca aattgaagat gatcaagcaa tcagtgacga
    36061 aatacgtttg aaatatcgtt atttggatct gcgtcgtcca aaaatgacta aaaacatcaa
    36121 attaagaaat gcgacaacaa aagcgattcg tcattattta gatgataatg actttctcga
    36181 tatcgaaaca ccttacctag ggaaatctac accagaaggc gcaagagact acctagttcc
    36241 ttcacgtgtc catgctggac atttctatgc gttgccgcaa tctcctcaat tgtttaaaca
    36301 attgctgatg aatgctggat tcgaccgtta ttatcaaatc gttcgttgtt tccgcgatga
    36361 agatttacgt ggagaccgtc agcctgaatt tacgcaagta gatattgaaa caacgttctt
    36421 aacagcagaa gaaatccaaa cttacacaga gggattgatt gcgaaggtaa tgaaagaagt
    36481 tcgcggtatc gaagtcacat tgccatttcc tcgtatgacc tatgatgaag caatggctcg
    36541 ctacggcagc gacaaaccgg atacacgttt tgatatggaa ttgatcgatc taagcgatac
    36601 agtcaaagaa gtagaattca aagtcttcca aatggctctt gaaaacggcg gtgtcgtgaa
    36661 agcattgaat gctaaaggcg ctgcagatcg ctactcacga aaagacatgg atcagttagg
    36721 tcaatatgta ggacaattcg gagctaaggg gcttgcatgg ctgaaagtag aagaagacgg
    36781 attgaaaggt ccaattgcta aattcatggg agaagcaaca gaagcaatca ttaaagcaac
    36841 agatgcaaaa ccaggtgact tgctgatgtt tggcgcagat aaaagtgaaa tcgtcgctgc
    36901 tgcattaggt gctatccgga cacgtttagg aaaagaatta ggactaattg atgaatcgaa
    36961 attcaatttc ttatgggtaa ctgattggcc acaatttgaa ttttcagaag aagaaggtcg
    37021 atatgtctct gctcaccatc cattcacgat gccaaaagaa gaagatatcc cgttattggc
    37081 tgaagaccct gcaaaagtct atgcagaagc ttatgatatc gtcttgaacg gttatgaatt
    37141 aggcggaggt tctatccgta ttcatactcg tgagcttcaa gaaaaagtac tagaaactct
    37201 aggctttaca aaagaacaaa tggaagaaca atttggtttc ttgttaacag ctttagatta
    37261 cggattccct ccacatggcg gtattgcatt aggattggac cgttttgcaa tgttattagc
    37321 cggagaagat aatattcgtg aagtcattgc ctttcctaaa aacggaaaag cagcagatgt
    37381 gatgagtgat gcaccaagta tggtttcgcc tttacaattg ttcgaattga atattgatgt
    37441 aacgaatata gaagaatagc cgataaaaag gtaaaaagga tttgtcgaat acgcttcttt
    37501 tgacaacctt gatttaattg aaaaacactt tataatgaag ccataacata taaagcgggg
    37561 cttttccatg aaaaaaacat tcgaaaattg ggcttatcat gattacacga ccaagctttc
    37621 ggtatctatc gtttatgcgt tgatcgcatc agtcgcattg aatttctttt atcagccagg
    37681 acatatttat tcaagtggta ttactggtct agctcagatt ttgacgactt tgtcaaaatc
    37741 actgatagga ttcgaagtac cggtatcaac aactttgtat ttactgaata tacctttgtt
    37801 tgtattggcg tggttaaaaa tcgggaagaa gtttacaatt ttcactttta tcacagtcac
    37861 tttgacctcc ctttgtatcc atattttacc ggaaacagtc ctatctaccg atccaatcat
    37921 ctgtgcgttg tttggggggg ctgtgatggg actaggtatt gggctagcgt tgagaaacgg
    37981 cctatcatcg ggcggtctag atatcgtgag tatcacgatt cgtaaaaaaa caggaaagtc
    38041 gattggttct atttcgatct acttcaatgc attgatcgtt tttgttgccg gaattctatt
    38101 tggctggcaa tatatgtttt acagtacgtt gtctattttc gtcagtggaa aagtaactga
    38161 tgtcgtttat actaaacaaa agaagatgca ggtaatgatc gtaacgaaac atccagaaaa
    38221 agtagtaaat gaaatccaaa actgtatgcg tagagggatc acgatcatca atgaagcaga
    38281 aggtgcttat aagcatgata agcagacagt attgatgacg atcgttacac gatacgaact
    38341 gcctctactt agagaagcaa tgaaagcttc tgatccacaa gcttttgtaa gtattgcaga
    38401 aaacgtccag atacttggtc gtttctatga ggatggcatt tagatcgagt ttgacaaaaa
    38461 ttcatgaaat actgctgtag acagacgttc gtttgtctgc agtttttttc taccatagta
    38521 gaatcagcaa agaaaaactg cttttttatt tgaaattttc ttaaagttga aaaaaagcat
    38581 gtttattttt ttaaatttat gggatgatag aaaaaaatag tagagaaagg acacgattct
    38641 acttgctatt ttcacttaat acccgtatat tacgaatgaa aaaagtttat gaaaagttgg
    38701 aatattgaat ggaaaaacaa aaaaggttaa aaaacagccg ctacatcact gggtttgatg
    38761 ggctccgttc attagctgtg gtgggtgtta tcttctatca ccttctgcca actagtatga
    38821 aaggcggcta tttgggcgtt ccgatttttt tcgtcgtatc tggttatttg atcacggatc
    38881 tgctgcggca ggaatgggaa caaaatggaa aaatcaatat ttggcaattt tatgtcagaa
    38941 gaatgaagag actctatccg gcacttgttt ttatgcttgt gacagcttcg gcttatatca
    39001 ctttgtttca gcgagggctg ttaaacaatc ttcgaggagt tgtggtcagc agcttactgt
    39061 atgtcaacaa ctggtggcaa atcaataacg gtttatctta tttcgatcga tttgctaatg
    39121 agtcaccgtt tacgcatatc tggtctttag cagtagaagg tcaaaactat ttggtttggc
    39181 cgatcttgtt tgtattgttg atggtatttg ttcgtaaaag aaaatggatc ttttatactg
    39241 tgttaggtac atccttcctc tccgccatct tgatgatgat cttgtataca ccaggaggcg
    39301 atccaacccg tgtttattac ggtaccgaca ctcgtctgtt ttctatatgg ttgggaagtg
    39361 cgcttgcgtt tgtttggcca agtacgcgtt taaagaaaaa catcccgaaa caagcgaaaa
    39421 atattctgaa tatcgctgga ggaatctcgc ttttggcact gattctttgc ttcttctttt
    39481 tagatgatca ttacaatttc atctattacg gaggaatgct cctagtgagt ctgttatgcg
    39541 tagtgctagt tgcagtgaca gcacaccccg gagcaagttt gaatcgatgg ctgacaaatc
    39601 cagtatttac ttggattggc aaacgaagct atggcattta tctgtatcag tttccagtta
    39661 tgatttttta tgaagcaaag gtgactgata ttgccgatca tgttttgttg catactttag
    39721 tggaaatcac attgattcta atcatcagtg aactttctta tcgatatgtg gagcgtccgc
    39781 tagcacgttt tgattatggt cgtacattcc aagtgatgaa aggatggttc agcaaaccga
    39841 tcatcagtaa gagcaaacca tgggttgttc caggcacatt acttgtagca attgcactcg
    39901 ttggtttcgt tacggcaccg aaaaacgctg taacagccga tcaaaaacaa ctgcaggcaa
    39961 aaatcgctga aagtaaaaaa ttagcagaag aaaccagaaa caattcagaa aacaccacta
    40021 gttcggttga ccaagcgatc cttgataagt acgagttgtc caaggcacaa gggaataagg
    40081 cacaaaaatt agaactaacg gcatttggtg attcagttat gttggatgct gcaacggatc
    40141 tacaagaact ttatccaaaa gtagtggtag atggagatgt cggacgacaa ttatatgcca
    40201 gtgtaccgta cattgaaaaa ttaaaggaac aagatttgtt gaaagacact gttctgattg
    40261 gactaggaac gaatggctca ttctcggaag ctcagtttga tagtgtgatg gatgcaatcg
    40321 gtgatcgaaa agtatactgg gtcaatgtcc gagtaccgac gaagcgctgg caaaatgacg
    40381 tgaatgcgat gcttgaacag atgacgaaaa aatacgacaa tatgacgctg attgattggt
    40441 acagctacag taatgaccat agtgactggt tttatgatga tcaagtccat ccaaatcctg
    40501 aagggatgac acattacatt cacttagtca gcgaagcctt gctaggagga agttcaagta
    40561 cgaacagcag tgagacaaac gtgacagaat gatagctaca aaaaacttca ggcaaaaaca
    40621 tttgtttttg cctgaagttt ttttatattt accgtgtttt taacattcat atagattcag
    40681 gaagcaaaac ggttgagtgt gtctgtaaat aacgttcttt ttgttttcgg gtgagttttt
    40741 tgaatgcagc ttctgctttt gttgcttcgc ttcttgaaga aaatacttct gcatggatca
    40801 tcttaacagg tcttcttttt tgaagacgag tatatttagc accagtacca ttgttatgtt
    40861 cctctaatcg tctagaaaga tcggttgtat atccaccata aaatgtctga tctttacaga
    40921 ataatacgta aaaataatgt tcatttgcca tagagcatct cacgaatctc tgcagtgtat
    40981 tctccatcca tattgtaagt gatcaatggt ggagcgactt tgaatccgtc taatttgcct
    41041 tctttgattc cttcaactaa caaaatattt gcctcttttc caggttttgg gtaaacaaat
    41101 tggatccttt taggtgcaat tcgatagtct tccatgacct gaatgatttc taagaaccga
    41161 tcaggacgat ggaccatagt aaagcgtcca tttgttttta gtagcttact tgaaatatcg
    41221 atcacttctt ctaacgtcgt atgaatctca tgacgggcga ttgccaggtg tttattaggg
    41281 ttcgttttat ttgttggcag ccctttgaaa taaggtggat tacagacgac taagtcacaa
    41341 gaattatgtc gaacgacaga taagctgtct ttcagatcga tggtgtggac agtcatttgt
    41401 tcatttaaat ggttaagcgc gatactacgt cttgccatgt ctgctagacg aggttgaagt
    41461 tcgatagcgt cgatagccgc ctgggttcgt ttacttaaaa aaagtccaac tgcgccattt
    41521 cctgcacaaa ggtcaacaat cttccctttt ttaggtacac gggaaaagtg agcaagcagc
    41581 acagcatcta aagaaaaaga aaacactttg gaactttgga taatcttgat atcttctgcg
    41641 tataattggt cgattcgttc gtcttcgtga agcatggatt ccctccaaaa tgaaaaaaat
    41701 agtcgtctgt attatactat ttttattaaa aagaaccaag gtggcttgtc aaacctctat
    41761 tttttatgca tactatgacc ataagaaggg aaaggaagag actatgtttt atcgatttat
    41821 gcgcggggta gttcgagtac tattagcctt agttaatgga aatgcccgct atgagaataa
    41881 agaagtttta ccgcaagatg agaattacat actagttgct ccccatcgta cttggtggga
    41941 cccattatat ctggcggtag ctgcagcacc caaggaattc agctttatgg caaaagaaga
    42001 actgttcaaa aatccagttc tgcgatttat attaactcga tccaatgctt ttccagttaa
    42061 aagagaaaat ccaggaccaa gcgtcattaa aacacctgta aagattttga aatcgacaaa
    42121 tttgagtttg atcatgtttc ccagtgggac aagacatgct acagaactta aaggcggaat
    42181 ggcattgatt tccagaatgt ctaaagcgaa aatcgtgcca gctgtatatc aaggtccgtt
    42241 gacacttggg gatctgttca agcgtcagag agtgacagtg cgtttcggcg aaccaatcga
    42301 cttatcagat atcaagaaaa cggataaaga aggtatggcg gagatcgaac gacgaacaca
    42361 agcagctttt gacaaattgg acaaagaagt gaaccctgat ttcaaatatg aagtcaaaaa
    42421 aaaataaaat gataagttta tgcgtgggcg gtcttttgaa tctactgtcc tgttcagttc
    42481 agactttttc aatgaaaacg agggagagac gtcaagtctg ccctcttttt gcgttccaat
    42541 atgtaaaata gaagacaggt gtcatgtaat ggatcaaagt gttataataa ttagaaggac
    42601 attttttgag gtgattgaat gcgtttttta catacagccg attggcatat cggaaaaaaa
    42661 ctacaaggtt atgacttgat tgaagaccag cgtcatgtac tatcttctat actagatatc
    42721 gcgaaagaag aaaaagtaga ggcaatcgtg attgccggag acttatatga taggagtgtc
    42781 ccagctgttg atgccgtaga attatacaat gaattgatgg tcgattggaa tttaaaggaa
    42841 aagatcccga tttttgctat atcaggaaat cacgatagca gtacgagact ttcagcgggt
    42901 acgccatggt tcagttattc gaactatttt ttgcgaacac agctagcaga agcatttgaa
    42961 ccagtcgagt ttaaaaacgt acaatttttc tttcttcctt actttgaacc aattgcagcg
    43021 agattatatt ttgaagatga caatatccga acgatcaagc aagcggtagc caaggtgata
    43081 gataaaatgc aggagctatt tgattcagaa aaaaaacaag tattgattag ccattttttt
    43141 gtaatgggaa gtgtaaaatc tgattcggaa acgaaaatcg aagtaggcgg gttggattcg
    43201 atccctggtg aactacttac accgtttgat tatgtagcac taggacattt gcataatcaa
    43261 gctgctttga gacaggataa tgctcgctat agcggttccc cattgaaatt ctctttatct
    43321 gagatcaacc aaaaaaaagg cgtatggatc gttgacctga caacaggcct ggagttgaat
    43381 tttagggaga tcgaacccct gcatgatatt gaagaaataa cagcgagttt caaagaattg
    43441 ctggatcctg ccttttatca gtccgtcaat cgggagaatt acatctatat ctatctcact
    43501 gatagaacga tcatcccgaa tatgatgaac cagcttcggc aaatatatcc tcgtattttg
    43561 gggattgaac gggcaaacgg tcgagcagaa caaacaaagc gcaaacgaaa agtactagaa
    43621 atgaaagatc ctcaaaaatt agcagaagca ttttttagtg aaatgacgga agagcagcta
    43681 actgcaaacc aagaaaaatg gctcggagaa acgttgcaag aaatcatgga aatggaatga
    43741 tcaagaatga aaccgaaaaa attacaactt aaaaattttg gtccttttat caatgagacc
    43801 gtagattttt cacgtttgac agaagcaccg ctttttttga tcagcgggaa gacaggagct
    43861 ggcaaaacga cgatttttga tgggatcacc ttcgcacttt ttggtgaaac ttccggaaga
    43921 cttcgctctg gaaaagagat gcgttctttg tttgctacac cagacgaaga aacaagcgtg
    43981 actttttctt ttgaacatca gcagatgagc tatgaaatcg aacgaaaacc agaacaagtt
    44041 ttagcaaaac gaaaaggaaa cggaacaaaa aaacaggcag ccaaagtaac gttgaccatt
    44101 tttgatggga aaggaaaaga aataaaacaa atcacaaaac gtacagaagc agatcagttg
    44161 atcagagaac tgatgaatct cgatgcgaaa cagttttctc aaatcgtttt actaccccaa
    44221 ggagaattcc gtaatttttt ggtttcatca agcagtgata aagagactgt tttacgtcat
    44281 ttattcggta ctcaattttt ccaacagttc aatgagcgat taaaagaaaa agcaaaacaa
    44341 cagcaacaaa aattggatca tttagagcaa gagctgcatc ttctgcaaaa aaggtttgtt
    44401 ttagtcaaag aagaggaacc agcagcaggg tttgaagccg ttttagccca atggacaaac
    44461 catcaggcaa tactcgaaaa acaaatcacg gaagaaaaag aagctctaac ggaacttcag
    44521 gaaaaacaaa agcaactaga aaattcctac tatcgttttg aaagcttgaa aaaaagttat
    44581 gaagaaaaaa cgatattaat aaacaagcaa aaggaattag ctgctcaaaa agacgaaata
    44641 gaggaaaaga aacgatggat tgggtattat gaatttacgc aacgattagt cgaaccaatc
    44701 cagcgcctac aagaaataaa agttgaaaaa atagaactgt cacaaaaaag tagtgagcaa
    44761 acaaagaaac tagcgatgac tacgcagaat tatgaagagt ggcaaaaaaa tgaagcaaac
    44821 cgcgtacgcc gacaaacaaa aatcaaagaa gctgctcaaa aactgcaaaa caaacagatg
    44881 atggtgccaa ttgtcgaatc cttacgtagt aaaaaacaag acgcagaaca attaacaatt
    44941 tcattggata aagaagaatt gaatgccgag aagctgtatc aaaatctcgc tatctatcag
    45001 gaaagaactc gaattcttca agaaaattta ctggatcagg aaaaactgca agaagatcgt
    45061 cttgcttttg gacagttaaa atatctagat gaaaagcagc aagaaataga aaaacaaaaa
    45121 gcaaaagaca ctgggtcgct cgcacagata agagaagaac tcaaaaaagc aactgaagaa
    45181 ataacaaaaa atagtcagct attgaaagaa cagacagaac aagcagaaca gctgaaaagc
    45241 caatgggcaa agcagcaaat tgctcgtctt cagctttttt tgcgtccagg ggagccatgt
    45301 cttgtttgtg gatcaacgga acaccccata caacaattaa aacacgaaca gccaaataag
    45361 gaagatatct taaaagtaga acaacaactg gcagaagctg agtctgctgt agaaagaact
    45421 cagcaaatca ttgccaaaag tgaagcacat gaaaataatc ttcaacaaca attatcagat
    45481 ttagaacaaa aaatcatggc aacgaatata caattggctg aacaagaaga acacttgcaa
    45541 caggctttga aaaaggcatt tcctgagata acagaacaaa aaacaaaaac aactcttgta
    45601 gtaatcgaac aatctttaga tagacgaaaa agcaagatgg ccaatgaaag aatagagctg
    45661 gaagaaataa gacaacaagc agaaaagctc gaacaacaat tagctgtcta caaacagcag
    45721 cagacagaaa aaaataatca gctccagcaa ctgcaaggag aaatcaaaag tttagaacaa
    45781 caattgcaag atacggatag taactgttta gaagaggaga tacgacgact tgaaaaacag
    45841 ttagaagaag actcgaatca actagctgtt gagcagaaag caggagtgca attgttgcaa
    45901 gatcttcata cgcttcggac acagcttgat cttcaacatg aacaaataga aaaaatagat
    45961 gaaaaaatag aaaaggaaca agaaagaata gctaaaaagt tagaaagcca gtcgtatttt
    46021 tcaacaattg aagaaatctg tgcatttgca caacagcaag cacaatataa agaaaaccaa
    46081 cgtacagtta cttcttatca tgaagagcaa ttagtgacag ctgaccggct gcgtcaatta
    46141 gaaaatatcc agctaactga gacattccca gatacagaag agatcagcca acagttagaa
    46201 gaaataagac gtatgatcga aataaagcaa gaaaagatat acggtttaca agaacaaaaa
    46261 attagtaatc aaaaaatcta cgatgaatgt ctttcgattt atcacacaag ccagacgaaa
    46321 ttagatgagc ttacgcaatt acagcagctt tcgcaaacat tcaacggaga aaatcctaag
    46381 aagactagtt tggaacggta tgtgcttcag gtttatcttc aagaagtgtt acaagtagca
    46441 aatgatcatc tccaacgttt aacgaaaaat cgttatcaat ttgagctagc ggataccatt
    46501 ggcagttatc gagggaaaac cggattagaa atcaatatct atgatgatca ggcaggtatg
    46561 tctcgaagtg cgcataccct gtctggcggt gagagtttta ttgcagcact ttctttagca
    46621 ttagctcttg ccgatgtgat ccagacacaa gctggtggtg tagcaatcga tgcattgttt
    46681 attgatgaag gatttgggtc attagatgaa gaagctttgg aaatggcaat ggaagcttta
    46741 gaaacgatcg aaagtgaagg aagaatgatc ggcatcatta gtcatgtaag agaattgaaa
    46801 gaacgagtat tacaacaaat aagaattgat acagaaggta gcggacaaag caaaatacgc
    46861 tatcaacttg gataaaaagg gagattggta actatgaaat ggagtattcc agaaaaagtc
    46921 attgcacgtg gacgtcaata cttgcaagaa ggccgagtgt tatcggttgt ccctgaccaa
    46981 gaagaaaacg tgtggcatgc agaagtcttg ggcagtgaat tgtatatggt tgatttggat
    47041 ggtacagcaa aagagataga ttactgccaa tgtccctatt gggaggaaca taaatattgt
    47101 aagcatacag ttgctgtgga actttattta cggtcgaaaa atctatctcg tatcatgaaa
    47161 aaagaccagc caatgctaga accggtaaaa gcaacaagtg agggagaaat cattgcaaaa
    47221 ggtttttctc gtctaaaaac acaaaacaat cctcaagcgg tgcaaccttt gattattgaa
    47281 taccaagtag aaacgattga gactaatcag tatcacccag agctttctat tttggcactt
    47341 tacttgcgta tcggttcatt ggcatcaccg aaaaaaacat atatcgtaaa aaacatctat
    47401 gattttttac aagcttacac agaaaaagaa acttacactg ttaataaaca gtaccagttt
    47461 cgtctagata aacgggcgtt tcaaaaagaa gatcaaaaaa tactcacgca tcttgcagga
    47521 agtgctcaga cgcatcaatt attaggtacg aacggcttac aagtcaaagg aaaactagat
    47581 aaaagatatc tgcttctccc agtagaacaa gcccgcttcc tgttagagca gatgaatcag
    47641 actacacgtt tgcatctgca gataaatgaa caaaacgatc aaggaataca attttctgat
    47701 caaaacaaac ctttatcatt caaagtggaa aaatttggag aagattattt gctgcaagca
    47761 ttgaatgatt ttgatttttt ctttatgcat tatcagtggg gagttatcca agggacattt
    47821 tattctctga cgaaatatca acaaacggtt tatcaaactt ggaaacaatt gattcgccga
    47881 ttggagaaac cagaagtagt gttcaagaaa aaagaattac cgtctttgtt taaagagatt
    47941 atccctctgc tgtcagagat cggcgacgta gctattgccc ctgcagtaga agaggaagta
    48001 gcagatatcc ccgtcgaatt tattttcttt ttccgaaaag caaaaggaaa aatccaagct
    48061 cgagtcgatt tcgtctatgg tgaagtcatc tattcgactg acccgaagca tgaggtctcg
    48121 acagaacagg cttttcaggt gttacgtgac acagtagaag agaaacgagt acttgatcta
    48181 ttcagaatgt atcgctacca gaaaaatgaa actggttatg aacgtccatt gccaagcggg
    48241 gaagaactat atgccttttt ccgcacagaa ctatctgtct tccgtcagtt tggtgaagtc
    48301 agacttggaa gaaaattgcg agagttgtat ttagatgcgc ataaacatca accgaaaatc
    48361 gaagtaaccg agaccggttc atggctcgat attcgattcg atgtgacagg gatagaggag
    48421 caagaaatcg atcatgtcct gcaaagcttg ctgagaaatg atgcttttta tacattagaa
    48481 aatggtcagg ttctttcttt tgattcagaa gaatttcaac aaacaagcca agtgttgcaa
    48541 caattacggg aatcgatacg tacagaagaa ggaacgatcc atgtacctaa aaatcaagga
    48601 ttgatcattc aaaatcaatt ggaaaaaagc aatgctactt ttagtgaatc gttccaaacg
    48661 atggtacaag atctcattca tccagaaaga taccaggcac agcttcccaa aggattgaat
    48721 gcaacgatga gagattatca aaagcaaggt tttcgatggt tgaagatgtt aggtcattat
    48781 cagtttggcg gtattttagc cgatgaaatg ggacttggga aaacactaca gacaattgcc
    48841 tttttacttt cggaaaaaga agaacggaaa agcttctctg ctttgatcgt agcgcctgcg
    48901 agcttaattt acaactggca ggcagaggtg agaaaattcg ccccttcttt aagtatccaa
    48961 gtaatcaatg ggaataaaaa agaacgtgaa gaattactgg ctaaagacac agatatccga
    49021 gtgacttctt atgccagctt aagacaggat ctagctaatt atcagtcgca aaaaattgat
    49081 tatttgattc ttgatgaggc acaaatggtc aaaaatagca gtacgaaaac tgcacaagca
    49141 ttgagagaat tagctgtacc tcagcggttt gcactgagtg ggacaccaat cgagaataat
    49201 ttagaagaat tgtggtcatt gtttgcaacg attatgccag gatttttccc gactaaaaca
    49261 aaattcaggg aacttgccgc agaagaaatt gcgcaaatga ttcgaccgtt tattttacgg
    49321 cgagataaac aaactgtgtt gaaagacttg ccggaaaaaa cagagatgaa tctatattct
    49381 gcacttacag aagaacaaaa aactgtttat ttagcttact tgcgtcaaat gcgtgaagaa
    49441 atcacatcaa tggattctga agcattcaag aaaaaccgga tcgggatact tgctggtctg
    49501 acaagactgc gacaaatttg ctgcgatcct cgtctgttta ttgaagatta ccaaggcggt
    49561 tccggcaagc tcgaacaagt aaaagatcta ctggtagctg ccaaagagaa caaacgaaga
    49621 gttttgctgt tttctcaatt tacagggatg ctgactatct tgcaagaaga gttagccgag
    49681 ctagggatct cgacatttta tttaagaggc agtacgaaac cacaagatcg gttaagtatg
    49741 gtagacgcat ttaatgctgg cgaaaaagat gtttttctga tttccttaaa ggctggggga
    49801 actggattga atctcacggg agcagatacg gtcatcttgt atgacctttg gtggaatcct
    49861 gccattgaag aacaagctgc tggacgtgca catcggatcg gacaaaaaaa tgtggtagaa
    49921 gtgtggcgta tgatctctga aggaacgatt gaggaacgca tggattcact ccagcaagaa
    49981 aaacgcgaac tgttccaaaa agtgatccaa ggtaatgaag aacagttgac gaaactgact
    50041 gaagaagaca tccgcatgat tttaagtgta ggagaaatcg aggagtaaga gactcgaaaa
    50101 agcgttcagt aatgggtaag aaagcctttc taaagaagcg attgtttatt tgatttaaaa
    50161 aatagcaagg aggctgggat tccattttat cccagcttcc ttgctatcat ttttttattt
    50221 ctatttttaa aggttataca aagattgtct tcattgtctc taacagctga tcaaaagaag
    50281 aaatctcgat atcgcaaatt ggagtctgtt caattttgcg ttctcgatgg ttgaaccaaa
    50341 gcgcttgcca gttagcactt tttgcaccta gaacatcatt gtcgtaatta tcgccaacgt
    50401 ataaggtctc ttctggaagc atatgaaatt ctttctcagc cagctgaaaa atctcttttt
    50461 ctggtttttg gaaacctgtt gcttgagaaa tgagcatatt gcgggaagga atccaattgt
    50521 tcaattgaag ttgcttcagt ttttttgtct gatggtcagt cggcccattt gtgataattc
    50581 ctaatgggac attcttttcc ttcaagaaat cgagggtttt tttgacctcc tcatgcaagc
    50641 agatattatc aagttcttcc tcgtaaattt tttgaaataa gaggccatct tcttccgtaa
    50701 tatgagggta gtctaaatct tttagtgatt ggctgatccg atgtgctcgc atatattcta
    50761 gtgtccaatc tcctgccatt acttttggga agttttcatc actatgatgg cggaaccgga
    50821 tatatagtgg atgcatatct gcatctgaaa caagcggaac gactcttttg accgcgtttc
    50881 gaaatggctg ctgctgatca tacatcgtat catcaacatc aaacacaact gatttaatca
    50941 aaattttaca cgcctttcgg ttttcattga ttttttcaca aatccttgac tattgtaaca
    51001 gaaaaaattg tctaaaaaag ctgataaata aaggtttttc ttactatatt tctatttcct
    51061 tgacagaata aggtttttat atttcagttt ttttcactaa aaatcgtgaa acacgtcaat
    51121 aagtttgatg aacaaattaa tcattatggt agaagtcgat tgatttattt taatgcaatc
    51181 tctgtggaat tttcgaaaga tttatttttt taatacagtc tcaatataac tattcaaaca
    51241 taaaataacg ttatttgatt tatttgttta tagtaaattt tatttagtaa tcattgcttt
    51301 aaaatactac gcttatataa cagagaaaat aagcgttata ataaagaaca gtattattta
    51361 aaatgacaga aaacattact attaaagaaa taaaaaacat aaaaaatctt aagaaatcgg
    51421 tttcatttag cttttgagat aaaataatag tacagtttta tacgctgttt catatgttta
    51481 gaaaatttga attagatttg aaaactctaa tttttaaatg ataaaaaaag cgagaatgct
    51541 ggttttactg tatattaacc ttagataact ttcatttttt taaaaaaatt atgaaaaaaa
    51601 ggtagctatt taaaagttga ttatgtataa tagaaacaga tgggagatta ttatgatctt
    51661 ctgttacaaa aaagtgattc ttatcacgaa aaggagtggt gcacgttgtt aacattatat
    51721 acttccccaa gttgtacttc ctgcagaaag gctcgtgcgt ggttgcaaga acatcagatt
    51781 ccatttgttg aacgtaatat tttttcagaa ccattgaaca gttctgaatt aaaagcgatt
    51841 ttacaaatga cagaagacgg aacagaagaa atcatctcga cacgttccaa agtattccaa
    51901 aaactgaaca tggatttaga tgatctgccg ttacaagaat tattggaact tgtgcaaaac
    51961 aatcctggat tgcttcgccg tccaatcatg attgatgata aacgtctgca agttggtttt
    52021 aatgaagatg aaattcgtcg gttcctaccg cgcgatgtac gacaattaga attacgtcaa
    52081 gcacaattga tggctggatt atagaaggat aatacgaaac ttggaaaaga
//
LOCUS       DACDXO010000012        51635 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E23605_2017
            SAMN12639989-rid9881463.denovo.012, whole genome shotgun sequence.
ACCESSION   DACDXO010000012 DACDXO010000000
VERSION     DACDXO010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639989
            Sequence Read Archive: SRR10023389
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 51635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741914.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:42:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,972
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 33 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 17 of 73
            Pseudo Genes (multiple problems)  :: 10 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51635
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639989-rid9881463.denovo.012"
                     /isolate="E23605_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            119..1045
                     /gene="cysK"
                     /locus_tag="IWM35_000851"
     CDS             119..1045
                     /gene="cysK"
                     /locus_tag="IWM35_000851"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HAP9918721.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
     gene            1281..1727
                     /locus_tag="IWM35_000852"
     CDS             1281..1727
                     /locus_tag="IWM35_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAP9918722.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
     gene            2000..3352
                     /locus_tag="IWM35_000853"
     CDS             2000..3352
                     /locus_tag="IWM35_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase"
                     /protein_id="HAP9918723.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSNPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQAALGEIETAQ
                     "
     gene            3523..3810
                     /locus_tag="IWM35_000854"
     CDS             3523..3810
                     /locus_tag="IWM35_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9918724.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
     gene            4036..4479
                     /locus_tag="IWM35_000855"
     CDS             4036..4479
                     /locus_tag="IWM35_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP9918725.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
     gene            complement(4485..5669)
                     /locus_tag="IWM35_000856"
     CDS             complement(4485..5669)
                     /locus_tag="IWM35_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HAP9918726.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            5937..6635
                     /locus_tag="IWM35_000857"
     CDS             5937..6635
                     /locus_tag="IWM35_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HAP9918727.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            6642..7319
                     /gene="nth"
                     /locus_tag="IWM35_000858"
     CDS             6642..7319
                     /gene="nth"
                     /locus_tag="IWM35_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HAP9918728.1"
                     /translation="MLSKQKTMEALETMYGMFPEAHGELKHNNPFELLIAVILSAQAT
                     DVSVNKATPDLFASFPTPDALAEASIDEIILKIKTIGLYRNKAKNIKACAQQLIERFD
                     GQVPTSREELMSLPGVGRKTANVVLGDAFGIPAIAVDTHVERVSKRLRICKLDATVME
                     VEETLMRKVPQELWVKTHHTLIFFGRYHCTARNPKCEVCPLLSICQDGKNRMRLKEKA
                     LKKKPRL"
     gene            complement(7445..7978)
                     /locus_tag="IWM35_000859"
     CDS             complement(7445..7978)
                     /locus_tag="IWM35_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9918729.1"
                     /translation="MVLNNFLKEGFILSYLIVCLIIIICLHHLFLSKTIPRYSKNKKA
                     EKFCLLLNKFTLIAPILAFIIFSVLLSTTLKGKFMERSSHAMILTFLWLLFTRIYIFL
                     MSLKPPKSISLCLVINGIFLLSLIIFITPLDRYVTYLYNPLEYWTYFIGILEGIIFYI
                     GYFPNKNNNFYFYRNKL"
     gene            complement(8275..10656)
                     /locus_tag="IWM35_000860"
     CDS             complement(8275..10656)
                     /locus_tag="IWM35_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HAP9918730.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(10646..11293)
                     /gene="recU"
                     /locus_tag="IWM35_000861"
     CDS             complement(10646..11293)
                     /gene="recU"
                     /locus_tag="IWM35_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HAP9918731.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            11357..11896
                     /locus_tag="IWM35_000862"
     CDS             11357..11896
                     /locus_tag="IWM35_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HAP9918732.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            11986..12405
                     /gene="gpsB"
                     /locus_tag="IWM35_000863"
     CDS             11986..12405
                     /gene="gpsB"
                     /locus_tag="IWM35_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HAP9918733.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYEAYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            12427..12794
                     /gene="rnpB"
                     /locus_tag="IWM35_000864"
     ncRNA           12427..12794
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="IWM35_000864"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            13003..14169
                     /locus_tag="IWM35_000865"
     CDS             13003..14169
                     /locus_tag="IWM35_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HAP9918734.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            14215..15711
                     /locus_tag="IWM35_000866"
     CDS             14215..15711
                     /locus_tag="IWM35_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HAP9918735.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            15792..16475
                     /locus_tag="IWM35_000867"
     CDS             15792..16475
                     /locus_tag="IWM35_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP9918736.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
     gene            17120..18310
                     /locus_tag="IWM35_000868"
     CDS             17120..18310
                     /locus_tag="IWM35_000868"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HAP9918737.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
     gene            18412..19365
                     /locus_tag="IWM35_000869"
     CDS             18412..19365
                     /locus_tag="IWM35_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HAP9918738.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            19434..20783
                     /locus_tag="IWM35_000870"
     CDS             19434..20783
                     /locus_tag="IWM35_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP9918739.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(20966..21289)
                     /locus_tag="IWM35_000871"
     CDS             complement(20966..21289)
                     /locus_tag="IWM35_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9918740.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            21418..22581
                     /locus_tag="IWM35_000872"
     CDS             21418..22581
                     /locus_tag="IWM35_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9918741.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            22651..24000
                     /locus_tag="IWM35_000873"
     CDS             22651..24000
                     /locus_tag="IWM35_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAP9918742.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(24045..24476)
                     /locus_tag="IWM35_000874"
     CDS             complement(24045..24476)
                     /locus_tag="IWM35_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9918743.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            24864..27506
                     /gene="alaS"
                     /locus_tag="IWM35_000875"
     CDS             24864..27506
                     /gene="alaS"
                     /locus_tag="IWM35_000875"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HAP9918744.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            27706..28302
                     /locus_tag="IWM35_000876"
     CDS             27706..28302
                     /locus_tag="IWM35_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP9918745.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            28424..29572
                     /locus_tag="IWM35_000877"
     CDS             28424..29572
                     /locus_tag="IWM35_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HAP9918746.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(29610..30023)
                     /locus_tag="IWM35_000878"
     CDS             complement(29610..30023)
                     /locus_tag="IWM35_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HAP9918747.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
     gene            30102..30533
                     /locus_tag="IWM35_000879"
     CDS             30102..30533
                     /locus_tag="IWM35_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HAP9918748.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            complement(30632..30865)
                     /locus_tag="IWM35_000880"
     CDS             complement(30632..30865)
                     /locus_tag="IWM35_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HAP9918749.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            31048..32718
                     /locus_tag="IWM35_000881"
     CDS             31048..32718
                     /locus_tag="IWM35_000881"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HAP9918750.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            32865..33251
                     /locus_tag="IWM35_000882"
     CDS             32865..33251
                     /locus_tag="IWM35_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9918751.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            33238..33936
                     /locus_tag="IWM35_000883"
     CDS             33238..33936
                     /locus_tag="IWM35_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAP9918752.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            33941..34405
                     /locus_tag="IWM35_000884"
     CDS             33941..34405
                     /locus_tag="IWM35_000884"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HAP9918753.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            34402..35313
                     /locus_tag="IWM35_000885"
     CDS             34402..35313
                     /locus_tag="IWM35_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAP9918754.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            35548..36087
                     /gene="pyrR"
                     /locus_tag="IWM35_000886"
     CDS             35548..36087
                     /gene="pyrR"
                     /locus_tag="IWM35_000886"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015694870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HAP9918755.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            36101..37384
                     /locus_tag="IWM35_000887"
     CDS             36101..37384
                     /locus_tag="IWM35_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HAP9918756.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            37397..38323
                     /locus_tag="IWM35_000888"
     CDS             37397..38323
                     /locus_tag="IWM35_000888"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HAP9918757.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            38342..39622
                     /locus_tag="IWM35_000889"
     CDS             38342..39622
                     /locus_tag="IWM35_000889"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HAP9918758.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            39627..40703
                     /gene="carA"
                     /locus_tag="IWM35_000890"
     CDS             39627..40703
                     /gene="carA"
                     /locus_tag="IWM35_000890"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010744830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HAP9918759.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            40708..43893
                     /gene="carB"
                     /locus_tag="IWM35_000891"
     CDS             40708..43893
                     /gene="carB"
                     /locus_tag="IWM35_000891"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HAP9918760.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            43947..44723
                     /locus_tag="IWM35_000892"
     CDS             43947..44723
                     /locus_tag="IWM35_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HAP9918761.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            44720..45652
                     /locus_tag="IWM35_000893"
     CDS             44720..45652
                     /locus_tag="IWM35_000893"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HAP9918762.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            45649..46365
                     /gene="pyrF"
                     /locus_tag="IWM35_000894"
     CDS             45649..46365
                     /gene="pyrF"
                     /locus_tag="IWM35_000894"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HAP9918763.1"
                     /translation="MNQLDQRPIIALDFSTRQEVEDFLRFFPKEEKLFVKIGMELFYQ
                     EGPEIVRYLKEAGHNVFLDLKLHDIPNTVEKAMRGLAKLGVDLTCVHAAGGIRMMEAA
                     MRGLEEGTPEGGKRPLLLAITQLTSTSEEEMHADQLIEVSLEKSVIHYASCAKKAGLD
                     GVVSSAWEVEAIKEMAGDEFVCLTPGIRPEGTVAGDQTRVVTPSQAKKIGSTFIVIGR
                     PITQSTNPYKAYQTIQTEWS"
     gene            46372..47061
                     /locus_tag="IWM35_000895"
     CDS             46372..47061
                     /locus_tag="IWM35_000895"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HAP9918764.1"
                     /translation="MNVEQSIAKDLLEIEAVFLNPSNPFTWASGIKSPIYCDNRITMS
                     YPKVRKEIAKGLASKIKEAYPEVQVIAGTATAGIPHAAWVAEILDLPMVYIRSKAKDH
                     GKGNQIEGRIFEGQKMVVIEDLISTGGSVLEAAEAAKREGADILGVAAIFTYELPKGK
                     ANFEKAEIPLMTLTNYSVLIEAALEDRYIDEQELTLLKEWKKDPENWQIEARLMKIEK
                     SDLCLQNHQLE"
     gene            47034..47303
                     /locus_tag="IWM35_000896"
     CDS             47034..47303
                     /locus_tag="IWM35_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9918765.1"
                     /translation="MSPESSIGIAKIQKLIARSIFSSGMKRNFYRDQFLIFLKFIFLR
                     LVSRKKRIISSLFSIIYRFSLFFGKGAVSKMVKSGNVANISYLMI"
     gene            47341..47982
                     /locus_tag="IWM35_000897"
     CDS             47341..47982
                     /locus_tag="IWM35_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP9918766.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
     gene            48013..48717
                     /locus_tag="IWM35_000898"
     CDS             48013..48717
                     /locus_tag="IWM35_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HAP9918767.1"
                     /translation="MKKNMDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKG
                     EETKIEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSE
                     HIINGKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVN
                     PDVFFPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPI
                     QPLNGREIYYFEEFEQ"
     gene            48714..49616
                     /locus_tag="IWM35_000899"
     CDS             48714..49616
                     /locus_tag="IWM35_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP9918768.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(49774..51480)
                     /locus_tag="IWM35_000900"
     CDS             complement(49774..51480)
                     /locus_tag="IWM35_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin/fibrinogen-binding protein"
                     /protein_id="HAP9918769.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
BASE COUNT        16894 a         8983 c        11096 g        14662 t
ORIGIN      
        1 attaccatat atggtatatc tttgtctttt ttttgctagt caaagacgaa tcactcgcca
       61 taaatgaaca gattgtatta tgataagtaa gaagagcaaa gacaaggagg aattgaagat
      121 gacagctatc tataactcag tcaccgaatt gattggcaaa acgccaatcg tcaaattaaa
      181 taaaatcgta ccagaagatt cagcagatgt ttttgtaaag cttgagtttt ttaatccagg
      241 aggaagtgta aaagatcgta ttgctttaag tatgatagaa aaagcagaac acgatggtct
      301 actgaaacca ggtgatacta tcattgaacc tacttctgga aatacaggaa tcggattgtc
      361 gatggtagga gttgcaaaag gatataaagt aatcatcgtg atgcctgaga cgatgagtat
      421 cgaaagacgt ttattgatga aagggtacgg cgcagagttg attttgactc ctggtgcgga
      481 tggtatttct ggatcaatca gagaagcaga acgtttggca aaagaaaacg gctacttttt
      541 accattacaa tttgaaaatg aagcaaatcc tcttgtgcat gagaagacga ctggtcctga
      601 gatacatcaa gcatttggag taaatggctt ggacgcgttt gttgcaggta tcggtacagg
      661 aggaacgatc actggcgctg gccgagaatt aaaacgtgtg tatccgaaaa tcgaattaat
      721 cggggtagaa ccagcagaat ctgctatttt agaagggaaa gaagcgggac ctcacaaaat
      781 ccaaggaatc ggtacaggtt ttgttcctaa aacactggat acttctgtgt atgataaagt
      841 cctttctatt agtggagacg aagcgatgga aacagctaga gaagttgggc gaaaagaagg
      901 gattttagta ggcatctcat ctggcgctgc aattgctgcc gcgctaaaag tcgcaaaaga
      961 attaggaaaa ggcaagaagg tattagctgt ggttcccgac aatggtgaac gttatttatc
     1021 tacagccctt taccaagaga tataaggcac ttggtttttc tccagatagg tttatgtaaa
     1081 aaacttttaa agatgaaaag aacacacaaa aatttatgat gagaaaaatt atttgaatag
     1141 agacgtagaa tagtgaacgc gactgtttta cgtctttttt cattaaaata caataaaatt
     1201 gaattttctt ttcatgttaa gtggatttta aaatgtaaaa atagtatgat aagacgagaa
     1261 taacaacgtg gggtgtttac atggaaaata agttagtcga agaggttatc gaaaagttaa
     1321 aaaaagagaa cattcgaatc acacctcaac ggtacgcggt tttagaatat ttgattgaac
     1381 atcattccca tccaacggct gatgagattt ataaagcact tgaacatcgg tttccaaata
     1441 tgagtgttgc gactgtttac aataaccttc gcttatttac ttcgattgga tttgttcaag
     1501 aaatgaagta cggcgatgct tctagccgat ttgatttcag ttcaaagcgt cattatcatg
     1561 taatttgtga aaactgcggt aagattgttg attttcatta cccagggctt gaagatatag
     1621 aaatggctgc aagcagactg actgattttg agattaatga acatcggttg gaattgtatg
     1681 gactctgtcc ggattgtaaa gagtcagcaa aaaaggcgca aatgtaaaaa cagtttctac
     1741 aagtgttata cctaaaaaaa gctagctgtg taaaaaaaac gctagctttt ttattttttt
     1801 gtcttttatt cacaatctta gatataaaag tgaatattat taaaataaga atcttgtaga
     1861 tattgggttc ttatgcttga aaaggaatga aactacaaac aaacctttgt gaattcacaa
     1921 gcattaaact gttgtatccg ttttatttct gttatagtag ttttgtcgaa ggacaaaatg
     1981 ttaatcggag ggaccataaa tgaaagtagt agtaattggc tgtactcatg caggtactgc
     2041 agctgtaaaa agtattttaa aaaatcatcc agaagcagaa gtgacagtat atgaaagaaa
     2101 tgacaatatt tctttcttat cttgcggtat cgcactttat gtcggcggag tagtaaagga
     2161 cccagcaggc ttgttctact caaatcctga agaattatct tcattaggag caacagttaa
     2221 aatggaacac gatgtaacag acgtcgatac agaaaataaa acagtaacgg caaaagatct
     2281 gaaaacagga gaaacagaaa cggtttctta tgataaatta gtgatgacaa ctggttcatg
     2341 gcctattatc ccaccgatta gtggaatcga tgcgaaaaat gtactcctat gcaaaaacta
     2401 caatcaagca aatgaaatta ttgcacaagc gaaagaagca aaacgtgtag taattgttgg
     2461 tggtggatat atcggtattg aattagtaga agcttttgtc gaatctggaa aacaagtgac
     2521 attgattgat ggattagatc gcatcttgaa taaatatcta gataaaccat tcacagatat
     2581 cttagaaaaa gaattgacag accgcggtgt tactcttgcg ctaggcgaaa atgttactga
     2641 atttatcaca gatgaagaag gaaaagtgaa acaagtagct acaccaacag atacatttga
     2701 tacagatatg gtcattttat gtgtgggctt ccgtccaaat acaaaactga tcgaaggaaa
     2761 agtagataca ctgccaaacg gggcaatcaa agtgaatgaa tacatgcaga caagtaatcc
     2821 agacattttt gcagctggag actctgcagt agtcaactac aatccaagtg gcacgcaaaa
     2881 ctatattcct ttggcaacaa atgccgttcg tcaaggtttg cttgtgggaa ataacttgac
     2941 agatcataaa atggcgtatc gtggtactca aggaacttca ggactttacc tatttggctg
     3001 gacgatcggt tcgacaggtg tgaccaaaga aagcgcaact ttgaacgatc tagaagtcca
     3061 agcgactgta tttgaagata attatcgtcc agagttcatg ccaactactg aaaaagtaat
     3121 gatggagcta gtctatgaaa aaggaacgaa tcgtatcgta ggtgctcagt tcatgtccaa
     3181 atacgacatt acacaatcag caaataccat gtctttagct gttcaaaata aaatgactgt
     3241 agaagatctc gcactttcag atttcttctt ccaaccacat tttgaccgcc catggaatta
     3301 cttgaaccta cttgcacaag cagcgctagg cgaaatcgaa actgcacaat aaagaaaaag
     3361 ggttgtgaca caattttgtc acgaccctct gtcttttttt gtgaaaagta caattagttg
     3421 tctttcttcg aattttttgt cacaataagc agcaaagata aagctgttac ataaaagaac
     3481 aagcagcttt atttgatttt gaaaagaaag gtgagaaaga agatgaataa agaatatttt
     3541 gtccaacaag atgcattttt agaagattat aaaagtgctt cgaagaaatt aacgatcaat
     3601 attgatgagt tggcagatca agtactctct cagttgaatg aaacgcaaga ggaatattac
     3661 acgatgccag caaatgacgc caaagataat gtttctcatc gttttccttt cagtagacgc
     3721 atctactcag atgaaaatga cggcacattg aatacagagt acacatatga aggtcagcca
     3781 tatgtgctag aaactgatga tgatctttaa agacagtttt tgaaaaggcc gctcatcctt
     3841 aagtgatgac aaaaaatcat cacaattacc gatgactgtc tctttactat cttttaatca
     3901 gaaaaagggg ttatgacaag ctttgtcaac cccttttttt cttggtttca gattgggttt
     3961 tcctacaaaa tatttttatc tttgttgaat aggaaacaaa aaaacaatat aatgaaagaa
     4021 aaagaaggtg tagaaatgga taaaagacaa ctgctaatag aagaatatct ggatttagct
     4081 aatgagatta ttcatcgtaa taaaccagaa atggaaaaag aatttgcagg atttactcta
     4141 aatgaactag aagtgattga acatatcggg aaaatccctg atcctaacgt gacaaaatta
     4201 gcgaatgcta gttacatgac tagaggagcc atcagtaagc taacaaaaaa actgatagct
     4261 agagggatca taaacactta tcaaagcgaa gagaataaaa aagaaatcta ttttcaattg
     4321 acgccaatcg gacaaaaaat aaggaataga cacgagcaac tccatcaaaa atgggtacag
     4381 cgagatgcta ctgtatttga aaatatgtca aaagaagaat ttgacacagt atttcgtttt
     4441 atcggtcgat acagagaatt tatacgaagt agcaaatgaa gctactattt tttattgtag
     4501 tgataatagg caaaaatcat aagcaaaaat ccgaataccg tcggaagaaa tggtgctgca
     4561 gcataaagct gtcctgcgat tagcgggccg atgacgcgcg atagagattg tatactttgg
     4621 gtaccgccca ttacaagacc ttgctggtta tctgagacag aattggatag ttggccattg
     4681 aatacaggag taaaaataga atcaccgaaa ccataacaga taatcccaaa taaaaacaaa
     4741 atcggcagta gaagaattcc agataatgtg atgaatagat aggcgaggat ttcactgaac
     4801 atccctattc ttgtgatctg ctgttctgaa aattttttga gcaatcgagg cataatgaac
     4861 aattgcgaga cagtatccaa gattccaatc aaagagaagc ttaaaccaac caacccagcc
     4921 ttccactgaa aggcgtcaat cgaaaattga gaaaagatag attgaagaga gccagctgcc
     4981 aaccaaacag caaaacctgc tgtcaataaa ggaataacgg atgaaatctt gaataattgt
     5041 tgtaattggt gaaaaggtct tacatgggag aaacttaaat ctactgaacg tttttttatt
     5101 ggaaggcttt ctagcataaa tgtgtagcca taaacggcat ttaagaaagt aaagagtgct
     5161 ccaataaata taggaacaga attccccaac tcagctaata gaccccctat aataggaccc
     5221 agcgttgttc ctataccgac taaagcaccc atccatccaa aaactttggt tctttcatta
     5281 ggttccgtga tgtcggcaaa gtaagcatat aaggtggaga tttctcctgc cgtcaatcct
     5341 tcaatgatac gaccaaggaa caacatccat atcgaatttc ctaaaccaaa aagcaaatat
     5401 ccgatacttg atcctaataa actgataata aggatcggat gacgcccaaa atgatcactt
     5461 aagctaccga gaatgggggc agataagaag gcagctagag cataaacaga catgagcaaa
     5521 gtgatataga aagcttgctg atgtatggag tgagaataag gcagcgttaa aaacggaagt
     5581 acaggactga cgatagtaaa tcctaatcca gtgaataaga cagagacgta tccaaaaact
     5641 ttagaatgtt tatttgtgaa actgtacatg taaaaactcc tttatgcaat aatgttaact
     5701 tggaaacaaa aatagtgtat agtaattttg tttccttgtc aacattctaa ggaacagcga
     5761 aatctactta atttatttat tccgaaaagt gaagatacgc atcaaaaaca tgaacaaaca
     5821 aaaatcaagt agacgacatt gtaaatctga ccgatcagtg aagtaaatag aaaagaaacc
     5881 tgttcttgct tcccatgttt tttttacgta tactgtgatt gagcggagga gaaatcatgt
     5941 tgaatttaga aaattactta agcgctggac aaacagtcat atctaatctt ttacttaaaa
     6001 attatcatag gatcggtcta caatctgaag aatttttatt tgttctacag ttgcatatgg
     6061 cacagcttga aggagatact ttccctgatt tgcagatgat tgctatggat atgggaatca
     6121 aacaagatca gatttttcaa atactggatc gtctcgtaac aaatggattt atcaagatag
     6181 aaacgacgat tgtcaacggt aaaaaagcag atcgatataa tctttacccc atctatgatc
     6241 tattaggtga gtatttaaaa acacaagaaa aaaaatacga agaaaaaaca caagaaaaag
     6301 aaatccagtc agtgtatcaa ttatttgaac aagaatttgg acgcccgctt tcttcgatcg
     6361 agttccagcg gatcggacaa tggctagaag aagaccatta tcagccggaa attttgaagc
     6421 tagcactgcg ggaagccgta ttaaatcagg cttatagttt taattacgtt gaccgcattt
     6481 tgctatcgtg ggaaagaaaa aatctacgta caaaacaaca agtggaagaa gaccaaaaac
     6541 gaagaaaaca gcagcttctt caaagagaag tagaacagtc agctcaacaa gaagaattgc
     6601 cgaaagtctc actgaaaaat tggttggagg agtagcggaa tatgttaagc aaacaaaaaa
     6661 caatggaagc tctagaaacg atgtatggaa tgtttcccga agcacatgga gaactcaagc
     6721 acaataatcc atttgagtta ttgattgcag tgatcttaag tgcccaggcg acagatgtat
     6781 cagtgaataa agcaacgcca gatttgtttg cctcttttcc gacacccgat gcgttagcag
     6841 aagcttcaat cgatgaaatc attcttaaaa taaaaacgat tggtttgtat cgaaataaag
     6901 caaaaaacat caaagcgtgt gcacagcagc tgattgaacg tttcgatggg caagtgccaa
     6961 cgtcaagaga agaattgatg tctcttcctg gtgtaggcag aaagacagca aatgtcgtat
     7021 tgggggatgc attcgggata ccagcaatcg cagtagatac ccacgtggaa cgtgtctcaa
     7081 aacgcctgag gatctgcaaa ctagatgcta cggtcatgga agtagaagaa acactgatgc
     7141 ggaaagtacc acaagaatta tgggtaaaga cacatcatac actgattttt tttggtagat
     7201 atcattgcac ggcaagaaat cccaagtgcg aggtatgtcc tttgctatca atatgtcaag
     7261 atgggaaaaa tcggatgcgt ctaaaagaaa aggcgctaaa aaagaaacca cgcctgtaga
     7321 cgtggtttct ttaattgcaa ataaatgtat gaattatctt gttattatgg agaaaagctg
     7381 tttcgtttat cttttttgca tgaaaaagta aatgtacata ctcagtagct aggatggtaa
     7441 cttattacaa tttattgcga taaaaataaa agttgttatt tttattggga aaatacccga
     7501 tgtagaaaat aatgccttcc aatataccta taaaataagt ccagtattcc aaaggattat
     7561 ataaataagt gacgtatcta tctaaaggtg taataaaaat gataagtgat aataaaaaaa
     7621 taccatttat aactaaacat aaactaatgg atttgggcgg tttcaggctc atcagaaaaa
     7681 tatagattct ggtgaacaac aaccataaaa aagtgagaat cattgcatga gagcttcttt
     7741 ccataaattt tcctttaaga gtagtgctta aaagtacaga aaaaataata aatgcaagga
     7801 taggagcaat taaagtaaat ttatttaata ataaacaaaa tttttcagct tttttattct
     7861 tggaataacg aggtattgtt ttcgataaaa aaagatggtg cagacaaatt atgataatta
     7921 agcatactat cagataactc aaaatgaagc cttctttcaa aaaattattc aaaaccatat
     7981 attgtatttt ttatttgata ataacaatgg gattattaag aagtggcact aaataaaaga
     8041 ctagtgtgta tttagccgat ttaattataa acaagtaaat ggataaagta tatgtaatga
     8101 taaaagaaaa atggattttc taatttttaa gctaaatgtt agatcacaca tgtatatgct
     8161 ggatagtctt ataaaaaatc atgtaataca gcttggtaca tgatttttat ttgtagctgt
     8221 tttttacata gacacaaaaa agaaaccacg cttgtagacg tggtttcttt tttactatct
     8281 gtttccagaa gctgaggtgc tgctggatgg tgtatggtca tttgcagcat ccgctccgct
     8341 agttgctggc tcactggaag aagcaggagg agcagatgaa gacggtgtcg agctttcttc
     8401 ggatgaagaa gaaggaaccg ttgttgaaga cgctggctcg cttgtttctg ctgtcgtgct
     8461 ttcttttgag gactcagcag ttgattcgct ttgactagta gagctagagc tttgagtcgt
     8521 tgtctctgtt gttgagcttc caggagtctg gacatagctg gaagatggaa tagttgttga
     8581 tggtgtaatt gcattcgatc tagctgtata ttgatctttg tagtacagtt caccgcccac
     8641 acgaattaat ccgcttggca tctcccagtc tgtatttgtc acgttagctg aaacatactg
     8701 cataagctcg cgataaacat cagaggctac gtgtgacgat tctgaagtga caggtgtcat
     8761 ttttttattg taacctgtcc acacggaaat cgaataattt ggcgtatagc ccgcaaatag
     8821 gatatctgga tacaccccag aagaaatacc aagttttgca tactcgtcat ctgtgtaatt
     8881 ggatgttccg gtcttaccgg cttgatataa cccagcgatt tgcgcattcg taccagttcc
     8941 ttcagtgatc gtatctttta aaatgtctgt gatcatataa gctgtttctg gagacatggc
     9001 tgtttttcca tctggctcat aagtttcttc tgttccgtct tggaagacga ttttattaac
     9061 atattgaggt ttgtaataag tccctccatt ggcgaaagcc gcataggctg ctgccatttt
     9121 taaggaagaa gctccgtatt ttgtcccatc ttgttcttcc gtattactag agatcgcatt
     9181 cgattgatgg atcgtactgt attcgatccc aagatttttt aagaaagaag ccactttatc
     9241 tgaaccgact tcattgaata atttgactgc tggaacatta cgggataagt aaagtgcttg
     9301 gcgtaaagta atggtgccca tatattgatt atcccagttt cctacaggag tcgaagtccc
     9361 ttcatagtta tacggtgcgt cagtaatagt ttttccagta gaatatttca aatattcaaa
     9421 agctggtccg taatctgtga caggtttcat cgtagaacca aaatcacggg aagtattcac
     9481 tgctaagttg ttgcctaaag ttacgtcttc tgcgatgtga cgtccgccaa tctgggcttt
     9541 tactttcccg gtattggtgt cgataagggt ggaggcaact tgcatctcgt cgtctgggta
     9601 agagacatac tgatccgtgt tgacgatatc atatagtttc ttttgggcgt ccagatccaa
     9661 attcgtataa atctctaaac cgtctgtgta gacattctta tctgtttttt cttgtacttc
     9721 attgatgact tctttgacat aattatcgac gatcttagta ttgtcatcgg attgagttag
     9781 ttcttgcaga ccatccgtta ccggtacatt aacagcttga tcatattctg tttgagagat
     9841 tttttcgttt tgaagcatcg tatacaaaac agtatctcgt cttttcttcg cctgatctgg
     9901 ataaacataa ggatcatagg cggaaggggc ttgaggcatc ccagcaagca atgctgtttg
     9961 cggtaaagaa agttctgata atttcttacc aaagtacatc tcagaagctg tctccatccc
    10021 gtacaagccg tttgacatat acactttatt tacgtagtag gtcaagatct cttgtttcga
    10081 ttttttttgt tccaatctta cagccatcca cgcttcttgg gccttgcgtt tcagagtctg
    10141 atcctcggca ctggtggaga agaaggaaag tttgatcaat tgctgtgtca aagtacttcc
    10201 gccttgcagt ccgccagagg taaagttaga gagggcagaa ccgatgatcc gaatagggtc
    10261 aaccccgatg tgtttataaa aacggcgatc ttctaccgac acgatcgcat cttctaatgt
    10321 tttcggtaat tcattcgcag aaattttttc acgcttttct gcgcctaaat cttcgaatag
    10381 ttcgccatct tgtgtatata gtttggaaga tacggtagcg tctaattttt tatccgtcaa
    10441 ttcgggcgca tcttttgcgt aataccagaa aagtcctaca cctgctaaaa agaggataca
    10501 gaaaaatgag agtattccta aaaggatctt gataaataat ccttttttat gtgttccaga
    10561 ggactttcca gaacctttgc cagaattctt cttcacactc tttttaggag taggctgttt
    10621 ctgtcgtcta gagcttctag tttgttcatt tgccattctt gctttctcct tcaatagata
    10681 atgttgataa atagaagtca accgcgtcca aataaggaat ccgcggagaa ataccagttt
    10741 tgatctctat tcctagttct tcgatcaaag ataaaggtag tgatttgcgt cctttttcct
    10801 tttgggcgtc ccagtaagca atcaaatgaa cactattcaa ataaaaacag cgcttcaaag
    10861 acgaaaacca taacaagaca aagcagaccc cattttgttc gaggcatgct ttcatatgtt
    10921 cgatttgatg ggcatggaag tttttaaagg gaaaagatgt tttgttcttg gtttcttttg
    10981 cttcaaagtc caagtaaaag cctcgataga cgccattata gtctgtagtt gaggcttggg
    11041 taaagtaggc ttccttgatc acagcagcac ttctcttagg atagtctact ttcacgattt
    11101 ggataggggt aggcttttta tggataaccg ccaacccgcg ggaaagataa tatttattac
    11161 tttcattgat tgcttcttca aagcgcatcc cccgattgcc gaattcagtg atctgatttc
    11221 ttttttcttg aattttttcg acgggagagg cattcttata ataaggttgg ccatttggat
    11281 agcgcaatac catctttaat cacactccta ggccacatta tagcaaaaaa gcggatagaa
    11341 agaaaaggaa tctgagatgg ataagtcaaa agtaatctat gtgtctgggt atcgaagttt
    11401 cgaactcggt gtttttaaag aaaatgatcc taaaatccaa gttgttaaaa atgttttaaa
    11461 aaaagaactc catcagttag cagaagaagg gctggaatgg gtacttgtca gtgggaattt
    11521 gggcgtcgaa ctatgggctg tgcaaatagt agcggaattg aaagaagaat atccggaact
    11581 atctcttggt attttgtatc ctttttctga ttttggtaat aactggaatg aagggaacca
    11641 agaaaaaaaa caattagcag aacaattagc agattatgtc gaagctgtta gccatcaacc
    11701 ctaccaatca cctagtcagc taaagaatca cactcgtttt atacttgagc atacagtggg
    11761 ctgtctgctg atctatgatg aagaattccc tggaaaaacg aagtattttt ttgaagatgc
    11821 acagaattac caagcagatc atccctacga aattcggctg atcagtatgg acgatcttca
    11881 aaattttagt gaataatcga tattgtgttt gatttatcaa cttttttcct atacaatgaa
    11941 aaaggtagaa atgttattac gtaaatgaaa actagaggtg taaatatggc gaatttggtt
    12001 tatagtccta aagacatttt acaacaagaa ttcaaaacaa aaatgcgcgg ctatgatccc
    12061 gttgaagttg atgaattctt agacaatatc atcaaagatt atgaagctta cagcaaagaa
    12121 ctccttgctt tacaagaaga aaacgatcga ttgagtgcaa aagtagctca attatctaaa
    12181 actcaaggag cagctcaaac acgcgtgcaa caaacagagg caccaaagag cgcagcagtg
    12241 accaattttg atatcttaaa acgcttgtca aatcttgaac gtgaagtatt tggtaaaaag
    12301 cttgatcaac aagcaagtgc agtcaaacca gcacaaccta atccaaataa ctacacaaat
    12361 gcagacacaa gcttagatga taatgaaaaa acacgtcaat tttaattttt gattaatgaa
    12421 tttgcggttt tcgggtaatc gcggtctgac ttgatcaggc tgaggaaagt ccatgctcgc
    12481 acaggctgtg atgcctgtag tgttcgtgct tagcgaaacc ataagctaag gtactctttg
    12541 gagtaacggc aggaaaacaa gctaaggctt cggctatgct taagtatcct tgaaagtgcc
    12601 acagtgacga agcggctggg gaaacccatc cggtggaacg cggtaaaccc ctcgagcgag
    12661 caacccaaac aatggtaggg gcgctttttg tctggaagaa gaacaggaca aaaaggcagt
    12721 ataactgcag atagatggtt accgctttgt tttttctccc tggaaaagca aagtacagaa
    12781 catggcttat agaaaaccgc aaacatcagg gaattaggtc gtgacaaaag taatgcgtta
    12841 taagaaacaa gaaaaacatt tgaaagctat ttttcaatac ttcagatgaa tcgttcttat
    12901 tacaaaacaa acttttgtat caccggttat tcggaatttg agtaaaagtg tggggtagga
    12961 ctgatgtccc acctcctttt tttataaata aggagattgt gtatggaaaa aaacaggaag
    13021 ttcaacctcg tcgcaactgc ggcaagcggg ctagaagcac tggttggtaa agaattaaga
    13081 gatcttggaa tcgaatgcca agttgagaac ggacgtgcac gttttgaagg aacaatggaa
    13141 acgatcgcta cagctaattt atggttacgt acagcagatc gagtaaaaat cgtggttggg
    13201 gaattcgatg cgtatacgtt tgatgaattg tttgaaaacg taaaagcatt gccttgggaa
    13261 gattttcttc cactagatgc agcttttcct gtagctggaa aatcgatcaa gtcgaaacta
    13321 tatagcacac ctgattgcca agcgatcaca aaaaaagcaa tcgttgaacg tctccgtacc
    13381 tattatcacc gtccagcttc tgtaccactg acggaaactg gcgcacattt tcagttggaa
    13441 gtagctttgc tgaaagacca tgtgatggta actctggata cgacaggacc aagtttattc
    13501 aaacgtggtt atcgcttaga aaaaggtgga gcaccattga aagaaaacat ggcagcagca
    13561 cttattatgt tgacaaattg gcgaaaagac cgtccatttt atgatcctgt atgtggttca
    13621 ggtacgcttt gtattgaagc agccttaata ggccataata tcgcaccagg attcaatcgt
    13681 gagtttgctt gcgaatcttg ggattggttc agtcaagaaa tcatggaaaa tgttcgatca
    13741 gcagccgaag aaaaagcaga ttatgatatt gaattagata ttactggttc agatatcaat
    13801 ggacggatga ttgaaattgc caaagccaat gcagaagaaa tcggattagg ctcttcgatt
    13861 acctttaagc aacaagcagt caaagacttc aaaacggata aagaatacgg ggtcatcgtg
    13921 gctaatccgc cttacggaga acgtctagga gaagaagaaa ctgttcggaa attatataaa
    13981 gaaatgggcg atgtattccg tccattaaaa acatggagta aatatatctt gactagcgac
    14041 ttagcgtttg aggaatttta cggacaaaaa gcaaccaaaa aaagaaaatt gtacaatggt
    14101 gcattgcgta cggatctatt ccagtattgg ggaactcgtc cgcctagaaa aaaagagaaa
    14161 gacgaataat cttactaatt gattcattca attggatgtt tggaggaata gggaatgaaa
    14221 gaaaaagagt ttttacaaga agtaaaagaa atcaatcttc tacaacaagc tttaggaatc
    14281 ctcgattggg atacacaaac aggtatgcct gaaaaagcta gtgcataccg aagtgaagta
    14341 gacagctatc tttacagcat ttatttttca aaaaaaatcg gtccaaaaat caaagaagcc
    14401 attcgctatt tttctgaaca tccggaagaa ttgtcggaag ttggaactgc cgtttttgcc
    14461 aaagtaaaag aagagtatga tttggaacat cgcgtaccgg aagatcttat gagaaaatat
    14521 acagctgccg tttcttcagc acacggacaa tggcaaaaag ctagagaaac acaacaattt
    14581 tctgattttc aacacgcatt gaatcaaaat attgaattaa caaaacagtt gataccttac
    14641 tggagaaaag atgaaaagac gaattatgat gttttgctga atcaatacga acccgggatg
    14701 actgtcgaaa tccttgataa ggtatttgat cagcttaaag aaggaattct ttcgatccgc
    14761 cgtacgctcc aagaaaaagg gactgaacct gaaacagact ttttaaatcg gagaatgacg
    14821 aaaaaacaac agaagagatt tgtagtgaaa gtaatcgaac aacttggtta tgattttagt
    14881 cgtggtcgtt tagatgacac tgtccaccca tttatgactg gaattaatcc gaatgatgca
    14941 cgaatcacga cacgatggaa cgaaacagat ttcaaaatgg ctgtttttgg agtgatccac
    15001 gaggctggtc atggtatgta tgaacaagac atcgatgata aatatgcgta tacaccggtt
    15061 tatgaaggta catcaatggg tatccatgaa tcacaatcgt tgtttaacga gatcattatt
    15121 ggaagtagtc gcagcttctg gaaaaagcaa tatcctttct ttcaagaatg tgcagaagga
    15181 acattcgatg atattccttt tgacaaattt tatcgttcat tgaaccatac aaaatcaagt
    15241 ttggttcgta tcgaggcaga cagtttgact taccctctcc atatcatcat tcgttatgaa
    15301 atcgaaaaaa tgattttcaa tgaaggggta gatgttgctg aactacctga aatatggaat
    15361 caaaaatatg aagagtatct gggaatacga ccaacaaatg atgtggaagg catcctacaa
    15421 gacgttcatt ggtcaggtgc aagctttggt tattttccct cttatgcatt agggtacatg
    15481 tatgcggcac aattacttca ttctttgcag caagaaatag atgtggaaaa tgttctagcg
    15541 tcagacgatt attctccaat caaaaactgg atgtcagagc atatccatca atatggtgca
    15601 tctagaaaac caaatcaatt aatcatggat gcaacacatg aaccgcttaa tccgcaatac
    15661 ctgatcgact atatgaaaga cctgtatttt aatgtatatc aggtaaaata aaatacaatt
    15721 tacaaagata gctgaaattc atctaatcta tagatgtttt cagctattta cttaagaaag
    15781 aagtggaacg aatggataaa ttacaagtga tcgctgatta tagtatggac caattagcgc
    15841 atgatcaaac aggacatggg agcgatcata ccaaaagagt tgtcaagtta gctgaaaaaa
    15901 ttttggagac ggaaccgcag gcagaccgct ttgtgacgct tgcagcagct tacctacatg
    15961 atacgattga tgataaagtc gttaaaaatg agaacgaggc aaagcaacag cttcgtgtat
    16021 tccttcgctc tttgccaata gtagaggaac agatctcgat gattttttcc attatcgaaa
    16081 atatgtcctt ttctaaaaat ctctctgaag cggccgaact atctctggaa ggtaagatcg
    16141 ttcaagacgc tgatcgtatc gaagcattag gagcaatcgg cattttgcgt actgcttatt
    16201 tcggcggtgg acatgggcac ccgatttttg atcctgaact gtatccgcaa acattcaagg
    16261 acaaagaaaa ttaccgaaaa ggcacgacag tgatcaatca tttttatgaa aaactgttct
    16321 tgctgtctga taaaatgaat actgattacg gatatgaaga agccaaacga agagaaacct
    16381 tcatgcgtga attcttagaa gaattttttc tggaatggtc tattgacgat gaaaatttta
    16441 ctccagacaa ttggatcaaa aataacaaaa aatgatggag gttgtgacag aagtggtcag
    16501 ctccaagata tttcggatca ttttccgtaa aagttacttc tggaacactg tttaccaaga
    16561 ggtcgtgagc ctgccgttca gctccgagtc acaaggaaca atttcaaaaa atagcttctc
    16621 gtgtttgatg aatattgtaa cttgtgattc ggaattggca ggagaacacc gtttattcgg
    16681 aagaatagga ctgtgacaag acttttgtca cagtcctttt ttcgaattca acaagtaaaa
    16741 cttttaagaa atattcagag agtaaaatta aaaattgctg ataaacgaag atggaaattt
    16801 tatgtatttt tattggtcta tgcaataaat aaattaaata ttcagaatac aattgatttt
    16861 aacgaaaggg cttctatcaa tggttgtgtt gtatttatct gcccaggtag agtaaaatag
    16921 atcattatct aaaaggtaat tgttcatact atataagtca tttgaaagta aaaagatact
    16981 gtaaataaaa aatgtggtta aataattctc ataatcagtg taagcgttat caaaaatata
    17041 gcatacttgc tttgtagatg aatgacacga tatatgtttg gtatgttctg caagacagat
    17101 gaatatggag gtggagacaa tgtcttccaa agaactaaca atgtatttag agaaagaatt
    17161 ggatcaactg aaagaaaagg ggttatacaa tacgatcgac atactagaaa gtgaaaatgg
    17221 tgcttgtatc atcgtagacg gaaaaaagat gatcaatcta gcatccaaca attatcttgg
    17281 atttgccaat cgcgaagaat tgaaaaaagc atgtattgaa gcaaccgaaa cttatggtgt
    17341 aggagcagga gcagtccgaa cgatcaatgg ttcgctgaaa atccatcagc aattagaaga
    17401 aaaaatcgca gaattcaaag ggacagaagc agcaatcgcc tttcaatctg gattcaactg
    17461 caatatgggc gctatctctg cagtaatgac aaaagaagat gctatcttat cggatgaatt
    17521 aaaccatgca tctattattg acggttgccg cttatccgga gcaaaaatca tccgaattaa
    17581 acatcaagat atgaaagacc ttgagaaaaa agcaaaggaa gcgatagaaa gtaagaaata
    17641 taaaaaaatc atgtatatca cagatggtgt tttctccatg gatggagaca ttgcacgatt
    17701 gccggaaatt attccgattg ttgaaaaata tggactgatc acctacgtcg atgatgcgca
    17761 cggctcaggc gtaactggaa aaggtgcagg aaccgtcaag cattttggat taagcgacaa
    17821 aatcgatatg caaatgggca cgttatcgaa agctataggt gttgttgggg gttatgtagc
    17881 tggatcgaaa acactaatcg actggttgaa agctagaagt cggccattcc tattttctac
    17941 ttcgctaact ccaggtgcag ctgcagctgc attggcttcg attactttga tgcaagaaca
    18001 tccggaactt gtcgaaaaag tttgggaaaa tgctaattat tttaaagaag aattgaaaaa
    18061 agtaggctat aacatcggta tgtctgaaac acctattact ccagtcattt tgggagacga
    18121 aaaagtgact cagactttct ctaagaagct gatcgaacat gggatttatg ctaaaccaat
    18181 cgtttatcca actgtgcctc ttgggacagg caggatcaga aatatgccga ctgcggaaca
    18241 tacgagggaa atgcttgatg aagcagtagc agtttatcaa aaaataggaa aagaaatgga
    18301 aattatttaa ctaaatttag ttttttcgtc tatttaaaat tagagaacat gttcacgcat
    18361 atattgttaa tcatctgaag atagtagtaa aaaagatcag ggaggatcat catgaaaaaa
    18421 gtattggtga caggatgttt aggacaaatt ggttcagagt tgatcacacg gctacgtcaa
    18481 gaactaggca gtgaaaatgt cattgcgaca gatatacgca tgcctgaagt cattgagaaa
    18541 aacgatatct ttgagatcct agatgtaatg gattacgaaa aaatgcgcag catagcagaa
    18601 caatatcaag tggacacatt gattcatttg gctgctcttt tgtcagctgt tgcagaagca
    18661 aaaccacaat tagcctggaa tttgaacgtg actgggctgg tcaatgcatt ggaagtcgct
    18721 agagaattcc agctgaaatt ttttacgcca agttcaatcg gcgcgtttgg tccaaatact
    18781 ccaaaagatc ataccccaca agatactctt cagcgtccta caaccatgta tggggtaacg
    18841 aaggtagctg gagaactact atgtgattat tattatgtga aatatggcgt agatactcgt
    18901 ggtgtccgtt tccctggatt gatttcttat gcaaccttgc caggaggcgg tacgacagac
    18961 tacgcagtcg atatttatta ttcagcaatc aaagaaggaa agtacactag tttcattgat
    19021 caaggtacat caatggatat gatgtatatg ccagatgcga tcgatgccat tataaaactc
    19081 atgaatgcag atcctgatcg attggttcat cgaaatgcct tcaatatatc ggcgatgtct
    19141 tttgacccgg aagagatcaa agcatctatt caaaaaatca taccagattt tgaaatggat
    19201 tatgatgtgg atcctgtacg tcagtcaata gcagaatctt ggccaaattc actagatgtt
    19261 tcttgtgcgc aagaggaatg gggcttttcg ccgaaatacg acttagatgt gatgacaaag
    19321 gatatgttgg aaaaactgaa agaaaaattg gtagagacgg tataaaacaa gaaccacatc
    19381 caaaaagttt tttatacttt tgggattggc tatggtatga aaggggacaa gggatggaag
    19441 aatatcaagc cacgccagta aaagaagtaa aaatagaaaa tgaattaaaa cgtacgatgg
    19501 gattttttac tgctttatcg actgttatgg gcacagtgat cggtgcaggg gtatttttta
    19561 aagccgcaag cgtagcagaa gttacaggat ccgccagttt gcatatgttt tcttggtttc
    19621 tcggtggtat gatttctgtc tgtgcaggac taacaggagc tgaactggca gctgctattc
    19681 cagaaacagg agggatgatt aaatatatcg aacggattta cggaaataca gcagcttttt
    19741 tattgggctg ggcgcaagtc gtgatttatt ttccagcaaa tgtagcagcc ttgtcgatca
    19801 ttttcggtac acagttcgtc aatctatttg gtctttccca gtcaatgatt gttcccgtag
    19861 ctgttacagc tgctgtatcg atcctgttga tcaatttttt aggttctaaa gctggagggg
    19921 cttttcagtc gattacgctt gtctgcaagc ttattccttt gtttgtgatc gtcattttcg
    19981 gtttgttccg acaagaagga gtagacttcc aactgtttcc gatccaagca ggagaaaatc
    20041 tttcattttt ctcagccttg ggagcgggct tgctagcaac gatgttcgca tacgacggtt
    20101 ggatacacgt aggaaatatt tcgggagaat tgaaaaagcc ggcgaaagat ttgccaaaag
    20161 caatttcttt aggaatcatc ggaatcatga tcgtttattt attagtaaat gcggtgttct
    20221 taagaaccgc atctatcgac ggagtcgcag gaaacagcaa tgcggcaagt gacgttgcaa
    20281 aaatgatttt tggcggcttc ggtggaagac tggtcacagt tggtatcttg atttctgttt
    20341 atggaaccat caatggctat acccttactg gaatgcgtct cccttatgtt atggcaaaag
    20401 aaaacaacct gccttttagc aagctctttg ctaaacttca tgataaaact aaagttcctg
    20461 tagccgcagg tatcttagag ctagtcatcg ctattggcat gatgatgatc ggtggttttg
    20521 atacactaac ggatatgctg atctttgtca tttggatttt ttatacgatg gtctttgttg
    20581 gtgtcattct tcttcgtaaa aaagaacctg atttgtttcg accatacaaa gttccaatgt
    20641 atccgtttat tccactagta gcaatcattg gagggacttt tatcgtatca agcacgctta
    20701 tcactcagac ctttttagct tcgatgggga tagtattgac acttgctggt atcccgattt
    20761 atctctattt aaaacgtaga tagctaaaac attcgaattt tattagaact gcacttctct
    20821 gccaaacaaa atattttatt taaatgagga gtacgtggaa gaaatgtcaa aagtactcgt
    20881 taattttgat cacgtaaatg tcgttttgtc tacgatgaaa tagcaaagta aaagacagaa
    20941 atcgggctca accgatttct gtcttttact ttgatttgcg taactgaact attcggcgta
    21001 cgccgcgata aatatttaat aaaaccaagc aaccagaaat gaaaatggga agccaaatca
    21061 gatagacagg gtagccaagc actttagaca aagcatgtgg agtcatttta tccatttcaa
    21121 gaagtgcttg ctgccaagtc tgaagattga agactagcat gaaacccgaa ataatcagga
    21181 gaataaatcc taaataatga attgtagttc tttttcttac ataagcgatc gtagcataaa
    21241 ggaacagtaa aaaaataggt acgccaaaaa caaagtacat attcagcatc tcgacacccc
    21301 ctttgtgttt atcttacttt actttgaaag aaatgagaaa taatagcctc gttacttgct
    21361 tgaaacagca aaggactgct aagataagag agatacaaag aatcaaggag gagtcatatg
    21421 gcaagttata tcgaaccgat tcgttttgcg ctgattgttt ttccattttt agccttagcg
    21481 atctcatctg ttttttttat ttatgaatat cgaaagtatg gaacatttct agtaacaagg
    21541 gcagttattt tgtattcttt cgttttttat ttattgtgtg cgtatttttt ggtgatctta
    21601 ccacttccgg caaaggagac ggttgcccaa atgacagggc caaagatgga gctgcattta
    21661 tttgcttcat ggcaacattt cagagacaac acggtattgg tcttgtctga acctagtacc
    21721 tacctgccgg caatgaagca gagtgtattt ttggaaccag cttttaatgt tttattactc
    21781 ataccatttg gcatttattt aagatattat ttccggcttt cttttatgaa aacgatgatt
    21841 gctagtttct gcctttctct attttttgaa ttgacgcagc taagtggact ttattttatc
    21901 tacccaagac cataccgctt atttgatgtg aatgatttag tgacaaatac attgggtgga
    21961 atcattggtt ttgtcgttac gccgttattt acatttatgc ttcctacaag aagacgaatg
    22021 gatgaacttt cttatgaaaa aggaaagact gtctcattga ctcgacgctt ggtcgcttat
    22081 ttgatcgact ggacattttt atctcttatt aatttggtag cggcgatttt ctttaaaatt
    22141 gcgttcgatt caggggcagt tacttctcat atctggtggc ttttattaga agtaggggta
    22201 tactttatga ttgtaccgtt tataaccaat ggatggacac ttggcaaaaa aattgtgcgt
    22261 atccgtttgg tggaagaaga ccgcgaacga atttctttaa aagcattgat gatccgctac
    22321 gggtatctgt actttctttt ttatggttta agctggttgg ggcagttgct tcagcaaatc
    22381 atgaattcga caacgaatac ggtgttgcag atgagtgcag tattcatttc tctcggtgtc
    22441 ttgttactac aaggtatttt tattttaaat atcttgctat catttttgag aaaaaaacgg
    22501 attctgtttt atgaaaaagc tagtcacaca tatactgtta gcacgattgg ccaaacaaag
    22561 gatgatgatt ctcgaaatta gctgagcaaa aacgctttgt ctgtgttata atgccaaagt
    22621 cgtgataatt taaagagaag aggtgcacga atgaataatt ttgatatatt ccgcttcaaa
    22681 ccgtttatca atgaagcgtt aaccgcaaag ggcttcaaac agcccacaga ggtccaggaa
    22741 cgtttgattc ctttaattgc aaaaggaaaa aatatcattg ggcaatctca aactggtaca
    22801 ggaaaaacac atacgttttt gttgcctttg tttgaaaaaa tcaatgcaga aaaagaggaa
    22861 gtacaagttg ttattacagc tccaagtagg gaactagctg cacagatcta tgaagcagct
    22921 acacaaatcg ctaaattttc tacaccggag atccgtgtct cgaattttgt cggcggtaca
    22981 gacaaacagc gtcaattaaa tcgtctgcat caccaacagc cacaggttgt agtaggcacg
    23041 cctggtagaa tattagacat gatgaatgaa caagcgctga agattcacac cgcatttgca
    23101 tttgttgtcg atgaagcgga tatgacattg gatatgggtt ttttaaatga agtagaccaa
    23161 attgcaagtc gattaccaga aaagctgcag tttttagttt tttctgccac tattccagaa
    23221 aaacttcgtc cattcttacg aaaatacatg gaaaatccaa tcattgaaga aatcaaacca
    23281 aaagcagtta tttctgaaga cattgaaaat tggctgattt caacaaaagg taaagatgag
    23341 aatcaaatga tttatcaact gctgacaatt ggtcatcctt atttagcaat cgtatttgcc
    23401 aatacaaaac agcatgtgga tgaaattgcc gattacttga aaaaccaagg cttgaaagta
    23461 gcaaagattc atggagacat caccccaaga gagagaaaac gagtgatgcg acaagtacag
    23521 aatctggagt atcaatatgt tgtagcgact gatttagctg ctagagggat cgatatcgaa
    23581 ggtgtctctc atgttattaa tgcagaaatt ccagaagatc tagacttctt tatccatcgt
    23641 gtcggacgta ctggacgtaa tggcttaaaa ggaacggcca ttacgttata ttcgccaaat
    23701 gatgagcaag ccattgatgc gatagaaaaa ttgggcgtct catttgaacc aaaagaaatc
    23761 aaaaacgggg aaatcgtcaa aacatacgat cgtaatagac gtgccaaacg agaaaaatca
    23821 aaagatgtat tagatcccac gctgattggt cttgtgaaga agaaaaagaa aaagatcaag
    23881 cctggttata agaaaaaaat cgattgggca attgcagaaa aaaataaaaa agaacgcaaa
    23941 atcgaacgca gacagcaaag ccgtactgct cgtaaaaata agaaaaattc acataaataa
    24001 aaaacaagag gttgtgacaa aaggcgtgtc acaatctctt gtttttaagc tagacttttt
    24061 ttcattccga tacaaagtac ttcatcttca ataaaaggtt ctgtgcaagt atgataccct
    24121 tgtttttcat aaaaatctac agccgtcatt tcagctacta cataactagt ctgacaacct
    24181 tctgaaaccg cccgtttttc agcagcttca agcaactgac cgcctaaccc ttttcctcgg
    24241 tattcttttg ctacgcaaaa tcgttccggc ttgaagcaag taggtgtttt cttttcataa
    24301 cgcaaagtag ctactggtct tttttgatca agaaatagat aataggtttg ttgcgggata
    24361 tcttttccgt cgaattctag ttctgcagga atcccttgtt ctaatacaaa aacttgatat
    24421 ctcaaaaagc agctagctgc gcgtatccat tcatccgctc caactatttt tttcatcata
    24481 ttcctccttg tcatgaatag cagagatgta ttgattcctt tattcttatc gtaccaaaaa
    24541 tcgtggagta tgaccaaaag gaaatgaaaa taaaaattga gatattgaca gtgatcaata
    24601 gaatgcgtat actaattaag tacgaaaagg atatgatttg ttttttaaga ctcacagaaa
    24661 attctttctt ggctgaaaaa agaatagtca ctaatcaaat tgctcccttt ttctattttg
    24721 gtagaaatac cgacgcagct cagcgttatc gagtttaaga ggtaatgatt acacatcatt
    24781 gcaatcaagg tggtaccgcg agttttcgtc cttggctgtg atggtgtttt tttgtattta
    24841 atttatagaa agcaggtaat gacatgaaac aattaagtag tgcacaagtt cgacaaatgt
    24901 ttcttgattt cttccaatct aaaggacata cgattgaacc tagtgcgtct ttgatccccg
    24961 tgaacgatcc aaccttattg tggatcaact ctggtgttgc aacattgaaa aaatattttg
    25021 atggttcagt tgtgccagaa aatccaagaa tcacaaatgc ccaaaaatct attcgaacaa
    25081 atgatatcga aaatgtcggt aaaacagcac ggcaccatac gatgtttgag atgctgggta
    25141 atttttcgat tggtgactat ttcaaaaatg aagccatcca ctgggcatgg gaatttttga
    25201 ctgatccaaa atggatggca ttcgatccag aaaaacttta tgtaacagtt tatccaaaag
    25261 atacagaagc aaaacgcatc tggcatgaag aagttggcct gccactagat cacattattg
    25321 atgtagaaga caacttctgg gatatcggtg caggcccatg tggtccggat acggaaattt
    25381 tctatgaccg tggagaagct tataacgatt tagcagaaga cgatcctgaa aactatccag
    25441 gaggagaaaa tgaacgttat ttagaaatct ggaacttagt attttcagaa ttcaaccata
    25501 agcctgataa cacatttgaa ccacttcctc ataaaaacat tgatacgggg atgggattgg
    25561 aacgcatggt ctcaatcgtt caagatgcgt caacgaactt tgagacagac ttgtttatgc
    25621 ctattatcca cgccgtagaa aagctaagca aacaagtaaa atatggtgaa aacccagtaa
    25681 cagatatttc ttacaaagta atcgcagatc atattcgcgc cctttcattt gctattggcg
    25741 acggtgcatt gccatcgaat gaagggagag ggtatgtctt acgccgtttg cttcgtcgtg
    25801 ctgtcatgca tgggaaaaaa ttaggtattg aacaagcatt cttatatcgt ttagtccctg
    25861 ttgttgggga aatcatggta agttattatc ctgaagtact tcaacaaaaa gaatttattg
    25921 aaaaagttat ccgtacagaa gaagaacgtt tcaatgaaac aatcaatgaa ggtttggaaa
    25981 tcttaaatca agtgatccaa gaagtaaaaa acgaaaacgg gacgactcta gcaggaaaag
    26041 acatcttcaa actatacgac acatatggct tcccggtaga attaacggaa gaggtggcag
    26101 aagatgaagg actgaaagtt gatcatgctg gttttgaaaa agaaatggat gcacaacgcg
    26161 aacgtgctcg ctctgcccgc agtactgaaa aatcaatggg ggtacaatct gctttattga
    26221 cagatatcaa agtagaaagt aaattcattg gatacacaga acaagaatca gatagtcaat
    26281 tattggtcat cgttaaaggt gagtctcttt tgaatgaagt cgcagaaggt gaagctcaaa
    26341 tgatctttga tcaaacacct ttctatgcag aaatgggtgg gcaagtagcg gatcaaggat
    26401 ggatcatgaa tgaagtaggc gaaatcgtag cagttgttac agatgttcaa aaagcgccta
    26461 atggtcaatt tatgcacacc gtagaagtaa aagcaccaat gagtgaagga caaacctatc
    26521 atctattgat cgatcaatcg atgcgcaatc ggatcatcaa aaaccataca gcaacccatc
    26581 tactgcacaa ggcattgaaa gaagtacttg gcgagcatgc aaatcaagct ggttctttag
    26641 tcgctcctgg acatttacga ttcgacttta cccactttgg tcaaatcact tcagatgaat
    26701 tagctgaaat ggaaagaatc gtcaacgaaa aaatctggga agctatccca gttgaaacaa
    26761 ttgaaacaga tatcgataca gccaaaaaca tgggtgcaat ggctttgttt ggtgaaaaat
    26821 acggacatga cgttcgagta gtaaatattg gcggctggtc aatcgaactg tgcggaggca
    26881 cacatgtagc aaatacagaa aacatcggaa tcttcaaaat cgtttctgaa tcaggaattg
    26941 gtgcaggcgt gcgacgaatc gaggctgtaa caagtaaaga agcgtacgaa ctacttgaag
    27001 aagaagaaaa acaactaaaa caagtagcaa caattgttaa atcacctcaa ttgaaagaag
    27061 ttgtttcaaa agcggaacaa ttgcaacagc aagtaagaga gctacaaaag gaaaatgaac
    27121 aattagctag caagttagcg aatcaacaag ctggtgatgt attcaaagat ccagaaacga
    27181 tcaatggaaa aacaatcatt gcagcacaag taaaagtgaa agatatgaat cagctgcgtc
    27241 aattagctga ccaatggaaa caaaaaaata tttcagatat tcttgtttta gcaaacgaac
    27301 aagaaggaaa agtcagtctg ctagcggcaa tgacgaaaga atcaaatgaa gctggactaa
    27361 aagctggcga cttgattaaa gcaatcgcac cgaaagtagg cggtggcggt ggtggacgtc
    27421 cagacatggc acaagctggc gggaaaaatc ctgctggtat tccagatgca cttaaagctg
    27481 cgaaagaatg ggttgcgcaa caatagggcg tttacgtttg acagaaaaac aaaagttcaa
    27541 acattgaacg aaacatgatt atgggggcga agacaaacaa gtcttgccct catacttttt
    27601 tcaattggat ggcgcatcta atcaaatggt cggattcgga tggtctttag ttgacgaaag
    27661 tgacagaaat ttttacgatt agtataaata aaaaggggtg aaaaaatgaa tagtagaaat
    27721 aagacctttc gactagtctt acgtgcgatc ttgttagcaa tcatcatcgt acaagcgatg
    27781 gttccttggc tggggtttat tccattagga ttcattagtt taacaattat ccatatcaca
    27841 gtaattattg ctgcagtagt attaggccca aaagatggca tggtgattgg cttgttttgg
    27901 ggaatcgcta caattgttcg tgcctatgcc atgccaacaa cgccatttga tacattagtg
    27961 ttcacgaatc cgattatctc ggtagttccc agagtacttg ttggcttagt agcaggcttg
    28021 gttttccact ggatttatca aaaaaataaa tcaataacga tcagctcagc ctttgcaggg
    28081 gtactgggtt cattggtcaa tacagtctta gtgttaggat tcatgggatt attttataca
    28141 ggtgcaacgg cagatgccta tggtgtagac tctgctcttt tatttaaaac cttagcaggg
    28201 gttgcagcaa tcaatgggat accagaagca ataggtgcgg gaatcataac accattacta
    28261 gcaaaagcgt tatttgcagc aacaccgttg aaaccagaat aatgaccatc ttttaatagt
    28321 aataggacgc acgatagata gaaaaaggaa gagaggagag gaaaacaatc gtcctctcca
    28381 tctttttacc tgagcaacaa gcaaactaga aggggtaaaa aacttggcta agaaacaaga
    28441 agaaaaaaag aaacaaatac cgcaacagcg gacaaaaaaa ggagtactag cgggtatctt
    28501 aggattggtt acgaatctga tcttgtttgt ctctaagcta atgatcgggc ttttttcagg
    28561 aagtgtctcc attatggctg atgcagtgaa cagtctatca gatactgctt catccatatt
    28621 gactttgatc ggatttaaaa ttgctgcaaa gccagcagat caggaacatc cttatggaca
    28681 cgaacgcttc gaatatatca gtgggttatt tgtttcgatc atcattactt atgtaggatt
    28741 tcaattttta gagtcttctg ctgaaaaaat catccatcca gaaaatgtct ctttaacacc
    28801 catcgtcttt tttgtcctaa tcatttccat ctttttgaaa tttgcacaag gcaaaatgta
    28861 tcaggttatt gcgcaatcca ttgattctga aacattgaga gctacagcaa cagatagtta
    28921 taatgatgtc tttactacac tggctgtatt gctttcagca gcaatcgaat gggtaactgg
    28981 atggcgtatt gatggatata taggttttgc tttagcatta tatatccttt atagtggatt
    29041 gacgatgctt cgaaattttg tatacgaact attaggaagt cgcccaacgc cagcagagat
    29101 tgaagaaatg gaagagcatt taaatgcttg cgatgatata cttggctatc atgatttatt
    29161 ggtgcataac tatggaccca ataaacgctt tgcatcagtc cacatcgaag tagatgaatc
    29221 aatgaatttg aatgtggctc ataagattat tgatctgata gaaaaagact ttaaaaagtc
    29281 tttggatgtg gaacttgtct gtcacttgga tccaatgcct gttgataacg aaaaatacta
    29341 taatatcttg cggcagttaa agcaaattct tcttgaaaca ggtacagggc ttaatattca
    29401 tgattttaga gtaattcatc aaggaaaaac gctgcaattt gatgtagtgg tccctgagaa
    29461 attccagttt gttgatacag aactagaaag aatcttacgt agtcaaatag aagaaaaaat
    29521 cggagattac caattggaaa ttacgtttga tcacaattat cttttatatt aaatgagaaa
    29581 ctataaaaag actccttaaa gtttttaatt taaagagtct ttttttcttt taattgttta
    29641 tggagagcct gacgtgcaag attatctgct cctttatttt tgttttcggg caaccattgc
    29701 aggatcaata aagaaaattc ggatagaagt tcttggattc tttgaaggta gggaaggaag
    29761 agctcgttct tcgttgcgtt tttgtcgacc gtagaaacta gtgttttgct gtctgaaaaa
    29821 cagaaaatcg tttcattctg taagtcgttt ttctttagaa attctaaagt ttttaaaaag
    29881 acttcgaact ctgcttgatg gtttgtccct atagaaagcg ggatagatag ctgttcttgt
    29941 ttaccttgat agacaatcaa gattccacct ccactaggac cagggttgcc ttttgtagag
    30001 gcatcaatgt atacttttaa cataaaaaga gattctcctt tttgttcaaa ctaatgttat
    30061 ggtaagatta tatcattact gaatcggtga gaggttagag aatgaaaaaa aatattcgat
    30121 ggcagcctga actggcacat acgatcatct attggtcatt gaccttgatc attctgtttt
    30181 ttagtctgat tctttcttta gaaaacacag gtccttattg gaaaagcaat tttgttttag
    30241 gtatttttct tgtttttcta tttctaggtt tgcggcgttc ttttatttta aaatcaaact
    30301 ctgtgattat acgttatgct gctttttgga aagaccgaga aatatctgtc ggacagattc
    30361 gatctttgac gccaactaat actggggtgc aaatgatgtt gcaagatgag aaagccccat
    30421 ctgttttcat gatgcgaaaa aaagatcaag tagtcctttt tgatcatttt gaaaaaaacg
    30481 gacccaaatc agaatcaatt ccttatcctt atacgaaaaa aagaaaaagc taattttaaa
    30541 aatcagaacc taaaaaaacg aatttttaca ggaaaagaaa agagtgagac gtacggttca
    30601 tcccgtttag cgtctcactc tatttgttta tctattcatg catactagta tcggtagtct
    30661 gaacgttgcc tttcttgaca tgggcagctt gtgttcctcg attcccttcg atgatctcaa
    30721 attcgaccac ttgattttct tccaaagttt tgaatccctc ttcttcaatc gcagtaaaat
    30781 gtacaaaaat ttcttctgtg tcatcataac tgataaagcc gtatcctttt ttgttgtcaa
    30841 accattttac aattcctgtg gtcaaagcac tcacgacctt tctaaagtct gatcatagcc
    30901 ttcattataa agagtattta taagtacgtc aaataaatta aaaacctgta attttatgtt
    30961 ttttttctga ataaaaaaag gtataattgg catatcttaa caaagaagat attttgttga
    31021 ataaagttcg tgaaaagggg taatcaaatg aagaatgatc tgcaaatttc acaagaaaca
    31081 gagctagaac cgattgttga agtggctagt aaattagatc ttaaggaaga cgatttggaa
    31141 ctgtatggga aatataaggc gaaaatcaat ttttctggaa tcaatcgatc aaaggataac
    31201 gctgaggggc atttgatctt agttacttcc atcaatccaa caccagctgg cgaaggaaag
    31261 tcaacgatca ctgtagggtt aggagacgcg ctcaataaaa tcaataaaaa atctgttatt
    31321 gcattgcgag aaccctcact tggtcctgtg atgggaatca aaggtggtgc tgcaggcgga
    31381 ggatatgcac aggtattgcc tatggaagat atcaacttgc attttactgg tgacatgcat
    31441 gctatcacaa cagctaataa tgcattatct gctcttttag ataatcatat tcatcaagga
    31501 aatgaattga acatcgatgc ccgccgagtg atttggaaac gggtagtcga tctcaatgat
    31561 cgtgaactgc gtaaagtaat tgttggcttg ggtgggccaa tccaaggtgt tccaagagaa
    31621 gatgggtttg atatcacagt tgccagcgaa atcatggcta tcttgtgtct tgcggcagac
    31681 cttgaagatt taaaggctcg tcttgcaagg atcgttgtcg gctatacatt tgaccgtcag
    31741 cctgtgactg ctggagactt gaaagcagaa ggcgctctgg cattactgct gaaagatgca
    31801 atcaagccga atcttgtaca aacaatttac ggaacaccag cttttgtcca cgggggaccg
    31861 tttgccaata tcgctcatgg ctgcaatagt attttagcta ccaagacagc attgagatta
    31921 gcagattacg ttgtcacaga ggcaggattt ggtgcagatc ttggcggaga gaaattttta
    31981 gatatcaaag tacctaactt gaaaaaagcg ccggatgctg tggtgattgt cgccactgtc
    32041 cgtgcattga aaatgcatgg cggaatgaaa aaagatgaac ttaaaaatga gaatcttgat
    32101 gctttgaaag taggttttgc aaatttaaag cgccatatac gtaatatgga acaatatcag
    32161 ctacctgtga tcgtcgcaat caatgaattc gtaacagata cagacaatga actcacactg
    32221 ttggagcact tgtgtgaaaa tcaggggatt cttgccaaga gagcgagtgt atgggcagat
    32281 ggagcagaag gcggcgttga tttagcaaaa gctgtagtac gattgattga tagaaaagaa
    32341 gctaattaca agccattata ccgcctagaa gaaacgatac aagaaaagac agaaacaatc
    32401 gtcaagaaaa tttacggagg aaatggtgtt gttttcagca aaaaagcgca aaaacaaata
    32461 gaagagttta cgaagaatgg atgggacaaa ctgccgattt gtatggcaaa aacacagtat
    32521 tcattttctg atgatccttc actgctaggt gcaccggaag atttcaccat cacgatccgc
    32581 gaaatcatcc caaaattagg ggcaggattt ttagtagcct tgactggaga tgttatgacg
    32641 atgcctggtt tgcctaaaaa acctgctgca ttgaatatgg acgtgacaaa tgacggagaa
    32701 gtgttgggat tgttttaagc ttgattataa tctaccatcg aaggagctgt attttgacta
    32761 cagcttcttt ttttagacga tagggaatta attttgttta cactaggatc attctttttc
    32821 tttattgtat tttgagctat actaaagcat aataggaggg tatgatgaaa acgacattta
    32881 taagaaaaac cggtttttac ggtatgggta gctcgattca gctaagaatc aacaatgaaa
    32941 aacgcagtgt tctcagtcat gaacaaaaga taacgcttga tctggaagtg ccctttacga
    33001 tgcaggtcac cttttattgg ctaaaaagcc ccgtttatac agtgaaagaa gcaaagcctg
    33061 cttatgtgat cacaatgaat tacttgattc ttcaaatcta tccgttttta tttctagcaa
    33121 ctgggttggg ggccgttttc ctacaaaatt tatttttcag tttttttgca gttattgtgt
    33181 tgattgcgtt ctttttctat ataaaaaacc gggcatattt gatcaaggag gaaaattatg
    33241 gcaaactctg atcttttttt aatcagtttc aatcgaatcg aaaaatggat gagagacgag
    33301 atgggaaatg cacgaaatat gggatttacc gaacttgtca gacgcctagc tcaaagaaaa
    33361 caattaatga tcagaaaata cgaagatgat cttttacagc ttgctcagtt aaggaatgcg
    33421 atcgtccatg atcggatcgc agtcgatttt gtcattgcag agccaaatga ttggacagtc
    33481 aagcgaatcc agctgatcga acaagaactg attcagcctg aaaccgtgct gcctcgattt
    33541 gcaaaacgtg taacaggttt tgaacaagat cttccgttac ttgaactgtt aaagatagta
    33601 gcagaaaaaa ggtattcaca atttccgtta tacaataaag gaaaatttgt agcattgatt
    33661 actttacgta atatcgggtt ttggctagca aaagaaagcc aaaaaggtcc ggttgattta
    33721 acaaataaac gagcgttgga tttgatcatc caaaacggaa agtacacaaa ttatcatttt
    33781 gttccagcta aaacacatat ttacgaggtt gaagcgatgt ttcgtgagca agggactttg
    33841 gaagccgtac ttatcactaa agatgggaat cccgatggta atctgctcgg gatcgtacga
    33901 cctcgtgata tttataaaca aatagaaaag gattgatttt ttgttaatta tttattggat
    33961 attcagcgct ttgctagtag gagtggatca gttggtcaaa tggtggatca ctgataatct
    34021 agcgcttgga gagacgaaaa atcttatacc aggtattttg tcactgaatc atattcgaaa
    34081 cactggagcc gcatggagta tgctagaagg gaagatgtgg ttctttacta tagtgacact
    34141 cattgcagtc gttgtgattc tcacattgat gattaaaaat cgtgaaaaag gtaatcgctg
    34201 gttcatgatt ggattgagtt tgattctagc tggtgcgatt ggcaatttta tcgaccgtat
    34261 tcgtcttggc tatgtagtag atatgttcca gacagatttt atgagctttc cgatttttaa
    34321 cgtagcagat gtgacacttg tatgtggagt gatttgtatg ttaatttata ttattttaga
    34381 tgaaaaggat caaagaaaaa aatgacagaa cttaaaacga cgattcacca tgaatctggt
    34441 aggatcgata aagtattgac taatttattc accgattaca gtcgttcgca aattcagctg
    34501 tggctgaaaa atggagcggt ttctgtcaat ggacagccag tcaaagcaaa ttacaaagta
    34561 aaacaaggag acgagattac tgtcttgatc cccgaaccag aaacattaga tgtggtccca
    34621 gaaaatatcc cgttgacgat cgtttacgaa gatgaagatg tagcggtgat aaacaaacct
    34681 caaggaatgg tcgttcatcc ttcagcaggt catacgcatg gcacaatggt caatgcgctg
    34741 atgtaccata tcaaggatct ttctacgatc aacggcgtta ttcgtccagg tattgttcac
    34801 cgaatcgaca aagatacttc cggactgcta atggttgcca aaaatgacca ggcacacgaa
    34861 tcattagctc agcaattaaa agataagacc tctttgcgca aatatgttgc tttagtccat
    34921 ggagagattc ctcacgaaaa aggaaccatc aatgcgccaa tcggccgttc gaaggaaaac
    34981 cggaaaatgc aggcgataag agaagatggg aagtcggctg tcacacattt tactgtattg
    35041 gaacgtttcg aaggatttac tctggttgaa ttaaaactag aaaccggaag aacacaccag
    35101 atccgtgtgc atatgaagta tatcggttat ccgttagctg gagatccagt atacggaccg
    35161 aagaaaactt tgaaaggcaa tggtcagttt ctccatgcgc aaatgttagg ttttacgcac
    35221 ccgactacaa acgaaaagat gatctttgaa gcacctcttc cagaagtttt tgaaaaaact
    35281 ttagaaaaat taagaaaaaa cattgcattt taatttagat aacggtatag taattaacaa
    35341 cgaaagcata aaacttaata gaattccttt aagtttagtc ccgtgaggct aagaagggtg
    35401 ggagcaagga tacgtatggt gcgccataag tagattgctc attgacatag tatagctatc
    35461 agtgtatcag tatacgctca gtctaaacat tcctcctgtc ctcttagggc aggagttttt
    35521 ttatgcaaaa aagtaggggg aatgaaaatg caagcaaaag aagtagtaga tcaggtaacc
    35581 atgaagcgag cattgactcg aattacttat gagattatcg agagaaacca tagtatccaa
    35641 gatatcgtat tggtaggtat caaaacacga ggaatctaca ttgcatcaag aattgccgaa
    35701 cgcctgaagc agctagaaga tatcgatatt cctgtaggag aactggatat cactttatac
    35761 cgagatgaca aaaaagaaaa tccagaagaa ccggaacttc attcttctga catcccagtt
    35821 tccttagaag gaaaagaagt tattttgatt gatgatgttt tgtatacagg acgaacgatt
    35881 cgagcagcaa tggacgcagt aatggatttt ggcagaccaa gaaaaatttc attagcagtc
    35941 ctggtcgatc gtgggcatcg agaattgcca atccgtgcag attatgttgg gaagaacatt
    36001 ccaacatctc gggcggaaga gattttagta gagatgcaag agcttgatgg acaagatcgc
    36061 atcatgattt tgaaagagga agattagagg agagagaaac atgtcggaga aaaaaggatt
    36121 tcataatgat gacgtggtgt tagatatcca cgatcgccca accccaggac attgggtagg
    36181 attaagttta caacacttgt tcacgatgtt cggagcaacc gtgttagtcc ccatcttagt
    36241 aggtatcgat ccaggtattg cgctagtcag ttcaggacta ggtacattgg tgtacttatt
    36301 tactactaaa ggaaagatac ctgcgtactt aggaagcagc ttcgctttca ttgcagcgat
    36361 gcagcttttg atgaaaacag atggttatcc agcgattgca caaggagcag tcacaactgg
    36421 attagtctat ttgattgttg cgctgatcat caaaaagatc ggttcagcat ggctggataa
    36481 aatcttgcct ccaatcgtcg tcggtccagt catcatggtt atcgggttgg gacttgcggc
    36541 aaatgcagca acaaatgcta tgtataatca gggcacgtat gatttcaagt atattgctgt
    36601 agcgctgatc acattaggat taacaatttt cttcaacatg tggctaagag gttttctcgg
    36661 attgattccg attttactag gtattattag cggatacatc gtagccttgc tgtttggaat
    36721 cgtggatact aagccaatcg tagacgctgc ctggtttgct gttcctaact ttgagattcc
    36781 ctttgtacaa tatgaaccaa agctatatct tggagcaatc acaacaatgg cacccatcgc
    36841 ttttgtgacg atgacagagc atatcggcca tcttatggta ttgaataaac taactaaacg
    36901 taatttcttt gaagaaccgg gtcttcataa gacgttgaca ggtgatggat tagcacaaat
    36961 cgtggcagcc tttgtcggag gtcctcctgt aacaagttac ggtgaaaaca ttggtgttct
    37021 ggctatcaca cgtgtccata gcgtgtttgt gatcggtgga gctgcagtat ttgcagtcat
    37081 tttaggattc gtaggaaaat taagcgcttt gattttaagt atccctggac cagtcatctc
    37141 aggaatcagt tttatcttat ttggagtaat tgccgcaagt ggaatgaaga ttttaatcga
    37201 gaacaaaatc gattttgata aaaagaaaaa tctgttgatt gcttcagtta tcttagtagt
    37261 aggaattggc ggactcgtct ttgaagtagg cacatttaca ttgtcggcaa tggcattagc
    37321 aactgtactt ggcatcattt taaatcttat cttaccagaa acttcacgaa gcgaagaaaa
    37381 ctaaggagga aacattatga tcatcaaatc agaacgtatc agcttaaagc atttattgac
    37441 cgttgaggca cttactgatc aagaagttat gggcttgatc cgcagagggc aggaattcaa
    37501 gcaaggtgct aattggactc cgcaaaagcg ccagtacttt gctacaaacc tctttttcga
    37561 aaatagtact cgcacacata aaagcttcga tgtagcggag aaaaaactag ggctagaagt
    37621 gattgaattt gaagcaagca caagctctgt ccaaaaaggg gaaacactat acgatacagt
    37681 actgacgatg tcagcattag gcgtagacgt agcggttatc cgtcatggtg atgagaacta
    37741 ttatgacgaa ctgatacaaa gtaaaacaat ccagtgttcg attatcaatg gaggagacgg
    37801 aagcggacag catccaaccc aatgcttgct tgatttgatg accatttatg aagaatttgg
    37861 tcattttgaa ggtctgaaag ttgccatcgt aggtgatatc actcactctc gcgtagctaa
    37921 atccaatatg cagatgttaa aacgtctagg cgcacaagta ttcttctcag gtcctagaga
    37981 atggtacgac gatgaattcg aagtgtacgg acattatatg ccattggatg aactactaga
    38041 tcaagttgat gtcatgatga tgcttcgggt gcaacacgaa cgccatgatg gaaaagaaag
    38101 tttctcaaaa gaaggctacc atcaggaaca tgggttaaca gttgagcgtg cgaagaagtt
    38161 gcaaaaacat gcaatcatta tgcacccagc accagttaac cgtgatgtag aattagctga
    38221 ttcattagtg gaaggattac agtcgcggat cgttcagcaa atgagcaatg gagtattcgt
    38281 cagaatggca atcttagaag cagtgctaaa tggaaaagcg taattatagg aggatgaaaa
    38341 aatgaaaaca ctcattaaaa acggtcagat caatacacga aaaaatatga cgacaccagc
    38401 agaaatttgg atagaagatg ggcgaatcaa agcaatcggt acaggattct cagaagccga
    38461 atttgatgaa gtgtttgatg caaagggaca attgatcaca cctggtttag tggacgtcca
    38521 tgttcatttg agagagccag gatttacata caaggagacg atcgaagctg ggactcgctc
    38581 agctgctcgt ggcggtttca ctactgtatg cgcgatgccg aatttaaatc ctgtacctga
    38641 tacggcagaa aagctgagac aagtatatga catcattaga aaagatgcag ttgtgaaggt
    38701 attgcagtat gcgccaatca cagaaaatct gcgaagtgag aaattagttg atcaagaagc
    38761 actgattgaa gaaggtgctt ttgctttcac aaatgacggt gtaggtgtcc aaacagctgg
    38821 gacgatgtat ctggctatga aagaagctgc aaaaaacaat aaagccctag tagcccatac
    38881 agaagatgaa tctttattat ttggcggcgt tatgcacgct ggcaagaagg cagaagaact
    38941 tggtcttccc gggatcttaa gcgtaaccga gtcttctcaa attgctagag atctgttgct
    39001 agctgaagca actggtgttc attaccatgt ttgccatgtt tctactaaag aaagtgtccg
    39061 tgtaatcaga gacgcaaaaa aagcaggaat ccacgtaaca gcagaagttt caccccatca
    39121 tcttattttg atcgacgaag atattcccga agatttcggc ttttggaaaa tgaacccgcc
    39181 gctaagagga cgggaggaca gagaagcttt gatcgaaggc ttgcttgacg gtacgatcga
    39241 ttgcatcgct acagaccatg ccccccatgg attagaagaa aaaagccaaa gtttcatgaa
    39301 atcaccattt ggtatcgtag gaagcgaaac agcgtttcaa ctgatctata ctcattttgt
    39361 agaaactggt cgattcacgt tggaacaagt gatcaattgg ttggcagtta aaccggctga
    39421 gatttttggt ttgaatgcag gaacgctaac ggttggtgcc cccgctgatg tagcagtttt
    39481 tgatattaca caaacttgca cgatcgataa agaagacttt ttatcaaaag gggagaatac
    39541 accttttatc ggctggaaag taaaaggcga aacacaaatg acttttgtca acggaaaact
    39601 tgtgtggcag aagggagaat aaaagtatgg aacgtttgct tatattagaa gatggaacag
    39661 ttttcgaagg aaaggctttt ggtgcagatg ctagtgtctt cggagaaatc gtttttacaa
    39721 caagtatgac tggttatcaa gaaactatca ctgatcaaag ttttaacggt caaatcatta
    39781 catttaccta tcctatggtg ggtaattatg gggtaaatag agatgattat gaatcgattt
    39841 ctccaacatg caaaggagta gtagtaaaag aacatgcacg tgtagcttca aactggcgaa
    39901 accaaatgac gctagattca tttttgaaac aaaaaggaat tcctggaata tcaggtatcg
    39961 atacgcgcgc ccttactcgc aaaatccgtg aagtcggaac gatgaaagct agtatagttg
    40021 atgctggaga ttccttcgaa catgcatttg atcagctgaa agcaaccgtt ctagcaacca
    40081 atcaagtgca gcaagtctca actaacaaac cttatccaag tccaggaaca ggtcggaaag
    40141 tcattgtcat tgacttcggc ttgaaacata gtattttacg agaattgagt aaaagaaact
    40201 gtaacctcac agtacttccc tataacacag atgccgagac gattttagcg ctatcaccag
    40261 acggagtcat gctgacgaat ggacctggtg atccaaaaga tgtgccagaa gcaatcgaaa
    40321 tgatcaaaaa aatccaagga aaagtaccga tttttggtat ttgtctcggt caccagttat
    40381 ttgctttagc caacggtgca gatacctata aaatgaaatt cggacatcga ggattaaatc
    40441 acccggtacg agaagtagcg accggaagaa ttgattttac ttctcaaaat catggctatg
    40501 cagtcgatga agccacgatt gatccagaaa aactcatcgt aactcatgta gaagtaaatg
    40561 acggaacagt agaaggagtc aaacacagag attatcccgc tttcagtgtg cagtttcatc
    40621 cagatgctgc ccctggtccg catgatgcac tacatttatt tgatgaattt atggaaatga
    40681 tggacgcagg aaaggagcag taaacagatg ccgaaacgta cagatatcaa gaaaattatg
    40741 gtgattggat ctggtccgat catcattggt caagccgcag aatttgacta cgcaggaaca
    40801 caagcctgcc ttgccttaaa agaagaagga tatgaagtgg ttttagtaaa ttctaatcct
    40861 gcgacgatca tgacggataa agaagtggca gatcaagtat atatcgaacc gatcacactt
    40921 gaatttgtat caaggattct tcgaaaagag cgtccagatg ctctccttcc aactttaggc
    40981 ggacagacag gcctcaatat ggcaatggag ctggcagcat caggtatttt agaagaattg
    41041 aacatagaac ttctaggaac aaagctttct gcgatcgatc aagcagaaga tcgtgatcta
    41101 ttcaaacagt tgatgaaaga attgaatcag ccaatccccg agtctgaaat cgtcaataca
    41161 gtagaagaag ctgtagattt tgcaaataaa atcggctatc ctgtcatcgt tcgtccagcc
    41221 tttactttag gcggaactgg tggaggtatg tgtgaaaatg aagaagaatt acgtatcgta
    41281 tcagaaaatg gattaagtct ttcccccgct acccaatgtt tgattgaacg aagtattgca
    41341 ggttttaaag aaattgaata tgaagtgatg agagattcgg ctgataatgc aatcgttgtc
    41401 tgcaatatgg aaaattttga cccggtgggt atccatacag gtgattctat cgtatttgcc
    41461 cctagtcaaa cattgtctga ccatgaatat caaatgcttc gtgatgcttc tctttcgatt
    41521 attcgagcac ttaagatcga gggaggctgt aatgtccaac tcgctcttga tccacatagt
    41581 tttaattatt atgtgattga agtcaatccg cgtgtttctc gttcatctgc gttagcaagt
    41641 aaggcgactg gctatccgat cgctaagcta gctgctaaaa tagcgcttgg attaacatta
    41701 gacgaaatga aaaatcctgt tacaggaacg acttttgcag aatttgaacc tgcattagat
    41761 tatgttgtag ctaaaattcc tcgctggccg tttgataagt ttgaaaacgg agaacggctt
    41821 ttaggcacgc agatgaaagc aactggtgaa gtgatggcta tcggtcggaa tatcgaagag
    41881 tctttattga aagctgtgcg ttctttagaa atcggtacac accatatcga acttcctgag
    41941 ttggcagaca tcacggacga tgacctaatc caaaaagtgg ttcgagcaca agatgatcgc
    42001 ctgttttatg tagctgaagc tattagaaga gggtacacca tagaagaatt agcggatttg
    42061 acaaaaatcg atttattctt tttagataaa ctgttgcata tcgtagaaat cgaacagcag
    42121 ttgaaacagc agccgttgga tgacaagata ttgaaaacag cgaaacaaaa cggttttaca
    42181 gacaaaaaag tggcggagct gtgggaaata aacgaagaag ccgttcgcca gatgagaaaa
    42241 gaaaatgatt tgattcctgt atataagatg gttgacactt gtgcagcgga attcgaatca
    42301 aaaacacctt atttttatag ttcctatgaa tatgaaaatg aaagttcacg atcagaaaag
    42361 ccctcaattc tagtactggg atctggtcct atccgtatcg gacaaggggt tgaatttgac
    42421 tatgcgactg tgcattctgt caaagcaatc cagcaggcag gatacgaagc aatcatcatg
    42481 aacagcaatc ctgaaacagt ttcgactgac ttttctattt cggataaatt atattttgag
    42541 ccattgacct tcgaagatgt gatgaatgtg atcgagctag aacagccgat aggagtaatc
    42601 gttcaatttg gtggtcagac agcaatcaac ttagccgaac ctttgactgc tgctggtgta
    42661 aaaatcttag ggacaacgat cgaagacttg gatcgtgcgg aaaatcgtga tttattcgag
    42721 caagcgctga aagagttgga tattcctcaa cctttagggg atactgctac aagtaaagaa
    42781 gaagccgtgg ttatcgcttc gagaattggt tatccagttc ttgttcgtcc aagctatgta
    42841 ttaggcggac gtgcgatgga aatcgttgaa aaccagcgag atttagaaga ttacatggaa
    42901 catgctgtga aagcatctcc agaacatcca gtactagtgg accgttactt gcttggtggt
    42961 gaatgcgaag tggatgcgat ttgtgacggt gaaacagtac tcatcccagg gatcatggag
    43021 catattgagc gtgctggtgt ccattcaggg gattcaatgg ctgtgtatcc acctcaaaca
    43081 ttatctgaat cgatcaagaa aacgatcgaa gattacacga tccgcttagc attaggtttg
    43141 aattgtgtcg ggatgatgaa tatccagttt gtcatccatg aaaatgaggt ttatgtgatc
    43201 gaagtcaatc ctcgagcaag tcggactgtt ccattcttaa gtaaggtgac aggaattcca
    43261 atggcacaaa ttgcgacgaa agctattttg ggagagaaac taaagacact tggttatgaa
    43321 aacggacttt atccagaaac aaaagaagtt catgttaaag cacctgtctt ttcttttacg
    43381 aaattgcaga aagttgatac ttacttaggg cctgaaatga aatcgactgg agaagtaatg
    43441 ggatctgaca gacaattaga caaagctttg tacaaggcat tcgaagcttc tggattacac
    43501 ttgcctgaat atggagccgt attatttacg attgcggacg aaacaaaaga agaagcacta
    43561 gaattagcta aacgttttac agaaattggc tatagtctga taggaacgaa acatacagct
    43621 gcttatttcg aaaaaaatgg tctggtcgtt gaaacagtag cgaaaatttc tgaggaagca
    43681 gcagaaaaaa atgtagtcga cttaataaga gatggtaaaa cacaagtggt tgtcaatacg
    43741 attgataaag atcggggaaa tgcttctaaa gacggtttca tcatccgaag agaagcagtt
    43801 gaacatggta ttccattgtt tacttcttta gacacagcag atgcgatcgt tcgagtgatg
    43861 gaatcccgtg ctttttcaac taaccctatt taacggcgag cacatgttaa gacaaaaaga
    43921 cgaaaaaccg ttgcaggagg ggacagatga aacaagaaat gatggaaatc gttcagcaaa
    43981 aacagctggc accaaagatt tttgaaatga cattgaaagg tgatctagta aaagaaatgc
    44041 agcaaccagg acagttcttg catattctcg tgccgcgtgc agacttgcta ttacgccgac
    44101 cgattagctt gaatcatatc gaccacgaaa caaatacttg ccgtatcatt tatcgggtgg
    44161 aaggtgaggg gactagagtc ttctctactc tttctgccgg agaccattta gatgtcatgg
    44221 gtcctttagg caatggtttt gatttgtcca atcttcatac aggagacgaa gtatttataa
    44281 tcggtggagg aatcggtgtc cctcctctat atgagttatc aaaacagtta gtcgaaaaag
    44341 gaattaagcc aacgcatttt ttaggttttg ccacacatga agtcgtttat tatgaaaaag
    44401 aatttaatcg tctaggagaa acaagaatcg ctactgacga tggaacatac ggtatgcacg
    44461 gaaatgtcgg taatttattg ttaggaacga agacggaacc tacagctgtt tttgcatgtg
    44521 gttcaaacgg gttattgaag acagtggaac agctgttttc ttcgcatcaa aatgtccagt
    44581 tatcattaga atcccgcatg gcatgtggga taggggcttg ctatgcttgc gtttgtcata
    44641 cgccggaaga tgaaacagga acaaaaagtg tgaaagtttg tgacgaagga ccgattttta
    44701 aaataggaga ggtaattata tgaatgagaa tcctttagcg gtcaaacttc ctggcctaga
    44761 attaaaaaat cctatcatgc cggcaagcgg ctgtttcggt tttggcaaag aatacggaaa
    44821 atactatgat ttgaatcagc ttggatcgat catggtcaag gcaacgactc caaatgcccg
    44881 ctttggcaat cccacgccaa gagtggcgga aacaccatct ggcatgttga atgcaattgg
    44941 tttgcaaaat cctggtctag acgttgttat gcataaactt cttccagaat tagaggagta
    45001 caatgagtta cccatcatcg caaatgttgc tggtgcttgt gaagaagact atgtagaagt
    45061 ttgtagcaaa attggcgaag caccaaatgt gaaagcaatc gagctgaaca tttcttgccc
    45121 aaatgtcaaa catggcggaa ttgcttttgg aacagattct gatgttgctt ttcaattaac
    45181 acaagcagta aagaaagttt cttctgttcc tgtctatgtc aaattgtccc caaatgtaac
    45241 agacatcgtt ccaatcgctc aggcaatcga agctggaggg gcagatgggt ttacgatgat
    45301 caatactctt cttggtatgc ggattgacct taagacaaga aaaccgattt tagccaatca
    45361 aacaggtgga ctctccggac cagcaatcaa gcctgtcgct attcgtttga ttcaccaagt
    45421 agcaagtata tccagccttc caatcattgg gatgggcggc gtgcagacag tggatgatgt
    45481 tcttgagatg tttatggctg gagcaagtgc tgtagctata gggaccgcga atttcaccga
    45541 tccgtatatc tgtcctaaac taattaaaga gctccctgtg cgaatgagcg aactaggcat
    45601 cgaatcgctg gaaagattaa tcaaagaagt gagagaggaa cgtgaacgat gaatcaacta
    45661 gaccaacgac caattattgc attagatttt tcaactaggc aagaggtaga agactttctt
    45721 cgatttttcc cgaaagaaga aaaattgttt gtgaaaatcg gaatggaatt gttctaccaa
    45781 gaaggacctg aaatcgtacg ttatctaaaa gaggctggtc ataatgtatt tttagatctg
    45841 aaacttcacg atattccgaa tacggtcgaa aaagcaatgc ggggattggc aaagcttggt
    45901 gttgatctca cctgtgttca tgccgctggc ggtattcgta tgatggaggc ggcaatgcga
    45961 ggattagaag aggggactcc agaaggtgga aaacgtccgc tacttctagc tatcactcag
    46021 cttacttcta caagtgaaga agaaatgcat gccgatcaac taatcgaagt ttctttagaa
    46081 aaaagtgtga tccattatgc tagctgtgcc aaaaaagcag gattagatgg tgtggtttct
    46141 tctgcttggg aagtagaagc gatcaaagaa atggcaggag acgaatttgt ttgcttaact
    46201 ccgggtataa gaccagaagg aacagtcgca ggagatcaga caagagtagt cacaccttct
    46261 caggcaaaaa agatcggctc aacttttatc gttataggtc gaccaatcac tcagtcaact
    46321 aatccttata aagcatatca aacgattcaa acagaatgga gctaacctaa aatgaatgtt
    46381 gaacaaagta tcgcaaaaga cttattagaa attgaagcag tatttttaaa tccaagtaat
    46441 ccttttacat gggcaagtgg aatcaaaagc ccaatttact gtgataaccg aatcacgatg
    46501 agctatccaa aagtccgaaa agagatcgca aaaggattag caagcaaaat caaagaagct
    46561 taccctgaag tacaagtgat tgctggaact gcaacagcag gaatccctca tgccgcatgg
    46621 gtagcggaaa tactggattt gccaatggtc tatattcgaa gtaaggcgaa agatcatggg
    46681 aaaggtaacc aaatcgaagg acgtatattt gaaggtcaga agatggttgt gatcgaggat
    46741 ttgatttcta caggcggaag tgtcttggaa gcagctgaag cagcaaaaag agaaggtgcg
    46801 gatatccttg gtgttgcggc tatctttacg tatgagttgc caaaaggaaa agcaaatttc
    46861 gaaaaagccg aaatccctct tatgacgttg accaattatt ctgtattgat tgaagcagct
    46921 ttagaagatc gttatatcga tgagcaagaa ttaactctgc taaaagaatg gaaaaaagat
    46981 cctgaaaatt ggcagattga agcgcgcttg atgaagatag agaaatccga tttgtgtctc
    47041 cagaatcatc aattggaata gcaaaaatcc aaaaactgat tgcgagaagc atcttttcat
    47101 caggtatgaa aagaaacttc tatcgtgatc agtttttgat ttttttaaaa ttcatttttc
    47161 taagactggt tagcagaaag aagagaataa tctcttctct tttttctatt atatatagat
    47221 tttctctatt ttttggtaag ggagctgttt caaaaatggt aaagagcgga aatgttgcga
    47281 atatcagtta tttaatgata tgatattcgg aaaaagaaat ctcaaaggga ggtaactttt
    47341 atggggttga atgaatttat tttaggattg aacaatttag agacgatcac aagggcacct
    47401 ggatttttta aatatacaga gcatacagtt gcagcacatt cgtatcgtgt agcatctatt
    47461 gcgcaagttc taggagacat tgaagaagcg aatggacaac cgatcgattg gaaactgctc
    47521 tatgaaaaat cattgaatca tgactacaca gagcgtttta ttggtgatat caaaacgccg
    47581 gtgaaatacg caagcaaaga gttaagaggg atgctgcaaa cagttgaaga aaagatgacg
    47641 gacgaattca tcaaccaaga gattccagct gagttccaag aagtttatcg ccgtcggttg
    47701 ttcgaaggaa aagatgaaac cgtagaagga gaactattag ccatttcaga taaggttgac
    47761 cttctttatg aatcttttga tgaaatcatc aaaaataacc cggaaaaagt ttataaggag
    47821 atgttcattg aggcagtgac tacgattaaa agctttgaac atcggccttc tgttcagtat
    47881 ttctttaaag agatatttcc ggaactattg aaccaggaat tttatggaaa agaggcgttt
    47941 ttagaaaccg tctcaacgat tgtaaagaaa gaagaaaaat aaaaatgata tgagatgcag
    48001 ggggaagtat agttgaaaaa gaatatggat gtcgaatggg gctattctgg agacaaaggt
    48061 ccggaaaatt gggcgagttt atgtgattgg tttgcgagag gtgcggaatt tccattgcag
    48121 tcgcctattc atttgaccaa gggggaagaa accaaaatag aaggagacac gctttccttt
    48181 cattatcaaa agcaacggtt tactgacaag gaattcaaaa acacgattca tttagtacct
    48241 tttgaccaac tcagttatgt agagttcaag aaagaacgct attatttgac agatattcat
    48301 ttccacttgc cttctgaaca tatcatcaac ggaaaacagg aagatattga atttcatttt
    48361 gttcatatga acagcaaaaa agagaatcta gttgtgggtg tcatgtacca gctaatggta
    48421 caggaaggtt ggctgtataa tcaagaaaat ggggaaagtt ggaacttaga aaaacatgag
    48481 cattgggtaa atccggatgt gttctttccg gagcaaaaaa gccatttcca ttatattggc
    48541 tcattgacaa ctccgcctac cagcgggcca atccaatggt ttgtaatgga tcatgtagga
    48601 aaactgaacc aagagtttct tctccccttc gatggtcagt atgctcgacc gaacaaccga
    48661 ccaatccagc ccttgaatgg aagagaaatt tattatttcg aggagtttga gcaatgaata
    48721 ttcagcagat gaaatacgtg gtcgctgtag caaacaatgg tagtttcaga gaagcggcaa
    48781 agaaaatgtt tgtctcccag ccaagtcttt cagctggtat caaagaatta gaaaccgagt
    48841 tagggatcac gctgtttact cgaacgaatc gaggggcata tttaacagaa gaagggcaag
    48901 acttcttaaa tcgtgccgag aaaatattgg tccagcttga tacattagaa actcactatc
    48961 taacgagtga aaagagagac aatttttcga tcgctgccca gcattatgat ttcttaggtc
    49021 ctttgaccgc acagctactc gaggaattca aagacgagat caaacaattt cggatcatgg
    49081 agacgacaac agcgagagtg atcgaagaag tcaaagagca gcacagtgag atcggcatta
    49141 tttatatgaa tgagcataat caagctggta tcagaagata tttacaacaa ggagagctaa
    49201 gtggccgcag cattggaaaa ttcaaaacgc atattttttt aaggcaagga catccattag
    49261 ctgaacacgc agagatcacc aaagaagaat tgttatctta tcctcaagtt cgttttacac
    49321 aggatggaaa taatttcccg tatttttatg aagaccttat cgagatccct gatcaagaaa
    49381 cagtgattta tacgagtgat cgaggtactt tgatgaatat cgttcttgaa actgatgctt
    49441 acgcttcagg atctggtatt gtgattggag ggattcgcga gcacttgcgg ttaatccctt
    49501 tggcagaagg cgaactaaat gaattttata tcatttattc ggcgaaacgt ccgctcagtg
    49561 aaatcgctca aagatttatc aaagagctta ctcggttact ggaccagcag agctaagaaa
    49621 agcagatgag aacgctgttt attgaacacc gttgatcaaa aaagaggggt tgtgacagaa
    49681 ttttgtcaca actcctcttt ttgtagcaaa caatttataa ctaattgctg ttgtgggaaa
    49741 gtacgcaaat atgattctag aaacactcat tgattacctg ttttgtttca tagctgtaat
    49801 tttttcttct tctggtgtca cgataatcgt cttttggttt tcataaataa cataaccagg
    49861 cttggcgcca tttggttttc ggatatgctt tacctgtaca agatcaaccg gcacttgggc
    49921 agagtatcgg tattttgaaa aataggcagc taactcggca gcttctgtaa ttgtttcatc
    49981 tgagggcttg tcacttttaa tgatgacgtg cgaaccgggt atattttttg catgaagcca
    50041 atagtcggtt tttttagctg tcttcattgt cagctggtca ttttgaagat tgtttttacc
    50101 aacaagaatg agcgttccgt cagaagaaaa gtattgatca ggctgagatg gtttcttacg
    50161 tttttgtttt tttaagttct tctttttcag gtatccttct gaagtcaatt cttctttaat
    50221 tgcttcgata tccattgggc cggcaatttc cagttgagat aagacagatt caagatattg
    50281 gatttcatct ttggcttctt ggatttgttc gccaatcagc ttaacagcgt tcttcagctt
    50341 ttggtatcga tggaaatatt tctgtgcatt ttgatttggt gtcaatgccg gatctagtgc
    50401 tattttgatt ggccgatctt cttcataata attaggtaaa acaacttctt ttgcgccgcg
    50461 tggtacttgt gtcagaaaag tagtaagtag ttcgccattt ctacggtaat tctcagcgtt
    50521 ttcggattct ttgagtgttt gttcacgttt tttcaattta tttttatttc gttttagttc
    50581 attttcgatt tttcgaatca gttctccgcc ttgttgtttt gcccgatctt tctctgcttt
    50641 ttcgtgataa tagctatcta ataattgact gagtgttgga taagctgtta cagcagatgc
    50701 ctgttcggac aatacctgat aagcaatcgg tgtgaaaaaa tctttatttt ccgtttcgta
    50761 aaaagtagga ataggctgat ggctgatggc tgagaaaaat tcgtcccata caaccatctt
    50821 ttcattagga cgttccagta atcttttggt cagctcttga gcgatatcga aaccgattcc
    50881 ctgaaactga cgttggattg cttttggatc tagttcagtt tgggaaagcc gctgaaagat
    50941 tttttctttc tcggagctaa atgggttcaa gacctcttgt tttggcggag caacatattc
    51001 tacaccagga agcaaagaac gataagtgtt ttgagagctt ccaatgtgtt tgatcgcatc
    51061 cagtattttt ccagtttctc gattaactaa gacgatcgta ctatgacgtc ccattaattc
    51121 aacgatcaat atgatatttt gtagatcacc caactcgttt cgtttagcaa agtggaaatg
    51181 gatcactcga tcattttcga tttgttcgat gtcttctaaa atagcgccat ccaaatattt
    51241 tcgaagcatc atcacgaagt taggcggatt atcaggattc tgataatcga tttgggtaat
    51301 ctgtacacga gcataacttg ggtgcgcaga taataataat cgatggtttt tgccgcgtga
    51361 acgaatcacc agcacgattt cattttcata aggctgatgg attttcgaga tccggccaga
    51421 tagtaacgtt tcacgcaatt cgttgaccat cgcgtgggta aatacaccat caaaggacat
    51481 ggttcattcc tcatttcttt tcataagtat attcattata acgaaaaaaa gaagaaaaac
    51541 caatttggcc gaatagtaaa gcaaaagtct agaaaaatct tggactaatt acataaaaaa
    51601 cagtgtttct atcgttgaaa tccaaatttt taggt
//
LOCUS       DACDXO010000013        51460 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E23605_2017
            SAMN12639989-rid9881463.denovo.013, whole genome shotgun sequence.
ACCESSION   DACDXO010000013 DACDXO010000000
VERSION     DACDXO010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639989
            Sequence Read Archive: SRR10023389
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 51460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741914.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:42:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,972
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 33 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 17 of 73
            Pseudo Genes (multiple problems)  :: 10 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51460
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639989-rid9881463.denovo.013"
                     /isolate="E23605_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            83..1081
                     /locus_tag="IWM35_000901"
     CDS             83..1081
                     /locus_tag="IWM35_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP9918770.1"
                     /translation="MSNAKFNPQAFQTKPPKQQKEKKQKKRFLFQKKKTEPSDRMIQF
                     SQKSAMSVFSGAFFCLFLLMGVVLLLNFGRVDTLTRLAVKKQVNPETLLTEVNQTLSS
                     TEQLKYEGQHFIERLFTYSPEKRNEWEASVNHYLAQGLEASDLGFVTTATNRVAKDVR
                     FIKLEEIDLTQAMYRLFYEVTFTEGEHWKSSQLSFPVSYQNNVLKVLDGVQFLNLSES
                     KNTNSVNYQENRFYSSGQAVTESEEIKLEAFVKRFFELYVKNDEKLALISNEKGLEGG
                     ELINVQLDHAVKNKEGNYFLQGKYRFYFEKGNEFQSNFSMEVKTSGDSYFVTTLNE"
     gene            1105..1389
                     /locus_tag="IWM35_000902"
     CDS             1105..1389
                     /locus_tag="IWM35_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9918771.1"
                     /translation="MKLFDLKSLFDDVSGQVIWLLAIVAIVLMVGAYATQGFARAAFA
                     IIGVFILIMLVLLLSNAVDIGEWLKNIIFIKDSEENVGVTQMIVPWIQLS"
     gene            1470..1868
                     /locus_tag="IWM35_000903"
     CDS             1470..1868
                     /locus_tag="IWM35_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP9918772.1"
                     /translation="MEYNYTKEFKHPHKIYGIKGQKIPWISNGIRMEQLVIGGVFLFI
                     LLILFLIAYVNQISFLQTLFRKSWLIMIFGIGVLVWTLFSMKWDNKHFFEYLIGRTSY
                     YQKRKQRFEHEMYVPFFHEKVTYSLHKWKE"
     gene            1878..4421
                     /locus_tag="IWM35_000904"
     CDS             1878..4421
                     /locus_tag="IWM35_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion protein"
                     /protein_id="HAP9918773.1"
                     /translation="MAFLFLLVVFIIGIWQFKKKKTKLPRLEFPIDAIIRNFVLTKND
                     DVWVGYQLSSQVFPLNDIDFFKNYLQDGEGILGHDEYDYHFINIPRQFNLDTHIEETI
                     RDLVKGECADLGKVYFEQAGKILKDEVQLNEYRTYLFVRFTTEMKVSDPIEYFQLLKH
                     VFKKFIYRITGQRVPISSVLPTYETLEKELYTELSNYKPIERLSAQEIGQIYYYFFHR
                     SEEKLPERQLTPMEICEGVISNEAGYIAIEQLNQTHYSTFLSQTDAPLAMYGSSFIQN
                     LQDSLSFPIDTHIRLRFEHSKSDERKINKMAGRIYEQEKEYETVDGILDSDDVLNFGT
                     ERLMDVKDKLKRKEKRLCYSTVTLVVSASSKEELEERVKDIEFAIESTDFKFYRSVAD
                     QLTLFNQCLIGSTQRFRSYEQVATTGFVADYGLDLKKEVGNKKGFPLGRIVIAKKFKS
                     AKEALALSSKLVWYYPNLTKKSIEGAEHTNGNTLIIGPPGKGKSVLVKYIFLWLMFLG
                     QKILYIDPKNETQKFFLKALEKFGHIPEFVAMYERINFISLSSDESCRGMLDPLLFLP
                     KEEAIQTAKNVLGMLGEVDTNRETASHKKTVIQDAIDVVYTMPGKHSLSQVIEEIRKS
                     DKELAKLILGHSVGLGKILIGNEHSTPIRFDSQINVLGTQGIRIPTQKEIDSGRLNSE
                     QLAGMAIMEVIMKFTYIFSTNKEEDAAIIYDEAKGYEDTAQGSYLIDDSLRKGRANNT
                     DIYLVVQAFKDYDQEDKKELISYKFAFRPKQKEAREKLLSFFDMEVNSANLEMMEQLQ
                     TGTCLFQDHLGRNQPIAIDVLFDSWLLAVSSTENQDEKIQAMLENEKQR"
     gene            4443..6785
                     /locus_tag="IWM35_000905"
     CDS             4443..6785
                     /locus_tag="IWM35_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9918774.1"
                     /translation="MRKKSLKWWTLLLLGGLFFCLQGATVSADQMNDGAGVTETDRSE
                     GENANDKVEVKEVTTDVDQLYKNYKKNSFELMTNDYESMNLKKKLSYNVAGVMKNFVW
                     SGVKMLGEFNASMVEFLFELDIISAIREPIQNLTQKIADNMLSIAGTIGISFSAIMMA
                     IKYAGEARMKQALRVFLMTILIFTGLSVLKDSGTNKNLFDVLFSLDSTVETAFVKVNP
                     VLTPEEETNYETKETEEVTADKKVETAGKLMAARVFYSNVYEPYLLLNYGTTNVDTIR
                     EKKVKYAGKEYDRINLLLDNDLGTKIGEKIHDKVTEYEADTLKNKNIQYYNSWKNTAF
                     ALFYFVVNLIQTVVYFVLSMFRLIIGTMQLLLVPMLPFLLFLGLFLVTMNVFGNYAKV
                     FGILTFLKGMTGFACILFSSFLSYGFQLSSTQDDPWEKILLIIVYLFAPLGIYLFRGF
                     LGSLFTGRMGVRGALAFAGNPFGAEAKLRREAKQRLKERKEARKAQKQKEKENARKRQ
                     ERQRKRGGGSDVRMPQTAKQATRSAFRRESAQQPRHKAPNRMERMAQKARDLHDQAKQ
                     AEEQSQERMQRTAMEKQARKTADLLQRELQGNPSLRERSRKGASRSPYRNQGSAMVVS
                     LANPKQKHKSYGQKHTPRTELNDRKRRQGQTSRRRKEALTPVTKTSSMKTRTVAGQKH
                     AVQKPLRKGNVPRATGSTTPKSVRRPEGVREHMSAVNRVTAQTKAPSTVSKAYSTMKQ
                     TKSPKQAPKTRSEFRATQAKMKRKREKQPIYRREMKQRKR"
     gene            6801..8975
                     /locus_tag="IWM35_000906"
     CDS             6801..8975
                     /locus_tag="IWM35_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 family protein"
                     /protein_id="HAP9918775.1"
                     /translation="MMNAPKRPTHFEQMFSVDRKRKQEKETPKSRFQQLYFIVTLLGV
                     CLYPFFIVGSIPLAIVFMIDKKETRAHIYDFEYESFLKRRSSLFFIFALGFTLLNLFC
                     FIFVLPRGYLSCYLLFPLNLFSHALQFNGQTIFALLLGGTGAGSCLIAYASFIEKRKV
                     VSKKERQTALLNSKEYKERRKNKFQESQKYTAEYQQAYQVAIVIKDVALRNKQLASLA
                     KVILLGTDEYGLPYVMNFSELNQHVIIPATTGSGKTTVIQLLAEHACKFRIPVILVDG
                     KGAGETLQALKEVAEAYNRPIEVFVDGGDLRYNPVKNGNDIAIRDKLTNLAETESVYY
                     STAAKALLQVTIQLLDEFQKSHKIERNLVWLQHYLLPRNVLHLFADRIQLKNPDLFKE
                     KVVVEKKKTTKKKTKKNEAMDKTERDFADVEQLSEERIVEHDRKTVDLETYYLLLKEN
                     ISLMTEIEQTLFKRLFVRYEHKKNPFYLYATSENLQTNINMLLDSELGELFDTSGGKR
                     ELDIQEVVKEGKIVYISLNGLIYSEFIRTLAQMLVGDINFFASEVYKQAGETANRSKV
                     LVIFDEPASYLNDNLIDLVNKGRGAGVHAAYAPQTLADIDRIDPVLRKQLIGNVNTFF
                     IGKTNDPTEIDYWADLMGTEEDIELTEMTTQEAGYSDMGKVDWTGERGTKRDVDRFKF
                     NPNRIRALRTGEFVVYRTAEDVQEPPRVVYIRKPNLEKQYAK"
     gene            8965..9387
                     /locus_tag="IWM35_000907"
     CDS             8965..9387
                     /locus_tag="IWM35_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HAP9918776.1"
                     /translation="MRNKKVIVRILIVSFLLFGCFKLIKTNEESNGKLINSNLEEVLE
                     LQKTNQSFFVYLARNDCKKCQEVESFLERYDKKFSRNVYRIETRKEPRQEELQTFLQR
                     YHVKSVPSFLVIHDRNVKKIERKTFFFEKTLNQFLPNF"
     gene            10268..11179
                     /locus_tag="IWM35_000908"
     CDS             10268..11179
                     /locus_tag="IWM35_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9918777.1"
                     /translation="MKYLLHGYFIDYSTRNSSTLAILCLLHELRVVTRKQLYKLLNFD
                     RSISKQGVTNALVRLYEKQLIDRKKYGTEMYYYLTKEGHQSIGGYYTLPKVPEYNLNH
                     HLQINNYLIKGMALAKDHPHLKRIISERRTVYETKDLNPKTKGRKFFVPDFIFRFRDK
                     ENHEINWSFEIELTLKTRRRYREAIFPKYIRELRTRPESHLIYVTPSGAIQEELHAFK
                     RIFFKRETEKDASQQELFERLHIFSALEFEEGLKALLENDPAVNWRTVEREKIYPNGA
                     VQSRLNLFEESTENEEKPKVKFTPEVL"
     gene            11666..11932
                     /locus_tag="IWM35_000909"
     CDS             11666..11932
                     /locus_tag="IWM35_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001245899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9918778.1"
                     /translation="MTTKNEQLKEQARKELQERGLIIEGSFEGDFETYIGCYARPINK
                     PTALDPMNEQEVKEQEKHAVNGFPQDFTEWYEWEINNGKLENFL"
     gene            12004..12345
                     /locus_tag="IWM35_000910"
     CDS             12004..12345
                     /locus_tag="IWM35_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9918779.1"
                     /translation="MAKNTPYQVGDIFYSSWGYEQTNVTFWQIVKLTEKTAWFQPIQK
                     KTVEHYSSMSGKVVPMKDKFIEYPLAKTKDSIVKKRIDPETYPDMLCGYRSWDTFHRY
                     KNQAVYESSYY"
     gene            12400..12819
                     /gene="ssb"
                     /locus_tag="IWM35_000911"
     CDS             12400..12819
                     /gene="ssb"
                     /locus_tag="IWM35_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAP9918780.1"
                     /translation="MMNSVNLVGRLTKEVDLKYTPKGNATGTFILAVNRNYTNANGER
                     EADYIRCVIWRKAAETLAKYTGKGSLIGINGRLQTRSYQNQQGQTMYVTEVLVTDFYL
                     LESKEINEQRSKSMANNQNNSSIPGNEIQISDSYLPF"
     gene            12896..13192
                     /locus_tag="IWM35_000912"
     CDS             12896..13192
                     /locus_tag="IWM35_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9918781.1"
                     /translation="MKIIDSYDQRTMQYLGSFDYTDENIINYVASLPPYQSVRLVDFA
                     SDELVLTTMGNFLDYVGDKKWLEKILSKLIAKQTGEEPINAIQLFNRYEQENND"
     gene            13207..13638
                     /locus_tag="IWM35_000913"
     CDS             13207..13638
                     /locus_tag="IWM35_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9918782.1"
                     /translation="MKRLKRSIFIVLALIDFVGTGCEAIDVQKAPLSEIKQAITKQDE
                     EQPLNTQVEIVNQNKPSFSEEDFSLENGTRQFFSDLDRLNRVGVASALLHKSRMPTEE
                     RDRLYIKPTGWKQKKMKNGDYLYNRCHLIGYQRATSFPISM"
     gene            13820..14125
                     /locus_tag="IWM35_000914"
     CDS             13820..14125
                     /locus_tag="IWM35_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9918783.1"
                     /translation="MKLDMPGRYKLLRVRLFLIKLTNLRMYGSITDLYSKDIRLRMQR
                     TLSKSKNVGHEIRDGRLAVYLSRVGKTCLIDSDESGNATKRFTEEIEQLAELNIRYF"
     gene            14529..15017
                     /locus_tag="IWM35_000915"
     CDS             14529..15017
                     /locus_tag="IWM35_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HAP9918784.1"
                     /translation="MKKTIFVLAIFICFGLLLVGYLLFNSNEMRTISTNDLLKKSELK
                     DKKKESLAFIMFTQEECEYCIAINPIIDSVNKETLNKIKKYDISVDFQSKQKCKIKFV
                     PTIVAYSKNKEVGRIDNELDNLYRKKQDGSIVIDKKKTKTLLIDWMKKMDKQGEYLYG
                     ED"
     gene            16351..16653
                     /locus_tag="IWM35_000916"
     CDS             16351..16653
                     /locus_tag="IWM35_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP9918785.1"
                     /translation="MTKLGITLDDNIYDEKILLSGGEKQKISLARALIKSASVIILDE
                     PFRNLDNETRDNLRDYILNTKKEVCYIIITHDHILDNFLDSNDEILYLNKEYDNNE"
     gene            16646..17674
                     /locus_tag="IWM35_000917"
     CDS             16646..17674
                     /locus_tag="IWM35_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HAP9918786.1"
                     /translation="MNSKKLQMGEIVRGPSQISIDVTYKCNFRCKHCYNASGGDNCFM
                     DNELTDDELLELTKDICNLKPYNICFCGGETLLRKKIVLSCATLLKEAGIPNIAIVTN
                     GSLVTQDVAKELFKAGINRFQVSLDGKNASSHEALRLYPNSFEKAINAIKLFRELSEE
                     VDVAFCPTSFNYNQLREVYQLVNTLKVKSLRIQPFMLSGRGMQNREIVPTVSQYRKLV
                     SDINYIKRNEDYTKIDWGDPIDHIIRHRTLLKDFSNFVSIRSDGGLMASPYLPIQVGN
                     IKCHSLSKYWRAGLYKVFSLDLVQDIATQVSSIQDMSKELKKGHIKWLNEDIEIDILD
                     EYKHDILI"
     gene            17730..17948
                     /locus_tag="IWM35_000918"
     CDS             17730..17948
                     /locus_tag="IWM35_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9918787.1"
                     /translation="MISEPQMNNMYESPTIMGAGEAAPRACSAIMVCVLLASAGVGIQ
                     AGAVYTYAVVATVAVAAAAVWKTVGLVG"
     gene            18019..18834
                     /locus_tag="IWM35_000919"
     CDS             18019..18834
                     /locus_tag="IWM35_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9918788.1"
                     /translation="MKYNNLKPKLLNENIEYIREENYGYLTIVPKNGTELKDNVFNPT
                     VRYILANCTGKNSVKKIANGLKKKFPSLEYEKFYQDVVGTIKFFKHYNIIDIGDYSME
                     EKKIYKIEQNLELELLNESHYEEIYNFITDHPESMYFHEDIYHQGNSKELEVVLRYNL
                     FSFTSEFYFLRHKERIVGMVSIIVNPFQNLSYSIVNYLICEPAYTTTILTTVLENINE
                     VSILEVKKLVIHLLNSTEDESLIPYLKDSHFFKEAVLSKEYREHDVLRYAYIY"
     gene            18898..19233
                     /locus_tag="IWM35_000920"
     CDS             18898..19233
                     /locus_tag="IWM35_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HAP9918789.1"
                     /translation="MILLILSLVLLVVSNLMKNNPPTKINSFYGFRTKKSMKNEKSWH
                     LAQKLLGDFSKKKVIPVFLMGIVFFSIEIYSLAILKNENFFVSVLMGESLILILILCL
                     IVHEVNKQL"
     gene            19657..19920
                     /locus_tag="IWM35_000921"
     CDS             19657..19920
                     /locus_tag="IWM35_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9918790.1"
                     /translation="MFIKYGADTVATMFTEEYQVDYYEVIDKCVHFVYEKVWNFMNFA
                     ISEEILRVQENRTGIFTRDRKLNLIAHYIRNRWSSIPNFNYKK"
     gene            20236..20666
                     /locus_tag="IWM35_000922"
                     /pseudo
     CDS             20236..20666
                     /locus_tag="IWM35_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347687.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
     gene            20805..21227
                     /locus_tag="IWM35_000923"
     CDS             20805..21227
                     /locus_tag="IWM35_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9918791.1"
                     /translation="MVKEEVLCPHQDFLYLIPANQETTQHYFTFLEKQNELVEALMES
                     QETVDYLLSLEFVQHPLEHSKEIYENFELFTSALIYLNHHNYELIPEHWQLLGSSKQQ
                     GKKASVPSLGKKELITIYQQMFEDQPELPIFYQLKKAG"
     gene            21372..21617
                     /locus_tag="IWM35_000924"
                     /pseudo
     CDS             21372..21617
                     /locus_tag="IWM35_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347587.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            21726..22682
                     /locus_tag="IWM35_000925"
     CDS             21726..22682
                     /locus_tag="IWM35_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9918792.1"
                     /translation="MLILANQASTFVQTQQLRGRGLRLGAQNKVTNIWHLAIGLPGVP
                     LSEQPDLKRMLRRLGNISGLTFESSPKIESGGDRFALPLEYTTTTMETYTQSMFYFSC
                     ERSRLKEQWSTALADGWHLSMPIVVRKVDQVKQKQETSLPIVVKNIEPLSFWSAIFKG
                     NLLEYFQIRRRQKLWEKECARKKSLAICVQTTLQRLGEVSDRKVIVAADQQEFRCYLE
                     NAGYREGQLFTEAMRELLAPLENPRYLIEGKKIVLTVPERFGRNKQIANLFLNELNQS
                     SLPKCQLVYTRNPEGRQKLVTYRMRQVFQTRDEVIIQKNIWT"
     gene            complement(23518..24189)
                     /locus_tag="IWM35_000926"
     CDS             complement(23518..24189)
                     /locus_tag="IWM35_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP9918793.1"
                     /translation="MTIKTSLQNQAYQSIRQQIIYADLEPGKKVSEKKLEETLSIGRT
                     PIREALIQLRHQGLVDTIPQSGNYISLIDLDLAKNARFVREHLERQIMLECCAKMNNQ
                     KKQVLETIIAEQEKAVSQKNARSFFHNDNLFHKACFEIAEREEVWQWLEDNNTHLERF
                     RWLRVKTEGLKWEKIMEQHHQLFKALTMQDPEEANFLTSLHLHLMLDEQNVVLTTHPK
                     YFKNI"
     gene            24506..26137
                     /locus_tag="IWM35_000927"
     CDS             24506..26137
                     /locus_tag="IWM35_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HAP9918794.1"
                     /translation="MVLIKDWQDSIEGLNKMQVQLPYFNVEAVKQEGQKHPVWLHFGG
                     GNLYRGFHAEIAQCLADQNELESGVVVCETFDEEVVEKAYHDYNNDILEIVMGEDGEL
                     KKRLLAVTADSLYCNPKNITDYQRVVKYFEDPTLQFTTFTITEKGYSLKDSKGQFFSV
                     VEADIKSGPTNPQHTMSIVTSLLYSRFNAGELPIAMVSTDNFSQNGQRFQDSIFMIAE
                     AWQKKGYVSEKFIAYLKDKEKVTFPWSMIDRITPNPSESVKMDLLKAGFEDLDIIHTA
                     KNTNIAAFTNTEKVHYLVIEDSFPNGRPKLEAAGVIMTDRETVDKADIMKVTTCLNPL
                     HTALAITGCLLGYKSIQAEMQDDDLVGLIKGIGYVEGLPVVESPGVIDPKQFIDEVIN
                     KRLPNPYIPDTPQRIATDTSQKVAIRYGETIKKYVEKDGNAENLTFIPIAIAAWLRYL
                     LAVDDQGKNFVPSPDPLLVELQEKFSVLKLGEQDPAKIHCAVQEILENKEIFGLDLYQ
                     VEIGKKIEKIFCEMLVGPQAVRKTINKYITEVGGK"
     gene            26140..27219
                     /gene="uxuA"
                     /locus_tag="IWM35_000928"
     CDS             26140..27219
                     /gene="uxuA"
                     /locus_tag="IWM35_000928"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAP9918795.1"
                     /translation="MEMTFRWYGANEEKISLQEIRQIPAMKGIVGTLMDIPAGEVWPR
                     ERIKALKDEVEANGLTLKVIESVNVHDDIKIGLPTRDQYIENYKQTIRNLAEFGIEVI
                     CYNFMPVFDWVKSDLDYHLDDGSSTLAYISKNIPTDPEEIIRKVENSTNNFELPGWEP
                     HRLSQIKQLLEAYKEVDEAKLRENFAYFLKAIIPTCEEVGIKMAVHPDDPPYSIFGLP
                     RIVKNRDDLEWLCNVVDSPSNGITLCTGSIAERPDNDVYEILSEFCQRDRIPFAHVRN
                     IKYLSDNGLDFYEAPHKSEYGSLDMYKILKTMYDNGFNGYIRPDHGRMIWGETGRPGY
                     GLYDRALGAVYLNGIWEALEKGASK"
     gene            27293..28720
                     /gene="uxaC"
                     /locus_tag="IWM35_000929"
     CDS             27293..28720
                     /gene="uxaC"
                     /locus_tag="IWM35_000929"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HAP9918796.1"
                     /translation="MFLDKNFLLENEIAKRLFHKFAEHQPIIDYHCHLDPACLLENKQ
                     YKNLTQVWLNDKGAGDHYKWRLMRANGIEEELISGAGNDYEKFLAFVKTVEKAPGNPI
                     FEWSHLELQRYFDIDLTISEKNAPEIWRRANEKIQTDSYRPKELLKMMDVKAICTTDD
                     PTDDLRYHTELSKQDNSFKVLPTFRPDKVWSIKSSGFSNYILKLSQAAGIPIKNFNGL
                     KQALSQRVDYFHQIGCRLADHGLNTYFYEIADEKILDNLLIKALAGKTDFHLSEIAQF
                     VTGIQLHLMSEYTRYGWTMQMHLNALRNNSSILKEQIGVDVGGDSMGDQASLTQNVVA
                     LFDTAEKSGCLPKTILYSLNPTDWLPLATAMQSFQGGMIQKIQLGCAWWFNDTAEGMR
                     QQLTTMAQQSLLSNFTGMLTDSRSFLSYPRHEYFRRVLCQLLSEWVKAGRIPDDEMYL
                     SQIVENICYRNAYNYFNFFEIESRK"
     gene            28804..29451
                     /locus_tag="IWM35_000930"
     CDS             28804..29451
                     /locus_tag="IWM35_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HAP9918797.1"
                     /translation="MKKVEILSKLEAAGVIAVVRGKNKDEAIKAINAITAGGIKGIEL
                     TFTVPNAEEIIKEIAKKYADQKDVIIGAGTVLDAFTARIAIMAGAEYIVSPSFDRQTA
                     EICNLYQIPYLPGCMTITEIKEALTAGVEIIKLFPGSAFGPSVVKTYQAPLPYVNIMP
                     TGGVSLDNLEEWFEAGVVAVGVGGNLLAPVEKGDFEEVTRIASKYIDKFYEIKNY"
     gene            29580..30956
                     /locus_tag="IWM35_000931"
     CDS             29580..30956
                     /locus_tag="IWM35_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP9918798.1"
                     /translation="MERKFGLRDKIGYMFGDFGNDFTFIFASSFLMIFYTKVLGISGA
                     TVGTLFLVARFIDAVTDITMGRIVDGSKPSKTGKFKPWILRMSGPVALASFLMYQTSM
                     ASASMSIKVIYMYVTYILWGSIFYTSINIPYGSMASAITSVAEERTALSTFRSIGATL
                     AGMFIGVLTPIFIYTRDSSGAQIVRGGSTFTIVAGVFSVCAVICYVICYKLTVERVKS
                     DESQLKVSLLKSLKGLAKNRALLSIIAAAIFLLLAQLLTMSMNNYVFPDYYNNAQGIV
                     LMNFINPILMLVLVSPVSLIFSKKYGKKELASVGMFFSAIVYLLLFLIKPSNMYVFLV
                     LTSVGYMGLGIFNTVIWANITDVIDDQEVKSGQREDGTIYAVYSFARKIGQALAGGVG
                     GWTLSIIGYDSLAKVQSGEVLQKLYNSSTLIPAVCFFVVGLILFFVYPLSKEKVLENY
                     NLLNQKHN"
     gene            30967..31761
                     /locus_tag="IWM35_000932"
     CDS             30967..31761
                     /locus_tag="IWM35_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAP9918799.1"
                     /translation="MNSWIFCIDSDGCVMDTMTYKHNLFFGPIAAEIFDVHHREKFLQ
                     DWNNINLYSETRGINRFLGLVKTLEKNDVPSINRLKHWVSVTKELSNPSLKREIEYSQ
                     SEDLLKALKWSEEVNRQISQAKGLDKPFLSARDTIKALKKYGKIIIVSSANKEAIQEE
                     WERHELLSLVDELCCQDKGKKEDIIRSVIENGCDLDKILMIGDSPGDLEAANKNKVFF
                     YPILVNKEKESWENLRTDILFKCLSGEYIDIEQKYIDTFWKNLTNK"
     gene            31774..33540
                     /locus_tag="IWM35_000933"
     CDS             31774..33540
                     /locus_tag="IWM35_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-hexosaminidase"
                     /protein_id="HAP9918800.1"
                     /translation="MTIKVDLRKKPYHLDEDAISWIEETIDAMTLEEKIGQLFVNMGS
                     SRDKNYLSSMVNQYHIGAVRYNPGTADEVYDQNYILQTESKIPLLIAANTEAGGNGAC
                     TDGTEVGVEVKIGATNDAKYAYELGRVSGIEASAIGCNWSFAPIVDINRNWRNPIISS
                     RSFGQSPDKVLELSLAYMKGIQESGILPAAKHFPGDGIDERDQHLSFSINSLSTEEWD
                     NSFGKVYSGLIEAGLPSIMAGHIHMPAYSKHFNLDLKDEDLLPATLSKELITDLLRGK
                     LGFNGLVVTDASHMLGMTGAMKRSELLPTSIAAGCDLFLFFNDPDEDFNYMMEGYYKG
                     IISEERLRDALRRILGLKAKLGLHKKEKNKILLPKQEAMEKIGLIENKSIFKEVADQA
                     ITLVKDKQDIWPIVPEKYKRILLVDVHGVKGGFGEVVAGNRPQAIDLLKQILSAKGFE
                     VEKYISPEEELKGLSQEEKIKNIAGIYAAKRPIKDLTEKYDLIINIANVNPGTVQRIV
                     WPASKGTPDIPFYIHEIPTIFVSVQCPFHLADVPQVKTYINTYDGKQETLEILVDKLM
                     GLSEFKGISPIDAYCGLKDTFI"
     gene            33568..35376
                     /gene="uidA"
                     /locus_tag="IWM35_000934"
     CDS             33568..35376
                     /gene="uidA"
                     /locus_tag="IWM35_000934"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003479936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HAP9918801.1"
                     /translation="MLYPIMTETRQVIDLSGLWRFKLDTNDDLQEELALEPMVEKESY
                     PISVPSSYNDLFETENIRNHVGWVWYEKEVILPKKLLEERLVLRFGSATHEAKVFLNG
                     KLINQHKGGFTPFESEINQFIKDGSNRITVAVNNVIDDSTLPVGILKETIQEDGDIKK
                     ENLVNFDFFNYAGINRPVKLYTTPKDYIKDVTINTKIIDSDANVEFNIDTEWTGEILI
                     AIYDEEAQLIAEAKGKEGVIQLKNPTLWEPLNAYLYSMKVSLINENKVIDTYTEEFGI
                     RSIQVSNGKFLINNKPFYFKGFGKHEDTYINGRGLNEAVNIKDFNLMKWIGANSFRTS
                     HYPYSEEIMRLADREGIVVIDEVPAVGLHLNFMATLLDDNVEKHDTWKEIRTENAHKM
                     AIKELIERDKNHPSVVMWSIANEPDSDSEGAKEYFEPLVELTKELDPQKRPVTIVTYL
                     KSTPDICKVGDIVDVLCLNRYYGWYVSGGDLKHAKYLLKKELDGWMERCPDTPIMFTE
                     YGADTVAGLHDTVPTMFTEEYQVGYYEANHEVIDKCDNFVGEQVWNFADFETSQGILR
                     VQGNKKGIFTRDRKPKMIAHYLKKRWSSIPNFSYKK"
     gene            35548..35949
                     /locus_tag="IWM35_000935"
     CDS             35548..35949
                     /locus_tag="IWM35_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAP9918802.1"
                     /translation="MQQNYNEKSIQHQFDAYCKKVLRNETKSIRKRNINITDNEEPLT
                     NSNEGKYSQTHFDEQDVYFLFGMEILISDQKLSAAIDHLSEIRRKVILLYYFAGFNDT
                     EIGKILNMSTSGIWYQRKKAVEQLKMGYDLW"
     gene            35943..36188
                     /locus_tag="IWM35_000936"
     CDS             35943..36188
                     /locus_tag="IWM35_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002412373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9918803.1"
                     /translation="MVDNPKKRQYPLVPLSIIQSALEEDPISIKMILKHYHSYIAKLC
                     LTNGFNEAGQFITYVDEYMLRQLEIKLIEAILKFKIN"
     gene            complement(36512..36733)
                     /locus_tag="IWM35_000937"
     CDS             complement(36512..36733)
                     /locus_tag="IWM35_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9918804.1"
                     /translation="MRNEIANITIIRETLQNHTANEISKHTGLNLSTIKKLKSGERSI
                     EKLNLHDAICLTEFGLKNNRKNVEINIWK"
     gene            complement(36895..36967)
                     /locus_tag="IWM35_000938"
     tRNA            complement(36895..36967)
                     /locus_tag="IWM35_000938"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(36932..36934),aa:Lys,seq:ctt)
     gene            37043..37273
                     /locus_tag="IWM35_000939"
     CDS             37043..37273
                     /locus_tag="IWM35_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9918805.1"
                     /translation="MDFQDYEKIFESYPSILKITDLTKMLNIGKNKAYNLLKDRSIEN
                     IKIGARYKIPKKAVIEYILKIQDEGKNHAIIK"
     gene            37317..38486
                     /locus_tag="IWM35_000940"
     CDS             37317..38486
                     /locus_tag="IWM35_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP9918806.1"
                     /translation="MKLRANIIEKRGTLQVVVSYKDSNGKIRQKWKDTKLKKKGNKKR
                     AEKIRDEFLLELESQLAMECECANPNLLFYDYLLNYLEVSKKQVAFNTYVGYKHYVHN
                     RIYKFFYPKKIKLNRLKPYHLQEFYQYMLNDNCTANTVIHYHAFIRKALQEAVITELI
                     STNVADKVRKPKKKQFVSQVYNQQEILKLLDILSEEKLYLVVLLTAFYGLRRSEVLGL
                     KWSAIDFLNKKIMINHVIIENPENMSQLIKKDQTKNSSSYRTLPLVETIERALVNQAK
                     WQQDNRILLGDDYILEDNEYVFTMEDGRLMKPQYVTHRLSKLIKKNGLKKIRFHDLRH
                     SNASIMLDSGQNMKSIQEWLGHASYSTTANLYTHLTAGVKDRAAEALENAFGFKE"
     gene            39060..39629
                     /locus_tag="IWM35_000941"
     CDS             39060..39629
                     /locus_tag="IWM35_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HAP9918807.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            39616..40476
                     /locus_tag="IWM35_000942"
     CDS             39616..40476
                     /locus_tag="IWM35_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAP9918808.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITPEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            40666..41370
                     /locus_tag="IWM35_000943"
     CDS             40666..41370
                     /locus_tag="IWM35_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9918809.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            41375..43210
                     /gene="walK"
                     /locus_tag="IWM35_000944"
     CDS             41375..43210
                     /gene="walK"
                     /locus_tag="IWM35_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HAP9918810.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVILSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            43207..44523
                     /locus_tag="IWM35_000945"
     CDS             43207..44523
                     /locus_tag="IWM35_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9918811.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            44524..45393
                     /locus_tag="IWM35_000946"
     CDS             44524..45393
                     /locus_tag="IWM35_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9918812.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            45448..46257
                     /locus_tag="IWM35_000947"
     CDS             45448..46257
                     /locus_tag="IWM35_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAP9918813.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(46360..46989)
                     /locus_tag="IWM35_000948"
     CDS             complement(46360..46989)
                     /locus_tag="IWM35_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HAP9918814.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAASDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            47159..48451
                     /locus_tag="IWM35_000949"
     CDS             47159..48451
                     /locus_tag="IWM35_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP9918815.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQHLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            complement(48527..49189)
                     /locus_tag="IWM35_000950"
     CDS             complement(48527..49189)
                     /locus_tag="IWM35_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAP9918816.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            49414..49698
                     /locus_tag="IWM35_000951"
     CDS             49414..49698
                     /locus_tag="IWM35_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HAP9918817.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            49749..51374
                     /gene="groL"
                     /locus_tag="IWM35_000952"
     CDS             49749..51374
                     /gene="groL"
                     /locus_tag="IWM35_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HAP9918818.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDATIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
BASE COUNT        18078 a         7581 c        10408 g        15393 t
ORIGIN      
        1 gagggggcaa gtgtggttgt ccacgaagta gtgattgtgg acaaaatacc gaaattattg
       61 ttagaacggt atggcatttc tcatgagtaa tgcaaaattt aatccacaag cgttccaaac
      121 taagccaccg aaacaacaga aggaaaagaa acaaaagaaa cgttttttgt tccaaaaaaa
      181 gaaaacagaa ccgtcagatc gaatgatcca attttcgcaa aaaagtgcca tgagtgtatt
      241 ttcaggcgct tttttttgtt tgtttctgtt aatgggtgtc gtgctcctcc taaactttgg
      301 acgagtggat acattaacgc gtttagcagt gaaaaaacaa gtcaatccag aaacattgtt
      361 aactgaagtc aaccaaacct tgtcttctac ggaacaattg aagtacgagg gacaacattt
      421 tattgaaagg ttatttactt attcgccaga aaaacgaaac gaatgggagg cttccgtcaa
      481 tcattattta gcacaaggat tggaagcgtc tgatctaggc tttgtaacga cggctaccaa
      541 tcgagtagcg aaagatgttc gctttattaa actagaagaa atcgatttga cacaagcaat
      601 gtatcgccta ttttatgagg ttacttttac agaaggagaa cattggaaaa gcagtcaact
      661 ttcttttcca gtcagttatc aaaacaatgt tttaaaagta cttgatggcg ttcaattttt
      721 gaatctttct gagagtaaga ataccaattc tgtgaactat caggaaaatc gattttattc
      781 ttctgggcaa gcggtcactg aaagcgaaga aatcaagctg gaagcatttg tgaaacggtt
      841 ttttgagtta tatgtcaaaa atgatgaaaa attggcattg atttctaacg aaaaagggtt
      901 agaaggcggc gaattgatca atgtacaact ggatcatgca gtaaaaaata aagaaggaaa
      961 ctactttttg caagggaagt atcgtttcta ttttgagaaa ggaaacgagt tccaatcaaa
     1021 cttttcaatg gaagtaaaaa caagtggcga tagttatttt gttactacgt taaatgaata
     1081 aaatgaactt agaggagaat ggaaatgaaa ctttttgatc ttaaatcgtt atttgatgat
     1141 gtatctggac aagtcatttg gctattggca atcgttgcga ttgttctaat ggttggtgct
     1201 tatgcgaccc aaggctttgc gcgggcagcg tttgccatta ttggcgtctt tattttgata
     1261 atgctcgtct tattgttatc gaatgccgtt gatattggtg aatggctaaa gaacattatc
     1321 tttattaagg atagtgaaga gaacgtgggg gtaacgcaaa tgattgtgcc ctggatacaa
     1381 ttatcataag aaaaagacaa actgcaaaag cgatcagaag attagaactt ctggtcgctt
     1441 ctttttttgt aaaaggaggt agagtgaaca tggaatacaa ctatacaaag gagtttaagc
     1501 accctcacaa aatttacggg attaaaggac aaaaaattcc ctggatttct aatggcattc
     1561 gcatggaaca attagtcata ggtggggtat ttctcttcat tcttttgatc ctatttttga
     1621 ttgcgtatgt gaatcaaatt agtttcttgc agacactgtt tcgaaagtcc tggttgatta
     1681 tgatttttgg gattggtgtt ctcgtttgga cattgttttc aatgaaatgg gacaataaac
     1741 actttttcga gtatttaatt gggcgaacgt cctattatca gaaacggaaa caacgctttg
     1801 aacatgaaat gtatgtgccg tttttccatg aaaaagtgac gtattcccta cacaaatgga
     1861 aggagtgagt cgagtaaatg gcgtttttgt ttcttttggt cgtctttatt atcgggattt
     1921 ggcagttcaa aaagaaaaaa acgaagcttc ctcgattaga atttccgata gacgccatta
     1981 tccgaaattt tgttttgacc aaaaatgatg atgtgtgggt aggctatcaa ctttcttccc
     2041 aagtatttcc tttaaatgac attgattttt tcaagaacta tcttcaagat ggcgaaggca
     2101 tacttggaca tgatgaatat gactatcatt ttatcaatat tccacgtcaa tttaacctag
     2161 atacgcacat tgaagaaacc attcgagatt tggtaaaggg agaatgcgca gatcttggga
     2221 aagtgtactt tgaacaggca ggaaagattt taaaagacga agtgcaatta aacgagtatc
     2281 gaacgtatct gtttgtgcgt ttcactacag aaatgaaagt gtctgatccg atcgaatatt
     2341 ttcaattgct taagcatgtt tttaaaaagt ttatctatcg tattacggga caacgagttc
     2401 ctataagtag tgtgttacca acgtatgaaa cattagaaaa ggagctctat acggaattgt
     2461 ccaattataa gccgatagag cgcttatctg cccaagagat tggacaaatt tattattatt
     2521 ttttccatcg atcagaagaa aaattgcctg aaagacagct tacgccaatg gaaatttgcg
     2581 agggcgtcat ttcgaatgaa gcaggataca ttgcgattga acagttgaat caaacgcatt
     2641 attcgacgtt tttaagtcaa acggacgctc ctttagccat gtatgggagt agctttattc
     2701 aaaatcttca ggatagctta tcttttccaa ttgatacgca tattcgactt cgatttgagc
     2761 attcaaaatc tgatgaacga aaaattaata aaatggctgg acggatttat gaacaagaaa
     2821 aagaatatga gaccgttgac ggcattttag attcagacga tgtattaaat tttggtacag
     2881 aacggttaat ggatgtaaaa gataagctga agcgcaaaga aaaacgattg tgttattcta
     2941 ccgtcacgct ggttgtatct gcttcttcaa aagaagaatt ggaagaacga gtcaaagata
     3001 ttgaatttgc gattgagtct acagatttca aattttatcg atcggtagcg gatcagctaa
     3061 cgttgtttaa tcaatgctta attggaagta cgcaacgttt tcgtagttac gagcaggtcg
     3121 ctacgacagg ttttgtggca gattatggct tggacttaaa gaaagaagta ggaaataaga
     3181 aaggatttcc tttaggacgg attgttattg caaaaaagtt taaatctgcg aaagaagcgt
     3241 tagcgctatc tagtaaacta gtatggtatt atcccaattt aacaaagaaa agtattgaag
     3301 gcgcggaaca taccaatgga aatacgttga ttattggtcc accaggtaaa ggaaaatcgg
     3361 tccttgtaaa atatattttt ttatggctga tgtttttagg tcaaaaaatt ctttatattg
     3421 atccgaaaaa tgaaacccaa aagttctttt taaaggcact tgaaaaattc ggacacatac
     3481 cagagtttgt ggctatgtat gaacgaatta attttatttc gttatcttca gatgaaagct
     3541 gtcgagggat gcttgatcct ctattgtttt taccaaaaga agaggcaatt caaaccgcta
     3601 aaaatgtgtt aggaatgtta ggagaagtag ataccaatcg agaaacagcg agccacaaga
     3661 aaacggtgat ccaagacgcc atcgatgtag tttacaccat gccaggtaag cattcgttaa
     3721 gtcaagttat cgaagaaatt cgaaaaagtg acaaagaact agctaaattg attttagggc
     3781 atagcgttgg tttaggaaag attctcattg ggaatgagca cagtacgccg atacgttttg
     3841 attcacaaat caacgtgtta gggacacaag gaatacgcat tccgacacaa aaagagattg
     3901 attcgggtcg attaaatagc gaacaacttg caggaatggc aattatggaa gtcattatga
     3961 agtttacgta tatcttttct acaaacaaag aagaagacgc tgcgattatt tatgacgagg
     4021 caaaagggta tgaagatacc gcacaaggaa gctatttgat tgatgatagc ttgcgaaaag
     4081 gacgtgcgaa taatacggat atttaccttg ttgtgcaagc gtttaaagat tacgatcaag
     4141 aagataaaaa agagttaatt tcttacaaat ttgcgtttcg tccgaaacaa aaagaagcac
     4201 gagagaaact attgtccttc tttgatatgg aagtgaattc agccaattta gaaatgatgg
     4261 aacaattaca aacaggaacc tgtttattcc aggatcattt gggaagaaat caaccgattg
     4321 ccattgatgt gttgtttgat agttggttat tggctgtttc ttctactgaa aatcaagatg
     4381 aaaaaattca agcaatgtta gaaaatgaaa aacaacgata aaacaaaaaa gaggtggttt
     4441 ttttgcgaaa aaaatctctg aaatggtgga cgctcctttt attagggggt ctttttttct
     4501 gtctacaagg ggctactgtt tctgccgatc aaatgaatga tggtgcgggt gtcactgaaa
     4561 ccgatcgttc tgaaggagag aatgccaacg ataaagtaga agtaaaagaa gtaacgactg
     4621 atgtggatca attgtataaa aattacaaaa agaattcgtt tgaattgatg accaatgact
     4681 atgagtcaat gaaccttaaa aaaaagctat cgtacaatgt tgcaggagtg atgaaaaatt
     4741 ttgtttggag tggcgtaaaa atgttaggag aattcaatgc ttcaatggtt gaatttctct
     4801 ttgaattaga tattatttcc gccattcgag aacccattca aaacttaaca caaaaaattg
     4861 cggacaatat gttaagtatt gcaggaacta ttggaatcag tttttcggca attatgatgg
     4921 caatcaaata tgctggagag gctcgaatga aacaagcact ccgtgtcttt cttatgacga
     4981 tccttatttt tactgggtta agcgtattaa aagattctgg gacaaataag aatctttttg
     5041 atgttctttt ttctttagat tctacagtgg aaacggcgtt cgtgaaggtc aatccagtgt
     5101 taacacctga agaagaaaca aattatgaaa caaaagaaac agaagaggta acagcggata
     5161 aaaaagtgga aacggcaggc aaattgatgg cggctcgggt cttttatagt aatgtttatg
     5221 aaccatatct gttgttaaat tatggcacaa ccaatgtaga cacaattcgt gagaagaaag
     5281 tcaaatatgc tggaaaagaa tatgatcgaa tcaatctttt attggacaat gatttaggta
     5341 caaaaatagg ggagaaaatt catgacaaag tcacagagta tgaagcagat acactgaaaa
     5401 acaagaacat acaatattac aatagttgga aaaatacagc atttgccttg ttttattttg
     5461 tcgtgaatct gattcaaacc gttgtttatt ttgtcttaag tatgtttcga ttgattattg
     5521 gaactatgca actattgtta gtaccgatgt tgccgttctt acttttttta ggattgtttc
     5581 ttgtcacaat gaatgtgttt ggtaactatg ccaaagtttt tggtattttg acgtttttaa
     5641 aaggaatgac aggttttgca tgtatccttt tttcttcttt tcttagttac gggttccaat
     5701 taagtagtac acaagacgat ccatgggaaa aaattctctt gatcattgtt tatttgtttg
     5761 cgccgttggg aatttatttg ttccgaggct ttttaggcag tctatttact ggaagaatgg
     5821 gtgttcgagg agcgttagct tttgcgggaa atccatttgg ggcagaagca aaattgagaa
     5881 gggaagccaa acaacggttg aaagaacgca aagaagcgag aaaagcacag aaacaaaaag
     5941 aaaaagaaaa cgcacggaag agacaagaac gtcaacgaaa acgtgggggt ggctcagacg
     6001 ttcgtatgcc acaaacggcg aaacaagcca cacgaagtgc ttttcggcgt gaatccgcac
     6061 aacaacctcg tcataaagca ccaaatcgta tggaacgtat ggcacaaaaa gcaagagact
     6121 tgcatgatca agcgaaacaa gcagaagagc aaagtcaaga acgtatgcaa cgaacagcaa
     6181 tggagaaaca ggcaagaaaa acagccgatt tattacaacg agaattgcaa ggcaatccta
     6241 gtctaagaga aagaagtaga aaaggtgcgt ctcgtagtcc gtatcgcaat cagggttctg
     6301 caatggtggt gtcattagcg aatccaaagc aaaaacacaa gtcttatggc cagaagcata
     6361 cgcctagaac agaattgaac gatcgaaaac gtcgtcaggg acaaacaagt cgtcgtcgga
     6421 aggaggcact tactcccgta acaaaaacaa gttcgatgaa aacccggaca gttgcaggac
     6481 aaaaacatgc ggtacaaaaa ccactaagaa aagggaatgt accacgagca acaggttcta
     6541 ccacaccgaa atctgttcga cgtccagagg gggttcggga acatatgtca gcagtcaatc
     6601 gagtaacagc acaaaccaaa gcgccttcaa ccgtatctaa agcatactca accatgaaac
     6661 aaacaaaatc accaaaacaa gcaccgaaga cacggagtga gtttcgagca actcaagcaa
     6721 aaatgaaacg aaaacgagaa aaacaaccga tttatcgaag agaaatgaaa caacgaaaac
     6781 gataggagga aatcgatagt atgatgaatg caccaaaacg accgacacat tttgagcaaa
     6841 tgttttctgt cgatcgcaaa agaaaacaag aaaaagaaac gccaaaaagt cggtttcaac
     6901 aactttattt tatcgtcact ttattaggtg tttgtctata tccatttttt attgtaggca
     6961 gtattccttt agcgattgtc tttatgatag ataaaaaaga gacacgtgca catatttatg
     7021 actttgagta tgagagcttc ttaaaacgaa gaagctctct tttttttatt tttgcgttag
     7081 gttttacgct attaaatcta ttttgtttta tttttgttct tcctagaggg tatttgtcgt
     7141 gttacttatt atttccatta aacttgtttt ctcatgcgtt gcagtttaat ggacaaacga
     7201 tttttgccct ccttttaggt gggacaggtg caggaagttg tttgattgcg tatgcgtctt
     7261 ttattgaaaa aagaaaagtt gtttcaaaga aagaacggca aacagctttg ctaaatagta
     7321 aagaatacaa ggaacgtcgg aagaataaat ttcaggagtc ccaaaaatat acagcagagt
     7381 atcagcaagc gtaccaggta gcaattgtca taaaagacgt tgctttgcgg aataaacaac
     7441 tggcttcatt agccaaagtg attctattag gaacagatga gtatgggctc ccttatgtga
     7501 tgaactttag tgaattgaac caacatgtga ttattccagc gactacaggt tcaggaaaaa
     7561 cgacggtgat ccaattacta gcagaacatg cgtgtaagtt tcgtatcccc gttatcttag
     7621 tcgatgggaa aggagcagga gagactttac aagcgttaaa agaagtggca gaggcataca
     7681 atcgcccgat tgaagtcttt gtggacggtg gcgatttgcg ttataatcct gtaaaaaatg
     7741 gcaatgatat tgccattcga gataaattaa ccaatctagc agaaacagaa agcgtgtatt
     7801 attcgacagc agccaaagcg ttattacagg taacgattca gcttttagac gagttccaaa
     7861 agagccataa aattgaaaga aatctagttt ggttacaaca ttatttattg ccacgaaacg
     7921 tcttacattt gtttgcggat cgtattcagt tgaaaaatcc agatttattt aaagaaaaag
     7981 tggtcgtaga aaagaagaaa accacgaaga aaaaaacgaa aaagaatgaa gcgatggaca
     8041 aaacagaacg tgattttgcc gatgtggaac agttaagtga agagcgaatc gttgaacatg
     8101 atcgtaaaac cgttgactta gaaacgtatt atctattatt aaaagaaaac atttctctca
     8161 tgacagaaat cgagcaaacg ttgtttaaac ggttgtttgt tcgttacgaa cataagaaga
     8221 acccgtttta tttgtatgcg acttctgaga atttacagac caatatcaat atgttattgg
     8281 atagtgagct tggtgaactc tttgatactt ctggaggaaa aagagaatta gatattcaag
     8341 aagttgttaa agaaggaaag attgtttata tctcgttaaa cggattgatt tatagtgagt
     8401 ttattcggac attggctcaa atgcttgtgg gagatattaa tttctttgcg tcagaagtgt
     8461 ataaacaagc aggagaaaca gccaatcgct cgaaagtcct agtaatattt gacgaaccag
     8521 cttcttatct aaatgataac cttatagact tagtcaataa gggacgaggc gctggggtgc
     8581 atgcggcgta tgcccctcaa acgcttgcgg acattgatag aattgatcct gtccttcgaa
     8641 aacagttgat tgggaatgtg aatacgttct ttattgggaa gacaaatgac ccgacagaaa
     8701 ttgattattg ggcagattta atggggacag aagaagacat tgaattaacg gaaatgacga
     8761 cacaagaagc aggttattct gatatgggaa aggttgattg gacaggagaa agaggcacga
     8821 aacgagacgt agaccgcttt aaattcaatc cgaatcgcat tcgagcattg agaacaggcg
     8881 aatttgtggt gtatcgaaca gctgaagacg tccaggaacc accgcgagtc gtctatattc
     8941 ggaaaccaaa tttggagaaa caatatgcga aataagaaag ttattgttag gatacttatt
     9001 gttagtttcc tcttatttgg ttgttttaaa ctgataaaaa ctaacgaaga atcaaatgga
     9061 aaactaatca atagtaattt agaagaagta ctagaacttc aaaaaacgaa tcaatcattt
     9121 tttgtgtatc tagcaagaaa tgattgtaaa aaatgccaag aagttgaatc tttcttggaa
     9181 agatacgaca aaaaattctc taggaatgta tatcgaattg aaacaagaaa agaaccaaga
     9241 caagaagaac ttcaaacttt tttacaacgt taccatgtta aaagtgtccc aagttttctc
     9301 gtgattcatg atagaaacgt caaaaaaatt gagagaaaaa cattcttttt tgaaaagaca
     9361 ttgaatcaat ttcttcctaa tttttagtga aaattgaaag agaatacgtt gaaaaaaaca
     9421 aaaaattgtt atgagttcaa atttttttcg atcgtatttg tttttctttt gaaacaaatt
     9481 cactcatttt ttgagcaaaa atgtttcaaa atttgaacaa aaaccaattg aaaattgaag
     9541 caaatttcac tcgaattgaa tcgattttga gctaaaatca atagaaaaaa taaacgtatt
     9601 ttgtttgaaa tccgtttatt ttttttacgt ttcagtatta gtaaggatta tttgagggtt
     9661 atttagagat tatttaaagg ttatatgtaa taagtggggg gagggctgtc ggcgcttggt
     9721 atgaaaggca ttctccgact tccaggtttt tgaccatttt ctctcaacgt ggaaaaaccg
     9781 ccacttatga gagaaaatgg tcaaaaacct aagcgggaag tctccgaata acataccaag
     9841 cgccgacagc caacagataa agaatacact actaaaaata gtagtagcat aaaatttaaa
     9901 agggaaaaaa tcaaccccta ccccagtctt ggggtctcaa aactaactcc cccctaaccc
     9961 cctctttcag cctcttttga aagaggggga gaatcaaccc cacggccaac gccccagtgt
    10021 tcttggggct gtccagaaca gaaacttgcg agagagtggg ggtaaaacaa gtgtagagga
    10081 aacttgaaga cttgttcgaa cagaaacttg cgagagagtt ggagtaaaac aagtgtagag
    10141 gaaacttgaa gacttgttcg aacacaaact tacaagagag gggagtaaag cagttgcaga
    10201 ggaatcagga agatttgttc gaacagaaac ttgcaagaaa gcattattaa attgaaggtg
    10261 atgaaatatg aagtatttat tgcatggcta ttttattgat tattctactc gcaatagtag
    10321 tactttagcg attctatgtt tgcttcatga attgagagtg gttactagaa aacagctata
    10381 caaactattg aattttgaca gatcaattag taaacaagga gtaacgaatg ctttagtgag
    10441 attatatgaa aaacagttaa tcgatcgaaa aaaatatgga acagaaatgt attattattt
    10501 gacgaaagag ggacatcaaa gcataggtgg gtattatacg ttgccgaaag ttcctgaata
    10561 taatttgaat caccatttac aaattaacaa ttatttgatt aaaggaatgg cattggcaaa
    10621 agaccaccca catttaaaac ggattatttc agaaagacgt accgtctatg aaacgaagga
    10681 tttaaatcct aagacaaaag gaagaaaatt ttttgtgcca gattttattt ttcgttttcg
    10741 agacaaagaa aatcatgaga tcaattggtc ctttgaaatt gagctaacgt tgaaaacacg
    10801 tcgtcgctac cgtgaagcga tttttccgaa atatattcgt gagttgcgaa cacgtcctga
    10861 atcacattta atttatgtga cacctagtgg cgcaattcaa gaagaacttc atgcgtttaa
    10921 gcgtattttt ttcaagcgcg aaactgaaaa agatgcgagt caacaagaat tgtttgagcg
    10981 gttgcatatt ttttcagctt tagagtttga agagggatta aaagcattat tagagaatga
    11041 tccagcagtc aattggcgga cagtagaacg agaaaaaatt tatccgaatg gcgctgttca
    11101 aagtcgtttg aatttatttg aagaatcaac tgaaaatgag gaaaaaccaa aagtgaagtt
    11161 tacgcctgaa gtattgtaaa agagggtgtt cgcttcgctc tttttttgtg ccagtttgct
    11221 ggcaccgttc agacgcaagc gtctgaaccg gctcgttttt aggaaggact aacttgtttg
    11281 atttatccgt tgtcaaagaa gtggacgttc gagagtccct aatcgtatag cagtcattcg
    11341 gcgagtataa cctaaaacat aacctgccaa ttgccaaggg taaaatgcac gctgcgcgcc
    11401 cttggcaatc gtcagaacat gttttcgagc cggttatgcc gaacgaccgc tatacgaaaa
    11461 gggcctctaa acagaagtcc actttgatcc ttgacaactg aaacccaaaa tcaaacaagc
    11521 aggccttcgt ctaaaaacga gccgaatgaa gaccttgggg gttgcacccc caaacccccg
    11581 tgcccccgac acactaccga aaaggtgggg gtgggtggcg aaaaaatcaa ataaaacatt
    11641 ctattcaaga aaggaagaaa caaccatgac aactaaaaac gagcaactga aagaacaggc
    11701 aagaaaagag ttgcaagaaa gaggtttgat tattgagggg agctttgaag gagattttga
    11761 aacctatatt ggttgttatg caagaccgat aaacaaacct acagccttag acccaatgaa
    11821 cgaacaagaa gtaaaagaac aagaaaaaca tgcagtgaat ggttttccgc aagatttcac
    11881 tgaatggtat gagtgggaaa ttaacaacgg gaagcttgaa aacttcttat aaaagtcaga
    11941 tgacgtgcaa agagaatgat tctctttgta cggtaaaaat ataaagaaag aaggaaaaag
    12001 aaaatggcaa aaaacacacc ttatcaagta ggagacattt tttatagtag ttggggctac
    12061 gaacaaacga acgtgacttt ttggcaaatt gtgaagttaa cagaaaaaac agcatggttt
    12121 cagcctattc aaaagaaaac ggtcgagcat tattcgtcca tgagtggaaa agttgttcca
    12181 atgaaagaca agtttataga gtatccttta gcaaaaacta aagacagtat tgtaaaaaaa
    12241 cgaattgatc cagaaaccta tcctgatatg ttatgtggtt atcgtagttg ggacacgttt
    12301 catagatata aaaatcaagc tgtttatgaa agtagttatt attaagggga tagtttcccc
    12361 tttcaaaaat aataaataga agaatggaga gaaaaaatca tgatgaattc agtgaattta
    12421 gtaggacggt taacaaaaga agtggattta aaatacacac caaaaggcaa tgcgacaggc
    12481 acatttattt tagctgttaa tcgaaattat acgaatgcaa acggcgagcg tgaagcagat
    12541 tatatccgct gtgtcatttg gcggaaagct gcggaaactt tagcaaaata tactggaaaa
    12601 ggaagtttga ttggcattaa tggacgtctt caaacgagga gctatcagaa tcagcaagga
    12661 cagaccatgt atgttacgga agttctagtg acagatttct atttgttaga gagtaaagaa
    12721 atcaatgagc aacggtcaaa atcaatggcg aataatcaaa ataattcttc aattccagga
    12781 aatgagattc aaatttcaga tagttatttg cccttttaag tgaaaaataa ttttgttcta
    12841 tacctgattt cttcaggtat agaacaaaaa ataaatagag atgaggcgat tttttatgaa
    12901 aatcattgac agttacgatc aacgaaccat gcaatatttg ggctcgtttg attatacaga
    12961 tgaaaatatt attaattatg ttgcaagttt acctccttat caaagcgttc gcttggtaga
    13021 ttttgcaagt gatgaattgg tattaacaac gatgggcaac tttttagatt atgtcggaga
    13081 caaaaaatgg ttggaaaaaa ttttgtctaa attgattgcg aaacaaacag gagaagaacc
    13141 cataaatgcg attcaattgt tcaatcgata tgaacaagaa aacaatgatt aaagaggaga
    13201 gaaaaaatga aaagattaaa acgaagtatt tttattgtgc tagcattgat tgattttgtt
    13261 gggacaggtt gtgaagcaat tgacgttcaa aaagctccct tatcggaaat aaaacaagca
    13321 ataacaaaac aagatgaaga acaaccgcta aatacacaag tagaaatagt gaatcaaaat
    13381 aaaccgagtt tttctgaaga agatttttcg ttagaaaatg gaaccaggca atttttttca
    13441 gatttggatc gtttaaatcg ggtaggtgta gcaagtgcac ttttgcataa aagcaggatg
    13501 ccaacagaag aacgagaccg attatacatt aagccaactg gctggaaaca aaagaaaatg
    13561 aagaatggcg attatcttta taatcgttgt catttgattg gctatcaacg tgcgacaagt
    13621 ttccccataa gtatgtaata cgaaacggga ggattaatct taagattaat cacaactaac
    13681 ctatttgaca gttggaatga aagctatcat gtctgctgaa tcaactggtc tgatactcct
    13741 acatgcaata aagtctacta ggaagttatt gtatgaagct aggtaaaggc ggcagaacaa
    13801 tttctaagtg tggaatcata tgaagttgga tatgccggga cgctataaat tactcagggt
    13861 gagactgttt ttaattaaac tgacgaactt gcgaatgtac gggtctataa ccgacttata
    13921 ttcgaaagat ataagattgc gaatgcaaag aacattatcg aaaagtaaga atgttggtca
    13981 tgaaattcga gatggaagac tagcggtgta tctgtcaaga gttggtaaaa catgtttgat
    14041 tgattcagat gagagtggga atgcgacaaa acgttttaca gaagaaatag agcaactagc
    14101 agaactgaat atacgatatt tttaaatact ttagcggaga ataaaaaaat cctctacaga
    14161 aaactacgga gggcaacatg tgctattgtt tcaacaatac atttttaatt tttaccataa
    14221 caaaaaagta cttgaaaaaa aacagaaata tgttaaatta aaaatgaaaa aatggaagcg
    14281 gttacttatt cttttgaatc taagataaaa attcaaaaga ataacataac ccaaatatga
    14341 ttatacttga gattctaaat atttatcgaa aaggaatttg ttttaaggaa aaatgattct
    14401 atggacggtt attgatttat atttaccaaa tggctaataa aatagtattt cttatattgt
    14461 atagtttttg tttttatgtg aaaacatgag caatagaatt gtactagtag gataaggatg
    14521 gatggaaaat gaaaaaaact atttttgttt tagcgatttt tatatgtttt gggctacttt
    14581 tggtaggcta cttgttgttt aattctaatg aaatgagaac tatttctacg aatgacttgt
    14641 taaaaaaaag tgaattaaag gataaaaaaa aagaatctct tgcctttata atgtttactc
    14701 aagaagagtg tgagtattgt atagcaataa atccaatcat agatagtgta aataaggaaa
    14761 ctttgaataa gataaagaaa tatgatatat ctgtagattt tcaatctaaa caaaagtgta
    14821 aaattaagtt tgttccgact attgttgcat atagtaagaa taaagaagtg gggagaattg
    14881 acaatgaatt agataatttg tatcgaaaaa agcaagatgg ttcaattgta atagacaaga
    14941 aaaaaacaaa gacattgctt atagattgga tgaaaaaaat ggataaacaa ggagaatatt
    15001 tgtatggaga agattaaaca gacatttccg ttaattaata aatattggaa taagaaaaaa
    15061 atttttatat tttttattac gtttctgctg tcatctttat tttctctaat tttcccgtat
    15121 ctatttagta atgttattga tttatattta gagaatgagt tatcacagaa tattgtgcga
    15181 atagtaacga ttcttatatt tcagttattt ttagtcttaa ttaaagaagt gtatggatat
    15241 ataaaaacta tttatgcatt tgaagttgat ttatcaatga aaattgattt tcttaaggtt
    15301 ttacaaaaaa aaaatctttt tttttcgcaa acgaagcatt caggagaaat tcaatttagg
    15361 atgttttcgg atatttctac tatcgtaaat actatcttta ttttttctgt tgaattacct
    15421 gtgataggcg ttttagttct tgtagcggta tttattataa gtaaagtgaa tcttattttt
    15481 ctgatttttt cactaataat gatgggagct atggtgttac agtataattt atttcaatta
    15541 ccgcttaaga aaacaatcga gaatacaaaa agtgttaatc aaagtattac aggagaattg
    15601 aatgcacatt ttgatcaatt agaaattata agcgcatata attttgagaa tatctctatt
    15661 ttaaaattcg attctagatt taaagaatta tttaatgttt tcaaaaaaca aaataaatta
    15721 ctaacactgg gaagctgttt ggcacaggtt attttccaat tatggattgg aggaatattt
    15781 cttattgatg ttattttatt agctaaagga acaataagta ttactaattt tgtaatagta
    15841 tttatgtacg ctaacatggt ccctacttta tggactactt taattagtat tatttggaaa
    15901 tggcaagact attcagtgtc attaaataga tattacgaat attattcaaa taacaacaaa
    15961 gtggctggca caaaaaaaat aaataaaata aattctatta ctggtcaatc attaaatttt
    16021 tcatataaca aaagaaaggt attaagcaat ataagttttt caactgaaat gaataaacta
    16081 ttcatgatat caggagaaaa tggaacaggg aaaacaacac tgatgaaatt atttactaaa
    16141 cagttgccgg ttagtaataa tcaattattc gtcaacggga tagatattaa tatgattgat
    16201 aacgaatcat taagaaggtt aatttcaatc gcaacacaaa taccatatat aattaatagc
    16261 tccgtgaagg agaatatttt aatggggact aaaaactatg ataaaaaaaa attcagtaaa
    16321 ttaatagaac tattgggaat aagtaaattt atgacgaaat taggtattac tttggacgac
    16381 aatatttatg atgaaaaaat attattatct ggaggagaaa aacaaaaaat aagtttagct
    16441 agggcgctta ttaaatcagc ttcagttatt atattagatg aaccatttag aaatttagat
    16501 aatgaaactc gcgataattt gagggattat atactaaata caaaaaaaga agtttgttat
    16561 ataattataa ctcatgatca cattctagat aattttttgg attcaaatga tgaaatatta
    16621 tatttaaata aggagtatga taataatgaa tagcaaaaaa cttcagatgg gtgaaattgt
    16681 tagaggtcct agtcaaatat ctattgatgt tacatataaa tgtaatttta gatgtaaaca
    16741 ttgttacaat gcgagtggtg gagacaattg ttttatggat aatgagttga ctgatgatga
    16801 actgttagaa cttacaaaag atatatgtaa tttaaagcca tataatattt gtttctgtgg
    16861 aggagaaact ttactgagaa aaaaaatagt tttatcttgt gccactttat taaaagaggc
    16921 aggtattcct aatattgcta ttgttactaa tggatcatta gtaactcagg atgttgcaaa
    16981 agaattattc aaagcaggta ttaatcgatt tcaagtaagt ttagatggaa aaaatgctag
    17041 tagccatgaa gcacttagat tatatccaaa cagttttgaa aaggcaatta atgctatcaa
    17101 attatttaga gaattgtccg aagaagtgga tgttgctttt tgtcctacta gttttaatta
    17161 caatcaatta cgagaagtat atcaactagt aaatactctt aaagtgaagt ctttaagaat
    17221 tcaaccattt atgcttagtg gtaggggaat gcaaaatagg gaaatagtgc caactgtttc
    17281 tcaatataga aaactagttt ctgatattaa ctatattaaa agaaatgaag attataccaa
    17341 gatagattgg ggagatccaa ttgatcatat catccgacac agaactttat tgaaagattt
    17401 ttcgaatttt gtttctatta ggtcagacgg aggattaatg gcatcacctt acttgccaat
    17461 tcaagttgga aatattaaat gtcacagtct ttctaaatat tggcgagcag gactttataa
    17521 agttttttct ttagatttag ttcaagatat agctactcaa gtttcttcaa tacaagatat
    17581 gagtaaagaa ttaaaaaaag gacatattaa atggcttaat gaagatatag aaattgatat
    17641 tctcgatgaa tacaaacatg atattttaat ttaggtttat cattggaaag tttagataaa
    17701 caaagtactt ttagaaagga gggaaaaaaa tgatttctga acctcaaatg aataatatgt
    17761 atgaaagccc aactataatg ggagctggtg aagctgcgcc aagagcttgt tcagcaatta
    17821 tggtgtgtgt gttattagca tctgcaggag taggtatcca agcaggagcg gtgtatactt
    17881 atgctgtagt tgctacagta gctgtagctg cagccgcagt ttggaaaaca gtaggattag
    17941 ttggttagga attaatagga gactaactgt ctcctattga ttccaatgat taagctaagg
    18001 aggaatttta agaaaataat gaaatacaat aatttaaaac cgaaattact taatgagaac
    18061 atagaatata taagagaaga aaattatgga tatttaacca tagttcccaa aaatggaact
    18121 gaattgaaag ataacgtttt caatccaaca gtaagatata tattggctaa ctgtactgga
    18181 aaaaacagtg taaaaaaaat agcgaatggt ttaaaaaaga agtttcctag tttagaatat
    18241 gaaaaattct atcaagatgt tgtaggaact attaaatttt tcaaacacta taatattatt
    18301 gatataggag attatagtat ggaagaaaag aaaatatata aaattgaaca aaatttagaa
    18361 ttagaattac taaatgaatc acattatgaa gaaatttata attttatcac agatcatcct
    18421 gaaagtatgt attttcatga agatatttat catcaaggaa atagtaaaga attagaagtt
    18481 gtccttagat ataatttatt ttcgtttacg agtgaatttt actttttaag acataaagag
    18541 agaattgtag gaatggtttc tataattgtt aatccatttc aaaacttatc ctacagtata
    18601 gtaaattacc tcatatgtga gcctgcatat actacgacta ttttgacaac agtattagaa
    18661 aatattaacg aagtttcaat tttagaagta aagaagctag tgattcattt gctaaattca
    18721 acagaagatg aatcactaat cccctattta aaagattcac atttttttaa agaagcggta
    18781 ctatctaaag aatatagaga gcatgatgtt ttgagatacg cttacattta ttaaataaaa
    18841 tttctgatag tattttaata attcggatca tatcataaga tataggaggg aaaagaagtg
    18901 attttattga ttttgtctct cgtattacta gttgtatcta atctaatgaa aaataatcct
    18961 cctacaaaaa ttaattcttt ttatggcttt agaactaaaa agtcaatgaa aaatgaaaaa
    19021 agttggcatt tagcacaaaa attgttgggg gatttttcaa aaaaaaaagt tatcccagta
    19081 tttttgatgg gtattgtttt tttcagtata gagatttata gtttagctat tttaaaaaat
    19141 gaaaattttt tcgttagtgt attaatgggt gaaagtttaa tcctaatact gattttgtgt
    19201 ttaattgttc atgaagtgaa taaacaattg tgaattaaaa aaataaaaac ctagtataat
    19261 cactttaata aattgttggt agaaggtgat caaaatggga gtaaacgaaa tgatgaaagt
    19321 cagattgagt ttttaaatga taccttcctc agaacttcaa aacgaattca aagattcgtt
    19381 gacaaatctt gagcaagatc atttaaaaaa atattttctc ttcatttgac gaaaaatgtt
    19441 tagaaatctt atataagtga agataaagta attggtgtaa ttaattgtag agaatagcct
    19501 ttgagcaatt gaagcgatac aattatttag aaaatgaact tcctccagca tactacatga
    19561 ttccttacgg gacgatgtta aagaagaaag caagaaattt tagaagtatt agtagataaa
    19621 ctaatgggct tttctcaatt taagggagta gcccaaatgt ttatcaaata tggtgcagat
    19681 acagtagcaa ccatgtttac agaagaatat caagtagact attatgaagt aatagataaa
    19741 tgtgtacatt ttgtttatga aaaagtgtgg aattttatga attttgcgat aagtgaagaa
    19801 atcttacgag tacaagaaaa cagaacagga atatttacca gagacagaaa gcttaatttg
    19861 attgcgcatt atatacggaa tcgctggtca tcaataccaa attttaatta taaaaaataa
    19921 ttatgaacaa tcttttatac aatatacaca ataaatcaag tagtatttat gaaaaaagac
    19981 ttcaaaaaca gaatgatttt ctatcagtct tagaaattat agagctaaaa attttggtca
    20041 cgcgtagaaa cgcgtttttt ttgttttttt gatagtaaag aacgtacaaa acaatgagaa
    20101 tttaaaagat tgtactacca gtttttttac cttttcattt gagtttagag taagtaaaat
    20161 attactataa aaacacatat tcttgaagaa atcttttttt tgctgtattc atttagatta
    20221 tgatagagaa aatacgtgag agggggacct gggatgtggc tatctgatga aaaaattcag
    20281 tggcgtagct atcagaagcg tatattgact aactttgcta cttatttaaa taacaattca
    20341 gtccatctag tggctccacc aggggctggc aagacctttt taggaattga gatgatccga
    20401 cgtttggatt gtaaaacctt aattttggtt ccttccctaa tattgaagaa acagtggtta
    20461 acaacaatcg aggaatattt cttagtaaat ccagcagata gttgctatct ttctggggcg
    20521 attgaacatc ctagtcatat tacagtggaa acgtatcaga ccatttacaa ccgactgaag
    20581 gaggagccaa cttatggtga tcagctggag ctgttagttt tttgatgaag ctcatcattt
    20641 aaaaaaaagc tggggtgaat tgttgattga cttgaagaaa aaacaaccga atctgttgac
    20701 gatttccttg acagccacgc ctccgattga tagctctcag acggagtgga agcaatatat
    20761 tgagttaaaa tggcctgatt gacgaagaaa ttagcattcc cgagttggta aaggaggagg
    20821 ttctatgtcc tcatcaggat tttctatatt tgattccggc taatcaggaa actactcagc
    20881 attattttac ctttttagag aaacaaaatg agctggtgga agcgttaatg gaaagccaag
    20941 agacggtgga ttatttgctt tctttagaat ttgttcagca tccgcttgag cattcgaagg
    21001 aaatttatga aaattttgag ctgttcacta gtgcattaat ttacttgaat catcataact
    21061 atgaattgat acccgaacat tggcagctat taggcagttc taagcagcaa ggtaaaaaag
    21121 cttcagttcc atctttaggt aaaaaagagc tgatcactat ttatcagcag atgtttgaag
    21181 atcaaccgga attgcctatt ttttatcaat tgaaaaaagc tggatgattg tttgatgagt
    21241 cgctgcattt gtttccagag tatccagaca ctgttggtag tcaggcgatt cctgagaaaa
    21301 agcaagcagt ttgtcaaatt ttgttaaaag aagaagttca gctgaagcaa gaactgacag
    21361 cggtcatttt gatggatcgt atttgcaagg aggtattagc aggcagtgat ggtaccttag
    21421 cttatggcgt ggttcctttg tttgatgaag ttgttcgtct attaggtaac actcagcaaa
    21481 tcggtgccat ctgtggagag ttcttactgt taccgaaaat gctagtaact gttgagctgg
    21541 cagaactagc gacgggtatg aatgaacatc cttatatgtc gggttatttt tatttgacgg
    21601 tggatgacaa aaaccgccaa gcattgatgc aagcagttac tcgcctatta accgaaaaga
    21661 agttgaatat tttggtagga accgttagtt tgctaggaga aggctgggat tgtcctgcaa
    21721 ttaatctgtt gatcttggct aatcaagctt ccacttttgt gcaaacacaa caactacgag
    21781 ggcggggctt acgcctaggt gcacaaaata aagtaacgaa catttggcat ttagcgattg
    21841 gattaccggg agttcctctg tctgaacagc ctgatttgaa aagaatgcta cggcgtttgg
    21901 gcaatatttc aggtctgacc tttgaatctt caccaaaaat tgagagtggt ggcgatcgct
    21961 ttgctttacc actggaatat acgaccacca cgatggaaac ttatactcag tcgatgttct
    22021 atttttcttg tgaacgaagt cggttgaaag aacaatggag tactgcttta gctgatggct
    22081 ggcacctatc tatgccaatc gtcgttagga aagtcgatca ggtaaagcaa aaacaagaaa
    22141 cttcattgcc gatagtagta aaaaatatag agcctctaag cttctggtca gctatattta
    22201 aaggtaactt gctggaatat ttccaaattc gtaggcgaca aaagctatgg gaaaaggaat
    22261 gtgcccgcaa aaaaagcttg gccatatgcg ttcaaacgac gttgcagcgc cttggtgaag
    22321 ttagtgatag aaaagtaatc gtagcggctg atcagcagga atttagatgc tatcttgaga
    22381 atgctggcta tcgagaagga cagcttttta ctgaagctat gagggagctt ttagcaccgc
    22441 tggagaatcc gcggtattta atcgaaggga aaaagatagt tctgacggta ccagaacgct
    22501 ttggccgaaa taagcaaatc gctaaccttt ttctaaacga attaaatcaa tcgtcgctgc
    22561 ccaaatgtca gttggtgtat actagaaatc ctgaaggacg ccaaaagcta gtgacttatc
    22621 gaatgaggca agtcttccag acccgcgatg aagtaattat tcaaaaaaac atttggacgt
    22681 aagttgatga aaataaattt tgctagagta gaacaagtcc gagaattaag ttgctactta
    22741 actgataatg ccaaggacag gagaatcttt tcttgacgac tgaaagaaac tatttgactt
    22801 atataacggt gtgtgatgaa tatttgcaag gcattctctt aaaaagtact cgtaatagtt
    22861 tttttgaaaa caaaacagta ctttattaat aggactgaca gagcgatata agaacatctg
    22921 cagacagagg tgaatgcttt tctcatgatc gatgaaacaa tggtacttcg gatattcttg
    22981 cttttagttt gaaccgttgt gattttcttt gattcctaaa agtagtttta tagaatgaaa
    23041 atcaagcata cggaatgctc ctttgattgt gttgtggtga ttcaatctaa tggagcttct
    23101 atatgttttt ttatttgcaa aaagaaccga tagaatattt ttatcagtct tagaaattat
    23161 agagccaaga aatttcaagc acacataaaa acgcgtgttt tatgcatttt atgagcaaaa
    23221 ataactaaag accattacgt aaaacatagg taaatcagca ttcaatctca aattatgtga
    23281 tcaaatgttg atattatttg attataagtt ttcttttgct attatcagtc tgcaaataaa
    23341 cagattatag agtatgaatg tagatagttc aatctttgtt tacgacctca catgtttttt
    23401 tactcaagag aaagtaatat aaagtgacta tatttgacag aactttacat aaaaaaagta
    23461 gttagttttc tgtagtaatc agaaatgtaa ataaattaaa aaaaagaaat ttagcagtta
    23521 tatattttta aaatattttg ggtgagtagt caatacaaca ttttgttcat caagcattaa
    23581 atgaagatgc aaggatgtta agaagttagc ttcttcaggg tcttgcatgg tcaaggcttt
    23641 aaatagttga tgatgttgtt ccataatttt ttcccatttt aatccttccg tttttactcg
    23701 taaccaacga aatcgttcca agtgcgtatt attatcttcc aaccattgcc atacttcttc
    23761 ccgttctgca atttcaaaac aagctttatg aaataaatta tcattatgaa aaaaactacg
    23821 agcattcttt tgactaacag ctttttcttg ttctgcaatt atagtttcta aaacttgttt
    23881 cttttgatta ttcatcttgg cacaacactc tagcataatt tgtcgttcta aatgttctct
    23941 gacaaaacga gcattttttg ctaaatctaa gtcgattaat gagatataat ttccactttg
    24001 aggaattgta tcaacaagtc cttgatgacg gagttgaatc aatgcttcac gtattggagt
    24061 acgaccgata gaaagcgttt cttccaattt tttttctgat actttttttc caggttctaa
    24121 gtcagcgtag ataatttgct gacgaataga ttgataagct tgattttgga gactagtttt
    24181 aattgtcata tttaatccct ctcaaaattt catttttgaa tttgatattt tagttctatg
    24241 ctcgttatat aatatcaaat ttcaaagtaa catactagct attaaaaaaa caaaaaatac
    24301 tcaaataaga tcaaagagaa attaagcttt aatttaagtt agttctaaaa aatatatatt
    24361 ggtgtgcttt tactaaaaaa agacacaata attctgacat ggtagaaaaa agaatatttt
    24421 tagttttttc ttattgacag cgattacatt cgattttata ctacaattgt aacggaaact
    24481 aacatatcag tatggagggg taaatatggt tttgattaaa gactggcaag attctatcga
    24541 aggattgaac aaaatgcaag ttcaactacc gtattttaat gtcgaagcgg tcaaacaaga
    24601 aggtcagaaa catccagtgt ggcttcattt tggtggagga aatctttatc gaggatttca
    24661 tgcagaaatt gctcaatgct tagcagatca aaatgaatta gaatccggag tagttgtgtg
    24721 tgagacattt gatgaagaag tagtagaaaa agcttatcat gactacaaca atgatatttt
    24781 ggagattgtt atgggtgaag atggtgaact caaaaaaaga cttttagcag taacagctga
    24841 tagtttatat tgcaatccaa agaatataac ggactatcaa agagtggtta aatattttga
    24901 agatcctaca ttacagttta caacgtttac tattactgaa aaaggataca gtttaaaaga
    24961 ttctaaagga caattttttt cggtagtgga agcagatata aaatctggac ctaccaatcc
    25021 tcaacatacg atgagtattg tgacatcctt gttgtatagc aggtttaatg ctggagagtt
    25081 accgattgcg atggtttcta ctgataattt ctcacagaat ggtcaacgtt ttcaagatag
    25141 catttttatg attgcagaag cttggcaaaa aaaaggatac gtttctgaaa aatttattgc
    25201 ttatttaaag gataaagaaa aagtgacttt tccttggagt atgattgata gaattacgcc
    25261 aaatccttcg gaaagtgtca aaatggattt attgaaggcg ggtttcgaag acttagatat
    25321 aattcatact gcgaaaaata ccaatatcgc tgcttttacc aatactgaaa aagtccatta
    25381 tctagtcatt gaagatagtt ttccaaatgg tcgacctaaa ctggaagcgg ctggtgtgat
    25441 tatgacagat agagaaacag ttgataaagc agatatcatg aaggttacga catgtttaaa
    25501 tccgctccat acggcattag caataacagg ttgtttgttg gggtataaat caattcaggc
    25561 agaaatgcaa gatgatgatt tggttggctt aattaaaggg ataggttatg tagaaggctt
    25621 accagtagtt gaaagtccag gcgttattga tccaaaacaa tttattgatg aagtaatcaa
    25681 taaacgtttg cctaatccct atattccaga tacaccgcaa cgaattgcaa cagatacttc
    25741 tcaaaaagta gcgattcgct atggtgaaac aattaaaaag tatgtcgaaa aagacggaaa
    25801 tgcagaaaat ctaacattta ttcctatcgc tattgctgct tggttacgtt atttattggc
    25861 agtcgatgat caaggaaaaa attttgtgcc aagtcctgat cctttattgg tagagttgca
    25921 ggaaaaattc tcagtattga aattaggtga acaagatcct gcaaagattc attgtgcggt
    25981 acaggaaatc ttagaaaata aggaaatatt tggtttagat ttatatcaag ttgagatagg
    26041 aaagaaaatt gaaaaaatct tttgcgaaat gctagtaggt cctcaagcag ttcgtaaaac
    26101 aatcaataaa tatataacag aagttggagg aaagtaacta tggaaatgac ttttagatgg
    26161 tatggggcaa atgaagaaaa aatctcacta caagaaattc gtcagatccc agcaatgaaa
    26221 ggaattgttg gaacattaat ggatatacca gccggagagg tctggccgag agaacgaatt
    26281 aaagctttaa aagatgaagt cgaagccaat ggccttacct tgaaagttat tgagagtgtt
    26341 aatgttcatg atgatatcaa aattggtttg ccaacacgag atcaatacat cgagaattat
    26401 aaacagacga ttcgcaattt ggcagaattt gggattgagg ttatctgtta taattttatg
    26461 ccagtctttg attgggtaaa atcagatttg gattatcatt tagatgatgg gtcttctacg
    26521 ttagcctata tttcaaaaaa tattcctact gatccagagg aaattattag aaaagtagaa
    26581 aattctacta ataattttga attgccgggt tgggaacctc atcggttatc acaaatcaag
    26641 caattattag aagcctataa agaagttgat gaagcgaaat tgcgagagaa ctttgcatat
    26701 tttctcaaag caattatccc gacatgtgaa gaagttggga ttaaaatggc agttcatcca
    26761 gatgatccac catattctat tttcggatta cctcgtattg ttaaaaatcg tgacgattta
    26821 gaatggttgt gtaatgtagt ggattctcct tctaatggta ttacgttatg tactggaagt
    26881 attgcagaac gaccagataa tgatgtctat gaaattctat ctgaattttg tcaacgggat
    26941 cggattcctt ttgcacatgt tcgtaatatc aaatatttaa gtgataatgg actcgatttt
    27001 tatgaagcgc cgcataaatc agaatatggt tctctagata tgtataagat attaaaaaca
    27061 atgtacgata atggatttaa tggctatatt cgtccagatc atggtcggat gatttgggga
    27121 gaaaccggta gacctggtta tgggttatat gaccgagctc taggtgcagt ttatttaaat
    27181 ggtatttggg aagcgcttga aaagggggcg agtaaataat tatataatgg agtctattta
    27241 gaagaaaaat tttctcttct aaaaactaag ggaaggaaag gagtgcttaa atatgttttt
    27301 agataagaac tttttattag agaatgaaat agctaaaaga ctttttcata aatttgcaga
    27361 acaccaacca atcatcgatt atcattgtca tttagaccca gcatgtttgc tagaaaataa
    27421 gcaatacaag aatttgactc aggtatggtt aaatgataaa ggagctggag atcattataa
    27481 atggcgattg atgcgagcta atgggataga agaagagttg atcagtggtg caggtaatga
    27541 ttatgaaaaa tttttagctt ttgtaaaaac agtggagaag gcgccaggta atcctatttt
    27601 tgaatggtct catttggaat tacaacgtta ctttgatatt gatttgacaa tttctgagaa
    27661 gaatgcacca gaaatttggc gacgtgccaa cgaaaaaatc caaacggatt cctatcgccc
    27721 aaaagaattg ctgaagatga tggatgtgaa agccatttgt acaacggatg atcctacaga
    27781 tgacttgcga tatcatactg agctttctaa acaggataat tctttcaagg tattacctac
    27841 tttccgtcca gataaggtct ggagtatcaa atcatcagga ttttcgaact atattttaaa
    27901 acttagtcaa gcagcgggta taccaatcaa aaactttaat gggttgaagc aggcattgag
    27961 ccaacgagtg gattattttc atcaaattgg ctgtcgatta gcagatcacg gtttgaatac
    28021 ttatttttat gaaatagcag atgaaaaaat actcgacaat ctgttaataa aagctttagc
    28081 aggaaaaacg gatttccatt tatcagaaat tgcacaattt gttacaggta ttcaattaca
    28141 tttgatgtct gagtatacaa gatatggttg gacgatgcaa atgcatttaa atgctttaag
    28201 aaataattca agtatattaa aggaacaaat tggagtagat gtcggtggtg attcgatggg
    28261 ggatcaagct agtttgacac aaaatgttgt agcattgttc gacactgctg aaaagagtgg
    28321 gtgcttacca aaaacaattt tatattcatt aaatccaacc gattggcttc cactagcaac
    28381 agcaatgcaa agcttccaag ggggtatgat tcaaaaaatc caattgggat gtgcttggtg
    28441 gttcaatgat acagctgaag gaatgcgtca acagttgact acgatggcgc aacaaagttt
    28501 gttgtcgaat tttacaggta tgttgactga ttctcgaagt tttctaagct atcctcgcca
    28561 tgagtatttc cgtcgagtat tatgtcaatt actaagcgag tgggttaaag caggtcgaat
    28621 tccagatgat gaaatgtatc ttagtcagat agtggagaat atttgttatc gtaatgcgta
    28681 taattatttt aatttttttg aaatcgaaag tagaaaataa tacaaaaaat aaaagcttaa
    28741 atttatttat tgtaagttag tacaactagc tagagtatgt atccataaaa gaaggagata
    28801 gatatgaaaa aagtagaaat tttaagtaag ttagaagcgg ctggagtcat tgcggttgta
    28861 cgtggaaaaa ataaagatga ggccattaaa gcaataaatg caattactgc gggtggaatt
    28921 aaagggattg aactaacttt tacagttccg aatgctgaag aaataatcaa agaaattgct
    28981 aaaaaatatg ctgatcaaaa agatgtaata attggagccg gtacagtttt agatgcgttt
    29041 actgctcgta ttgcaattat ggctggagca gaatatatcg taagtccatc ttttgatcga
    29101 caaacagcag agatttgtaa tttatatcaa attccttatt taccaggttg tatgacaatt
    29161 actgagataa aggaggcact taccgctgga gtagaaatca ttaaactatt tccaggttct
    29221 gcttttggtc catctgttgt aaaaacgtat caagctccat taccatatgt aaatataatg
    29281 cctactggcg gagtaagtct agataactta gaagagtggt ttgaagcagg cgttgttgca
    29341 gttggtgtag gtgggaattt gttggctcct gttgaaaaag gtgattttga agaagtaact
    29401 cgaatagcta gtaaatatat agataagttt tatgagataa agaattatta ggagaaaaaa
    29461 taaataatac tataaaatgt aaaaaataaa tactaactct ttatacttca gagtatagta
    29521 cgtaagttgg cagttcaaca gaatgaaaac gcttaacaat aggaaggagc gcaaattata
    29581 tggaaagaaa atttggtcta agagacaaaa taggatatat gtttggagat tttgggaatg
    29641 atttcacttt catttttgct agttcttttt taatgatttt ttatacaaaa gtattgggga
    29701 taagcggagc taccgtagga acattatttt tagtagcaag atttattgat gcagttacag
    29761 acataacaat ggggcgtatt gtggatggat caaaaccatc taaaactggc aaatttaaac
    29821 cgtggatttt acggatgagt ggtccagtcg ctttagcaag ttttttaatg tatcaaacat
    29881 caatggcatc agcttctatg agtattaaag ttatatatat gtatgtaaca tatatactat
    29941 ggggaagtat tttttataca tctattaata ttccttacgg ttcaatggca tcagctatca
    30001 cttctgttgc agaagaaagg acagcgttat caacatttcg tagtatagga gctaccttag
    30061 caggtatgtt cattggcgtg ttaacaccaa tttttattta tacaagagat agttctggag
    30121 ctcaaattgt acgtggtgga tcaactttta caattgtggc aggagtattt tcagtgtgtg
    30181 cagttatttg ttacgtaata tgttataaat taactgtaga acgggttaaa agtgatgaat
    30241 cgcagttaaa agtttcattg ttgaaatctt taaaaggatt agctaaaaat agagcgttgc
    30301 tttctataat agctgcagct atttttttgt tgcttgctca gcttttaaca atgagtatga
    30361 acaattatgt atttccagat tattataata atgcacaggg aatagtttta atgaacttta
    30421 tcaatcctat tttgatgttg gtacttgttt cacctgtaag tttaattttc agtaaaaaat
    30481 acggtaaaaa agaattggcg tcagtaggga tgttcttctc tgctattgta tatttacttt
    30541 tatttttgat aaagcctagt aatatgtatg tattcttagt tcttacttct gttggatata
    30601 tggggttagg tatttttaat acagtcattt gggcgaatat tactgatgta atcgatgatc
    30661 aagaagtgaa atctggccaa agagaagatg gaactattta tgcagtatat tcttttgccc
    30721 gaaaaattgg acaagcttta gctggcggtg tcggaggttg gactctttct ataatagggt
    30781 atgattcgtt agcaaaagta caaagtggag aagttttaca aaagctgtat aactcttcaa
    30841 cgttgattcc agcagtttgt ttctttgttg taggattgat tttatttttc gtatatccat
    30901 tatctaaaga aaaagtatta gaaaattata atttgctaaa tcaaaaacat aactaggaga
    30961 aaaaacatga acagttggat attttgtatt gattcagatg gttgtgtcat ggatacaatg
    31021 acttataaac acaatctctt tttcgggcca attgctgcag agatttttga tgttcatcat
    31081 agggaaaaat tcctacagga ttggaataat attaatttat attcagagac tcgtggaata
    31141 aacagatttt taggtttagt taaaacatta gaaaaaaatg atgtaccatc gattaatcga
    31201 ttaaagcact gggtatctgt aacaaaagaa ttgtctaatc catctttgaa gagggaaata
    31261 gaatactctc agtcagagga tttattaaaa gctttaaaat ggtcggaaga agtcaatcga
    31321 cagattagtc aagctaaagg attagataaa ccctttttaa gtgctagaga tactataaaa
    31381 gctctgaaaa aatatgggaa gataattatt gtaagctcag ctaataagga agctattcaa
    31441 gaagaatggg aaagacatga attattatcc ttagttgatg aactgtgttg tcaagataaa
    31501 ggaaaaaaag aagacattat tcgaagtgta attgaaaatg gttgtgattt ggataagata
    31561 ttaatgattg gtgattctcc tggtgatcta gaagctgcaa ataagaataa agttttcttt
    31621 tatcctattt tggttaataa agaaaaagaa tcatgggaaa acttaagaac tgatatttta
    31681 tttaaatgtt tatcgggaga gtatatagat attgaacaaa aatatataga tactttttgg
    31741 aaaaatctta ctaataagta gaggagaaag aaaatgacta taaaagtaga cttacgaaaa
    31801 aaaccatatc atttagatga agatgctatt tcttggattg aagagacgat tgatgctatg
    31861 actttagaag aaaaaatagg tcaattattt gttaatatgg gatctagtcg tgataaaaat
    31921 tacttaagta gtatggtcaa tcagtaccat attggagctg tacgatataa tccaggaact
    31981 gctgatgagg tatatgatca aaactatatc ctacaaacag aaagcaaaat tcctttatta
    32041 attgctgcta atacagaagc aggtggtaac ggagcttgta cagatggtac cgaagttggt
    32101 gtagaagtaa aaattggtgc aacaaatgat gcgaaatatg cttatgaatt agggagagtt
    32161 tctggaattg aagcatcggc tattggatgt aactggagtt ttgctcctat tgtagatatt
    32221 aatagaaatt ggagaaatcc tatcatttct agtcgatctt ttggacaaag ccctgataaa
    32281 gttttagaac ttagtttagc ttatatgaaa ggtattcaag agagtggaat tttgcctgca
    32341 gctaaacatt tccctggtga cggtattgac gaacgagatc aacatctatc ttttagtatt
    32401 aatagtttaa gtacagaaga atgggataat tcttttggaa aagtatactc aggcttaata
    32461 gaagctggat taccttcaat tatggcaggt catatccata tgccagctta ttcaaaacat
    32521 tttaatcttg atctgaaaga tgaagattta cttcctgcaa ctttatcaaa agaattaatc
    32581 actgacttac ttagaggaaa actcgggttt aatggattag tagttactga tgctagtcat
    32641 atgttaggaa tgactggggc aatgaaacgg tcagaattat tgcccacttc tattgcggct
    32701 ggatgtgatc tattcctatt ttttaatgat ccagatgaag actttaatta tatgatggaa
    32761 ggttattaca aaggaattat ttcagaagag cgtttacgtg atgcattaag aagaatttta
    32821 ggcttaaagg ctaaactagg tttgcataaa aaagaaaaga ataaaatttt gttaccgaaa
    32881 caagaagcta tggaaaaaat tggtttaatt gaaaataaat caatttttaa agaggtagct
    32941 gatcaagcaa ttacactagt taaagataaa caagatattt ggcctattgt accagaaaaa
    33001 tataaaagga ttttactggt agatgtacat ggggtaaaag gtgggtttgg tgaagtagtt
    33061 gctggaaata gacctcaggc cattgattta ttgaaacaga ttttgtcagc aaaaggattt
    33121 gaagttgaaa aatacatttc acctgaagaa gaattgaaag gattatcgca agaagaaaaa
    33181 attaaaaata tagctggaat atatgctgct aagagaccaa tcaaagattt aactgaaaaa
    33241 tatgacttga ttattaatat agctaatgtt aacccaggta cagttcaaag aattgtttgg
    33301 ccagcaagta aaggtactcc ggatattcca ttttatattc atgaaattcc aacaattttt
    33361 gtatctgtac agtgtccgtt tcatttagct gatgtgcctc aagtaaaaac ttatatcaat
    33421 acttatgatg gaaaacaaga aactttagaa atattagtgg acaaactaat gggcctttct
    33481 gaatttaagg gaataagtcc aatagatgct tattgtgggt taaaggatac atttatttaa
    33541 tatttaaatt atataggagt gatttacatg ttatatccca ttatgacaga gacgaggcaa
    33601 gtaattgatt taagcggtct ttggagattt aagttagata caaatgatga cttacaagaa
    33661 gaacttgctt tagaaccaat ggttgaaaaa gagagttatc caatttcagt tccttcttct
    33721 tataatgatt tatttgaaac agagaacatt agaaatcatg ttggatgggt gtggtatgaa
    33781 aaagaagtga ttcttcctaa aaaattatta gaagaaagat tagttcttcg ttttggatct
    33841 gcaacacatg aagcaaaagt atttttgaat ggaaaattga ttaatcaaca caaaggaggg
    33901 tttacgccat ttgaatcaga aattaatcag tttataaaag atggaagtaa cagaattaca
    33961 gtggctgtaa acaatgttat tgacgatagc actttacctg tagggatttt gaaagaaacg
    34021 atacaggaag atggagacat aaaaaaagaa aatttggtta attttgactt tttcaattat
    34081 gcaggcatta atagacccgt taaattgtat acaactccga aagactatat aaaggatgtc
    34141 actattaata caaaaataat tgattcagat gcaaatgtag aatttaatat cgatacagaa
    34201 tggaccggag aaattctaat agcaatctat gacgaagaag ctcaacttat agcagaagct
    34261 aaggggaaag aaggagtgat tcaacttaag aacccaacac tttgggagcc tctaaatgct
    34321 tacttatata gtatgaaagt tagtttaatt aacgaaaata aagtgattga tacgtacaca
    34381 gaagaatttg gcattcgtag tattcaggtt tcgaatggga agtttttgat taataataaa
    34441 cctttttact ttaagggatt tgggaagcat gaggacacgt atattaatgg tcgtggtttg
    34501 aatgaagcag taaatatcaa agattttaat ttaatgaaat ggatcggtgc taattcattt
    34561 aggacttcac attatcctta ttcagaagaa attatgcgct tagcagatcg agaaggtatt
    34621 gtagttattg atgaagtacc tgcagtagga ttacatttga atttcatggc tactttatta
    34681 gatgacaacg tagaaaaaca tgatacttgg aaagaaatac ggacagaaaa tgctcacaaa
    34741 atggcgatta aggaattgat agaacgcgac aagaaccatc cgagtgtcgt aatgtggtct
    34801 attgcaaatg aaccagattc ggattctgaa ggcgctaaag aatattttga acctttagtt
    34861 gaattgacta aagaacttga ccctcagaag agacctgtaa caatcgttac ttacttaaag
    34921 tcaacaccag atatttgtaa agtaggagat attgtcgatg ttttatgttt aaatcgctat
    34981 tatggttggt atgtttcagg tggagaccta aaacatgcaa aatatctgtt gaaaaaagag
    35041 ctagatggat ggatggaaag atgtcccgat acgccaataa tgtttacaga atatggagca
    35101 gatacagtag ctggacttca tgatacagta ccaacaatgt ttacagaaga gtatcaagtg
    35161 ggttattacg aagcaaatca cgaagtaata gataaatgtg acaattttgt tggtgaacaa
    35221 gtgtggaatt ttgcagattt tgaaacaagt caagggattt taagagtgca aggaaataag
    35281 aaaggaatct ttactagaga tagaaagcct aaaatgattg ctcactatct aaagaaacga
    35341 tggtcatcaa taccaaattt cagttataaa aaataattta ccttttataa ggaatataaa
    35401 aaaggagtgg ctgcttaatt gccaactcct tttttttata aaaagatttt ctgtttaaag
    35461 aaggatattt ttttctagtt ttttcgaaaa aaaggcgctt tctgattgtt gtattagtgg
    35521 aaaggggaaa aaaggagcat atttatcatg caacaaaatt acaatgagaa atctatacaa
    35581 catcaatttg atgcctactg taaaaaagta ttacgaaacg aaactaagtc tattcgaaaa
    35641 cgaaacatta acataacaga taatgaggaa ccattgacga attcaaatga aggtaagtac
    35701 tcccaaactc attttgatga acaagatgtc tatttcttat ttggaatgga aatattaatt
    35761 tcagatcaaa agttatcggc agccattgat catttatcgg aaataagaag aaaggttatc
    35821 ctactttatt attttgcagg ttttaatgat actgaaatcg gtaaaatcct caatatgagt
    35881 acaagtggta tctggtatca acgaaagaag gcagttgaac aattaaaaat ggggtatgac
    35941 ttatggtaga caaccccaaa aaacggcagt atccattagt tcctctatcc attatccaat
    36001 ctgctctaga agaagatcca atttcaatca agatgatact caaacattat catagttata
    36061 tagctaagtt atgtttgaca aatggattta atgaggcggg gcaatttatt acctatgtag
    36121 atgagtatat gctaagacag ctagaaatta aattgataga agcgatctta aaatttaaaa
    36181 ttaactaact aaaaagtccc ctttccactt attctgttta aaagaatctt ctaatagtta
    36241 aaggattagt agattctttt aaacagaaaa cctattgatc attgaaattt aaatacattc
    36301 tatcagtacg ttaacacttc aaggagcata ttttagttgc gccatgattt cttactgtaa
    36361 agaaggagcg ataaacattc taaaataatt tttatagtca ataaaaagcg atgatttgaa
    36421 gttgggataa tgatacttcc gtaaggctcg ccgtaaactt ggagagaggt gaaattccta
    36481 tgtgcttttg acaaaagcag ctgattaaga attatttcca tatgttgatt tcaacatttt
    36541 ttctattatt tttaagacca aattcagtga ggcaaatagc atcatgtaaa tttagttttt
    36601 ctattgaacg ttctccactt tttagttttt ttatagtact caaatttaat cctgtatgtt
    36661 tagagatctc atttgcagta tggttttgta aagtttctcg gattattgtt atattagcaa
    36721 tttcattacg cataattatg ttcctttctt atcaaaaata tcaatgacta cagtgggctt
    36781 ttagtgaaaa aaaggggtac tacagtctct tttgaaaaca aaaaaataca gtatatacgt
    36841 tttctaaatg ctaagcacca acgtttacac tgtatttaag ggtttggcac ccaatgggct
    36901 gtgaggggct cgaacccgcg acccgatgat taagagtcat ctgctctacc aactgagcta
    36961 acagcccaaa ttaatacttt gttattctaa cacctaaaat aaaaaagtgc aatacttatt
    37021 atcagaattg agagtgaatt ttatggattt tcaagattat gaaaaaatat ttgaaagtta
    37081 tccatcgata ttgaaaatta ctgatttaac taaaatgtta aatattggaa aaaataaagc
    37141 ttataatttg ctgaaagata gatcaataga aaatataaaa attggagcaa ggtacaaaat
    37201 acctaaaaaa gctgtcattg agtatatatt gaaaatacaa gatgaaggta aaaatcatgc
    37261 tataataaaa tagtcaaaat ttttgccgat atttcagttt aagaggagag attgaattga
    37321 aattaagagc aaacataatt gaaaaaagag gaacattaca agtagttgta tcttataaag
    37381 actctaacgg aaaaattaga caaaagtgga aagatacaaa attaaaaaag aaaggaaata
    37441 aaaaacgagc agaaaaaatt agagatgaat ttttactaga attggaaagt caattagcta
    37501 tggaatgtga atgtgctaat ccaaatttat tgttttatga ctatttgctt aattatttgg
    37561 aagtatcaaa aaaacaagta gcatttaaca catatgttgg gtataagcat tacgtccata
    37621 atcgaattta caagtttttt tatcctaaaa aaattaaact aaataggtta aagccttatc
    37681 atttacaaga gttttatcaa tatatgttaa atgataactg tacagcaaat acggttattc
    37741 attatcacgc atttatacgc aaagctcttc aagaggctgt tattacagaa ttgataagta
    37801 caaatgtagc agataaagta agaaagccaa aaaagaaaca atttgtttct caagtttata
    37861 atcaacaaga gatcttgaaa ttacttgaca ttttgtcaga agaaaaactc tatttggtag
    37921 ttcttttaac agcattttat ggattgcgaa gaagtgaagt tttaggatta aaatggtctg
    37981 ctattgattt tttaaacaaa aaaatcatga taaaccatgt aatcatagaa aatccagaga
    38041 atatgtcaca gttaataaag aaagatcaaa caaagaactc ttccagctat cgtacattac
    38101 ctttggttga aacgattgaa cgagcattag taaatcaagc taaatggcaa caagataatc
    38161 gtattctttt aggagatgat tatattttag aagataatga gtacgtattt actatggaag
    38221 atggacggtt gatgaagcct caatatgtaa cgcatcgttt gagtaagttg attaagaaaa
    38281 atggattgaa aaaaattcgc tttcatgact tgcgtcattc taatgcttca ataatgttag
    38341 atagtggcca gaatatgaaa tccattcaag aatggctggg acatgcatcg tattcgacaa
    38401 ctgctaattt atacacgcat ttaaccgcag gagtaaaaga tcgtgcagca gaagcattag
    38461 aaaacgcatt tgggtttaaa gaataaaaaa acaaaaaaca atatctttgg ggtatcgctt
    38521 cttgttttta gaattttttt gttggcaatt tgttggcagt atgttggcaa tgaaaaaatt
    38581 cacaaactaa aaacctattt ataaagcttt ataaaacttt tttcacaaat agagcatttg
    38641 gttaagagtc agctgctcta ccaactgagc taacggcccg tacaaaaagt acttaataat
    38701 agtagcacta agaattttaa aatgcaagac tacatttaaa gaaaatcaga aaaatcttta
    38761 cttccttata ggaaatcatt catagattat tcacatttgc tcgctattat ataagcatac
    38821 caacaaacaa agttcttgca agagaacaaa ccctaacact tttttcattt tttactcctt
    38881 tcccgccgtc tccccggcgg gtctcctgtt cttaacgata aaagacaaga aggcttagcc
    38941 ttcttgtttt ttgttttcat ggaatcccgc taatcaaatc acctagttaa agcaactaga
    39001 ttgaatgttt atcataagtc tgatatcatg tttatgaaat gaaaaaagga gcggttatga
    39061 tggaaaaaat gcgtcagcaa ttaatggagt gggggaagga actagaaaca ttccgtctgc
    39121 ctcactggga agaactccct gacttagaac tttatatgga ccaagtcaga acgttagttg
    39181 atcgttatct ttcacctgtg atacaaggag aaaagcatcc attgcttacc tcttctatgg
    39241 taaacaatta cgttaaacta ggtttgatac cagcaccagt taaaaaaaga tataacaaag
    39301 aacacgtagc atttttactt gcaatcacta cgttgaaaca agtattgact attcctgaaa
    39361 tcaaagaagg gatattgttc caaggaaaaa cagtaggaat tcgtgaagcc tataatctat
    39421 tttgcgatga acaagaagca gctgtgtgga tggtcagcca attagcacaa ggtaaatctc
    39481 atccgcaaaa attcgaccag gcaacccctg tagaatatat tgctgtaaga gcggcaactt
    39541 tatcgtttgc aatgaaactt cttgcggaaa aaacgattgt tctagaaacc gaatatttaa
    39601 aagaggagaa aacaaatgaa aaacaataaa atcgcaattt tagtagattc aggtacggat
    39661 gttccagaag aattgataaa aaaatacgag atgtatatga tcccattgaa aattatttat
    39721 aaagatcagg tatatacaga taaaattgac attacccctg aagagatcta tcaacgtttg
    39781 ccagaggaaa tccctggtac atctttgccg gatggtgagt cgatcacaga aatctttgag
    39841 caaatcaaaa aagacggtta tgaaaaggta cttgcggtaa caatttccag tggattgagc
    39901 gggacatata atgtggttcg tttaattgca caacagcaag aagaattgga agcatatgta
    39961 ctagatacga aaaatattgg tattggtgca ggtttttctg cgatccaagc agcaaaatgg
    40021 ctggaagaag gagtagaatg ggatcaactg atcagtaatc tgaatgagtt agtcgaaaga
    40081 acaaaagttt tctttaatgt tgcgacattg gaatatcttc aaaaaggtgg acggatcggt
    40141 ttagttgctt ctattgtagg aaccgcttta aagcttaacc caatcatctc atgtaatgaa
    40201 gaagggattt attacacagt aggaaaagct aggggacgta aaaaaagttt ggataaaacg
    40261 gttgcttacg tcaaagaaaa aatcggtaat gcaaaggtct tcaacttagc tgttgcccat
    40321 ggggatgcga aagaagaagc aattgaaatg gaagcgagac ttaaaagaga gttcccacaa
    40381 gcaaaccaaa tctactttgg ccagatttct ccggcattag tagtccacac tggtccggga
    40441 ttattgggtg tcggtgtaca agttttagaa ggataaatca aaaaaaggaa acactgaggt
    40501 gaaaatcagt gttttccttt tttattgaag ttagaacaaa aaataataaa gtattttgca
    40561 ctttcatttt aattttctct tttgttttat aggcaaagat gaaacaaaag cagaaatacg
    40621 gtataatgag aaggtatgac aataatatat aagaggtggc tctttatgaa aaaagtttta
    40681 gttgtcgatg atgagaagcc gatttcggat atcgtcaaat tcaatttagc caaagaaggc
    40741 tatgacgttt atactgcata tgatggggaa gaagccttgg aaaaagttgc tgaggtagaa
    40801 cctgatttga ttttacttga tttgatgcta cctaaaatgg acggcttaga agttgctcgt
    40861 gaggtacgca aaacttatga tatgccgatc attatggtaa cagccaaaga ttctgaaatt
    40921 gataaagtcc taggcttaga gttaggcgcc gatgattacg tgaccaagcc attttccaac
    40981 cgagaattag ttgcacgtgt caaagcaaat cttcgtcgcg gtgccacggc tgcgaaggaa
    41041 ccagaagagg cagcacctgc ggaattgacg attggtgatt tgacgatcca tccagaagct
    41101 tacatggtga cgaaacgagg agaaacaatc gagttaacac atcgtgaatt cgaactactc
    41161 ttttacttgg cgaagcacct tggacaagtg atgacaagag aacatttatt acaaacggtt
    41221 tggggttatg attattttgg agatgtacga acagtggatg taactgtacg tcgtttgcgt
    41281 gaaaagatcg aagataatcc aagccatcca aattatttag tgacacgtcg tggtgtcggt
    41341 tactatttgc gtaacccaga acaggagtaa ttggatgaaa agaaaagtcc gcttttttcg
    41401 atcagtcaat tttaagatcg ctattacctt tattctgatt ttattgatct cgatcgaaat
    41461 tattggtgcc tactttatac gtggtttaga acgttccacg atcaatacat ttattaaaga
    41521 tatgaatcaa acggtggaat cattagcaac aacaatcagc ccggaattga atcgaaaaga
    41581 taatgcagat gatgaagagg taaatgcgaa tatcaaaaga tttattgaaa acagtgcgac
    41641 tagcgatatc atcgaaatac gggtcgttga cgaaaaaggg atcatccgag gaacaaccga
    41701 tgtcaatgaa caaagcgctg tgggaaagaa aaatgattat gttgatatca atgatttcac
    41761 gactaaacgt tatgttgctt tagataacga cgataaacgg gtgaatatca atgttcaacc
    41821 gatcctgtcc ccgaccggcg atgccgttat cggggctctt tacgtcaaaa gtaatatcga
    41881 acaaaaatat tcggaaatca ataacacagc agtcattttc tttactgcct cactaattgc
    41941 ggcatttatt tcaatgatcg tatctgtttt agttgctcgt tcgattacac agccgatcgg
    42001 tgaaatgaga gaacaagcca ttcgaatcgc tagaggagac tacagtcgga aagtcaaagt
    42061 gcatggacaa gatgaacttg gacagctggc tgataccttc aatcagttag ctgaacgtat
    42121 tgaagaagct caagatacaa tggaagctga acgcaatcgt ttggatagcg tgctatccca
    42181 tatgacagac ggcgtcattg cgacagatcg caggggtaaa gtcattacga tcaatgatat
    42241 ggctgtttcc ttgctagata tcaaaaacga ggaagcaatc ggacaatcga ttttgacact
    42301 tttagatatc gaagaagaat atacgttgcg gaaattatta gaagatccaa acgaaatggt
    42361 acttgaccgt tcaaccagta cattggaaag tgatcagatg atcttgcgag tagacttttc
    42421 tatgatccgt cgcgaatcag gttttatcag cggattagta gctgtcttgc atgatgtgac
    42481 agaacaagaa aaaacagaac gggaacgtcg tgaattcgtc tctaatgttt cccatgaact
    42541 tcgtacaccg ttgacaagta tgcgtagtta tatcgaagca ttgagcgaag gtgcgtggaa
    42601 agacgaggaa gtagcaccaa attttttgaa ggtaacgcta gatgaaacag accgaatgat
    42661 tcgaatgatc aatgacctct taaatctatc tcgcatggat acaggtaata cccagcttca
    42721 attagaatat gtcaatttca acgagttagt gaattttgtt cttgaccggt ttgatatgat
    42781 ggtgggaaat caggaaaaaa attataagat ccgtcgtgaa tttacgcaaa gagatctttg
    42841 ggtcgaagta gatacagata aaatcatcca agtagtcgat aatatcatga ataacgcgat
    42901 caagtattca ccagatgggg gaacgattac ttgtcgctta ttagaaacgc ataataacgt
    42961 gattttaagt atcaccgatc aaggattggg gattcctaaa aaagatctga accgagtgtt
    43021 cgagcggttc tatcgagtag ataaagcacg tgcaagagca caaggtggga caggacttgg
    43081 attagctatt tctagagaag tgattaaggc gcatcgaggc gctatttggg cagagagcaa
    43141 ggaaggaaaa gggtcgactt tttatatctc gctgccatat gaaccttatg aggaggaatg
    43201 gtgggaatga gaatctctga aaaagtcgtt cgtataggac tcattttgat ggttgcgtta
    43261 agcatatact tctcttatgc aatctggctg agcccagcag gaaaaagttc aatcaacgta
    43321 gatgagacaa acactcaaat gatcgagtct caaagcaatc gaaaaacttc cgaagtcttt
    43381 cttccgcttc atgttacatg gcaccattcg ggaatgatcc aagaaaccaa cagtgaaaat
    43441 ctcgtgtacc gattgcaatt gatcatcgat aaagcaagat ttggaaagct ttctgaagta
    43501 gtttcaggag acaaatcaca gtttgaacag gtgaaaaatc tgaactctgg ttttgagttg
    43561 gcctataatg cgcctttttt gctaagagaa tacaaggaag catttgattt agatttagat
    43621 gtgtcgaact tggcaaaaga aagcgaaaat atgtatttta ctagaataca attcgatcaa
    43681 ggaaataaaa aaatccgatt tttgaattat tcgaaccatc tcgtatatga agcaaatatt
    43741 tcgttgaatt ggacagatat caataaagaa ttaacagcaa aagacacaaa atggacagaa
    43801 atgacaacag aaccatcgac gatcgacacg caatatgcta cgaaacattc gatcaaactg
    43861 aagaagtata gttatatcct gtcacagcaa ccctatacac tttttagaaa tgcttttttc
    43921 cagaaaccag atagcgtgaa aaataatgat gaaagcaatg aattgatttt ttacggtggg
    43981 aatgaaacaa tgtctatcca ttcggaaacg caagtagttg attttcgtgg agagctgcca
    44041 gagaaaaaag aaaatgctag tgtattttct gaaagtttcc cctatattag caaattaggc
    44101 agttctttag ggaacctcag atattttgac cgcagtgaca atacgatcga ttatcggata
    44161 ttcgtggaag gcttcccggt ttttgggaca gacagcagag gacagattgg tgtggaagtt
    44221 ggcggtgaaa cgaatggttc tgtgcaagtg aatatccaaa ccagtttgaa tacgattcaa
    44281 gtgccgatac cttcagacga agaagtagaa cttgcaagta cgaatgaaat catccagcag
    44341 ttaaaagcta agggagcaga tagcaacaag attggttcag tgatcgttgg ctatacatgg
    44401 caaaatgtca aagaaacgaa tcgtgtcgtt gatctcttac ctgaatggta tgtgaaatat
    44461 aaagataact ggtattcagc taacgacctt ctggaaaaat tacctggatt ggaggcgaat
    44521 taattggatt tcaaaaaaat cgaatggata tttttcgtcg cttttttagg gttgaatgtg
    44581 tttttattca gcagttatca tgacgcggta taccaagaaa acaatgtcat tcgctctaat
    44641 cagactgaat caatccaaca acgtctggct agcgacgata tcacttacac ggggaagatt
    44701 tcgtctgaga acaagcaagg ctattatctt agcgcggagc agaccaatct tagcacttca
    44761 ttagcgaatt atcgcaaggc tggtaaaagc ggcttgctgg caagtggtgt gtttttagac
    44821 ggtgatgttt tgacgaaatc tttatccagt tcagatgctg aaaagaacgt gatcgatcca
    44881 gataaaatta gtagttcctt ggaagatttt ttatctaaca aagacaatat tttattcggt
    44941 aatgattatg tgtacacgaa aaacttttct catttagaag aagaaccact agaaatccaa
    45001 gcaagccaag agtatgaagg catcccattc aatgatgata cagcaaaaat cgttctttct
    45061 ttagaaaaaa acgataatga ttatagcatc acgaaataca tgcagacgca tttatctgat
    45121 atcgagcagc tgcgcgaaaa gacggaactg tatacggaag aggaagcaat caatacattg
    45181 tacatcaata acaaaatacc aagaggatca aaaattttat ggcgtcagct agcttattcc
    45241 tgtattctga aagtccggga aaaaaatgtg tatgttcctg tttggcatgt agcgatcgaa
    45301 acaagcgata aagtggtcca aattgagagc gtcaacgctt tcagcaatac gatcgtgaca
    45361 aacaatactg taccaaaagt ggaagatcaa taaaaaagcg agtataatga aaaaagttta
    45421 caaagtaaga agggaagtag gagtcaaatg gctgaagaaa aaccagcttt taaaatcagt
    45481 atcctagcaa gtggaagttc gggtaattca ctttatatcg aaagtgaaaa aaaacggctt
    45541 ttggtcgatg caggacttag cggtaaaaaa atcacttcct tgatggctca aattgatcgg
    45601 acaccggacc aattagatgg aatacttgtg acgcatgaac atcgagatca tatacatggc
    45661 gtgggtgtct tggctcgtaa gtatcatttg gatgtttttg ccaatcagca gacttgggaa
    45721 gcgatggaac cactgattgg aaatgtacca gtggaacaaa aacatctatt tgagatgggg
    45781 aaagtcagaa cattcggcga tatcgatatt gagagtttcg gcgtctccca tgacgcagcg
    45841 gctccgcaat tttatcgttt ccataaagaa aatcggtcat tcgtcatgtt gacggacacc
    45901 ggatattgca gtgattatat gcgaggtgtc attgaaaatg ccgatggtta tttgatggaa
    45961 agtaatcatg atcttgagat gttacgcatg ggaccttatc cgtggaattt aaaacagcgt
    46021 attttaggag atagaggaca tctttccaac gaagacggtg cacttgtcat gactgatgtg
    46081 atcggtaatc ggaccaaacg aatctatctt ggccatctta gcaaggaaaa caatatgaaa
    46141 gaacttgctc atttaacaat ggaaaatgtt ttgaaagaaa aagattttgg cgttggacat
    46201 gattttgaaa tctatgatac agatccagat gaagcaactg agctattttc gatttaaaca
    46261 gttcaatcaa taaaaatata aaaagcagaa gataaaatca atcagggttg gttttatctt
    46321 ctatttttat ttttttagca tattaaaaaa ataaccgtat tatcgactga ctacactgat
    46381 gtgctgcttt tcatgtttct catccagtat ctcatctact aatgcaatcg cgtaatctgc
    46441 catgctgatc gtactgtcgc tagcagcgtt tctttgtaaa taatcatcac ttaacaaata
    46501 agtacctgta cgtttgccat gtggatcaaa taaagaagct ggactgatat aagtccaatg
    46561 tagattttta gattgtttca attttaacaa tccttcgtac atattgaaag ctgttggata
    46621 gaagggtgca tttttaggta cttggtcaat catacgagtc gtcttttctg tatctaagaa
    46681 taacgaagag gcacccccaa cgacaattaa gcgtgtatcg gttccttcaa gcatctcaat
    46741 caaatgggac aaagaagttt gatgcatttc ttcttttcct ttgggagcgt tgaaagcatc
    46801 aacgacaaca tcaaaagatg caagatcagt agttgtcaac tcaaataaat ccttttgtat
    46861 aaacggaaca tctgatggca atttttctgg atgacgcacg attgccgtca cgtctaaatc
    46921 tctggacaat gcttcgtcta aaataaatga acctgcatgg ccagtagctc caatgagtgc
    46981 aattttcata aaaaacctcc ttttttattc ctgtccattg taagcagttg ctgaatattc
    47041 gtcaaagaat ccgatttttg aaaagctcag tgcgcattcc tttcatggtt cgtgatcatt
    47101 gggtatagtt aaattaagaa aactgattga taaaaataga agttgaagga gataagaaat
    47161 gaaagagtgg ttagatcgtg cctttttacc aatgataaca tctgttgtga atcttagacc
    47221 cttaactgct ttgcgtaatg gaatgactta cacgatccca ataattattg ttggctcgct
    47281 gtttttctta tttgctcatt ttcctgtgga ttacatcgct cagtggatag acaaaacagg
    47341 attttctatg tattttacac aagtatacca ggctacattt gatgtgatag ccttttggat
    47401 cgtttttgcc attgcatacg cttatgcaaa agaagaagac atcgatcgct taccagtcgg
    47461 cataacagcc ttgtctgctt atctactgct tattcagcca ctaaatactc agatagatac
    47521 tgtctggaca ggtgctgaag ggatactagg agcagttaca actgggctca tcatcggatg
    47581 gggctatgca cgtttagtaa gacttgcgaa agggtggaag caaagagaga agatacctga
    47641 aaatgtttgg cagtctttta cttcattgat tccaatcatt gttatagtca cttttacgct
    47701 tatcggttca gcggtttttc aacatttgtt cagaatgact gttgttcaag gagtatggac
    47761 atatgtccag cttcccattc aatcgttcat agacacgttg tttggtgtgc ttttattagg
    47821 tttttttgtt ccgtttttat ggttatttgg tcttcatgga tctgcgatgg tcaatgggtt
    47881 ggttagtccc gtcttgcaag cgaattcatt agcaaatgca gaaatcttag cctcaggaca
    47941 agagttgacg gtggcaaatg gcggacatat cgttactcag caatttctcg atcagttcat
    48001 gacggttacg ggtgctggat tgacgttagg ggctgtattt tttatgacgg tttttgcgaa
    48061 atcgaagaaa tacagagaat taggaaatct tgctctttta ccagcctttt tcaacatcaa
    48121 tgaatcaatt attttttcca caccgatcgt gatgaacccg atgatggctg ttccttttat
    48181 tttcgcaccg gtcgtttcag gattgattac ctattccgct ttatattttg gattcgttcc
    48241 tttatttaca gcagtccaag tcccttggac aacaccgccg atcctctcgg gtttcttagt
    48301 aggcggagtg cctgcagcga ttttacaagg tatcgtgttg tcaattagtt ttttcattta
    48361 tttcccattt ttgaaaaaaa tagattctgc aaactgcaaa aaggaaagac agacaaaaaa
    48421 tgatggagca gcagaaaaga tgaacgacta aaaaacaaaa atactcctgt cgctttagat
    48481 tgggaaaatc accaatctag gatgacagga gtatttatag tcatgtttat actagccctt
    48541 tttggacagc taattgaaca agaacaacta atgtgttcat tcctacgtgt gtgatcatcg
    48601 atgtccagat ccgaccagtt gctttgtact ctaggctgaa gaagaaacct aggaaaaagt
    48661 agattagcag atgaccatca ttatgagcaa acgcaaacag caaagagctg atcactgctc
    48721 cgacccaaaa atttgaatat cgactgatga tccccagtat actgcggcgg aatacgaact
    48781 cttccatgat gggtccagca acagttgtcg ccaaaataaa agcgggtgct tccatgatca
    48841 tctggatgat attttgtgta ttctcggaag gggttgcttt gccaaacaat acagattcta
    48901 acatgacagc gatgctttgc agaatcaatg cgacaaagat tccaaccaat ccataaataa
    48961 caatctttgg agcagaagct ttttttaccg ctgattcgat cgctaagggt tcacgttgct
    49021 taaaataaaa aatagctagg acaacggcgc ctagtatata catgactgca gttgctgtga
    49081 tcaattcaga cgtagatttt acaagtccta tcgaagctag gatcaagggt gagataaaaa
    49141 ttagaccata gcaaaaaatg ctgaggaagc tatatttttt taaagacatg taattcctcc
    49201 ttttaaggga taacaagcat gcaaggcaag cttatatcga ttgatttcaa ttatatcata
    49261 atttcaataa gcagctgtca gaataaaatt tcttttcttt gatttaaaag cttgcaaaaa
    49321 agagggagaa tgagtattat taaaaatgta ggttagcact cgaaggttaa gagtgctaac
    49381 agattaaaaa aagagttatc ggagggattt atcgtgttaa aaccattagg tgatcgcgtc
    49441 attatcgaag tcgcaaaaga agaagaaaaa actgtcggag gaatcgtttt agcttctgct
    49501 gctaaagaga aaccccaaac tgggacagtt gttgcagtcg gtgaaggccg tttgttagaa
    49561 aacggtgaaa aagttcctgc cgctgtaaaa gcaggcgacc aagttatgtt cgaaaaatac
    49621 gccggtacag aagtaaaata cgaaggaaaa gaatatctga tcgtcgcagg aaaagatatc
    49681 atggcgattg ttgagtaaat ttggaagata aaaaaataat caaaaataat caatgaggtg
    49741 aatcaatcat ggcaaaagaa ttgaaatttg cagaagacgc acgcgcagct atgttacgcg
    49801 gggtagataa attagcagat acagtgaaag taacattagg acctaaaggc cgaaatgtcg
    49861 tactagaaaa atcttacggc tctccattga tcacaaatga tggtgtgacg atcgcaaaag
    49921 aaatcgaatt agaagatcat tttgaaaaca tgggtgcaaa acttgtatca gaagtagcat
    49981 caaaaacaaa tgatattgca ggagacggta caacgacagc aactgttctg acacaagcaa
    50041 tcgttcgcga aggtttaaaa aacgtaactg caggtgctaa tcctctaggc attcgtcgcg
    50101 ggatcgaatt ggcaacaaaa gcagcagtcg aagaattgca taatatctca actgttgttg
    50161 attcaaaaga agccattgca caagtagcag ctgtttcttc gggttctgat aaagttggac
    50221 atttgattgc tgatgcaatg gaaaaagttg gaaacgacgg tgtcatcaca atcgaagaat
    50281 caaaagggat cgaaacagaa ctagatgttg tagaaggtat gcagtttgat cgtggttact
    50341 tatcacaata tatggtaaca gataacgaca aaatggaagc tgttctagaa aacccatata
    50401 tcttgatcac agacaaaaaa atctctaaca tccaagatat tctgccattg ttagaacaaa
    50461 tcttgcaaca atcacgtcca ttgttgatta ttgctgacga tgtagatggt gaagcattgc
    50521 caactcttgt attgaacaaa atccgtggaa cattcaacgt agtagctgta aaagctcctg
    50581 gtttcggcga tcgtcgtaaa gcaatgcttg aagatatcgc tattttgaca ggtggtacag
    50641 taattactga cgatctaggt ttagaattga aagatgcaac aatcgaaaac ttaggaaatg
    50701 cttctaaagt tgttgtagat aaagacaata caacgatcgt agaaggttct ggagaaaaag
    50761 aagcaatcga agctcgtgtg caattgatca aaaaccaaat tgctgaaaca acttctgatt
    50821 ttgaccgcga aaaattacaa gaacgcttag ctaaattagc aggtggtgta gctgttgtta
    50881 aagttggtgc agctactgaa acagaattga aagaattaaa attacgcatt gaagatgcat
    50941 tgaatgctac acgtgccgca gtagaagaag gaatggtctc aggtggtggt acagctctag
    51001 tgaacgttat cagcaaagtt tctgctgtag aagcagaagg agacgtggca acaggtatca
    51061 agatcgttgt tcgtgcttta gaagaaccaa ttcgtcaaat cgctgaaaac gctggttatg
    51121 aaggatcagt gattgttgat aaattgaaga acgtggaact aggcactggt ttcaatgctg
    51181 ctactggaga atgggtaaac atggttgaag caggtatcgt agacccaact aaagtaacac
    51241 gttctgcctt gcaaaatgcc gcttccgttt ctgccttgtt attaacgaca gaagcagtcg
    51301 ttgcagataa accagaacca gcagcgccag cagctcctgc aatggatcca tcaatgatgg
    51361 gcggtatgat gtagtaatta agaaagaacc ctattttaac agggttcttt cttctttttt
    51421 tgttttattt taagcaaaag gggcaaagaa ggggcaaaaa
//
LOCUS       DACDXO010000014        51083 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E23605_2017
            SAMN12639989-rid9881463.denovo.014, whole genome shotgun sequence.
ACCESSION   DACDXO010000014 DACDXO010000000
VERSION     DACDXO010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639989
            Sequence Read Archive: SRR10023389
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 51083)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51083)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741914.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:42:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,972
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 33 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 17 of 73
            Pseudo Genes (multiple problems)  :: 10 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51083
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639989-rid9881463.denovo.014"
                     /isolate="E23605_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..683
                     /locus_tag="IWM35_000953"
     CDS             <1..683
                     /locus_tag="IWM35_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019117172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HAP9918819.1"
                     /translation="KEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVSPLPLGSG
                     MQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYSPVSTPAD
                     FRMLTPIVLEQAFRKAGTELLEPYLSFKVYAPQEYLSRAYNDAPKYCANIVNTQLKNN
                     EVIIIGEIPARCIQDYRNDLTFFTNGLSVCLAELKGYQVTTGEPVCQTRRLNSRIDKV
                     RYMFNKIT"
     gene            781..969
                     /locus_tag="IWM35_000954"
     CDS             781..969
                     /locus_tag="IWM35_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP9918820.1"
                     /translation="MINYKWILCPICGNKTRLRIRVDTILKNFPLYCPKCKNETLINV
                     QKMNIITIKEPDAKTQSR"
     gene            complement(1027..1380)
                     /locus_tag="IWM35_000955"
     CDS             complement(1027..1380)
                     /locus_tag="IWM35_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9918821.1"
                     /translation="MRKKEDKYDFRAVGLAIKEARMKRGLTREQVGTMIEIDPRYLTN
                     IENKGQHPSTQVLYDLVSLLHVSIDEFFLPTDNLIKSTRRLQVEKYMDSFTDKELSLM
                     EALAKGINEARNIEG"
     gene            1911..2333
                     /locus_tag="IWM35_000956"
     CDS             1911..2333
                     /locus_tag="IWM35_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAP9918822.1"
                     /translation="MKPSSFQETIENQFDYICKQVIEDERKDYFKHLDRLSAKEVSFS
                     DIDNYLVNSFSTVDTYVTDFQLFKLFGLRINVESDLLSEALRHLSEKKRNIILLHYFM
                     DMSDVEIAELLNLNRSTVYRHRISGLAMIKEFMKECKE"
     gene            2330..2560
                     /locus_tag="IWM35_000957"
     CDS             2330..2560
                     /locus_tag="IWM35_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9918823.1"
                     /translation="MKTAYPRVPFPLIVKATDGDVEAINQIVKHYRGYTSKRSLRRMT
                     DEYGNSHMVIDETLRGRMETKLISKILSFEIK"
     gene            3058..3258
                     /locus_tag="IWM35_000958"
     CDS             3058..3258
                     /locus_tag="IWM35_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008475635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAP9918824.1"
                     /translation="MITVTKKDLVELGYGTSFSADIIKKAKELMITKGHIYYQSRKLD
                     RVPREAVEEILGIRFSNENRSE"
     gene            3285..4478
                     /locus_tag="IWM35_000959"
     CDS             3285..4478
                     /locus_tag="IWM35_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP9918825.1"
                     /translation="MVKDPIKKAKNGTYYFRANLGYDSKGKKIQKYRSGFTTKKEARE
                     EYSKLLLMKPEELSENKDKMTFKHYIFEIFLPWYKTQVKLRTYENRLPTIKKHFSYFD
                     KMAVSDIEPIDVQNWQLKLSKKCKSSYVRAVQGLFSVAMDRAIVLGITTTNPSKIIGN
                     VKKQKSKIEFWTKEEFEKVISLIYKEDYYQHFLFISLWFLFMTGMRIGEATAIQWEDI
                     DFDSGVLTINKTLYYKNQSNYRFVEPKTKASVRHIVLDKCTLTYLSEWKEVQQNLIQT
                     DFVMSYNGIPTQKHTLSYAIERYAKLAGIHRIRIHGLRHSHASLLISMGENPLIIKDR
                     LGHEDIETTLGTYGHLYPNSNFEVANKLNGAISFKEAKQNYDTSPKNQFTVDYLRNKE
                     MKKVQ"
     gene            complement(4546..6111)
                     /gene="guaA"
                     /locus_tag="IWM35_000960"
     CDS             complement(4546..6111)
                     /gene="guaA"
                     /locus_tag="IWM35_000960"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HAP9918826.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVDARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(6170..7093)
                     /locus_tag="IWM35_000961"
     CDS             complement(6170..7093)
                     /locus_tag="IWM35_000961"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HAP9918827.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            7289..7894
                     /locus_tag="IWM35_000962"
     CDS             7289..7894
                     /locus_tag="IWM35_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP9918828.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            8202..9191
                     /locus_tag="IWM35_000963"
     CDS             8202..9191
                     /locus_tag="IWM35_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP9918829.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIR"
     gene            9359..10660
                     /locus_tag="IWM35_000964"
     CDS             9359..10660
                     /locus_tag="IWM35_000964"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HAP9918830.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            10706..11368
                     /gene="deoC"
                     /locus_tag="IWM35_000965"
     CDS             10706..11368
                     /gene="deoC"
                     /locus_tag="IWM35_000965"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HAP9918831.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            11389..11787
                     /locus_tag="IWM35_000966"
     CDS             11389..11787
                     /locus_tag="IWM35_000966"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HAP9918832.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            11853..12935
                     /locus_tag="IWM35_000967"
     CDS             11853..12935
                     /locus_tag="IWM35_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP9918833.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            13147..14709
                     /locus_tag="IWM35_000968"
     CDS             13147..14709
                     /locus_tag="IWM35_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9918834.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            14702..15835
                     /locus_tag="IWM35_000969"
     CDS             14702..15835
                     /locus_tag="IWM35_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9918835.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            15835..16791
                     /locus_tag="IWM35_000970"
     CDS             15835..16791
                     /locus_tag="IWM35_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9918836.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            16890..18056
                     /gene="deoB"
                     /locus_tag="IWM35_000971"
     CDS             16890..18056
                     /gene="deoB"
                     /locus_tag="IWM35_000971"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HAP9918837.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            18080..18898
                     /locus_tag="IWM35_000972"
     CDS             18080..18898
                     /locus_tag="IWM35_000972"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HAP9918838.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            18915..19622
                     /gene="deoD"
                     /locus_tag="IWM35_000973"
     CDS             18915..19622
                     /gene="deoD"
                     /locus_tag="IWM35_000973"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HAP9918839.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
     gene            complement(19920..20606)
                     /gene="gpmA"
                     /locus_tag="IWM35_000974"
     CDS             complement(19920..20606)
                     /gene="gpmA"
                     /locus_tag="IWM35_000974"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HAP9918840.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(20778..21359)
                     /locus_tag="IWM35_000975"
     CDS             complement(20778..21359)
                     /locus_tag="IWM35_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP9918841.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(21492..22175)
                     /gene="rpiA"
                     /locus_tag="IWM35_000976"
     CDS             complement(21492..22175)
                     /gene="rpiA"
                     /locus_tag="IWM35_000976"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HAP9918842.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            22494..22907
                     /gene="rpsL"
                     /locus_tag="IWM35_000977"
     CDS             22494..22907
                     /gene="rpsL"
                     /locus_tag="IWM35_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007085295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HAP9918843.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            22991..23461
                     /gene="rpsG"
                     /locus_tag="IWM35_000978"
     CDS             22991..23461
                     /gene="rpsG"
                     /locus_tag="IWM35_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HAP9918844.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            23555..25642
                     /gene="fusA"
                     /locus_tag="IWM35_000979"
     CDS             23555..25642
                     /gene="fusA"
                     /locus_tag="IWM35_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HAP9918845.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            25814..27001
                     /gene="tuf"
                     /locus_tag="IWM35_000980"
     CDS             25814..27001
                     /gene="tuf"
                     /locus_tag="IWM35_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAP9918846.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
     gene            27408..27716
                     /gene="rpsJ"
                     /locus_tag="IWM35_000981"
     CDS             27408..27716
                     /gene="rpsJ"
                     /locus_tag="IWM35_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HAP9918847.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            27747..28376
                     /gene="rplC"
                     /locus_tag="IWM35_000982"
     CDS             27747..28376
                     /gene="rplC"
                     /locus_tag="IWM35_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HAP9918848.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRHHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            28404..29027
                     /gene="rplD"
                     /locus_tag="IWM35_000983"
     CDS             28404..29027
                     /gene="rplD"
                     /locus_tag="IWM35_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HAP9918849.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            29027..29317
                     /gene="rplW"
                     /locus_tag="IWM35_000984"
     CDS             29027..29317
                     /gene="rplW"
                     /locus_tag="IWM35_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HAP9918850.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            29356..30189
                     /gene="rplB"
                     /locus_tag="IWM35_000985"
     CDS             29356..30189
                     /gene="rplB"
                     /locus_tag="IWM35_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010747214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HAP9918851.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            30232..30510
                     /gene="rpsS"
                     /locus_tag="IWM35_000986"
     CDS             30232..30510
                     /gene="rpsS"
                     /locus_tag="IWM35_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HAP9918852.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            30533..30880
                     /gene="rplV"
                     /locus_tag="IWM35_000987"
     CDS             30533..30880
                     /gene="rplV"
                     /locus_tag="IWM35_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HAP9918853.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            30894..31550
                     /gene="rpsC"
                     /locus_tag="IWM35_000988"
     CDS             30894..31550
                     /gene="rpsC"
                     /locus_tag="IWM35_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HAP9918854.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            31553..31987
                     /gene="rplP"
                     /locus_tag="IWM35_000989"
     CDS             31553..31987
                     /gene="rplP"
                     /locus_tag="IWM35_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HAP9918855.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            31977..32165
                     /gene="rpmC"
                     /locus_tag="IWM35_000990"
     CDS             31977..32165
                     /gene="rpmC"
                     /locus_tag="IWM35_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HAP9918856.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            32190..32456
                     /gene="rpsQ"
                     /locus_tag="IWM35_000991"
     CDS             32190..32456
                     /gene="rpsQ"
                     /locus_tag="IWM35_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HAP9918857.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            32514..32882
                     /gene="rplN"
                     /locus_tag="IWM35_000992"
     CDS             32514..32882
                     /gene="rplN"
                     /locus_tag="IWM35_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HAP9918858.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            32919..33227
                     /gene="rplX"
                     /locus_tag="IWM35_000993"
     CDS             32919..33227
                     /gene="rplX"
                     /locus_tag="IWM35_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HAP9918859.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            33255..33794
                     /gene="rplE"
                     /locus_tag="IWM35_000994"
     CDS             33255..33794
                     /gene="rplE"
                     /locus_tag="IWM35_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HAP9918860.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            33813..33998
                     /locus_tag="IWM35_000995"
     CDS             33813..33998
                     /locus_tag="IWM35_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HAP9918861.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            34034..34432
                     /gene="rpsH"
                     /locus_tag="IWM35_000996"
     CDS             34034..34432
                     /gene="rpsH"
                     /locus_tag="IWM35_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HAP9918862.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            34464..35000
                     /gene="rplF"
                     /locus_tag="IWM35_000997"
     CDS             34464..35000
                     /gene="rplF"
                     /locus_tag="IWM35_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HAP9918863.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            35161..35517
                     /gene="rplR"
                     /locus_tag="IWM35_000998"
     CDS             35161..35517
                     /gene="rplR"
                     /locus_tag="IWM35_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014125626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HAP9918864.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            35538..36038
                     /gene="rpsE"
                     /locus_tag="IWM35_000999"
     CDS             35538..36038
                     /gene="rpsE"
                     /locus_tag="IWM35_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HAP9918865.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            36053..36232
                     /gene="rpmD"
                     /locus_tag="IWM35_001000"
     CDS             36053..36232
                     /gene="rpmD"
                     /locus_tag="IWM35_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HAP9918866.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            36276..36716
                     /gene="rplO"
                     /locus_tag="IWM35_001001"
     CDS             36276..36716
                     /gene="rplO"
                     /locus_tag="IWM35_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HAP9918867.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            36716..38011
                     /gene="secY"
                     /locus_tag="IWM35_001002"
     CDS             36716..38011
                     /gene="secY"
                     /locus_tag="IWM35_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HAP9918868.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            38073..38720
                     /locus_tag="IWM35_001003"
     CDS             38073..38720
                     /locus_tag="IWM35_001003"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HAP9918869.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            38911..39129
                     /gene="infA"
                     /locus_tag="IWM35_001004"
     CDS             38911..39129
                     /gene="infA"
                     /locus_tag="IWM35_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HAP9918870.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            39162..39278
                     /gene="rpmJ"
                     /locus_tag="IWM35_001005"
     CDS             39162..39278
                     /gene="rpmJ"
                     /locus_tag="IWM35_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HAP9918871.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            39296..39661
                     /gene="rpsM"
                     /locus_tag="IWM35_001006"
     CDS             39296..39661
                     /gene="rpsM"
                     /locus_tag="IWM35_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HAP9918872.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            39689..40078
                     /gene="rpsK"
                     /locus_tag="IWM35_001007"
     CDS             39689..40078
                     /gene="rpsK"
                     /locus_tag="IWM35_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HAP9918873.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            40158..41096
                     /locus_tag="IWM35_001008"
     CDS             40158..41096
                     /locus_tag="IWM35_001008"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HAP9918874.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            41124..41504
                     /gene="rplQ"
                     /locus_tag="IWM35_001009"
     CDS             41124..41504
                     /gene="rplQ"
                     /locus_tag="IWM35_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HAP9918875.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            41776..42615
                     /locus_tag="IWM35_001010"
     CDS             41776..42615
                     /locus_tag="IWM35_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAP9918876.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMIFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            42591..43460
                     /locus_tag="IWM35_001011"
     CDS             42591..43460
                     /locus_tag="IWM35_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAP9918877.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            43450..44247
                     /locus_tag="IWM35_001012"
     CDS             43450..44247
                     /locus_tag="IWM35_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HAP9918878.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
     gene            44450..45196
                     /gene="truA"
                     /locus_tag="IWM35_001013"
     CDS             44450..45196
                     /gene="truA"
                     /locus_tag="IWM35_001013"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HAP9918879.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKIRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
     gene            complement(45257..45463)
                     /locus_tag="IWM35_001014"
     CDS             complement(45257..45463)
                     /locus_tag="IWM35_001014"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9918880.1"
                     /translation="MVTEKIGNKKMIHLSTPRPQVTIFIKDEKLFFEIALCDSELNDR
                     LTTSFLNGVPEVASSKISRNFAKF"
     gene            complement(45529..48198)
                     /locus_tag="IWM35_001015"
     CDS             complement(45529..48198)
                     /locus_tag="IWM35_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HAP9918881.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFVYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDGGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEISFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEQPRNTHRRRRRK"
     gene            complement(48436..48624)
                     /locus_tag="IWM35_001016"
     CDS             complement(48436..48624)
                     /locus_tag="IWM35_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HAP9918882.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
     gene            48894..49256
                     /locus_tag="IWM35_001017"
     CDS             48894..49256
                     /locus_tag="IWM35_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAP9918883.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            49308..50990
                     /locus_tag="IWM35_001018"
     CDS             49308..50990
                     /locus_tag="IWM35_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HAP9918884.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKEIPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
BASE COUNT        16497 a         9347 c        10928 g        14311 t
ORIGIN      
        1 taaaagagcc tacagtcatt tatatggaga gaccgttaaa aaatgcagaa tataccattc
       61 acatcgaagt gccgccaaat cctttctggg cttccattgg tttatctgta tcaccgcttc
      121 cgttgggaag tggaatgcag tatgagagct cggtttctct tggatactta aatcaatcat
      181 ttcaaaatgc agttatggaa gggatacgct atggttgcga acaaggatta tatggttgga
      241 atgtgacgga ttgtaaaatc tgttttaagt acggtttata ctatagccct gttagtactc
      301 cagcagattt tcggatgctt actcctattg tactggagca agcctttaga aaagctggaa
      361 cagaattgtt agagccatat cttagtttta aagtttatgc accacaggaa tatctttcac
      421 gggcatataa cgatgctccc aaatattgtg caaatatcgt aaatactcaa ctgaaaaata
      481 atgaggtcat tattattgga gaaattcctg ctcgatgtat tcaagattat cgcaatgatt
      541 taactttttt tacaaatggg cttagtgttt gtttagcaga gctaaaagga tatcaggtta
      601 ccactggcga acctgtttgc cagacccgtc gtctaaatag tcggatagat aaagtaagat
      661 atatgttcaa taaaataact tagtgcgttt tatgttgtta tataaatatg gtttcttatt
      721 aaataagatg aaatattctt taatatagat ttgaattaaa gtggaaagga ggagattgtt
      781 attataaact acaagtggat attgtgtcct atttgtggaa ataaaacaag actacgaata
      841 cgagtggata ctatacttaa aaatttccct ttatactgcc ccaaatgtaa gaacgaaact
      901 ttaattaatg ttcaaaaaat gaatataata acaatcaaag agccagacgc caagacgcag
      961 agccgataat ttgagaaatg aaactctcat cttatcggct ctttttgttt atctgaattt
     1021 tactgactag ccttcaatat ttcttgcttc attgatacct tttgctagtg cttccattaa
     1081 ggatagttct ttgtctgtaa agctatccat gtatttttct acctgtaacc ttcgagtgct
     1141 tttaatcaaa ttatctgtag gtaagaaaaa ttcatcaata gatacatgga gtaatgatac
     1201 aagatcatac agcacttgtg tacttgggtg ttgtcctttg ttttctatat ttgttaaata
     1261 acgtgggtca atttcaatca ttgttcccac ttgttcacga gtgagacccc gtttcattcg
     1321 agcctcttta atcgctaaac caacggctct aaaatcgtat ttatcttctt ttttacgcat
     1381 actaaatcac ctctatacat tttactgttc ctatataacc ataaacaggt atagaaaaac
     1441 atgttatata gtttatgagt tcctatagaa cactgtggaa tttattaatg cgatgaatcc
     1501 aatttcaaat agaaaggtcg ctgattttat ttacctatac aactttataa aatttagtca
     1561 tactcctaga aagtagataa aattggcgac ttaatggagg gaaaagtcgc catgagaaag
     1621 ataatctgtc atctagttct cttaattaag ttcttatcaa aaaaacttat ccacctgtac
     1681 tggatagata tgaccatcaa tgtggttttg cagattacat agatagaaaa atcaaagcat
     1741 atgaacagag aaaacaatct gtttatgtgc ttttttggtc acttaataaa tatcttttag
     1801 gatttctctt catttatgca acaaatcccc cgttcgtgtc ggggtttagt atgaagggag
     1861 ataaagaagc acatgccact ccctttcacg acagaaaggg ggtgaaaagc atgaaaccat
     1921 cttcttttca agaaaccata gaaaatcagt ttgactatat ctgcaaacaa gttattgaag
     1981 atgaacgaaa agattacttc aaacatctag acagactatc agcaaaagaa gtctcgtttt
     2041 ctgatataga taactacctt gtcaactctt tttctactgt tgatacgtac gtcactgatt
     2101 tccaactatt taaattattt ggattaagaa tcaatgttga aagtgattta ttaagtgaag
     2161 ccctgcgtca tctttcagag aaaaaacgga acattatttt acttcattac tttatggata
     2221 tgagtgatgt ggagattgcg gagttactaa atttgaatcg ttcgactgtt tatcgtcatc
     2281 gaataagtgg actagccatg attaaagaat tcatgaagga gtgtaaagaa tgaaaacagc
     2341 ttatccaagg gttcccttcc ccctcattgt aaaggcgaca gatggcgacg tggaagcaat
     2401 taatcagatt gttaaacatt atcgagggta tacctcaaaa cgttcattac gtcgcatgac
     2461 agacgaatat ggaaattctc atatggttat tgatgaaacc ctacgtggca gaatggaaac
     2521 gaaactcatc tctaagattt tatcgttcga aattaaatag tcccattttt tcgtggaagc
     2581 gtgtcgaatc gtatccacgc ttcccgattt gggaggtttg ttattccatc aaagcatatt
     2641 gtgtgttcag taggttttga taggctaaca agccaaacgt tctttgacaa ccaaataaag
     2701 gcaatcgaat acgtttcggt aagaaaagag ctgatgaact aacacgccat gacctacaaa
     2761 attagtgagc gattcatgtt agtaattcga aagtaatctt tagcaggatt gctgccatga
     2821 ctttcttatc gtgataatga tactcccgta tagccatagt ccgagcgtta gaagcgtcac
     2881 aggcaatggg tacggctaca tgagaaccat gcagaagtga aattcctgtg agctttgcta
     2941 gagctgttcg attgctaaga tattcttctc ttgtcagtgt attattataa aactatttga
     3001 tataattata ggtgaatatt ggagttttcc tagcctataa ttgggaaagg agtaataatg
     3061 ataacggtca caaaaaagga tttagttgag ctaggatatg gaacttcttt ttcagcagac
     3121 atcataaaaa aagcaaaaga attgatgatt acaaaagggc atatctacta tcaatctcga
     3181 aagctagata gagtccctcg tgaagctgtt gaagaaattc taggaattcg gttttcaaat
     3241 gaaaacaggt ctgagtagtt tttgcacagt aaggagtaaa aactatggtt aaagacccaa
     3301 taaaaaaagc aaagaatgga acctattatt ttcgtgcaaa tctaggttat gattctaaag
     3361 ggaaaaaaat tcaaaaatat cgtagtggat tcactactaa aaaagaagca agagaagaat
     3421 attcgaaact gctattgatg aaaccagaag aattaagtga gaataaggat aaaatgacgt
     3481 ttaaacatta tatctttgaa attttccttc catggtataa gacacaggta aagctcagaa
     3541 catatgaaaa tcgcttacca actattaaga agcatttctc ttattttgat aaaatggctg
     3601 tttccgatat tgagcctatt gatgtgcaaa attggcaact aaaattatca aagaagtgta
     3661 agtcttctta tgtaagagct gttcaaggac tattttcagt tgctatggat cgagcaattg
     3721 tccttgggat aaccaccaca aatccatcta agattattgg caatgtgaaa aagcaaaagt
     3781 ctaaaattga attttggaca aaagaagaat ttgaaaaagt aatctctcta atttacaaag
     3841 aagattacta tcaacacttt ttatttatct ctctgtggtt tttatttatg actggaatgc
     3901 gaattggtga agctactgcc attcaatggg aagatattga ttttgactca ggagtactaa
     3961 ccatcaacaa aacactctat tacaagaatc aatcgaatta tagatttgtt gaaccaaaaa
     4021 caaaggcgag cgttcgtcat attgtattag ataaatgcac attaacttat ttatccgaat
     4081 ggaaggaagt acaacaaaat ctaattcaga cagactttgt aatgagctat aacggtattc
     4141 ctacccaaaa acatacacta tcatatgcta ttgaacgcta tgctaagctg gctggtatcc
     4201 atcgaatcag aatccatggg ttacggcatt ctcatgcttc cttgttaatc agtatgggag
     4261 aaaatccact tatcattaaa gatcgtctag gacacgaaga tattgagacg actttaggaa
     4321 cgtatggaca tctttatcct aacagtaatt tcgaggtagc taacaaatta aatggagcca
     4381 tttcctttaa agaagcaaaa caaaattatg atacttcacc aaagaatcaa tttactgtag
     4441 actacttgcg aaacaaagaa atgaaaaaag tgcaataaaa gtgcagtaaa aagataagaa
     4501 gagccagaaa cccttggtat aagaggattt ctagctctcc tacaactatt cccactccac
     4561 tgttgctggt ggtttagaag tgatgtcata cacgatacgg ttgacgtggg ctacttcgtt
     4621 tacgatacgt acagagattt tttgcaagac ttcccatggg atacgggcaa agtcagcggt
     4681 cattccgtcg attgaagtta cagcacggat accgactgtg tagtcgtaag tacgtccgtc
     4741 acccataaca ccgacagagc ggatccctgg aagaacagtg aaatattgcc agatatcacg
     4801 gtcaagtcca gcagctgcga tttcttcacg caagatagca tcagattcgc gtacgatttc
     4861 tagcttgtct tcagtgattt cgccaagaac acgaatacca agaccaggac cagggaatgg
     4921 ctgacgccaa acgattgaat caggcatgcc taactgagtc cctaattcac ggacttcgtc
     4981 tttgaataat gtgtttaatg gttcgatcaa ttcaaattgc atatcttctg gcagtccacc
     5041 tacattatgg tgagatttga tcgtctgtgc tgtttctgta ccagattcga taacgtcagt
     5101 atacaacgtt ccttgtgcta ggaaagagac gccttcttca ccagcaagtt ttgttgcttc
     5161 gtcatcaaaa acatatacga attcattccc aatgatttta cgtttttgtt ctgggtcaga
     5221 tacacctttc aacttgtcta agaagcgatc tcttgcatcg actttgatga tgttcaagcc
     5281 gaattttccg cctagacttt ccattacttg ttccgcttcg cctttgcgta gcagaccgtg
     5341 gtcaacaaag atagatgtca attggtcacc gatcgctttt tgtaaaagaa ccccaacgac
     5401 actagaatca acgccacctg aaagaccaag caagactttt ttgtcaccaa cttgttcacg
     5461 gattttttct acttgcatat cgataaagtt atccattgtc cagttacctt cacagtgaca
     5521 aacatcaaag gcaaagtggc gcagcaattc gattccgtat tcggaatgac gtacttctgc
     5581 atggaattgg ataccataaa aattacgtgt tgtgtcttgg atagaagcaa tcggacaatc
     5641 tttgcttgtt ccgactgttt caaagccttc aggaacttta gttaccaagt caccatggct
     5701 catccatact gtttgttgtt ttggtgtttc tttaaacaag acagcttgat cgcttgttac
     5761 ttctaacaag gctttcccat attcgcggtt tttagcaggc tctacggaac cgccaagttt
     5821 gtacgtgatc aattgcatac catagcaaat ccctaataca ggaataccta aattgaagat
     5881 ctcaggatcg atcccaaatg cattctcatc atatacactg tttggtccgc cagaaagaat
     5941 gattcccttt ggattcatcg cttttacttc ttctgcagta atgcggtggc tcaataattc
     6001 ggaaaagact ccaagatcac gaatacgtct tgtgatcaat tggttatact gactaccgta
     6061 gtctaatacg ataatctttt caacgttcgt catatcggca acgtttgtca aatttattac
     6121 ccctatccct ttaaatttta tctcaaaata gtcaaactat tttaaaatgc taatattttc
     6181 gcatgaaaat cgaatcgatg atatgatttt cagttttatg gagaattata tcggctcttc
     6241 ctcgtgtagg caagatatat tcctgtaaat ttttcaaatt cacatttttc catacatctt
     6301 ttgccatttt aaaggcttct tcacgatctc cgatagcaaa acgataataa taattctctg
     6361 gattttggaa agccgtatcc agtaacgcac caaaacgatc caaataccat ttttcgatca
     6421 gcacgggatc ggcatcgaca tagacagaaa aatcaaaaaa gtcgctgacg tagatttgct
     6481 gattcgcagg taattgaagc gtattgatcc cctcaacgat caagatatca ggctgctgga
     6541 tcaattcgta cttgtctgga atgatatcat acacatcatg tgagtaaaca ggtactttta
     6601 tctcttcctg accagatttt acacggttca agaaatcgat cagcatctcc atatcataac
     6661 tttcaggaaa tcctttacgg tccatgattc cacgctcttt taatatacga tttggataaa
     6721 gaaatccgtc agtcgtgatc agctgaacgt ttcttcgttt gaaggtacga gacaaaatcg
     6781 tctgcagcag tctggctgtt gtactttttc caacggctac acttccggcg atcccgataa
     6841 taaaaggcgg tacttctacg tatttatgaa gaaataaacc tttactcaag aacaaagatt
     6901 caaattcttt catataaaga taaatcagat gagcaagagg tacatagata tctcgaacat
     6961 cctgcatgga aatgcggtca ttcaaacttt tgatattatc tagttcatct tgtgtcaaag
     7021 gtgcttgccc attacgataa aattctcgcc attcttctct tgccacacgg tagtagttca
     7081 ttggatcatt catattagcc tcctgcgttc tcccatcaat ggaactattt taacacaaaa
     7141 taaacgcaaa ccccaccact taagagagat tgccgatctt tttgtataat ctttataata
     7201 gaaagaaacg gagtcttatt cttgacttgc gcacggatac ataaaaggat agactgttgt
     7261 catgaaagaa atgagaagga tggaagacat gacaaatcat tactattctg aacgcccaga
     7321 tacggcgcac gattttgaac aatgggcttt tgagttaaaa ggcagaacct ttcaatttgt
     7381 aacagattct ggtgtatttt cacgtgaaac agtggattat ggttcacgtg tcctgattga
     7441 tgctttcgat tgggaagagt tacctgatga aggaaagatc ttagatgtgg gatgcgggta
     7501 tggaccgatt gggttagctt tggcatttgc gacacagcgg tttgttgaaa tggtcgacat
     7561 taatgaacga gcggtaagtc tagcgcaagg aaacgcaaag cgtaaccaaa tcgaacatgt
     7621 tgatatccat caatcgaata tctatgaagc agttcatgaa gaaacctatg cagctatcgt
     7681 aagcaatccg ccgatccgtg ctggaaaaaa agttgtacat gaaattttga caggcgcata
     7741 tcctcggttg aagaagggcg gtacactgac aattgtgatc cagaagaaac aaggagcgcc
     7801 gagtgcgcaa aagaaaatgg aagaaacatt tggtaatgca gaaatcgtca caaaagataa
     7861 aggatattat atcatcaaaa gtgtgaaaga ataactagct gaaaaatagt ggagcgacga
     7921 aagagtggaa ctgggcgtgc atgtacgggt acgtatcagt tctttttttg ttgagaagtt
     7981 tttaaaaaca aagaactatt ttttcttcaa aaagaaatag taggaacaga gtcgttgttt
     8041 tttacgataa aataaataag atacagaact tgtatagaag gctgccaagt cggtagataa
     8101 aggaggtttt tatcaaaaaa catactttcc ttttttctct attcttttct tgggggtttt
     8161 ggtacactga tagggaattt agatagaagg gagccaatag gatgaagctg accatcagag
     8221 atatcgcaga aatggcaggc gtatcagtaa caactgtctc acagattttg aataacaaag
     8281 gaagcagatt tagtgataag acaagaaaaa aagtgttgtc gatcgtgaat gaacatcatt
     8341 ataagcccga ttatttcgct tcaaatgtga tcaatcgaca ttcaaagacc atcggaatga
     8401 tcgtccctga tgtcactgat ttcttcttct ccaaactgat cgaaggtgta gagagctatt
     8461 tgaatccttt gggctatgtg atcatgttat gcaattctcg tcatagccaa gaaaatgaga
     8521 tcaagtattt acaagagctt tcccaccgct cagtggacgg aatcttgctg gccacaccaa
     8581 atattttacc tgagaaatat gcattagaca gcggttttta tcaaaaaatg ccaatcattt
     8641 taatcgatcg cggattgaat cgtcgggata acggacggct tatcgtaaag gaatatgagg
     8701 gcgcctatca agctgtttcg ctgtttattg aaaatggcca tacgaagatc ggcatgctga
     8761 aggaaaccac tggatactat caattagaag aacgtttcaa cggatatcgt catgcgctga
     8821 aagatcatgg cctgcctttt aatgggaaat ttgttgaaca tggagagttg actgtccaag
     8881 gtggatatac tgcatcaaaa aaattactca aacataaaga tatcacggct attttttgcg
     8941 ggaatgatgc catggcgatc ggatgctatc aggcgatcga tgaattgggc aaaaaaatcc
     9001 cagaagatat ctcagtcata ggattcgatg gattaaagct gtcagaatac atgattccgc
     9061 aattgacgac agtgcagcag ccaagttttg atattggatt ttatgctgca cgtttcctga
     9121 tcgatacgat cgaatttcct caaagaaaag ttccgaacaa agtgttcgaa acaaaattaa
     9181 ttattcgtta gagcgtaaaa gtattgacaa agggctaact cggcggtata ttgcttatgt
     9241 aagcatttac aaaggctagt tagtgctttt tttattttta gtaaaccagt ttactaaagt
     9301 ggtttacctt tcctaaaaat aatgtataat accgaatgaa gacaaggagg atcaggcaat
     9361 gcgaatggtg gatctgattg aaaaaaagag ggatggaaac gagctgtcga aagaggaaat
     9421 cgagtacatc gtcacgaact atacgaatgg aaagattcca gattatcaag tcagtgcatt
     9481 attgatggca attttttatc aagacatgac aaatgaagaa atcacgaact taacattggc
     9541 gattgctaat tctggtgatg tcatcgattt gagttctctt gaaggaataa aggtagataa
     9601 gcattcgaca ggtggcgttg gtgacacgac cacattgatt ttagcccccc tagtagctag
     9661 tgtcggagtg acagtggcta aaatgtctgg acgcggtcta ggttacacag gtggtacact
     9721 agacaagcta gaagcaattc ccggatttca gatcgagctt tcggatgaag aatttgtacg
     9781 cattgttaat gaaagtaaag tagcggtaat tggtcaatca ggaaacttgg caccggcgga
     9841 caaaaaattg tatgccttgc gtgacgtcac agcgaccgtt gattctcttc cgctgattgc
     9901 cagttcgatt atgagtaaaa aaatcgcggc aggagcagat gcaatcgttt tggatgtgac
     9961 cactggagac ggtgctttca tgaagaacat cgaagatgca agacgtctgg caaagaccat
    10021 gacgagcatt gggaaactgg ctaaccgaga aacagtggcg gttatctcag atatgtcaga
    10081 acctcttggc gaagctatcg gaaatagtct agaagttgtc gaggcaatcg aaacattaca
    10141 aggaaatggt ccagaagatc tagtagaaat gtgctacgcc ttaggaagcc aaatggttgt
    10201 tcttgctggc aaagctaaga caaccgatga agcaagaacg ttgcttcaag aagctctgga
    10261 atcaggtaaa gcgttagcaa aattcaaaga aatgatccaa aatcaagggg gagacccagc
    10321 gatcgtggaa catccagaac gaattttgac tgcacgctac acaatggagc ttcctgctaa
    10381 acaaagcgga gttgtgtcaa aaatcgtggc aaatgaatta ggaattgctg caatgatgct
    10441 aggtgctggg cgtaaaacca aagaagaaga tattgatcac gctgttggat tgaaactgca
    10501 taaaaaaatt ggtgacactg tgacaaaagg tgagtcacta ttgacgatct atagtaacga
    10561 cgaagaaatc acttctgtca ttgaattatt atataaaaat attgaaattg gtgaatcggc
    10621 aatgaagcca acgttgattc atgacatcat cacagaataa aggaaaaaag cggactttac
    10681 cgttttaaag aaaaggggaa aagaaatgga actaaaccga atgatcgacc atactatttt
    10741 gaaagcagac gcatctaaag aagctgtgat gcaaattatc gaagaagcaa aaaaatacca
    10801 tttttattct gtctgcatca atccagcatg ggttgcgcta gcgaaagaac aattacaagg
    10861 tgagccagta gccgtatgta cagtaatcgg gtttcctcta ggagcaaaca cacccgagac
    10921 aaaagcattt gaaacaacag atgccatcaa taacggtgca gatgaagtcg atatggtcat
    10981 caatatcggt gctttgaaat caaaggatga tcaacaagtc caaaaagata tcgaagcagt
    11041 tgttgaagca gcaaaagatc gtgcgcttgt caaagttatt attgaaacat cgttattaga
    11101 tagagaagaa atcatccgag cttgcgaaat agcaaaagct gcaggcgctg attttgtcaa
    11161 aacatcaact ggtttctcaa caggcggagc aaaggtagag gatgtccgtt tgatgcgtga
    11221 aactgtagga ccagaaatgg gtgtcaaagc ttccggcggt atccataacg aagaagaagc
    11281 aatggcaatg atcgaagctg gtgctacaag aatcggaaca agcgcaggag tagcaatcgt
    11341 atctggttca actggtgaag gctattaatt ttttcaaagg agaagctcat ggcagaactc
    11401 aaacaagaat ggatcaacac agctattgaa gcgttagata aagcctatgt tccttattcc
    11461 cattttccgg taggcgcttg tctcgtaacg gaatcaggca agatttatca aggcatcaac
    11521 attgagaatg catcatttgg attaacgaat tgtgccgagc gcacagcttt tttcaaagca
    11581 gtatctgaag gagaaaaaac atttacgcat ctagtcgtag cggggcacac ccccgatcct
    11641 atctctcctt gtggtgcatg tcgacaagta atggcagaat tctgttcccc tgatatgccg
    11701 gttacgctag taggagacaa tggtgtgacg aaggcaacga ccgttcgtgc actgctgcct
    11761 tatgcattta ctgaaaaaga tctataactc atgttcattc agtcagctga ctgttgaaaa
    11821 ataaaacaat tattttagga ggctttacag aaatgaaaaa agcaaaatta tttggtttag
    11881 gtgctgtcgc actagcagca gggttatttt taggtgcatg cggcaacaac ggctctactg
    11941 attcatccgg tggcaaaagc tcatcagaca caacaactgc agcattgatc acagacacag
    12001 gtggtgtaga cgaccgttca ttcaaccagt cagcttggga aggactagaa aaatggggga
    12061 aagatcaagg actttcacga ggaaatgatg gcttccaata tttccaatct tcaaatgaat
    12121 cagattacat cccaaatatt gaccaagcat tgaatgctgg attcaaaacg atttttggta
    12181 ttggttataa actaaaacca gcgatccaag aacaagcaac aaacaataca ggtacgaact
    12241 ttgttattat cgatgatgtc atcgatggat tagataacgt tgtatctgca actttcaaag
    12301 acaacgaagc ttcttatctt gcaggtgttg cagcagctta tacgactgaa acaaacgtag
    12361 tcggctttat cggcggtgta aaaggtgaag taatcgatcg ttttgatgct ggattcaaag
    12421 ccggtgttga tgcaggtgcg aaagaattag gaaaagaaat caaagtattg aatcaatatg
    12481 ctggtgactt ctctgctcca gataaaggac gttcaattgc tcaaggtatg tatgcacaaa
    12541 acgcagatat catcttccat gcttcaggcg gtacaggtaa cggtgtattc caagaagcaa
    12601 aatcattgaa cgaatcaggc gacaaaaaag tttgggttat cggtgttgac cgcgaccaat
    12661 cagacgaagg cgaatacaca ttaaacggcg aaaagaaaaa cttcacattg acatctacac
    12721 tgaaagcagt tggaacagta gtagaagatt tggcacaaaa atcagcggac ggtaaattcc
    12781 ctggcggcga acacactgtt tatggattga aagaagacgg tgttggtcta acagaaggac
    12841 aactttctga cgaagcgaaa aaagcagttg atgaagcaaa agaaaaaatc atttcaggtg
    12901 acgtaaaagt tccagaaact cctgaagaaa actaaaacca tttctatagc cttgttagac
    12961 acatgtttga caaggctatg gtttgattag aacaagaatt gttcatttat ccaagctgcc
    13021 ttgtgacagt ttggataagg gaacaactcc ctgaagtaat gtttgtttca aaagaagaac
    13081 gctcactaag cgtgaatctt agtcaatcgt tgccctagat agaaatcgtt aggatgtgaa
    13141 aaaaaggtgg ttcaagaaaa tattgtcata gaaatgcaga atattaccaa agaatttgga
    13201 acattcaagg caaatgacaa catcaattta caagtaaagg caggagaaat tcatgcgttg
    13261 cttggagaaa acggtgctgg caaatctaca ttgatgaacg tgctttccgg attattagag
    13321 ccgacatcag ggaaaatttt gatgcgtggg aaagaagtaa agatcacaag tccgaccaaa
    13381 gccaaccaat tagggattgg gatggtccat cagcacttta tgcttgttga tgcctttact
    13441 gtaacagaaa acatcgtgtt gggaagcgag cctagtcgcg cagggatgct tgaccataaa
    13501 aaagcacgaa aagagatcca aaaagtttct gaacagtatg gattgtcagt caacccagat
    13561 gcttacgttc gtgatatttc agttggaatg gaacaacggg tagaaatttt aaaaacactt
    13621 taccgaggag cagatgtact gatttttgat gagccgacag ctgtattgac ccctcaagaa
    13681 attgatgaat taatcgtgat catgaaggaa ttagtcaaag aaggcaagtc gatcattttg
    13741 attacgcata agttagatga aatcaaagca gtagctgacc gctgtacagt tatccgccgt
    13801 ggaaaaggaa tcggtacagt caacgttaaa gacgtaacct cacaacaatt agccgatatg
    13861 atggttggaa gaacggtttc attcaaaacg atgaaaaaag aagcgaagcc tcaagaagtc
    13921 gttttgtcta ttgaaaatct agtggtgaaa gaaaatcgtg gattagaagc cgtgaaaaac
    13981 ttgaacttag aggttcgtgc tggcgaagta cttggtatcg ctggaatcga tggaaacggg
    14041 cagtcggagt tgatccaagc tttgactggt ttgcgaaagg cagaaagcgg acatatcaag
    14101 ctaaaagggg aagacatcac taataaaaaa ccccgaaaaa tcactgaaca tggtgtagga
    14161 cacgtgccag aagaccgtca taaatacggg ttagtcctag atatgacatt atctgaaaat
    14221 atcgcactac aaacctatca tcaaaaacct tacagtaaaa acggcatgct gaattattca
    14281 gtaatgaatg aacatgccag agaattgatc gaagaatatg atgttcgaac aacgaatgaa
    14341 cttgttcccg caaaagcttt atcaggcgga aatcagcaaa aagcaattat cgctcgtgaa
    14401 gtcgaccgag atcctgatct gttgattgtt gcaaatccaa ctcgtggact ggatgtaggt
    14461 gcgatcgaat ttattcataa acgtctgatc gaacaaaggg acaaatacaa agcagtgtta
    14521 ctgatcagtt tcgaattaga agaaatttta aatgtttcgg atcgtattgc tgttatccat
    14581 gaaggagaaa ttgtcggtat cgttgatccg aaagaaacat ctgaaaatga attaggtcta
    14641 ttgatggcgg gctatacctt agaagaagca cgaaaagagt tagaaaaggg ggctggcgca
    14701 catgaatgat cgcagcgaaa aattccgtaa tttattagtg cctgtacttt cagttgtttt
    14761 aggattgatc ttaggcgcga tattgatggc tgcttttggt tttcagccca tccaaggtta
    14821 ttcttctatg ctagatgcag cccttggttc gcaaagaggg ataggtgaaa cgttaaggca
    14881 agcaacgccg ttgattttta cagcattagg tttttctgta gctaactcag ctgggttttt
    14941 caatattggg ctttctggac aagcactttg cgggtggatc gtcagtgtct gggtggcact
    15001 tgcgtttcca gatctgccaa aagttgtttt gcttccgtta tgcgtgatct taggtgcctt
    15061 agctggtgcg atggcagcgg ctgttccagg tttacttcga gcattttttg gaacaagtga
    15121 agtgatcgta acaatcatga tgaactatat cttattatac gtaagcacat ttgctttaca
    15181 tgaagtgatg cctgaaagct atcgctcaag tttggactct tccaatatga tcagtcaaaa
    15241 tgcctcactg cggttaccat ggttaacaca agcttttggc ggctctcgcg taaactcagg
    15301 attattttta gcaatcatcg cgcttatttt cgtttggctg ctgatgaaga aaacgacttt
    15361 aggatttgaa atccgttctg ttggattgaa tccttttgct tctgagtatg caggaatgag
    15421 tagtaaacgt acgatcgttt tgtcgatggt catttctggc gctttggcag ggcttggcgg
    15481 tgtggttgaa ggattaggaa cgtatcaaaa cttctttgtt caaacgactt cgctatctat
    15541 tggatttgac ggaatggctg tttccttgct tggttctgga tcagcaatcg gtattctttt
    15601 atcagcgatc ttgttcagta ttttgaagat cggtggactt ggcatgcaga caggagcagg
    15661 tgttccgttt gagatcgtca atgtatcgat tgctttgatt atcttctttg tcgggatcaa
    15721 cttcctgatt cgtttcgtca tggctaaagt cttccaaggg aaaaaacaag aagagatcgt
    15781 tgcgacaata gaatcgaaac caacgaatca aaaccatgaa gggggcgaac tataatgtcg
    15841 actatcgaat taattgcctt gatccttact tctacactgg tttattcaac accgctaatt
    15901 ctaacgtcac taggcggaac tttttctgaa cgaagcggga tcgtaaacgt agggctagag
    15961 ggaatcatgg tcatgggtgc ttttagttcc atcgttttca acttgacgat gagtccatca
    16021 cttggttcta tgacaccatg gatcgctatt ttagtgggtg gcgtcgtagg tatcctattt
    16081 tctctgattc acgcagtagc aacagtcagt ttgcgtgctg accatattat cagcgggaca
    16141 gtaattaatc tgatggcacc tgctttaggt gttttcttga tcaaagcatg gtatggtaaa
    16201 ggtcaaactg ataacatcac gcagaatctg gggtattttt ctttcccagg attagcagat
    16261 attccggtca ttggaccaat tttctttaaa aatacattct tacctgctta ttgtgctatt
    16321 ttgattgctg tcctagcttg ggtgatcatc ttcaagacga aattcggctt gcgcctccgt
    16381 tcagttggtg aaaatcctca agctgccgac acattaggta tcaatgtata tgctatgcgt
    16441 tattcaggcg tactgatttc tggcttgtta ggcggtatgg gcggtgctgt ttttgctcag
    16501 tcgatttccg gtaacttttc agctggtacg atcgttggac aagggttcat ctccatggct
    16561 gctatgatct ttggtaagtg gaatccttta ggggcaatgg gtgcctcatt gttctttggt
    16621 tttgctcaaa gcttgagtat cattggcgca caattaccag ttatttcttc gattccgcca
    16681 gtattgctgc aaattgctcc atatttgctg acaatcattg tgctggttgt tttcttaggg
    16741 aaagcttctg gtccaaaagc aaacgggaag aactatatca aatcaaaata atcttttagc
    16801 gaataaaaag ggtgggttac gggaagtgtt tttcgtctcg ccctttttgt tttaaaggca
    16861 ataaaaataa aaagaaagaa ggaataatca tgtttaaacg cgtacatctg atcgtcatgg
    16921 attcagtagg aattggagaa gcaccagatg ccgataagtt cggtgatgtt ggttctgata
    16981 cattaggaca cattgcgaaa gaagcaggat tgaacgttcc acatttagag cagcttggtt
    17041 taggaacgat ccgtcctctt gaaggtgtgg aatcagtgaa agatcatcaa ggctatgcga
    17101 cgaaattaga agaagtttct gtagggaaag atacgatgac aggccactgg gaaatcatgg
    17161 gattgaatat caaaactcca tttcgtgtgt ttcctaatgg attccctgat gatctgatca
    17221 aacaaatcga agaattttct ggccgtaaag tcgtatgtaa caaaccttat agcggaacag
    17281 ctgttattga cgattacggt gaacaccaaa tgaaaacagg cgatttaatt gtatatactt
    17341 ctgccgatcc tgtccttcag atcgcggcac atgaagaaat cattccttta gaagaattat
    17401 atcggatttg ccaattcgtt cgtgatataa caaaagatga tccgtatatg attggccgaa
    17461 tcattgcacg tccgtacgtg ggtgaaccag gagacttcac acgaacaagc aatcgccatg
    17521 attatgcgtt gaatccattc ggacacacag tccttgattc tttgaaagaa gcaggaaagg
    17581 acgtgattgc tgttggaaag atcaacgata ttttcaacgg acaaggaatc actgaatctg
    17641 tccgcacgaa aagcaatatg gatggcgttg accagctgct gaaagttatg aaaaacgatt
    17701 tttcaggaat cagctttacc aatttagtag attttgatgc attatacggt catcgtcggg
    17761 atgtagtcgg ctatgcgcat gcgattgaag aatttgactt acgaattcca gaattgcttg
    17821 aagcgatgga agaagacgac ttgctaatga ttacagccga tcacggaaac gatccgacgt
    17881 ttactggtac cgatcatacg cgagaatatg taccgttatt ggtttacagt aaaaaaatga
    17941 aagaacaagg ggcattgcct caaggatatt attcagatat ttcagcaacg attgctgaga
    18001 actttagtgt ttcttcgact gaaaatggcg aaagcttctt gaaattatta aaataatctt
    18061 tttaaatagg agaaagcaaa tgaccaagtt aagcgagatg ttacaagaaa cgacccagtt
    18121 tatccttgat aaaggagtaa aagaaattga ttttggtttg attctaggtt caggattagg
    18181 tgaattagca gatgaaatcg aagatgctgt aatgatttct tatgatgaga tcccgttctt
    18241 tcctgtttcg acagtagttg ggcatgcagg aaagctagtt tatgggtctc tggcaggcaa
    18301 gaaagtgtta gccatgcaag ggagattcca ctactatgaa ggtcattcca tgcaaacagt
    18361 gacctatcca gtacgtgtga tggccggact aggggcccat tcagtcattg tgactaatgc
    18421 atgtggtggt gtcaacgaat catttgtacc aggagatttg atgctgatta ccgatcatat
    18481 caattttact ggtgaaaatc ctttgatcgg tccgaatgaa gatgcaatgg gccctagatt
    18541 tccagatatg agtcaagctt atacgcctga ctatcaagca acagcaaaag cagcagcaga
    18601 aaaactaggt ttggatctta aagaaggcgt atatatgggc tattctggac caacttatga
    18661 aacgcctgcg gagatccgaa tgtctcggac aataggagcc gatgcagtag ggatgtcgac
    18721 tgttccagaa gtcatcgtag cagctcacag cggattaaaa gtgttaggaa tctcttgtat
    18781 taccaactta gcagcaggta tgcaagcgaa tctgagccat gaagaagttg tagaaacaac
    18841 tcagagagtc aaacaatctt ttaaagcatt ggttaaagaa acattgacat tattgtaata
    18901 ggaggaacaa taaaatgagt gtgcatattg aagcgaaaga aggcgagatc gccgataaga
    18961 ttttacttcc gggagatcct ttgcgagcta aatatattgc ggagactttt ttggaagatc
    19021 cagtttgtta caatcaggta cgcggtatgt taggctttac cggcaaatat aaaggagaac
    19081 gtatttctgt tcaaggaaca ggaatgggaa tgccatcagc caccatctat gcccatgaat
    19141 tgatccagtc gtatggtgta aagaaattga ttcgagtagg tacatgcggt gcattgtcaa
    19201 aagacgtcca tgttcgtgat ttagtactag cacaaggtgc agcgaccagt tcttcgatga
    19261 tcgaaaaaaa tttccaagcc tttcattttc ctccaatcag tgatttcaat ctattactga
    19321 aagcatatga gatagctaaa gaaaagggct atacggttca tgtaggaaat gttctttcgg
    19381 aagattcttt ctacaaagat gatttgacag agacgttcca attagctgaa cttggcgttt
    19441 taggagtgga aatggaagca gcggctcttt attacttagg cgcgaaatac catgtgcaga
    19501 cattaagttt gatgaccgtt agcgaccatt tgatcaccgg tgaagaaaca acagctgcag
    19561 aacgtcaatc tacgttcaat gaaatgatcg aagtaggatt ggaaacagcg atagcagaat
    19621 aagaaggaaa tgaaaaagct gccctgcatt aagtaataag aacatccaag caaaaatagc
    19681 tcctcatgtt tttgacttgg ctggacttat tattgcaaat gtaagctttt gaacaccgtt
    19741 taccaagagg ttgtgaatct gccgtttaac tctgagtcac aaggaacaat ttcaaaaaac
    19801 agcttttcat gtttgatgaa tattgtgact tgtgaccgag aagttggcag gagaaaaccg
    19861 tttatacgaa aaaagggact gtgacaaatc gtgtcacagt ccctttttta tagtgttttt
    19921 tataggtagt attttttagc aactgttaag tcatcattta attcataaac aagtggttga
    19981 cctgttggaa tttcaagatc catgatgtct tcgtcagaga taccttcgat atgtttagct
    20041 agggcgcgta atgagttacc atgtgcagct actaaaacag ttttgttgtc tagtaatgct
    20101 ggagcaattt catcttgcca gaatggcaaa gcgcgttcta atgtcacttt caagttttct
    20161 ccaccaggaa tatcgcgttt gtctaacatt gcgtaacgac ggtcatttgc tgcagaacct
    20221 tcgtcagttt cttccattaa tggaggaaga gtatcgtaag aacgacgcca gatgtgcact
    20281 tgttcgtcgc cgtatttttc agctgtttct tttttgttca aaccttgtaa tttaccatag
    20341 tgacgttcgt ttaagcgcca tgatttgatt tgtggtaccc aaagttgatc tgaatattct
    20401 aagatcaagt tacatgtttt gattgcacga gttaaaacag aagtgtaagc aacatcaaat
    20461 tcgattccag cttctttgat tttgcgtccg ccttcttttg cttcttcgat tccttctggt
    20521 gctagattta catcagccca accagtaaat tggttcaatg cattccattc actaagaccg
    20581 tgacgagaaa aaactaattt tggcatgttt agctctcctt ttaagatagg tttatatttc
    20641 tgaaattttc agttctgttt tattgtacaa ttttttggaa gagatttcca tcgaaaaatt
    20701 agataagatt ctatgagaaa acaagtagag aatataaaaa aacgaacagt gcccacgcaa
    20761 ctgttcgttt ttaggtatta gatgcataat tgctcgattt ctttttcagg taaattgata
    20821 tatttataac atgttccaac tgaaacaccg cattttgaag aaataaattg gatcgtttct
    20881 tttttttcat aatagagacg tcggattttt ttgatcgttt ttgcatcgat ttttggtctt
    20941 ccgccgattt ttcctttttt acgagcttca tctaatccaa ctaatgtacg ttctttgatc
    21001 aatgcgcact ccatgtttgc taggttttcc attaattgga aatagatttt tcccatttcg
    21061 cgcgtatcga ttccttcatc aagactaatc aagtgcagcc cgcgatcttt gaataattgc
    21121 gtgagttctg ttaattgccg cgtagatttg cctaatcgat gaagttggca aatgactagt
    21181 gcatcgcctt gttgcatgtc agcgatcact gactccaaat gaacagcagt agaatcattt
    21241 tgttccactt catatgtctc atggaaaatt tcttgacaac caaagtcaga taattgatca
    21301 acctgagttg ttaaatcaac gtcatttatg gttgtgcgtg cataaccaat aattttcatg
    21361 gtgattcctt tctagcagtg attttctaac tggatataac agtaaaaatg tagcataaag
    21421 tacgtcaaaa agatagtttt ttcgcaaatt ttccgctgtc tttttttatt ggatgatcaa
    21481 gcagtttttc tttatttacg acggattgcg tcaagaatct caacgccttt ttctgagccg
    21541 acaactactt tagaaaccat gtttaggaaa agcccatgtt ctactacccc aacaagttca
    21601 tctaaatagg tagctaagct ttctggctgt tcgatgactt ctagatgcag atcaatgatg
    21661 taatgaccac catctgtggt taagacttct ccgttttcgt ctgtacgcaa gacgggttgg
    21721 aatctttttt cttcaaaaat atgaagtagc tgttgggagc cataagggat cacttcaact
    21781 gggagtggga acttcccaag tttatcgact aatttagaag aatcaacgat ccaaatcgtt
    21841 tcttttgagt acgtagcgac tattttctcg aaaagcaaag cagcgccgcc gcctttgatc
    21901 ccttgaaaat cagcactgat ttcatcagca ccgtctattg tcaaatcgac tacggggact
    21961 tcatcaatac tctttaacgg tattcctaat ttttccgctt gttcttttgt cgcatttgaa
    22021 gtagtaactc ctgtgatacg taaaccttct tcacgcatac ggcgaccaat ctcttctacc
    22081 atataatagg cggttgaacc ggtcccgaga cctacgatca tcccgtcctt tacgaatttc
    22141 gcggcttcaa tgccagctaa ttgttttaaa ttcatctttg cgtccacctt tttttagttt
    22201 gagcatcgtg ctttcctcac tattttagcg tgatttacgg taaaaagata ccattctttt
    22261 ttattccaat aaagcttttt caaaaaaaat tgcatctctt gttgacatgt tttttcattc
    22321 gtgatattct aacaaaggtg ctttatctac aggtttctgt gaagaaacgt gctgactgtt
    22381 tatcaaagca tatttgataa ggatgcagac attgcattta ttttttatca caaaaaaaga
    22441 accacctgga tgtgtggaac tagacgcaaa tcgtgggaag gaggaaacaa ggaatgccta
    22501 caattaacca attagtacgt aaacctcgta aatcaaaggt ggaaaaatct aattcacctg
    22561 ctttgaacaa aggatataac agctttaaga aaactcaaac taacgtaaac tcacctcaaa
    22621 aacgtggggt agcaactcgt gtgggtacaa tgacacctaa aaaaccgaac tcagctttac
    22681 gtaaatatgc acgtgttcgt ttgtcaaact taattgaagt tactgcttat atcccaggga
    22741 ttggtcataa cttacaagaa cacagtgttg tattattacg cggtggtcgt gtaaaagact
    22801 taccaggggt acgttaccat atcgttcgcg gtgctttaga tactgctggt gttaacgacc
    22861 gtaaacaaag ccgttctaaa tacggaacta aaaaacctaa agcttaattt taattattaa
    22921 acaaatgaaa cttgcttcta ctatatagaa gtaattacta tgaacaatat ttcggaagga
    22981 ggagttacgg atgccacgta aaggtcctgt tgcaaaacgt gatgttttac cagatcctat
    23041 ttataactca aaattagtaa ctcgcttgat caaccgtgta atggttgacg gaaaacgcgg
    23101 gattgcagct aacattatct ataatgcctt tgacttgatc aaagaatcta caggtaacga
    23161 tccattagaa gtgtttgaac aagcaatgaa aaacgttcaa cctgttcttg aagttaaagc
    23221 acgtcgtgtt gggggttcaa actaccaagt accagttgaa gttcgccctg aacgccgcgt
    23281 tactctaggt cttcgttggg tagtaaacta cgctcgcttg cgcggtgaac acacaatgga
    23341 agaacgctta gcaaaagaaa tcatggatgc tgcaaacaac acaggtgctt ctgttaaaaa
    23401 acgtgaagac actcataaaa tggctgatgc caaccgtgcg tttgctcact atcgctggta
    23461 agattctctc tgtttggtcg gcttttataa gtcgacctca gatagtgtcc ttatttttag
    23521 caactagata gtaaagagag gagaaacaag gagaatgacc agagaatttt ctttacaaaa
    23581 cactcgtaat atcggtatca tggcccacat cgatgctggt aaaacgactg ctactgaacg
    23641 tatcttgtac tatactggta aaatccataa aattggtgaa actcatgaag gagcttcaca
    23701 aatggactgg atggaacaag aacaagaacg tggtattacg atcacttctg ctgctactac
    23761 agcacaatgg aagggtcacc gaatcaacat catcgacaca ccaggacacg tagacttcac
    23821 agttgaagtt gaacgttcat tgcgtgtact tgatggtgct gtaactgttc ttgatgcaca
    23881 atcaggtgtt gaacctcaaa ctgaaacagt ttggcgtcaa gcaacaactt atggtgtacc
    23941 tcgtatcgta tttgcaaaca aaatggacaa gatcggtgct gacttcttat attcagtatc
    24001 aactttacat gaccgtttgc aagcaaacgc tcacccaatc caattaccaa ttggtgcgga
    24061 agacgacttc acaggaatta tcgaccttgt aacaatgaag gctgaaatgt atacaaacga
    24121 tcttggaaca gagatcgaag aaacagaaat tcctgaagaa taccgcgaat tagctgaaga
    24181 atggcgcgaa aaattggttg aagctgtagc tgaaactgat gaagaactta ctttgaaata
    24241 cttagaaggt gaagaaatca ctgaagctga gttaaaagaa ggtatccgcc gcgcaacagt
    24301 taacgtagaa ttctacccag tactatgtgg ttcagcattt aagaacaaag gggttcaatt
    24361 actattggac gctgttcttg attacttgcc atcaccgtta gatatcccag ctatcaaagg
    24421 gattgaccct aaaactgatg aagaagtaga acgtcctgca gacgacagcg ctcctttctc
    24481 agctttagca tttaaagtta tgactgaccc attcgtaggt cgtttaactt tcttccgtgt
    24541 gtactctggt gttttacaat caggttcata cgtacaaaac gcaacaaaag gcaaacgtga
    24601 acgtgttggt cgtatcctac agatgcatgc caactctcgt tcagaaatct ctgaagttta
    24661 cgctggagat atcgctgctg ctgttggttt gaaagacaca actactgggg acacactatg
    24721 tgatgaaaag agtctagtta ttcttgaatc catggaattc ccagaaccag ttatccaagt
    24781 cgctattgaa cctaaatcaa aagctgacca agataaaatg ggtgtggctt tacaaaaact
    24841 atctgaagaa gatcctactt tccgtgctga aacaaacgtg gaaacaggcg aaacaatcat
    24901 cgctggtatg ggtgaacttc acttagatat catcgttgac cgtatgcgtc gtgaattcaa
    24961 agttgaagct aacgtaggtg cgcctcaagt atcttaccgt gaaactttcc gtgctggcac
    25021 tcaagccgaa ggtaagtttg tacgtcagtc tggtggtaaa ggtcaatacg gtcacgtatg
    25081 gattgaattt acaccaaatg aagaaggtgc tggtttcgaa tttgaaaatg cgatcgtcgg
    25141 tggggttgtt cctcgtgaat acattccagc tgttgaaact ggtttgaaag atgctatgga
    25201 aaatggtgtc ttagctggct atccattagt agatatcaaa gctaaacttt acgatggttc
    25261 ataccatgat gtcgattcaa atgaaactgc cttccgtgtt gctgcttcta tggcattacg
    25321 tgcagcagct aagaaagcta atccagtaat ccttgaacca atcatggctg tagaagttgt
    25381 tatccctgaa gattatttag gtgatgttat gggtcacgta actgctcgtc gtggacgtgt
    25441 tgaaggtatg gaagctcgcg ctggcggcca acaagttgtt cgtgcgatgg ttccacttgc
    25501 tgaaatgttt ggttatgcta caactcttcg ttctgctact caaggtcgtg gtacatttac
    25561 aatgacattt gaccactacg aagatgtacc aaaatcagta caagaagaaa tcattaagaa
    25621 aaacggcgga aaagctgaat aatttcttaa aaccggatag tcctgcaaag agattatcca
    25681 aagagactat atttttaata tagtttcgtg taaaataatt atcgatgata tgggcatgtg
    25741 gtgtaccttg tgtacatcat atacctatca gacaaataaa aaatatattg tatttttagg
    25801 aggacatttt aaaatggcaa aagaaaaatt tgaccgttct aaaccacacg ttaacattgg
    25861 tactatcgga cacgttgacc atggtaaaac tacattaaca gctgcaatca caactgtact
    25921 atctaagaaa aacggcggcc aagctatggc ttacgatcaa atcgatggtg ctccagaaga
    25981 acgcgaacgc ggaatcacaa tctcaacagc tcacgttgag tatgaaacag atacacgcca
    26041 ctacgctcac gttgactgcc caggacacgc ggattacgtt aaaaacatga tcactggtgc
    26101 tgctcaaatg gacggagcta tcttggtagt ttctgctgct gacggcccaa tgcctcaaac
    26161 tcgtgaacac atcctattgt ctcgtcaagt tggtgttcct tacatcgttg tattcttgaa
    26221 caaagtagac atggttgatg acgaagaatt actagaatta gttgaaatgg aagttcgtga
    26281 cctattaaca gaatacgaat tccctggtga cgatgttcct gtagttgctg gatcagcttt
    26341 gaaagctcta gaaggcgacg cttcatacga agaaaaaatt cttgaattaa tggctgcagt
    26401 tgacgaatac atcccaactc cagaacgtga caacgacaaa ccattcatga tgccagttga
    26461 agacgtgttc tcaattactg gacgtggtac tgttgctaca ggtcgtgttg aacgtggaca
    26521 agttcgcgtt ggtgacgaag ttgaagttgt tggtattgct gaagaaactt caaaaacaac
    26581 agttactggt gttgaaatgt tccgtaaatt gttagactac gctgaagctg gagacaacat
    26641 tggtgcttta ctacgtggtg ttgcacgtga agacatccaa cgtggacaag ttttagctaa
    26701 accaggtaca atcacacctc atacaaaatt ctctgcagaa gtatacgtgt tgacaaaaga
    26761 agaaggtgga cgtcatactc cattcttcac taactaccgt ccacaattct acttccgtac
    26821 aactgacgta acaggtgttg ttgaattacc agaaggaact gaaatggtta tgcctggcga
    26881 caacgtaaca atggaagttg aattaatcca cccaatcgct atcgaaaacg gtactaaatt
    26941 ctcaatccgt gaaggcggac gtacagttgg tgccggtgtc gttacagaaa tcaaagctta
    27001 attcacttaa gttttaaagt aaagcaacat ttattcggac gtaatcgctc ggaacgaagg
    27061 tcttaggatc tttgttccga gcttttttat ttattgcagg atcaggaaaa aagacaggag
    27121 tattataaat agaagaaaca cgaaataaaa gagtcagtat tttctaaatg aaactttatg
    27181 aaaacaaaat gttttttaca agaaaaagcc ttgctttgtg agtttatata tattataatg
    27241 acaaaggtgc gtttacttta gtgaattgca cagacgtaat cagaatgatt tcgtcggcta
    27301 agaaacgaga ggttgcgaca cgcccggacg ctttgccacg aacgagcgtg acggaaattt
    27361 tcgtggagct atgtctactt ttcaaaatag gcgaaggagg gaacaagatg gcaaaacaaa
    27421 aaattcgtat ccgtttaaaa gcgtatgaac accgtatttt agatcaatca gcggataaaa
    27481 tcgtggaaac tgcaaaaaga actggagcta gcgtgtctgg tccaattcca ttaccaactg
    27541 aacgcagtct ttacacaatc atccgtgcga ctcataaata caaagattca cgcgaacaat
    27601 tcgaaatgcg tacacacaaa cgtcttatcg acattgtgaa cccaacacca aaaactgttg
    27661 atgctttgat gaagctagac ttaccatctg gtgtaaacat cgaaatcaaa ttataaaact
    27721 aaaacaaaaa taatggaggt gtactcatga ccaaaggaat cttagggaaa aaagtgggaa
    27781 tgacacaaat cttcactgaa tctggtgaat taattccagt aactgttgta gaagctactc
    27841 caaacgtagt actacaagtg aaaacaatgg aaacggatgg ctacgaagct attcaagttg
    27901 gttaccaaga tatgcgtgaa gttttatcaa acaaacctgc gaaaggtcat gttgcaaaag
    27961 caaacacggc tcctaagcgc ttcattcgtg aattcaaaaa tgttgagcta ggagaatatg
    28021 aagtaggaaa agaaattaca gtagatgttt tccaagcagg agacattatt gatgtaacag
    28081 gtactacgaa aggtaaagga ttccaagggg ttatcaaacg ccacggacaa agccgcggac
    28141 caatggctca cggttctcgt caccatcgtc gtcctgggtc aatgggtcca gttgcaccta
    28201 accgtgtatt taaaaacaaa cgtcttgctg gccgtatggg tggcaaccgc gtaactatcc
    28261 aaaacttgga agttgtacgt gtcgatgcag aaagaaatgt tatcttgatc aaaggtaatg
    28321 ttcctggagc taaaaaatct ttaattacta tcaaatcagc tgtgaaagct aaataattgt
    28381 aggaagaaag gaggaactaa ggaatgccga atgtagcatt attcaaacaa gatggaagcc
    28441 aaaacggtga aatcacactt aacgaagaaa tcttcggaat tgaacctaat gaaagtgttg
    28501 tttacgatgc aatcgtaatg caacgtgcgt cattaagaca aggaacacac gcggttaaaa
    28561 accgtagcgc tgttcgcggc ggcggacgta aaccatggcg tcaaaaagga acaggtcgtg
    28621 cacgtcaagg atctatccgc tcaccacaat ggcgtggagg tggcgtagtc tttggaccaa
    28681 caccacgttc ttacagctac aaacttccta aaaaagttcg tcgtttagca atgaaatctg
    28741 tattgtcaga aaaagttgct gaaaacaact tggtagcaat cgaaggcttg aacttcgacg
    28801 caccaaaaac aaaagaattc aaacaagttc ttgctaactt atctatcgac tctaaagtat
    28861 tggttgttct agaaaaaggt aacgactttg ctgctttatc agcacgtaac ttaccaaacg
    28921 tttctgtagt aacttctgat aatgtgagtg tgctagacgt tgtatcaaat acaaaagtgt
    28981 tagcaacaca aactgctctt actcaaattg aggaggtgct tgcataatga acttactaga
    29041 cgtaatcaaa cgcccagtga tcactgaaaa atcaatgctt gccatggatg acaagaaata
    29101 cacttttgaa gtggacactc gcgcaaacaa aactttagta aaacaagcgg tagaagctgc
    29161 ttttgacgtt aaagtcaaaa acgtgaacat cgtgaacgta cgtgcgaaat tcaaacgtat
    29221 gggcaaatat gcaggatata caaaaaaacg tcgcaaagcg atcgttacat tgactgaaga
    29281 ttcaaaagaa atccaattat tcgaagctgc tgaataagca ctaatagata actcattaaa
    29341 ttaggaggga aaaacgtggc gattaaaaag tacaaaccta cctcaaatgg tcgtcgtaac
    29401 atgacaggtt ctgattttgc tgaaatcaca acatcaacac ctgaaaaatc attgttacaa
    29461 ccattgaaaa accatgctgg acgtaacaac aacggccgca tcactgtacg tcatcaaggt
    29521 ggcggacaca aacgccaata tcgcgtgatc gacttcaaac gtaacaaaga taacgttgtt
    29581 gcaactgttc aaacgatcga atatgatcca aaccgttcag ctaacatcgc gttagtacac
    29641 tatgaagatg gagtgaaagc ttacatctta gcacctaaag gactagaagt gggtatgaaa
    29701 cttgtttcag gtccagaagc agatatcaaa gtcggaaatg ctcttccatt agaaaacatt
    29761 ccagttggta ctgttatcca caacattgaa atgaaacctg gtaaaggtgg acaattgatc
    29821 cgttctgctg gtacaagtgc tcaagtactt ggtaaagaag gcaaatacgt attgatccgc
    29881 ttgaactctg gtgaagttcg tatgatctta gctgcatgcc gtgctacaat cggttctgta
    29941 ggtaacgaac aacacgaatt aatcaacatc ggtaaagctg gacgctctcg ttggatgcgt
    30001 aaacgcccaa ctgtacgtgg tagcgtaatg aaccctaatg atcacccaca cggtggtggt
    30061 gaaggtaaag ctcctatcgg acgtaaagca ccagtttctc cttggggtca accagctctt
    30121 ggttacaaaa ctcgtaacaa aaaagctaaa tcagacaaac ttattgttcg tcgtcgcaaa
    30181 aataaataat tactttgacc atgtgtcgca cattttgaga ggaggttcac catgggtcgt
    30241 agtttaaaga aaggaccttt tgtcgatgag catttaatga aaaaagttga agctcaagca
    30301 ggggttgaaa agaaaaaagt tatcaaaact tggtcacgcc gttcaacagt tttcccaagt
    30361 tttgttggat ataccatcgc agtttatgat ggacgtaaac atgtaccagt ttacatccaa
    30421 gaagacatgg taggacacaa attaggtgaa tttgcaccta caagaactta tcgtggacat
    30481 gctgctgacg ataagaaaac gaaacgctaa tttgagggga ggataaatcc aaatggcaga
    30541 acaaatcaca tcagctaaag caactgcaaa aacagttcgc acttcacctc gaaaagcccg
    30601 tttagtaatt gaccttatca gaggtaaaag tgtcgcagat gcaatttcaa tcttgaaatt
    30661 cacaccaaac aaatctgctg gaatcattga aaaagtattg atgtcagcag ttgcgaatgc
    30721 agaaaataac tttgacttag atgttgaaaa cttggtagta agcgaagctt ttgttaacga
    30781 aggacctaca atgaaacgtt tccgtcctcg tgcaaaagga tcagcttctc caatcaacaa
    30841 acgtacaagt catatcacgg tagtcgtatc agaaaaataa ggagggataa tcagtgggtc
    30901 aaaaagtaca tccaattgga atgcgtgtag gcatcatccg cgactgggat gctaaatggt
    30961 atgctgaaaa agagtatgct gagttcttac acgaagattt aagaatccgt aaatttattt
    31021 caactaaact tgctgatgct gctgtatcaa cagttgaaat cgaacgcgca acaggtcgcg
    31081 taaacatttc aattcacaca gctaaaccag gtatggttat tggtaaaggc ggttctgaag
    31141 tcgaaagcct aagaaaagaa ttaaacaaat taactggtaa aagaatccat atcaatatcg
    31201 tggaaatcaa aaaaccagat ctagatgcaa aattagtagg tgaaggaatc gcacgtcaat
    31261 tagaaaaccg tgttgctttc cgtcgcgctc aaaaacaagc catccaacgc tcaatgcgtg
    31321 ctggcgctca agggatcaaa actcaagtat ctggtcgtct aaacggagca gatatcgctc
    31381 gttcagaagg atactcagaa ggaactgttc ctcttcatac tttacgtgcg gacattgatt
    31441 acgcatggga agaagcagat acaacatacg gaaaactagg agttaaagtg tggatctatc
    31501 gtggagaaat tcttccaact aaaaaaaaca ctgagaaagg agggaaataa tcatgttagt
    31561 acctaaacgt gtaaaacacc gtcgtgaatt ccgcggaaaa atgcgcgggg aagctaaagg
    31621 cggaaaagaa gtagcattcg gtgaatacgg tttgcaagct gttgattcac attggatcac
    31681 aaaccgccaa atcgaagctg ctcgtatcgc aatgactcgt tacatgaaac gtggtgggaa
    31741 agtatggatt aaaattttcc ctcacaaatc ttatactgcc aaagcaattg gggtacgtat
    31801 gggttctggt aaaggggcac ctgaaggatg ggttgcacca gtaaaacgtg gtaaaatcat
    31861 gtttgaaatc gcaggcgttc ctgaagaagt agctcgtgaa gcgttacgtc tagcttctca
    31921 caaattacca atgaaaacta agatcgtaaa acgtgaggaa atgggtggtg aatcgaatga
    31981 aggttaaaga aatcagagaa ttaaccactg ccgaaatgct tgatcaagaa aaacaattaa
    32041 aagaagaatt gtttaatctt agattccaat tagcaacagg tcaattagaa aacactgcac
    32101 gtattaaaga agtacgtaaa tcgattgcac gcatcaaaac tgtgttgcgt gaacaagcta
    32161 agtaattatg gaaggaggcc attaagcgta tgactgaagg aagaaatcaa cgcaaagttt
    32221 atcaaggtcg cgtggtttca gacaaaatgg ataaaacaat taccgttgct gtcgaaacaa
    32281 agaaaaacca ccctatctat ggcaaacgca tgaactattc taagaaatac aaagtgcatg
    32341 atgaaaacaa cacagcaaaa gttggcgaca tcgtgagaat tatggaaact cgtccattat
    32401 cagctacaaa acgtttccgt ttgttggaag tagtcgaaga agcagttatt atctaataac
    32461 acgagatttt cctatataga ttgaatgcaa aatctgaaag gaggatacac aacgtgatcc
    32521 aagcagaaag tcgtttaaaa gtcgctgata actcaggcgc acgtgaaatt ttgacgatca
    32581 aagtccttgg tggatctggt cgtaaaactg ctaacatcgg tgatgtgatc gttgctacgg
    32641 ttaaacaagc aacgccaggt ggggttgtca aaaaaggtga cgtcgttaaa gccgttatcg
    32701 ttcgaacaaa atcaggagct cgtcgtacag acggttcata tattaaattt gatgaaaatg
    32761 ctgcagtgat tattcgtgat gataaaagcc ctcgtggaac acgtatcttt gggccagttg
    32821 cacgtgaact acgtgaaaac aacttcatga agatcgtttc actagcacca gaagttttat
    32881 aatcttgaga cacttatcaa aggaggtgcg aaacagttat gtttgttaaa aaaggcgata
    32941 aagttaaagt tatcactgga aaagacaaaa acaaagaagg cgttgtacta gcagcgttcc
    33001 cgaaaaaaga taaagttatt gtcgaaggtg taaatgtcgt taaaaaacac caaaaacctt
    33061 ctcaagctgc tcctcaaggt ggaattgttg agatggaagc gccaattcat gtttctaacg
    33121 tgatggtggt tgactctact ggtgtagctg gccgagttgg ctacaaagaa gtagacggca
    33181 aaaaagtccg tgtttctaaa aaaaccggtg aagttttaga taaataaaag tttaaggaag
    33241 gagggtctta ctgaatgaac cgcctaaaag aaaaatatct taaagaagta actccatcat
    33301 tgatggaaaa atttaactat agctctgtta tgcaaacacc taaagttgaa aaaatcgtca
    33361 tcaacatggg tgttggtgat gcggtatcaa acgctaaaaa cttagataaa gcagttgaag
    33421 aattaacttt gatcactggt caaaaaccaa tgattacaaa agctaaaaaa tcaatcgctg
    33481 gattccgttt acgtgaagga atgccaattg gtgcaaaagt tacattgcgc ggagaaagaa
    33541 tgtacgaatt cttagacaaa ttggtttctg tttcactacc acgtgtacgt gacttccatg
    33601 gtgtaagtaa aaaagccttt gacggtcgtg gaaactatac tttaggtatt aaagaacaat
    33661 tgatcttccc agaagtagat tatgatttag tagataaagt acgtggtatg gacatcgtta
    33721 ttgtaacaac agcaaactct gatgaagaat ctcgtgagtt gttgacacaa ttaggcatgc
    33781 cattccaaaa ataattaaag gaggcgaact acgtggctaa aaaatcaatg attgctaaaa
    33841 acaaacgtcc tgcaaaattt tcaacacaag cttatactcg ttgcgaacgt tgcggacgtc
    33901 cacattcagt ttatcgtaag ttccatcttt gccgtatttg cttccgcgaa cttgcctata
    33961 aaggtcaaat tcccggcgtg aagaaagcta gctggtaaaa agtaaattcg cataagaagg
    34021 aggtaaaaga tcaatggtca tgacagatcc aattgcagat tttctaactc gtatccgtaa
    34081 cgcaaacatg gttaaacatg agtctttaga agtacctgca tcaaaaatta aacgtgacat
    34141 cgctgaaatc ttgaaacgtg aaggtttcgt tcgtgatgtt gaatacatcg aagatgacaa
    34201 acaaggcgtg atccgtgttt tcctaaaatt tggtaaaaac ggcgaacgtg ttatcacaaa
    34261 cttaaaacgt atctctaaac caggtttgcg tgcttatgtc aaagctgacg aagtaccaaa
    34321 agtattgaac ggtttaggta tcgcaatcat ttcgacttct gaaggtgttg tcactgataa
    34381 agaagctcgt gaaaaaaata tcggcggcga agttatcgct tacgtatggt aatcaagtag
    34441 aatctacaag gaggtgtctt taagtgagcc gtatcggaaa taaagtcgtt gttatccctg
    34501 aaggagtaac agttactcaa gacggaaaca acgttacagt taaaggacca aaaggtgaat
    34561 tgactcgtac tttttcagca gatatcaaaa tgaatatcga aggaaacgaa gtaactttca
    34621 ctcgtccaaa cgacagtaaa gaaatgaaaa caattcatgg aacaactcgt gcgaacttca
    34681 acaacatggt tgtaggtgtc agcgaaggtt tccaaaaagc gttagaacta atcggggttg
    34741 ggtaccgtgc acaattacaa ggatcaaaac ttgtattgaa cgttggttac tcacatccag
    34801 tagaaatcac accaccagct ggagtaactg ttgaagtacc agctaacaca caagtaattg
    34861 ttaaaggtgc gaacaaagaa gaagttggcg aattagctgc aaacattcgt ggaactcgtc
    34921 ctccagaacc ttataaaggt aaaggtattc gttatgttgg cgaatatgta cgccgtaaag
    34981 aaggtaaaac tggtaaataa tcgtttagaa aaattgcata gaagccagca gcaggaaaaa
    35041 ggcatttggc tgaagtttac cttcaaaaaa atacattttt acttgtgccg cagcttctat
    35101 ttttcgaaac gtatttactg cttgaaatag tagtacaata aaaataaaga ggtgacaatt
    35161 gtgattacaa aaccagataa gaacaaaaca cgtcaaaaga gacatcgtcg tgtacgtaac
    35221 aatatctctg gtactgctga gcgcccacgc ttgaacgttt ttcgttctaa caaaaacatc
    35281 tacgctcaaa ttattgatga cgtagcgggt gtaacgctag caagtgcctc taccttggat
    35341 aaagaaattt caggtggaac aaaaacagaa gcagcacaag ctgtcggtaa actagttgct
    35401 gaacgtgcag ctgaaaaagg cattaaagta gtagtctttg accgtggtgg atacctttac
    35461 catggccgtg tgcaagcttt agctgaagct gctcgcgaaa atggactaga attttagaag
    35521 aaggaggaat accattcatg gtttatatcg atccaaaaca tttggaatta gaagaccgcg
    35581 ttgttgcaat taaccgtgta acaaaagttg ttaaaggtgg acgtcgtcta cgttttgctg
    35641 ctttagttgt tgtcggtgac aaaaacggac acgtagggtt tggtacaggt aaagctcaag
    35701 aagttcctga agcaatccgt aaagcaattg aagatgcgaa gaaaaactta gttgaagtac
    35761 ctatggttgg ttcaacaatc ccacacgaag taatcggtgc ttttagtggt ggtagaatcc
    35821 taatgaaacc tgctgtagaa ggttctgggg ttgctgctgg tggaccagtt cgtgccgtat
    35881 tagaattagc tggggtagca gatatcactt ctaaatcttt aggctcaaat acaccaatca
    35941 acgttgttcg cgcaacagtt gaaggtttga aacaattgaa acgtgcagaa gaagttgcag
    36001 aacttcgcgg caaatcagtt gaagaattaa tcggataagg aggacaacaa taatggctga
    36061 attaaaagtt actttaaaac gcagtattat cggacgtcct caaaaccaac gcgatacagt
    36121 taaagcgtta ggtcttacaa agattaacag ttcagtggtt aagccttcta atgaagcaat
    36181 taaaggcatg atcaatactg tttctcacct agtggacgtt gaagaaattt aatttagaaa
    36241 aatcataact agaacattaa ggaggtgcca aacctatgaa acttcatgaa ttaaaacctg
    36301 ctgaaggttc acgccaagta cgtaaccgtg ttggtcgtgg tacttcatct ggtaacggaa
    36361 aaactgccgg acgcggacaa aaaggacaaa aagctcgttc aggcggcggt gtgcgtttag
    36421 gatttgaagg gggacaaaca ccattgttcc gtcgtttacc aaaacgagga ttcacaaacg
    36481 tgaaccgtaa agactatgca gtcattaatc ttgatgtatt gaaccgcttt gaagacggag
    36541 ctgaagtaac tcctgttgct ttagttgaag ctggaatcgt gaaaaacgaa aaagctggaa
    36601 tcaaagtatt agctaatggg gaactaaaca agaaattaac tgtgaaagca gcaaaattct
    36661 ctaaagcagc tcaagaagca attgaagcgg ctggtggatc aatcgaggtg atctaatgtt
    36721 tagactatta aaagatgctt ttaaagtaaa agacattaga tcgaaaatcc tatttactgt
    36781 atttgtgttg tttgtatttc gcctaggtgc acatattact gtaccaggcg taaatgctgc
    36841 ttcattgcaa aacctttctt ctttgccatt tttcaacatg ttgaacttgg taagtggtag
    36901 tgcgatgcaa aacttttcca ttttctcaat gggggtttca ccatacatta ctgcttctat
    36961 cgtcattcag cttcttcaaa tggacatcgt gcctcgattt gttgaatggt cgaaacaagg
    37021 ggaagttggt cgtaaaaaac tgaatcaagc gactcgatat ctgacattag tattggcatt
    37081 tatccaatct gtcggtatca ctgctggatt caatcaatac gctcaattag gtttcgtgaa
    37141 taatcctaac gtagcaactt acgtaacgat cggtttgatt ctaacagccg gtacgatgtt
    37201 tgttacttgg ctaggagaac agatcacaga aaaaggtatc ggaaatggcg tatcaatgat
    37261 catttttgca ggtatcattt ctagattgcc tgaaagtatc agagaaattt atgaagatta
    37321 ctttataaat attgatcctt cagacatatg gaaatctgct atttttgtgg ccattttact
    37381 agtagcggtc ttgttgatcg tgacattcgt aacatttttc caacaagctg aacgcaagat
    37441 cccgatccaa tatacgaaac gcgtagctgg cgcgcctaca agcagctatc tgccgttgaa
    37501 agtaaatgca gctggagtta ttccagttat ctttgctagt tcgtttattg ctacgccaaa
    37561 tgctgtttta caagcattga gcggtagatt cggttctgaa aactggtaca aagtgatgat
    37621 ggagatcttt aactataata cagcacctgg tgctacgatt tatacgattc tgatcgttgc
    37681 cttcactttc ttctacgcct ttgttcaagt aaaccctgag aaattagcgg aaaacttgca
    37741 aaagcaagga agttatatac caagcgtacg ccctggtaag gggactgaag agtatgtctc
    37801 aagattactg atgcgtttaa gtacagttgg tgcagtcttc cttggtttag tggcattgct
    37861 tccaatcatt gctcaaatgg tttgggatct tccacaatca atcggattag gcggtacaag
    37921 cttactgatc gttatcggtg tagctttaga aactgcgaaa cagttagaag gcttaatgct
    37981 gaaacgtaaa tatgttggat tcattgagta agaaagcgtg ttgaggtttc ccctcaacac
    38041 acgttctcac acatttatta ggagggaaaa caatgaacct cattttaatg gggctgccag
    38101 gcgcaggtaa aggaacacaa gctgaaaaaa tcattgatgc ttatggtatt ccgcacatct
    38161 caacaggaga tatgttccgc gcagcgatgc aaaacgaaac agctcttggc ctggaagcaa
    38221 agtcttatat ggataaaggt gcattagttc ctgatgaagt aacaaatgga atcgtgaaag
    38281 agcgactagc tgaaccagat acagagaaag gcttcttatt agatggcttt ccacgtacgc
    38341 tagatcaagc aaaagctctg gacgcaatgc taaaagattt gaataaaaaa attgatgctg
    38401 tcatcgatat ccatgttggt gaagaaatct tggtcgaacg tctagccggc cgatttatct
    38461 gcagcaattg tggagcaact taccataaaa ttttcaaccc tacaaaagtt gaagacacat
    38521 gtgatcgttg cggtgggcat gaattctatc aaagagaaga tgataaacct gagacggtta
    38581 aaaatcgtct ggctatcaac atcaaaaata gtgaaccaat cttggcttat tataaagaac
    38641 aaggtttgct aaacactatt gatggtgctc gtgaaatcga tgctgtattt gcagatgtac
    38701 aaaaaatcat tgaaaaatag cacgattgcc cgtagccgac ttgcattatg cagttactct
    38761 tgtcaagttc aataaattat gatagaatat gtcagtccga cttctagaga atcctctaga
    38821 tgatccattt tgagaatttt gaggtgggaa ctgcagtttc cgccatttta tcgtgtgaaa
    38881 atgcgaaaca agtacaagga ggtactgtgc gtggcaaaag aagatatgat tgaagtcgaa
    38941 ggtacagtcg tcgaaacttt gccgaatgca atgtttaaag ttgaactaga aaacggacac
    39001 caagttcttg ctactgtttc aggtaaaatt cgtatgcatt acatccgtat cttacctggt
    39061 gacaaagtaa cggttgaatt gtctccatac gatcttaacc gtggccgtat tacttatcgc
    39121 tttaaataat tgtactccgt aaaactacag gaggtattat catgaaagta agaccatcag
    39181 taaaaccaat gtgcgaacat tgtaaagtaa ttcgtcgtaa aggacgcgtt atggtgattt
    39241 gcccagcaaa tccaaaacat aaacaacgtc aaggataatt ggaggtgcta taaatatggc
    39301 tcgtattgca ggagtagata ttcctcgtga taaacgtgta gtagtttcac ttacttatat
    39361 ctatggtatt ggtaacacta ctgctaaaaa gatcttagct gatgctggtg tatctgaaga
    39421 tgttcgcgtt cgtgaattaa cgaatgaaca aacagatgct atccgtgcag aagtagataa
    39481 attaaaagtt gaaggggatc ttcgtcgtga agtgaacttg aacatcaaac gtttgatgga
    39541 aatcggttct taccgtggaa tccgtcatcg ccgtggattg cctgttcgtg gacaaaacac
    39601 gaaaaacaat gcacgtactc gtaaaggccc agctcgcgct atcgctggca agaaaaaata
    39661 attcttaagt gaaggaggtt aaaacttcat ggcagcaaaa aaagtgaatc gtaaacgccg
    39721 tgtgaagaag aatatagaag caggtattgc acatatccat tctacattca ataacactat
    39781 catcatgatc actgatactc acggaaatgc attagcgtgg tcatcagctg gttcattagg
    39841 tttcaaagga agcaaaaaat caactccttt tgccgctcaa atggcagcag aaactgctac
    39901 aaaagcagca atggaacacg gattaaagac tgttgaagta acagttaaag gacctggttc
    39961 tggacgtgaa gcagcaattc gttcattaca agctacaggt ttagaagtga ctgcaattcg
    40021 tgacgtgact ccagttcctc ataatggtgc ccgccctcca aaacgccgtc gtgtttaatg
    40081 agtgccgctc attttgagtt ttatcagtaa cgtcattgaa caagacactt ttcgttttga
    40141 aaggggtaaa gataagaatg attgagttcg aaaaaccaag aatcgcaaaa attgatgaag
    40201 aaaaagatta tggcaagttc atcgttgaac ctcttgaaag agggtatggg actactttag
    40261 ggaattccct acgtcgtatt ttattatctt ctttgccagg tgctgccatc actaatattc
    40321 aaatcgatgg tgtactgcat gaattctcta ccatcaaagg tgttagagaa gacgtcactc
    40381 aaatcatctt gaacattaaa ggcttagcgc tgaaaatgta tggccaagaa gaaaaaaccc
    40441 ttgaaatcga tattaccgga cctgctacag taactgctgg cgatattatc gttgatagtg
    40501 atgtagaaat tcttaacaaa gatatgtaca tctgtagtgt ctctgaagga gctacgttcc
    40561 atgctcgctt aacagtgaaa cctggacgtg gttatgttca agcagatgaa aataaaaaag
    40621 aagatatgcc aattggcgtt cttccagtcg attcaatcta cacacctgtt cgtcgtgtga
    40681 actatcaagt tgagaacaca cgtgttggac accgtgaaga ttttgataaa ttgacgatgg
    40741 agatttggac agatggttca attgaaccgt tggaagcaat gagtttgtct gcgaaaatca
    40801 tgactgaaca tttggatatt tttgtgaact taactgatga agcaaaaaat gctgaaatca
    40861 tggtcgaaaa agaagaaaca caaaaagaaa aaatgttgga aatgacgatc gaagagttag
    40921 acttgtctgt tcgctcttac aattgtttga aacgtgcagg aatcaatact gtacaagaac
    40981 ttacaaataa atctgagcct gaaatgatca aagtccgtaa cttaggacgt aaatcattag
    41041 aagaagttaa agcaaaattg cacgacttag gtttaggttt acgtaaagaa gattaaaacc
    41101 ttttacgaag gagggaaacc aacgtgagtt atcgtaaact aggacgcaca tctagccaac
    41161 gtaaagcgat gttgcgtgat ttaacaactg atttattaat caacgaacgt attgtgacta
    41221 ctgaagctcg tgcgaaagaa gttcgctcaa ctgcagaaaa aatgatcact ttaggtaaac
    41281 gcggagattt gcatgcacgt cgtcaagctg cggctttcgt acgtaatgaa gttgccagcg
    41341 tgcgtgaaga aaatgaggac atcgtcattg aatcagcttt acaaaaactg ttcaacgata
    41401 ttgcacctcg ttacgcagaa cgtcaaggcg gatacacacg cattttgaag acagaaccaa
    41461 gacgcggtga tggtgcacca atggttgtct tagaacttgt ctaaaatcac actaataaca
    41521 tcactttata gatgattctt ttagagtgtt atgatgttgg aagatatttt tccgagtcta
    41581 gctcagcgct gctcctcaag cgaatatcgc ttggggagta agcactcatc ataaagtgtt
    41641 tttttatacc ttcaattacc tgtcatgaca gatatgtttg aaggcataaa aaaacatttt
    41701 aagcgagaaa taattgaaaa ttttgtcttt ttttgatagt gttagtagga actttaggat
    41761 gaagaaggag taatcatgga gccaattatt gaattagaaa agataaatta taaataccag
    41821 ccagatgatc ttcgccccgc attgaaagat gtctccttta caattgataa aggtgagtgg
    41881 atcgctatta ttggacacaa tggttcagga aaatcgacac ttgccaaaac gatcaatgga
    41941 ttgcttctgc cagaatcagg gatcgtgaaa gtgggaaatc aaatattgga tgaagaaaat
    42001 atttggacga tccgacagat ggttggaatg atttttcaaa atccagacaa tcagtttgtt
    42061 ggttcgacag tagaagatga cgttgctttt ggtcttgaaa atcaagggat tcccagagaa
    42121 gaaatgctag tgcgtgtgaa agatgcactt gagaaagtta gaatggctga gtttgcttca
    42181 cgcgagcctg ctcgtttatc aggagggcaa aaacaacgtg ttgccattgc cggcgtggtt
    42241 gccttacgac ctgatatcat tattttagat gaagcaacca gtatgctaga tccagaaggt
    42301 cgtgaagaag tgatctctac gatcaagaag atcaaagaag aaagtcaatt gaccgttata
    42361 tctatcactc atgatattga tgaagcagct aatgccaatc ggatactagt gatgagacaa
    42421 ggcgaacttg tccgtgaagg tacaccaaaa gaaatttttt ctgctggtcc tgaactgatc
    42481 gacttaggtc tagatctacc atttcctgaa aaactgaaga gcgcactgaa agaacgtggg
    42541 gttgatgtgc ccagtgaata tatgacagaa gaaaggatgg tggattggtt atggacatcc
    42601 gttttgaaca agtagacttc acttatcagc cgaatacacc atttgaacaa agagccttat
    42661 ttgacatcaa tatgacgatc aaagaaaaca gttatactgc attagtaggg cataccggaa
    42721 gcgggaaatc aaccttactt cagcatttga atgcgcttgt taagccaaca agcggtacag
    42781 tccatattgg agaacgagat attcagccgg atacggataa taagaattta aagcccatcc
    42841 gaaaaaaagt tggcatcgtc tttcagtttc cagaagcaca gctattcgaa gaaacggtag
    42901 caaaagacat tgctttcggt cctaaaaact ttggtgtcag cgaagaagaa gcactagtcc
    42961 tagcaaaaga aacattagaa caagttgggc tggatgaaag ctatttggaa cgttcgccat
    43021 ttgaactttc aggagggcaa atgcgtagag tagcaatcgc tggtgtgctt gccatgaggc
    43081 cggaagtact tgtattggat gaaccaacgg caggacttga tccccagggc agaaaagaaa
    43141 tgatggagat gttttggcgt cttcataagg aacagcagat cactattgta ttagttacgc
    43201 atttaatgga tgatgtagcc aattttgcag attatgtcta tgtgttggaa aagggacgaa
    43261 tcgttaatag cggggagccg caagaggtct ttcaaaacat tgaatggcta aaagaaaaac
    43321 aactaggcgt acctactgcc accgagtttg cagaagaatt aatggctaaa gggatgaact
    43381 ttgctacctt gcctttgaca gctgaagaat tagcagacgc tatcgtccat tatgctggag
    43441 gaaatgccca tgatgaataa attgatattt ggtcgttata tgcctggaga ttccttgatc
    43501 catcgattgg atccccgagc taaattatta gccagttttt attttatcgg aatcattttt
    43561 ttagcaaata attgggaaac gtaccttttg ttggcatttt tcacattact ttctgtcttc
    43621 ttgtcaaaag tagatttgaa attttttatc cgaggcattc gcccattgat ctggctaatc
    43681 ctcttcactg tagctttgca ggtcttgttt acaagtggcg gagaaactta ttggagttgg
    43741 ggtgttttcc atatcactga atttggtgtg atgaacggcc tctttatttt ctgtcgattc
    43801 gtgttgatca tttttatgtc gactctactc acgcttacta caccgccttt ggaattatcg
    43861 gatgcgattg agtacttgct tcgtccattg aaagccgtac ggttcccagt ccacgaaatt
    43921 tcattgatgc tgtcgattgc tcttcgtttt gtgcctacat tgatggacga aacagaaaag
    43981 atcatgaacg ctcagcgcgc tcgcggtgtt gattttggtg aaggaaacct agttcagaaa
    44041 atgaaagcag tcattccttt gctgattcct ttatttgtca gcagcttcaa ccgtgcggaa
    44101 gatcttgcga ctgctatgga agcaagaggc tacaaaggtg gggaaggaag aacgaaatat
    44161 cgtgtattgc attggcataa tagagacacg atggttatca ttgcttttgt attattaact
    44221 gtgctactga tctttttaag aggataaacg ttcagtgggc cgctatctat tcttttcttg
    44281 ctgatctgtt tgcaagaaag gatgcaagca gtgtatgaca agaaatatta gggtctgtgg
    44341 cagactaagg tcacaggcct ctttctttta tctgaaaaga aagaacgaaa aaattttata
    44401 gaaaaatcat tattccagat aaccttttag gaacaaagga gagaaaaaca tggtacgtta
    44461 caaagcaatc attgcgtacg acggaacaaa ttttaatgga ttccaaaaac agccaaatgg
    44521 gcgtactgtt caagaagaag tagaaaaaac attacaaaaa atggcaaatg gaaaagaaat
    44581 cacggtcttt ggttcgggaa ggacagatgc cggagtacat gcgatcgggc aagtaatcca
    44641 ttttgattat ccagaggagc gtccgttaga acgaatgcgt ttcgcgctag atacccagtc
    44701 accagaggat attgctgtaa gacaagtaga aattgtttca gaagacttcc acgcaagata
    44761 tttggtcaaa gaaaagacgt atcaatttcg agtggatata gggaaaccac gcagtccttt
    44821 tcgacgccat tatgcaagtt attttcctta cccacttgat ttggaaaaaa ttcagcgggc
    44881 gcttcctgat ttattaggga ctcacgattt cacctctttt tgtgcatcag gaagctcgat
    44941 agaggataaa atacgtacga tctacgaggc taagatggag gtaaatgaaa caaaagacga
    45001 attgttgttt acatttaggg gaaatggctt cttgtataag atgatacgga tcttagtagg
    45061 aacgcttttg aaaattggca atggccgatt gcctgaagat agtatcccag atattattgc
    45121 gaaaaaagat cgtaatgcag caggacctac agcacaccct gaaggacttt atttgtatga
    45181 agtgaaatat gaataagagg ttatgataga agtggtcagc ttcaagaaat aagaaggtat
    45241 ttacgaaaat tacttctcaa aattttgcaa aatttcggct tattttcgaa gaagctactt
    45301 ctggaacacc gtttaagaaa gaggtcgtga gcctgtcatt taactctgag tcacaaagag
    45361 caatttcaaa aaacagcttc tcatctttga tgaatatcgt gacttgtgga cgaggcgttg
    45421 ataagtgaat catctttttg ttacctattt tttctgtaac caaaaaacga tggaacgttg
    45481 ttttgattgc ccttggtgcg gacagtctga acaggttcca tcgtttcttt attttctacg
    45541 tcttcttctg tgtgtgtttc gtggttgttc ttctattgct ggatcaatcg tgccataaaa
    45601 ggggcgattg cggtcaaggt accagacata aagaataaac aatgcaatac ataggacaaa
    45661 aaggattgca cctatcgtaa atccttcagg caagtaactg aaagtgacag tatgtttacc
    45721 tgctggaact tttatcatca agaatgcgtc tttgaaattg ctgaccttga ctggtttccc
    45781 atcaatttta gctttccatc ctttgtcata aggaatagtt gtcaccaaca tggaatcttc
    45841 agcggtattt acagtcccag aagcttttct gccagtggct tccatttcga cacctttttc
    45901 tttgatctga ttcattgcgg cttgataggc tacaacatct agaccaacga ctttaggctc
    45961 catgaaactt atttctttcg tcccataaaa acttgcagta aatgtcactg ttgtctcttg
    46021 aggataatag ccaagatcat agtattgacc agaaatgttg atttgcgttt ttcgcgatgt
    46081 accattgaca gtgatcgtcg cactagaact ttctaattgc gcaaaattgg ttgggaacaa
    46141 acttaagtaa gcttgtgtat ttgcaggcac atggacttcc caagtgagct cttttggctg
    46201 gttatctgct gtttctgagt aagtgacgcc agtcgttgtc tttgtgatcg tcgtattcaa
    46261 ctgattcttc aaagtaggtg tatagaatgt aaaatactgt tcatttagac cagacaatgt
    46321 attgatcaag tttgtctgac tggtcaaatt atctgtcact ggctgctcaa catcattaac
    46381 ggacaacggt gcagtaaatg ctagtggcaa tgcattgaca ttctgataca gtgtatagtc
    46441 gccgctttga ccagcttggc ggaagccata tttactgaac gtgctttcgt ttttagaaat
    46501 gttatatttg attcctgtaa acccatccat cagcaaagta ttattgttat aacggatatt
    46561 caaatttgtc cctcttgaac ggaaacctaa tttatccagg taagaggaag aattacgatt
    46621 tcttatagaa gaaaacaaac tgataccact gtaaccatag ttgatcgaat cgtttgcaga
    46681 tactggatcc agattttcca agcggtaaaa cgagtcggaa tctttttttg tttcatttac
    46741 aagagctttc aagtcaggat aaggatcagt atataaactg cgggaagcat aattccaatc
    46801 atcaagaatg ccatggatca tactgttcgt attgaccagt gcttcagcag acatgatcaa
    46861 taataacaat atactcagat gattgaatga aagtttttta tgctgataaa atcccacagc
    46921 aaaagcataa atccctaaga aaaccactgt caatacaaag gaagtcatcg tcacataggt
    46981 gtagcttcct tcaggtttga tgccccatgc caccgcaaag agagcagcta cgaccaaaat
    47041 gattcctaac aacagcccac catcgtcttg ttctatcttt tcccatcctt ttcctgcaag
    47101 caggagcact aagaaagaga acagataggc ataacggaat aaaaacatat tcggtgcatg
    47161 cataccatgc cagaacaaat tcaatggaac aagataaaaa cttgcgataa gtatggcgaa
    47221 aagactgccg tacaaaaatt tatttttcaa cggtactttc cgactggcaa aataaaacag
    47281 acagaggact aatggtagca gacctgcgta aacgaacgga atcgatccgt attttgtcgt
    47341 gtcataaaca ccaatcatat ttttcatgat tagatccaga taagaagtcg cttctgtttt
    47401 caacgtagtg atctgactca gtgcctcgcc atttgaccga agatccagca ctgctggcaa
    47461 tacaatgatc attgaagcgc cgcctgccag taaagaagtg ataccataag gaacaatcgt
    47521 ttttttatat tctgaccagt ttgtcaatag acgggcgata aaatataaga aagaaaatac
    47581 gccaatcata aaacccatat aaaaactgga taaaaacaag agtaaataac tgataaataa
    47641 aagtttcggc cgtttaagat ccataacacg atgaatcccc aaaatcacta atggaagata
    47701 aacaaatgca tccagccaca tgatgatctc cgaatgtgca gtgataaatg agatcaatgc
    47761 gtaactgacg ctaagtgcaa catgccccca gcgaggaatc ttgaacgtac gtgcaagaaa
    47821 ccaaaaagcc aatccggcac agccgacctt aagcaaagtc agaacataca gtgcatctgg
    47881 catcatctgg tttggaaaaa agaacaccag tggcgtgaag atcccgccta gatagtaaga
    47941 aattaaagat aaataattca atcctagaga agcattccat gtataaaaaa tactttgctt
    48001 tccaagaaga acatttcgga agctggcatg aaaattagaa aactgtgaaa aggcatcact
    48061 tgccaaaata cttctgctgc ttccaggata gattccgata cttagataaa caagtgctaa
    48121 aatcaaaaac ggaatcagga aactagctgc tgcgtagcgt ccgttttctc taaagaatga
    48181 ctttaaattt tttttcatat ttattttcca ccaaatttct atgaagttgt attcagttta
    48241 tcatatcctt gtttttctct gcttaaaaag agtctagttt tcacaaaaaa ataaaattta
    48301 gaataaataa cttcataatt tgttcgtata acaaaaataa acgcaaaaaa agggtaggaa
    48361 tcaaaagtcc cacccttaaa aatttttatt aaataaaaaa ccgttcacta tctgaacggt
    48421 ccaagtatca ttcaattaaa cgcgttcaac ttttcctgat ttcaaagcac gagttgacac
    48481 ccaaactttt ttaggttttc cgtcgaccat aacgcgaact ttttgtaagt taggtttaac
    48541 agtacgtttt gttgagttca tagcgtgtga acggttgttt ccactttttg tcttgcgacc
    48601 tgtaaagtaa caaacttttg ccatggtaat ttcctccttt gccttgattt ggagcaatag
    48661 aataattcag ctcataccag actaacatat catatagaat gggaatttgc aagtacattt
    48721 tcatagatac aattgacaat tgagaagaat aattgtaaat taaacaaggt tatacctgaa
    48781 attttgcaag gtttttgagt actttgggta actgcctttt atttcaagtt gcgctatgat
    48841 aaaatatgat agaagaaaaa tgggagtttt ttacccgtta aggaggctca ttcatggctg
    48901 taaaaattca aacgtcagct ggaaccattg aaattaccaa tgatgtaatt gcaacagttg
    48961 ttggtggcgc cgcgacagat gtgttcggtg ttgtaggaat ggccagcaaa aatcaaatta
    49021 aagataatat tactgagatt ttgcgtaaag aaaattactc aagaggagta gttgtccgcc
    49081 aagaagataa tggtattgct gtagatgttt atacgattat cagctatggt acgaaaatct
    49141 ctgaagtttc ccgcaacgtc caagaaaaag tgaaatacaa tctagaggca ttattaggcg
    49201 taactgccaa ttcagttaat gtctttgtac aaggtgttcg tgtactgcca gactaggcag
    49261 gagactggct ggtaactcag cagtcgatct gaaggaggat tatctaggtg agtgtaacag
    49321 aaataagtgc aagccagttc caggaaatgg tccaggcagg tgcgaatcgt ctgcaagtaa
    49381 atgcagaata cgtcaattca ttaaatgtat tccctgtgcc agatggcgat acgggaacaa
    49441 atatgaatct atctatgact agtggagcta aggcagtagt agattcaacc tcagaaaaag
    49501 taggagaatt agcagcaatg ctttcaaaag gattgctgat gggtgcgcgc ggaaattccg
    49561 gcgttatttt gtctcaattg tttcgtggat tttctaagca aattccagat gttgtctcat
    49621 taaatgcttc tgatcttgct gcagccttca ctcatggagt tgaaactgcg tataaagcag
    49681 taatgaaacc agtagaagga acgattttga cagtagctag agaagcagcg aaagctggag
    49741 aaaagaaagc aaaaagtact gatgatgtcg ttgaagtaat gacagctgtt gtaaaaggag
    49801 caaagcgtgc tttagctaaa actccagact tgcttccagt cctaaaagaa gtcggtgtcg
    49861 tagacagtgg agggcaagga ctgctgtttg tttatgaggg ctttttaagt gcattgaatg
    49921 gagaattcca agcggatgaa acatatgaac cttctccagc agaaatggat gatatggtca
    49981 atgcagaaca tcatcgcagt gtccaaggtc aaatggcgac agaagatatc cattttggtt
    50041 actgtacaga aatcatggta aaaatcggag aaggacctac agtcgatagt acattcgatt
    50101 acgaagaatt ccgtaactat ttagatggta taggagattc tttacttgtg gtcaatgatg
    50161 acgagatcat caaagtccac gtgcatacag aaaacccagg agaagtcttg aattatggac
    50221 aaaaattcgg ttcattagtc aaaatcaaag tcgacaacat gcgcttgcag catgagacga
    50281 ttttggaaca cgatgaagaa gtgaacgagt ttgcgaaaaa agaaatacca cgtatcccgt
    50341 atggtatcgt agcgatcgct gctggtaaag gcgtccaaga gctgtttgaa agtttaggcg
    50401 ctaattatgt tatcagtggt ggacaaacaa tgaatccaag tacggaagac attgttaaag
    50461 cgattcaaga agtaaacgca gataaagtga ttgtcttgcc aaacaataag aacatcttca
    50521 tggcagctga tcaagcagca gaagtagcag atatcccagt agcagttgtt ccttcaagaa
    50581 cgatttccca aggattgaca gctatgcttt cattcaatga tcaagcttct ttagaagaaa
    50641 acaaagaaac gatgacagaa atgttgtcta gcgttgtgag cggtcaagtc actcacgcga
    50701 tcagagatac ctcaattgac ggtgtagaga tcaaagaagg agactttttg ggcatgatcg
    50761 atggaaaaat cctgatctct aacccagata tcacagcaac agcgttaagt acactagaac
    50821 aaatgatcaa tgaggacaca gagatcgtaa cgatcttggc aggtgatgaa ggaacagttg
    50881 aacaagcaaa tgaatttgct gagaaactaa cagaaaaaca tcctgatttg gaaatcgaga
    50941 tccaccaagg agatcagcct gtctatcctt atctattttc tgctgaataa tttctgtgtc
    51001 agaatatctt tctaacacag tattcattca taaaaggcct tcggttagtg atcaggtatg
    51061 atcgctgacc aagtcctttt ttt
//
LOCUS       DACDXO010000015        46031 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E23605_2017
            SAMN12639989-rid9881463.denovo.015, whole genome shotgun sequence.
ACCESSION   DACDXO010000015 DACDXO010000000
VERSION     DACDXO010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639989
            Sequence Read Archive: SRR10023389
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 46031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741914.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:42:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,972
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 33 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 17 of 73
            Pseudo Genes (multiple problems)  :: 10 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46031
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639989-rid9881463.denovo.015"
                     /isolate="E23605_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(63..914)
                     /locus_tag="IWM35_001019"
     CDS             complement(63..914)
                     /locus_tag="IWM35_001019"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HAP9918885.1"
                     /translation="MELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADHLFFE
                     EITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAGLGGG
                     STDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQVLPS
                     MPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMGNSLE
                     DITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGFCEEV
                     YLVRTLQ"
     gene            complement(1068..3641)
                     /gene="mprF"
                     /locus_tag="IWM35_001020"
     CDS             complement(1068..3641)
                     /gene="mprF"
                     /locus_tag="IWM35_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HAP9918886.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLS
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            3782..4588
                     /gene="proC"
                     /locus_tag="IWM35_001021"
     CDS             3782..4588
                     /gene="proC"
                     /locus_tag="IWM35_001021"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HAP9918887.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLIDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            complement(4667..6223)
                     /gene="rny"
                     /locus_tag="IWM35_001022"
     CDS             complement(4667..6223)
                     /gene="rny"
                     /locus_tag="IWM35_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HAP9918888.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
     gene            complement(6634..7683)
                     /gene="recA"
                     /locus_tag="IWM35_001023"
     CDS             complement(6634..7683)
                     /gene="recA"
                     /locus_tag="IWM35_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HAP9918889.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFGNPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            complement(7792..9033)
                     /locus_tag="IWM35_001024"
     CDS             complement(7792..9033)
                     /locus_tag="IWM35_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HAP9918890.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            complement(9273..9851)
                     /gene="pgsA"
                     /locus_tag="IWM35_001025"
     CDS             complement(9273..9851)
                     /gene="pgsA"
                     /locus_tag="IWM35_001025"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HAP9918891.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            complement(10060..10944)
                     /locus_tag="IWM35_001026"
     CDS             complement(10060..10944)
                     /locus_tag="IWM35_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9918892.1"
                     /translation="MAFETIGEKLRQARLNKKISLDELQQITKIQKRYLEAIDNDDFD
                     QLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQPETVSTSRKAMHQ
                     EESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDTASSLKVEGSVEES
                     SSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITDATKPVTLEFTAVN
                     RVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNATIKANGESVPVNP
                     GENNQSPKNVNLTLQYAE"
     gene            complement(11722..13008)
                     /locus_tag="IWM35_001027"
     CDS             complement(11722..13008)
                     /locus_tag="IWM35_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HAP9918893.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(13001..14269)
                     /locus_tag="IWM35_001028"
     CDS             complement(13001..14269)
                     /locus_tag="IWM35_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HAP9918894.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYSDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            14521..14982
                     /gene="mscL"
                     /locus_tag="IWM35_001029"
     CDS             14521..14982
                     /gene="mscL"
                     /locus_tag="IWM35_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HAP9918895.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(15042..15488)
                     /locus_tag="IWM35_001030"
     CDS             complement(15042..15488)
                     /locus_tag="IWM35_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9918896.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGE
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
     gene            complement(15581..15826)
                     /locus_tag="IWM35_001031"
     CDS             complement(15581..15826)
                     /locus_tag="IWM35_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9918897.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
     gene            complement(15942..16520)
                     /locus_tag="IWM35_001032"
     CDS             complement(15942..16520)
                     /locus_tag="IWM35_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAP9918898.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSVISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
     gene            complement(16597..17127)
                     /locus_tag="IWM35_001033"
     CDS             complement(16597..17127)
                     /locus_tag="IWM35_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HAP9918899.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(17131..17991)
                     /gene="rlmB"
                     /locus_tag="IWM35_001034"
     CDS             complement(17131..17991)
                     /gene="rlmB"
                     /locus_tag="IWM35_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HAP9918900.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(17984..18379)
                     /locus_tag="IWM35_001035"
     CDS             complement(17984..18379)
                     /locus_tag="IWM35_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HAP9918901.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(18376..19788)
                     /locus_tag="IWM35_001036"
     CDS             complement(18376..19788)
                     /locus_tag="IWM35_001036"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HAP9918902.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(19785..20321)
                     /gene="cysE"
                     /locus_tag="IWM35_001037"
     CDS             complement(19785..20321)
                     /gene="cysE"
                     /locus_tag="IWM35_001037"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HAP9918903.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     gene            complement(20643..22103)
                     /locus_tag="IWM35_001038"
     CDS             complement(20643..22103)
                     /locus_tag="IWM35_001038"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HAP9918904.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(22273..23430)
                     /locus_tag="IWM35_001039"
     CDS             complement(22273..23430)
                     /locus_tag="IWM35_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HAP9918905.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(23464..24834)
                     /gene="radA"
                     /locus_tag="IWM35_001040"
     CDS             complement(23464..24834)
                     /gene="radA"
                     /locus_tag="IWM35_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HAP9918906.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKTAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(24897..25424)
                     /locus_tag="IWM35_001041"
     CDS             complement(24897..25424)
                     /locus_tag="IWM35_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HAP9918907.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
     gene            25654..26460
                     /gene="proB"
                     /locus_tag="IWM35_001042"
     CDS             25654..26460
                     /gene="proB"
                     /locus_tag="IWM35_001042"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HAP9918908.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            26457..27704
                     /locus_tag="IWM35_001043"
     CDS             26457..27704
                     /locus_tag="IWM35_001043"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HAP9918909.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVIGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            27780..28124
                     /locus_tag="IWM35_001044"
     CDS             27780..28124
                     /locus_tag="IWM35_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9918910.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(28241..28567)
                     /locus_tag="IWM35_001045"
     CDS             complement(28241..28567)
                     /locus_tag="IWM35_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9918911.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(29182..30384)
                     /locus_tag="IWM35_001046"
     CDS             complement(29182..30384)
                     /locus_tag="IWM35_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP9918912.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
     gene            30501..31016
                     /locus_tag="IWM35_001047"
     CDS             30501..31016
                     /locus_tag="IWM35_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAP9918913.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(31215..31661)
                     /locus_tag="IWM35_001048"
     CDS             complement(31215..31661)
                     /locus_tag="IWM35_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HAP9918914.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(31708..33186)
                     /gene="msr"
                     /locus_tag="IWM35_001049"
     CDS             complement(31708..33186)
                     /gene="msr"
                     /locus_tag="IWM35_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Msr family ABC-F type ribosomal protection
                     protein"
                     /protein_id="HAP9918915.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(33540..34169)
                     /locus_tag="IWM35_001050"
     CDS             complement(33540..34169)
                     /locus_tag="IWM35_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAP9918916.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            complement(34153..34617)
                     /locus_tag="IWM35_001051"
     CDS             complement(34153..34617)
                     /locus_tag="IWM35_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP9918917.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(35104..36372)
                     /locus_tag="IWM35_001052"
     CDS             complement(35104..36372)
                     /locus_tag="IWM35_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosaminidase"
                     /protein_id="HAP9918918.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(36603..37340)
                     /locus_tag="IWM35_001053"
     CDS             complement(36603..37340)
                     /locus_tag="IWM35_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP9918919.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(37407..37976)
                     /locus_tag="IWM35_001054"
     CDS             complement(37407..37976)
                     /locus_tag="IWM35_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HAP9918920.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            38104..38976
                     /locus_tag="IWM35_001055"
     CDS             38104..38976
                     /locus_tag="IWM35_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP9918921.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKVFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            39318..40190
                     /locus_tag="IWM35_001056"
     CDS             39318..40190
                     /locus_tag="IWM35_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP9918922.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            40187..42145
                     /locus_tag="IWM35_001057"
     CDS             40187..42145
                     /locus_tag="IWM35_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9918923.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAATRPAQTNTVVKTETFAGGTIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVKEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(42477..43328)
                     /locus_tag="IWM35_001058"
     CDS             complement(42477..43328)
                     /locus_tag="IWM35_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP9918924.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKKLEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            43451..43918
                     /locus_tag="IWM35_001059"
     CDS             43451..43918
                     /locus_tag="IWM35_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HAP9918925.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(44181..44663)
                     /locus_tag="IWM35_001060"
     CDS             complement(44181..44663)
                     /locus_tag="IWM35_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP9918926.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVIA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(44763..45626)
                     /locus_tag="IWM35_001061"
     CDS             complement(44763..45626)
                     /locus_tag="IWM35_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP9918927.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGARLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYELVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
BASE COUNT        13128 a         9840 c         8239 g        14824 t
ORIGIN      
        1 aaactgaaga ataccatttt aggattcctc agtttttcat cacctatttt acttcttata
       61 ttttattgca gggttcgtac taaataaact tcctcacaaa aaccttttaa cccattatat
      121 actctctgcg ctcttgaata atgttgacac aaggcaaaaa cggttggtcc gcttccactc
      181 atcaatgcag catctgctcc gtacttgatc atacgctctt tgatttgatg gatgactgga
      241 tgccgctttc ccgttatatc ttctaaactg tttcccattt ttttgatcat ctgctgataa
      301 tcttgctctc ggatggcttc agcaagtccg tggatgtctt gatgttggat ggcttgcgta
      361 tctacttctc taaacactgt acgtgtagag acactcattc ttggtttgac caacacaacc
      421 cagcactgtg gcatagaagg taatacttgg atttcttccc ctttgccacc gacaaatgcc
      481 gtacttccgt agacacagta aggaacatct gtaccgactt ccaaaccgat ttctgccagt
      541 tcttctaacg aacagcccag attccataat tggttgatcc ctcttagagc agctgcacag
      601 tctgtacttc ctccgcctaa tccagctgct actggaatat tcttcttaac aaagatcttg
      661 acaccggttt tgatattgta tttatttttt agcaataaag cagcttgata aacattgttc
      721 cgttcatcta ccggtaaaaa tgctttattt gtttctacaa tgattttgtc ttctgttatt
      781 tcttcaaaaa atagatgatc tgccaagtct acgctcgaca tcaccatttc caactcatga
      841 tacccatctt gccgtctata taatacatcc agtcctaaat ttatcttggc tggcgctttt
      901 tcaatcaatt ccatcctctc acccattcga aaagtctaag tcgcaagtat tctatcatag
      961 ggaaaaataa aagtatagct tatgtctgcc tagtggttga ttttgtcatc gtttcaacag
     1021 ctttcacaga attttctctt cataatccaa acaagttcta gtgattctta taattctgtg
     1081 agcacttctt ctgccaaact cggaacattt cctttttctt ttcctatcag actgagtagc
     1141 tgcatggtag caaatccgaa attacttcct ttcctataag caaaataacg tccttcccaa
     1201 tgatcgacat attttaattt cttttcataa gcagactgga aggaatagaa aaaattacca
     1261 taattatata cgatatgcat gactttttct tgaaagaaag aaaaatctgt ttctccaaca
     1321 ttagctaatg gagctaagcc tagattggct tccgagtagc ccattttctc gtatacaata
     1381 aacagattgg ctaacagata gtcagctaat tcctcagatt ctttaggtaa gacccgcaac
     1441 aaatcatatc ccgcctgctt cttcgtcatc ggctgagcgg tgatgaagcc aacgactttt
     1501 tgatctttac ggacaatacc gacaccgcta gataatacat atgatttatc aaatcgtccc
     1561 acagagaagt atttttctcg tgcaccttga agccattttt gtgaaacttc atttacagct
     1621 tgatgtaatt cttccggcag ttgatcgtac caagtaaaat ggtatccttc tttcatcaaa
     1681 cgttgatatt caagttgctg actcagccga gacaagtctt ctgttgctag atcaacaatc
     1741 cctgcttcac ctactttcgt gaagtgaaaa cccaattcat gaagcatcac tacgtagctt
     1801 tctgatatct tataaaacgc aagctgatag cctaaacaat ctgcttgttt cataaactga
     1861 ttcgttgctg cccgcatcat tttaggattg ccaactggat ctcctaaaac aaagcatttg
     1921 tttcctttga tctgataacc aaaaacgact tgatcttggt cgtcttcttg gtaataaaaa
     1981 taactgtaac ctgacaagcg aagctgatgg cttgtagctg tgcctccgta tgtttccaat
     2041 aaacggtcaa aacggtcttt ttcgtactca ctgccaagct tcgtagtatc ttcagctaaa
     2101 tattgataaa gcgccactaa agctagcaat gagatgccca aaccaataag ccctgagaac
     2161 cacacatcat ctgaaggaaa caaaataaaa tgatttcctg ttattcgacc gactaaaaag
     2221 cgtccgctgt gatagcctgc aattgcgtaa acgataaaca aaaagccgaa caagctagca
     2281 tcaaagatta tagctcccca agaataaacg aattttgttc gataaaattc ttttctggat
     2341 aaccacacag ctaacagaat caagacatat accaatatca actgccaaga taacgtacgt
     2401 gtgattgtat ttaaaatacc aaaagatagt aaagcaatcg taggcagaaa tgcctttttg
     2461 acttttactg aaagtgctct agcaaggccg agtaataaaa agccaaccaa tagattcact
     2521 gtctgatcta aaaaattgaa tgagaacggc aataatactt gaaatagccg actgattgca
     2581 gacagattag tgattgttga tagaagtacc atcatgatcc ccgcaaaata gacagcactt
     2641 acgagcacga tatgcgcaac cttttggaaa aacagtcgcg gaatgttatc taaaaatcga
     2701 ttgattttga ctcctgtatg tgtgatgaac aatagaagtc ccgtcataaa aggtagaaca
     2761 taataaaaca accgataata taatagccaa actactgcta catcttgccg aacagacaat
     2821 tgactcattc ctaagatcat caaaacgtca aaagtcccca ttccacctgg gaccatcgtc
     2881 agcattccta tcaatgtagc aatcatgaac ataggataga tcgaaagcat cgaaatatct
     2941 attctcatca acttgccgat gatcaaaaat accgcaaggg ctcccgtcca ttgtccaaag
     3001 gatgccagta gcagaccaag caagcctttt ggataaaact cgaagaacaa tgtctttctc
     3061 tttagataag caaaaaagaa aatgactggt gctaaaaaac taccgccgaa aagccagatc
     3121 caatagcttc gaaaaatact ttctgactga ataaaaaaga catcaataaa tgtaagaaac
     3181 gaccatacag acaaaccagt cagcataaat agtgcgactt ttgatacagt ggctaaaacc
     3241 atttttctat ttgtctgctt tccataaaaa ctagcccgca gacttgctcc taccacacca
     3301 ccaaaaccgg cgagattgtt gatcgtgttt gttacccacg cagcaagaag aaactctttt
     3361 ctttccattt ttggttttcc ttgtttttct aagactctga tcgtcaccca atcatacaac
     3421 aacatcggag tgacacccag cagtccagcc agaatcatga gcaggatcgt catgcggctt
     3481 tgttgtccca tcctttgcca aatatcttgc caactcatgc cattagcaat atttgcgact
     3541 tgatttgcca caaagactaa cacggagccg aaaaaaatca gttttaaaat cagactatgt
     3601 tcctttatcc attgcagcat caagtatcgt ttatttttca cattttccca ttcctaactt
     3661 gtatactttt gtgtcatacc ccaatgttat cttatttatt ttaatttttc cactttagac
     3721 ctgttctttg cttgcgatga tagaaaaaga aagatatcct aatgctaaag gagaggataa
     3781 catgaaagta ggatttatcg gcacagggaa tatggcacaa gctattattg atggtttatt
     3841 aaaaaaagga acagtctcga aagaagaggt gttcgtttcc agtggacatt ttgaaaatgc
     3901 acaaaaattt gctgctgaaa ctggcgtcca tgcatgcaaa agtaatcagg aagttgcatc
     3961 acaatcagat atcgttcttt tagccgtgaa accaaaaatc attgaaaaag tattggaaga
     4021 actaagagaa tatagccaga aagtcttatt catttctatt gcttcaggaa aatcattaat
     4081 agatttagaa acagcgcttg aagatcctca ggcctccatc gtacgtgtga tgccgaatgt
     4141 caatgtcagc gtaggagcag gcgtttctgc gatttgcaaa aatgaagctg ccacggagga
     4201 acagcttcag caagctacga tgatttttga agcaatcggc agcgtctatc ctttagcaga
     4261 atcagatttc agtacattca ttggccttgc tggcagctca cccgcattcg tttacatgct
     4321 gatcgatgcg atgggacgga ctggtgtact gaatggtttg tccaaacaga cagccactga
     4381 gattgctgca aaagcaatta tgggttcttg tcaaaaattc ctttcaagtg ataaaaatcc
     4441 atgggaactt gtagaccaag tttcttctcc tggcggtaca actgttgcag gtgtagttgc
     4501 cttagaagaa gcaggattca tcccagcaat catcaaagga atcaatacga cgatcgagaa
     4561 agatcttgag atgttgaata aaaaataatg atagcaatgt tttttcgtgc cttacaaaaa
     4621 aagaagagct gtggcttttg ccgcggctct tcttttgtat tttttattat ttagcataat
     4681 cagttgcacg agtttcccga ataacgatta ctttgatgtg tccaggataa tccagttctt
     4741 cttcgattcg tttacgaata tcacgtacta aacgaacagc gtctaaatcc gagatttctt
     4801 ctggcttgac cattacccga acttcgcgtc cagcttgaac tgcgtaactt gattctacac
     4861 cggcgaagct gttcgaaata ttttccagac ttcttaaacg acggatgtag ttttctaatg
     4921 attcacttcg cgcaccaggt cttgctgctg agagcgcatc tgctgcagct acaaggacag
     4981 agataacaga agtagcttcc acatctccat gatgagaagc gatcgcgttg attactactg
     5041 ggttttcttt gtacttggca gctaattccg caccgatctc aacgtgagat ccttcaatct
     5101 catggtcaag tgctttccca atatcatgga gcaacccggc acgttttgct aattggatat
     5161 cttcgccaag ctctgctgct aagataccag ctaattttgc aacctcgacc gaatgcttca
     5221 agacgttttg tccataacta gttctgaatc gcagacgtcc gatgatcttg attagatctg
     5281 gatgaagtgt atgagcaccg acttcaaacg ctgcctcttc cccgtattcg cgaatacgtt
     5341 catccatttc tttacgagat ttctcgacca tttcttcgat acgcgctgga tggattcgtc
     5401 catcttgtat cagtttttcc aaggtcatcc gtgcgatttc tcttcggatt ggatcgaatc
     5461 cggaaagaac aactgcttcc ggtgtatcat cgatgattaa atcgatacca gtcagcgtct
     5521 caagcgttcg aatattacga ccttctcgtc caatgatacg acctttcatt tcatcattcg
     5581 gaagggtaac gactgaaaca gtcgtttctg aaacttgatc tgctgcgcag cgttgaatcg
     5641 ctaatgataa taagtttttc gctttgcgat ctgcttcttc tttggcacgc tgttcagatt
     5701 cttttaccat tagagttaac tcatgattca gctcttcttc agtcgatttc atgatgacat
     5761 ctcgtgcgtc ctcttttgat aaaccagcaa ttttttctag ttctttttgt tgatcttcaa
     5821 tcattttttc aacgtctttt tctcgctctt caattaattg ctgttttgaa ctaagtgctt
     5881 cttctttact ttcaagtgag ttctcgcgtt tttctaacga gtcatcttta cgattcagcg
     5941 tttgttcgcg ttgaatcaaa cgattctctt gagattttaa ttctaactta ctttctctca
     6001 gctcactttc aacttcagaa cggtatttct gattttcttc cttagcttct aacaatgctt
     6061 ctttttttag agtttctgct tctttttttg cactatcgat gattccagca gctgatgatt
     6121 gtgcaccagc tatttctttc tcgtgacgtg actttgcgac cataaaccct aaaccaagac
     6181 cgacaattaa accgatgata gcgaggagaa tgttgaatac cataaaatcc acctccatac
     6241 tatctttttc atttttatca tctatacagc ttctatgata aattattcta aactcgtcaa
     6301 tatgcatctt cgcatatatg tgtacaaaaa acatgcacaa ctttattcta atgtttgtac
     6361 cccgtagtgt caactttaaa taaacaaaga aacggctttc tacttattca ttaaacgatg
     6421 gtttttaaaa taaatgacac tagtttcata taggtaaacc tttaaattga gaaacttttc
     6481 tattaaatta ttctttgtag atgaacagat atttttattt tatgtaccca ctcgtataat
     6541 taaataactc acgatttctt tattcaataa aaaaacggca ggtcaaggag agtacctact
     6601 cttcagacca gccgtttttg tttatcataa tttttattct tctactaaag gcaattcttc
     6661 ttgtcctttt tcatcttctg ggacagcgac ttcttcacca ataccataag ctgctctaac
     6721 tagagcagaa acttctgcca tcatttcagg atgattagcc atatagattt tagcgttttc
     6781 ccgtccttga ccaatccgat cttctttata agaataccaa gcaccgcttt tatcaacaat
     6841 gtctttttct actgccatgt ctagaagttc tccttcttgt gagatcccta aaccatacat
     6901 gacatctact tcagcgattt tgaaaggagg tgctactttg ttttttacga ctttgatctt
     6961 tgtacggtta ccaacaatat ctgtaccttg tttcaattgt tctgcacgac gtacttccaa
     7021 acggatcgtt gcgtagaatt tcaatgcgcg tcctccagga gtgatttctg gattcccaaa
     7081 catcacgcct actttttcac ggatttggtt gatgaaaata gcgattgttt ttgttttgtt
     7141 gatcgaacca gagagtttac gcaatgcttg agacatcaaa cgtgcttgta acccgacatg
     7201 tgagtcaccc atttcgccgt cgatttctgc tcgtggaact aaagcagcaa ctgaatcgac
     7261 tactacgata tctacagccc cacttgatac taaagcatca gcgatctcta gaccttgttc
     7321 tcctgtgtca ggctgtgaaa gaagtagttc atcgatattc acacctaatt tttgtgcata
     7381 ttgcggatct aacgcatgct cagcatcaat gaaagcggcc gttccgccat ttttttgtac
     7441 ttctgcaata gcgtgtagtg caactgttgt tttacctgaa ctttcaggtc catatacttc
     7501 gatgatacgt ccacgggggt aaccgccgac acctaaagcg acatctaacg caagagagcc
     7561 acttggaatt gtagagattt gttggtcgat tttttcgccc aatttcataa tagagccttt
     7621 accatagctc ttttcaattt ttttcagtgc agcatctaag gctgctttac gatcatctgc
     7681 caatacgata atcctcctta tatttacaaa tcagagcaac atttcattat ccttatcata
     7741 tcttttttag tgaaaaaaag caagaaaaaa ccgaacaaat attcgcattt atcattttct
     7801 taaaagctgt tttctcaaaa gatcaaatcc ttgcataacc gcattatatc tgatagcagc
     7861 acgatctctg gcaaagtgat atttttccga aaccacgccg tctttagatg aaatagcgat
     7921 ccatactgtt cctgcagatt gaccttctaa cgtgtccggt ccagccactc cggtgaaagc
     7981 tacactgtaa tctgttcttg ctatctcacg tgcttgaatg gccatctgct ccacacattc
     8041 tttgctgact gttccatatt ctgctaatag ctccggatca atctccagaa aaccagcttt
     8101 tgtttggagc gagtaggtaa cgaatccgcc tggaaatact tcagatacac cagcaatgtc
     8161 gcctaatgcg gcttggaagg cgccggctgt caaactttcc gctgctgtta cagtctgctt
     8221 gttttctttc agtagttcca ctacgacttt tgctagcgaa ttatcatcgc cgtatccata
     8281 aaaatattcc cctactcgtt cttggatttt ttcttctaat gctaaaagag cttgattccc
     8341 tgcctgaaca tcatttgttt tcactgtcag tcgtaatgtc acttcatttg gtttggcata
     8401 tggtgcgatc gttggattga tctgtgtctc aatcagatct tttagctcag tgaccagtcg
     8461 cgattcacca atcccataaa agcgcaaaac tttagaaatt agtttttctt cagaaggaaa
     8521 acgttcctct agtaaaggac gtacttgttc cacaaacatt ggtttcaact cattaggcgg
     8581 accgggaaga aggatatacg catgtgtatc tgtttgataa aaagtcccta aagcaagccc
     8641 tgtacgattt ggtagaggaa ccccgccttc gataatctgg gactgttgca gattgttttt
     8701 ggtcattttt cgatgggttg tttcaaaata tgccaataat tttttgtatc cttccgtatt
     8761 ttgtatgaga gacttaccga ggtgagccgc agttgcttct tttgtcaggt catcttctgt
     8821 cggtcctaat ccgccacata agagtatgag ttcacttctt gtttcagcta gagcaatcaa
     8881 ctcttctaaa cgctgctgat tatctcctac aactgtttga taataaactt cgatacctag
     8941 atcagctagc tgttctgaca agaaagtggc attcgtattc acgacttgtc ctagtaaaag
     9001 ttctgttccg acagctatta tttctgcttt cattacttcc cgctccctta tatagtatac
     9061 gctgtatttt tatatttttt tatttaaaaa gagggcaagg agctgtgaca aaaatcttgt
     9121 cacagctcct tgttccaaat aaacagtgtt ctcctgccaa aacctcggtc ataagtcaca
     9181 atatacataa aacatgaaaa gctgtttttt gaaattatcc cttgtgactc agagttagac
     9241 ggcaggctca caacctctcc actaaacgat acttacattg atcctttgaa cacaccgctg
     9301 tttttgatga agtaatccac acctgaatag atagtaaaga tcagacaagc ataaagcatg
     9361 atttgatcta ctggaatacc gatcaggctg aatgggatat tattgataaa cagcaagata
     9421 atagcaacca tttgtgtagc cgtcttgact tttcccggcc aagctgcagc catgacttct
     9481 ccatgttcga caagtagtaa acgcaagcct gtgacggcta attcacgaca gacaatgatc
     9541 gctactaccc aaccaggggc ctttccttgg tcaaccaaca tgataaaagc agtcataact
     9601 agcattttat ctgccaacgg gtccgcaaac ttgccaaaat tagtgacaag gcctcgcgca
     9661 cgggcaattt ttccatctaa ccagtctgtg atactagcta aagcaaaaat aactgcacct
     9721 actagctgtg tgacagccag tgtggtgtct cctacgttaa attgtcccca atctaaaggt
     9781 aaacctacaa ccaaaatgaa aatcgggatc atacaaattc taagtacagt taatttattt
     9841 ggtaaattca cactatcgac tcctttcttc cctattctac gtgactatca gcgaagtttc
     9901 caccgatatt atttactttt ccaaatgaat ttattcaaag gagtattttt attgaaattg
     9961 atctttttga attaacgaag aaggaccgcg acacagattt gtcacgatcc ttctttttta
    10021 tacctacaag taaacagatg cgtaaactaa tttatacgat cattctgcgt attgaagagt
    10081 aaggttcaca ttttttggac tctgattgtt ttcccctgga ttaactggta cggattctcc
    10141 attcgctttg atcgttgcat tgcttgctgc accaatcgtg atggtcgcat ttgttgctgt
    10201 ttccggtaac acggtactct gtgtttcatt tgctgccagt gtcccttgat aaacataggc
    10261 attgtctact agcacaccta accatacacg attgacagca gtaaattcaa gggttacagg
    10321 cttggtagcg tctgtgatat ttactgcgat ggcactgcca gaatcttctc ctgtagctat
    10381 tgccattttc ttttcttcac ttgattttgt tgttgattct actgtagaag actctttggt
    10441 ctgcgtgctt tgctctgagg aagaggactc ctcgactgat ccttctactt taagcgaaga
    10501 agctgtgtct ccgattattg gatcagcttg acgatcttgc caagtcatat agcccacgac
    10561 aacgacaatc gccaaagcaa ccaatcctaa taaaatgaca ggaagacttg tccaaaagcg
    10621 acttggatgc gattcttcct gatgcattgc ttttctagaa gtacttactg tttcaggctg
    10681 cggacgttca gggaccgatc ctgtcaattt gcttttccca tcaaaaacat cgactaaatg
    10741 gtctccatct tctcctacag cttcagcgta ttgccgaata aatgcccgca cgtaaaattt
    10801 accaggcaat tggtcaaaat catcattgtc gattgcttcc aaataacgtt tttgaatttt
    10861 tgtaatctgc tgtaattcat ctaaagatat ttttttattc aggcgtgctt gccgtaattt
    10921 ttctccgatg gtttcaaaag ccactcgttc atcttctcca gtctgtttga ttctccgaag
    10981 catctgctgt tgcaacgaaa gacgttatgt ttgggtgctg tgacaaaagt catgtcacag
    11041 ctcctttttt ccgaataaac attgttctcc tgccaactcc tcggtcacaa gtcacaatat
    11101 ccaataaata taagaagctg ttttttgaac ttgttccttg tgactcggag ttaaacggca
    11161 ggctcacgac ctctttctta aacggtgttc aatcagctgt tcctcggtat tagctcaaaa
    11221 accggcaaaa tataaggaga tatttttccg tatgtttctc taatactcag gtcaaaacgc
    11281 tgatttcaca acctcttcct taaacggtgt tcaaaagctt gcatctgcgg taataagtcc
    11341 aaccaagtta aaaacattaa gaagctgttt ttacttggct gttcttatta cttgatgcag
    11401 ggcagctttt tcacaacctc ttggtacacg gtgttccaga agtagcttct tcggcaataa
    11461 gccgaaatat cacaaaaatt tgagaagcaa ttttcgtaaa ttccttctta tttcttgaag
    11521 ctgaccactt ctgtcacaac ctctttgatt acttcgtttg attttatttt aatataagtt
    11581 gattaaaaga gagcatgatt tgttttattt tctcactaat ttttccaaca caactaagaa
    11641 taccataaaa gctgattttg gaggaggttc ctactacaag tctaaaacga aatttaagac
    11701 ttttcctcat aaaatactgg tttattttgg tctcatgtaa aactcgctga aggcctcttc
    11761 acttataaat gcttccccag ctgccagtac gtctttcatt tggatcgaat cgatgatttc
    11821 aggaagatca aataaagttc gatcgccaaa taaactttgc gtaaattgat tggcaatata
    11881 ttcgatcgaa tttaatgatt ggaaatattg tccaagcatt ttcttcttca gcaaatcgag
    11941 atttgtttct gacacttccg gatcatcagc gaatccaaga atgatttctt tgactttttc
    12001 tgctgctttc tctggttcgt ccgtatctcc gctgaagtcc gcaaagtgaa attcacgatc
    12061 caagctgaat tcaaaaccaa agctgtcatc gataattcct tgattgtaca tcgctagata
    12121 gtttcgagaa gtattgccta aaatcatttg gaacaacaga ttgattgctg ttttatagcg
    12181 taacagttct gttccttctt gcggcaatgt gtctaatcct ttaataccaa gtacaaattt
    12241 atctcgtgta atggctgctt ccaatgcact ttgtttgatg atttcttttg tgttttcagg
    12301 gaaataacgt acaatttctt gctttggagg gaaatttttt gcttcttggt tttcccgaat
    12361 aagtttcatc aatttctcag gctccatctt accaactaca aataaaacca tattgcttgg
    12421 ctgataaaac gtccgatagc acgtatacaa gtcttgagct gtgattttgt cgatactttc
    12481 aaccgtgccc gcaatatcga tatgcaatgg gtgtgtagga tacaagttat ttagtatgcc
    12541 aaaaaacatc cgccagttag ggtcgtcttc atacatttgg atttcctgac cgatgattcc
    12601 tttttccttg tttacagttt cttctgtgaa gtaaggtgct tgtacaaaat cgatcagagt
    12661 cgtcagattc ttttctacct gatccgtcgt agaaaacaga tagctggttt tcgtaaaact
    12721 tgtgaaagca ttggcggaag ctccttgttt cccaaataat tgaaagacat caccatcttc
    12781 tttttcaaac aacttatgtt ctaaaaaatg ggcaattcca tctggaactt tcaccttttc
    12841 tttttcacca tagggaataa attcgttatc gatcgaacca taattcgtac tgaacaaacc
    12901 atatgtcttg tgatagtcat ttttaggcaa caaatacacg gtcaaaccgt ttggcagtac
    12961 ttcatgataa agtgtttcat tgatttgctc atattctgtt ttattcattt gcatttcctc
    13021 ctgctaaaaa gaagattgcc tgcaattcga tttgctcagc tacttgttgt atctctttga
    13081 tcgttacgct ctccatacgt ttcaaccatt cttcatcaga taattttgtt tccggcagcc
    13141 agctgtccaa aaaagaagct tcgattgccg cttgcggaga gtctagagaa agcagatatt
    13201 ggtttctcag cattgctttt gtctgagaca gttccaactc tgttatttca ccatttcgca
    13261 aactttctaa ttgctcgtga atcagatgaa gtacttgatt tcggttcttt tcatcgatac
    13321 cggtttggac actcatgaat cctcggaacg tatccacgct actagaggca taataagcta
    13381 gactttcttt ctctctgaca ttcatgaaaa gtttagagtg aggaaaaccg ccaaataaac
    13441 cgttaaagac cattagcgca aaacgttctg gttcatcgta atacacattt gtctgataag
    13501 ctaggttcag ctttgcttga acgattggct cacgtacttg tcgttccttc acgatattca
    13561 cttgaggttg ggtataaaac agatctggat aaactgtttc ccgcgactca aatggtaatt
    13621 gcccaactgc ttcagctgct ttgtccggat caacatcgcc gacaacaaaa atgtcgattt
    13681 gatcatttgc catcatttct tgatagtagg acgctagcga actagcagtg atcttgtcca
    13741 aatcttcgac tacgcccaac cctgggatct tttgttccgg gctgttttca aaataaagtt
    13801 cttgcaaggc ataggaagca aaagtttgtt tatcttcctt caaactttcc aaatatagac
    13861 gcagattgtt cttttccaag tcaaacgtca actgatcaaa ctgatgattt ttaatatttg
    13921 gataaaataa aacctctttt aaaaaatcta ccgcttgaga aaaaaggttt ggatcattga
    13981 catattcacc attcacaaaa ctgatccctg cgtttagcca atgtaaattt cctttttttc
    14041 taactgataa tccaaagctt gcgccataaa gctctgcaag ttgcgcactt aactttgtct
    14101 gatccgaata attcaaacta tttgtttcca acatgctgct gagaagtgaa cgtttcgtga
    14161 tgacttcttg ttgcagacgc gcagtaaaac ggataaaaat ccggatcgtt ttgtattgtt
    14221 ttgttggtaa gacatgaaga ttcacgcctt ctgccaattg atacaccata tttatatgct
    14281 ccttttcaca gttgattgcc cgcttactct tttaatgggt caacctgcct tttttactta
    14341 ttcctctgct tgaaatttat aagttcaggc aattcgccgt tttttctaaa tgatgttaca
    14401 ctttatagta tagcatagat gttttttatc aaaagaaaca gacggctaaa agcgtatatt
    14461 gtttttttat tttttatttt tcgatatact aaagacaaat aaaagaaggg agggtcattc
    14521 atgatcaaag aattcaaaga gttcatcatg cgtggagatg tcttggatct tgctgtcggc
    14581 gttgttattg gtagtgcgtt tacaagcatc gtaaaccaag tggtgcaagg cttgatcaca
    14641 ccactaatag gttggtttgt cgcaatgatc acaggtactt ctgatttaga aggagcactt
    14701 tcaattcttg actggtcgcc gacaaaaggc gtcacatttg cgtttggtaa cgttattagt
    14761 gctatcatta catttctgat cactggtttt gtcttattct tagttgtcaa agcggcaaac
    14821 cgtgcgaaga atttacgctc caaagaagag caagtggaag tggaagaaac tcctactgca
    14881 gaagattatc ttagtgacat acgcgatctt cttgccaaac aagtggcaga agacaataca
    14941 catccagtag agcaaacaaa tctttccgat acttctaaat aataaagaat ctaaaaaaaa
    15001 agaaagccga ttttcagtgt cggctttctt tttttagatt cttaacttgc tacttgttcc
    15061 tttttcatgt cgatatgtgg aatatcatct tccaaataca catctgagat tgccttgaat
    15121 ccaaacgact cataaaaagc ttgtaggtat tcttgtgcgc cgatcacgat tggtttttct
    15181 ggaaaatttt tctggatcat gttaatcgtt tcttgaacaa tctttcttcc taaaccatct
    15241 ttacggttgt cctgatgaac aaggacacgt ccaaaatgga tactctccgc ttcttcatat
    15301 actcgcgtgt aggcaaggat cttcccatca gtatcttgaa aaaacgtatg ccaagcttct
    15361 ccatcaatgt catcaacttc ttgataagga cactgttgtt ccacaacaaa aactgcgatt
    15421 cgttctttga cgatcgcgta aaactctgct gttgacaatt cttcaaattt ctttacctga
    15481 tattccacta agcaaacgcc ccctgctttt ttattcttag cataccgatt cgcacacaaa
    15541 aaagcaaacg aaccttacga atctcaggct cgtttgcttt ttaaactgct tcagttaaaa
    15601 attcgatctc aactgtccga gtaagtacat ctgaatagct gtaagatact cgttcaaaag
    15661 aattttcttc aggatccaaa tcaacaacga atactgaagg ataggtttct gttaaaacgc
    15721 cgcggcgttc tgtttgacgc tttcttcctg tctgagcaac caacatgatt gggctgccga
    15781 tacgaccttc taaatctttc ttgattgaag ctaaagttgt tggcatacag ttcacctcta
    15841 ctatcaacta taccacatat ttttaaaatt ttcaagttta ccataaaaaa aaaaagaaat
    15901 gcaataaaaa taaataagaa gctcattaag atttttctaa attattcctc attataaaat
    15961 ttcaagtgtt tttttatttt gagttttact ctcccgtaag cgctcattac ttgcttgtga
    16021 tccattgcta aacattcagc catgtctaca aaatcaacgc cttgcatata ataatgaaaa
    16081 attttagctt ctatttttga aaactggatt tcgcaatttt cgattgcttc ttttaataaa
    16141 agatgctgaa ttgctaaagg ttcttgcaat gtgatctctg ctaaaaagtc tggtccttct
    16201 gtctctattt tttggtcgat tgagacttca tctttttgat cacgacgctt cagcgcgttc
    16261 tgcttcctca acagagaata gatttgattg cttataacgc ttttataata acttccaaag
    16321 ctaacacctt tgcttttttc gtaagttctc aatgcctgat ataaagcgat ccttgcttct
    16381 tgttcccaat cctcagaaac ataatttctt aaataatagg tgtttttcaa acgtagtaaa
    16441 agtggaaaat atttttgata cagggcagca aatgcttctt cgtcaccatt acgtgcagcg
    16501 cgcaataact cctcagacat aaaataattc ctccataaat tcgtttatgg aggaatctta
    16561 tcatcaatga ttcatcgaac tgattagaac gatatgttat ttattttttc tcattaaatc
    16621 gtccaatttt tgggacaatt tttttaactg ctcttcattc catggagaat tacgtcggaa
    16681 atcaataaaa tgcatatctt tattttcttg ggctattgtt ttttccgttt tttttatcgt
    16741 cttatataat tcataagcag aagttcgtaa tgctccttgg gaaaagatca tccactgttc
    16801 tgctaaatcg cttgtcgcca ctgtcacttg tgtcaaacgg tcattcagct gaccagcgat
    16861 tctctcgata taactgtcag ccgtttcgtc ttctttcgta aaaacaacat ccaactggta
    16921 tttcttataa ttttgttgga tgcccggaac tagttgggca tcgaagacaa cgatgacttc
    16981 cattccttca tatttcgcat aatttgataa acgatgtaaa agagcttctc ttgcatccgc
    17041 cattttgtcc tgctttttaa gctggacaag ttccggccac gaaccaatca tgttgtagcc
    17101 gtctacaatc aacagctgtt ttttcatggt ctactccccc tgacgttggg aaaaagcctg
    17161 atacataagc aaaccagcag caacactggc atttaagctc tggacgtgcc cggtcatcgg
    17221 gatcgtcagc aattcatcca tttctttttt caatcctgga ctcattcctt tgccttcatt
    17281 accaatgact aaagcaattg atcctttggc attccatttt tggaagtttg tgccggtcat
    17341 atctgtgcca aatacccaaa atcctgtttc tttgagctgt tgcacagctt gtaccaaatt
    17401 cgtcacgcgt gcaacaggga catgttcgac tgccccagtg gaagtctttg ttacgatagg
    17461 tgtgattcca actgctcggt gttttggtat gatcacacca tcgacgccac ttgcgtctgc
    17521 tgtccgtaaa atcgacccaa aattgtgtgg atcttcgatg ctatccaaaa tcaaaaagaa
    17581 ggggtcttct tttttctctt ttgttcgttc aatcagttgg tcaagggata aatattcata
    17641 aggtgtaatc gcaagaacga ctccttgatg cacgccgtga tcgctcaaag tttctaattt
    17701 ttgtttagga acccatttga ctggcacagc ttgttcacgt gcgatttctt tcagttcttc
    17761 tactttttct cctcgtgaat cttcttgaag aaagagttta ttccctcgcc cttgttgtag
    17821 cgcttctact gcggcatggt gcccaaagac gaaattatct tctaatgctt cttcattttg
    17881 atcagaaact gactgatttc gtccagtttg ctttttattt gaatatctat tttgtttttt
    17941 gtcttcaaat ccacgtttcg attgatgttt gtttgttctt tctttaccca tttttttcac
    18001 caactttctg aatacaccag cggatcaatt cttccatccg ctcggtttgt tttgtcaaat
    18061 gcagatagcc catgagcgct tcgaaccctg ttgcgacccg atacgtcgta acatcagcat
    18121 tttttgcgct cgtatggctt ttagcatttc ttcctctgcg ataataaagt tcttcttctt
    18181 ctgtcaaaag atgttcttcc agcatttgct gaatcaaaaa ggcttgtgca ttcgcagaaa
    18241 cataattcgt tgccgcacga tgcagcttat tgggttttgt ttctccttga tcaatcaggt
    18301 ggtcacgaac atagatttcg taaatcgcat ctccaacata agccaaggct aaaccgttca
    18361 attgtgtgta atctctcatg cttctcttct ccatcgggtt ccttgcggtg tatcttctaa
    18421 taagatccct ttttctttca acaaatcacg aatttcgtcg cttcggacaa aatcacgatt
    18481 gcttcttgct tgatttcgtt cttcgatcaa ttgttcaatc tcttcatcta ataactcttc
    18541 tgctgtaaat aaaataccga agatcgaaag ccattgcgag aataaagctt ctccttgtgc
    18601 caagacagcg gcagaaactt tcggctggtc tagataagtg ttcagccact tagcaagctc
    18661 atagaccact gtgatcccat ttgctgcatt aaaatcatcg tccatttctt cgatgaatcg
    18721 ctgttctaaa ttttctaaca aagcgatttt atccgcatca tctgccaaat tctctgaggc
    18781 agtttcttgg cggaatttca aattgtcaaa cgtattcttc agtcgttgta aattggtggc
    18841 tgcttctttc atcgtcgttt cactgtagcg gatcggacga cggtattgtg tggtcgccat
    18901 gaagaaacgc aagacttgtg gatcaacgtt tttgatcatt tcatgtacag tgatgaaatt
    18961 ccctagagat ttactcattt tttcatcgtc ttcgccaatc gttacatagc cattgtgcat
    19021 ccagtaatta gcgaattttt tccctgtttt cgcttcactt tgtgcgattt cattttcatg
    19081 atgaggaaat tctaaatctt gcccgccacc atggatatcg atcgtttctc ctaaatgttt
    19141 tgttgccata acggaacatt caatatgcca accgggacgt cctttgcccc atggtgaatc
    19201 ccaagaaatc tcgccaggtt ttgcttgttt ccataaagcg aaatctaatg ggtcttcttt
    19261 ttgctgctgt tcttcccctg tacgctgact tgctccgact tccagttcat cgatggattg
    19321 gtcacttaat ttcccataat tttcaaattt acgggtacgg taatacacat cacctgaaga
    19381 ttcataagca tagccttttt caaccaacac ttgaataaat gctaaaatat ctggcatatg
    19441 atccatgact cttggatgca gtgtagcagg ttcgacattc aacgctttcg tatcttcttc
    19501 aaatgctcgg atgaaacgat cagcaacttc tggtgctgta atacctaatt cgtttgctgt
    19561 acggatgatt ttatcgtcta catccgtgaa attcgatacg aaatcgactt tatagcctcg
    19621 atattcaaaa tagcgtcgaa ttgtatcgaa agcaatggtg ctacgagcat ttccgatatg
    19681 gatataatta tacacagtcg gtccgcagac atacataccg acatgtccct tttgtatagg
    19741 gatgaattct tctttttttc tggtcaatgt attgtagatt tttatcattc tgatctcctc
    19801 actttttctc ctttgattcg aacgactttt gctggtactc ctaccgctgt agcgtcgtct
    19861 ggaacatctt tcaacacaac ggcacctgca ccgattttgg cgttttttcc gatcgttacc
    19921 ggtcctaata tctgcgcatt cgcagagagc atggctcctt gtttaacagt tgggtgtctt
    19981 ttacccgttt cttttcctgt gccgcctaat gttacgccat ggaataaaat cacatcgtcc
    20041 tcgatttctg cggtttcgcc aatgacgatt cccattccat ggtcgatgaa cacgccagtt
    20101 ccaatcgttg ctcctggatg gatctcaaca ccagttagga aacgccagaa ttgagcattg
    20161 atcttcgcca ataaaaagag ccggtgctga tataaaaaat gagagaaacg atgccaaaac
    20221 aaggcatgaa aaccagggta tgtcaaaacg atctcagctg tcgaacgtgc agccggatcg
    20281 tttctctttg ctgcatcaat cgctcgggta aaccaactca tgactttcca ctcctttcgt
    20341 aagaatttta tgtaggaaat catcttctta taaagaaaag aagcgccttt taaataagtt
    20401 tccttatcaa aagacgcttt ccgcgtggtt ccacttttct tcataatcaa tgattatctc
    20461 tgacagctaa cgcctgttat acgttcttgg ctactttgtt tcctataaaa cagtttttcc
    20521 tgttttctgt gtttcgccaa gaccactccc agaggcattt cggcaaccat tcattgatac
    20581 cttgcaccac ccggtatctc tcttgtaatg atcagctgct tacttttttc tgttcatcgt
    20641 gtttagttca gtacttgatt taaatgtgct aatgattttt cacgaccaag caattcgatc
    20701 gtttcaggca attctggacc atgcatttgt cctgatacag ctacacggat tggcatgaaa
    20761 agattttttc cttttacgcc agtttctttt tgaacagctt tgatggcagc tttgacagtt
    20821 ggagcatcta ctacttccat ttgttctagc tgtgctttga atgcgtggag aacagtagga
    20881 actgtttcac cagcaagtac ttcttttgct gcttcattca atacaggatg ttcattgaaa
    20941 aatagcgtag acagatcaac gatttctgca gcatagctca tttgaggttg atacaggcta
    21001 acgatcttct tcacccattc gattgtcttt tcatctgggt tttctgctac acgtccatca
    21061 gcgattagat aaggcagaca catgtcagta aatgcatcta gatccattgc tttgatgtaa
    21121 tggttgttta cccattgcag ttttttcgca tcgaaagctg cgggtgattt acttaggcgt
    21181 tttggatcga acattttgat caattcttgg tgttcgaaga tttcgtcttc gcccacaggt
    21241 gaccagccca ataatgcaat aaagttgaac attgcatctg gtagatagcc taattcgcga
    21301 tattgttcga taaattgcaa gattgattcg tcacgtttgc ttagtttttt acctgtttca
    21361 ctgttgatga tcaatgtcat atgtccgaag gttggtggtg tccagccaaa tgcttcatag
    21421 atcatcagtt gttttggtgt attggcaata tggtcatctc cacgtaaaac gtgagtgatt
    21481 ttcatcaaat gatcatcgac cgcaacagca aaattataag ttggcatacc atcgcgtttt
    21541 tggatgacaa agtcgccacc gatattatcg gattcgaacg aaatagcgcc tttcaccata
    21601 tctgtaaatg cgtattctgt attgcgaggc acacggaaac gtacaacaga ttctagacct
    21661 tgtgcttctt tttctgcttg ttcttcaggt gtcaagttcg cacatgttcc tgcgtaatga
    21721 ggcatttctc ctcttgcgcg ttgtgcttcc cgttctgctt ctaattcttc ttctgtgcaa
    21781 tagcatttgt atgctcgatt gctagcaagc agctgatcaa tcagcggttg atagatttct
    21841 ttacgttcag attgacggta agggccgtat tctccaggat tttcaggaga ttcatcccaa
    21901 ttcattccca accaagcaag gttttccaac tggctttttt cgccatcttc gatattacgt
    21961 ttttggtcag tatcctcaat acgaataata aagtctccat cattgtggcg ggcaaacaaa
    22021 taattgaata atgcagttct tgcatttcca atatgcaggt gcccagttgg acttggtgca
    22081 tagcgtacgc gtactttcgt cattttcttc ctcttctttc aaactagact ttgactaaca
    22141 gcaaaaattc cgtcagttac ggcttctcaa atatcattta tttaaagcca ctcagcacaa
    22201 attttacctt ttctaaggta aatagtaaag ccaactggtt gaatttcctt accagttggc
    22261 tgaatttctt ttttattttg attgtttagt ctttttctct gtctcttttt tattgtcgat
    22321 tcctcgtcct gcatgtgcag gtttagcaaa gatcatgcgt ccagcagctg tttgaagggc
    22381 acttgttaca acgacttcga tacgttcatt catgtaatgc tgtccatctt ctacgacaac
    22441 catcgtgccg tcatcaaggt aggctactcc ttgttggcgt tctgtcccgg cttttactac
    22501 tagaacattc atcgtttcac cagggatgac gactggtttt acggcatttg ccaacgcatt
    22561 gatattaaga acaggtacat tttggaattc agagacttta ttcaagttat agtcattggt
    22621 tacaatgaca ccatctaaaa gcttagccaa tttgatcaat ttgctgtcta cttcactgat
    22681 atcttcaaag tctccatcgt acatttcgac agatataccc tcttcttttt gcaaggcatt
    22741 taggatatct aatccgcgac gaccacgcac acgtttcaaa ctatcccctg agtccgcgat
    22801 gtattgtagt tcgtaaagaa caaaattagg gatcatcaag gttccttcta agaatccagt
    22861 ctttgtaata tcataaatcc gaccgtcaat gatcacgctt gtatccaaga ttttatattt
    22921 atggaaattt tctcctgatt ttcgttctaa cagttgactc tcaacttgac tagctgtttc
    22981 ttgctcgatt tttttgcttc gtgaaccaaa aactttcttc cattcttcga ttcgtgtcgt
    23041 tcccattctc aatcccagat agcctaagat gatcatcagt aaaatcggca atacactatt
    23101 gacgaatgga atttccatat tgtaaagagg ggtcgagatc acaacaccta aagtcaatcc
    23161 gatgatcgca ccgatcgcac cgaagagaag ataagtcaag ctgacctcac ttagtttttg
    23221 ttctacttgt ttgataatag ctgagatatg tttggccaac agcaaagata ataaaaagaa
    23281 aataagtgcg cctaacaggc tgttggtaaa tgtattattc agccatccgt ttgactgttg
    23341 accgattgcc tgccaagctg agggtaaaaa tgaaataccc aagcttgcgc cgacaatgat
    23401 catcagcaaa gtaatgacac gtttttgcat cgtttttcct ccgtttcgac aatcatttat
    23461 tagttagcgg aacacacgct tcaacgtttc agccaaagtt gctactccaa ctatctcgat
    23521 tccttttggc gcttcccagt tacctaagtt gtttttaggt aggtatactt ttgtgaagcc
    23581 gagtttttgg acttctttga ctcgttgttc aatatgactg acacgtctga tttctccagt
    23641 cagtccaatt tcaccgatga aacattccgt tggctgtgtt cctttttctt tatagcttga
    23701 agcaatactg acagcgatag ctaaatcaat cgctggttca tttaatttta ccccacctgc
    23761 agcttttaag taagcatctt gattctgaag aagcaaccca gctctttttt ccagcactgc
    23821 catgatcaga gatacccgat tgaaatcaag acctgttgtt gttcgtttgg catttccgaa
    23881 catagtaggt gtgactaatg cttggatctc taccaaaatt ggacgtgtcc cttccattgc
    23941 cacaacgata gcagagccag ttgcaccgtc taggcgttct tctaagaaaa tctgtgaagg
    24001 attagcgact tcttccaatc catgctcatg catttcaaaa atcccaatct cattcgttga
    24061 gccaaatcgg ttttttactg ctcgcaaaat ccgaaaacta tgatgttgtt caccttcaaa
    24121 atataacacc gtatccacca tatgttctaa cattcttggg ccggcgatcg agccttcttt
    24181 ggtaacatga ccgacgataa aaatggctat cccatttgtt ttagcggttt tgagtagttc
    24241 tgcagtggtt tctcttactt ggctgacact tcctgccaca cttgtgatgt ctggctgcgt
    24301 catcgtttgg atggaatcaa tgataacata atcaggggag atatgttcga tggcacgact
    24361 gatttcgttc atatctgttt cagcgtatag ataaaattct gtgttgatac ttccgaggcg
    24421 ttccgcgcgt aattttatct gctctgcgct ttcttcgccg gatacatata agacttttcc
    24481 gcctatggct gctaattgct gagaaacttg taacagcaaa gtggatttcc cgatacctgg
    24541 atcgccacca atcaagacca tagaccctgg caccacacct ccgcctaaca cacggttcag
    24601 ttcttctaat tttgttttga tccgaggttc ttttttaggg acgacgtctg cgatttttgt
    24661 tggttttgct ttttctcctg tcaaacttgt ccgagttctg cggtccgttg tatcttgttc
    24721 gatttcttcg accatcgtat tccattttcc gcaatttggg cagcgtccca agaacttagg
    24781 cgacacatac ccgcaatttt ggcaaacaaa ttgtactttt gatttttttg ccattgtacc
    24841 gcctcctttt atcctaatca tgagacatta aatcaagctt aagtatcttt aacgttttat
    24901 ttactggaag aaccaaatcc gccttgacgt tgagcagtcg tttgatcttg atctgctttt
    24961 aaaaacggca aaaagatccc ttggccgata cgctcgcctt ttttgatcac tacatccttg
    25021 aaaccaaagt tggtgaattg gaacatgata tggccttcat ttgcctcatt attgtaataa
    25081 tcactgtcaa tcacacctac tccgtttgtc aacactaaaa accgcttcaa cggattgctt
    25141 gaacggttag caagctgcaa aaattcatct tctcccatgt aagctttgat tccagtaggc
    25201 acgagggttg gtttcaattg tttttcattt tctgatacat cttttttcaa tccatctgcg
    25261 actgtttcca accccttttt ccagcctgct ttccagatac ttggaatcac gacctcttct
    25321 gctgcttcaa aatcatagcc agcagcatga tgtgtagcgc gctgcggtag attgatattt
    25381 tgatctttat atttagaaat aacttcaaat cctctttttc tcaccggttt tcctccctaa
    25441 ataaactact cctattctaa catatccgag gctgtaaaag actttcttta caaattcttc
    25501 aagaaattct gctatcttaa agtaaaccat taattcgttc tttttccaaa cgactgcaag
    25561 ttgtacaaaa aaactatttt ttacatgact ttactcgtca aaacaagtag aatagaacga
    25621 aattaatgag tggatatttg attgaggtgt cacatgcgta aagaaattaa aaatgcaaaa
    25681 cgaattattg tcaaagtagg aacaagtaca ttgatttatc ccaatggaaa tatcaatctt
    25741 gcggcaatcg atcagttagc ttttgtcctt tctgatctta gaaatcgggg aaaggaagtg
    25801 atcttagtct catcaggagc tgtaggcgtt ggtatgcata aattacattt gtcagaacgt
    25861 ccgactttga ttcctgaaca gcaagctgcg gcggctgttg gtcaagccga actgatgaat
    25921 atctataatc aaagattcag tgtatataat caacagacgg cacagatcct cgtgacaaga
    25981 gacgtggtag attatcctga aagtcgtaaa aatgtgagga atacattgga acagcttttg
    26041 attatggaca cgatccctat cgtgaacgaa aacgatactg tagcagtgga tgaattagat
    26101 catctgacaa aatttggcga taatgatcag ctgtcagcca tcatggcaaa acttgtcgcg
    26161 gcagaccttt tgatcgtcct ttctgatatc gacgggtttt attcagataa tccgactaca
    26221 aatcccgagg ctcagatgta tcgctatatc gataaaatcg atgaaaagat tctggcacaa
    26281 gcaggtggtg ctggtagcac atttggaaca ggcggtatgc agagtaaatt aaaagccgct
    26341 tctaggatat ttgaagcaaa cagcgcaatg attttagcaa atggaaaaaa tccggcaatt
    26401 atttttgata ttcttgcagg aaaagaagta ggtactttat ttaaggagga cttgaaatga
    26461 ctgacttgat gcaattagga caacaggcaa aagatagcgc gcaacaacta gcacttatgc
    26521 ctacaaaccg caaaaatgag ctacttgttc aaatggcaca aaccatcaaa aagaatcaac
    26581 aagcgatcat tgaagcaaat cataaagact tagaaaaagc agtcgaaaac aatatcagtg
    26641 aaacgatgca agaccgcttg cagctgacac ctgagcgaat caatgccatg gctacagaga
    26701 tcgaaaaaat tgcttcgtta gacgatccta ttggaaaagt agatgagatg tggacgaaca
    26761 ccgacggatt gagaatcggc aaaaagcgcg tgccactagg cgtgatcggc atcatttacg
    26821 aatctcgtcc aaatgtcacg acagatgcag ctagtctagc attcaaatca gggaatgccg
    26881 ttatccttcg cggtggcaaa gaagcattct tctcgaatca attattagtc caattattgc
    26941 agcaagtact ggtcagtgct ggcgaatctc cttatgccat ccagtttgtc gatgatactt
    27001 ctcatgaaac tgcaggacag ttgatgcgat tgacagaata cttagatgtg ctgattcctc
    27061 gaggcggagc aaaattaatc caacgagtca aagaacaggc aacggtacct atcattgaaa
    27121 caggtacagg aaataatcac gtatatatcg ataaagaagc acagttatct atggcagtga
    27181 acatcatcgt caatgcaaaa gcttcccgtc catctgtctg taacgcggca gaaacattgc
    27241 tgatccattc tgagatcgca ccttttttct tgccagctat tgaaaaagaa ctggtagaac
    27301 acggggtctc tttacgtgcg gatgctcgtg cgttagaata tctagagacg gctgctttag
    27361 cagaagacac agattgggat actgaatatc tagattatat ccttgctgtc aaagtggtcg
    27421 attctttgga tgaagcaatt gcacatatca atcggcataa tactaaacat tcagaaacga
    27481 tcgtgacaga tagttatgcg gctagtcaac gtttcttaaa tgaagtggac gcggcggctg
    27541 tctatgtcaa tgcctccact cgttttacag acggatcggt atttggtttt ggtgcagaga
    27601 ttggtatctc tactcaaaag ttgcatgccc gtggtccaat ggggttaaat gaattaactt
    27661 ccacaaaata tatcatttat ggcgatggac aaatccgcga ataatgtttc gtgtgaaaca
    27721 ttattcccgc cttttgcctt ttttctgtaa aggttctatc caactagaaa ggtgtcaata
    27781 tgtttgaaat tttaatgatt ttttttattt accttatttc tctgaatatc gctgcttttc
    27841 tgggagtcgg tattttatcc cttttctttc aattcaaaaa acgaagcata gggagtcaac
    27901 gggaaaaatg gtcgcagtat tttgacaaaa tcgggcaaaa agggctggtt acacgcttgc
    27961 acattagtta catggttgca ttatgcttat tagcgacaat caattactat tcttttttcg
    28021 atcattcaat cgcttatacg attacgctac tgattgctgg gatttttcac ttatcttata
    28081 aatatcagct gaataaaaac cacttgaatc ggactttccg ctaaaaaata tccgtacgat
    28141 aagtagagtg ccttgaagat cggcaaaaaa ctgccggact ttttagaggc tgtgacaaag
    28201 attttgtcac agcctctttt tccatataaa cggcgttcgc ctaccagctg cctggtaaat
    28261 ctgtttcctc ttcgttaaga agcgaaaatc cgtcaaatgt ttcgccatca taataacgaa
    28321 gcagctcatc catggacaag ttcatcgttt tttctttttc ttcttccgct gattcagcca
    28381 gtagctgttc ccgatacacc tgatacatac cagctatttt ttgctgcatc gcttttctag
    28441 tttcaccttc cgcattgatt attggcaatt ctttgatata gccacgataa atcaaccgat
    28501 catctgaaaa ttcaatgggt aattcaacga gtgtcaaccc gacttcctga aaatattctt
    28561 ccagcatcta ttatcccccc attctttttc ttaaatgaat tttagcttac ctactgaaaa
    28621 ttttccagtt tcattatttt gagaaaaaaa gggaatgtga caaaaatcgt gtcacatccc
    28681 ctttttccga ataaatggtg ttcttctgcc aactcctcaa tcataagtca caatattcaa
    28741 caaacatgag aaactgtttt ttgaaattgt tccttgtgat tcagagttaa acgacagtct
    28801 cacaacctct tggtaaacgg cgttcaatca gctgaccctc ggtattagct caaaaaacgg
    28861 taaaatatga gaagctattt ttccgttttg tttctctaat acttaggata aaacgctgat
    28921 tttacaacct ctgttaaacg gtgttcaatc agctgtcctt cggtattagc tcaaaaaacg
    28981 gcaaaatatg aggaactatt tttccgtttg tttctctaat actcaggaca aaacgctgat
    29041 tttacaacct cctgataaac ggtgttcaaa agcttgcatc tgcggtaata agcccaacca
    29101 aacgaaaaat atgagaagct atttttgttt ggctgttctt atgacttgat gcaggacagc
    29161 tttttcacaa cctctgattt cttattcttc ttcatttgta gcaaaataat tacggtgata
    29221 aatgatacaa catacactga taagaacaat ggcacctgca aaataaaacg catagctttc
    29281 accaatatgt tcaccaatca cacctgcaat aaatggtgct aaagctccac cgaaaaagcg
    29341 gacaaaacta tagctggacg aagcaacgtt tcgttgcaga cctggtattt ccatagcaat
    29401 cgtcgtcaac agtgtattga taattccttg gaaaaatcct gctaacacaa tgctgatcgg
    29461 tacaaggact ggcatagaag ttccagctcc taataacagc aaacaaataa tgaataagac
    29521 aaatgctgct aaaattgtcc gataagtcgt aaacttccgc tcaagagccg gtgcaacgaa
    29581 aatcgaagca atagccagaa gcacacccca gccaaaaaat acaaaaccga cttctaattc
    29641 agaatagcca atcaataaaa atggcgcata cgcaagtaaa gtaaagaatc cgaaattata
    29701 taacaatgcg attagtccaa ctgaacgaag tttatgattg cttaacgctt tgacaccttc
    29761 ccaaaggcct acttttctct ttggtttctc agtttcaggt aaaagtaccg ctagggctat
    29821 aaaaccgcaa aacatcaaaa atgctacccc aaaaaatgga taacgccatg aaccactgcc
    29881 caagacccca ccgactagag ggccaacagc cattccgcag ccaagtgctg cttcatacat
    29941 catgattgct ttttctgtgt taccaactaa aatactgacg atcgttgcta aagcagtaga
    30001 gacaaataaa gcattcccga caccccagcc ggcacgtaac ccaatcagca ttccgatatt
    30061 cccagataat ccgccaagac cagcgaatac aacgatgata aacaatcctg tcatcaatgt
    30121 ttttttcaaa ccgatccgac tagataagaa acctgtaaat agcatcacca caccagtgac
    30181 taacatgtaa ctagtaaata acagggtcgt ttcagctggc gtagcgttta actgtgcagc
    30241 aatcgttttt aagattgggt ctactagacc cactcccata aaagcgatca tacaggtagc
    30301 tgctacaact agcgctgaac ttttcccctt tgtttcttca tttgtttgtt cttgtatatc
    30361 tatttgattc tccaatattt ccatcccttt ctctctatta gcatcataca tgatttattt
    30421 tacatgtcaa tatgcacatg taaaacgtgg aaactttact gtgatataat aaagaaaata
    30481 agacgagagg taggattttt atgaatactt tagcctatat attattatgc gtgcttttat
    30541 ccaaaccacg ttctgggtat gaattgaagc agctgatcac aattttttgg gaagcccatc
    30601 acagtcagat ctatacaacg cttgccaagt tagaccagca gggttatgtg accgttttgg
    30661 acaaagacga gcactctcaa aaaaagatct accacttgac tgatgaagga cgtgctcttg
    30721 tagaggaatg gatcaaagag gaaactcccg ctcctacgca aaaagatgaa tttctagcaa
    30781 aaatctatgc tttttcgacg ctcgataaaa caacagctac tggactgcta ttcactagag
    30841 aacaacagct gaacaaaatc atgcaaaaaa atcaaaaaaa attgaatgac ctctcgctag
    30901 acaagaaaca agaatttgga cgttatgttg tgatccagcg cagaattatg ctttgtcagc
    30961 aggaactcca atggtgtcag caagtaagag aacaaatgca gcagtttttt gagtaaggga
    31021 taaacagaac ctttctagaa atatctattg aagagcaaat tatcttcaat aaaagagcaa
    31081 tacagttgga aaagaaaaag cgatccgaac gacaggccaa aacctatcgt tcggatcact
    31141 atttttctag aactgcattc aagagactat gacaaaaagc atgccaaggc cttttttcaa
    31201 ataacagtgt tccatcattc attactttga attgctttta gcacacttct tgaaagcggg
    31261 ccaacgatga tcaactgcaa cggtaaagcg gcaatcacat tcattttcca agcattcata
    31321 taagcagaga ataattgatc aggtattcct tgttccatga tcacaccgaa taaagacata
    31381 catgtcacca ttcctaaaac catcaagcat gaaatagtta agatcatttg gaccttgctt
    31441 tctggattta ctggtagttt aaaggcaatt tttttcgcca gcacaccgat gatgaagaca
    31501 tccaaaatga aagccaccac gaatccaggc actagaccaa tcaacaaact tgataaatga
    31561 agatcttgat gcagccataa attatacgcg ctcatcccca agaccatcaa aaaacacatc
    31621 attgttgtaa aaattattcc ttctttttta tttgtaggca atctgtacca ctcctttaaa
    31681 tttgtcacga aaatcatcgt agcacattta aaaatttctc gtaagtactt tttttatttt
    31741 ctgccctttt atttcataaa cttcatcagc cgtacgctcg acaaagtaag aatcatgaga
    31801 agtaaacaaa atcgttcccg gatatatttg cattagcttc tctaaagctt cgatcgttgc
    31861 catatcaata aaattagtcg gttcatctaa cagcaacaca ttacttggct tagtaaaaag
    31921 caaagcaatc gtcagacggg tcgcttctcc cccacttaat gtacataacg gtttcgtcaa
    31981 agtttcctga gcaaacccta agtgattcag caaactacgg acaagcgatt ctgaaaactc
    32041 tgtttgtctc atgacaaaac gaatgatgga ttcttcagat gtcatttgat aacccttttg
    32101 ctgatatact tgaaagctta ctttcggaga gagaaggatt ccttctcctt gtttgcggat
    32161 atgttctaat aaggttgtct tacctgaacc attttcaccg acaatcgcga tttttttacc
    32221 taaagaaaac tgaaaatcac catttaccag taatgttctc ccacttcttt ccaattggac
    32281 attttgtccc atgatgggat agcggctgtg gagttcaaga gattttggta ctggaaaacg
    32341 gatttgtttc atctgctgcg gcttggttgt ttctcctatt ttttggagtc ttctctctaa
    32401 tgttttcgca ttcttttgga tggctttttg tacactgtct ttttgtttag aggaagacaa
    32461 ccgatccagt ctgatttgtt gctttttctt tttactggac actttttcca acttttccgc
    32521 ttgttttttc ttttcttgga tcgatttttc caatcgtttt ttctctttca tgatcgactc
    32581 tgcttctcgt agctgagtct ttttctccaa ttccttttga gtgagatagg cactataatt
    32641 ccccgaaaac tctcggattt ctccgtcttt tacttcccag attttcgatg ctagctcatc
    32701 tagaaaatag cgatcatgac taacaaaaac cagcgtacca taataataac gaagctcttc
    32761 aatcagtcga tcaatacttt ttctatctag atgggttgtt ggctcgtcta gaagcatccc
    32821 ttgttcataa gaagaaagaa gacgtatcaa ctcgatcttt cgttcttccc ctccgctcca
    32881 tttccgctct gaagtttctt ccagtcttaa ctggaaatga ctggctaatt ccaaatttag
    32941 gtcctttgct tcagcgagat aggttaattg aggcaggtag ttgaaggtga tctctgtttg
    33001 gattttccca tgatcgggaa aaagttcacc ggctattagt ttgagcaaag tcgatttacc
    33061 gacaccattt tctcctatga tccctatacg atcttgctga tagacacgaa gagaatcaat
    33121 agatagttct agctggttgc caaagctgac ttgtagattt gttatattta ctgctaaatt
    33181 ttccatatgt tttacactcc tatttcatgt aaaacaacaa ttatcgatac taaaaaagag
    33241 cagaaaaatc tgtttgcaaa aaaactgcat cacatctttt cctactctga acgaaataac
    33301 tatgactaaa tcaccaaatc tgtcatagct ttcacctatt ctactgtcgt tcgaaaaaat
    33361 ggacatactc catccatgaa attgttgttt atacatgtct tttgaattta tttagttgtt
    33421 attcaaaagt tcgaaacgta atttcatcga tgcagtcatt tttccgacct ccttttttat
    33481 taggttacca cacacgattc aaaaaaagca atagctactg aatagtaact gaaaaccttt
    33541 tattcgaaca tagtgatctt ttctggtgct aaacgaacag actcgtaacc ttcttctacc
    33601 gcttttccta ttgataaaat aataactgga acataacggt ctttatccaa accaaatgcc
    33661 tctgccaact gatctgcttc gaatccacca attggatttg tttcatagcc atgatcacgc
    33721 gcaactaaca tgaattgcat agccgctaag ctagaatcga ctttgactac atcgttcatt
    33781 tcttctcttg aaaaactctt gtaataagga atgatcgcag ctaattgttg atctctgact
    33841 tcttttggca tcttccctga ttcgtaagct tggttgtaga tttcttcgcc atattcgtaa
    33901 cattgcatat cgccaaaaat catgaccatt gcagaagaag tatcattttg tctcgtattg
    33961 aaacggatca aaggacgcaa gatttctttt gcttcctcgc tttcagcgac cacaaagcgc
    34021 catggtttca tattgacaga agaaggtgca gtcgtcgctt ctttgatcat ttccagcatt
    34081 tcttcgtgtg ggattttgta tgtttcatca tatacgcgaa cggatcttct accagtcata
    34141 attgttgaaa aatcattttt ttgttccatt attattaacc tctttcgttt ctgttgttat
    34201 attgcaaagt tgggtattca atttatctaa aagtgttaga aattgtttca tttctacatc
    34261 gctgaaagcc ggggatagca cggaacctgt atctttatgg tccatttcac aatgcttgat
    34321 tttcttctgg gctttttctg ttagcgctac atacacttcc cggttgttct gaggatttct
    34381 ctgacgcgaa acatagccat ttgcttccaa taccttcaga tgacgagtaa ctgctccttg
    34441 atcaatctgc aacttttctt gcaaactgct ttgtagcaat ggattttctt ctttcagtct
    34501 catcatgatc tcgtatctag taagacttat accgatattt tcttcaaaga tctggacgat
    34561 tttttgatcg gctaatttga tttgatataa aaggtgacta atctcttcta aagacataat
    34621 ttcctccttt ctaataattg actcgtcaac gattgacttg tcaataatag aataaaaaaa
    34681 gaaaaaagtc aaagaatcag ctgtgtaata aaaaaaacgc tctcaataga aaagtataga
    34741 gaaaagaaat tttttgtaga atgaacggaa aaaatgataa agaaagcaca acgctaaaac
    34801 caagattatt gattcttatc aaactataga agatagctct tctgcagtag ataattttac
    34861 accgccaaaa atttccgaaa tcgccttttg caagaatccc gtcgaatatt agaaattgga
    34921 tcagtatgct taaacagtaa taaaaaagtt atgacaagat gttgtcacaa ccttttttga
    34981 gtaaacggtg ttcaaaaact tgcatctgcg gtaataagtt caaccaaaca aaaaacatga
    35041 aaatctgttt ttgtttggct gttcttacta cttgatgcag ggcagctttt tcactacctc
    35101 tttttaataa ccataaaatc ctattccttc atatctccag cccatcggtg ttagccaatc
    35161 tctttcctta gcactcaagg tatagtgatg ggtaccagtt ttttcatttg gattaaatag
    35221 tcgataaatt ggcttggaac caccagaata aaaacttaga ccttcataac gccaaccgat
    35281 ttttgttaaa gagttgatct cagattgtgc cattgtgtaa tgatggtctc cactgtaagg
    35341 attgtataaa cggtagacag gttgtccgct gtttggtgct tgccaagcaa tcccttcata
    35401 ttcccagcct acagttggca gataattttt ttctccggca ttcaatgtat ataaatgttc
    35461 tcctgtatga cggttataca gacgatacat cggagaagtt gaagttggtg ctgtatcata
    35521 ctgtgtcaga ttataggttt cgattacatt gttcaatgta gaaccataat taggcgcagt
    35581 ggcataacga cctgttagcc aagccgtagc atctttgtaa gagttcgtat tgcttttcca
    35641 tgcaccggaa tagtagtaaa cgcctggctg aaaagaagtc gtcttcaata ccgttacgtt
    35701 atcttgcaat gattctttgt acgaaggata ttttctaaaa gcggcattca ccgtaatcca
    35761 ttgcccattc acatattccg aagtaggcat cgtaacagat tggccattat agcttccttt
    35821 aatcccaaat agattgtaat taggggcaga agccaaagta ctgttccccc agccactttc
    35881 aacgatggct tgagcaatca tgactgatgc atataagtca ttggcacttg ccaaactttg
    35941 tgcagttgag gcaatactat caatgaacgc ttgttgagaa ttagcagatg agcgagcata
    36001 aaaagcatag ccagtatagt tttcgaattc agacaaatct gcttgatggg tatgtgtcaa
    36061 tgtttgccca tctgtcaatt ggttcgtgat ttcttctgca ttctctggat tgttctgagc
    36121 ttctgctgat gtactgtctt cggtttgtgt ttgttctgtt gtttcagttg ttggttcttc
    36181 ggttgaaatt gtattttctg agacagtagc tgactgcaaa gtactttctg tatcgttttc
    36241 aattcctgtt tctgaagaac tcgatgtctg agttgcgaca gtttctgtcg attcttcagc
    36301 tagcactgtt gtgttggcaa ctgctagatt tcctaaaaga ataacgctaa ataataaatt
    36361 ctttgctttc atcttttttc tcctttgaat tatgaaactt ttcctaaata acagtgtaac
    36421 taaaaaagta aaagcaagtc aatgtccata tttaaataac atttgtttct ttttaataaa
    36481 tttgcttttt ctgcataaaa agacgtaaat gaagaatatt cttctccatt cacgccttaa
    36541 attgatggtc gtttgaataa ctatttaaca agtaattctc tctggatatt atccgttatg
    36601 gattatactt cctgtgtcgt cggacggata atcatgttgc tgacagacat ccggtctggc
    36661 gtttctatcg caaatgctac agcagaagct atatcttcag atgttaatcc ccattctttt
    36721 tgcgcttcat gaagttcaag agcagccttt ttgtcgctga tggtttgata taattcagtt
    36781 tgaacagctc ctggagagat gatcgtagat ttgatgttat tttgccgttg ctcttgacgt
    36841 aatccttcca tgattgcacg gacagcaaat tttgttccgc aataaacggc ggaatcagga
    36901 taaacaacat gtcccgccac agaatcagtt gaaatgatat gcccagattt ttgtttttcc
    36961 atgattggaa gtacggcgga aattccattt aacacaccca tgatgttgat atcaagcata
    37021 ttttgccatt catcacggtg accttcaact aatggagcgg ttggcatgat tcctgcatta
    37081 ttgtacaata catcgattcg accgaacttt tccatcgcta aatcgataac tgcttggact
    37141 tgtgcaaaat ctcttacatc tgctgtttga atatatagct cagcttctgg taaagattct
    37201 tttattgctt ttaggcgatc ttctcggcgt gcagctataa ctagtttagc ccctttttct
    37261 gctaatagct tcgttgttgc ttcaccgatt ccgcttgaag cgcccatgat gacaatcact
    37321 ttttctgata aatcattcat tctattctcc tcctgttttg aaaaatctct tgcttatact
    37381 cactatctct tatttcatct tactgttcaa ataatccgtt cgacaggttt gacgattttg
    37441 gcagggattc ctgcaacgat ggtattgtct gggacatctt tcgtaacagt tgcattcgct
    37501 gcaacaatgg cattctcgcc aatcgttacg cctggcagaa tagttgctcc tgctccgatc
    37561 caagcatttt tcttgatgtg gattggcttt actgacaagc ctcttcgttc ttttggatcg
    37621 gttagatggt tcaccgtgat gaggttgaca cgaggaccaa tcaatactga atcttctaaa
    37681 cagatgccac ctaagtcaac gaacatcact tgacgattga taaagacatt tttccctata
    37741 ctaatatgtt tgccgaaatc tgtataaaaa ggcagcagaa cagtcaccgt atcatctact
    37801 ttgtcactaa tgatattttc tagccgtttg cggatatctg ctggttcatg gtacccagta
    37861 ttcagcgctg ctaaaggctc ttcgttttcc gcttggatct gatggatcac ttcaaagagc
    37921 ggatccgtta ctcgaatctc tttattgacg atttgttttt cgatgaggct tgtcattttg
    37981 ctccctcctt ttatccttac tttataacgg acggcttatg atgtctaata cttataaaac
    38041 atacttaact attcctttta actatactta gtggtatgct tttttaaaaa caggagggaa
    38101 aaaatggata ttcgtatact gacgtatttt ttagcagtag ctagagaaaa aaatatcagc
    38161 cgagcagcaa aatctctgca tatcacgcaa cctactctct caaaacaact gaaagattta
    38221 gaagaagaac ttggtgtgac acttttcact cgaggctccc gagagataca gttgacggat
    38281 tctggtcgat atctttattc aaaaggaaaa gaaatcttgt cgttggtaga aaaaacggcg
    38341 aacaatttaa caaatgacgc aatcatctct ggagaaatct acattggcgg aggcgaaact
    38401 aaagcaatct ctttcatttc tgatagcctt cattcgttga tcaaggcgca tcctgatatc
    38461 cagtttcatt tatatagcgg gaatgcagat gatatcatag acaaactgga tagcggatta
    38521 cttgattttg gactcgtaat cgaaccgaca aacaaacaaa aataccagtc atttcgactg
    38581 ccagcccaag atcgttgggg acttttgata catcggaatc atcctttagc aaagcaaaca
    38641 gtcatccatc cgcaggaact gaaaactgtt cctttgatca tttcccggca aacgtcagtg
    38701 gacagtcaac tagctgaatg gctaggtttc agcatagatc agttatcgat cgtcggaaca
    38761 tataacttat tgtacaatgc ttctttgatg gtcgataacg ggacttttgg tgcattagct
    38821 ctagatggga tcatcaatac ttatgggacg gaaaaagtat tcatcccact cgatccgcca
    38881 ttacaagctt cactgaatct catttggaaa aaagaccagc ctctttctcc tgctgctcgt
    38941 aaatttttag atgtcctaac ggagaaatac agttaagtag agtttttttc cttcgctttc
    39001 agctttttaa caacgaataa gttaagcaaa agatccaatg attaaataac ataagtttct
    39061 taatttgttc cttcctttta tttctgataa ttccagtaaa ttactataat tataaaaaaa
    39121 gaattgacaa aacataactg tctatataaa attgattacg ttaacaataa ttcgtttcat
    39181 gtgactagat catactaggc atgaaaaatc tctgaggaac atggtttccc taggaggttt
    39241 ttcgtgtcta tttttgtttg gccgcaatca actggtctag caaacgtgaa aaccaaatct
    39301 tcaaaaaggg tgcgcgcatg aaaatcaaaa agatctttaa tcaaaatgca gtattggtag
    39361 atgacggtgg acaggaaaaa gtagctattg gaaaagggat tggatttgac agaaaaagaa
    39421 atgatttgat ttttgatcgt gatgtcgaac ggatatttgt catggaacca gaaggtcaaa
    39481 tcaagttgca aaatttactt aatcagattg atgaacgctt tttatttgca gcagaaacga
    39541 tcattgatca tgcggaaact gttttgatgg aaaagttgaa tgaacatgta ttgatcgctt
    39601 tgaccgacca cattgctttt tctgcggaga acattaataa cggtatcgtc atccgcaaca
    39661 aacttttaag agaaatcgaa gtgctttaca gtgatgaatt cagtattgct caatgggcag
    39721 tagagtatct gacaaaagag cttgggattc catatactta tgacgaagca ggatatatcg
    39781 caatccatat tcacagcgga cgaagcggtc ggaacaataa ccatcgcagt atccgtgaag
    39841 tgacgattgt atctgacatc atccatttag tggaatcaga attggaaaca gatatccatt
    39901 cagaaccatt ttcactgaat tattcaagac tggtgaatca tttacgactg cttctacaac
    39961 gaacccatgc gcaacaatat gctgttttag acacggaaat cgtagaaatg gtcaaacgaa
    40021 aatacccaga aagctataag atttctaaaa aaatccgtgt gttgctgacc aaagaatacc
    40081 agctcgcgat cacgaaagaa gaactgggtt acttggccat ccatatcgag cgtttaagaa
    40141 gcgcaatcgt acaaacaaat gttaacaatc atgaggagga aaaaaattga aagcatatct
    40201 acaacgaatg ggacgttccc ttcaacttcc agttgcggtc ttacctgctg ctgctttact
    40261 agtagggatc ggaaactggt gggcatctta tagtaacgac attgtcgctc attttttaca
    40321 agctggcggt aacgcagttt taggtcaatt acctttgctg tttgccgttg ggttagcact
    40381 agggatgtca gtggacaaat caggtgctgc tgccttggca ggtttggtcg catttgaagt
    40441 tccagtaaat gttttaaaaa ctgattcagt agctacttta ttgaatatca aaccagaatc
    40501 agttgatcca tcttttgctc agatcagtaa cgtatttatt ggtattattg ccggattgat
    40561 tgcggcagct ctttataatc gtttccacga agcaaaatta ccgatggctt tatctttctt
    40621 tagcggcaag cgtctagtgc caatcttggc tgcctttgtc atgttgctga tcagtgcagt
    40681 gctactgctc gtatggcctc cggtttactc tgctttagtt tcttttgcta aattcatttt
    40741 gagtttaggc tggggtggtg caggtcttta tggcttcttc aaccgtttac tgatccctac
    40801 aggattgcat caagcaatga actccgtgtt ctggtttgac gtagcaggaa tcaatgatat
    40861 tggtaaattt ttggcttctg aaggaacaaa aggcgtaaca ggaatgtatc aagctggatt
    40921 tttcccaatc atgatgttcg ggcttccagc aggagcatat gctatttaca gaaatgcacg
    40981 tccagaaaac aaagcaaaaa cagcttcctt gatgttagca gcagcttttg cttcattctt
    41041 tactggtgta acagaaccac ttgaattctc atttatgttc gttgcttggc cattatatgt
    41101 cttgcatgca attttcactg ggatttcttt agcagtgagt gcattcttcc attggacagc
    41161 aggatttgcc tttagtgccg gatttgtcga ttacttccta tctttgaaaa atccgattgc
    41221 gaatcagcca ttgatgttga tcgtccaagg attagtaaca gcagtgattt actactttgg
    41281 ctttaacttt gctatcaaaa aattccatct gatgactcct ggccgcgagg aagcagatct
    41341 ttcagatgac gatactgcaa cgactaattc agacaataaa tatgcggcac aagcaagcaa
    41401 aatctacgct gcattaggcg gagccgacaa tgtaacttcg atcgataact gtactacacg
    41461 ccttcgtttg caagtcaaag atacaggcct gatcgatcaa aataaaatca aagccactgg
    41521 cgtacctggt atgaagatca tcgacggaaa aaatgcgcaa gtcatcgtag gtacagaagt
    41581 acaattcgtt gcagatgaga tggcaaaact acatggtggt gctgcaacac gacctgcaca
    41641 aacaaacacc gtggttaaaa cagaaacatt tgccggtgga acgatcgaac aagatattta
    41701 cgctatcgca aatgggaaac tgattccaat cactgaagtt tcagatgatg tattcgcgga
    41761 aaaaatgatg ggtgacggct atgctgtatt accagaaaat ggagaaatct tctctccaat
    41821 cgctggtacg atcacaaaca ttttcccaac gaaacatgct gtcggaatcc aaacagatgc
    41881 tggtatcgaa gtcttgcttc atatgggcat caacactgtt gacttaaaag gcgaaccatt
    41941 cactttatat gtaaaagaag gtcaaaaagt cgcaagaggt caattgattg ctttagttga
    42001 tcttgccgct attcaatctg caggcaaaaa cacggatatg gtcgttgtat tcacaaatgg
    42061 cgacaaagtc caaagcttag aaatcgaacc cgcacgtgat gtaaaagcaa atgataaaat
    42121 cggctctgtt tccaacaaag cctgagccat cgtcttcccc agactttttt atcgataaac
    42181 aaacaggagc tgagacaaaa atcttgtctc agctcctttt tccgaataaa aacggcgttc
    42241 tcctgccaac tccgcggtaa taagtccagc caaacgtaac atatgaagag ctatttttta
    42301 tttgactctt cttattactt gatgcagaac agctttttta caacctctga taaacggtgt
    42361 tcaaaaacca gtttatcggt atgaagtcaa atttatgcaa aaatgtggaa agccattttc
    42421 acaaaatttt tcttcatact caaaactaat cggctttttc acaacctctt tatacattat
    42481 tcctgatcca atatttcttt taatgctgct tcaagagaag ttaatggttt tcccaatact
    42541 ttttctaaat catcagagga aatagctaac tgatcctttt taatatcatg ttggatcgct
    42601 aaaaacatgt cagctgcttc ttgagggaat ccggcttctt ttagattttc gacaaacgct
    42661 gcatcacttg cttcaataat ttctaacttt ttacctgtcg cacgctctaa tgccttggct
    42721 aattcttcat agtcagttag attgccggat aattctaaga tttctggaga gtcactttct
    42781 actagtactt ttgctgcagc ttctgcatat tcacgtttca atgcccaacc tgtttttcca
    42841 tttcctgcag cataaacgaa ttgaccagtt tttaaagcct gcccaatgat aggtaattca
    42901 ttctctacat accaattatt ccgtaaaaat gtatgggaaa tacctgaatc cttaatcatt
    42961 ttttcagtga attggtgatc tggcgctaat acactgatcg agtgatcggc atctgcaaaa
    43021 cttgtataag cgatataaga taccccagct gcttgtgctg ccttcactac attcttatgt
    43081 tcggcttgac gatttcctgg aacgccagag acaaaaagca atcgatctat tcctttcaac
    43141 gcttctttca ttgagtctaa atcatcataa tcaccaatac gtatattaaa tccagcttct
    43201 ttcagtgctt ctcctttttc ttcactacgt actagtacat agatttcatt ttgtggaacc
    43261 atctttttta agaattccag cgcataacca ccaaaacctc cagttgctcc tgttactaaa
    43321 taatgcatat ttgccactcc ctattctttt tatttgttaa gatttacagc tgtacattta
    43381 taaattacac ctttatcata aagagtgtgg taagataagt caagcaaaaa aaatgaaagg
    43441 atgacaactg atgaagtatt ctctgcaatt tagtgatgca atccatgtac ttgcctatat
    43501 tgaaatgttt caagataccc gatcactttc tagtgagatg attgccagta gtatcgaaac
    43561 aaatgcagca aatgtacgta aaatcatgag tcagctcaaa aaatccggtt tgatctttac
    43621 aaaaaccgga aaaccgcagc cttcccttgc caaaagtcca gatcagattt ctttactaga
    43681 tgtctatcgc agcatcgaag gtaatacaca gcttatccaa gtcgatccaa aaacaaatcc
    43741 aaattgcgtc gttggtgcga atatccaaga agctttagaa gaaagctacg ctcggctgca
    43801 gaaaaaggca gaagaagaaa tggctaagat cacacttgat agtctgatac ataaaatagc
    43861 cgttttggaa aaagaaaaaa gaccagaaaa cacaggcctc gtagagagat ttttataatt
    43921 ctctctactg tgcctgcatt tttctgatcg gaatcgtcta cataaattct tttattctcc
    43981 gggggacaaa agccttgtca tgcccctttt tcctctctaa tgctcaggtc aaagcgctga
    44041 agtcatgacg tcttgataaa cggtgttcaa gtagctgacc ctcggcatta gttctgaaaa
    44101 atgcaaaata tggaaagcta tttcccattt ttcctctcta atgctcaggt caaagcgcta
    44161 ctttcacgac ctctatatta tcatggttta ttgactaccg gtatccagat ttctcccata
    44221 tatttcccgt tggtaactcc catttcgact acagcatttg gacccccaac ataggcgtaa
    44281 tcttctactt ctcttaaaac atgcccaaag gccgtattcg tcaaatcatt tcttaattct
    44341 tcaggcgatt ctgctaaagc gattacaaca agatacgttc cttttgggaa tgggatgatt
    44401 cgggtagtgt ttggaacatc ctgactcgtt tgaacaccga catagtacat cattttcccg
    44461 ttatagggtt cgttgactgt aaagaaataa tgattttcag cagcttgttt caatgcatct
    44521 attgttccat taccgataac tttttgctca aatgcttgtt tctctgcagc caaaccgaca
    44581 aaatccgtat tgctactctt caattccaca ccaatacccg tcaccgtaaa atctggtttt
    44641 tcttcgatcc tatactttgc catttctccc accctttcta ttatattaag ttcatcttac
    44701 ataaaattaa agggaaatga cacgattacg ctcttttttc tagggatatt tttatttttt
    44761 aactattttt ttgctttcac tgcaatccaa atctcactgt aaacattact gaaatcctct
    44821 ccccattttg taaaagagat ctcgggagct tcgactaact cataatctga agaaggaagc
    44881 cattctgcat agattttccc ccaagtatct tgtaaagttt ggggaaatgg ccctttatta
    44941 ggaaaaatgg cccaagtgtg ttcttctata gcaagcattt ctaaatcagc gtacggattt
    45001 tcttttgtcg tagcaaagcc aatcatgtgc atcaaactgc ccttttcttc caagcgggct
    45061 ccatcaaatc cataagaaac gttgattact tgatttggat ataaatctcc taacttatgc
    45121 atctcttccc tttgagtctc cgtgatcgtc tgcgctaact tttgtatttc ttgattgact
    45181 ccttcaaatt tgacaggaac ttttttcgcc acacctgcca cataaaactg ttctttcttt
    45241 tcgatacgaa attccatcga ttttcctcct ctgacatcaa taaaaaatgt caagcgtgga
    45301 aaagattttt gtatgccgta tctgctgatt tctgaaggca gatatcctga ccaatcacga
    45361 aaagctcttg aaaaaccttc gactgtttca tatccatatc taaacgccgt ttctgtcact
    45421 cttgatccat ctaggagatc gcgattagcc aaagccaatc tacggttttt gatatagtcc
    45481 attagcgaca tccctgcaat aaacgtaaag atgcgtttta attgatattc agagattcct
    45541 gtattttcgg ctattatttt ataggaaagt ccttctgtca aatgttcttc gatgtaatcg
    45601 atcaattggt tcagatcttt aagcatattt atcttcctcc ttgcttgcct ttttatcata
    45661 ttatcaaaga aagtcaatta cttgatgaat caggaaccaa tttagtcaga tgtagaaaat
    45721 gttgtatgga tctaaagaaa attctcaagc agcgtttcgt aacggataat tggtattatc
    45781 tgcctatcta tgttttacct ttagaaaaat aaattttggg gaagacacat tctaagtaaa
    45841 caaagcggta aaaatatctt ttattccctt agttaatccg cttactacct actgtacaaa
    45901 gcaaattgtt gttcaaactg ctgatcagaa cgctgcttgg atttttcttt gcgatacatc
    45961 atacctcttt tatcaattgg aacaattatt tatttctaac aaaatcactt catttttgtc
    46021 agataaataa a
//
LOCUS       DACDXO010000016        44938 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E23605_2017
            SAMN12639989-rid9881463.denovo.016, whole genome shotgun sequence.
ACCESSION   DACDXO010000016 DACDXO010000000
VERSION     DACDXO010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639989
            Sequence Read Archive: SRR10023389
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 44938)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44938)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741914.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:42:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,972
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 33 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 17 of 73
            Pseudo Genes (multiple problems)  :: 10 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44938
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639989-rid9881463.denovo.016"
                     /isolate="E23605_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(38..1189)
                     /locus_tag="IWM35_001062"
     CDS             complement(38..1189)
                     /locus_tag="IWM35_001062"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HAP9918928.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
     gene            complement(1202..1639)
                     /locus_tag="IWM35_001063"
     CDS             complement(1202..1639)
                     /locus_tag="IWM35_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP9918929.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGCVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            complement(1654..3420)
                     /locus_tag="IWM35_001064"
     CDS             complement(1654..3420)
                     /locus_tag="IWM35_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HAP9918930.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEALATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            3684..4361
                     /locus_tag="IWM35_001065"
     CDS             3684..4361
                     /locus_tag="IWM35_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9918931.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            4364..5491
                     /locus_tag="IWM35_001066"
     CDS             4364..5491
                     /locus_tag="IWM35_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9918932.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLPLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLPDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(5691..6131)
                     /locus_tag="IWM35_001067"
     CDS             complement(5691..6131)
                     /locus_tag="IWM35_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP9918933.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAKTIEEVQMIFEHGHI"
     gene            complement(6142..8175)
                     /locus_tag="IWM35_001068"
     CDS             complement(6142..8175)
                     /locus_tag="IWM35_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAP9918934.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            complement(8177..9880)
                     /locus_tag="IWM35_001069"
     CDS             complement(8177..9880)
                     /locus_tag="IWM35_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9918935.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKKSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            complement(9974..10924)
                     /gene="manA"
                     /locus_tag="IWM35_001070"
     CDS             complement(9974..10924)
                     /gene="manA"
                     /locus_tag="IWM35_001070"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HAP9918936.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIATEPGK"
     gene            complement(10963..11988)
                     /locus_tag="IWM35_001071"
     CDS             complement(10963..11988)
                     /locus_tag="IWM35_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HAP9918937.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKAVLPK"
     gene            complement(12197..13066)
                     /locus_tag="IWM35_001072"
     CDS             complement(12197..13066)
                     /locus_tag="IWM35_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extradiol dioxygenase"
                     /protein_id="HAP9918938.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            complement(13525..15417)
                     /locus_tag="IWM35_001073"
     CDS             complement(13525..15417)
                     /locus_tag="IWM35_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9918939.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            complement(15414..17141)
                     /locus_tag="IWM35_001074"
     CDS             complement(15414..17141)
                     /locus_tag="IWM35_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9918940.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            complement(17157..17807)
                     /locus_tag="IWM35_001075"
     CDS             complement(17157..17807)
                     /locus_tag="IWM35_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP9918941.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            18011..18307
                     /locus_tag="IWM35_001076"
     CDS             18011..18307
                     /locus_tag="IWM35_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAP9918942.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            18479..19099
                     /locus_tag="IWM35_001077"
     CDS             18479..19099
                     /locus_tag="IWM35_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HAP9918943.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(19191..20357)
                     /locus_tag="IWM35_001078"
     CDS             complement(19191..20357)
                     /locus_tag="IWM35_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP9918944.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(20369..20722)
                     /locus_tag="IWM35_001079"
     CDS             complement(20369..20722)
                     /locus_tag="IWM35_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HAP9918945.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLETVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            20850..21872
                     /locus_tag="IWM35_001080"
     CDS             20850..21872
                     /locus_tag="IWM35_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HAP9918946.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            complement(22311..22745)
                     /locus_tag="IWM35_001081"
     CDS             complement(22311..22745)
                     /locus_tag="IWM35_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9918947.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLKKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(22855..24714)
                     /locus_tag="IWM35_001082"
     CDS             complement(22855..24714)
                     /locus_tag="IWM35_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAP9918948.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            complement(24832..25992)
                     /locus_tag="IWM35_001083"
     CDS             complement(24832..25992)
                     /locus_tag="IWM35_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-3-phosphoglycerate dehydrogenase"
                     /protein_id="HAP9918949.1"
                     /translation="MYHVHLIDAIPEQEIEKLQGEYFKIIDDLPAEGIVIRSTKVKDE
                     WLTPDLLAISRAGVGVNTINVEKASENGTIVMNTPGVNANAVKELVLCCLLLSSRPII
                     EASRMVQTLTGPNILEQAENKRSAYVGRELQGKTIGLLGLGAIGTKVALSCYSLGMDV
                     LGYSIRDAQLDYVRQADLETVLSTSDYIVVMLPLTEDTKGLIDQANIEKMKKDAVLIN
                     VGRSEIVDKYAVMQALEKQHLAKYLTDFPEEEFLENDRILMLPHLGGSTQEALADSGR
                     LAVEALKDYLLFGTVREAVNYPSARMFFQAPFRFTIFYQKKTNILAEIFSLLNENELA
                     IADISRNHKNEHVYILIDIDSTDFEQLNQVKQQLEKISGVRKVRLLKKPERP"
     gene            complement(26299..27723)
                     /gene="araA"
                     /locus_tag="IWM35_001084"
     CDS             complement(26299..27723)
                     /gene="araA"
                     /locus_tag="IWM35_001084"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HAP9918950.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            complement(27739..28437)
                     /locus_tag="IWM35_001085"
     CDS             complement(27739..28437)
                     /locus_tag="IWM35_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HAP9918951.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            complement(28394..30055)
                     /locus_tag="IWM35_001086"
     CDS             complement(28394..30055)
                     /locus_tag="IWM35_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HAP9918952.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKR
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            30273..31673
                     /locus_tag="IWM35_001087"
     CDS             30273..31673
                     /locus_tag="IWM35_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HAP9918953.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKVLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIIGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            complement(31925..33004)
                     /locus_tag="IWM35_001088"
     CDS             complement(31925..33004)
                     /locus_tag="IWM35_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP9918954.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(33090..34049)
                     /locus_tag="IWM35_001089"
     CDS             complement(33090..34049)
                     /locus_tag="IWM35_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HAP9918955.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSEKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            complement(34061..35584)
                     /locus_tag="IWM35_001090"
     CDS             complement(34061..35584)
                     /locus_tag="IWM35_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HAP9918956.1"
                     /translation="MDEEVKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHE
                     TADGDGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIE
                     PNSFGLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQ
                     KKPFGVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPR
                     LPSYPEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFD
                     RQIEEIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMED
                     TLLFGSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAK
                     GQVLKQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLL
                     DQGEHYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            complement(35594..36157)
                     /locus_tag="IWM35_001091"
     CDS             complement(35594..36157)
                     /locus_tag="IWM35_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HAP9918957.1"
                     /translation="MKIVETKFYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTIVLSISGY
                     VVLLIYSMFLLSWIYVISENKDITFLQIFEQSAWKVFFDFLRNILCCVLLLGFVLLIF
                     LFPPFIFIFAGGVWKLVSIILTIGNER"
     gene            complement(36163..37026)
                     /locus_tag="IWM35_001092"
     CDS             complement(36163..37026)
                     /locus_tag="IWM35_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP9918958.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            complement(37040..37936)
                     /locus_tag="IWM35_001093"
     CDS             complement(37040..37936)
                     /locus_tag="IWM35_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP9918959.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            complement(38111..39382)
                     /locus_tag="IWM35_001094"
     CDS             complement(38111..39382)
                     /locus_tag="IWM35_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP9918960.1"
                     /translation="MKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTLTFWNGF
                     TASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFIALNYPD
                     FAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMYMYWNND
                     LFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLANGGKLV
                     NDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIEINGPWL
                     NNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFLKYWNTT
                     EIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQISAINNDI
                     INPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            complement(39372..40889)
                     /locus_tag="IWM35_001095"
     CDS             complement(39372..40889)
                     /locus_tag="IWM35_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HAP9918961.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
     gene            41087..41977
                     /locus_tag="IWM35_001096"
     CDS             41087..41977
                     /locus_tag="IWM35_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HAP9918962.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            complement(42050..42874)
                     /gene="nadE"
                     /locus_tag="IWM35_001097"
     CDS             complement(42050..42874)
                     /gene="nadE"
                     /locus_tag="IWM35_001097"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HAP9918963.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(42886..44358)
                     /locus_tag="IWM35_001098"
     CDS             complement(42886..44358)
                     /locus_tag="IWM35_001098"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HAP9918964.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
BASE COUNT        12989 a         9387 c         7783 g        14779 t
ORIGIN      
        1 aaaaatttcg tcacattccc ctttttcgta tcctatttta agcgacttgc ttggcataac
       61 ggttcacgtt ttgcttgata ttcccgattg ttttcggatc attgattcct gtgacttctt
      121 gaatcaattg ttccaagttt tcatggatct tcatttcatg caattgacgg ctttgttcat
      181 cttcaggatc atagtaatta aaaataatcc ccattgttgc cgtcaaatgt gggcaagtca
      241 agttatgttc ttgtagttct ctcactggac gtgtaaatct ttcttgataa cctaacttac
      301 gtaatggtgt ccgagcgaca cgtgtgatcg catcagagat atttttgttt tggaagcgat
      361 gaatgatttt ttcaatatac gcatgatgct gctctgcatc aaacccccat ttggcaatca
      421 gcaaacttcc tgtttcctgc aaaactgatt ttaattgtgc cacaactaac gcatcttgca
      481 ttgcttcatc aatggtctgg taacctaaca aggcacctgt ataagcgaca gtcgcatgac
      541 cagtattgac actaaatagt ttgcgttcga tatatggttc tagatcatct acatataaaa
      601 ccccttttaa ttggattttt ttattttttc tttggctatc gtcaatgacc cattctttga
      661 aaggttccac ttggacgaat aaaggatctt catggtgttg taatgggacg attcgatcga
      721 ctgctgcatt tggaaagccg atatatttgt cagtaaatga tgtttccgga agatatttat
      781 acacttcttt ttctagaaat gctgaaccgc caatcatatt ttcacaggca atgatatcta
      841 aagatgttgt gcttccttcc ttttctcttt cttggatacc ttttgcaatc aactcagcaa
      901 taaaaggtaa gatattcggg ccaatggcgg tagtaactaa atctgcttca gcgatttctt
      961 ggattacttt ttctggattt gtttgattgt tgattccttt gacatttttt acagtgattt
     1021 tttcttttga ttcatctgct aattcgattt cgtattcttt tctttcattt aaagcatcaa
     1081 tgatcgtgtg attcacatct acgaaagtaa tctcaaatcc attagctgcc aaaacctctc
     1141 caataaatcc tcgaccgata tttcctgcac caaaatgtac tgctctcatt tttctctctc
     1201 cttaagcaat tgctagattt tccgttactt cttctttgct gagcgcgtct gctagctgga
     1261 caacattgtc catatcactg caataaagtg cgatttgctg tacaagttgc aaatgctcat
     1321 ctccgactcc tgcaataccg aacaaaacag tcgcaatctt ttcttcttct tcggtaccaa
     1381 aatctacacc atctggtact tgaacgacac agatgccgga ttttttcaca tatttttttg
     1441 cttcatctgt tccatgagga atcgcgataa aattccccat ataaacagat aacattttgt
     1501 ctctatctac catggcatcg atatattcct catcaacgca ccctgcttct acaagcatct
     1561 gaccgcaata gcggatcgct tcttctttgg ttttaaaaga tcgattgagc tcgatcatgt
     1621 cgaatggcaa actttgcatg ctgttttcct cccttaagct gtctgtttca aatattgtgc
     1681 aatcgattcg tatttttgcg gattgaccat gctgttgact gataaatgaa tggattgagg
     1741 tgcagccatc tgtacttttt ccgctaaact ttctttggct acgatcagtg aatcaggttc
     1801 atcttttaaa tcttggatat tgacttttct taacgggata gttaaatggt tcttgtctaa
     1861 tatatctttg agcacggtca tccccattgt tgctgatccg gaacgatcat cgtggacaag
     1921 tagaatctct ttgatttgtt tgatgtttgc tgaaagaaac tcttctgctg gctcattctc
     1981 aatttcagaa actgatgttt tctgcttgct gcttaattct tgaatgattt catcatatcg
     2041 aggtgaattc aaaaagtttt ctactgagac aaaacgcgcg ctcggtgctt tttgttttgc
     2101 tctttcttgt agttcgacct gagtaacgat caatgtattc gagtcatctg tcagttggct
     2161 gattgcttga ttggtcaccg tttgaggcaa gtctgcttcc tgtacttttt ttctcaagat
     2221 cgaagcgccc atcgcacttg atcccattcc tgcatcacaa gcaaaaatga ttttttgtac
     2281 gccagagaat gtagctcctt gaggaatacc ttgggcacct ttggaagctt gcttagctgc
     2341 ctttgtctct tgaacagatt tttcgaagtc ttcatttgta tctttcgcat ccgctttcaa
     2401 aattactgca gaaataccaa acgaaacagc tgcggcaaca atcacgccca aaataacagg
     2461 aaggtaagaa ttgaacggtg tcatcgctag aatcgcaatg atcgaccctg gagaagctgg
     2521 cgctcttagt ccagcatcca tcaattggaa aatgaaactt cctgacattc ctccgcctat
     2581 aactgataag aataataacg gtttcatcat aacgtaagga aaatagattt catggatccc
     2641 gcctaaaaag tgaatgatca tggctcctgg tgctgaagat tttgctgctc ctttgccaaa
     2701 caaagtaaaa gcaagtaata cgccaagtcc tggaccagga tttgcttcaa gcaagaatag
     2761 aatagatttt ccagcctctg ctacttgttc agttcccaat ggtgtcaaaa tcccatgatt
     2821 gattgcatta ttcaaaaata agatttttgc tggttcaata aaaatacttg tcaatggaat
     2881 caaatgggca gataaaatga tctctacacc ttttgccatt gcttgagtca atgcatcgac
     2941 aactggacca atcgcagaaa aacctaataa cgctaatcca aaaccgataa gacctgctga
     3001 aaaattattg actagcattt caaaaccgga tttgatccgt ttttggaata gctgatcaaa
     3061 tttcttgatg gcatatccac caagaggtcc catgatcatc gcgcctagca tcattggaat
     3121 atctgttccg acaatcacac ccatcgttgc aattgcccca acaactgatc cgcggtcacc
     3181 agcaattact tttccgccag tataaccgat caataaagga atcagataag ttagcattgg
     3241 gcttaccatg gtagccaaag cctcatttgg taaataacca tctgggataa atagagccgt
     3301 taatacaccc caggcaatca gtgctccaat atttggcatg accatgcttg ataaagtact
     3361 gcctaatttt tgtaccctcg cttttagcga aatgttgttt ttgggaatcg tccgttccat
     3421 tttgtctctc cctctctcct tttgatactc taattgtatc cgcttactaa aaagagaaat
     3481 agtcgtactt tggcacgaaa ctttgtgcca aaggtttgag tatgtcagca aaataaaaag
     3541 atttcggtat cagaaaactt caattttaaa taaaactgtt attttaattt ttttataata
     3601 tgcaaacaat aacttttgtt agcatttaat ttagatgttt tccattctat tatgttatta
     3661 aattttatat tggaggccta tctatgacat tactgatcaa aaacatgaac atgaaacgtg
     3721 gtaaaaaacg tgtgattcga caattgaatc ttttagtaga atcaggagaa gtagctgctt
     3781 tgattgcccc gaacggattt ggcaaaacaa ctttgctgaa tggcattgcc caactgctgc
     3841 caacaactta tgatcaattg tcccttcaca acatctctcc tactgcttat acagattatc
     3901 gccggtcttt cttttttcta gaatcaacga atcagttatc cgactatcta tcggctgttg
     3961 attatttgac tttgatcaaa tactattggc agagcaaagt agaatgccaa actgttttcc
     4021 aacgattgaa gatgactgag taccttactt tgccgattgg aaaaatgtct ctaggaatga
     4081 aacaacatgt cttgcttgct gcttacttga tcagtgattc atctgtcatg ttattcgatg
     4141 aaccgttgaa cggattagac ccaggaagta ttgatatctt gaatagcctc atcagacaat
     4201 ggcagaaaga ggggaagacg attcttcttt cttcccacgc tatcgcgaat atccaatcga
     4261 tttgttctaa agcatgtttt ctaaaaaatg gagaaatcgt cacagaatcc tcaaatatgg
     4321 aagagatttt agctatttat cgtaaattat atgaggtgta actatgtctt tacttacatt
     4381 tgaacgcaat ctcttactaa aaaacaaagt aaacttttta tttcctctat tgttggtcgt
     4441 gctctttgca ttcccacttt tttttgatca taaacttgct tatacagaat tcgatgaact
     4501 gaatcataac tatgaagaga tgcaacgttt gattgaaacg ttgaaagaag atgagaatga
     4561 gaaagaattt gtggaaagtt tagagaaaag caacaaactg atcgaagcaa tcttacatgc
     4621 aaaaaacaca gggaatgtcc agcaaatggt cgaagcaact tatcattatg aaaaagatat
     4681 actggatcga ctgatttctg gtcaaagaca aggaattcca atcattgaac aacaaaaacg
     4741 tgtcgagttg ctccgttata tgaaagaaca tcagatccaa agatattcta tttttgactt
     4801 gccagcgcat ctccctttag caaattacta cgaaaatatt ttttcaggaa tgatttctag
     4861 ttttttgatt ttatgtataa ccgccttgtt tctttcttcc atcatctctt atgaaaaaag
     4921 aaagcaggta atctctctag tcaatctatt accggatagt atggtaaaga aacatagcat
     4981 ccgttttacc atttattatg gtgcagctat gctaagtctc gtgatgccat ttttgatcgt
     5041 ctctatacta gtaataatca aaaatggttt gggagacttc cgttatcctg taggaacgat
     5101 cattggtcag gagatacgaa tccttccgat gtatgagtat cttttccaga gctttctgtt
     5161 cctgttgttg tgggtgctct ttttaagtac aatcagtttt cttctatctg ctttatttga
     5221 acattctcta gtcaatcttt taggtacact tctttgtctt ttcttagcag agtatcgctt
     5281 gttttcttcg attggatgga tagaatcaat cagtcattac ttgccaactt cttatgttga
     5341 ttttcaaaat gtgatcatag gaggagatat tttctcgcca ctagcttcag aacaggttac
     5401 tttcatgaat ggtatcttga cacttggtat atggagtatc gttttattat tcataggtat
     5461 gggcactatc tatataaaaa agagctatta aaacacaaaa ataatagggg cggtgacaaa
     5521 aaccttgtca ccgtccccta tttcaaataa acggttttct agaataaata gttttcaaaa
     5581 gcttgcatcc gcagtaataa gaacctccca acaaaaatag ctcctcttat ttttatcttg
     5641 gttgaagtta ttacttgatg caaggcagct ttttttactg tgaacattgg ttatatatgt
     5701 ccatgttcaa aaatcatttg gacttcttca atggttttcg catcactgag cgccattact
     5761 tggtcgatgt ctgaacagaa aaaggctaaa tcttggagca ccgctaactg atgttctttt
     5821 tttaaagcca ctacaaataa gagtgtcgcg atttttggtt cctcttttgt tccaaagtcc
     5881 acaccatcag gcacttggac taaatagatt ccttcttcta ctacttcttc atctttgtca
     5941 gtcccatgtg gcaaggctac gaaattcccg atatagacac tcgctttttc ttgtctttcg
     6001 atcattgatt ggatatatgc cggtttgatt cccccttgtt gggtcatcca ctggccgatc
     6061 tcggtcaacg cttctttcca attttgatag gattgatcga gcaatatttt ttctgctggt
     6121 agctgtatca tcttttcacc ttcatttctt tatttcttca atcaggattt tcgctaacaa
     6181 ctgataaacg atttctttat ttcctgagcg gaagatctcg gtgttcaagt cattcatgat
     6241 gattgctcca ctgatctttc ctaaaagaat cccgtctttt tcttggattg gcgaaggcgc
     6301 cagcataact aacatacggg acaaatcgac tggctgtcta tccatccctt caatggtcaa
     6361 cggatgtttc aaattgaaaa tacaaaagat cggtttagaa atcaatgagc tggatgtatg
     6421 gaataacccc atatttgtat gaggaatacc gataggtgct tgttcatatc gcttaagcag
     6481 ccggcgatga accacctctt catccgttac tagtccttca ggaaaataag gaaataagct
     6541 gcgaatcgtt tcggaaatcg tctcaggatt atccagttct tggataaaaa aacgaccgag
     6601 taattgattg atttgatcca tgaaatgcat tgtttcctca taagtatctt ctatcatcca
     6661 ctcgttattg ctgttatacg taattttttg cctttttttc ttctcaatat gcgaaaaagc
     6721 ttcttttagc tgcttgactt catcttctag cagtagcggg gagactaaaa tatattttcc
     6781 accgtatcct ggtaaaatag atgtagaaat aacaacatca tattcttctt gtaaatcgat
     6841 tttttttaaa tcagcaatcc gatagaaatg aatttgttgg atgaagggaa aatacctttt
     6901 caatcgtagt tccaacatgc tagtagaagc taatccactt ggcgaaaatc ctgcaatact
     6961 gatattgctt tctttcggac ttcgttccaa tgaattcgca aaatgaagga ccatataagc
     7021 gatctcttct tcggataatt ttttttcttg aaaaatccgc tctactgctt tacggattgc
     7081 attcgcaact tcctcgtatt gaaccatgat tttttctaaa atcggattgg tcagtgtctc
     7141 ttcttgtaaa acggctcttg aaaacacacc agataaatga gtcaacagca ttttgtacaa
     7201 agtttcatct tcataaaaat ggacattcgt ctcttcactt acttcttgaa tcagttgttt
     7261 tactaaataa gcaagctgtg tatcgaacgt ctcttcaaag aaattctgat caaatgagtt
     7321 cgtgaagtca tttaataatc ctgcaaaaaa gacgatctca tttactggat aaagttgttt
     7381 tgtctcagaa gcgatccgtg aaaacaatcg tttagctgtc tgtagtaatt ctttgtttgc
     7441 cagactatta tatgtctcat ttgtcagtaa atgattttta gtcacacgct ccatcgtcaa
     7501 tgtcagcatc aaaaccaaat gttcaagctt tctatcactt agattggtct ttttttggat
     7561 caattcttcc ccgattactt tgcgtgcaag agatagatgt tgcggatcaa cgaatcgaaa
     7621 aaaataattc agtgatgcat cgaaagaaat actatgaaaa agttcatatt cattgatttc
     7681 taattctaat acattcgctg tcagaagccg gcggtatttt tccggtcctt cgatctcgta
     7741 tccctggttc cgaatgattt ccaaaggcag ttggcgaata ctttcttcca actgtttgat
     7801 atctgcataa aaagtggtat tactgatgcg atatgattcc atgaaatgag caagactcaa
     7861 cggctctgtc gtcagtaaca actcggctaa aatcgcgtgt tgtcgtttag cagtggataa
     7921 gtcattactt ttcccatctg tattttcttt taattgctct aatacttctt ctgatgcctc
     7981 aatgacaaat gagcctctag ctgctttttt caaagaagca tttaccgatt tcaatgagac
     8041 ttcaagatta tctaattcgc ggtaaatagt ccgtttactg accttcaata aattcatcat
     8101 tttttggata gatacaggtg acgggctact gagaaattct gctaataatt gtttttctct
     8161 gggcgataaa tacatcttat atttctccct tcacgagttt ttcgtattca ttcgtagcta
     8221 cccaatgttc aactagaact agccgaggga tcttttcttc tagctgttca gttacagcta
     8281 gttctctttg tgcgatataa atcgtttctt cttggatttt gattttttct attgattctt
     8341 ttgagataac aaactgtttt ttcgcttttt ttgctaattg acttaaaatc gtcatgccca
     8401 tagtttgtga gccgcgtctg ttgtttttat acaaaaatac aatattagca ggcgactttt
     8461 tttgttctgt gatatctgct atctcttttt tttcttcccg ctttggaaat tggatcgctt
     8521 cttttattcg tgacagcaag atatctgttt ccaaaaagtg atccagtaca tagatttgac
     8581 tttgaggtac tttttctctt gcaagacccg ctagttcatg ttgcacgata accagctgat
     8641 ttacttggtt attcaattca tagatagagc tgtatccgac agaaagatcg attttttctg
     8701 ctgctaattt ctttttcagc agggcggctc ccatagcact tgatcccata cctgagtcgc
     8761 aagcaaaaat gacggaatga atgatagatg gttccaagat ttctcccctt tctttgaccg
     8821 ttttgttctc tttttcttta tttggtaatt tcaatagtat cgctgcacaa atgaaggtca
     8881 caaatgtact gattgctatc cctatactta ccccagctag ttgtggataa ggcgcattgg
     8941 caaaaatcac gagtagtgac ccaggcgata caggtgcagc taatcccgta tctaaccatt
     9001 gaaatatcaa cgtgccgctt gccccaccag ccattacagc taaaaataga tacggtcgca
     9061 gcaagacaaa tggaaaatag atttcgtgaa tcccgccaat cgcatggatc atcaatgctc
     9121 cactcgcatt tactttttcc tgtttttctg caaaacaaag gatagaaagt aagatcccta
     9181 aaccaggtcc aggatttgcc tccatcaaaa ataaggaaga gtacccgact gtttgtgaga
     9241 attcgattcc taaaggtgtc aatacaccat gattgattgc attattgaaa aatagtactt
     9301 ttagaggttc taagatcaga ctgacaagtg gtaacagatt cattgtgact agccaagaca
     9361 caacggaggc gagcaacaag tgaacactat ttaaaactgg ttcaataaaa acgattccaa
     9421 ttatacaaat aacgctacca gtcagtccta ctagtaggtt gctgacaagc atttcatatc
     9481 cagccttgaa tttcacgtgg aatctagaaa caaattgttc atatacgatg ccggcaagcg
     9541 gcccgataat catcgctcct acgatttgtg gagcattgga acgagcgatc aaccctaaag
     9601 aagcaattgc accggcgact gccccttttt gtccccctac cgatttcccg cctgaataac
     9661 tgatcaagat gggtaaaagc agctggatca tccaatggca tacctcttct aatgtggtgt
     9721 gcaaatcgcc agacacacct tttgttgcta atgacaagat tccccaagcg ataaacagac
     9781 ttagatttgg catgatcatt tcatttaagt gatgtccagc ttttctcagt tgtttcatag
     9841 acacgcgact gctgttgttt gctttccatt tgtattccat ctttttcacc atacttctat
     9901 ttattgatgg acggatttta tgtagaaaaa gcagcaaacg aaaaaccgtc tgctgctttc
     9961 agctatctgg gaactatttg cctggttcag tcgcaatgat tgctagttca ccttggaccg
    10021 cccactcaga aacgccattt ggaataatac agcttgttcc tttttctaat ggatatgttt
    10081 ttccgtcaac gacaaggtct cctgctcctt cgattaccgt catcaatgta taaggggctt
    10141 gttttttgaa agatgtgatt cctttaatat cccactcata tacattgaaa aactcagttt
    10201 ctaaataagt gacgatcgat gaattccctt ttcggacttc tttgatttgt aattctggtg
    10261 ttttcgcagg gacagttgtt acatcgatcg attgttggat atgcagctct cttgtttgtc
    10321 cttgcgcatc ttttctgtcg tagtcataca cgcggtaagt cgtgtcagaa ctttgctgtg
    10381 tttctaaaat catgattcct ttaccaatag catggatcgt tccgcttggt acataaaaga
    10441 aatcaccttt tttaactggt actttcttca aaaggtcatc ccagcgccca tctttgatca
    10501 tctcagctag ttcttctctt gtctttgcat gatgaccata gatgatttct gcaccaggtt
    10561 cagcatcgat gatataccag cattcagttt ttcctaattc accttcatgt ttcatcccat
    10621 aggcatcatc tggatgtact tgtacagata gatcatcttc tgcgtctaag atcttgatca
    10681 ataatggaaa cactggttct gaaggatggc caaacaattc cttgtgttct gaccatagtt
    10741 catctaatct tttccctttg aattcgccat tttctactac actgacacca tgaggatgcg
    10801 cactgatcgc ccaatcttct ccgattttat cgttagggat atcaaaacca aagactgaac
    10861 gtaaacgact tcctccccaa attttttctt gaaaaactgg ttttaaaaac ataggttcct
    10921 gcataattca aactcctctc atttttcata gctattatag cattatttcg gtaataccgc
    10981 tttctctttc aaaaagtttt caacaatttc atctcgacat atgagatact ccgcacgatg
    11041 atcgacagga tgtacgcgat ttgacaaaaa tatcaacgct tcttgtgttt ttatatcaat
    11101 caagaaaaac gtacctgtat aaccagtatg aaaaagaatc gcatgaccgt cttctacatc
    11161 cttttttaag tcccatccta aagaacgata gccatttttt ttcggtgttt gatcttgcag
    11221 taaagaatca atcgtctctt cttctaaaaa acgaccatta cggatttttc ccttgttcaa
    11281 atacatttgg acaaaagtaa tgctgtcttt caaatcaata aagagacccg cgttacctgc
    11341 atgttcagcc aaaactcttg ctttcggatc atgagtttgt ccacgcaaaa cctcacccga
    11401 aggcagttgt tctgtgggaa cgattttttt ggaaggaaaa ggaagaaata agctgttcat
    11461 cattcctaat ggttccaata cttgttcttg aaagatttcc actgcagttt cttggtaaat
    11521 ttcctctagc atcaacccaa gaagaatcgt accggcatct gtatatttta cttcttttcc
    11581 tagttgctca cctggttcta gcttcaaata agcggcgatc aattcttcct gagatagctt
    11641 atctctgttt ggtatccatg ttttgatatc agaagtatgt gtcagtaaat gacgcaaagt
    11701 gattgtctga ttttcaaatt caggaagata tttatgaagc ggttgatcga gttctatctt
    11761 tccatcttct aatagacgta aaactaatga agtggttgca acgactttcg tcagtgaggc
    11821 tgcatcaaac aacagatctt ctgtcatcag ctgcttttca ggaacgactt gggcaaagcc
    11881 ggctgtaaat gtttctactt gattttcttt tatgaaggcg actgctacac ctggataaaa
    11941 tccttcttcc atctttttca aaataagctg tttcgtgtgt ggatacatct ttttctcctg
    12001 cttttctatc agattcttac agtccaagct taaagcttaa tctatgagga atcaaccttt
    12061 tttacacaaa aaaactgcgc gaaaaaccgc gcagtttccc ccggtatact attttctctt
    12121 tctatctcta gaataagcga tagtatgacc gttcataaaa tgacgagaca tgcgacaatc
    12181 gttaagaaag aaaaagttaa gcgtcgcatc gaatgtcagt tttattcttt tcttttggct
    12241 cactgacgaa ccaccattcg attcctatag catcatagat tgtgagtact gtgtgctttt
    12301 tgtctaagaa gaaatcaact gatagttgct gcaagtgagc aatcagtcct tccatgcttt
    12361 cagtatcgat catgaatttc agaaaatcaa gtccaatcac ttcatctgta ggcaaatcaa
    12421 ttgcgcctcc gctagcttct gtcaaaccaa catggaaatc tccttcattc aatacatgga
    12481 tgccctcttc ttcattatgt accttcaaac caagtacatg attaagaaat tggcgttctt
    12541 ttgttaagtt agtaacattt aaatggattt tttcaaaata agaacctgtt gatagtttac
    12601 tgaatttttc ttcagatttt cttaataatg cttcttgatc cattgtttct ggaatttctc
    12661 tttggtcgtt cttattctct tcatggtaaa tttccaattc attctcttct gggtctacga
    12721 gcaaaatccc catacgtcca tgatcttcta aagcttcttt gattggataa ttcattttac
    12781 ggatacgaca aaggatatct gccatttctt ctgtgctagg gataatcaat gaaaaacggt
    12841 tcagcttttt gatcaccccc atgtgatcat tcgctctagg actttcttcc agcagcaaca
    12901 tttcacttcc agcttcttct gttcctaaaa tggctaattc gttttcttct cttttcaaat
    12961 caaagccaac aacgttcttg taaaaatcaa tcattttatc tcggtctttc acccgaatgg
    13021 ctacactttt taaataggtg gcagccccca attgaaaatc tttcatattt cttgctcccc
    13081 tctacgtcaa gctttatttt aacgatggat tcttcttctt tcaagcaata actttgattt
    13141 tgttaaaaaa aatgtgagaa aattcgcatt ttgcttgttt tttgtaaatt aatgagtaaa
    13201 aactcttata aaaagaggtt gtgaaaaagc tgttctgcat caagtaataa gaacagccaa
    13261 acaaaaatag ctcctcctat ttttcgtttg gttgggctta ttaccgcaga tgcaagcttt
    13321 tgaacaccgt ttaaggaaga ggttgtaaaa tcagcggttt gacctgagta ttagagatac
    13381 aaaacggaaa aatagcttct catattttgc cgttttttga gctaataccg aaggacagct
    13441 gattgaacac cgtttattcg gaaaaaggga ctgtgacacg acttttgtca cagcccctaa
    13501 aaaaaataat aaatttgtac tattctatga agcttcttct tcagaaaact gactgttata
    13561 aagatctgca tagaatccgt ctttttccat caatgtatcg tggtttcctg tttctacgat
    13621 cgaaccatga ttcatcacaa taatgttgtc tgcatcgcgt attgtagaca agcggtgagc
    13681 aacaacgaaa cttgtccggt tttccaatag gcggttcatc gctttttgaa tcagtatttc
    13741 tgttcgtgta tctacactag atgttgcttc atcaaggatc aggacatctg gatttgctaa
    13801 gaatgcccgc gcaattgtga ttagctgacg ctgcccttgc gaaatattgc ttgcatcctc
    13861 gtttaataca gtgttatatc cttctggcaa tttacgaaca aaatcgtcta cgtgagcagc
    13921 tttagcagcc tcaatgattt gttcgtctgt tgcatcgtcg ttcccatatt tgatattgtc
    13981 atagatactt cctgtaaaca gccaagtatc ttgcagtacc attgaaaact gtgcgcgcaa
    14041 ttcatcacgt gaaagatcac gggtatctac accatcgtaa cgaatactac ctccagaaac
    14101 atcgtagaac cgttcaagaa ggttgatcag tgtcgtcttc cctgcaccag tcggacctac
    14161 aatagcgacc atctcaccag gtttgacatc caagttaaaa tcagtcatta gcaagttatc
    14221 tggtgtgtag ccgaattgaa cattttcaaa acgaactttg tatggtgtat ctttttcttc
    14281 tggaaggcca gatttttcat tggacatctc ttcttcatcc aacacttcaa atacccgttc
    14341 ggctgaagca actgttgatt ggatagtgtt catcaaactt gcaagttgag tgattggttg
    14401 agagaactgt gttgtatatt gcaagaacgc ttgaacatcc cctaatgtca tgttcccatt
    14461 tgcaactttt actccgccta gtacagcgac gaacacatag cctagatttt taacgaaatt
    14521 catcaacggc atgatgattg ccgaaataaa ttgagctttc caaccagcgg catacagatt
    14581 ctgattttct ttttcaaata cttcctggtc tttttcagta tgattgaatg ttttaacaat
    14641 ggtatgacca ccatatgttt cttctacttg gttattcaat aaacctaaac ttttttgctg
    14701 atctgcgaaa aacacttgtg atttcggcgc tacgatcatc acgatgatca agcttaaagg
    14761 gatcatcaat aacgcgacta acgtcaattg ccagctgatt gaaagcatca tcacgatgac
    14821 cccaatcaat gtgactgcac tagttacaaa ttgagtcaaa ttttgctgca gcgtgcttgc
    14881 gatgttatcc atatcattga ttgctcgaga catgatatcc ccatttgtat gtgtatcata
    14941 ataggaaatc ggtacacgat tcattttggc ttccaaatca tgacgtaatt cataaactgt
    15001 tcgttgggaa acacgtgtca taataaattg ctgtaaaaag ttgaatacag cagaaatcaa
    15061 atacatcacg attactgtcg caatgatctg cgcgatttta tcaaagtcta tcggaaattt
    15121 tgttacttgt tggccttgtt gcatctgttg agcgccagcc attacacctt tgaatatttc
    15181 tgtggttgct ttccccaaga cctttggtgt ttggatttgg aagatcacgg ctgctattgc
    15241 tagtacaaat acagcgatga tcgcaaccat acgtttagac atatagccaa acaatcggcg
    15301 taccgttccc cagaaatttt taggcttttc accttttaca ccgagatttc tgccaggtcc
    15361 accatgtcct ggaccgcctg ctggtccatg agagggacgt gtgtttgttt tactcatgct
    15421 aagtcctcct ctcttagttg tgaatgaacg atttcttgat aagtttcgtt tgttgctaat
    15481 agttcttcat gtgttccttt gccaaccagt tttccttcat ctagcacaag gatcatatct
    15541 gcatccatta ctgtacttac tcgttgagca actaaaacgg tgatactttc tttcatattc
    15601 gttttcaatg cttggcgaag attggcatcc gttttgaaat ccaacgctga gaaggaatca
    15661 tcaaacacat agatatctgc tgttttgact aatgcccgtg caattgccag acgctgtctt
    15721 tgtccaccag agaagtttcc tcctccttgt tccacatggc tatctaagcc atcttccatc
    15781 tctgaaacaa aatctttagc ttgtgcgatt tctaatgctt tccaaatcat ttcatctgtt
    15841 gcatccggct taccatactg catattgtct cggatcgttc cagagaacag aactgctttt
    15901 tgaggaacaa agcccattaa ttctcgcaaa ttatattgag tcatgtcact aatgtccgtt
    15961 ccatttattt ggatcgaacc agattcgatg tcatataatc gagggatcaa attgactaag
    16021 gtcgttttac ctgaaccagt cccccctatg attgcgacga tctcgcccga cttggcttgg
    16081 aaatcaatat cttccaatgc aagtttttct gctccatgat atcgataatt gacatgattg
    16141 aaggccaatg ttgcgttttt tccagcaaaa gaaactgttt taggattttc agggtcatgg
    16201 ataccgattt tttcatccaa cacttcatta atacggtcag ctgaagcttg tgcacgaggc
    16261 accatgataa aaatagcgga aagcatcata aagctgatca agatctgcat cgcataagtc
    16321 ataaatgcaa tcagattacc gacttccaaa gtcatatctg ctatgtagtg tccaccaaac
    16381 caagtgattg ctacgtttgt accgctcatg atcaatgtca tcattggcat catcaatgcg
    16441 acgatcgtat taactttgat tgccgtattt gtataatctt tattcgctaa atcaaaacga
    16501 ttttcttcaa agcgtgtctt atcgaatgct cgaatcactc tgacccctgt caatccttct
    16561 cgaaatacca ggtttaaacg gtcagttttc ttttgcatac ttttgaataa tggaacagca
    16621 aagtacatga ttccgccgat caaaataatc attactggga tgacaactaa gaagattttt
    16681 gtcaactgat gatctttttg ataagcaagt atgctcgcgc caatcaacgt gattggtgca
    16741 ttgatcatca tacgtaaaaa catttgtgta accatttgga tttgagtcac atcattggtc
    16801 gttcttgtga tcaaagaggc cgttccatat ttatcaaacg catcttttgt cagactctct
    16861 gattttttat agacatccgt acgcaattgc ttacccagtt tctgtgattc acgagtagca
    16921 aaaaatgtat ttgcgatagc agctaagata ctgatcaaag aaaagccgat catcacaaaa
    16981 cctgtatgcc aaatataatc gacatctcct tgcgctaccc ctttatcaat aatattggag
    17041 gtaagtgtcg gcaaatataa atctgccagt acttggatca ccatgaaaac tgcagctgca
    17101 attgctgaag tcaaggagat ccgttttacg atttttagca tctagttccc ttctttctat
    17161 ttatatactc cgtttttcag ccaagaaagt ttcaaattga attcactgat tgcttgctct
    17221 actgagtaat cttcagatgt tgaaaataag cgatacgaat gcgcaattgt tgtaaataca
    17281 gtattcatca ctaattggat cagtaattcc aattcatgat cattttttac tttcagcttc
    17341 gtccgatcga tcaattcaag caactctttt gcatcttgtt tgtgttcttt atgtggatcg
    17401 tgaggcgttt tttggcgact cgtatgagga gcgacttttc ttgaggcacg gtaatccatg
    17461 ttcataaaca agttacgata aaaagaagca ttctcgcctt ccaaaatctc ataaatcatt
    17521 ttagaaaaat aggattcgaa tcctttgaat agatcaccat cggattcttt cattagttgt
    17581 aacatatctt tagagctgtc acgtctcatt gtctcaaagt aataaaaata aagatcctct
    17641 ttatcttcaa aatactgata gaaactgcct ctagggatat ccgctaattt gacgatatta
    17701 gcaatcgaag cttcttttaa gggtactcta gaaaactcga ttctcgcagc ctcgattagt
    17761 cttttttgtt tttctttagg caaatggaaa aatgtctgtg tcggcatatg gatcctcctt
    17821 tctttcaaga tgacacattg tcatatgacg ctgtgtcatt ataacacctc ctacctgttt
    17881 tgcaagacat ttgcatagtt tttttcatta tcatccgata cattaaaaaa gtgataagag
    17941 gaatccttcc atttgaatta atgagagttg cccattaaaa tagccacata ctaaacaaag
    18001 gatgagataa atggcccaat caattagtgt ccaagacttc aataaattgc ctctcgatgc
    18061 tgacagcatc gttttagata caagaaatac cttagattac acaaaaaacc acttaagcca
    18121 ctcgctttct atccccgctg ctatcttgcc aagacaatta aaaaatctag atccaaagaa
    18181 actttattat attctaagct atacaggcag gcgatcagaa gcattagccg atcaacttac
    18241 tgctaatggt tttcgtgctg tttatatcat tggtgggatg gaagctcttc agtcgagtgt
    18301 tgcttgaata ttgcttgtta gattcaaaag aggctgtgac aaaaaatcgt gtcacagcct
    18361 cttttatacc ctgtcttttt ttagttattt attcttcaac cttatcaata tgacttgata
    18421 gccttttctt tttctcatag ttataacaat catagcaata aaaggagaag gtaagcatat
    18481 ggcatctgtt ttagtagtca aaggtcatcc cctaactgca gaagaatcgc gcacagttaa
    18541 ggcattgacg tcttttttaa caagttataa agaaaatcat ccagatgatg aagtgactgt
    18601 tttggaactt tatcgcgatg atattccgga aatcgatgaa gaattattat caggttggga
    18661 agcattacgt gctggtgcag aattcacttc cttatcagaa agtcaacaac aaaaaattgc
    18721 tcgtttcaat gaattgactg atcagttcct tgctgctgat aaaatcgtta tcgcaaacgc
    18781 cttatggaat ctaaatatcc caacaaaatt aaaagcttgg ttcgataccg tcaatgtagc
    18841 tggaaaaacg ttccgctata cagaaaacgg gccagaagga ttagtgacag gtaaaaaagc
    18901 actgcacatc caatctaatg gcggtgtata caacggacaa gactttgcct ctcaatatgt
    18961 aaaaggtatt ttaaactttg taggcatcga tcaagtagat cagctattca tcgaaggaat
    19021 cgattatgac cctgaccgtg cagatgagtt gatgcaaaat gcattagata aagctgctgc
    19081 tttaggtaaa tcattttaaa aattgagata aatgcataca aaagagacag aggaaaaaat
    19141 cttcaaagca gcatttgtac cttggatatt ttcccatctg tctctttttg ttattctttc
    19201 tgctttttca acggattttc ttcataaaga gaagagattg ttgtgtacca ttcaaaaaca
    19261 tgagtaatca ctactttagc tgctgcataa acaggaatcc ccaagatcac accgatgatt
    19321 ccgaacaatt tcccagaagt cagcagaact aataaaatcg ttactggatg gattgctaac
    19381 tgattcccta gtactaatgg agagatcaag cgtccttcta ttgtctgctc tactgcaaaa
    19441 acgatcagta ctttgatgat catgaatgga cctgctacaa tggctaaaaa gatagccgga
    19501 atcatcgcta aaaatgatcc taagtaaggg atcaggttca aaaaacccgc aataatcccc
    19561 aacgtgacag catactctaa accaatgacc gcaaaaccaa tcataaacat gattgccaca
    19621 gcaaaagcca cagttagttg tcctcttata taagaagaaa cttgactatt catttcactc
    19681 agcacattca acgtcggttt ttgcatgcgt gtcggtaaaa atttgacaaa ataaggtgct
    19741 aattgtttac cgtctttcaa tagataaaat aagataaacg gcatcgtcag cagagccact
    19801 aaaatagtcg ctaccgcgcc gacaaaactt ccaagactct gaactgtact cttagatata
    19861 tcttggatca tatcgcctgc cgaattcatg aacttatcac ctgctgtttc caactgttcg
    19921 cgaaattggt tgaacagcgg gtcccgcaaa atctcggtta atttattatc aatcgtatct
    19981 acatattgag ggaagttact gatgaagctc actgtttgtt cctgaatctt agggatgatc
    20041 actacgctcc cccatgcgat tagtgcaaca accaaaacaa acagcccaat gatactgtaa
    20101 agccttggta cttttcgctt ttcaaagtaa tcaactaccg gattcatcaa gtaatacaag
    20161 attcctgcta aaatgatagg cagacctacg attgctaaaa attgccagat tggtgcaaac
    20221 aaatatgaaa cttttgtaaa taaaaagacg atcagcaata ttaacaatac aacaagtaag
    20281 gctgtgacta cttgattatt taaaaaccag cgccaaaacc acgataccgg tctgttcttc
    20341 ttattttttc cttccataat tcctcctgtt agttataaaa aatttggctt cccaaaccaa
    20401 tagcaggcag tctatgcgga tgaagataga ttccatttgc aatttgtcct tcttctggac
    20461 aagcatcaaa aatcaatgat acgtgagcta ctgtctctaa attcccattg gccatcggac
    20521 cgacataatc gatcgtatat acctgatcat caaaagcaat tgttccgcct gctttcaaag
    20581 aaaaaggttc gtcttttgat acttcttgaa tcaccgaata ttttcgtaag gcagctgttg
    20641 ctgttttatc gaacaaaatc agcatgggtt cttccttgtt tattgcttct gtcccaattt
    20701 ctgtaatctt tgcctgaatc attgcactct ctcctttaat tgctttcaaa aacagtataa
    20761 cacattctca ttctttagcc gtgtggaata attatgtgat agaataaaaa cgaaaactat
    20821 ttcattacaa ttaggaggaa tcagctcata tgatcgaaaa gataatttta ggaacctata
    20881 cgcgtcgcga aagtaaagga atctatacga tcgaccttga tacagaaaaa gaagaattag
    20941 ccaacctttc attagctgtt gaagaaaata gccctaccta tgtagcaaaa agtaaagctg
    21001 gcaatcttta cacagttact agtgtggatg gattaggtgg tgccggtgcg tacgataagg
    21061 attttcattt tttgaatgcg gtaacagagt ctggcgctcc tttgtgttat gtagcggttg
    21121 atgaagcacg tcaattacta tacggggcga actatcataa aggtgaagta aatgtctacc
    21181 atatattagg agatggcgaa ctgaaagcag cagattcaat attccatcaa gaagcgacag
    21241 gtcctcataa aaaccaagat catgcgcatg tccactatac ggatctgact cctgaccaac
    21301 gattagtcgt atgtgatctt ggtacagatc gtgtgtatac ttatgatgtg tccgaaaacg
    21361 gcaaattgaa tcttgtaact accttcactg cagagccagg aacaggccct cgccatcttg
    21421 tctttcatcc aaatggacaa ttcgcttatt tatttggtga attagacagc acagtccgcg
    21481 tgctttctta taataaagaa acaggtgctt ttgaagaatt acagaaaata tcgaccttgc
    21541 cagaagaatt cgacggtgaa aatggagggg cagctatccg catttcaaat gatggcaagt
    21601 tcctttatgc ttctaaccga ggacataact cgattgctgt attcgcagtt tttgaagatg
    21661 gttcccacat tcaaaacaag caaatcatct caacagaagg ggattttccc cgcgatttcg
    21721 cactagatcc aagcaataat tttgtcgtgt gtgccaatca gaacaccgat aatctgacat
    21781 tatatcgccg tcatcctgaa acaggattac tggaattact gaaaaaagat gtctatgtac
    21841 cagaatgtgt ctgtgtttat tttgatcgtt agtaagaaaa ggagaatgaa atgaatcagc
    21901 aagacgtatt agacagacta cgggaagaac taaatattcc tttctttgat gggaaaatcg
    21961 aagataaaga gtatagcgaa gaggaatatc agaaactcaa agccgattta cagaactact
    22021 ttgaacaata tgtacagaat gtagaaaact aatcagcttt ccatactatc tgaaaagaac
    22081 atcaggacaa gacttttccg tctatcctga tgttcttttc tacaaataaa aaaccgccga
    22141 gatggttaga ctcgacggaa aaggagtatt tactcgattt tgagtaagta caaggttgtt
    22201 gttggttata ggtatgtcat tatcataaag gattttgatc actctatcat aaaggtaata
    22261 ttacatttat attagataac tctagttttt attgttatca aagatttctt ttattttttc
    22321 aacaacaaat tagtcatttt ttggatggca gctgtcaatt ggtctgtcgc ttggtcttgt
    22381 tttgcttcag atgtattgaa tagatcttca tctacccctg attcaagatc gatactttgg
    22441 atcatttctt cacagcctgc tacatatgaa agataagctt tttcaaaatt tttatgaatg
    22501 cccattgctt ttgctggtgg acgcaaagta ctgactttct taagcattgc ccgatatttt
    22561 tttgttcctt cttcaaataa caggatgatc tcttcaagag tcgatttgtc cagttcactt
    22621 gtttttcctt catcaattgc gtttctgatc acttcatatt tcggatgcat tccttctccg
    22681 attgcttctg tttccttgac gatttgatcg atggactgca cataatagcc aatattcggt
    22741 aacatgttcg ttttcctact ttctccattg ttgttattat ttattctact tgtttttatc
    22801 ctcaaatgac aagtacttga aaaaatttaa tggtttctat acagctcgcc attcttattc
    22861 gtgctcaaag aaaccatctg ctaaactata ttgtttccct gcgacaatgt catattggat
    22921 caattgatat tcgtcgtaaa tctcttgttg cgagcgattg aattgcgctt ctttatacca
    22981 ctgtccgttt tgatagtaca gttctcgttc aaacgccgga agttcctgtt ctaatgataa
    23041 aagcagctga taaaagccat ttgttgtctg attcgtctgt tccattagat tcgctgcaaa
    23101 ataaaacgga ctggtgattg catcgtgagt cgtttgcttt tctaattctc ctttactgtc
    23161 gaacataaga aattgtgttt catgcaatgt ttgtttgtca ttttttgctt ggatcgtatc
    23221 tgaatagatc cctggaagat gatcccccca aaaaacaact aaagtccttc tagaaagatt
    23281 cttcaattct tccgtgaact gcttcagtgc cgtgctgcta taggaaatat cttgcagata
    23341 attctctaag tcaagtgtcc cacttccatc tgaagttttt gcaggatagt ctagattatc
    23401 atactttcca tcgtagggca tatgtgtctg catcgtgact aaatgaatga actgaggtgt
    23461 tttttcttcc ttgagaagtg tcattacttc tttgtaagct gactcatcag aaatatatgg
    23521 attattctct atcgtatccg tataggtcat cgtacgctcg ctgatgaact ggtcaaatcc
    23581 taatgtctga taaacatcct ctcttttata catcgaagta ttgtatggat ggatcgctgt
    23641 cgtctggtat gattgtgcgt ttaacgctga aacgatagaa ggaagctgat ccatttttgg
    23701 tacaagcatc gtgtaagggg tggtgagttg cgcattaaat aacgccatag aaaaaccagt
    23761 caatgcttca aattcgatat ttgctgttcc accgccatag ttttgagaaa gcatattgcc
    23821 actatatgtt tgatcagcta cttcataata atctgccaat ggctcaccag atacttctac
    23881 gccgtttaat cgactaggat ctgagaaact ttcactcatc acgaacacga tgttcggcga
    23941 ctcttcctct acagttttat ttttttcatc ggctagcttc tggtattttt cagtgatttc
    24001 tttgatcttt tctttcgaat atccttcagg ttcatccatt ggttctacct tcagattgta
    24061 caaaaaccca ccgataaaac cagtgttata gtagttcatt ttctggctgt aaggtatcca
    24121 tagtgccgtt ttattatatg cttttcgcaa caaattatcg ggattgttga aattactgat
    24181 ataccctagc agtccaattg ccacaagcag tgtcgttaat cggatcaatt ggaatcgcct
    24241 tccttttttc aggcttcgaa acatgtacca gcaaaacaat cctatcacgg ttcctgcggc
    24301 agctaagatc actgtaaaca gcatagtgcc ggtcatatct ttcagcaatc cgatttcagt
    24361 gatcatcttc aaatcgtctg gataaatcgg ttcttgccga taactcattt tcatataatt
    24421 tgcaaaacct aatacaccga tggctacgac atacgtaagg gaacctaaag ggacagatcc
    24481 tgctagtgca atcagaaaaa taagaataat cagcaagacg agacaggcta agaagaattt
    24541 ttccgtatgc caagaaaaag caaatttcaa cgcaaggtct acggatagat cattttgaca
    24601 ccactgcaaa aataaattac taaaaacaac aagaaaaaag atactgatga ttgctgctgt
    24661 tccttttaaa atctttttct gtttggtcga aagtctgctt atatatcttt tcaaatgctt
    24721 gctccttcat ttctatcaat aaaagaatgt tgcttctctt attttagttg tacgaaattc
    24781 gaaagtaaag aacggaaaga agaattaatg aaatccaaat attctctttc tttatggcct
    24841 ttctggtttc tttaataagc ggacctttcg tactccagaa attttttcta gctgttgttt
    24901 cacttgattg agctgttcaa aatcagtact gtctatgtca atcaatatgt agacatgctc
    24961 attcttatgg ttacgcgata tatctgcgat cgctagctcg ttctcattca gcagagaaaa
    25021 tatctctgct aatatattgg ttttcttttg ataaaatata gtgaatcgaa acggcgcttg
    25081 gaaaaacatt cgtgcggatg gataattgac cgcttctcta accgtaccga acaaaagata
    25141 atcttttaag gcttcaactg ccaaacgtcc gctgtctgcc aaagcttcct gagtcgatcc
    25201 ccctagatga ggcaacataa gtatacggtc attttctaag aactcttctt ctggaaaatc
    25261 agtcaagtat ttagccagat gttgtttttc caatgcttgc ataacagcat atttatcaac
    25321 aatttcactt cttcctacat tgataagtac agcatctttt ttcatttttt ctatattcgc
    25381 ctgatcgatc aatccttttg tatcttctgt caacggcagc atgactacga tgtaatccga
    25441 ggtagaaaga actgtttcta aatctgcttg ccgaacataa tccaactggg catcgcggat
    25501 agaataaccc aagacatcca tccctaagct atagcaactt agcgctactt ttgtgcctat
    25561 cgctccaaga ccaagaagac cgatggtttt gccttgcaat tctcgtccca cataagcact
    25621 tcttttgttt tctgcctgtt ctaaaatatt aggacctgtt agtgtttgta ccatacgact
    25681 tgcttcgatt atcggacggg aagataagag caaacagcac aaaacaagct cttttactgc
    25741 attagcattt acacctggtg tattcatgac gatcgtcccg ttttcagaag ctttttcaac
    25801 gttgatcgtg ttcaccccaa caccagctct tgaaattgct agcaaatctg gtgtaagcca
    25861 ttcatctttc actttcgtac tacgaataac tattccttcg gctggcaaat catctattat
    25921 tttgaaatat tccccttgta acttttctat ctcttgttct ggaatagcat caatcaaatg
    25981 aacgtgatac ataaaacccc ctccttacta acaagtatct cgaaatttct gcaattagac
    26041 aactgtttac catttgactt cctagaagca aaaagagcag ttagagcaga gtaggctgag
    26101 tgataaaaat aaggctatga caagttactg ccataagcct tattttcgag agcaaaatat
    26161 tgagggtgag atatcttgat aaatggtgtt ccgttagctg acattgataa taagactaaa
    26221 tttcaaaaaa gttgaaaagc aaatttcgtc aatctcttct tatttcttaa aactaagtgg
    26281 ctttttcaca acctcttttt atctaataac cactgtttcc aactcaacta attttgccca
    26341 cgtttcaatt tgatctgttg ttagatttag cgagacaacg gtatgatgac cgccacctgc
    26401 ctgaatccat gaatggacac cttcatggaa attcggttta gttttccata acacacgtgc
    26461 aacaggtaaa ttaggtgctt cagtcgttgg ttcaaaggct tctacttcat tgatcagcag
    26521 tttataatgc gtaccgaaat ctgccatcga tacgactacc ccttctcctg ctttcccatc
    26581 aaatacaaga cgcgccggat cttctctatc tcccattgat aaaggagaaa caacgattct
    26641 tggtttcgtc gcagcaagcg tcggatctac ttccatcata tgggattcca agattgcttc
    26701 ttggccacta gctagctcgt aagtataatc ttccatgaat cctgtatctt tccctcgagc
    26761 catgattttc agcaaccgat caatcgctgc agttttccag tcgccttccc cagcaaaacc
    26821 gtatccttct gccatcaaac gttgtacagc tagtccaggt agttgtttca ttccatacag
    26881 gtcttcaaaa ttagtcgtaa aagcgttata gcctccttct tctaagaaac ggcgaatacc
    26941 gatttcctgc tgagcttgta cttttacatg ttcctcccat gtcttttctt cataatcacc
    27001 atagtcgaag gtatacaatt ccttgtactc ttcaaataat tgatcgactt cttcgtctga
    27061 tacgcgatcg attacttgta caagatctcc gataccaaaa taatcaacag tccaaccaaa
    27121 ttgaatctgt gcttccactt tgtctccttc tgtgacaccc acgtttctca tgttgtcacc
    27181 aaaacgagcc acttttattc caaaactttc attataggct actgcaacat ccatccaatc
    27241 agcaatctct ttttggatct caggtcgttt ccagtagcca acgacgattt tattttgttt
    27301 atttaatcga gcattgatga acccatattc gcggtcacca tgagcggatt gatttaaatt
    27361 catgaaatcc atatcgatcg ttttccaagg aatgctttcg ttatattgcg tagctaaatg
    27421 aagcaatggt ttttgtagaa gctttgtccc gcggatccac atttttgctg gtgagaaagt
    27481 atgcatccaa gtgatcacac ctgctacttc ttctcgataa ttgacctctt tcatgatttt
    27541 tgtgatcgta tcagcagtaa ctgctaattc ttgaaggcgg attggataag gcaattggcc
    27601 attttcattc agttcatcga ccatttcttg tgcatgtttc tttacttctc gcagtgcttc
    27661 ttccccatat aaatgctgtg atcctacaac aaaccaaaat tctttttctc caatattcaa
    27721 cataattagc cactcctttt atttgttttg tccgtaatac gcattttttc cgtgttttcg
    27781 taaataatgt ttatctaaaa tccgttgagg cagctcttct gaaaatgaat tgagctgtct
    27841 ggtaaataaa ttcatcttgc atacttcatc tagtacgact gcgttcatta ccgcactttg
    27901 cgcatctttc ccccaagtaa atggaccgtg tccgtgtaaa agtacccctg gaattgctaa
    27961 gggatcaatt ttgcgttctt tgaatgtttc tatgataact tttcctgttt catattcgta
    28021 cccgctgtcg atttcctgtt gtgtcaaaaa tcgagcacaa ggtaccgatc cataaaaagt
    28081 atctgcatgc gtcgtcccca tcgcaggaac atccaatcct gcttgtgccc agatcgtcgc
    28141 ccatgtcgag tgagtatgga cgattcctcc gatttgagga aatgaacgat ataaaacagc
    28201 atgagtaggc gtatccgaag agggattgag tttaccttca atgatatgat tgtccaagtc
    28261 cacaacgacc atgtcgtctg ctgtcatctc ttcataactg actccgctag gtttgataac
    28321 gaaatatcgt gtttcaggat caaaagcgct cacatttccc catgtatatt tgacgagtcc
    28381 gtgtttaggc aagtcaagat ttgcttggta tacttcttct ttcagttgtt ccagcatttt
    28441 ctatcactcc tttttcttgc aattgatgaa tcgctgtttt ttcaatgtcg atcccctcac
    28501 ggtaacgtct cataaatatc tgatagcctg ctatttcttc tgctgaaggt gtgatggtga
    28561 ttccttggtc ttgtccaaac acgtcttctg ccaagaagtt ttctaaagac ttcccatgtc
    28621 gatccatcat ataggctgct aataacgcga ttccccacgc tccaccttct ccggctgtgt
    28681 ccatcactgt aactggtgcc tccatagcag atgctagaat ccgctgtcca acttctggtg
    28741 ttttaaaaat ccccccatgc cccactaaac gatcaatttg gacattctca gatttcaata
    28801 tatccattcc gattctcatt gcaccaaaag cactggctaa atggatccgc atgaaattcg
    28861 ctaaatcaaa acggctacct ggtgtacgta caaataatgg ccttccttca tttactcctg
    28921 taatgttttc accggaaaaa taaccataag aaagtaatcc cccgcaatcc gtatcgcctt
    28981 ctagcgcttt gttgaataat accgtgaata atttatctct gcttatctcc atacccaaag
    29041 aattggtaaa ttcttcaaaa atactgaccc aagcattgat atccgaagag cagttattcg
    29101 tatgaaccat gcctactagt tctcctgaag gtgtagtgac catgtcgatt tccggatgga
    29161 cattttctag ttctttttct aatacgatca tagcgaaggc ggaagtacca gccgaaatgt
    29221 tccctgtccg tttggctaca ctgttcgtag cgaccatacc ggttcctgca tccccttctg
    29281 gcggacaaac cgggattcct ggtctcaaat ttcccgtagg gtcgatcaga taagcccctg
    29341 attcgcttaa cttgccagct tcttctcctg ctactagaac tttaggcaaa atgtgatcca
    29401 agtcaatcgc tacgtgcgct ttttcgatta actgaccaaa aatatttttc atttcttgat
    29461 ggtaggtttt cgttgaagga tcgataggaa acatgcctga tgcatctcct acgcctagta
    29521 ctttctcacc agtaagctgc caatggacat aacctgcaag cgttgtcaaa aaattaattt
    29581 ctttcacatg ttcttcttta ttcaaaatag attgatacaa atgagcgata ctccatctct
    29641 gcggaatatt gtattgaaat ttttccgtta atattcgttc tgcttcttca gtgattgtat
    29701 ttcgccaagt acgaaatggt actagtaact gttccttgct atcaaatgct agataaccgt
    29761 gcatcattgc actgaaccca atcgacccga ttgtcgtaag attctcgcca tagatttcga
    29821 atacttctgc tgccattttt cggtaactga cgcgcagtcc tttccaaata tcttctaatg
    29881 aataggtcca gataccattt tctagtttgt tttcccactc ataactacca gcggcaatag
    29941 gagtaaaaga tgaatcaatc aatacagctt tgatcctagt tgatccaaac tcaatcccta
    30001 aagatgtacg accatctatg acatctaaca tactttgcgc atttacttcc ttcataatga
    30061 tttaactccc tccttctcgt caaacctgat caaaccgtct ttttagtagc gcttacaatg
    30121 attattgtat acttttgtac gtacatgtca agcggttatt tacaagaata caataaaaaa
    30181 cacttgtttt gtacgtacat aagttataaa atgaaattgc ttacaaaatc tgatcaaccg
    30241 ccttctcgaa attctagaag gagagaatac ttatgaaaaa agaaaaaaaa gtttcctcat
    30301 cgtttattta tttttttggc gcatttggcg gcattctatt tggatatgat attggtgtta
    30361 tgaccggcgc cttacctttt ttacagcatg attggggttt ggcaggtaaa gcttctttga
    30421 taggatggat cacgtcttcg gtcatgttag gtgcgatcct tggcggctct ttatctggtc
    30481 ttctttctga taagcttgga cgacgaaaaa tgattcttct ttctgcactt atttttatgg
    30541 ctggttctgt cctttctgca tctgcccctc acaatggttc ttattttctg attgcagcaa
    30601 ggattcttct aggtttagct gttggcgctg catctgccct agttcctgct tatatgtctg
    30661 aaatggctcc tgctagacta aggggaagat tgtcagggat caatcaagtg atgattgctt
    30721 ctggcatgct cctttcctat gttgcagatt atttgctaaa agtgttgcct gaaacgatgg
    30781 cttggcgcgt gatgttaggg ctcgctgctg tccccgcgct gattttattt ttcggtgttt
    30841 tagctctacc agagtcaccg cgttttctta tgcaatctgg ccgcttggaa gaggcaaaaa
    30901 gagtattgaa ctatattcgg acaccaaaag aagctgaaca ggaattcgaa caaatccagc
    30961 tgaatgtcaa acaagaaaag acaaccggaa cttcttggca tactttgttt ttagaaaaat
    31021 atcgttcctt agtcattgcc ggtattggtg tagcagtatt ccagcaattt caaggagcta
    31081 atgctatttt ttactatatt ccgctgatcg tagaaaaagc gactgggcat gcagcgagcg
    31141 atgcattgat gtggccgatc atccaaggaa tcattttagt tgctggttct ttgctctttt
    31201 tggttattgc agacaagttc aatcggcgaa ctttattgaa aataggcggt agcgtgatgg
    31261 gactttcatt catcctacca gctgttttag gaactgtact ggatgctcat acaaattcgt
    31321 tgttgattct cttgttttta tgtatttatg tcgcatttta ttcttgcaca tgggctccac
    31381 tgacatgggt aatcataggc gaaatattcc cactagctgt ccgcgggcga gcatctggcc
    31441 tagcctcttc ttttaattgg attggttctt tcctagttgg cttgcttttt cctgtgatga
    31501 ctgcttctat gtctcaagaa atcgttttcg gcatttttgg gatcatctgc tttttagggg
    31561 ttttatttat tcaagagatc gtaccagaaa ctcgtgggaa atctttagag gaaatcgaac
    31621 aaagtgcatc taaaaaaact tatcctaaac gtatttctat ccatcattct taaagaggtt
    31681 gtgacagaag tggtcagctt caagaaataa gaaggaaccg cttagtttcc agtcacaagg
    31741 aacaatttca aaaaacagct tctcatgttt gatgaatatt gtgacttgtg accagttggt
    31801 aggagaacac cgtttagtgg agaggtggtg aaagaaacgt tcagccttga gcgatgacaa
    31861 aagctctcac aaacattgcg acgagtaacc gtaggagcaa taagaggcgg taaaaacccg
    31921 tctcttattt ttcgttttct ttcactgttt cccgttcaac tagtacaggt tcatagagaa
    31981 tatttggctg tgcctgtcct ttttcaatcg ttgtgacgat ccaccgtgcg gcatcttgtc
    32041 ccaacttttc ttttggatgc gtcaatgttg tcaatttttt attccctaaa tggcttaaag
    32101 aagaatcgtc tgttcctacg atcgataggt catctggaat atgatagcca ttttctgtca
    32161 acttatctag cattaaacta gctatttgat cgttatagca aatgatgcca tctaaagctt
    32221 gttcttcttt caaccgttgt actgtttttt cagcgattgt ttctcgtgtt tccgtggtga
    32281 aagtaatgat atcttccgtc aaaaaacgca cattatactg ttcacacgcg ctgataaaac
    32341 ctttcatcct gtattttcct tgaagatcgt cgatttttgt gatgaacaac agacgctgat
    32401 gtcctttttt tatcaaatat tctgctgcca taaaaccgac tttcacatca tctaaacaaa
    32461 tataagaggc atttaactct tcataagcag cattgatcat caccatcgga atgccttgtt
    32521 ctctcaaagc aacataaagt gctaagtttg ggttatactg attacttttc gtaggctcaa
    32581 cgatcaaacc atctacccca taagcaatca ttttcttcaa acactctttc tccaattcgt
    32641 gatcgttatt cgtactggct aaaagcaatg aatacccaga ttctcgtaat tcattttcga
    32701 ttcctcggat aatcgacgga aaaatataat cagagagata tgtggtgatg acaccgatcg
    32761 tttttgttct gttcaatggt gttttttctt tcttatagct gtcatctaca tatgtgcccg
    32821 accctttttc tttacgcaga tagccctcat ttaccaacac agcgattgct tgccgaatcg
    32881 tatgtctgct gacgtcatat atttcttgca gcttgaattc tgagggaatg gttgtattta
    32941 cttcgtattc ctcacgaagg atcttttgtt ttatctcttc tgcaatatct tgatattttg
    33001 ccatttgcct tgcccctttt tatttgtacg tacatatatc ataacgagtc tgcaaggttt
    33061 attcaagcaa aaatcaaggt ttcttgtgct cataaggcat acggaacagg ttcaccaaat
    33121 tctggaaatc cttgctcatt ccacgtaaag cattgcgcgt tcgcgtgccg attaggattg
    33181 tctaacggat cccctttttg gttcttctct ggtcgagcat gatagatcaa aatatcaaag
    33241 cacccgtctt ctgaaaccgt aaacgagttg tgaccaggac caaattgctt atttttttca
    33301 gaagaaacga atactggctc cgataattta tgccatgatt cttttaataa aagattcttg
    33361 tgctcatcag cccacagcag gcccatggca tagttttcat ctgttgcact accagaatac
    33421 gtaatgaata ttttcccatt gcgagtaagg actgctggac cttcatttac tgaaaaaccc
    33481 cgtttttccc attcatactc aggtatagaa aggaggatct gcttcccctt taacgtccaa
    33541 ggattatcca tttcggatag ataaagatta gaattcccag taatttcagg atcttgttgg
    33601 gcccacacat aatatagctt tccttgagaa gagaaagttg ttccgtctaa actgaaagtt
    33661 tcccttttcg tttttatttg tcccttttct atccaatctg tattcattgg atctatatgc
    33721 tgattttcaa gtacgaacat gcggtgatga tgagattcat cacgtatagt tttatgattg
    33781 cttgcagcaa agtaaatata ccattttcca ttgatataat ggacttcagg tgcccagatc
    33841 aattcactca tcggtccttc ttcatgagcc tgccatacaa tcaggcttgc agcatgttct
    33901 aattcattca gtgactttgc ccttcttaat tcaatcactt ggtagcctgg aaccgatcct
    33961 gtaaaataat aataaccatc tgtatgttta tatacccaag gatctgcccg ttgccgaacg
    34021 ataggattat tataaagtgt cttcatcata acaaatttcc tcacattttc tttattctaa
    34081 ttacattcca agagtatttt tctatcccta catttaattc gtcatgtttt tctattgaaa
    34141 aatagtttat ttcaggtttt attttttctg gttcttcacg tgtatttttg tccaacagat
    34201 cgctactggt taatgtgtag tgctctcctt gatctagcaa attgaaatca tgttcaatcg
    34261 ttatcttttg ttgttcattt gtgcgattga tcaagaaaat agtcagctga ttgccgttat
    34321 ctacgatcac actgtccaca taaggaacat tttttatctt ttcacaatca taaacagggc
    34381 aaatgatttg ttgtttcaat acttgacctt tagcatattt cgataagtgt tgcatcacat
    34441 aataagttgg attcctccat gcttgtcctt tagcatctgt cagtattaaa ggtattgtgt
    34501 ttactaacaa tgattgacaa ctaatcttta cgcgatctgc gtgtcgtaaa atagccagcc
    34561 ctaatgaacc aaataataat gtatcttcca tagtaaagta acaccaatcg atgggactac
    34621 cgacttcaaa atccctatgt cttttttccg gttgggcatg tacattccat tcatcaaacg
    34681 ctagatacat tgttttctca cttctcaaat atccttttac ataatcacaa gtagcaacga
    34741 tttcttcaat ttgacgatcg aatcgataag cttttgcaag gtatgtcgct gtatcatcac
    34801 gctcattttt aggaggttta gtcaagtctt cttcatcata cttatcaata tagttatgca
    34861 aagctaaata atctacattc tcataagctt cttctaatgc gatacgatcc cattcagggt
    34921 acgatggtaa acgtggcgta gagcttccaa ctaagattgt ttcaattgaa tcatccacta
    34981 acttcatgac ttttgaggct tcatttgcta acctaccata ttcataagca ctcttagccc
    35041 ccacttgcca tggaccgtct agctcattcc ctaaacacca taatttaacg ccaaaaggct
    35101 ttttttgccc attttttatt cttaaatcac tccagaaggt tccttttgga aaattacaat
    35161 attctactaa attttttgct tcatcaattc cttttgttcc taaattcacc gtgaatatcg
    35221 gtttcgcttg tacatgactg atccatttaa aaaattcgtg gagtccaaaa gaatttggtt
    35281 ctatacctcg ccatgctaaa tcaatttttt ttggtcgttg ttctttccta cctacagtat
    35341 ccacccaatc ataaccagat gtaaagtttc ctccaggata gcgaatgaca cctaattcta
    35401 attcctttac ataagagagg acatcttgtc taaacccgtc accatcagct gtttcatggc
    35461 ccggttcata aatccctgta tagacgacac ttcccatatg ctcaacaaat gagccaaaca
    35521 gccgagaact gatctctcct accgtaaaat cctctctgat ttttactttc acttcttcgt
    35581 ccaacagctt tccctacctt tcatttccta tagtcaagat aatagataca agtttccata
    35641 cgccaccagc aaagataaaa ataaaaggag ggaaaagaaa tatgagcagt acaaatccta
    35701 ataacaatac gcaacaaaga atatttctca aaaaatcaaa aaatactttc catgcacttt
    35761 gttcaaaaat ttgtaaaaat gtgatatctt tgttttcaga aatcacatat atccaggata
    35821 aaagaaacat ggaataaata agtaaaacta catatccact aattgaaagt acgattgtct
    35881 gttcttctaa taattgatta acaagaaaat aaataatgat cgttaacaga ctaaggaaca
    35941 agttcttacc aaaatgttgc tttattcctt gaaagaaaaa ataaatgata tttcctgatc
    36001 cctcaccttt ccattgcgcc attacataaa aaacagcaca tgtagcaggc acgatagtaa
    36061 taacaggcaa acacacgatt acccagagaa tacctaataa gatgtaatca gaaaccacaa
    36121 tcaactgtcc ataaaattta gtctcaacta ttttcatctt cctcactctt tcattccaga
    36181 catagctgtt ccttgaacaa aatatttctg tgcaaaaata taaaccacta gtaatggcag
    36241 cattgaaata accgttccag ccattacagg tccataaaaa gaaatgttct gccctgtaaa
    36301 taaagctaac cctgctggta aagttctcat ttttgaacta cttgtcaata tcaatggata
    36361 aagcaagtca ttccaactat tcatcagcgt gaaaatccct aatgataaaa gagtaggttt
    36421 gctcaatggc aacatgattt tcaaaaaaat attaaactca cttaatccat caatgcgtgc
    36481 tgcctcttcc aaagaatctg gtaaagtaat aaaaaaggaa cgcatcatga agataccaaa
    36541 tgcagttgtc atacgaggaa tgatcaaacc tgcataagta tcaagcatgc caagcaaatt
    36601 gacttcaata aatagaggaa tcatgaagac ttggaatggg atcatcattg tcagcaaaac
    36661 gaaatagaac aaaactgatt ttcctttgaa acgcatacga gcaaatgcat accccgcaag
    36721 agaatcaaag aatacagaag tgatcactgt ccctccagca aaaatcactg tatttttgat
    36781 gtaatctaat aaagggatcg ttgtccaaac cttaatatag ttatctaggg tatagctatc
    36841 tgcaaatatt ctaggcggaa atgagataat gtctttttcg tgtttaaaag aagaaataat
    36901 cagccacaaa aaaggaaacg caataaccaa caatataact agcatactaa tcgttcgaat
    36961 gattttttta ccactaactg cttttcgact attcacagta actgtaatcg agctttctgg
    37021 tgccatacag gtttccctcc tatttcattt gtttttcttt ccgtgacatg tacacattag
    37081 tggagatagc tatagttgca acaatgataa acaaaaccga agacagtgct gatgcatagc
    37141 ccaattcata aggtgctgta aatcctcgac tgtaaatata ttgaacagct gtttctgtct
    37201 taaattgtgg gccaccagcg gttgcaacat atacttggtc aaacacttgc atcgaaccaa
    37261 tcagattcgt taaaatacaa aaacctaagg agggaacgat ctgaggtagc gtaatgttaa
    37321 aaaactgctg tcgatttgtt gcaccatcca tttgagcaga ttcatacaaa gagggagaga
    37381 ttccctgaat agcagtcaag agaatcgtca tagtaacacc aaaattcttc caaacagtca
    37441 tgatggctac cgtcggcata gcaagggttg gatcgcgaaa aaattgaggg atcggcaagc
    37501 cgacttgtga aaacaagtaa ggaatcgccc ctacattcgg atcaagcaac atagaaaaaa
    37561 taatcgcaat tgacgtcaat gaacaaataa ctggaatgta aaatgtcgaa cgacaaaaac
    37621 gattgaatct ggtattttta gataatgcta ctgctgctaa taaaccaacg ataatttgtg
    37681 ttggcgtttc gagtacggtg aaatataaag aatgaatcaa cgaattaact gctcgttcat
    37741 cactaaatat acgtgtgaaa ttttcaaaac ttacaaaatt tcttgatgta aagaaaatat
    37801 tcaaatctgt aaaactaata gcaaatgttc ctaaaagcgg tatgacagta aataataata
    37861 aaactaaaaa agatggagtt aaaaacaaat acgcagcacg atcaggacga ttccaatatt
    37921 tggtgaatcc gttcatatag tccacctcat ctttcatttt gataaaatat acaatcttgg
    37981 tttaaacgaa tctgaataag atatccgaac aatcgctgtg atttttgcaa taacacctat
    38041 ctgcaaatac taggtcgatc attcagatat ctgtatcttg ccaacagtct atcctgtata
    38101 aagttctgta ctatttttgc agtaattgat tgatggcatc atctgcatcg ttcatgagtg
    38161 tctcaggatc ttctcctgct aataattttt caatcagtgg attgatgata tcgttattga
    38221 tcgcagagat ttgggtaaaa cctggtaaat atggctctgc atagtccatt tgtttcgata
    38281 attcagatac aataggatca ttttttactt cttcatcttt tgctactgaa tccaagtagg
    38341 caggaaaacc attttcgata ctccattttt taccaatttc agttgtattc caatatttca
    38401 aaaactcata gacagcttct tttttattat tatctgtact tgccggaatg gcatatccta
    38461 cgccatctaa aatagctgtt tttttgtctg aatcactccc ttgtggtaag gtagtcacat
    38521 tataatcaat ctcgtttgct ttcaatccgt tatttaacca tggcccattg atttcaatgg
    38581 ctagttgacc agcattcatt aaattgtcca tttcggcgcc agaaatcgat tctggaccag
    38641 tcttgtctac atggatcatt ttttggatcg cttttaacgt tttaaggttc tcaggagacg
    38701 caaattcaga cttagtataa tcatcgttca ctaatttacc gccatttgct aacatccagt
    38761 tatataacgg tgcagttcca tccttattga agacaaaacc tgatacattt ttactactat
    38821 cagttaattt tggtgccatt tctgtcaatt cttcccaagt agtaggtggt tgttcagtat
    38881 ctaatccagc ttgtttaaac aaatcattat tccaatacat atacatgcct tgtacctgca
    38941 tggggacaaa atactgttta ccttctactt cacctaattc aatagccgtt tctttaaaat
    39001 cagatttatc tactccatca tattcccaga aatcgtctaa tggttgtacc gctccgtttt
    39061 ctacatactg tgcaaaatct ggataattta atgcaataaa gtcaggtgct tttttagaag
    39121 aaatcgctgt aggaagtttt tcattcaaat tggcccaagt catgatatcc atttcaattt
    39181 tgatgttttt atcatttgtt ttattaaatt cattaacgat ttcttgcaat atttcaccat
    39241 cagatgcagt aaacccattc caaaaagtca aagtcacttc tttatcagaa tcagattctg
    39301 ctgaacttcc gccaccgcaa gctcctaaaa tacctgttgc agctaccaac aaagagccaa
    39361 ttaaccattt tttacctttc attttttgtt cctccttcat ctgataaacg acatctgacc
    39421 acattccaag atagcggttt caataaacaa ttgattttcc catcttcagc ttttacattt
    39481 gttgaatgac ttggttttac ttgttcactt ttaggaagat tagttgcttt tgtatcaaat
    39541 ccactcattt cagaaaaatc aatcactccc tctaatgata attcagacaa gtgaacagtt
    39601 agattcattt cttcttctcc acggttaact gcaaatatga caagttcttt gtcatttatt
    39661 acggctatac tttctaaata cggcacttct tcaaaatctt ccgtcgcata gtttgctgaa
    39721 cgaatttgtg ggcttaacac ctctccttgt ccatacacag cagcttgcat aaatggataa
    39781 tagatcgtct gtctccaagc ttctccgttt gtttctgtcc tgattggtgc aataacgttt
    39841 accaattgag caagacaagc aatctttact ctatctgcat gtttcaataa agtgattaat
    39901 agacagccta atagtaatgc atcttcaaaa ttatagttat cttctagtaa aggaggagca
    39961 atctgccaag gcgctgcttg ctcatcttgt tccgctgaat gataccaaat attccattca
    40021 tcaaaggaca ggttgatctg cttcttacta tgtttcttcg ccttgactgt atcgcacatc
    40081 gctgtcactt cagaaatgaa ccgatccatg tctaaggaac gtgcaagata atgagaaaga
    40141 tcattttttt ggttgccata atattgatgc agagacaaat aatctatttg atcgtacgct
    40201 tgatcaagaa ctgttaactc ccaatcacca aatgtaggca tttgccgatt cgaacttccg
    40261 cataaaacca cctcaattga atcatctacg agtttcatcg ccttagccgt ttcctctgct
    40321 aaccgtccat attcttccgc tgttttatgt ccaatctgcc aaggaccgtc catttcattc
    40381 cctaaacacc atgtcttaat tccaaaaggg ctctcttgac cattcgaacg ccttaaatca
    40441 ctccaataag ttcctttagg aaaattacaa tattcgacta aattacgagc tgcatctact
    40501 ccacgagtac ccagattgac agccatattg atttcggccc ccacatgctg acaccagtta
    40561 taaaattcat gaatcccaac ttcattcgtt tcaattgtcc gccaagctaa atccaacctt
    40621 ctaggtctat ttgctatggg cccaatactg tcttcccaac gaaatcctga cacaaaattt
    40681 ccaccaggat atctaatcat gggaatgttc aattctttta ccaagtgaat gacgtcttgg
    40741 cgaaatccac ttttattagc tgatggatga tctggctgat aaatcccttc atatactgct
    40801 cgtcccatat gttcaataaa tgaactgtac aaccgcggat caactttgcc tacacgcttg
    40861 acactactaa tcaacattgt ggcttccata actgacctcc tttaatttat acatgaataa
    40921 tacacctcgt tgaaagcgtt gtcaataata taaatgttaa acttacttat tttttatcga
    40981 ttttaattaa ccaaaatatt aataatgatt aaaacaaata atttaatata acttttatta
    41041 acaaatatgt taatatataa gaaaaacctt ttaagaggtg atatatatgc aactacaaat
    41101 aaacgaagaa tccttaccgg tatatgaagc attagctagt aaaacaagga tcaagatcat
    41161 ccagctactt tccaaaaaaa agatgaatgt caaagatttg gcaaaagaac ttggagtgag
    41221 cagtgctatc acgacgatgc acgtgaaaaa gctagaggaa gcaaacatca tcaaaacaga
    41281 aaaagtagga caacaaaaaa tttcgagcct tcgtgtcgat aaaatcgata ttagttttcc
    41341 agaaaaaata ttcaatgctt ttgatacgaa agaaacatcg atacctattg gtcactatac
    41401 aaattatgca attgaaccaa catgcggttt agctacgatc catgatttta tcgggaaagt
    41461 ggatgaacca cgctatttta tggatcctcg aaggatggat gcccgcattc tctggtttac
    41521 cagcggattt gtcgagtatc aggcacctaa ttttttaaat acagaggata cattggagat
    41581 gttggaagtt tctgtggaaa tttcttctga atttcctttt tcaaatgaca attggccttc
    41641 agatatcact ttcagcctaa atggtgtgga gctaggaaca tggacgagcc ctggagattt
    41701 tgctgatatc cgcggtaaat atacgcctga ttggtatcca gataatctta atcaatacgg
    41761 cttgctgaag acgatccgaa ttaccaaaca cttgacgaat atgaatggag aacccttgtc
    41821 aaacatcacg atcaacgaca tccctaaaga gcaagacact tggcatttgc gaatcgaagt
    41881 gaaagacgat gccaaacatg tggggggctg tactttattt ggtaaaggat tcggtaatta
    41941 cgatcaggat atcaaattaa aagtttacta cagctgatat ttttcttttc tattttataa
    42001 aacaaaaaca gccaatccct atgaagggac ggctgttttt gctttattgt tatttccaga
    42061 aatcatccaa aacggaaatt ggcaaatgtc gtttatgttg tgtcttgttc caccatgctt
    42121 cgattttttc ttgtgcttga gcgggaactt cttttccttc tagataatca tcaatttcat
    42181 tatatgtgac acctagtgcg acttcatctg caatcattgg tttcccatct tcaagatctg
    42241 cagtcggaat ttttgtgtat aatttttccg gtgcattcaa tgcttgaagc aattgtttcc
    42301 cttgtcgttt gtccaaacgg aataatggca aaatgtcggc tccaccatca ccaaatttcg
    42361 tgaagaatcc agtaatgttt tctgcagcat gatctgtccc taatacggct cctgctcttt
    42421 ctccagctac ggcatattgc gtaatcatac gttgacgtgc tttgatattt cctttgttaa
    42481 aatcgctgat tcccacacct gcttcgctta atacttgtac ttgtgcatca acagctggtt
    42541 tgatatttac tcgcagacta acatcgggct ggataaagtc aagagcagct tttgcatctt
    42601 cttcatctgc ttgttcacca taaggaagac gaacagcgat aaattgatag ttttcatctt
    42661 ttgtttcttc acgcatttct tccatcgtta gttgcgccaa acggcctgct aaagtagaat
    42721 cttggccgcc gctgatacct agtacaaaag ttttaagaaa cggatatttt ttcaaatatg
    42781 ctttcataaa gtcaatactt ttacgtattt cttcttttgg atcaattgtt ggtttgacac
    42841 ctaattcatt gataatttct gtctgcaggc tcatttattc tcctcctatg cttctgtatg
    42901 aagttctgct acgctttttt tcattcgttc catgatagcc attttatggt tccagcattc
    42961 agtagacaga tcgactgggt atttttgcgg attcaagtct cgtttatact cttcccatag
    43021 tgaatctaga ttttcttttg tatattcttt gatttcgttt agtgcaggta attcgtaaac
    43081 tctttttcct tcgataaaga tatcttgtaa aactgggcga gctgtaaagt ctcgaacagt
    43141 tttattgata aacgtatgca ctggatggaa catgaagata gcttcttctt ctcgagggtc
    43201 ttcatcccat aatgtcacgt agtctccttc agatttccca tcaaaatttc gagtgatgcg
    43261 ccacacttgt tttttccctg gagtagttac tttttctgca ttgcttgata acttgatcgt
    43321 atccatcatt tttccttcat catcttcgat cgatactagc ttgaatactg caccaagggc
    43381 tggctgatca taggctgtga tcaattttgt cccaacaccc catacatcaa ttttagcttt
    43441 ttgcatcttt aaattcaaaa tcgttgcttc atccaagtca ttcgaagcat agatttttgc
    43501 ttccgtaaat ccggctgcat ctaattgttc ccgaacacgc ttagatatat aggccatatc
    43561 tccactgtcg atgcgtacgc cttggaagtt gattttgtca cctaattctt tcgccacgcg
    43621 aatcgcactt ggcacacctg atttcaacgt atcataagtg tcgactaaga acacgcagtc
    43681 tttatgggtt ttagcgtagg ccataaatgc ttcataatca tttccataag attggactaa
    43741 cgaatgagca tgggtcccgc tagctggtat cccaaatatc ttcccagcac gtacattgct
    43801 tgttgcatct gctccgccga tatatgccgc tctagttccc cagactgctg catccagttc
    43861 ttgtgcccgt ctagttccaa attccaatag tggatcttct ccgatgacag atttgatacg
    43921 agctgctttt gtcgcgatca acgtttggaa attgaccata ttcaacagtg ctgtttctac
    43981 taattggcag tgcgctaaag gcccttccac ttggatcaat ggttcatttg caaaaactag
    44041 ttctccttca cgagcagaac gaactgttgc tttaaattca aaattttgta aatactccaa
    44101 aaaaccttca ggatagactt ctaatccacg caagtaagca atatcggaat cattgaacgt
    44161 cagattctct aaataattga ctaaccgttc caatcctgca aaaaccgcgt atccgtggtt
    44221 aaaaggcatt tctcgaaagt aacattcaaa aacagcatgt aaatcagctc gacctaattc
    44281 ccagtatgtc tgcatcatat tgatttgata tagatccgta tgcagcgtca aactgtcgtc
    44341 tgggtaagta ttattcatct tgtattactc ctcttggcaa gttttcattt cctacccatt
    44401 ataacaaaaa aaattaatag gaaaagaaat cttccatttt ttctgaaaaa gcacatgttt
    44461 tacggatatt tcttgctaaa aatgaactcc atctgctaaa actatccttt cttattggta
    44521 tttaacctaa acgagaaaga atacttattt tattcaatgt atccgagaca cgaagaaatt
    44581 agcgttttct atttttctta cttgataaaa taagtagatt ttcctactac aatcgtagcc
    44641 gttaagatac cgatgattga acaaatataa ccaaatgtct ctacaacata atcaatggca
    44701 aaagtttttg aagcaataat acgtctttcc ttttttgctt cttatcccca aagaaacgga
    44761 tataacttat tatgtattga atttttttgg aaaggcgaat tatgacaaga tataccttgc
    44821 gttgaaaaaa ctcgcggaaa aaatatcaca cgtttttaaa aagatacaaa gtaagaaagc
    44881 agctgtcaat agaatggaaa caaaaatggc gagacatcaa tcagtctcgc cttgtttt
//
LOCUS       DACDXO010000017        44677 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E23605_2017
            SAMN12639989-rid9881463.denovo.017, whole genome shotgun sequence.
ACCESSION   DACDXO010000017 DACDXO010000000
VERSION     DACDXO010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639989
            Sequence Read Archive: SRR10023389
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 44677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741914.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:42:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,972
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 33 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 17 of 73
            Pseudo Genes (multiple problems)  :: 10 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44677
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639989-rid9881463.denovo.017"
                     /isolate="E23605_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            23..646
                     /locus_tag="IWM35_001099"
     CDS             23..646
                     /locus_tag="IWM35_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9918965.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            1096..2145
                     /locus_tag="IWM35_001100"
     CDS             1096..2145
                     /locus_tag="IWM35_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP9918966.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            2142..3023
                     /locus_tag="IWM35_001101"
     CDS             2142..3023
                     /locus_tag="IWM35_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9918967.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            3026..3796
                     /locus_tag="IWM35_001102"
     CDS             3026..3796
                     /locus_tag="IWM35_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP9918968.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            4075..4929
                     /gene="aroE"
                     /locus_tag="IWM35_001103"
     CDS             4075..4929
                     /gene="aroE"
                     /locus_tag="IWM35_001103"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HAP9918969.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            5107..6270
                     /locus_tag="IWM35_001104"
     CDS             5107..6270
                     /locus_tag="IWM35_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HAP9918970.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGVQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            6387..6797
                     /locus_tag="IWM35_001105"
     CDS             6387..6797
                     /locus_tag="IWM35_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAP9918971.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            6971..9199
                     /gene="topB"
                     /locus_tag="IWM35_001106"
     CDS             6971..9199
                     /gene="topB"
                     /locus_tag="IWM35_001106"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAP9918972.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            9337..9654
                     /locus_tag="IWM35_001107"
     CDS             9337..9654
                     /locus_tag="IWM35_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9918973.1"
                     /translation="MKKVHSFLNYLMGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAY
                     SPTLILSTLTAGAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQ
                     YFG"
     gene            9814..10050
                     /locus_tag="IWM35_001108"
     CDS             9814..10050
                     /locus_tag="IWM35_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAP9918974.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
     gene            10047..10445
                     /locus_tag="IWM35_001109"
     CDS             10047..10445
                     /locus_tag="IWM35_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HAP9918975.1"
                     /translation="MRYLSTKEIIKINAKVILRHSPGEMIGVKDANALDMAVKQPSQA
                     VFNQELYPEIYEKAAILAINLAKKHPFHNGNKRTALVAMLLFLQLNNCPVAFSRQEAV
                     DFIIMITTSSLDFDSLKSKITSYLRQTAIK"
     gene            complement(11112..12056)
                     /locus_tag="IWM35_001110"
     CDS             complement(11112..12056)
                     /locus_tag="IWM35_001110"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HAP9918976.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
     gene            complement(12352..13296)
                     /locus_tag="IWM35_001111"
     CDS             complement(12352..13296)
                     /locus_tag="IWM35_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9918977.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLQIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            13517..14731
                     /locus_tag="IWM35_001112"
     CDS             13517..14731
                     /locus_tag="IWM35_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP9918978.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASICGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            14753..16768
                     /locus_tag="IWM35_001113"
     CDS             14753..16768
                     /locus_tag="IWM35_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HAP9918979.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEEAFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(16851..18224)
                     /locus_tag="IWM35_001114"
     CDS             complement(16851..18224)
                     /locus_tag="IWM35_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HAP9918980.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(18250..18534)
                     /locus_tag="IWM35_001115"
     CDS             complement(18250..18534)
                     /locus_tag="IWM35_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP9918981.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            complement(18536..20551)
                     /locus_tag="IWM35_001116"
     CDS             complement(18536..20551)
                     /locus_tag="IWM35_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAP9918982.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            complement(20730..21035)
                     /locus_tag="IWM35_001117"
     CDS             complement(20730..21035)
                     /locus_tag="IWM35_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HAP9918983.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            complement(21124..22155)
                     /locus_tag="IWM35_001118"
     CDS             complement(21124..22155)
                     /locus_tag="IWM35_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HAP9918984.1"
                     /translation="MLTIAYLGNGKSTNRYHLPFSRKLTNKINIKKIFSPSEPVWKTF
                     EDIEYTDQLSDLWDDPEIQLVVITTPSQFHYEYGKMALEHGKNVLVEKPFAETSKEAE
                     ELFALAKEKGLFIQCYQNRRFDSDFLTVQKVIESGKLGDILEVEMHYDYFRPEVPENT
                     HEFSTANSYLYGHACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMK
                     ISIKSSYFRIKERPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGV
                     LTYIDEQGSYHEEKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIK
                     SIQESEGKI"
     gene            22362..23237
                     /locus_tag="IWM35_001119"
     CDS             22362..23237
                     /locus_tag="IWM35_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP9918985.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            complement(23389..24231)
                     /locus_tag="IWM35_001120"
     CDS             complement(23389..24231)
                     /locus_tag="IWM35_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP9918986.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(24323..26200)
                     /locus_tag="IWM35_001121"
     CDS             complement(24323..26200)
                     /locus_tag="IWM35_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAP9918987.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVGNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            complement(26197..27618)
                     /locus_tag="IWM35_001122"
     CDS             complement(26197..27618)
                     /locus_tag="IWM35_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAP9918988.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            complement(27621..31010)
                     /locus_tag="IWM35_001123"
     CDS             complement(27621..31010)
                     /locus_tag="IWM35_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HAP9918989.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNTWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKDLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            31267..32658
                     /locus_tag="IWM35_001124"
     CDS             31267..32658
                     /locus_tag="IWM35_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP9918990.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEISEQIRKNM
                     SYCSFSSADCKEKNEEYVVQLLTQIYFFSRIFPYDTTHLTIRATYFAQQMQHRQIRLY
                     AGFRAELQFYSTLLNQNLIYYLSSYIFWMLIGNPEIHHYTSDKKILIISDLSLRHSQY
                     IEEYISDILAVHKIHSETTAITEDQLSKYNLLEYDLIVTNQPILNAHVPHILIDDSVS
                     FANEEELIRLFEL"
     gene            complement(32731..33378)
                     /locus_tag="IWM35_001125"
     CDS             complement(32731..33378)
                     /locus_tag="IWM35_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP9918991.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(33386..34072)
                     /locus_tag="IWM35_001126"
     CDS             complement(33386..34072)
                     /locus_tag="IWM35_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9918992.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(34078..35151)
                     /locus_tag="IWM35_001127"
     CDS             complement(34078..35151)
                     /locus_tag="IWM35_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HAP9918993.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            35373..36047
                     /locus_tag="IWM35_001128"
     CDS             35373..36047
                     /locus_tag="IWM35_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9918994.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            36052..37392
                     /locus_tag="IWM35_001129"
     CDS             36052..37392
                     /locus_tag="IWM35_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP9918995.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(37541..38527)
                     /locus_tag="IWM35_001130"
     CDS             complement(37541..38527)
                     /locus_tag="IWM35_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HAP9918996.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
     gene            39251..40282
                     /locus_tag="IWM35_001131"
     CDS             39251..40282
                     /locus_tag="IWM35_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP9918997.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
     gene            40391..41923
                     /locus_tag="IWM35_001132"
     CDS             40391..41923
                     /locus_tag="IWM35_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HAP9918998.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQIPILYLMEQPALSVSQSFLD
                     KNEISYMTHTSGTTGIPKLICHSAHSMGWRTKWQKTVFTKISEKKLIAFHISPVHSRF
                     NIGISSLMSMGFPMMPLANAQSSKVVHMLEAHRPIALETHPNNFVQWRFTAKEHPEAF
                     SGIRYYHSTFDAINNQTMKTFLRTSMAQDAVFLQVYGQSECGPMILKAHTLESLKTSD
                     ARDMGVGLEDMTSARITDSVGNVLPENTDGHIQLLSKGRALTYYKEDARFQENVYGDW
                     WDSGDYGFMDKHGHLFLKDRQVDLIETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSP
                     QPIIALAPDKEMDWNRWWEQVHELPRLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQ
                     KS"
     gene            42062..42874
                     /locus_tag="IWM35_001133"
     CDS             42062..42874
                     /locus_tag="IWM35_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HAP9918999.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            42864..43349
                     /locus_tag="IWM35_001134"
     CDS             42864..43349
                     /locus_tag="IWM35_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP9919000.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            43372..43917
                     /locus_tag="IWM35_001135"
     CDS             43372..43917
                     /locus_tag="IWM35_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HAP9919001.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(43990..>44677)
                     /locus_tag="IWM35_001136"
     CDS             complement(43990..>44677)
                     /locus_tag="IWM35_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HAP9919002.1"
                     /translation="VPRAVSVFGDTRPDFWAMSAKAIAMMYFFLQGTPFIYQGQEIGM
                     TNMPFESIDQVDAVDSKRLYKRLLAEGKTREEALDIIRETTRDNSRTPMQWTSEQYAG
                     FSTHEPWLITNPNTKTINVEQQEYEPESVLQFYKNMIRIRQTNKGLIYGSYKEYLHEH
                     PQLYVYERYLEDEQYLIMVNLTESLADYELPKEADQSWTLLLSNSSSGEFEAKGILAP
                     YEARLYKTNK"
BASE COUNT        13804 a         8111 c         8477 g        14285 t
ORIGIN      
        1 aggagaaaaa gtagaaaaga aggtgtttgt tatttgggaa gtagttaaat cgagagtaca
       61 agaaatgaat gaacatttct cagtgtcatt cgcagaaaat ccaagtaagt attttcgacg
      121 tcttgttttt ctgctcatct tatcattgct gttaacagtt gcaatgtttt tattgccgga
      181 aaaaatccga gaagagcaag taaatagtct cgtttattcg tccataccaa ccagcaaatt
      241 gaaacctttt gattacagca aagctgatca gaaaatcaaa caaagccggg cgatatcggt
      301 gatgttcagc aaaccgaacg ggaaaaccta tcaggacatg cttgatgttt ttaatgatcc
      361 tgagcttatg gaagaattga atcgaccgat cttttattat ccgatcgttt atgatgtaca
      421 tgaactggaa caaaaatata acatacgaac ggatgaagtg acatttattt tctttgatgg
      481 aggaaaagaa gcaaatcgga taactgcggg taagggagga ataactgatt ttgataagga
      541 gttgatccct gaactgaatc gacttccgtt agccaatatc aaacaattag aagaagaatt
      601 ggatagaacg ggagtaactt cccaaaattc cgagattgct aattgagaca ttggcgcgat
      661 gaaacggtgg gtctatatag cgctattttt aattatcaga acaagtgatg tttgatcttc
      721 aaaataacaa ataaaaaatt aaaaaatata gttgacgaaa ttcaaaaatt cgattagact
      781 taaatcaatt cttaaggaaa gggtgacctg taatgaactt atcaaaacgt atttcagcag
      841 aaaacaaaaa aaattggcgc agctggcgta gataagttgt attaatggtg agactttccc
      901 atagatacaa ctttttagag tgcatttcta aagcttgtat ctatgctaga catgaacatt
      961 gactgccgca tagattagaa tctatgcggt tttttgtgta aagacaatga ccgctttggc
     1021 cattgtcttt ttttgtagac tggatacttg cctgattata ataaataaaa agattgactt
     1081 tgcaggagga aaaaaatgaa aaacaaacga ttaataacag ttgtagtaat tattttagtt
     1141 tatttagcag gtgcatttat ttatggaaaa gcctcaggcg aaaaagaaag tacttcgacg
     1201 aaccagacaa aaaaaacaac agttgttggg gtattgcaat acgtgagcca tccggcatta
     1261 gatgagattt atcgaggaat caaagatggt ctgaaagaag aaggattaaa agaaggagaa
     1321 aatcttgaga tcaaatttca aaatggacaa gccgatcaga gcaagctagc cacaatgagt
     1381 caacagctag tacaatctga tccagatgcc ttgattggaa tcgcaacacc tgccgctcaa
     1441 tcattagcaa acacgacttc atcgatccca gttgtgttag gtgctgtaac ggatcctgtg
     1501 ggggctggat tagtcgcttc tttagagaaa cctggtggta atgtaacagg tgtttctgat
     1561 cagccgcctg ttacttcaca aatcaagctt gcttctgatt tgttgccaaa tgcaaaaaaa
     1621 gtcggaatct tgtatgcttc ttcagaggat aattcaaaat accaagtagc acaagcagaa
     1681 gaagctgcaa aaaaagaagg gttagaatca gtaaaatatg cggtgccttc cactaatgag
     1741 attgcacaaa cggttcaagt gatgagccga caagtcgatt ttatctatgt tccattagat
     1801 aataccattg ccaatgcgat gcaaacagtg gtaaaagaag caaacaaagc aaatatcccc
     1861 gtcattccat ctgtggatac aatggtggaa caaggtggac tagcaacaat cgggatcaat
     1921 caatatcaat tagggcttca aagcggtaag atggcggcag aacttgcgag tggaaaagaa
     1981 aaaccggaaa cgacgcctgt ttatacattt gatcaagggg atacagtgat caatcaatct
     2041 caagctgatc gcttaggaat cattgttccc caatcgatga aggaaaaagc caaaatcatt
     2101 acagatgact cgcagcaaga aacatctaag gaggatgaca aatgatcgta tcagctattt
     2161 cacaaggcat gctttgggca atattaggat tagggatttt catgacttat cggatcttag
     2221 actttcctga tatgacgaca gaagggtcct tcccattagg tgctgctgtc tgtgtgacag
     2281 ctattactca tggggtagcg ccgattgtag ctacattatt aggcgtaggt gctggcatga
     2341 tagcaggact cgttacgggg ttactttata caaaaggaaa gataccggtt attttagcag
     2401 ggattttagt gatgtcagga ttgaactcag ttattttgtt tgtgatgcga acaccaaatc
     2461 tttctttatt gaatcatccg attctacaag acagcttcca tcgctttggg ttacctgatt
     2521 actttgacac agtccttctt ggcttcatct ctttgggcat cttgattgct gtactgctat
     2581 tcttcttcaa tacagatttg ggtcaaggct atattgcgac aggagacaac gaaacgatgg
     2641 ctcggtcgtt agggataaaa accgatcgta tgaaaatttt aggtttaact ttgtcgaacg
     2701 gtgtcattgc attatccggt gcgctgattg cccagaacga cggatatgca gatgtaaata
     2761 aagggattgg cgtaatcgtc atcggtcttg cttccatcat catcggtgaa gtgatcttcc
     2821 aagaattgac gctagcagaa agattgattg ctattgttgt cggcagtatc atttatcagc
     2881 tattgatttt agtagtaatc aaactagggt ttgacacgac ttatctgaag ttgttctccg
     2941 ctatcatctt agctatttgt ctgatgatcc cgcaattgaa gaaagcactg aacttaaaaa
     3001 cgggttttga gaaggaggca tagtaatgac tgatatattg acaatcaaaa atggcagaaa
     3061 agtcgtaaac aatggaatga atgaagagaa agtactattg gatgaaatta accttacaat
     3121 cagagaaggg gacttcatca ctgtccttgg cgggaatggt gctggaaaaa gcacactgtt
     3181 caatacgatc gctggtacgc ttcaactcag tcaaggatcg atctctttta aagatcagct
     3241 gatcacaaaa gattcagaag aaaaaagagc acgattcctt tctcgagttt tccaagaccc
     3301 caagatggga acggctcctc ggatgacagt agcagaaaat ttgctacttg cacaaaaaag
     3361 aggtcaaaaa cggaaattcc gtttacgtaa cttgaaaaat caaaaagaag attttttcag
     3421 actatgtcaa gaagtcggta atggcctaga gcaacacttg gatactccag cgggtgaatt
     3481 atctggtggt caacgccaag cattaagctt gctgatggcg acgatcacag ctcctgagtt
     3541 actattatta gatgaacata ctgcagcttt agatcctaaa acagctaaag cattaatgcg
     3601 attaacaaat cagcggatca atgaacaaaa attgacttgt ctcatgatca cgcatcgaat
     3661 ggaagacgcc ttgaaatacg gaaatcgtct gatcgtactc caaaaaggga aaatcgttca
     3721 agatatttct gaaaaagaaa agcaacattt gacaatgggc gaccttttgc agttctttga
     3781 agaagtagtg gattgatttc gacagaaaaa ttcctttcag tagaaggact tgaaaactca
     3841 ttgacatctt cctttgattc atcctatact taaaacaata aacaattaaa catatttggg
     3901 tgttttgtag gatcacgtag tcaatgattt taacaattaa atagaatatt ctttgctctg
     3961 acaagtaaac gatgagaaaa gaatttgcta ctttgccacc tagacgtttc gtactttttc
     4021 gtatcaaacg ctaagtggct ttttttattg aagtaagaac aaaggaggaa taagatgatt
     4081 tctggacaaa ccaaattagc tgggctcttt gctgatccgg cttctcacag tttatcccca
     4141 ttgatgcaca atactgcttt tgaagcaaac aacatcgatg cggtgtattt agctttccaa
     4201 gtaaatccaa tgaatttgaa gcaagcagtc gaaagtattc gaacttttga tatgttaggt
     4261 gtaaatcttt cgatgccgaa taaaacagcg gttattcctt atctggatga ggtaagccag
     4321 gaagcgcaat tgatcggagc agtgaatacg atcgtgcacg aaaagggtcg tttgattggc
     4381 tataataccg atggtgcggg cttcatgcaa tccgtgcaag aagctgggat ttcgatcaaa
     4441 aagaaaaaaa tgactctact gggagctgga ggtgcagcaa aggcagtcac tgttcaagct
     4501 gcgttagacg gcgctgagga aatcgtagtt tataaacgaa acaaccaaac attcgctcaa
     4561 gtaaaagaaa cattcgaaca actcagctat cgaacttctt gtaagataaa agttcgtgac
     4621 tttgccgata cgcatcaatt aagaaaagac ttgaaagaaa gctgtcttct agtcaatgca
     4681 acagatatcg ggatgggaaa gaaaatcaat cagtcaccgc tcttagatat aagcatgatg
     4741 catgagaatc tagcggtttt tgatttgatt tatgcaccta gagaaacacg tttattaaaa
     4801 gaagcaaaaa aagtaggagc aaaaggatac aatggacttg gcatgttgat ctaccaagga
     4861 gcaatcgcgt ttgaattatg gacaaaggaa aagatgccga tccatgtgat tgaaaagctg
     4921 tttgattaaa aaaggataat agataaagat cttttattct cgcataaacg gtctcaagaa
     4981 aagtaatcct ttatgtatcg aagcatttat ttcgttattt tttattaaat tatcaaaaaa
     5041 tgataggctt ttgtcgattg gttatcgtat cctttattta tcagtaagaa acgaggagga
     5101 accacaatga gtcggatgga tcgctataag gacatccata agaaggcgaa acctctgaag
     5161 cgggatacgg ggagtatttt tgctagaaga gaacgaaaac gaacgcgtat ggaaaatgag
     5221 gaagatgcta cccgtattta tgaaagagag cagcattcat ttgaacattt gaaccaatac
     5281 gagcaacaga tatacagaga tgcaccaaga gcagaagaac aacaaaaaaa taaaaaattt
     5341 ttcaatcggc aaaagaagaa caaaccgtta aaacctcgaa gaaagcgatc gtggaaaaaa
     5401 atcattctat cgattttgct tgttttgatc ctttattcag gtatctcatt ttttattggt
     5461 aaatatgtag ctgaacatga cacaagctta gaggcagcga cttcagaaac ctttcatggg
     5521 caaaaagcga cgaatggtgc gcataatatt ttgatccttg gaagtgatac acgcggagaa
     5581 gatgccgggc gagccgatac gatcatggtc ttacagttgg atggtccttc ccataagccg
     5641 aaactgattt cttttatgcg tgacagcttc gttacgatcc caggggtagg acaaaataag
     5701 atcaattcag cttatgcata cggaggagcc gatctagtgc gtcaaacttt agtagaaaat
     5761 tttggaatcg attgtcaata ctatgcgaaa gtcgatttca aatcgtttga aaaagtgatc
     5821 gatgccttat tcatgaacgg agtgaagatc gatgcagaaa aagatctgaa tctggatggt
     5881 gtagatatta aaaaaggggt ccagaagatg gatggacatg ttcttttgca gtacgcacgt
     5941 ttccgaatgg atgaacaagg agactttggc cgtgtcagac gccaacagca agtaatgaac
     6001 gctattttca gccaattgaa gaatccattg aatctgatcc gtgcacctta tgcagctggt
     6061 aaagctttag gatatacatc cacagatgtc actgcttggt tcttgacgaa aaacctcttt
     6121 tcgattgcac gaggagctgg tggcattgat cgaataagtg tgccagtaga cggttcgtgg
     6181 aattatgggc aaagtgatta tgctggcagt attcttgaaa ttgataatca aatgaatcaa
     6241 tctgctatta cagatttttt gaataaatga tcataaaaaa gatcaagaag aagctgcgca
     6301 aggcttttgt catgaatcaa tcaaacgatt cactcgtaaa aaacgctttc ttatggtaga
     6361 tttaaccaag agaggagctg tttacattgg atatctatct gaacttcaaa aatgagtcgc
     6421 gtgaagccat cgctttttat gaaaaagttt ttcagactga agcatcaggt atcaccacat
     6481 acggtgaaat gcctggaata gaacaaatga acttgccaga agaagcaaaa aatctaatca
     6541 cgaatgcacg gctgaacatt tatgatacat tagttatgtt tgcagatacg ccagaaggaa
     6601 ttggtcctga attagttgtg ggaaataatg tttctttagt catccagaca agaaatgaag
     6661 aagaaacgaa gcgcctattt gaaagcttag ctgagggtgg taagattgag atgccactgg
     6721 atgctgttcc gtgggctcag ttgtatggga tggtaactga tcgatttggc atcaactggc
     6781 agattaacca agagtgacag ctaaagatga atattttctg gaaacatcaa gcagcaaacg
     6841 gatcatcgtt tgtttgcttg atgtttttgt gtctatttcg actatacggt atcttttttt
     6901 tattgccaga tgcgaaaata tgttccataa tagagaagtc cgcaaaaaat taagtaacgg
     6961 aggtcagaag atgaagacgg tgatactagc tgaaaaacct tcacaagcaa aagcctatgc
     7021 cgaaaccttc caacagcaaa agcggcatga agggtattat gaaatcagcg atccattgtt
     7081 tgctggtgaa gtaaccatca cttatggttt tggacatttg gtagatatgg ttgctccagg
     7141 agcttatgaa gagcgttggg caaaatggtc tttagaaaat ttgcctattt tcccagaaac
     7201 cttccgttac gaagttccaa tggacaaacg tgcacaattt tcgatcgtaa aacgagaatt
     7261 gcagtcagcc gataccatca ttattgcaac agatggtgat cgagaaggtg aagccatcgc
     7321 gtggtcgatt attcgacaag caaacgcatt ttctaaggaa aagaactatt tacgtttatg
     7381 gatcaattct cttgaaaaag aagcaatcta tgaaggtttt aagaatctcc aacctggaaa
     7441 gaactatttt ccaaaatata aagaagcgca agcaaggcag aacgctgatt ggctgattgg
     7501 aatgaatgga agtccattat atagcttgct acttcaacga aaagggattg atggcagttt
     7561 ttctcttggt cgtgtacaaa caccgacact ctatatgatt tatcagctgc aagaagaaat
     7621 caaacacttc aaaaaagaaa cgtactttga aggagaaggt ttaattacga caaacaaagg
     7681 gcagttttct ggaaaaatcg taccaaaaaa agcatttaaa acccaagaag agttaacgaa
     7741 ggaaatagaa aaacttggag cgcatttagg taagcaaaac ggacggattc ttgaagttac
     7801 gaaaaaggaa aagaggttgc atagtccacg tttattttcg ctatctagtt tgcagacaaa
     7861 gatgaatcag ctaatgaaag caagtgccaa agctacactt gatgctgctc aaggattgta
     7921 cgaaagcaag tttctaagct atccaagaac ggattccttt tacattacag aaaatgagca
     7981 tcagtatctg gttaaacatc tgcaaaatta taaaaaattt ttaggaatag aagaagtaca
     8041 gacaactgag atcaaagcaa agaaacgata tgtcgatgcg aaaaaagttc aagaacatca
     8101 tgcaattatt ccgacaaaga cgattccgac tcctgcacgc tttgcgaagc tttccaaatt
     8161 acagcaagca atctatttgc aagttttgcg cactactgtg gctatgtttg ctgcagatta
     8221 tatctacgaa gagacgaccg taatgacggg cgtacagcaa ttaaagctaa aaagcatcgg
     8281 aaagatccca ttacagcaag gttggcagga aatcttacgc atcaagtcaa aaaagaaaga
     8341 agctcagact cttcctccta tcgtagaaaa tgaagaagca gaagtagaac tgaaaagtat
     8401 ggagaaagaa acacaacctc caaaacccta taccgaagga acgttgatca cagcaatgaa
     8461 aactgcggga aaaacgctgg ataatgatga agcgcaagaa attctcaaag aagtagaagg
     8521 aatagggaca gaagctacac gagccaatat cattgaaaat cttaagcaga agcattacat
     8581 cgaagtcaat aaaaacgaaa taactgttac gccaaaagga atcactcttt gtaaggcagt
     8641 agccaatgaa ccattactga cgagcgcaga aatgactgcg cgctgggaag ggtacttgag
     8701 aaaaattggt aaacaagagg ggacaccatc tgttttttta gagaatatca aaaaattcat
     8761 cctacattta ttggcagaag ttcctaatca aatcgacaag gtagactttt cagatgagat
     8821 tagcgggaca cgtcagataa aagcgatgga gaagaaaaac gatcaattag gaagatgtcc
     8881 aaaatgtaaa aagggaatag tcatgcttta tcctaaaatc gctacatgct tgaatccgga
     8941 atgcgacttc aaattgtggc caacagtagc taaaaagaag ctgactaaaa cgaatctcag
     9001 agatttgctt tcaaaaggaa agacaagtaa agttgtcaaa ggttttactg gtaaaaaagg
     9061 gaaattcgat gcagttttag tattaaaaga agatatgaca atcggttttt cttttccatg
     9121 gatcgaaaac agtggaacaa aagcagttga tggaaacaaa gaaacatcaa gaaaaaataa
     9181 tgaaaacgaa gtagagtaaa tgaaaacaat gaaatagcgg aatatttatt ccgaaaagtg
     9241 agaatataaa taccaataaa actaaaaaat atcagtgaat caagcaaaaa cgttggggta
     9301 ggctgatttt atatagatag caaggagttt ttttatttga aaaaagtgca ttcattttta
     9361 aattatctta tgggagcaat cacgattgtg gctgtgtacc aagcatttct aaagtatcct
     9421 gattacggga cgaagttgtt gtcattgacg cacaatcaaa cagcatattc accaacgttg
     9481 attttatcga ctttgactgc aggagctaaa agcgctactt atttttttca taaaacgaca
     9541 agcatgctaa agcgaaagaa acagcgaaaa caattgttgg tggtggtagc ggctatgatc
     9601 attatttatt ttctaccagt tattacacaa gtatttcatc aatattttgg ttaagaatac
     9661 gagagtttgt gacaaaagtc atgtcacaaa cttttttctt tgtcattttt tcgtaagaaa
     9721 aagcaaaata aaagtatata cattgattaa aagtatatac ttggatataa tgtacttact
     9781 atagaatgag aatggtatag agggtggcga aatatgaaaa aaatgaaaaa gtataaaatt
     9841 agaaaatctg gcaatagcga tgtaacaact atcccaccag aagtaaaaga attcatgggt
     9901 gtgcagacag gtgacgcaat cagttatgtt tttcaatctg atggttctgt acgaatgatc
     9961 aaagcacaag aagaacctga tattgattca ttagttgatt cgatcatgaa tcaatatgaa
    10021 gacgcgttaa aagatttggt tgatttatga ggtatctttc gactaaagaa attatcaaaa
    10081 tcaacgcgaa agttatttta cgtcactcgc caggcgaaat gatcggtgta aaagatgcaa
    10141 atgcattaga tatggccgtc aaacagcctt cacaagcagt attcaatcaa gaactctatc
    10201 ctgaaatcta tgaaaaagcg gcgattttag caattaattt agctaaaaaa catccttttc
    10261 ataatggcaa taaacgaact gctcttgttg ccatgttgtt gtttcttcaa ttgaataatt
    10321 gtccagttgc tttttctcgt caagaagctg ttgattttat cataatgatt accactagtt
    10381 cattggattt cgattcacta aagtccaaga taaccagtta tttacgacaa accgcaataa
    10441 aatgaacgaa ttaaaaactt tatttctcaa ttagatcagc atgtcattaa ggaatatgct
    10501 gattttttta tgtttaatca tacaaaatag ttttttcata aaaatatgga aaatatggat
    10561 taattttgaa ataattgatt taaaaggaat acaaaagagt gaatagtagg aaatagtggt
    10621 ttagaactag tgggctgatt ttttatatat tttagtttac ataatatata ttatacaaag
    10681 tagatttaaa aaatgataaa cctctctcga acggtttttt atcaaaaatc gtcatttttc
    10741 catcaaaatc tgcttatgca aaattataaa aaaattttgc agtttttctt actttttttg
    10801 tcgtttaatt gaatgataaa aaccagtttc tttattcagt taatcaaata tgaacgaatt
    10861 agatatgaat cattgtattt gccgttgcgg aaggattttt taattaacca cagataaaca
    10921 ttgataacga ttcgcttatt gtaatcaaca gattaaattt catatgttca tatgtacgga
    10981 tacattttcg gaccgattaa ttttaaccat tctgtttatt tgcttaccta ttagatatca
    11041 ataaaaaact gcttaacagc tatttcagct gttaagcagt tttaggattt ttagttcata
    11101 ttcattgttt cttataatac agtgttcaat acttctctta aagcacttgt tgatttgctg
    11161 aacattgctt tttcttcttc gttgattgat aattcaacaa cttcacgaac accattttcg
    11221 tcaacgattg atggcacacc tgtaaagata tctttttcgt cgtattcacc agttaaataa
    11281 gcagatacag gcaatacagc ttgttcgtta ttcaagatag cttttacgat acgtgcagta
    11341 ctcataccaa taccatagta agttgctttt ttacgatcaa tgatctcata agcagcatta
    11401 cggactttat cagcaatcac atctaattca tcttgggcaa tacgatggtc tttttctaca
    11461 atttcaaaca ctggtttacc accgacagtt gtatgtgacc atgcagcaac ttctgaatca
    11521 ccatgttcac ctaagatata gccgtggacg ctgcgtgggt caacttttaa tttcaatgcg
    11581 atttctttac ggaaacgagt tgtatccaat gttgttccag taccgataac acgtgatgtt
    11641 ggcagaccag attcgttcca agcgatatat gtcaaaatat ctactgggtt tgaagcaaca
    11701 acgataatac cgtcgaatcc tgatcccatg atttctttta cgatttggcg catgattgac
    11761 gcatttgttt taactaaatc taaacgagat tgaccaggtt tttggttgat accagctgtg
    11821 ataacaacga tatcagcatc tttacattct tcatagccac ctgaccatac agccacattt
    11881 tcatcgcccc aagccatacc atctaaaagg tccaatgctt ccccttctgc tttttcttga
    11941 ttcacatcga tcaatactaa ttcattagag attccttggt tgatcattgc ataagcgata
    12001 cttgttccaa caaaacctgt accgacgatt actactttac gacttgtttt tttcatgaat
    12061 aaacagctcc ttttcataac tatttgttac tttcatgaaa agtatagtct tgtttattca
    12121 ttttatcaac atgttaatta taatagttgt tattataatt tttgatggaa acgaattcct
    12181 gaatacgcat aacagataaa tgacaaatgt ttacacctat atcccctgat aatacaacca
    12241 aaagtttaga actgaaaata aatccaaaaa aattcgctgc aacgatataa taagacagca
    12301 atcgcaccta aaacaatttt ccttcatatg ctttagtgaa agattttctt cttaatgttt
    12361 atttctccat tcattgggag tacatgttga atacttcttg aataatttga ataaagaagt
    12421 cgtttgttta tatccgatac gattagcgat ttcttccaac gaaaaactcg tttcttgaat
    12481 cagttccaac gcaaagtcga attttttctt tagaaccagt tcttgaaagg aagcatcaaa
    12541 atacattttc aatttattac taagatagac cggattgtag ccaaaatgag ctgctgtttc
    12601 ttttaacgta caagaagtaa aatgcttatc gatataatct aatactctaa gcatctcatt
    12661 ttctgcgcat ccatctgctg atgaaaagct ttgttgttct aataaatgag tcaattcgat
    12721 cagtaatagt gatgtataga gatttaacga acggatctta tgactgtatg tatcaaaata
    12781 aatcaacagc atattttcaa tcaaatgcgt caaacgactt gtttttccgc cgcgaaaatg
    12841 aatgaattgt gtatgttccc ctttttcttt tgatgcattt atcaaaaaag aagaaagtag
    12901 attatttgtg cttgccaaat gattcaatat ctctgtagat aaggtttctt cttttaataa
    12961 gatgttgatt aaaatatcct cttgccctgt gtattcgatt ctttgagtag cttctttatc
    13021 aatgatcacc acgtcacttt cttccatctc tatcctttct ccattaataa tttgtaaaca
    13081 tgtaccgcta tacatataat tgatttcaac aaaatcatgt gtatgctcgg gaacttccgc
    13141 atatcgacgg tgtttactga tatgaatatt tccctgttga aatagatccc ctcggtggat
    13201 ttttggcggt gtctgaagat ggtaagctaa gtttaaatca ttgatattca ttccttgttc
    13261 acgctgcaga gtttcgattg attcttcgtt acgcaaaaaa aatgtctaat tcagctagtt
    13321 ccaattgact ccctcctttt tgtaagcggt ttctttcgct tattagttca cgctgtactg
    13381 ctattatatg caaagctatt tttggtttca taaagacttt ctatggttgg gacatgaact
    13441 tcctgtgatt ccgctattga gagaataacc acttcatact atatttttga taagcgctta
    13501 caaaaatgaa aggaacatgc ttatgaataa aaagatgatg aaattttctc tgctgtcagt
    13561 ctctcttatt ttgacttctg ctggtgcagt cacgtctgca attcccagta tgtcagctac
    13621 ttatgccacg cgttctttat ctgctgttga attattaacg actgctccct cttttatggt
    13681 cactattttt gttttgatta gtagttttat tgctaggaaa atcggacaaa aacaaactgt
    13741 gatcatcgga ttagtgattg catcgatttg tggtatcctc cccctcttct cacaaagtta
    13801 cagtattgtc ttagcttcaa gagctggact aggtattggc tttggattga tcaattcttt
    13861 ggcagtcagc atgattgctg ccttttatca cggagatgaa cgagcctcta tgattggttt
    13921 tcaaagtgca tttcaaggat ttggtgcagc tttaatgaca tttgttgcag gacagctagt
    13981 acagttcggt tggcaatata cctttttagt ctacgccatc agcttaccta ttcttgtctt
    14041 gtttgttttg tttgttccta cacctgaaac aactgaactt caatcgcatg gcacagcccc
    14101 aacaagacaa gtaatcaaaa cgaatcctaa aatgattagt tatggtattt ttctcttgct
    14161 ggtcatcgtg gtatataatg ctgtatcgat taaattagcc acagtcctga cgacttattc
    14221 tctaggaaac gaaactaatg cagcgaatat cttaacaatc atgcaattgg caagtatggc
    14281 tgccggagtt tgttttggct ggcttcagac aaaagcacat cgctatatgc ttacactttc
    14341 tttactactg atgggaatcg gctttgtatt gattgccact gtgataaacc tgtatgtgat
    14401 tgggtttggt gcgatcctaa caggaatttc tttttcgtta ttcattcctt acttattcaa
    14461 ccaagtaagt atccaagcgc ccaagtacgc ggagagtttc aatacttcaa tcttattggt
    14521 tggcgctaat ttaggtactt tcattgcacc atatggtttg ctctttctct cttttgcttc
    14581 tcttgggagt aaaacacttg ctgttttcgt caatggcagt atcttgctgt tgattagcgc
    14641 gcttattagc cttctagtag tacacaggac gaatagaaag acagtgataa gagtagatga
    14701 agaaataaaa gaacaattga caacaaaata aatttgatgg aggtcgtgta aaatgacaaa
    14761 tgaaaaacat aaagatagat ggctttggta tcctggagac tttgagatcc gccatggact
    14821 gctacaaaat ttccaacggg aagaacgagg ttttgactgg ccagcgtact ggtacatgga
    14881 tgattgccat cggaacgtca agtttaagag atattacttt cttgatcaac cttccatgtt
    14941 taaagtgacg atccaaggag ttgggtatgt agaaattaat ggacaaaaac atccatgtgg
    15001 aaaatggctg acttgtccag ctggtaaagc aaaaattcgt atctttgtag gacatacatc
    15061 agggctacca gcgatgttta tcgagggaga cgaagtaaaa tcagatattg gctggacagc
    15121 aagtaatttt attgaagaat atccagctgg ctggagtccg ctttatgtcg atatcgcaaa
    15181 agatccaaat caaatctatt atcaaaaaga atgcgtccat ccagtcactg aaacggagat
    15241 tggcggagga atcttattcg attttgggcg agcagtgaat ggctgtgtag aactaatatc
    15301 tctggatgac catatcgaat ctatcactct atgttatgga gagtccgatg tagaagctat
    15361 ggatatcgat tattgctact ataagcaaat aggaatgaca agaaaaatga aagctcgtaa
    15421 acgcgctttt cgctatttgt ttataccaga ggtttctact ggaatggttc aacttcaagt
    15481 ttttcatgaa tttttatctc tttcgaaaca agctgctttt tcaagcgatt cttctttact
    15541 gaatcagatc tggtcagtat cagaagaagc cttcaagtta tgtagtgatt tgttttttat
    15601 cgacggtatc aaacgagatc gctggatttg gtctggagat gcgtatcaat cattcatgat
    15661 caatcaatat cttttttttg acgaggaaat caataaacga acgatattag cattaagagg
    15721 acaaaatgaa atcaagcagc atatgaatac tatcgttgac tattcgatct tgtggttgat
    15781 tggtatcgaa aaccactata tgatgacgaa agactatgaa tttttacgca tcgtctaccc
    15841 taagatgcaa tcattaatga gatacttgat ggagcaaacg aatgaattag ggtttattta
    15901 tgggagagca aaagattgga tatttattga ttggtctgaa atggataaag aaggaacgct
    15961 tgctgcagaa caagtcttat tactgaaagc ctatcattcc atgatcactt gcggagatat
    16021 cttagctgaa acatatggag agaacgaaaa ttatcatact catctctatc aagaaaaata
    16081 tcaactattg aaacaaaacc tatttgacta cttttgggat gaagaacttg gcgcttttat
    16141 cgattgctat gaatctggga gaagaaatgt gacgcgtcat gcgaatattt tcgctgtttt
    16201 attcgatttt gctaacaaag accaacagca atcgatctta aagaatgtct tattgaatga
    16261 cactatcaca cagattacta ctccatattt caaattctat gaacaagatg ctttatgcaa
    16321 actcggacag acgaatatcg tctaccaaac gattcttgat tattggggag ggatgttatc
    16381 tcatgatgcg gtaactttct gggaagaata cgaccctact caaacaggaa atgaaaagta
    16441 cagtatgtat ggagatcctt atggaaaaag cttatgccat gcatggggag caagcccaat
    16501 ctatctttta ggacgctatt ttatcggttt gtatccaaca aagccaggtt atgaagaatt
    16561 tatgatacaa cctaatctag attcatttga aacactgaat tgtacgctac ctatcaaaga
    16621 tggaaagatt tcgcttgaaa aaacaaaaga taagatcgtg attacttctg atagaacagg
    16681 cggaacagtg aaaatgaatg gtaaaagtta tcctcttcta gcaaaacagc cacttgtctt
    16741 aacaagctat tcaaaaataa atgcataaaa caaaaaactg cttgcgacat atcagaagac
    16801 tttaaatctt cttctgatat tgtccaagca atttttattt tgaagtttgc ttaattctgt
    16861 tctttagaaa caatgtttgt tctttttccg gcaaaaagaa agctagataa tacgacaagt
    16921 aaaaagaaag cgatgattcc aactgtcaag gctatttgac cgtattctcc tagtttccca
    16981 agtacaagtc ctggtactag ataatcagta tcggcaaatg tagaagaagc gattttaaga
    17041 tctcctaata ctggaaggat aaataatggc agccaactaa tcaataagct attgaaagca
    17101 gaacctaata ccgcccctct tctaccgcca gtcgagttgc cgaagacacc agaagcagct
    17161 ccgcagaaga aatgtcctac taccccagga atgataactg tagtttgcaa gcctatcata
    17221 gcaaacatgg atagtgttcc tacaaagaag ctaacaaaaa agccaattaa tacagcatta
    17281 ggtgcatatg gaaaaacgat aggaacatct aatgctggtt tagaatcggg tacgagtttt
    17341 ttagcaatcc cttggaatgc tggaacgatt tctgccaaca ccattcgaac cccttgaagg
    17401 acgataacaa acccagctgt gaatgttcca gcttggatca gtgcaaagat tacagcgttg
    17461 tctccattgc ttagttctct ttcaacaaat ccaggaccag ctaacaaagc aagtgcgaca
    17521 tatacgatcg acatcaagag catgatcgat acagtagaat ctcgcaaaaa gctgaaactt
    17581 ttaggaatct ttatgtcctc tgtagaacgg tctttgtttc caaaaaactt tccgatccat
    17641 ccactagaag cataaccgat atttccggta tgccctaacg caacttgatt gtttccagtg
    17701 atgttcttca taaacggttg acaaagagca ggcgtaatag ttaatagtgt tccttcgaag
    17761 accccgccta ataagatcat ccacaaattt gcagtaaacc ccgcgcttac taaaatgact
    17821 gctgttagac aagaaacata aagcattgct tgacctgtaa gaaaaatgaa tttatatctt
    17881 gtgaatctag caagtaaaat attcacgatc atccctacta gcatgatcaa agcagtaggt
    17941 gtaccgtatt gttgcaaagc aatcgccact actgcttcat tgctaggaac tactccttga
    18001 gtatgcagag ctgtttggaa catcgaagca aaaggggtta gtgaattact gattaaacca
    18061 gcaccgccga tcagtactag gaatccgaca aatgttttga tagttccttg gatcacttct
    18121 gaaaatttct tcttctgaag aacgagacca agcagtgcaa tcaaagaaac gaggatggag
    18181 ggctgactga cgattgacac aaacaaattc aacaatttat ccattgatag ttcctccctt
    18241 gaatcatttt tattcttttg acaagatctt cgttactttt tcttttactt cttcgctgtc
    18301 aatgatcgat tgcaaaggta cgagcttttc atcaggtata gaagttagcg tatctttcaa
    18361 agaatgctca atgaagaaat aatcagccat atcgctagat acacttccaa catccgtatg
    18421 acttacttca aactcgtcca catctgctcc aagatctgca agtgcttgtt tgatgttcat
    18481 ctctaccata aaacttgttc ctaatccact ttgacatact gcaaaaattt tcatatcatt
    18541 cgtctccttt tcttatgatc gctaatattt cttcttttgt tgatgcgtgt aataaatctt
    18601 gaagattttg tttattagaa agtagctttg ttaaatttgc caaagcccga agatgtgcct
    18661 cattatcgat agcagctaaa cagatgaaca aactaatggg atgggctgga ttatcaagca
    18721 gatccacagg ttgttgaagt ttcagcaaag acatccctac ttcgtttacg ccatcttctg
    18781 gacgagcatg aggtaatgcg atgtgatcgc caatatggat aaacgcacca tactgctcca
    18841 cgcgctcgat catagctttg atataacttt cttctatttt attttctgat aatagcggct
    18901 gtgcggcaaa tgtgattgct tcttgccagt tttttggttg atccgataat tggatcattt
    18961 cttctgttaa taattcagat agcataggac gttgatcctc cttcttttct aaaagtttgt
    19021 tcattttacg atttaatact tgatagagct tttcttcgtc tacgccttct tttaattcga
    19081 tataaggcag tagcgccgac aacaattctt gggcagaagg cattgaaaat cctggtatca
    19141 gccaatctct ttgtacttga ttgatcagct gatttttttc caagtcagtc atgattggat
    19201 gcagaacata gagagacttt ttcttatcag taagtgtgag aggtacagta gaaaaaataa
    19261 tatcgtaaga agtttcgtca acctcgtgta agcggtcagc tgaattagtc tctgtaaaaa
    19321 agatcatcgg gaataattgc tgaagttcac tttttaagat caatgaagag ctaatgccac
    19381 ttggacagac gatcacggct cttatttttc tatttctttc ttctgcgtcc gcttttctga
    19441 tctctccgcc aaatagtata gtgaaaaaac caatctcttc ttcagggatg ctcttcccta
    19501 ttcgtctttc taacggaagt aatgcttttc ttgtcatttc gtagatggaa gcatattgat
    19561 gcttaatttg atcgatcatc acattaggta aataaaaaga ataggaaata cgaaaatagg
    19621 cagggaccaa gtgataaaag acattcatca ataattcacg aaacgaagca aattctacgg
    19681 ccatcaggaa ttccatgcga tggatgactt ctgaagaaca ttccagcaaa aaatcaagtg
    19741 caggctcctg gacatctcct tcaacgatcg atgctaagca aagagagaaa aatataatct
    19801 ccgaattttg atgttcggaa tagccaaggc cagtcaatac ttgtcgacta atgtcagtga
    19861 ctgcaagaga agccaacagt tcagcatctt ttaccagttc catggatgtt tttccaattc
    19921 gaatggttgt caaactcaaa taataagcta attcatccat tcgatttggt gcgaatcgaa
    19981 gatctgatag tgaaatcgtt ttatctagca aatctctaac tttggcgtaa taatcaatgg
    20041 aaagggttgc ggttaaatac gtgagcccaa atcttccagc aagtgactgc atcaaacgag
    20101 cagtgcgatt ttttgcttct cttcttaata ttgcttctga ccctagcagc ctaaaaccag
    20161 ttttcctagt atacgacagc tgacaatctt ccacttctaa ttcatttctt atctttttga
    20221 tatctgacag gactgtccct ttagaaacct gaagaaaatc ttgaaaatga aaaacagaaa
    20281 cagacgttgc agtcgaataa accatcaaat aaagaagatc tcttcgctct tgttcggtat
    20341 acacagcttc aaatcgagta atatccaaaa atttttctgc ccagtcgatt gctcggaatt
    20401 tctttatacg gatacgggta tcatccacat attcggcaaa aggagttaat tgtttgatcg
    20461 cctcgcttgt atttacttcg atgatctctg ctgctttttc tagactgatt tctcttggtg
    20521 ttttcaggag ataactaatt ttgtagttca cagcgctgtt cacctcctac ttatattcta
    20581 gtatggtagc gcttttaaat cgaggggtgt ttggtacaac ttacagttgg atttttttta
    20641 cgactgtacc gaacgatttc tatcgaatgg atacagaaaa aaagcaggtg accaaatgtc
    20701 tcctgctttt atgaattgat ttcgtccgat caatgatgat catgtttatt ttttgaggtc
    20761 agttcttcaa atttttcttt gacatcttca accgcttttt cgatatcttt ttcaaagtga
    20821 ttgggctcat taggctttgt ttcctcttct acagaatttg aaccagataa agctgtttct
    20881 ttattttctg tttcaccaga gtttgtgcca gctaatgatt cttctttgtc ttctgtttca
    20941 ccagaattag ttcctgctaa agccacttct tcttctgttt taggggccat tgaacctctt
    21001 aatcgtttgt gtttttcacc gtcataattt gtcatgttgg ttcctcctag tagttctata
    21061 ctttttcatc gtacaataag acagtttatg cgtcaaaaaa caaacccgat tcttacaaat
    21121 atgtcatatt tttccttctg attcttgaat gcttttgatt ccttcttcca aaattttcat
    21181 ttgtaagatt gtttcttcat ctttgatgac tttttctgcg ccattaacta atgtctcgta
    21241 aatcccttcg tagacacggc tgtaatcacc aacctcggaa attacttttt cttcatgata
    21301 actcccttgt tcatctatat aggtcaatac accataatgt tcgggcaaat cgataccaaa
    21361 gtcttcatga tcaggcatgt aaaacatttt cagatgttct tcttgtcgat cttttgtttg
    21421 tttcacgaac atcccttttt ttccgtaaac gacgaaactt ggacgttctt tgatgcggaa
    21481 ataactggat tttatcgaaa ttttcatatt tccatagaaa aaatccagat cgaagtaatc
    21541 attcattcgt cctttcccta aaagttgacg tacatcatag ctgatatcat ctggttcgcc
    21601 gaaataagaa agaacttgat ctactgtatg acaagcatgt ccatataaat agctgttcgc
    21661 agtagaaaat tcatgcgtat tttctggtac ttccggtctg aaataatcat aatgcatttc
    21721 tacttctaaa atatccccta attttccgct ttcgattact ttttggactg tcaagaagtc
    21781 agaatcgaac cgtctgtttt ggtaacattg gataaataga cctttttctt ttgctaatgc
    21841 aaataactct tctgcttcct ttgacgtttc tgcaaaaggt ttctctacta gtacattctt
    21901 accatgttcg agtgccattt ttccatattc ataatgaaat tgactaggtg tagtgatgac
    21961 aactaattgg atctcaggat catcccaaag atcagacagc tgatcagtat attcgatgtc
    22021 ttcgaatgtt ttccacactg gttcagatgg ggaaaaaatt tttttgatat tgattttgtt
    22081 cgtaagtttt cttgaaaaag gcaaatgata gcgatttgta ctctttccat tgccaagata
    22141 agcgattgta agcatgagtg gtgcctcctt ttattgatgt tttcagtata tcctagaaaa
    22201 gcgcagaaag tatttataat agctattctg gtaaaaaagt tcagaaaaac acttgttatc
    22261 attcggtttg gtgaaattaa tgttcaaagt aaggataaaa atcaaaaaga tagtagcgga
    22321 accaagtgtt tatcagtaag atagaatttg gaggtcagat catgcttatt gaagaaaaac
    22381 taacaaaaca agagttgttt accacgacag agaaacggat tgtcgattac atccgtagga
    22441 atatcgaagc tgcagtctac atgacgattg aagaattagc aaaagctacg tatacttccc
    22501 attcagctat cattcgttta tgcaaaaaaa tgggattcag cggatttaaa gaattccgtt
    22561 tcgagctggg gcgggaagtt catcaattga ttgctcagtt caatcaaacg attgatccta
    22621 attttccttt ttcaagtgaa gatagcactc agcaaattgc aaagaaaatg gcggatttat
    22681 cgatccagtc aataaaaaaa gcacaattgc agattgaaca tgaagattta gatcaaatcg
    22741 gtaaagtatt gaacaaggca cagcgaatct ttctttttgc aaaaggtgat tctcaaatca
    22801 cggcaaggaa attccagaat aaaatggtca agttgaataa gtttttgatc atggctgaag
    22861 aatacagtga ttcttcttgg aatgcagcca atttgactag cgaggattgc gccattttta
    22921 ttagctacag cggtcgcatc catcattatg aacgaataat gacctatttg aagcatgtag
    22981 aggcccctac cttgctgatt accggaaatc agcattcaga aatggccaaa caggctgata
    23041 tgtgtcttgt tatatcgcag gaggaatatg attttgctaa agtagcgact ttctcctctc
    23101 aaattgcctt cgattacgta ttaaatacgt tattctcagt gatatattct caaaattttg
    23161 aagagaatat ccgcaacttg aaaaacaaac aagaattgat gcaaaaaggc cttcttaaag
    23221 aatatcggaa aacttaagaa aagatcctca tttagtaata agaaagatag tgtttgatag
    23281 gctctctttg atatcatcat aaaaacaggc tgaagttttc tttttcaaac ctcagcctgt
    23341 ttgtttacgg aacgattcga atagatatat tatagaaatg aggaatagct aacttctgtt
    23401 tcttattttg aacgattgac gaaaaagata tataagaagg aggaaaatca gtatacatgc
    23461 agctaacaat ccatacagga aaaatcgttt gttctcggat actttctgta cttctttttg
    23521 aacttctttt gcatgataag gaacccggtg ccctctgaca agtaagcgat gactgttgac
    23581 catataaggt gtacaagtta ggagtgtcac gaaatcctgc cctttttcga ttctcagatc
    23641 ttttgtatca gtcggttcta ccgtcttgat ctgatcgact tgataagcga gtgtccgtcc
    23701 attgatttcg atatagaaag aatcacctaa agtcagtttc ggtaagtctg tgaacaactt
    23761 tgcttgaggc agtcctcgat ggctggaaat aacggcatgt gtattttctc cccccgtagg
    23821 ataagacgtt ccttccaata atgaggcacc tttttctagc agcacagtat tcgtttcatc
    23881 aaaaattggc aaatggacat tgatctcagg gatcgtcaaa ataccgatcg tatgtttctc
    23941 aaaataggtt ctatcctttt tcgcaggttc ttttttctta gtaaagggat ctgcacctgg
    24001 atttccacct tcttttgcta attgctggtt tttctttgtc atgttttctt ggattttagc
    24061 catgacttct tcattttctt tgaccgcttg ttgctgataa tgagagatga tctgctgatc
    24121 cagataatta ttcagcgcgt cgctaacaaa tggataagct agtatcccta ttcctgagag
    24181 aagcaagaaa agcatcagca gatctagaag tttccttttc tttggtttca tttgtgttct
    24241 ccttgtgatg actgtttgat aataaaagaa aaaggagcat ttgcgtaatc cgctgctcct
    24301 ttttccaatt cttgcaaaaa gactaaatct gactgtgctt gcgtctagca aagtacagtc
    24361 cagcaatcag tagaaggact actcctgcac cgatatacac atagattccc ttaccgcctg
    24421 ttgaaggaag tgtacctttg tgtttatttg gtattttttc aggagaaatc aactgtcctg
    24481 ctgttacata agattgttca tcgatagtaa atgctacacg gtttgttaat ggaacatatt
    24541 tttctggcgc tttcgtttct tccagatagt acgtgccata ttttagacct gtcacatcga
    24601 ttaaaccgtt acttgtggtt gtaaaaaccg ttgctgattc tttcgccgat acccagctga
    24661 cggcttttgt ggatgggtcg atcgataaat atttcgctgt gtcactatct tgatcacgaa
    24721 cgacgaattc tgctccagca agtgtttggt ctgatgtaac gtcaccatct actttaacaa
    24781 atcgtttgcc cccagtaacg acatcgactg acggtggtgt ttgatcattt gtatgcccgt
    24841 tatcgacatt tgcttggtta gaaaatcctt tggttggatc tgctttttcg ttcaaatgca
    24901 tatagtaaac gaatttcaat gtaccgccag gagttaatga aggaatataa ttcggatcaa
    24961 ctgaaaccgt gaatccgtct gtttgctcag tgacagaata atttactggg tcgatttctt
    25021 tatttccatc atataaggca taagcatacg ttcctgaaga atcattatca aatgttaaag
    25081 cagcgtcatg agtatcgata agtttgaatg ttgtgtactt gttttgcgtt ccttctttat
    25141 cagcgatacc taatgggata ttgacagaaa tctcatattg aattttttca ccgattgcga
    25201 catcttttcc attcaattga ggtgttgttt tatcaacttt agacgtatca tttttgatgg
    25261 tcttttctac tggtgtttgg ctatttgcca ggatctcaaa aggcgtgatt gtttgctttt
    25321 caatcatttc cgcattatca ggagctttta cttcttctaa attatatttt ccaacttcta
    25381 aatgattaac gatcaactgg cctttttcaa tagatgcctc ggcaaagtca ttgttgccga
    25441 tgtcataaga atgaccagta atgaaatgtt tggctttggt ttgatcagtt gtccaagtgt
    25501 acaatccatc tgtgacactt tggatgtatt ttttgacgct tggtgttcct tcttctttag
    25561 aaataataaa ttctgctcca tttagtgctt cgttttcggc agtaccgatt tttgtaactt
    25621 tcaacgttcc atcattaccg actgtatttt tagggtagag atggatagta tctagttctt
    25681 ctgtcccgta tttataagag ccatctgctt gtttgatcat ctcatataca gggaaagcta
    25741 aaaccatgtt tgcggcagct gacactccgt cttttggttc ttctttgatc gtgtagactg
    25801 catctttccc attttgtttt ttaggtaaag ataaagtgac atttccatca gcatctgtcg
    25861 ttcctgaagc aactggtgta ccaggagtca aagactggac tgcttgtttt gctgcatcca
    25921 cggacgctcc tttatctcgt tgcgcataaa attcttgagt gacgttataa actgaaaatg
    25981 aaatatcggc taatccttgg tattgatcga attcgctcat ttctttcccg ctgttttgga
    26041 ttaaaggatc gggtaaatca gtcattttct ttttatgaag gatgacttga gctgctgtct
    26101 cctctgcaaa cacttttttt gcgccgaagc tgtttgtgag taatggtaaa ataaggaaaa
    26161 ggactcctaa cataacgttt atttttttat gatttttcat tttttcactc ctgttctttt
    26221 tttgataaac aaccagattg caaagcttgc gaataccata ccaacaacgc ctaaaagtgt
    26281 atttttcgtt tcgtttgttt ttggaagaaa tggacggcta ttttcttttt tattgactac
    26341 tttcaatata ctgtccgcct ctttatcctc agaaagaaaa gttgaaatgg taaacggaat
    26401 ttctgtttca cttaaaatat aaccagaagg tgcttcgatc tcttttaatc gataacttcc
    26461 tgttttcaac ccggaaattg aaaaagcgcc atttttatcg gaaatcaggt gaagcgcatc
    26521 tgattcgttc tttgtccact tgtatccgtt ggctgtttgc tggaggtaat ttcctgcttt
    26581 gtttttaata aggaatactg cgccagctaa tcgtttatct ggtttatttg tctcgatttt
    26641 gacaaattgc ttactccctg tatatacaga gactttcttt gtactgacac caaaaccaaa
    26701 atccagtgtt ccgttgttgt tgatttcctt gttcgcctgt gctgtactgc ttagctgcat
    26761 ctgataagaa atggtcagtt tttttccagc atatttttcc aacgaaggga tcgaaaataa
    26821 aacatcaaat ccgtgaggtt tcttgtgtat gacaaagcct tctgtcagct tttctccatt
    26881 cgatgaaatc gttaaactat taggtaaaaa cgttaatgct tcatccgcac tatctgacaa
    26941 ttcgaacttt tgatagtcaa ggatatttac aggaattgtc gtatgcaagg aataagtgat
    27001 tttttcacca atcgtaaaat catttctagg ctcttctaat accttctcaa aaggtggatc
    27061 agggtagtcg ttttcttcat ttttaggata caaatggata gttgaaagtt tttgattgtt
    27121 ttgtccatgt acaggaagaa caactaccat attctctgcc ttttctttga cgacttctgg
    27181 tactttggat tcaatgaaaa gatacgcttt atctcttttt tcttgatctt tagcggccag
    27241 agaaaaagaa gcgataccat cttcgttgtt aagagttgta gttgttgctt ctgcggtaaa
    27301 cataccggaa gacgcaccgt tttttgcgat ctctgcttgt gcttcttcta ccgttttgcc
    27361 cttttcccgt agatggtaaa aagaatctgt gacatcataa acttggaatg tgacaccatt
    27421 taatcctcga tacgtttgta ataaagcttt ttcttgtccg tcatttggat gatttttcgg
    27481 cagttgcccg tttgggaaaa gcaatttgtg taaagtaatc tgaaccattt ctgtttctgt
    27541 ttctgttgct acctgagtaa aagctggttt aaatagtaac aagaagagac acatactaag
    27601 ccaaccaagt tttttcatat ttagctgccc cctctttctt ttttcaaaaa aagataccaa
    27661 atagaaaacg cacaaccaat catccctact agatagattc ctaaacggcc aattcctccc
    27721 gtttcaggta atggtacttt tgcctgattc gtgatgtcta aagaaatctg gttgtttttg
    27781 gctcctggtg acagaacaga ttcatgatct tgtccatcca cttgaattga cccatcttcg
    27841 tgtataacta tagtaactgg ctcttttaga ccttggtatc cttctggtgt aaaagtttcg
    27901 gtcagcgtat aagttccagg ttttaaattt tcgaatagaa aggtatctgt ttcttgtcca
    27961 tcttttggcg attctaagtc catttctggt ccctgcagtc tgaattttgc tccttttaat
    28021 gtctgtccgt tgtcttcttt tttgtgtacc gttagatcaa acgcttttag tcgattttga
    28081 tgagtgagcg tccatacatt atttgtcatc tcggtatttt ttcccgtgta aatgattttt
    28141 ccatatttat cgatttggaa ttcataattt cccggcgaag tatcatatcc ggcaggacca
    28201 gctgtttcaa cgactcgata tttaccaggt gtgagtgaat caaaactggc aagacctttt
    28261 tcattcgttg tttggctgtc aattggctga taagtaccat ttgcttcttt tttctgtagg
    28321 gaaaatgctg ctcctttaag atttatctct ttgtccgtcc cttgcatcgc atacttacgt
    28381 actgctacag gaacttcaac aaaaggattt tcaatagtca actggatcgt atcatcggaa
    28441 gtagtgactg tttttccatc aatggttacc gtaccatcag aagcgatctt gatttcccaa
    28501 gtagtctttt tgcttaatcc atgcccttct ggagcttttg tttctgtcag cgtataggtc
    28561 atttcttttt gcaatttgac attttctgga agagaatagg ttccgtcgcc atgatctgtt
    28621 agtaaagtat caatagaatc tcctgttaat ttgaaaacag cgccaataag atccttttca
    28681 cctgtagagg aatttttcgt tattttcaag tttaacggtg tgaattgttt agtatttttc
    28741 catgtcattg catctatttg ttgagaatcg tatcctggta caggtaattc tttgattgtt
    28801 tggtaactga atgcttgacc ttgattattg tattgaggta gtgataatat ctcttgataa
    28861 ctttctccgc tatttgcaga taatttgtca atgtctgcac gttcccacgt atttgttgta
    28921 tctttagctg gtttaatgat tcgaatatat ccgtttttcc aagctgcagc atttgtcgta
    28981 tgttcccgtt gaatctcaaa agtaacttga tctggacgat cagctagatt gttgtcaaat
    29041 tcttcccata actttttgat gtgaagactg actccgggag ctttagcaga tggtataccg
    29101 aattcagcta attcattggt atcaatgttt ggctggaaag ttgtcctgcc gttcatttga
    29161 taccagaaat ttggatggaa gtcctcattt tctgtttgga ttctcacttg gtaatggatt
    29221 tggatttctt ggtcttttcc taaatagatc tgattgctct tgatggtatt tccttctatg
    29281 gaaatagatg gagtgaccgt tgtaggactt gtccccacac tcttgactga aagtgtacca
    29341 ggctgataaa tgaatggttc tgcgattgga tcatttattt gtccattgct tacagttgct
    29401 gagatctgta cagctttttt ggctaggtat tcagagatat ctgttgcatg atcagctgat
    29461 tcatagtaaa gatcgccttt ttcatctgat gaaaccattt gtcgcatacg agactctact
    29521 tctgcttttg agagaccagc tgccggatcg ctttgaagtt ggatgccaag accatgtatt
    29581 tcgattcctc gttctttgag tgccatcgct tctccgatgg ttgcgataaa cgtactgtcg
    29641 attcttctgg atagattgtt ttggtcaggt gcatcataga ttcttgagat tagagaagta
    29701 tttcccggcc gatcttgcgt attagaaaac tgagttccgt aataattgct gcttgattgt
    29761 gcgtgtaccc gctgtacttt ataggaaaat gttggtacac catccgtcag caaaacgatc
    29821 acttttttat gtccatttgg aacggatagc atgcttcctg catctcttag tgctttttgt
    29881 gtaaaagtac caccatttgt ccgtgaaggt gtaatggatt ttacttgatt tttcactgaa
    29941 tcaaatgaac ccatctgtac tgcaccgtta ctgtagctat atccttcgct tgagtagccg
    30001 acatatccca tattgatttt gtctgtgata ccgctatctg ctaaagtatc gacaaaacga
    30061 tcgacaccaa tctttacttc accgatccga ttattgtcgt tcatacttcc tgaccagtct
    30121 acgaccaata cgagatcaag aggcgtgata tctttttgta catttccacg agcattcaaa
    30181 taaacatcaa acaatccggg tgtacttgtt tcctttgcat actttcgtat cgcatagtct
    30241 gcttgattac cggttcctcc atattcgata tacgaatggg tccgatcatt aggatctcca
    30301 ttccaagaat ttataccatc ccattgattt gttccgtttt tattgccttg atgatcaaga
    30361 acatttgtat ttcctgtagg ttgccaactg gctttaggat aagtgccctg atcatctgtt
    30421 gtatattctg taggaatcgt ttgattagat ggcacactca cagctggcaa ggataatgta
    30481 ggcaatgtaa atgggctgac agaattttcg ttttcttttg atgattcttc ttctatttcg
    30541 tctggctttg gtactgacac atctgtatct ttcttatttt ctgtctcatc aataatgtca
    30601 tattctttat tatcgacgat cagcttgttt gcctggctgt ggtaaagtgc tggatcgatt
    30661 ggaaaactca gatcgatagt ttgtttagtg tttgatgaga tttccaacgt aattttcccg
    30721 ctatttacct gataagcata gccatctcct tcttttcttt ccacatcact taagtaattt
    30781 tcgactagac caatttcttt ttctatcttt tcagcactgg tgttattcgc atttagcttg
    30841 atgttcagtg ctttttttcc agactgatcc gaaatgactt cagctgaaat tgtttctaaa
    30901 actgttggat catctgtagt gtctgctatt gcagataatg gcacaaagtt tgataaactc
    30961 aatattatca gcatgaaaat aactttcagt ttcttccctg tggtcgtcat ttttcccctc
    31021 ctttttcttt ttttctgttt tgacgtgaat agtgaaaatt attaaaataa acaggcgtta
    31081 cataatttta tttattttaa tatgataatt attatacgaa gggcgctaaa gggaatcaca
    31141 ccaattttca taccaattgg tttcatttat gaagttgatt ccttcgatta atgagcgttt
    31201 cattgaaaga aaagggttga gggagaaaaa catatgatca ggttgttgga aggaaactat
    31261 agaaagatgg aaaaaatcat tcgaattctg tccatttctg acaagtggat gactaaaaaa
    31321 gaacttgcca aacgaattgg tagttcagaa tctacatttg taaggtatat cgaagagatc
    31381 aaaatccgct ggggaacgga tgtaacgatt caatcttcac aaaaattggg gtatcgtctt
    31441 gaacatttga atgtaccagc ttatttaaat gttctgatgg aaatgtcgca aagttctaca
    31501 aacagtcgtt tattaaatga gattattgtc aatccgggta aaacgattga gtattattgt
    31561 gaaaaaattt ccatcagccg gtcttctttt acacgtaagt tgaaacaatg caatcaaatt
    31621 ttagaaagct atttattaaa agtgatcgtt gaccaaggtt ttcagttaac tagccttaaa
    31681 agtgagctcc agttgcgtat ctttgttacc tttttctttt taagttacta cggaagacgc
    31741 acgttgccat ttccaatcga aaaaaagaag atcaaagaaa ttatggtcaa gaataattgt
    31801 cagatgaatt tgtttcagga agaaagccca tatgagcaat ctttctttat tatgtatttt
    31861 gttgtgcatc tcatgagaga atcccaagga actccatttg aatggaaagc tggagactct
    31921 tatgtacagt tgataaatat cgataaaaaa gacttcttaa tcttgaaaaa tctcttagga
    31981 aaattgaagt acagcagcta tataaaagta gtgaactatt ttgtcaatac gattttttca
    32041 gaattttcgg agaaagaaaa aaatgaaatc agtgagcaga tacgaaaaaa tatgtcctac
    32101 tgttcgtttt catccgctga ttgcaaagaa aaaaacgaag aatatgttgt tcaattatta
    32161 acgcaaattt attttttctc ccgtattttc ccatacgata cgactcattt gacgatccgt
    32221 gcaacttact ttgcacagca gatgcaacac cgccagatac gtctatatgc tggatttcgt
    32281 gcagaacttc aattttattc cactctactg aatcagaatc tgatttatta tttatctagc
    32341 tatatctttt ggatgcttat cggtaatcct gaaattcacc attatacttc tgataaaaaa
    32401 atactgatta tcagcgactt aagtcttcga catagtcaat atattgaaga atatatcagt
    32461 gatattcttg ctgtgcataa aattcactct gagacaactg ctattactga agatcagctg
    32521 tcgaaataca atcttctcga gtatgatctg atcgttacga atcaacccat tttgaatgcc
    32581 catgttcctc atatattgat tgatgatagt gtgtcattcg caaatgaaga agaactcatc
    32641 cgcttattcg agttatgaat aatcaaaaaa cagctatgta taaaccaccc tgataaaaat
    32701 ttcaggacaa atacacatag ctgtttcttt ttattgtatt ccttcagcaa tccaacgaga
    32761 tttatagcga aagtctttta acaactcttc ttttgtccaa ttatttgcaa tgagatccgt
    32821 caaagtgttc attacgaggg tcatggaaaa gtataggaaa ctaactgctt cttccgacga
    32881 ttgaatggag aaatgattga ttcttaataa ttccagccat ttttcgttca ttggagaatc
    32941 agccgatagt tctctacgtt tggttgtctg ctgatcattt cgctgggtta acaagtataa
    33001 cctttgccaa tagatacttt cgtgatctaa gtcgcagcac cactctaaat atttttcgat
    33061 ccccaaaaag aaatgttgtt tgttttcgct aatgaatttt aagatatctt gatggactat
    33121 gtgagaactg cttttgatcg tatacaaata agcatcttcc aaatcttgaa aatatttata
    33181 aaaagcaccg cgcgacatcc ccatcttgtc aacgatttct gagactttga cttggctgat
    33241 gtgctgtgag caaaatatct ccagtaatag gtcatcgatt ctctgtttct ttgcgttcga
    33301 taaattgaaa aaagtttctt ttggcatcca catctctcct ttatctctga ttatacgtgg
    33361 atagcccttc attgtcatga aggattcatt ccttcttttt ctcttcttct gtcaagtacc
    33421 catcttccat tcgataaacg cgatcactcc attgaatcat acggacatca tgagtaacca
    33481 taatagtcgc tttttgtttc tcatgagctt ctttaactag taatttgacg acttcatatg
    33541 catgatctgt atccaaacta gctgttggtt cgtctgctaa gatcaagctt ggctcattat
    33601 ataaagcgcg agcaattgcc actctctgtc tttctccacc agaaaggtct ttaggataac
    33661 tatttttaag tgactggata tctaaagatt gcagcaagga atcgattttt gttgaatctt
    33721 ctttttttga agtaacttgg tcaactagtt caaattgttc ctttaccgtt aaaaaaggaa
    33781 tcaaattaga gctttggaga ataaatccta tttctttaaa gcgtaaatct gctcgttttt
    33841 tttctggaag ctcagttagt gagtgaccgt tgatcactat ttctccggaa gtcggtgttt
    33901 gcaatcctcc tgaaatggta aggaaagtac ttttacctga accagaagga ccaatgatgc
    33961 tgacgaattc tccttccttt acataaaaat caattgcttt caaagctgag acctttgtat
    34021 gaccagaacc aaatgttttg gtaacttttt tcatcgttaa tatttttttc atttttatta
    34081 acctcctatt gctgtaattg ggtctacttt ggttatcgta cgtatcgaaa acaatccgcc
    34141 tagcattgcg acaatgatca aaatcccact atatagcaac cattgtgacc aaaaaacagc
    34201 aaaaggcatc gcgcttggta aaatcagact agtcaaataa gcaaacaaaa gagcaagcag
    34261 aacgcctacc accccaacaa taaaggactg tgctactaaa gatttagcga tgaagaaatt
    34321 tttgatccct tgtgctttca ttactccaaa aatagcagtc ttctgaagtg tcatcacgta
    34381 catgaaaata ccgaccacgg cagcaaccac taagaacaaa aagtatatca tcgcatttaa
    34441 ggtcatattt tgggcagaat agccaggaat actttcaatg aatgtcccaa tagttaataa
    34501 ctgtagaccc ttcgatgctt tttctttcga aattttagct gaatccttta ttacgatccc
    34561 attgatcggc tgttcttctt cagaagcaaa cggctgttgt ccatacttca aagcagtcca
    34621 cgtatctagg gacgcataga tgacgggaga aactgtgtaa taagtttcag gaaagatacc
    34681 gacaatcgtc aaaggatcgt cgtaactgcc aattttgatc gtatctccaa ttttatagcc
    34741 tgaatcagcc agattttgag agatgatgat ttcattcttt gttttaaatt catgcccttt
    34801 cgtgagtttt ggtaataaaa atgcttcttt cgttgtgccg aaaactgtaa cattttcttt
    34861 gttttttcct ttgattgctc cgctgtacaa tccgattggt gctttctctc ctccttcaat
    34921 gtatttcaga tctcctcttg ttaattgtga agctgcaaat acttgatttg catcctccga
    34981 caaaacaatc tcttgtgcgt cccaatcatc gattgctttt ttaaactctt ctgaaagtcc
    35041 attcgctagt cctgacaaca tgaatacgac atacgcaatc aagaacataa taccgacgat
    35101 caagccatat cgtaatttag aatagcgaat ttctttgata gctagaaaca tttgtttttc
    35161 ctccctatga tagattgata gtgacaaaat gtcacttgtt gaatagtgta actctaaagt
    35221 gacaatttgt cacttgtgtt gtttttttct tatcaataaa ttgtctaaga catctttcga
    35281 taaaacctta acatgagtca taaaaaattt atttaagcct tatttatgct ttccatgata
    35341 aactaaagtg accaaatcaa tgaggtggga aaatgattaa actattagta gtagaagacg
    35401 ataaagtatt atccaataat ataaaagaaa tactcgctgc aatcggcgag ataacacaag
    35461 tttatgatgg tagtgaagga ctgttcgagt tggaaagtgg agtttatgat cttgcaatct
    35521 tggatttgat gatgccggaa atgaatggat atgaagtgtt agcaaaaata cggaaagaaa
    35581 aaatacagac tccagtattg attttgactg caaaagatag tttggaagat aagattgaag
    35641 gatttgaacg aggtgcagat gattatctaa caaaaccttt ctatcgagaa gaactgatca
    35701 tgcgcgtgaa agcattattg aaacgctcgc taggtctctt tgatgaaaat gttctgaagt
    35761 ataaggatat cagttgtgat ttaaaaagca atgaagtctt gtataaagaa caagttttgc
    35821 ccatacaagg aaaagagttt gaattgctcg tttatttttt acaaaataag ggaatcattt
    35881 tgacaaaaga gcagatattt gaccggatct ggggatttga ttctgatacg acgattacag
    35941 ttattgaagt ttatatgagc aacttacgaa aacatctgcg aagtactgga ttggataaag
    36001 aaatcaaaac cttacgtaat gtcggttata tactgcaaag ggtgtgatgc aatgaatgga
    36061 gatattacac gaaagcaacg tagtcgcttt tttgcgacaa atgtagcagc atttgctttt
    36121 atcttttttc ttttgggatt cattacttta cagatattaa accaatctgc atacagagaa
    36181 acagatctgt cattagagaa aataaatttt gattctcgaa cagtacaatt agagatacaa
    36241 agatatcagc aaggcgatcc ctttttaaac ccgactcaaa cggaacaaaa cagaacaaac
    36301 atggaacagc cattaatgga accatcaaat gaccgtttta acactcaagt gatcttgtgg
    36361 tctaaaaatg gtgagatatt gaataaagca gctattggtg ggcgtttcaa ccaaatcaaa
    36421 gaattaaaaa tggatacgaa aaatctgaat accatccaaa atattgaatt aaatgaagac
    36481 agtggctcat ctgccctctc tttccgttcg attacgaaaa aagcggagac gaatcaagat
    36541 ggcgttgcct acatccaaat tttagaaaat acaaatcaaa tcaagaattc attagcgact
    36601 tttcgcacga ttatgatttt gtgtatgatt gttttttgga ttctctctat tggtatcagt
    36661 tattacttgt ctagtctaag catgcgccct attattcttt cttggagaag acaaaaagaa
    36721 tttgttgaga atgcttctca tgaattgcgc acaccgttaa cgattattca aaatagtttg
    36781 gaacatcttt ttaccaagcc taatcatact attattgaag aatccgaaag catcgctcaa
    36841 gcattgtcag agacgaggcg cctcactgga ttgacttcgg atttgctgac aatcgctcgg
    36901 aatgattctg atcagcagct gctttcaaag caaatgatca atacacaaga atatatcgaa
    36961 caacttgtta aaccatttca agagatggcc atcatagatg gaaaagagtt tattctccat
    37021 aacgatgcaa cattagaagt accaattgat gaaaaaaaaa tccatcaagt tttggttatt
    37081 ttgctggata atgcattaaa atatacgaat ccagaagata agatcatttt ggattcatct
    37141 gttaaccata aagaatggct gattcacgta aagaatactg gcccttctat cagtgaagaa
    37201 gatcaagcaa ggatttttga tcgattttat cgggaagatc aatctcgggc gaaagagact
    37261 ggcggatacg ggcttggctt ggcaattgca aaacaggtaa tcgaacagca tcatggacag
    37321 atttctgtat ccaattatct tccacaagga gtcgatttta aagtccaatt gccgttgaag
    37381 cctaaaaaat aatgcttatc cctaaaaaag ggctgtgaca aaaatcttgt cacagccctt
    37441 ttttgttgcg aataaacggt gttccagaag tagcttcttc ggaaataagc cgaaatttct
    37501 ttaagctgac cacttctgtc acaacctctt tagcttacgc ttacgatttt ttattgcggt
    37561 cttcaatgtg tacatcaccg cttgtgtcaa tatccaattc ttctcggcta acatcttctg
    37621 tcactttttc aacatcttca tgttcttctt tatgaatccc aacttcttca cgaataactg
    37681 tatgtttgct tacatctact tgctcttctt tgattgggat agtaatcgtg tcatcttcca
    37741 atttttcgtc tgttccctgt gaagattgat ctgttactgg tttgcgttcg atgacgattt
    37801 cttcacgttt gaccggtact tctaccgttt cagtatcgtt gaccacatgt ttgtggatgt
    37861 cgacttctcc agttgtcaca tcatgtgtat ttacatctaa tttttcttct tttagttgaa
    37921 ttgtatcatc atctagattt ggtttgcgat cgatgtcttc agtcttgctt gtacccattg
    37981 tatcagtctc gccaggtacg tccatcggat cagttcctgg tgtcatattc aatggatccg
    38041 ttgtattttg aaagcctgtc gtgccaccta cgtccattgg atctgttcca gtgccagttt
    38101 ctaatccagt ccccattgtg cttgctgggc taacagtatc ttgtgtatcc attgtatctt
    38161 ctgttgtttc tggctgacga tagcctttta ctgtgatgac aaattttcca tcagaaatat
    38221 ctcctctata ttgatataaa ggatcatttt ctggtgtagc cgtctcagaa tcataggtat
    38281 caaaagaaaa agtatctttt attttttccc ataaagaacg gtcttcttca ccatccatcc
    38341 gattttcatc tgtttcaaca tctataccag acaatccgtc aaatcctagt ctagatgctg
    38401 cttcatcagt agtataaata acgatatcat ctcgttcata cccttcgtta cgcaaccgtt
    38461 caattactga taatgcttct tccctcgtac tatagcttcc gatgaccgtt aaatttttct
    38521 cttccatgta aattcctcct agcaaataga tttatgattg gtatattgtt acttataaat
    38581 ttattctata ggagacgatt gttttctaca aatatttaga ttggaatttg attaaaacaa
    38641 tttaattgaa atttgaaaac atttatgtta atgaaagtca tattctaata aaatgtcatt
    38701 tgtttctata tgaattttta tgaaaaattt catataattt ccactcgttg tgatggttta
    38761 atttatccaa tccatcaata agcaaacaac ttatataaaa aaggattaat tctcaatata
    38821 attgctccta ccatagaata aatcttcatg taaagtaatg aagtaccaac tatttctaat
    38881 acttctttaa gaattatccc aacaatatcg ttagacaaac cattattgaa taaatggaga
    38941 gccaatcgat tctgaaaaaa tgttttctaa tttttcggaa ataaaagaaa gagcctcctg
    39001 ccattagaac gaaccaaatc catttcattg ctcatgaagt aaccacatag ctaaaaagag
    39061 gataaatcaa ttaaaagatc aaaagaatga ctgcactaat aaaataacta aaaatgtatc
    39121 ttcttacttc atatctttta gctagtactt agttaaaaat ttgcttccta agatcaagac
    39181 aaactgtcta tcttcatttg atgaaatgcg gtaaaatata gaaaatgaac actattggag
    39241 ggaatttgta atggacagca aaactagaaa acaactgcaa gaaaagttaa agcaacgtac
    39301 acacttgctc ccttccgtct ttcaacagtt tttagaagaa aatcgttctt ccctttcatt
    39361 aggcagtcgc tatgaatacg caaaagattt tcatctgttt actgattatt tgagaaaagt
    39421 attttctgaa gaagtggatg atcagctgat tatccattta gaaaaacaag attatcagaa
    39481 tttcttagct aaaatctatc gacatactcg ttcgttccaa actacagcga accaagaaac
    39541 ggagcaattg tttgaaaata gctattacgg gatttcaagg aagcaatatt cattaaatca
    39601 ttttttacga tttttgtatc aaaaaggact gattgaagaa gagatttctc ttttgggtac
    39661 aagtttaccg gaaacaacca aagcagctcc gcgatatatc gatgcagaac ttttcaaaga
    39721 ttttgagcat ttgtttatcc cgattgaagg ctttcaaaca agcagagaag ctagctttga
    39781 agaaaagaat agaccgagga accttgcaat tactgccatt cttctgtatt gcggattaaa
    39841 gatccatgaa gtagtagaac ttaaacgtaa agatgtatct ttgaccaagc agattcttaa
    39901 tctgaataga aaagaaaaca gactgaataa agtaccaata ccaaaagaag cgatgccatt
    39961 tctggagaac tattttaagt taacagcaca aaatacggac gaaaattctt ttgtttttcg
    40021 ttcctcacat gatcagcaaa tttctcctcg gactattcgc caaattttga gcaaaattac
    40081 agtatataaa aacgtatcgc cagaactatg cagaaaaaca tatcgttatt atgcagaact
    40141 atatttagat gatgctgacg cggtaaatta tttatgtggt aatcgatacc tctcatccca
    40201 ttcttttcaa gatatctggg aaaagatggt tgatttttct tatcacgaat tagcggcatt
    40261 cgttcgggtt tcttccatat aaaacaaatg tatgatcttt ttttcacaat aaactatgtt
    40321 attatataaa ggattatgtt acgattatct tgaatttgta tattacttaa caaaagataa
    40381 tggagtgtga atgatggaga ccataacgta ttcccctttg aacttattta cgaattttca
    40441 agaagctgca gctaactttt ccgagacccc gattatcttt gatgagccac tttcagcttt
    40501 cccagaatta ggacttgaaa caacttacaa aaaatgctgc gaaagtgtgt tgaaacgggc
    40561 ttatcaattg gctcatcttg gagtgaaatc cggagacaaa gttattattt ataagagttc
    40621 cgcttttgac acataccttc tagctgtttc tgtgtcttac cttggtgcag tgcctgtcat
    40681 gacttcttat catctaccaa caacaacaat ggaagtattt atcgatcgtt tagaagaccc
    40741 tttcatttta tttgatgatg agacaaaaaa gcgagtgaga gaaatttcaa atggaacaaa
    40801 atcaaaacag attcctattc tttatttaat ggaacaacct gctttgtctg tttctcaatc
    40861 atttttagac aaaaatgaaa tttcttatat gacccatact tctggtacaa ctggtatccc
    40921 taaacttatt tgtcattctg ctcattctat gggatggcgg acgaaatggc aaaaaacagt
    40981 tttcacaaaa atttcggaaa agaaattaat cgcttttcac atctctccag tgcattccag
    41041 attcaatatt ggtatctctt ctttgatgtc catgggattt cctatgatgc ctttagcaaa
    41101 cgcacaaagt tcaaaagttg ttcatatgtt ggaagctcac cggccaattg cactagagac
    41161 ccatccgaat aactttgttc agtggcgttt tactgctaag gagcatccag aagctttttc
    41221 gggtatccga tactatcact ccacttttga tgctataaac aaccagacaa tgaagacatt
    41281 tttgcgaacg tcgatggcac aggatgcggt atttcttcaa gtatatggcc aaagtgaatg
    41341 cggaccaatg attcttaagg ctcatacact agagagtttg aaaacaagtg atgcaagaga
    41401 tatgggcgtt ggactggaag acatgacaag cgctcggatt acagattcag ttgggaatgt
    41461 cctacctgaa aacacagatg gtcatataca attactgtct aaaggacggg ctttgactta
    41521 ttacaaggaa gatgcacggt tccaggaaaa tgtttatggc gattggtggg atagcggaga
    41581 ttacggtttt atggataagc atggccacct atttttgaaa gatcggcaag tggatttgat
    41641 cgaaacgatc aatagtacat tagctattga agacttttta ttagattcgt tagatttttt
    41701 ggcagaagta gtgattgtaa gggataaaaa tcattctcca caaccgatta ttgctttagc
    41761 tcctgataaa gaaatggatt ggaatcgatg gtgggagcaa gtccatgaac ttcctcgctt
    41821 gaacgtccct atcatccgtg attttgatgc aatcccgcga acagctacaa tgaaagtgca
    41881 acggcttcaa atcgagaaag aattaaaatc acaaaagtcc taactattta aggaaagaaa
    41941 gatccatttt ggtctttctt ttctttttgt ttttttaaat tggatgagat aaaaatatgc
    42001 caattggctg tttttctttt ttgaaaattt cgttatactc ttattcatgg aggaaaatgt
    42061 tatgaaaaga atcttgacaa ttggcggttc tgatccattt gctggtggag gaatccaaac
    42121 agatttaaaa acttttgaga attttggtct attcggacta agcgccttga cttctatagg
    42181 ggcactagat attaatgagc gatttatgct ggaatctata tctttggaat tgcttaaacg
    42241 acagttagtt tcgattgata aaatgacttc attagatggt atcaaaattg ggttattgaa
    42301 ttcaagtgaa gcaatcatgg ttgttcgaga cttcataaaa acaaaacaaa atatcccaat
    42361 cattttagac cctgttttgg cattcaaaga aactgaagct gtgatcaatc aattgtatat
    42421 ttcgcagctc atcgaagaac tttttccact tgttacgatg attacgccta atttggcaga
    42481 agctgcttta ctttctggtc aagctcttcc ctcttcgtta acagaaatga tccatctcgc
    42541 taaagaactc actgcttcag gaacaaaaaa cattgtgatc aaaggcggca caaggatggc
    42601 tggagatgaa gctgtggatc ttctctattc aattgatcat tatgaggtat tttctcataa
    42661 aaaaatcgac actacaacag taaacggggc cggttgtgcc ttttcttctg caataacagc
    42721 gaatcttgtg ttaggggcag attttaccaa agcagtagca gaaagtaaaa aatttgttgc
    42781 ccattgtatt tctaatggcg tgctgatgaa cgatcatacc ggaagtgtct ggtctggcgg
    42841 tattcaaaaa ggaggaatga gaaatgaaaa ctaagcaatt aacgatctat gctatgctga
    42901 ttgcgttgac agttgccctt tcattaacaa ttttgatccc tgtaccggct acgaatggat
    42961 ttgtgacatt atgcgaggcc gggatctata ccgctgcttt tctttatggt ccaacgggtg
    43021 gattagttgt tggtgcttcc agtggtctat tgatcgattt gatttcagga tatccgcagt
    43081 gggcagtttt ttcattagtg atccacgggt tacaaggata tgttgctggt tctttcagca
    43141 aatcttctac tcgtatgtgg ggattagggc ttttattagg aagcttagtt atgatcatcg
    43201 gctatggtat cgccggttgg attttatatg attggccggc tggagtggct tctataccag
    43261 gaaacatctt ccaaaatctg attggaatgg gacttgcctt tcctataact gcaagtcttc
    43321 aacgtattca agcacggaag caatattaat tgaaaaagaa ggttttagaa aatgaagttg
    43381 tcagaaaaag aattaaaaga ccaattgact gaaatagtta atgacatatt ggctgaggca
    43441 cacttgaaaa aaggtgatct ttttgtttta ggatgcagca caagcgaagt cgttggagga
    43501 catattggaa aaaattccag tgcggaagtt ggtcaatgga tcatccgtac gttaaaagag
    43561 ttgttggatc ctaaagaaat tgcgctagct gtccaaggtt gtgaacattt gaaccgagca
    43621 ttagtagtag aacgaacagt ggcagaagca aaaaatttcg aaattgtctc tgttgttcct
    43681 gcactacatg ctggaggtgc ttgttctgtt gctgcttttg accagttcaa tgatcctgtc
    43741 gaagtggaac acgtagttgg acaagccggt atcgatatcg gtgacacatc aatcggtatg
    43801 catatcaagc atgtccaagt acctgtcaga cctcgtttga aaacccttgg acaagctcac
    43861 gtgacagccc ttcgctctcg tcctaaatat atcggtggtc ctagagcgaa ttattgaaat
    43921 gagtaattga cagagcaatc tgaaaaactt cctcgcaaag tccaactaat gaactttaac
    43981 gaggaagttt tacttgtttg ttttatataa ccgagcttca taaggtgcta gtatgccttt
    44041 agcttcaaat tctccgctag aggaatttga caagagtaat gtccaggact gatctgcttc
    44101 ttttggaagt tcatagtcag caagtgattc tgttaagttt accataataa gatattgttc
    44161 atcttctaaa tatctttcat agacataaag ttgtggatgt tcatgaagat attctttata
    44221 agaaccgtat atcagacctt tattggtttg tctaatgcgg atcatgttct tatagaattg
    44281 caggaccgac tctggttcat attcttgttg ttctacattg atcgttttgg tatttggatt
    44341 agtgatcagc caaggttcat gtgtagaaaa accagcatat tgttccgaag tccattgcat
    44401 gggtgtcctg ctgttgtctc tcgttgtttc acgaatgata tccaatgctt cttctcttgt
    44461 cttaccttcc gcaagcagcc ttttataaag tcttttcgaa tctactgcat caacttgatc
    44521 gatcgattca aagggcatat tcgtcattcc gatttcctgt ccttgataaa taaatggcgt
    44581 accttgcaaa aagaaataca tcatagcgat tgcttttgca gacatagccc aaaaatcggg
    44641 acgagtatct ccaaacactg aaacggcacg aggaaca
//
LOCUS       DACDXO010000018        41244 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E23605_2017
            SAMN12639989-rid9881463.denovo.018, whole genome shotgun sequence.
ACCESSION   DACDXO010000018 DACDXO010000000
VERSION     DACDXO010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639989
            Sequence Read Archive: SRR10023389
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 41244)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41244)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741914.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:42:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,972
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 33 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 17 of 73
            Pseudo Genes (multiple problems)  :: 10 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41244
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639989-rid9881463.denovo.018"
                     /isolate="E23605_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(530..1312)
                     /locus_tag="IWM35_001137"
     CDS             complement(530..1312)
                     /locus_tag="IWM35_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9919003.1"
                     /translation="MENSSNSAEVNVSTSTSEVVKCSQETTSLPEELLGDWQINNKDA
                     GIEISDSKAVFRIGRSQVESKDYYVDVNNKMLLHVYFSFEYDSVKYIEVSIERTGYSE
                     FISKSVGILTMHTKDGQYISLGVKRVSGENNETEAVTEQTMDERIDSSKSNETEVDTG
                     KTIDERIDSFIETYTAIPPTWKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNA
                     VTEQVGKDIPLRLLENGKKDYSVVFKNGQVTDYNEVFKKVIE"
     gene            complement(1377..2213)
                     /locus_tag="IWM35_001138"
     CDS             complement(1377..2213)
                     /locus_tag="IWM35_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP9919004.1"
                     /translation="MRVIKTLNNSLILCLDKQGKEVILMGKGIGFQLKKGDLVPADKI
                     QKVFKLDSLSDALKKDYIKLFEAAPDDLIICVKRIIDYANAKFDFKLNKSLFFTLVDH
                     LKHAIERSQKGIVFQNRLLIEIQKYYPKEFEIGCYAIQLINKKMNVQLPEEEAGNIAF
                     HIVNAQSEQANMEETMLSVRALKDILAMLGYLFPEKKVDKDSMLYLRFVTHLQFFIAR
                     MIKKEELPEKDDFLLASVKQRFPKEYRAALKIKEYVQEEMGASANQDELLYLTLHISR
                     VY"
     gene            complement(2231..3640)
                     /locus_tag="IWM35_001139"
     CDS             complement(2231..3640)
                     /locus_tag="IWM35_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP9919005.1"
                     /translation="MQYKQLSAFPEKFLWGASISAYQAEGAYQEDGKQPSIIDKYQHT
                     EGVANFEVASDFYHHYKEDIQLFKELGLKAFRFSIAWTRIMDIENNKTNQKGISFYHQ
                     VIDECLNQGIEPIVTMYHFDLPYFLEEQGGWLNRATIDYFVDYVQVLLTEYGDKVNYW
                     LTINEQNTMILHPGAIGLPPSGELPSKKELFQINHHVLLAQARVIHLYHQLNLKGKIG
                     PAINLTAMYQATSSPEDAIAAHNWETLRGWSFLDVAVRGKYNYLFENYLNDRGLYPKI
                     EAEDQAILSSGKPDFIAINYYSTATIAASKNDGSDVSARAGDQQIMLGEEGVYRAAEN
                     PYVDKTPYGWVVDPTGLRLTLRKLYDRYDLPILITENGYGAPDIVEEDRRINDQDRID
                     YLAKHILAIQAALTDGVDVFGYLPWSAIDVVSTHQGFNKRYGFIYVDRTDENLKELKR
                     IKKESFFWYQKVIARNGLA"
     gene            complement(3654..5477)
                     /locus_tag="IWM35_001140"
     CDS             complement(3654..5477)
                     /locus_tag="IWM35_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9919006.1"
                     /translation="MDYREVGRNVLQQVGGKENVVSLTHCATRLRFELKDKRKADTKA
                     LEKTLGVISVVDSGGQYQVVIGNEVQTAFKEIQKEVGTSIKKGPSSDNQESIISRLIS
                     VISTTFTPMIPAITGAGMVKAILAVLTLTGVLSDSSQTYIILNTIADAAFYFMPVLLA
                     YGAAIKFDVNPILAITVAGILLHPNISQLFSTGDAVSFLGIPVLAADYAGSVLPIIFT
                     VWIMSYVEPFADRISPSFIKFFTKPMLVFLIMGPLALVVIGPFGTLLNDLIAMGAEWI
                     NGKAAWLIPFLMGGLQPFLVVTGTAWAMTPIATMQLSSTGSEMINGPGMLASNIAQSG
                     ATFAVAAKTKNKELKQLAVSTGITALMGITEPSLYGVTLKLKKPLVASMIGGAIAGLY
                     AGLSGLVRYAFVSPGLAALPAFIGENPMNIVHAIITCVIAFVATFIIAYLIGFDDTSD
                     ESEQTAIAEAVIVENPVKGTVCPISEVNDAVFSKGLLGKGVAIHPEENSIYSPVEGEV
                     ITILESKHAIGLRSKEGIELLIHVGIDTVNLKGEPFEVFVKAGDQVSAGQLLINADFA
                     AIRNAGYDDTVIVVVTNSENYAGISEASGSLKEHEPLMQIS"
     gene            complement(5757..5984)
                     /locus_tag="IWM35_001141"
     CDS             complement(5757..5984)
                     /locus_tag="IWM35_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9919007.1"
                     /translation="MIEIGYCLLIFTKKTSITIVAYIVGMLFIPSFTHQIHQFFPRAE
                     LLKLCDPLYAFLMLSRFWEFPAGYVGMVLLV"
     gene            complement(6320..6646)
                     /locus_tag="IWM35_001142"
     CDS             complement(6320..6646)
                     /locus_tag="IWM35_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9919008.1"
                     /translation="MNKVNMNKKFVIIGIVVLAIIGIIFSKMTGTSGLEKSSKPLVTQ
                     ILQEQYNLNRSCDDVTITHENGDNTYRAKAILDNGSAININIEYYPKKDRIYVEIPYA
                     EVLMLN"
     gene            complement(6692..7054)
                     /locus_tag="IWM35_001143"
     CDS             complement(6692..7054)
                     /locus_tag="IWM35_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010717431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HAP9919009.1"
                     /translation="MYCRKCGKEITDESKYCQFCGALQETDETTGTDTVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVSLAFIPVVFGAVAVIMGYLYREKDEKMGTILM
                     IAGVAGAIFGVLLGMSMY"
     gene            complement(7091..7306)
                     /locus_tag="IWM35_001144"
     CDS             complement(7091..7306)
                     /locus_tag="IWM35_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9919010.1"
                     /translation="MKKVSNLSRYRKEKGLSQTELAKKMNVTQQCVSSWQTGRTIPKP
                     YQMKMLSEILSVPMNELFSDVFNKVNS"
     gene            7433..7768
                     /locus_tag="IWM35_001145"
     CDS             7433..7768
                     /locus_tag="IWM35_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9919011.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAEKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVVVDYEEEFLALYRSLSTEDAVRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            complement(7844..9841)
                     /locus_tag="IWM35_001146"
     CDS             complement(7844..9841)
                     /locus_tag="IWM35_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9919012.1"
                     /translation="METSMINIPIYVDQEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSVEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKESVTCILSGEQLAKKMSSYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLE
                     YLNRLKEEKALGNFNHKRSNVYRLDVFNATSVIQKLNEDILPLLEKIHFTYQYFSYEW
                     VKICFNEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTYLMTEKELINTVC
                     ERLRNVGDNSVKNALIKNRGYFVKQGFTLERASKDIKKLYNVKASSSFLIYLAKGAGI
                     DETIKELILSGAGIVPLYQFTSVTRDDGRRVRKRKDPIVLN"
     gene            complement(9939..10214)
                     /locus_tag="IWM35_001147"
     CDS             complement(9939..10214)
                     /locus_tag="IWM35_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9919013.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMERYKG"
     gene            10446..11180
                     /locus_tag="IWM35_001148"
     CDS             10446..11180
                     /locus_tag="IWM35_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9919014.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIVEEFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDN
                     INKLEQTISSIGKKYTKKKMKGGDTI"
     gene            11219..12361
                     /locus_tag="IWM35_001149"
     CDS             11219..12361
                     /locus_tag="IWM35_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP9919015.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
     gene            complement(12456..13847)
                     /gene="sufB"
                     /locus_tag="IWM35_001150"
     CDS             complement(12456..13847)
                     /gene="sufB"
                     /locus_tag="IWM35_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HAP9919016.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
     gene            complement(13852..14322)
                     /locus_tag="IWM35_001151"
     CDS             complement(13852..14322)
                     /locus_tag="IWM35_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HAP9919017.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            complement(14309..15544)
                     /locus_tag="IWM35_001152"
     CDS             complement(14309..15544)
                     /locus_tag="IWM35_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAP9919018.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            complement(15541..16827)
                     /gene="sufD"
                     /locus_tag="IWM35_001153"
     CDS             complement(15541..16827)
                     /gene="sufD"
                     /locus_tag="IWM35_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HAP9919019.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            complement(16840..17613)
                     /gene="sufC"
                     /locus_tag="IWM35_001154"
     CDS             complement(16840..17613)
                     /gene="sufC"
                     /locus_tag="IWM35_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HAP9919020.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            complement(17810..18658)
                     /locus_tag="IWM35_001155"
     CDS             complement(17810..18658)
                     /locus_tag="IWM35_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP9919021.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            complement(18682..19368)
                     /locus_tag="IWM35_001156"
     CDS             complement(18682..19368)
                     /locus_tag="IWM35_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9919022.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            complement(19361..20395)
                     /locus_tag="IWM35_001157"
     CDS             complement(19361..20395)
                     /locus_tag="IWM35_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP9919023.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     gene            complement(20790..21128)
                     /locus_tag="IWM35_001158"
     CDS             complement(20790..21128)
                     /locus_tag="IWM35_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HAP9919024.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     gene            complement(21130..21486)
                     /locus_tag="IWM35_001159"
     CDS             complement(21130..21486)
                     /locus_tag="IWM35_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HAP9919025.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            complement(21492..22679)
                     /locus_tag="IWM35_001160"
     CDS             complement(21492..22679)
                     /locus_tag="IWM35_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAP9919026.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            complement(22870..23715)
                     /locus_tag="IWM35_001161"
     CDS             complement(22870..23715)
                     /locus_tag="IWM35_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP9919027.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            23949..24914
                     /locus_tag="IWM35_001162"
     CDS             23949..24914
                     /locus_tag="IWM35_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HAP9919028.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
     gene            25291..26334
                     /locus_tag="IWM35_001163"
     CDS             25291..26334
                     /locus_tag="IWM35_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HAP9919029.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
     gene            complement(26396..26860)
                     /locus_tag="IWM35_001164"
     CDS             complement(26396..26860)
                     /locus_tag="IWM35_001164"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HAP9919030.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQLLLLEGYLQN"
     gene            27106..28053
                     /locus_tag="IWM35_001165"
     CDS             27106..28053
                     /locus_tag="IWM35_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAP9919031.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            complement(28264..30336)
                     /locus_tag="IWM35_001166"
     CDS             complement(28264..30336)
                     /locus_tag="IWM35_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAP9919032.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
     gene            complement(30269..30646)
                     /locus_tag="IWM35_001167"
     CDS             complement(30269..30646)
                     /locus_tag="IWM35_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HAP9919033.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            complement(30775..31335)
                     /locus_tag="IWM35_001168"
     CDS             complement(30775..31335)
                     /locus_tag="IWM35_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP9919034.1"
                     /translation="MKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDIKAPSLY
                     NHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSNQAVYEL
                     LISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYLSLRFLG
                     YFTKEPTIDPEDSYFWMINNFIATLP"
     gene            31485..32777
                     /locus_tag="IWM35_001169"
     CDS             31485..32777
                     /locus_tag="IWM35_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAP9919035.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSIASGHSPSHRQ"
     gene            complement(32837..33955)
                     /locus_tag="IWM35_001170"
     CDS             complement(32837..33955)
                     /locus_tag="IWM35_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HAP9919036.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(33969..35390)
                     /gene="arcD"
                     /locus_tag="IWM35_001171"
     CDS             complement(33969..35390)
                     /gene="arcD"
                     /locus_tag="IWM35_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HAP9919037.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            complement(35636..36757)
                     /gene="mnmA"
                     /locus_tag="IWM35_001172"
     CDS             complement(35636..36757)
                     /gene="mnmA"
                     /locus_tag="IWM35_001172"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HAP9919038.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            37161..37760
                     /locus_tag="IWM35_001173"
     CDS             37161..37760
                     /locus_tag="IWM35_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphatase"
                     /protein_id="HAP9919039.1"
                     /translation="MNYFISDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            complement(37898..39184)
                     /gene="efmA"
                     /locus_tag="IWM35_001174"
     CDS             complement(37898..39184)
                     /gene="efmA"
                     /locus_tag="IWM35_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HAP9919040.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLTGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(39455..39799)
                     /locus_tag="IWM35_001175"
     CDS             complement(39455..39799)
                     /locus_tag="IWM35_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HAP9919041.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
     gene            complement(39936..41072)
                     /locus_tag="IWM35_001176"
     CDS             complement(39936..41072)
                     /locus_tag="IWM35_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAP9919042.1"
                     /translation="MKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGRHAH
                     EKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIEHPA
                     VLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLMPIK
                     EIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKRDGV
                     NIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLILDTL
                     EANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNIEPS
                     HVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
BASE COUNT        11872 a         8671 c         7199 g        13502 t
ORIGIN      
        1 tgatttaatt ctgcacccaa aaagatacac tcctatctct tatttaaagt tgctatatac
       61 cctcttattt tagcataccc ccttttataa aaagcaaaaa aaaaaagaaa gaaacttatt
      121 cttgctaata aactacgcga aaggagtcta acgttgtttg atataactta gactgatcgt
      181 taacttattt tttatatttc tacatagtta tctttagaac catttcagat acatatgaaa
      241 caattacata tgattttttc ataaaaaaac agtactgtct agtagtataa aatatacaaa
      301 tttcttaaaa attttttcac taaaatcact acctgaaaca gtctaaaaca cttatatatc
      361 aacgtttaat gaaactatct ccgtttcatt tcagatacaa ataaatatag tcgggctcca
      421 aatccgaaaa aaaatacccc cagtagctta tcctaagcta ctggagatgt ataagtacgg
      481 tcatataaca acggggtagg gtaaataaat gaaaaagatg gaagaatcat tattcaataa
      541 cttttttaaa tacttcgttg taatcagtta cctgtccgtt cttaaatacc acagaataat
      601 cttttttccc attttcaagt aaccgaagtg gaatgtcttt accaacttgc tctgtcactg
      661 cattactgat acctgttaga ttagctaata aatctgataa agattgagca tctgtagaaa
      721 tctctgtatc agtatttatc gtgattcctt tatcttcatt tacaagcttc caagtaggtg
      781 gtatagctgt gtatgtttcg atgaatgaat caattctttc atctatcgtc ttcccagtat
      841 ctacttctgt ctcattactc ttagatgaat cgattctttc atccatcgtc tgctccgtaa
      901 ctgcttctgt ctcattattc tcaccggaaa ctctcttcac acccaaactt atatattgtc
      961 catctttagt gtgcatagtt aaaattccca cgcttttact aataaattca ctataaccag
     1021 ttctttcaat agagacttca atgtatttca cactatcata ctcaaaagaa aaatagacat
     1081 gtaaaagcat tttgttgttt acatctacat aataatcctt tgactctact tgagaccttc
     1141 ctatacggaa tacagcttta ctgtctgata tctctatacc agcatcctta ttattaatct
     1201 gccaatctcc aagtagctct tctggcaagc tagtagtttc ttgtgaacac tttaccactt
     1261 cactggtaga ggtcgaaaca ttcacctccg cactgttaga agaattttcc aaggaatcag
     1321 gagccttagc acaaccaaca agtctttata tattgtgcag ccgatatttt cttcattcag
     1381 tacacccttg aaatatgcag tgttagataa agcagttcgt cctgatttgc tgaggcaccc
     1441 atctcttctt gaacgtactc cttgattttc aaggccgccc gatactcttt cggaaaacgc
     1501 tgcttcactg aagccagcaa aaaatcatcc ttctccggca actcttcctt cttaatcatt
     1561 cgggcaataa aaaactgtaa atgcgtcaca aatcttaagt aaagcatcga atctttatca
     1621 accttttttt cgggaaacag atatcctagc atagccaaga tatcctttag tgctcttacc
     1681 gagagcatgg tttcttccat atttgcttgt tcactttggg catttacaat gtgaaacgca
     1741 atgtttccag cctcctcttc tggcaactga acattcattt ttttgttgat caattggata
     1801 gcatagcagc cgatctcaaa ttcttttggg taatactttt gaatttcaat cagcaggcga
     1861 ttttgaaaaa caatgccttt ttgactgcgt tcaatggcat gcttcaagtg atcaaccaag
     1921 gtaaaaaata gacttttatt cagcttaaag tcaaacttgg cgttggcata atcgataatt
     1981 cgcttgacgc aaataattag atcatccgga gcagcctcaa acagtttaat gtaatccttt
     2041 tttagagcat cagaaagtga atctaattta aatacttttt ggattttgtc agccggaacc
     2101 aaatcgcctt tctttagttg aaatccaatc cctttcccca tcaaaattac ttctttgcct
     2161 tgcttatcta agcaaagaat taaactgttg tttaacgttt taattactct cattagattc
     2221 accgttctct ctaagctaag ccatttcttg cgattacttt ttgataccaa aagaagcttt
     2281 ctttttttat tcgttttaat tctttaagat tctcatcggt tcggtcaaca tatataaaac
     2341 cataacgctt attgaagcct tgatgggtac taacaacatc aatcgctgac caaggtaaat
     2401 aaccaaaaac atctacacca tctgttaaag ctgcttgaat agccagaata tgtttggcta
     2461 gataatcaat tcgatcctgg tcattaattc gtctatcttc ctcaactatg tcgggtgccc
     2521 catagccatt ttcagtaatt aaaataggta aatcatagcg atcgtacaat ttcctcaatg
     2581 ttaatcgtag tccagtaggg tcaactaccc agccatatgg cgttttatct acataaggat
     2641 tttcagctgc ccggtagaca ccttcttctc ccaacatgat ctgttgatcc cccgctctag
     2701 cggaaacatc tgatccgtca tttttgctgg cagcgatggt ggcagttgaa taataattaa
     2761 tcgcaataaa atcgggttta ccggaggata aaattgcctg atcctctgcc tcaattttag
     2821 gatataatcc acgatcattc aaatagtttt caaacaaata attgtatttt ccccgaactg
     2881 ctacatctaa gaaagaccag cctctcaatg tttcccaatt atgggctgca attgcatctt
     2941 ctggactgga ggtagcttga tacatagccg ttaaattgat tgctggaccg atttttcctt
     3001 taagattcaa ctgatgatac aagtgaatta ctctagcttg agctaataaa acatggtgat
     3061 taatttgaaa gagttctttt tttgaaggca gctctccact tggtggcaaa ccaatcgctc
     3121 caggatgaag gatcatcgtg ttttgctcat taattgtcag ccaatagttc accttgtcac
     3181 catactcagt aagcaatacc tgtacatagt ccacaaaata atcgatagtt gctcgattta
     3241 accaaccgcc ctgctcctct agaaaataag gtaaatcgaa atggtacatg gtgacaatcg
     3301 gttcgattcc ctgattcagg cattcatcaa ttacttgatg ataaaaagaa attccctttt
     3361 gattggtttt attattttca atatccataa ttcgcgtcca agcgatggaa aagcgaaaag
     3421 cttttaagcc taattcttta aacagctgta tatcttcttt atagtgatgg taaaaatccg
     3481 aggcaacttc aaaattagct accccttctg tatgctgata cttgtcaata atagacggtt
     3541 gtttcccatc ctcctgatag gcaccctctg cctggtaggc actaatggag gcaccccata
     3601 aaaacttctc tggaaatgct gaaagctgtt tgtattgcac aaaaatcctc tccttaacta
     3661 atttgcatca atggttcgtg ttcctttaag cttccgcttg cttcactaat tcctgcgtaa
     3721 ttttcactgt tggtcactac cacaatcact gtatcatcgt agccagcgtt tctaatggca
     3781 gcgaaatcgg cgttgattaa taattgtcct gctgacacct gatctcctgc ttttacaaat
     3841 acttcaaagg gttccccttt taaattcacg gtgtcgatcc ccacatggat caataattca
     3901 ataccttctt tagaacgtag accaatcgca tgtttagatt ctaaaatagt aatgacttct
     3961 ccctcaacag gtgaataaat gctgttttct tctggatgaa tggctacgcc ctttcccaat
     4021 agtcccttag aaaagacagc atcgttcact tcagaaatag gacacacggt tccttttacc
     4081 ggattttcaa caatgactgc ttcggctatt gccgtttgct ctgattcatc agaagtatcg
     4141 tcaaatccaa ttagataagc aatgataaag gttgcaacaa aggcgattac acacgtaata
     4201 attgcgtgaa caatattcat tggattttct ccaataaagg ctggtaaggc tgctaagcca
     4261 ggggaaacaa aggcatatct gactaaaccg ctcaagccag catacaaacc agcaattgca
     4321 ccaccaatca tggaagcaac taatggtttt ttcagcttca aggttactcc atagagtgaa
     4381 ggctcagtaa ttcccattaa agcagtgatc ccagtggaca cagctagctg cttcaactct
     4441 ttgtttttag ttttcgcggc tacagcaaag gtagcacctg attgagcaat attagaagcc
     4501 agcattcctg gcccattgat catttcagaa cccgtactgc tcaattgcat agtagcgatt
     4561 ggcgtcattg cccaagctgt acccgtaacc accaaaaatg gctgcaaacc acccatcaaa
     4621 aatggaatta accaagctgc tttgccgtta atccattcag cccccattgc tataagatcg
     4681 ttcagcaacg ttccaaacgg tccaatgaca accaacgcta aaggccccat aattaaaaat
     4741 accagcatcg gcttagtaaa gaatttaata aacgaaggcg aaattcgatc cgcaaagggc
     4801 tctacatagg acatgatcca aacagtaaag ataatcggta aaactgatcc cgcataatct
     4861 gcagctaata ccggaattcc tagaaaagaa acggcatcgc cagtagaaaa aagctgtgaa
     4921 atatttggat gaagcaaaat gccagcaaca gtaatcgcca aaattgggtt gacatcaaac
     4981 tttatggctg ccccataggc taatagtact ggcataaaat aaaaggctgc atcagcaata
     5041 gtattcaaaa tgatgtaagt ttgactactg tctgatagta caccagtcaa agtcaagacg
     5101 gccaaaatgg cttttaccat cccggcacct gttatagctg gaatcatcgg tgtaaaggtg
     5161 gtagaaatga cactaatcaa tcttgaaata atactttctt gattgtctga agaggggccc
     5221 tttttaattg aagttcccac ttccttttga atttccttga aggctgtttg gacttcattg
     5281 ccaatcacca cttggtattg tccaccgcta tccaccacac taattacacc aagtgttttt
     5341 tctaaggctt tcgtatcggc tttcctttta tctttgagtt caaaacgcag tctggtggca
     5401 caatgggtta agctgaccac attttctttt cccccaacct gttgcaatac atttcttcct
     5461 acttctctgt aatccattat tctctctcct taaagcagta aaaaagacca agtcaaacca
     5521 cgccccaatc aacattgaag tgttagtttg tcttggtctt gcctgcataa tcagtaacat
     5581 gccaataacg ttattttgct gtaatccaat aatagcacat tatgatagcg caatcaacag
     5641 ggaatttaat tttttatctt ttcgtttgac cgtatagggt aatataacag ttagatcaca
     5701 tcaactaggg tatatctgcg tatacttact gctaaaataa gtatatctaa tattaactat
     5761 accagtaaaa ccattcctac ataaccagcg ggaaattccc aaaatcgtga aagcatcaaa
     5821 aatgcatata acggatcaca caacttcaac aattctgctc ttgggaaaaa ttgatgaatt
     5881 tggtgagtaa aggatggaat aaacagcatt ccaacaatat aggcaacaat agtgatactt
     5941 gtttttttgg taaaaatcag taagcaatag ccaatttcaa tcattccgag atataaaggt
     6001 atcgatagaa tgatttgggc tagtattctt aaaaattaac tagcaaatgt ttaaactgat
     6061 ttatttctaa tctacatgta gtaagtattt caattccacc aattatcaat aaaaatacca
     6121 aacatatcca taataatttt ttctttttca tttcttttat attgttataa cagtgaataa
     6181 cataaaaatt cccgacaatc attgtaatat ttttttgtca cctgtattag tatttaattt
     6241 ataggaaact ataaaaactc cctagtgaat ttacaccttt gggagttcct gtttggacgg
     6301 tatgcataat acatttaaat caatttaaca ttaacacctc agcatatggt atctccacat
     6361 atatacggtc tttctttgga tagtactcaa tatttatgtt gatagcactt ccattgtcta
     6421 atatagcttt agcccggtat gtgttatctc cattttcatg agtaatcgtc acatcatcac
     6481 atgatcgatt taagttgtac tgttcttgta aaatctgtgt taccaatggt tttgatgact
     6541 tctctaatcc agatgtacct gtcatttttg aaaaaataat acctataata gccaagacaa
     6601 caatacctat aatgacaaac ttcttgttca tatttacttt attcaacttt ttatctccta
     6661 tctccaaaat aatttgctct tatccctctt atcagtacat tgacatccct aaaagtacac
     6721 caaagattgc tccagctacc cctgctatca tcaaaatcgt acccattttc tcatcttttt
     6781 ctcgatataa gtagcccata ataacagcaa cagcaccaaa tacaacagga ataaatgcta
     6841 acgatactac catagaaatc caaccaataa tcaaatacag cttccctgtc tcttgtccat
     6901 gtacgatttg ttccttcatt tcattaattg tctgctcagt acttttcttt actgtgtctg
     6961 ttccggttgt ctcatctgtt tcttgtagtg ctccacaaaa ctggcaatac ttactttcat
     7021 ctgttatttc tttgccacat tttctacaat acatgtcttc tcctcctaaa atcattcaat
     7081 tgcttattag ttaactgttt actttattaa atacatcaga aaacagctca ttcattggta
     7141 cacttaatat ctcagaaagc atcttcattt gatagggttt aggaatcgtt ctccctgttt
     7201 gccacgaact tacacattgc tgtgtaacat tcattttttt tgctaactcc gtttgagaaa
     7261 gtcctttttc ttttctgtat cggctgagat tacttacttt tttcatgatt ctcccctcct
     7321 gttttattat atacaacaat atgatgtatt tcaactcata aaatataaaa aacttcaaat
     7381 accattacaa atacaacaac ttgttgtact atactgagat gggggtgtat taatgaaaca
     7441 gaaacaatat gcaaaggtca ttagagaaaa gcgaaaagct ctcgggtgga cacaaaaaga
     7501 attagctgaa aaaatcttct ctacacaaca ggcagtagca agatgggaaa attctgtaac
     7561 agagcctaac ctcgatagct taacagctct ttctagagcc ctaggaaccc ctgtaagcca
     7621 ttttctagac aatgttgtcg tagattatga ggaagagttt ttggctctct atcgttctct
     7681 tagcactgaa gatgcagttc gaacaattga ctatatgaag ctattaaaaa ggcaagaaaa
     7741 tgaacgcaat cagctattga aagattgaaa aataaataac ccaccgaact ttaacggtgg
     7801 gtgtattgag atgaaataag attaagagct atggataacc tgtctagttt aatacaattg
     7861 ggtcttttct cttccttact ctccgtccat catctcttgt tacacttgta aattgataca
     7921 gaggaactat cccagctcct gataatatca attcttttat tgtttcatct atccctgccc
     7981 ctttcgctaa atatattaaa aaactactac ttgctttcac attgtatagt ttcttaatat
     8041 ccttagaagc tcgttcaaga gtgaatcctt gtttaacaaa gtagcctctg ttttttatta
     8101 aagcattttt cacgctgtta tccccaacat tacgtaggcg ttcacaaaca gtgttaataa
     8161 gttctttctc tgtcattaga tatgtccctt gccacgataa gccctgtaaa acttcatgtg
     8221 cttcatctaa aatcaattgt ttttccttgc tgagttctga tcgggaattt tgaggaaata
     8281 ctcttttagc ctctttttcg ttaaaacata tcttaaccca ttcataagaa aaatattgat
     8341 atgtaaaatg aattttttcc agaagtggaa ggatatcttc attcaatttt tgaatcacag
     8401 aagttgcatt aaaaacgtct aatcgataga cattgcttct cttatgatta aagtttccta
     8461 acgctttctc ttcctttaac ctatttaggt attcgagcat cttcaaagga acatcttcat
     8521 catctaattt ttttatcaat cctataaaag tcataagtga tagtaactta ttacatttat
     8581 ctatgtcata agttttacca tagtaagaac tcattttttt agctaattgt tcccccgaca
     8641 aaatacaagt cacactttcc ttactatcat caaaaaaatt aacatcagaa agcaagacat
     8701 catagacacg tcgcatttcc tctagataat tccttattgt cctgaaaagt ttgtctttca
     8761 ttgattcaag agtgtcaaaa agtacttttg attcctcttt caattttcga aaatgtgtag
     8821 gatacaagtc taggttctct aatagccaat taaatgtttc tacccgactg tctttcttta
     8881 aaattagcat cacaactcca ataaaatttt cactgatatc acaattactg gaatgacaat
     8941 aaaaaatgga atgcccagtt ttactggtaa aaatactagc agagggatta tgatcgttgt
     9001 gaaacagaca tcttaagttt ttacttgact taagtaattt attcatatca atggttaaca
     9061 atctttcata aatctcatta aaagacatgt cggaggaatc aaacaggcag tctttaaacc
     9121 actgtttcat ttcttctttg tcgtcatttt ttatcaactt aacaaaatcg gtttcattga
     9181 ctagttcata ctctctccct gaatcacttt gacaattttt agaacatgtc atggtaaaat
     9241 caatcggaag ctcagtaata tctaacatgt tgtcttcatt aattattatc actgcatctg
     9301 acttatatcc tccaaaaaac attctattcg aagcatttac attgtcatca cacccgggga
     9361 tgttcttttt caagtattcg taaacagcaa ccattttacc atagtcagta attgcttctt
     9421 ttaatagaaa gacaatcttg tatttttcta gattttcaga gtaccgaata cttttttgta
     9481 tcgcaaacgc agaattatga actaaattga tagataaaac cttttctacg cttaagtatc
     9541 tactatcatc tttactcaaa tctactacct tccccttctc atctttaaca gtgttatcga
     9601 catccagaat aattactctg gtttccacaa aactctcctt gtcattgcct ttattctttg
     9661 tcttgcttgg aataaatgat tgtgatgaat ttagtatctc tacaaactca tctcttgtaa
     9721 cttctctata gttatctaaa cacctatctc ttacatcagt tttttctttt tttgtaactg
     9781 gcttctctaa taatttttct tgatctacat aaattggaat attaatcatt gatgtttcca
     9841 tttgtgtttc ctccttataa aattctgtta aagactaaaa catacagata ataaaaacaa
     9901 actctttatt actatatgtt ttagtcttat ttgtttactc aacctttata ccgttccatg
     9961 aaactatcaa tatcagattt tttaaatcta atgaccgtat caatttttat aacaggtaac
    10021 ccgtattgag aaacatattt tctaatcgtt cctactgata cacctagata ttcacttgcc
    10081 tgctgatagt ccatatactc tagagtgact ccagaaataa tgtcttctag cataggcttt
    10141 agcactgtaa ttatctcttt cacttgttgt tcaacttgta tagaaacaat gctatcaatg
    10201 atttgtagag acatatttcc ccctcctttc gtgattgctt gaacaaattt tctaagaatt
    10261 atcttcgtat tttttgctca aaataaaagt attttgttat taaccgatta atcaaacaaa
    10321 aaatgattac cagttcatcg acatacacat aatacctcga taaaaaaggg gtgtcaacag
    10381 ctttgtaaaa tttatttgca acaccccttt tttgaggtat actaatttta ggaggtgtgg
    10441 tcaatatgat aaaacgtcca ccaataaatt atttagaacg aaaaaagata ctcggaacaa
    10501 aaataaaagc tataagaaag agtaaaaaat taacacaacc agcatttggt cttatgatta
    10561 acaacggtca attaattgac aaaaaaacta tctacgaatg ggagaaggga acctatttac
    10621 caataccaga gcgattatcc cgaattgctg atttgggaaa tatgagtata gaggagttag
    10681 tttgtggcaa tgtagaagaa tatattttgg gaattattct ttatagagat agcattgtac
    10741 tagatggtat aacatttcca gataaaaacc tgttccagca tcttcgtcaa cagttcccac
    10801 cagtacactc taatttagat acatggctgg atagatattc caaattagaa ccagagatgc
    10861 aagaatttat tgctaacaag acttgtaata aagttaaaaa tgaaaaaatt agtcttttca
    10921 atatcttaaa aatagaagaa ttattcatca atgctattgt agaagaattt gacaataaca
    10981 ttcttttcct cactagcagt atagaagaac tactagaaag aatggtagat gaatggctac
    11041 ctattcaatt gaaagacatg tcttatcccg aagaagctgt gagagaaatt actgataata
    11101 taaacaagct agaacaaacg atttcatcaa ttgggaaaaa atacactaag aaaaaaatga
    11161 agggaggtga taccatctag tggtaagtac tccttgtaaa ctaagaggag atgatttaat
    11221 ggcaaaaata tcaaagtaca aaaagaaaaa cggtcaagtg gcgtggatgt ataaaggaca
    11281 cattgccacc gatccacgaa ctggtgaaaa aataaatact acgagaagag gttttagtag
    11341 caaacaagaa gctcaaaaag attatgacga gtacagacat caaatattat acggtgttaa
    11401 aaagaaagca cctgatatga catttgaaga cctgtataat gaatggataa ctcatcagcg
    11461 taccagtgtt aaagcttcaa caatcgcaat ctcagtaaga tacgcaaata atcaaatact
    11521 tccagcattt ggtaagctta aactatcgaa tatctctgtc ccatattgtc aaaaagtggt
    11581 agatgaatgg cacagtaaat atgaaagtta cgattatatg agaaaacaga ctgcacaaat
    11641 tttacgctac ggggttgcaa tgcaatatat tgacaataat cctatggaaa aaacacttct
    11701 tccacgaaaa aaagagtatg agaaaaatcg taaattttac tcaaaagagg agcttaataa
    11761 tttactggat gcttttaaag attttggtaa catgaaacaa tacgccttct tcagattatt
    11821 ggcatacaca ggtatgagaa agagtgaagt actcgcttta cagtggaaag acatagacac
    11881 gtttaataaa gaactacatg tcaataaaac tcttgctgta gatgaatttg gtaaagttat
    11941 aattcaatct ccgaaaacaa gagcttctag acgagttatc tctttagata ctgagactct
    12001 ttctatcctc aacaactgga aattacagca aaaagaagag tatttaaaac ttggatacaa
    12061 tactagctct aaagagcaac atgtttttac aacagttaaa aacactctat acattccaaa
    12121 cactgttaat gattggttaa gatatatttt gaaaaaatat aacttgcctc gtattacacc
    12181 tcatgggttt agacatacac atgctagttt attattagaa gcaggagaat ctgtcaaggt
    12241 agttcaacaa cgattagggc atgaaaatag taaagtgaca ttagacatct atgcacatat
    12301 tacaaataat gcacctaaga aaacaggtca agactttgct gatatgatgg ctcatcagta
    12361 aaagagctgg tcaaaatctg gtcatttcta gtatacaggc aaaacaaaaa agctagaacc
    12421 cttatatatc aagggttcta gctcctataa caggtttacc caacactgcc ttccatctca
    12481 taactgatca atcgattgag ttcgactgca tattccattg gaagttcttt ggtgaatggt
    12541 tcaacaaagc ccatgacgat catttcagtt gcttcagatt cggtcaggcc tcggctcatc
    12601 agatagtaga gttgttcttc tgagattttt gagactttgg cttcgtgttc taaagagact
    12661 tggctgttgt ggatctcatt gaatgggatc gtatcggatt tcgaccattc gtccatgatg
    12721 atcgtgtcac attcgatatg agagatcgaa cccgcactgt ttttggcaaa ggttacttgt
    12781 cctcgatagt ttacttctcc gccgtctttt gaaattgatt tagagacgat agagctagat
    12841 gtatttggtg cattgtggat catttttgca cccgtatctt ggatttggtt tgctcctgca
    12901 aaggcgatgg agagcatggt gcctcgtgca ccttttccat ctaagtaaac acttgggtat
    12961 ttcatcgttg tttttgcacc taggtttccg tcgatccatt caacggttgc gccttcgtat
    13021 gcttttgctc gtttggttac taagttgtac acattgtctg accagttttg gatcgttgtg
    13081 taacggcaat acgcatcttt tcttgtaaag atttctacga tggcggcatg caggctgtta
    13141 cttgaataag taggtgccgt acaaccttcg acatagtgga tgcttgctcc ttcgtcaaca
    13201 atgatcagcg tacgttcgaa ttgccccgtg ttttccgcat tgatccggaa gtacgtttgt
    13261 aatggaacat cgactttgac gccttttggc acgtaaataa aggtcccacc tgaccatacg
    13321 gctgaattca gcgctgctaa tttgttgtcg gttggcggta caagttttga aaagtactct
    13381 ttgaataatt cagggtattc tttcaaagca gagtcagtgt cagtaaagac gattcctaac
    13441 ttttcgaatt catctttcat attatggtac acgacttctg attcatattg tgcggaagca
    13501 ccggctagat aagctcgttc agcttctggt ataccaatct tttcaaatgt atctttgatt
    13561 ttttctggta cgtcttccca gtcacgagcg ggtttgtcac ttggtttttg gtaatatttg
    13621 atggcatcga aatcgatgtc tgaaaggtcg gggccccatg tttgcattgg cattttgtta
    13681 aatgtttcta gagatttcag acggaattca agcatccatt ctggttcgcc tttgattctt
    13741 gacatctcac ggatcacttc ttctgtcaag ccttctcctg tactgaaaac cggttcgaca
    13801 tcatcgtgaa acccgaattt atattcttct aattctggta cgcccatgtt ctcactcctc
    13861 ttccttgtgg taatgatgga aagctgcggt accatcgttt tccattgctt ttcctagtgc
    13921 tttccaggca agtgtcgcac atttgatccg agcgggaaat ttagctacac caccaagcat
    13981 agcagcatcg cctaattggt cgggatcttt gacttctttt ccttggacaa gctgtagaaa
    14041 gtctacagtt aaatgttctg cttcttctat tgattttccg atgacggcgt ctgtcatcat
    14101 cgaggcactt gccgtactga tggagcagcc actgccgtca aaggcgatat ttttgatgat
    14161 tccgtctttt acatcgactt gcaattcgat cacatctcca catgtggggt tgttcaattc
    14221 gatcttgctt gtcgagtcag tcaagcttcc acgatgatgc ggatgggaag aatgatccaa
    14281 gatcacttga cggtataaat tgtctaattt agatagtccc atgttggaaa aactcctttg
    14341 ttgccatgat tgcttcaact aaacggtcgg cgtcttgcgt tgtgttgtag aaataaaagc
    14401 tcgcgcgagc agtcgctgat acttcaaggt acttcaatag cggttgcgca caatgatgac
    14461 ctgctctgac ggctacccct tccatgtcta aagctgtcgc gacatcatgg ggatgtaacc
    14521 catccaaatt gaatgcaacg actcctgtcc gaaaattcgg gtcttgtgga ccataaacag
    14581 tcaatccatc aattgcttgt aattttggca acacataagc ggctagttct tcttcatatc
    14641 gatggatatt gtccataccg agtttttgca aataatcgat tgcatggcct aatgcgatag
    14701 cgccggcgat atttggtgtc cctgcttcga atttccaagg tagttctttc catgtactgt
    14761 cgtataattc gacaaaatcg atcatttcgc caccaaattc aacaggttcc atttcttcta
    14821 accattttcg tttaccatat aaaacaccaa tacctgttgg tccgcacatt ttgtgtccgc
    14881 tgaaagcaaa gaaatcacag tctagtgctt ggacatcaat ggccatgtgc ggggcagatt
    14941 gagcaccatc tactacgatg accccgccgt tttcatggac aagttcagct aattcttgaa
    15001 caggattgaa gacacctaag acattggata catgagcaat ggagacgatt ttggttttgt
    15061 ccgtaatctt tttcgctgca tcctccatat ccaggaagcc ttcttctgtt agttcgatat
    15121 atttcaagac agcacctgtt cgttttgcca actgctgcca cgggataaca ttagaatgat
    15181 gttccatgta agagagcact atctcgtctc ctgctttgat atagcggtcg ccgaaggttc
    15241 tggctaccca attcaagctt gtcgtcgtac cacgagtaaa cagcacttct gctgtttctt
    15301 ttgcatggat gaacgcccgt actttttctc ttgcttgttc ataatcatgg gtcgcgcgtt
    15361 ctgctaacgt atggacaccg cgatgaacat tggcgttatc gtgttcgtaa tagtacgcta
    15421 atgcctggag tacttgtttt ggcttttggg tcgtcgctgc attatccaga taaactaaag
    15481 gttcatcatt gacgtcttga aataagatag gaaaatcatt gcgtagcttt ttagcatcga
    15541 tcatccattc aacttccctt cgattacttc gactaattct ttccgaactt gttctacagg
    15601 gattgctgtt aagacagaac cgaggaatcc tcgaatcact aagcgctctg cttcttcttt
    15661 atgcaatcct ctactcatca gatagtacat ttcttctgga tcaactcgtc ctacactggc
    15721 agcatgtcca gcagttactt cgttttcatc gatcaaaaga ataggattcg catcaccacg
    15781 tgctttatca gaaagcatca agacacgact ttcttgttgg gcatctgcac cttttgcccc
    15841 tttcaagatg tggccgatac cattaaaggt caatgttcct ttttctcgga tcactccgtg
    15901 ttgcaaaata tgcccgatcg tatgaggggc tttattggtt actcgtgtat cgattccttg
    15961 gatttgtctg cccgagctga ttgccacgat tttgacttct gcgtgtgcac cttctcctgc
    16021 taaatctgag tcaaagtcag cgatcacatg accgtcattc atgacaccaa ttgcccagtc
    16081 gacagaagca tctcgtaaaa tgtgcccacg gcgattcata taagaagtaa tattttctcc
    16141 taattgatct acggctgaat atttgatttt tgaaccagct ttagcgatga cttccacgat
    16201 gatattacct gatgattttg ctacttgttc tttggttgtc tggaaacgtt ccagataaga
    16261 aaattcacta tgttcatctg ccacgatcaa cacatgcttg aagaagtgct catcggatgc
    16321 gccatcttga atgaataagg actcgagtgg ttcatcgatc acgacatttt taggcacata
    16381 caggaaaata ccgctgttca taaaagctac atggagtgca gtcaattggt cttcgttcat
    16441 gtttactgct tttttcatat aatattcttg gactagttct ggatattcga tcattgcagt
    16501 gaacaagtcg gtaaaaatga ctcctttttc tgctaattcc acaggcagtt gttcaaaaat
    16561 cgtccatgaa ccttgttgga caagcacagg attgtctttc attgcatcaa atgccggaat
    16621 cgtgcctgtt tcactgatgg tttgtgtttc tttcacatca aataacgacc agcgatggaa
    16681 tttcacccga tcgatatgcg gcaatggcag ttcatctgct ttcgctagtg cgtcttgacg
    16741 gaaggttctc atccaatcag gttcgccttt acttaaagaa aaatcattta ccgcgtcaag
    16801 ataatcgagc cagttttttt ctttcattct tgctccccct tacgcttctt cttctttata
    16861 gtcgattcct aattctttac tgataccagc gtagccttct gcttccagac gtttagctag
    16921 gtcagcattc cctgtcatga ctactcggcc ttccatcatg atgtgaacga catcaggtgt
    16981 gatatagttc aataatcgtt gatagtgggt gatgatcaat gcaccgaaat tgtctccgcg
    17041 catttcattt actcctttag caacgacttt caaagcatcg atatccagac ctgaatcgat
    17101 ttcgtctaaa attgcaaatg taggttctaa catcaacaat tgcaggatct cattccgttt
    17161 cttttctccg ccagaaaaac cttcgttcag ataacgttca gccatttctt ctggcatatt
    17221 tagtaaagcc attttttcat ctaacttttt caaaaatgcc ataacagaaa ttttgtcgtt
    17281 ttcatcacgt ttcgcattga tcgctgcacg catgaattcc gcattcgtga tacctggtat
    17341 ctcacttgga tattgcatag ctaaaaataa gccaagtcgc gcacgttcat ctacttccaa
    17401 gtctaaaaca tttttcccgt caaacaaaat ttcgccttct gttacttcat agtttggatt
    17461 gcccatgata gcggcagata aggtagattt tcctgtgccg ttaggtccca tgatggcatg
    17521 gatttctccg gttttcattg ttaggtttac gcctttaagg atttcttttt cctcgatcga
    17581 cacatgtaga ttttttattt ctaagacaga catgcattat acccccaaat tctctttcgt
    17641 tctcactcat tttagactaa ttgagaatga agcgcaatga ccttccttac taaaatcaaa
    17701 tatcttttat ttattattaa acaattttga tttctaaatt agaataggta taaaaaagat
    17761 gagaaataag ccattacaga cttatttctc atcttttttg ttccatacct tattttgcat
    17821 cagcctttac tggtttaaca gagccgcccc atttgtctgt gatccattct tggacttctt
    17881 tgcggtgaag cacttcaatc agttttttga ttttgtcact gtcttcatca cctttacgag
    17941 tcgcaataat attcacgtaa ggagagttgt ccttttcaag caatacggca tcttctaatg
    18001 gattcaaccc tgcgccgtaa gcgaagtttg cgttgattgc aactaggtca ccttcatcat
    18061 tttggtacgt agaagctaaa agtgatgggt cgatcgtatg gttgaacttc aagttcttag
    18121 ggttttcttc aatatcatcg aatgttgctg tttccagatc tactccatct ttgactttga
    18181 ctaagccagc atcttgtaga attgtgatga tgcgtcccca gtctgattca gaatttgaag
    18241 taatgattgt tgcaccatct tttaattcgg aaacatcttt gactttttta gagtacaagc
    18301 ccattggttc gatgtggaca gcaccggcat tgacaaagtc atatcctttt tctttgattt
    18361 ctttgttcaa ataaggaata tgctggaagt aattggcatc aatgtcgcct tcttccaaag
    18421 attggttcgg taatacatag tcatcgaatt tgacgatttc aagatcgatt ccttcatctt
    18481 tcaataatgg tttcacatgt tccaaaatct ctgcgtgcgg tgtcggtgaa gcgccgactt
    18541 tcaatgtcgt gtcgtctgct ttatttgctg aatctttgct gtcgctgttg ttaccgcatg
    18601 ctgctaatcc tacagtcagc aaaagcgctg ctgcaaatcc aaaaattttc tttttcatat
    18661 cctacatccc cctatatcct attatctttt gtctgttttc ttagttaaga aatcaccgat
    18721 tccttgaacg ataaatacga ttagtaaaat aattaaagtc gcaacgaatg tcactgttag
    18781 attaccacgc tggtagcctt cctgccaagc aagacctcca agtccacctg cgccgatcgc
    18841 gccggccatc gcagtaaatc cgatcatcga gatcgttgta actgttaatc cagaaatcaa
    18901 tgcgggaata ctttcaggta tcaagacctt ccagatgatt tcccagtaac ttgctcccat
    18961 tgcatctgct gcttccagta caccagggct gacttcacga aaagcaatct ctactagacg
    19021 ggcataaaat ggtgctgcgg ataaaacaag agctggaatg gctgcttgcg cacctagcat
    19081 agtgcctacg attgctttag taaatggaat gatcaaaacc atcaggatca taaatggcgt
    19141 tgaccggaaa atattactga tgatcgatac gattccataa atgattcgag catacggttt
    19201 gtttttgcgt ccaatagagt aaaggatcaa tcccaaaact aatccgatga tcgtcactaa
    19261 gaccattgag attaatgtca tatatagtgt atcaaggata gcctgattca tcgtttccgg
    19321 gttgatttgt ttaaaatcta gatatgtttc tgaaaagctt ttatccacga tggatcacct
    19381 caattcctac tccttgttct ttgatgaaca cttctgcttg atctaattct ttggtttcac
    19441 cagtcatcaa gacagtcaat gaaccaaagg aaccttcttt cacttgtttg atctttccat
    19501 acacaacgtt gacatcgatg ttatagcgtc gaatcgcttg ggaaatgact ggctcgttgg
    19561 catttctttc gctaaaggtc aaagttgcca aacggccgct tggattttct ttgatgagtt
    19621 cagctaatag ttctgtcgaa tcagcttctg gttcgatgtc ttgttgtaca aagcgtttgg
    19681 ttacttcttt tttcggttgt ctgaagacag tcaatacgtc tccttcttct acgactttcc
    19741 ccaactccat cacagcgact ttgttgcaga ttttgcggat cacattcatt tcatgtgtga
    19801 tcaagacgat ggtcaaattc aatttcttat tgatcgctaa caataagtct aatacttctt
    19861 ctgttgtttg cgggtccaat gcgctagtag cctcatcaca aagcaggatc tcaggatcat
    19921 ttgccaatgc tcgtgcgata ccgacgcgtt gtttctgtcc gccggaaagt tgtgaaggat
    19981 atgcttgtcc gcgtccttct aacccgacta aacgcaacag ttcttctgcc cgttctttac
    20041 gtttgctctt tggtacgcca gctaattcca gcggcagctg gatattttca agtacagttc
    20101 ttgaccataa caggttgaaa tgctggaaga tcataccgat tttctttcgg aatgtacgta
    20161 attctttgtt ttttaatttt gtaatatctt gatcgtttac gatcacttcg ccgctcgtgg
    20221 gaagctcaag tccattcagg agacggacta atgtactttt tcctgcgcca gaatagccga
    20281 cgattccata aatatcccct ttttcaacgt ttagtgaaac gtcattgacg gcttggatgt
    20341 ttccgttttt cccattgaat gtctttttga cattcgttag ttggattaat gacatgccat
    20401 tcacatcttt ctcttatgat ttttcaaaca aaaaagcctt cgccctcttg ccttaagcaa
    20461 aaggacgaaa gcttgaactt ccgtgttacc accttgattt accgttactt cgcaataaca
    20521 gcctcttcca gtacttggac ttagcttctt tccgtatact gtcgcgctgt aacgggcact
    20581 cccgtagtaa gtttgcttac gctcgcttac tctgctctaa gaccatcttc catctcattt
    20641 tcgttaccca ttttcagcaa acagggctct cttcaaacgt ccgtgacatg tactcttctt
    20701 ttcaaagctt tctcgtttca tataggattg cttatgatta tagcaactgg aagaatcagt
    20761 gtcaactttt ttcaagtgat tttctgattt tataattcgt caaattcttt tggatcaaca
    20821 tctttgaaac ttaagatcca cgcattcatt tcatctgaat cattcaagat attgatatct
    20881 tcaccgattt tatcattaac agaagaaact gtacccgtta gcgggctggc aaattcattg
    20941 accgcttttt ccgcttcaac ttcgatcaga ggttcatctt tttttagcga ttgtcctgct
    21001 tttggcagat tggcaaacgt gatgtctcca agttcttctt gcgcttcatt tgtcaaacca
    21061 acacagtatt cttttccatt aaataagatc caaaggttgt cctttttctt caaatgcttg
    21121 cctgccattt tatatcagtt ctccttcata aattttttca ttgaatccaa ttgcaacaag
    21181 ctggtcctct cgtaagatca gtggtcgctt gatcagcata ccatctgtcg ataagacttg
    21241 gctcgctttt gaagccgtaa actcgcctac ttgatctttc agtccaagtt cccgatattt
    21301 catcccgctt gtgttgaaca ggcgacggat tggtacttga ctcgtttcta gccaagtctc
    21361 aatcgttgag gccttcggta cttctttgat catatcaacg gtttgataac taacgccatg
    21421 ctggtctaac cacgctttgg cttttttgca tgttgaacat ttcgggtacc agtaaaaggt
    21481 gtacaatttt cttacctcct tgaagatctt gcaggtgctt cattttggta tctcatcgat
    21541 aatatcaacc cgataccgat catgttccct aaaatcgagg atcctccctg actaatgaaa
    21601 ggcaatggga tccctgttaa aggaagtaat ccgatatttg ctccaatatt ctcaaagaca
    21661 tggaacagca gcatcatgat caaaccgctt gcgatatagg cgtaaaattc attattcgta
    21721 tcgaaacaca cccgaatcat acgatagatt aaaataaaat acaagaaaat gacgaatgca
    21781 ccaccgataa agccgaagtt ttcaccgata acggtaaaaa tcatatcgga ctcacgaaca
    21841 ggcacataga tattgcttac gttgaatcct ttaccaaaca agccaccggt accaattgcc
    21901 aaaagtcctt gggccggctg ataactcatt cctgacgtat catggaaagg gtccagccat
    21961 gaatcaatac gtgcaaactg ataagatttg aatccaactt tataaagcaa gtctcttccg
    22021 ccttcagtcg taaccaagaa gatcgttcct gctccaacga ctactgccaa gattactaca
    22081 ggtgcgatga tgcgccaaga gattccagac atcaggaaaa tcccgccaaa gatcgcaaga
    22141 aagacaagca tcgttccaaa gtcatcttgg gccatgatca atgctaaaac cggtattgtc
    22201 acgatcaaga gcttcccaat cagccaaaag tccgatttca tcgtccgttc ttttacttgc
    22261 gtattatgtt tagttacgat cagtgccatc atcaagatgt acgcaatctt catcaactct
    22321 gctggctgga aagtaaacgc accgaatcga aaccagtttt tagaccctgt tgaagcagct
    22381 aaattctgat catagaattt gagcaagagt cccattacca ctaaccccaa accataaagg
    22441 taaggagtca gtttccagat ccatttggaa ttcaaatgca tgatgacaac gattgctgcc
    22501 gctccaacta aataccagac aacttgcatc cctacgcctt tcaagactgc tggattgttc
    22561 ggatcatggc taagtgctac atataatgag agcagaccaa tcaaacataa cagaaagact
    22621 ggtaaaatca cgccataatc aatacgatta tcgttcgaag cttttaattt tttttccatg
    22681 tttcatccct tttcttaaaa accatactgc tgattattat agcggtccag ttagataatg
    22741 gaaagtcttt ccatagacat ttgggaaaaa ttaactttcc gtttgtgcag ttgcttgagc
    22801 aaatgcagta caaaagccta ctgcttatct ttagtgtatc atagaacttt ttttgataca
    22861 tgcaattatc taccagctga gattctcctc gctgtgagaa gtttctgtgc tttcattgta
    22921 tcgaaggatc tcttcggcaa tctctagcag ctgatcagaa accagattgg ttcgttggat
    22981 gaaataaaaa tatcgatgga aattcaacgg agtcattggc acattttctg gaacagagcg
    23041 cttagacaaa atcgagcgac cgattccttc cttcagcaac gcaacaatta catcgttact
    23101 tttcacgatc atcttcttat cttggatatt catctcttca aaataacgct gcgtgtaatg
    23161 aaaaacacca gagtttgctt ctcttacaag ccacaatttg ctttttgggt ccccagcgat
    23221 gactagctgg tcttccatca aagagtatct ttgtacgccg gaagtctcta atggcttttc
    23281 gataaaacca aaatcggatt catgtttcgc tacttgttct aatgattcat gagaattcgt
    23341 gatttcgatc tcaaaatgca ctttaggaaa ccttttgatc agatgtttca tcaactgagg
    23401 caaaagatag acagcaaagg tgtgggaagc aacgatccga catacttctt cttcattttg
    23461 ttcattgagc aatttttgct gagtttcttc ccattcttct aacaaaacca gtgtccgttc
    23521 atataataag tcggcttgct tagttggaag catctctttt cttccgttac gttgaaaaag
    23581 atcgatattc aattcccgct ccaactgttt gatttgattc gagactgctg gttgagataa
    23641 aaacagtact tctgctgctc tggaaaaatt tcttgtttca tatactgctc gaaaagtcgt
    23701 taataactta aacatggcac cacttccata actttttatg ataattacta taaaaactat
    23761 ttgatttaaa tatacaaaaa accatctata atagaaatgt acaacaaaac agttgctgat
    23821 ccaactagta acttagatga agacaatcag ctgtctgctt gctcgtaatc atacattcct
    23881 attcatttta caatgtattc gttcatcttg gtactaaaat ctattaaata aaaaggagaa
    23941 tattatatat gaagaacttt ttgaaaatta ttccgatccc gatctgcggg ttgatccttg
    24001 ggttagcttc ccttggaaat ctgtttaaag actatcacca tgttgcttta ggcaatctaa
    24061 ctggcggtat ctctatgatt cttatgatat tcatttttgt taaattgttt atcttattcg
    24121 aacacacgaa acaaacgttg catgatccaa tcatcgcctc cgtttcacca acttttacga
    24181 tgtcactcat ggttatttgt acttattttg tttcatatga aagtattgca ccaattgtca
    24241 aattcatttg gctgtgcgct gttatcttcc aagtgatcct tgtcttttat ttcaattatc
    24301 accatgttat caaagctgat ctttcaattg aagcaattta tccaagctgg tttatcattt
    24361 acgttggatt tggggtcatc actgtaactg ccgggaactt cttcccactt attggaaaaa
    24421 ttttcttttg gatttcattg atctgctatg cgttcttatt gccgatcatc atcaaacgtg
    24481 tctttttcgt aagaaatatg gctcatccga cacgtccgct gattacgatc gttgcagcac
    24541 ctggttctct ttgcctaaca ggttatctta aaaactttgc tgaacctaat ttttatcttg
    24601 tcgtagcatt gttcgcttta tctcaagtat tgtatttcgt tgtgctgtct atgcttccac
    24661 gtttgttcaa gcttcgtttt tatccaagct atgcagcctt tacattcccg cttgttatct
    24721 ctgcaacagc tatatttact actgttcatt acttcaacag tttaggtatc tatagtagcg
    24781 tattggattt cttaaaagtt gcggaatgtc tgtttgcaag tgtagttgtc ttctacgttc
    24841 ttgctcatta cgtacgtttc ttaattaaag aacatagcaa acatggaaaa tttgtttcta
    24901 aaaaaatcgc ttaataatcg attcaatagg ttgtgaaaaa gctgttctgc atcaagtaat
    24961 aagaacggcc aaacaaaaac agctgttcat gtttttgtac tggttgggct tattaccgca
    25021 gatgcaagct tttgaacacc gtttaccaag aggtcgtaaa agaagtggtc agcttcaaga
    25081 aataagaagg aatttacgaa aattgcttct caaatttttg tgaaatttcg gcttatttcc
    25141 gtagaagcta cttctggaac accgtttatt cgaaacaaga ggtcgtacaa gacttttgtc
    25201 acgacctctt gttcttttgt caaaatactg gtttagtgct actatgttcg agtatgcttt
    25261 tagtatttat tgacgaaagg aaccgatcaa atgaaatcat tgtcacatcc aataaaatct
    25321 gtaaaggatt cagcggtccg cgaaatctta cccggtttac tgctttcttt tgccatcgct
    25381 gccttcagca ggcttcttgc tatttatctg ccacagttag gtggagcgac tattgctatt
    25441 ttattgggta ttttattagg aaatactttt tttaaacagc ctgttctagc aatcggcaca
    25501 aaatttgctg aaagcaaact attagaattc tctgtttttt tactaggtgc aactgtcaca
    25561 gctcaaacga ttgctcaaat tggtgtaaaa ggaattgttt ttgtgttact gcaaatgtca
    25621 gtcactatca tcggtgctta cttgattggt aaaaaactgc aattcagcga tacttcttct
    25681 ttgatgatgg ctggaggaaa cgctgtttgt ggttcttctg ctattgcttc aatcgcccct
    25741 tccatccatg cgaaagagga agaaaaagga cagatcatca cactagtcaa tctactgggt
    25801 actgtgatga tgttgactct cccttttctt ggattacagt tgtttggtgg aaatctcatt
    25861 gaaaaaagtg cacttttggg tggtacactc caatctgttg gtcaagtagt cgctggggca
    25921 agcttacttg acaattctgt tgtcgaattc gccatgctat ttaagattct acgtattatg
    25981 atgctcgttg ttgttgtcta tctgtttgaa aaattcatgc acaagaaaca accagctctg
    26041 tttgaaacgc caactgatac gcagaaaaaa cgaaaattac cgcttccttg gtatgtatta
    26101 gggtttgtga tcttttgttt ggtcaacagc ttgttttctt tacctgtagt tattgaccaa
    26161 tcggcacatt ttatcagcag ctggtttgag atcactgctt tagcggcaat tggtctgcgt
    26221 ttagactttg caaaattcat gaaagaaggt acacgcttct tatcttatgg attagctgtc
    26281 gggcttctcc aaacagtgat ggctttacta tttatttggc tgatcaaagt ttagaatgaa
    26341 catgaaaaaa cagttgtgat tctcgcatgg aacaatgtcc gaatcacaac tgtttttaat
    26401 tttgcagata tccttctaac aacaacaatt ggcgtttgat ttcaacccca tctattgcgt
    26461 ttcccatata tcctgtcagt tttccatttt gcccgatcac tcgatgacaa ggaataacga
    26521 ttggcaatgg attggttcga tttgcctgcc caatcgctcg cacagctttc ggactatcaa
    26581 cagcaacggc tacttcttta tacgttcgga tttctccata aggaatggtc gacaaagctt
    26641 tccagacatt ttgctgaaaa ggcgtacctc tctttataga taaaggaaca tcaaattctt
    26701 ttctttttcc cgcaaaatat tcttcaaact gttcttgtgt gcgcgttaaa attcgctgat
    26761 tcgctgattc gctttctgaa attggctgaa aagatacagc agtcaatccg gattcatctg
    26821 catctaacca taaaaagccg ataggtgtgt gtattttcat tttgtttgct cctagtcaat
    26881 agactggaac ttcctccttt ttctctatcc aaaactactt gccaactaat aaaaaatgtt
    26941 cttaaaagtt tgataaaccg aggaaacatg gtaaactaaa tacgttagtt tacaaaaaga
    27001 atacctgttc ttttacgtaa aatcaaattt taaaggtgtt atttaaggtt tattaaattt
    27061 tgaatgtaaa ccatatataa acatacgatt gaaaggatag aaacgatgaa agtatcaatt
    27121 gttgtcccat gttttaatga agaaaaaact gttcctcttt tttttgatga ggttgaaaaa
    27181 ttcaaaggtg cccatgagtt cgaatatttg tttattgacg acggatcaaa agacggtacg
    27241 ttagcagcaa tcaagaagct agcctcagct tatccttatg tccgctttgt ctcattttcc
    27301 agaaattttg ggaaagaagc ggctctgtac gctggattgc aagcttctac aggagattta
    27361 gtgaccgtga tggatgttga tctgcaagat ccgcccgaac tgcttcctga gatgatcaac
    27421 cggatcacta cttctgatat cgattgtgtc ggcacccggc gttcgactcg tgaaggcgag
    27481 cctgcgatac gcagtttctt tgcgaaaaaa ttctaccagt tgatcaataa aatcagcgat
    27541 acagaaatgg tcgatggcgc tcgtgatttc cgcttgatga ctcgtcaaat ggtggatgcc
    27601 gtcctagaat taacagaata caaccgattt tcaaaaggat tatttagctg ggtcggattt
    27661 aaaacagaat atatttctta tgaaaaccgc gaacgtgtgg ctggcgaaac atcttggtcc
    27721 ttctggaaat tattcaacta ctcgattgat ggtatcgtga acttctcgga tgcaccgtta
    27781 acgattgctt catttatcgg cgctttgtct tgtgtcggtt ctggtattgc aattttgttc
    27841 attattttgc gggcattgtt gtttggtgat cctacatcag gatggccttc catggtctcg
    27901 atcttcctgt tcattggtgg aatccaattg ttatgtcttg gaatcatcgg gaaatatatt
    27961 gggaaaatct tcttagaaac gaaacagcgt cctgtttata tcataagaga aacagaaaaa
    28021 gtgaagccaa atgaattgaa gcctcaccaa taatatacgt caaatgaagt aaacattttt
    28081 ttaaatgaaa aaggactgtg acaaaagtct tgtcacagtc cttttttccg aataaatggt
    28141 gttccagaag tagcttcttc ggaaataagc cgaaatttca caaaaatttg agaagcaatt
    28201 ttcgtaaatt ccttcttatt tcttgaagct gaccacttct atcacaacct ctttcatatc
    28261 ttattacttc ttcattgctg cttttagtgc tgctgctaat gggctttctt ctggttcttc
    28321 tgcttgtgca tattttttca gcaagcgtcg ttcttcatgt ttggtcattt tcttcttctg
    28381 ctctttttta tcaagcaatt tttctgtcgt tccatcgtat ttacaacgga aataagcacc
    28441 gtttttacct tcgatgattt ccattttccg gtgacattgc gggcatcgat gattagagac
    28501 ttttggatct tttcttctgc ggtagttgca atctgaattg gtgcagacat agattttgcc
    28561 gtcacgtgtg tttttctcgc gcagattggc cccacattct gggcatttct tttgggtaat
    28621 cgagaaatct tgatattctt gtttgcttga tttgatctca ttcaccaagc gttttgtttc
    28681 tttttcgatt ccattcaaga aggttcctgc ttgtttcttt cctgcagcaa tagcttcaag
    28741 gtctttttcc cagctttctg tcaaatctgg tgttactaaa gagggattta ccaatactaa
    28801 taattgctta cctttaggag atacttgcag tccttgaggg gtacgttcca tcagttcaga
    28861 tttgattagt ttctcgataa tctctgcacg cgtagctggg gttcccagac tgtatttttc
    28921 catttgacca agcaagcttg cttctgacaa tggtttaggc ggtgcagata gttctttttt
    28981 gatggcgaaa tcgggattga ttctcattcc tttttcccat ttgactgttt ccgtctcagt
    29041 ttcagtttcg cctttccatc ctgcttggac gacacgattt tgccgaaaaa tgaatcgttc
    29101 tttaccaaat tcaaccgtaa cttttgtttg gctgacttta tggggttgag caaacagtcc
    29161 taaaaaacga gagacaatca tttgatagat ttttcgctct tcattactta atttttcata
    29221 acgtgctctg ttttcagttg gcaaaagtgc atggtgatcg gtcactttac tgtcttggaa
    29281 tacttttgtc tgttggacga tggctccgtt tttcaaatac tgcttgacct caggagcaaa
    29341 gtcgctgact gcttgcaatc gttctttcat cgtacttttc atgtcatttg tcagatattt
    29401 gctgtctgta cgcggataag taacgatctt atgggtctca tacaaacttt gtacaagact
    29461 taatgttttc tttgcagaat agccatagcg ttgattggct tctctttgga tctctgttaa
    29521 atcataaggc aatggcgcag attccgtttt taatttttct tggacttctg tgacagttcc
    29581 tttgcttgta ctgatcaatt gaacaagttt ttgtgcttct tcttgtgttt ttaaagcaaa
    29641 ttgatttttt tgaagcattt tggcttgttc ttttccagct tgcaacgtga tcgtgaaata
    29701 cgtttgtgga cggaactgct cgattttctt ttcttggtct ctcactaatg ctagtgtcgg
    29761 tgtctgcaca cggcctgctg ataaattatc cgcgtacttc acagttaagg ctctggtcac
    29821 attcaatcct accagccagt ccgcttttgc ccgggcaaga gcagattcat acaatgcatc
    29881 atattctttc gcaggtctta gttgcttgaa tccagttttg attgctttat ctgtttgaga
    29941 agagatccat aaccgcttca ctggtttatt gaaatgcaca tactctaaga tccagcgagc
    30001 aacaagttcc ccttctcttc cggcatctgt cgcaatgact gcttctgaga catcttttcg
    30061 ctgagccagc tgcttgattg cttttaattg atgtcctgtt ttaggaagtg gtttgattcc
    30121 tagtttttta gggatcattg gcaacgtgtc catttgccag ctctgccatt ccttattcag
    30181 atcttccggc attttcaatc ctaataaatg accaagggcc caagtaacga tcaccttggg
    30241 tccttcataa taatttttgt gcttttgatt agcaccaagc accttgctta ggtctttcgc
    30301 aacgcttggt ttttccgcta gaattagttg tttcataaaa ttcctttctg ctttctactg
    30361 tgatgggctg atgatttttc aacaacgcaa gtcccttgta ttgctgtaaa tcatcgtcac
    30421 actcttcaca ccagcgctca tctccgtcat tccaaaaggc gctgagaaag ttactttttg
    30481 cattgatata ttcataattt tgatggattt cgttccattg tttttcgtac gcttgtcgtg
    30541 cttcttcgaa agactcgaat gtttcttctt cttcaatatc ttcttcccag tcttcaaaaa
    30601 accaccaagg ctcattgtcc ccgaacatgg taattacttg atacatactc gtgctccttc
    30661 tttcagcttt tctagactca tcttagtcac ttttcaagaa agttgcaaga attttctctt
    30721 ataaaagggt catttaatga acatcttaaa attgttacaa atgaatctga ttgtttaagg
    30781 caaagtagcg ataaagttat tgatcatcca aaaatagctg tcttctggat cgattgttgg
    30841 ttcttttgta aagtatccta aaaaacgtaa ggaaagataa ccgtggatca tactacgaag
    30901 ttccctgctt cgatgaactt tttcttcttt cgttaattga aaagcctcaa atatctgcaa
    30961 aatgatctga ttcgtttcat ggattccatc taataaaaca gcttcattgg tatgcggtac
    31021 actgatcagt aactcataca ctgcttgatt tgaaaaagca aaggatcgat aagtttctgc
    31081 atagatctgc agtgcttcct gaccactttt tcccacaaga gcccgtcgca gctggtcatt
    31141 aagttcctgc agaagtttcg ctgttagcgc tgttttgaca tcacgaatat ttttgaaatg
    31201 gttatacagc gagggagcct tgatatccaa gcgtttggct aaatgctgaa aggttatttg
    31261 ctgaacatcc atctcttctg ctagttctcg aaagcagtcg atgattttct cttgagacag
    31321 atttcttttt ttcatttcca tgtttcctct cataaaataa atccttcact ctttcagttt
    31381 accacgaaaa agaaagagaa aaaagagact ttcattgata aactacatat tgtagtttat
    31441 aataaagagc tgtagatgaa aattttcaga aagaaggagt ctaattggct gcaagaataa
    31501 aggaaaaatt atttaggaaa agagaaactt gggaacaaaa cttgtttgtc ttatggtttg
    31561 gaacatttat ggctggtatc ggatttagtt tagtcatgcc ttttatgtca ctttacatag
    31621 atacgttagg taattatagt acgtcacaat tgaacttttg gagcggattg actttttcat
    31681 ctactttctt ggtgactacg cttatctctc cttggtgggg aaaattagcc gaccaaaaag
    31741 gaagaaaatt gatgctgttg cgtgcttcat taggaatggc aatcgtcatt tctttgatgg
    31801 gttgcgtgac tagtgtgtat caattagtcg cattgcgtct gcttcaaggg attttttctg
    31861 gttatatcag taatgccact gcccttgtcg caactgggac gccaaaagaa aaaagtggtc
    31921 aagttcttgg aaccttagca actggttctg ttacaggaac attacttgga ccgcttcttg
    31981 gcgggatctc tgcttcagcg tttggctatc gccctacctt tttcattaca gggatcatct
    32041 tatttttcgt cttcttgttg agtctgtttt tcgtccatga aaaatttgtc ccagtgaaaa
    32101 aagaaaacat ggtttctgcc aaacagattt tcaaagactt gaaatatcca cacgtcgtca
    32161 tcggtatgtt cgtcacaacg atgatcatcc aagcatccaa caattccatt agtccaatca
    32221 tcagtctgta cattcgccaa ttgctgcatg gacatgggaa tgttacgttg atcagcggaa
    32281 tcattgcttc gatacctggg attgccactt tgatagccgc tccaagattt ggtcgcttag
    32341 gtgacaagat tgggagtgaa agaatacttg cgattggttt gggttttgcg attctggttt
    32401 atatcccaat ggcattcgtc acgaatgttt ggcaattagc tgccttacgt tttttgattg
    32461 gtatctctga tgcttgtctg ctccctgctg tccaagcatt gatcacaaaa tattcgcctc
    32521 atcaagctgc tggacggatc ttcagttata accaatcttt ccaagcaact ggtaacgtag
    32581 tagggcctat gatcggctcc agtgtttcca gtgcatttgg ttatcgtggc gtctttcttt
    32641 cgacttctct tcttgttttg gtcaacttct tgcttgtacg acacaataca caagagatcc
    32701 atcagcaaga aactgcagaa gaaaaagctt caggagtctc catagcttca gggcactctc
    32761 cttctcatag acaataagcg taaatgaaaa acactgcttg aacagttgtc acagactgtc
    32821 cttgcagtgt ttttgtttag gcctgttttt tcaagaactc agataagagg aataatccct
    32881 tttccaaggt ttctttttta caacagtaac ctagtcgggc atgcttcggt aaatcaaagg
    32941 cacttcctgg tactaatagt actcctgttt cttctaataa attgatacaa aatgtctggt
    33001 catcttcttc gatggctaat ttaatgaaag aagtagagac gtaattggga gcgacaagtt
    33061 caactttcgg ttcattggct acccattgtt ttaaaatcac aaggttctct gtaatgatct
    33121 ttttgtttcg cgccaatatt ttttctttat tcttcaatgc atgtacagca agatcatctg
    33181 acaggactcc gccgcaaatc atcgtgtagt ctcgatactt tcgaaaaact tctgccaact
    33241 caggtccagt agctgtccag ccaatacgaa tacctggaat ggagtaggtt ttggataggg
    33301 aattggttgc tatacctttt tcatagacat ctacgatcga caagaattca cctttatccg
    33361 ttaaaggcgc atatacttca tctatcaata catatgcatc tactgtacga gcaatttctg
    33421 cgattttttc taaagttttc cgatctagca atgtgcctgt aggattgttt gcactattca
    33481 aacagatgat ttttgtttca ggtttgacta aggcttctag ctgttctaaa tcaaactgcc
    33541 aatcttcttc ttccttcaaa tgaacaaagt caacagcggc acctaaagat ttggggatgt
    33601 catataattg ctgataagta ggaagcatcg aaacaacatg gtctcctgct tccactaacg
    33661 cgtatagagc taaaagatta gcaccagtcg ctccattcgt ttgtaagatg ttttccggat
    33721 cgacggtttg ataaagagaa gcgacttctt gtttgaaaac atctgatcct tcgatccagc
    33781 catagtccaa tggttcgttt ttgcgttttt cgaaaaattt acttacgcta gtcccatcta
    33841 agccaacgag ttcgtctaat gtcaatgctt cgatcgagct ttgtgcaata tcatataccg
    33901 ctttcttttc ccatttattt agccattctt ctacaccgaa atttgcgatt ttcatgtttc
    33961 tttccccctt atatcttgat ccagcctaca gcaagcaacc cgattgaaag cagtgcggcc
    34021 acacaaatca gcccaatcat tactttttct tttcccgtaa tttgatgttt ggtttcacga
    34081 caagcgatcc agtagaagca aaaaccagga agataggcaa tactacataa aaggacataa
    34141 gaaaatcctg ctaaaatgat gcccatcaat tcaaaaacta cagcaatccc accgatcact
    34201 acttgttttt tgattccttc ttttaaagaa aactggactt gatagatccc gacaagtaga
    34261 taacaaatca aaattgccgc tgtacataaa gaataggcaa attgataggc ataatcagta
    34321 actaagaaag tcaataagaa tagattagtc aagattgcag taacaactaa cgaataagtt
    34381 ggggcatttt ttttattcgt ttttccccaa cctttcggca acagaccgtc tcgagccatc
    34441 aacatcgttg tttctgctgg gagcatcgtc caagacaacc aagaaccaat gatcgaaata
    34501 atcaagccga tattgatcag cgctgctccc caatttccca cgattccttt taaaatattt
    34561 gccatagcag gttgtgaaat agttgctaac tcttcttgag ataagacgcc gtatggcaag
    34621 atcgaagcaa ggatatagat aaacaataaa caaagcagac caatgatcga tgcttgctgg
    34681 gcttctgaac gtttcttcgc gcggttggct aaaacacttg cgccttcgat ccctacaaag
    34741 acccacatca taaccatcaa acaaccttta atctgcaacc agatcgttcc ttgctgccct
    34801 gttccattca ctagattatc agatacgtta ccccaaaaat cagcagtgaa tatattcact
    34861 ttaaatgcga taaatacaaa tattaaaaat aggaataatg gcactaattt acagatcgtc
    34921 acgattgtat tgatgacgct cgctccttca actccattgt ttactaataa agtcagaccc
    34981 caaataaaaa tacttgcaaa taaaatagaa ggaacatttt gtccaccttt gaatatcggg
    35041 aaaaattctc ccatggcgct catcagcatc gttgcaaaag caacatttcc cagccacgca
    35101 gacaaccagt aaccccaacc actgatgaag ccacctaatt taccaaatga agcttccgcg
    35161 taaccgaaaa taccaccatc tagatctgga cgcttttctc ctaaatgatt cagcgataat
    35221 accaaagcta aaataccaat cccaacgatt atccatgaaa ggatcgctgg ccctggagct
    35281 gcagaactag ccagatcact tgtgatccca aacacaccgc ttccgattga cgaactgaca
    35341 accagagcaa ccagtcccca tttgctaata cccgtcgatt tcttttccat ttccatcacc
    35401 ctcacttata aaattgaaca aatcacttgc aaaagattcc cttctatttt gcaactgctc
    35461 ttgcttaaca aaaagcaact ttattcatat tattggatat tatgaacttt ttacacaagt
    35521 atttcaagta taaaatgaat aaaaattaat tttattgcaa aaaaacagct atgctatcca
    35581 aaagaaaagg tgtttctttt gttagccata gctgttttga ttcatttaat atcgcttaaa
    35641 cgtattgtaa aacttttgtt tcttgatatg cgtggtcgat caaaccacca cccagacatt
    35701 ccattccatc ataaaataca actgcttgtc caggagtgat tgcacgtaca ggttcgtcga
    35761 atacgacttc tgcacgatta tcatccaata agcgaactgt cacaggaacg tcttgctgac
    35821 gataacggaa tttcgctgta cacttgaatt cttttggcat tggttcattc gtcgtaaagt
    35881 ggatttcact tgcatccaag cttgtggcat acaatgcagg atgatggaac ccttgcccca
    35941 catataatgt gtttgtcgct agatcttttc ctacaacaaa ccagggttct tgcgaatctc
    36001 ctccgccacc gatccctagt ccttgacgtt gaccaatcgt ataatacatc aagccagcat
    36061 gttgtccttt gacttcacca tcaagagtga ccatatttcc tttttttgct ggcagatagt
    36121 tgctcaaaaa ttgtttgaag ttcttttcac caataaagca gattcccgtt gaatcttttt
    36181 tcttagcagt cgcaaggcct gcgcgttcag caattgcccg tacttctgat ttttccatgc
    36241 cgcctagagg gaacatcgtt tttgaaagct gttcttgtga taattggcta aggaagtaag
    36301 tttgatcttt attattgtct actccacgaa gcatgtggac cacgccgttt tcatcgcgag
    36361 tgacttgcgc ataatgtcca gtagctacat aatcggcgcc aagatccatt gcataatcca
    36421 agaatgcttt gaatttgatt tctttgttgc acatcacgtc tgggttaggt gtacgtcctg
    36481 cgcggtattc ggctaggaaa tattcaaata cacgatccca atattctttt tcaaaattca
    36541 cagagtaata aggaatcccg atttgagcag ctactttcgc tacatcctta taatcttccg
    36601 tggccgtgca tacaccgttt tcgtccgtgt catcccaatt tttcatgaat attccgatca
    36661 cgtcgtatcc ttgttccttt aacaaaagtg ctgttacaga tgagtcgaca ccgccgctca
    36721 tccctacgac aacgcgtgtt ttgctgttgt ctgtcatgat atcaccatca tttcaattag
    36781 attctgaaac atctacgagt tgaaggtcgc aacttttcag tattttacat tatacgtcat
    36841 atttctgaaa atttcatccc ttaattatca agtcgtaaaa atactgttct actggaccta
    36901 ttcagtacac aaacatgata gtcaaccaaa aaatcactca tcttttgagc attcgatata
    36961 aaaaattggc atactcataa aaagggccag cctttcggca ataaggaata attttataaa
    37021 ttgtgaaaaa ctatttttca aaattgcttc ttatcgctta aagctgcacg tctcatttac
    37081 aaccttttct ttttagatgt tatgagttgc tcagataagt gattttgttt actctaagaa
    37141 aaaagggagg caaaagatac atgaattact ttatcagtga tatgcacttt ttccatgaac
    37201 gcttattagg aaaaaacgac tttgctccgc ggctctttgc aaatgtggat gaaatgaacc
    37261 aacagttgat cactagctgg aatgcagtaa taaaagaaac cgaccatgtt tatcatctag
    37321 gcgaccttgc tatgcatccg caatatgaaa aagcaaatgc agaaattttg gaactggtca
    37381 ctcaactgca aggaacaatc cacttgatca aagggaacca tgacagtcgc gcttttttcc
    37441 gttatttaga aaaaaatgat cctaggctta ctgacggaag tcccaaattt tatttttatg
    37501 atgtaggtgc catcgttaaa ttcgaccatc aacaatatta cttgactcat tatccgatgt
    37561 tgctcgggcc taacgacact atccgcaatc ttcatggaca tatccatcat tacagcgtac
    37621 ctatcgagaa cgatgtcaat gtaggcgtag acgcaccgga aagagaactc ttaaaggaaa
    37681 aactaccatt tggcactccg ttatctgagc aacaaatcaa agaaatctat gaagcaaaga
    37741 ggaagtacga taggtcgtga aaaaactgcc ctatatcaag tattaaacaa ataaagataa
    37801 ttactcatat ttttcatttg agtaggctta ttgccacagg aacattagga tcagaaatgg
    37861 agttgtaaca agacttctgt cacaactcca ttccatacta ttcgtctttt tgctcatggt
    37921 gttcttttac cagttttttc tgtaattctt tatcataatt tcgggcagct gacgttaaaa
    37981 ataaaacgat tccgcataat aaaatcccga ttccggaaaa caagaacatt ttttctaccc
    38041 ctaaacgatc agctaatggt cctgcaaata ataatccaat tggacctgtc aggttcatca
    38101 aagagttgaa gttccctaag acacgaccta aaacattcga ttcataactc tgctggatca
    38161 ttgccatcag cagtgtattg aaatatggcg ttgcacaacc tgctcctgca ttcaagataa
    38221 gaaagtacaa gaacccttga ctatcgtttg gaaccaatcc gcttgcaccg atcgtcaggc
    38281 ccataatgat gtaggctata ataataggtt tcattcggtc tttccatgtt cctataaaac
    38341 tgatcagtgc accgcctaag agcattccga ctgcgtagac tacttccact gctcccgcct
    38401 gtccaacgga accaccaaag taatctagcg tcatcaatgg atacagactg attgctggca
    38461 tgaacaacag catcacaaat gcaccattga tcgttatgta ccataaccct ttattatcca
    38521 tcaattgctg cagcccaaat tttgcatctt taaaaatagt aagcggaact tccaatattt
    38581 caggagaaac ttttgggatc ttcacaaaaa tcaataagcc gacaccaaaa actgccccca
    38641 atacatctaa taaaataagg taattgacag gaacgactga aaacaaagcc gcacctagag
    38701 ctggcgcaat gatgaaattc gctgattgga ccattcctaa ctgtccattc gtccttgtta
    38761 tgtgagaaga tggaacgatt gtcggcataa tcgactggat cgttggcatt tggaatgttt
    38821 gtgcgaccga acgcataaac aaagacacaa aaactagcca aatcgggaag gattctgaaa
    38881 tcgttccgac tactgctaaa ataagcgcga aaatcgctac aataatatcc gtgacaatca
    38941 aaagagcttt tttatcccag cgatccacta gcggtcctac aaatgggctt agtaaaacca
    39001 tgggaatcat tcctagtaaa gtagcaaaac tcaaaattgt cgccgaaccg gtttctcttg
    39061 tcaaatacca gatgatcgca tattggacga ccatactagt gattcctgac aaaaactgtc
    39121 ccgataaaaa gaacaaataa tttctcttcc aattcgttaa aacgaccgac tgttcatttt
    39181 ccataaacat ccctcttttc tttttcattt tgtatagcgt ttgcacaaca atcattatac
    39241 gggattcttt tttcaaaaaa tacctttttc cctaaataac aatcgtttca atttaaattt
    39301 ctttacttag aaacaataaa tcagcaaaat attaaaaata acaacatgtg ttaaatttaa
    39361 aaataaaaaa gacaactaat ttgcgtcgtc cttacaaagt tagtcatcta acaataggcc
    39421 gtttcaaaac tagttgtctt tttatatcca cagatcattc gactttttcg aataacccac
    39481 ggctaatcat cccatccatc agaaaataaa atttatcttg gctcattttc tgttttggat
    39541 ctttgaaaat attgactgct ttcaatccat ttgctgtagt gatcgacatt ttcaatgttt
    39601 gaatatcttt tgatacagtg atttttaatt tgaatccttc tttgctttgc ggcgtggcat
    39661 ctaatggtcg gatcaattga taattgcctc cgtttgtttc tgtaaatcca ttatcgcgca
    39721 atgtatatct ttttgcatca ggatgcaaac gatagctaat tggtgcgccc agtaccgttg
    39781 ctgttgtttc aaatgccatg attttcacct cgataaaatt agtttctctt tattataaaa
    39841 gtttatcctg atttttacta ggtttttcag ttaagttccc taaaaaatga aggcgttctc
    39901 tcagacatct gttcatataa gaatgacttc actagttatt ttttgacagc acgaactaat
    39961 tgatcaagga aatactggat gtcgtcttcg gtatttccca aaccaaagct gatacggatg
    40021 gattctttta gtatcggtgt atcttctcca tacattgctt ccaatacgtg tgaaggctcg
    40081 atatttcctg ctgtacaagc tgaaccagta gaaatagcga ccccattaag atcaagcttc
    40141 atcagtagca aatcatttgc ttcgccaatt aagcgaagat tcaacacatg agggagttta
    40201 ttcgttaaat caccatttac ttgataaggg atttcgtttg cttctaatgt atcaagaatc
    40261 aattgctgga atcctttata tttgttgtta cgtgcttcac gttcttcagg tgacagcagt
    40321 tcaactgctt ttgccatacc ccagatccct gctaaatttt ctgtacctgc acgccgcttt
    40381 tcttcctgct ccccgcctaa aagcaatgca gggatattta ctccgtctcg tttatataag
    40441 aaaccgacgc cttttggccc attgatttta tgagcagaga tacttaataa atcgattcct
    40501 aattcttctg gatggatcgt ttggtttccg tacgcttgta ccgcatctgt atggaaataa
    40561 gctggatgat ccgctaaaag ttcaccgatt tctttgattg gcatcaaatt tccgatttca
    40621 ttgttcccgt acatcacaga gaccaagatc gtatcttctc gtaacgcttt tttgacctct
    40681 tcgatcgaca gattcccttt ttcatctaca ggcagatatg taacgtcaaa cccctttgtc
    40741 tctaaatatt tcatggtatt aagaacagca ggatgttcga tagctgtagt gattagatgt
    40801 ctgccttctt tttgacgcga gaacgctgtt tctaagatag ctgtattatc cccttctgtt
    40861 cccccactgt taaagatgat ctcgtgaggt tttgcttgca gactgtctgc aatgatttgt
    40921 cttgctgttt ccaatttttc atgcgcgtgg cgaccaaatc catgtatgct tgaaggattt
    40981 ccaaacgtgt cttgcatgat tgccgtcatc gcttcaatta cttgtggatg catgggggta
    41041 gttgccgcat gatccagata aatagttttc acttcattca tcccttccaa attattgcta
    41101 ggcacattct agcataaaat agcgaacaaa ccaaagaaga gctcattttt gtctagagga
    41161 cgtttcatgg gcatagaaaa aaggagctgt gacaaaagtc ttgtcgcagc tccttgaagc
    41221 tgctcacttc tgtcacaact tctt
//
LOCUS       DACDXO010000019        39223 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E23605_2017
            SAMN12639989-rid9881463.denovo.019, whole genome shotgun sequence.
ACCESSION   DACDXO010000019 DACDXO010000000
VERSION     DACDXO010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639989
            Sequence Read Archive: SRR10023389
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 39223)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39223)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741914.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:42:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,972
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 33 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 17 of 73
            Pseudo Genes (multiple problems)  :: 10 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39223
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639989-rid9881463.denovo.019"
                     /isolate="E23605_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            131..505
                     /locus_tag="IWM35_001177"
     CDS             131..505
                     /locus_tag="IWM35_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAP9919043.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(619..1248)
                     /locus_tag="IWM35_001178"
     CDS             complement(619..1248)
                     /locus_tag="IWM35_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAP9919044.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1382..1454)
                     /locus_tag="IWM35_001179"
     tRNA            complement(1382..1454)
                     /locus_tag="IWM35_001179"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1419..1421),aa:Thr,seq:ggt)
     gene            1668..1741
                     /locus_tag="IWM35_001180"
     tRNA            1668..1741
                     /locus_tag="IWM35_001180"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1702..1704,aa:Asn,seq:gtt)
     gene            1696..1845
                     /locus_tag="IWM35_001181"
     CDS             1696..1845
                     /locus_tag="IWM35_001181"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9919045.1"
                     /translation="MTVNHDVVGSSPTAGVNKAYAKKKKSNHLGFFFFYYYDESSFLF
                     LSRML"
     gene            2338..3666
                     /locus_tag="IWM35_001182"
     CDS             2338..3666
                     /locus_tag="IWM35_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HAP9919046.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            3868..4701
                     /locus_tag="IWM35_001183"
     CDS             3868..4701
                     /locus_tag="IWM35_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HAP9919047.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            4717..5454
                     /locus_tag="IWM35_001184"
     CDS             4717..5454
                     /locus_tag="IWM35_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HAP9919048.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
     gene            complement(5609..6577)
                     /locus_tag="IWM35_001185"
     CDS             complement(5609..6577)
                     /locus_tag="IWM35_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HAP9919049.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELEKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            6809..7642
                     /locus_tag="IWM35_001186"
     CDS             6809..7642
                     /locus_tag="IWM35_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HAP9919050.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            7676..8515
                     /locus_tag="IWM35_001187"
     CDS             7676..8515
                     /locus_tag="IWM35_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP9919051.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            8541..9101
                     /locus_tag="IWM35_001188"
     CDS             8541..9101
                     /locus_tag="IWM35_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAP9919052.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            9300..11021
                     /locus_tag="IWM35_001189"
     CDS             9300..11021
                     /locus_tag="IWM35_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HAP9919053.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            11186..12328
                     /locus_tag="IWM35_001190"
     CDS             11186..12328
                     /locus_tag="IWM35_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAP9919054.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            12344..13555
                     /gene="thiI"
                     /locus_tag="IWM35_001191"
     CDS             12344..13555
                     /gene="thiI"
                     /locus_tag="IWM35_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HAP9919055.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
     gene            14128..14775
                     /locus_tag="IWM35_001192"
     CDS             14128..14775
                     /locus_tag="IWM35_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HAP9919056.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKMLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     gene            15168..17813
                     /locus_tag="IWM35_001193"
     CDS             15168..17813
                     /locus_tag="IWM35_001193"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HAP9919057.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            18123..19436
                     /locus_tag="IWM35_001194"
     CDS             18123..19436
                     /locus_tag="IWM35_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HAP9919058.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            19426..20079
                     /locus_tag="IWM35_001195"
     CDS             19426..20079
                     /locus_tag="IWM35_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP9919059.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDLEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            20134..20685
                     /gene="radC"
                     /locus_tag="IWM35_001196"
                     /pseudo
     CDS             20134..20685
                     /gene="radC"
                     /locus_tag="IWM35_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736077.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
     gene            20816..21901
                     /locus_tag="IWM35_001197"
     CDS             20816..21901
                     /locus_tag="IWM35_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP9919060.1"
                     /translation="MKVQRIEVENKPYPLYLLLDKEYQLIEPVMKFIKYLDNTGKSPN
                     TIKAYCYHLKLLYEFMEQRGVILNDINFELLADFVGWLRYPSASNVIDLQSKKAIREE
                     TTVNTILNVVMSFLDYLSRLGEFKSIDVFKQAKGRNFKGFLHHVNKGRYQKNVLKLRV
                     KKKQIRTLRSEEVKQIIDACHTKRDKLILMLMYEGGLRIGEVLSLRLEDIVTWDNQIH
                     LTPRDVNVNEAYIKLRKERTIHVSKELMSLYTDYLIYEYSEELEHDYVFISLKEGYFG
                     KPLKYQSVLDLVRRIVKRTGIEFTSHMLRHTHATQLIREGWDVAFVQKRLGHAHVQTT
                     LNTYVHLSDQDMKNEFNKYLERKEHKK"
     gene            21898..23790
                     /locus_tag="IWM35_001198"
     CDS             21898..23790
                     /locus_tag="IWM35_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001833801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP9919061.1"
                     /translation="MNASSKRKIISQSEISKKIAVMNEEMQGFWANNSWDIRKCPHPS
                     AIELSKNPALRNRWVRFERVKNLWLRTELKYFYFYHLNNGIWNAKTVWIRKGTVINKM
                     LDFLDLKYPSITSITEVPIEKAMTEYRTYLTKRGVRITTTNYKITANQEKTPVKANSY
                     YVTNLKQFMEFYENFYFDGEEWDKDVWDRRNLPLPDDKVNPTQYEYTINFKGFRNTYF
                     KQLVKRYCKLRLNMDSFSYVSDIAQRLKEFFNFLDMKFKQVQRVHQLTRVEIEAYLSE
                     LNMMGIKPSTITGRISILEGLFSTLLRLEWDDVPSKILIYSEDYPKIPRAKPRFIDEF
                     VLEQLNSHLDKLPEYIATMTMIVQECGMRISELCTLKKGCLLEDKDGDFFLKYYQWKM
                     KKEHIVPISKEVALLIKVREDKVSEEFPDSEYLFPRKDGSPLKQETFRGELNKLAYEQ
                     NIVDKSGEIYRFHAHAFRHTVGTRMINNGMPQHIVQKFLGHESPEMTSRYAHIFDETL
                     KNEFTKFQEKLVTNNGDVLDLDEDNEVDDVELQWFKKNINAQVLPNGYCRLPVVAGGC
                     PHANACLDCTHFCTSKQFLPQHEEQLERTEELLAIAKDKQWQRQVETNSRVKERLEQI
                     IGSLTG"
     gene            23797..24174
                     /locus_tag="IWM35_001199"
     CDS             23797..24174
                     /locus_tag="IWM35_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP9919062.1"
                     /translation="MDKQVRNTTEIVRLAKQKSKKTREKVDKAISKFSIEGKVINFNS
                     IAKEANVSKSWLYKEHDIRQRIESLRERQITANVVSKPKKSSRSEEILIKTLKRRVME
                     LEKENKKLQNQIQKLYGDLYNKE"
     gene            24328..25110
                     /locus_tag="IWM35_001200"
     CDS             24328..25110
                     /locus_tag="IWM35_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(9)-Ia"
                     /protein_id="HAP9919063.1"
                     /translation="MSNLINGKIPNQAIQTLKIVKDLFGSSIVGVYLFGSAVNGGLRI
                     NSDVDVLVVVNHSLPQLTRKKLTERLMTISGKIGNTDSVRPLEVTVINRSEVVPWQYP
                     PKREFIYGEWLRGEFENGQIQEPSYDPDLAIVLAQARKNSISLFGPDSSSILVSVPLT
                     DIRRAIKDSLPELIEGIKGDERNVILTLARMWQTVTTGEITSKDVAAEWAIPLLPKEH
                     VTLLDIARKGYRGECDDKWEGLYSKVKALVKYMKNSIETSLN"
     gene            complement(25236..25967)
                     /gene="erm(A)"
                     /locus_tag="IWM35_001201"
     CDS             complement(25236..25967)
                     /gene="erm(A)"
                     /locus_tag="IWM35_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001072197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(A)"
                     /protein_id="HAP9919064.1"
                     /translation="MNQKNPKDTQNFITSKKHVKEILNHTNISKQDNVIEIGSGKGHF
                     TKELVKMSRSVTAIEIDGGLCQVTKEAVNPSENIKVIQTDILKFSFPKHINYKIYGNI
                     PYNISTDIVKRITFESQAKYSYLIVEKGFAKRLQNLQRALGLLLMVEMDIKMLKKVPP
                     LYFHPKPSVDSVLIVLERHQPLISKKDYKKYRSFVYKWVNREYRVLFTKNQFRQALKH
                     ANVTNINKLSKEQFLSIFNSYKLFH"
     gene            complement(26025..26112)
                     /locus_tag="IWM35_001202"
                     /pseudo
     CDS             complement(26025..26112)
                     /locus_tag="IWM35_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771940.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ErmCL family antibiotic resistance leader
                     peptide"
     gene            26476..27138
                     /locus_tag="IWM35_001203"
     CDS             26476..27138
                     /locus_tag="IWM35_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAP9919065.1"
                     /translation="MNTIESQFDKVAEDYDFVNELLNDYSFFVSNMSPKKGRALDIGC
                     GSGLLVEKLASYYDEVVGIDISNQMLDLAKSKRQLTNTVYLNMNAEQLNFNEKFDFIV
                     SRTTFHHLDDIASVIQQMKELLNEEGRIVILDNVSEVETPPTYVYKLGAIQEFLPHCF
                     KFGIKNAIRIYNHNTSKSWLEHLASDKYLSEQNYYDLYEKLLPGCQFHKMGWAMGVVW
                     TK"
     gene            27373..27516
                     /locus_tag="IWM35_001204"
                     /pseudo
     CDS             27373..27516
                     /locus_tag="IWM35_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein"
     gene            27666..27866
                     /locus_tag="IWM35_001205"
     CDS             27666..27866
                     /locus_tag="IWM35_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP9919066.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
     gene            complement(28120..29799)
                     /locus_tag="IWM35_001206"
     CDS             complement(28120..29799)
                     /locus_tag="IWM35_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HAP9919067.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
     gene            complement(29801..30013)
                     /locus_tag="IWM35_001207"
     CDS             complement(29801..30013)
                     /locus_tag="IWM35_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HAP9919068.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            30406..32136
                     /gene="efrA"
                     /locus_tag="IWM35_001208"
     CDS             30406..32136
                     /gene="efrA"
                     /locus_tag="IWM35_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HAP9919069.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            32133..33893
                     /gene="efrB"
                     /locus_tag="IWM35_001209"
     CDS             32133..33893
                     /gene="efrB"
                     /locus_tag="IWM35_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HAP9919070.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            33988..34185
                     /locus_tag="IWM35_001210"
     CDS             33988..34185
                     /locus_tag="IWM35_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9919071.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            34338..34823
                     /locus_tag="IWM35_001211"
     CDS             34338..34823
                     /locus_tag="IWM35_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HAP9919072.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(34927..35520)
                     /gene="clpP"
                     /locus_tag="IWM35_001212"
     CDS             complement(34927..35520)
                     /gene="clpP"
                     /locus_tag="IWM35_001212"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HAP9919073.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            35700..36227
                     /locus_tag="IWM35_001213"
     CDS             35700..36227
                     /locus_tag="IWM35_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HAP9919074.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            36318..37055
                     /locus_tag="IWM35_001214"
     CDS             36318..37055
                     /locus_tag="IWM35_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HAP9919075.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            37059..37976
                     /gene="miaA"
                     /locus_tag="IWM35_001215"
     CDS             37059..37976
                     /gene="miaA"
                     /locus_tag="IWM35_001215"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HAP9919076.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAADFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            37978..39207
                     /gene="hflX"
                     /locus_tag="IWM35_001216"
     CDS             37978..39207
                     /gene="hflX"
                     /locus_tag="IWM35_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HAP9919077.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
BASE COUNT        13178 a         6424 c         8149 g        11472 t
ORIGIN      
        1 ctacactctg agatgtaaga aatatcataa gatatttctt acatcttttt gttttgagca
       61 ggactttaga taattttatt aatatatgct agcatgcttt tgtaagaaaa agaaagaggg
      121 cgtattagtt atgtatctta aaattacgga acaagcacaa gaaagattac aaaaatatat
      181 tgatgaaggc gcaacagtta tattggattt agatgatggc gttggggaat attctaaaat
      241 gggtgtttgc tctttagata ctagttttcg tttacttctt cttgataaag aacagagaaa
      301 aaatgattat aaactccaat tagatagtga tattggggat gtttatataa aagattattc
      361 aaaaatgtac atggatgaac atatgaccct ttctttagat ccacgtctag gtgtatttac
      421 tcttgaaagc ccaagtggtt ctttagacag tcatgttcaa ttagtagatc tacgaccaga
      481 aaaattctct ggaataaaac aataactttc tctctattgt ccataaaact agtctcaatc
      541 aagctgtatt gaaaaaacaa gacaacattc ttaccatggt aaataaaaag acgacacatt
      601 ttgtcgtctt tttttaaatt aactattttc gataaaagat cgcatagctt gtgccattat
      661 ttctggcttt tctctattca attcatgttt tccgttttta ataaagagta gccggccatt
      721 cgttgaaagt ttatataaac gttttgctgc tggtttattc gcatgatctt tggtaccgca
      781 taaaatcaat ttaggaagtc gtgatgtacg aagctgtttt ttgcagtcta atgtttcaag
      841 tgaacgaagt agggtaaggg tttgtttttt agatagacct aatttttcaa atatcttaga
      901 aggtacgcag taaaaataaa tattctggaa agtaagcaat aagcgattag gagactcaaa
      961 ttgtggagcg actaaaacaa ccttttttaa atatggagaa ggagttgcaa gatagtttaa
     1021 agcaataaca gcgcctaaag ataatccaca taaaataaaa ggttccttta tctgattcaa
     1081 ttgtacttcg atgtatttgg ttaagtcgtc taaagtagca gtgtctgtgc tgtttaaatg
     1141 attgaacaat gaaagacttt tgcaagaata actattgggt agataagaga ttacatcttt
     1201 ccatgattgt tcgtcttggc ctagaccatg caaaaaaaca attttcatat tgctctcctt
     1261 tttatagtta tcatatagac tttctttgta ctattaaaat ataaacattc atttcttacg
     1321 tcataaaata aaaaaacagc gcagacacat tgttaaacca acatgttgca ctgtttaagg
     1381 aagccacttg ccgggcttga accggcgacc tcttccttac catggaagcg ctctaccagc
     1441 tgagctaaag cggcattatc attaatacaa ataataggat aacgaatatt aatgtatttg
     1501 tcaacagaat tttttcgaaa aaatgtgttt ctctaatagg aaaacagatt ttagaaaaaa
     1561 taaaaataac aaaaaatgcg aacggtttct ttgataatat aataaaaaaa ttcaaaaaaa
     1621 gtattgcgtt atttagaaga aattgatatt atattcatgt acttgattcc ggcatagctc
     1681 agttggtagt agcgcatgac tgttaatcat gatgtcgtag gttcgagtcc tactgccgga
     1741 gtaaataaag cttatgcaaa gaaaaagaaa tccaatcact tgggtttctt tttcttttat
     1801 tattatgatg aatcaagctt tctgtttttg tcacgtatgt tgtaatcttt acgaaagagt
     1861 agagttggct actcataatc aaccatactt cccatcatcg ctaaatacga aataaatcag
     1921 cattcctaca ccaatcaaag cacaagctgc accaagaaaa gggatgacat tcccaaagtt
     1981 atctgtattc ataagtatgc caccacccac aaacaaaaag ccgccagcaa tcaggttttt
     2041 cattttattc atctcctttc ttacgttata gtatttcctt ctttcaatga gtattttgaa
     2101 agctatctta ttgtatgatg ttcttctagg cctcttcttt attatacagg ccattttcgt
     2161 tatctcatag aaatattcct ttttatctga aaactattgt ataccgtatg acatttttct
     2221 tcatgaagtt gtaagtatat tctcattcaa atgaaaattt catcatagaa gagattgtat
     2281 ttctaaattc ttaagagtaa aatacgaagt ataacttgga tttggaagga tgattggatg
     2341 aaagctatta cgcctgatat gacaacaaaa tttaacaaac agtttcaagc aaatatcaaa
     2401 cagcaggcat tgcagcgggc agttgtgaag aacggaatta ctgcttctgc tgaaaatgtt
     2461 tcagcacatg tggagaatac accggtattt tctatagatt tggctacggg gaaagtggct
     2521 aatcaaatgc aatcggggcg ttgctggatg tttgctgcac tgaacacatt ccgtcataag
     2581 atgctgaatc aattccattt aaaagagttt gaattatcac aaaattacac ttttttctgg
     2641 gataaatatg aaaaggcaaa ttatttctat gaaaacatca ttgcaacagg aaaagaggca
     2701 atcgatagcc ggaaagtagc tttcttactc gctactcctc aacaagatgg cggccaatgg
     2761 gatatgatcg tttcattatt ccaaaaatat ggcgttgtgc ctaaatcggt gatgccggaa
     2821 agtgcgaata gttcaaattc tcgagatttg aataactact tgaacaaatt actgcgtaaa
     2881 gatgcagtag tgttacggaa aatggttgct caaggagaaa cattagacgc tattgaagaa
     2941 aagaaagaag aaatgctaga aagcatttat aacttcttag ctatctcatt aggaacacct
     3001 ccaaaagaat tcgattttga gtaccgcgat gaagaaaaga actatcatct tgaccgagga
     3061 ctgacaccac aaatttttta tgataaatac atcggtgtaa aattagatga ttatgttagc
     3121 gtgatcaatg caccaacaaa agacaaacca ttcaatcgtt cttatacagt agagatgcta
     3181 ggaaatgtcg ttggcgggaa agaagtcaaa tatctgaatg ttgatatgga aactttcaaa
     3241 aaattagcga ttgctcagct tgaagaagga gaatctgttt ggtttggttg tgatgtaggt
     3301 caatcttcta ctagaactag tggcatcatg gccttagatg cttattcaat ggatgatcta
     3361 tttgatacgg attttacaat gactaaagct gaacgtttag attttggaga aagtctgatg
     3421 acccatgcga tggtattgac cggtgttgac ttagtagacg ggcaatcaac aaaatggaaa
     3481 gtcgaaaata gctggggcga aaaagttgga aagaatggct tctttgttat gagtgatgcc
     3541 tggatggacg aatatacgta ccaaatcgtt gtaagaaaag atttattgac gaaagagcaa
     3601 ttaaatgcgt tcaatgaaga accaattgtt ctatctccgt gggatccaat gggtgcttta
     3661 gcatagacga gtatttcaat gaagagtatg tagtaaagat aaaaagagct ggcttaagcc
     3721 agctcttttt atctttaaag tccattccct gttttttatg gtccctgtgc tataattcta
     3781 aataaaggat aaggattgga aataatcctt ttatcattaa aaatatgatg acgaagtaga
     3841 ataaaattgg gagtaggggg gaaagtcatg gatatccaaa ctgttttatc tgatctgaaa
     3901 ttaaatggta aagtgattcc agttgttggt ggcgatgtta atcagaccta tcgtataaaa
     3961 accgaacatc gtgcttattt tttaaaaatc catccgaatg taaaaaaagg attttttgaa
     4021 gcagaagtag acggattaaa agaactgtca gcattcgtac gtgtaccgga tacgtatatg
     4081 ctaggtgaaa caagtgaagg cgcttatttg ttaatggaat ggatcgagcc gggaaaaggg
     4141 gatcaacgag atcttgctgc agctttagct aatctccatc agcaaacagc gccacaattt
     4201 ggttttcgta aagataacta tttgggtaca cttgttcaga aaaattcttt tgaagaagat
     4261 tggtggactt ttttctttaa agatcggctt gaatcccaga tttctttggc agaagaaacg
     4321 aatcgatgga atgtacagcg tcaagaaaag tatctgcgat ttaaagaacg tgtattgaaa
     4381 tcggttgaac caaaaaagat cactccacgc ctcttgcacg gagacctgtg gagtggtaat
     4441 gtgttctttg accagcaagg gcatccagtt tttgtcgacc cagctgtttc ttatgggaat
     4501 cgggaacaag atattgcaat gagccagtta ttcggtggtt tccgaccgga atttttagat
     4561 gcttatcaaa ctatatttcc attagaaaag ggatggaaag accgactgcc gatttaccag
     4621 ttatattatc ttttggcaca tttgaatatg tttggagaaa gttacggttc ccaagtagat
     4681 caattactag aaaattttta agaggagggc gtgtttttgg caaaaaaata taccaaaaag
     4741 catgaagttt catattatga gtgtgacatc aaccaaacga tgacatttcc ttcgatgtta
     4801 ggagtagtta tcaaaacgtc tgaagatcaa agcgaccagt tgggaagagg cagtgaattc
     4861 gtcaatagct ttggacttac ttgggtcatc acgaattatt ccatggagat taatcgtctc
     4921 cctagagtcg gtgaagtaat ctcagtaacg actcaagcca tggagtacaa taagtttttc
     4981 tgttatcgga acttctggat ccatgatgaa gatgggaaag aattagtcaa aatagaatct
     5041 gttttcgtat tgatggattt tgtgaatcga aaaatgagca gtgttaatga agagatcatt
     5101 gctccttttg agagtgagaa gatcaagaaa atcaagcgtc aggaaaaaat tgaaaagatt
     5161 gattcgggct tcatgttgcc atatcgtgtt cgtttttatg atatcgatag taatcagcat
     5221 gttaataatg cgatgtattt taattggatc attgacgtat taggatatga ctttttgact
     5281 acgcatgttc cagaaaaagt aattattcga tttgataaag aagtagagta cgggaatgag
     5341 atcgaaagcc attttgaaca aatccctatc gaaaatggtg tcaaaacaaa acatgaaatc
     5401 cgaatgcaag aacagctgta ttgtgaagca aatattatat ggaaaaataa ataaaaaaga
     5461 ggttgtgaca gaagggggca acttcaagaa ataagaagaa atttacgaaa attgtttctc
     5521 aaatttttgt aaattttcga cttagttttc gaagaagcta tttttgtcac agcctctctt
     5581 tttaagttta gctattagat aatgatgttt agttttggat aaacgaaatc aattcttcac
     5641 ccgacaaacc atagtttaca gcaatcaata gttttaaacg tgctttttga ctattaatac
     5701 tgttacagaa aataatcccc attttttcta gttcttttcc cccgccttca taatcataaa
     5761 caggctctgc gattccattg aaacaacgag aaacaagtac tacagggatt ttttggacaa
     5821 gaagcttttc caaagcagct aaggcttgtg gagggagatt ccccgcacct aaagcttcga
     5881 ttaccaaacc gtctactttc gtatgggcga tggcttccaa taagtcccct tgcatcccag
     5941 catatgcttt aacgatcggg atagtcccat ctacagcgga aatgtccagt cgctttgttt
     6001 ctaaaagttc ctgcaagaat aaaattcgat tcttagttac taagccgatg ggacccaatg
     6061 taggtgttcg gaaagtagca acatttgtgg tatgggtctt tgtcacgtag cgtgcagaat
     6121 ggatctcatc gttcatgacc accaaaaccc ccttatctag cgcttcttca caacttgcga
     6181 cacgaatcgc actttcaaaa ttataaaggc cgtcacttcc aagttcatta cttgaacgca
     6241 ttgccccagt caaaacaatt ggtaatttgt tgccaatcgt tgtatcaaga aaaaaagcag
     6301 tttcttctaa tgtatctgta ccgtgtgtga tcaccactcc ggatatacct gatgcataag
     6361 cggacaagat acgttctttt aataccaaca tgtgagaagg catgatgtgt ggtgagggaa
     6421 gattgaagat atcttcaact aataattcta catgcttggg gattgtgatt tctgcatcta
     6481 ataatgggtt tgccacatca ggactaacac cgcccgtgag catgttttct ttcatggcga
     6541 tcgttccgcc ggtgtgtagt acaagtattt ttttcatttt acagacttcc ttacgaataa
     6601 ttttgcatag ccgttaaatc ttattgtata ctatataaaa gagttttgct atcagtgaag
     6661 aataacgaat agcctaactt atcataaaaa agctgacctt attaaaatgg aaaagactca
     6721 accttgacct cctaatacaa tggagtatct ggttgagttt cttttacaag aaagcagcca
     6781 aggaaatcaa tcacgaaggt ggccggaaat gatcagaatc ggattaacat cgtttaaaga
     6841 acatgacaaa ttaactggta aaaagaataa cactctttat gaatatgcag ggtttctacc
     6901 cttagtggaa ttagatactg cctattatgg aattccccga aagtcctcag ttgagaattg
     6961 gataagtgaa gtacctgaat catttcgctt tgtagtcaaa gtctatcaag gaatcagcgg
     7021 tcaaggagac tggaagcctt attatcagac cgaagaagag atgattgcgc attttttaga
     7081 tgttatgtcg ccaatggtag atgctggaaa gatgttttgc tttttagtcc aattcccctc
     7141 atcattcaaa tgtacgaaag aaaatgtcca gtacttgcgc aaacttagaa agtggtttga
     7201 gggttatcct gtttctatag aattgcggga ttattcttgg tacagcgatg aatttcttga
     7261 acgtacaaga aactttatgt ctagagccgc tttttcttta gccatcatcg atgagccaca
     7321 gttgctgaat acaacgattc cttttgatcc atttgtcacc aatccgaact ttgctctttt
     7381 tcgattccat ggtaggaatg cgcaaggatg gcaagcaaat gataaagagt ggcgtaaaaa
     7441 aagaacgtta tatcgttatt cagaaaaaga actgcaagaa ctagcacatg aagtcaaagt
     7501 agttgattct aagacaaaag aggtaggtgt gatttttaat aataattctg gtggtgatgc
     7561 ggctgaaaat gcattagcac tgcaaaaaat attaaatatc aagccagatg gactcaatcc
     7621 aacccaaatg gatctgtttt aagggcttat tttattcaag aaaggggcga agtctatgat
     7681 caaagctatt ttttttgata ttgacggaac attggtcacc aatcgttcaa aagcgttgga
     7741 atcaacaaaa caagcaattg aatatgctcg gaaaaacggc attttatgcg gtgtagcaac
     7801 ggggcgcagc ccagtaaaga tcaaagaaat cattgatgaa ttggaattag atatgtatgt
     7861 cgtgtataat ggtcaactgg tttttactgc agatcgcacg attatcgacc acccttttga
     7921 acaaaaagta ttggaacata ttgttgaatt tgcagatgag aaccatcgac aaatcgtttt
     7981 tggtgcgaga aatcggttag atggatcaac gacgatgcta ttaggacaat cgatttttat
     8041 caagcgacta gtcagctttt taccgcgtaa attcccggta cgtctgatga aaaaaatcct
     8101 ccaggttttt agccctcacc gacaaaagga tcgttatgaa acgttagaga tattaaaaga
     8161 accaatttat caatgtatat tgctcagtcc ggagtcagaa cagaaaaagt tagaacatcg
     8221 ctttcccgag tgtacgtttc agcgttcaaa tacgtatact gtagatatca tcccaaaagg
     8281 tggttcaaaa ttactaggca tccaagcttt cgcaaatgct gtcgaaattg aaatggaaga
     8341 aattatggca ttcggcgatc attacaatga tatcgagatg ctgaaaggtg taggaattgg
     8401 agtggccatg gggaatgctc agatcgaagt aaaacatgct gcagactttg tgactcaatc
     8461 aaatgaagaa gatgggatat acgaaggact gaaacacttc ggtctgattt attagtcaat
     8521 gaaagaagga aaaaatcagt atggaagcag agacaccttt tcaaaaagta gaaacattcc
     8581 acaaaacttt tgatccaaga agaccagaga tacccacggc ttttacagaa aatgaagctc
     8641 atacacgtgc tgcgttcaaa atagaagaac ttgttgaatt tgttgcagct acttcaccta
     8701 ataaagaaca actgcatgaa gctgttatgc tgctgcacga agcattagat aaagcagaag
     8761 aaaaaatact tggtaaaaca cagtggggaa gtgatccgct ggttgaacaa gtagatgcac
     8821 tgacagatat cctttacttt acttatggtt ctttttcatt actaggaata gtgcctgatc
     8881 gaatattcga aatcgtccat caggcaaata tggggaaatt atttccagat ggaaaacctc
     8941 attatgaccc tgtaacaaat aaagtgttga aacctgaaaa ctggcagatg gaatatgctc
     9001 cagaacctaa aatcgcggca gaaatccaac gtcagattca agaagcgatt gctaagaaga
     9061 aatccgctga tataggcatg tgcacaaatg aaaaagagtg atcagagttt cactattttt
     9121 gttatcttta tacgatggaa aaacaaaaga ttttcgttta agttagtgaa tgcatgaaaa
     9181 tagtatgcta taatctagaa tagatgtctt ttgcacgaga gacgtattat ttggatttat
     9241 gccaagcaga acgccatctg ttgcataaaa gatgcatact gaaaacggag gaaccataaa
     9301 tgggaaataa tgttatcatc ggaatcgtca ttgcaatcat tatagtcgcc attgtcgtct
     9361 ttttggttag ttacttgatg aagaaaaaaa accaagatcg attggatgcc ttagaaaaac
     9421 gcaaggaaga attatttgac ttgccagtga ttgaagaagt cgatgatgtt aaaaaaatgc
     9481 atctggttgg gcaaagccaa aatacctttc gtgaatggag tcaaaaatgg aatgacatat
     9541 ctacttcctc gttcgctgaa ctagaaagtc aaattttcga agtggaaaac ctcaatgaaa
     9601 gttttcggtt cttaaaagga aaaaaagcag ttgttgaagc agaagctaca atggaccaaa
     9661 tggagaaaca agtgacggaa atccgtgaag gtctaaagga attgcgggaa acggaagaac
     9721 gcaactcact agctgttcag caggcacttg acgtctatga agaactgaaa gaaaaagtca
     9781 gagaagacga gcatgaatac ggaccagctc tttttgaatt gcaaaaacag atcaaagtga
     9841 tcgaaaaaga atttacgcag tttgtgactt taaacacttc tggagaccct gttgaagccc
     9901 gcgaggtttt ggataaagca gaaaaacata cgtatgaagt ggaagctctg atgaaaaaaa
     9961 ttccaccgct gtatgaggag ctccatacca ctttccctga acaacttgaa gaaatcagtg
    10021 atacttacga gaaattgatg gaagaacaat atgtattccc agaagaagat cttgctgaag
    10081 atatcgcaaa agtaagtcga agaattgaaa attcattagc caatcttgaa aaaacagaag
    10141 ttgaaacggt cgaatttgaa aaccgtgaaa cagctgattt gattgattcg ttatatgata
    10201 tcttagaacg ggaaatggaa gctcagagat atgtaaaaac taaccaatca acgattgcag
    10261 agtatatcaa acatacaaca aagaataacc gtcaattatt gattgagctg gatcacacat
    10321 ctcaaagcta tacattgaac cacaatgaaa ttggccgtgt acgaggtttc caaaccgaag
    10381 tggaagaaat ggaacgtcaa aatgaacaga tgattccaca gatccgtcaa cacgaaattc
    10441 cttattcaga agtgcgcacg ttctataaaa cagtcttcaa agtcttagaa gatatcgaaa
    10501 agcaacaagt agagatcgac gactcattac atgaattgag aaaaggcgaa aaagaagcgc
    10561 aagaaaagat cgatacattc gaattcaaat tgcgcagttt gaaacgtttt gtagaaaaac
    10621 aacgtttgcc tggcttgccg aatgattacc tagaattctt ttttgttgct actgaccgta
    10681 ttgaagaact aagtgttgtc ttgaataaaa ttcgagtaaa catggaagag gtcaatcgtt
    10741 tagttgctct atgtgaagaa gatctagaat tattagataa gaagacacat gatttagtag
    10801 atgcagcagc attaacggag caaatgctcc aatatgcaaa tcgttatcgt catacacacg
    10861 aagaagtgag agaagcaatt gataaaagct actatttatt caataaagaa tatcactatc
    10921 aagaagcatt agacgaaatc ggtacagcat tagagcgagt agaacctggt gctttcaaac
    10981 gcatcgaaga tttttatttt aatcatccag acttagtata aagcaaaaaa gttagtaaac
    11041 cagcaagagc aagacggatt attgtcttgt tcttgtcttt ttttgtatat ttgaaaattg
    11101 aaacaagtga aaatttcacc tgctcttgaa agcaccttgg acaaccgtta taatagaaaa
    11161 gaatttcaaa aaagaggggt ttcttatgat atattttgat aacagtgcaa caaccccaat
    11221 ctttccgcaa agcttagatg catatgtaaa aaccagtcag cggattattg ggaatccatc
    11281 aagcttgcat gatttaggca atcaagctaa ccggttgttg cagcaagcaa gaaagcagat
    11341 cgctgatttg atccatgtag aaccagaaga aatctatttt acttcaggcg gaacagaagg
    11401 agataactgg gtattaaaag gaacgatgat cgaaaaaaga gaatatggga atcatatgat
    11461 catttcagga gtcgaacatc cggccgtcag cgagacagca gaacaattaa aagaattagg
    11521 aatcgaagta agtatcgccc cagtggataa acgcggattt gtacgtgtcg acgaattgaa
    11581 agaattaata agaaaagaga cagtattagt ctctgttatg gctgtgaata atgaagtagg
    11641 atcgattcag cccatccagg aaatcagtga tcttttggat gattttccta aaattcattt
    11701 ccatgtagat gctgttcagg cgattggaaa agtaccattc tcgcaatggt tgacagaaag
    11761 agttgatttt gcaacatttt cagctcataa attccatggc ccacggggaa ctggtttcat
    11821 ttattggaaa aagggtcgtc gtttagcacc attactgaca ggtggagggc aagaaagaaa
    11881 tcagcgaagt gggacagaaa atgttccagg tattgtggct atggttaaag ctttacgttt
    11941 atcattagag ataaaggaac aaaaacctga gcagacacaa cgcttaaaag tagctctaat
    12001 caatgcgttg aagacgtatg agaaagtgac gattttttca gaaggaactg actatgcacc
    12061 gcatatttta tgttttgctc taaaaggaat tcgcggcgaa gttctcgtcc atgccttaga
    12121 agaaaaacaa atatttgttt ctactactag tgcctgctcc tcacgtaaac atatggcaag
    12181 cagtacattg cacgcaatgc atgtaccaaa tgatctggct acatctgctg ttcgaatcag
    12241 cttagatgaa aacaattcgc ttgcagaagt cgagcaattt atgattattt tcaagcagct
    12301 ttataagaaa ttttctgcaa tcaattaaaa gaggagtgtt cttgtgaaat acagtgaaat
    12361 tatggtccgt tatggggaac tttcgacaaa aggaaagaat cgaaaaagct ttatcatgca
    12421 actagcacaa aatgtcagac aagctttgaa agactttcca gaaatcaaaa tccatgctga
    12481 tcgggaccgg atgcatcttt tattgaatgg cgcagacggg cagctagtta ttccaaaact
    12541 agcaaaaata tttgggatcc aaaatttctc tccaagtatt cgtgtagaaa aagatgtcga
    12601 agttttgaaa caagctgttc aagacatcat gaaagaaatt tatactggga aagaaacatt
    12661 taaaattatc gctaaaagaa gcgatcacca atttgaattg acaagtaatg aattgaatca
    12721 aacattaggt aatgctgttt ttgatatttt cccgcatatc caagttcaaa tgcgtcaacc
    12781 agatattcca ttacgtgtag agattcggcg tgatggcgct tatctatcgt atgaaacgat
    12841 caagggagct ggaggattgc ccgtaggaac aagcggaaaa ggaatgctta tgctttctgg
    12901 ggggattgat tctcctgtag cagggtactt tgccatgaag cggggagtgg aaattgaagc
    12961 tgttcatttt gctagtccgc cgtatacaag tgaacaagca ctgcaaaaag caaaagatct
    13021 aacagccaaa ctagcacctt atgtgggaac gatccatttt atcgaagtgc catttaccga
    13081 gattcaagaa gaaatcaaaa agtcagtgcc acaaggatat tggatgacaa ttactcgtcg
    13141 aatgatgttg cgtttgaccg atgaaattcg ccgtattcgt catggtttag tcattttgaa
    13201 tggtgaatca ttaggtcaag tggcttcaca aactttgcaa agtatggtgg ctatcaatga
    13261 agtaacgaat acgccaatca ttcggcctgt agcgacaatg gataaacttg aaatcattga
    13321 agtagcagaa cagatcgata catttaatct ttctatccag ccttttgaag attgctgtac
    13381 gatttttgct cctccacagc caaaaacacg ccctcaactg gaaaaggtcc atcaatatga
    13441 agagcgttta gcaatagatg aaatgatcca acgagcacta gctggcttga agatggaaga
    13501 gatacaagca gaatcgtcta atgaaaaaac tgaagaattt tcagaattgc tataagaggt
    13561 tatgaaaata gtgttttgac cagagtatta gagaaacaaa acgaaaaaat agctccatgt
    13621 attttaccgt ttttgaacta atgtcgaaga ttagctgatt gaataccatt tatcaagaag
    13681 ttatggaaaa gccggttctt ttttactaac tgaaaaataa aattttattt agagatcaag
    13741 ctacagttga taaatgatat aaaaaagaga atttcccatg aaatagcgag gttgtgacag
    13801 aagtgggcag cttcaagaaa taagaaggaa tttacgaaaa ttgcttttca aatttttgtg
    13861 aaatttcggc ttatttttcg aagaagttac ttctggaaca ccgtgtatca gaggttgcga
    13921 aaaagctgcc ctgcatcaag taataagaac agccaaacaa aaatagttcc tcatatttta
    13981 cgtttggttg agcttattac cgcagatgca agcttttgaa caccgtttta tttgtctcac
    14041 acccttttcc ttcctacttg taaccttgct cccgtgttag aaacatgtta cactaaatat
    14101 gtgaaattat taacaaggag cgtcgttatg cgagaaaaaa cagatacact gccaaaagca
    14161 actgcgaaaa gattaccttt atatttacgc tacttgaaga tgctggatga ctcaggtata
    14221 agtagaatca aatcaaatga gttcagcgaa atcacccaaa ttccttctgc aacgatccgc
    14281 agagacttct cgcaattagg cgagctaggc agaagcggat atggctatga tgtccctttt
    14341 cttatcgatg tattcaataa tatccttaat actaaagaag aaaaacgaat cgcattagta
    14401 ggatatggaa atcttggcaa agcattgaaa cacaacaatt ttagaagaaa tgaaaatcta
    14461 aatatcgtgt gcgtctttga taatgatcca gcactgatca atcgagtaat tgatggagaa
    14521 atgatctatc cgattgatcg tttcgcggag atagccaaag caaaaaatgt gacagtagca
    14581 atctctacgg taccaagtaa atattcacaa tctgctattg atgaaattgt caaaggaaat
    14641 gtaaccgcta tacttaattt tgcgcctgac agagtaactg tcccagctta tgtcaatgtt
    14701 caatacattg atttgacaac cgagttgcag acgcttattt actttgatga gaattactca
    14761 gaagtttttt cttaggaaaa tttgagtagg cttattgatc ttgcgagaac tcgtcgtaat
    14821 tctttcaaaa attttttagt actaaatgaa gtggtttttt cacgaccact tgttgactaa
    14881 aaaaaaaaac cattgtagaa tgaagcataa cgctgaacaa gaggagtaac tggcacattc
    14941 attcttagag agagaactat ctgctgaaag gttctcaatg acaaccagtg aaggtagctt
    15001 gggagtttct gtcttgaatg gagtagagat agacgagtcg tgatcgttaa ctcacttgag
    15061 aggcaataat gattgattat tgcaatttag gtggtaccgc gtaaatcata cgtcctagaa
    15121 tacgaaagta ttttaggacg ttttttagtt taaaggagga aaacagcatg acggaagaaa
    15181 ataacctatc aacaaaatat aatccgcaag aagttgaagc tggacgttat cagcattggt
    15241 tagaacaaga tttgtttaaa ccgagcggag ataaaaaagc aaaaccttat tcaatcgtaa
    15301 tcccgccccc aaatgttact ggaaaacttc atttagggca tgcttgggat acgaccttgc
    15361 aagatatgct gattcgtcaa aagagaatgc aaggatatga tactttatgg ctgcctggta
    15421 tggaccatgc aggaatcgcg acacaagcaa aagtagaagc gaagcttaga gagcaaggga
    15481 tttctcgtta tgatctaggt cgtgaaaagt tcatcgatca agtgtgggaa tggaaagaag
    15541 agtatgcgag ccacatccgc gaacagtggg ctaaaatggg cttatcttta gattacagta
    15601 gggaacgttt tacattagat gatggattat ctgaagcagt acgtaaagtt tttgttacct
    15661 tatacgaaaa aggtttgatt tatcgtggtg aatatatcat caactgggat cctcaagcac
    15721 gaacagctct atctgatatc gaagttattc ataaagatat cgaaggcgcc ttttatcata
    15781 tgagctatga actagcagat ggatcaggcg tagtagaaat tgcgacaacc cgtccagaaa
    15841 cgatgttggg agatacggcg attgctgttc atccagaaga cgagcgttat caagcgctca
    15901 tcggcaaaaa agtgattcta cctttagtca ataaacaaat cccaatcatt gcagatgaat
    15961 atgtagatat ggaattcggg acaggtgtag taaaaatcac tccagcacat gatccaaacg
    16021 actttgaggt aggtaatcgt catgatcttc cacgcgtaaa tgttatggat gatgatggta
    16081 cgatgaatga actagcagga aaatatgctg gaatggaacg ttttgctgct cgtaaagcaa
    16141 tcgtgcaaga tctgaaagaa atcggtcgat tgattaaaat cgatacgatg atccacagtg
    16201 tgggtcattc cgagcggaca ggtgtcgttg ttgaacctcg gctgtctact caatggtttg
    16261 taaaaatggc gccgttagcg aaaaaagcca ttgataacca agaaacagat caagcagtgg
    16321 aattcttccc tccaagattc aatcaaacgt ttttacgttg gatggaaaat gtccatgatt
    16381 gggtaatctc acgacaatta tggtggggac atcaaattcc tgcatggtat cacaaagaaa
    16441 caggtgaaat gtatgtaggg atggaagcac cagcggatag tgaaaactgg gtacaagatc
    16501 ctgatgtttt agacacttgg tttagttcag ctttgtggcc tttctcaaca atgggctggc
    16561 cagatacgga aagcgaagat tataagcgat acttccctac tagcacttta gtaaccggtt
    16621 atgacatcat tttcttctgg gtaagcagaa tgattttcca aagcttggaa tttactggcg
    16681 aacgtccatt tgagaatgtc ttgattcatg gattgattcg tgacgaacaa gggcggaaga
    16741 tgagtaaatc attaggtaat ggaatcgatc cgatggaagt aatcgaaaag tatggagcag
    16801 atgctctccg ctggttctta tcgaatggtt ctgcaccagg acaagatgtc cgcttcagct
    16861 atgaaaaaat ggatgcttct tggaacttta tcaacaaaat ctggaatgct tcacgtttcg
    16921 ttatcatgaa tatcgaagga atgaccgtag aagagatcga tttatctgga gaaaaatcag
    16981 tggctgaccg gtggatcctt actcgtttga atgaaacgat cgaacgagta actgagctat
    17041 ttgatcgttt tgaatttggt gaagcaggtc gacaactgta taactttatc tgggatgatt
    17101 tttgtgactg gtacatcgaa atgagtaaag aagtacttta tggagatgat gctgtttcaa
    17161 aacaaaccac ccgtagcatc ttagtccata cacttgatca gatcctacgt ttattgcatc
    17221 caatcatgcc attcgttaca gaagaaatat gggaacatat tcctcatcag ggaaattctc
    17281 tagtagtggc agaatatcct gttgtacgtc cagagtttga tgatgaaaca gcttctaaag
    17341 ggatggaagt attgaaagaa ttgattcgag ccgttcgaaa tattcgttca gaagtgaata
    17401 caccattgtc taaaccaatt acgttgctaa ttaaaacaaa cgatgctaaa atcgatcaat
    17461 ttttagttga aaatacaagc tatatcgaac gcttctgtaa tcccgaagag ttgacgatat
    17521 caagtgagat cactgcgcca gatctagcga tgtcagctgt tctgacaggt gcagaaatct
    17581 tcttgccgtt agcaggattg atcaatattg aagaagaaat caaacgatta gaaaaagaac
    17641 tggaaaagtg gacaagcgaa gtaaaacgtg tccaaggaaa actttctaat gaacgttttg
    17701 tttctaacgc gccggaagaa gtagtagagg cagagcgtgc aaaagaaaaa gattatttag
    17761 agaagcaaac ggctgtcaaa gaacgaatcg aaagtttgcg tactattcaa taagaataga
    17821 aaaagagagt gtgaaatcag ctagctatta ctaaaagcta gctgattgaa catcgttaca
    17881 ggaaagagtt tgagcctcac tcttagcttc gattcataag gagatgtgac ataaattttg
    17941 tcgcatctct tttttagtct actcttatta tgtaggagaa ggagggaggt ggatcatgta
    18001 tcaagagtca ataaagtagt tgataaaatc gttcctctct ctgtttcttt tctctttgtc
    18061 atacttttat ttttcttatg tgttctattt gttataataa aagagaaatg ggggaattgt
    18121 tgatgacgat tgaagaagca atagaatgga tccatagtcg attaccattt ggttcacgtc
    18181 ctggccttga ccgagtcgaa gctttattag aagtggtagg gcatccagag agaaaagtaa
    18241 aaacgatcca tatcgctgga acaaacggaa aaggttctac tgtagcttat ttgagaagcc
    18301 tattagaaga ggctgggctg acagtaggta catttacttc accttatatc gaaacgttca
    18361 atgaacggat cgcaatcaat ggcaaaagca ttccagacaa tgatttgatc cagcttgtaa
    18421 aaaaatatca gccactgatc aaacaacttg atcaatttga agcagtagct ggtattactg
    18481 aattcgagac attaacagca atggcatttg attatttttt gatgaagaaa gtggacgtag
    18541 cgattatcga agttggctta ggcggcttat tggattctac caatgtagct gtaccaatgc
    18601 tgacgggaat aacaacgatt ggacttgatc ataccgaaat acttggagat acaattgaag
    18661 aaatcgctgc acaaaaagct ggaatcatca aacaaggcgt tccagtagta acaggaaata
    18721 ttgccccaga agcttttact gtcatccaac aaacagctaa agcaaaaaat gcaccgcttt
    18781 atgtatacgg ggaaaattat aaaataaact atctgcatcc tgatgagatg tggggagaag
    18841 tatttgaatt ttttaatgaa ataggaaaga tccctcaatt aaccactccg cttttagggc
    18901 aacatcaagc tgaaaatgca ggagtagcca tcgaattgta ttttgtttac tgtcaattgg
    18961 aaaagctgcc tttccgagaa aaagatgttt tgaagggatt gaagaaatct cgatggccag
    19021 gaagaatgga gcgtttgagt aacgagccat tagttgtttt agatggtgcc cataatacgc
    19081 atgcaatgaa ccggcttgtt gagaatttga aaaaagaatt caaaggatat caaatcaaca
    19141 ttttgttttc cgccttacga actaaaaatg ttagagaaat gctagaacga ttgcagcaag
    19201 taccaaatgt ccatatttat ttgacgacct ttgaacatcc aaaagccatc gaattaagtg
    19261 aagtatcaga tttagcaagt gagagagtat caatcgtttc attgtggcaa ttcggattag
    19321 cagatatatt ggaaaagatg acaaatgaag acgtattagt tgtgaccggt tcactttatt
    19381 tcatttcaga agttcgccat ttattaatac aattaggagg agatcatgaa gtataatgca
    19441 gtgatttttg acatggatgg tttgctgttc gacacagagt tagtgtatta cgaagccaca
    19501 caagtagtag cagacaagat gggttttcct tatgataaag acctgtattt aaaattctta
    19561 ggtgtttcag atgaagaagt atgggcgaat tatcatatga ttttctcgga atttggcaaa
    19621 acaagtgtac aaagattcat agatgattca taccaggaaa cgcttgaacg tttttcgcaa
    19681 ggagcggtac aaatgaagcc cggtgtgttg gaactcctct cttttttaga agaacatcag
    19741 attcccaaag ttgtcgcttc cagtaaccaa cgagcagtca ttgaactttt attgaagaag
    19801 aacaatctgt ttgaacgatt tgagacgatt gtttctgcag aaaatgttca acgagcaaaa
    19861 ccagacctag aaattttctt gttagctaat cagaagctag gtacagcgaa gaaagaaaca
    19921 ttgattttag aagattcaca aaatggtatt ttagcagctt ctgcggctga gatcccggta
    19981 gtcatggtac cagatctgct tccaccatcc caagaattga tcaaaaaaac agctggggtt
    20041 gtttcttctt tacatgaaat ccctgcattg ttaaaataaa aatccatctc ttttcgtatc
    20101 ttattttaga taagatagaa aggagatgtt tttttgggaa aacgattgat caaagaagta
    20161 cctactagct ctttaccaag ggaacgtatg gaaatttatg gcgcggaagc tttatcggat
    20221 caggagcttt tggcaatctt actgcgtacc ggtcaacacc cttatagtgt gatgtcgatt
    20281 gctggaaatc tgttaaaaac gtttggagga ctggcctctt tacgacaggc tactttgcat
    20341 gagttagaag agattcgagg gataggacgc gtgaaagcaa tcgaaatcaa agccttgatc
    20401 gaattaggtc gacgaattca gacggatcat gaaagagcgg ctccaccggt tcgtaccagc
    20461 tacgaattgg cccagcaatt gattctggaa atgaaagatc acaaacaaga acatttggtt
    20521 tgtatttatt tggacacgaa gaatcaagtg atcttaaaaa aaacagtgtt catcggttcg
    20581 ctgaatcagt cagtcgctca tcctagagaa attttccatt atgctgtacg ttattgtgca
    20641 gcaaggatcg ttttagcgca taatcatcct tctggtaatg ttttaaccat agtataataa
    20701 ataatagtag ttaaagtagt ttaagaggag atattaaagg gatattttac tttaaataaa
    20761 aattaacaag gtttataatt tgtagtagtt aaagtattta aagaggtggg aacatgtgaa
    20821 ggttcaaagg atagaagtgg agaataagcc gtatccattg tatttattac tagataaaga
    20881 ataccagcta atagaaccag taatgaaatt tattaaatac ttagataata ctggtaagtc
    20941 tcctaatacc attaaagcat actgctatca tttaaagttg ctgtacgagt tcatggaaca
    21001 gagaggtgtt attcttaatg atattaactt tgagttgtta gcagacttcg taggttggtt
    21061 gagatatcct tcagcatcaa atgtaattga tcttcagtca aaaaaagcca taagagaaga
    21121 aacgacagtg aatacaattt taaatgtagt tatgagtttt cttgattatt taagtagatt
    21181 aggagaattt aaatcaattg atgtatttaa acaagcaaag ggaagaaatt tcaaaggatt
    21241 tttacatcat gttaataagg gtagatacca aaagaatgtc ttaaagttaa gggttaaaaa
    21301 gaaacagata agaacattga gatcagagga agttaagcaa attattgatg cttgtcatac
    21361 gaaaagagat aaattaattt taatgcttat gtatgagggt ggtttaagaa tcggtgaagt
    21421 gttatcgctt aggcttgaag atattgtcac ttgggacaat caaatccatt taacacctag
    21481 agatgttaat gttaatgaag cttatattaa attaaggaag gaaagaacaa tacatgtgag
    21541 taaagaactt atgtcacttt atacagatta cttgatatat gagtatagtg aggaattgga
    21601 gcatgattat gtttttattt ccttaaaaga aggctatttt gggaaaccac taaagtacca
    21661 aagtgttctt gatctagtta gaagaatagt taaaaggact ggaatagaat ttacatcaca
    21721 tatgcttcgc cacactcacg caacgcagct aattagggaa ggatgggatg ttgcgttcgt
    21781 tcaaaagaga ttaggtcacg cacatgttca gacaacgtta aatacctatg ttcatctttc
    21841 agatcaggat atgaaaaatg agtttaataa atacctcgag agaaaggagc ataagaaatg
    21901 aatgcttcta gtaaaaggaa aattattagt cagagtgaga ttagcaaaaa aatagctgta
    21961 atgaatgaag aaatgcaggg gttttgggct aataatagtt gggatataag aaaatgtcca
    22021 catccttctg ccatagaatt aagtaagaat cctgctttaa ggaatcgttg ggttcgtttt
    22081 gaacgtgtta aaaatctgtg gttaagaaca gaattgaaat atttttattt ttaccattta
    22141 aacaatggaa tatggaatgc aaaaactgtc tggattagaa aaggaacagt aattaataaa
    22201 atgttagatt tcttagattt aaagtatcct agcattactt caattactga agttcctatt
    22261 gaaaaagcaa tgacggagta tagaacttat ttgacaaaac ggggtgttag aattaccact
    22321 actaattata agattactgc taatcaagaa aaaacacctg taaaagctaa ttcctactat
    22381 gttactaatc taaaacaatt tatggagttt tatgagaact tttattttga tggagaggag
    22441 tgggataaag acgtttggga tagacgtaac ttacctttgc cagatgataa ggttaaccca
    22501 acacaatatg aatatacaat taactttaaa gggtttcgga atacatattt taaacaactt
    22561 gtaaaaagat attgtaagtt gagattgaac atggatagct tttcctatgt aagtgatatt
    22621 gcccaaagac ttaaagagtt ctttaatttt ctggacatga aatttaaaca agttcagaga
    22681 gtacaccaat taacgagagt ggaaattgaa gcatatttaa gtgaactaaa catgatggga
    22741 ataaaaccta gtacaataac tgggaggatc tctatattgg aaggactatt tagtaccctt
    22801 cttaggctag aatgggatga tgttccttcc aaaatattaa tttattctga ggactatccg
    22861 aaaataccaa gagcaaaacc acgctttata gatgaattcg tcctagagca attgaacagt
    22921 catcttgata aattacccga atatatagct acgatgacta tgattgttca agaatgtgga
    22981 atgaggataa gtgaattgtg caccttgaaa aaaggctgtc tattagagga caaagatgga
    23041 gatttctttt taaagtatta tcaatggaaa atgaaaaagg agcatatagt tccaatatct
    23101 aaagaggtag ctttacttat taaagttcgg gaagataaag tttcagagga atttccagat
    23161 agtgaatacc tctttccaag aaaagatgga tcgccattaa aacaagaaac atttagaggt
    23221 gagttaaata aattagctta tgagcaaaat atagtggata aatcaggtga gatttataga
    23281 ttccatgccc atgcctttcg ccatacagta ggaacaagaa tgattaacaa cgggatgccc
    23341 cagcatattg tgcagaaatt tttggggcat gaaagcccag aaatgacaag cagatacgct
    23401 catatctttg atgaaactct aaaaaatgaa tttactaaat ttcaggaaaa actggttacc
    23461 aataatggag atgtgcttga tctagatgaa gataatgaag tcgatgatgt agagcttcaa
    23521 tggttcaaga aaaatataaa tgcacaagtg cttccaaatg gttattgtag attgccagta
    23581 gtagcaggtg gttgtccaca tgcgaatgca tgcttagatt gcactcactt ctgtaccagt
    23641 aagcaattct taccacagca cgaagaacag ttagagcgta cagaagagtt attagccata
    23701 gctaaggata aacaatggca aagacaagta gagactaata gccgtgttaa agagcgttta
    23761 gaacaaatca ttggaagttt gacggggtaa ttatcaatgg ataaacaagt tagaaataca
    23821 acagaaattg tacgtttggc gaagcagaaa tcaaaaaaga caagggaaaa agtagacaaa
    23881 gcgatttcta aattttcgat tgaaggtaaa gttattaatt ttaattcaat agcaaaggaa
    23941 gctaatgttt ctaaatcatg gctttataag gaacacgata ttaggcaaag aatcgaatcc
    24001 cttcgtgagc gtcaaataac agcaaatgta gtctcaaaac ccaagaaaag ttctcgttcg
    24061 gaggaaatcc ttattaaaac cttaaaaaga agagtaatgg aattagaaaa agaaaataaa
    24121 aaattacaga accaaattca aaaattatat ggagatctgt ataataaaga ataattatta
    24181 atctgtagac aaattgtgaa aggatgtact taaacgctaa cggtcagctt tattgaacag
    24241 tcatttaggt atatgtccaa tctagggtaa gtaaattgag tatcaatata aactttatat
    24301 gaacataatc agttacgagg tgaaatcatg agcaatttga ttaacggaaa aataccaaat
    24361 caagcgattc aaacattaaa aatcgtaaaa gatttatttg gaagttcaat agttggagta
    24421 tatctatttg gttcagcagt aaatggtggt ttacgcatta acagcgatgt agatgttcta
    24481 gtcgtcgtga atcatagttt acctcaatta actcgaaaaa aactaacaga aagactaatg
    24541 actatatcag gaaagattgg aaatacggat tctgttagac cacttgaagt tacggttata
    24601 aataggagtg aagttgtccc ttggcaatat cctccaaaaa gagaatttat atacggtgag
    24661 tggctcaggg gtgaatttga gaatggacaa attcaggaac caagctatga tcctgatttg
    24721 gctattgttt tagcacaagc aagaaagaat agtatttctc tatttggtcc tgattcttca
    24781 agtatacttg tctccgtacc tttgacagat attcgaagag caattaagga ttctttgcca
    24841 gaactaattg aggggataaa aggtgatgag cgtaatgtaa ttttaaccct agctcgaatg
    24901 tggcaaacag tgactactgg tgaaattacc tcgaaagatg tcgctgcaga atgggctata
    24961 cctcttttac ctaaagagca tgtaacttta ctggatatag ctagaaaagg ctatcgggga
    25021 gagtgtgatg ataagtggga aggactatat tcaaaggtga aagcactcgt taagtatatg
    25081 aaaaattcta tagaaacttc tctcaattag gctaatttta ttgcaataac aggtgcttac
    25141 ttttaaaact actgatttat tgataaatat tgaacaattt ttgggaagaa taaagcgtcc
    25201 tcttgtgaaa ttagagaacg ctttattact ttaatttagt gaaacaattt gtaactattg
    25261 aaaatagaaa gaaattgttc cttcgatagt ttattaatat tagtgacatt tgcatgcttc
    25321 aaagcctgtc ggaattggtt tttagtgaaa agaacacgat attcacggtt tacccactta
    25381 taaacaaaag atcgatactt tttgtagtcc ttctttgaaa tcaatggttg atgtcgttca
    25441 agaacaatca atacagagtc tacacttggc ttaggatgaa aatatagtgg tggtactttt
    25501 ttgagcattt ttatatccat ctccaccatt aatagtaaac ccaaagctcg ttgcagattt
    25561 tgcaatcttt tcgcaaatcc cttctcaacg ataagatagc tatatttagc ctgactttca
    25621 aaggtaattc ttttgacaat atccgtactg atgttataag gaatattacc atatatctta
    25681 tagtttatat gttttgggaa ggaaaatttt agaatatccg tttgaatcac ttttatattc
    25741 tcagaggggt ttaccgcttc tttagtcact tgacataagc ctccatcaat ttctatagca
    25801 gtaactgatc gactcatttt gactagctct ttggtaaaat gtccttttcc tgatccgatt
    25861 tctattacgt tgtcttgttt actgatattc gtgtgattca atatttcttt tacatgcttt
    25921 ttagaagtaa taaaattttg cgtgtcttta gggtttttct ggttcattat aaccttctcc
    25981 ttactggtta taatgaactg acattaagcg ttcattataa ccaattaatt ttgatttggt
    26041 tgataacgaa ctttattaat aacaaatata gaaaaagtac ccatactttt atctcctttc
    26101 catttttttc attatgaatg agataaagta atttctacta ctgcgatact ggtgcacata
    26161 attgaaacct ccttatgtaa ctgaatatga ttgtatcatc ttttcatttt agaataaacc
    26221 tttatatgta attttgcact tgacacttca gatggacgtt ttcttcaaaa taatatgttt
    26281 tgaaacttga taagagaagc cactgtcttt actaaaaagt aaaggctgcg ataaaggaca
    26341 gtgactttaa taaagttgca ctgttgtatt gtctttggat aaaattgtct aaagggaagt
    26401 tttatcccca acgaaaaaac atctaaagtg gtaagtaatg aaagaatgtc gataaagtag
    26461 gaatggggga tctttttgaa tacaatagaa tcacagtttg ataaggttgc agaagattac
    26521 gatttcgtga atgagctttt gaatgattat tcattctttg tgtctaatat gtctccaaag
    26581 aaaggcagag cattagatat cggatgtggc tcgggtttgt tagtggagaa attagcaagt
    26641 tattatgatg aagtggtagg gattgatatt tctaatcaaa tgctcgatct tgccaaatct
    26701 aaacgtcaac taacaaatac ggtctatctg aatatgaatg cagaacaact taattttaat
    26761 gagaagtttg attttattgt aagccgaaca acctttcatc atttggatga tatagccagc
    26821 gtaatacagc aaatgaagga actgttgaat gaggaaggaa gaatagttat tcttgataat
    26881 gtatctgaag tcgagacacc gcctacctat gtttataaat taggggcaat ccaagagttt
    26941 ttaccgcatt gtttcaaatt tggaataaaa aatgcgatta gaatttataa tcacaacaca
    27001 tctaaatcat ggcttgagca tttagcttca gataagtacc tatctgaaca aaattattac
    27061 gatttatatg aaaagttgtt gcctggatgc caattccata aaatgggttg ggcaatgggg
    27121 gttgtctgga caaaataact taaagctgtg gctttgaaaa gttgaatttg tgattgtttt
    27181 cagagctatc gggtgcttta gtataaataa caatgaataa aaaatcagcc tgtatcttat
    27241 gttactatta agtaatttaa gatacaggtt ttactatttt tgaaataagt accttccact
    27301 tcaaatacat gtattcaaag tgaaagtatt gatttaaatg attcttttgt aatttactct
    27361 tgataatatg aaattccatc gcagcaggat atgaatttta ccaaaaggat acaaaagtgt
    27421 ggagaaatga tggggatcac tgtactggac cacttgatca tcggacgcaa acgatatttt
    27481 agtttgagag aagaaggaat gatggaagag aagtaagttg ctttcttgca aaaaaaatga
    27541 agcaagtgta caattatatt gtaattttgt ttgggaatag aagttgaaac atctgttgca
    27601 cctctatcgt acacgagcaa tgttacaatt taaattagtg cttttggcac gaggaggata
    27661 ttattatgga aaacggaaca gtaaaatggt tcaacgctga aaaaggattt ggatttatct
    27721 cacgtgaaga tggaagcgac gtattcgttc acttctcagc tatccaaggt gacggattca
    27781 aaacattaga agaaggtcaa gcagtgactt tcgacgttga agaatcagac cgcggcccac
    27841 aagctgcaaa cgttgtaaaa gcttaatttt atctgtaaat caaattgatg gtagacatca
    27901 atttttacga gagaacaaag cgagtaacaa aaatacctgc cacaagagac aatttctctt
    27961 gtggcaggta tttttttgta tgaaaaagta aataaattta agaggctatg aaagagccac
    28021 cttgtatcaa ggaataagaa caaacaaaaa tggcttctca tgtttttcat ttgttttctc
    28081 ttatattcag gacaaggtgc tgatttcata acctcttatt tattcttcat ctggtgtcat
    28141 taccattggc aaaatcattg gatgtctttc tgtacgttcg aataagaaag gctgaagcgc
    28201 gccagtcatc gcttctttca atttatattc tgaacagtca ggtccattca aacattctct
    28261 taaagcgtgg aataagattc gctgagcttc attgatcatt tctcctgatt ctcgcatgta
    28321 gacaaatcca cgagagagaa tatctggacc agccaagatt tcttttttct tgatatccac
    28381 tgtcgcgaca gctaatacaa gaccttcttc tgaaagcagg tgacggtcac gtaaaacgac
    28441 attaccaata tcaccgatcc cgctaccatc gatatacaca tctccagcca caaaatgccc
    28501 agcaggacgc gcagaatcgc tagttaacgc taaaacatcg ccattttcca tgatgaagca
    28561 gttttctgca ggaacaccta catcttgtgc tagagaagca tgtactttca acatgcggaa
    28621 ttctccgtgg acaggaataa aatattttgg cttcatcaaa cgaagcatta atttttgttc
    28681 ttcttgtcca ccgtgaccgg aagtatggat gttattgatt tttccgtgaa tcacttctgc
    28741 accagcttca gacagcaaat tgatcaaatg attgacactt gttgtattac caggaatagg
    28801 tgaacttgaa aagatcactg tatcgtctgg ctggatttgg atttgacggt gagtaccatt
    28861 cgcgatgcgg ctcaacgcgg ccatcgcttc tccttgagaa cctgtacata ggatcatcgt
    28921 ttcattggca ggcaagcgat tgatttcgtg tgcatctaca aatgtgccgt ctggaacttt
    28981 gatatatcct aaacgttgac cgttgacgat ggcattttcc atgcttcgtc cgaaaacagc
    29041 gattttacga ccagttgcca cagcggcatc tgctgcttgc tgcaaacgga agatgttaga
    29101 agcaaagctg gcaaaaatga tacgtccttc gatttttgaa aagatcttca aaatagaatg
    29161 cccaatcgtt ttttctgatt tagtaaatgt tggaatctca gcatttgtac tatcagataa
    29221 aaggcacaag actccttctt ccccgatacg agccatttta tgcaagtttg ctggttcgcc
    29281 aacaggagtg aaatcgaatt tgaagtctcc agtcgctacg atatttccag aaggtgtttt
    29341 caccacgatc cctaatgcat ctgggatact atgggtcgtt ctgaaaaagc tgacagaagt
    29401 ctttctaaaa cggatgatcg tatcttcatt gatctcatgg agctctgcat ctcgaagcaa
    29461 gccatgctca tctaatttgt ttgtgatcaa cgctaatgcg agaggaccag catagatagg
    29521 aatatttgct tgtttcagca agtagggtat tccgccgatg tggtcttcgt gcccgtgagt
    29581 aatcaccaag gcttttactt tgtgtaagtt ttgtatgatg tagctgtagt cgggaatgac
    29641 atagtcaatt ccaagtaaat cgtcttccgg gaatttgatc ccggcatcaa tcaaaatgat
    29701 ttcatcttgg aattgtaccc catacgtatt tttcccgatt tcacctaatc caccaatagc
    29761 aaagacgcct gtctcgttat ttttgatgga aagtttcata ttaaaactcc actaatgaga
    29821 aatcagcatt ttctttttcg tactctagat gacttccttc tagaagttga acgaattcaa
    29881 tattgtatgg ggtattttct tccacacgtt ggcgagcgtc tacatcgctt tttgcctcaa
    29941 tgtaaagaga ttttgtttct tcccgtttag ggttgcgtac ttttgtttct tgataataaa
    30001 ctttatagat cattgttttg tctcctttat ctgcgtaaag cagtgattta gctagttgat
    30061 ttttttagac aacacaaaac gaggggacaa gccacctggt ttttcatatg ctttgactag
    30121 aatactggat tcgagtcaaa gagacaagcc tcgtttttgc ttatctgttc ggatattttt
    30181 tcaaccgtac cttctgtata aaaaatatac aacaattaat tgttattcta gcataaaaca
    30241 gcaaataagt atagaaatca actagattga aagaaaaaat caatgtttat catcccgcta
    30301 taatcataat gactttttta tgagagaagt gttctattaa taggctcgtt gtgctatcat
    30361 ttttagtaat gaaaatgtaa caaaagatta aaaggagatg agaaagtgaa actaatgtgg
    30421 cattatacca tgcgttataa gaaattcttg tttttcaatt tcatatgcgt atttggtttt
    30481 attttgattg aattaggact tcctacgatt ttagcacgta tgatcgatgt aggaatccgc
    30541 aatgatgatt ttgattatgt gaagcaacaa gggatattta tgatcgttat tactgtaatc
    30601 ggtattattt tgaatatctt gttaggttat tttggttcga ggattacgac taatatcgta
    30661 gcagatattc gagatgattt gttcgaaaag atccaaagtt attcgcatca ggaatacgaa
    30721 aatctaggcg tatcttcatt gatcactcgt acaacaaacg atgcttatca gatcatgttg
    30781 ttcctgcaaa atattttacg tatcggtttt atggcaccaa tgatgttcat tgtcagtttg
    30841 tacatggtca tgcgtacaag tgtgacactc agtttgtatg tagtaggtgc attaccttta
    30901 ctattattgg cagtcgtagc gattgctaag gtgtctgaac cgttatctaa aaagcaacaa
    30961 aaaaacttag ataaaatcaa cagtatcttg cgggaaaatc tttcaggatt gcgtgtgatt
    31021 cgtgcattcg tcaatgaaaa gtttgaagaa aaacgttttg ctgtagtcaa tgagaattat
    31081 gcttctagtt caaaaagctt attccgtttg atggctgttg cacaaccggg tttttatttc
    31141 ttatttaata tcgtaatggt attgatcatc tggagcgggg cagtgcaaat cgatgcaggc
    31201 gagcttcgtg taggagattt gattgcgttt atcgagtata tattccatgc cttgttctca
    31261 ttcatgttat ttgctagtgt gttcatgatg tatccaagag cagctgtttc agctggacgt
    31321 atccaagaag ccctagatgc caaaccttcg attgtagaag atccaaatgg tatatctgat
    31381 acaaaaacta aagggtatat cgaatttcaa aatgtaacat ttgcttatcc aggccattcg
    31441 caagagccag tgatacgaaa tgtcagtttt acagcctctc caggagagac cgttgctttt
    31501 attggaagta ccggtagcgg aaaatctacg ttgatccaat tgatccctcg cttttatgat
    31561 gtgagtgaag gaaagatctt gatcgatgga gtagacgtga gagattatcg gctaagcaag
    31621 ttgcgagata agattgggtt tattccgcaa aaagcgctgc tatttactgg aacgattgca
    31681 gataatttgc gatacgggaa agaagatgct acgcaagaag aaatggaacg tgcagctgag
    31741 attgctcaag cttcagaatt catttctcga aaaccagatg gatttaatga acatttatca
    31801 gaaggtggtt ccaatttttc aggtggacaa aaacaacgat tggcaatagc cagagccatc
    31861 atacgccgtc cggaaatcta tatttttgat gacagctttt ctgcattaga ttatcaaacg
    31921 gatgcgaagt tacgtgctcg tctgaaaaaa gaaacgacag aatctactgt attgatcgta
    31981 gcacaacgtg tgggaacgat catgcatgca gataagatca tcgtattaaa tgaaggagaa
    32041 gtcgtcggta tggggacgca tcgggagttg ttggaaaaat gcccgattta ttatgatatt
    32101 gccgcttctc aattatcaaa ggaggaatta gcatgaaaaa aaccttttct tccttaaaac
    32161 gtttagctcg atatatcaga ccgtatcgtg agacttttct tttagtgatc ctatttacag
    32221 ttctgacagt ggctttcaac gtggctttgc catatgttac aggacttcct actacagaaa
    32281 tcagccgtaa tattgccaat ggcgaatcaa tcaatttttc ttatatattc aaatgtctca
    32341 tttggatcac agttgttgga atagggtatt gtatttcgca attactttct ggtgttttga
    32401 tgacaaatgt cgttcaaagt gccatgcatg atttgagaag agacatagaa gaaaaaatca
    32461 atcgtcttcc ggtttcatat ttcgataaaa accagcaagg aaatattttg tctcgtgtga
    32521 cgaatgatgt agatgcagtc agcggtgctt tacaacaagc ttttattggt atcgtaaatg
    32581 cgatattggg aattacgttg gctgcagcaa tgatgtttta tatccagccg atgatggcgc
    32641 tgatctctat gatcatgatt ccaacatcga tctggatctc aaaacgagtg atcaatgctt
    32701 cgcagaaata ttttcaaagc atgcagaatt ctcttggtga gctaaacggc tacgtccaag
    32761 aaaatatgac aggttttagt gtattgaaag tatatggacg cgaaaaagaa acattggaag
    32821 gattcaaaaa agttaatcac agcttgaaat actttgggtt tcgtgcagca ttcatttctg
    32881 gcttgatgat gcctctagtt caattaactg cctatgctac ttatattggt atggctgttc
    32941 taggaagctt ttatgttatt actggggcga tcgttgtcgg tcaactacaa gcattcatcc
    33001 aatatatttg gcaaatcagt cagccaatgg ggaacgtgac acaattgtct tcagttttgc
    33061 aaagtgcatc agcggcaacg aaacgtgtat tcgagatttt agatgagcca gaagaaaaag
    33121 aaaacaaagt agatgcttca ctgccagaaa cgattttagg agatgtgaca ttcgatcatg
    33181 tcgattttgc atatgatccg aagaaaccat taatccaaga tttgaatttt gaagtgaaag
    33241 ctggtcaaac cgtagctgtg gtcggtccga ccggtgccgg caagacgaca ttgatcaatc
    33301 tattgatgcg tttttatgac gtagacaaag gagcaataag gattgatgga atcgacacca
    33361 aatcaatgag tcgcagtgat gtgcgttcgc tatttggtat ggtcttgcaa gatgcatggc
    33421 tttatcaagg aacgattgca gataatatcc gcttcggaaa gcttgatgcg acagattatg
    33481 aagtagttga tgctgcgaaa acagcaaacg tagatcattt tatccgaacg atgccagatg
    33541 gatatgatat ggccatcaat tctgagggag acaatgtgtc tttaggacaa aaacagttgc
    33601 tgacgattgc ccgagcagtg gtctccgatc ctaaaatctt gattttagat gaagcaacaa
    33661 gttcagtaga cacccgttta gaagcgttga ttcaaaaagc aatggatcgc gtgatggagg
    33721 gacggacaag tttcgtcatc gctcaccgtt tatcgacaat ccgtgatgca gatctgattc
    33781 tagtgatgga tcaaggtcag atcattgaaa aaggaacgca tgagagttta ttggcacaag
    33841 gtggatttta tgaaaaactg tataacagcc aatttgctga ggaagcagaa taagtcatat
    33901 aagaataaaa cagtttaaaa cttatttttt gttctctatt cttgataatg gtaaagtaaa
    33961 gttatcaaga aaagtgagga cacggagatg gaacagtttt taaatgaaaa aattactgaa
    34021 gccaaaagag aagttgatcg tttaacgcac gtctgtcaga cgaagccttt tgattcagct
    34081 gatttttttg aaaatgagtt tcaaatcgaa cgcttggcag cacaaataga agtgtacgaa
    34141 ttaattttaa aaaaagtaat agaagaaaat atagatgtac aataaaaaca ggtaaaacgt
    34201 cagttaccag atactgtaaa tagacatctg gcctgagcag gttttacctg ttttttttct
    34261 tatgaaataa atatatgcta tgataccttt attcaatagg attagtttct acaaaaaatg
    34321 aaaggagaaa aataaatatg gaacggttta tagcatcaaa tgccacagtg attggtgatg
    34381 tgacattgtc agaagatgtg acgatctggt atcaagctgt tttacgcggg gacagtaatt
    34441 ggataaaaat aggacaaaga acgaatatcc aagatggtac aatcatccat gtcgatcatg
    34501 atgcacctgt agatattgcg gagaatgtga cggtaggaca tcagtgcatg ctacatggct
    34561 gtacaatcga aaaaggggcg ctgatcggga tggggaccac gatattgaat catgcggtta
    34621 ttggtgagaa tagtttgatt ggtgctggat cattagtaac agaaggaaaa gttattcctc
    34681 ctaatgtctt ggcttttggt cgcccagcta aagtgatccg tcctttgaca aaagaagaaa
    34741 tacaaaaaaa caaagaaaac atccagcatt atgtcgagat aggtcggatg catgcgcaag
    34801 gaaaattcaa ggagcaaaga taggcagtag catccataaa ataaaaaagg atggtcagtt
    34861 tgagttgacc atcctttttt atttggttta gataagagag cttaaaaaag cttcctttga
    34921 aacagattaa tttaatgaag aactgttttc catgacttca tcgatcaatc catatgcttt
    34981 tgcttgttca gctgtcatat agttgtcacg atctgtatct ttttcaatca cttcaagcgg
    35041 ttgtccagtg cgttcagcta aaattttgtt taagcgttcg cgtgtttgca aaatatggcg
    35101 tgccgcaatt tcgatttcag ttgcttgccc ttgagcgccg ccaagaggtt gatggatcat
    35161 gatctcagca tttggtaaag caaatcgttt accttttgta ccagctgtca gcaagaagct
    35221 tcccattgaa gcagccatac ccataacaat tgtttggaca tctgctttga caaagttcat
    35281 tgtgtcgtaa atagccatac ctgctgtaac tgaaccacca ggagaattga tataaagata
    35341 gatatctttt tcagaatctt gagcgtctaa gaaaagcaat tgggcgatga tcgaattagc
    35401 aagatcatct gttacttggc cgcttaacat gatgatacgg tctttcaata aacgtgagta
    35461 gatatcgtaa gctctttcac ctcgtgaaga ttgttcaatg actgtaggaa ttaaattcat
    35521 aatagagttc ctccttgatt tcgtttagaa atagtgtacc ataatttagt ttctaatacc
    35581 taaaatgtag tatacatttt tggtcaaaaa aggtcaaata aaaaacttag gcaaattagt
    35641 cgcataaacc cgctttatgg tatgataagc agagaaacaa aacaaggagg aacaacagat
    35701 tgatagttgg tattactggc cttatcggag tgctgacagg tgctgtagtt gtctatatcg
    35761 gcatgatact gcaaatgcgt cacttggaaa ataaagaaca gcgtagacgt gaattggaat
    35821 tgaaagtcaa agaaatcgag acgctgaatc aattaaataa aaagatcaac gagatcttgc
    35881 aaaaacggac aacgctgatg gaagaatatg tcagctttga tgcatttgat gattgttata
    35941 tcacgattga cgattttgcg tatctccaat cattttcagc gcaaaatagt ttttatctgc
    36001 caaattatat tttagacgag ctatttaaga atatctccca tcgaaaagtg attttatctc
    36061 cagatgaaac aatgaagatt ggtggatatg cttataaagg cggacgcgtg attttagaga
    36121 attttacgga tagtctgact gaaatgatca atgaaaaaaa agctcagttg aagactatga
    36181 cgcgccagcc gattggtttc ttctcgaaag atgatgtttg gagctgatat tttgaagcat
    36241 tgccgttgca catgagagcg tggtaatgct ttcttttttt cttaatgata caatagaact
    36301 gaacggagtg aagaaagatg ttacaaatct atgcccatcg cggaagcagc ggcacccacc
    36361 cagaaaatac gcttcctgca tttgcagaag ctgtacgagt aggtgcagat ggtatcgaac
    36421 tagatgtcca tctaagcaaa gatggttatt tgatcgttat gcacgacgaa gaagtggacc
    36481 ggacgactaa cggtaaaggg ctgattcgag aaaaaacatt ggaagaactc aaaaaattga
    36541 atgcaggggg caaattcagc aaagaatttc cagcagctaa agttcctgaa ttgagagaag
    36601 tgttaggact gctcctccaa aaaaattacc gagggatgct aatgattgaa attaaaactg
    36661 atcagtatga atatcccggt atcgaagaaa aattagccga aacattaaaa agtagagatt
    36721 ggccttttca acatgcatat tgcagctttc atttggattc tttggaaaaa atcgcattac
    36781 tggaaccgga gatacagctg gacttattga tgagtacttc tgataaaaag atccaactag
    36841 cagcagaaac cccttatata gaaggagtac atccaaaaaa tacttggttg ttcgaacatc
    36901 cagtccaggc agctgatttt ccaaaagcaa ttcgtgcatg gacgattaac gatgaagaag
    36961 aaatggccaa atgtcaagaa ctcaaaatag atggaataat aactgatttt ccggagaaag
    37021 ctcttcggtg gaaacaatca acgaaaagta ggtaaaatat ggaaaaagta ttagtgatcg
    37081 tcggtccgac agcagtcggt aaaacagcgc taagtgtgga actcgcacaa ctatttggag
    37141 gagagatcat cagtggtgat tcattgcaaa tctataaaaa tctggatatc ggtacagcaa
    37201 aaatcaaaga agaagagatg aataatgttc cgcatcattt gattgatgtg atcgaaccta
    37261 ctcaacgtta ctcagccgct gattttcagc attcaggtcg acaattgatt accgagattg
    37321 cgaaccgagg gcacctacct atcatagctg gtggtacggg tctctatatc caatcccttc
    37381 tatatgatta tcagttagga gcgacagaag aagcgaatcc tgagattaga aaaaagtatg
    37441 aaacgttagc tgaaacaatt ggttcccaag cattatggaa tcagttgaaa gaaaaagacc
    37501 cggcagctgc tgagaaaatc cactggaaca atcaacgcaa agtgataaga gctctggaag
    37561 ttttagagac gacaggacat agtatcacgg cacctaaaga agagccaaag caattgtatg
    37621 attacctcat gattggatta gatacgaagc gtagtattct ttatgaacga atcaatcaaa
    37681 gagtcgattt gatgctagca gaaggtttag tagcagaagc gcgtaaggtg tatgagcttg
    37741 gagaagtcca ggccagtcaa ggtattggat ataaagaatt tttcccttat tttgaaggga
    37801 aagaaacatt ggaagaagca acagaacaag tcaaactaca ttcgagaaga tacgcgaaac
    37861 gtcagttgac gtggtttcgt aaccgtttgg atgcacagtg gtttgatctt ataagtgagc
    37921 caaatcaaaa aagtcagatt gagcaaacaa tcaaaaaatg gctggaggcg gaataaaatg
    37981 gaagaaaaag tgatcatcgt aggggtagaa accgaagcaa accagcgtta cttttcagag
    38041 tcaatggaag agctagttca gctgacgaat accgcatcag gagaagtcgt attcacaatt
    38101 acacaaaaga gaccacaagt ggatcggcaa acgattatcg gtaaagggaa attacaagaa
    38161 ttagttcaac aagcggatgc gcatgaagcg gatttgatca tctttaatca tgagctaact
    38221 ccgagacaga gccaactgat taccgatgct gttggtcttc cggtaattga tcgcgtgcag
    38281 cttatcttag atatttttgc gatgcgtgct cgttcgaaag aagggaagct gcaggttgaa
    38341 cttgctcagt tagaatacct gctgcctcgt ttagtagggc aagggaaaac attatctcgt
    38401 cttggcggtg ggatcggaac aagagggcca ggtgaaacaa agttagaaac agaccgccga
    38461 catatacgga ataaaatttt tgctgttaag aaagaattga aagaagtaga agctcatcga
    38521 gagcgcaatc gccaaaaaag aaaaaatagc gagatttttc agattggact tattggctac
    38581 acgaatgccg ggaagtcaac tatcttgaat ctattgacac aagcggatac ttattcgaaa
    38641 gatcaattgt ttgcaacgtt agatccattg actaagagat ggcgttttgc tgaaggattt
    38701 gagattactg taacggatac cgtcgggttc atccaagacc tgcctacgca actgatcgat
    38761 gctttccatt ctacattaga agaaagtcaa aatatggatc ttttgctaca tgtagtcgat
    38821 gctagttctc cagatcgtat tttacaggag cagactgtct taaaattgat ggatgaactg
    38881 aatatgaaag aaatgccgat actgactgtt tacaataagg cagaccagat cgacccagcg
    38941 atgttcacgc ctacgctatt cccaaatgta ttgatttcta ctcagacgaa agaaggtaag
    39001 atggcgttga tcgaagcaat caaacgtcag ctgatggaac taatgacccc ttatacactt
    39061 tctgtgccaa gcaacgaagg acagaaatta agcgagttga gaagacaaac aatggttttg
    39121 gacgaagcgt acatggaaga aacaaacgaa tatgaagtta aagggtttgc aaaaaccaat
    39181 tcaaaatggt taagaaaaaa ccaataaaat tttatttttt taa
//
LOCUS       DACDXO010000020        38891 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E23605_2017
            SAMN12639989-rid9881463.denovo.020, whole genome shotgun sequence.
ACCESSION   DACDXO010000020 DACDXO010000000
VERSION     DACDXO010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639989
            Sequence Read Archive: SRR10023389
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 38891)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38891)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741914.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:42:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,972
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 33 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 17 of 73
            Pseudo Genes (multiple problems)  :: 10 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38891
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639989-rid9881463.denovo.020"
                     /isolate="E23605_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(59..187)
                     /locus_tag="IWM35_001217"
     CDS             complement(59..187)
                     /locus_tag="IWM35_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HAP9919078.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
     gene            complement(190..1563)
                     /gene="rlmD"
                     /locus_tag="IWM35_001218"
     CDS             complement(190..1563)
                     /gene="rlmD"
                     /locus_tag="IWM35_001218"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HAP9919079.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
     gene            complement(1674..2729)
                     /locus_tag="IWM35_001219"
     CDS             complement(1674..2729)
                     /locus_tag="IWM35_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HAP9919080.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            complement(2739..4169)
                     /gene="gatB"
                     /locus_tag="IWM35_001220"
     CDS             complement(2739..4169)
                     /gene="gatB"
                     /locus_tag="IWM35_001220"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HAP9919081.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            complement(4166..5638)
                     /gene="gatA"
                     /locus_tag="IWM35_001221"
     CDS             complement(4166..5638)
                     /gene="gatA"
                     /locus_tag="IWM35_001221"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HAP9919082.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKATFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            complement(5638..5943)
                     /gene="gatC"
                     /locus_tag="IWM35_001222"
     CDS             complement(5638..5943)
                     /gene="gatC"
                     /locus_tag="IWM35_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HAP9919083.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            complement(6049..8085)
                     /gene="ligA"
                     /locus_tag="IWM35_001223"
     CDS             complement(6049..8085)
                     /gene="ligA"
                     /locus_tag="IWM35_001223"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HAP9919084.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTVKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            complement(8102..10336)
                     /gene="pcrA"
                     /locus_tag="IWM35_001224"
     CDS             complement(8102..10336)
                     /gene="pcrA"
                     /locus_tag="IWM35_001224"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HAP9919085.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLANPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            10491..12050
                     /locus_tag="IWM35_001225"
     CDS             10491..12050
                     /locus_tag="IWM35_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HAP9919086.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVEGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQKVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
     gene            complement(12161..14092)
                     /locus_tag="IWM35_001226"
     CDS             complement(12161..14092)
                     /locus_tag="IWM35_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAP9919087.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAAAEA"
     gene            complement(14110..15024)
                     /gene="pfkB"
                     /locus_tag="IWM35_001227"
     CDS             complement(14110..15024)
                     /gene="pfkB"
                     /locus_tag="IWM35_001227"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HAP9919088.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            complement(15021..15773)
                     /locus_tag="IWM35_001228"
     CDS             complement(15021..15773)
                     /locus_tag="IWM35_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP9919089.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLFNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            complement(15932..16141)
                     /locus_tag="IWM35_001229"
     CDS             complement(15932..16141)
                     /locus_tag="IWM35_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9919090.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHKERKPASVYMKKTRSENKKR"
     gene            complement(16152..17480)
                     /locus_tag="IWM35_001230"
     CDS             complement(16152..17480)
                     /locus_tag="IWM35_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9919091.1"
                     /translation="MIGEDKLLMTIQLTDLNEEEKKQEISDWAILTRLLIQPTFIRSF
                     TQQADPYDLRKLQEKIMLASVRDESWLVVGNDENECSFRLEDNQLLIKNVLSISVFKE
                     KQFLIRDFIQKKMIAHGVFAYMRAYSEFIYHNTKQISQRLLFEKKDEIEHLPKMKQQN
                     GEVVVDCNQFPGYDLFYQGLCFTSCWEMYYSRYYHQIIPKPIFLDIQQVEKVKELENE
                     VICIQLYRDPFNWQKPNNQMFQSYFRDQLGFDHLAWDNGVGLLKPPFVEYIYTDHTIQ
                     SVQYQNAQMQPVPKKNASFFVTKSYDIQQGDYKERRVRGTLNAQAYFPWVDENRSRMM
                     CYKVIDPTVALDNGIEAYCYYIREYLEVEVTDEKYQEYLLSLRIYVPSESLSELPLKE
                     IKHRLSDVTFKRIKKKRRSIQFDVKKGEQHLRVQFLDYRELEQLITLQKI"
     gene            complement(17845..18432)
                     /locus_tag="IWM35_001231"
     CDS             complement(17845..18432)
                     /locus_tag="IWM35_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HAP9919092.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
     gene            complement(18515..19765)
                     /gene="clpX"
                     /locus_tag="IWM35_001232"
     CDS             complement(18515..19765)
                     /gene="clpX"
                     /locus_tag="IWM35_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HAP9919093.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVLNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVERAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            complement(20012..21301)
                     /locus_tag="IWM35_001233"
     CDS             complement(20012..21301)
                     /locus_tag="IWM35_001233"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HAP9919094.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            complement(21455..22387)
                     /locus_tag="IWM35_001234"
     CDS             complement(21455..22387)
                     /locus_tag="IWM35_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9919095.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHPVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLNEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            complement(22544..23203)
                     /locus_tag="IWM35_001235"
     CDS             complement(22544..23203)
                     /locus_tag="IWM35_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HAP9919096.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSIYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAIFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            complement(23206..24231)
                     /gene="sppA"
                     /locus_tag="IWM35_001236"
     CDS             complement(23206..24231)
                     /gene="sppA"
                     /locus_tag="IWM35_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HAP9919097.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELASNVLEEGDSTQKIVKLSVDGTIAAGGSSGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAMR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            complement(24244..24534)
                     /locus_tag="IWM35_001237"
     CDS             complement(24244..24534)
                     /locus_tag="IWM35_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9919098.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAEAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            24944..26749
                     /gene="glmS"
                     /locus_tag="IWM35_001238"
     CDS             24944..26749
                     /gene="glmS"
                     /locus_tag="IWM35_001238"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HAP9919099.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDSSDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            complement(26821..28176)
                     /locus_tag="IWM35_001239"
     CDS             complement(26821..28176)
                     /locus_tag="IWM35_001239"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HAP9919100.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICAKYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
     gene            complement(28200..29369)
                     /locus_tag="IWM35_001240"
     CDS             complement(28200..29369)
                     /locus_tag="IWM35_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9919101.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            complement(29366..30253)
                     /locus_tag="IWM35_001241"
     CDS             complement(29366..30253)
                     /locus_tag="IWM35_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HAP9919102.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            complement(30442..34122)
                     /gene="nifJ"
                     /locus_tag="IWM35_001242"
     CDS             complement(30442..34122)
                     /gene="nifJ"
                     /locus_tag="IWM35_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HAP9919103.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIVPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPAMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLENANPN
                     LAKALFEKTIQDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARAERRKKMEQATKEKKEK"
     gene            34321..34842
                     /locus_tag="IWM35_001243"
     CDS             34321..34842
                     /locus_tag="IWM35_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HAP9919104.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLACPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
     gene            complement(35541..36098)
                     /locus_tag="IWM35_001244"
     CDS             complement(35541..36098)
                     /locus_tag="IWM35_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9919105.1"
                     /translation="MRNLSNRNKILIIIVVIAVFHLGTNAVLSRIILGPKPPRPEITR
                     GEFDFRLEYEVDGERIVIEDTIVALFDGFSADAGSMAWYRTWRLHLASDRRSRNILLD
                     ELEDGRRIYYVPESANYSMGDVQKKREPNPNWYPFNGVTIEYPRNKTPEIGAKFISGL
                     EDLYDRFGIRLISWEHDPPIENRFE"
     gene            complement(36277..36888)
                     /locus_tag="IWM35_001245"
     CDS             complement(36277..36888)
                     /locus_tag="IWM35_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9919106.1"
                     /translation="MNSNRGNKMEYNGSMRIVQIIFWITSGIVIIGGVFLMLPSLIFP
                     FFALISPKIPEPEITYGEFPFRIEYELDGQINIIEDTVIAEFNGCEFSAGSMKRERRW
                     RSRLASGREDLPFGDDLGIYFSRGSAQYYMGENVQSMSLKPHIALRNLEEGFKREVDF
                     ILGESGYIRTVLNFGEAQEVLTQYGITLINWEISEPIVNNFGD"
     gene            complement(37001..37258)
                     /locus_tag="IWM35_001246"
     CDS             complement(37001..37258)
                     /locus_tag="IWM35_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9919107.1"
                     /translation="MRAVHVNPTIEITMIGHSKGGAEAANSVAINTNAIIFNPATVNL
                     SAYELDSNTYTANMVAYVVEGDALNVVFGGVYTYWRINHVR"
     gene            complement(37855..38133)
                     /locus_tag="IWM35_001247"
     CDS             complement(37855..38133)
                     /locus_tag="IWM35_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9919108.1"
                     /translation="MEKHNITGSMYVDDQWFISTDEVKIAAIRRDYLQKIHKIKDPNG
                     KKARLIITLITSNGKKLKLAGSYDNVKKFEHWYLQKDSISLNKQFEKN"
     gene            complement(38167..38418)
                     /locus_tag="IWM35_001248"
     CDS             complement(38167..38418)
                     /locus_tag="IWM35_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9919109.1"
                     /translation="MKEKPINKWSLYKNSMFGVVIFGVCAGLFLFILLQTVTLFSSNP
                     TYGWRDSIALGMFAGGYGFLLLFSILTLSPIHGLIQLWK"
BASE COUNT        10632 a         8395 c         6805 g        13059 t
ORIGIN      
        1 taaaaagact aagaaacaat aacaaaagtt aacacttctt agtctttctt tagcacgtct
       61 atttattaaa gattttaggg ccttttctca tgtttttacg atcattctta ggtgtttcca
      121 tttgtttgga tcggcctcca ccttgtttct ttttgatcaa ttctttcatt ttttcttttt
      181 ggtccatttt tattctcctt taacgaatga agcgactaat tcgatatgat gggtttgcgg
      241 gaaaagatct acaggctgaa ctttcttcac ggcataacct gcttctgcaa ataacttagc
      301 atctcttgca aaagttgccg gattacatga gatatagacg attcgttctg gtgacatttc
      361 gattgctgat tcaataaaat gatgatctaa tccttttcgt ggagggtcaa ctacgatcac
      421 agatggtttg atcccttttt cttgccattt tttcataact ttttctgctt ttccgacttc
      481 ataatgaaca ttttcgattt tattatttaa agcattgaac tgtgcatctt caattgcttc
      541 tggaataact tccatcccat acaccttatc cactctgtct gctaaagaca agccaatcgt
      601 acctattcct gaataagcgt cgatcacaat gtctttttta tctaactcgg caaactcgat
      661 ggctgtttgg tacagatact ctgcttgttg agtatttact tgataaaatg actttgctga
      721 gattcgatag gtttttccta atagctgatc ttcgatatag tctctgccat ataaaacttt
      781 ttctttgtct cctaaaatca catttgtttt ttcttcattg atattttgga tgaccgaaac
      841 cacttctggc aattcttcat gaatagcttc agcaatttcc ttggatttaa agaatttttc
      901 tttacgagta actaaaacga ccatcatttc atgggaataa tgaccacgac ggataatgat
      961 gtgacgaagc tgtccttcat ttttttcttc attatatcct cgtacttgga accgttgcaa
     1021 gatatctctg actactttga ttgcttgatc gatgacagga tcttggatat aaaaatcttc
     1081 gatagggact agatcatggc tattcttttt gtaaaaaccg gtggctaact gtccatcgat
     1141 acggcgaaca gggatctgtg ctttattacg gtagccgaat ggttctgcca ttccgaccgt
     1201 tggaagtaca ggaacttctg gcatcttagc aatcttcgcc attacatgct ctacttgttt
     1261 ttgtttgaag acaagctgtt gttcatactt caaatgggct aaaggtgcga tacctatgcg
     1321 aagtaatgca ttgttttcta caggctgacg atcaggtgat tgtgtaataa atgtttcgac
     1381 ttttccatat ccaaacttat tgcctgcttt tactactagt atttctactt cttctccagg
     1441 gagtgcattt tcgataaata atggataacc atcgattttt gctaccccca ttccttcata
     1501 actcaagtcg acgatttcga ctgtatatcg ttcgtttttc tttactattt gctgttctgt
     1561 catattttgc tcctttaagc ctatttttca atcctactga actctactat aaatcagcta
     1621 attacacaac taaaaccggc actgacctta ctacgttcag caccggcttt tgattaattc
     1681 ttttgattag gtgtttcgtc tgctattttt ccgtcaccgt cgatatcttc atccgttaaa
     1741 cgttcgactt cttttacgaa ttctttgctg acaacctcta attcttcttc atctgtacct
     1801 gtgattgctt catcaggaat ctgatcggta ttggcaaaga actcaatgtg ttgatgaaga
     1861 ttttggaaat gcatcggtgc ttccccgccg tattcaccat ctaagttgat cattactggt
     1921 cgattgatat catcttctgt ttcaacgaac aaactgcttg ttttggtata gattattctt
     1981 ggatcatcga catgtcgacc gccattcagc atgagtgctg caatatgcaa gatctcaaag
     2041 atatttgctg tcttcacaat gattaaagaa aacttcccat catccaattt ggcatctggc
     2101 gcgatctgtt cgaatcctcc aacagagttc gtcagcccta ggaaaaacat cgaagcattt
     2161 ccgatatatt ccccttcatc atacttcaag cgcattttga ttggctttac tcgagggagc
     2221 atctctgctc ctttcgcaag ataagcaaga taaccaaaaa tactctttag ttctgatgga
     2281 acttcataag tcaattctgt caaatagcca cctgcagcga tattgatgaa gtaactgtta
     2341 tcagaaaggc cgatgtccat ttttacggtt tggtgttttt ttatcacttc tgctgctgct
     2401 ttaatattat ctctaggaat ttttaatgct cgagcatagt cgttcgttgt accagctggg
     2461 atgatggcca ttttaggacg gtgttcaagc ggagcgattc cattaaccac ttcgttgatt
     2521 gtgccatcgc caccagctgc cactactaga tcaaaaccta gttctgcgac tctacatgct
     2581 tcattcttgg ctgaatctgg ttctggtgta gttgcatagg cgctggcttc aaatcctgct
     2641 tcttctaatg cctgtaggat atccgccaaa ttttttttca ataattcttt tcccgaggtg
     2701 gggttataaa ttacgcgtgc ttttttcatt ttcagcctct atcgtttcgc taattcatct
     2761 ttaagtagct tattcacgac accaggattt gcttgtcctt tagttgcttt catgatttga
     2821 ccgactagga agccaaccgc acgatctttt ccatttttga aatcatcgac agattgttgg
     2881 ttgttatcca aaatttcatt aatgattggc agtagtttgt tcggatcaga taattgcacc
     2941 caaccatttt tttcaactac ttcttgggct tctccgccat ttgtgatcaa ttcacggaag
     3001 acttttttgg cgatttttga gctgatcgtt ccatcggcga tcaaacgaat cattcctgct
     3061 aagttctcag gtgtcaattt tgtctcagct aattccagtt tctcactgtt caagtaagca
     3121 gaaacttcac ccatcaacca gttagatgct tgcttggcat ctgcaccatt ttccaaaaca
     3181 gcttcaaaaa aatcagacat ttcttttgta agtgtcaata ccattgcgtc atactctgga
     3241 agtccaaact cacggatata gcgttcgcga cgagaagctg gcatctcagg caaactagct
     3301 tttacttttt gaatccattc gtcatcgatc acgaatttcg ggatatcagg ctctgggaag
     3361 taacgataat cactagaccc ttctttgaca cgcatcaaaa ctgttttatt cgttgtttcg
     3421 tcaaaacggc gtgtttcttg ttggatttct cctcctgaaa gaagaacttt tgcttgacgt
     3481 ttttcttcaa aagctaaacc ttttttcacg aaagataaag agttcaagtt tttcaattct
     3541 gctttcgtac cgaactcttc ttgtccataa gggcgaagag aaatattggc atcacagcgc
     3601 atagagcctt cttccatttt cacatcgctg acttctgtaa attggataat tgatcgcaat
     3661 gcttctagat aagcgtatgc ttcttctggt gaacgcatat cagcttcaga aacgatttcg
     3721 atcaaaggcg tgccttgtcg gttcaaatca acgtaagaat aaccgccaat accatgcatg
     3781 ttttttccag catcttcttc taaatggaca cgttcgatcc ggattttttt ctttttacct
     3841 tctacttcga tttcgatcca tccgtcatga ccgattggtt gatcaaattg ggaaatttga
     3901 tatgcttttg gattatctgg gtaaaagtaa tttttccggt caaaatgagt atctttagag
     3961 atttcacagt ttaatgccaa agctgctttc atcccgaatt ccagcgcagc tttattcatt
     4021 actggcagca cgcctggata accccagtcg atcacgtttg tattgctgtt tggttctgca
     4081 ccaaaatgag ctggcgctgg agagaaaatt tttgagttgg tttttaactc gacgtggact
     4141 tctagtccaa tgactgtttc aaaattcatt cgtctttccc tcctaaaata gctggttgtt
     4201 gtttatggaa ttctgttgct tgttcaaacg catatgccgc ttgatacatc gttttttcat
     4261 caaaataatt accgatgatc tgcaatccaa ctggtaatcc ttctgataaa cctgccggta
     4321 cagacatacc tggaagtcca gccaagttga cagggatcgt taagatgtca ctcatgtaca
     4381 ttgtgattgg atcgttaatg ttctcaccga ttccgaatgc aactgttggt gaagatggcc
     4441 cgatgatcaa gtcataatct gcaaagactt tttcaaaatc tcgtttgatc aatgtacgga
     4501 cttgtcccgc ttttttgaag taagcgtcat aataaccagc acttaaggag aatgtaccta
     4561 acatgatacg gcgttttact tcttcaccga atccttctga acgtgagttg acatatacat
     4621 catctaatga tttcacattt tctgagcgat aaccgtagcg gataccatcg aatcgttgca
     4681 agttggaact tgcttctgat gaggcaatga tataataaac agccacaccg tattttgaat
     4741 gaggcaagct gacttcttcc acaacagcac ctaatgaacg gaatgtttct gctgctttca
     4801 atacagcttc ttttacgcca ggggccacac cttcgcctaa gtattcttta ggtaaagcaa
     4861 tcttcattcc tttgatgtcg cctgtcagtc cctctgcaaa atcaggaact gaaacgcctg
     4921 aagaagtacc gtctttttcg tcataaccgc tgatagctgt caatgctaat gcattatctt
     4981 tcaccgtacg ggtcattggt ccgatttggt caagagatga agcaaaggca atcaaaccaa
     5041 aacgtgagac acggccataa gtaggtttca tccctacgat gccgttaaaa gctgctggtt
     5101 ggcggatact tcccccagta tcactaccta aagaaactgg aatttgtcca gctgcaactg
     5161 ctgctgcaga accaccagaa gagccaccag gtacttttgt tgggtcccaa gcattttttg
     5221 ttttcttgaa ataagaagtt tctgtacttc cacccatagc aaattcatcc atgtttagct
     5281 taccaacagg gatcatatct gcttgataga ttttatccat gaccgttgca tcataaattg
     5341 gttcaaaatt gtaaaggatc ttagatgcag cggttgtcaa gatatctttt gttacgatat
     5401 tatccttgat accaattgga ataccagcaa ggacgttgct ttcatcaatc cctttttcat
     5461 ctaaagcttt tgcctgttcc aatgcttttt cttcgttcaa tgtaatgaat gcttcgacct
     5521 caggctctgt ttctttgatt cgttcaaatg ttgcttttgt caaatcttgt acagtgattt
     5581 ctttcgatac aagcatatga tgcagttctt ctagagaacg attgtgtaat tctgtcatta
     5641 tgcgccagcc tccccattat cgatgattgc gggtactttg ataaagccgt cttctgtttc
     5701 cggcacattt ttcaataatt catcgcggtt ccatccagct tgcgcccgat cttgtcgcat
     5761 cacgtttact gtttcaacca cattcgacgt aaatggaaca ccttctgtat ctacttcttc
     5821 cagcatttca accatatcga tgattttacc taattggtcg gtaaaacttg caagttcttc
     5881 atctgcgaac gcaagttttg ctaatttggc aacgtgtttc acttgttctt cactaatcgc
     5941 catactaccc cttctttcta aacgagttaa aaactttgct ctctatttta acacaagagt
     6001 gccgtgtctg aaattgttta ttgaaatttg tctgaaaaaa ctcatttttc attcttctac
     6061 tgagcgactt ttagccaatg catctgccat ttgatcttct gtccaaactt cgattcctaa
     6121 ttcttgtgct ttggtcagct tgctgccagc atcttctcct gctaccacga tatccgtttt
     6181 tttcgatacg ctaccagtca ctttcccgcc aagattttcg atcgtctctt ttgcttcttc
     6241 acgagtaaat cgtgttagtt taccagtaag gactactgtt ttttctttaa aaggagactc
     6301 gacttcttgc aattgggcat ttcgaagccc tttgtattcg aaattgacgc ctgcttgttt
     6361 taattcattc atcaattcat ggacttcttc gttactgaaa taagtgacta cactgtcggc
     6421 aatgatatca ccgatcgtat caagcgcaat aatcgactca tagtcacttt tactcaatgt
     6481 ttctagatca ccgaaatgtt ctgctaaaat ttttgctgct ttcgctccga catgtcggat
     6541 tcctaacccg aaaatcaacc gctcgaccga attatctttg ctgttgtcga ttgctgtcaa
     6601 aatattattt gctgattttt cttttatttt gtccaacgtc aataactctt cttctgtcaa
     6661 tttgtaaaga tcagcaacat ctgctactag ttttttatca tacatctgtt ctagtacgcg
     6721 cggccccaac ccatcgatat tcatcgcatt tcttgaaaca aagtggttca aaccttcttt
     6781 catttgagca gggcatttag gattgatgca ccgtaaggct acttcttcat ccaaatggac
     6841 aagttcactt ccacaaactg gacaatgtgt aggcaattgg tattcttcgc tgtctttagg
     6901 tcgtttatct aacacgactt gcgaaacttc tgggatgatg tctcccgctt tataaatcag
     6961 aactgtatct ttcaagcgga tatccttcaa cttaatataa tcaccgttat gcaggctggc
     7021 acggcttact gtcgttcctg ctactcggac tggtgtcatg atagctgttg gcgttactac
     7081 ccctgttcga ccgactgtcc attcgatgtt ttcgattagc gtttcaactt cttctggcgg
     7141 aaacttgtaa gctgctgccc atctcggtgc tttgacagta aaaccaagtt gatcttgtag
     7201 cgagaattca ttcactttga tgacgattcc atcgatttca tagggcaaat ccacacgttt
     7261 gtcatgatat tcttcaatgt atgaccacac ttcatcaatc gaatgacata gacgtttttc
     7321 ccgattcgtg tggaatccaa ttttttctag ctcttctagc gcatcatact gggtttttgc
     7381 ttgcatagga ccgaaatcag ctactgtgta taaaaaagtg tccagatttc ttttcgctgt
     7441 gattttagaa tctaactgac gcaaagaacc agctgcggcg ttccgaggat tcgcaaagat
     7501 atcttttccc tctgcttctc tgtcttggtt caattggaca aatgaccgtt ttggcatgaa
     7561 acattcgcct cttacttcga tgttcattgg ttcttttaat ttgattggga ccgaccggac
     7621 agtttttagg ttttcagtga tgttttctcc tacagttcca tccccgcgag tcgcacctct
     7681 cacgaaattt ccatcttcat aacgcaaaga aacggaaagt ccatctattt ttagttcaca
     7741 gcaatagtca actacacgtc ccactgcttt ttgtacacgt tgatcaaaag ctatcagctc
     7801 ttctttgctg aaaacgtcgt tcaaactgta aagcggaata tcatgagtga ctttttcaaa
     7861 accttctaat acttttccgc ctactcgttg agtgggagaa tcagacgtga tcaaatcagg
     7921 atattgttcc tcaattgcta ctaattctgc gtattcttta tcataaacat agtcttccac
     7981 tgttggttta tcttctacgt agtattcgcg agaccattgg ttcagtcttg tacgaagttc
     8041 ttctgctcgt ttagcagctt cgtcaatcgt catttcatgt tccatcattt accctccact
     8101 actaagcttt ttcaattggc gcaaaggcag ccaaaagacg ttttactcct tgacttggga
     8161 aagccacatc tagttctaga tccttcgacg tcccgcttac tcggacgact gttcctagac
     8221 cccatttttt gtgtttgact ttatctccag gtttccagct gttattttcc cctccagtag
     8281 cacttttaga gggaacaacc gctgtctctg gctgtttata gctatatgct gtttttttag
     8341 cgaatacagg tgttttttta ggcgtaggac gcatgccttc gatttccagt aactcctgat
     8401 cgatttcttc tacaaaacga gaaggtcggt tatattgcgt ccgtccatat aatgttctgg
     8461 agaaagcatt tgttaaatac aacgcttctt ctgctcgcgt gatccccaca tatgcgagac
     8521 ggcgttcttc ttccagttcg ctttcttcca tcaatgctcg agataaagga aaaaccccct
     8581 cttctagacc aatcaggaat acaactggga attctaatcc tttggctgca tgcaaagtca
     8641 tcaatgtcac ttgggaagca tcttcttcta aattgtcaat atcggaaaca agtgccagat
     8701 catttaagaa gaccgttagt ttttcttctg gtgcatcagc gtcttcttca ttttgctgtt
     8761 cgaattgttt gtcgaattct tgtgtgaccg ttaaaaattc ctccaagttt tcgatgcgtg
     8821 cttgtgcttc taatgtattt tggattttta agtcttctag atatccgcta cgatccaaaa
     8881 cctctttcgt caattctgtc actgtcaagt atgggatcat ctgagtgact tcttggatca
     8941 tctctccaaa tgcacctaat tgctgtcctg cttttccacc gatatttgcc agatccacat
     9001 tttgcgctgc ttcaagcagt ggccattcat gaagagaagc aaaactgcgt agtttttcga
     9061 tagatcctgg tccgatgcct cgtttgggcg aattaacgat acgctcaaaa ctgattgaat
     9121 cttgaggatt tgccagcaca ttcaaatacg ctaaaatatc tttgatttct ttacgatcgt
     9181 agaacttgtg ccctccaacc attttataag gaatatttgc tttcaacaga gtttcttcca
     9241 ttacacgaga ttgtgcgttc gtccggtaca agatggcaaa gtcgccgtaa ttccgatgat
     9301 tgcttcgaat ctcttcttgc atccgatcta cgataaaacg ggtttcgtct cgttcattat
     9361 ctgcacggta gtaagtgatc ttgtttcctt ctttgttttc tgtccacaag ttttttggtt
     9421 ttcgattgct attgttttca atgacttggt ttgctgcgct aaggatgttt ttcgtggagc
     9481 gatagttttg ttctagcaaa attactgcag catctggata atctttttca aaatccaaga
     9541 tgttctgcat atctgcaccg cgccagccgt aaatactttg atctgcatct cccacgacgc
     9601 acaggttcct aaatctacct gccaataaat tgactagcgt atactgtgcg tgatttgtat
     9661 cttggtactc atctacatga atataatgga atttgttttg ataatatgtg agtgaatcag
     9721 gatgttcttc aaataatcga atcgtattca tgatcaggtc gtcaaagtcc atacattgat
     9781 tatattgaag ctctttttgg taagcagcat aacattttgc cgcaatctct tcgtaataac
     9841 tgccttgcat ttttgcaaaa tcctgagggg tctgcaggct gttttttgct tgagagattg
     9901 ttcctaatat acttcgagga tcatattttt tcggatcaat gttcagttct tttagtatcc
     9961 gtttcatcaa ggtcagctgt tcagaagaat cgatgattgt aaagttccgg ttatacccga
    10021 taaaatcaac atctcttctt aaaatacgca cacacatcga gtgaaaagtc gatacccaga
    10081 catcttcgcc tccagatgcg agaatcgcat tgacacgttc tttcatttct tttgcagcct
    10141 tattggtaaa agtaatcgct aggatgttcc aaggattgac ttctttttcc tcaatcaaat
    10201 aggcaatgcg gtgtgtcagc actctcgtct ttccacttcc tgcacctgcc attagcagta
    10261 atggtccgtc agtgtgaaga acagcttctt tctgacgggg gttcatcccg tttaataatt
    10321 cagctttgga tgacattaac aacatccttt cttttatcaa tcttgttaat tatagcattt
    10381 tcttggcttt cattaaacta gctagttcaa aatcatttgt ttctttttat ggatttttcc
    10441 ttgtcaattc gttgatttcg cgctaaatta aaagaaagcg aggcgtttga atggaaaaaa
    10501 aaggaaaaat caagaaatta gataagactc agatatattt tattctaaaa ggaatatttg
    10561 ttggactgct gacaggaatc gtcgttagcc tgtttcgatt atctatcgaa aagctaagcg
    10621 atttagttcg aagtatctat gaaatgagtc gggaacagcc gatttactta attggcctag
    10681 ctatttgctg catccttgca gcatttttcg tgggttatct ggtcaaaaat gaaccggata
    10741 tcaagggaag cgggattcca caagtagagg gacagcttcg cggggaatta tcaatgaact
    10801 ggttctctgt tttatggaaa aagttcattg gcggtgtact ttcagttggt gcgggccttt
    10861 ttctaggtag agaaggacct tctatacagc ttggcgcatc cattggacaa ggagctggac
    10921 aattgtttcg gagtccatct tcagaagaaa agattttaat ttctagcggt gcaagtagcg
    10981 gacttgcagc agcattcaat gctcctatcg caggattact ctttgtttta gaagaaatcc
    11041 atcactcctt ttctccactt gtttggctga catcatttgc ttcagcaatc actgctaact
    11101 tcgtctctct ttactttttc ggcttgagac ctgttttata tctaggtgac cttccttctt
    11161 tgccgttgaa atattatggt tccttagtga tcttaggagt acttctaggc atcttaggtt
    11221 ttatttatca gaaggtcttg ctggctttgc caaaatttta caaccgattg cctttaccag
    11281 cttattttca cgggttcgtt cctttcttgc tgattttgcc aattggttat ttatggccta
    11341 gtactctagg cggaggcaat caaatcgttt tagcttttgg acatgcttcc ttgccaatca
    11401 ttatgattat cggtttgttt gttcttcgat tcgttttttc catgatttct tacggtggtg
    11461 gtcttccagg ggggattttc cttccgatat tgacattggg ggctttaatc ggtagctttt
    11521 atggaaacat tatggttgat ttcgggatgg atccaatcta catcaaggat tttgtcgtta
    11581 ttgccatggc tggttatttc acagcgattg gaaaagcacc gctgacagca attattttag
    11641 tgacagaaat ggttggctct cttaatcatc tgatgccttt aggattggct gctcttgttg
    11701 cttatatcgt caatgacctt ctcggcggta atccaattta cgaatcatta ttggaacgtc
    11761 tacttggaaa tactcagacc gcctcactaa aaggtgcaag gacaactttt gattttccag
    11821 taacggcaga gagttcttta gacggattaa tggtacgaga cttcaattgg cctaaaaata
    11881 tgctgctaat ctctatacgc agaggaaatc aggaaatctt gactcacggt gatacgatca
    11941 tgaatgtagg agatattttg gttatcttga ctgatgaagc aaacttgcca agaatcaagc
    12001 aggaaatcga ggctaaatct tctatcacaa atttgaagca atcattttaa cgaacatgga
    12061 aaaactattt tacaacaact attcatctac actacttgat tctatatgat tctataaaaa
    12121 aagaagagac tgggaaaaac cagcctcttc ttttttattt ctatgcttct gctgctgcag
    12181 gagcttttct caataaacca taaatcacac cagatacgac tgctccgata gcaataaaca
    12241 ccaaatacat gaccgggtga ctcagtaaga atacaacgaa aatcccgcca tgaggagcca
    12301 gtaatttgat cccgaaagct ccgactaatc cgccagttaa agcagaacca ataataaaac
    12361 ttggaatggc acgaatcggg tcagctgctg caaatggaat cgatccttcc gtgacaaacg
    12421 atagtcccat cacgatgttc gtcaaaccag catcttgatc tgttttgctg aatttattct
    12481 taaacaaacg tgtagcaacg aaaattgcca atggaggtac cattcctcca gccataacag
    12541 aagccatcac gacacttccg ccagttgcaa cagtcgtagc taaagttgct gtaccaaaca
    12601 cataagcggc tttgttgatt ggaccgccta aatctgccgc catcattcca gctaataatg
    12661 caccaagtaa agcagcattc gaaccactca tacttgtcaa gaaaccgttc aatgcatcat
    12721 tgattgcttt catagggaca ttgaccagca acatcaagaa accagtaatc agtaagccaa
    12781 acactggata aaagaggatc gttttaattc catccaatga ttttggtaag ttcttgaata
    12841 atttacgtaa aaatacgatg acataaccag ctaagaatcc accaatcagt gcacctaaga
    12901 atcctgcacc gcctgtatta gctaatgcac cagcagcaaa tccaacgatc aaaccagggc
    12961 ggtcaccgat acttgaagca ataaatcctg ctaataccgg cagcatgaaa ccaaatgcag
    13021 cttgtccgat ttggttgaac cagctagcag cttgattgta attccctaga tttgataatt
    13081 gatcatgtgg cacgccaata aattggtcga tcatgaaaga aagcgcaata gcgattcctc
    13141 caccaataac gaaaggtaac atgtgtgata cgccgttcat caaatctttg taaattcttt
    13201 gtccgatagt accatccgca tttccttctt ctgaactgct ttcttttcca tcaccatgga
    13261 atactggtgc agtaccacta agggctagat tgatcagctc ttctggttta cggataccat
    13321 cactgacagg acgattgatc agttcttttc cgtcaaagcg attcatctct acttttttgt
    13381 ctgctgcaac gattacacct tctgcacggg cgatgtcttc agcagtcaat cggtttttga
    13441 ttccttctga accgttcgtt tcgactttga tctccacacc catttctttc gcttttttct
    13501 tcagagcatc ttctgccatg taagtgtgag cgatccctgt tggacaagct gtaacggcaa
    13561 caacgaattt tctttctgga tgagcagaag cttctgcagc ttgtgtttct tgttctgctt
    13621 tttcttctgc ttctttttgc tcttcagctg tttggaacaa gtcttggact tcttctggtg
    13681 tatttgcttc ttttaatttt cctacgaaat ctgcatcaat cagcaatcga gatagtgctg
    13741 ctaatgcttg caaatgtgtg tcgtttgcgc cttctggagc tgcgatcatg aaaaacagat
    13801 aagttggctg tccgtctaag gcttcataat ctactccttt ttggcttttt gcgaataaaa
    13861 ctgttgcttc tttaactgca ctgtttttcg cgtgaggcat ggcgattcca tcaccaagac
    13921 ctgtagaagt ttgtgcttcg cgggctagaa ttccttcttt atatgtttcg atatcggaaa
    13981 tccggccggc atcaaacatt ttttggacca tttcatcgat tgcgcctttt ttatcagtcg
    14041 cttgcaaatc catgatcatc gcttctttga ccattaaatc cttgatattc atgattatcc
    14101 tccttgaaac taatcaattt ttgtgatttc aacttctggc agcaactcat caatcaattc
    14161 gcgagctgcc aagtcatcag aaaaagctgt tgcgcttccg caagcaactc cccatttaaa
    14221 ggcttcgatt ggatctttcg tacggctgta agccccaata aagccggcaa tcattgaatc
    14281 ccctgcacct actgaatttt tcaatgggcg aactaataca ttcgttttat aagttccttg
    14341 ttccgtgaag agcaaagcac cgtctccagc cattgagatc agcacattct gtgccccttc
    14401 agctagcaat tttttaccat acggaatgat ttcttctaca gattggaagg ttgtattgta
    14461 aagctcagct aattcgtgat tgtttggttt gatcaacaat ggtttcttaa caagtgcatt
    14521 ttttaggtca tcacctgttg tatcaatcac gaaatctgca cctttttctc gaatgatatc
    14581 aatcagttct tgataaaatc cttttcgcag gctagccggc gcgcttcctg acaatacgac
    14641 gatatctcct tgtcctactg ttcctaatgc ttgcttcaac tcatcgattt cttcatctga
    14701 aatagatggg cccaaaccgt taatttcagt ttcagtatct gattttagtt tgatattgat
    14761 tcttgtatct tcttttacgg ttgtgaacaa agactggatt tgttcttttt caagccactt
    14821 cgaaataaat tcgccagtga accctcctaa aaaccctagt gctgtagatt gagaatcgat
    14881 ccgtttcaaa atacgggaaa cattgatccc ttttcctcca ggtaacttga agtcttcctt
    14941 cattcggttc aaatcgccta gttttaaccc atctacgcgt acgatataat caattgatgg
    15001 attaagtgtt actgtgtaaa tcattgtatt gcctccttaa ttgttgtctt ttcacggaat
    15061 tcattaacga attctaatgg acaacgctcg gttatgatcg tggctgcttc caaaggtgca
    15121 acagaggtga aggacaactg gttgaatttg gtatgatcaa tcaatacaaa tgtctcttcg
    15181 gaatttttca tggctaaacg tttaacagct gcctcttctg ggtccggagt cgtgaatcct
    15241 tgttcaagat gggcaccgtt cattcccata aatgctttat taaaatgaaa attttttagt
    15301 tgttctatcg cattagatcc taaaacagca ttagttgcca gctttatcgt tccacctaaa
    15361 ataatggtag caagctccaa atcaactaaa tttgccgcat gttgaacgga atttgtcact
    15421 actgtgatct gttttccttg cagaaaagga atcatttcta acgtagtaga gccagcgtct
    15481 aaataaatga catcttcgtc attaatggtt tcagctgccg atttggcgat ttgctgtttt
    15541 tcgtgaacgt ttttgagtga tttttcactc atattctgct caaaattcaa taattgctct
    15601 ttcttagctc cgccatgaac tctttctaga aacccactat tctccagctc ttgtaaatca
    15661 cgtcttatcg ttgattcaga agcattaaaa aggtttacaa gatcttgtga tttcacgatt
    15721 ttctgctgtt caagcaagtc taggattttt tgtctacgtt cctctgtaag cattttcacg
    15781 atcacctcaa tacaaataat agcatatttt ttttctactc gcaatataaa tcagtcaaaa
    15841 acgttcataa aaattataaa tcagtcaaaa aaaaataaaa aaccagtcaa aaagactggt
    15901 tttttatttt tttgtctatc atcaagccgt tttatcgttt tttattttcg cttcttgttt
    15961 ttttcatata aacagaagca ggttttcttt ccttatgaag aagataataa taaagtccca
    16021 tcccgccaaa catgaccaaa aaaccgatga ctgatcgaat aggaaaatta ggaacaaaaa
    16081 agagacagac ggataataat gatgccggaa ccattattcg ataatagagt tgtttatcca
    16141 ttttcttcac ctcatatttt ttgtaaagta atcaattgtt ctaattctcg gtaatctaaa
    16201 aattgcacac gcagatgctg ctcacctttc ttgacatcga attggatact tcgccgcttt
    16261 ttctttattc gtttgaaggt aacatcagag agtctatgtt tgatttcctt taacggtaat
    16321 tctgacaggg attctgaagg aacatagatt cttaaggata aaagatactc ttgatatttt
    16381 tcatctgtta cctctacttc tagatattcc ctaatataat aacaataagc ttcgattcca
    16441 ttatccaaag caacagtcgg atcgataact ttatagcaca tcatgcgcga gcgattctcg
    16501 tcaacccaag gaaaatacgc ctgagcattt agtgttccgc gaacacgcct ttctttataa
    16561 tctccttgtt gaatatcata actttttgtt acaaaaaatg aagcattctt tttaggaaca
    16621 ggttgcattt gtgcattctg atactggaca gattggatcg tatggtcagt gtaaatgtat
    16681 tcaacgaatg gcggtttcaa taaaccgaca ccattgtccc aagcaagatg atcaaaccca
    16741 agctggtccc taaaatagga ttgaaacatt tgattgtttg gcttttgcca gttaaagggg
    16801 tctcgataaa gctggataca tatgacttcg ttttctaatt ccttcacttt ttctacttgt
    16861 tggatgtcga gaaaaatcgg tttagggatg atttgatgat aatagcgact ataatacatt
    16921 tcccaacacg aagtgaaaca taaaccttgg taaaaaagat cgtatcctgg aaattggttg
    16981 caatcgacta ctacttcccc gttttgttgt ttcattttag gtaaatgttc gatttcatcc
    17041 tttttctcaa acaatagccg ttgagagatc tgttttgtat tgtgatagat aaactcgcta
    17101 taagctcgca tataggcgaa caccccatga gcaatcattt ttttctgaat gaagtctcga
    17161 atcaaaaatt gcttttcttt gaaaacagag atagacaata cattttttat caataattga
    17221 ttatcttcca agcgaaaact acattcgttc tcgtcatttc ctacaactaa ccaactttcg
    17281 tctcttaccg atgctagcat tattttttct tgaagctttc gaagatcata cggatccgct
    17341 tgttgtgtga aagagcggat aaatgttggc tggatcaaca gacgtgtcaa aatagcccaa
    17401 tcgcttattt cctgtttttt ttcttcttca tttaagtctg tgagctggat agtcatcaat
    17461 aatttatctt cgccaatcat tttctcacct ctttaaaatc tttttataat ttaattataa
    17521 ccaacgataa agaaaaacta aaactttatg tcttagaaac caatttggac gattacatga
    17581 tagactaagt aaaaagaaac aaagaggtgt ttacaatggg gttgaaattt gaacgtacag
    17641 ataatttaga taaacttttc gaatcatttg ctgtcgatcc ggaaaaaaca gttgcaccaa
    17701 aagaagagat cgatcgattt ctaaaaccac agccacaaaa tgaaaagaaa aaagacaaga
    17761 aacaaaagta agttaagctc aaaaaaagac ttaccagcca aacttttatg aaagagtttg
    17821 actggtaagt cttttttatt ctttttatga ttcaattgct tgttcgattg ctgcccacgc
    17881 ttcttctttt ccctgttttg taacggaaga aaagagaata aacgtgtcat tgcgatcaaa
    17941 atccaacgct tttttgattt cgctctcatg cttgttccat ttaccacgag ggatcttatc
    18001 tgccttagtg gcaacaacaa ttacagggat atcataatac ttcaaaaatt gatacatctg
    18061 gatatcttct tgagaaggct tatgacgcaa atctactaaa gaaacgaccg cacgtagctg
    18121 gtctctagtc gttaaatatg tctcgatcat ctttccccat tttgctcgct cagttttgga
    18181 tacttttgca tacccatatc ccggaacatc gacaaaatgt aatgcgttct cgatcaaata
    18241 aaaattcaat gtctgggtct ttcctggctt accggaagta cgggctaaat tctttcgatc
    18301 aatcaatgtg ttgataaaag aagatttccc tacatttgat cgtcctgcca gagctatttc
    18361 tggcaattgt gtatctggat attgttttgg tgccactgca cttatgataa tttcagcttg
    18421 atggactttc atggtctctc tccttaacaa ggtgatgaat aaagcaagcg gctgtcgcaa
    18481 aagccaaaat gacttttacg gcagcctctt ttcatcaacc cgcttttttc tctgaatctt
    18541 tatagattac tgttggctca ccagacaatt cagctgcttc ttttgtgata atcacttttt
    18601 cgatgttttc atttgaaggg atatcaaaca tcacatccat catgatttct tcaatgatgg
    18661 aacgaagtcc tcgagcaccg gtattgcgtt cgattgcttt tttcgcaatg gctctcaacg
    18721 cttcaggttc gaattcaagt tcggtattat ctagagaaag aagtttttga tattgtttga
    18781 ctagcgcatt tttcggctca gtcaagatcc gtaccagatc atctgttgtc aatttctcta
    18841 aagctgccat aacaggtaat cgtccgataa actcagggat caatccgaat tttagtaaat
    18901 cttcaggaat gatatgctgc atgacacttt cgtcttcaga aagtttttgg ttttgtgtac
    18961 caaatccaat cgtcttttcg cccatacgat tcttcacaat cgtttcgatg ccatcaaatg
    19021 caccacctac gatgaacaat atgttcgtcg tatcaatctg aataaattct tgatgtggat
    19081 gtttacgtcc tccctgaggt ggcacgcttg caacagttcc ttctagaatt ttcagcaagg
    19141 cttgttggac accttcgccg gaaacgtcac gagtaataga tacattttcg cttttacgcg
    19201 caattttatc gatctcatca atatagatga tccctttttc tgcacgttcc acattgtaat
    19261 cagcagattg aagcaattta agcaaaatgt tttctacatc ttcccccaca taaccagctt
    19321 ctgtcaaact tgtcgcatct gcgattgcaa aaggtacatt caatgttttt gctaatgttt
    19381 gtgctaaaaa tgttttacca gaccctgtag gtccgatcaa gcagatattg cttttttgca
    19441 gttcaacatc atctgtgttt tcttgtgctt gctgattgac acgtttatag tgattgtata
    19501 cggcaacagc taacgagcgt tttgcgcgat cttgtccaat cacgtagtta ttcaatactt
    19561 caagaatttc tagtggttta gggacttcag tgaattcgcg tacagcttct tcataaaatt
    19621 cttcatcaat gatttcttta cataggtcga tacattcatt acagatgtag acccctggac
    19681 cagctacgat ctttttcact tcttcttggg ttttcccgca aaaagaacag cgtacggtct
    19741 cattgctact tggattgtca tacatggtct tcacccctta acttgcaaaa agtatgcaag
    19801 aaactatcaa agactttaca gtcttttact atcataacat agtttagtaa aaagaaaaag
    19861 tgtttaacaa aggatcaact gacctatacc tttgcatttt ttactcttaa caaaaaataa
    19921 ggaactgtga caaaatcgtg tcacagtccc ttatttcgaa taaacaattt tctagaagta
    19981 gcttcttcag aaatgattta ttacttgatt tctattcttt cgcttctgtc gctgtacctg
    20041 tgatcaattc gattgctttt ttcattgtca catcatgttc cagcatatct tctgtcaaca
    20101 cgcgtttgat ttgttcgatt ggcatgttgt atgtttctga taattcttgg atttctgcat
    20161 tgatttcttc ttctgttgct tgaagatttt cagcagctgc aattgcttcg atcacaagat
    20221 ttgttcttgt gcgcatttct gcttcacctt cgaattgttt atgcaagtct tcttctgtag
    20281 aaccagttaa ttgatagtac atttcaggag agataccttg acgttgcatg ttgttcaaga
    20341 attcgtccat tgaacgatgt acttcatcat gaaccatcac gtgtggcaat tcaacgattt
    20401 ctgcgttttc aacagccata cggatcgctg cttcatcttt tgcatcgtcc gctgcttttt
    20461 cttttgtttc tactaattct ttgcgatatt tttctttcaa ttcgtctaat gttgatactt
    20521 cgtcatcaac atcttttgcg aattcgtcat ctaattcagg cacttctttt gcttttactt
    20581 catggattgt cactttgaag actgcttctt ttccagcaag atcttctgct tgataatctt
    20641 ctgggaaagt tacatttaca tctagacttt cacctgattt atgaccaact aattgatctt
    20701 caaaacctgg aatgaatgag cctgaaccta attcaagtga gtaattttcg ccttttccgc
    20761 catcaaacgc ttcgccatct acaaagcctt cgaagtcgat tactactgta tcgccatttt
    20821 cagctgcagc atcttctttg ataactaatt cagcttgtgc ttcttgttca cgtttgatac
    20881 gtgcatccac gtcagcatct gttacttcac ggtcttgttt ttcaacaact agatctttat
    20941 attgtcctaa ttttacttct ggtttcactg ttacttcagc agtgattacc cagtcttgac
    21001 ctttttccat gctttcaaca tcgatttttg gttgagaaac aggatcgatg ccagcttctt
    21061 tgacagcagc ttcataagct tctggtaata cagcattcaa tgcatcttca tataaagctt
    21121 cttcaccgta catacggttg aatacttgac gagaaacttt ccctttacgg aatccaggta
    21181 cgttcaagct ttttttcact ttattaaacg cagttgttaa tcctttttgg atttcgtctt
    21241 gcgcaatcgt aaattttaaa acaccatcat tcgtgccttt tttttcaaat gttgcagtca
    21301 tttaattccc tccgagttta gaaattcatt ttatacatca aaaacgtttc atgcatacct
    21361 ttaaatagta cactaaccct ttcaaattgt aaacatattg aatggataga atccttgttt
    21421 ttcataacat ttttatcttt ttcactgata aaatctaatg gaagaagatc atttgctgca
    21481 aaattttttc tctttttttt aaaaaatctt gtttttcatc ttctgtacct tcatacatcg
    21541 tttttcctgt acacgcctct acagtcagtt gaaaccagcg cttcgggtct tcgatgtatg
    21601 tatcatagac aggataaaca attgcagatt gcaacgtgaa ttcttcattt aacacggaac
    21661 taagcagcgc atcttcatta ccgtaacgat ctgaaaatac acataattgc tggtaacttg
    21721 cctgctgctg cggctttggc agatcttttg gtatgacttt gactaacttt tcttcaatcg
    21781 tcaaatagaa aacaggatgg cattcgttca attgcctaag tgtctccagt aattttgccc
    21841 ttgctaaaat atggacctct ggcgatacaa taagtttact cgctgtttgg ataaactcat
    21901 gataaggaag ctgttcgatc tcatcaatag acgcaagctg acgtgcaggt tccaacgaag
    21961 gaagttcctc cgatttattg tggagtgcct tgatcttctg cctctccatt tgtccggata
    22021 attcttctgc aaattggatt ttttcaatga cccgctgctc gaaagcttca taaaaatctg
    22081 tacttgctat caattttcta gcatataaaa aatcttgtgt actgattaac aagtcaaaat
    22141 aaaattcagc aaactcctca ttttgaagat actgtttttc atgcaagagt gcttgcttca
    22201 atgcttcttt tttctcgtcc aactcgtaaa gacaaaaaac aagcaggcta ttagcctcaa
    22261 agctttcttt caactcgtaa gctcgttgaa ataattcttt tgcctgctga tattgctggt
    22321 ttgctagtgc ttgttgtgct actcgcatta aattctcata tttttctgga aaatcaatcg
    22381 gcttcacgca gtgtcctcct cttgctcatg ttcagctcat gttcaatagt agcaaaacaa
    22441 aaaaagcatg acaattaaaa ataactgaag caccctctaa aagctagatt ttagatctga
    22501 cttttagaag gtgctttcaa ctgttactgc tttttattgc tcatcagctg ttgacttcct
    22561 ggttgaatgc tttgatcaga ttgatcgtaa caacacttgt attagtgaaa aaatcgccta
    22621 gatgttgctt tttctgatta aagattgctg gaagataccc taacatcagc aaaggaaatt
    22681 ttagaatgaa ccggcaagca ccttctctaa ctaaaacggt tgaccagcta agttcttctt
    22741 catcaaagct gatgactcga ataccaaaga tcattttccc aatcgtttga ccatgattga
    22801 atttcgtcaa taaaataaaa tacgctagat aaataagtaa ggctgtgaat ccgtagatac
    22861 tcagataaga atctgaaaaa ttaagccaac ctagattttt ggcaatacca agtgttaaac
    22921 gcgtaatagc tccgatacac aaaagatcga ccaagaaagc aaacattcgg ataaaaaaac
    22981 ctgcatagaa atacttagga aaatcatttg cttttggcgg ttcatttctt gtgtccgtat
    23041 actttttcca tttacgttgt ttctttttga tttcttcggg cgtcaaaggc tctctttcaa
    23101 aagagctcag taccttttgg gaaaagttgg agtcaggtcc tacagcaggc tgctgttgct
    23161 tatctttttc ttgttcctta ttttcatttg aaagctctga catgcttact cacctccgta
    23221 ataatacatt gcttttggtg attccgctgt ccctagattc tcaagaacgg ataagatctg
    23281 actagtctca gaaggcttca atccctggaa ttctgctagt ttacttccta accatgtact
    23341 agcaaaccca gatggcgtgg aagaatattc tactagttcc gcatcttcaa gctgttgttc
    23401 ctttcgcata gcttttaaag catcttcagg aaatcccaat tcatcgacta aacctacttc
    23461 ttttgcttgg acaccatcat aaatccttcc atcagcaatt tttttgactt cttcttcaga
    23521 tttattccgt ccctcgctga cgattgctac aaaacgacta tatgcgctgt cgatatatgc
    23581 ttgaagcacc gcttcttctt cttttgtctc tggtcgcgtt cctgaaccca tatctttcaa
    23641 cgctccgctt ttaactgttg tatcttccac acccagtttt tctagaagtc ctgaataatt
    23701 caatcctgac atgatcacac caattgagcc ggtcactgtt tcttctgttg caaagatttt
    23761 atctgcttga gcagaaatat agtagccacc gctagctgcc atgtttttca tgctgacata
    23821 cattgggata tcatgttctt tacgaatcgt atccaatttc ttagcgattt ccgcactttc
    23881 gtagacaccg ccaccaggag aatttacttc taaaaagata cctttgactg ttggatcttc
    23941 ttcaatcgcc ttcagttgtt ccataaaatt tttatgattg taaccttcag aagcaaataa
    24001 acctgaagac cctcccgcag caatcgttcc atctactgat aattttacga ttttttgcgt
    24061 actgtctccc tcttccaaca catttgaagc tagttcttca tccccatata accaactgtt
    24121 caatccctca atttgatcgt tttcctgagg cttagtcaaa ctagctgaaa ctaacgaaac
    24181 cacaaataat cctgcagcaa tcagaactgc tgcccaacgt ctcttattca ttctttctcc
    24241 tttctaatac aagtttttta tgacgatcac agtttctgac tgctcagaag tgtcgaaccg
    24301 ctctgctttt tcttcccgcg catacccgga aaacgactct tttgtcaatt catcttctac
    24361 cgtaaactca cgagcttcag caacttccaa ggcatcaggt gcgatttctt ggatgctatc
    24421 ccagccagta tctaaaaata ttttcgtatc atctgataca ccagctgatg cttcgaattt
    24481 ggacaattgt ttttttaaca ttcccagtgt catgatctca atttttgaat ccatttttac
    24541 tcctcctcta tccgattact ttctattgta ccgaactcct gaaaaagatg atagataaaa
    24601 acccattatt ttttgacata aaaagagcat ttgacaaagc atttgatata taccaattta
    24661 ttttttattc tttttttgtt gacaagaaca ttcgcaaaag gttatgattt taaatgtttc
    24721 cagataaaaa acaaaagagg aggatagtta taccttttag gatagcgcca ggtctgtgta
    24781 aacagatgac gaggagaggg tttatcgaaa gattcggcgg atggccctag ggactgcact
    24841 ctacagaaaa tccacaaaaa atatctgcaa agataaaaca aaaggatttt cctgcagctg
    24901 gaagcattca tacgaattca ttatagaaag taggatgttt tttatgtgcg gaattgttgg
    24961 aatgattgga ttaaaaaatg taacaccagg attgattgac ggtctagaaa aattagaata
    25021 ccgtggttat gattcagctg gtatctttgt ctcagatggc acaactgatt atctggtcaa
    25081 agcacaaggc agaattcaaa atttgaaaaa caaaataaat attgatacta caggaaatat
    25141 aggtattggt catacacgct gggcaactca cgggcaacct tctgaagaaa acgcccatcc
    25201 acatacttcc caaagtggac gcttcgtgct cgttcacaat ggcgtaatcg aaaactttga
    25261 agaacttaaa attgcttatt tagcaaatga ccactttatt ggtgagaccg atactgagat
    25321 tatcgctcat ctaattgaaa ctttcgcgaa ggatactaca acgcaagagg cgtttttaaa
    25381 agctttgcgg gtcatcaaag gttcttacgc tttcgctttg attgatcgga cagcacctga
    25441 tgttatttac gtcgctaaaa ataaaagtcc actattaatc ggactcggcg atggattcaa
    25501 tgtgattgcc agcgatgcaa tggctatgct tgctcatacg aaggaattcg ttgagattga
    25561 agatgaagag atggttaccg tcacatcaga aaaagttatt attcaaaatt ttgcaggtga
    25621 catcgtagaa cgttcttcat tcgaagcaca agtagattct tcagatatcg agaaaggtac
    25681 ctatcctttt tatatgctga aagaaatcga cgaacagcct attatcatgc gtcgtatcgc
    25741 tcaaaaatac gtaacagaag acaatcgtgt ctctcttgat caaaagctgg tcgatacatt
    25801 gtctgatagc gatcgaatct atatcgttgc ttgcggaact agttaccatg cggggttagc
    25861 aggtaaacag actctggaaa aattgacaca gatccctgtt gaggttcatt tagccagtga
    25921 atttggctat aacacacctc ttctttcaaa aaaaccattc tttattttct tgagtcaaag
    25981 tggcgaaaca gcagatagcc gccaagttct ggtaaaaatc aaccgtctcg gctacccttc
    26041 acttacgatt acaaatgttg ctggttctac tctttcgcgg gaagcatcct ttacactgct
    26101 attgcatgct ggacccgaaa tcgcagtagc atcaacgaaa gcttatactg cacaaatagc
    26161 tgtactaact ttattagcca aagctatcgg tgataaaaaa gaatttgaaa cttctcttgt
    26221 attcgatatc actcatgaat tgagtttagt tgctaatgcg atggaatcgg tcatcgccca
    26281 aaaagattat ttagaagagt tggcagaagt atatctcagc gatacaagaa atgcctttta
    26341 cattggacgt ggcgctgact acaacgtttc tcttgaagct gctttgaaat tgaaagaaat
    26401 cagttatatc caggcagaag gttttgccgc tggcgaattg aaacatggaa cgattgcttt
    26461 gatcgaagaa ggaacaccag tgattggaat catcagtgaa gaagtgactg gtgcgcatac
    26521 tcgaggaaac ctgaaagaag tcgaaagccg tggcgctaaa acactagtta ttgtctcaga
    26581 gggattagaa aaggaaagtg accagctggt gctaccaact gtccaccctt atctaactac
    26641 tctaaccaca gtcgtaccga ctcaattact tgcatattac gcaacgctat tacggggatt
    26701 cgacgttgac aaaccacgta atttagcgaa atcagtgaca gtagaataat aaaagaacaa
    26761 ggaccagtgt cagacatctg acactggtcc ttgttctttt attttttatg aaaatatctc
    26821 ttaatccagt ccgatttctt ctttgactac tgccgcgatt ttatcgacat agtaattaac
    26881 tttctcatca gtaggcgctt ctgccatcac tcgaagtaat ggttctgtac cggaaggacg
    26941 aactaggatt cttccttctg atcccatttc agcttctgcc tcttcaatca cttttttgat
    27001 tgccggaaca tccatcgcac cgtttttatc acttactcgg atattcacta atttttgcgg
    27061 atagattgtt acctctgctg caagctctga taatttctta cctgtctgtt tcatgatatt
    27121 cagtaactga attcctgaaa gcatcccatc tccagttgtg ttaaaatcta agaaaatcat
    27181 gtgcccagac tgctcgccgc caaagttata atcgtttttg cgcatttctt ctacaacgta
    27241 acgatcacca acttgtgtaa cgacatcttt caaaccgatt ccttccaccg ctttgtggaa
    27301 tcctaagtta ctcatgacag tcgtaacgat cgtatctttt ttcaatcgat ttttttctgc
    27361 caagtattta gcacagataa acatgatttt atcaccgtcg acaatgtttc caagttcatc
    27421 caccgcaata atacgatcgc catcgccatc gaaagctagt ccagcatctg ctcctttttc
    27481 aacgaccatc tcagctaaac gttctggatg ggtagagcct acgccatcat tgatatttaa
    27541 cccgtctgga tttgtgccca tggtataaaa atctgtttca agatcggcaa acaaacgatt
    27601 aacacttgtt gaagttgctc cgtttgctgc atcgacacaa atggtcaaac cggaaaggtc
    27661 accgttgatc gtttgtacaa ggaattgaga atatttcaat aagccttctg gaaattcttc
    27721 tacagttccc aatccttcag ctgatggacg aggcaattca tccacttcag catctaataa
    27781 tgcttcaatt tctagctctt ggtcatctac tagtttaaaa ccatctgcac cgaaaaattt
    27841 gattccatta tcttgtgctg gattatgaga agcagaaatc atgacccctg cgcttgcttt
    27901 ttgcaaacgt gtcagataag caacacctgg tgtagagatc acacctagct ggaacacttc
    27961 gatacccacc gataaaagtc ctgaaatcag cgcttcttct aacatttgac ctgagattct
    28021 tgtatcccgc cctactaaca cgcgtgggcg ttttccttct tcttcatgct ggcttaggac
    28081 ataacctccg caccgtccca gcttaaatgc aagttctggc gtaagttcct tgttggcttc
    28141 accgcgtacg ccgtctgttc caaaatattt acccatagtt gtaaaattac tcctttaact
    28201 taattttctg aggaatttgt tctgctcatt tgtgttgatt cgcttgtttc tgatgtttct
    28261 tctgtacttg tggtcgagct gcttgtccca gattccgaac tggaagaaat ggttgttgtc
    28321 gatggttccg ttcctgaatc agcagatgaa atacttctac tcgtacttgt attttcttgt
    28381 gtccgactgc tcgtcgaagc agagctactg cttgacgttt gcaacacggg tgtaatctcc
    28441 acactaactg attcgggctt gatacttaac ccctcttcta ctggtatttc aattttacga
    28501 gttgttcgat gattgatacc tgagacattg atcggtacag caatactgtc accgatcgat
    28561 gcaagcaaat cgctagagcc gcttacttct gcttccagtt ttgaaaaact gaaatcatag
    28621 ctttctaccc cggcagctgg tgttccttgc tgtatcccat aaagtcgtac cgacttgctt
    28681 ggcttgatcg aatcagctct cactgttact tgcactggat cagagacgat acttaatact
    28741 tcccctgctg cattcagcgc ctgtattggg acctcactat taatcccatc atctgtgacc
    28801 atcgaagcgt ccacatcagc tactactcgt ttgatttctg ccaatgtttt gtcacctgta
    28861 gtgatagaga cttttttagg actaacggaa gtatcatcaa gttcatatcc agatggtgtg
    28921 aacgttgaag gaatcaacgt ttccacatcg aaatctttta ttaccttttt ctcaaccgtt
    28981 actgtaatag tcgatggttc gatctttgct gtgacagcac tgctcaaatt ttgaattttt
    29041 agcggaacct catgtgttcc ttcgcccagc ttagtgatat ctgctgtgac gcggaacatc
    29101 cgtgtatctt cgtttgcctc tgcattcaac tgaactcggt ttgcactgga aagtttgaca
    29161 gtcactgcat tttcatatcc atggatatag tagcgctttg aatcataaga aatcttgatt
    29221 ggcaccccag taactgtttg attatacgct tcatttccag aaagcgtatt tggaacggac
    29281 tggccatttg cattgaagta cagcgcaata ctgaacagca atgcaataaa actgtaaaga
    29341 atgcttgtcc gtttctcttt tgagatcatt tgcgatcccc tcccttagtc ataccgtcta
    29401 ggaaagattg aatcacgttt ttcttgtttt ttgttttctc ttcagcaatc agttctctac
    29461 gcaaaattgc caaatattct tgctgggata agccagccat cagttcatta tctaatgtaa
    29521 tgctgacatc cccagtttct tcagagacaa cgatggtaat cgcgtcgctg acttcgctta
    29581 taccgacagc tgcccggtgt cgagtcccga attcttttgg aatcaacata ctttctgata
    29641 gaggaagata ggcgcttgct actgcgatct ttccttcttt tacaataact gcaccgtcat
    29701 gcagcggtgt attaggaata aagatattga tcagcagctc tccagtgata tctgcatcta
    29761 atgcaattcc tgtctcaata tactcatcta atccagtatg ccgttcgata gtaattagcg
    29821 caccgatttt tcgcttggac atatattgga tggctttgtc aaaagacaag atcatcttct
    29881 catcttcctg ctgttcgctt tttgattgtt tgaaaagcga actgcgtcca agatgttcca
    29941 gtcctctccg gatttccggc tgaaaaatca cgatagcagc tatcaccccg tacgtaatga
    30001 cttggttcat cagccaagac aaggtgtgca gaccaatgat ttccgctacg attcggataa
    30061 caataaaaat agctacacct ttcagaagct gaatcgcttt tgtgcctcgc agcagttgga
    30121 tcagtttgta tacaagatac cagacaacca gaatatcaag tatgttcacc aaaaaattca
    30181 gagagaacaa gtcaccggaa aacatttgct gccaatagtc caaattaaaa agttccccga
    30241 tttgaaatga cataaaaagc ctctcttctc ttttgagatt tcctctctag tatatcatat
    30301 gttttaattt tagaggctga tctgctcgta tttttcttga gaaaaacgca taattttcag
    30361 gaaaattgca agtatgcgtt ttatttttct ctgcaataaa tatttttaaa cttcagagaa
    30421 ttattcatta tattttttct gttatttttc ctttttttct tttgtagctt gttccatttt
    30481 tttacgtcgt tctgctctgg cagctcttct ttcttctgct tcaaggtccg tcgttttttc
    30541 ttttctagtc ttaacgggta catcgataga ttcctctggt tcaagtcgtt tcttgatttt
    30601 ttctaattct cccgtcagtg cagcatagtt gtaaaaacgt gttttagcgt cttggatagt
    30661 cttttcaaat aaagcttttg caagatttgg atttgcgttc tctagcgagg aaaagcgaac
    30721 ttgtttcctc atgaaatcag gcatagcaga gaaatcaggt tttttgtagt ccaaagtcat
    30781 tgggttcttg tttttttcga ccattgcagg gttatatcga tataatgacc aatagccaga
    30841 agaaacagct tcttttgctt ctttaagcga ttctttcatt ccaccaagca aaccatgtgt
    30901 gatacaaggc gtataggcaa tgattaaaga aggtccagga tacttttctg cttcttcaaa
    30961 agcctttatc gtctgcattt gattggcacc tgaagcaatt tgcgccacat aaacatttcc
    31021 atatgtcatc gccatcattc ctagatcttt ctttgaagca tactttccac tagccgaaaa
    31081 tttagcgatt gccgaagctg gtgttgcttt tgaggtttga ccgccagtat tggaatagac
    31141 ttcgttatcc aaaacaagaa tattcacgtc tgctccgctg gcaagtacat gatcgatccc
    31201 gccaaaacca atgtcgtaag cccatccgtc tccaccaatc atccattggc tgttttttac
    31261 gaatagatcc tttctcttgt atatatcctc cagttcaggg acaattgatc gttcgctttc
    31321 gatcgcagct tgcagttttg ctgctctttg ctgcgttcct tcgctctcaa acatgtgtac
    31381 caaccagtct tccatcaatt ctcgtagttc ttctgatact tttggcaata cttctttgat
    31441 tgaacttgcc aaagcctcgc gacgagtctg tgccgctaga agcataccat aaccaaattc
    31501 agcattatct tccaataaag aatttgacca tgcaggccct tgaccacatt cattggtcgt
    31561 ataaggagaa gcaggagaag caccgcccca aatagaagaa cagcctgtcg cattcgcgat
    31621 catcattcga tcaccgaaca tttgtgtcaa taattttaca taaggtgttt cgccacagcc
    31681 tgagcaagcg cccgagaatt caagaagcgg ttgattgaat tgtgtagaca agacagtatt
    31741 ctttcctttg actggatttg ctttttgtct taatgtcatt gcaaaagccc aattcacagc
    31801 ttgttctttc tgttcttcat aaggcttcat cactaaagct ttttcttttg cgggacaagc
    31861 ctctacacac agaccacaac ctgtacagtc ttccactgaa acttggatac ggtattttac
    31921 gccgtctgcg cctcgcattt ctctcacgat aaatccttct ggcgcttctt ccatttcctc
    31981 ttcatctgct aaaaatggac ggatggcagc atgaggacaa acaaaggcac attcattaca
    32041 catcgtacag cgatcactaa tccactcagg tacttctaaa gcgacgcccc gcttttccat
    32101 agaagccgtt cctaaaggca tttcgccagt tgtcattcca tttgctacaa gtgtaccaac
    32161 agagagttga tttccttcct gtgcattcac tggttcaagt atttcttgta tataaccagt
    32221 gttcctttta ttgatttttt caggcatctc caacgttctc caatggtctg ggatcgcgac
    32281 ttgatgaagc agttcggtcg tccgatcgat tgcttgtatg tttttttcta cgatagccat
    32341 tgatttatgt ccatagcttt tcagggcttc ttcttttagc agcggaagca cctcatcaaa
    32401 aggaatgatg tcagctaatt taaaaaaagc cgtttccatt gccgtattga tccggcgtcc
    32461 caaaccgact tcttgggcca atctcatggc attgatcgta tagaatctaa tgttattttc
    32521 agccaagtaa cgttttaatt ttaagggcag atgcgtttct aattgttcgt cgctccaaag
    32581 agtattcaat aaaaaaatgc cacctggctt caatcctttg accaagtcat agctatgcag
    32641 gtaagctggt gtgtgacagg caacaaggtc cgcatgctcg acaagatacg ctgaacgtat
    32701 cggcgtatct ccaaaacgaa gatgagagac agtcagaccg ccagattttt tcgaatcata
    32761 ataaaaatag ccttgcgcat atttatcggt gtgatctcca ataatcttga tagctgattt
    32821 atttgctcca actgttccgt cagatccaaa tccccaaaac ttggcctgga atgtttgcgg
    32881 ttctgtcaaa tccagacttt cctttggttc caaagattga taagtcacat catccacgat
    32941 ccctatggta aaccgttttt tgcgtatgct tgggtctttt ttcaattcat caaaaactgc
    33001 tgagatttgg tcgggagtca catctttaga ccccaatccg tagcgaccac caataacagc
    33061 cgggcgaagt tcagaatcat ataacgcact ttgaacgtct aataagagag gttctccacc
    33121 tgcccctggt tctttacttc gatcaagaac agcaattgtt tttactgtat gaggcaactt
    33181 ttgcacgaat gtttccactg gaaaaggacg ataaagatgg atattcaaaa aacctgtttt
    33241 ccgtccgttt gcattcaagt aatcaatcgt ttgttcaatc gtctgtgcaa ctgatcccat
    33301 agaaacaatg acttcttccg catcgtctgc tccgtaataa gtcaccaagt cataagaagt
    33361 tcctcgtaat cggttgatct ctttcatata tttttgaacg atttcaggta atttatcata
    33421 ataggggttg atcgtttccc gttgttgaaa atggatgtct ggattctggt tcgtaccact
    33481 aacagtcgga tgattgggat tcatccctcg tttacgaaat gaagctaatt tctcctgatt
    33541 gatcagagga gctaattctt catattccaa aacttcgatt ttttgaatct cgtgactcgt
    33601 acggaatcca tcaaagaaat tcaaaaaagg tacttctcct tcaatagaag caagatgggc
    33661 aacaggtgct aaatccatca cttcttgtac actgctttca gctaacatag caaatcctgt
    33721 ttgtcttgca gccatcacgt ctccgtgatc tccgaaaata ttcaacgcat ttgtcgtcac
    33781 tgcccgacta gcaacgtgga aaacacttgg cagtagttcc ccagcaatct tgtacatatt
    33841 cggtatcatc aaaagcagcc cttgtgaagc ggtatatgta gttgtcagtg caccagtttt
    33901 caatgatcca tggaccacac cagctgctcc tgcttcggat tgcatttcta ctacttttac
    33961 tggttgaccg aatacgtttt cttttcctgc tgctgaccat tgatctacta attcagccat
    34021 ggtcgaactg ggtgttattg gatatattgc agcaatctcg gtaaatgcat aagaaatata
    34081 tgcggctgcc gtattgccat ccattgtttt catttttcgc atgattcatc atccttgttc
    34141 atattctctc ttattatctg ttccattata acgtaaaaca tgccattttt ttcttttatt
    34201 tataaaaata ttttacatgt atctatttga attggtcatc taatttttag acaaacagtc
    34261 taaatcgttg cctttttttg tcagacatgt ataaactata agcgaaagag gtgttttaac
    34321 atggatatat cagtgattga tgcaacgaaa acaaatacac aaaaaggaat cctttatggt
    34381 tcatcgaatg ctcctaaaaa aatggtcgaa ttcatcaact tagcttgtcc gtattgccga
    34441 caatggtttg aagaatctta tgagctatta gaagaagcag tccaaagcgg gcaattgcag
    34501 cgagtgatca aattgttcga taaagaaaaa gaaagcttac agcgagggaa cgtgatgcac
    34561 cggtatctca caatcagcga tggccaaaaa gcgatcaaag agatcaaaca aatattcgat
    34621 actcaagacg agtggaaaca tctttctctc caagaagtag cggattttgc agtagataaa
    34681 ctaaagctga cagaacaaaa agacgaacag ctgagccaag ctgtcatcaa cgaagctgag
    34741 caagcacata ttcgatttgt tccaactgtc atcttgggta aagaaatctt tgatgaatct
    34801 atttcaatag aggaattaaa agaattgatc caaacaaaat aaaaaaataa ggagcgtggg
    34861 acaaaagtaa agaacacttt tgtctcacgc tctaaacacg tataaacggc ggaagcagaa
    34921 gcaactcctt cttatttctt ggagttaaac gcttctgtcc cagcctcctt attccgaata
    34981 aatggtgtcc aaaagcttgc atctgccaga ttatctcatt tcttatgaca agctaagcaa
    35041 attatctcca ccaaataatt tattcatatt tttgacttgc gcatactaac taattcttaa
    35101 attttcatac aaaatttctc aatttccctt aacgagtaat atttatgtac ggtgattctc
    35161 actagtatcc caatcacttt ttaagttatc ccgatttcaa ttctacttca aaaatagttg
    35221 cttttatgta tgctgttagt tgattagatg gtcatttgtt gagctaggtc gtttatttta
    35281 aacaagttgt ttctttagct tatacctatc tcgtctacca aacgcaagat ggcaaacaca
    35341 aatgcatacg catctgtatt tgccactttg attcgtataa agctaacaac tattaactcc
    35401 tctcagctaa tagttgctct cttttttatt cagattcatt tttgctgatc acagcctagt
    35461 atttcaatgg ttttgaacac taaaccaaat tgttccatta caacattcac tggctgatga
    35521 aatggattag tagatttcga ttattcaaag cgattttcta ttggtggatc gtgttcccaa
    35581 ctaataagac ggattccaaa cctatcgtac agatcttcta aaccacttat aaattttgca
    35641 cctatttcag gtgttttatt gcgtggatat tctattgtta cgccattaaa tggataccaa
    35701 ttaggattag gctctctttt tttctgtaca tctcccatag aatagttagc actttctggg
    35761 acataatata ttcttcgacc atcctctaat tcatctaata agatatttcg tgatcgtcta
    35821 tcacttgcta aatgtagacg ccacgtgcga taccatgcca tgctacctgc atctgcagaa
    35881 aatccatcaa ataatgcaac aattgtatct tctatcacaa tcctttcccc atctacttca
    35941 tattctaatc taaaatcaaa ctcccctcgg gtaatttctg gtcttggtgg cttaggaccc
    36001 aatattattc tacttaaaac cgcattcgtt cccagatgaa acacagctat aacaacaata
    36061 atgattaata ttttgtttct atttgataaa tttctcatta ttcgcacatc acctctacat
    36121 tctcacatat tagttcgtaa tactccttta atatcaaaat atctactggt cgtaataata
    36181 ttcgctatga attcctacta ctcgtataag aaaggccaaa tcatcagctt ttacttgctg
    36241 atgatttggc ttgtttctat ttatcgtaat tgttttctaa tcaccaaaat tgttaacaat
    36301 cggttcagat atttcccagt tgattaaggt aattccgtat tgtgtcaaaa cttcttgcgc
    36361 ttctccgaaa ttaagtacag ttcttatgta tccactttct cctaatataa agtctacctc
    36421 tcttttaaaa ccttcctcga gatttctaag ggctatatgt ggttttaatg acattgattg
    36481 tacattttcc cccatataat attgcgcact tcctcgacta aaatagattc ctagatcgtc
    36541 cccaaaaggt agatcttctc taccactagc taatctactt ctccatcttc gttctctttt
    36601 catagaacct gcgctgaact cacacccgtt aaactcagct atgacagtat cttcaattat
    36661 gttaatctgt ccatctagtt cgtattcaat cctaaatgga aattctccat aggtgatttc
    36721 cggttcgggt attttaggtg agattaatgc aaagaaagga aatattaagc ttggcaacat
    36781 caaaaaaact cctcctatga tcacaattcc cgatgtaatc caaaatataa tttgtacaat
    36841 tctcattgat ccattgtatt ccattttatt ccccctatta ctattcatat ccgcctatct
    36901 atatcgatct tccaatgcag agataattgc ttccatacct tgatttttta ctgagttacc
    36961 tatgcgagat atcattctcc actaaggtgc atcatattgt ctatctaaca tgattaattc
    37021 tccaataggt gtatactcct ccaaaaacaa catttaatgc atctccctcg acaacataag
    37081 ctaccatgtt tgctgtatat gtgtttgagt ctaattcata agcagataaa tttactgtcg
    37141 ctggattaaa tataatcgca ttcgtattta tagctactga atttgctgct tcagcaccac
    37201 cttttgaatg tccgatcatt gtgatttcaa tagtaggatt tacgtggaca gccctcaccg
    37261 tttaccctga ccattataga aatcgcttac ttttttgaac tacgcaacaa aggatacaat
    37321 gctcctaact tcactgtctc catttttgtc cactaaggaa tataaaaaat aggcattaat
    37381 tcatttaatt cggtcacaac acgttacctt ttaaatcatt atactgtatc tcatacatta
    37441 ttatcaataa actattataa aaatgcaaaa aatgctttat caatctattt tgtaattggt
    37501 cataatatca ttgaaatcat accctgtatc gatttttcct atgaatctcg tctatttgtg
    37561 attgacgaaa aagaggcaag acagacactg gaatggtaat tttacccact attttgctcc
    37621 aactatttgt aatcgtaccc acttttgaaa taccgagatg atcattttaa ttgatatgct
    37681 gattcccaag caaaacccca agaaattaaa tgctctatcg catctagctt cttgggtttt
    37741 gcttatataa attccaacat ttaaattttt tatgctgttc ggtctcagaa atgccgtttt
    37801 tgcttatatc acaatatcaa gatgtcaaat tattcgtgtt gccgatgact ttgattaatt
    37861 tttttcaaat tgtttattaa gactaatgct gtctttttgc agataccaat gttcgaactt
    37921 tttaacatta tcatatgatc ctgcaagttt taacttctta ccattagatg ttatcaaagt
    37981 tataattaac cttgcctttt tcccattagg atcttttatt ttatgaattt tctgcaggta
    38041 atctctccta atggcagcta tttttacctc atcagtgctg ataaaccatt gatcatccac
    38101 atacatactg cctgttatgt tgtgtttttc catttcttcc gagaacgaaa agtttaaggc
    38161 tcgttcttac ttccaaagtt gaatgagtcc atggattgga cttagtgtga gaattgagaa
    38221 caacaataag aatccatatc ctcccgcaaa catccctaag gctatactgt ctctccatcc
    38281 ataagtagga ttagaagaga aaagagtcac cgtttgtaat aatatgaaaa ggaataatcc
    38341 agcacaaaca ccaaagataa caactccaaa cattgaattt ttatacaatg accatttatt
    38401 tataggcttt tctttcattt tacttccccc tactgttctg ttcacacgga atgaattcaa
    38461 agttcataag ccctcctctt tgaacatatg aatacgactt gctaactata ctttaaaaaa
    38521 gcttgtttta agcggttata ggcgagttgt aatctctatt catctcatta gcaaataact
    38581 tgccgaaaag tctactgaaa ttaaagcaga tagtggaaga attatttagc caggatagac
    38641 actatttgtg aaacacatgt ctttactttt gaaattctat gatgaaaagc cttggaatac
    38701 agtagctctt aattggtaat actttttcca ttataaataa cctcttttca ttttatattt
    38761 atcccctcta aaaataatat ctaatctaat attttaaact tatatttccc tcgctgtgct
    38821 aatagagcat tactaaataa atagaaacta gaaaaaagca ttacgcctgc cagaataaac
    38881 caattatttg t
//
LOCUS       DACDXO010000021        37077 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E23605_2017
            SAMN12639989-rid9881463.denovo.021, whole genome shotgun sequence.
ACCESSION   DACDXO010000021 DACDXO010000000
VERSION     DACDXO010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639989
            Sequence Read Archive: SRR10023389
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 37077)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37077)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741914.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:42:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,972
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 33 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 17 of 73
            Pseudo Genes (multiple problems)  :: 10 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37077
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639989-rid9881463.denovo.021"
                     /isolate="E23605_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..671
                     /locus_tag="IWM35_001249"
     CDS             <1..671
                     /locus_tag="IWM35_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP9919110.1"
                     /translation="LQGAVFELQDANGTVLQSGLTTGADGKLAIDGLAPGVYQLVETQ
                     APTGYELDATPIEFEIERSQTAVVELTKENRLTPGGVVLTKIDDQSGEVLQGAVFELQ
                     DANGTVLQSGLTTGADGKLAIDGLAPGAYQLVETQAPTGYELDATPVTFKIEKEQEVA
                     IFLTKENRKVADSSDIRGNNPTSSGNKHLPKTGETIISQFILSILGVLLVFISIKFRK
                     KRNI"
     gene            complement(1095..1265)
                     /locus_tag="IWM35_001250"
     CDS             complement(1095..1265)
                     /locus_tag="IWM35_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP9919111.1"
                     /translation="MPRLTNYFLSCTNPKEIEFLVTDMNATYFQLTKHVLPNAKLVID
                     RLLGFSTPFKEA"
     gene            complement(1459..1638)
                     /locus_tag="IWM35_001251"
     CDS             complement(1459..1638)
                     /locus_tag="IWM35_001251"
                     /inference="COORDINATES: protein motif:HMM:NF026041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9919112.1"
                     /translation="MPMVIRLKKQRYTCKNCRSHWNAQSYFIRPRHSISNHVRHKITS
                     LLTEKVSLFFISKSC"
     gene            complement(1648..1824)
                     /locus_tag="IWM35_001252"
     CDS             complement(1648..1824)
                     /locus_tag="IWM35_001252"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9919113.1"
                     /translation="MITEVSYETLQKKKTLIVAAVLFPAPCVCRNCGSAVVDGNRKLM
                     VVKNKKKETIVHFE"
     gene            2025..3137
                     /locus_tag="IWM35_001253"
     CDS             2025..3137
                     /locus_tag="IWM35_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAP9919114.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            complement(3215..3508)
                     /locus_tag="IWM35_001254"
     CDS             complement(3215..3508)
                     /locus_tag="IWM35_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HAP9919115.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            3676..3990
                     /locus_tag="IWM35_001255"
     CDS             3676..3990
                     /locus_tag="IWM35_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HAP9919116.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            4011..5990
                     /locus_tag="IWM35_001256"
     CDS             4011..5990
                     /locus_tag="IWM35_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAP9919117.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            6003..6329
                     /locus_tag="IWM35_001257"
     CDS             6003..6329
                     /locus_tag="IWM35_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HAP9919118.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            6345..6863
                     /locus_tag="IWM35_001258"
     CDS             6345..6863
                     /locus_tag="IWM35_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9919119.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            6863..8215
                     /gene="celB"
                     /locus_tag="IWM35_001259"
     CDS             6863..8215
                     /gene="celB"
                     /locus_tag="IWM35_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HAP9919120.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(8299..9804)
                     /locus_tag="IWM35_001260"
     CDS             complement(8299..9804)
                     /locus_tag="IWM35_001260"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HAP9919121.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLKFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(9896..10486)
                     /locus_tag="IWM35_001261"
     CDS             complement(9896..10486)
                     /locus_tag="IWM35_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP9919122.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            10665..12311
                     /locus_tag="IWM35_001262"
     CDS             10665..12311
                     /locus_tag="IWM35_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAP9919123.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYLQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            12360..13079
                     /locus_tag="IWM35_001263"
     CDS             12360..13079
                     /locus_tag="IWM35_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HAP9919124.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            13558..14505
                     /locus_tag="IWM35_001264"
     CDS             13558..14505
                     /locus_tag="IWM35_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP9919125.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLASLRESPWVGWENFKFLFSSDQAFLITKNTILYNVAFILLNLLIS
                     VVFAIIMSELRNKRLVKVYQTMSLLPYFLSWVIISYFVYAFLSPDKGIFNQWITGHGG
                     EAVNWYNEPKYWPFILVFIGTWKGIGYNSIIYFASVMGIDPTYYEAAMVDGASKWQQI
                     KHVTIPQLIPLMTILTILAVGNIFRADFGLFYNIPRNSGALYDVTQVLDTYIYNGLTS
                     TGDFGMTAAAGLYQSVVGFVLLMITNTIARRFDSDSALF"
     gene            14517..15437
                     /locus_tag="IWM35_001265"
     CDS             14517..15437
                     /locus_tag="IWM35_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP9919126.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            15478..16935
                     /locus_tag="IWM35_001266"
     CDS             15478..16935
                     /locus_tag="IWM35_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP9919127.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            17280..17894
                     /locus_tag="IWM35_001267"
     CDS             17280..17894
                     /locus_tag="IWM35_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAP9919128.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYLSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            17887..19611
                     /locus_tag="IWM35_001268"
     CDS             17887..19611
                     /locus_tag="IWM35_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP9919129.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELRELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            19608..21059
                     /locus_tag="IWM35_001269"
     CDS             19608..21059
                     /locus_tag="IWM35_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9919130.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
     gene            complement(21532..23685)
                     /locus_tag="IWM35_001270"
     CDS             complement(21532..23685)
                     /locus_tag="IWM35_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HAP9919131.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDREYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
     gene            complement(23687..24733)
                     /locus_tag="IWM35_001271"
     CDS             complement(23687..24733)
                     /locus_tag="IWM35_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP9919132.1"
                     /translation="MTQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            24883..26172
                     /locus_tag="IWM35_001272"
     CDS             24883..26172
                     /locus_tag="IWM35_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HAP9919133.1"
                     /translation="MVYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDACRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            26185..28887
                     /locus_tag="IWM35_001273"
     CDS             26185..28887
                     /locus_tag="IWM35_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HAP9919134.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            28872..29729
                     /locus_tag="IWM35_001274"
     CDS             28872..29729
                     /locus_tag="IWM35_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP9919135.1"
                     /translation="MYGGIEAGGTKFVCAVSDGGEILEKLSIPTTTPEETLDQVFEFF
                     DRYELKGIGIGSFGPIGIDKTKDNYGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTD
                     VNAAAYGELKQGAAQGKNSCIYLTVGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPD
                     DHYEGTCPYHKDCLEGLAAGPSIEARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLT
                     LAPEKIILGGGVMNQDHLLQKIRQQFVELMGGYMETPQVSEYIVRWGLPNESGIIGSL
                     LLAEHEHQK"
     gene            complement(29800..30657)
                     /locus_tag="IWM35_001275"
     CDS             complement(29800..30657)
                     /locus_tag="IWM35_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAP9919136.1"
                     /translation="MKITITLPMNHPETTIRDLLEKEWLVPRKVRHFLRIRKNVWINQ
                     QPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDEHLIIVDKPAGMKTH
                     PNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKNPFVLPILGRMLEQK
                     MIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSALTHVTVNKVLSEQT
                     QVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHALELHMIHPFTQEKIV
                     GKALPGLWE"
     gene            30868..33039
                     /locus_tag="IWM35_001276"
     CDS             30868..33039
                     /locus_tag="IWM35_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HAP9919137.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPAVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            33107..33448
                     /locus_tag="IWM35_001277"
     CDS             33107..33448
                     /locus_tag="IWM35_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HAP9919138.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(33724..35478)
                     /locus_tag="IWM35_001278"
     CDS             complement(33724..35478)
                     /locus_tag="IWM35_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9919139.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQCISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
     gene            complement(35489..36325)
                     /locus_tag="IWM35_001279"
     CDS             complement(35489..36325)
                     /locus_tag="IWM35_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAP9919140.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            complement(36327..37010)
                     /locus_tag="IWM35_001280"
     CDS             complement(36327..37010)
                     /locus_tag="IWM35_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HAP9919141.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLMPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
BASE COUNT        11959 a         6519 c         7589 g        11010 t
ORIGIN      
        1 tcctgcaagg agctgttttt gagttacaag atgccaatgg aaccgttctg cagtcaggtc
       61 taaccacagg agcagatgga aaactggcga ttgacggctt agcaccagga gtgtatcagc
      121 tggtagaaac ccaagcgcca acggggtatg aattggatgc gacaccaatc gagtttgaga
      181 tcgaaagaag tcagacagcg gtggtagaat tgacgaaaga aaatcgactt acaccaggcg
      241 gtgtcgtgtt gacaaagatc gatgaccaaa gcggagaagt cctgcaagga gctgtttttg
      301 agttacaaga tgccaatgga accgttctgc agtcaggtct aaccacagga gcagatggaa
      361 aactggcgat agatggctta gcaccaggag catatcagct ggtagaaact caagcgccaa
      421 cggggtatga attggatgcg acaccagtaa ctttcaagat cgaaaaagaa caagaagtgg
      481 cgatcttttt aacaaaagag aatagaaaag ttgctgatag ctcagatata agaggaaaca
      541 atccaacaag cagtggaaat aagcatcttc caaaaactgg agaaaccatc attagccagt
      601 ttatattgtc aatattgggt gtgctacttg ttttcatcag tataaaattt agaaagaaac
      661 gaaatatatg agctaaaata aaaaattgaa taagcatagt ttttaaaatc aactagtacc
      721 catcaattga atggaaacta ccgattttag aaggggtata caaacaaata ttagtatcgg
      781 agtagagaag gttgaaccca accagtaaat aagtaaagac aattttgtgc tgcgttgaga
      841 agtgaaaaat aaacctttgt agtaatacta gttaataaaa aatatacaaa aacactgcga
      901 aaatcattct tgagtttcgc agtgtttttt gcctaatgac tcaagtttat tgggtaatac
      961 tttgattttc tttctacaaa taatcagcga tcagttgtaa ttttttctct agttttaatg
     1021 gctctttgtc aataaggaga aaaacaagtc gggtcttttg tctcggaaag cttcaaccaa
     1081 ctcgtgaata aaggttacgc ctccttgaaa ggcgtggaaa aaccaagtaa tcgatcaatc
     1141 accaatttcg catttggtag aacatgtttg gtgagctgaa agtaggtggc gttcatgtct
     1201 gtaactaaga attctatttc tttaggatta gtgcagctaa ggaaatagtt tgttaatcga
     1261 ggaatttttt gtgtaggtaa attttctcga ttaatttttc tgtctcgcca tccgcggaaa
     1321 taaaactcag tttatcctca acggaaacgt gagaacgaaa ttcacaaccc tcaacactca
     1381 agacagaatt cttttggaag actttggtaa atagcttctc aactctttca atgtgcgaat
     1441 aacggttgtc aaagatacct aacaactttt cgaaataaaa aataacgaca ctttttcagt
     1501 cagtaaagaa gtgattttat gtctgacatg atttgaaatc gagtgccttg gacggataaa
     1561 ataactttga gcgttccaat ggcttcgaca gtttttacag gtatagcgct gcttttttag
     1621 gcgtataacc attggcatat gattatatta ttcaaagtgg acaatggttt ccttcttctt
     1681 gtttttcacc accattaatt tcctatttcc atctaccaca gcagaaccac aatttctaca
     1741 gacacatgga gcagggaaga gaactgcagc aacgatcaat gtttttttct tctgtaaggt
     1801 ttcgtaagag acctctgtaa tcatcaaatt tttgtctatt gatctcagaa tttttttgat
     1861 agaaccattc ataaagcata tcgttctctc agttgtttat tttgtggtga tttaatcata
     1921 caagaaaacg atatgttttt ttataaaaac atgaatagga aaaatagttg acaagaaaat
     1981 gacaacgcca tatgatgttc catagtattt acaacctgtc atagatgact gtaaaaaata
     2041 aaagtaaaaa gaaaggacga caacaggtgg atttttatca gtctctacaa ctagatccgt
     2101 ttattttaaa acaaaaaata cgagaagcag cttctaaaaa agaaaagtgc atgtatattt
     2161 gttcattgtt tttacgatca ttattcatcg tattgtttgc catttgtttt atcattataa
     2221 ttacaacatt atttgaatcg aagcataaac catacgctgt ggttttattc tgtatgttga
     2281 tgagtattcg ttttgttgac tttggttata agatttccca ttcgatcata ggtttagcaa
     2341 tcgtcatgtt ctcactatta gtagcaccgt atgtccagct gatcaaatgg tcagttatgg
     2401 gcatgttgat tcattttatt ttactaagca gcatattaat ggcaaccgct agtgatccca
     2461 aaatggggaa tgcttctttg tacggctttt catatctctt tattgtttat tcgttaccta
     2521 aagattcgtt gaacaagaat ttctttacac aaacaagtag cttattattt ttgttttttt
     2581 gttggttttc agttctcctt tacagaaaac atcgagaaaa gaacaaagac aaaagcttgt
     2641 ttaaagagat ttttttgaaa ggcatgtatt cgcaggaaaa aatttggatg ctcatctatg
     2701 catttggcat tagtcttttg atcgttgcag gagagtatgt cccttttcag cgattgatgt
     2761 gggcagggtt tgcattttct tctattgtat ctagctatgg attgatgtcg attggtttca
     2821 aagaacgagc agtagaccga atcattggct cattgattgg atgtgtttta tttattggta
     2881 tcagccaatt tattcctttt aactgggtcg gtatattagg tggtctagct ttaggtgttt
     2941 gttctaccta tagatataaa acagttttta attcttttgg tgctttggca atcactgcca
     3001 gtttattcgg ggttccagga gcagtaacga tccgcatttt tgagaatatc cttggggtat
     3061 gtttaggaat catgtatatt ggggtgactg aaatacttat ccgaaaaata agagaaaaac
     3121 acggtttgaa tcattagatg agatcgaata aaaaataagg aaaaagtgaa gatacacatg
     3181 gatgataaat tgctatcatt ccatgtgtat ctttttacgg taaatcagtg aaacgttcga
     3241 ctttcatttt taatttatac tttttacgga gttcgtttat ttgagccatc atttcggatt
     3301 tgtgatgctt ttcgattgct tcttcatctt tccaacggtc gatcagtaaa agagattctg
     3361 gatcttctag tggaaagtaa tagtcatatt tctcgtttcc ttcttctgca cgaatcgctt
     3421 tgacaatgtt tctttcgacc atttcttctg caaattttct ggcttgtcca tttgtacttg
     3481 tatacaaaat attcactgtg atactcacta aatcccctcc tgaaaaaatt ctatcactag
     3541 tatatcacgg tgccagaaat tattattcaa gatatgatac acattttttc cgcaatgcta
     3601 cggaaatata tattttgaat agaaaacgct tacaagatat gcttggttta taaaaataaa
     3661 aggagtgatt gatttatgaa aaaagcattg attatttgtg cggcaggaat gtcatcctcg
     3721 atgatggcct caaagacaac ggatttcttt aagcaaaaaa gagaagaggt actcgtagat
     3781 gcagtttcag ctacagaagg cgataatatg attaagaata gtgattttga tttatttctg
     3841 atcagcccgc aaacgacgat gtttttggat aaattcaaaa aattaggaga atctgttgga
     3901 aagcctgttg ttagtatccc tttccaagcg tatgtcccaa tccctacagg catccagaag
     3961 ttagcagagg tgatcgaaga gaacatatga tggaatagga gtggatgata atgaaacaga
     4021 aggaaaaatt gctgttgaga tatttgattg atcacaagaa ggagtatgtc accagtcaac
     4081 gtttagcttc tgaattgtta ttatctgatc ggacaatccg taattacttg cagcggatca
     4141 aagagttagt agaaaaaaac ggaggtaaga tcattgctaa accaggttat ggctaccaac
     4201 tgcatatttt acaaagactg acatttgact tgtttttgag tcggcaagaa atagtacctg
     4261 gatatgctag agaaccgcaa ggattttatg agtccgaaga tcgacaaaag tatatattga
     4321 accaattgct tttggaagat gccattcttt ttatggatga tttggcagaa gagctttata
     4381 tcagtcgttc cagtttgaca aaggacatgc aagaaatcaa ggagcgattg attccatatt
     4441 cactaaaaat cgtttctaag catggtcaag ggacatggat cgaaggggaa gaaagaaatc
     4501 gacggcattt tatcatggat actttttttg gaaaccatta tggaaattca ttgaaagaat
     4561 atttagggca aagccgtttt ttcccagaca tcagttttga agagctagtc attatcattc
     4621 tcgatgaaac aagagaagct aagttaaaag tttcggattt tatcatacag aatctgactt
     4681 tacacttggc tttggggatc aaacggctcc gagaagggtt tgaaatcaag gaattaggca
     4741 tcgaacaaga agtttctaag cgagtagagt atcaagtggc gcagcggatc gttcaacgta
     4801 ttgaggtaat cgcaggtgta tcgtttcccg aagaagaaat tgcttatttg acattacatc
     4861 tcatggctaa gtcgaatcac aaagaaaatc aaaatgatca ggaactatca aaggaattag
     4921 cgaaagttct gaaaagattg tctcaggaat ttcatttttc attagtagag gatctccagc
     4981 ttttcaatgg actactcgat catttgtcac cgatgatgat tcgattgagc cgaggtattc
     5041 gcattgagaa cccattgact gaggagataa agaaagaaaa tcccaaagtc tttgacgcag
     5101 taaaaaggca tttcagcaac atgccggctt tgaaaaacta taccatcaat gaagatgaat
     5161 gggcctattt ggctttgcat ctcatggcgg cattggaaaa agaaagagct gcgcataaac
     5221 tacatgcatt gattatctgt gcaacgggtt acggcagtgc ccaactatta aagaatcgcg
     5281 tagtcagtga attcggtaaa aatattacag tggtaagtgt aaaaggctat tatgagatca
     5341 atgaaacaac tttaaaagga atcgacctga ttatttcttc tattgatctt tcgacaatgt
     5401 ttttcaaaat tccagtttta catgtcagta tttttttgaa tgaagatgat gttcgtaaaa
     5461 tacgaaaggt agttggagaa tctataccct atcattcctc agatagaccg ataccagcat
     5521 tttctctaca gcaaaaaaag cagatttaca ccgaacagtt gtctgaaaaa tttttcaaga
     5581 cttatacgta cgaaccgaaa aaggaagaaa ttcttcatga tttgctgcaa aagctgagca
     5641 taaatgaaga agaaggatat ataagagaaa tgcgaaagca gatccagcaa cgtgaaaata
     5701 tgggacaaat catattcagt gatacagtgg cagtcccaca tcccgctgtt ccagttggaa
     5761 tcgaaacaaa ggtagccgtt gcattgattc cttctgggat gcagtgggat caatatcaag
     5821 aaattcattt tgttttttta gtttcgcctt cttatgtcga aaatgaagga attacggtcg
     5881 tgactaaagc aatcgttagg ttagttgacc agctaaaagt acagcaggaa atcttagctg
     5941 aacctatttt tgagaatttc agcagtaaat ttatcaaact gatcgaataa ggaggaaaac
     6001 agatggctga aaaaataaac agtgaaacac tacaagtgac ttcttttgat atcattttcc
     6061 atagcggaaa tgcacggaca ctgatacatg aagcatttcg ctcgatgcgt aaagagatgt
     6121 ttgaagaagt agaagagaag ttgcgacaag caaatgaaga gattcttcat gcgcatcagt
     6181 cacaaactca acttctgaaa gaatatgcca gcgggacaaa aatagaaatg gaaattatca
     6241 tggtccatgc acaagatcat ctgatgacga cgatgacatt attggaagtt gcacaggaaa
     6301 tgaattattt gtatcaaaaa aataaataga aaggaaggat cattgtgcag gatacagtaa
     6361 aaaggaaaat cgaattagaa aaaaagcaac ttagtttgaa aaacatgtat ttcaatcgtt
     6421 atctaatcgt acgttacctg actgcctttt tctttttcat gaacatgcaa tggatgattc
     6481 tgcttctatc agctaaaagt ttaggaagta gtttaccgat gattctgatt ttagctattt
     6541 tgcctgcagt aggagaacaa gtaaagcttt atcgaaaaca ccaaacgaac gttccatgga
     6601 caaagtggta ttttttgttc cagagtgtct ttaatttatt agtgattcca gtgttgttta
     6661 cctcaggatt tacattcttg tatcctttta tggcaaataa caatggagga cagttatttg
     6721 ttttcattct tgttgtaagc ggtatttttg ccagtgcatt tatacagtat cgtttaaaga
     6781 aaatttcact caaccaagac caacagtata tccgtattaa acaatatgaa aaagcgttat
     6841 atttaggaaa ggagaataac taatgtttga ttttttgcag aaatacctga tgggacctat
     6901 ggggaaaatt gcgcaactta aaatcgttcg ggctgtcatg gcagcgggga tggcttctat
     6961 tcctttcacg attgtaggct cgatgttttt ggttttaaat attttgccat taccgttccc
     7021 atttttagaa ggatttttta atgcaacttt tttcaaagtt agtgacttgt acatgatcgt
     7081 caatacaatg acgatgggga ttttatctat ttactttgcc attgtgtttg cttatgaatt
     7141 aacgtcaatt gaacgagatg aacaaaaatt aaatgttaat ccgctcacgg gggcattact
     7201 atctgttttt gcattcttta tgtgtatccc tgaattgatc atgactgatg gtaaaatcag
     7261 cttgattgct tctatcaatg agacagaaac tgtagtaaat ggtattagga tgggctcttt
     7321 tgtcgaacgt ttaggaactt caggaatctt tacagctatt attatgtcga ttattgcggt
     7381 cgaattgtat tgtatgtgtg tcaagagaaa ttgggtcata aaaatgccag atgttgttcc
     7441 gccaggagtt tctcgttctt tcactgctct gatcccaaca tttgtcattg cgtttgtggt
     7501 gatgatcatc aatggattat tagttgcact aggaacggat atcttcaaaa tgattgctat
     7561 cccatttggg tttgttacgc agttgacgaa tacatggctt ggtgtcatgg tgatttattt
     7621 cttgatccat gcattgtgga tcgtagggat ccatggagcg aatatcatca cttctttctt
     7681 gaccccgatc gtattagcta atatggcagc taacgcacaa ggcgcaaatt atccgttggc
     7741 aggagaattc aacaattctt atgtaaccgt tggtggatca ggtgcgactt taggattgat
     7801 catctttatt gcattcctcg cgaaatctga tcagctgaaa gtgttaggaa aagcctcact
     7861 tgtaccagga ttattcaata tcaatgaacc aatcattttc gggatgccaa tcgtctacaa
     7921 tccctattta gcggttccgt ttttcttagc tccgatggct tctgctacac tcgcttattt
     7981 tgccattcgg ctggaaatcg tcaaaccgat gcttgcacag atgccgtggc cttcaccagt
     8041 cgggatcgga gcatttgttg gtagtggtgg tgattggaaa gctgctgtgt tagcagtcat
     8101 ttgtgcaatc atcgcattca ttatctggtt cccatttatc aaattctatg ataataaatt
     8161 attaaatgaa gaaagagaaa aggcagcaga attagctagt gaaaagttaa aatgatttct
     8221 aagaaaaaag gaactgtgac aaaaaatcac agtccctttt tttcttagaa atgggaaacc
     8281 actagtatat aaataccatt attcttcaat caaccgcttc acgattgcat aatccccttc
     8341 ataaggttca tctacaccat tgattttttg atagggatcc acgccttttc ccgcaataac
     8401 gactgaatct tgcggagcgc tttctgctaa agcgcgtcga atcgcttcag gacgatccat
     8461 ttcataatga acgatcaaat ctggattagt aattgcttca ttgatttctt ccgcgatttt
     8521 tttcggatct tcaaatgctg ggtcatctgc cgtcaaaaag gcgatgtcag ctgtttctga
     8581 tagcactttt ccaaaatcat gtcgacggga gattgctttg tttcctgggc tgcctaatac
     8641 aacaattaca cggccatctg gatgttcatt tttggcaaat ttcagcaatg ttttcaagct
     8701 tagataatta tgtgcatagt ccacatagac atgcgctcca ttggattgga tcaattgatc
     8761 cattcggcct ggtacacgag cttcttttaa tccgtcttgt gcatcttgac tggaggcacc
     8821 tgttagtgct gcggcaatga tcgcactggc tgcattttct tggttgaatc ctccagccag
     8881 caaaatatca taagcagtat cgcagatagc taagcgatct tcatgagaag ataaagtaaa
     8941 accgtgagta cctttatctg aacggatcac ttgataattg ctttcttccg aacgaccata
     9001 agtgatcgtt ggaatctgga atagatcggc tgtttcccta agtaaatcaa aatagtcact
     9061 ttcatgattt aaaacaactg tttttgaatt caagatcaat tgtcgcttgc aatagaaata
     9121 gtcatcaaat gtcggatgtt cgatcggact gatatggtca ggcgagatat tcaagaagat
     9181 tccgacatct aaagtcaagc cgtatacgcg ttgtgttttg taagcttgtg aagaaacttc
     9241 catcactaga tgagtcatac cattttcaac tgcttctgac atcatacgat ataaatctaa
     9301 tgactcaggc gttgtcaaat gggatttgaa atatgttttg ccatctaacg ttgtattcat
     9361 agtagaaagc aatgctgttt ttttatttgt tgtgtgatcc aagattgctt ttgtaaagta
     9421 cgcagcagtt gttttacctt ttgttccagt gaatccgatc actttcaatt tattttgcgg
     9481 atagtcgtaa aaagccatag caagaagcgc cattgcttta cggatatccg ttacgatgat
     9541 tcctactgct gaattttcgt atggttgttc tgatacataa tattgaatac cctgttcgat
     9601 tgctgaatcc aaatattctt ctttgaaatt cattccttta cagaaaaaca aagtagaagc
     9661 atcagctttt cttgaatcat aacttaactg aagaaattca gctttttctg gtacatcaag
     9721 atgccagcct cttgttgaaa caaattcttt taatagattt tcttttaata gtaaatgacg
     9781 gatgtcttct aaagagaagc tcattgattg atccctcgct ttattcaatt tcttggttta
     9841 ttatagcaca ctctttttaa tatatcgttt gtttccgcta tgacaaaatc cagctttatt
     9901 caaattttct tgacacaggg gacaagcgga tgagtatttt cataaacaat gaactgactg
     9961 taatagatag gagaacgaat agtgtctcca acaatccaaa aacataataa ttcaaaaaga
    10021 cgataagact attggtaagt aaaagtcctt gcggcattgg aagccgaaaa aatttgtgga
    10081 aaataatcgg cggtatcgtt ataccgccat ttgaggcccc gactgtgtac aatgtgtaaa
    10141 ccccaagact gaacaaaaga cttcctgaaa tcagtgaaaa gaaatgtgag gagatcattt
    10201 ccacttttgg aacaatctct aaaacaacag gaaaaagcag acttcccgct acagtcttaa
    10261 aaaatactga ttttcctaat aggacagcgg ctagaagcag catcataaga ttgatgctcc
    10321 aaacagtaac tgagcgttgg atagaaaagg ccgtttcaac taaatgtccg atggcgccca
    10381 ctccggcgga agctatatcg tgagggagca agaaccaatt caatccaact gtgataaaga
    10441 aaagacctaa aaatataacg ggtagttttt ttatttcttg tgtcattgat atctcctata
    10501 aatgttagcc ataaaaggct gtatatttct ttactttacc aaaaaaacga aaccgaattc
    10561 aaaaaaacaa ttgttcttat aaaagttata taaaatcgat tgccagtttc tgttttacca
    10621 attaaaatga tagtaatgtt ttgtagaaag ttggaatctg accgatggat aatcaaagaa
    10681 aaccagcatt agatcaatta acaccgcaag aaaaaatggc acgaggctct gcttggatga
    10741 ccgccagtaa tatcatttca agattgctag gcgctgtcta tatcattccc tggtatgctt
    10801 ggatgggcga aaatgctaag gctgccaatg gattattcaa tatgggatac aatatatatg
    10861 cattgtttct actgatctct acagcaggaa tacctgctgc gattgcaaaa caaactgcac
    10921 gctataattc attgaatgaa tatggcactt cacgacgatt atttatccgt gcattacaga
    10981 tgatgggcgg gttaggcctg ttgtttgctt tgttcatgta catcgcctct ccttggctag
    11041 ctcatgcttc tggtggcgga gaagaactga ttccaacgat gcgttctttg agtatcgctg
    11101 tattggtttt tccttgtatg agtgtgatac gagggtattt gcaaggcaat caagaaatga
    11161 tgccttatgc gctgtcgcag attgtagaac aggtggcacg tattttttat atgctattgg
    11221 ctacattcat catcatgaaa gtcatggatg gtgactatgt gacggcggtc acgcaatcaa
    11281 ctttcgcggc atttatcggt atgctagcaa gtattcttgt ccttttgtat tttttgaaga
    11341 aacatcaagc gtatacatcg gcgtttattc actacagcga aaacaaaata aatattgcaa
    11401 cgaaggaatt gttgttagat accgttaaag aagcgattcc gtttattatt gtgggttcag
    11461 gggtaacgat cttcaaatta gtcgatcagt tcacttttat gaagatcatg tctgcatcca
    11521 ccgagtattc caatgcacag ttactggatc ttttttcgat ttttagtgcc aatccagata
    11581 agttgacgat ggtagttatt gcattggcaa catcgatcgc gtctactggg ttaccgttga
    11641 ttacagaagc tgttacgttg aaagatcgag taggtctggc aaaattgaca agcggaaatt
    11701 tacagttgtt ttcatttttt atgtttccgg cgacatttgg tgttatgtta cttgcctatc
    11761 cgctaaatac attattttat acaccggatt ctctaggaag taatgtactg atccaagcaa
    11821 gttttgtagg actcttccta ggactatata tgctagtttc caacatgctg caaggaatgt
    11881 ttgaaaacaa ggcggcaatt aattatttgg ttattgggtt tctagtgaaa ctccttcttc
    11941 aatacccagc cattcgttta tttgaagtat acggcccttt attggcgaca atgatcggat
    12001 ttgctgtgtc ttgtaccttg atcttaaaga gaatccatga agtagcccgc tttaaaccaa
    12061 gttttgtctg gcgcaggaca ttgctgatct ttattcttac cttgattatg cttttggctt
    12121 cgtggatcac aaagatgatt tttggtgctt ttttaaacga agatagcaaa ttccagtctc
    12181 tattgttgat tcttgtagta gcaggtatag gggctttagt gtatggttat ttagctttga
    12241 aaatccgctt ggcagaaaga ctactaggac caggaatagc gcgtttacga cataaattaa
    12301 aaataaaata agtgaatcag aagagctaga ttgatgctct tttgaaatgg gggaaacgca
    12361 tgcgtttaga taagttttta gcagatatgg gacttggcag ccgcaaagaa gtcaaaggat
    12421 taatcaaaaa aggaacgatc gaagtcaatg gacaagtggt gaaaagcgat aaagcgcaag
    12481 taaatgaatt cgaagatgcg gttacttatt taggagaaat cattacgtat caaaaagatt
    12541 tttactacat tttgcataaa cctgcaggag tcatttctgc tactcaggat aatcatgatc
    12601 aaaccgtaat cgatttattg gaagaccagg attatcggga agatcttttt ccagtcggcc
    12661 gattagataa agacacagag ggcctcttga ttttaacgaa tgacggggtg tttgcccatc
    12721 agttactatc acctaaaaaa catgtggaga aagaatatct agctgaggtt agcggtataa
    12781 tgacagaaga agatatccgt ttatttgcag aaggactgat tattgatggc gatgagcaga
    12841 cattgccggc aaaactccaa attgatcaag tagatcaaga gacagaaact tcaattatcc
    12901 gtctgatttt gcatgaagga aagtttcacc aagttaagcg aatggtcaaa gcagtaggaa
    12961 aagaagtctt atatttaaaa agaatccgta tgggaaaatt ttggctgcct aaagatttga
    13021 ataaagggca atatcgaccc atgaccaaag aagagctaga acaagtgaga gaaaaataat
    13081 tgaatggata gctaaaagaa taagcagagt agttgtagcg ccatctgaaa agcagacttg
    13141 tccgtctgac tttcggatgg cttttttgtg aaatttcggc ttatttccgt agaagctact
    13201 tctggaacac cgtttgttcg gaataaggga ctgtgacaag acttttgtta cagtccccat
    13261 tgttccttgt gactcagagt tagatagtaa ggacacgatc tccctaatac tcaggccaaa
    13321 acgctgaatt cacaacctct gataaacggt gttcaatcag ctgaccctcg gtattagctc
    13381 aaaaaacggc aaaatatgag gaactatttt cccgtttttt tctctaatac tcaggccaaa
    13441 acgctgattt cacaacctct cctatacttt gtttcatttt ccctatactt tgtttattta
    13501 ttctcattat tgaaagcgct ataattacgt tgcgaatgaa tcgaaagggg tttttagatg
    13561 aaaaaacgaa aggggttcaa tcattttttt atgaatctat ggcgttataa agcacttgtc
    13621 ctgatggctg taccgggtat gctttggatg atttttttct tttacatacc tgtaatggcg
    13681 aatgtcgtag cattcaaaaa ttttcatatt tcagcagatg ggttcttggc tagcttgagg
    13741 gaaagtcctt gggtgggttg ggaaaatttc aaatttttat tttcttcgga tcaagcattt
    13801 ctcatcacta aaaatacaat tttatataat gtagcattta tccttttgaa tctgctgatc
    13861 tcagtcgtat ttgcaatcat tatgagcgag ttgagaaata aacgactcgt taaagtttat
    13921 caaacgatgt ccttactacc ttattttcta tcttgggtca ttatcagtta ctttgtttac
    13981 gcttttttaa gtcctgacaa aggaatattc aatcagtgga tcactggaca tggaggagaa
    14041 gctgtcaatt ggtataatga accgaaatat tggccgttta tcttggtgtt cattggtaca
    14101 tggaaaggga ttggttataa cagcattatt tactttgcat ctgttatggg aatcgatccg
    14161 acttattatg aagctgctat ggttgatgga gctagcaaat ggcagcagat caaacatgtg
    14221 acgataccac aacttattcc actaatgacg attttaacga ttttagccgt aggaaacatt
    14281 ttccgagctg attttggcct gttttataac attcctagga attccggtgc attatatgat
    14341 gtgactcaag ttttggatac gtatatctac aatggcctga catccacagg agattttggt
    14401 atgacggctg ctgcaggact ttaccaatca gttgtcggct ttgtcttgtt gatgatcaca
    14461 aatacgatcg cccgacgttt tgatagcgat tctgcattat tctaaggagg aaaaaaatga
    14521 aaaaaaagaa agtggagaga gtccaagtcc gctctttcaa taagacggct aattttttct
    14581 ctaatataat gattgcactc tttgctattt cctgtatttt gccatttata tttgttatcg
    14641 ccatctcttt taccgatgag tccatattgt cgactcacgg gtacagtttt tggccagaaa
    14701 agttcagtat atttggttat acgtacttgt tcgaccagat gcaggataaa attttccaag
    14761 cgcttttcgt cactgtactt gttactgttt taggtacact gatcaatagc acggctactt
    14821 ctttatatgc ttatgcgatt tcacggtcaa actttccatt ccgtcgtttt tttaccgttt
    14881 tttgtttgat tacgatgttg ttctcaccag ggatggtggc caattatctt gttatgacaa
    14941 atcttcttca gttaaaagat acgatttggg cattgatctt gccaatggct gtcagtccct
    15001 tcaatattat tgtgatgcgc acattcttta aacgttcggt ctctgattcg attattgaat
    15061 cggcacggat cgatggagct agcgaactgc gaatatttgt tcagatcgtt ttaccgttag
    15121 ctgttccagg tattgcgaca atcagtttat ttgccgcatt gggttactgg aatgactggt
    15181 tcaatgcttt actatacatc caagatgata agctagttcc actacagtat cttttgatga
    15241 aaatccaaag taatattcaa tatctgacac aaaattcagg agcaggaagc caattgacag
    15301 gcggattagc ctcagttcca ggagaatctg ccagaatggc gatcgtagtc atctcgacgt
    15361 tgcctattgc gatcagctat ccatttttcc agaagcattt tgtcaaaggt ctaacaatcg
    15421 gcggagtaaa agaataaaga aacaaaaata taaatcataa aattaggagg aagaaaaatg
    15481 aagttatgga agaaactagc ctttacaggt gtttcagcgt tgttgttggg aacattagca
    15541 gcatgcggag gttctggttc taaagaccag gcagaagcta gcaattcgga cactttgcag
    15601 atgtatcaaa tcggagataa accagataat ttcgatcaat tgatggaagt agcaaacaaa
    15661 cgaatcgaaa aagagatcgg tgtaaaagtc aatatcaatt acattggctg gggcgattac
    15721 gaaaagaaaa tgaacgtcat catttcttcc ggggaaaatt atgatatcgc ctttgcgaac
    15781 aactatgttt ccaatgccca aaaaggagcc tttgctgatt tgactgaatt agcgcctaag
    15841 tacgctgaaa aagcttataa tgatttagat gaagcatata ttaaaggaaa tctggtcaat
    15901 ggaaaattat atgctttccc agtcaatgga aatgtctttg ctcaacaagt acttactttt
    15961 aacaaagctt tactagataa atacgatctt tcaattgatg gaatccaatc ttacgctgat
    16021 gcagaaaaag tcttgaagga attccatgaa aaagaaccaa acacggcagc ttttgccatt
    16081 ggacaaggct tcaaagtcca agggaacttt gattatcctt tagggaatac gttaccattt
    16141 gctattgatc tggatggaga tgattcgaaa atcatcaacc aatacgataa tgaaacgtat
    16201 atcggactgt tacgtacaat gcatagctgg tacaaacaag gactcattcc aagtgacgct
    16261 gcaacgagca ataaggatta tccactagaa ggaaatactt ggctaatgcg tggagaaaca
    16321 caaggaccgt atgattatgg cgatacaatt ttgacaaatg cagcaaaaca agaacttgtc
    16381 tcaaaagcaa ttactgttcc actaaaatct acagctcaag cacaaatggc aaattttgtc
    16441 gtatcaaata cttcaaaaaa taaagaaaaa tcagtagaac ttctagggct gcttaacagt
    16501 gatccagaat tattgaatgg gcttgtttgg gggatcgagg gagaagcatg ggaaaaagtc
    16561 gatggttcag atgataaaat caaattgcta gatggttatc agccaaatac acatatgtct
    16621 gcttggaata caggaaacaa caagattcta tatacacaag agtctattac ggatgacatg
    16681 attgccaaac gtgatcaatc gattgctgat gcagaaactt caccgatttt aggattcagc
    16741 ttcaacactg acagtgtcaa aacagaatta agtaatattt ctaatgtcat gaatcaatat
    16801 ttagatggat tgaatacagg gactgttgat ccagatgaaa cattaccgaa attgaaagac
    16861 gctttggaca aggctggcta tgataaggta ttgaaagaaa tgcaaaaaca atatgatgag
    16921 ttccgacaag aataataatg aataaagaca aaggggttgt gacaaaagac gtgtcacaac
    16981 ctcttgttcc gaataaacgg tgttcaaaag ccagtttctc ggtattaagc taaatatatg
    17041 taaaaatgtg gaaagccata ttcacaaaat ttttcttact actcgaaact aagcggctat
    17101 ttcacaaact cttggtaaac ggtgttcaat cagctaaccc tcggtattcg ctcacaaaac
    17161 gacaaaatat gaggaactat ttttccgttt gtttctctaa taatcaggtc aaggtgctga
    17221 ttgcccaacc tttttttgta taatgagaaa gtataaactt aatgtttgga ggagaagata
    17281 tgttagggaa ggttttggaa gagttattta tccggatctg ggtcattata aaactaacct
    17341 tgtatttctg gatatataca tttgccggcg ggatcatctt tggattaggt gcggcatgga
    17401 aaacagtcaa tgaactattt tatttgtacg gattcgagta taaagaaatc acaataaaaa
    17461 gaggctggaa tatatataaa agaaattttt taagaggcaa tctgctattt agtttatttt
    17521 tatctggtac tgctttattg agttacaatt tatatttatc tgttcaaatc aaagggctca
    17581 tttttttagc tattgatttt attttacttt ttgccttgct ctgcctattt gctacttatc
    17641 aatacagtat gattttagat agtgaatata cgatctctgt tcctaatctg ctaaagctaa
    17701 gttttatttc ggttttttcc agcttttcgt cttttctaaa aataattatt ggtagcggta
    17761 taattttagg cgtgacttgg caattcaaag gattgatcct gtttggtgtc attggtcttt
    17821 tgacggtttg gaatggtacg atgacaacac attggcgtga agaattagat aaacagttgg
    17881 aaagttatga ataagtttcg gattctattt tcaaaaggaa ccttactaaa tcgtctgttg
    17941 cggaaatacg cggttatcat gatcggtgta acgatgactg ctaccgttat ttttagtatc
    18001 catacttggg aacaaaatca aaaacaggca gaaaacatga caagtgatgc cgttcaaagc
    18061 acaagcagaa tgctgaatga taaaacaacc ttgagtagga tcatcaaaaa ccagcttgta
    18121 gggaattccg aaaagatcga aaatgtgacg acctacttaa caaaacccat agatcagtat
    18181 ctgatgtatg tatacgaaca gcaaaacagt accgatgaat tagtttcttt cccaaatcaa
    18241 atcaaagatc tctacgcaaa ttatgaagaa ctaagtgcga tatatatcgt gttgaatcaa
    18301 ctaccggagt attatgaatc ttctagagaa aacaaaggtg gggaaataaa aaaggggaca
    18361 ccaaaattac tagacgcctt ttatatcaag ttgcctatca cacaaggagg agctgagtca
    18421 ggaagtattt ttattggttt tcaaaagaaa gaactagata tgattttaga aaatctaacg
    18481 tcattcaatg gactctcttt atacatgatt tctggaacga caaatcgact atacacattc
    18541 catgataata aaatagctaa agaaacgttt gaaagacaag aaatgatcat cacagagagt
    18601 ctgaagaaaa attcacaatt gccgattgac gatctggaaa aaaacaattt tgttcaacac
    18661 caagaactgt ctgatgatta tcgtatatta gctgtactag ataaaaaaag tgtaaaaatc
    18721 gaaacgggaa aaaatcttgc cccgctgatc attggtatca ttattttaga caatatactg
    18781 cttatctttc tttataaaac attcaaacga tattcacagc aagtatctat cgtcatgcag
    18841 gccatggatc aaacagcagc agggaattta gagacgcgga tcgacataac aaacacggaa
    18901 tatgaactga gggaactttc aatcggtatc aacgaaatgt tggatagcat caatcagttt
    18961 gtagaagaca tctacaaatt agaaatcaaa cagcaagacg cacatatgcg ggcgcttcaa
    19021 gcacagatca atccacactt tttatataat acattagaat atatccgaat gtatgcaatc
    19081 agcgaaggaa gtgaagaatt agcagatgtt gtgtatgcgt tttccgcttt gcttcgaaac
    19141 aatacgaatc aagagaaaac gatcacttta aaagaagaac tggacttttg cgaaaaatat
    19201 gtctatttgt atcaaatgcg ttatcctaat cgagtagcct atcattttat gatcgatcct
    19261 gatttggaaa agatcgaagt acctaaattt gtgatccagc cattagttga aaattatttt
    19321 aaacacggta ttgattttac tcgattcgat aatgcgctaa gtgtcaaagt cttgcaggaa
    19381 ggcaaacggg taaggatcat catcaaagat aatggaaaag ggatgactga aaaacggcta
    19441 aaacagattg aagaaaaact gtctcatcct aaagtggaac ttcacggttc tatcggtctg
    19501 caaaacgtga atgaacgttt acgtgccagc ttcggcagca gttattatat gtcgcttgag
    19561 aataatgaga caggtggact gaccgtttct attacattca aggagggatg agcgtgtatc
    19621 aagtgttatt ggtagatgat gaatacatga ttttgaatgg gttaaaaaag ataattgatt
    19681 ggcaaagttt aggctttcaa atagtagcaa cagcagagaa tgccaaagaa ggactagctg
    19741 tacttgaaca gcgacagatt gatttagtag taacggatgt gacaatgcca gaaataaatg
    19801 gactggaatt tattgaagcg gctcaaaaag aacgtcataa ttttgaattt atgattttat
    19861 caggatacca agaattcgat tatttgaaag gaggtatgca attaggcgcc gtgaattatt
    19921 tgatgaaacc cgtgaataag ttcgaattgg ttgaaagcct caagaaaatc aaaacacggt
    19981 tagatcagca aaatgaacaa aaaaatcaac aagaaatcta tcaagaactg ttattcagtc
    20041 aatggttgaa tgaagaattg gatgaaataa gtgaagaaga aataatagga caaataagtg
    20101 agaagcagca taggatagta ttgattcaac tggcaagaac tcatgatact cttatcaata
    20161 gatggttgca agaacggcaa caaatctttt actatcaacg aaattacgga gaccgtgtat
    20221 tgatcacgtt gttgttggag gcagatgaag ttgatggctt ctgttgtttt gttcaagaaa
    20281 actttcctga tcaggaatgg ctgatcagta tcggggaaga gacagattct ttggacaaaa
    20341 tccctgaaac attccaacaa gcgaaagaca atctacagtt gcatcaattt tatggagata
    20401 aagacgagca tgttttttac gcagtacaag cagaaacaaa agatcaaacc atcgattttt
    20461 caagtttcag acgcgtgctg caaaataaac aattggaagt cgctcaaaaa atgatcactg
    20521 atttttttga acagtttcag ttagcagcca tgatgccgga agatatacga tattccagtt
    20581 ttttactgtt tatggagatt caacgggaat tgattgatct ggaagatgag gaatatttac
    20641 aaggaatcga aaaaatccat caggcaaaaa cggtgcaaga acttcagcag ctacttctgt
    20701 cattcatcaa aaagcatcag agacagaaaa agtacagtga caatgtggaa aaagtaatcg
    20761 taatcctgca ccagcattat caagaaccac ttacactcaa agaagtgtca gaaaacctgc
    20821 atttaaatgt catgtatcta ggacagctgt tcaaaaaaga aacgaaaaaa agtttttcag
    20881 cttatttgaa ccatttacgt atggaaaaag cgaaacaact gcttttgcac agtaatcaaa
    20941 acatcaatga gatagcaagt gagattggct acaacaacac cacgtatttc tctaaattat
    21001 tcaagaaaat cgtcggcaga tcccctaaag agtaccgaga aaacagtgga gaatcataaa
    21061 agaaaaagcc aatgtaatga aataacttat aaaaaattgt ctagaaatct aattttgggc
    21121 tgttcttatt acttgaggca aggcagcttt ttcatgacct ctcgataaac ggagttcaaa
    21181 agccagtttc tcggtattaa gccaaattta tgcaaaaatg tggaaagcca ttttcacaaa
    21241 atttttctta atactcgaaa ctaaacagct ttttcacgac ctctccacta aacggtgttc
    21301 aatcagctga ccctcggtat tagctcaaaa aatggcaaaa tatgaggaac tattttgcca
    21361 tttgtctctc taatactcag gtcagaacgc tgatttcaca acctctcaat aaacggtgtt
    21421 caaaagcttg catctgcggt aataagacca accaaacgaa aaatatgagg atctattttt
    21481 gcttggctgt tcttattact tgatgcagga cagctttttc acaacctctt gttaattata
    21541 gtttgataag gaaaaaggca aatcttttgc ttgatatttc ttagggtgag ggatgatccc
    21601 taattggtaa gagatcgtgc cgcctttcag caaatcttga tgggtaaaat ataatttcgt
    21661 atattctctg tccatccgtt tgattttatg gataaattgt tgctgcggcc gattaggcgt
    21721 tgcttcaatc gtcaattcct ttccatttga caaaagcaaa acagccttgt caattaatgg
    21781 cataccgata acatattcac cggttccagg cgtgacagga taaaatccca gactattgaa
    21841 gatataccaa gcggccatac ttccattgtc ctcatctcca gggtaaccca ttggagaatc
    21901 gtcgaaggtc tgagtcatca actgtttaag cagtggctgt gccatttcag gttgtcctat
    21961 ataactgaac aagtaaggat agtgaaaact tggctggttt gaaatagcta attgtccaaa
    22021 ttctatcgcg gccatctcac tcatttcgtg gatctcaaaa ccatatcctc ctgggtcaaa
    22081 cgttgggggc tgattgcaca gctcaatgag tttttcttta tatctctcac tgccaccata
    22141 ttttccgatc agtcctgaaa aatcgtgata aacagcaaag ctgctttgcc aagcacttcc
    22201 ttctgcataa tcttgtcccc aacgattagc tgaaaatggt tctctgaact tgccatcttt
    22261 atcttttgaa cgcataaatc ctgactcata gtcaaagata ttttgatagt tcaatgattg
    22321 ctgctgatat ttcttcgtca tttcctcttc ttctagtacc ttggcaactt ggctaataca
    22381 aaaatcgcta taagcatagt ctaatgtgtg attgactgat tcgtggtagt gattaggaac
    22441 atagccatat gtcaagtaat cttctgttcc ttggcgtcca taatttggat gtttgctttg
    22501 aaccgtagca cctttttcca ttgccgttag aaattcaggc atcaagtctc gtcggatatc
    22561 ttttacagca gcatctgcaa tgaccgcatc gatcaatgtc ccaggcataa gtccacgttc
    22621 atcaggagac agccattttg gcaaatagcc actttctcta tagctgttca aaaaaccttc
    22681 cagcatttct tcgtattttt ctacagcaat caaagaataa agaggataaa ccgttttata
    22741 cgtatcccaa aacccattat ttgtatagag aacaccaggt tttatagatc gactagtcgt
    22801 atcatagtgg attttttgcc cactaggatc aatttcataa aatgtttgtg gaaataaaaa
    22861 cacccgatat aaattatgat aaaacgtccg caattgttgt tgatcatggt gtgtgagctg
    22921 tatacgatca aaataggact gccattgttc ttgactgtga gcaaggtaag tctctttgct
    22981 ttggttttgt tctagttcca ggttataagc tgcttgttcc gagctgataa aagatgtccc
    23041 aaatcggata gtttgttttt ttgtctgttc aaaatacaac gggatcgagt cgctattttt
    23101 gttggaaaca gctaactcat caattgttaa aggatattca aatctcaata taaaataaaa
    23161 agaaaaatca ggatcttctg aaccagcaaa atgtgtgatt tttccttcca ctgtgtaagg
    23221 atctataaca aagagctgat ggcgtcccgg aaaggataaa agtaaaccgc tgtcattttt
    23281 gctgtaatca atagataaga ttccgccata catgctaggt atcaatgtag aacagatgcc
    23341 ataacgtaat tgacagatcg acagatgagt cgggcaaaat atactttctt ctgggcggta
    23401 agaactttga gcatggaaaa gggtgttctc ctgtagtttt ccattgatcg gcgtaaacag
    23461 catatgactg aaatcaccca tccaaggact tggttgatgg gtaagacggt agccttgaaa
    23521 aacgcggtca tcaggatgaa accaccagct gccgttttga tccgtagttt gcggtgcaaa
    23581 ataattcatc ccaaaaggaa cgccagtata aggctggcag ttcccattag agaaatttgg
    23641 ctgattcgct gttgcatgtc tagtatctat ttgaccgata ttcattttat ttctcctttg
    23701 ttgattgtct tttgatgaat gaaactggaa atttactatc ggatggtcga tcttttgtct
    23761 ctaaccaagt gaccaaagca tctcccgcaa gcttacctaa ctgttcaaaa tcttgtgcga
    23821 tggttgtcag tggcgggtca acaagagctg ctgcttggat atcatcaaat ccgataattg
    23881 aaaaatcttc agggatatga taaccatgct gtctcaagag attcatggtc tggatagcga
    23941 caacatcgtt ttcacaaaca aaagcagtta cttcatactt agtgatataa gaaagcaatt
    24001 catctaatgt cgcgcttttg tcatccagtg aagtgtggaa agtcaaacca gctttgtcca
    24061 atgcttcaag gtaaccaagg taacgctgtc gaacggattg tggatgagtc tgcgtaccaa
    24121 acaaataagc gatttttgaa tgtccctgtt cgatcaaata agaggcagca agcgcaccgc
    24181 cttggaaatt atcggaaaga acggtaggaa aaggtaactc atagattttt ttatctaata
    24241 tgacgacagg gaattctttc aatgaaagct caaaaagtaa gtcaagatgt tgttctgtat
    24301 caagagcata ataaatcaat ccgtcaaatt cattgattag ctcttttgct gttttatttt
    24361 gtagaaagtc aagcgtcgtc atcatgacat caatctgttt atcttgcatc acagggttca
    24421 atcccttgct aaaatcacca acagacaaat cagcgacata tggaagaaga aacagaatcc
    24481 gtcgtgtttt tgtaggagaa gcaagaatct gttccggaga agcggattta acgaaactgc
    24541 ctttcccgcg aacgcgataa ataagtccta attgctctaa ttcagtcaat gcacgtttgg
    24601 aagtgattct gcttacttta taggtacgtg aaagttcttt ttcagttggt agttggctgt
    24661 ctacaggtaa ttcaccagtt aaaatagccg tttgcaaatc attaaatatt gtctggtaca
    24721 atggttgagt catataaaca cctcttaatg atatatcata ttcaaataat accataatgg
    24781 atgaaaaagt aaataaaaag atattttttg aaagcgtaga cataattggc tgtatgaagt
    24841 ataatcttta aagatatatc aaatagaaag aaggttttat tagtggttta taaagatata
    24901 ccaaaatcgg tgcagaaatt tatggaagat atcacacaaa aatgtgggga tatccatgcg
    24961 gactgggcaa agaattttac agcagcattt gccaatactc tgttgacaac agtcaaaaag
    25021 caaaatgacg gaaccacctt tttattgacg ggagacattc cagcgatgtg gttaagagac
    25081 tctactgcac aagttcgtcc ttatttagtg attgcaaaag aagatgagga attagctgac
    25141 atgatcagcg ggttagtcaa aaagcaattt ttttatatca atatcgatcc atatgcgaat
    25201 gccttcaacg aagaagctaa cggtgctggc caccaaacag atcatacaaa aatgaacgat
    25261 tggatctggg aaagaaaata tgaaattgat tctttatgct acccaataca gttggcctat
    25321 cttctttaca aaaatacagg aaaaactgat caattcaatc aatcttttat tgaaggtatc
    25381 aaaaaaatct tggacgtgtt caaaacagag caagatcatg aacactctcc ttatacattt
    25441 gtacgagaca cgacaagaaa agaagatacg ctgatcaatg acgggcgagg tgcaaaaaca
    25501 gcttttacag gaatgacatg gtctggtttc cgtcctagcg atgatgcttg ccgttatgga
    25561 tatctggtac catccaatat gtttgcggtg gtcgtgttag gttatgtgga agaaattttt
    25621 caaacgatac tcgatgataa agaaattgta cagcaaacac agaaattgaa gaaagagatc
    25681 caagagggga ttcagaatca cggacgaacg gttaatcaaa aagggcaaac gatttatgca
    25741 tatgaagtag atggaatggg aaacgccaca gtcatggatg acagtaatgt gcccaatctg
    25801 atcgccgcac cttacttggg atattgttct gaacaagagg aagattatct gcaaacaaga
    25861 agaacattgt tgagtaaaga aaatccatat ttctatgaag gaaagtacgc aaaaggaatt
    25921 ggatcctccc atacgccaga aaaatacgtt tggccgattg cattagctat ggaaggaatg
    25981 acaacgtcag acaaatctga aaaagagcgg atattgaatt tacttgtagc aactgacgca
    26041 ggaacacacc ttatgcacga aggattcgat gtgaacaatc ctgaaaatta tacgagagaa
    26101 tggttttctt gggcaaatat gatgttttgc gaattagtca tggattactt tgatattaga
    26161 gtagaaaagt aggaggtatc gtaaatgaaa aagaaagtat atatcatttc tcatagccac
    26221 tgggatcgtg aatggtacat ggcttacgaa cagcatcaca tgcgactgat tgaattgata
    26281 gacgatctac tagagctatt tgaagttgat ccgtccttca acagttttca tttggatggg
    26341 caaacgatta ttttagatga ctatcttcaa gtaaggccag aaaaaagaca ggcaatccaa
    26401 caagcaatca atgaaggaaa attgagaatt ggaccatttt atatcttgca agatgatttt
    26461 cttatcagct cggaatcgaa cgtgagaaat atgctgattg gcatggaaga aagccgtaaa
    26521 tggggaaccc ctgtcatgtt aggctatttt cccgatacat ttggaaatat gggacagacg
    26581 cctcaattaa tgaaacaagc tggtatttca gcagcagcgt ttggccgtgg tgtaaaacca
    26641 attggctttg ataatcaagt tttggaagca gaaaactatt cttcccaata ctcagagatg
    26701 tggtggaaag gccctgatca aacagcaatc tttggtctgc tatttgccaa ttggtatagc
    26761 aacgggaatg agattccagt agaaaaagaa gcagctcttg ccttttggaa gcaaaaatta
    26821 gcggatgcag aacaatatgc ctctacgaac cacttattga tgatgaatgg tgttgatcat
    26881 caaccggtac aaaaagatat atcaaaagca atccatctcg caaatgaact gtttccagat
    26941 tacgagttta ttcattctaa ttttactgac tatctggaag cagttcaaaa agatgtgcca
    27001 gaagacttag gctcagtaga aggagaattg acaagtcaag aaacggatgg ctggtatact
    27061 ttagccaata cagcatctgc tcgggtttac ttaaaacaat ggaataccaa ggtccagcga
    27121 cagctagaaa atattacaga accacttgcg acgatggctt atgaagttag tggaaactat
    27181 ccccatgatc aattagatta tgcttggaag acattaatgc aaaatcatcc tcacgacagt
    27241 atttgcggtt gttcagtcga ttctgtccat cgggaaatga tacctcgttt tgaaaaagca
    27301 gatgaagttg gaaagtatct tgcacaagat tccttagaac agctgaccgc agcaatcgat
    27361 acgaccggat tcccaaaaga cagctttcca tttgtcattg taaatactgc aggaatggat
    27421 aaaactggtg aagcagaaat aaccatcgaa ttagaaagaa aaagattcgc tgaaggaata
    27481 cctgaacaac tatatcaaga gttagaaaat ttgcctaaac gtaagtatca tgtagaaaca
    27541 aaaagtgggg ccactatacc agctatttta tctgaagaaa ctgtccaatt tggttatgat
    27601 ttgccgaaag accgtttccg tgtgccatat atggcaagaa tgatcaaagt gactttacca
    27661 ttagaaaaca tgccagcatt ttcttgggaa acattcgcgc ttgtagaagg ggaagcgaaa
    27721 gcagaagaaa aggaaacaat gattcatcag agtggacgaa tcatcgaaaa cggccctcta
    27781 catttgacga ttgaaaaaaa tggtaccatt acaatggaag atcgcaaaaa caaacgaaag
    27841 ttgaataatc tgcatatttt tgaagatatc ggcgatatag gaaatgagta tatctttaaa
    27901 caacctttct gtgatcagcc aatcctatcc agtaacaaag aaaactctga agtaaaagta
    27961 ttagtagata caccggaaat agcaaaaata agtattctac aggaaatgga aatccctgtt
    28021 tctgcagacg agcgtttaga aaaagaacag cagatggttg tggaatttcg ttatcgaaaa
    28081 gcagaacgat ccaaagaaaa acgaatcctt caaataaaaa caatcatgac aatcagaaaa
    28141 gatagtaaaa aaatcgactt tgaaacgact ctcgacaatc agatgaaaga tcatcgctta
    28201 cgtgtcttgt ttcctacaaa gttacatgtg gaacatcacg aagcagatag tatttttgaa
    28261 gtggtgaagc gcccaaacca tgtgtctaaa agttgggaaa accccacgaa tccgcaacat
    28321 cagcaagcat ttgtcaatat tcatgatgaa gagtacggtg taacagtcgg aaactttggt
    28381 ttgaatgaat atgaagtaac agaagacgga caaatcgcag tgacgctact tcgcagtgtg
    28441 ggagaactag gcgattgggg atattttcca acaccagaag cacagtgtct aggtgagcat
    28501 cggttcaatt acagcattga actacatgga ccagaagaaa aattctctac ttatttgcat
    28561 gcatacgcag cacagattcc tttttccact caacaaatca aacatcatga aggaacattg
    28621 atttcaaaac aacaatattt aacgataaaa agtgaaacgt ttgcaattac agctttgaaa
    28681 cgcagcaagt ttagtgataa agtagtagta agaggtttca atatgtctag tcatttagaa
    28741 aaattggaaa taactaaaga caatggaaaa actgtcatat tgaatctgct tgaagaacca
    28801 acgaagcaag cggttgtccc aataatccaa ccttatgaga ttcggacaat cggattcgag
    28861 gaggaaaacg aatgtatggc ggaatagaag caggcggaac aaaatttgtg tgtgcggttt
    28921 cagatggtgg agaaatttta gaaaaattaa gtatccctac cacaacacca gaagaaacat
    28981 tggatcaggt atttgaattt tttgatcgtt atgaattgaa gggaatcggg attggctcat
    29041 tcggcccgat tggtatcgac aaaacaaaag ataattacgg atacgttttg gcaactccca
    29101 aaaaagggtg ggcacaattc gattttcttg gcacaatcaa ggaacgctac aatattcctg
    29161 ctgcttggac aacggatgtc aatgctgctg catatgggga attgaagcaa ggagcagcac
    29221 aaggaaaaaa tagttgtatt taccttaccg tgggaacagg aatcggtgca ggtgtcgtga
    29281 tcaatgaaga aattttttca gggatcgcac atcctgaaat gggacatatt tgggtgaaac
    29341 gccatccaga tgaccattat gaaggtactt gtccttatca taaagattgt ttagaaggat
    29401 tagcagcagg accgtctatt gaggcacgca cagggatcaa agggcaagat ctgccgcaag
    29461 atcatcctgt ttgggctatt caggcttatt atattgctca agcactgatc aactatacgt
    29521 tgacgttagc acctgaaaag attattttag gcggtggggt aatgaatcag gatcatctcc
    29581 ttcaaaaaat ccgtcagcaa tttgttgaat tgatgggtgg ttatatggaa acacctcaag
    29641 taagtgaata tatcgttcgt tggggactgc cgaatgaaag tgggattatc ggtagtttgc
    29701 tgttagcaga acacgaacat caaaagtaag cagacaaatt acacaaaaag aacagagtct
    29761 tatcattatc tgctgataag actctgttct tttttatttc tattcccata atccaggtaa
    29821 agctttaccg actatttttt cttgagtaaa tggatggatc atgtgcagtt ctaatgcatg
    29881 gagcatcagt cgttcagctg ttctattttg gtagagaggg tctccaacga tcggatgtcc
    29941 cttatgagct aaatgaactc tgatctggtg tgtacgtccg gtttccaaaa cacaatagac
    30001 ttgtgtttgt tcagaaagta ctttgttgac agtgacatgt gtcaaggcgc tctgaccatt
    30061 tttatcatct acgacccttt ttctccgatc atggcgatct cgtccaattt tattggtgat
    30121 ggtcatatct ttggcaattt ctccccatac gatggcttga tatctccgat agatcatttt
    30181 ttgttccaac attcttccaa gtatcggcaa gacaaatgga ttttttgcaa ataaaacggc
    30241 accgcttgtt tctttgtcca agcggtggac aacgtaaggt cgttgctgtt tagtgtcgag
    30301 ataagcagct aaatgattga gtaaggtact gttttctgtc ggttcatttg gatgagtctt
    30361 cattcctgca ggcttatcca ctataatcaa atgctcatct tcatataaaa cacggatgtg
    30421 cttcgcatct cctaattgaa cttcttgata attataatct gtttcttcga ataacaatgt
    30481 gatctggtca ttgcccttta cttcatgatg gaacaaagca ggttgttgat tgatccaaac
    30541 gtttttacgt atacgcaaaa agtgtcgaac ctttctagga actaaccatt ccttttcaag
    30601 caagtcgcga attgttgttt caggatggtt cataggtaaa gtgatcgtga ttttcacaag
    30661 tgtactcctt tcagtgcaat tgcagactaa ttgtaccatt tttaaaacat atttaagaaa
    30721 aaacttacat ttttctgttc attcttatgt tactctactt gcactaacgt aaaaaaacat
    30781 attgaactac aacttaagca ggaagaaaac actctttcac tatggtaaaa taagacgatt
    30841 gaatcaaata gacaggagaa gaagcaaatg gattttcaaa gcattttaga aaagataaaa
    30901 gctgctttga tcagtttttg gcgttggatc agaccatatc tcggacaatt ccatcgatgg
    30961 cgaaagcgca tctggaaaaa atatcatgtg aacaaattga ttttgctgat tggtttgatc
    31021 tgtgtcttag tgacaagtat ctacctcttt attttagcaa aacaagcaaa tgtcgaaacg
    31081 ttaaaatcag gattaagcca atcgacagtc atttatgata agaaaaacga agaagctggc
    31141 acactgtacg cccaaaaagg aacatatgta gaattaaacg cgatttctcc ctatatccaa
    31201 gatgcagtaa tctcgacaga agataggaat ttttacgagc atcacgggtt tgatatcaaa
    31261 gggattgccc gtgcagcagt ccgaatggtc ttgtctggcg gaacatcagg gggcggagga
    31321 agtacgatca ctcagcagct tgccaaaaat gcgtatctga cattagacca aacgtttgat
    31381 cgaaaagcga aggaattatt tttggcaatc gagatcgaaa agaagtacag caaagaagaa
    31441 atcttgacca tgtatcttaa caatgcctat tttggcaacg gggtatgggg tgtccaagac
    31501 gcttccagaa agtattttgg agtagatgca acgaatgtta cgctgtctga ggctgctact
    31561 ttggcaggaa tgctgaaagg tcctggtatt tataatccga tcgatcaccc aaaaaatgcg
    31621 aatgatcgga aaaacacagt actaagtgta atggaagaaa acggaaaaat cacaaaagaa
    31681 caagaacaag cagaaagcca gacagatatc agcgcgtatc ttaatgacac atacgataat
    31741 tcagattctg gttatcgtta tccttattat tttgatgcag tgatcgatga agcagttaac
    31801 gagtatggtc ttgatgaaga agatgtcatg aataaaggct ataaaatcta tacagcttta
    31861 gatcaggatt atcaaaagca attagaagct acttatcaaa acagtgcatt attcccagca
    31921 aatgcttcag atggtgccat ggtccaatct gcttcagtag ctttagatcc gaatacaggt
    31981 ggtgtacaag cactagtagg acgaagagga gatcatgtct ttagaggatt cagctttgct
    32041 acacagatgc gtcgttcacc aggatcaacg atcaaaccat taagcgtata tacaccagca
    32101 ctggaagctg gatacaagcc ttccagtatc ttgaaagacg aaccgcaaag ttattacgat
    32161 gcgcataatt ttgacgggac ataccaagga gaagtaccga tgtatcaagc tgttgcacaa
    32221 agtttgaatt tgcctgctgt atggcttttg catgagatcg gtttacaaaa aggatatgat
    32281 aaagcagaag aattcggcct gcctttagca aaatctgata agtattatgg cttagccctt
    32341 ggtggactgg aaaaaggagt atctccattg atcatggcgg gtgcttacag tgcatttgcc
    32401 aatgatggtc agatgtatac gccgcatctt atcacgaaaa tcgttgattc gactggagcg
    32461 gtgatcgtcg ataacacaaa caaaaaacca aaacaggtca tatcaaagga aacagccgat
    32521 gaaatgacca gcatgcttct tggtacattt tcaaatggaa cagcggctgc tgcgaatcct
    32581 gcaggttata cgatagcagg gaaaacagga accacagaaa ctaatttcga tgccacaaaa
    32641 gcaaatgacc aatggatgat cggttacacg ccagacgtag tgatttcgac atggatgggt
    32701 tacgaagaat ccagcaagct gcactatttg gaagggacaa gtggaaatgt tgtcgggaaa
    32761 gtcttcaaat cagcggcaga aggcatcctt ccgtatactt caaaaacagg atttagtgta
    32821 gcagatgcct atgccacagg aggacaagtg gtagctgcag atcaagttgg aaacacagga
    32881 aattctaatg gagagtctgg aaattggcaa gataacttga atcaatatgg tgaacaagca
    32941 aaagaagggc tgaaaaactt cggcgatatg gtgaaagaag gcgttcaggg aattggtgaa
    33001 gcggctaaag acctctggcg gaaatatcaa ggagagtagc ccctttgaaa acgaataacc
    33061 ccatgttaca atagagagta actactaaaa gaaaagaggg cataacatgt caaacatcta
    33121 cgatagcgcg aatcaaatcg aacgtgagat ccgtgaatta ccagaattca aagcgttgaa
    33181 agaagcgtac gagaaagtac aagcaaacga agaagcacac aaactgttca aagacttcca
    33241 agcgatgcaa atcgaattac aagaaaaaca aatgagtgga caagagttca gcgacgaaga
    33301 tgcaatgaaa gcacaagaaa tggctgtgaa aatccaatca gaagaagtaa tcaatgactt
    33361 gatgcaaaaa gagcaaggct tcagtacaat catcaatgac ttgaaccgta tcatcatgac
    33421 accagttcgc gacctttaca gcgaataaaa ctattttatg aaacaacaga gggtgtgaaa
    33481 gagacgttcg gccttgagcg atgacaaaat tgcttcttat tgccgatagg ctgtctcttg
    33541 aacaccattt accaagaggt cgtgagccta ccgtttaact ctgagttaca aggaacaatt
    33601 tcaaaaaaca gcttctcatg tttgatgact attgtgactt gtgaccgagg agttggcagg
    33661 agaacaccgt ttattcagaa ataggagcta cgacaaaagt cttgtcatag ctcctatttt
    33721 ttgtcattca tttttcaagc ggtagaaata gcctttttga gctaacagtt tttcgtaagt
    33781 acctatttca acgatttttc cttcatcgat cacaacgatt ttatctgcct ctttgatcgt
    33841 tgaaagccga tgagccacaa taaaagtagt gggtgtttct gtcaggtaat tcagggcttg
    33901 ctggattttt ttctctgact ggttatctaa agctgaggtt gcttcatcta gcaaaatgat
    33961 ttttggctga cgtatcaacg ctcgtgcaat ggagatacat tgcttttggc caccagacag
    34021 cttgttgcca tgttcgccta ctaaggtatc cagaccatca ggaagctggt caatcagtga
    34081 agaaagttgc gcagcttcga tgacttcatc taaacgttct tttgatacgt ttttcaagcc
    34141 ataagtgatg ttctctttga tcgaagcgga gaacaacaaa gtggtttgag gaacaacggc
    34201 taaataattt cgcacacttc gcatatccag ttcatccaaa ggctgatcat ctagaaaaat
    34261 ttttcctgaa gtcggctgga taaatccaat caataagtta aggagtgtag attttccaga
    34321 gccagatggc ccaacaaaag caattgtttc tccagctttc acctctagag aaaaatgatc
    34381 gataatcggt ttgagtgagt ccggataaag aaaagaaaca tcctcaaaac gaaaatttcc
    34441 atccaaatgt tggatttttt tcttaccgtg ataagtttct acatcgccag caatcaacag
    34501 ttcgccaata gaagtgatag attccatgcc ttttgaaatc gtaggaatca gcgtgatcat
    34561 tccagaaacg gaattgacga tcgtcgtgaa atacgtctga tacatcacga catcgccggg
    34621 ttggatgcgc ccttgtaaag ctaatatccc agtgaaaatc aaacaaatca gctgaaatat
    34681 ctgaaaacta gcccagctga ctgatccgaa aacagattgc aggatatcta agtggaatcc
    34741 tttttcatag atttgagtaa aatgcttatt taagcgagtt gtttcgtgtt tttctaaccc
    34801 atgagctcga gcaatcggtg ctaattcgat cacttcgatc acttttgcag aagtctcttc
    34861 catttcttgc ctgaagtctg agttggactg tctgatcctt ttacggaaaa agagagtaac
    34921 gaatgcggcg agaggaacag tcaataagaa aaacatgaaa acaacaggac ttttataaag
    34981 cgtgatacct aaagctacga ataaattgac agcgatattt aatccgctga caaatacttg
    35041 agaggacaac gtttggatag cttctacatc acggatgatc tttgactgga ttcgtccaga
    35101 ttgcaattct ttttgataag gaatagatag ttcttggatt ttcttgataa gggtcgctct
    35161 tagtccagct tctacttgac ggatcgaagt gctatggtat tttacatgaa aataattcgt
    35221 caatatattt tgaccaatca gacaagccat caaaaaagcg ttgaaataaa tggcctgcaa
    35281 ctgagaagaa tctttcgatg aagctgcatt gattacattg gcaatagcga tcggcattaa
    35341 ccaagatgga agatgtttga taataaaaaa gagtcctgac atggcaaaac gccaataatt
    35401 tccctgataa agatgcagca atgtctgaaa agcatttgca tgatattttt gataactttt
    35461 ctgtattcgt tcctccataa tcttctcctt aacaatcttg gtaaataaac tgcaagtgtt
    35521 ctattgcttc aggaacataa atctcaggag tagcttcaag agtggcgtac attagaggac
    35581 gctcatattt gatataatgc agtaccgggc ggaaatctaa acagcccttg ccaacaggta
    35641 ccatgcggat ctttccattt tccatgatat agtctttgag atgaagcgct gtaatataac
    35701 catgtaaatt gtccaaggca cctcggatca ctttttcctg atcagcatga ttttctttat
    35761 gcatcaaatt cgcacaatct aaaatgattt ttaaatttgg tgaagctact tcatcgatta
    35821 aacgtttagc taactgatag ttatagattg gatgattgat ccctgcttca acagcaacag
    35881 ttacgccaaa attttctgca tgagcgacta attcaatgat agaatttctg gcgatctgat
    35941 aagcttcttc agtaaaattt tcttttgtgt aaccgacaga gacactaccg gtttccgtag
    36001 caatcatctt ggcttgatag gcagagcaca gagcaatttg ctgtttgaaa gttgccaatg
    36061 cttgtgcacg aacttcttga tttttgctgg ataggttgat atagcatcct aaaatggaaa
    36121 gttcgatatt gtttttttgg aagtattcac caaaataggc agcactccca agtgtcatgt
    36181 cagttaattt aagttgttca ggaaatgctt tttgtggtgc aaattggata tgggacaagt
    36241 gaagctgtga tactttttga acgatttctt cacgatttcg aacatctaaa tcgtgggcac
    36301 ggatgcctaa atttaatgga aacatctcaa actccttttt gtaaataatt agcaagaggc
    36361 agacgggatt cttttatcaa ttcaacaatt attcgggcaa ctttatcagc gccttctgga
    36421 ctaaagtggg tattgtcttt tagtccttct gggtaatttt tacaagtatc aggcataaga
    36481 tgcaggtaga atttttttgt ctcttctttt gagaaggtct ggaacaattc ttgtgatctt
    36541 gagaacacat ctaaaagagg aatatggtgt ttctcagcaa atgcacgcat cgcttttgga
    36601 tagtctccaa gtatatctgg attcaatgtt ccgttttcta agtaatctcg tcgcgtcaca
    36661 gatgtcagta agaccatttg gacgttcgct ttttttgcgc attcaaaaaa tatctgcaga
    36721 ttttttaaat agctaccgaa gggaagcgta cctctttctt cttggatttt ctggtcgtta
    36781 tgtccaaatt gtacaaataa gaaatcattc ggctgaaact gtgaagctag acgggctaac
    36841 cttccttcct ggataaatga tttggtgctg cgtccattct ttgcttggtt attgatctgg
    36901 atctcgggtg aaaaataagc agagattttt tctccccaac ctgtctcggg acgcttgttt
    36961 tcatcttttt ctgccgcagt agaatcaccg gcaatatgga tagtaggcat gttatctctt
    37021 ctttcataaa ggtttatagg caatgaagca gaacagcaag agaagaagac ggagcaa
//
LOCUS       DACDXO010000022        36912 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E23605_2017
            SAMN12639989-rid9881463.denovo.022, whole genome shotgun sequence.
ACCESSION   DACDXO010000022 DACDXO010000000
VERSION     DACDXO010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639989
            Sequence Read Archive: SRR10023389
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 36912)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36912)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741914.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:42:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,972
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 33 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 17 of 73
            Pseudo Genes (multiple problems)  :: 10 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36912
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639989-rid9881463.denovo.022"
                     /isolate="E23605_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..1447
                     /locus_tag="IWM35_001281"
     CDS             <1..1447
                     /locus_tag="IWM35_001281"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HAP9919142.1"
                     /translation="DESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYAIAP
                     EYLFNVLADQAKQATFQLNDLNKAFVQLASTYNQFNGLFDDVDLQSKKLGTDEQQRNV
                     TITEVIKKLNDVDVLEHDGDVIGDAYEFLISQFASEAGKKAGEFYTPHMVSDMMAQIV
                     TLDQKERRFFSVFDPTMGSGSLMLNVRNYLTHPDNVKYHGQELNTTTYNLAKMNLILH
                     GVDAEEMNLRNGDTLNKDWPTDEPYTFDAVVMNPPYSANWSADTTFLDDSRFNRYGKL
                     APKSKADFAFLLHGFYHLKETGTMAIVLPHGVLFRGAAEGVIRQKLLEDGSIYAVIGM
                     PANLFFGTSIPTTVIVLKKNRQTRDVLFIDASREFVKGKNQNKLSEENIQKILETYAE
                     RKDVEKYAHLATFDEIKENDYNLNIPRYVDTFEEEEPIDMVHVGNDIKKIRQEQQVLE
                     KKLLEAISSLQTTPENEAWLQGALEVFKHEQ"
     gene            1437..2654
                     /locus_tag="IWM35_001282"
     CDS             1437..2654
                     /locus_tag="IWM35_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HAP9919143.1"
                     /translation="MSNDTQPEIRFPGFTDDWEQRKLGDFVVDYVEKTSVQNQFPMLT
                     SSQQKGIVLQEDYFANRQVTTENNIGYFVLPRGYFTFRSRSDNDVFVFNRNDIIDRGI
                     ISYFYPVFTLKSADSDFFLRRINNGIQRQLSIQAEGTGQHVLSLKKFKNIVAMFPSEE
                     EQQKIGTFFKQLDDTIALHQRKLDLLKETKKGFLQKMFPENGAKVPEIRFPGFTEDWE
                     ERKLGELGKTQSGIGFPDAEQGGSEGTPFFKVSDMNNIGNEYEMRNANHYVSNEQIER
                     KKWKPIKDVPAIIFAKVGAAIMLNRKRLVTSPFLIDNNTMAYLFNNTWDIYFGKILFE
                     TINLPRYSQVGALPSYNSSDIENISVKVPVKDEQQKIGTFFKQLDDTIALHQRKLDLL
                     KETKKSFLQKMFV"
     gene            2667..5816
                     /locus_tag="IWM35_001283"
     CDS             2667..5816
                     /locus_tag="IWM35_001283"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HsdR family type I site-specific
                     deoxyribonuclease"
                     /protein_id="HAP9919144.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWVNLRQ
                     KIISNNQAELNDFPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIEREDSQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIVQIELK
                     QVTAKDGVYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNEKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKTLMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGISSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVVDECHRALSAENMEEIKKMFPKSTWFGFTGTP
                     IFPENRKQAKGQLARTTHDQYGEVLHTYTIKNALEDGSVLGFQVEHENTVEPTSLENK
                     IYRKLKEVETYAEYSSEQINRMIDQMEPVKKESYLDPAVFEADEHIQKVIHKMFRPDN
                     AYTKFDFRNGRPTKSAILTTSSIDMAKKYYRAIKEMTKEPDWLTKEFPNQLIREGRTM
                     EDPDFPRIAITYSLEENSRDSSAQQEEMQKIIEEYNQYYDTSWSLADIERYNGDINNR
                     LARKRAEFKQFGKQIDLAIVVDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTY
                     PDKTKGLIVTFRKPATMEKNVEDATKLYSKAKEESGLIYPSYQESKKRFKQAYGKLKE
                     VSSMESIDEHTPLEIRIEYVKAFQELNRAYESLVTYDDYNDDMVKSPTLNNQIQLLEE
                     QIGVYETVKGSLIEEEPEKEGEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSD
                     AREEIEKALAKLNKTEGVKEVYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDDLIHV
                     FSNEWFVSEKELHASNLQYLPGEDPIPNMKAIINSKDYEGYKAKHPEAKPFKYPQEMK
                     RAWRKMLDDELIPLENELR"
     gene            complement(7751..8416)
                     /locus_tag="IWM35_001284"
     CDS             complement(7751..8416)
                     /locus_tag="IWM35_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9919145.1"
                     /translation="MNTGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDL
                     EMLSLLHHTLGLPIDFPSIESATESIEPTLPDEPLVNKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETIDRFNPFLKPQIGYTTLPVGYVDNSIERWVCNDIFGHGTW
                     LSFHAGEVEKEKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILSQDYDDALKRSPQEP
                     DRH"
     gene            8542..10578
                     /locus_tag="IWM35_001285"
     CDS             8542..10578
                     /locus_tag="IWM35_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HAP9919146.1"
                     /translation="MIIKKKFTVLLTIIVTGIMSGFALVSINKQVLYRTNEFFHQTDT
                     YTPIRLPQHFIGQEEYEQLIMVFENVSSETELSYMKKTVNESNEVDTSWNFKIQPKLE
                     MTLYYQGDVKSSHEKVPYSNTSFRVTSIEESIKIKDFEGEFFLMTREKEAYERFISLF
                     TDRFNQAFHTDYATNIFEDFENSYSYSVISNTDVYNYRVYLTIGLILLFLLLSSFFLL
                     MNKEISLLLNNDFSLHQVLWCLLGKKKIIITFIIAGIIGGFIFMYYKEQFLYLFQMYV
                     FLFSGIYGISLISLLLVEKIASLREAFNYIQYILFTFGLLFSLLLTVANVELATIVFS
                     SSQLAVSHEPPKEIAGEDYHVFYPVTIGKNHVDFIYSNRFASAADQELYETLNKNGSI
                     LVNVSDYLNEENEQFGRGIKINLNYLKKFPLIDVSGRLIQITEKETHPVILIPERYRH
                     TDLKNDLAQITEYYQEISEKEPKFLFIKNNQPIYTFIPSIPWIEHYPVVEILTLKNSM
                     YLERNILSGEIYPPLKIKIGSLSKERLFELIEESQLRDNLQLSFPYTQTEEIAVKVLS
                     RSFHYLIQIFLLTFFSFFLLSYVMLCIYFVYNCRKIAIFRSSGYSLFETYKDFFMMNL
                     IKWGTTSVIFLFLIEREPKYLFNIFFFSFIDFILSVVFILSTEKKSQLLLMNGG"
     gene            10582..11220
                     /locus_tag="IWM35_001286"
     CDS             10582..11220
                     /locus_tag="IWM35_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9919147.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDLVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFDQETKQLTGREKRLIKRNTISFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKDKVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            11248..11676
                     /locus_tag="IWM35_001287"
     CDS             11248..11676
                     /locus_tag="IWM35_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9919148.1"
                     /translation="MKKAVIICALGIGFVGGNAMSSEVEANSVHGEIYYKSLKPGEIH
                     FDAKGNIIKSNLPVDREISRTAINHSSGRWVYYSDTTCTRKTGHSNHYSRVYNRHGAK
                     AKVGNSIRSANATKGVWAYASATGNRTDTFSCWYNPTGWY"
     gene            12133..13044
                     /locus_tag="IWM35_001288"
     CDS             12133..13044
                     /locus_tag="IWM35_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP9919149.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTASSK"
     gene            13261..13698
                     /locus_tag="IWM35_001289"
     CDS             13261..13698
                     /locus_tag="IWM35_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9919150.1"
                     /translation="MEWFCKFLLFLEVIHVYGFVFLTNKKAGSTMDHLYIKYIHQFSV
                     LVLGTLIIAGIVAFIIQGRDILTVHQEFFSYAFGIPLIAIRSDNKKWKKRYYILLLFL
                     CFYLPPKIFTLLGIQCLSFSFISYIIVLIFLSFIVWMFYLVKK"
     gene            13793..14677
                     /locus_tag="IWM35_001290"
     CDS             13793..14677
                     /locus_tag="IWM35_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HAP9919151.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPESINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSTLLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            14901..18068
                     /locus_tag="IWM35_001291"
     CDS             14901..18068
                     /locus_tag="IWM35_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9919152.1"
                     /translation="MNQCVKKLIASILFVFPLLMVTSVFADVEKTNESTQSENQSFDK
                     TTFSEESITSETTESQSEASTNKRQEENKTSETSEVAEQTSDSSEAALARAVSADGYS
                     EAATTEELAQLLADESVTKIRLIQPLTLDRELEIKRDIVIDFGGFAHNFGTHHIYINE
                     IPQSIEFQNFKGTAAHPGGLTPNIDGNAIIIAYMTDFWGSRYHFTGEIKFTGTLDLYD
                     GSKLGLIYAPRATVTLDGVSGVLDVQPVKEGLNAAPGKAYFCRSYQLNVINGSQLYGP
                     YLGKFYGFLDSGDETGSGKTAPGIHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVMF
                     EVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSKVTVSNGGKITINTATTGGFRLQGD
                     SSQIHVSSGGQISVKMAGDDDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSAV
                     RFENGTQKLTVTNGGSLRVRNTGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFTV
                     TGEKSNIDIRADSGATVDTTGDIDLNFTAGEKTYLVMVGKTGEDKTGIISGDVLNVDL
                     ESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKTGATFDNFEKDPD
                     YFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRVP
                     TNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSSVEITALTQGQTDEN
                     EGAGYNPWGEGEQGGLFEVQVPNDERLKTEDQVQVVAAKKVRGGQEVTELDSAKTTVD
                     VIPPEPANLSTTLINAASRTITGTGEAGALVSLKKNQAVLSETTIDDTGEFVLSIPEN
                     QLFAGDVLEIVLNDQAGEATAKGVQNKPSTNDEIGNHNPSDTPVVYHDAPPFAPATKI
                     VVEGGLSFLAPSKLDFGQIKATGLTQEAFGQMDIQEKLWIYDQRETKNPWILTIKLSQ
                     PFTNDAGFSMQDVLYFKKGEEYQLVNEEELEIVRGQNSTNVQADYSEYLNNGRAFKLV
                     LTKENQIAGNYSAEITWTLADAPTE"
     gene            18088..18633
                     /locus_tag="IWM35_001292"
     CDS             18088..18633
                     /locus_tag="IWM35_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 domain-containing protein"
                     /protein_id="HAP9919153.1"
                     /translation="MNRIWNKSHRNNCSLLMILLLGFILWQTPVHASEFNFAVDFELP
                     ANQIDKNKTYINVRMEPGKTQEIPYTVFNRTDKSVTVETLIHSATTNSNGVIEYGESK
                     SKFDETLSYDMKNLAKTVGEITVPPQSAVTETLSLKAPEKELAGVVAGGITFQEKKEN
                     VSNKSTVGVVNQFSQVKAILI"
     gene            18661..19116
                     /locus_tag="IWM35_001293"
     CDS             18661..19116
                     /locus_tag="IWM35_001293"
                     /inference="COORDINATES: protein motif:HMM:NF023225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3324 domain-containing protein"
                     /protein_id="HAP9919154.1"
                     /translation="MKLKKAYADQSDGRNVIKANLQNPNASYMFGLTTSVEIYKKETK
                     EPYLSIKKEKIDVAPNTNFDLLVPLEGKKLAPGHYQAVVKGAWKEKRWNLHTDFEITD
                     DAADQLNEKDIDLKNQPETFDWRWLILVGVILIGLFAGLFILKERRKKK"
     gene            19113..19871
                     /locus_tag="IWM35_001294"
     CDS             19113..19871
                     /locus_tag="IWM35_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAP9919155.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPDEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QSGTDSTGAQTQVQNFVQVTDKSGNYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGMPSTVSGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEEEA"
     gene            complement(19957..20697)
                     /locus_tag="IWM35_001295"
     CDS             complement(19957..20697)
                     /locus_tag="IWM35_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9919156.1"
                     /translation="MKKYFIIEDNQFHSEWIIRELVNHHIENIVQLTSISQINQLIQL
                     GTIDNHDVFFIDIDLKQSLTGIDVAEQIRKKNAHSNIVFFTAYTNFAVEIINRKILPI
                     GYIRKDRNLSEQIRQVLEQINWKEQQIVNVDKIFIDQKGGSLVLYPREILYIESIKGL
                     KNQVMVKTTTEDKLVNTSLKQLKTELEKYAYFLLLKSYCINLSQLQKIDSAHYVLEFF
                     NGDSLLVGRKIFEKTKERFHDFQKTASS"
     gene            20758..22011
                     /locus_tag="IWM35_001296"
     CDS             20758..22011
                     /locus_tag="IWM35_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAP9919157.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGCIALMVVTLL
                     LPIPKVSFPYLVGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSLFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EEFKHDYKSLLFSLKITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRG
                     VFLKFLEKAESEKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITI
                     NLLEEKEGSRFSIANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELK
                     IEFSKQNHYFFVEMYLASRKSKSAY"
     gene            22333..22560
                     /locus_tag="IWM35_001297"
     CDS             22333..22560
                     /locus_tag="IWM35_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HAP9919158.1"
                     /translation="MLTQQLRELEQDESIHRKAYNQVPPKVEYSLTEEGKSLREILVA
                     LSVWEEERIEKQKASREEDTLLHTHGGYLNY"
     gene            22683..23009
                     /locus_tag="IWM35_001298"
     CDS             22683..23009
                     /locus_tag="IWM35_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HAP9919159.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLHLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEL"
     gene            23077..23790
                     /locus_tag="IWM35_001299"
     CDS             23077..23790
                     /locus_tag="IWM35_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HAP9919160.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYFVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PVFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEGKEIVTLITCGDMDAVTRLVVQGELESVTS
                     IKDATDDMRSAFNLETKTF"
     gene            24100..24951
                     /locus_tag="IWM35_001300"
     CDS             24100..24951
                     /locus_tag="IWM35_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP9919161.1"
                     /translation="MKIDKILNNNVVISKNGFGEEVVCMGKGLAFQKKIGDEISPEAV
                     QKEFVLRDSFAKNQFQQLMADIPAEEIEWVKQVVELAEDKLKVEFSPNIYLTLTDHLH
                     YAITRAKENIQLPNPLLFETKKFYPKEYQAAKEALLFVKQKTGVELSEDEAGFIAFHF
                     VNSQQGNEDMQVTMTATTMVRDILSIISKFFGIVFDEESLNYQRIITHLQFFAQRYLK
                     GEGSDEQDEFLYALVQGKYPKAFHCVERINEYLRQTQKQEMGIAEQIYLTIHIQRVVS
                     EKKSIQS"
     gene            25072..26937
                     /locus_tag="IWM35_001301"
     CDS             25072..26937
                     /locus_tag="IWM35_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9919162.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHWGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGKPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            26985..28394
                     /locus_tag="IWM35_001302"
     CDS             26985..28394
                     /locus_tag="IWM35_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HAP9919163.1"
                     /translation="MEQLLPNQLEGAYDRDGKGLSVADAMPGGKQRFAIIGSEEFDWT
                     IDQEKYIYPNHRGIDHYDRFKEDLALFAEMGFKCYRFSIAWTRIFPNGDEGTPNEAGL
                     EFYDQLIDECLKYDIEPVITISHYEMPLHLAKEYGGWKNRKLIDFYERFAQTVLERYS
                     SKVKYWMTFNEINSAFHFPALSQGLVKSNGAGEYQNIFQAWHNQFVASSKAVKIGHEL
                     RSDIQIGCMIIYATTYSIDANPVNQAATMIQNQEFNFFCTDVQVRGEYPAYTARTHKK
                     YGVDPEKLEQTEKDFQLLKEYPVDYIGFSYYMSTAINETDPQAATSEGNLLGGVKNPF
                     LEASEWGWQIDPEGLRIALNELYNRYQKPLFIVENGLGAIDHMEEDGTIIDDYRIDYL
                     RRHIEAMANAVADGVDLMGYTPWGCIDLVSASTGEMSKRYGFIYVDLDDEGNGTLNRS
                     KKKSFDWYKEVIASNGVNL"
     gene            28502..29203
                     /locus_tag="IWM35_001303"
     CDS             28502..29203
                     /locus_tag="IWM35_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP9919164.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQGILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            29362..29841
                     /locus_tag="IWM35_001304"
                     /pseudo
     CDS             29362..29841
                     /locus_tag="IWM35_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015510350.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
     gene            30689..31264
                     /locus_tag="IWM35_001305"
     CDS             30689..31264
                     /locus_tag="IWM35_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HAP9919165.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
     gene            31264..31704
                     /locus_tag="IWM35_001306"
     CDS             31264..31704
                     /locus_tag="IWM35_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HAP9919166.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVVAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFETLPVETYVGEYL"
     gene            31720..32421
                     /locus_tag="IWM35_001307"
     CDS             31720..32421
                     /locus_tag="IWM35_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAP9919167.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDKFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
     gene            complement(32909..33334)
                     /locus_tag="IWM35_001308"
     CDS             complement(32909..33334)
                     /locus_tag="IWM35_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9919168.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DTTNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGTDSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKETTVIFTKIGNYRLKDEDGNIYSL"
     gene            33708..34679
                     /locus_tag="IWM35_001309"
     CDS             33708..34679
                     /locus_tag="IWM35_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP9919169.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMKKIAEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISSRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHVIDGLIVFLSEEEWSGFEMLTKMSIPVIALNCPEGPAGIDTILVNDR
                     ESVAKVIAHHGEQGHKKIGFIAATQTDYVARERLAGAKEAVQNDPSIQLEVFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            34711..36108
                     /locus_tag="IWM35_001310"
     CDS             34711..36108
                     /locus_tag="IWM35_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP9919170.1"
                     /translation="MKKYQFPANFWWGSAASGPQTEGRISGDGKGENIFDYWYKKEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HRYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFELFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKSYHEMEQGGVI
                     GIILNLTPSYPRDESNPEDVKAAQIADAFFNRSFLDPAVKGEFPSELIEIVKELDLMP
                     VIHAEDLTVIKENTVDLLGINYYQPRRVKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEQGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDKNGFEA"
     gene            36267..36890
                     /locus_tag="IWM35_001311"
     CDS             36267..36890
                     /locus_tag="IWM35_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9919171.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWAEYKITANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLTVLLLTHDGDKVARLKNGKVITAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     VSSQKNESTGTSPSDSGSGQTITGSEENQASIITNESSQGQYQSYGQ"
BASE COUNT        12605 a         6008 c         7596 g        10703 t
ORIGIN      
        1 agatgaatcc ttagaggaat atgacacagt aagcaaacaa acaatgttgt atcgtgaatt
       61 actttcagat gaagaatcaa aagaagatct gattgccaca atcgttgata ttttgggata
      121 tgcaattgca ccagagtatc ttttcaacgt cttagcggat caagcaaaac aagcaacgtt
      181 ccaattgaac gatctaaaca aagcctttgt gcaattggca tctacctaca atcaatttaa
      241 tgggttattt gatgatgtgg atctccaatc aaaaaaacta ggaacagatg agcaacaacg
      301 aaatgtgacg attaccgaag tcatcaagaa attgaatgat gtagatgttt tagaacacga
      361 tggggacgtg atcggggatg cgtatgagtt tctgatcagt caatttgctt cagaagcagg
      421 gaagaaagcc ggagaatttt acacacctca tatggtttct gatatgatgg cgcaaatcgt
      481 aacgctcgat caaaaggaac gacgattctt tagtgttttt gatccaacga tgggttcagg
      541 ttcattgatg ttaaacgtac gtaactactt aacacaccca gataacgtta aatatcatgg
      601 gcaagaacta aatacaacga catacaatct agcaaaaatg aacttgattt tacatggtgt
      661 cgacgccgaa gaaatgaatc tccgcaatgg ggatacgttg aataaggact ggccaacaga
      721 tgaaccttac acatttgatg cagtagtaat gaacccacct tattcggcta attggtcagc
      781 agatacgact ttcttagatg attctcggtt taatcgttat gggaaattag cgccaaaatc
      841 caaagcagat tttgcgtttc ttcttcatgg gttctatcat ttgaaggaaa caggtaccat
      901 ggccatcgtc ttaccacacg gagttttatt ccgtggcgct gcagaagggg tcatccgtca
      961 aaaattacta gaagatggca gtatttatgc tgtgatcggt atgcctgcga acttgttctt
     1021 tgggacatcg attccaacga cggtgattgt gttgaagaag aatcgtcaaa ctcgagatgt
     1081 attattcatt gatgctagtc gtgaatttgt taaagggaaa aatcaaaata aactatcgga
     1141 agaaaacatt caaaaaatcc tcgaaaccta cgctgaaaga aaagatgtcg agaaatacgc
     1201 tcatttggca acctttgatg aaatcaaaga aaatgactat aacttaaata tcccacgcta
     1261 tgtagatact ttcgaagaag aagaaccaat tgatatggtt catgtcggaa atgacatcaa
     1321 gaagatacga caagaacagc aagtgctaga aaaaaaactt ttagaagcta tctcttccct
     1381 gcaaacaaca cctgaaaatg aagcgtggtt acaaggtgcg ctggaggtgt tcaaacatga
     1441 gcaatgatac acaaccagaa atccgtttcc caggatttac agatgattgg gaacagagga
     1501 agttggggga cttcgttgta gattatgttg aaaagacaag tgtacaaaat caatttccta
     1561 tgctaacttc atctcaacaa aaagggatag ttttgcaaga agactatttt gcaaatcggc
     1621 aagtaactac agaaaataat attggttact ttgtactacc tagaggatat ttcacgttcc
     1681 gtagtcgaag tgataatgat gtgtttgtgt ttaaccgtaa tgacattatt gatagaggga
     1741 ttataagcta tttttatcct gtatttactt taaaatctgc tgattccgac ttcttcttaa
     1801 gacgtattaa caatggaatc caacgacaat tatctattca agcagaggga acaggtcaac
     1861 atgttctgtc tctaaaaaag tttaaaaata tagtagcaat gtttcctagt gaggaagaac
     1921 aacaaaaaat cggcaccttc ttcaaacaac ttgacgacac gatcgctctt catcagcgta
     1981 agttagattt gctgaaggaa acgaaaaaag gattcttaca aaaaatgttc cctgaaaacg
     2041 gagcaaaagt gccagaaatc cggtttcctg gttttacgga agattgggaa gagcgtaagt
     2101 tgggagaatt aggtaaaact caatcgggaa ttggatttcc tgatgccgag caaggtggtt
     2161 ctgaaggtac tccatttttt aaagtttccg acatgaataa cattggtaat gagtatgaaa
     2221 tgagaaatgc aaatcattat gtatctaatg agcaaattga gcgtaaaaaa tggaagccaa
     2281 taaaagatgt gccagcgatt atatttgcta aagttggcgc agcaataatg cttaatagaa
     2341 aacgtttggt cacaagccct ttcttaatag ataacaatac catggcttat ttatttaata
     2401 atacttggga tatctatttt ggtaaaatat tgtttgaaac tataaatttg ccaagatact
     2461 cccaagtagg ggcattgcct tcttataaca gttctgatat tgagaatatc agtgttaagg
     2521 ttcctgtaaa ggatgaacaa caaaaaatcg gtactttctt caaacagcta gatgatacga
     2581 tcgctcttca tcaacgtaag ttagatttac taaaagaaac gaaaaaaagc ttcttacaaa
     2641 agatgtttgt ctaggaggga agaagaatga aaattagtca ccgtgaggaa gcagaagtcg
     2701 aagaacagtt gattcgtgta cttggagaag ggcataatca gtggacgtat cgtccggatc
     2761 taaaatcaga agaagacctt tgggtcaatt tacgccaaaa aattatctca aataatcaag
     2821 cagaactgaa tgattttcct ttgaccgata aagaatttga aacgatcaaa acggaattac
     2881 tgctgcgaac gaaaacccca tttgatgctg cgaaatggtt aaaaggtgaa aatgggatgg
     2941 ctcggatcac gattgaacgg gaagattcgc aattgggttc tgtctcactt atcctttatt
     3001 ccaatcaaga cattggtgga ggaatctcta cttatgaagt ggtccatcaa attgcaaaac
     3061 gtggaagtaa catagaagca cgggatcgac gttttgacgt caccttatta atcaatggat
     3121 taccgattgt ccaaatcgag ctgaaacaag taactgcaaa agatggtgtt tatcaggcat
     3181 ttaaccaaat caaaaaatat gcagaagaag gtatgtttcg gaacaatatc ttttcgacct
     3241 tacagttgtt tgtggtttcc aatgaacaaa ccactcggta ttttgcaaat gccttaccga
     3301 aagacttgca ccctaagttt ttatttagtt ggcgaacgaa agacaatgaa aaagtagaaa
     3361 acttatatga gttttgtaaa caagtgttaa atatcccgga tgctcatcgt ttgattgcgg
     3421 attatacaat cgtcagtgaa gatcaagaca ataaaaccct catggtgtta catccttatc
     3481 aagtacatgc gattcaagcc ttgtttattg ccgcaaataa acatcaatca gggtatgtgt
     3541 ggcatgcgac tggttcagga aagacgttga ctagttttgt atctactaag cttttagcac
     3601 gaaaatcggg gatcgatcga acgatcatgt tagtggaccg caaagattta gataatcaaa
     3661 ccaccacaga gtttacaaaa tttgcttcgg aatttaatac tgggatttct tccgggaacg
     3721 ccaaagcgaa tagcttgatc gttggaacgg gaagtgccaa agaattaagt gaaaccctgt
     3781 tggcagatgc caatgcaaat gttgtaatca ttacaacccg tcaaaaacta gatgcggcgt
     3841 tgaaatatgc aaagaaacaa gaagagaaaa aaggaaccaa tcgcttccaa aaattaatgg
     3901 gccaacatat cgtgtttgta gtagatgagt gtcatcgagc gttaagtgct gaaaatatgg
     3961 aagaaatcaa aaaaatgttt ccgaaatcca cttggtttgg ctttacaggt acaccgattt
     4021 ttcctgagaa tcggaaacaa gccaaagggc aattggcacg tacaacccat gatcagtatg
     4081 gagaggtctt gcatacctat accattaaga atgcgctaga agatggctct gtcttaggat
     4141 ttcaagtaga gcatgaaaac accgtggagc cgacttcact agagaataaa atctatcgga
     4201 aattaaaaga agtagaaaca tatgcagagt attcatcaga acagatcaat cgtatgattg
     4261 atcaaatgga acctgtcaaa aaagaaagtt atttggaccc agctgttttt gaagcagatg
     4321 aacatatcca aaaagtcatt cataaaatgt tccgtccaga taatgcctat acaaaatttg
     4381 attttcgaaa tggcaggcca acgaaatctg caatcttaac cacgagttcc attgacatgg
     4441 ccaaaaagta ttatcgggcc atcaaagaga tgacgaaaga gcctgattgg ttaacaaaag
     4501 agtttcccaa tcagctcatt cgtgaaggac gaacgatgga ggatcctgat tttccacgaa
     4561 tcgctattac ctattcatta gaagaaaata gccgagattc aagtgcccaa caagaagaaa
     4621 tgcaaaaaat tattgaggaa tataatcagt attatgatac ttcctggtca ttagcagaca
     4681 ttgagcgtta taacggagac atcaataatc gtttggcacg aaaaagagcg gaattcaaac
     4741 aatttgggaa acaaatcgat ttagcgattg tagtggatcg tctattaaca ggttttgacg
     4801 cccctacgat tcaaacccta tttgtggatc gtaatctaga gtatgcagga ttgatccaag
     4861 ctttttcacg tacgaatcgt acgtatcctg ataagacaaa aggattgatt gtgacgtttc
     4921 gtaagccagc gacaatggag aagaatgtcg aggatgcgac aaaactgtat tcaaaagcaa
     4981 aagaagaatc aggtttgatt tatccaagtt atcaggaatc caaaaaaaga ttcaaacaag
     5041 catatggtaa gttaaaagag gtttcatcca tggaatccat tgatgaacat acgccacttg
     5101 aaatccgtat cgagtacgtc aaagcatttc aagaattgaa tcgtgcttat gaatcgttag
     5161 tgacttatga cgattacaat gatgacatgg tgaaatctcc aactttgaac aatcaaattc
     5221 aacttttgga agaacaaatt ggcgtgtacg agaccgtgaa gggttcatta atagaggaag
     5281 aaccagaaaa agaaggagaa gatttttcaa cgatcgaatt ttatagtgaa aataccacta
     5341 agttgtatga tattgattca acttatattg atcaattact aggaacttat gctgcgaata
     5401 gttcagatgc acgagaagaa attgaaaaag ctttggctaa attgaataaa acagaaggag
     5461 tcaaagaagt ttaccgacaa attttaaatg catttgatgt ggggacttta gatagtaatg
     5521 aagatatctt tgtcattaaa cgtcgctatt ttacacaagc gagtgatgat ttgattcatg
     5581 tcttttccaa tgagtggttt gtttctgaga aagaattaca tgcttctaat ctgcaatatc
     5641 taccaggtga agatccaatt cctaatatga aagcaattat caacagtaag gattacgaag
     5701 gatataaagc caaacatcca gaagcgaagc catttaaata cccacaagaa atgaagcgag
     5761 catggcgtaa aatgttagat gatgagttga ttccacttga gaatgaattg aggtaaacga
     5821 gaagagttaa gaaaattgta cgattcaaag atggtagtca agtggaagtg tagaaaatag
     5881 ggatagatgt cttttatttg ttcacatttt ataacgaagt atttaagaca gctattgaat
     5941 aatgtttcct ccaccttttt cattcattta ccccaccccg ttgttatatg accgtgtttt
     6001 gagaggaacc aataaaaagc ataggctaac taggggttcc tttttctttt gtttttttgg
     6061 aatccgctta agtgaaatgt atctgaaatg aaacggagat aaagtgattt tttgctataa
     6121 tgacaaggtt ttagctgttt cagataccat gtaacccaaa atagtttatt gagaaaatag
     6181 gagagtattt gatactaact agtaggaaaa aatgagataa aaattaaatg gaatcatttc
     6241 aggtaatctc aaataaaaaa atatacagtc ctgtggcact ttacctatgg gactgtatat
     6301 tttttactct cagcgggctt gttttgactc cgtgtctgac tccgtcgtat tgattttaaa
     6361 tgatattcga tgaccattta acaggtcatt tttgaaattt ttgtctctat atgactcagt
     6421 ttgataatta ttgatagatc aatttgggtc tatatcgaac aagggaataa tccaataaaa
     6481 aatgatttag aatatcaaaa taacaaaaaa gaggactacc tgtattcgtc ctcttttttg
     6541 accagatttt gaccagtcta atttacattc taatatattc atatgagcta attttttgat
     6601 taaatggtaa gtttttcctt gtttgtttta gtttaaacta gtgtgtaatt taaaaatcta
     6661 tatggaaggg tctgtggggt aatacttaaa taaaggctag gaacctgata acatcaaggt
     6721 tcctagcctt tttataataa aatgaagcga aaataatttc ttttaaagag ccgagattat
     6781 cagctatata agagtgaatt tgatgcaaaa aacagagccg aaacagtctt ggtgttcgta
     6841 gctctgctta tatgtttcga atttaagaca aagagttggc aaaagatgtt gataaggaag
     6901 ataagataac tgtgttatat aaactaggta actaactgta acaagctaat tttaagagat
     6961 tcttattaga agacgtttag gaatataact aagaaaatga ttatacaggt aattgtcatt
     7021 tcaataatgt tttctaattt tcgcgtcttt gttttaagaa aaaggccaga atcagttgga
     7081 aaaaagttcg tacgatagtg aagaggggca aaatttccta ctaatattcc cactaacaag
     7141 agataaaacg gccaggttgg tgtgattcct ctataaaaaa atgcccatgc taccgaaatc
     7201 ccatagatta acaactgaac aatttttaca atgaaaaaat tgttctgcca ttactcaaag
     7261 tctcttcctc cttgtatcgt ttatttgttt cattttatca ttatgatagg cgcttgaaca
     7321 atccattttg tattttattg agtactcttg gataaactac cagcgattat catatttatt
     7381 tatgttactg taagccatca tataaaagta aatgagcaga tcatagaaat ataaaaaaga
     7441 actaaaattg atctaaatta tgtgcagctt gactacgtac caatctatat tacttactag
     7501 attggtcgtt aatctattcg gatcgaatga gcaaaatatt tttgtgaatg tccatccttg
     7561 attatgattc caaaaagaat cattgggagg ctgtggcgta aatattatac tataagccag
     7621 aaatacctat cattctgaac ttttttattt aggaactttc atctcaaggc tttttcaatt
     7681 aatttgtacc cctgtttttt ggataagatc atgtgcattg attttttcta tctctacgtt
     7741 catctcctac ttaatgccta tctggctcct gagggcttct ttttaaggcg tcgtcatagt
     7801 cttggctgag gatttcttgg taatctgagg gaatggcttc ccaagtgagc aggcgaacgt
     7861 tcatgacgtg ttttcctttg tgtttgatta cagcatggtt tttctctttt tctacctctc
     7921 cagcgtgaaa agacagccat gtaccatggc caaagatatc attacatacc caacgttcga
     7981 tggaattgtc cacgtatcct acagggagtg tggtatagcc gatttgtggt ttcaaaaaag
     8041 gattgaatcg atcgatcgtt tcatttttgt attgataatt gataagaaaa agatagacca
     8101 aacaaaagac gagcagaaag aaagatagtg tctttttgag tgagaagggt tgttttgatg
     8161 aataagattt cttgttaaca agaggttcat ctggcaatgt tggttctatt gattcggtag
     8221 ctgattcaat agaaggaaaa tcaatcggca gacctaaagt atgatgtaaa agtgacagca
     8281 tttcaaggtc tggcattgta cgagaatttt cccaactaga aatcgtttta tcggaaacac
     8341 caattttttc gcctaattct ttttgcgtaa gcttacaatt tctgcggtaa gttcgtattt
     8401 tttctccggt gttcatcttc ttctcctttc gattctacag tatatagatt tattctacat
     8461 gacctataca cttgatagat acgttttttt gatgaaaaac ggtatgattt tatcaaagaa
     8521 tcatttaagt aagggagagt ggtgataatc aaaaaaaagt tcacagtttt actcacaatc
     8581 atagtgaccg gtatcatgag tggttttgcc ttggttagta taaataaaca ggtactctat
     8641 cggacaaatg aatttttcca tcaaacagat acttatactc cgattcgatt gccacaacat
     8701 tttattgggc aagaagaata tgagcagcta atcatggtat ttgaaaatgt ctcatcagaa
     8761 actgagcttt cttatatgaa aaaaacagta aatgaatcca atgaagtaga tacatcttgg
     8821 aacttcaaga tacagccaaa gcttgaaatg acattgtatt atcaagggga tgtcaaatct
     8881 tctcacgaga aggtacccta ttctaatacc tcctttcgcg taacgtccat tgaagaatcg
     8941 atcaaaataa aagattttga aggtgaattt ttcttgatga cgagagaaaa agaagcctat
     9001 gaacggttca tttcactatt cactgatcga ttcaatcaag catttcacac tgattatgcc
     9061 actaatattt ttgaagactt tgaaaatagc tattcgtatt cggttatttc aaatacagac
     9121 gtatataatt atcgagtgta tttaactatt ggattgattc ttttattcct tttgttatca
     9181 tcctttttct tattgatgaa taaagaaatc agtttattgt tgaataatga cttttcatta
     9241 catcaagtat tgtggtgttt attggggaaa aagaaaatca ttattacatt cataattgca
     9301 ggaatcattg gtggttttat ttttatgtat tataaggagc aatttcttta tttgttccag
     9361 atgtacgttt tcttgttcag cggaatatat ggtatctctt tgatttctct tttattagtt
     9421 gaaaaaatcg cttcgttaag agaagcgttc aactatatcc aatacattct ttttacattt
     9481 ggattgttgt tttctcttct tttgaccgtt gccaatgtcg aattagcaac tatcgttttt
     9541 tcttcttcac agttagctgt ttcccatgaa ccaccaaaag aaatcgctgg tgaggactat
     9601 catgtatttt atccagtaac catcgggaaa aaccacgtgg attttattta ttccaatcgt
     9661 tttgcatcag cagcagatca ggaattatat gaaactttga ataaaaacgg aagtatatta
     9721 gtcaatgtat cagattactt gaatgaagaa aatgaacagt ttggcagagg gataaaaata
     9781 aatctgaatt atcttaagaa atttcctttg attgatgtct ctggaaggtt gatccaaata
     9841 accgaaaaag aaacgcaccc tgttatattg atccctgaac gatatagaca tacagattta
     9901 aaaaatgacc tggcgcaaat aacagaatat tatcaagaaa tcagtgaaaa agaaccgaaa
     9961 tttctattta taaaaaacaa tcaacccatt tatacattta ttccttctat cccctggatc
    10021 gaacattatc cggttgttga aatactgaca ttgaaaaaca gtatgtattt ggaaagaaat
    10081 attctcagcg gagaaatcta tccaccgtta aagatcaaga taggctcttt gtcgaaagaa
    10141 aggttatttg agctgattga agaaagtcag ttacgtgata acttgcagtt atcatttccg
    10201 tatactcaga ctgaagagat agctgtcaaa gtgttaagca gatcatttca ttacttgata
    10261 cagatatttt tactcacttt cttttctttc ttcttgttga gctatgtcat gttatgcata
    10321 tattttgtct acaattgtcg gaaaatcgct atctttcgtt caagtggtta ttcgctcttt
    10381 gaaacgtata aagatttctt catgatgaac ctcatcaaat ggggaacgac aagtgtaatc
    10441 ttcctttttc ttatagaaag agagcctaaa tatcttttta atattttttt ctttagcttc
    10501 attgatttta ttctttcagt tgtttttatt ttgtctacag aaaagaaaag tcagttattg
    10561 ctgatgaatg gaggttaaca attggagccg ttgatagtga taaaaggtgc gaaaaaaatc
    10621 attcgaaaca aaacgattct tcatttggat cgtttcactg cggatgctgg ggatcttgtg
    10681 actgttgttg gagaaagtgg agcaggtaaa agtacgttat tgaatatatt aggattgaat
    10741 gatcagttca gttcaggaca attctatttg ttcgatcaag agacgaaaca attaactggg
    10801 agagagaagc gcttgataaa aagaaatacc atatcttttc tttttcaaga ttttgggttg
    10861 attgaatatg aaacaatcga ttttaattta gatattggat tgaagttttc gaatttaaat
    10921 gtttcagata agcgaacagc aaagtatgag gcattgaaaa aagtaaatat aaacaagaag
    10981 ctaagtacgc ctatctacac attatctggt ggtgagaagc aaagaatcgc ctgtgcgaga
    11041 gtcttattga agaatagcaa aatcatttta gccgatgaac cgactgggtc attggataaa
    11101 agaaataagg ataaagtagt tgaaatctta tcgcaacaag cttcccaagg tacgttagtt
    11161 atcgtagtca cgcatgatcc atatctgacc aaaataggaa acaagaagat catgctttaa
    11221 aaatcaaaaa ggaaaggatg tcgttttatg aaaaaagcag ttattatttg tgcactaggg
    11281 ataggttttg ttggaggaaa cgctatgtca tctgaagtgg aagcaaattc agttcacggt
    11341 gaaatctatt acaaatcttt aaagccagga gagattcatt ttgatgctaa aggtaatatt
    11401 attaagtcaa atttaccagt agaccgggaa atttctagaa cagcaattaa tcatagctct
    11461 ggaagatggg tttattatag tgatacaact tgtacgagaa aaacaggtca ttctaaccac
    11521 tatagtcgtg tctataatcg tcacggtgca aaagcgaaag ttggaaactc tattcgatca
    11581 gcaaatgcaa caaaaggagt atgggcctat gcaagtgcga caggaaatag aactgacaca
    11641 ttctcatgtt ggtacaatcc aaccggttgg tattaagtat ccaaaagttt atataaaata
    11701 gaaaaaacat ataatgaaac agcgttaaaa gttatttttg gtagttttaa tatattcatt
    11761 tttagttgta taaaaatata tcataataaa aacaacaaaa aaatgtctgg aaaatcgaac
    11821 ataaaagtgt ctgatttgaa tatcccagca gcggaagaag aaaaacaaaa tcgataacat
    11881 ttgttctaag aattagttac gttaaatacg gaacgcaaga tagacttgaa aaacggtaac
    11941 aaaagaagta gtatattaag gtgtgatgtg ctacttgaca tttattctgc tgagcgaact
    12001 ttctggattg tgtagcctta tatctattca ggacgtatcg agcagattag taaaggtgtt
    12061 cttgatgatt ttcatcttga acacctttgt ttttagcaat tcgcaaaaaa cgaaaagtag
    12121 gagaattgat aaatgaaaaa aagattttta gcacttgcca tcgtactagg cactggctta
    12181 ttgtcaggct gtacgaatgc cggtgaaaaa acagccgtta gttataaagg cggtacaatc
    12241 agcgaacaag aagtaatgga tagcttgaaa aaaatgcaag gagcagattc cgctgtgcaa
    12301 cagttgatcg tttatcaagt atttgaagat aaatatggag atgacgtatc aaccaaagaa
    12361 atcgattctc aatatgatca aacaaagaaa caattgggtg attcatttga ttcacaattg
    12421 aaatctgcag gttacacaga acaaacattc aaagacagta tcaaacaaag tctagccttt
    12481 caagaaggtc taaagaaaca tatcaaattg accgatgaag atttaaaaac ggcttgggaa
    12541 tctttccatc cagaagtgga agctcaaatt atccaagtag caagtgaaga tgatgcaaaa
    12601 gatgtgaaga aagctgctga taagggagac gatttctcta aattagccaa agacaagtca
    12661 actgatacta cgacaaaaga agatggcgga aaagtgaaat ttgattccac tactacaacg
    12721 gttccggcag aagtcaaaga agcagccttt aagttgaaag atggtcaagt ttctgatgtg
    12781 atcacttcta ccaatgcttc aacttatact acggaatatt atgtggttaa aatggtgaaa
    12841 aaccaaaaca aaggcaacga catggataaa tacaaaaaag aattgaaaga aattgcaaca
    12901 gacacaaaat taagtgacag taccttccag aataaagtaa ttggcgaagt attgaaagac
    12961 gctaatgtaa aaatcaaaga caaagacttt gaaaatgttt tgagtacatt cacttcagat
    13021 tctagtacag caagtagtaa ataagcggta taaataaaaa atatgaacta tttaaatcaa
    13081 taaaactgtt catctaaatc tttttattag aactaaatca acagtagttt aattactact
    13141 tgatttagtt ctttttattt taaaaatttt ttttacttag aatccagcga ccctagatat
    13201 ttttctgtat aatcttaaat aaagaaaaca atatcaaaaa atgcacgtag aagtagataa
    13261 atggagtggt tttgcaaatt tcttttattt ctagaagtta ttcacgtata cggatttgtt
    13321 tttctcacaa ataaaaaggc aggaagtaca atggatcatt tgtatataaa atatattcat
    13381 caattttccg ttttggtgtt aggcacacta attatagcgg ggattgtcgc atttattatt
    13441 caaggccgtg acatattaac agtacatcaa gagtttttca gttatgcatt tggtattcct
    13501 ctgatcgcta taaggtctga taacaaaaag tggaagaaac gctattacat tctcttgcta
    13561 tttttgtgtt tttatctgcc acctaagatt tttactttat taggtataca atgcctatca
    13621 ttttctttta tttcctatat cattgtttta attttcctca gtttcatagt atggatgttt
    13681 tatcttgtca aaaaataaat gaatgtttca tgaacagtac ttcatcacag cctaattttt
    13741 ttctttatgc tatcgttata ttaaagaaat caatcagaag ggaaagaaag tgatgaaaaa
    13801 agttttatta gtggtaaacc ccagttcagg cggtgaacaa gcaaaggaat ttgagcaatt
    13861 agcgatagca aaattggaat cggtgtttga tgaagtggtc gtcttgcata caaaaaaagc
    13921 aggggatgca aaaaacttta ctcgcgaagc ggctacggaa gggtatcata gtgtgtttgt
    13981 catgggcgga gatggaacag tcaacgaagg aatcagtgga atcgctgaac aagaacatcg
    14041 gccaaatttc ggatttttcc cattgggtac ggtgaatgac ttggctcgag cattagggat
    14101 accgctagaa cctgaggaag cgatcaatca tttttcgatt gaatcagtca agccattgga
    14161 tattggaaaa ataaatgacg actattttat gaatgtcgtc gcaattggct ctatccctga
    14221 atcaatcaat gatgtagatg cggagaagaa gacgaaattt ggaaaactag cttatttcat
    14281 gtcaggaatc aaacagttag cttccacaca atcctactcg tttcacgtag aagttgatgg
    14341 aaagaaggaa gaaattgaga gcagtacctt gctgatagga ctaactaact cagtcggcgg
    14401 ttttgaaaca ttacttccaa atgcaaaagt ggatgatggt aagctccatt tggtttattt
    14461 aaaagattct tctttgatcg atacgcttaa aacattgcca gacctattaa aaggagtaga
    14521 tgaatctacg aaaaatctag cctaccttac ttgtgagcaa ttagttgtgt cattagctga
    14581 tgaggaagaa ttgacgacga atgttgatgg agatgaaggg gataaattac ctgtgacaat
    14641 caaagtattg ccctcacatt tacaggttta ttgttaaaat ggaggttgtg accgaggagt
    14701 tggcgggaga acaccgcttg ttcgggataa gggactgtga caagactttt gtcacagcct
    14761 tttttttgta caaaaaagaa atttacacct aatagaaaaa tttttccata aaaatacata
    14821 ttttaaatat gaaagtacaa ttggaaatag attaaaacga taaaataaaa ttgtcaaaac
    14881 gtcagagagg atggtggaga atgaatcaat gtgtaaaaaa gctgattgcg tcgattttgt
    14941 ttgtcttccc tctcttgatg gtgacatccg tttttgccga tgtagagaaa acaaacgaat
    15001 caacgcaatc agagaatcaa agttttgaca agactacttt ttcagaagaa tcgattactt
    15061 ctgaaactac agaatctcaa agtgaggctt ctactaataa aaggcaagaa gaaaataaga
    15121 caagtgagac ctcagaagta gctgaacaaa cgtctgatag cagtgaagcg gcactggcaa
    15181 gagctgtttc agcggatggg tacagcgaag ctgccacaac agaagaactg gcgcagcttc
    15241 tcgcagacga atcagtaact aagatccgac tgattcagcc tttgacttta gacagggaat
    15301 tagagatcaa acgagatatc gttattgatt ttggtggatt cgctcacaat ttcggcacac
    15361 accatattta tatcaatgag atacctcagt cgatcgagtt ccagaatttt aaaggaacag
    15421 ctgcacatcc aggtggattg acaccaaata tcgatggaaa tgccattatc attgcttata
    15481 tgaccgattt ttggggaagc agatatcatt ttacgggaga aataaaattt accgggacac
    15541 ttgatttgta tgatgggtcc aaattaggac tgatttacgc tcctcgtgca actgtcacat
    15601 tagatggtgt ttccggtgtg ttggatgtcc aaccagtgaa ggaaggactg aatgccgctc
    15661 cgggtaaagc ctatttttgt agaagctatc aattgaatgt gatcaatggt agtcaacttt
    15721 atggaccgta tctagggaaa ttctacggtt ttctagattc aggagatgag actggtagtg
    15781 gaaaaacagc gccaggaatc catatcttaa gcggttcaaa ggtctctctt gattatgatc
    15841 gggcagatgg cgttagcgat gaaggagagg cagtcgatac gctacctggt aatgtcatgt
    15901 ttgaagtcag tggttcggga agcgaatttt cggtaaatac aaagatcaat attaccaatg
    15961 acaacaatcg cggaatcatc caaatgagag gttcaggatc gaaagtcact gtatcgaatg
    16021 gtgggaaaat caccatcaat acagcaacta cgggcggatt tcgcttgcaa ggagattcta
    16081 gtcaaatcca tgtatcaagt ggtggacaaa tctcggtcaa aatggctgga gatgacgatc
    16141 aacccgggaa caatgggatg cgttttgtag gaaaaggcct ttctttgatc gtagaaggag
    16201 ccggatcagt tatcgacatc gataagcaga gtgggcgttc cagcgcagtt cgttttgaga
    16261 atggtacgca aaagctgaca gtaaccaatg gaggctcatt gagggtaaga aatacaggtg
    16321 atggcaaatc ttacgtgaac agactcaatc aaggaaatca agcgatccaa ttttatgacg
    16381 cttctggttt tctagaaagt ccaggttcag ctgattttac agtcactgga gaaaaatcaa
    16441 atatcgatat acgcgcagat tccggagcaa ctgtagatac aactggagat attgatttaa
    16501 attttacggc aggtgaaaaa acttatttag tcatggtcgg gaaaacaggg gaagataaga
    16561 ccggcatcat cagtggggat gtgttgaatg tggacttaga atctcctacc tattttgatt
    16621 tccaaaacaa acggattgga agcgagacca atggcatagg cggctgggta ttccaaggaa
    16681 atggagattc ttctttaaag atcgcccatt cagaaatcgc tttatggcga aaaacaggcg
    16741 caacattcga taactttgaa aaagacccgg attatttttc tgatatgaca gacttatcga
    16801 tcaatgggac cgacttaggc aatttcgtat caagccctag tgcaggttta acaaatgaat
    16861 ttgctcagac aggtgccgga atgaaaaatt ataatcgaat cagtgccaat aatcagctcc
    16921 cgatgattga atccttaaga gtgccaacga atgcagataa aaaaatctat gggcatgtcg
    16981 ttgttcccga aggtgtcgag gcaaagccaa gagatgcttg gacaggcgaa gtagaagtga
    17041 ctttacgtat tacttacgca gatacgacga aatcatctgt agaaattact gctctcacgc
    17101 aaggacaaac agatgaaaat gaaggggcag gatataaccc atggggagaa ggcgaacaag
    17161 gcggcttgtt cgaagttcaa gtacccaatg atgagagatt aaaaacggag gatcaagtgc
    17221 aagttgttgc tgctaaaaaa gtaagaggtg gccaagaagt gacagaactg gactctgcca
    17281 aaacgacagt cgatgtgatt cctcctgaac ctgctaattt aagtaccaca ttgatcaatg
    17341 cagcttctcg cacgatcaca ggtacaggtg aagcaggagc cttggtttca ttgaaaaaaa
    17401 atcaagctgt tttgtcagaa acaactattg atgatacagg ggaatttgtt ctttctatcc
    17461 cagaaaatca actttttgca ggagatgtat tagaaattgt attaaacgac caagcaggag
    17521 aagctactgc taagggtgtg cagaacaagc caagtaccaa cgatgaaatt gggaatcaca
    17581 atccaagtga tacgccagtt gtttatcatg atgccccgcc ttttgcacct gctacgaaga
    17641 tcgtagtaga aggtggtctc tcatttcttg ccccttccaa actcgatttt ggtcagatca
    17701 aagcaactgg tttgacacaa gaagcatttg gccaaatgga tatacaggaa aaattatgga
    17761 tttatgatca gcgagaaacg aaaaatccgt ggatcttaac gataaaatta tctcagcctt
    17821 tcacaaatga tgcaggattt tctatgcagg atgtcctata tttcaagaaa ggagaggagt
    17881 atcaattagt gaatgaagaa gaactggaaa ttgtcagagg acaaaattca acgaatgtgc
    17941 aagcagatta ttcagaatat ttaaataacg gtcgggcgtt taaacttgtc ttaaccaaag
    18001 aaaatcagat agctggtaat tactcagcag agataacatg gacattagca gatgcgccga
    18061 cagaataaag atcaaggagg atgattgatg aatcgaatat ggaataaaag tcatcgaaac
    18121 aactgctctt tacttatgat ccttctcctt ggattcatcc tttggcagac acctgtccat
    18181 gcctccgaat tcaattttgc ggtagatttt gaattaccgg ctaatcagat tgataagaac
    18241 aaaacatata taaatgtaag gatggagcct gggaaaacac aggaaatccc ctacacggtc
    18301 tttaaccgaa cagataaatc agtaacagtt gagacattga tccactccgc tacgacgaat
    18361 tcaaacggtg tgatcgaata tggagaatca aaaagtaaat ttgatgaaac attaagttat
    18421 gatatgaaaa acttagcaaa aacagtggga gaaataactg ttcctcctca aagtgcggtc
    18481 actgagacac tttcactgaa agcgccagaa aaagagctgg ctggtgtggt cgcaggaggg
    18541 atcacgtttc aagaaaaaaa agagaacgtt tctaacaaaa gtacagttgg tgtcgtcaat
    18601 cagttttctc aagtgaaagc aatcttgatt tgaaatagcg aaaaagagtc ttccccagat
    18661 ttgaaattaa aaaaagctta tgcggatcaa agcgatggaa gaaatgtgat taaggcgaat
    18721 cttcaaaatc caaatgcttc ttatatgttc ggcttgacta catcggttga aatctacaaa
    18781 aaagaaacaa aagagcctta cctttcaata aaaaaagaaa aaatagatgt agcacccaat
    18841 acaaattttg atttacttgt cccattagaa ggaaaaaaat tagctccggg acattatcaa
    18901 gcggttgtga agggtgcttg gaaagaaaaa cgctggaatc tccataccga ttttgaaata
    18961 actgatgatg cagcggacca attgaatgaa aaggatattg atttaaaaaa tcagccagaa
    19021 acatttgact ggcgatggct gatacttgtt ggagtcattc ttattggctt atttgctgga
    19081 ttatttattt taaaagaaag aaggaaaaag aaatgaaaaa aacaattgca ttaagtaccg
    19141 ttctagctgg aagtctatta ttatgtacac aaagtgtttt tgctgagaat aagagctcag
    19201 ccactacaga aggaacgatt tctgtgacca aaccagatgc tgcaaatcct gaagacgttg
    19261 ttccagttcc tcctgttgat ccaaatgatc ctgatactcc ttttacacct gacgaaccaa
    19321 atcagggaac atccggatta ctgacgattg accaagctcc agatcttgat tttggaacaa
    19381 tcaagttagg tacagcaaaa acagtatatg ctgcattaca aagcggaacg gacagtactg
    19441 gagcacaaac acaagtacag aatttcgtac aagtaacgga taaaagcgga aattatgccg
    19501 gttggaaatt atcagtcaaa cgaacagaat ttattggtac aacatctgat acgaataaac
    19561 taactggttc aaaggtgaaa ttcaaaaacg ccgtgattcg aaatagcgca actaatagca
    19621 gtggaatgcc atctaccgtt tctggaactg gcgctgaagg aatcgaaatc ccagcagatc
    19681 aggcagtaga tctagtaact gcaggagcaa atgaagggat tggcacatgg atttattcat
    19741 taggagagaa tgtggaacaa gggagacaat cagtggaatt atttgttcct gtatccaatt
    19801 atgctgtaga caactacgtt tcaacattca catggtcgtt aacagatgct cctacagaag
    19861 aggaagcgta aaatcatatc aacgatttca gggagtggag ccgattcaag gcccactccc
    19921 tgaaatctgc tatgcttacg tctcagctct tattcatcaa ctcgaagcag ttttttggaa
    19981 gtcatggaaa cgttcctttg tcttttcaaa gatctttcga ccaacgagta aggagtcacc
    20041 gttgaaaaat tctaacacat aatgagcaga atcaattttc tgcaattgcg ataagttgat
    20101 acagtaagat tttaaaagta aaaagtaagc atacttttct aactctgtct tcaattgttt
    20161 taaagaagta ttcactaatt tatcttctgt agttgttttg accataactt ggtttttcaa
    20221 acctttgata ctttcaatat ataaaatttc ccgaggatac aggacgagac ttccaccttt
    20281 ttgatcaata aagattttat cgacatttac gatctgctgt tctttccaat tgatctgttc
    20341 tagcacttga cgaatttgtt cagataaatt cctgtctttt ctgatatatc caatcggaag
    20401 gattttccga ttgatgatct ctactgcaaa attcgtataa gcggtgaaaa aaacaatatt
    20461 actatgtgca ttttttttac gtatttgttc ggcgacatca atacctgtga gtgattgctt
    20521 caagtcgata tcgataaaga aaacatcgtg attatcaata gtgcccaact ggatcaactg
    20581 attgatttga gagatagaag tgagctgcac gatattctcg atatgatgat tgaccaattc
    20641 tctgatgatc cattcgctgt gaaactggtt atcttctatg ataaaatact ttttcatatg
    20701 caggctccta ttaaaaaaat atgtgtacat tttgaaacaa aacaatggag gaataagatg
    20761 gtcgcaacag tgacacttta tttgaccttt actttgattt tagatttagg tatctattta
    20821 ttcttttcga aacaactgct agtaaaaaac agctttggtt gtatagcttt gatggtggtg
    20881 acacttttac ttcctatccc taaagtgagc tttccttacc tagttggctt attattgatg
    20941 tttctttttt attttactac gaaaaacctg tttatcagct gtttgatcag taatcttttt
    21001 tttgctatca ctggactcag cttttttctg gtttttgatc tgatggatta tttaggaaag
    21061 agacatcttg gaacagcaag tttttttgca ctacttatcg catttatttt ctatctatta
    21121 gtaaaaagta tagacaaaca gacgagattt ctcaacttgc tttttactta tccaactgaa
    21181 cactattttg gaggtatttg tattttatta agctggggcg tgatttactt ggtattggga
    21241 agtgttcact tccgctcatc taattatctg cttctatctc ttttgtcgat agtcatcagt
    21301 tttttttctt tgtttttttc cattatgttg atcagtttcc aagtaaaaga aagacaacat
    21361 caggaatcgc ttcaactgta tcaaataaat gaagaatatt accatcatct agaggaattc
    21421 aaacatgatt ataaaagtct actgttttca ttaaaaatta ctcttcagga aaataaaaaa
    21481 gaaacgttgg aatttttaga aatattagaa gatcattctg cagctatttt tgaaaaaccg
    21541 caaatacaac agctaacaaa tattgtatcc ccagctgtca ggggtgtctt tttaaaattt
    21601 ctagaaaaag cagaatcgga gaaaatcccg atccaagtag ttattcctgt cgaagtgaaa
    21661 acaatcccga ttgatctggt tgtttttatt cgctgtttat ctatttttat ttctaacgca
    21721 ttcgatcatc gtgtagccga tcagagtccg attacgatca atctgctgga agaaaaagag
    21781 ggatcacgtt ttagtatagc gaacaaggca aatccaacga atttgacgct atcggagatg
    21841 gtcaaaagag gaaaaacctc taaaaaaaat ggcgggttag gtctatacag tgcgaaaaaa
    21901 atgttggatg tttacgaaaa tgcagaacta aaaatcgaat tttcaaaaca gaatcactat
    21961 ttttttgtag aaatgtatct ggcatctaga aagagcaaat cagcctactg acttactgtc
    22021 aaaggataag tttgaggaaa gtctacacgg agatagagac aacaacacag catattcaaa
    22081 agaaacagaa gcgtgatgac cgttgtcacc cttctgtttc ttttgctatg tagttaaaca
    22141 aagtagttga cacttccgct acttagtatt agtacgaaag tatacttcat gatactaagt
    22201 agataagaag tgaaaagcgg aacccacgat cgtcaaaatg ctaactgctt tgcttacacc
    22261 agataaaggg aagagcaata tcgcttattt taggcgtgga gaagcaagca aatccctacg
    22321 atcacaaaaa aaatgctgac ccagcagtta agagagttag aacaagatga aagtatccat
    22381 cgaaaagcct ataaccaagt cccgcctaaa gtagagtact cgctcaccga agaaggaaaa
    22441 tcattaagag agattctagt agcgttgtct gtttgggaag aagaacgtat cgaaaaacaa
    22501 aaagcctcaa gggaagaaga cacgctactg catacacatg gtggctacct aaattattga
    22561 ttttctatga catttatttt aaataaaaga aaacaggagt ttttttaaaa gcatcatcta
    22621 ttttttatgt aaaaaaatgg tatgattaat caagtaagaa gtgattggaa gaaggcaagc
    22681 aaatgaatag tatggaaaaa gtcaaccagt ttagagatga acgtaattgg cgacaatttc
    22741 ataacgaaaa agatttggct atctctattt ctttggaagc atctgaattg ttagaacttt
    22801 ttcaatggaa aacgccggaa gaagtaaaag aaacacaatt agaacgaatc aaagaagaac
    22861 tggcagatgt attgatctat tcatacatga tggcagacaa ccttcacctg gatctagatg
    22921 agatcattga agaaaaattg atcaaaaata atttgaaata tcctgtagaa aaaagcgcag
    22981 gtgtacgaaa aaaatacaca gagttgtaga aatgtgtcat tcggctgttg gaaagagcaa
    23041 aagaaaaaca gtaaaatgag tctggaggga gagaaaatga aaaaaaaagg aaaagcaaaa
    23101 agatggctag tcaatatcct cttgttcctc ttgttattag taggactagc tttgattttc
    23161 aatgaacaga tcaaggatta ttttgtcaga gagacaggag ataaatatgc tatagcgaat
    23221 gtaacaaaag aagatctgaa aaagaataac gataaagatg tcagttttga ctttgatgcg
    23281 gtagaaccaa tgacgacaga aggggtcatg aggtcgcaaa tgagaggaac agatctacca
    23341 gtgattgcta gtatcgcagt cccttcagtt tcaatcaatt taccggtttt caaaggcttg
    23401 gataatacgt cactacttta cggagcagga acattatctc cagatcaaga aatgggaaaa
    23461 ggaaattatg ccttagcaag ccatcgtgca acgaaccctg aattgctgtt tacaccgcta
    23521 gaaaatttag agatgggtgc caagatctat ttgacagatt tagaaaatgt gtatacatac
    23581 aaaacatttt ttaaagaaaa agttgctcct acagataccc aattactgaa tgaagtcgag
    23641 ggaaaagaaa ttgtcacact aattacttgt ggcgacatgg acgccgtcac gcgactagtt
    23701 gtacaaggag aactggaaag tgttacatcg ataaaagatg cgacagatga tatgagatct
    23761 gctttcaatc tagagacaaa aacattttag gatactggga cataagtcgt tacagattaa
    23821 aaacagccaa aggatatgaa tcgataagga aaaaccaatt tgctgaaaaa aagagaaacg
    23881 caaagaggtg catctctgat ttggttgttt tctagataga cttgtgttcc agttttttta
    23941 ttttttatat gaaaaggctt gcataaataa aaattcgatg gtatgatgat tttgaataag
    24001 aaatggattg ttactgtacg gcaggcaaaa ccagaattca gctaagaaac agtaggcgaa
    24061 ttctaggttt ttttattttt cttgagaggg cgaagcaaaa tgaaaattga taaaattttg
    24121 aataacaatg tcgtcatttc gaaaaatgga ttcggggaag aagtcgtttg tatgggaaaa
    24181 ggactagctt ttcaaaaaaa gattggggac gagatttcgc cagaagcggt ccaaaaagaa
    24241 tttgttttaa gagattcttt tgctaaaaac caattccagc agctgatggc tgatattccg
    24301 gcagaggaga tcgaatgggt caaacaagta gtcgaactgg ctgaagataa actaaaagtt
    24361 gaattttcgc ctaatattta tctgacattg accgatcatc ttcattatgc gatcacacga
    24421 gcaaaagaaa atatccaact gcctaatcca ttattatttg aaacaaaaaa attttatccg
    24481 aaagaatatc aagcagcgaa agaggctctt ttgttcgtga aacaaaagac gggtgtggaa
    24541 ctttctgagg atgaagccgg ttttattgcc tttcattttg tgaatagcca acaaggaaac
    24601 gaagacatgc aagtgacgat gaccgctacc acaatggtaa gagatatact gagtatcatc
    24661 agtaaattct tcggcatcgt gttcgatgaa gaatctttga attatcagcg tatcattact
    24721 catctgcagt tttttgccca gcgttatctc aaaggggaag gatctgacga gcaagatgaa
    24781 tttctctacg cattagttca aggaaagtat ccaaaagcgt ttcattgtgt cgaacggatc
    24841 aatgagtatc ttcgacaaac gcaaaaacag gaaatgggga tcgcagagca gatttattta
    24901 acgattcata ttcaaagagt agtaagtgag aaaaaaagca tccaatcata aggattgtta
    24961 ctgtatggca ggcaaaacct ggattgaaaa aataggttca cgaatgtttg tgactgtttt
    25021 tttcaatctg ggtttttatt ttttaaataa aaaagaaaga aggaaaaaac aatggataat
    25081 caagcagttg gtcgtcgtgt atgggaagcg gtaggaggac aaaagaatgt caaaagttta
    25141 gtacattgtg ctacgcggtt gcgattcaga ttaaaagatg aatctttagc agatacgcaa
    25201 aagttaaaag aagacccgga cgtgatccaa gtcgtacaaa gtggcggtca gtatcaagta
    25261 gtgattggca gtaatgtagc agatgtttat caatcgatcg tggatgagga aggtcttgca
    25321 gtttcggagg atggaaaaga agagtccaaa aatattttga atcgtctgat cgatattatc
    25381 tctagtatat ttacaccttt tctaggggcg atggcagccg ccggggtatt gaaaggattc
    25441 ttatcattgg caacggtgat gggctggctg acagctgata gcggtactta tcaaatattg
    25501 tttgctgcag cagatggtgt ctttaccttc ttacctgtca tgcttgcttt tacagcagct
    25561 aaaaagttca aagccaacca gtttttagca gtagcaattg ctatggcact tgtgtatcca
    25621 gctatcacag cagttgctgg tgcgggagaa gctatcagtt tctttgggat cccagtgatt
    25681 ctttcaccat ctggttatac ttcttctgtc ataccaatca ttctagccgt atgggttcaa
    25741 agtaaattag agccattcgt aaaaaaagtg attccgcaat ttctgcaaat gattttagtt
    25801 ccgcttgtcg ttttagtagt gatggttcca ttaacatttc ttgctttagg accaatcggg
    25861 acggttgccg gtaatgcttt aggcggtctc ttcaactcga tttatggatt tagtccaatc
    25921 gtagcaggtc taatcatggg aagtttatgg caagtattcg tgatgttcgg catgcattgg
    25981 ggattcgtac cgatcatgtt tttgaatatc gaacaatatg ggtttgatgt attgatgcca
    26041 atgcttcttc cagccatctt ggcacaaggt ggggctgctt tagctgtagc attgcgaaca
    26101 aaagatacga aacttcgagc attagggatt tcttctacag tgacttcgtt atttggaatc
    26161 actgaaccaa ctgtgtatgg tgtgacatta cctttgaaga aaccattcat tgccgcttgt
    26221 atttctggag gtattggtgg agctattatc ggattttccg gagtaaaagc tttctccagc
    26281 agtttggtca gcttattgac gattccgact ttcattaata cggtggatgg tgtagagtca
    26341 aatgtgacag tagcagtaat agctacagga attgcttttg ttcttgcatt cgtaggtaca
    26401 ttgattttag gatttgacga acaaacacaa gacaatcaat tagaaaacaa acatgcgaat
    26461 gcaggaaaac cgattacttc agcacggcat accttaaaaa gtcctttaac gggtaaagta
    26521 ctgccattga gtgaagtacc agaccaagtt ttttcttctg gcgtgatggg aaaaggtatc
    26581 gctattgatc cagaagtagg agaactagtt gcacctgcag acggagagat cacgactatt
    26641 tttccaactg gacatgctgt agggatcaca acgactgacg gagcggagat tttgattcat
    26701 atcgggatgg atacagtaga attaaatggg aatggctttg agatcttagt caaacaaggg
    26761 gatctcgtca aagcaggaga tttattgatt cgttttgaca tcgaagcaat tagagcagct
    26821 ggctatagcg taatcactcc agttgtcatt acgaatactg atgcatttgc cgatatactt
    26881 gaattagacc aaaaagagat tattgcaaac gaagatgttc tggcaattgt aaaataagga
    26941 ggaaatcagg atgcaatcac gtttttcaga aaaattttta tggggtggag caactgctgc
    27001 caaatcaatt agaaggggct tatgatcgag acggaaaagg actatccgtt gcagatgcta
    27061 tgcctggagg taagcagcgc tttgcgatta tcggcagtga agaatttgac tggacgattg
    27121 atcaagaaaa atatatttac ccgaatcatc gaggaatcga tcattacgat cgtttcaaag
    27181 aagatcttgc cttgtttgcg gaaatgggat tcaaatgtta tcgtttttcg attgcctgga
    27241 cacgaatttt tccaaatgga gacgaaggta ctcctaacga agcgggatta gaattttatg
    27301 atcagctgat tgatgagtgc ttaaaatatg atattgaacc tgtgatcacg atttctcatt
    27361 acgaaatgcc tcttcatttg gcaaaagaat acggtggctg gaaaaatcga aaactgatcg
    27421 acttctatga acgatttgct cagacagtat tagagagata cagcagtaaa gtcaaatatt
    27481 ggatgacttt caatgaaatc aattcggcct ttcactttcc agctttaagt caaggattag
    27541 taaaaagcaa tggagcagga gagtaccaaa atattttcca agcatggcat aatcaattcg
    27601 ttgcaagtag caaagccgta aaaatcggcc acgaattgcg ttcggatatc caaatcggct
    27661 gcatgattat ctatgcaaca acttacagca tcgatgccaa tccagtcaat caggcagcaa
    27721 caatgatcca aaaccaagaa ttcaattttt tctgcacgga tgtccaagta cgtggagaat
    27781 accctgcata cacagcgcgt acccataaaa aatatggagt cgatccggaa aaattagagc
    27841 aaacggaaaa agacttccag ttgctaaaag aatatccagt ggactacatc ggatttagtt
    27901 attatatgtc tacggcaatc aatgagactg atccacaagc agcaacttca gaagggaact
    27961 tgctgggagg agtaaaaaat ccgtttcttg aggcaagtga atggggctgg cagatcgatc
    28021 cagaagggct gagaatcgca ttgaatgaat tatacaatcg ttaccagaaa ccattattca
    28081 ttgttgaaaa cggtcttgga gccattgatc atatggaaga ggatggcacg attattgatg
    28141 attatcggat cgattattta cgtcgtcata tcgaagcgat ggctaatgca gtagcagacg
    28201 gtgtagattt gatgggctat acgccttggg gatgtatcga tcttgtcagt gcatcaacag
    28261 gagaaatgag taaacgttac ggctttatct atgtggatct agacgatgaa ggaaatggga
    28321 cactgaatcg atcaaagaaa aaatcttttg attggtataa agaagtaatt gcctcaaatg
    28381 gcgtaaactt gtaattaaag cagctgaatg aaaaagaaca cacttctcac atgtgatttg
    28441 tgttcttttt ttatttttta tgtgctattc ttttagtaat agtaaagcaa agggtgacgt
    28501 tatgaaaagg acacgtgtac tatacttgga agtcgcagat aaaattaaag aagatatttt
    28561 ctcaggaaaa tatccagtcg gttcgatgtt gccgacagaa agtgaattag aagaactgtt
    28621 tcaagtcagc aaaatcactg tccgaaaggc aatcgaactg ctggcatcag atgaatatgt
    28681 cgcaaagaaa agtggtcgtg ggacgacggt tctaagcaat cgtccttaca atcgattatc
    28741 caaagcggca agctttacgc aaatcttgga gaactcccga tatgaagtcc ggaaagaaac
    28801 gctggatttg caattggtcc atataaagaa ggaagatcct ttgttttcct ttttcggtga
    28861 gcaagctgtt tgttttaagc gcttatatta tttgaacaat cagccttata tttattttgt
    28921 tcattatctt ccacaagaaa tgatgcaatt cagcagagaa gaatttggca aagaatcttt
    28981 gtatcgtttg ctgacgaaaa aaggatatac tattgactca ttttctgatg atttctcggc
    29041 agtattttta acagaagaag aacaaggaat tctgaaaaca gacgcacaga tcggcatcaa
    29101 acggatacgg aaatcgtcta cgcaaattgg ggaaatgatt gaatattcgg aggcggtcta
    29161 ccatacggag ctgcatcctt accatattga ttatgaaaca taaataaaaa aagaggttgt
    29221 gaaaaagctg ccctgcatca agtaataaga acagccaaac aaaaatagct tctcatattt
    29281 ttcgtttggt taggcttatt accgcagata cacggctgtt tctggattac catttattct
    29341 ggataagggg gtgtggcacg attttcagaa ggaatctatg aaccaatcac tgtattgatt
    29401 ttagcttcga tggtaacccc attcggcttg acgatcgctt atttcttagg gaaaatcatt
    29461 cgtaaaaata tactcaatcg tcaagaaatc gatacattga agaccgcttt tcctatgggg
    29521 atctgtcaga tcacagaggg atgtttccct atcgtattga acgacttggc acgcaatgtc
    29581 atagcaacgg gagtcggtgg gacagtaggt ggcggattaa gcatgttttg gggcgcagat
    29641 agccgtattc cggcatcggg aatgtttgca gtcgctacga tgaccagacc atgggcattc
    29701 attggtgcat tgttagctgg ttcttttgtc acaggaataa ccattttatt attgaaaaaa
    29761 ccagtagatc cgaatgcaga aatcatccaa gaagaaaaag aggaagaaga catatcttgg
    29821 gatgatttga caattagtta ggagaggcag aatggattac aagaaaaaag tacaatagat
    29881 gtaccttgag acaggaatgg tatgaaagaa tcagagttag aaagaatcga atatacagat
    29941 tttggtttag gagcgatcga aactgtccag agcatcggca ccctccttga aatgacgatg
    30001 aaggaaaaat gagaaccttt cgttgtcgaa agggtctggt ataccttatg tcgaaggaaa
    30061 ggaaacaaaa aaactttcct gtactattcc aaaaaggaaa gaaagttatt atacagtaaa
    30121 aaaagaaatt attcttcatg ctagagatca gtacacgttc gagcccaata tcaaacacta
    30181 gttccaggca ggcgcaacag gtgcggttat cactgaattt tcttccagca gtgacgattc
    30241 aagtgatatt tttacaaatc cagctattta tcgttaacaa aaagattttt aggagaagct
    30301 gatgaacaaa aatgatcaaa ttttgatcgt ttagttcgtc ggctttttta tttagagaaa
    30361 catcatttat cagtgaaaca taacacgaga acaaaatgat tttttcgtta ataaaagaaa
    30421 caagtatttg ttgtttaaaa taatacacgt tattggcata taggacgaaa tcccagtatc
    30481 ttctataagt aagtcatttc atatcgatga aaacacgtta tttatttttg ttttatctat
    30541 aaaatggctg aaaaatgatt tttttctaaa tactatatat agtggcgtct atcctatata
    30601 ccactaaaaa actacaaaat ctagtctttt attttaaaaa aacacgtgta gaatgaagat
    30661 agacttttga gttagggagt gtttttttat gaaacttact gttttttgcg gttcccgttt
    30721 tggtaataaa gaaagttaca aatttattgc gcaaacgtta ggaaaataca tggcacaaga
    30781 agaaatcgaa ctggtttacg gtggttcata ctccgggatt atgggagtga ttagtaaaac
    30841 agtactggaa aataacggaa aagtcacagg tatctatccg aatgggcttt ttgaaactga
    30901 attgcctgga aaagatgtta ctacatttat tcctacagaa acgatcgatg aaagaaaggt
    30961 attattattt gaaaagggag atgctgtcct cgttttccct ggaggtctag gtacattgga
    31021 ggaattctca caacttcttt cttggatagc aatcggtctg acgcccgaca agccaatcgg
    31081 aatattgaat attggcggtt attacagcgg cttgcaacaa ttattagaaa cttttgcaaa
    31141 agaggatttt atggataaaa aatggttgga tcaagtaatc ttttccaaca atccattgga
    31201 actggtcaat gaattacgag cagtatctat taaaaatcag ttattattga aggaggcaaa
    31261 ataatgagtc aaatatattt agcaggacca tttttttcag aggagcaaat cgatcgagta
    31321 agtcgtatcg aaaaagcgtt ggaggaaaac aaaacagtca caagtttcta ctctccacgt
    31381 catcaccaag aaagcaatta tgaactattc agcgctggat gggcgcagga agtatacgaa
    31441 aaagatatgg aagaactgac aaatgcagaa tttgttgttg ctatccttga ctttgagcat
    31501 caaacaatcg atccaggaac ggcttacgaa ttaggtgtgg caactatgtt gaaaaaaccg
    31561 atgatcattg tacaagaaga gacggtacct acgaacttga tgattacaca aagtctccat
    31621 acgtatttaa aatcagacca agctgtacgt gaatatgatt ttgaaacatt gcctgttgaa
    31681 acttacgtag gcgagtactt atagaagagg aagggttgta tgtcagtgat tttgatttca
    31741 ggaacgattg gtgcaggtaa gagcagtttg acggatatgc ttgcaaaaga gattgattct
    31801 aaaccatttt atgaaaatgt cgaagataac gaagtattgc cgctctttta ttcaaatcct
    31861 gagcagtacg cttttttatt gcaaatcttc tttttgaaca agcgattttt agcaatgaaa
    31921 aatgctttag tgaacgatga caatgtgcta gaccgatcca tctatgaaga tagcctgttg
    31981 ttccatctaa acgctgattt aggtcgggta acggatatcg aagtacagca gtacgatagc
    32041 ttattagata cgatgctaaa cgagttagat gatgttgctc caaaaaaacg gcctgatttg
    32101 atggtccata tcaaagtttc tttggacacg atgctagaac gtatcaaaaa acgtgggcgt
    32161 gactatgaac agctagaaag cgatgagacg ttatatacct attatgaaac gttgaacacc
    32221 cgctataatc aatggtatga agattttgat gtatgtccta aaatacaagt ggatggggat
    32281 aaattcgatt ttgtagaaaa tccagaagat gcaaaaaagg tgatccgtca gatccaagaa
    32341 aaattagctg aattggaagg aaagcctgct ggaaaatatt ttacggataa ccagcatgca
    32401 aaaggtcaag aattaggcta aaaataaaaa accaaacgac gggaaagaaa aattactacg
    32461 tgatgtagaa taagcctttc tatcgtttgg ttattttata ttcaaatatt tgaaataaat
    32521 aaatctttac tctttgattt gtaaactgaa atgatccact gtttgtttta ctgatgaagc
    32581 ttgatcaaag gaagaagctg agtttgaagt tcataaaaat cagccatagt gagttttgct
    32641 tggatttgtt tatcttcttc tactttttac ataattttga acaggataag attcaaatgt
    32701 tttaaatgaa tctttgacta gtttgtgaca gttgtcgcat atgctcaagc ttccttggtc
    32761 attaaattct gtatggatat ttttgttgtt attacatgta caataatcag gactatcgat
    32821 catccatttc gtctccttta caatcatctt gcttctttac tatagcagaa aagaagggga
    32881 gaaacaaaag aagatccaac agacggcact atagactgta gatgttgcca tcctcatcct
    32941 tcaaacggta attaccgatt tttgtaaata tgactgttgt ttctttttta ttttcttttc
    33001 ctgaaataat atattcgtca tctttggtag tgatcttgat atctgtcagc actgtttttt
    33061 cttcagatga ttcagaagaa tcagttcctt gtgtctgata cgtgcgtgtc cattccattt
    33121 ttttatctgt aaatgtcaac tgtcccgcgg ataaatccat atctttacgt acttttagcg
    33181 tatatacact gttggtggta tctgcttttt ccgcttttaa gacagaagaa tcggttgttt
    33241 cgtccgagga ttggtcagag ccgccaaaca aatcacagcc agtcaatgtc aaaagtcccg
    33301 cagacaaaat aaatagtacg attaatttct tcaaaatact tcccccattt gataggataa
    33361 agaaattata ccattggaat gtaaaacttc aaacggatgg aagagaaaaa cgcacaaaac
    33421 acccacaaat agagtaaatt ttagaataaa attttaaaaa ggcaaaaaat ccgccacttt
    33481 cagaaagcgt tttaaaaaag tgttgcaatc ttttaaaaaa atgctatatt aaaaatgaac
    33541 cggttcaaat acttgtttaa gtataattaa tagtgagagg aagttgctct actgaagact
    33601 ttagttatat ggatattgat gacaagcaac actatgcaaa gaatgcggtc atcataatcg
    33661 aacaacaaga aagagaaatc aaatgtaact ggcaggagtg agaatgtatg gcatcgatcc
    33721 gtgatgtggc taagatggct ggtgtttcag tgggaacagt ttcccgttac ttgaatggtc
    33781 agcaactgaa ggaaaagaac atgaaaaaaa tagcagaagc gatttcagcg ttggactata
    33841 aagaaaacat cattgctaaa gggttgaaga ataatcgaag cttctctatt ggacttttga
    33901 taaacgggat ttccagccgt tttgggtcgg aagttgtctc agggatcgaa agagtagctg
    33961 aagaaaatgg gtatagctta ttattaagcg ggtttgccga tcagccagaa aaaattgaac
    34021 aaaaaattga atacctgatg aagcatgtga tcgatgggct gattgtcttc ttatccgaag
    34081 aagagtggtc tggatttgaa atgctcacaa aaatgagtat tccagttatc gcattgaatt
    34141 gtccagaagg accagcaggt attgatacaa tattagtgaa tgatcgagaa agtgtagcaa
    34201 aagtgatcgc tcatcatggt gaacaaggac ataaaaaaat cggatttatt gctgccactc
    34261 aaacagacta cgtagcaaga gaacgtttag caggagccaa agaagcggtc caaaatgatc
    34321 catcgatcca attagaggtc tttacaggag actattctag aaaaagcggc tattacgggg
    34381 caaaagcatt attagaaaaa ggggcaactg ctatttttgt cagcaattac aatatgtcga
    34441 ttggtgccat cgaatgtttc aatcaagaag gtgtcagaat cgggaaggat attgcgttca
    34501 gtcactatga ttatttagat aaaaacgatc tgagtatcct tccgaaaatc acgatcacgc
    34561 caccaacaga taaaatcggg gaacgagcag cagaacagct gttaaaacga atcaaaaaag
    34621 aagaggaaga aaatgaaaaa gtgattgtga tggaaaatat catacatgga atcaactaaa
    34681 acgaactaaa aaagaaaaag aggtgctttg atgaaaaaat atcaatttcc agcaaatttt
    34741 tggtggggtt ctgcagcaag tggcccacaa acagaaggaa ggatttcagg cgatggaaaa
    34801 ggagagaaca tttttgatta ttggtacaaa aaagaaccgc aaaagttctt tgatcaagta
    34861 ggacctgaaa aaacctcgca agtttatacg aagtatcaag aggatgtaca gttgatgaaa
    34921 cagactgggc ataattcatt tcgaacatct atccaatgga gccgtttgat acctgatgaa
    34981 tctggaacaa tcaacgaaga agccgttcgt ttctatcatc gttattttga tgagttgatt
    35041 gaaaacggta tcgaaccctt tgtgaatctg tatcatttcg atatgccgat gtatctgcaa
    35101 gaaaaaggcg gatggttgaa ccgagaaacg gttgaagcct atgaaaaata tgccaaaagt
    35161 tgttttgaat tgtttggttc gaaagtaaaa aaatggttta ctcataatga accaatcgtt
    35221 ccagtcgaag caggttattt atatcgatgg cattatccag aagagtcaga tatgaaaaaa
    35281 gcgatccaag ttggctatca tgaagcgttg gcttcagcgt tagccatcaa atcttaccat
    35341 gaaatggagc aaggtggtgt tattggtatc atcttgaatc tgacaccaag ctatccaaga
    35401 gacgaatcaa accctgaaga tgttaaagca gcgcaaatag cagatgcgtt cttcaatcgt
    35461 tcattccttg atccagctgt aaaaggcgag tttccaagtg aattgattga gatagtaaaa
    35521 gaacttgatc tcatgcctgt gatccatgca gaggatttga cggttatcaa agaaaataca
    35581 gtagatcttt taggtatcaa ttattaccag ccacgccggg taaaagcaaa agaaacacca
    35641 atcgatacta aaaacggtcc aatgcctgag gattatttcg acaattatga aatgcctggt
    35701 cgtaaaatga atccttatcg cggatgggag atctatgaac aaggaatcta tgatattttg
    35761 atcaatgtac gagatcatta tggaaatatc gactgcttta tttctgaaaa cggtatgggt
    35821 gtagaaaacg aagcacgatt tatcaaagaa gatggcatga tccacgatga ttatcggatc
    35881 gaatttgtcc aatctcatct gaaatatgtc catcaagcca ttcaagaagg tgccaattgt
    35941 ttaggctatc atatgtggac atgtatggat aactggtcgt ggaccaatgc atataagaat
    36001 cgatatggct ttatttcagt agacttagca caagatggaa aaagaacaat caaaaaatcg
    36061 ggtcattggt tcaaagacgt atcagataaa aacggatttg aggcttaaga aagaatagtt
    36121 gagagcaaat gcgtgaaatt ctccattctt accattttgt gaaaaagaag ttcaattatt
    36181 tacgatcttt ttcaactttt ttgtctttct atttcatttt ttgtatataa tagaatagtc
    36241 aaaattaaat aggagtggag gaaagcatga ggaaaaaaag accgtttctt ctcttttggt
    36301 tattcggttt tttcttactg cttattttat ccgcagcatt ttcaggaaaa atgacagcag
    36361 atcttgctca gaaagaaaca gaaaaaatcg tagcaagcaa agaatcagta gataatttca
    36421 aaaaagaatt ctcaacttat tggtgggcgg agtataaaat aactgccaat tacgaaaaag
    36481 gcgaattgga catttatttg ccaaaaggaa aagaagaaaa tcaatttatt aatatcgatg
    36541 acgtacttag tgcgatttct ctcagacaat atcaatttaa agacaagcat ttgaccgttt
    36601 tgcttttgac tcatgatggc gacaaagtag cccgtttgaa aaatggaaaa gtgatcaccg
    36661 cttattctga aaaagtagac aaaaaagtgg tcgccgatcg tttagcggaa tggaaaaaga
    36721 caaatgaaca agtcaagaaa acaactgtct cttctcaaaa aaacgagtcg acaggaacaa
    36781 gtccttcaga ttctggcagt ggacaaacga taacgggaag tgaagaaaat caagcctcaa
    36841 ttattacgaa tgagtcatca caaggacaat atcaatccta tggacaatga aaaacagagg
    36901 ttgtgaaatc ag
//
LOCUS       DACDXO010000023        34636 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E23605_2017
            SAMN12639989-rid9881463.denovo.023, whole genome shotgun sequence.
ACCESSION   DACDXO010000023 DACDXO010000000
VERSION     DACDXO010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639989
            Sequence Read Archive: SRR10023389
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741914.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:42:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,972
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 33 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 17 of 73
            Pseudo Genes (multiple problems)  :: 10 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34636
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639989-rid9881463.denovo.023"
                     /isolate="E23605_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            591..1280
                     /locus_tag="IWM35_001312"
     CDS             591..1280
                     /locus_tag="IWM35_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9919172.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1267..2475
                     /locus_tag="IWM35_001313"
     CDS             1267..2475
                     /locus_tag="IWM35_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP9919173.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2769..4040
                     /gene="serS"
                     /locus_tag="IWM35_001314"
     CDS             2769..4040
                     /gene="serS"
                     /locus_tag="IWM35_001314"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HAP9919174.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
     gene            complement(4589..6073)
                     /gene="guaB"
                     /locus_tag="IWM35_001315"
     CDS             complement(4589..6073)
                     /gene="guaB"
                     /locus_tag="IWM35_001315"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HAP9919175.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
     gene            complement(6578..7273)
                     /locus_tag="IWM35_001316"
     CDS             complement(6578..7273)
                     /locus_tag="IWM35_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HAP9919176.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(7363..8463)
                     /gene="ychF"
                     /locus_tag="IWM35_001317"
     CDS             complement(7363..8463)
                     /gene="ychF"
                     /locus_tag="IWM35_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HAP9919177.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(8472..8663)
                     /locus_tag="IWM35_001318"
     CDS             complement(8472..8663)
                     /locus_tag="IWM35_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HAP9919178.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(8684..9574)
                     /locus_tag="IWM35_001319"
     CDS             complement(8684..9574)
                     /locus_tag="IWM35_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HAP9919179.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(9561..10328)
                     /locus_tag="IWM35_001320"
     CDS             complement(9561..10328)
                     /locus_tag="IWM35_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAP9919180.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(10340..11053)
                     /gene="rsmG"
                     /locus_tag="IWM35_001321"
     CDS             complement(10340..11053)
                     /gene="rsmG"
                     /locus_tag="IWM35_001321"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HAP9919181.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(11285..13186)
                     /gene="mnmG"
                     /locus_tag="IWM35_001322"
     CDS             complement(11285..13186)
                     /gene="mnmG"
                     /locus_tag="IWM35_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HAP9919182.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(13203..14600)
                     /gene="mnmE"
                     /locus_tag="IWM35_001323"
     CDS             complement(13203..14600)
                     /gene="mnmE"
                     /locus_tag="IWM35_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HAP9919183.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            14944..15282
                     /locus_tag="IWM35_001324"
     CDS             14944..15282
                     /locus_tag="IWM35_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9919184.1"
                     /translation="MSPFRSAALEEESDPRINDVVLSTGNINQKYIVRDIVFVVECLE
                     ADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQKKRDGKYFLELFAYGT
                     VIQFIQTTIG"
     gene            complement(15450..16208)
                     /locus_tag="IWM35_001325"
     CDS             complement(15450..16208)
                     /locus_tag="IWM35_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HAP9919185.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(16226..17044)
                     /locus_tag="IWM35_001326"
     CDS             complement(16226..17044)
                     /locus_tag="IWM35_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HAP9919186.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(17041..17397)
                     /gene="rnpA"
                     /locus_tag="IWM35_001327"
     CDS             complement(17041..17397)
                     /gene="rnpA"
                     /locus_tag="IWM35_001327"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HAP9919187.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(17515..17649)
                     /gene="rpmH"
                     /locus_tag="IWM35_001328"
     CDS             complement(17515..17649)
                     /gene="rpmH"
                     /locus_tag="IWM35_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HAP9919188.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            18172..19506
                     /gene="dnaA"
                     /locus_tag="IWM35_001329"
     CDS             18172..19506
                     /gene="dnaA"
                     /locus_tag="IWM35_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HAP9919189.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDLKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            19704..20834
                     /gene="dnaN"
                     /locus_tag="IWM35_001330"
     CDS             19704..20834
                     /gene="dnaN"
                     /locus_tag="IWM35_001330"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HAP9919190.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
     gene            21063..21305
                     /gene="yaaA"
                     /locus_tag="IWM35_001331"
     CDS             21063..21305
                     /gene="yaaA"
                     /locus_tag="IWM35_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HAP9919191.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            21292..22416
                     /gene="recF"
                     /locus_tag="IWM35_001332"
     CDS             21292..22416
                     /gene="recF"
                     /locus_tag="IWM35_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HAP9919192.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQTRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDNRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            22413..24359
                     /gene="gyrB"
                     /locus_tag="IWM35_001333"
     CDS             22413..24359
                     /gene="gyrB"
                     /locus_tag="IWM35_001333"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HAP9919193.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            24379..26850
                     /gene="gyrA"
                     /locus_tag="IWM35_001334"
     CDS             24379..26850
                     /gene="gyrA"
                     /locus_tag="IWM35_001334"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HAP9919194.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDYAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKEDQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            27086..27385
                     /locus_tag="IWM35_001335"
     CDS             27086..27385
                     /locus_tag="IWM35_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HAP9919195.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            27435..27947
                     /gene="ssb"
                     /locus_tag="IWM35_001336"
     CDS             27435..27947
                     /gene="ssb"
                     /locus_tag="IWM35_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAP9919196.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            27973..28209
                     /gene="rpsR"
                     /locus_tag="IWM35_001337"
     CDS             27973..28209
                     /gene="rpsR"
                     /locus_tag="IWM35_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HAP9919197.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            28352..30325
                     /locus_tag="IWM35_001338"
     CDS             28352..30325
                     /locus_tag="IWM35_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HAP9919198.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            30331..30783
                     /gene="rplI"
                     /locus_tag="IWM35_001339"
     CDS             30331..30783
                     /gene="rplI"
                     /locus_tag="IWM35_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HAP9919199.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            31057..32424
                     /gene="dnaB"
                     /locus_tag="IWM35_001340"
     CDS             31057..32424
                     /gene="dnaB"
                     /locus_tag="IWM35_001340"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HAP9919200.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
     gene            33204..34496
                     /locus_tag="IWM35_001341"
     CDS             33204..34496
                     /locus_tag="IWM35_001341"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HAP9919201.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
BASE COUNT        10554 a         6733 c         6844 g        10505 t
ORIGIN      
        1 ggagtgtttc ccgattcgtt tttggctgcc gtagcaactt ttatatcata tcagagagac
       61 aagtaattgt caactctttt ttaaaagttt tttcgatttc gtttttcgaa atcttccagc
      121 aactcattta tcataactta tcgtttgtta tttgtcaaga acttttttga agtttttttc
      181 gatagaatca agcttcgcaa gtggttcttt tttaacgact cagttatctt atcatgtcat
      241 ttccaacatg tcaacaactt attttttatt atttaataag ttattggtgt tcttttgaac
      301 aaataataat atatcaacat cacatgtgaa agtcaataag aaaatgctta tttatcaaca
      361 tttctagctt acatgatcat tggttagttc tggtcctttt tcgttcgtct ttataactcc
      421 gagagacgta aaatcctttt gttttcagtt taatgttcat gttcttgtct tattcacacc
      481 aactagatgt ctcaccttct aaaattttcc ttaacaaagt gctgctttta tctatttctg
      541 cttttttttg ctagaataat agtaacaata tgactctgga ggatttattt atgaaaattt
      601 tgattgctga cgatgataaa gaaatcgttg aattattaag tatctatgtt cacaatgagg
      661 ggtatgaagc agtaaaggct tatgatggga aagaagcatt atctaaaatc agaacaatcc
      721 ctgatattga attacttatt ttagatatca tgatgcctga gatggatggg atgcaagtag
      781 tgaaagagct acgaaaagaa tcccaaatac caatcattat gctgacagca aaaactactg
      841 atatggataa gatcaaagga ctggtcgctg gtgcagatga ttatgtgacc aagccattta
      901 atccgttaga agtaatggct agagtaaagt ctttattacg gcgtacaaaa atgcagattg
      961 ccccagatca gcctgatgta ttagaagtaa atgcattaat catcaataaa gactctcatg
     1021 aagttaaaac aatcgatggg aaagaaatcc agttaacagc tttggaattc ggtatcctct
     1081 acttgcttgc tagtcatcct aatcgagtat ttagcgcgga tgaaattttt gagcgtgttt
     1141 ggaaacaaga aagtattgtc tctgctaaaa ctgtcatggt tcacgttagt catttaagag
     1201 ataagattga agaagcaact ggtggtgaaa aagtcattca aaccgtttgg ggagtgggct
     1261 ataaaattga cgcaagatag taaacgagca actgaaaaac gccgtcggat caatttgact
     1321 tcaaaagaaa tcagcgaatt gttagcggaa ggaatcatca cgattatcct tttgcttcta
     1381 ttaaatgttt ctattctggt tgttatcagt tcggtgatca acagttcacc ttctttgacg
     1441 aatgcgattt gggattcaaa gaatattttt gcagaacgat tgaacactga tttgttctgg
     1501 aacggccgga attttattat tccctttttc tttctattag atattggtgt tttgtattgg
     1561 cgcttgattc gaagatatcg ccaaatgcaa ttgcggcata tcatcagcga acttcactac
     1621 atcgcaaacg gaaattatga tcatcgtatc ccttttgaac tgagtggtga cttaagccga
     1681 gtggtaacaa gtatcaatgg attggtggat agtaccgtag cagcgattga agacgaacgt
     1741 aaaatcgaaa aatcaaaaga tgaattgatc acgaatgtca gtcatgatat ccgtacccca
     1801 ctcacgtcca tcattggata cctaggactg atagaagatg gccagtatca ctcagaagaa
     1861 gatcttttaa agtatacgca tactgcttat atcaaagcaa agcagatgaa atctttagtg
     1921 gacgacttat ttgaatacac taaggtacgc cagcctgctg tcccggtgaa tttttctgcc
     1981 tttgacatga tccagttgat cgaacagtta gcagctgatt ttgagttgga agcttctaaa
     2041 aaaaatatcc aaatacttgt tcaatcaaaa gtagactccc ttatcatgga tggcgatacc
     2101 gaaaaacttg tacgtgtctt caataatctt ttgacgaatg cgttgaaata cggaaaagga
     2161 gcaacgaaga tcgtaatcga agtagaacgt atcggttcag aagttgttgc aacagtcaaa
     2221 aataacggag caatgatccc acaacaggca atcgataatc tattcgaccg tttttatcgt
     2281 gtagaagaat ctcgttcaca agcaactgga ggaactggac ttggtttagc aatcgctcaa
     2341 agcattgtgg ctttgcacgg cggatacatt tatgcaaaat cagataagca atggacttct
     2401 ttcatcatgc accttccaat caagaaaaat gaaaaattac ctattgatac acaggaagtt
     2461 attgacgaaa gctgaaatat tcgctattct tattacaatt attagaataa atgaaaaaca
     2521 cagaacccag tacactttat tctttttcag agagctgatg gcaggtgtaa atcagcaaag
     2581 gataatttgg aagttccagt tcgtttacgt gccctttcga aataagaaag gtcggttcaa
     2641 cagccgttat gtgttcaaca agtgcagctg acgctgaact tgggtggtac cgcgaatttc
     2701 agactgattc gtcccaagaa taatgggatg gatcagtctt tttttatttt caaaaaggag
     2761 gaaatcatat gttagatgta aaaatgatgc gacaaaatat tgaagaagta caagaaaaat
     2821 tgatgactcg gggcgtaaaa aaagaaacat tagctgaatt catggaacta gatgaaaatc
     2881 gtcgtaagct actagtcaaa agtgaagaat taaaaaaata tcgaaatgat gtttctgctg
     2941 agatcgccca attgaagcgt gcaaaagaag atgcttctgc taaaatcgct gaaatgaaag
     3001 aagtcggtgg caacatcaaa gcattggata ctgaaattgc tgaagtggat gaaaaattga
     3061 aacaaattgc aactactttg ccaaaccttc cccatgattc tgtgccagtt ggaacagatg
     3121 aagaggacaa tgtagaaatc cgtcgttgga gcgaaccacg tacatttgct tttgaaccaa
     3181 aaccacactg ggaaattgca gaaaatcttg atattttaga ttttgaacgt ggcgcaaaag
     3241 tagccggtag ccgttttgtc tattacaaag gattaggtgc tcgattagaa cgtgctgtct
     3301 acaacttcat gcttgatcag catatttacg aacacggcta tactgaaatg atcactcctt
     3361 atatcgtcaa cagtaaagcg atgtttggta cgggacaatt tccaaaattc aaagaagatg
     3421 ttttccaatt ggagaatact gatctaacat taatcccaac agctgaagta ccattaacta
     3481 actattataa tgatgaaatt ttagatggtg cagatttacc tatttatttc actgcactaa
     3541 gccctgcttt tcgttctgaa gccggaagtg ctggtcggga tacacgagga ctgattcgtc
     3601 ttcaccaatt caataaagta gaaatggtca aattttcgga tgtagagcat tcttacgaag
     3661 aattggaaaa aatgacagct gatgcagaag atatcttgaa aaaattgcat ttgccttatc
     3721 gtgtgatggc cttatcaact ggagacatgg gcttttctgc agctaaaact tatgacttag
     3781 aagtatggat acctgcacaa gatacttatc gtgaaatcag ttcttgttct aactgtgaag
     3841 atttccaagc acgccgcgca atgatccgtt atcgcgataa tgatggaaaa atccactata
     3901 ctcatactct gaatggttca ggacttgcag ttggccgtac agtagctgcc attttggaaa
     3961 actaccaaaa tgaagatggt tctgtcacag ttccagaagc gctcgttcct tatatgggga
     4021 atctgaaagt tattaaataa agaggctgtg aaaaagccgc ttagtttcga gtattaagaa
     4081 aaattttgtg aaaatggctt tccacatttt tgcataaatt ggacttaata ccgagaaact
     4141 ggcttttgaa caccatttat cgagaggttg tgaaatcagc gaattaacct gagtattaga
     4201 gaaaaaaatg gaaaaatagt ccctcatatt ttaccgtttg ttgaactaat accgagggtc
     4261 agctgattga acaccgttta gtggagaggt cgtaagccta ccccccagct cctagttaca
     4321 aggacaaatt caaaaaacag ctcttcatat tttatgaatg ttgtacttgt gattcggagg
     4381 tggcaaggga acactgttta ttcaaaaaga ggctgggaca gaagcgttta actccaagaa
     4441 ataagaagaa attcacgaaa attgcttttc aaatttttgt gaatttcttc ttatttccga
     4501 aggagttgct tctacttccg ccgtttatac gtgtttagag cgtgagacaa aagtgttctt
     4561 tacttttgtc ccacgctctt taatgttttt aagattcaac tgaatagttt ggtgcttctt
     4621 ttgtgatttg tacatcatga gggtgtgatt cttttaatcc actgccgctc atttggataa
     4681 attgcgcttc atcacgtaat tgctgtagat tggctgcccc aacataaccc attcctgaac
     4741 gcaaaccgcc gatcatttgg aaaataatat ctgcaacact tcctttataa gcaacccgac
     4801 cttcgattcc ttctggtact aatttatttg cttcattgac actaccttgg aagtaacggt
     4861 cttttgaacc tttttccatc gcgcctaatg agcccatacc tcggtatgtt ttgaatcgac
     4921 gtccttggaa gatttcgaat tcacctggtg attcatctgt ccctgccaac atacttccta
     4981 gcatcactgc atgtccgccg gcagctaatg ctttgacgat gtcaccagaa tatttgatcc
     5041 ctccgtctgc aataatcgct ttgccatatt gacgagcaac tgatgctgca tcataaatag
     5101 ctgtgagctg aggtacacct acaccggcta ccacacgtgt cgtacaaatc gatcctggtc
     5161 cgataccaac ttttacgaca tctacacctg catcatataa agcttttgtc gcttcagccg
     5221 tcgcaacgtt tcctgcaatc aatgttgcat ctgcaaaagt tgaacggatt tcttgaattt
     5281 ttcggatcac acctgcacta tgtccatgag ctgtatcgat gatgattgca tctgcccctg
     5341 cttctaataa agcattcgca cgctcaaatg tatcgcttgt tacaccaacg gctgctgcta
     5401 caagcaatcg accatgttca tcttttgctg catttggaaa ttcgatcact ttctcgatgt
     5461 ctttgatcgt gatcaatccg cttaaacgac cttcgttatc tacgattggt aatttttcga
     5521 ttttatgttt ttgcaaaatc ttttctgcat cttttagaga ggtacctact ggggcagtca
     5581 ctaagttgtc ttttgtcatc acatcagcaa tcggcatgtg ataatctgtt acaaaacgca
     5641 tgtcgcggtt tgtaattatc cctaccaatt ttctgttttc aagtgtttca acgattggca
     5701 caccactgat acgatatttg ctcatcaatt cttctgcgtc tgctacaaga tgctgtggcg
     5761 ttaagaagaa cggatcaatg attacgccac tttcagaacg tttgactttt cttacttcat
     5821 ccgcttgctg gctgatcgtc atgtttttat ggatcacacc tagtccgcct tgtcttgcca
     5881 tagcaatggc catcttgctg tcggtaacag tatccatact tgcactgatg attggaatat
     5941 tcaacttaat atttttagct aattgtacgc tcatatccac ttcgtttggt aacacgtgac
     6001 tttctgctgg tatcaataag acatcatcaa aagtaaatcc ttttttcgtg aactttgttt
     6061 cccagttgga catttttagt accactcctc taatttagtt tatctattaa aataattgat
     6121 tttttcattt aagtcaatga tctacttgga atcaagttag ctttctcata gaataccaat
     6181 ataaagcgtt tacatctgtg tatattaaaa gagattgtaa aaaagctgtc ctgcatcaag
     6241 taataagaac agccaatcaa aaacagcttc tcatgttttt gaattggttg ggtttattac
     6301 cgcagatgca aacttttgaa caccgtttag tggagaggct gtgaaaaagc tgcttagttt
     6361 cgagtagtaa gaaaaatttt gtgaaaatgg ctttccacat ttttgcataa attggactta
     6421 ataccgagaa actggctttt gaacaccgtt tatcgtaaaa agggactgtg acaagacttt
     6481 tgtcacagtc ccttactatt atttaactat ggataaacaa aatgaggttc tttactaccc
     6541 ctagatatct agcaaaatag ctgatctttt tgctagttta tcttcttggg acggataaac
     6601 ttccaataat cccgtatttt ttcttcaagt agtaacggat tgctaaagca gttgccccga
     6661 taatgataac tactactgga tctaatactg gattgataac tggtggcaat agtgttgtcg
     6721 cagaaaacag tacgacccaa agaagtgtcg ttccacctag gataagcaat gtcttaccca
     6781 ttcctggttt cttgcttcga tctgctccgg gatattcata ttgatagatg tatttgtaca
     6841 tcatataaaa gacccagcca ccgattgcag aacctagaac gagtgtagaa aagccataag
     6901 ctggcgttgt tccacgtgaa aacaatccgg taatcgcaat catgatactt aatagaccaa
     6961 gcaacagcaa cgtattatct aaccacatca ataaaggggt ggatgaattt tttacttctg
     7021 ctggcttatt gatgattgct tccgttcttt cagagactgt cccaaacaat tgacgggctg
     7081 ttcttccgcc tttttgttct gcaactaaag taggcagaat gtcatgaagg gcttttactt
     7141 tttcttcttc agacaaattc gctgcttcta aagatttttt caaatcaaaa atgtactgtt
     7201 gatttctttt ggttaatttt gtttctaaac tattgttttc ttttacgatt gctcgtaatt
     7261 gttctggttc cattatctaa tagtcctcct tagccgttcc ttttctcaac ggtcgtcaca
     7321 aaagacattc taccatattc tacagctagt ttgatagccc ttttacacat taaagcggaa
     7381 taacattacg tcgccgtctt gtacgacata atcttttcct tctaatcgaa cacgtccagc
     7441 ttcttttgct gcatgcatgt ttccgtaatg atccaagtct tcaaaagaga cagtttctgc
     7501 acggataaat ccgcgttcga aatctgaatg gatgattcct gctgcttgtg gtgctttgat
     7561 tccttttttg aatgtccaag cacgtacttc ttgttcccca gcagtaaagt aagtagctaa
     7621 gcctaacaaa tcataagcag cacggatcaa ctgatccaaa cctgattctt cgattcctaa
     7681 agcttctaaa aattccgctt tgtcttcttc atctaattct gcgatttctt cttctgcacg
     7741 agcactgacc acgatcactt ctgcattttc ttctgcagca aagttgcgca cttcttgtac
     7801 ataatgattc gcgtctgcat ctgctacgtc atcttctgag acattggcta cataaagaat
     7861 tggttttgtc gtcaataaaa atagtgattt aacgattttt tgttcttctt cagtaaattc
     7921 gatcgttcgg gcagattttc cttcttccag tacaggtttg attttatcca gtacagctag
     7981 ttctgccact gcatctttat ctttggtttt agcaactttt gctacacgag tatagcgttt
     8041 gttgatagat tctaagtctg ctaacactag ttccaagttg atcgtatcaa tatcttctag
     8101 tggatcaaca cgtccttcta cgtgagtaat attatcatca tcaaaacagc gaacaacgtg
     8161 acaaatagcg tctacttggc ggatatggct taagaattga tttcccaaac cttctccttt
     8221 acttgcgcct tttacgattc cagcaatatc tgtaaattca aaagtcgtcg gaacagtttt
     8281 tttcggatgt acaagttctg ttaagcgttg caaacgccag tcaggtactt ctaccatccc
     8341 tacgttgggg tcgatcgtcg caaagggata gtttgctgct tctgctcctg cttttgtgat
     8401 cgcattaaac aaagttgatt ttcctacgtt gggcaaccca acaattccag cagtcaatgc
     8461 cataaattta ttcactcttt ctctgcattt ttttcaatta ctttttttaa tttcttttca
     8521 aaatctcgac gggtcatcat gacgatatgt ccgcaattca tacatctgat tttgatatct
     8581 gcgcccattc gaatgacttc ccagcgattc gtctggcaag cgtgaggttt tttcatttca
     8641 acgatatcgt gcaagtcgta cataaaaata gcctcctttt tgtttattct tcatcaaatt
     8701 gaatattcaa gatatcaaga attcgagtca aatctttttg agaaagatat tcaatctcga
     8761 tcttaccttt cccatttttt tcttggatag ctacacttgt accaaattta tccatcaaac
     8821 gatcttcgct ttctcggata taatatggtt tttcctttgc tagccttggg atttttttct
     8881 tttcttttcc atcattttga tttaactcag aaacaagctg ttccagttgg cggacagtta
     8941 aattttcagt tactgcacga ttggctaatt tcaagatcaa tgttttattt tttaaaccta
     9001 acagcgtacg ggcttgtccc atagataaac gctgatcttg taccatttcc ttgacaagat
     9061 ccggtaaaga aagcaagcgc aaataattgg cgatataagg tctgctcttt cctaaacgac
     9121 tagctacttc tgcttgtgtt agtttcaggt ttttcatcag catctcatat gcttctgctt
     9181 cttctaacgg attcagatct tctcgctgca agttttccag cactgctact tgcatcatcg
     9241 cttcttcgtc aaagtctcta acgatagcag ggatcgtttc tttttcagct agtttggacg
     9301 ctcgaaaacg tcgctcccct gcaataatct catatccttt tactgcggaa cggcgtacga
     9361 tgatgggttg aaagacacct gattgttcaa tagaattagc tagttcctgc aaagatactt
     9421 cttcaaatgt tttacggggc tgatacggat ttggtcgcag ctcattcaat ggtagctgga
     9481 caacggtatc atcttttaca tccaccgtct ctaagttttc taaatcttga aacagtgcat
     9541 cgattcctcg gcctaatcct ttacttttct tcacgactta ccacttcctt tgccaatgct
     9601 tggtagactt cagctcctcg agaacgtgga tcgtagtcga tgatcggttt tccatgacta
     9661 ggtgcttctg ataaacgaat atttcttgga atgatcgttt cgtaaacttt ttcgcggaag
     9721 tatttacgta cttcttctac gacttcattt cccagatttg ttcgtgcatc atacattgtc
     9781 agtaagactc cctcgatctc taattctgga ttaaagtgtt tttggactaa acgaaccgta
     9841 ttcagtaatt ggcttaatcc ttccaatgcg taatactcac actgaacagg tatcaaaatc
     9901 gaatcacttg ctgtaaatga attgattgtc agatggccaa gagaaggcgg gcaatcgatc
     9961 aaaatataat catactgact gcttacttct gctaaagatc cttttaatct tgattctctt
    10021 gccatcattg acgtcaattc gatctctgct cctgctaatt ggagagtcgc aggcacaata
    10081 ctcaaatttt cgtgttcagt gatcaaagtt gcctcatcaa ttggcaattc attgactaga
    10141 acatcataga tgtctcttgt cacatcaggt tttctgatcc cgacaccact ggtagcattt
    10201 ccttgtgcat ccatatccac tagcaatact tttttgccaa ggctagcaag acaagcacct
    10261 agattcaccg tcgtcgttgt tttgccgacg ccaccttttt ggttcgcaac agatattatt
    10321 tgtgccatct cttctcctcc tatttgattg gctgcttatt aggcagtccg ggctttcttg
    10381 ggtatttctt tggggttggt ttcttttttt gaataacgat gatatgccgt tgatcagaga
    10441 cattcggcaa ggtaaccgcc tcttctttga ggaatttacc acctaataaa gcaatcgctt
    10501 gttttgcttc tgtcaattcc tcttcacttt ttgctgcttt caatgcataa aaatagcctt
    10561 cttgtttagc caagggcaaa cacaattccg ctaacacatt caaacgagca accgcacgag
    10621 cagtgacaag gtcaaatgat gcacgaaagt gaggattttg cccaaatgtt tctgcccggt
    10681 catgatataa ggaagcatcg attcctaata atcgggttaa ctcagataag aaggttattc
    10741 gtttattcag agaatcaacg atcgttatct gcagttctgg aaaggcaatt tttaaaggta
    10801 tgctaggaaa ccctgccccc gaaccaacat cgcataaaga taaaggttgc gtaaagtcta
    10861 cttcaaatgc taatgtaagt gaatcgtaaa aatgcttcag atatacgtct ttttcatccg
    10921 taatagctgt aaggttcatc ttttcgttcc attcgactaa tgtccgataa taaatagcga
    10981 attgattcat ttgatgatcg gtaagctcta tccctttttc tgccaaggat tggcgaaatt
    11041 cttctggtat cataaatatt cctttcattg tgaaacagca attgtttcac agattctctc
    11101 cctactttaa cgcaaaatct ctagacatgc aatccttcca tatggattct taaaaatttg
    11161 aaaagtaaga aaagccgata gactttttct ttctagtatg actcataaaa tatacacaga
    11221 agagagaact agctttgagc aatttctgca aaaactagtt ctctcttcta tttattatta
    11281 cgtattacgc tggttcactg actttcgcaa tcttcccttg ttcgatatag accattagga
    11341 tacttaggtc tgcagggttt acgccactga ttcggctagc ttgggcaatt gtttctggtt
    11401 ggattttctg caatttttgt tttgcttctg ttgctaatcc gttgattgct gcgtagtcga
    11461 tgttttctgg aatctttttc gcttccatgc gttttaattt ttctactttt tccatggctt
    11521 ttttgatgta gccttcatat ttgatttgga tttctacttg ttcgatttct tttcgtgtca
    11581 attcttcatt ttctggaata aaccgtagaa gttctgcata agtaatctca ggacgtctta
    11641 aaaagtcgct tgctagtaca ccatctttta atggtgccgc attaatcgtt gctaaaaagt
    11701 cttggacttc ttctgttggt ttgattcgta cattcatcaa acgtttgatt tcttgttcaa
    11761 ctgcttcttt tttcttcaaa taattcttgt attgatcttc ttttactaag cctacacggt
    11821 gacccatttc tgttaatcgc aaatctgcat tatcatgacg taaaagcaga cggtattcag
    11881 cacgtgaagt cagcaaacga taaggttcgt tcgttccttt tgttaccaaa tcatcgatca
    11941 tgacaccgat gtaaccgtca cttcgtttga tcactaaagg ttcttttccc tgaactttca
    12001 aagctgcatt gataccggca atcagccctt gtccagccgc ttcttcataa ccgcttgtac
    12061 catttgtttg tccagctgta taaagatttt caacgacttt agtttctaat gtcggacgga
    12121 gctggtgtgg ctctactacg tcatattcga tagcatatcc tgtacgcatc atttctactt
    12181 tttctaatcc agcaatagaa tgaagcatgt tgacttgaac atcctctggt aaagaagtag
    12241 ataaaccttg aacatagact tcttccgtat ttaagccttc tggttccaaa aataattgat
    12301 gacgtggttt gtctgcaaaa cgcacgatct tatcttcgat ggatggacaa tagcgagcgc
    12361 ctaccccttc aacgatccct gtaaacattg gggcacgatg taagttgtct cgaataattt
    12421 ggtgagtcgt ctcacctgta taggtcagcc agcaagattc ctgatctagt ttataggcac
    12481 tatctggtgt gctgaagcta aaatgattcg gctctttgtc accaggctgc tcttctgtaa
    12541 cagaatagtc gatcgtactt gattttactc gaggcggtgt tcctgttttg aaccgttcga
    12601 tagcaaagcc aagttctttt agatgattcg ctagtccaat agaaggttga gagttattcg
    12661 gacctgatga atatttcaat tcaccgataa tgatttctcc gcgaagtgct gttccggctg
    12721 taataacaac tgctttggcc cggtagctcg ctcctgtgga cgtcactact ccatgacaaa
    12781 caccatcttc gacgatcagt tcttcgacga ttccttgtct tagcgtcaaa tttggttctt
    12841 tttcgatcgt atgtttcatt tctgttgaat aagcatgttt gtctgcttgt gctcgcaatg
    12901 ctcggacagc tgggcctttt cctgtgttca acatacgcat ttggatatag gttttatcga
    12961 tattcttacc catttctccg cctaacgcat cgatttctcg aacgacaacg cctttagctg
    13021 gtccgcctac agaaggatta catggcatga atgctaccat gtccaaattt attgtcagta
    13081 ataatgtttt tgatcccata cgtgcagcag caagtgcagc ttctgaaccg gcgtgtcctg
    13141 cgccaacaac gatcacgtca tacgcatctg cttcataatg ttccaatcgt ttgttcctcc
    13201 ttttattttc ctaaacaaaa ttgactgaat aattgagtga ttagctcatc ttgtacactg
    13261 tcgccgacaa cttctcctag atagtcccag caacgcgtca tatctatttg aacgagatcc
    13321 acaggcattc ctgaatcaat acctgcaatg acttcttcaa gtgagaggga agctttttct
    13381 aacaacgcga tatggcgggt attagaaaca taagtagcgt ctctttctcc tgtttctccg
    13441 ctgaagaata aatcagaaat agctgattcc aaacgatcta gtccgtcgtt tttagctacg
    13501 gaaattgaaa aaactggttc attttcgatc aatttcttca atttttcttg ttccagctgt
    13561 gctggcaaat ctgttttatt tagaagaatg atccgtttca atccacttgt cgcttccaat
    13621 aattgttcat cttctgctgt cagtggttcg ctttggttta gaacaagtaa gatcaagtct
    13681 gcttctgcta atgcttttcg gcttctttcg accccgatcc gttccaccac atcttctgtt
    13741 tcccgaattc cagctgtatc gattagcttg agcggtaccc cccgaacatt gacatactct
    13801 tcgattacat ctcgagtcgt tcctgcaata tccgtaacga ttgctttttc ttctcttaac
    13861 aaatggttta acaaacttga tttgccgaca tttggccggc cgatgattgc cgtacttaac
    13921 ccttcccgca gaaccttccc ttgcttggat gtagcaagta gcgcttggat tctttgttgg
    13981 atcatctgcg ctttttcaag caataacttc gtagtcaatt cttcgacatc atcatattct
    14041 ggataatcaa tatttacttc tacttgtgct aatgtctcta aaatctcttg tctcaatgag
    14101 cggatcaaag cagacagatt gccgtctaac tgattaagag ctagtcccat tgctttgtct
    14161 gtttttgccc gaatcaaatc catcacagct tctgcctgtg aaaggtcaac acgaccattc
    14221 aagaatgctc gttttgtgaa ttcgcctggc tcagctaatc ttgcaccttc tcttaacaat
    14281 agctgcaaaa tttgattgac tacaacgatc ccaccgtgac aattgatttc tactacatct
    14341 tcccgcgtaa atgtctttgg tgctctcatc acggagacca tgacttcatc gacaagctga
    14401 ttgctcttgg gatcaacgat atgtccataa tggatcgtat gacttggtac ttctgataat
    14461 cgtttgtttc cagattgata aactttatct gctagttgca cagcttgatc gccacttaaa
    14521 cgaacgatac tgattgcacc ttctccaggt ggggtagaaa ttgccgcaat cgtatcaaat
    14581 tctaaagtaa tttcagccat tttttcactt cctttttcat tttgcgcaca aaaaaagtgc
    14641 ccactccacc ctttatgcac aataaacggt gaacttcgca ctttgactgc ttgttcttga
    14701 ttaaattctg gtcatagatt atcacacttg ctataaggaa tcaagtgttt aaaacaatta
    14761 gatgaagttg agcctgttct gcttccatta ccttatattt aattgtcctt ggatgtctct
    14821 ttgcgttctt tggtttattc gaagagaaga tactgtataa acaaggcttt atctttctta
    14881 aaaaaaacgg tacactaaat ggtagcgcta tctaaactat acacttttga aaggagaaaa
    14941 tatgtgagcc cgttcagatc tgctgcattg gaagaagaaa gtgaccctcg gatcaacgat
    15001 gtcgttttat ctacagggaa tatcaaccag aaatatatcg tccgcgatat cgtttttgtt
    15061 gtggaatgct tagaagctga tttgtttgac ccatcttttc ccaaagatga ccttttctta
    15121 acaacgaaga ggaaattaaa gacgaaggta cttgaatatg gtgcgaatgc agtaatcaac
    15181 tgtcattttg agtatcagca aaaaaaacgc gacggaaagt attttctgga actttttgct
    15241 tatggaacag tgatccaatt tatccaaaca acgattggtt aaatgaaaca gacaaaaaaa
    15301 gcggatcaaa actgaagaca gtttcgatcc gcttttttct atcaaagtta gtgactactt
    15361 catcgtatac tctgataaga atgatttcta gtaaaatata cttggtttga aaaactttgt
    15421 caacgtgatt tattcaaaaa gtaatggaat caataatatt ttttagcagg ttctactact
    15481 aaataacgat aaggttcctc accttccgaa tgtgttttta catgttcatc tttgcttaat
    15541 gcagcatgga tttgtttacg ttcaaatgca ggcattggtt ctaagaaaac aggctgccct
    15601 gtccgtttta ctttttcagc cgtgcgatca gctaaacgct gaatgatctc ttggcgcttt
    15661 tcacggtaat tgcctacatt gactactaca gacagcttat ttcttgctaa acgatggatg
    15721 aagacttgtg ccagatattg caatgcattc agtgtttttc catgtttccc aatcagtatg
    15781 ccttgctttt gtgtttctaa atggaaaaca accatattat ttgattctct ttccattttg
    15841 acaagagctg gagcatcaag ctgttttgaa atttcagtaa gataaactgc caattccgtt
    15901 aaagctgctt catcacctag ctcacttact tcttctgttt tttcttcaag aatcgtttcg
    15961 ttgactacct cgatctcagc tgttggttct tcttccaaaa tttcttctac agtctgttct
    16021 actgtttcag aaactttttc gctgatactt ggctggatcg aaagccgtgc attctttcga
    16081 cccattccta ggaaaccttt ttttccttct tccagtattt cgatttcgac ttggtctttg
    16141 gccaaaccca aatcagccag acctagttgg acagcttcgt ctattgttgc agcttcataa
    16201 atcggcatta gcattacctc ccctattatt tccgtttttt cttaggattc attgctttct
    16261 taagagcacg ttctctttcg cggattctcc gtgcttcttc ttcacgctct tttcgaattt
    16321 tgaaagggtt gttgatcagc agcgtttgta caacttggaa ggcattggaa actacccagt
    16381 ataatgaaag accgctggct aaattgacac ccatcactaa aatcattaca ggcatcacga
    16441 aattcatgat cttcattgat gcattcgatt caatttgact catgctagat aaatacgtac
    16501 ttgcaaaagt aaagattgct gctaaaatcg gcaaaataaa tgttggatcc gcgttcccta
    16561 agttcaacca taagaaatgc ccttgtttca actctggtac acgtgaaata gattgccata
    16621 acgccatcaa gattggcatt tgtactaata aaggaagaca accagcatat ggattgacgc
    16681 cattttctgc atataaacgc tgctgctctt ctcttaactt actttgtgtt tccggatctc
    16741 ttgaactata ttgttgctgc agtgctttta atttcggttg taattcttgc gttttacgca
    16801 tgctctttgt ttggaaatgc atcaacggca ataggatcac gcggatgatc agtgtaaata
    16861 aaatgatccc tatccctgca ttaccaaatg acaacgcctt gatcgcctca gcaaagtaat
    16921 aaacaatata acgatcccaa attcctgtac tctgtgcgct gacttcccca gttccgcaac
    16981 cggataagac gatgaccaaa ctaacgatac cggccatcaa aagtaaacgt ttgtatttct
    17041 tcacttactc ttttcctctc tgtcgattat ttttgcaagt tttaagacgt ggactaaatt
    17101 ggagcgaacc tcatcataag tcaacccctt cacacttggt ctagcaatca caataaaatc
    17161 caactccgga tcaatttctt cttttataga ataaactgct gctcggattt ttcgtttgac
    17221 ttcattgcgg tttaccgcgt tgccgacctt ttttcctaca gataaaccaa ctcgaaaatg
    17281 aggctgtccc ggtttttcca aacggtaaac gacgaatttg cggttagcaa aagaatctcc
    17341 cgtatggaac acggcttgaa actctcgttc tttttttact cgatacgctt ttctcattag
    17401 ttttctttcc ttttctatct ctcacttacg ctcataatca taccataatt acgattgtcc
    17461 tttcatcatt tccctaaaaa agagtataaa aaaaaccact gaaaccccag tggcctaagc
    17521 tgaaagaact tttcttcctt tgcgacgacg gctagctaac acacgacgtc catttttagt
    17581 actcatacgt ttacggaatc cgtgaacttt ttgacgttta cgtttatttg gttggtatgt
    17641 tcttttcata tattccacct ccaaaatgtt cttatctatt ttataaacac agacatactt
    17701 tgatagtata acgacaaagt agtgattttg tcaatataaa atagaagatc gattgttaaa
    17761 acttatccac aagattattt ttttctatgg gaatcctatg tggaaaaagt tatgcacagg
    17821 gattatccac agataataaa aggctggttt tacacagaaa acacacgtgt aaaaaaagta
    17881 atccacaatc aggcgatttc ctgtgtattc cgtctcctaa ctattggtat atctgtattt
    17941 tttatccaca gacgcaagag gaaatctatt ggtttttacg agttttccac ctgtgcatta
    18001 gtggtgtgga aaactttttt tgacaactgt gtttttctct tttttctttt tgtcgattgt
    18061 gtggataatt gtaattaaaa aatttttttc ttcggtttct gtggaaaact attgaaaaaa
    18121 atgctaaatt agtactagtt attcactttt taatgaagga ggaggcctta tatggtatcc
    18181 ctcgatgctt tatggaatga attaaaagca acataccaaa aggatttatc gcctgctagc
    18241 tataatacat ggatcgaaac agcgaaccct cgaacccttg atcaaaacca gctcgttgtt
    18301 gaggttccta gcaagattca taaagaatat tgggaaaaaa acctggcgac aaaaatcgtt
    18361 gaagtcggtt atatgttatc aggaaatgag attatccctc gctttattac tggcgaagaa
    18421 gcagagcaag aagaagttat agaagaaaaa aatccaaaag ttgtggcacc tagtccgcta
    18481 aaaaaagcca tgctgaaccc taaatatacc tttgatacgt ttgtcattgg taaaggaaac
    18541 cagatggctc atgcagcagc gctcgttgta gcagaagatc caggttctat ttataacccg
    18601 ctcttctttt atggaggcgt gggacttggt aagactcact tgatgcatgc aattggtcat
    18661 caaatgctgc aaagtcagcc taatgccaaa gtaaaatatg taagcagtga aacttttgct
    18721 aatgatttca tcaattcgat ccaaaacaaa acagcagaag agttccgtca agaatatcga
    18781 aatgttgatt tactgctagt ggatgacatt caattttttg cagaaaaaga agctacgcaa
    18841 gaagagtttt tccatacttt caatgccttg tacaatgaag ggaaacaaat cgtactaacc
    18901 agtgatcggt tgccaaacga gattccgaaa ctgcaagaac gccttgtttc tcgctttgca
    18961 tgggggctgt ctgttgatat tacaccgccg gatcttgaaa caagaacagc gattttacgt
    19021 aaaaaagcgg cggctgaacg tctagagatc ccagatgaca cactaagcta catcgcagga
    19081 caaattgatt ctaacattcg tgaattagaa ggtgcgcttg ttcgagtgca agcttttgct
    19141 gcgatgaata gtgaagatat ttcgactagt ctagctgcag acgcactgaa aacgttaaaa
    19201 tcaggaaaag gacatcctca attgtcaatc ctgcagatcc aagaagaagt ggcaaagtat
    19261 tatcacatcc aattgaaaga tctcaaagga aagaaacgcg tcaaatctat tgttgttcct
    19321 agacaaatcg ctatgtattt ggcacgtgag ctcacagata attctttacc gaaaatcgga
    19381 gctgaattcg gtggcaagga tcatacaacg gttatccatg ctcatgaaaa aatccagcag
    19441 ctgatggatt ctagtgtcag tatgcaaaat gaagtatcag aaatcaaaaa ctttttacta
    19501 aattaatttg tcgattctat caactgaaaa tagcatgtgg ataaaagaaa aaaacgttca
    19561 aaagttatac acactttttc cacagctgtt ttttcgtttc tctcttacgt tttttgagtt
    19621 ttccacagaa tcaacatgcc ctattacttt tattattctt tttaactaat aataaataaa
    19681 caaacattca aaggagatac atcatgaaag ttactttaaa ccgagctagc tttatgcagg
    19741 aattgcaaac tgttcaacga gctatttcaa gcaaaaccac gatccctatt ttgacaggtg
    19801 taaaaatcac actgacacaa gaaggtttga ctttgacggg gagcaacgct gatatatcaa
    19861 ttgaaacttt tttgtctgtt gaaaacgaaa aagcaaatat gcaaatcgaa tctactggtt
    19921 ccattgtttt acaagcacgt ttctttagcg aaatcattcg gagacttcct gaagaaacat
    19981 ttactttaga agttttagaa aataaacaag tagcgatcac ttctggaaaa gcgaatttta
    20041 tcgtaaatgg attagatgca gataactatc ctcatcttcc tgttgtcgaa agccataacc
    20101 agatgaaatt acctgtacac gtattgacta aactaatcaa cgaaacagtt tttgctgtct
    20161 cacaacatga gagtcgtcca atcttgacag gtgtgcattt cattttatct gataattctt
    20221 tattagctgt agctactgat tctcaccgtc taagtcaacg cgtgattcca gtagaacaag
    20281 cggctgatca ttttgatatt gttattcctg gaaaaagttt gatcgaatta tctcgctcat
    20341 taacaaatga agaagaaatc gtcgaaatca gcattatgga aaaccaagtg ttgttcaaaa
    20401 cagaaacgat gtatttctat tcccgtttgc tagaaggaaa ctatccagat accaatcgtt
    20461 tgattccttc aagttttaat acagaggttg aattttctgt tcctagcttt ttagcggcga
    20521 tcgaacgggc ttctttactt tctcatgaag gccgtaacaa catcgttcgt ttatctattc
    20581 gtccagatgc ggttgtttta tacggaaatt cgccagaaat cgggaaagta gaagaaagct
    20641 tgagttatac agcaagcagt ggcgatccat tagatatttc tttcaatccg gattatatga
    20701 aagcagcgtt gcgtgcattt ggagatatga gcatcaaagt gaaatttatc tctgctattc
    20761 gtccatttac attggaacca acggaagatg gcgttcaatt tattcagctg attactcctg
    20821 tacgtacaaa ttaacaatga ttttcaatgt tcttgaaaat ttaattgaaa agaaatagaa
    20881 aaaataaaat tttaaacaga aggccctaaa atctctttta aggctttctg tttatttttg
    20941 tttaaatcac cttttttaga ttaaatcgtc ttatatggaa aaatatatgg ataaatttgt
    21001 tttattgcga aaaaaaaggt ataatagagt atacttttaa taatgaaaat gagggatggg
    21061 cattgaaaca acaagtcatt ctaaatacgg aattcatgac gttgggccaa atgttgaaag
    21121 aagtcacagt tatcggtagt ggcggacaag caaaatggta tttggcagaa aacactgtgt
    21181 tagtcgatgg cgaaccagaa aatcgtcgtg gacgcaaact ttatcccggc atgatggttg
    21241 aagttccaga agtaggaact ttttttatgg taaaaaaagg aaacgacgat aatgaggctg
    21301 aataagctgt atttaaaaaa ttatcgaaat tacgaagagc tgaacatcga attttccaag
    21361 aacctcgtca tctttttagg agaaaatgcg caaggaaaaa cgaatctttt ggaaagtatc
    21421 tacgtattag ctatgacgcg aagtcatcgg acaagtagtg agcaggaatt gattggctgg
    21481 actgacgatc aggcgatgat tcaaggagag atcacaaaag gttcatccac tcttccatta
    21541 gaaatcttat tatctaaaaa aggccgaaaa accaagatca accatatcga acagaaaaag
    21601 ttaagtactt acgtgggaca aatgaatgtc attttatttg caccagaaga tctttcttta
    21661 gtaaaaggca gcccgcagac caggcggaag tttttagata tggaattagg ccagatcaat
    21721 ccaatctatc tttatgatct tgtccaatac caagcaattt tgaaacaaag aaaccagtac
    21781 ttgaagcaat tgaatgaaaa aaaacaaact gatttgcttt atttggatat tttgtccgaa
    21841 caactagcag cgtttggcag caaagtattg aaagcaagag cacagttcgt caagcgattg
    21901 gagttttggg ctaattcttt gcatcagcaa atcacccatc aaaaagagca gctggaaatc
    21961 gagtatctca ccgcagtcga ttctttagaa acacatacgc aagagcaaat ccaagaacaa
    22021 tttctcgcac ttttgaacca aaataaaaag aaagaccttt ttcggggaac aacgacagtt
    22081 ggtcctcatc gtgacgatct ttcctttttt attaatcaga aaaatgtgca gacttatgga
    22141 tcgcaaggac agcaaagaac aactgcacta agtgtcaaat tagcagaaat cgatttgatt
    22201 aaagaagaga caggggaata tccaatcctt ttgttagatg atgtgatgag cgagctggat
    22261 gataatcgtc aactccatct gctagaaaca atcgaaggaa aagttcaaac gttcttaaca
    22321 acaacgacac ttgaccatgt aaaaaataaa atgactgttg aacctgagat tttttatgtt
    22381 caacaaggga aactaaaagg agaagaaact gaatgacaga agaaagaagt ttagtagaac
    22441 gcgctaaaga gtatgatgcc agtcagattc aagtacttga aggacttgaa gccgttcgta
    22501 aacgacctgg catgtatatc ggttcgacta gttctgaggg tctgcatcac cttgtttggg
    22561 aaattgtcga taattcgatc gatgaagtat tagcaggttt tgcaacgaaa atccatgtga
    22621 ttattgaaaa agacaacagt atcaccgtta ttgacgacgg tcgggggata cctgtagata
    22681 tccaggcaaa aactggacgt cctgcagttg aaacggtctt tactgtttta catgctggag
    22741 gaaaattcgg cggtggtggg tacaaagttt ctggtggact tcacggagta ggatcttctg
    22801 ttgttaacgc gctttctaca cagcttgatg tcaaagtata caaagaagga aatgtgtact
    22861 atcaagaata tcgtcgtgga gctgtagtgg acgacttaaa gattatcgaa caaacggatc
    22921 gccatggaac aacggttcac tttacgccag accctgaaat ttttacagag acaactgagt
    22981 ttgatttttc aaaattggct acccggatcc gtgaacttgc tttcttgaac cgcgggatgc
    23041 gtatatcgat tgaagacaaa cgagaagaag aaccagtcat caatgaatac cattatgacg
    23101 gcgggattaa aagttatgtc gaatacttga atgcaaacaa aaccgtgatt tttccagaac
    23161 ctgtatattt ggaaggtgaa cagcaagata tcgcagttga agtctctatg caatatacag
    23221 acggctacca ttccaatatc atgagttttg ccaacaatat ccatacgtat gaaggcggaa
    23281 cacatgaatc aggattcaag actgctttaa ctcgtgtgat aaatgattat gcccgaaaac
    23341 aaaagctgat gaaagaaaat gacgacaact tgaccggtga agacgtgcga gaaggattga
    23401 cagctgttat ttctatcaag catcctgacc ctcaattcga aggacaaaca aaaacaaaat
    23461 tgggcaattc cgaagtacgg acagtcactg atcggttatt tagcgaacat ttcatgaaat
    23521 tcttgctgga aaacccaagt gtgggccgtc aaattgttga aaaaggatta ctagcttcca
    23581 gagctcgtct agcagcgaaa cgtgcgcgtg aagtaacgag acgtaaaggt gctttagaga
    23641 ttagtaatct gccaggaaaa ttagcggact gctctagcaa cgatcctgaa aaatgcgaat
    23701 tgttcatcgt cgaaggagat tcggccggcg gatcagctaa gcaaggtcgt aatcgagagt
    23761 ttcaagccat cttgcctatt cgtgggaaaa tcttgaatgt tgaaaaagca agcatggaca
    23821 agatcttggc aaatgaagag atccggtctt tatttactgc tatgggtaca gggtttggag
    23881 aagattttga tgtatcgaaa gctcgctacc acaaattagt tatcatgact gatgccgatg
    23941 tcgacggcgc gcatatccgt acattgctgc tgactctttt ttatcgttac atgcgtccta
    24001 ttgttgaagc tggatacgtc tatattgctc agccaccttt atacggggta aaacaaggga
    24061 agaatattac gtatgtacaa ccaggaaaaa atgcagaaga agagttgaaa caagtcgttg
    24121 cttcacttcc tgcctcacca aaaccaagcg ttcagcgtta taaaggactt ggagaaatgg
    24181 atgatcatca actatgggaa acaactatgg acccaagcaa tcggatgatg gcccgcgtca
    24241 gtgtggacga tgctattgca gcagatcaga tatttgaaat gttgatgggg gatcgagtag
    24301 aaccccgtcg tgcattcatc gaggaaaatg cacactatgt gaagaactta gatatttaat
    24361 catgaaggag gaaaaagaat gagtgaagaa atcagagaaa acatccatga tgtcaatcta
    24421 accagcgaaa tgaaagactc cttcatcgat tatgcgatga gtgttattgt cgcacgtgca
    24481 cttcctgatg tcagagatgg tctgaaaccc gttcatcgcc gtattttata cggcatgaac
    24541 gaattaggtg taacaccgga taaagcccat aaaaaatctg ccagaatcgt cggagatgtc
    24601 atgggtaaat accatcctca tggagactat gcgatctatg agtcaatggt acggatggca
    24661 cagccattca gctaccgtta tatgctagtg gatggtcatg gaaactttgg ttctgttgac
    24721 ggtgacggtg ccgccgctat gcgttatacc gaagcacgga tgagcaaaat cgctacagaa
    24781 atgctgcgtg atatcaataa gaacactgtt gatttccaaa gcaactatga tgatacagaa
    24841 aaagaaccag ttgtactacc tgctcgcttc ccgaatcttt tagtgaacgg aacaacgggg
    24901 atcgccgtag gtatggccac gaatatccca cctcataact tgtctgaggt agtagcggct
    24961 atcgatttgc tgatggataa tcctgatgtg acgaccaatg aattgatgga agtattacct
    25021 ggaccagatt ttccaacagg tggtttggtt atgggaaaat caggaatccg aagagcgtat
    25081 gaaacaggta agggttctat taccgtacgt gcaaaagttg agattactga gatgccaaat
    25141 ggtaaagaac ggatcatcgt gacagaattg ccttatatgg tcaataaagc aaaattgatc
    25201 gaacggattt ctgaactgca cagagacaag cgtatcgaag ggatcacaga tttgcgggac
    25261 gaatcttccc gtgaaggtat gcggatcgtc attgacatcc gtcgtgacgc cagcgcatct
    25321 gttatcttga ataacttata taaattgaca tccttgcaaa attcatttgg attcaatatg
    25381 ttggcgatcg aaaaaggtgt gccgaaagtt ttgagcttga aacaaattct tgaaaactat
    25441 atcgaacatc agcgcgaagt catcacaaga cggacagtct ttgaaaaaga aaaagcagaa
    25501 gcaagggcac acattttaga aggtttgcgt attgctttgg atcatatcga tgaaatcatt
    25561 gcgattatcc gcgggtctaa atcagatgac gaagcaaaag cgacgatgat cgaacggttc
    25621 gatttatccg atcgccagtc tcaagctatt ttagatatgc gtttacgccg attgaccggt
    25681 ttggaacggg aaaaaatcga aaatgaatac caagaactct taaaactgat tgaagattta
    25741 actgacattc tggcaagacc agaacgtgtt acagaaatca tcaagaatga attagcagac
    25801 ttgaatcaac gatttggtga taaacgacgc acagaattac tagtcggtga ggtcttaagt
    25861 ctggaagatg aagatttaat cgaagaagaa gaaatcgtta ttacattgac aaacaatggt
    25921 tacatcaaac gtttggcaaa taatgaattc agagctcaac gaaggggtgg acgtggtgtc
    25981 caaggaatgg gtatccacaa tgacgacttc gtcaaaaatc tggtttcttg ttctacccat
    26041 gatacattgt tattctttac gaataacgga aaagtgtatc gggcaaaagg atacgaaatt
    26101 ccagaatacg ggcgcacagc caaaggaata ccaattatca atttactagg tatcgattcg
    26161 agtgaaagca tccaggcaat cattgcggtg acgggagaag cagaagaagg ccactatctc
    26221 ttctttacga cgcgtcaagg tacagtaaaa cgtacttctg tcactgcttt tgctaacatc
    26281 cgaagtaatg gtctgatcgc aattggtttg aaagaagacg atgagctcgt caatgtgcta
    26341 ctgacagatg gtcaatctaa tatcattatt ggaacacata acggctactc tgtcactttc
    26401 aaagaagaag cagtccgcga tatgggacgt acagcttccg gggttagagg aatacgcctg
    26461 cgtgaggaag attatgtcgt tggggcagcc ttaatgaagg aagatcaaga agtattagtg
    26521 atcacagaaa aaggttacgg aaaacggaca aaagtatcgg aatatccagt gaaaggccgc
    26581 ggaggtaaag ggatcaaaac ggcgaacatc actgaaaaaa atggtccact agctggattg
    26641 accactgttt ctggagatga agacatcatg ctgattacag ataaaggtgt cattatccgt
    26701 ttcaacgtat cgactgtttc tcaaactgga cgttcaacat taggcgtacg tttgatgaag
    26761 atggaagctg atacgaaagt tgtcacgatg gcagcagtcg aaccagaagc tgcagaacaa
    26821 acagttgaag aacaaggatc agaagaatag ccatcaagac taattgtgta agctagcaag
    26881 aatgaccgat taaaaaatct ggttattcct gctggctttt ttgattagtt gaatttctct
    26941 taaaatattc gaaactgttc attctgtatt gcttttgacc aaaatagagt gtataatcag
    27001 agcttgtgag tagtctaatt aattagataa ctctccttgc tcttaggaac ctaagagccg
    27061 aaagtccaca gggaggtgaa tatcaatgga aaacacgaaa tatgaaatca tgtacattat
    27121 tcgtccaaac attgatgaag aagcaaaaac tgctttaatc gaacgtttcg atgcaatctt
    27181 gaaagataac ggagcagaag ttatcgaatc aaaagattgg gaaaaacgcc gtttcgctta
    27241 tgaaatgaac ggttaccgtg aaggtatcta tcacatcgtt aaagtatctt ctccatcatc
    27301 agcaaatgct gtcaatgaat ttgaccgtct tgctaaaatc aacgacgata tcattcgtca
    27361 catgattgtt aaagaagaag cttaattgtt gtttcatgtg aaacaataga aaacgatgag
    27421 aggagatgac tattttgatt aataatgttg tattagttgg acgtctgacg aaagatccag
    27481 atttgcgtta cacagcaagt ggtacggcag tcgcaacatt tactttggct gtgaatcgta
    27541 attttacaaa ccaaagcggc aatcgcgagg ctgattttat caactgtgtg atttggcgca
    27601 aatcagcaga aactttagca aactatgctc gtaaaggaac acttttaggt gttactggac
    27661 gtatccaaac tcgttcttat gataatcagc aaggacaacg tgtatacgtg acagaagtag
    27721 tagctgacaa cttccaattg ctggaaagtc gttctgcatc tgaaaatcgc caacaaagcg
    27781 gcggattcca aagttctggt caatcagctg gcggatttgg cggaaacaat aactctaatc
    27841 aaacatctca atcatccaac ggtatgcctg atttcgaccg tgatacttct gacccattcg
    27901 gctcaagttc aacgatcgac atttcagacg atgatttacc attctaaacg aatttgatag
    27961 gagggaaata gaatggcaca acaaagaaga ggcggacgta aacgtcgtaa agtagactac
    28021 attgctgcta accatattga atatatcgat tataaagata tcgaattact aaaaagattt
    28081 atttcagaac gcggtaaaat cttgccacgt cgtgtaactg gtacaggcgc taaaaaccaa
    28141 cgtaaaatta ctgttgcaat caaacgcgct cgtatcatgg gcttgctacc tttcgttagc
    28201 gaagactaat aaatcctagg aattgaatga ctgatccttt tgatcagcta ttcaattcct
    28261 ttttttctgt aaaaataacc tctattcgtt tagtcgctcg aatgacgaaa gttatggtaa
    28321 aatcaaatta gtcatatatg ggaggaaaat tatggaaaat aaaaacaatc atttatcccc
    28381 gaaacctctc ttccttatcc tgtttatatt aatcgaagta ttggtagtct taattttacc
    28441 atttcgtttt gttagcgctt cggtcattct attgatgaat ttatggggtg ttcgctattg
    28501 gttgaaaatg aaacgttggc ttattttaaa taaacgtgaa aaaatccagc aggcttcgac
    28561 tgtagctgaa accaatctag cttatgtctc agaagtcatg cctgtaggag tgattgctta
    28621 tcagccatca agccaaaaaa tcgaatggct caatccatta gcattgaaaa tcaaagatca
    28681 aagtggatta acagaggaag aattgctaga tacttttttt actgcaaaag aaaaaaagaa
    28741 aaaccaagtc tatctagcag ataaaacatt tcagtttgat atggatacag taaagggtgt
    28801 tgtttatttt aatgatatta cccaaaacca gttgttaaaa ctccatcaga cgaacagtca
    28861 gccggtgatt ggtattttat ctattgataa ctatgcagat gccattgata aattggatga
    28921 caaagagatt tcttatttga actctttcgt cactacacta gtttctgact ggatgaatga
    28981 ataccatgtc tttttcaaaa gaatcaacgc agaacggttt ttcttcgtag cacagatgat
    29041 cgatctgaaa aagatgatgg ataaaaaatt tgatttgctg gatcgctttc gagcagaagc
    29101 tgaaaaacag agctcgccta tcacccttag tatggggatt gcttatggct caagaaccct
    29161 cgatgaaatc ggtggagaag ctcagaataa tttagatgtt gctttagtcc gtggaggcga
    29221 ccaagttgtc gtgaaagagg taaaagaagg agcaaaacct gtttattatg gggggaaatc
    29281 tgctaagaat gtcaaacgca cgcgtgttcg ttctcgagcg atgagtaccg ctttgcgtcg
    29341 gatttttgat gaaacaggcg atgtgtttat catggggcac aagtttccgg atatggatgc
    29401 tttaggctct gcttttggtg tcagttgtct ggcacgcttc aaccagaaaa aagcttacat
    29461 cgttatcaac gagaacgaga tcattccaga tgtagaacgg tgtcttgaag aaatccataa
    29521 gcatcctgaa ttagaaatgc aactaatctc tccagaacgt gccatagagc tgaaaaaaga
    29581 agaggacctc ctcgtgatgg tggattacca taaaccttct ctatcgatct ctcagggcct
    29641 atatgagcaa tttgataaga ttgtgatcat tgatcaccat cgtcgtgggg aagagtttcc
    29701 aagtaaacct ctgctgactt atatcgaatc ttcagcttct tcagcttcag agctagtttc
    29761 tgagctgatc caataccaaa gcagcaaaac aaatcgctta gatcgttttg aggccacatt
    29821 attgcttgca gggatggttg tagatacaaa tagtttcaat gtccgtacaa ctgcacgaac
    29881 ttttgacgtt gccagctatt taaggtcttg tggagcagac gcatccttag tacaatattt
    29941 attgagctcg gatttagcga catacttgga gatcagcaat ttgattgctg gaagcgacta
    30001 tgtgacttct gatattgttg tggcggcagg gacagacgaa aaagagtata atagtgttat
    30061 agcagcaaaa acagcagata cattgctttc gatgatcaat atcaatgcag ctttcgtcat
    30121 tacaaagcgg acagacggct tgattgggat cagtgccaga agtaccggca cgatcaacgt
    30181 ccaaatcatc atggaatcct taggtggcgg aggacacttc acaaacgcag ctactcaact
    30241 ggaaaatacg acagttgagg aaacaaaaca gaagctattt gctgcaatca aagaaaatat
    30301 gaatcaaata tatgataagg agtaatccaa atgaaagtaa tttttttaca agatgtaaaa
    30361 ggaaaaggta aaaaagggga catcaaagaa gttccagcag gttatgcaca aaattatcta
    30421 atcaaaaata acttggcaaa agaagcaacg aaaagcagta tcagtcagtt agcaggacaa
    30481 aagaaagcac aagaaaaaca cgaagctgaa atcttggaag aagctaaaaa gttaaaagaa
    30541 ttcttggaaa aagaagatac tgtagtcgaa ttgaaggcga aagcagggga agacagccgt
    30601 ttgtttggtt cgatcccttc aaaacaaatt gctgaagggt taaacaaaca atacaaaatc
    30661 aaattagata aacgaaaaat cgaattagaa aatccgatcc gttcactagg ctacacaaac
    30721 gtacctgtta agcttcatca tgaggtgacg gctacgatca aagttcatgt cgtacaagaa
    30781 taacaaatct tgatagcgat gttcagctgg taagtataac cagaaggaaa aaagacaaac
    30841 cgaaatttat ctgagaaaac agctgcagca atgaaagcaa aagctgcaaa tcgatttcag
    30901 attgatttcg gtttcttttt tgggaacagc aaaatagcat aaagaaaacg agtataataa
    30961 aagagaacta tttttttatt acactttcgg tacgaatgat gacttttctc tcattttctt
    31021 gtaaaatgaa gcaattgaag acaaggagcg atacgtatga atgaagtgtg gcaggatcga
    31081 atcccaccgc aaaatattga agctgaacaa gcggttttag gctctgtttt tcttgacgca
    31141 gaaacgatca ttgatgccat ggagtacata gagccaaaag acttttatcg tcgtggccat
    31201 caaattattt ttgagacaat gatcgagtta aatgatcgta atgaagcgat tgacgtggtt
    31261 actgtcaaag accggctgga acaagccaat ttgttagaag atgccggtgg tttaagttat
    31321 ctctcagact tggcattagc agttccgaca gcagcaaata tcgtctacta tgcgaaaatc
    31381 gttgaagaaa aatcattgct gcgtacattg atccagacag caacaggaat cgttacgaaa
    31441 ggattcgagc aaggagaaga agttcaaaca attttagatg atgccgaacg cagtatcttg
    31501 gaggtttcgg aaaaaagaaa tcgaagcggc tttttatcta ttgctgaagt actgaactcc
    31561 acgattgaaa atatcgatcg actagctcaa aacaatgaag aaatcacagg tctaccaact
    31621 ggctatcaag gtctggataa aatgacagca ggtttccagc ccgaagaact aatcattctt
    31681 gctgcacgtc cagcggtagg taaaacagcg tttgcattga atattgctca aaatgtagga
    31741 acaaaaacag atcgttctgt tgcgatattt agtttggaaa tgggggcaga atctcttgta
    31801 aatcggatgc tttgtgcaga aggttcgatt gaggccagcc atttacgtac aggtcagctt
    31861 tcggaagaag aatggtcgaa tctgatcgtt gcgatgggga gtctttctcg agcaaatatt
    31921 tttatcgatg atacaccagg gatcaaaatc tctgaaatcc gtgcgaaatg tcggaaacta
    31981 gcgcaagaaa aaggcaatct aggcttgata ttgatcgatt atttgcagtt gattgaaggt
    32041 aacggtaaag agaaccgtca acaagaagta tcagatatct ctcgccaact gaaaaaactg
    32101 gcgaaagagc tgaaagtacc tgtcattgct ctttcacagt tatctcgtgg agtagaacag
    32161 cgacaagaca aacgaccagt gctaagtgat attcgtgaat ccggatcaat tgaacaagat
    32221 gcggacatcg tagccttctt gtatcgagat gattattatg aaagagaagg cggagatgac
    32281 gatgatggaa acgagccaaa aaatgacaac gtcatcgaag tcatcatcga gaagaaccga
    32341 agcggtgcac gaggaacagt tgaactactg tttatcaaag aatacaataa gttttcgtcg
    32401 ctttcgccaa gagaagaatt ttaaagaaga ggttgtgagc cttgagcaat cccgagagct
    32461 ctcgtaagaa gatgcaagga gtaaccgcag gaacgactag agtacttaat ttaaacagat
    32521 ctccaaaggg attgtgacaa aagtcttgtc acagtccctt tttccgaata aacggtgttc
    32581 aatcagctga cccttggtat tagctcagca aacgtcaaaa tatgaggaac tattttgacg
    32641 tttgtttctc taatactcag gtcaaaacgc tgatcttacg acctcttttc cttaaacggt
    32701 gttctcctgc caactcctcg gtcacaagtc acaatatcat caaacatgag aagcagtttt
    32761 ttgaaattgc tccttgtgac tcagagttaa acggcaggct cacaacctct ttcttaaacg
    32821 gtgttcaaaa gcttgcatct gcggtaatac gcccaatcaa acgaaaaata tgagaagcta
    32881 tttttgtttg gctgttctta ttacttgatg caggacagct ttttcacaac ctctgattca
    32941 cggtgttcca aaagtagctt cttcgaaaaa taagtcgaaa tttcacaaaa atttgagaag
    33001 caattttcgt acattccttc ttatttcttg aagctgacca cttctgttac aacctctttt
    33061 ttaataacca ttctaaaaag ataaagttcg ttttttaagc gtttttataa aataaaaata
    33121 aaaatcattc gattttattt gaaatatgta tctattattg atacaatggt tcaggattaa
    33181 ataatataga acgaggtgtt cacatgtcat ctgtagtagt agtaggtacg caatggggcg
    33241 atgaagggaa aggaaaaatc acagactttc tcagtgaaaa tgctgaagtg attgcacggt
    33301 accaaggtgg cgacaatgca gggcacacaa ttaagtttaa cggtgtgact tataaactgc
    33361 atttgattcc ttctgggatt ttttataaag acaagatcag tgtgatcgga aacggcgttg
    33421 tagtcaatcc taaatcattg gttaaagaat tagcttattt acatgaaaaa ggtgtagaaa
    33481 caagtaattt gcgtatttct gaccgcgcgc acgtaatctt gccttatcat atcaaattgg
    33541 accaattaca agaagatgct aagggcgaaa ataagattgg tacaacgatt aaaggaatcg
    33601 gacctgccta tatggataaa gcagctcgtg taggtatccg tattgcagac ttattagata
    33661 aagaaatttt tgaagaacgt cttcgtttga atctagatga caaaaaccgt caatttgtaa
    33721 aaatgtttga ttcagaacca cttgattttg acgatatttt tgaagaatat tatgaatacg
    33781 gacaacaaat caaacaatac gtcacagata cttccgttat cttgaatgat gctttggatg
    33841 caggcaaacg cgtgttgttt gaaggcgcac aaggtgtaat gctggatatc gaccagggaa
    33901 cttatccttt tgttacttct tctaacccag tagccggtgg ggtaacaatc ggaagcggtg
    33961 tagggccttc aaaaatcaat aaagttgtcg gcgtctgcaa agcctatacg tcacgtgtcg
    34021 gtgatggacc atttccaact gaattatttg atgaaacagg agaacaaatc cgcgaagtag
    34081 gacgagaata cggtacgaca actggtcgtc cgcgtcgtgt aggctggttt gattctgtag
    34141 ttatgcgcca ttctaaacga gtttcaggta ttaccaacct atctttaaac tcgatcgatg
    34201 tattaagtgg attggaaaca gtaaaaattt gtacggctta tgaattagat ggcgaactaa
    34261 tctaccacta tccagcaagc ttgaaagagt tgaaccgctg caaaccaatc tatgaagaat
    34321 tgccaggctg gtcagaagac attacaggat gcaaaacact tgcagaactg ccagaaaacg
    34381 cacgtaacta tgtacgtcgt atctcagagc ttgttggtgt acgtatctca acattttctg
    34441 tcggacctga tagaacacaa acaaacatct tagaaagcgt ctgggcgcaa atctaaggat
    34501 aaaaacgacg actgaatcag gacgttctta ccagagcgag tcgaagtgtt tatctatacg
    34561 ataataataa cttgaacact gtttattcag aataagggac tgtgacaaaa accgtgtcac
    34621 agtccctttt tccgaa
//
LOCUS       DACDXO010000024        33820 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E23605_2017
            SAMN12639989-rid9881463.denovo.024, whole genome shotgun sequence.
ACCESSION   DACDXO010000024 DACDXO010000000
VERSION     DACDXO010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639989
            Sequence Read Archive: SRR10023389
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 33820)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33820)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741914.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:42:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,972
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 33 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 17 of 73
            Pseudo Genes (multiple problems)  :: 10 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33820
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639989-rid9881463.denovo.024"
                     /isolate="E23605_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(78..635)
                     /gene="frr"
                     /locus_tag="IWM35_001342"
     CDS             complement(78..635)
                     /gene="frr"
                     /locus_tag="IWM35_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HAP9919202.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
     gene            complement(638..1360)
                     /locus_tag="IWM35_001343"
     CDS             complement(638..1360)
                     /locus_tag="IWM35_001343"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010761216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HAP9919203.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            complement(1496..2377)
                     /locus_tag="IWM35_001344"
     CDS             complement(1496..2377)
                     /locus_tag="IWM35_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HAP9919204.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            complement(2476..3258)
                     /gene="rpsB"
                     /locus_tag="IWM35_001345"
     CDS             complement(2476..3258)
                     /gene="rpsB"
                     /locus_tag="IWM35_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HAP9919205.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            3617..4096
                     /locus_tag="IWM35_001346"
     CDS             3617..4096
                     /locus_tag="IWM35_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgR family transcriptional regulator"
                     /protein_id="HAP9919206.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            4313..5404
                     /gene="phnW"
                     /locus_tag="IWM35_001347"
     CDS             4313..5404
                     /gene="phnW"
                     /locus_tag="IWM35_001347"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HAP9919207.1"
                     /translation="MYKLLTSGPLTTSETVKNAMLIDHCTWDQEYKEITQWIRKKLLD
                     LAQVSEGIYTTVLMQGSSSFGVESVLSSVIRPEDTVLICSNGAYRQRMINMCECLKLN
                     YTVYEVSEHQILQAHVITQLIEENPSISALAMVHSETTSGILNNLEEIAAVTQERDLL
                     FIVDAMSSFGGIPICVAELGIDFLISSSNKCIQGVPGFSFVLANKAELEKRKNVSRSL
                     SLDLYDQYRVMENENGKWRFTSPTHTVLAFQQALKELEAEGGIETRYSRYLANNHVLR
                     QKMTEMGFQPFIQEYQGPFITSFLYPKDWRFDFNHFYQYLKQNGFVIYPGKISDHECF
                     RIGNIGEIYPEDILELTAIIKDYKENVND"
     gene            5397..5525
                     /locus_tag="IWM35_001348"
     CDS             5397..5525
                     /locus_tag="IWM35_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HAP9919208.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGIQLTDKEIR"
     gene            5529..6074
                     /locus_tag="IWM35_001349"
     CDS             5529..6074
                     /locus_tag="IWM35_001349"
                     /EC_number="3.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HAP9919209.1"
                     /translation="MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEH
                     YLFKDLVKHSTLKPDTLETIERLRNAGINIGSTNGFNAPMMTIVAETAKKERYSPDFM
                     ATFEDVEGYGRPFPYMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEG
                     SSLMGISYDEYQKLEMGKKIV"
     gene            complement(6528..8219)
                     /locus_tag="IWM35_001350"
     CDS             complement(6528..8219)
                     /locus_tag="IWM35_001350"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HAP9919210.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDKEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMHHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(8639..9586)
                     /gene="arcC"
                     /locus_tag="IWM35_001351"
     CDS             complement(8639..9586)
                     /gene="arcC"
                     /locus_tag="IWM35_001351"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HAP9919211.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(9701..10720)
                     /gene="argF"
                     /locus_tag="IWM35_001352"
     CDS             complement(9701..10720)
                     /gene="argF"
                     /locus_tag="IWM35_001352"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HAP9919212.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(10811..12040)
                     /gene="arcA"
                     /locus_tag="IWM35_001353"
     CDS             complement(10811..12040)
                     /gene="arcA"
                     /locus_tag="IWM35_001353"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HAP9919213.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
     gene            12501..13202
                     /locus_tag="IWM35_001354"
     CDS             12501..13202
                     /locus_tag="IWM35_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAP9919214.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
     gene            complement(13811..14827)
                     /gene="tsaD"
                     /locus_tag="IWM35_001355"
     CDS             complement(13811..14827)
                     /gene="tsaD"
                     /locus_tag="IWM35_001355"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HAP9919215.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIIAGGVAANQ
                     GLREGLQDALAAKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
     gene            complement(14824..15288)
                     /gene="rimI"
                     /locus_tag="IWM35_001356"
     CDS             complement(14824..15288)
                     /gene="rimI"
                     /locus_tag="IWM35_001356"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HAP9919216.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLAEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            complement(15295..15837)
                     /gene="rimI"
                     /locus_tag="IWM35_001357"
     CDS             complement(15295..15837)
                     /gene="rimI"
                     /locus_tag="IWM35_001357"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HAP9919217.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTVEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            complement(15821..16645)
                     /gene="tsaB"
                     /locus_tag="IWM35_001358"
     CDS             complement(15821..16645)
                     /gene="tsaB"
                     /locus_tag="IWM35_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HAP9919218.1"
                     /translation="MYYCTSFAFGIFILRIKKYATMEGENVIAERIKYMITLGIDTAN
                     QTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAIDQLFADLQISPKDIDRIAVSDGPGS
                     YTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAANCIGVDNIIVPMFDARRKNVYAGAY
                     RWKENRLETVLEDVHISASDLFTQLQKLNEAIYFVGSDCHKFFSEIKEILPSAMINTV
                     PAWDIPSGVTVAQLGALVEPVAEKQAFLPRYLKRVEAEEKWLETHTPGDESYVEKI"
     gene            complement(16734..17714)
                     /locus_tag="IWM35_001359"
     CDS             complement(16734..17714)
                     /locus_tag="IWM35_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAP9919219.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPADGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
     gene            complement(17738..19222)
                     /gene="galT"
                     /locus_tag="IWM35_001360"
     CDS             complement(17738..19222)
                     /gene="galT"
                     /locus_tag="IWM35_001360"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HAP9919220.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLEDYPQVIAGVVKWPMSVIRLQSTEKNLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            complement(19234..20223)
                     /gene="galE"
                     /locus_tag="IWM35_001361"
     CDS             complement(19234..20223)
                     /gene="galE"
                     /locus_tag="IWM35_001361"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HAP9919221.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREILGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
     gene            20471..20638
                     /locus_tag="IWM35_001362"
     CDS             20471..20638
                     /locus_tag="IWM35_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HAP9919222.1"
                     /translation="MKKFVSGILVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEEN
                     RKKAMRKRVSR"
     gene            complement(20700..22511)
                     /locus_tag="IWM35_001363"
     CDS             complement(20700..22511)
                     /locus_tag="IWM35_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HAP9919223.1"
                     /translation="MKIGIIGAGPRGLSVAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNVLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSRGGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGAMAWPRLLTKENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLETPLAYQNEPFSEASRIFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPIIRTKELAALIDAGIFHLIEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(22508..22873)
                     /locus_tag="IWM35_001364"
     CDS             complement(22508..22873)
                     /locus_tag="IWM35_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HAP9919224.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            complement(23036..23431)
                     /locus_tag="IWM35_001365"
     CDS             complement(23036..23431)
                     /locus_tag="IWM35_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAP9919225.1"
                     /translation="MVLWVINIILLLIVLAIILWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            complement(23449..24411)
                     /locus_tag="IWM35_001366"
     CDS             complement(23449..24411)
                     /locus_tag="IWM35_001366"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HAP9919226.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRFLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACGNLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            complement(24411..24623)
                     /locus_tag="IWM35_001367"
     CDS             complement(24411..24623)
                     /locus_tag="IWM35_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HAP9919227.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            complement(24644..25342)
                     /locus_tag="IWM35_001368"
     CDS             complement(24644..25342)
                     /locus_tag="IWM35_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HAP9919228.1"
                     /translation="MNYQQQIKLRAWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            complement(25362..25904)
                     /locus_tag="IWM35_001369"
     CDS             complement(25362..25904)
                     /locus_tag="IWM35_001369"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HAP9919229.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            complement(26036..27040)
                     /locus_tag="IWM35_001370"
     CDS             complement(26036..27040)
                     /locus_tag="IWM35_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HAP9919230.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIVLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILPSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            complement(27037..28026)
                     /locus_tag="IWM35_001371"
     CDS             complement(27037..28026)
                     /locus_tag="IWM35_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HAP9919231.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVEKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            complement(28023..28829)
                     /locus_tag="IWM35_001372"
     CDS             complement(28023..28829)
                     /locus_tag="IWM35_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9919232.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKIALLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            28995..29951
                     /locus_tag="IWM35_001373"
     CDS             28995..29951
                     /locus_tag="IWM35_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP9919233.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            complement(30028..30546)
                     /locus_tag="IWM35_001374"
     CDS             complement(30028..30546)
                     /locus_tag="IWM35_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAP9919234.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(30634..30783)
                     /gene="rpmG"
                     /locus_tag="IWM35_001375"
     CDS             complement(30634..30783)
                     /gene="rpmG"
                     /locus_tag="IWM35_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAP9919235.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            31011..31457
                     /locus_tag="IWM35_001376"
     CDS             31011..31457
                     /locus_tag="IWM35_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HAP9919236.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            complement(31652..33547)
                     /gene="asnB"
                     /locus_tag="IWM35_001377"
     CDS             complement(31652..33547)
                     /gene="asnB"
                     /locus_tag="IWM35_001377"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HAP9919237.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKIDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEEWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
BASE COUNT         9861 a         7097 c         5914 g        10948 t
ORIGIN      
        1 tttaattttt aaataaaaga aatataaata aaaacagagg aagcggaatg atccgcccct
       61 ctgtttttat gattttatta aacttccaat aattcttttt ctttatcagc agcgatctta
      121 tctacttctt tgatgctgtt atctgtcaat gtttgtacgt ctttttctaa tccgcgcaaa
      181 tcatcttcgg taatatcacc atttttttgt tgttttttat attcatcgat cgcatcacga
      241 cggatgttac gaatcgcgat ttttgcattt tctgcttctt ttttaacatc tttagctaat
      301 tcttttctgc gttcttctgt cagttgcggg ataactaagc gaatgacatt tccatcattg
      361 gttggactga ttccgatatc acttgccata atagcttttt caacatcttg aagaatgctc
      421 ttgtcaaaag gtgtgatcat taaaacacgt gcttctggaa tttggattga tgccatttgg
      481 ttcaatggtg ttggcgcacc gtagtagttc acagtaatac ggtccaaaag gctagcgttc
      541 gcacggcctg cgcggatttg cccaagttca cgttgaagat tttgtgctgc ttttttcatt
      601 ttttcttttg cttctgtcat aattgcatct gccattttta tttccccctt acagttgttc
      661 cgatattttc gcctaagatt gcacggcgaa tatttccgtg ttcatttaaa ttgaagacaa
      721 gcagtggaat atcattatcc atgcttagtg aacttgcagt tgaatccatg acttgtaatc
      781 ctttggcaat aacttccaga tgagtcagtt cttcaaattt tactgcattt gcatctactt
      841 tcggatcagc agaataaaca ccgtctacat tgtttttagc catcaggata acatctgcgc
      901 cgatttctgc cgcgcgtaat gctgctgttg tgtctgtcga gaagtacggg ttgccggtac
      961 cgcctgcaaa gataacgatc cgtccttttt ctaaatgacg ttctgcttta cggcgaatat
     1021 atggctctgc gatttgacgc atttcaatcg aagtttgcac gcgtgtcggt acacccacgt
     1081 tttccaacgt atcctgtaaa gctaaggcat tcataactgt agctaacata cccatgtaat
     1141 cagcttgagc acgttccatg cccatttgtg ctccgatttg tccgcgccag atatttccgc
     1201 cgcccacaac gattgccatt tcaacgccca gctcgtgaac ttctttgatt tcttggatga
     1261 tttctttgat tactggtggt ttgattccaa atcctgcgtc cccagctaaa gcttctccac
     1321 tcaattttaa gactacacgc tgatatttcg gtgtgaccat gttcattcct ccaatatttt
     1381 ctattgccat tttaccatac ttagatgatt tcgacactct aagactggtt tttcttgttt
     1441 tgcttaaaaa aagggagcgc acaaaccgtg cgttcccgtc tgttattcaa cagaattatt
     1501 ttttcacttg gctcataact tcttctacaa agttatcttc gcgtttttcg ataccttcgc
     1561 caacttcgaa acgtacgaat gttttaacag ttgcgccttt tgatgcaacg tatttttcaa
     1621 cagtcatatc aggatcttta acgaatggtt ggtccaccaa agcgatttct gctttgaatt
     1681 tcttcaagcg tccttctacc attttttcaa cgatattcgc tggtttgcct tcgttcaatg
     1741 cttgttctgt aagaacagtt ttttcatgtt ctaattcagc ttctggaatt tgtgattcgt
     1801 ttacataacg aggattgata gctgctacgt gcatagctac gtctcttgca actgtttcgt
     1861 cagttgttcc atctagaaca gtcaatacgg caatacgtcc gcccatatgc aagtatccac
     1921 caaatgcagc attgtcgtct ttttctacaa cttcaaagcg acggaagctg attttttctc
     1981 cgataacttg agttgcttcg atcaagtcag attcaattgt acctttatct gttttgattt
     2041 tcattgcagc ttccatgtct gctggtttgt tttcagcaac aagttctgca atttctttta
     2101 ctaaatcttg gaacatttcg tttttagaaa caaagtctgt ttctgagttt acttcaacga
     2161 ttgctgcagt gttacctttc acagcaactg aagccaatcc ttcagctgca atgcggtcat
     2221 ttttcttcgc agcctttgcc attccttttt cacgtaaaag atcaactgct ttttccatgt
     2281 cgccttctac ttcaactaat gcttttttcg catccatcat tcctacgcca gtcatttcgc
     2341 gtagttcttt taccatttta gctgtaacgt ctgccatgtt taactcctcc tgtgtgtttt
     2401 tctttttaaa aaaagctgct tcaaaggaga gtgaggcttg tcgcccagcc tttgaaacag
     2461 ctccatcatt caaaattatt ctgctgaaga attgtcgcct tcaacaacat ctacgatttc
     2521 ttcgattgat gttgctgtgt tttcttctgt gaaatcttct tcaactactt gatcttcacc
     2581 ttggtttcct tcgatgaaag catcagccat ttttgaagta atcaatttaa cggcacgaat
     2641 tgcatcgtcg tttgaaggga ttactacgtc gatttcatct ggatcgcagt ttgtatcaac
     2701 catagctacg attgggatat ttaatttgtg tgcttcttga acagcaatac gttctttacg
     2761 tgggtcaacg atgtacatta catctgggat tcttggcata tcagcgatac cgcctaagaa
     2821 tttttcaaga cgttcacgtt ctttgttcaa accagcaact tcttttttag gtagaacttc
     2881 aaaagttcca tcttcttcca ttgcattgat ttgtttcaaa cggctgatac gtttttggat
     2941 agtatcccag tttgtcaatg ttccgcctaa ccaacgatgg ttaacgaagt attgtcctgc
     3001 acgagttgct tcttctttga ttgcttcttg tgcttgtttt tttgtaccta cgaataatgc
     3061 tacgccgcct tcttcagcaa cgctcttcat gtaatcataa gctgcatcta ctaatttaac
     3121 tgttttttgc aagtcaatga tgtagattcc gtttctttct gtgaagatat atttcttcat
     3181 ttttggattc cagcgacgag tttggtgacc aaagtgtacg ccggcttcta gtaattgttt
     3241 catagaaatt actgccatgt ttgtttcctc cgatttggtt ttatttttcc tcttcttatc
     3301 ttctaccttg ccggcaaact ttttcaagca cctaccgaca atccaataag aatgtggatt
     3361 tgctgatgca taaagcaccc tcagctatta tacagacaaa agatccgtat cgcaagagca
     3421 aatatacttt tctgctcaac attcaatagt ttacctgaaa gataaaaaga attcaagaaa
     3481 aacagtagat ttctctttgt ttctttatta tcctttttcc ataaaataat tttaggtata
     3541 aaagacgaat attattgggc aatccttctt ttttttgtta tgatacatgt gaaatctttc
     3601 gtaaaggaga aagaaaatgc gcaaagctca aagacatttg ctgattaaac aagtcattga
     3661 agaattcccg atccgtacgc aagaagaact attaaacaaa cttgggcatt atggcgttac
     3721 cgccacgcag gcaacgattt ctcgagatat ccgtgatcta aaaatcgtga aggcacctga
     3781 tgaaaatggc gtttcccgct tcgtattgtt tcgcgaaaaa ggaactcctg agactaggac
     3841 tgaggaagag gatcggctgg ttcaaatgat tgaagacatt gtattgaaag tcgaacgagt
     3901 ccactttttg acgatcgtca ataccttgcc agacaatgct catttatttg catctgtttt
     3961 agatgagatc aaacctccct ctattgtcag tacaattgct ggattcgata cgatcatggt
     4021 catctcggaa aatgaggagg acgcaaaacg agtagaagaa tttcttcacc atccaactga
     4081 agtatcactt ttttaatcag agcacgtatt cctaatatgg aatgcgtgtt tttttatttt
     4141 caatattttc cttattgaat aaacctttac actctttaca aaaatacatc attttttatt
     4201 caaatttttc aacaccttga ttgaatcaca ataatcatca tttatattgg atgttaacaa
     4261 gagcagcttc ttgctctttg tgtatttcaa aaaagaaatg aaaggaaatc atatgtataa
     4321 attattaact tctggaccat tgacgacttc tgaaacagtg aaaaatgcta tgctgatcga
     4381 tcattgtact tgggatcaag aatacaaaga aattacacaa tggataagaa aaaaattatt
     4441 ggatcttgct caagtatcgg aaggaatata cacaacggtc ttaatgcaag gtagcagctc
     4501 cttcggggta gaatcggtat taagttctgt gatcaggcca gaagatactg tactgatttg
     4561 ttcaaacggc gcatatagac aacgaatgat caacatgtgc gaatgtctga aactgaacta
     4621 taccgtttat gaggtatcgg aacatcaaat cctccaagct catgtgatta cacagctgat
     4681 tgaagagaat ccgtctatct ccgcactagc aatggtccat agcgagacta cttccggtat
     4741 tttgaataat ttggaagaga ttgctgctgt cacacaagaa agagatcttt tgtttatagt
     4801 ggacgcgatg tcaagctttg gtgggatacc gatttgtgta gccgaactag ggattgattt
     4861 tttgatttcc agctcaaaca aatgtattca aggtgttcca ggcttttctt ttgtacttgc
     4921 taataaagcg gaactggaaa aacgaaaaaa tgttagccga agtttgtcac ttgatctata
     4981 tgaccagtac cgcgtgatgg aaaacgagaa tggcaaatgg cgtttcactt cccctaccca
     5041 tacggttttg gcttttcaac aagcgctgaa ggaactagag gcagaaggag gaatcgaaac
     5101 tcgatattca cgttatctcg ccaataatca tgtgctacga caaaaaatga cagagatggg
     5161 gttccagcct ttcattcaag aatatcaggg gccattcatc acctcttttc tttaccctaa
     5221 ggactggcga tttgatttta atcattttta tcagtattta aagcaaaatg gatttgtgat
     5281 ctatcccggg aaaatcagcg atcatgaatg ttttcgtatc ggaaatattg gcgagatcta
     5341 tccggaagat attttggaac tgacagcaat tatcaaagac tataaggaga acgttaatga
     5401 ttaaaactgt aatttttgat tgggcaggaa caactgtgga ctttggctgt atggctcctg
     5461 tccacgcttt tagaaatgct tttctagaaa aaggaatcca actgactgac aaagaaatcc
     5521 gctaaccgat gggaaaatta aaatgggatc atatccaaca gcttctttcc ctcccttctg
     5581 tcaaaaaaca atggatagct atttatggac aactacctca ggaaaaagag gtggaggaat
     5641 tgtatcaaca atttgaacat tatctgttta aagatttggt caagcattct accttgaaac
     5701 ccgacacatt ggaaacgatc gaacggttga gaaacgctgg gatcaatatt ggttccacga
     5761 atggatttaa tgctcctatg atgacaatcg tagcagaaac cgcaaaaaaa gaaaggtatt
     5821 caccagactt catggcaact tttgaagacg tcgaagggta tggtcgaccg tttccttata
     5881 tgatccataa aaacatgcag tacttcaaaa acaagtcggt tgatgaagta atcaaagtcg
     5941 gagatacggt ctcagatatc caggaaggga aaaatgccgg agtgttgaca gtaggtgtaa
     6001 ttgaaggtag ttcgctaatg gggatttctt atgatgagta ccaaaaatta gagatgggga
     6061 aaaagatcgt ttaaaacagt ctgttgctct tagattccaa caagccggcg ctgatatttg
     6121 tgtaaacaat ttaaaagagt taacagaata cattttgtac gctgacatga atccagaatg
     6181 acttttatac aaaataagag gttgtgaaaa agctgtcctg tatcaagtaa taaaaacagc
     6241 caagcaaaaa tagctcctca tatttttcgt ttggttaggc ttattaccgc agatgcaagc
     6301 ttttgaacac cgtttattcg aaaaaaaaaa gggagctgtg acacgacttt tgtcacagtc
     6361 cccttatttg caaacaaaca gtgtttaaga gacaacctct ccactaaacg gtgttcattc
     6421 agctgactct cggtatttgc tcaaaaaacg ataaaatttg aaaagctatt ttctcttttg
     6481 tttctctaat actcggatca gaacgctgat ttcacattct ctttttctta catcttatct
     6541 ggtgcatgaa gtcctagcaa acgaaggtct tctttcaata atacagtcac tgcataaact
     6601 agagctaagc gtgcttcttt ttgttcatca tcagctaaaa tcttcgtatg agcatagtat
     6661 ttgttgaatg cttgtgctaa tttgattgca tgtttcgcaa tcactgacgg ttcgtatttt
     6721 tccccagcag acaagaccgt ttcagggtat ttttgaacta atttaaccac ttcccagctg
     6781 gtatcgtcat tcaatgcata gtctgctgtt tctgaaggga caaatcctgc tttttccaac
     6841 aggctgactg cacgagcatg tgtatattgc acataaggtc cagtttctcc ttcaaatcga
     6901 acaacttctt ccaggttaaa gtcaaatgta ttcagtcgat cgtttttcaa atcgtggaaa
     6961 atgactgccc caaccccaac ttgtttagct acagcatctt tgttttccaa atcagggttt
     7021 ttctcgctga tttgttcttt agcagattgg attgcttcat ttaatacttc ttctaataag
     7081 acgatctttc ctttacgagt tgataatttt ttaccacctt gcgtgatcaa accaaatgga
     7141 atatggtgca tgtcgtcaga ccagtcaaag cccatttcct tcaagacagc tttcaactgt
     7201 ttaaaatgat agctttgttc attccctact acatatagag attgtttgaa gtcgtaagta
     7261 cgtttacggt acaatgcagc agctaagtca cgtgtaatgt atagtgtcgc accgtctgac
     7321 ttcttaatca acgctggatt caggtcataa gcagataggt caacgatctc tgcaccttta
     7381 tcctcgttca gcaaatgttt ttcttctaaa agtttgacga tttcatccat cttgtcattg
     7441 tagaaagctt caccgttcaa cgaatcgaaa cgtacttcca gcagatcata gattttattg
     7501 aattctttca tcgattcatc acggaaccat tgccataatt gaatagcctc tttatcgcct
     7561 tcttccagac gtttgaacca agcacgagct tcttcattta attctggttc tgtttcagct
     7621 acttcatgga attggacgta taaacgaagc aattcattga ttggttctgc tttaacagct
     7681 tcttctgtac cccatttttt gtaagcgacg atcagttttc caaactgtgt tccccagtca
     7741 cctaaatggt tgatgcggat tggctgataa ccgatttttt ccataatgaa accgatcgaa
     7801 ttaccgataa ctgtcgaacg caaatgtccc attgagattg gtttggcaat attaggagaa
     7861 gacatgtcga ttggtactgt tccttgattc ccgatgttgc tatccccata atgttctttt
     7921 tctttcacta ctgtttgcaa aacttttttg ctgataagtt ctttattcat gaaaaagttc
     7981 aagtaaggtc cgacaacttc gattttttca aagttggcag aatcgatttt ttctgctaaa
     8041 tctgcagcga tttgctgtgg cgcttttcgg taaacttttg ctaatgaaaa ggctggaaat
     8101 gcaacatctc cgtgttcagc tgattttgga ttttctagta attgctcgac ttgttccagt
     8161 gttaaatcat ctttgacagc gtcatagaca gcttttgcga caagatcttt attattcatc
     8221 tttattcctc ctaaagtgtt cccttgattg ttaaacaaaa aaaatctcta gcgacggcat
     8281 acttagtatg ccatcactag agacgagatt tcccgcggta ccactccaat tgtcctaaaa
     8341 aaggaccagc tccatctgat atcggtagac gaccgtctgt ttaaaacaga attctccaag
     8401 tgcgcttcgt tgtatcttct tcattcggct tccaccactt ccgaattcgc tagccaagaa
     8461 tgatacaagt actctcttgt tcaacaaatc ttagtcttat taaactttct tgaattatat
     8521 cagaaaaaaa aacgaaaggc tagtcgtttc ataaaaaaac aatccagatg gcctaaagga
     8581 gaagaaatac cacctggata gctataaatc atttcgtaaa tacgaaagac ttaacaaatt
     8641 aatcagctac gataatcgtt ccttcttcag aagctaaaag attctcgata ttttctaaag
     8701 aagtgatgat tgccttagcg tttggttttg cttcaacaaa tgcaatagct gcttctactt
     8761 tcggaagcat gcttcctgga gcgaattgct tttcatcaat gtattgtttc atttctgaaa
     8821 cagtaacagt ttctaatttc ttttgatctg gtttttggta attgacatac acattgtcta
     8881 ctcctgtcaa tacgatcagc aaatccgcat cgatgatttc ggctaatttt tctgaagcaa
     8941 aatctttatc gattacggct tctcgtcctt ctaatccagt agctgtttct acaacaggaa
     9001 tcccaccgcc accaacagag actgtgataa ctccttgatc gaccagcgtt tcgatgacac
     9061 gtgcttcttt aatcgagatg ggtttagggg aagcgactac ttttctccat ccacgtccag
     9121 catcttcttt gaatgtcaca gtggagtcag cattcatttg ttctttggct tcttcttccg
     9181 tgtagaatgg accgactggt ttacttggat ttttgaatga aggatcattt tcatccacaa
     9241 taacttgagt gactaaagaa accacatctt tatcgatccc tttttccttc agtacttcgc
     9301 ccatggcgtt ttgtaaccag taaccgatag atccttctgt cattgcaaca catgtatcta
     9361 aaggcattgc cggtgttttc tcagaatcag cagcttgctg ctgaatcaat aggttaccga
     9421 cttgcggacc atttccgtgg gagatgatca attcatctcc ttgttcgata aatttcacta
     9481 aatactttgc tgtttccatt aacgcttctt gttgggcttt tgcacttgca tccgtagaca
     9541 agatggcatt gccccctaaa gcgacaacta cttttcgttt ggccatttca ttcgtccttt
     9601 cttcagttct accgaatttt ctagataaca aaacgagcca gcgttttgtg attttccctc
     9661 aatgctgact cgttttttcg tgagatgaat gtggttatgc ttaaacgcga gggataaaca
     9721 agttacctaa tgttgctgcc atgatcgctt tgattgagtg catacgattt tctgcttggt
     9781 caaattgacg agcatatttg ctgcggaaga cttcatctgt tacttccatt tcagtgatgc
     9841 caaagcgttc tttcatttgt tcaccgtaaa cagtgtttgt atcatggaaa gctggcaagc
     9901 agtgcaagaa gatcaaacga tctgtgttgt gtgttttttc taccatttcc atattgattt
     9961 ggtaaggttt caacaatttg atacgttctt cgaatttatc ttcttcaccc attgataccc
    10021 atacatcaga ataaaggacg tctgctccgt ctacaccttt atcaacatcg tcggtaatca
    10081 tcaattgtgc accggatttt tcagcaaagc cttctgccat tttgacgatt tcttcttcag
    10141 gttgtaattc ttttggtgca acgatacgca tattcgctcc taaaattgca cctgtgacta
    10201 ataaagagtt agccatattg tttcgaccat ctccgcagta agcaacagtg ataccttcta
    10261 ctgttccaaa gttttcttgg atagttaaaa agtcagcgat catttgtgtt ggatgccatt
    10321 catcagtcaa gccattccaa actggaacac cagagaattc tgctagttcc tctaccattt
    10381 tttgactgaa tccacggaat tcgattccgt caaacatacg tcctagtact ttagctgtat
    10441 cttctgtgga ttctttttta cctaattgga tatcatttgc acctagatat tcaggatgtg
    10501 cacctagatc gatcgctgca gtagtaaatg cagaacgagt acgtgtagat gttttttcaa
    10561 ataaaagcgc aatatttttt ccttctagat agtgatgtgg gataccacgt tttttcaagt
    10621 ctttcaagtg ttctgaaaaa tcgatcaaat attgtaattc ttcttttgta aagtcttttt
    10681 ctgctaataa gcttcttcct tggaatactg attctttcat attaaacatt ctcctttaga
    10741 ttgtttttat ttaagattaa agaggaaatc ccgacggcag ctgacagtaa gactgccgtc
    10801 aggaaacaaa ttacttcaag tcttcacgga caagtggttg gctcatgcag cgtggaccac
    10861 cacgacctcg tgacaattca cttgaattga tttctaacac tttgatgcca tagctacgaa
    10921 gcaattcgtt cgatacataa tttcggttat aagtgactac aacacctgga gcgattgcca
    10981 atgtgtttga tccatcattc cattgttctc gaggtgcaac gatctcatct ccattaccag
    11041 ttgggatcag tacaagatca tctaatccaa gagcagcttt catcgtttcg tgtaagttat
    11101 ctgaatgagt aatcttgata tcatcaccat ctggttcgat tgtaaagaca tccatcttac
    11161 cgtctttact ttgaattgct ggatggatag tgaatttatc gtgatctacc attgtaaata
    11221 cggtatccaa atgcatcatt gcacggttgt tcggaatttt gattgccaac actttttcaa
    11281 atcctgaatt ttttgcgaat aggtggcgtg ctaatttttc taaagctttc gcattagttc
    11341 gttgagaaat cccaacagct actactttat cgcttaagat caattcgtct ccgccttcga
    11401 tatgatctgg attttcacga tccaaccata cttcgattcc tttatttgca aatcttggat
    11461 gatgtttcaa aatatattct gtaaacatcg attcacgacg acgtgcttca aaagtcatct
    11521 tattgactgt catcccattt cccattgatg cggaagggtc gcgtgtaaag tacaagtttg
    11581 gcattgggtc catgtaaaat ggatagtcat cgtctgctga cagatcatat agactgctag
    11641 aacgtacttc gatatctttt gtacgaaccc cagccatgat tttatctacc atttcttgcg
    11701 tttccattga tagaaggaac tcatgaagac cctctcttac agcattcgat gcaatatgag
    11761 attcatccaa cattttattc aagaattgtt ctttgacatt gcctgcgtca atcgcttctg
    11821 ccgctaattt ttccaagtaa agagtctcga cgccttcatt ttgcaatgtc ttcgcaaagt
    11881 tgtcatgttc ttcttgagca atcggaagat atggaatatc atcgaacaac agacgatcca
    11941 taatatctgg tgtcaaattt tccacttctt gtccaggccg tttcagtaaa actgtcttta
    12001 atttccctat ttcagagaaa acgtgaatag gtttatccat gttgtgttgc ctccttcttt
    12061 ttgattacat gtataggata tcctcctttt gtaatcactt tcataatttt gtctcattca
    12121 aaatgagaca ttcctcacat taatattcga taattaaata attaaacgtt tttctatttt
    12181 taagcgtaat ttattcattt tttattctgt atacactttc aaaaaaatat ttattcattg
    12241 aaaaaatttt tttgaataaa ttaaaaaaat aaattatatt atcgttgtta gcgtttacta
    12301 tatgtattat tgttgttttt tatacacaaa tcattcgctt caaaacaaat tattcaatac
    12361 cattgtatat tttttacata ataacaaatt atatttgtaa agtattaaaa cttgtttcta
    12421 atgttggtat gttaaaactt tacaaattct ttaaaaaaag tgacaataaa tatagtgaac
    12481 aataaggaga ggacttttta atgcgtgtta ataaaatcga ttttcacttt tcaaaactca
    12541 gatatcaacc tgattttgaa catttctcgg atgaagagtt ccaacagatc aaggaacata
    12601 ctgttcttcg ttcttataaa aaaggacaga tccttttcga cgatggtgat cccagacaac
    12661 ggttgtatat cttgaccaaa ggactcgttc gtttagaacg ttatgatgaa agtgcaactt
    12721 attactacta tgactatgtg aatacaaata acctttttcc tatgtcagga atgtttggag
    12781 aagagaccta cacttataca gctcaagcga tgactgatat tgaaacattc taccttcctg
    12841 tttcctacta tgaaaaatta gcacgaaaaa atcccgaaca gttgatctat atgatacaaa
    12901 aaatttctaa agtagttgaa atacatgaat tgcgtatcca aatcggattg acctctagcg
    12961 cctttgaccg ggtaaaacaa aatttattca ttttgaaaga agaattgggc gtaccaacaa
    13021 aagatggtca tatcaccatt ccttatccga tcaccttaaa agaattagca gtgaatagcg
    13081 gcacgacacg tgaaactgct ggacaagtaa tcaaacaact acgtgattcc gggctgttag
    13141 attatgacaa aaagcgtttt actttctgta agacagctac tactccagct atgagagaat
    13201 aacgcgaaca aaaataagca gtgaccctga taaatatcag ggtcactgct tattttcctc
    13261 cctaaaactt ctgaaagatc cggcagtttt agatcactgg acaagactca aagattttct
    13321 atgtgtgttt cagttatgta atattcactg tttgcaaaaa atactgttag attaactaga
    13381 tgaagcgtct tgtcctcatc cgatctagat tattcgatat tcctcgacaa taaacgatta
    13441 ctcagtgagg gcaatcagag gaacggataa tgataaataa gctgtaaaaa atagcaaaat
    13501 aacaataatg attaaaagta tgtaaaaaat agggctttga caaaaacgtg tcacagccct
    13561 atttcgatta aacggtgtgt acctgtcaac tcttcggtca caagtcataa tgttcataaa
    13621 catgaggagc tgttttttga aattgttcct tgtgaatcag agttaaacga caggcttacg
    13681 aactcttgat taaacggcgt tcaaaagctt gcatacgcgg taataagaac agccaggtca
    13741 aaaacatgag aagccgtttt tcctgggctg ttcttattac ttgatgcagg gcagcttttt
    13801 cacaacctct ttattcagat aaaaccaagc ttggatcagc attcaactga tagctagcaa
    13861 aatttctttt ttgcatttcc acatgtgctg ctgctccaat cattgcagca ttatctccac
    13921 aaagtcgaag agggggaatc actagttcta cctcagggag tttagctgcc aaagcatcct
    13981 gcaatccttc acgcaaccct tgattggccg ctacacctcc agctataatc agttgcttga
    14041 ctggataatt ttgacaagct cgaagtgttt tagtaatcaa tacatcgatc acacttgctt
    14101 gaaagctagc tgctaaatca tttttgtcta gatcttctcc acgctgttgt gcattatgca
    14161 ccaaattgat aaatgcactt tttaatccgc taaaactgaa atcataatta tcttcatgga
    14221 tcatcgctcg cggaaaatgg taattatctt ttccctgatg agctagctga tcgatttctt
    14281 ttccgcttgg gtaagaaaga cctaatactc ttccaacttt gtcataagct tctcctgctg
    14341 cgtcatctct tgtctcccca atgatttcat atgatccatc ttcttgcata tagactaatt
    14401 ctgtatgtcc tccgctaaca agaagtgcca tcaatggaaa ctggaatggt ttaaccagac
    14461 gggctgcata gatatgccct gccatatggt ttacaggaat aagcggcaat tggtgtgccc
    14521 aagcaaaagc cttagctgca gaaataccaa tcagcaacga gccgacaagt cctggtccat
    14581 aagtgacagc gactgccgat aaatcttctg ccgacacacc agcttctacc agtgcatctt
    14641 ccaagcataa ggtgatttgt tctacgtgat ggcggctagc tacttcaggt actacgccac
    14701 caaaacgttt atgactgttt atttgagaag caacgatgtt agataagatc tctgttccat
    14761 ttctaacgac cgctacactc gtttcatcac aacttgattc aatcgccaga atcaattctt
    14821 cattcatttc atcgtctccg tttttaactt tctgctcata atgatcgcat cttccgtcgg
    14881 attgcgataa tatgatttcc gtttacctaa tatacgaaac ttttctgatt cataaagttt
    14941 tcttgctgct tcattagata gacgtacttc taaaaaaaca tgcagcgaat cttttatttg
    15001 ttccgtaatc agtaattgta acaaatgccg tgcatgccct tttccttgtt ctgaaactgc
    15061 aacagcaata tttgtgatct cgacttcatc tattactttc gagtatccaa taaagccaca
    15121 gatctggtta tcttcagcta aaagcagata atcagagtga ggctgttgga tgtctgccaa
    15181 aaactgcatt ttcgtccaag gtgaaccatg tggataagca ttttcgctaa tcatccatac
    15241 gcattctgcc aagtacgctt catcaaaatc tttttttctg caaatcaact aatctcactc
    15301 attttctaaa agttttatca tatccaacgc atcttctttt gtttcagtgt aatatctttt
    15361 tttgatcgtt gttgcttgaa accccaattt ccgataaagt ctttgtgcat cttgattact
    15421 catacgcact tctaatgaca tcgtctcaca gcgattcatt atagcaaagt tctcgatttc
    15481 gtcaataagc agcgaaccga ttcgtttacg ctgatatgcc ggtcgaacgg caatattcgt
    15541 gatatggcaa tctaaaccga acgctcttga accgataaaa ccaatcattc ttccattatc
    15601 aaagatacct agatatagat gaggaagcgg tgacttcaat tctgatataa aggcactttt
    15661 tgtccatggc aattcgccat tatagacttc tcgttcgaca tccagcagct ctttgatatc
    15721 ttctgtagtc aattctctta gtacgtaacg acgatcgatt tgttcgactg ttttttctgt
    15781 ataaatcatt ttatcttgaa aaaagcttct ggcatacgct ctaaattttt tcaacataac
    15841 tttcgtctcc aggtgtgtgc gtctccaacc atttttcttc tgcttctact cgttttaaat
    15901 agcgaggaag aaaagcttgt ttctctgcaa ctggttcaac taaagcacct aattgagcca
    15961 cagttacacc acttggaata tcccatgctg gaactgtatt aatcatcgca ctaggcaata
    16021 tttcctttat ttcagagaag aatttgtgac aatcactccc gacgaaataa atagcttcat
    16081 tcagcttttg tagttgtgtg aacaaatcag aagcagaaat atgtacatct tcaagtacag
    16141 tttctaatct attttctttc cagcggtatg ccccagcgta tacatttttt cttcgagcat
    16201 caaacatggg cacaataata ttatccacac cgatgcaatt agccgcgatg gttttcagac
    16261 tagatactcc tactagctct ttatctaatg tataagcaat cgtttttgct gtcgttacac
    16321 ctatacgaag ccctgtataa gaaccgggac catcagaaac cgcgattcgg tcaatgtctt
    16381 ttggacttat ttgaagatct gcaaacaatt ggtcgattgc aggcatcagt gttacactgt
    16441 ggttgcgttt gatatttgtt tgtatttgtc caagaatttg ttcgtcttct accacaccga
    16501 tcgccaaagt ttggtttgca gtatctattc ctaatgtaat catatatttg atcctttctg
    16561 ctatgacatt ctcgccttcc attgtagcat acttttttat tctgaggatg aaaataccaa
    16621 aggcaaaact agtacaataa tacatgatta tctgctggcg ggcaaatttt gccccttcct
    16681 gaaacacaaa aaagctgcgg tatcactcca ttttcccaca gcttttccat cttttatttc
    16741 gttgattctc gaataatcaa ctctgtttcc acaataatct ttcttgaaac cggtgctttg
    16801 gaattactta attgaagaat cgtctccact gctagctctc ccatccattc ggtataaact
    16861 tttacagtag aaagcgaggg cgttacatat ttcgctacac tgacatcatt gaagcccatc
    16921 acagaaatat cttctggtac cactaaacct ttttcttgaa tcgctttcag tgcgccaatc
    16981 gctaatgcat cattagctgc aaaaagcgca tcaggaacat gcggattcgt ttttaaatat
    17041 tgcttcactg cttgatatcc tgactcgaca gaaaaatctg cttcgataat tggaccttcg
    17101 tcgtagcctt tttctgccaa aacttgttga aatgccataa ttctgggatc ttttagccgc
    17161 tgatggtttt tcttcgtata ttctgttcca gcaagcatag cgatatgttg cgctttatgg
    17221 gagacaaagt gttcaatgac tttttgaaca cttgacttaa agtcgacaac tagagagtcg
    17281 attccttgag aagtgccgtc agaatctacc aaaagcaagg cattttggga tcctttcatt
    17341 tctaataact gagcttcgtc gaattttcct agtgcgatcg ttccatctgc cggaagatca
    17401 ctaatggaat cccacgtttc tttcaataaa gtaacgtttg cttcttctgc ctttttttcg
    17461 attcctaggc gaatagataa ataataaaga tcttcaagct cttccgtatc gtcgtaccac
    17521 tggatcaggc gaagcgtttg cttagtattt gctgcatttt tacgatgttt cgtataattt
    17581 aagcgttctg ctgcttcaaa aattttttgc tttgtggcta agccaacgga aagatcagga
    17641 tcatagttga gaactcttga aacagtagca ggagaaactc ctgccaactg cgcaatgtct
    17701 ttaattgttg ccatttgtct ctcctccttt tttacgttta taaaatcgca gtaaatcgtt
    17761 caaacgctgc ctgtcctttt tcatctcgct tgaatactcc agcgtcttcc agtacacgtg
    17821 caaaaacctg acctacagat ttttgtacga catcggtgac cgtatctttc gtcaaatcag
    17881 gatgttcttt ttttaattgt tctgcccacg gacgatgata ttcagccatc tggttttctt
    17941 ctcctaataa gaaatgctct acttctacta gttctggttt caaacgaggt ggtaaaacag
    18001 caagccccat tacttcgatc aacccaatgt tctccttttt gatatgttgg acatctttat
    18061 gtggatgaaa aatcccatca ggatgttctt ctgaaacatt gttgtctcga aggacaaggt
    18121 ccaactcaaa gtaattgtcc cttcttcgtg caattggcgt aatcgtatgg tgtggtgtac
    18181 catctgctga aaaagctctg atagatactg tttcatccga atatgctttc catttttcca
    18241 atactgagtt agctgcttca ataagcaagt ttttctctgt tgattgcaaa cggatcacag
    18301 acatcggcca tttcacgact cctgcgatga cttgcggata atcttctagt ttgataagtt
    18361 tttctatcgg tgcttttgcc ataggaaatt catgacgtcc tgcctgataa tgatcatgag
    18421 aaagaatcga tcctccaaca attggtaagt cagcattcga accaacaaag taatgaggca
    18481 agacttcagt aatccgaagc agtctagcaa atgtttcctt tgtgattttc atcgggcggt
    18541 gttgttctga taaaatgatc gaatgttcat tgtaataagc ataaggggaa tattggaatc
    18601 cccagctttc accgtctaga ttcattcgaa taatccggtg attggtgcgg gctggatagt
    18661 tcaagcgacc tctatatcct tcgttttcca tgcaaagcat gcaggtagga tagtctactt
    18721 ttaccgcctc gcgttctgct gcgatttgtt ttggatcttt ttctggctta gataaattga
    18781 ttgtgatttc cagttcacca tactccgact ctgccggaaa tttgatgttt tttgcgatgg
    18841 ctcttgtttt gatgtagtca tttttcttac ttaaattaaa gaaatactca gttgcttcct
    18901 ctgggctttt cgaatagtgc tgtgcaaaga atgcattgac gactgaaggt ggcggtgtga
    18961 gaaaatccat taattgcgct tcaaaaatct cttgttctgc tggcaattct gaaagaacac
    19021 cattttctct tgccttagcg acaagctgat ccaataagtc tacagattct tccttaactg
    19081 gtcttacttc aatgctttct agagcatctt ctcctaccat ccctaaaaga cgattctgta
    19141 aatataagcg gtccatttcc atccatccgc cagcttcgat cgcaagtgtg gcaaaatcac
    19201 aaatcgtttg actgattgac atatctcatt cccctattct tcataaccat ttggatggct
    19261 gacatgccaa tcccatgctg ttttgataat cgctttgata tctgtgtatt caggtttcca
    19321 gcccagaatc tctctcgctt tttcacttga agcgactaat cggctaggat ctccagctct
    19381 tcttggtgct atttctgctg gaatttcttt tcctgtcact tctctagctg cttctagcat
    19441 ttccttgact gagtatcctt tgctgctgcc taaattgaag aagttgcttt catttccttc
    19501 tttcaagtat tccaaagcta aaatatgtgc agcaatcaaa tcttctactt gaacataatc
    19561 tctgatacat gttccatctg gtgtatcata atcgtctcca taaacagcca aggcttttct
    19621 ttgaccaaga gctacttgta aaatgattgg gacaagatgc gtttctggtg tatgatcttc
    19681 tccgatagat gcatctgctt ttgctccggc tacattgaag tagcgcaaag cgacatagcg
    19741 catgccatat gcttggtcac accacttcat cattttttcc atcatcaatt tactttcacc
    19801 atacggattt ttaggattag ctggtgtatc ttctgtgatt ggagactctt tcggctctcc
    19861 ataagttgct gcagtagaag agaatacgat tttattcacg ttgaactcgt gcatgacttc
    19921 aagtaagatt tgcataccgt atacgttgtt gttgaaatac atcaatggtt tttcgacaga
    19981 ttctcctact aaagagcttg cagcaaaatg gattactcct tcaatcggtt ccttttcaaa
    20041 tacggaacgt agaaattctt tatcccggat gtctccttca taaaaatgag catctggatg
    20101 tactgcttgt ttgtgacctg tgagcaaatt atctaccaca gctacttgat accccttttg
    20161 aactagctgg tctacagcgt gcgaaccgat ataaccggct ccgcctagta ctaaaattgt
    20221 cataaaaaac actcctttga ttagtaaaat tgtgtttgtt gattctatat aatttaaaaa
    20281 acgaagttta cttttatggt gaaaacaaac aaagtaaaat ttcgtaaatt catatggtaa
    20341 atttataata aaacatttag taaacatttt caagggtttt ttgcaaaggt ttacaaacaa
    20401 acgtaaaact agcctttttt tctcttttgt tccataatag atataacaaa taataaatga
    20461 ggtggattca atgaaaaaat ttgtatctgg aatattagta ggaagtctgg caacagccgc
    20521 tgccgttgct ggtttggttg cttctgtgaa aaaaacagtg atcgatccaa ttgacgaaaa
    20581 agaagcaatg atcgaagaaa accgtaaaaa agcaatgcgt aaacgcgttt ctcgttaata
    20641 attatttaaa aatacgcatg aagactttct aaaaaacaga taagtcatca tgcgtatttt
    20701 tatttgtcag tcaatataag ttgtgctatt ttatctgcct gtctcagatt ccatggatct
    20761 gtataaggtc tagctactgc tgcagttagc caatccacac cttcaacagg tattccatag
    20821 caataaaagt cagactggat ttcccctgaa gcatcaatca cgcgattcgt acctcgctca
    20881 acatctatag cttctgaatg ataacttcct gtattatctg aaaatgaaaa agggcgaaca
    20941 agtttgtttt ccttcaaatt ttttatgaca ggattacttg aacgagaaaa gcttgtttgt
    21001 ggcaatcgag cttcaatcaa atagcgactg ctgatttctt tatcttgtag agaaataaca
    21061 tagcggttgt gcttttgctg aatatcaaat ggcggttcga tcaaatgaaa aatacccgca
    21121 tctatcagtg ctgctaattc tttcgtccga atgattggcg gaccgatcgt taaaaagcta
    21181 tttagatgag taaaccatcc ccatagtatt tctttgtatt ccttagcaga aaaaacgtgc
    21241 cattcaatag cctgccggat tggatcacgc cagtctttta atgcatcaaa tgttgaagca
    21301 aatgctcctg tgcaattgcc tttttccgct tcttttatct gcccttcaat aaatattcgg
    21361 ctagcttctg aaaagggctc attctgatag gcaagaggag tttctaaata cgaccaatcc
    21421 catctataag ggattagtgc tggatgcttt ccgagacact cctctgaaga gagggagaga
    21481 aaatcatgct caaaattttt ctttggaatc ggcaagtgat attcttcgat gattttcttg
    21541 taataaaaca actcaacgtc ttttttcaga tagtcaaaaa atgtttcgcc agtcaaaaat
    21601 cccgactgat gccattgctc caggttttct tttgttagta accgtggcca tgccattgct
    21661 ccgccttgct tttgatttct tcctcgaggg taataaggga gcccccttct tgatccactg
    21721 tatacaatcg gttccttgcc tgatggatga tagaccagtt ttcccttttc gttctcgaag
    21781 cgccctcctc ttgatactgt aaacaatccg acataatcaa aaaaacttaa gcctagacct
    21841 cttaaaaaga cattttcttt aggcggtatc ttcgaaagat ctacatctgc aggattagaa
    21901 ggaagctgat acagtaaaac atttttcttt gcgtagtcta cccacactct ttcttcttct
    21961 gtctcatcat tttcccagtg accgcttgct aaaacaagct gatcgataga atagctttgc
    22021 tgatctgtct gtaaaagata accatctttt cttttgctta ctttttttac taaagaataa
    22081 acaaactgaa tagaagctgg aaattcttct tgcaactttg agaaaaacca gcgttgatag
    22141 agtccgtata aacatcgagt agattgctga tctttttcca gacgattgat ttcttctata
    22201 attagttgct tatttttggt taccgtctgt tcgatataat ctgtagcagg tcctttactc
    22261 cattgaaaca agttgggtcc ctgtgttacc aatcctccac tggacaatgt ttcatctgta
    22321 aaaagcgtca cttgttgaga aacagaattc attaataact ccggtggctg attcaaccgc
    22381 caaactcgac ctccaggacc attgggatcg aaaagaaaaa gttcaagaga atgtctgtcc
    22441 tgattattat ggagaatcct ttctgctaca cttaatcctc taggtccggc accaatgatc
    22501 cctattttca tttgcatgcc tccatttcga taaatttttt caataaaaca gcatggttct
    22561 gatgttcatt ttttgcacca taaacaaaag taatgactgg atgttctttc ataagttgac
    22621 tgagtttaaa aatagcttgc ttcttttctt cttcgaaaat caattcttgc tggtatttct
    22681 ttgcaaatga agcaaatcgt tctggtacat ggtcaaaagt tttgcgtgct tcattactag
    22741 gtgcaatttc tttcaaccac agatctaact gcgctttttc ttttgaaatt cctcgtggcc
    22801 agaatctatc cacgagtatt ctatagccat cctctttttc agcatcaagg taaacacgtt
    22861 ttaatttgat catagttgtt tccttctttc taccttttcg cttagcttat atatgtagat
    22921 aatagcacaa aataaaaaaa atgaacggca caactatcca ttgcgccgtt catgtgatct
    22981 tggtcaaaat ctaatgattg attcggtttt taaagctgtc atacaatcat ctgttttata
    23041 cttttttact tttgattttt ccagaccatc cgtcatatcc acccttaagt aaataaatct
    23101 cttgataacc tgctttacgt aatttatttg ctgtacggat gctcaaactt ttcttttgat
    23161 cgtaaatata gacaggctgg tcttttcgaa tagatcctaa tgaatctttc aaaacagtgt
    23221 aagggatgtt tcttgcacct aaaatatgcc cagcatcaaa cacatctttt tcgcgaacat
    23281 cgattacttg tgcttttctc attccttctc gaaattcttc ttctgtcaaa gtcgttgctg
    23341 aacgtttggc cattactcgt agatagactt cccaaaggat gattgcaagc acaatcaata
    23401 gcaaaatgat attaattacc cataaaacca tgtaatgcct ccgttttttt aagcaaattt
    23461 caaggctaat gaggcagcac caatgacacc agcttcatta ccaagctctg ctaatttgat
    23521 ttttgttgat tctcttactt gcgggaaagt aaattcttta aaatatttct ctacacggct
    23581 acgtaaaaat tctcctgctg ccgatactcc tccacctaac acgatacttg acggatttaa
    23641 tgtattcccc aagtttccgc aagctaaacc taagtagaag catacgcgat caacaaccat
    23701 caatgcaaac ggatcgtctt cttgagccat ttcaaagata tctttacttg ttacttcttc
    23761 tccgttgtct agcattgctt tcagttttga atcccctgcg tattcttctg ccaagaaacg
    23821 acctaggcgt accacacctg tagcgctaga tacagtttct aaacatccgc gtttaccgca
    23881 agtacattcg aacccttcag gatccacagt gatatgtccg atttctcctc cagctccgct
    23941 cacgccgtga atcaaatttc cgccagcaac gatacctccg ccaacacctg ttcctagagt
    24001 aacgaataca acgtctggat cgttttctcc agcacctttc catcgttcac ctaaagcagc
    24061 aacattcgcg tcattatcga tagcgaattt gattcctgtt cctgcctcga tcagtttttt
    24121 cacaaattga acttctgtcc agtttaagtt atacgcacca ataaccgttc cagcttcgct
    24181 gtccacgctt cctggggtac ccatgccgat tccgataaag tcttcggctt tcatattata
    24241 taaattcaaa cgatgattga ttgattcaat gatttcagga acgatatgct taccatcatc
    24301 caaaatattg gtatcgatgc tccatttttg ctgaatctct ccttctggtg ttaaaattgc
    24361 aaattttgct gttgttccac ctaaatcaat cccaataatc ttcttgtcca ttacaaattt
    24421 cctttctttc ggctttcttc aattcggtgc tctcttttta atatgcgcca cgctgtcatg
    24481 tatgtatcat gatcaatcaa acggctatca tacagattac gcaattcgat agtcattaat
    24541 tcaatgtcat agatgcgtgc acccacatag ataaagatgc caaatttttt gaacagctgc
    24601 tgtacatcat ataagctctc catctttaag cacttccttc atattataca agtaaaccat
    24661 atttttttaa acccagtaaa atacatatta caaaaagaaa tacaaaaact agtgtagaaa
    24721 gaatcctttg gtgtatcgaa tagcttgtct gttgtttcgg tagacccaaa acattcccta
    24781 acactaatcc gccaattagc ccaccgacat gtccccaaac atctacagag gtatccagaa
    24841 gaccaaaaac aaatgtcaac acaataaata aaagaaaatg tctgactagc tgctggatcg
    24901 ctgggttatg tttaaaatga aaccctaaga taaacaacgc accgaacaaa ccgaatatcg
    24961 aagtacttgc accaccagac agaacattcg cttcgttaaa agcaaagctt gctgtatttc
    25021 ccattatccc gctgaacata taaatcaaga aaaaacgcca atggccatag attgcctcga
    25081 tttgctgccc cataaaatac agtatcagtg agttcattac gaaatgcatc agtccaaaat
    25141 ggatgaagat tggtgtcact aaacgccacc attcattgaa gtgaacaaga taaggaccat
    25201 acattccggc ataataaggg atctcaagtc gcggaaataa ttccatcatg aggaagaccg
    25261 ctgtctgtat ccctaaaaaa gcataggtcc ataacggtcg tcttaaccat gcacgcaatt
    25321 ttatttgctg ttgataattc atgaaaacct cccatttcat tttagtcaat aaacaattgt
    25381 tggatcttct gatcaaagct ctccggttgc cattgttcat tcaattgttc acgaaaaaca
    25441 aggctacatg tttttttatg aaaattattt aaataccggt cgtaataacc accgccaaaa
    25501 ccgattcgat aaccggctct attgtagata atacctggta caatgaggag atcgatcatc
    25561 tccggcaaat agagcttctc tgattgtggc tcaagaacac caaaactcga acgtacaaga
    25621 acggactgtt catccatttc ataaaacgcc atctgacgtt tcggcaaagt tttaggtaca
    25681 actattttct tttgttcttt ttctgctcgt tggataaccg gctgtgtatc cagttcaaat
    25741 tcattcgata aagtcgtccc tatcactttt gctgacttcc agtactcgct ggcgaatagc
    25801 tgtttataga ttcgctgttc tttttccttt ttcttgccac ttttggccag atttttcagc
    25861 ttttcgattc ctttccggcg taatcttact ttcaattggt ccaaaaactc acccttcctt
    25921 ctctcataaa atggtatagg aaaaaaagaa aaatagcaat cgaactttca aataaatgca
    25981 taaatagaat aaaaagaaga cctagaaatg attggccttc cttttttaat gaatttcaac
    26041 tgttcagcgc gtgtttttgt ctgataaaaa gtgcatacag aaaatatggc gcaccaaaaa
    26101 gggcaatcaa aattcctgta ggtacttccc cacttggtag aatcactcta gcaaccatat
    26161 ctgcactaag cagaaaaatg gaaccgaaca aaccgctcag aacaaaggtc cagttatttt
    26221 ctttacgtac gactagcttc gcagcatgag gagctagtag tcctacaaaa gataaactac
    26281 ctgtcaccgc tacactaccg cttgccaaag caacagctaa caacaaaagg attttttgac
    26341 ttttagccac gttaaggcct aatccttgcg cgcgttcttg accaaatgat agcaatacaa
    26401 tcctctgtgc ataaagtcct cctagcggaa ttaagataac cgcccatgga agcaatgccc
    26461 aaacataagg ccagctcgca ccccagattg atcccgctaa ccatgcattc acaaatccat
    26521 aattttcctt agataattgg atcgtgcata gcaatgttaa tgcagaaagg ccggcattaa
    26581 cggcaatacc ggacaataag attcgattca tacccaaaaa gccatttcga tcataggccg
    26641 caagatagac acagcaagca gctaaaacac cacctagaaa ggctacaact agcaatagaa
    26701 acggcagcga atagtgcgaa aagaaaccta aataaagcat cactaaaaat cctgctccgg
    26761 cattaatgcc caatatacta gaatctgcca attcatttct tgttactcct tgcaagatat
    26821 aaccagaaac gccaagacaa gctcctgcta ataaactgat cagtaatcga ggaactcgaa
    26881 attctctaac aatcagcgcc aaagaagatg attctttccc tatgaacgtg gcaaaaagct
    26941 gatccaggta aataaaaggc gtaccaatca ttaaactacc tatagacagg atgacaagta
    27001 caaaaagcaa gaaatagaac gtccaatata tttttttcac cgttgttccc tccttacttg
    27061 tatcagtaaa aacggaacgc caatcaatgc aatgactaat ccaaaaggtg tttcaaaagg
    27121 gggattgatc atcctcgctg taaaatccgc caacataacc aatccaccgc caactaaaaa
    27181 agagacaggt agaacataac gatagtccgt tccaaccatt ccgcgaacaa ctggcggaac
    27241 caacagccca ataaatgaaa caggtccgac taacgaaacg gcaatggctg ctaacactgc
    27301 aactagacag aaaacaatca accgcgttcg tttaggatga cttccaagag ctaatgcatg
    27361 tgtttcactc aaatgaatca aagatattgc ttttcctaat gaaaaagaaa ccaaaacagc
    27421 aagtatatat aaaggaaaca atactgctaa ttgtttccat gtgatattgg cagctccgcc
    27481 tacaaaccaa aaagcaagat cctgctgcag attaaagaat tgggtaagtg cttggctgat
    27541 agcagtaaaa aaagaactaa tagctacgcc agctaaaatc agctgtaaag gattcatgcc
    27601 cattttcgaa aactgcaaaa aaccaaatac gagcaaagtt acagcaaaag aacctgccaa
    27661 tgacattaga aacacagaaa aatcagaagc tttaggccaa aaagcaaaac taagggccat
    27721 tgccaacgaa gcacccgcgt tgattcctag taagcctgaa tctgctaaag gatttcttgt
    27781 caccccttgc atcaatgcac cgctcaatgc aaaacaactc cctacaagaa atgctcccgc
    27841 cacacgaggc aaacgaatat ttcgtatgat ttggtgactt tgttttgatc tatcaaaatc
    27901 aaacaacgcc tgccaaaaat ctgctttttc cacagtcgct gcgccatttg aaagagcaac
    27961 aaaaaaaata atcaaaagaa caaaacagac caaaagcagg agtctatttt tcttcatagt
    28021 cctcataatt gatcctcctg ctcttttggc aacaaatcat aggttagaaa aactggttta
    28081 tttgttcttg gaagcgtgac aatcgttgca tcaatctcaa atattttttt catacactct
    28141 tttgtgatca cttcttcagg tgtaccttct gtgatcaact taccgttttt cattgctaat
    28201 aaataatctg aaaaacgtga agcatgatta atgtcatgaa tcgacatcaa gatcgttgtt
    28261 tgtttcattt gattgatttc agtcaataac tgtaggatct ctaattggtg tgccggatct
    28321 aaaaatgttg tcggttcatc caagatcaag atatctgttt cctgagcaag cgccatagcg
    28381 atccagacac gctgggtttg tccaccagac agcgttgcga tatcccgcat acgaaatcca
    28441 gctagacctg tagcatccaa cgcccactca attgcttcat gatcttgcgt gtccatgcca
    28501 ctgaacagtt tttgataagg gtatcgtccg taagatacta cttcttctac tgtcatacct
    28561 gtagggtgtt cactagtttg tgctaataat gcaatttttt ttgcaagttc ttttgaactg
    28621 tatgtagaga tatcagagtt attgacaatg atctttcctg atttcacagg tagtatacgg
    28681 gtaaaacttt tcaataaagt agattttcca cttccatttg gcccaatcaa actagtgatt
    28741 ttcccttcag gaatcgtaac agacatgttt tcaatgattt ttttgttttc gtagccggct
    28801 gcaatgtcag ctgctcttaa tctcgtcatt tcacccatcc tttcgctgaa aatcattctc
    28861 aattatgacg ctgtttcaac atttcgtcaa gcgctacttt catatctata gccaaatgaa
    28921 gcggttgaca gcgttcgaaa tatcaggaat aataaccaat gagaaagatt ctcaattatg
    28981 ttaggaggaa agaaatgaaa ttaaaaacaa aagtattcac attatctagc atattgctgc
    29041 tggctggagt actgagcgct tgctccccct cttcagataa agaaacagct acaactgcca
    29101 attcgacgac ttcatccaat agtactcata cagtcacaga tacacttgga cataaagtag
    29161 agattccaaa cgcgcccaaa cggatcatcg gcagttactt agaagactat ctgattgcac
    29221 taggggaaaa acctatcgcc cagtggactg ttggcagtgg ttccattcaa gattatctgc
    29281 aagatgattt aaaggatgtc ccaacgattt cttatgacct tccttatgag aaagtgttaa
    29341 gctttgagcc tgatttgctg ttgatttctt cttccgctac tgtcgaaggc gggaaatacg
    29401 aacaatatag caaaatcgcg ccaacttatg ttgtaaaaaa cggaacagat gtcacatggg
    29461 aagaacaact aaaagatgtc ggcaaagctt tggcaaaaga aaaagaggca gaaaaaatca
    29521 tttccgatta tcaaaaatcc atcaagaaaa ctcgagaaga actagcagat aaaatcgaga
    29581 acaagacagc tgcagtgttg tgggtgacca acaactcagc ctttatggtc agcaaaaatc
    29641 ggtcaagtgg aagaatcgta tacgacgatc tggaatttgg tgttcctgat ctagtggaag
    29701 aggtctccaa aaaagccact tctgactggt ctgctgtttc ctctgaaaag ctagctgaat
    29761 tagatgctga ctacatcatc cttgtgaaca gtgacaaaga tgctgccatg ttcaatgaac
    29821 caagctggaa gaatctaaaa gcagtgacag acaatcatgt actgcaattc ggacctgaat
    29881 ctagttggct atataacgga cctattgcca gtcaggaaat ggtcaatgat atcaaagaaa
    29941 aactaaattg attttaacta aaaaaagcat cttcacgatt agtcgtgaga tgcttttttt
    30001 cttattaaca aactagacgc atagatttta ttcttctttt gaagcaaccc tattttttac
    30061 tctttcccat ttttgaaaac gatgggcata tagattttct tgatccgtta tcccctcata
    30121 ctcctctata agggcgaagt ctgaaaaatc aatattccca atatacgtat cgccttcaaa
    30181 cgtgctatcg atcaatgttc gccagatccg accagtgtaa ggaagaagtt cttgaaaaat
    30241 ccgactacca cccgaaacat agatatcttc tgagatcgtt tgactatacg acaaaagttc
    30301 atcgatcgaa tgcattactt ccgcattttc tttcgtgcga aaatttggtt gcgttgtcaa
    30361 cacgatgata tggcgataag gaagtgaaag atcccccatt ccttcatagg tctttcttcc
    30421 catcactaaa attcgatcca ttgtatgttc gcgaaagaaa cgcatatcat ttggcaatcg
    30481 ccatggtaac aaaccatttt tgccgatcag accattttta tcttgtgccc acatggaaat
    30541 aaacatatta tccgctcctt atgattttag atgttttgag ataaaaaaag tctcaattct
    30601 ttgaccagca aggaattgag aactttaaaa acattatttt gtttcacggt gcaaagtaac
    30661 tttgcgttcg cgtgggcaat atttttgttt ttccaaacga tcaggattgt tacgtttgtt
    30721 tttgcttgtt agatagttac gttctttaca agaagtacac tctaaagtga tgtttacgcg
    30781 catgtttttc cctcccattt ttacaagaat cctctaagat gactctcaga cttgattatc
    30841 ttaccatgat tttttctaga ttgctagtgt ttttcacgat tttgttaagt aaaactactt
    30901 gacgataaat acaacggttt tacttctcaa acaaaatctt tttctttttg tataaatacc
    30961 cttacacttg tttatataga ttatgttttt taataaaggg ggatactaat atgccatgga
    31021 acatgaagga ctttcctgca tcaatgaaaa acttagataa attgacaaga aaaaaagcaa
    31081 tcgacatcgc aaatgcatta ttagatgaag ggtaccctga taaccgtgcg atcccaattg
    31141 ccattgatca agcaaaaaaa tggtatgaag atgctagtga atcagaaaaa cgaactttcg
    31201 aaaaagaaaa aaatccaagc aagacggatg aacacgatac gaatcctcgt gctggaaaac
    31261 tgcttgattc agatgtgatc gtagaatatg aggaagaaca atggatcgtg aaatctaaag
    31321 gggcaaaaaa ggcaagcaac cattttgata caaaaaaaga agcaatcgaa aaaggaaagc
    31381 aagtagcaca aaacaaagaa tcaactttgt tcatttataa aaaagatggg acaaaagaaa
    31441 aagaaatcga ttattgaaca tatgtaaagg ggttgtgacg aaaatcgtgt cacaaccccc
    31501 ttatgttcga aacaatggtg ttcctctgcc aactccgaac cataagtcat aatatctata
    31561 aaacgggaag agctgttttt tgaaattgtt tcttatcact cagtgttaaa cggtaggaga
    31621 aaacctcttt tttattgata tttgattttc atcaaacagt cacatagtcg attgcttctg
    31681 cttgcggaat cgtttcatct acaaagtaga ctttatacca tgtcaagaaa ctgaagattg
    31741 tccagatttt tctttgctct tctttacgtc cttcgtgatg gtcatctagc aacttcatga
    31801 ttttttcttg atcaaaaaat tcttttgcaa aatctgcttc gaataatgct ttgacttgtt
    31861 cgtagccgtg ttcttctttc aaccaagctt tgattggcac agggaatcct aatttcacac
    31921 gattcgacca ttcttccgga agatgtttgt tcgcagcttg tctgaaaata tcttttgtgt
    31981 tgttttgatt caataaaaac ttacttggta tttgttgagc caatttcatg acttcgatat
    32041 ccaataaagg tactcggact tccaaagaac ttgccataga aagtttatct gcctttaaca
    32101 agatatcttt tggcatccat tgatgaagat cgacatattg catcttattg acttcatctt
    32161 tgattccttc tgttttctcg taatgaacag tcatgatttc atccacagat ggtgctgttt
    32221 gatattttgg tgttaacact tctgatgctt ctgattcttc gaacactcga gcatgaccga
    32281 taaaatattc ttttgcagga gcaagtgatt catataaatg gatccgtcca tggaagtttt
    32341 tcatttttcc gattttacgt cctaaattat agcgtgttcc ttttggtaat tttttcaatc
    32401 cttgagcaat cacacggatg aattttgaat tcgtatggaa tccatacgtt tggtatcccg
    32461 caaacagttc atccgcccct tctccagatt ggactacccg cacactttgt gaagcaagat
    32521 ttgctaaaaa gtacaaaggt acgcaagaag gatttgaatc aggttcatct aagtgatact
    32581 ggatcaaagg gaatgctttg aacgacatgt ctccgtcgat cactgctgct gtattatcta
    32641 agtccaatag ttcggttagt tttctggctt caatcgcttc attataggtt ttatcatcaa
    32701 acccgattga gaaagaatga tctggtttca atacagaggt tacataactg gaatcaaccc
    32761 cgctggataa gaaagaacca acttctacat cactgaccgt atgtgcttca atagaagctt
    32821 gaacggtttc gtcgattttt tcgatccatt cttgtcggga atgcgtttct tttgtttcaa
    32881 aatctgcatc ccaatattgc tgaatgtcta tttttccatc tttatacgta taataatggc
    32941 cttcaggcaa acgatagaca tctttgaaga atgtttcgcc gtttaatgga gaatattgga
    33001 aagtcatata aggtttcaac gcttctttgt tcaattcttt gttaaaatca ggatgaggca
    33061 gaaaactttt gatttctgaa ccaaacataa atgttccgtt catttctgca taataatatg
    33121 gtttgatccc aaagtggtct cttgcaccga ataactcatt cttttcatta tcccaaatag
    33181 caaaagcaaa cattccgcgg atcttttgca ataattctgt tccccattct tcgtaaccat
    33241 gcaaaaggac ttctgtatct gcatgtgttt gaaaaacgtg gccagctgca atcaattctt
    33301 ctctcaaagg ctggtaatta tagatttcgc cattaaaaat aatgatcttt gtccgatctt
    33361 cattgtaaat aggctgagta ccgccctcta agtcaataat acttaatctt ctgaatccta
    33421 aagcaacatg tttatcgata tattctcctg aactattagg tccacggtga acgattcgat
    33481 ccatcattgt attaataatt gtttttttgt tgtccttgtc gttaacaaaa cctacaatac
    33541 cgcacattta actttcacat cctaatataa aattactgtt tatttcattc atatgcatct
    33601 tatcatagaa taaatagcgg acgaatattt tctctcttac aatttcgtca cgtgaaaaca
    33661 aaagatgaaa gtttctgttt ttacagaaaa taaagtccca atatctaatt taccatgttt
    33721 tcttttttga ggctacctta ttctcttcgt gttaaaaaaa tattagtgga tgctatttcc
    33781 aaaaaaacca gcaaaaaaag acacgaccta aagatcgtgt
//
LOCUS       DACDXO010000025        33736 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E23605_2017
            SAMN12639989-rid9881463.denovo.025, whole genome shotgun sequence.
ACCESSION   DACDXO010000025 DACDXO010000000
VERSION     DACDXO010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639989
            Sequence Read Archive: SRR10023389
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 33736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741914.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:42:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,972
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 33 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 17 of 73
            Pseudo Genes (multiple problems)  :: 10 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33736
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639989-rid9881463.denovo.025"
                     /isolate="E23605_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            214..498
                     /locus_tag="IWM35_001378"
     CDS             214..498
                     /locus_tag="IWM35_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HAP9919238.1"
                     /translation="MREIYSFESKIYASEVDKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNMPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(779..1198)
                     /locus_tag="IWM35_001379"
     CDS             complement(779..1198)
                     /locus_tag="IWM35_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HAP9919239.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIKEPVYYKDGEKTL"
     gene            complement(1200..1784)
                     /locus_tag="IWM35_001380"
     CDS             complement(1200..1784)
                     /locus_tag="IWM35_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HAP9919240.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFGIVNEHSKRWQGD"
     gene            complement(1831..3306)
                     /locus_tag="IWM35_001381"
     CDS             complement(1831..3306)
                     /locus_tag="IWM35_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HAP9919241.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPAMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGMSAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            3487..3798
                     /locus_tag="IWM35_001382"
     CDS             3487..3798
                     /locus_tag="IWM35_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9919242.1"
                     /translation="MKKMKESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            complement(3913..6249)
                     /locus_tag="IWM35_001383"
     CDS             complement(3913..6249)
                     /locus_tag="IWM35_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP9919243.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTDHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            6391..7059
                     /locus_tag="IWM35_001384"
     CDS             6391..7059
                     /locus_tag="IWM35_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9919244.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERAGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            7062..8105
                     /locus_tag="IWM35_001385"
     CDS             7062..8105
                     /locus_tag="IWM35_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP9919245.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFLQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIG
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(8342..9223)
                     /locus_tag="IWM35_001386"
     CDS             complement(8342..9223)
                     /locus_tag="IWM35_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP9919246.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARNFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            9382..10110
                     /locus_tag="IWM35_001387"
     CDS             9382..10110
                     /locus_tag="IWM35_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HAP9919247.1"
                     /translation="MPTGVLINVGSVLLGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            10266..10583
                     /locus_tag="IWM35_001388"
     CDS             10266..10583
                     /locus_tag="IWM35_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAP9919248.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            10576..11184
                     /locus_tag="IWM35_001389"
     CDS             10576..11184
                     /locus_tag="IWM35_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HAP9919249.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            11200..12030
                     /locus_tag="IWM35_001390"
     CDS             11200..12030
                     /locus_tag="IWM35_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HAP9919250.1"
                     /translation="MRLKHFLITGIGLMLTGGLLAGATYTLGAQKSLTWDNGPRLIDM
                     VTETKKIKDANKIVIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKK
                     STGAIISLEDTSAQITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSF
                     NEFTGGTITAKDELGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSL
                     HEMENSGYTIQTSGESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD
                     "
     gene            12168..12680
                     /locus_tag="IWM35_001391"
     CDS             12168..12680
                     /locus_tag="IWM35_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HAP9919251.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQARGWYITEDKELELEGVMARIRK
                     KIKKVTLDIY"
     gene            12706..13578
                     /locus_tag="IWM35_001392"
     CDS             12706..13578
                     /locus_tag="IWM35_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HAP9919252.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            13754..14116
                     /locus_tag="IWM35_001393"
     CDS             13754..14116
                     /locus_tag="IWM35_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAP9919253.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            14125..14877
                     /locus_tag="IWM35_001394"
     CDS             14125..14877
                     /locus_tag="IWM35_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9919254.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            14891..16693
                     /locus_tag="IWM35_001395"
     CDS             14891..16693
                     /locus_tag="IWM35_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9919255.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIILGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            16970..18010
                     /locus_tag="IWM35_001396"
     CDS             16970..18010
                     /locus_tag="IWM35_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAP9919256.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMGNGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSPDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQVR
                     EFVTEYKINIKK"
     gene            complement(18062..20206)
                     /locus_tag="IWM35_001397"
     CDS             complement(18062..20206)
                     /locus_tag="IWM35_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP9919257.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLSLDKSFWLDLPLKK"
     gene            complement(20563..21936)
                     /locus_tag="IWM35_001398"
     CDS             complement(20563..21936)
                     /locus_tag="IWM35_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9919258.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            complement(21936..22331)
                     /gene="rbsD"
                     /locus_tag="IWM35_001399"
     CDS             complement(21936..22331)
                     /gene="rbsD"
                     /locus_tag="IWM35_001399"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002829567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HAP9919259.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            complement(22359..23276)
                     /gene="rbsK"
                     /locus_tag="IWM35_001400"
     CDS             complement(22359..23276)
                     /gene="rbsK"
                     /locus_tag="IWM35_001400"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HAP9919260.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            complement(23384..24397)
                     /locus_tag="IWM35_001401"
     CDS             complement(23384..24397)
                     /locus_tag="IWM35_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP9919261.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            complement(24478..25077)
                     /locus_tag="IWM35_001402"
     CDS             complement(24478..25077)
                     /locus_tag="IWM35_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP9919262.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTIKKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            25263..25964
                     /locus_tag="IWM35_001403"
     CDS             25263..25964
                     /locus_tag="IWM35_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9919263.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            25974..28685
                     /locus_tag="IWM35_001404"
     CDS             25974..28685
                     /locus_tag="IWM35_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HAP9919264.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLPQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGPIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            29082..30710
                     /gene="treC"
                     /locus_tag="IWM35_001405"
     CDS             29082..30710
                     /gene="treC"
                     /locus_tag="IWM35_001405"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HAP9919265.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGENMSEGNGWNALFFNNHDQPRALNRFVVIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(30770..31951)
                     /locus_tag="IWM35_001406"
     CDS             complement(30770..31951)
                     /locus_tag="IWM35_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP9919266.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            32203..33726
                     /locus_tag="IWM35_001407"
     CDS             32203..33726
                     /locus_tag="IWM35_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HAP9919267.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKDGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAGNESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
BASE COUNT        10690 a         6051 c         6863 g        10132 t
ORIGIN      
        1 aggaagaggt tgtgagcctg ccgtttaact ctgagtcaca aggagcaatt tcaaaaaaca
       61 gcttctcatg tttgttgact attgtgactt gtgaccgagg agttggcagg agaacaccgt
      121 ttattcgaaa aaaggaactg cgacaagact tttgtcacag ttccttcttt tttttgctat
      181 gctatactag atgtaaaaaa taggagaaat aaaatgaggg aaatttattc ttttgaatca
      241 aaaatctatg cttccgaagt cgacaagggc ggggcatata ttatttttcc gtatgatata
      301 agaaaagaat tcggtgctgg aagagttaaa gtccaagcaa cttttgacaa tatgccgtat
      361 gaaggcagta tcgtgaatat gggcgtgaaa aatccagatg gttcagtctg ctacatcctt
      421 ggactacgaa aagatattcg taaaaacatc ggaaaagaaa tcggtgatat tgtagcagtt
      481 acagtgaagc aaaaataaaa gaggttgtga caaaagacgt gtcacaacct cttgataaac
      541 ggtgttcaaa agccagtttc tcggtagtaa gtcaaattta tgcaaaaatg tggaaaccca
      601 tgtttacaaa atttttctta ctactcgaaa cgaagcggct ttatcacaac ctcttgataa
      661 acggcgttca atcagctgtc cctcggtatt agctcaaaaa cggcaaaata tgagaagcta
      721 tttttccatt tttttctctc atactcaggt cataacgctg atttcacaac ctcttctttc
      781 ataatgtttt ttcaccatct ttataataaa ctggttcttt gatatggaaa tctatttttt
      841 ccaaccaatc taaaatcttg taacctaact cttctgacat cggaaaagag gcgttgattt
      901 gtgcattgga gatatggtca acatggatac cggaagtgat cgtacaactt cccggaacgg
      961 cagttttgat gatcacagct attttttcgg ctaagacatc gtctttatgg aaccgaccat
     1021 cgtggctagg gaaacgtaca gtttgcatct ctgtatcacg agaaaatgta gtgactgttc
     1081 cgatatgtgg tgtatctccg cctgttaaag tgatcaataa gtctttaccg attactttaa
     1141 cagcagcttg gatcgtgaaa cctagtttgg ttacttcaac tatcgtttct ttttccatat
     1201 taatcaccct gccaacgttt tgaatgctca tttacaattc caaattgatc caaaagtttc
     1261 attgtatggt gattaacaag atcttggatc gtttgaggct gattataaaa agcaggaatt
     1321 ggtggtatga tttgcacacc tagtcgtgct aatttagtca agttttccaa gtgaatggca
     1381 tttaatggcg tttcacgagg aacaatgact aactttcgtt gctccttcat tgttacatca
     1441 gcagcacgtg caatcaggtt gtcgcctaaa ccaacagaga tacctgcgac tgttttcata
     1501 cttgccggta cgatgatcat tccatcggtc aaaaatgatc cgctggcgac agcagcacct
     1561 aaatctttgt ttgaataatg gtaatcaaac aggctttcga attcagttag tgataagtcg
     1621 gattccagct ttaagttctg ttttgcccat ggactgatga ttccgtgtgt ttctatcatc
     1681 ggatattctt ttagtttctt cactaggttg atggcataaa tggtgcctga agcgccactt
     1741 acaccaatca cgattttttt cttccgttgt gccaattgtt ccattttccg cactcctttt
     1801 tatgatgtca aaacagttgc agatcattca ttatttcaaa tatttttccc aatcttctac
     1861 ttcttggaat tttgcacgta caaaatgttc tttcatatcg aaaggaactg taccgtcaaa
     1921 gatagttttt gcactcatcc cacggaaacg aatagagcca ggattatatt ctggtaattc
     1981 tgatgggtcc aatggatggt tcctcattcc tgggatcacg atgatatctt tatctccttg
     2041 gaaacgagtg ttgaaagtcc aaactacatc gttcatgtcg aaaatatcaa catcttcatc
     2101 gactaaaatg actgtcttca attctttgaa ggctgataaa gcaagaatag ctgcttgacg
     2161 ctgaatacct tcatccgctt cgttttcttt acggatttgc atgatggtca tcaatttacc
     2221 accacctgca ggaggattgt agacatttac tacttttcct ggaatagctt tatcgaccaa
     2281 ggcaagaata cttgcttctg taggaatacc ggccatagat acgtgttctt cactgggacc
     2341 aatcgttgtt tgcatgattg gattgtcttt tcggtgagta actgctttga ctttcactac
     2401 ttgtacagct ggattagcat ccccagtata gccaggaaat tctggcattg cttttcctga
     2461 gttcgtgttg atatcttctt gcattgtttc atttggcatg atttctgctt caatgatgaa
     2521 ttcagcacga gcaataccca cttcatcgac tgctatacca tcaacgattt gaataggttc
     2581 gttacgcaaa gctcctgcac accatagttc attgtagcca agtggagtgg taggtggttc
     2641 gaaagtcgtg ccaatggcaa ttgctggatc taaaccaata ttgatagtga taggcattgg
     2701 tttattcaag gcttcgaatt gtttttggaa gtgaccaatg tggcgtccgc caggcatgat
     2761 gtacataccg atcgtatctt tatcttctag aaccatacga tggatcgtta catcgctcat
     2821 tgttttttct ggatctgatc ctaagactac acccattgtg atgtatggac cagcatcttc
     2881 aggagtattt gtaggtgcag gcaaaatgtt gcgaatatcg aagtctgcat cggtagctag
     2941 atgaacgact tcttgtgctg gagcgtctgc tttgtctact ttcacaggtt tgattggatt
     3001 ttctacagcg tctttcaaga aacgaccaag tgttcgataa tcatggtgca aaatttcacc
     3061 tacacgttta cgactagcca ttgttccgat gttcacacgt gtgccgggga aacctttgat
     3121 attgttgaaa gtcatagctg gtccttcttg agtcggacgt tttactgtac cgccagcacc
     3181 gatataacgg taaacaccag ataattctgc ttcaggatcg atttcaatat ctgtttcgtg
     3241 atattgacct ggaagtgttt ttagttcttc gatgactttt cttaaatcat acggttgatc
     3301 tgtcatgaga gattcctcct tagttgtctt tacctatcta tagtaaaagg tgaaaaagag
     3361 gaaaacaatt caacttgttc ggtaaagtat tctacaatgg aatggatgcg tttttttgat
     3421 aatagaataa actatgatat acttatcact aaaagaaagc gcatacttag tgttggagga
     3481 aggaaagtga aaaaaatgaa ggagtcattt cctaaagcag taaaagtgga aaatatagcg
     3541 aatattttga aagttacatt tgaaaacgga gaagtaaaat acgtgaaatc tcattggaca
     3601 gaagagataa ccgatgcctt gcaatttgga aaaaaaggaa gaggaaaaag aaaaaatctt
     3661 ctcgcacttt ctcgtaatat gtggattggg actgaagtaa cgattgaagc agatggaaca
     3721 gtattcataa atggaaaaga caggtatacg ccagaagagt tgtggtacaa aggaaagaaa
     3781 agtataccag aattataaaa aataattaaa aaaaacagcg cgaaacaagg aacatattac
     3841 ttcctcgttt cttgctgttt tctactattt cagcgctctt atgactcaaa tctaaatgtg
     3901 gttgtcacaa ccttattctg cacgcaaagc gatagcggcg tcttttttcg ctgccattcg
     3961 tgcaggaaga tgaccgccaa gcatcgtcaa tatagtagag acgatcacta gaataatcgc
     4021 atggacagga tttagttgag caacattttt tagatccgtc atgttgtata agatgctatt
     4081 gatagggaac gtagctagcc atgcaataaa cacacctaat atcccagaag aaatacctaa
     4141 aatacacgtt tctgcgtcaa atacgcgagt gatgtctttt ttacgtgcac ctaaagcttt
     4201 tagtacaccg atttcttttg tccgttcaat cacagatgta taagtaatga tactgatcat
     4261 gatcatactt gttactaaag agatccctgc aaaagcaata aggacataag tgatagcatc
     4321 catcaatccg ccagtcagtt cagtcatcgt tccagctaaa tcagtataaa tgattttatc
     4381 ttcatctgat ttgcctttat tgtaatcatc tagataatcc aagatctttt ctttgtcttc
     4441 aaaattgttc ggataaatca tgatacttga aggcaagctg tctccaccta aataactaag
     4501 caatgtttgt ttggcatttt cgtcaacttt ttcagtagtt aagacattta caccactgtc
     4561 tctttgagct ttgacgattt ctgattcttt gttttcgttg acgatttgag tcgttaattg
     4621 atcgctataa gcgattcctg gagataagag attcattgta gaagaagatt tcacgcgtag
     4681 gatccccgag atcttcaatt catcagaagc attctgatac atcgcatcat aatctgtatt
     4741 agggataaaa ttcccagttg gaagttttgt atagtaagta ttgttgttta ctaatttgaa
     4801 tgttgtacca acgatatcat caaaatcaag tttttcgtct tctttcacat caaatccaag
     4861 attcttcaat gcgttgatat tagtattatt attgccgtct acgatcagca cgacatctgt
     4921 tgctgaggct ggataacttc cggcaagtaa agaataattg tcttttaaaa agttttcttt
     4981 gctagtgtca agctgtgtag ggaaacttga cactcccaca cctgtcattg ctgacatcgt
     5041 actagataaa gaaagagatt ccgtatcagg attttgattc gagaagctga caggctgtac
     5101 tttgccattt acatcacgta aaagattgat tcctgtagta cgggtaaatc cgatattatt
     5161 acttaagtta ggatcgatgt ctgttacgta atcgacgtat gtttgatcaa tcttattcgt
     5221 gtgctgtgct ttatcttctt cgctgacttt tgcagtaatc gttttgctgt ctggataatc
     5281 cgctttgctt gcacccaatc cggcatcgtc acgagtctgg ttagtcgtta ccttagaaat
     5341 agtaatcgga aatttcgcca tcgtttcagc ttgcgtgtta tcgatttgtt tttggaatcc
     5401 agaagaaagg gacagaacga tcgcgattcc gatgatccca atactggaag caaaggaggt
     5461 caagaaagta cgtccttttt ttgttcggat attgttgaag gaaagcttta acgctgtcca
     5521 aaaactcatc tttgttctgc gtaagttaaa ttgatcatct tttggtcgtt cgatgtgagg
     5581 atgggaatct gttaagatct taccatcaga aaattcaatg atccgatctg cgtattgatg
     5641 ggccagttca ggattatgtg tgaccatgat cactaatttt tctttcgata actcttggat
     5701 caattccatg atctggatac ttgtttctgt atctaacgct ccggttggtt catcgcacaa
     5761 aaggatatct gggtcattgg ctagtgctcg agcgatggcc actcgctgca tttgtccacc
     5821 agataactga tttggctttt tatgcatatg atctgtcaag ccgactcggt gcaatgcatc
     5881 ttctgcccgt tttcgttttt cgtctttaga aacaccactt aatgtcatcc caagttcaac
     5941 gttttcaata atgcctagat gaccaatcaa gttgtaactt tgaaaaatga aaccgataga
     6001 gttgtttcgg taggcatccc agtcactgtc cttaaagtct ttagtagact ttccgttgat
     6061 taccatatca ccggaatcat aattatctag tccaccaatc acattcagca tagtcgtttt
     6121 accagaacca ctaggaccta aaatcgctac gaattctttt tgtctgaaag cgacagacac
     6181 accatccaat gctttggtgg tggtttcgcc gactttataa tattttttta tatcttttaa
     6241 ttgaagcata atcacccttc tcccgtattg aaatattcgg tatactaata agtatatcag
     6301 aacaatgatc gtagtatacg aaataaatgt taactgaaga tgaactacaa gaaaaaatct
     6361 agaaattttt caaaaaacag agggagatca atgaatcgta ttttagtagt agaagatgat
     6421 ccgcaattac agttgcttta ccgttctgtg ttggaacgag cgggcttttc agtcatcacc
     6481 gcattgaatg gagaggatgc gctgaaaaaa ctagaagcga cacgtataga attaatcatt
     6541 acagatatca tgatgccgaa aatggatggc tatgacttgc tagactcatt acgtagcacc
     6601 agatttgaca cacctgttct aatgattacg gcaaaggccg attttgagga taagaaacgc
     6661 gggttcaaat taggagcaga tgattatatg acaaaaccca tcgatgtcaa tgagatgatc
     6721 ctacgtgtgg aagcattgct tcgacgttca aagatcaatt acagcagtag cttgataatc
     6781 ggacaaacaa aattagagct agatgcttat acagtgttac aagatcagca ttcgttgatt
     6841 ctgcctcaaa aagaatttct gcttctgtat aaattactgt cttatcccaa caagatcttc
     6901 actcgccaac agttgatgga tgatatttgg ggattagata cgaatacaga agaacgaacg
     6961 atcgatgtac atatcaaacg tttaaggacc cgttttgaaa agacggaaga ttttcaaatc
     7021 atcacagtac ggggactagg ctataaggcg gttatttaaa gatgaaaaaa aagctatttt
     7081 cacaattatg gatctatttt gctagtattg tcttcatttc gatcttagtg acactgctgt
     7141 gttttctcgg ttttattttc ttattgtctc gacattctat ctcgcctgct gaatcggctg
     7201 gaaaaccaac gttatacccg ttattcgtct ttgcggggtt cagtatggtc gtgggtacag
     7261 gaatatcgat ttttgttggg cggcgtatcc tgcatccaat cagtgcgcta gggacaaata
     7321 tgagtctggt agcaacggga gatttttcga ttcgaatgga tgaacagcag aaagtagcag
     7381 aagtacagca actgtataaa gatttcaacg ttatggtcca agaactcaat agtatcgaga
     7441 cactgaggaa tgactttgtg tccagtgtat cccatgaatt caaaacacca ctagcaacaa
     7501 tccaaggata tgttcaattg ttacaagccc caaatctctc tgacgaagaa cgccaaatat
     7561 ttttatatcg gattatcgaa agtatcacgc agctttcaca attaacagag aacatcttaa
     7621 aattgaacaa actggaaaac cagcgtatcc aactggaaaa gaaagagtat cgattagatg
     7681 aacaaatcag agaagtaatc gtttttttgc agcctaaatg ggagaaagaa cagctagaat
     7741 tggatatcga acttgctgca gtaaattaca caggcaatga agagtttttg taccaagtgt
     7801 ggctgaatat catggataac gccataaagt acaatcaaat caatggacag attcatatca
     7861 aactatttga gacagccaca gagattggac tggaagtgac cgattcaggt gtaggaatga
     7921 atgaagagac cagagatcga atgtttgaaa aattttatca aggagataca agtcgtcaaa
     7981 tttctgggaa tgggctagga ctttcactgg ttaaaaaaat tctagaactg catgatggac
     8041 gtattgatta tagcagtatc gaaggagtag gaacgacagc aatgatacgg ttaagtaaac
     8101 aataggaaaa agaataaaac ccctaaaaat tctattgaag gtttttaggg gtttttagtc
     8161 gtttttttgt gattagggaa atgttcttac ggttcttttg gcataaaaat cgctgctttc
     8221 ttattgaact acgagaaatg ttagaggtgt cattgaaaca aggacatgat gcaagaaaga
     8281 gagcgaaagt catgcgtcgc tctctttttt tgggtggata gatggacaga aaatgtaggg
     8341 cttagttttc ttcttgattt aaatactccc aaaatgtatc tgaagccaaa gaatgctcac
     8401 cgactttacg agtaaatgca agataactct ctgttgttgg agaaataggg agaatcacag
     8461 tattttcttt taatagattc ttgactgttt tttccataac aatagcaatc cccatgtcat
     8521 ttgcaatcat attgatgatc aagtcgattc ttgctccttt ataagagatt tttggttcaa
     8581 atccagcttc gcgacacaga tctagtacag gttgatacaa gttcgtagtg gggccaagga
     8641 gcaaaaagtt ctcctttttc agttcgctta gatccaattt ctttttacta gctaaaggat
     8701 gatttttagg tagaactgct acaaaatcgt ctgtttcagt aggcaaccac tcaatcgaac
     8761 ttggtggggc ctggaaattt ctagcaaagt ggatcgtgtc actatgttca ttggcgtcat
     8821 tgtaagttaa ttcgacactt tccacttctt tcaactggac tgtaaattca ggatggcgtt
     8881 tcaaaaactc ggtgattttg gaaaaacctt tataattgat cattgtcggt atcgttaaga
     8941 tatttaatga aagattgttg tctttcgtat aggtttcaag cacttcttgc atagcatggt
     9001 attgttctac gacatttttt acatatggga gcaataagct tccagcttcg gtcaattcga
     9061 tttttctatg ggcgcgttcg aataatttga cgtctaattc tgtttctaac gaaagaatct
     9121 gtttagaaat atttccttgg gtggtgaatt gtcgatcagc agttttggtg aaattcattg
     9181 tttcggctaa atcaagaaaa acttcgaatt tatggatatc catcatattc ctcctactta
     9241 ttccaataaa gaatacttta acacaaaaaa tgaattggtg taatcttctc tgtctgttac
     9301 aatctttctt gcagcgaaag caataattac agaaattttt ctgatttttt tcgcttcgta
     9361 gaaaaagaga ggagtttttt gatgccaact ggagttttga tcaatgtagg ttccgttttg
     9421 cttggcggac taattggcgg attagtagga aataaattaa gtgaacattt taaagcacag
     9481 ctgacgatgg tttttggtgt gtgttcgatg gggatgggaa tctattccat tgcgccgatg
     9541 aaaaatatgc cggctgttat ttttgcttta gtgataggaa cagcgattgg tctgattgtt
     9601 catttaggta atgggataaa taagggggct gcgctgatgc aagtacctat ttctaaaatc
     9661 ttccctagtg aaaagcttgg tatgacccac gatgaattta tcagcacatt agtgacggtc
     9721 attgttctat tttgtgcaag tggtacgggg atctatggaa gtttagattc tgggatgaca
     9781 ggagacagta cgattttgat ttctaaatct gtccttgatt ttttcacagc agctattttt
     9841 gcgtgtaatt tagggtatgt cgtttctgtg atagcaattc cccaattcat tattttttat
     9901 attttgttct tattggcaaa atttatttat ccattgacta caccagacat gatcttagat
     9961 tttaaagctt gcggcggatt cttgatggtc gctaccggtt ttagaatgat caacgtgaaa
    10021 atgttcccag tagctgacat gatacctgca atgatcgtta tcatgccact gagctggatg
    10081 tggacaaatt ggattatgcc actgttataa taaaaatcaa agagaagcca ttttttgctg
    10141 actaaaaagg atcaaatctt ttagtcagtt tttttgtatt tgacaaactg attttgaaac
    10201 tatattatat gttgtataat atagattgaa ataaaatatt acacttgaga atagaaggtg
    10261 acgtaatgca catccagatt ccaactgttc ttcttgatgg tactgtgcta gcagcattaa
    10321 gcaaggaaga tatgtatggg tatgccttaa caaaaatcgt acaagaaagt ctctctgtca
    10381 gtgaatctac actatatccg gttttgagaa gattgaaaaa agaaggatat cttgagacct
    10441 atgatgaacc ttttgaaggc agaaaccgtc gatattactc gttgacacca atgggaagtc
    10501 gtcatttaga agaaattatt gatgaatgga ttgtttttcg agattcttta gaccggttat
    10561 tggaggagag cacaaatgaa tgaatatttg aatgaagttg tggatcatct tcaagaaatt
    10621 cctgaagacg atcgttttga tttgatccag tattatgagg aatatttttt agattctgga
    10681 aaaacattag atcagatcat tgatgaatat ggtacaccaa agcagtttgc cttaaaatta
    10741 aaaatcagtt acttttcaga agaagaacaa gcagaaaacg agttagtgaa aaatccaaaa
    10801 agacaaattc gcctgatttg gttaatcatt atcggtttat ttgcttctcc tgtcctcatt
    10861 ccactagcac tggggtttat attggttatt gggggactat tggttgcttt actagcgctc
    10921 atttttagta tctatgtttt atgtatcagt atcttaggtg ttggcctatt catgatcatc
    10981 tcaggaatca gtgtgtttgg acaatcgatt ctgagtggat tattctttat cggtgtcggt
    11041 ctgttcgcca caggaagcgc agtatttatt tcgccactgt tgctgaaagg aacaaaaggg
    11101 cttttttatg tcatgatgaa ctttgttaaa tggatgggac gacgttttat tacaaaaaga
    11161 gatctgcatt cagcgggcgt ttgaaggagg acacataaat tgagattgaa acattttttg
    11221 attaccggta ttggtctgat gcttactggt ggcctcctag caggtgcaac ttatacatta
    11281 ggcgctcaaa agagtctgac ttgggataat ggtcctcggt tgatcgatat ggttactgag
    11341 acaaaaaaaa tcaaggatgc aaacaaaatc gtcatccatg cagaaaatca aacagtttcg
    11401 gtcatacgcg gattggattt tgagataaaa acgacttatg ataagacaga aaagccagaa
    11461 ataaaggaga aaaatggcgt gttgactgtg aatgcagtca aaaaatcaac aggtgcaatc
    11521 attagtttag aagacacaag tgcacaaatc actattgttg tccctgcaga tcatgaatta
    11581 tctgaattga caatcgaagg gaataatagt gcaattgata tcaacgaagt atcaatcaaa
    11641 aaagtgaata ctaagacaca tgatggctgg gtatcgttca atgaatttac aggaggaaca
    11701 ataactgcga aagatgaatt aggctcactt cgattagatg aggccaatgt ggataacttg
    11761 atcgtcacag gtgaaaacag caacttccat ctagaagaca gtttgatcag caaagaagga
    11821 aaagtcatcc taaacggtgg caatttatct ttgcatgaga tggaaaacag tggctatact
    11881 atccaaacgt ctggtgaatc ttatattgaa aaagactacc agccaataac aaatactttg
    11941 ttagaacaag gacagaaaaa gctaaagatc actgctaaag acacatcgat caatattacg
    12001 acggatcaag aggacaatga aacagattga tcctgtactc aactggatat tttaagaaaa
    12061 aacagcaaag atccttccaa tctttgctgt ttttcatcgc ttaacgcaca taactatagt
    12121 aaaataagaa agaaaatatc taaaaagaaa acagggtgaa agagtatatg aaggtagaag
    12181 aaggtcaaag agaaaagtta aaaacagaaa tggaaagatt acatacttat atcactcaac
    12241 taagccaaac cttctatgat ccagacaaag aaaaagtgat ggttaattac ccgaacaatt
    12301 cagaaggtcg ccaattagag caagtgtacc atgaagtttt caaacattta ttgacggtga
    12361 agaaagagct ggattattat tctttaccga ttatcgatac aggaatattg aagtatgatg
    12421 ggaagaagga acgatttatc tttaaatctg ttcgggaaga tttgcctctt tcagcaggga
    12481 tggatctaga ggtcttggta gaagactatt ttacagaaga aaagcactgg gttcgaacaa
    12541 aattggatta tttaccgcag gcagccggag ggacacaggc aaggggttgg tacatcactg
    12601 aagacaaaga gttagaacta gaaggtgtaa tggcgcgtat tcgaaaaaaa attaaaaaag
    12661 taacattaga tatttattga aggataaagg agcgcaggaa gtagcatgag agaaaaaaga
    12721 gcattttata tcaatggtta tgcagggatt gtcggattgg tcgtattagc tttagttgga
    12781 ttgtttttct tctatttagg tatgtggcaa gcgaaagttt gggcactttt tttaagtatt
    12841 tttctttggt tgatcacgtt attgttgtta agttcggcaa ctgtagtcag tcctaatcaa
    12901 gcaaaagtaa ttttgttttt tggacaatat ttaggtacga ttcgagaaaa tggctttttc
    12961 cttacgatac ctttggcaca aaaaatgact gtttcgttga aagttcggaa tttcaatagt
    13021 tctgttttaa aagtcaatga tttggatgga aaccccatcg aaatctcagc agtagtagtc
    13081 tttaaagtcg ttgatacagc aaaggcatta ttcgatgtag cgtattatca agattttgta
    13141 gaaatccaaa gtgagacagc cattcggcat atcgctagcc aatacccgta tgatacattc
    13201 aatgagaatg atttgacgct tcgaggaaac acgacggcag tgtctgacga gttgcaaaaa
    13261 gagctgcaag agcgtttagc agtagcaggt gtcgaagtaa tcgaaacacg cttgaaccat
    13321 ttagcctatg caacagaaat cgctagtgcc atgctccaac gtcaacaagc aaaagccatt
    13381 ttgtctgctc gacaaaccat tgttgaagga gctgtcacaa tcactcaaat ggccttagag
    13441 caaatcgaag atggacaaga aatcaatttc accgatgatc ggaaagtcca attgatcaat
    13501 aatttacttg tctcaatcat tacagacaaa ggaacgcaac ctgttatcaa tacaggcgac
    13561 atgaaagacc gtaagtgaca tactttttgt aagataggaa gaaggagttt aggacggatt
    13621 attgtcttga actctttttt tagttgtttt aaagaaaatg acaaaattga aagaagaatg
    13681 atagttgatt cgaaggcaga attgttcgct tttttctata atcagtatat aaagtaagag
    13741 agaaggggaa aaagtgaaaa aacttctgat cggtctactt gttttcatcg ttgtggtact
    13801 tgcaggggtg aaaatcgcag attacgtagt tatgggtgga gaagaatact atgtacaaat
    13861 tacgacagat ggtacaaaaa cagtttcaaa atcagactct ggacaagaaa cagtcaacta
    13921 tagatatatc ctgcctggct atacgaaaga gggtaaggaa aaagaactgg acttcaaggg
    13981 acaattgact cgaccgcttc gtaaaggagc atatttaaaa atcacctgga acaaaaataa
    14041 aggtgtcact tcgtatgaag aagtcgaaaa aaaagacgtg ccgaaaacag cactggaaaa
    14101 actaattaaa gggtagggaa agaaatgaac aaaatagtcg aagtacagaa tgtaacaaaa
    14161 gtatacggaa aaaacaatga gaaaaagacg caagcattaa gcggcatcag cttcgatgta
    14221 gaaaaaggcg aatttattgg gatcatgggc gcttctggtt ctggtaagtc gacacttttg
    14281 aatattttat ccacattaga taaaccaaca gatgggcata tccgaatcaa tcaaaaagat
    14341 gtgaccacat taaaaggaaa tcaactagca gattttcgtg cgaatgagat tggctttatt
    14401 ttccaagatt tcaatttatt agaaagcttg acagcacaag aaaatatcgc tgttcctctt
    14461 tctttacaag gcgtcagacc aaaagaaatc aagcaacgtg tccaaaaaat tgcagaacgt
    14521 ttatccatct ctcatatttt ggaaagctat ccttctgaga tttccggtgg acaaaaacaa
    14581 cgggtcgccg ccgcacgtgc attgatcaca caaccaacga ttctattagg ggatgagcct
    14641 acaggtgcgc tggattccaa aagtgcccgg gatcttttag atacgatgga cgaattgaat
    14701 acaaaagatc atgtatcgat tcttcttgtg acgcatgatc cgttttctgc aagttattgc
    14761 caacgtattt tattcatcaa ggatggcggt atccatcaag aagtgaaacg tggagatcaa
    14821 agccgtgatt ccttctatcg agaaatacta tctattcttg gaaacttgga acagtagggg
    14881 gagattgact atgctttgga aattatcact tactgggatc aagagccgtt taagagacta
    14941 cttggtgtta ttctcaggat tagttatggc atctgctatt ttttacatgt ttgaatcaat
    15001 ggcaagcaac gaagcttttt tgaaaagtaa tacgatcgtt gcggctacag ttatcatttt
    15061 ccgattcggt tctgttttac tagggatcat cacattcgtt tatattttat atgcaaattc
    15121 ctttttgatg acgatgcggc aaaaagacta tgcaatgttt atgatgctgg gagcaaaagg
    15181 cagaaaaatc gcacagatga ttttttctga aacattctta gtagggatcg cagctacttt
    15241 agtgggttca gctgtcggtg taggattgac aagtatcgtc caccgattat tagtggatca
    15301 gttagatctc aatatttcgc attttacacc ttttagtatt cacgggttgt tgatcactgt
    15361 tttgtttttt gctgttttgt ttttattagc tgctgttgtc aatgcttttt cgattgtcaa
    15421 aaaaccgatt ttagcattga ttcgtgcgga tcagacacca acaagaatca agcagcataa
    15481 attcttgttt ttccttgaag tagtactggg aattgctttt ctaggctgcg gttactatat
    15541 gatgaaacac gtggggacct atcaagtctt tggtataggg gtagccttag ttacgatcat
    15601 attaggaact tatttcattt tccattctgt gatcattttc tttttatcat tactaaagaa
    15661 atcagaacag atttcattga aaaaattgaa caatttcaca ttgtcacagt taaactttcg
    15721 tattcgtgaa tatacacaaa tgctctcaat ggtcgctatg ctgtttgcct tggcactggg
    15781 cgctttgacg gtaggattag gttttaaaaa tgaaattgca gacttgacag aaaacaattc
    15841 tggatatgat ctagtgatga ataatcctcg ggcagaagat cagcaaaaaa tcaaagaact
    15901 ttcaccaaca ctttctgctt cttatactca aaaagaagat gcgggaacga tttactataa
    15961 tgcaagtgaa tttgcggctc aaccattgat catgataaaa catgaagatg gaacaccgcc
    16021 gaagatcacg aaagaaaaag ttcctgctaa aaaaatgaat gaactgactg tacaagagga
    16081 acttcgaagc tatgaattgc cggaacagca agaaaaagaa atcaaggtag tcagtcaatc
    16141 agaatttgat caactgaact tgccacaatc cactttacag ctagttcagg taaaagactt
    16201 ccaagcaaac ctgaaaccaa tcgaaacact tgttgctcaa aataaaatga acaatccatc
    16261 tttgcaaaaa gtagagtaca gcaaccagaa atatgaaatg tatgaagtgt ataatggtct
    16321 attctctggt tttgaattta tgggattctt cctaggttta gctttcttga ccatgcttgc
    16381 tagctgtttg atgttcaaga tcttgtcagg aagcaagagt gacattgctc gttatgaaat
    16441 gcttgaaaag ataggaacac gacgcgggtt gcttaaacaa tcgatccgta gagaagtagg
    16501 cgtgttattc ttagcaccag gaatcttagg tgtagtccat gtcttattcg gattacaaat
    16561 gtttggtcta ttcatgcaaa atccatacaa agatatttgg gttccgttta cgatcttctt
    16621 cgtattgtat ttcatctact atgtactgac gacatggtta tatactggaa tcgtattgaa
    16681 aaagagattg tgaaaaagct gtcctgcatc aagtaataag aacagccaag caaaaatagc
    16741 tcctcatatt tttcgtttgg ttgggcttat taccgcagat gcaagctttt gaacaccgtt
    16801 tgttcggaaa aagaggttgt gacaatattt ttgtcacaac ctttttttca cggtctttta
    16861 ttagaatata gtaaattttt tctcgttact cacggaataa gctgttaaga aaacggtttc
    16921 catgtatact ggaatcagtg caaatgaaag aaaagagggt gcaaggaaca tggaaatcaa
    16981 tgaacaaaat tttggcgaaa actatcactt gattacgatc acgaatcaaa atgggacatc
    17041 tttgtctgtc agtgatcttg gggcacgggt tgttcatttt caaacaataa tcgataatga
    17101 aaaacgagag ttgatcttag gttttgatga agcaaaagaa tatctagaaa aagaccctta
    17161 tatcggtgct tcaatcggac gtatggctgg acggattacg aatggcacat tcaaattaga
    17221 tgatgaaacg ttcgaagtaa aaacagaccc ggacactggt cattgtttac atggtggtgc
    17281 acctagcttt gaagccaagc agtggcatta tacagtcatc aatggtgaaa acgaagcatc
    17341 ggtcattttc tatcagacta gcccagatat gggaaacgga tttcctggaa ctttagatgt
    17401 agaagtacgg tatacgctga cgaacgatga tacatggcga gtgacggtcc aagggatcag
    17461 tgaccgcaaa acagtattca atcctacaaa tcatgtctat ttcaatttaa ccggtgatcc
    17521 agcacagtca atcgacgaac atatactttg gttgaatagc cgattatttg ctacgctcaa
    17581 taaagataca acgcctacag gagaagtagt ttcagtaagt ggcacagcat ttgacttcca
    17641 acgtcctaaa aaattaggtg aggtctttgc cagtgatttt cgccaatcaa tgatagtggg
    17701 cggcatcgat catccatttt tgttgaaaaa acaatctggt ttactaaatg ctgcggaatt
    17761 gacaagccca gatgaaaaag tatcgatcca agtaaaaaca gatgcttcag gaatcgtgat
    17821 ttttacagca aattttggag aagatggacc ggaaatgcgc gggaataaat tagtaaatca
    17881 tggcggtatc acatttgaga ctcaagaact gccaggagcg gagcgacttg aaagttttgg
    17941 agatgtcact ctggaaccta accaagtacg cgagtttgta acggaataca aaataaatat
    18001 aaagaaatag aataaattaa aaagggagtg tgacagtgtc acatcccctt tttaatttat
    18061 tctatttctt taaaggcaga tcaagccaga atgatttgtc taaacttaac aaggttagta
    18121 caggaacagt tgcagcttta actgtacgag tagttgtttc tggtatctgt cttgccgtaa
    18181 aattgttcca caaatcccag attgaagcga cacggaagat ggaagaagcg ttgttgacgc
    18241 gttttatcgt tgaaaattct aatcctgtga ttggcacgcc atctgttgtc catttgattg
    18301 gcacatcgaa tcgcttagac atattgagga tttcagcgtt tgcttcttca gttgttgctg
    18361 cttctttttt cggttcatca tattttgtca ggtcgtacgc ttcgatgatc gcgttcaatt
    18421 tttgtgaata ttggcgatct gtagcataac gtccttccaa atatttggtt gcttctttgt
    18481 agtcttttgt ttccgacttc caagttggtt tgtagaaatc aggattgcca gtcagtccat
    18541 ttttgatcaa gtcggcatag tcttccaaac tttccttgta agaaggatat ttacggaatt
    18601 gtgcgcggat ttggtagctt tgtccggaac tatcttgttc aagtgtattg aaggtcacgc
    18661 tgcttccttt atatgatcct ttgataccaa aaaggttatg atttggttcg cttgataata
    18721 agctgtttcc agatccactt tcaaggatag cttgagccat catgactgat gcgtagattc
    18781 cttctttttg cccgatttcc tgtgcatcat ctgcaatctt tttcaagaac tgatcagttg
    18841 tttggttttg actgaaatgg aacgagccat ctaatccatc taaaatagat gagccgcctg
    18901 tcagcgaacc tgaagcagca ggtaaacctg ctaagtttac tcggacagca aaatcaggcg
    18961 tgaaatctga aatagaagtt actcgaactt tatctcctgg ttgaggcgca tggataaatt
    19021 gtccgttccc aatatagata cctacatggt gagtaacacc ggaagaaggt ccccagaaga
    19081 cgagatcgcc aggttgggcc tgacttactg gaatctgcgt acctgcatat tgctgttcgc
    19141 ctgtccagcc accgatataa tgaccggttg ctttcatata tacgtaataa gtcaaaccag
    19201 aacagtcaaa agtatttgga ccttttgctc cccatacata tggttttcca agatgcttga
    19261 atgcttctcg aacgattgct tgttggacat tcgttgtatt tgtaggcaat acagcatcaa
    19321 ttggtgattt tacaacaaaa tcagaagaag cagaagatgg attctctata ccgctttgat
    19381 ttgctgtatt tggacgagat gaagcattgt tcgttccgcc attcgatgaa ccattgtttg
    19441 gatggtttgg ttctctctga gtatttccat tgttattcga tccagatggg tttgtttgat
    19501 tcgaagaatt cccgctgttc gaattattgt tattattgtt tgaattattt gaaccattat
    19561 tattgttgtt attacccaca tctggtttgt ttggcgtttc cggatctggt gtactaggga
    19621 cttcttcact aggatcagtt ggtttctctg ttgaagtatc cggtccagct ttaaattctg
    19681 cagtaataga taaattgttc gtacctactg taaaggtctt tgcatcataa tttacttctt
    19741 tggcaccatt aacagcgact gaagcaattt gttcaccttc agctggcgta atagtgtaag
    19801 ttaccttcgt ttccgctaat aaaccttcta ccgttccact ttccgtaata tccaaaggtt
    19861 gctgttcagg ggaatccgct gtcgaagcag tcagttggat cttaccaatc aaagaaggag
    19921 caattgtaag ggaatatgtc tcggctggct tttcattttc tgatggatca gccggatcaa
    19981 tttcagttgg ctcggtcgat cctgttgttg agttagttgt tccagttggg tcttctgtaa
    20041 aagaatccgt tgtttcacta gtagctgagc tggatgtagt cgtatctgtt gtgactgttt
    20101 gatcagctat ttgattatct acggcaaata cggtattggt cgacaaaaca gacggagcaa
    20161 tcagccaagt agcagctagc aaacgtatcg tttttttatt tttcataata aactagtcct
    20221 tttcctttca ttgattttct gtcttattca gcattcatta gacatttttt ggaacagata
    20281 atcgtgtcga aaagcttttt cttttcataa atctgtctgt gtattctaaa taaaacaatt
    20341 tgtaaataag caacgagcat tcattcgttt ttctttgacg ttcattttat tgcatagacc
    20401 acctttgttt ggggataaac aagtggggat aaaaatggtt atgcatatgt aaattatacc
    20461 aaagtttgga aagtatcaca aaaatttata aaaataatga tcctaaaaag aaaaagactg
    20521 aaatatgtga taaacatctc atacttcagt ctttttcatt aactattcat aagacattag
    20581 gtcttcatca aacccgctaa cgagagtagt gatcattgct acgactagac taacagccag
    20641 tcctgctaag taccagaaaa aagtaccgga aatgaatgca ggcaatgtgg tcagactacc
    20701 aaatacaaaa gcgatcgctt tcacttgggc aaatcccatt acagctccac cagcagctgc
    20761 accaatgcaa gcagcaacaa atggtttttt caatgggatc gcaattccgt aaacagttgg
    20821 ttcggtcact cccgcaagta aaatcgaagc aaaagcagaa ccggagatcg aacgggtttt
    20881 tgaacgttta cttcgcagaa atacacctaa agctgcacca gccattccaa aattggctgc
    20941 tcctgtcaac ggaacgattc ggtcgaaccc ataagcagag atattactga tcatgacagg
    21001 gtttacagcc cattggatac caaatattac caaaatattc caaccaccag cgatgattgc
    21061 gccactgatc ggtccgttaa tatccatcat ccaagtaatt gcggcagcta aaccttcgcc
    21121 aaggtaaaca cccgcaggcc cgaataccat taacgtcaaa ggaaccgtga taactaatga
    21181 aagtaaaggt acaaatacta attgcatatt ttcgcgaata tattttttga ggaatttttc
    21241 aacaaaagaa taaaagaaaa tacctaaaat cgccggcatg acagtagacg tatactgcat
    21301 cagtactaca ggaatcccca gaaaatcagt agctgctttg ccactgtctg aaagtaaaga
    21361 ggtcagattc ggttcaatca gtgctgcagc aatgacagcc gcaatatatc cattcacttt
    21421 aaacgttttg gcagcagaaa aagcaagaaa gattggcaga aaataaaaga cggcattcga
    21481 cgcagcagac aaaatatgat aagttccgct tgtttcacta agccagccaa tcgtagtcgc
    21541 taaaacaacc agacctttta atataccaga accggcaaac agcggaatga tcggcgtaaa
    21601 gatttccgat atcaaaccaa atagtttttc gatcggattt agcttttctt tcggagctga
    21661 atgatcgatt tgacgcgtca attccttgta agcaggagcc aatccctgat ccatgattat
    21721 ttctgcatat ctatcgagta aaatgaaata cttaatggaa gggatttgct gttttaattt
    21781 ttctgcattg gctactggct gttttttcaa tttgatacga agccgagtcg cacatggaag
    21841 gacagaatca atattgtctt ttcctcccaa ctgttcaatc agcatatgaa tctgctcgga
    21901 cagtaccatc tgttcaggat tcattgtcgt ttccattaga agaccactcc actcactaat
    21961 ataatattgg cgtaaggtgt catttcgcct gtacgaataa atgcatgaca gttattcaac
    22021 tcttttttca tttcttcatg tgggataaag ctaatcggtg tatctggtaa caataatttg
    22081 attttttcca atatttccgg gttcattgtc tttatttctt ccgccaattc aatcgattgt
    22141 acctcaagtt cttctaaaac gttttctaaa acagataaaa aacttggaag accgtttgag
    22201 acagccaaat ctattttttc agtaccaaag gggacaggca tgcctgcatc cccgatactt
    22261 aatttatcaa agtgccccat ttgtgcgatt actcgtgaaa tatctgaatt gattactttt
    22321 ccttttttca tgtttgtttc ttcctctcat tgttaatttt agactgctga ttgttctaaa
    22381 gcttcgcggt aagggataga aggttgcgca ccataagact gcacagcaac agaagaggct
    22441 agatttccat aaaggatcgc ttctttcaag ttactaaggt ctggctgaag tgtgctgctt
    22501 aatgcgccaa taaatgtatc acctgcggct gttgtgtcaa cggcatccac tttaaaagca
    22561 gggacgatac cgtgttcttt ttctgtatga tagaaagcgc cggcactgcc tagtgtaatg
    22621 attactgttc caatacctaa ttcatggagt ttttcggctg ctgcgaccaa actgttatga
    22681 tctgtcactc tgattcctgt aatgatttct gtttctgttt catttggtat aatgatatct
    22741 gtcttttcaa gtagttcagt aggaatcgtt tcacgagctg gtgcaggatt caggatcgtt
    22801 gtttttcctg cgtcttttgc aatagaaaaa gctaggatcg tcgcatctaa gctactttca
    22861 aactgagcaa taataaagtc actgtttgta atcagttgct tgttttttag gacttcttgt
    22921 tcatggaaag cattatttgc ccctgcatgg atcaagatac tattttctcc tgctgcgtct
    22981 accataatca ttgcttgtcc ggttgtggcg ccttggattt cagctatccc agatgtatcg
    23041 atgttttctt ttgtcagtag atctaataac tgttgtcctt cgctgtcagc cccaacgcct
    23101 ccaataaaag atgtccgtgc gcctgaacgt ttggctgcca ctgcttggtt ggcaccttta
    23161 cctccgccag cgtgaaaaat ctcatgcgca tgcatcgttt cgcctggctt gggcatgttg
    23221 gttaaacgaa gggtagtgtc catattaata cttccaatta ctgtaaccgt gttcatttta
    23281 caaacctcca actatctcat ttattttgat gggaaaatta ttttttaaaa cgttttagta
    23341 aaacgtttta atagttcgat aaaaatatag cacaccttga aagctatttc aagggtgcag
    23401 ttgaaaatct tttttctaat cgtacaggta atcttttttc ttcccattct ttttctggga
    23461 attggatccg atctaacaat aacttggcag atgtttgtcc caattcgaag attggttgtg
    23521 cgatcgttgt cagtttcggc ttgatatact cgcacatatc tatattatca tagccaataa
    23581 tgctgtagtc ttcagggatg ctttttccag cttcctctaa tccgcgatat aatccaaaag
    23641 ctaattcatc atttaaagca aacacaccag ttgcagcaga atcaagaagt tctgctgtga
    23701 tctggtagcc accttgtttt gagaactgtg taggtaagag gatcagttgg tcgtgaggga
    23761 tttggtaaac atcaagagca gatttaaacc cctcaatccg agcgtggaca ttttccggtg
    23821 gattttcagg atagacaagg gcgatcgttt gatgtcctag actaagcaag tgcatgcccg
    23881 ctaggtaacc accgcgaaaa tcgtctgttc gtacggcatc actaaatcct tcagattttt
    23941 tctgatccaa aacaatgaag ggccggcctt gttttttcaa attttcgttg attgcatctg
    24001 tagagacagc agaagtcgcg ataatgaatc catccacacc tcgacgtgtg agctcggcta
    24061 gatattcgat ttctttttga tgatcagaat cagcattaca taatatcgtg acgaaattct
    24121 gcttatacaa gatatcctca attcctcgca tcagtgtact gaagaatgga ttcgtgatat
    24181 caggaacgag tacaccaatt gttttcgttt ccttggtaat catttgtcta gcaaaatagt
    24241 caggctgata gcctaattct tctttggcag ctaaaacttt ctgaacagtt ttcggactga
    24301 atttctccgt atttccattt aatatcaagg agacagttgt gattgaaacg ccggaaagtt
    24361 tagcgacttc tttgattgtg atcttttttt tcaccatgtt catactccta tctaaatcct
    24421 gtggttattt tcccatgaaa tcaaaaagaa gtaaatgaaa atcacgtgtg ccagtactta
    24481 tttgctttgt tggatagctt cgattatttg tttcgttgag cgtactcgtg cagttttcgg
    24541 aaatatcttt tttatgaaca attgatggga ttctttgtca cgatcactgc aagcatcttc
    24601 agcaagcacg atatggtacc cgtgttgaaa ggcatctaat gcagtagcat atacaccgtt
    24661 tgttgtcgct acaccgctta aaatgatagt ttcgattcca cgccggcgaa gttgaagatc
    24721 aagagaagta ccaaaaaaag cacttggatt gtattttttg atagtcaaca tattatctgt
    24781 atctttcaaa gaatccttca aaagtaaatc agtgaattct ggaggtacag aagcagtcat
    24841 tccacccata tcagtttgtt gactcaaata agggaaagtg gagatgtcta cgttcactaa
    24901 aactttcaag gcagcggtac gtttcaatgt tcttactaac aaatcattgt ttgtcagaat
    24961 agatgctgca gtgtgtggag agagtgtacc gttatttacg atctcttttt gcatgtcgat
    25021 cgcgacaaaa gcagtttttg ataaatcaaa aatcatattt tcctttaaat cattcatgat
    25081 gttccttctt tcaaataaca atgtttttta tgtaagtaaa tcataaatcg tttatttgtc
    25141 aactaaatta aaggattcct ttctttagtc tagagaaatt cagtggtaaa ataaataagt
    25201 gtgagaaaac gcattcgatg catagatata gcgagaaata tcgtgaaaat ggaggaatca
    25261 gagtgaagta tgtcgaagta aaaaatgaat acaaacgata caaaatggga gaaacgacca
    25321 ttacagcaaa tgacgatatt tctttttcaa ttgaaaaagg cgaattagtg attatacttg
    25381 gtccaagtgg agcaggaaag tcaacgattc ttaatatttt aggtgggatg gatacacctg
    25441 atgaaggtca gatcattatt gatgacacag atattgcgca attttcagat aaacagctta
    25501 ctgcttaccg acgaacagat gtagggttcg ttttccaatt ctacaatctc gttcctaatc
    25561 taacagcaaa agaaaatgtg gaattggcaa cggaagtttc tcctgatgcg cttgatcctg
    25621 tagaggtatt gaggcaagtg ggcctagctc atcgattgaa taactttccg tcccaattat
    25681 caggaggcga acagcagagg gtttcaattg ctcgtgcact agctaaaaat cccaaacttc
    25741 ttttatgtga tgaaccgaca ggcgcgctgg attttgaaac gggcaagcaa gtattgaaat
    25801 tattgcaaaa cgctagcaga caacacggca ataccgtttt gatcattaca cataattctg
    25861 ctttagcgcc catcgctgat agagtcattc acattaatga tgctaaagta aggtctgttg
    25921 aattaaatga tcatccttca tccattgatg agattgtatg gtagggggat tttatgaaaa
    25981 caagaacata tcttaaagca ggttttcgcg aaattcggca atctaaagga cgattcattg
    26041 caatcatcct tattgtcatg ctaggaacac ttttatttgt aggtgtcaaa actgctggac
    26101 ctgtcatgca aaaaacgatg gatcactatg ttggtacagc aggactttct gatttgcaaa
    26161 tcgtttctac tggtggcttg acacaaaaag atattgctca agcagaaaag atcccagatg
    26221 caaaagtaga aacaggaaaa cagatctatt attccaatac tggcaaaaac gaagtgattc
    26281 aaatattttc ttataacaaa aataacaagc agaacaagct acaattaact gatggaaaac
    26341 tcccggaaaa accaaaccag cttgtattgg atgaaaaagc ggaagatgaa gggtataaaa
    26401 tcggtgatat ttatcagatt gattcagatg aattgaaaga aaaagagtat accatcacgg
    26461 gctttgtccg ttctccattg ttcatcaaca atttggagag aggatacgcg aacgtcggaa
    26521 atggtagcgt cgattatttt gtctatttac ctgaatccgt ttttaaaaca gatatttatt
    26581 ctgtcttata tcttgatttt acaaacgtaa agaatttgaa tacgtacagc aaagcttaca
    26641 aagataaaat ggagcaaaat caagaaaagg cagaaaagta tttacaagat cgacccgaag
    26701 aacgcttaga agagctgaaa aaatcggcca acgaagaatt agaaccggca cagcaaaaaa
    26761 tagcggatgg caaagcacaa acaacgcaag caagaacacg gttagaagat gctaaaaaac
    26821 agttagcgca acagtcagca actatccagc agcttcctga agcgcaacga acaatcgctg
    26881 ttcaaacatt acctcaacaa gaagctcaac ttgctgaaca agaaaggcaa ttagttgaga
    26941 aagaacgtga actggcctct gcagaggaag aattgaatcg aaatgcagaa aaaattgaaa
    27001 atttaactaa accatcatac ctatacaatg aacgttcaga aaatgtcggt tttcaagaat
    27061 ttggtgatct gtctgatcgt atcgctgcga ttgccaatgt ttttcctgtt ttctttttct
    27121 ttattgccgc attgatcacc tttacgacaa tgacacgtat ggtagaagaa aaccgcaggg
    27181 aaatcgggac attaaaggcg ttaggttata cgaaagttga aatcgcaaaa aaatatgcaa
    27241 tctatgcaag tctagcctca gggataggta ttgttttagg aacgatatta ggaacgaatc
    27301 ttttgccaag gatcattttt gagctgtcta acgagcggta tgatattggg agtgcgctta
    27361 ttttttatga ctggccgccg attttacaag cagctgccgc attcttcatt gccgcatttg
    27421 gcgctgcaat gatcgtttta ttcaaagatt taagagaacg gcctgctgca ttgcttcaac
    27481 cgaaagcacc gaagccaggg aaacgcattt ttcttgaata catcactccg ctgtggtcac
    27541 gccttagctt taatcaaaaa atcagttacc gaaatctctt ccgatataag tcaagaatgc
    27601 ttatggcgat tatcggaatt gctggatgtg ccgggctgat ggttgcaggc gtgggattga
    27661 aagattctct aagttctgtt tcagccaaac aatttggacc gatcattgac tatcaagcaa
    27721 tcgttacatt ggattcatcc acggaagata gtcgtcaaaa agcagcggat atgctgcaga
    27781 agcaagaaaa agtaaatgag atcctagcta ttcatactca aaccgtagaa ttgagaaaaa
    27841 aaggacatgc ttcgcaaagt ctgacagtga tcgtaccaag taaaacagct gatttggatc
    27901 gttttatcca tttaactgat cttgaagaga acccacgctc cttgcctaaa acaggaatcg
    27961 ctattacgca aaaagtagca gaactatttg acttatctac aggagatcat atttcgcttt
    28021 atgatgaaga tcaaaaagaa tggaaagtcg aagtaaaaga aatcgtgaaa aactatctag
    28081 gtagctttat ttatatgtcc cctgaatatt atgcttcagt tactgatcaa tcgatgatgg
    28141 ataatgcatt tttgctgcgt acagataaga tgacaacaaa agaagaagaa atgctttccg
    28201 agaaactgct tgccacagac gctgtgacaa atacgtcttt catgtccaaa caaatcgaaa
    28261 cgcaagaaga atcaatggcg aacttagact cggttgtctt aatctttgtc gtattatcag
    28321 gactgctggc ttttatcgtc ttgtataatc taactaatat caatatttcc gagcgtgtac
    28381 gcgaattatc tacaataaaa gtccttggat tttttgataa agaagtcacg atgtacattg
    28441 ttcgagaaaa tatcatcttt actcttttag gaatcatcgg aggatttggt gtaggatata
    28501 tattgactga cttcatcttg caacaagcgt ctatggagaa tgtcatcttt ccattagtga
    28561 tcacatggtg ggcttatgtc ttatcagcag gattaactat cgtatttacg gttattgtga
    28621 tgattgttac ccatttcaaa ttaaagcata tcgatatgat tgatgcgttg aaatcgaatg
    28681 aataaaaaca gaggttgtga aatcagcgtt ttgagctaat aacgagggtc agctgctaat
    28741 tgaacaccat gaatcagagg ttgtaaaaaa gccgtcctgc atcgagcaaa aagaatactc
    28801 aaataaaatt agctcctcat gttttacatt tgagaagact gattgccgta gataaaagct
    28861 tttgaacacc atttatagaa gaaacaagaa tattttgcta ttttaaacaa gaggatagac
    28921 aaatacatgt ctgatctctt gttttttatt gggcaacaga cctcttagaa tgtataaaga
    28981 taaattttac aaacgctttc tgttcagtta taataataaa tgataacttg tatatataag
    29041 ctgtggtagg taataaatca gttgaatgga ggtaaatatc catgacattt aaagacaaag
    29101 tcatttacca aatctatccc aaatcttaca aagacagtaa tggtgatgga attggagatt
    29161 taaaaggaat caaagaaaaa ctcccgtatc tgaatgatct aggcatcgac atgatttggc
    29221 taaaccctat ttatccaagc cctcaaaaag acaacggtta tgatatctct gattacacag
    29281 ccatcgatcc tatctttggt acgatggagg aattcgaaca gctaatagcc gaagcaaaga
    29341 aataccggat agaaatcatg ctcgatatgg tgctgaatca cgtttcgaca gaacatgaat
    29401 ggttcaaaaa agcactagca ggggaccagc attatcagga cttcttcatt ttaagagaag
    29461 agccaacgga ttgggtatca aaatttggtg gaagtgcttg gtcgccattt ggagatacgg
    29521 ggaaatatta tttgcactta tatgatcgga cacaagctga cttgaactgg cggaaccaag
    29581 cagtaagaga ggaactattc aaagttgttc gtttttggat ggaaaaaggg gtaaaaggat
    29641 tccgtttcga tgttatcaat gttatcggta aagacgaaca attgaaaaat aataaagaaa
    29701 atgatggcaa acctgaatat acggacaaac caatcaccca tacgtatcta aaagaattga
    29761 accaagcaac gtttgggacg gattccacta tcatcacagt cggtgaaatg agctctacga
    29821 cgatcgaaaa ttgtatcttg tatacagaac ctagtagaga agaattatca atggtcttta
    29881 atttccatca tttgaaagtc gattatgaaa atgggaataa atggtctact cctccatttg
    29941 attttgcaga attaaagcga ctatttcata catggggaga aaatatgagt gagggtaacg
    30001 gctggaatgc tctatttttt aataatcatg atcagccacg tgcgctcaat cgatttgtag
    30061 ttatcgatca gtatcgaaaa caaggagcag agatgctagc aaccatgatc catttgaacc
    30121 gtgggacgcc ctatatttat atgggagaag aaatcggcat gatcgaccca gactattcat
    30181 cgatttccga ctatattgac gtagaaagtt tgaatgctta taagttactc cttgaacaag
    30241 gattcacaga aaaagaagcg tttcgtcgga tcaaagcaaa atctcgcgat aactctcgaa
    30301 cacccatgca atggtcagac acagaacaag caggatttac tacagggaaa ccttggttaa
    30361 aagttggagg caagctagaa gagattaatg tagaaaaaga actatcttct gaagattcta
    30421 ttttgtccta ttacaagaaa ttgattcggc tgagaaaaac atatccaatc gttgcacaag
    30481 gagattatca tgcttatgaa gcaaatcatc cggaagttta tggctttctt cgccaatttg
    30541 aaggacagca gctgcttgta ttaacaaatt tctatgataa agatacaagg atcgccatcc
    30601 cagaaggatt cacagaagcc aatattctta tcgataatta ttccgatact gttcttcatc
    30661 aaagtctgga gctaaaacca taccaggcaa ttgctttgct gaaaaattaa aacaaaaaca
    30721 caaaacgtcg ttcctacgag ctattctcgt aagatcgacg ttttgtgttt tatttaaaat
    30781 aatcttcttc agaaaatgaa gagttttcat actctccttt atcgccataa aatttattgt
    30841 agcgttgttt gaatttacgg aaaataaaag taacgagtaa tttgactaac gtatagatag
    30901 gaataccaaa aataacgccc acaattccca ttagatcacc catgacaagt aaaacgatta
    30961 agatcgtaat cggatggatc tgtaaacgat cacccattac tcgaggaatc accaagtcac
    31021 cgtggagaag ttgtacggca aaccagacaa tgacaaattt gacagccata aaagtagagt
    31081 cttggaatgc aatgatcaaa cctggaaaga aggcgataaa cggcccaata tatggaatga
    31141 tacataacaa cccagcagaa atagctaaaa cactagcaaa ttctaatccg atcagtaaga
    31201 aacaaaccca gtagactgct ccaaggataa atgaagcaat gatttgcccc tttagaaatg
    31261 aacccaattg ttgattagca atctttgtca aatttttaaa atctgtacga aatgctggtg
    31321 ggacaatcgc taaaactgac tgataaaatt tttgaggatc tctcaaaagg aaaaaagcga
    31381 caattggacc agtaaataaa gtaataaaaa tagctgtaac agttgaaaaa atgttgctga
    31441 tccgttgtgc gccactttcc caataatttc caataaatcc agctatatca gacgtcacgt
    31501 cgtcaataga agcaaaaaat tgattaaatg aatcggcaaa aggtgttttc tccaaaaatt
    31561 gtcggagact ctcttgaaaa tcttgaaaca aatctggcaa actactgatc aatgtctttg
    31621 cctgttcttc aagaaaagga ataagccata tgcttccaag ccccaataaa aataaaataa
    31681 tcacataaac gataatgact gacaagttcc tcgaaaggtg cttgctcaaa cgtttgatga
    31741 ctggttccaa aagataatac aagatcagtg caaacaataa tgggggcaag atagtagcga
    31801 taatgattcc gacaggcttg aatataaacg agatctgatg gaaaaaataa atgatcagcg
    31861 caagtaagat aactattcct aaaacatagt aagaggtttt tcctcctaaa aagcggatca
    31921 aacgattcgt tgtatctgtt tcttttttca ctctttttcc tcctttatct ataacttcat
    31981 tatagacaaa agcgggtata aaaggaaaat gatagaggtt gattgcaaaa atatcgtcaa
    32041 tcattcatat aaactttctc atcaattaaa ataagaaaac gcttgacagt tcttttaacc
    32101 atatgataaa ttaaatcctg ttgctaaaac gtaactatta cggtttgcga aaagcgaatc
    32161 ggaacattca aatgaaagaa gatgagaaga ggagtgtcat aaatgaaacg attgataggt
    32221 atattggcca tgctggtttt agcaggtatg ttaactgctt gcggggcaag tgggaaagcc
    32281 gaggacagta aagaaaaatt gagcgtgatg acgacctttt atcccatgta tgattttaca
    32341 aaagcaatcg tcggggatga aggagaagta gagttattga ttccagcagg gactgattcc
    32401 cacgattatg aaccttctgc taaagacatg gcaaagatcc aagatacaga tatctttgtt
    32461 tataatgatg aaaacatgga aacatgggtc cccgcaatcc aaaaaacatt acaagaaggc
    32521 aatgtccata cgataaaagc gacagaaggt atgcttttac ttccgggaag tgaagaagga
    32581 catgatcatg accatgaaca cggggaagaa ggccatacac atgagctaga cccacatgta
    32641 tggctagctc caagtttagc aatcaagcag gtagcaaaca tacgtgatca gctgattgaa
    32701 gcttacccag aaaaacagga agtatggaca aaaaatgcag cagcctatac cgagaaactt
    32761 caagcactcc atcagcttta tcaagaaacc tttaagcaag caaaacaaag atcttttgtc
    32821 acgcaacatg ctgcattcaa ctatctagcg ttagaatatg gattgaatca agtatcgatt
    32881 gctggtcttt cttctagtga agaaccgtct gcggcgagaa ttgctgaact gaaacacttt
    32941 gttaaagaac atggaatcaa ctatatttat tttgaagaaa acgccaaaga tagtattgcc
    33001 agaacgttag caaacgaagc aggtgtatct ttagaagtat tgaatccttt ggaaggattg
    33061 acgaatgaac aaatagaaaa tggagaaaac tatctttcta tcatggaagc aaacctcgaa
    33121 gcgttgaaaa aaacgacaga gacagaaaac ccgctagaag atactttgac ccccaaaaaa
    33181 gaaaagagcg tctacaacgg ttattttgaa gacgaggatg tacaagatcg ctctctttca
    33241 gattgggcag gaacttggca aacagtagat acgtatgtag aagatgggac ttttgatcct
    33301 gtttttgaat ataaagaaaa attgaatcag gataagacag cacaagaata taaagaatat
    33361 tatacgaagg gctatcaaac agatattgct tcaatcaaga tcagtgagaa agagatggca
    33421 tttacgttta aggatggaac aacgaaacgt tcaaattatc gttatgtagg gaaagaaatc
    33481 ttgacctacg aggcaggaaa ccgtggcgta aggtatctat tcgaagcaga agataaggaa
    33541 agtggcgctt ttagatatat tcaattcagt gatcacacga ttgcaccggc tgactccgaa
    33601 cattttcatt tatatgcagg aaatgaaagt caagaagtat tgctgaaaga aatggagaat
    33661 tggccaacat tttatccaga tacaatggat ggacgtgaaa ttgcacagga aatgcttttg
    33721 cactgatagg caaaaa
//
LOCUS       DACDXO010000026        31834 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E23605_2017
            SAMN12639989-rid9881463.denovo.026, whole genome shotgun sequence.
ACCESSION   DACDXO010000026 DACDXO010000000
VERSION     DACDXO010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639989
            Sequence Read Archive: SRR10023389
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31834)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741914.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:42:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,972
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 33 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 17 of 73
            Pseudo Genes (multiple problems)  :: 10 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31834
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639989-rid9881463.denovo.026"
                     /isolate="E23605_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(151..2304)
                     /locus_tag="IWM35_001408"
     CDS             complement(151..2304)
                     /locus_tag="IWM35_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HAP9919268.1"
                     /translation="MMKDFMKKILNTSWLEKIRKGAQEPKNKTFRRSHVPFRLNFLFF
                     IIFTLFVALIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKALVTNKA
                     NQAITYTRGTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHLKEATKR
                     LSAKEKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVYIKNSGV
                     TDQELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADEAEKYLA
                     KGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGSNLMLSM
                     NAEFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHEQGSKEI
                     TENALGTITSAFDPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKSSVFNRS
                     GQVALDAVKASELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRDSFKEFG
                     LGTTTGIDLPNESPGISRSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYTPIQLAQ
                     YASTVANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMAILREGF
                     HQVVHGTDPYTTAKPLASAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGPTDNPEI
                     AISVVLPNLLDEQDHINLTIAKEIMDAYYDMFMAK"
     gene            complement(2598..3446)
                     /locus_tag="IWM35_001409"
     CDS             complement(2598..3446)
                     /locus_tag="IWM35_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP9919269.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAIQE
                     YFMDSK"
     gene            complement(3670..5610)
                     /gene="thrS"
                     /locus_tag="IWM35_001410"
     CDS             complement(3670..5610)
                     /gene="thrS"
                     /locus_tag="IWM35_001410"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HAP9919270.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(5947..6126)
                     /locus_tag="IWM35_001411"
     CDS             complement(5947..6126)
                     /locus_tag="IWM35_001411"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HAP9919271.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(6173..7996)
                     /gene="cadA"
                     /locus_tag="IWM35_001412"
     CDS             complement(6173..7996)
                     /gene="cadA"
                     /locus_tag="IWM35_001412"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HAP9919272.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            8274..10553
                     /locus_tag="IWM35_001413"
     CDS             8274..10553
                     /locus_tag="IWM35_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP9919273.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
     gene            complement(10646..11293)
                     /locus_tag="IWM35_001414"
     CDS             complement(10646..11293)
                     /locus_tag="IWM35_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9919274.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLYIE"
     gene            complement(11472..12191)
                     /locus_tag="IWM35_001415"
     CDS             complement(11472..12191)
                     /locus_tag="IWM35_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAP9919275.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(12322..13473)
                     /locus_tag="IWM35_001416"
     CDS             complement(12322..13473)
                     /locus_tag="IWM35_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HAP9919276.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(13690..14430)
                     /locus_tag="IWM35_001417"
     CDS             complement(13690..14430)
                     /locus_tag="IWM35_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP9919277.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(14434..14757)
                     /gene="rsfS"
                     /locus_tag="IWM35_001418"
     CDS             complement(14434..14757)
                     /gene="rsfS"
                     /locus_tag="IWM35_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HAP9919278.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(14814..15407)
                     /locus_tag="IWM35_001419"
     CDS             complement(14814..15407)
                     /locus_tag="IWM35_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP9919279.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(15391..16038)
                     /locus_tag="IWM35_001420"
     CDS             complement(15391..16038)
                     /locus_tag="IWM35_001420"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HAP9919280.1"
                     /translation="MNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHLV
                     MAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIELA
                     RGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPGY
                     TTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(16051..16362)
                     /gene="yhbY"
                     /locus_tag="IWM35_001421"
     CDS             complement(16051..16362)
                     /gene="yhbY"
                     /locus_tag="IWM35_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HAP9919281.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(16378..17487)
                     /gene="yqeH"
                     /locus_tag="IWM35_001422"
     CDS             complement(16378..17487)
                     /gene="yqeH"
                     /locus_tag="IWM35_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HAP9919282.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNETQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(17484..18011)
                     /locus_tag="IWM35_001423"
     CDS             complement(17484..18011)
                     /locus_tag="IWM35_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HAP9919283.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(18191..18976)
                     /locus_tag="IWM35_001424"
     CDS             complement(18191..18976)
                     /locus_tag="IWM35_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HAP9919284.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(18969..19838)
                     /locus_tag="IWM35_001425"
     CDS             complement(18969..19838)
                     /locus_tag="IWM35_001425"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HAP9919285.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(19839..21218)
                     /locus_tag="IWM35_001426"
     CDS             complement(19839..21218)
                     /locus_tag="IWM35_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HAP9919286.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(21226..21645)
                     /gene="fabZ"
                     /locus_tag="IWM35_001427"
     CDS             complement(21226..21645)
                     /gene="fabZ"
                     /locus_tag="IWM35_001427"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HAP9919287.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(21670..22146)
                     /gene="accB"
                     /locus_tag="IWM35_001428"
     CDS             complement(21670..22146)
                     /gene="accB"
                     /locus_tag="IWM35_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HAP9919288.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVIPEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(22149..23384)
                     /gene="fabF"
                     /locus_tag="IWM35_001429"
     CDS             complement(22149..23384)
                     /gene="fabF"
                     /locus_tag="IWM35_001429"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HAP9919289.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(23403..24140)
                     /gene="fabG"
                     /locus_tag="IWM35_001430"
     CDS             complement(23403..24140)
                     /gene="fabG"
                     /locus_tag="IWM35_001430"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HAP9919290.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(24152..25069)
                     /gene="fabD"
                     /locus_tag="IWM35_001431"
     CDS             complement(24152..25069)
                     /gene="fabD"
                     /locus_tag="IWM35_001431"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HAP9919291.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEAAPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(25176..25400)
                     /locus_tag="IWM35_001432"
     CDS             complement(25176..25400)
                     /locus_tag="IWM35_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HAP9919292.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(25453..26415)
                     /locus_tag="IWM35_001433"
     CDS             complement(25453..26415)
                     /locus_tag="IWM35_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HAP9919293.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(26425..26865)
                     /locus_tag="IWM35_001434"
     CDS             complement(26425..26865)
                     /locus_tag="IWM35_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP9919294.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
     gene            27171..28136
                     /locus_tag="IWM35_001435"
     CDS             27171..28136
                     /locus_tag="IWM35_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HAP9919295.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRVQLPDLQ
                     EMTFEQKKKELQGLFQGRNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKQFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMITSGDVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQFEIELLNKKEAEKQLN"
     gene            complement(28193..28423)
                     /locus_tag="IWM35_001436"
     CDS             complement(28193..28423)
                     /locus_tag="IWM35_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HAP9919296.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(28509..29396)
                     /locus_tag="IWM35_001437"
     CDS             complement(28509..29396)
                     /locus_tag="IWM35_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HAP9919297.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            complement(29485..30708)
                     /locus_tag="IWM35_001438"
     CDS             complement(29485..30708)
                     /locus_tag="IWM35_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAP9919298.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            complement(30725..31804)
                     /locus_tag="IWM35_001439"
     CDS             complement(30725..31804)
                     /locus_tag="IWM35_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAP9919299.1"
                     /translation="MLYYISEGGRKVKVLLYFESEKMLAKSGIGRALDHQKRALTEVG
                     ISYTLDEKEDYDILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIGSNQL
                     SPIVKKYLVGLYQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQ
                     KFREYFKLSPDQKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIR
                     KIVKEDHPENVLFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVR
                     DIPVYNGWLEDSKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEE
                     LKNVYESVLNEKVSSKLKKVNQVKD"
BASE COUNT         8793 a         6969 c         5488 g        10584 t
ORIGIN      
        1 aatttttgtc acaacctttt ttgccgagta aacggtgttc aaaagccagt ttctcggtat
       61 gaagtcaaat ttaagcaaaa atgtgagaag ctattttcac aaaatttttc ttcatactcg
      121 aaactaagcg gctttttcac gacctctttt ttattttgcc atgaacatat cataatacgc
      181 gtccattatt tctttagcaa tcgtcaagtt tatgtggtct tgttcgtcta ataagttcgg
      241 caagacaaca ctgatggcaa tttccggatt gtctgttggt ccgtatgcca cgatattact
      301 attgactgtc gcaatatctg gatgaccttc cgcaaccgtt tctgcagtcc ctgtttttgc
      361 ggaaaggtcc attttcgcac tggctaatgg tttggctgtt gtgtaaggat cggtaccatg
      421 gacaacttgg tggaatcctt cacggaggat cgccatattt tctgcagaaa tattgaccgt
      481 attttctact gttgtgccaa tttctttctt cacttcaccc aagtttccat tttcgtcatt
      541 gccatagatc gctttgacta ggtgcggctg tactcgtttc ccgccattag cgactgttga
      601 agcatattga gctaattgga ttggtgtata tgtgtcaaac tgtccaaatg ccaaatcggt
      661 aaagttacct ggagcatacc attctttgcc attatccgca ttgaacttct tcatataatc
      721 aacagaacgt gagatcccag gagattcatt cggcaaatcg atcccagtcg ttgtaccaag
      781 accaaactct ttgaaagagt ctctcaattg ctgataagct ttagcttctt catctaaact
      841 aggtagtccc attccaggtg tatagtctag tcctaacatc tttaacgcca ctttgatcat
      901 atacgtatta gaagacaatt ccgatgcttt gactgcatca agagcaactt gtccgcttcg
      961 attaaagaca gagctttttt ctgaagcacc ttgcagacga atcggctcat caatcaaaac
     1021 ttgatttccg ctgatcgcgt tacttgccca gcctgctgtc aaagtaccag ccttgactac
     1081 agaccctgga tcaaaggcgc tagtaatcgt gcctaaagca ttctcggtga tttctttgga
     1141 accttgttca tgactgaaac cagtcattgc tagcacttct cctgtctgag gattcatagc
     1201 cactgcataa gcaccaggcg aatactgtgc tttgccgttg ttgatcaatg tttgataatt
     1261 ccttttcaaa atttcttcta cttttgattg gaattctgca ttcatagata acattaaatt
     1321 agaacctttt tccccagaga agatttcttt ttgattcgaa acatttcctt cattatcaag
     1381 ggaaacttca tattgggttt tggttccttg caggacatct tcatattgtt tttccaaata
     1441 actttgtcct acacggtcat tccgagaata accttttgct aggtatttct ctgcttcatc
     1501 tgctggaagt ccttgctttt cagtcgtcac actgccaaga atacttttca gtgaacttgc
     1561 tgcattatat tctcgtgtcc agtctgtgcc agtcgaaact cctggaagtt cagaagcgcg
     1621 ttctgccaca acagctaact cttgatcagt aacaccagag tttttgatat agacagtgtt
     1681 taattcatat gcactattca tccgtttaaa gatggtagca atttttaatt gctcctcgtt
     1741 taaattaatc tcatcagctg tgactttatt gaccatcgca ctatattgtt cgctgttccc
     1801 taattgcttt tcttttgccg aaagtctttt tgtggcttct ttcaaatgtt tgggatcagc
     1861 cagccaaaaa tcttttttat ctctatctgt cagattttca tctaccggaa catcgatcag
     1921 cgagttcagc ttcgttgcga tttcaagcaa atctttagcc gtaacttgtg ttcctctcgt
     1981 atatgtgatg gcctgattcg ctttattcgt aactaaagct tttcctgtgg agtcgtagat
     2041 cattccgcga ggagtacttt cttggaccgt aatcgtagaa gtagacttca ctcgcgcctc
     2101 catctcttcg ccgttcacga tctgcaaata acccaatcta gcaatcaacg caacaaataa
     2161 cgtaaatata atgaaaaaaa gaaaatttaa gcgaaaagga acatgggacc ggcgaaacgt
     2221 tttgttctta ggttcttgtg caccttttct tattttttct aaccaagaag tatttaatat
     2281 ttttttcata aaatctttca tcatttccta tccttccgcc tatttacatt catgcttcat
     2341 tgtacccaaa aacttcttaa taaaaaagaa aaaatgggcg gagcaagaaa ttctttacct
     2401 aatttcaaga taaaatcaac tttttgtttc acagcaatca cttaaagtta catatccaac
     2461 tatttttttc attaaaaaat aaacttattt ttgaaaatta tgttttctta agtcgtgaaa
     2521 cactttgatg gatcatttat ttgtggaaaa ctacgttttg ttattctaaa aaacttatat
     2581 aaaaaatagt aaggttttta tttcgaatcc ataaaatatt cttggattgc ttctcgtgtt
     2641 tttttcactt tttcgctaag cttttcgttt ttagccatat agaaataaaa aatgatatcg
     2701 atcgtagcaa attgggccaa aagtgaatta gttgccgcac ttcgattcgt gatttctttt
     2761 ggtctagatg tttgtacgag tacatcggct atcttgctaa gcgggttatt tccaaattgc
     2821 gtcaacgcaa tgatttcgat attcatgctt ttcgccagct cggctaatgc gactacatct
     2881 gcacttctac ctgagtttga aacaagccaa agcactttct ttttctcatt gctgacaagt
     2941 tgcgggagca agacgtggat atctttttca aaaatgattg gcttaccgac acgagaccat
     3001 ttttgcagaa tatcttctac tactaaagaa gaggcgccaa ccccatataa aaaaacagtc
     3061 tctgccgatt ccaaacactc tacgactttt tggatcgttt cttcttctag aatatctact
     3121 gtttctttta tcgctacttg tgcattgtta ccaagtttgt ttttgataga gtaaaaggac
     3181 tcatttgctt ctatatcact aaaaccttca tcgattccag tctgtagttc tgttgaaatc
     3241 gtgattttga agtccgtcag actttgaaaa ccgatttttt tggaaaaacg tacaactgtc
     3301 ggcgcactca acccggaagc ttctgctaat tcattagctg tcattgtagg gaccttctca
     3361 atattaccga taatgtagtt ggccaacttt ttttctgctg aagaataatc ttccatcata
     3421 ctttcaatac gactaattac gctcataagt tcctccaccc ttttaatgat agtctatgta
     3481 agtataccat atcagaccct tattcacggt cgtatactgg ataaaaaaca aaaaaacaat
     3541 ccgcccaaca gaatatctta tcccatcaat cccacagatt ctattgagca tctgtgtctt
     3601 tgaggaatac ctgttatcta ttgttcggat tgctttctat ctactacttt tttacaggaa
     3661 atttacctat tagttttctc tgctgtaatt tttgacttct gcttccatag catcaataaa
     3721 gatattcaaa tcttctacag ctgtttcttt acttccataa cgacgtacgt tcactgttgc
     3781 atcttccaat tccttgtctc caacaactaa ttggtaaggg atcttttgtg tttgagaggc
     3841 acgtatctta tagcccattt tttcattacg atcatcaact tctacacgca accccttcat
     3901 ttgcaaacgt tctttgattt cataagcata atcgccatgt gcatcaacag aaacagggat
     3961 gattgttgct tgtataggtg ccaaccatgt tggaaaagcg cctttgtata cttctgttaa
     4021 ataagcgaca aaacgttcca ttgtagaaac aattccgcgg tggataacta ctggacgatg
     4081 cgtattttcc ccgtcttctc caacatatgt caaatcaaag cgctctggca ataagaagtc
     4141 taattgtata gttgataatg tttcttctgt accaagagct gttttgactt gaacatctag
     4201 ttttggtccg taaaaggctg cctcgccttc tgcttcaaag taatctagtt ctaattcatc
     4261 catagcggct ttcagcattg cttgggcttt ttcccacatt gcgtcatcat caaaatattt
     4321 atctgtattg tttggatcgc gatagctcaa gcggaaacga taatctgtaa tattgaagtc
     4381 agcatatact gccaccatca attcaagcgt acgtttaaat tcatctttga tttgatcagg
     4441 acgaacaaat gtatgaccat cattcaacgt catttctcgt acacgttgta aacctgacaa
     4501 agcacctgat ttttcatagc gatgcatcat tcctaattca gcgatacgaa ttggtaattc
     4561 acgataagaa tggatcgtgt ttttgtagac catcatgtga tgagggcagt tcattgggcg
     4621 aagaacaagc atttcgccat ctcccatatc cattggcggg aacatgtctt cttgataatg
     4681 atcccagtgt ccagatgttt tgtacaactc aacatctccc attattggtg tatatacatg
     4741 ttggtaacct agactaactt ctttgtctac gatataacgt tcgatcgtgc gtcggattgt
     4801 tgcccctttt ggaagccaga atggcaaacc agaacctact tctggggaaa ccatgaacaa
     4861 gtcaagttct tttcctagtt tacgatggtc tctttctttt gcttcttcac ggagacggat
     4921 atattctttc aaagctttct tgtcaaaaaa ggctgtcccg tatacacgtt gcatcatttg
     4981 attgtttgag tttccacgcc aataagcacc tgccactgat aataatttaa atacttgtat
     5041 acggccagtg gatggaacat gtggtccacg acacaaatcg atgaattcgc cttgttgata
     5101 agcagtaatc acttcttctt ctggcaattc actaatcagt tctactttgt acggatcact
     5161 tgcaaaaatt tctaatgctt cctgttttga aagaacacgg cgttcgatcg gcagattttc
     5221 tttgacgatt ttcatcattt ctgcttcgat tgccggcaaa tcttctgcag ttacttggtt
     5281 ttgtccatta tctgtatcgt agtaaaaccc tgaatcgata gctggtccaa cgccaaagtg
     5341 gatatttgga tagaggcgac gcatggcttg ggccatcaaa tgagctgctg aatgacggac
     5401 taagccaagt gcatcttcat ggtctggtgt gatgatctca atacttgcat cttcctcgat
     5461 tggacgagtc aaatcaacga gttttccgtt cactttacca gctaaagctt tttttgctaa
     5521 gctttttgaa atgctttcag caattgccaa tgttgttgtt ccagactcaa attctttgac
     5581 tgcaccatct ggaaaagtaa tttttatcat aatagtttcc tccttgaaat gttttgattg
     5641 atcagatcat caagctgaaa aacaaaaaaa tcctagcatc tcttgaaaag agatactagg
     5701 acgaaaagtt cgtggttcca cctaatgttc aaacgcaaat gcgttcctca agcgttgtaa
     5761 cggtccgacc gcccttgctt caacaagggt ttcgaaagtg gtcatccttt ggttttctcc
     5821 tggaaaagtt tcagcctagc ttttcctctc ttatcgaaga ctctcaaatt cagttggctt
     5881 tctcatcaat caattatgtg tttatttaac cactaataaa tagaaaaatc aagtgggaaa
     5941 ctaaacttat tttttccatt ccccatttac accgtatgtt ccttttttca tctcattgat
     6001 gatgacatgg atgtgttctt ttggtgcctg cgtgttttta tgaacagctt ccgtgatatc
     6061 tttcatcata ttttctaatt gttccggact gcgaccttct actaattcga catgtacgaa
     6121 tggcataatg ctctctcctc tttctgatcg ttcttttttc aactcgattc tatcatacta
     6181 tgtttgtctt gggtactgaa tcaggaaaaa tggctggatc tttttttgct ctcaaggcac
     6241 tcaaaataga gaccgtatcc acgacttctt gcaaaacagc tccgaataat gcggggatta
     6301 gtcccgtact tgcaattaac atcaaaaaaa tacaaataaa gatgccaatc agtacagact
     6361 gtttcgctat tctcatcgta tctcttgaaa tcactactgc ttttgctaca cgcaagagat
     6421 catctttcaa gatgaccgca tctgcacttt cactagcagc tgtagaacca tgagccccca
     6481 ttgcaatacc gatatctgca gcagcaagtg ctggtgcgtc attcacgccg tctcctacca
     6541 tcgcaacagg tctttgatct ttagggatac tttctaaaat ggtcagttta tcttgaggca
     6601 agctacgggc atgtacagaa tctaattgga ctttttctgc aatcgtttga gcaatggcag
     6661 atccatcccc ggtgatcatc atcaaatttt tcacatcaag ttttcttagc tccttgattg
     6721 tctttgctgc ttctgggcga atctgatccg aaaataggat cgttcctgca aaagatcgat
     6781 caattgttac atagattgct gtattggacg tttctatttc ttcagctgaa acaaaagagg
     6841 cacgtccaat ctttacttca tgattattga ctactgcttg gattccttga ccggtcactt
     6901 ctttcagttg actaacagga tacagttggc tgtttttcac gttgttcact aacgaacgag
     6961 ctaagatatg gctagattcc tgttctgcac tagctgcata cagaagtagc tcttcttttt
     7021 ttattccatt gactggctga atcgcctcta cttccaatat ccctttggta agcgttcctg
     7081 ttttatcaaa agcaatcgtt tttgtacgtg cgagcttttc aatcgttgtt ccagttttta
     7141 caacgatccc gtttttactt gaacggctca ttcctgcaac taaagcaatg ggcgcagcta
     7201 gaatcagtgg acaaggtgat gccactacca gtacctcagc aaatcgtaaa gggtcttttg
     7261 agaaccacca agaaatacca ccgatcaaat aagcaatcaa cgtgaaaggg actgcataac
     7321 gatcagctaa acggacaaat cgggcaggct tttcttttga ttcttcgact agtttaataa
     7381 tcatttgata ctggctgtct gcggcacgtt tttcagcgat catttggatc gatccgtctc
     7441 catttaaaga ccctgacata atctgatctc caggtttctt ggtttccggg cgggactcac
     7501 ctgtcaaaga agattcatcg accgaagatt ctcctttggt cactcgacca tctgcgggta
     7561 caagttcgcc aggcttgact agtaactggt catcaatatt tatttcatcg attactatat
     7621 cctgcaattt tccttctttg aatcgatggg caattcttgg ggtgttatct aacaaagact
     7681 gcagttcttt acccgctcgt ttattcgcgt aatcctctag actatctccg cccgtaagca
     7741 tgatcaatat catcaaacta gcccagtatt ctttgacggc taatgttgca aggattgcgg
     7801 taatcgctaa tacatctacg ccatatcttc ctgaacgtaa cgtcttgatc atttccaaaa
     7861 acatcaagaa ggcgagaatc cctccagtaa atgcgacaat cgcaaatgca agggccggct
     7921 ggtgaaaaac aaattcactt aataaagcta tcccaccaat aagcaaagta agtaaaaatt
     7981 ttttgaaatg tgtcattgtt ttttctcctc ttttatgtga cttagagagg ttagtaataa
     8041 atttccagct gcctctttca tcttaagcat agtccttgac tagcactttt taaaacaata
     8101 ggccttaatt gaaaacaact atcactatca gcgtttccaa aattcatcag ttctacaaac
     8161 aaaaatattc agcaaggatt actttttcga ttcatcagtt aaaaagtacc ttttttcgaa
     8221 aaactatgct atacttttaa ttcgttattt acggcggaag gagcaaacat gcgatgaatg
     8281 agaaacaact agaacaaacg cacgtggatg aaacgatcga actaatccag atcgaacaaa
     8341 atatgctgga tacaaaacaa caagaattga ctcaggaaat gaatcattcg acaaaagaca
     8401 cagctaatca caagattcga gctggttcca atgaagcttt ttatgaatct gctgttgaat
     8461 atcgccaaca tgagcaggaa ttgcttttga aataccatac tcttgaagca cagcaaaaac
     8521 gcctgcaaac gttgtctgtc atgaaagata gtccttattt tgcccgtatc gactttaaag
     8581 aagaccgtga aacagaaaca ttatatctag gtatcgcttc tttaagagat ctggcagaag
     8641 aaacaatcgt tattgactgg cgcgcaccaa ttgccaatct ttattatgaa ggtgagattg
     8701 gtcccgcatt ttatgaaact gaaacagata gaatagatgt cgatctgcag ttgaaacgac
     8761 aatttaaaat ccaagaagga cgtattttat ctatggtaga tacgtcggaa gtaatcaatg
     8821 atgacttttt gctagagata ttggatgaat catcaagcaa ccatatgaaa aacatcgtct
     8881 ctacgataca aaaagcacaa aatgcaatca ttcgggacac aacaagtaaa gtaatgctga
     8941 ttgaaggaat cgccggaagt ggaaagacat ccgctttgct tcaacgtatt gcttttctgt
     9001 tgtatcataa tcgcaagtgg ctcgaggctg aaaacgttct tctattttca cccaaccatt
     9061 tgttttctga ttacatctcg actgtattgc cttcattagg tgaaagcggc gtgcctacac
     9121 agacattcaa aaattatttg gtgcagctgc tgccagagtt ttctttacta gatgaacagc
     9181 aacaagaaag tggttttttg ctaggggaaa aagatccgat acaagtgatg aaaagcggat
     9241 tgaccttagt cgatcagatt gatcgctata tccaaggcat cacaaactac ggtcccttgt
     9301 tccgtgatat gaaaataaat ggccgtacga tcctatcaaa ggaatcgatt cgccaatggt
     9361 ataaagaaac aaatgaacaa ttacctttac atcatagact ttctctatta caaacgaagc
     9421 tcctgaaaaa attaggcggt ctgcaaaaag acgaaacacg tcagcaatgg gtcaaagatc
     9481 tggcggaaga acagctccaa gaactatacg caacagatcc aaatcttgaa tatacagaac
     9541 aaaaggagcg ttcattacgt aaaaaactgg ccaaccaaat cgtacgtaaa agattccgtc
     9601 gaatccatcg agggatcatg aattatcaat ttgtcaatat aacgaaacaa tatctccact
     9661 ttctacaaac tgtgccactt tctcttctaa cacgacataa aatcagcgag accaggtggc
     9721 aggaacattt gacaactgtt cgtacacact tgcgtcaaag agaattgcgt caagaggatg
     9781 ctgtactatt ctttttactg atgcgccgaa catatccagt gacggttaca cacaaagctc
     9841 gctatatctt tattgatgaa atgcaggatt tcgcaccatc acaagtggca ttgctgcaag
     9901 ccttgttccc aaaagcaaat ttgactttct gtggagatct aaatcaaaat gtctttggaa
     9961 atgaaacgat cgttggatca ttggaagacc ttttcgtgga ccgtgaagtc actcgtttcc
    10021 aattaacgac aagctatcgc tccacaaaag aaatcactga ttttgctaat cattttattg
    10081 tgaaagaaca tcaagtagaa acaacagcaa gaaaaggaga ccttcctttg ctcatccaag
    10141 gaacaacaga tgatatcaaa ttgaattggt tagcagatac cattcgaacg actcagggaa
    10201 aagcccggta ttggcgtaca gcaatcatag ctaaaacaca agaagaatgc gaagctcttt
    10261 atgctcaact gccagacgag ttgaaacaac aagtccagct gattttaaat gagagtgact
    10321 ttatgaagcg ttccattatt ttacttccag cctttttagc aaaaggtctg gagtttgacc
    10381 gagttttctt gtgggatgta gatgcagaac atttttcttc tcagcaagac aaattgatcc
    10441 tttacaccat gtgtacacgt gcgatgcatg aattgaccct ttttacctcc aataatgaaa
    10501 gtcctttgtt aaacaatatg gatatttcat cttatgagaa aaaagtactg taagtaaaaa
    10561 gacaactgct atcattaatg ttagcagttg tctttttatt tacaataata attgcagaaa
    10621 aagtaattca atcatttaag gtcatttatt ctatatataa atccaaatct gtttcaggat
    10681 attttaattt gacaagcgaa ataatcattt tcagttcttc catgttttct ggatattctg
    10741 ggtcaaatac cttgctatga cggccaatcc cgccattgtc gatcaatcgg taaattcgat
    10801 tccattcaat gaattggtca attgattgga aattgctgat tggatagccg ccacaatcca
    10861 aaatctctga tacacgaaat gctaggtatg cactatcgat cactttattt tttttcgtgt
    10921 ctatcttctc atttctttcg ataagaattt tttcaggacg atgtttcaag ctccactcta
    10981 atagtagaaa gaaagcctcc atccgatact cttcttcaaa taaccgaaag gctgaatcag
    11041 aacgacgaaa aagcagcgga atcagataat tttgtctata aatcagcatt ggtgcgcggt
    11101 ccaccataca ctctaaataa cggttcagat accctgtacc gacatagttt cctaatcgat
    11161 agccttcaac gccgaatctt gcgtattttt gcgctaattt tgaacaatca gttacgttca
    11221 cagctaactc ttctactttg cggtaaccgc ttaagatacg ttcaaaaggt tctaaagtga
    11281 tttttgcttc catattcttc cccgtccatc ttccacatac ttcatatcat ttcacatgat
    11341 acttatgctc tcatcatacc acaggttgaa ttagaaaaga aaataaatca cacaaaaaag
    11401 caagacaatc tgttagtgag attgtcttgc ttttcgaaaa agactgaata cttttcgtta
    11461 aaactggttg cttagtcttc taatgccaca ttatggaaca catgctgtac atcttctaga
    11521 tcttccagtg catcaatcat tttttcaaat gtttcttgat cttctggaga tagtgttacc
    11581 tcgttttgag ggatcatttg gatctcagcg attgaaaaat cagtgattcc tttttctttt
    11641 aatgcttctt gtatactatg gaaatccgct acttcaccgt aaacgatgat ttgtccttct
    11701 tcttccatta catcgcgaac atcgatgtct ttttccatta ggtattcaag gatctcgtca
    11761 gcgtcttctc ctgcaaaagc aaagattgct gtattgtcaa acatgtaaga aaccgctccg
    11821 gaaacaccca tgttcccgcc atttttccca aatgctgccc gtacgtctgc tgctgttcga
    11881 ttcacattgt tcgtcaatgt atcaacaatc accattgagc cgcttggtcc aaatccttca
    11941 tagcgcaatt cagagtatgt ttcatctcct gcaccttttg ctttttcgat tgctcggtca
    12001 ataatatgtt ttggtacatt ataagtttta gcgcgatcaa tgacgaaacg tagtttttgg
    12061 tttgcatgcg gatcaggatc acctgatttt gctgccgcgt agatttcaat cccaaatttt
    12121 gcataaatcc ggctattatt agcatcttta gcagttttct ttgctacgat atttgcccat
    12181 ttacgtccca tattttcact tcgctttctg atttgatagt ttcataacgc aaaaacgtcc
    12241 tgttccatta tacttgtgaa tcacgattct gtgaagcttt ttttctttct tccttcattc
    12301 ttctcatttt tagtcaatta tctatactcg gattgggatt tgcccgaaat tctgttccgg
    12361 aatattgcct cccaattgat agatttgatc acttctaact aacagttccg cttcttgtgc
    12421 ctcttttttt ccgattcggg aataaataga aatctctttt ttttctttta agaatcttct
    12481 tccagcatcg gtaaatccaa gtagtcgaag ataattttta ttttgttgga caggaatctc
    12541 ttgctttttg atattcagca agacataaca agataatcgt tggatccgtg tccatgtata
    12601 tctttttgtt tttacctttg acacaaaatc ctcaaaacta tcagcagttg cagctgctgt
    12661 tttcatgcgc gattctattc cttcggtcat ctgatagata tctgtcagtt ccagcaaagt
    12721 agaagataac aaccgataac gcaaatacgg ccaataagat tcccaagtct gcagcgaaca
    12781 agttgaaaga tcttcttctg tctgaggcgg cacaagtcca gctattcttt ttccttcttc
    12841 taacgcatgg cgaatggctg ttgcactggc tatcctctga tctgtgatga ttttttcatg
    12901 atagcctgca cttttacgtc ggatcggatg taattccata gggtggacat acttggcatt
    12961 ttcctttgca taacttaatc ccaagatatg atttggcgaa gaaaaatcaa gcgtaatatc
    13021 gggatacaga ctgcgaaaaa cctcagtcat tttttgcgga taactatacg tttgatcaga
    13081 cagttcttga aatgcctctt ctatcctatc ttgattctct tggacaaagc gtccgaaact
    13141 gctgtaatca aacgaagtct cttcttctgt tccaaaacac aaactgtcac agtccagtgt
    13201 ttgcaagatt tttactgcac ctttagcaaa atagtcagct gactgaaccg cccattcgac
    13261 gggaagctcc actactaaat ccacaccatt ttgtaaagct tcattcgcgc gatgccattt
    13321 atcgatgata gccggttcgc ctctttgcaa aaaattcccg ctcattacag caatgacgac
    13381 ctctgctcct gttatttttc gtgccatttc cacatgatag cgatgtccat tgtgaaaagg
    13441 attgtactct acgataatcc cgcagctttt catgcgcatc ctccttcata gttatcgata
    13501 aaaaagaaat agagacaagt aaaaaaaggc aaaaatcgga aaaaataccc gaactatata
    13561 aactattctc gcaaacacaa tacttctgcg acgaaaagct gcactgcaag agcaatgcct
    13621 atatcgttcg gatatataat ttatgttgaa gatttattct tttcttgtta actcgagcag
    13681 gcaaaaccgc tagtctttgt atgctacaaa gaaccagcgc tttgatttct ctgatgctgc
    13741 ttcgtctgta aaatcagaaa atgcttcgac tttcgaaaag ccgctgtttt ccagcattct
    13801 taaataactt tccaatggat aggtacgctc ttgatgtagt tcatcttcac gaataaatcg
    13861 accagaccct tcttgttctt caacgaaaaa ggtaagaaaa tgttcgatgc tgtgtttctc
    13921 gcttcctgga taactatccc acaaaaaagc aaaattatcc ccttgataat gatagctata
    13981 ttctggaaaa acctcatcta cttgataggt ggagtgtacg tcaaataaaa atcggccatt
    14041 ttcttctaac gcttgataga cttcatcaaa cacttgctgt acctcttgtc tatttttcat
    14101 atagcacagc gaatcagaaa aacaggtaat tgcctgatat tgtccgattt ctgataaatc
    14161 gagcatattg ccttccacaa attggatcga tgcgtcttct tcatatgctc gctggctggc
    14221 aatcatcaac atctcttctg aaaggtctaa ccctgtcact ttatatccat cttttgcaaa
    14281 ggcaaccgct aatgctccag taccgcatgc cagttccaaa atattttttg tgtcttcttg
    14341 taaatgacgt ttcgaaaaag ctaaccattg ctgataaagt gtttcgtcca tcacttcatc
    14401 gtaaatgaaa gcaaatgttt cataagccat atgttaatcc aaccattcac taagatcgac
    14461 tagcggagca tctgaccata gtttttccag attatagaaa tttcgttctg gcgcatggaa
    14521 aacatgtacg atcacatctc caagatcgat caatacccat tttccgcctt cttttccttc
    14581 aatacgtttt acttcatatt tgttttcttc ttctttatca atgatttctt ctgtaattgc
    14641 gttgatttga cgttcgctat ttgctgaaca gatcatgaaa taatctgcca acaaggaaac
    14701 atttcgaacg tctaaagcga taatgtcttc tgcccgtttt gaatctgctg cttcaatcgc
    14761 aatttttaat tgttctttac tatctatgat gatttcctcc taatatgagc agcttatttt
    14821 tttgctaccc agtaattata tgtttcgatt gtttttggat aaatctgttg ttcttgcgca
    14881 agcaaatggg ccaatgtatg ttttgtttca aatgctacag cttcgtctag atcagcccat
    14941 gcaatggctc gtgcatcttg taccccagga aaatctcgac ctggttcaat atagtctgct
    15001 acataaatga ttttgtcaag gagactcatc ttagcagccc cagtcgtatg cacccggatt
    15061 gcatgaagaa tctctgcatc ggtgattcct aattcccgct ctacaaacga agcgccaaca
    15121 agaccatgcc agattgcatt attataattc aataactccg gatcaaaacc gtctcttacg
    15181 attaccattt tgaactcctc atctggtcgt tcttttgcat aatcatgtgt caatgcagca
    15241 atactagctt tttcaggaga agcaccgtat tttttggcaa gagccacggc tgtttcttct
    15301 actccaagca catgcttgaa gcgttgttcg ctcattctca tctgtatttt ttgcatcagg
    15361 ttttctctag aataagcagt atattcatta ctatagtcca tattcataca acccttcatt
    15421 ttgaatataa tcaattactt tatctggaac aaggtagcgg atagaacagc cttctttgat
    15481 cttttgacgt attttcgtgg aactgatgtc gatctcaggt acatctaccc aaattacagg
    15541 atatggagta tccgttgtgt atcctggtcg acgtataccc acaaagttga ccatagaagt
    15601 cagctcatcg attttatacc attttggcaa atattctacc atatccccac cgataataaa
    15661 gtaataatct gtatccggat tgttttgcgt caattccttc atagtatcat aggtgtaact
    15721 tttcccacca cgagctaatt caatcgtttc aatctgcaaa aaaggattgt cttccaccgc
    15781 taattccaac atgttgagtc gatgttttgc atcaatcgtc tgtttctcat caacatgagg
    15841 tggctgataa gaaggcatca aaaatacgcg gtcaagacct aaattccggc cagcttgttc
    15901 agccatcact aaatgtgcaa ggtgaacggg attgaatgtt ccgcccaaga tccctacttg
    15961 ctttttcttc tctaagaatt gaggcatttc ttcaggaaat aattgtgaac gaacaaatgt
    16021 cttagcttgc gtattcatca tggtcactcc ttatattgct tttacttctt ttgatagcct
    16081 ttgatacttt tcttttgtgg aaggtttgta taatacaata acacgtccaa tgatttggac
    16141 gatttcgcaa tctatttctt tttctaatac ttgagctgct tcttctgcaa tctcatctgt
    16201 attttgaagt aacgatactt tgatcagttc tcttttttct aaagcttctt caatttgaac
    16261 gatcattgct gaattgatcc caccttttcc gatttgaaaa atcggctgaa gatgatgcgc
    16321 tttgctacgt aaaaaacgct tttgttttcc tcttaaactc ataattttcc ttcttcctta
    16381 aatcaatgct ttgcgtttca atacatctac accctttggc gcccagcccg caattactac
    16441 tggcgctgtt acagtgatcc agcctagacc ggcaaaaacg atgtctgttt tttctttcac
    16501 agaaaactca aaacgaacaa gctccggaaa tgcctcgact tcatcaggac gaggaggctg
    16561 taataatcca cctacatgtt tttgataaaa ttcgtctgct ttttccagtt tcgttcgatg
    16621 aatatttaca tcatttgaca cataagcaat aaatgaagca cgattgccgc tgataaaatc
    16681 aaatcttgca agtccgccca agaaaagtgt ttgcccttca ttcagctgat aaactttagg
    16741 tttgatttct tttaacggtg caatgatccg caaatctttt ttacctaagt agtgcgccat
    16801 ttgatggcga tggataattc ccggtgtatc aatcaaaaaa tgcccgtcat ctaaaggaat
    16861 ctcgatttta tccaaggtcg ttcctggaaa ctgcgaagtt gtgattacat cttgtactcc
    16921 cgctgtattt ttgatgattt gattgataag cgtcgatttt ccgacatttg ttactccgac
    16981 gatatagaca tcccgtccat cgcggtattc ttcgattttt tctaatagtt ccgtcatctc
    17041 atgagatttc ttagcactag ttaataatac atctactgga cgaattcctg cttcgtaagc
    17101 acgttccttc atccattgtg ttaatttagt ttttttcaat gatttaggta atatatccac
    17161 tttgttccca actaaaagta ctggattgtc accgacaaaa cggtgtagtc ctgggattag
    17221 tgaaccattg aagtcaaaaa tatccaccac attgacgatc aatgcatctt tttgccctaa
    17281 ttcattcagt aagcgcaaaa agtcgtcatc cgtcaatgag acatcttggg tctcattata
    17341 atggcgtaat ctgaaacaac gctgacaata gacttccccg gaagctaaac ctttttccaa
    17401 agccgtttgc ggcgtgtatc ctaattcacc gggtttgttt gtttgtataa cggcaccaca
    17461 gccgatacat tgtaattctt ctgtcattcg attccacctc gccagcccat atcagggtgt
    17521 tttgctaata gataggccat aattcttttt tcccaaaaac gattaaattg tgtcttccaa
    17581 ctatctgttg cgataatggg ttttacaagg atgctacgta tccctgcacg attggctcca
    17641 cgtatatcag tcataatctg atcgcctacc attactatct catcatctgc taaatccaat
    17701 tgtttctttg ctatgttgat ccctcttgct aaaggtttca ttgctcgtgc aacatacaaa
    17761 agatcaaatt tttcaatcgc tcgcgcgaca cgttttgaat tattgtttga aacaacaaca
    17821 acagggattc ctgcattttt catttctaaa atccaagtaa gcaactcttc tgttccatct
    17881 ggattattcc atgcaatcaa cgtattgtct aaatcggtca atactgcttt gatgcctaga
    17941 ttctttaatt gggcaggtgt aatctgatag atggcttcta ccatccatgt tggtttgtaa
    18001 tttgaaaaca tgctgtctcc tcatctgcaa aaaatgaagg agcacataaa gtactcctca
    18061 accccgtatt atactgcata tcagcaaaaa aaactacctc tatactcgtt cgtctttcag
    18121 aaaaagcatt tctttacaaa atgaccaaaa ataaagattg atcacagaaa aatttagaaa
    18181 caaaaaacaa ctaatattgg cggaacctct tgtatcgttt ttccagtaat ttatgctgat
    18241 ctaatcgaac aagttccgtc aatctttcaa tcaacgcttt tttcataatg tgtgcgactt
    18301 tatatcgtgg caactgctcg ccgttgaacg tttcaggaat aactcgatca acgattccta
    18361 actcgcgtag ttctggcgca gtaattttca tcaactcagc tgcttctgcg gcgcgactac
    18421 cgtctttcca caaaatcgac gcaaatcctt ctggagaaag cacagcataa atcgtttctt
    18481 ccagcatcca gacttcatcc gctaaaccta gagcaagggc accgccactt ccaccttcac
    18541 caatgatgat ggcgatgatc ggtaccttta aatcagacat ttcgagcaga tttctggcaa
    18601 tagcctctcc ttcgccgcgc tcttctgctc ctacaccgca atatgcacct gctgtattga
    18661 tcaaggtaat caccggtcta ttgaactttt ccgcttgttt catcagtcgt aaagcttttc
    18721 gatagccttc aggatgggga gaaccaaaat ttctgcggat attttcctgc agatcccgtc
    18781 ctttttgaat accgatcact gttaccggtt tgccatctaa tcgagcgatt ccaccaacaa
    18841 tcgcttcgtc atctgcaaat aaacgatcac catggcattc ctcgaaagaa tcgaagatct
    18901 cttcaaaaaa atccaagctt gtccatcgtt ctttagctct tgctaactga acgacatcat
    18961 gtgctgtctt atccacgatc tatccatcct ttctgtgtat gcaattcaag aagcgtatgg
    19021 attttctgtt tcaattccat gcggggaaca atctgatcga caaaaccatg agataataag
    19081 aattcagcct tttggaaatc ttctggaagt tcctgcttga tcgtctgttc gatgacgcgt
    19141 cttccagcaa aaccgatcaa gctttggggt tctgctagaa taatatcgcc ttccattgca
    19201 aaactggcag ttactcctcc ggtcgtcggg tccgttaaaa cagttagata aaaaagacct
    19261 tcattgctat gccgtttgac agcagcagag atttttgcca tctgcattag agaaaagatt
    19321 ccttcttgca tccgtgcgcc acctgaagct gtgaacaaga cgactggaag actttccttt
    19381 gttgctcgtt cgaataatct ggtgatcttt tctccgacaa tcgttcccat acttcccatg
    19441 ataaaattag gatccatcac tccaataatg aagggaatcc cctgaatggt tgctttacca
    19501 gtgaggactg cttcatgaag tcctgtttta tcttgcattt ttttaatttt ttccgtatat
    19561 tcgggaaatg ctagcggatc tttggttaca agatccgtat cccattcttc aaaacttttt
    19621 tcatcaataa tcaaagctag acgctgccat gcaccgattc gaaaagcgta tccgcaatga
    19681 gggcatactt tttcttcacc gatgtctttc gtataaagga tatgtttgca atttggacac
    19741 tttgcccaca tgttatcagg aacagatggc gagttagcag gtttgctctc tctattagga
    19801 ttgatccgaa tatatttctt tttcttaaac agagccatct atgcgcctcc tatctccgtt
    19861 tctggtgtcc aattcggtaa aaattcttct tgtaaaaatg ctgtgctgta atcacctgct
    19921 atgactgcag gatgactaat caaatccatc tgaaattctg cgttcgtgat gattccttct
    19981 gtgataaatt cacttaaggc tcgctgcatc ttcattaatg cttcaaaacg cgtatttcca
    20041 tggacgatta tttttgcaat cattgaatca taatatggcg ggatcgtata tcccgggtaa
    20101 actgcactgt ctactcgcag tcccatccca cctgctggaa gcagtaagtt ttggatcttc
    20161 cctggagaag gagcaaaatg gaaagcagga ttttctgcat taatccggca ttcgattgcg
    20221 tgtccttgga atactacttc ttcttgacac acattcaaag gctcaccggc tgctatctcc
    20281 acctgttttt ttactaaatc gattcctgtg accatttctg ttactggatg ttccacttgg
    20341 attcgagtat tcatttccat gaaataaaaa tcgccttctt catccatcag aaactcgatc
    20401 gttccggcat tttcataatt gactgcttta gctgctcgaa cagcagcgtc tcctagtgct
    20461 tctctttttg tttgtgagat cactacagaa ggagattctt ccagtacttt ttgattgttg
    20521 cgttgtaaag aacaatctcg ctcgcctaaa tgaatgacat gaccaaatcg atcacctaag
    20581 atctgaactt ctatatggcg agcaggataa atgatttttt ccaaatacat atcatcattt
    20641 ccaaatgccg ctgctgcttc ttgctgagct gaagaaaaat gctgtgggag ctcttctttt
    20701 gattgaactt tccgaatgcc tttcccgcca ccacctgcag ctgctttcag catcactgga
    20761 taaccaattc tgtctgctat ttccaatgct tcttctactg tagataatac accagtactt
    20821 cctggaatga ctgggacatt tgcttcttgc atcaattgac gcgcattgat cttatttccc
    20881 attgcatcga tcgttgcact tttaggacca ataaatgtga tattgcactc ttcacacatg
    20941 gaggcaaatc ggctattttc agataaaaaa ccaaatcctg gatgaattgc ttcggctttt
    21001 gtcacgattg cagcgctcag tacttcctga acattcagat atgaatcaac ggcttttgcc
    21061 gggccaatgc agattgcttc atctgctaac tgtgtatgaa gtgcttcggc atctgcttct
    21121 gaatatatcg ctacggtttg gatccctagt tcgcggcatg cacgaatgat acgtacagcg
    21181 atttcaccgc ggttagctat caatacttta gaaaacatat ttcacctatc caatcataaa
    21241 ggttaattcc gcttcggcta cttttttacc gtctacttta gcgattcctt tgcctattcc
    21301 ggctgaagct ttcactttca atatctcaac ttcaaggtat aaggtatctc ccggtgtaac
    21361 tttcttacgg aattttgctt tgtccaatcc gccgaaataa gccgtctttc ccttgaattg
    21421 ttcaagagaa agtaatgcaa ctgctcctgc ttgagccatt gcttcaacaa tcaatacccc
    21481 tggcataaca ggttcttctg gaaaatgtcc ttgaaaaaag ggctcattga tcgttacatt
    21541 tttcttagca acgatccgtt ctccttcaac catttcttcc actcgatcaa tcaaaagcat
    21601 tggataacga tgtggaatga tttcttttat ttcttgtatg ttcattgttt tccctctttc
    21661 ctgctttgct tatttttgct gaatcctaaa gagtggctgt ccatattcga caactgactc
    21721 gttttctaca aggatttctg tgatcactcc gtcggcctca ctagttatct cattcatcag
    21781 tttcatcgct tcaataatgc agataacttc tcctttggca acttgatcac ctactttttt
    21841 gaaagctggt ttgtcgggcg ctggttgcag ataaacgatt cccacgattg gcgaaacaac
    21901 ctcgattcca tcaaatactg ccgattcttt ttctttcatt atttctggag cagttgttgc
    21961 ttgttcaggt atgacattga ctggctccga aactggcaac gtttcgtttg tttgaaccat
    22021 attcatgcct cgacttgttt tatttttatt catatacaat tcaaattgac cttcacgcaa
    22081 atcgaattcc gttagcgagg attgatcaaa ttgactgact aattctctga tttcattgat
    22141 atccaaaatt attcctccca gcgtttcatg cacagaaccg cattatgtcc gccaaaccct
    22201 aatgaattac tcattgcata agtaaaactt gcaggtttgc tttcgttttt gacaactgtc
    22261 aagtcaatct caggatcttg tttttctaca ttgatcgtag gtgggataaa ttgatgttct
    22321 aatgccagca aagttgccaa ggcttcgatc ccgccggctg ctcccaataa atgtccagtc
    22381 atcgatttcg tgctgctgac gtaagtatct ctgaaatttt cacctaatgc gatttggatt
    22441 gcttttgctt ctgcctggtc attggctggt gtacttgttc cgtgagcatt gatatatccg
    22501 acttgttccg gctgtacacc agcttcttcc atcgcaagct tcattgcttt agatgcacca
    22561 gaaccatctg gagtaggagc tgtcatatgg taagcgtcac agtttgcacc gtatccaacg
    22621 atttctccat agattttggc accgcgagct tgtgcatgtt ctaaagattc aagaactaga
    22681 acgcctgctc cttcacccat cacaaaacca ttccgatttt cgtcaaacgg aatcgaacca
    22741 cgattaggat cagttgattc tgagagtgct gttaaagctg caaatccagc aatccccatc
    22801 tcacaaatcg ttgcttctgt tcctccagca agaatgacat cagaatagcc atgtttgatg
    22861 ttacggaatg cttccccaat tgaattcgtt ccgctagcac aagctgttac gatagctgta
    22921 caaatacctt ttgcacctac acgcaaagca atatttccag ctgccatgtt ggcaatcgcc
    22981 attggtacga acattggaga aactcgtttt gcaccttttt catgtagacg aataacttgg
    23041 ttttcgattg ttggaagacc accgatccca gatccgatga tgacgccaaa ccggtcttga
    23101 ttcatttttt ctttatctaa accgctcatc tccattgctt ctaaagcagc gtagattcca
    23161 tagatcgaaa acatatccat tcgtttgcta tcttttttaa taaaatattt atcgtaaggg
    23221 aaatctttga cttcacccgc taaggtaatc cctgtttctg aagcatcaaa tttagtgatc
    23281 ggtgcgatgc cattcgtccc ctcttttaag ctattcaaaa agctgtctgc gtcatttcca
    23341 ataggtgaag tgactccata gccagtaatt accactcgat tcatttgttt gctctccttt
    23401 tcttatccgt gcatgaccaa tccgccatcg acattgatga cttgtccggt gatatatgga
    23461 ttttgtgcta agaaaactgc tgtttgtgca atatcttcta cttgtccgaa acgctgcatc
    23521 ggaatttgct tttccgcttg ttccttgact ttgtctgcta aaacttccgt catatcagtt
    23581 gtgatgaatc ctggcgcaat ggcattacat gtaatgcctc tagttgcagc ttctcgtgcc
    23641 acagattttg ttaatcctac tacgccagct ttactagctg cataatttgc ttgaccaata
    23701 tttccaatca acccagatac actagagaga ttaatgatgg ccccttctcg ctgcttcatc
    23761 atttttttca atacgtgctg cgtcatattg aaggttccaa tcaaattgat atccagacat
    23821 tttttgaaat cttctgcatc cattcgcatc acaagtttgt catttgtgat tccggcattg
    23881 tttactaaca catggatcga tccaagcttt tcttctgctt ctttgatcat ttgtcccgct
    23941 ttctcataat cagagatgtc accggaaact cctacgcatt ttacaccgaa tgcttcgatt
    24001 tcttctatct tttcttttgg aatctctccg cgaccattca aaatgatatt tgctccagct
    24061 tttgcaaaag ctaaggccat cgctttgccg attcctcttg tactacctgt gataaataca
    24121 ttttttcctt ttacatccat aacgttcctc cttatagtat tgctattgtt tcatcaaatg
    24181 tctgcttatt ctcaacacgc agaactggta ttgttttatc gatttttttc ataaatccgc
    24241 ttaatacttt tccggggccg acttcgatga cttgttcgat gccgattgtt tttaacttat
    24301 ggatactctc atagaaacga acaggttgca tgacttgctg ttcaagtagt cccgcgattg
    24361 tttctttctt cattatttcc gttgttgtgt tgcttatgat aggaaaagaa ggttcagaga
    24421 attgaatctg acttaggtcc ttcgccagtt tttttgctgc cggttctaaa atagctgtat
    24481 gaaaaggccc gctgacattt agagggatca ttcttttgaa ccctttttct tttaatagta
    24541 aaacagcttc gtctactgct ttttcttcgc caccaatcac gatctgctga ggtgtattat
    24601 aatttgcagg agatacgatg ccgtacttgc tagcttcatg acagctttcc tcgatcgttt
    24661 caatcggcgc attcatcact gcaaccattt tcccgcttcc agctggggca gcttctgtca
    24721 tataagcgcc tctttttgct actaacgcaa ctgcttcagt gaaggatagc gcattactag
    24781 ctactaatgc cgaatattct cctaaactca gtcctaaagc tgcatctggg atgattccat
    24841 gctccttcaa taagcgataa tacgcgatgc tgactgttag aatagctggc tgtgtatatt
    24901 gcgtctgatc caaacgttca ttttctgtaa agcaaagttc agccatatcg tagccgagaa
    24961 tctcacttgc ttcatcaaat gtctgcttta caacggcttc ttcatataaa tcttttccca
    25021 taccttgata ttgtgcacct tggccactaa ataagaatgc tgttttcatg tttttctcca
    25081 atatatccag aatatatgtt tgattcttcc tattcaaaaa ggccgagaca ttcctatttc
    25141 aggaaatgtc ccagccccca acctaatttt aatgattatg cagtttgttt ttctacgtaa
    25201 gtcactaaat cttgaaccgt tttgattccg tcttctgttt cgattttcac gtcaaattca
    25261 tcttcaattt cattgatgat ttggaataaa tccaagctgt cagcttctaa gtcttcttgg
    25321 atgtcagtag tcaatttgat ttcttcagtt tcttttccta attcttctgc gatgatttct
    25381 tgaatttttt cgaataccat aataatatat tcctccgata ttgtttgctt tttattttta
    25441 taatttaaca gtctacaact ccatgagaat acttccccat gttaaacctc cgccatagcc
    25501 tgtaaatatt actttctgct gttttcctaa ggtgatcttt ccactagaaa ctgcttcatc
    25561 caataagata gggatgcttg cagcagatgt attcccatat ttatccatat tggtcaggaa
    25621 cttttcttgc ggcatcttta cttttcgggc aattttctca ataattctga ggtttgcctg
    25681 atgcaaaagc agataatcaa ctggtttgtc tttcatgact tcttggatat tgtttgcaac
    25741 gtctcgtacg gcaaaatcaa aaatatctct gccagtcatt tgcaaataat acgaaccttc
    25801 tgaatcttct gaatagaacg gatttttatt ttgctggtag ccagcggtca atgagctact
    25861 tctggtaccg tcagctgcta atttttcttt cagcaatttt ttctgtggat tcgcttctaa
    25921 gagaatccca cctgctccat cgccaaacaa cacagcagtc gtacgatcag accaatcgat
    25981 tagcttagat agtgtttcac caccaatcac cagtccctta gttgagccgc agcggatcat
    26041 tttttcagcc atgcttaacg cataaacaaa tcctgagcac gcagcactga tatcaaaagc
    26101 gatagcatga gttgctccca atttcccttg tacttgtgct gctacagatg gtgtcgtgta
    26161 atcgggcgac attgttgcta ctaaaataaa atccaaatct ttagggttta gatttttctg
    26221 ttttatcaat ttttccgcca ctaaaatgca taaatcagaa gtctcctgat ttgttacaca
    26281 tcttcgttca cgaatacccg tccttgacga aatccattca tcagaggtat ccatcatctt
    26341 ggaaagatca ttattagtaa taatcttttc aggaaggacg ctggctgttg cagtaatcat
    26401 tccaaattgt tccattaaat atcctcactt atagtcttgc aagaagtcgt gcaagttttt
    26461 cagtgcatcc agcaaggcat gctgttcctc cgtagccatt ccgtctaaga tgttcttgac
    26521 catttcacga tgaaaatgct gatggacccg aaatagtaac tttccttttt tcgttagtcc
    26581 caactttaca acgcggcggt catcttcact gcggatacgt tcgacatagc ctttttttac
    26641 taacttgtta atagcagtgg tcaaggtccc aacagtaatt gatagttctt tggctacttc
    26701 tgaagttgtt tttttcttgt acatcccgat tgcctcgatc gtgtgcatct ctgtgattga
    26761 cagatctttg aattgagatt ttttcagctc agactcttcg attgtcaaaa tatcattgaa
    26821 gacactaaca agataatcat ttactgtttt taaatctggt tccatgttcc accgtccact
    26881 aattagtttg attatcaaat cttttgatgt tcaaactata tcttaacttt tttcaaaatg
    26941 caaccttaaa agatcatttt tttactatat gagctttttt taagatttta ctcatttaaa
    27001 acaactgata aatcagcatt tataatttct tttattagaa aaatcatcta tctcacatat
    27061 tttgattatc aaagtatttt ctgtgaacag aactatagaa ttccctaaaa cttgtccatt
    27121 atttcaaaat ttgttatagt ctttttattc aaagcgtaaa aggagatcct atgttaaaag
    27181 aaaaaatgaa aactctcccc atgctgccgg gtgtctatct tatgaaagac cgagatggta
    27241 aggtcatcta tgttggtaaa gcaaaacggc tcaaaaatag agtaagcagc tattttcatc
    27301 agaataaaca gcattcaaaa aaagtattac gtatgattca tcatataaca gattttgatt
    27361 tcgtcgtagt agacacggaa ttagatgctt tattgctcga gtgtcaattg atccaacatt
    27421 accgtccttt ttataaccga caaatgaatt atttcagtaa ttataattat gtacatatta
    27481 ccaataaggg atttgtatta acagatactc ctacggctcg aacatacggg ccttttcgtc
    27541 tctataaaaa aatgccgtct attttgcgta taatggaaga aacttatcaa atgccttggt
    27601 tgtctgaaat tagtttgtta gctttgcgag tccagcttcc tgacctacag gaaatgactt
    27661 ttgaacagaa aaaaaaggaa ctccaagggc tgtttcaagg ccgaaataaa aagctactga
    27721 cttacttgaa aaaaagacaa caacatttca tctatcagtt gaatttcgaa aaagccggta
    27781 tgcttcaaaa agatatcgaa ttagttactt attttattag acggatccag gaacaaaagc
    27841 agtttttaag aacaccatcg ctcaccttca gcatgccatt agcagcagat gagagtcaga
    27901 aaaagcacta cctcgtttgt tatggtcaac ttgcagaaac aatgattacc tctggtgacg
    27961 tttctcctga cttctattat gaaaagaaag aagctcattt atctttgaaa cgtcaattgt
    28021 caaaagaaga aatcgatcct gtacaaatct tgatcagtta ccgcaaaaaa ttagaaaaag
    28081 aacagtttga aattgaactg ttaaacaaaa aagaagctga aaagcaacta aattagttgc
    28141 ttctcggctt cttttaggca tcttcaactt caaatgtcaa ttatcctaaa atttattgga
    28201 ttaagtcata gatttccatc gctacgatat cgatattatc aaattcgtat tgctgatgag
    28261 tatctgtttc agttaattca aatgtatcgg tcgctttatc ataagttact gtacacttct
    28321 ctacgccttc tttttcaaaa cggcgaactt gcagttcatt atctgctgac tcgaccatag
    28381 cctccagtcg ttttatgatt gcgactaatt gtgaatgtgt catcggaggt cctccttttc
    28441 ttatttctgt tatattgtat caaaagcata gcctacttgc attgattttc tctaaaaaaa
    28501 ctcgtttttt atgatttttt ttctggtatt tttccttcag gccaccaaag tttgatcaaa
    28561 gcttgggttc cgtcattctc aaaaccttta tctgccaact cttcatatag tttcaatgct
    28621 tgggtagttg ctggcaatac aagatccatt ttttctgctt cttctaatgc aatctttaaa
    28681 tctttgataa aatgtttgac aaaaaaacca ggtgtgtaat cttcttttaa aatacgagga
    28741 gaataattgc ttaaagacca gttagctgca cttccacctg aaagcgtctc aatcactttc
    28801 tttaaatcaa gtccgttttt ttgtgcatag acaagcattt ccgtcattcc tgtcatcgta
    28861 cctgcaatca tgatctgatt agccattttt gtatgctgac ctttcccagc agaaccatgc
    28921 aatgtaaatg tcgtaccaaa ttctttgaat aaaggcagta cttgatcata aacggcttct
    28981 tctccaccga ccatgatcgt cagtgtacca ttctttgctc ctaagtcacc accggatacc
    29041 ggtgcatcta acgcatctgc gcctttttct tttgctgttt ttgcaatttt ttctgccagg
    29101 cttggtgtac ttgtagtcaa atcaactaaa atatgtcctg agatatctgc agaaaaaatc
    29161 cccgactgtc catagtagat ttcttctaca tctttggggt aacctaccat tgtaaagaga
    29221 atattacttt gttctgctac cgcttttggt gtatctgccc agatagctcc ttctgttact
    29281 aaaggatcag cttttgcttt tgtccgatta tagacagcta cttcgtgtcc tgcttctagt
    29341 agatggcgtg cgacagcact tcccatgacg cctgttccga taaaaccaag tttcattttt
    29401 tcgacctcct aaaaatcaaa aatccttgct tctatgctag tatacgctag aaacaaggat
    29461 tttttcataa aaaatgtttg aattttatct tttaaatgaa tttaatttta ttttgattcg
    29521 ttcaatcgag tcttcttctt cttttcttcg catctcttct tcatgataaa caaccatgtc
    29581 ttgataaaat tcgatcatag cttcgccgaa atgtgtagat gaaatatcat aaagtttagc
    29641 ttctaaaact tttggatctt taggaacttc actggaaata taatcaataa gtgctggtgc
    29701 aaagtcttcg tcggtttcaa aagttttccc taatgaagga tgatcgaaca atcggttcaa
    29761 ataagcattt ccttccacga cacaaggtgt tccggctgcc atcgcttctg tataagtaag
    29821 cccttgtgtt tctgaagtag aagcactgac aaaataatct gccgctttat aataaagtgc
    29881 tacatcattg ttgtccactt ctcctgtgaa ctgcacaaat tcttctaact gcagttctga
    29941 gatcaattcc tctaactctt ctacataagg tccttttcct acgataacta atctggcgtt
    30001 aggataagcg gagatgacct gaggaaaccc attaatcaag gcttggatat ttttctcata
    30061 agaaatcctg cttaaagaaa gaatcatcaa atggttttcc tgaagaccta gtgactccct
    30121 taatttgctg atatcctgct gagtgatatc agaacgtttg aatttttcaa tgtcgatacc
    30181 agtaggaatc acgcgaagcg gtgctattac accatagctg cgtaatgtat caataacacg
    30241 ttcacttgga caaactacac ctgtcgtatg atttgcaaat aatctagaca agtattttac
    30301 atgggattgc ctgatcactt ttcctttacc gatataatga aggtagtctt catacatcgt
    30361 atggtaagta tggatcagag gtattttcaa cttcttagct accatcttcc caagtatccc
    30421 tgctccgaat tctgtatggg tatgaataag atctagttct agttctttgg caaccaaata
    30481 agcataccac atgcctctaa caacaactct tcgatccttg aaagaaataa atggtacact
    30541 tggcattcga atcacatctt tttccaactt atccgcatta ggatcggtcg ttgtaaaaat
    30601 atagacttca tgcccatgtc tttctaattc ttgttttaat gttttgatcg atgtagcaac
    30661 tccgctgact tgcggaaagt aggtatcggt aaaaaagccg attttcatgt ttttcgcctc
    30721 caaactagtc cttgacctga tttacttttt ttaatttgct agagactttt tcattcagta
    30781 cactttcgta tacattttta agttcttcac cgattttatt gatacttcgc tcttttgctg
    30841 tttgataacc ggcttgtgtc gtactcggta gttcttgatt ggtaatctgt tggatcagtc
    30901 ggataaattc ttcgtttgtc ttccccatat agcagttctt gctatcttct aaccagccat
    30961 tatacactgg aatatctcga acaagtacgt tttgtttgct tgctaaagct tctaaaacaa
    31021 cgatcccttc tgtctcttca taagaaggga aaaagaatag atctgcacct gaatacgcac
    31081 cttctatgat ctctccacga atgtaaccag gaaacaggac attttcagga tgatcttctt
    31141 ttacaatttt ccggatgttc cgaggaatcg agtacatggg aacatgacca aaccaaataa
    31201 atgtgtattc gggcatcttc ttagcaattt ctacgaaatc aatgattcct ttacgttcga
    31261 aaaacaaacc gacacaaatg attacttttt gatccggaga cagtttgaaa tattctctga
    31321 acttttgttc tttcattgga tcagggtgat atttctctaa gtcgatccca ttggaaatag
    31381 cttgaatcgg cacacggata ccatagcttt ccaacaactg ttttgaataa ggtgttggcg
    31441 tgatcagata gtccgctttt tggtacaatc ctaccagata tttttttacg ataggagaaa
    31501 gctggttcga tccgataaag gaatttcgga aatcttcctc tgtagaatgc gcatgataga
    31561 caactttttt tccttctctt ctggctttat tgaccatatt atgactattg attccgtatg
    31621 tattgatgtg caggatgtca taatcttctt tttcatctag tgtataagag ataccgactt
    31681 ccgtcagagc acgtttttga tgatcaagcg cacgcccgat tccagatttt gcaagcatct
    31741 tttcactttc aaaatataat aataccttca ccttacgtcc cccttcactt atatagtata
    31801 acatagccta caaactgtca ctcaaactta agcc
//
LOCUS       DACDXO010000027        31696 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E23605_2017
            SAMN12639989-rid9881463.denovo.027, whole genome shotgun sequence.
ACCESSION   DACDXO010000027 DACDXO010000000
VERSION     DACDXO010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639989
            Sequence Read Archive: SRR10023389
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741914.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:42:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,972
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 33 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 17 of 73
            Pseudo Genes (multiple problems)  :: 10 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31696
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639989-rid9881463.denovo.027"
                     /isolate="E23605_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            363..731
                     /locus_tag="IWM35_001440"
     CDS             363..731
                     /locus_tag="IWM35_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HAP9919300.1"
                     /translation="MFTNEIKIMLYVDDVEKNATFWRAVGFAEKDRQEMDGTLIIEVG
                     VEDSQTSFLLYDRAFVESHSPGTATSAPSLMFYSKDIFGLYKKMQTQSVTLGDMVQLG
                     EEYVFNFADPDGNYFAVTGK"
     gene            911..1852
                     /locus_tag="IWM35_001441"
     CDS             911..1852
                     /locus_tag="IWM35_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HAP9919301.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            1921..2652
                     /locus_tag="IWM35_001442"
     CDS             1921..2652
                     /locus_tag="IWM35_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HAP9919302.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            2796..4976
                     /locus_tag="IWM35_001443"
     CDS             2796..4976
                     /locus_tag="IWM35_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HAP9919303.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            4996..5478
                     /locus_tag="IWM35_001444"
     CDS             4996..5478
                     /locus_tag="IWM35_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HAP9919304.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            5494..6255
                     /locus_tag="IWM35_001445"
     CDS             5494..6255
                     /locus_tag="IWM35_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HAP9919305.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            6386..7720
                     /locus_tag="IWM35_001446"
     CDS             6386..7720
                     /locus_tag="IWM35_001446"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HAP9919306.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            8027..8512
                     /locus_tag="IWM35_001447"
     CDS             8027..8512
                     /locus_tag="IWM35_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydratase"
                     /protein_id="HAP9919307.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            8505..9188
                     /locus_tag="IWM35_001448"
     CDS             8505..9188
                     /locus_tag="IWM35_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HAP9919308.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGITLAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            9314..11959
                     /gene="polA"
                     /locus_tag="IWM35_001449"
     CDS             9314..11959
                     /gene="polA"
                     /locus_tag="IWM35_001449"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HAP9919309.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELAEKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            12004..12840
                     /gene="mutM"
                     /locus_tag="IWM35_001450"
     CDS             12004..12840
                     /gene="mutM"
                     /locus_tag="IWM35_001450"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HAP9919310.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            12804..13433
                     /locus_tag="IWM35_001451"
     CDS             12804..13433
                     /locus_tag="IWM35_001451"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HAP9919311.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEKLVGQIENWLFANRFL"
     gene            13485..13817
                     /locus_tag="IWM35_001452"
     CDS             13485..13817
                     /locus_tag="IWM35_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HAP9919312.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHIPDEQKIEEQRMKH
                     YTGIEEKL"
     gene            14129..14620
                     /gene="nrdR"
                     /locus_tag="IWM35_001453"
     CDS             14129..14620
                     /gene="nrdR"
                     /locus_tag="IWM35_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HAP9919313.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            14643..16016
                     /locus_tag="IWM35_001454"
     CDS             14643..16016
                     /locus_tag="IWM35_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Replication initiation and membrane attachment"
                     /protein_id="HAP9919314.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            16016..16942
                     /gene="dnaI"
                     /locus_tag="IWM35_001455"
     CDS             16016..16942
                     /gene="dnaI"
                     /locus_tag="IWM35_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HAP9919315.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            17071..17511
                     /locus_tag="IWM35_001456"
     CDS             17071..17511
                     /locus_tag="IWM35_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAP9919316.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
     gene            17655..18356
                     /locus_tag="IWM35_001457"
     CDS             17655..18356
                     /locus_tag="IWM35_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HAP9919317.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            18353..19474
                     /locus_tag="IWM35_001458"
     CDS             18353..19474
                     /locus_tag="IWM35_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HAP9919318.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            19489..20721
                     /gene="pepT"
                     /locus_tag="IWM35_001459"
     CDS             19489..20721
                     /gene="pepT"
                     /locus_tag="IWM35_001459"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HAP9919319.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            20853..21761
                     /locus_tag="IWM35_001460"
     CDS             20853..21761
                     /locus_tag="IWM35_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HAP9919320.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            21796..22068
                     /locus_tag="IWM35_001461"
     CDS             21796..22068
                     /locus_tag="IWM35_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HAP9919321.1"
                     /translation="MDVLLHNPLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            22079..23125
                     /locus_tag="IWM35_001462"
     CDS             22079..23125
                     /locus_tag="IWM35_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HAP9919322.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
     gene            23376..25985
                     /gene="clpB"
                     /locus_tag="IWM35_001463"
     CDS             23376..25985
                     /gene="clpB"
                     /locus_tag="IWM35_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HAP9919323.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQSNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(26136..26807)
                     /locus_tag="IWM35_001464"
     CDS             complement(26136..26807)
                     /locus_tag="IWM35_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9919324.1"
                     /translation="MIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGITFIVES
                     LLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWDKILHA
                     VSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLCDQFLG
                     MNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFESYKLE
                     KVKNT"
     gene            complement(26800..27294)
                     /locus_tag="IWM35_001465"
     CDS             complement(26800..27294)
                     /locus_tag="IWM35_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HAP9919325.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            complement(27314..28534)
                     /locus_tag="IWM35_001466"
     CDS             complement(27314..28534)
                     /locus_tag="IWM35_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9919326.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            28681..30516
                     /gene="lepA"
                     /locus_tag="IWM35_001467"
     CDS             28681..30516
                     /gene="lepA"
                     /locus_tag="IWM35_001467"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HAP9919327.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEDEPKK"
     gene            complement(30610..31692)
                     /locus_tag="IWM35_001468"
     CDS             complement(30610..31692)
                     /locus_tag="IWM35_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017414133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP9919328.1"
                     /translation="MNDDGSRKKVEKVGGDTRPEAEAALRKVLSDIDETGQYFLGTDT
                     RVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKLTDLSPALLQNFINAE
                     FKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPKYEARPTTKKDLKIIS
                     LEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVDFSNGTITVEKQMVKN
                     DGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYGKLYIDSNAVCTKEDG
                     ELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKMKEISERLGHSRISIT
                     MDTYSHVTDKMRNETVDIMENLRKNS"
BASE COUNT        10548 a         5659 c         6786 g         8703 t
ORIGIN      
        1 agaaagaggt cgtgagcctg ccgtttcact ctgagtcaca aagagcaatt tcaaaaaata
       61 gcttctcatg tttgttgaat attgtgtctt gtgaccgagg agttgtcagg agaacaccgt
      121 ttaccagagg ttgtgacttg tttttagtca cgacctcttt ttttatttat tccatataaa
      181 tacacattat ttattccgaa aagtgaaagc gcaaataaca aaaacaacca acttatttat
      241 tattaaagaa aagcgtattt aagtaggacg attctgttga gagctttcgt agattttccc
      301 attttcctat ggtaaactga caaagttgag aatgttagaa cagatcgttt aggaggaaac
      361 agatgtttac gaatgaaata aaaatcatgc tttatgtgga cgatgtagaa aaaaatgcaa
      421 cgttttggag agcagttggc tttgcggaaa aagatcgtca agaaatggat gggacgctta
      481 taattgaagt aggagttgag gacagtcaaa cctcatttct gttatatgat cgagcatttg
      541 ttgagtccca ttcacctggc acagctacta gtgccccatc tttgatgttt tacagtaaag
      601 atatctttgg actatataaa aaaatgcaga cgcaatcagt cacattaggt gatatggtac
      661 agcttggaga agaatatgtc tttaactttg ctgatcccga tggcaactac tttgcggtta
      721 caggtaaata atagaaataa agactttgtt tttctgtgcg aaacaggaaa gcgaagtctt
      781 tttaattcaa ataataatat tatgtaaaca aaaaactttc catgcgtaat tatacacaag
      841 caaataaatc gtcaaatgcg aacgccttct ttatactgtt taggaacgaa aagaaggaag
      901 gaagaaatac atggattatc aagtactgct ttattacaat tatacaaaaa tcgaagaacc
      961 agaggaattt gccaaaaatc atttagcctt ttgcaaatct ttggatctta aaggtcgtat
     1021 tctagtagct gaagaaggaa taaatggtac tgtgtctgga accgttgaaa acacgactgc
     1081 ctatatgaaa gcaatgaaag ctgatcctcg atttgctgat acgatgttca agatcgatcc
     1141 aagcgaagga catgccttta agaagatgtt tgtaagatac cgtccagagc ttgtaagttt
     1201 aaagctagaa gaggacattg atccaaacca agtgacaggt gcctatcttt caccgcaaga
     1261 atttaaagaa gctattttag atgaacagac agtggtgatt gatgcacgga atgattatga
     1321 atatgacttg ggacattttc gaggggctgt acgcccagat atacgcagtt ttcgagagct
     1381 tccagactgg atccgcgagc acaaagaaga atttatggaa aaacgggtag tgacctactg
     1441 tactggtggc attcgttgcg agaagttttc tggttggctt gtccgtgaag gattcaaaga
     1501 tgtcggacag ctgcatggcg gtattgctac ttatggtaag gatcctgaag tccaaggaga
     1561 attatgggac ggcaagatgt atgtgtttga tgaacggatc gctgtcgata tcaaccaagt
     1621 agataaacaa atcgtcggtc gcgactggtt tgacggaaca ccatgtgaac gatatatcaa
     1681 ttgtagcaat cctgagtgca atcgtcagat tcttgcttcc attgaaaatg aagaaaaaca
     1741 tttaggagca tgtagcatag agtgtgcgaa acatccgcgc aatcgctatg ttcaagacca
     1801 tggaatctct ccagaagaag cagaactgcg gtttacagct ttaattaaat agaaaacaat
     1861 agttttgatt gtcgaccatt tccaatatgc gctatactag cgttgaggat ggtgatccac
     1921 atgaaatcat tgatttatgc tattggggat gtccatggca aacatgagct ttttgaaaaa
     1981 cttattcact attatgatcc aaagatccat cagcttgttt tattagggga tctcaatgat
     2041 cgaggtcctc gtacaaaaga atgtttttta ttaggaaagc aattagtcga agaaacagga
     2101 gccatttacc tccggggaaa ccatgaagaa tattttttgc aatttctgca atcaccggag
     2161 gactggtatg cgccttatat gagaaatggt ggaaaagaaa cgtttgaaag cctgattcac
     2221 ccaggggcat cggaggaata ctccccaact gaaatctcga tgatgatcca atcgagatat
     2281 tcagaactga ttaaattttt agtccagcgc cccttgtatt atgaatggga aaattatctt
     2341 tttgtccatg ccggtgtgga tttgacaaag aaagactggc gtaagacaag tctaagagac
     2401 tttatttgga taagggaacc atttcatgaa gggaaaaaca atacaggtaa gacaattgta
     2461 tttggacata cgattacacc aatgctccat ggagatatgc agacaacaga cctctggatt
     2521 caggatcata aaatcggaat ggacggtggg gctgtattcg gagggtcatt gcatggggtt
     2581 gtttttgaca aggaaggtat tcttcaagat attgaatatc agaacaacga aggaccttgg
     2641 catggaatct gactaatgga aagaatgaag gagctgtgac tttgttgcgg ctctttttat
     2701 ttttatagag aatattgttt ctttcagtag tttaacccag gataaaaagt atgatagaat
     2761 aaagaagcaa tttttagcag aaaagaggca ttttaatgac ggaaaatttg aaccagacga
     2821 tcattcagct tgtacaaaaa gaactttcag actatcgtcc taagcagttg actactgtac
     2881 ttaatttgtt aaatgaagga aacactgttc cttttatcgc gcgttaccga aaagaaatga
     2941 ctggaagtct tgatgaagtc cagattcgtg aaatcgaaga gcggtatgcc tatcttgaaa
     3001 acttagaaaa acgtaaaact gaagtgattc ggctaatcga tgaacaaggg aaactgactc
     3061 ccgagttaga agcagaaatc acccaagccg tgaaaatgca acaagtggaa gatttgtatc
     3121 gtccatacaa acaaaaacga cggacaaaag caacgattgc taaagaaaaa ggacttgaac
     3181 cactcgcgaa gtggctgatg cagcttacag atggagaggt gcaatctgaa gcagaaaaat
     3241 atattgacaa agaaaaagaa gtctcttctg ccgaagaagc gttgcacggt gctcatgaga
     3301 ttattgctga acaagtcagc gataatgcaa aatttcggac ttggattcgg tcttatacgt
     3361 ataacaaagg tatgtatgtc agcaatgtca aggatgagca agcagatgaa aaaggcgtat
     3421 atgaaatgta ttatgacttt gcagaacccg tccataaaat ggtttctcat cgaatattag
     3481 ctactaatcg tggagaaaaa gaagatattc tgaaagtatt ctttcaagta gatgaagcag
     3541 caattttagc ttacttggat cgccaaatag tcaaaaatcc tgcctctcct tcttcttcat
     3601 tcgtcagaga agcctatcag gatagttata aacgctttat tcagccagca atcgagcgag
     3661 aactgcgcaa tgaattgact gaaaaggcag atgagcaagc aatcgctatt ttcggcgaaa
     3721 acctccgtaa tcttttgttg caaccgccac taaaaggtaa agttgtcctg ggatttgatc
     3781 cggcttatcg tactggatgt aaactagcag tcgtagatgc gacagggaaa gttttagcaa
     3841 tcgaagttat ttatcctcat aaacctgcag cgcaagcaaa aagagaagct gctggaccag
     3901 cttttatcca attaattaat aaatatcaag tagatatggt agctatcgga aatggtacag
     3961 ccagtcggga atctgaactt tttgtggctg agcagttgaa atccgccgac cataaaacct
     4021 attacgctat cgtcaacgaa gcaggcgctt ctgtttactc cgctagcgaa attgcacgaa
     4081 aagaatttcc gcatctacaa gttgaagaaa gaagtgctgt ttctattgcc cgtcgcctgc
     4141 aagatccttt agctgaatta gtcaaaatcg atcctaaagc tgtcggagta ggccagtacc
     4201 aacatgacgt ttctcaaaaa cgtttggctg aacaattgga ttttgttgtg gaaacagcgg
     4261 ttaaccaagt aggtgtggat gtgaatacag caagcccgca gctcttgcag catatatcag
     4321 gattgaataa aacaactgcc caaaacattg tcatttatcg tgaagaaaac ggagaattca
     4381 ccgcacgaac tcaattgaaa aaagtgccac gcttaggacc aaaagcatat gaacaagcaa
     4441 tcggattctt gcgagtccct ggtgggaaaa acattttgga caacacaggt atccatcccg
     4501 aaagttattc tattgcaaaa gaaatattaa cttcagtcca actttcagaa aaagagcttg
     4561 gtacagaaga agcagtagaa aaattgacac gattatccgt agagaaactg gctgaaagcc
     4621 tatcagtcgg agaagaaacg ttagccgata ttttagctgg gctgacacaa cctggacgag
     4681 atatgcgtga tgagatgcca gcgcccttat tacgtacaga tgtattgagt atggaagact
     4741 tgaaaccagg gatggaattg actggaacgg tccgtaatgt cattgacttc ggcgcatttg
     4801 ttgatatcgg tgtgaagcaa gacggacttg tccatatatc gaaactcagc aaaaaattcg
     4861 tcaagcatcc tacagatgtc gtgtcagtag gagatatcgt aacagtttgg atagaacaag
     4921 tcgatacgaa aaaaggtaga atcagtttga cgatgctttc cccttatgaa gagtaacaat
     4981 gaggagtaac aaaaattgac acaagaagaa ctgcagcagt tagtagaaga gatatcatta
     5041 aaaagcttcc agcttccttt caagcaccaa gcaatgttca atccacgctt acgtacaact
     5101 ggcgggcgct atcatttaaa cgatcatcat ttagatttta atgcgaagct atttgaagag
     5161 tcagacgaag agacgattgc aggaatcatc aaacatgaac tttgccatta tcatcttcat
     5221 ctagctggaa gagggtatca gcataaagat atggatttca agtatctttt gaaaaaaaca
     5281 ggagggttgc gctatgcacc agccttgacg aagaaacaga taaagatgga gtattatcga
     5341 tgccaagatt gttctgccac gatctgccgc aagagaagaa tcgatgtacg caaatatcgt
     5401 tgtggaagat gtcatggaga aatcaaatgg attgagacaa aaagagaaga ctttaatgaa
     5461 gcacaaaaaa tcagatgaaa ggatagaagg cctttggaaa aaagtgagca gaggaatcta
     5521 aaagactgga atcagctatt aaatttttta gccgcacctg aagagggaaa ttttgaggaa
     5581 ctgacggcac ctacacttat tttagctgga aactgtcttc ctatcttagc tgataaagca
     5641 gcacaaatgt atttaaatgg ccaagtagat caattgtttt tggtcggagg ggtggggcat
     5701 gcgactagaa tcctttatga gaattttgaa aagcaaggat ttcattttga agatggcatg
     5761 agtgaaagtg aaatctgtcg tcagtatcta aaagaagtct atgatttgcc cgataaggct
     5821 tttctgatag aatcgaagtc tacaaattct ggagagaacg ccactttttc actggagatt
     5881 cttcattcct taggagctgt accagaaaaa atcttattga tgaatgatcc gacactgcaa
     5941 cgcaggactc gagcaacttt tgagaaagta tggcaaaatg aacaaactat tttcgcgaat
     6001 gctgtgccat ttgtgccgga aatccttcac ttttctgaag agatcatctt tacagcaaaa
     6061 gaactgaatc atcaatggcc taaggaatat ttccatgcgc tagtattagg agaaatggaa
     6121 cgtctccatg acgatgagaa cggctatggt ccgaaaggaa aagattttat tcctcacatt
     6181 gatatatcag aggaagtttg gtcttcttac accagaatta aagagtcaat aaaaacggat
     6241 ttttctcgta cttgaccatt ttagcaagcg aatcctcctt tccaaataaa tttggttgaa
     6301 attttcaatc gtttataaga taatgaggag cgtgttgaat gtaatagcta aaagaatgga
     6361 cgctagataa gagaagagga ttcaaatgga aaatcaaaat aaattgtatc attttgttgg
     6421 tatcaaaggt tcaggaatga gctccttggc tcttgtttta catgaacaag gtctaaatgt
     6481 ccaaggatca gacattgaaa aatatttctt tacacaaaga gatttagaaa aagcaaatat
     6541 cacgattctg ccatttaacg cagataatgt aaaaccaggg atgacgatca ttgcaggaaa
     6601 tgcatttcct gattcacatg aagaaatcca gcgagcaaaa gaattaggtt tggaagtgat
     6661 tcgctatcac gactttattg gtcattttat ccaaaattat acgagtatcg ctgtaacagg
     6721 ctctcacgga aaaacaagta cgactggact gctctctcat gtattgagtg gtgtccgtcc
     6781 aacaagttat ctaattggag atggaacagg acatggtgat ccgcaagcgg aattcttttc
     6841 atttgaggcc tgtgaatatc gccgtcattt ccttgcctat tcaccagact atgcaatcat
     6901 gaccaatatc gattttgatc atccggatta ttatacaagt atcgacgatg tatttacagc
     6961 tttccaaaca atggctggac aagtgaaaaa agctattttt gcgtatggtg acgatgctta
     7021 tttacgcaaa ttaaaagcaa atgtgccgat ttattattat ggtgtcacag aaaacgatga
     7081 tatccaagca cgaaacatcg aacgcacaac ttctggttct gcttttgatg tctatcatgg
     7141 ggatgaattt gttgggcact tcactgtacc agcattcggt aaacacaata tcttaaatgc
     7201 tttaggtgtt attgcagtag cttatttcga aaaactggat ctgaaagaag ttgcagaaga
     7261 aatgctgact ttcccaggag taaaacgtcg attcagtgaa aagatcgtag cagacatgac
     7321 cgtagtcgat gattatgcgc atcaccctgc agagatcaaa gcaacgattg atggcgcacg
     7381 ccaaaaatat ccagataaag aaataattgc agttttccaa ccccatacgt ttacgcgtac
     7441 gattgcattg atggatgaat ttgcagaagc acttgattta gctgataaag tttacttatg
     7501 cgatattttt ggttcggctc gggaagaaca aggaaacgtt aagattgaag accttggagc
     7561 gaaaatcaaa aaaggtggag aagtcatcaa agaaaataat gtatctccat tgttagatta
     7621 tcatgatgca gtagtaatct tcatgggtgc tggagatgtt caaaaatttg aacaagcgta
     7681 tgaaaaactt ttaagcagca cgacgaaaaa tgtactgtaa tacaattact gcagtgtcct
     7741 tataaaaata gaaagagggg gagtggcaaa aatcttgcca agcccccttt tttgcaactt
     7801 atgataaacg gtgttcaaaa gcttgcatct gcggtaataa gcccaatcaa atcaaaaaca
     7861 tgagaagctg ttttttcttg gctgttctta ttatttgatg caggacagct ttttcacgac
     7921 ctctgtaaac tataggaatc tgttaaatca agggagcaga acttgccttt tacgctactt
     7981 ttggtacaat aatgtatgat taaaaataaa ctggagggtt tctatcttga acataggtaa
     8041 gccgagaaaa ctaggaaaaa caataaatga aatcgaggaa ggcgactcgt tatccttaac
     8101 agaatctatt gaagataacc agcttttgct gtatttggga ttgacgaatg atgcaaatcc
     8161 tttgtatatc caacatgact ttgcacaaaa aacagaatat caacagccaa tcgttccatc
     8221 agtgatgctg atggggatca tcacaagtgc aatttcaaag catcttcctg gaccaggatc
     8281 acatgttgtc aatttttctg tcaattttat tgaaccggtt ttccattatg aaacactgac
     8341 ttttcagttt gaggtcataa aagtagataa gatgaaggat gtggtgacga tttctgtaga
     8401 agcggagaat acgaacgaag atcgtgtgtt ggatgctgtc gttatggtcc aaccgccaca
     8461 agttcctgaa ttggaaatag caatcgaaag tgaaggtgaa ttgaatgaat aatccaagta
     8521 catcaatgtc aggtatcaat cgtttttatg caaaagttta tggattttta ggtatgggac
     8581 ttgcccttag tgcagtcact tcttacttgg ctctttgggt atttcctttg caattagcaa
     8641 cttttatcaa taattttcca ctaggctttg tgggtctgtg gatcgttgaa ctgattttag
     8701 taatggttct aggcgtaaaa gcacaaaaga atccgagctt agcaatcggt ggatttattg
     8761 cctactctct tttgaatggc ataacattag cggttactct tgcatactac gatattggaa
     8821 cagtaacgca agctttcttg acagctactt tgatgttcat ggggatgtct gttgtcggag
     8881 taacaacaaa aagagatctt agcggggttg gaagagcagg acaggcagct ttaatcggag
     8941 tgatcattgc actagtcttg aacatcttca ttttgagaag tagcgtagtt gagctgttca
     9001 tctccctact gttagtcgtc atctttgcag ggattacagc ttatgataac caaagcatcc
     9061 gtaatatcta tgtccagaca aacggaacag cacatagtgg aattgctgta ttcatggcat
     9121 tgaagctgta tttggacttt atcaacttgt tcttggcgtt tctgagaatc tttggacgga
     9181 acaactaaaa aagaaaaaaa ttcatcagtt ggggctatac cgaattattt ggtatggtcc
     9241 cgctttttct tttcgcattc cgcaggattc tttgttagaa tgaataagaa ctttacagta
     9301 aggagttaag aagatgacaa aaaataaatt attactcgtt gatgggaaca gtgtggcttt
     9361 tcgcgcattc tttgcgcttc acaattctct tgaacgtttc aaaaatagaa acggtcttca
     9421 tacaaatgca atctatgcat tcaatacgat gtttgaaaat gttatgcaaa aagagcaacc
     9481 gactcatgtt ctggttgcgt tcgatgcagg aaaaacgact tttcggacag aaatgtatgc
     9541 ggagtacaaa ggtggaagat ccaaaacacc tggcgaattc aaagaacaaa tgccatatat
     9601 acgtgatttg ttgacaggac taggggtaca gtattacgaa ttgcccaatt atgaagcaga
     9661 tgatattatc ggtactttgg cagaaaaagt agacaaagat caatttgatg tcgtcgtgct
     9721 ttcaggtgat cgggatttga ctcaattagc tagtaaagaa gtaaaagtgg atatcactgt
     9781 aaaaggtgtc agcgatatcg aaagctatac accagaacat gtagcggaaa aatatgatgg
     9841 gttgacgcca aaacagatta ttgacatgaa gggactagcc ggagatgcct ctgacaatat
     9901 ccccggtgtg actaaaatcg gtgaaaaaac agctatcaag ctattgaaac aatatggctc
     9961 agtcgaagga atttatgaac atatcgatga aatgaagcaa agcaaaatga aagaaaatct
    10021 gataaatgac aaagagcagg ctttcttatc gaaaaaattg gcgaccatca atacttcagc
    10081 gcctgtggat gtctctatcg actcattgaa gtatgaaggg aaaaatctcg ataaactcgt
    10141 tccgttttat aaagaaatgg atttcaatca gttcctttct aaattgaata tcgtagaaga
    10201 agaagtaaaa atggatgata ttttgtttga agtcgttcat gaattcaaag aggaaatgtt
    10261 tactacggat atggcgttgt acgtggaaat gatgggcgac aattatcata cagaggaaat
    10321 cgtcggagta gcgtggggga cagaaaagaa aatttatgtc acgaacgatc tatctgtatt
    10381 cgaaaaccga gcgttccatt cttggatcac tgatccgact cggctgaaaa aagtatatga
    10441 tgcgaaacga acttatgttg ctttaaaccg ctatactgga aaaacgaaag gaatcgattt
    10501 tgatgtcttg ctaggtgcat accttctcga tacgaatgaa aaaagcacag atatcgaagg
    10561 aatagctgct cattacggat acaacgatat ccaatcagat gaagctgtat acggcaaagg
    10621 ggcgaaaaaa ggtttaccag aagaagagga actctttttt gctcatttag cgcgaaaagt
    10681 agctgcaatc aatgcgttga caccaaaatt gagccaagaa ctggcagaaa aaaaccaaga
    10741 agatcttttc cggaaaatgg aactgccatt gtctatcatt ttggcagaga tggaaataag
    10801 cgggatcaaa gtagatgcga ctcgcttaca agaaatgaag ggcgagttct ctgctcgctt
    10861 aagagagatc gaacaaaaaa tctacgaaga agccggcgaa gaattcaatt tgaattcacc
    10921 aaagcaatta ggagtgatct tatttgaaaa aatgggatta cccgttatca aaaaaacaaa
    10981 aactggatat tccacagccg tagatgtact ggaacagctt cgtgaacaag caccaatcgt
    11041 agaggatatc ctcacctatc gtcaaattgc aaaaattcaa tctacctatg tcgagggact
    11101 gctgaaagtg atccaatcag atggaaaagt gcatacccgt tatgtccaga cattgaccca
    11161 aacaggacga ttaagctctg ttgatcctaa tttgcagaac atcccgattc gattagatga
    11221 agggagaaag atacgtcagg catttgttcc tagagaaaaa gactggctga tttattcttc
    11281 tgactattca caaatcgagt tacgcgtact tgcgcatatc tcaaatgatg aacatttgaa
    11341 agctgccttc ctagaaggtc aagatatcca tgccagcaca gcgatgcgtg tatttggaat
    11401 cgaaaaggca gaggatgtca caccaaatat gcgtaggcag gcaaaagcag tgaattttgg
    11461 tatcgtgtat ggcatctctg attacggact ttcacaaaat ctaggaatca gccgaaaagc
    11521 agcacagcaa tacatcgata cgtactttga aaaataccca ggtgtcaaag aatatatgga
    11581 aagaatcgta cgcgaagcaa aagaccaagg ctacgtcgaa acgctgtatc accgccgtcg
    11641 atatctgcca gacatcaatt caagaaatta caatatccgt tcttttgcgg aacgaacagc
    11701 tatcaataca ccaatccaag gaagtgctgc tgatattttg aaaatcgcga tgatcgaatt
    11761 ggataaacga ttaaaagaaa caggtcttca agctacgatg ctgttacaag ttcatgatga
    11821 acttgtcttc gaggtaccgg aaaaagaact tgaatcatta gataaattgg tgaaggaagt
    11881 tatggaacaa gctgtttctc ttcatgtacc actgatcact gacagcagtt ggggaaaaac
    11941 gtggtacgaa gcaaaataaa cattgtttaa gacagagcaa tataatgaag gtgaaaagaa
    12001 aaaatgccag aattaccaga agtagaaacc gttcgtaaag gattggaacg gttagtagta
    12061 ggaaaaacga ttcaaaaagt tcaggtcttg tggcccaaga tcatcgagca accagagaca
    12121 cccatctttg aggcttctct tgttggcgaa accatccaat ccattggacg tagagggaag
    12181 tttttaattt ttcatctcga tcattgtgaa ctgatctcac acttgcgaat ggaaggaaaa
    12241 tatcaattta cgaaagaaaa tacaccaatc gataaacata cgcatgtact tttctttttt
    12301 gaggatggaa gccaacttcg ctacaacgat gtacgaaaat ttggtcgtat gacaatcgtc
    12361 gaaaaaggag caagtgcaac ttaccgagga atcatgaaat tgggacctga gcctttgcca
    12421 gatagcttct tattagcaga tttcgcaaat ggactgaaaa agtcccacaa agcaatcaag
    12481 ccattgctgc tagaccaacg tctagtgact ggtctaggta atatttatgt agatgaagca
    12541 ttatgggaag cgaagatcca tccagaacaa ccggcgaaca cactgcgccc caaagaggta
    12601 gaacaattac gtctatcgat cattgatgtt ttggatcgtg caatagaagc cggaggtacc
    12661 acgattcgca gttatttgaa tgcattagga gaatcaggcg gatttcaagt agcactgcat
    12721 gtctatcagc aaactggcaa accatgtata cgttgtggaa cgccgatcgt gaaaacaaaa
    12781 gtggcacaaa gggggacgca ctattgtccg aattgtcaaa ggctaaaaat cgcaaagtag
    12841 gatttgtttt aggtttgacg ggtggaatag ctaccggtaa aagtacggca gccaaagtct
    12901 ttcagtctca tggatttcct ttgatcgatg gcgatgtgat tgccagagaa actgttgagc
    12961 ctggaacgct tgctctgagg aaaattgtat cagtgtttgg ccaagaggta ttgcagcctg
    13021 atgggcaact agatcgaggg aaacttggga tgattgtttt tccatcaaag gaaaaacggc
    13081 aaaaacttga tcagctatta gatccgttta tccgaaaagc gataaaagat caaatcgcct
    13141 ctctaagttc caatcatcct ttagtaattg tagatatccc attgctctac gaaggtcatt
    13201 atgaccacta tatggatgca gtagctgtcg tttatactac gccagaaact cagcttcagc
    13261 gtttgatgaa acgtaatcaa ctgacgctag aacaagcgca gcagagaatt agtagtcaac
    13321 ttccaatcga agaaaaaaag cagcgtgcgg atattttgtt cgacaacaat ggaacaaaag
    13381 aaaaacttgt gggacaaatc gaaaattggc tttttgctaa tcgctttctt tgagtatctg
    13441 tgtcaatttt gctatcctga gtatatagaa agtgagggat agcgatggga tcattaaaaa
    13501 atcaaaagga taaagtcgta ggtaacgcaa aagaaaaaat cggcgagatg ctcaataaag
    13561 aaaatctaat cgatgaaggt cgtacgcaat cagaaacagc agaagaaaaa gaaaagcagt
    13621 accaagcaag aatcgatgaa atagaagacc agcaggcaga agaagaagcg aagcggcaag
    13681 atttgactca agaacgaaaa aatccgacac ctgatcgtag taaaacaccg ctcccagaaa
    13741 gtgaccacat tcccgatgag caaaaaatcg aggaacagcg aatgaaacac tatacaggaa
    13801 tcgaagagaa gttataaaat agtaaaaaat cgagatgtga cttgaagcag atgagaaggt
    13861 gttcatctgc ttcaagtttt ttgtgctaga tgactaaatc agcttttgtg taagttattt
    13921 ctcagtaatc aaggtgatca gtattggaaa attgaaattt agtaaggaca ggggacacaa
    13981 ttacgcatga aagtagaaaa aattgttctt atatccgtgt tttttgggta ggacagtcgc
    14041 gtttgtgatt tgtttcgtgt tataatacaa ctagttgtaa agaaagacac atcctttata
    14101 taaaatgcga tgcaagaggt gagacaaaat gagatgtccc agatgtcaac ataataattc
    14161 acgagtaatc gacagccgtc aagcagatga tggacgtgcg atccgtcgcc gtcgcgaatg
    14221 tgagaattgt tcgttccgat tcactacatt tgaacgaatt gaagctgctc cgttattagt
    14281 aatcaagaaa aacggggaac gcgaagagtt caatcgcgat aaaattttaa gaggactcat
    14341 ccgttcagca gaaaaaagac ctgtagctat ggaacaaatg gaacaaatcg tcaaccatgt
    14401 cgaaaaccgc gtacgcagtc tcggagaaaa cgaagtatcg acgaatctaa ttggtgaata
    14461 tgtgatggaa gatttagttg atttagacga aatcgcttat attcgttttg caagtgttta
    14521 tcgccagttc aaagatatgt ctgttttctt aaaagagttg caagatatcg tggataaagc
    14581 aaaagaaaat aataaagaga ataataaaga aaacaattaa gaaacaaaaa ggaggcattt
    14641 ccttggaaaa tgcttggaaa gagcttcaac caaaaaatat ttaccaagga agaaaagcgg
    14701 ctccgctgag agaagacagc aagaacgcct tgctgtatct ttatcagcca ctaatcggcg
    14761 gagaagcctt gagtatctat ttcaccctgt tatcagaaat ctctcaagaa tctggcaaag
    14821 gtcctgaagg gatgcatgca gatctgctta gtagtctagg ctgcggtatc ccgcaatttt
    14881 atgatgcaag aaaaaagcta gaaggaatcg gcttactgga tgtttatttt aaagaagata
    14941 gtgatctggg acctcgtttt atttatgagc tgttagaacc actcattcct aaagcatttt
    15001 tcaaagactc tgttctggct tttcttcttc tggaaaaagt tggtgaacga cgattccaga
    15061 agcttaccca attctttgaa cctaaaaggc atacagcaga gggatatcaa aaaatcacaa
    15121 aaaaattttt agatgtttat cgcttcaatg aagcagtgta cgctgctaat caagaaaaca
    15181 tcgaatcgct gaataaatct tttgaagaat ctgaatcagt agcattactt gaagcagatt
    15241 cgacattcga ttggcagttt ttgaatgatt ggctggcaaa aaaacatatt gcaagtcttg
    15301 atgatccaat catcaaacaa gtcgacatgt atcatcagct ttacggtttc gatgaattga
    15361 cacttggcga actgatcagt caatcttatg actttagtga acaaaaagta tctgaaaaag
    15421 aactgcaaaa agccattttg gcaatgcaga gacgtccggt gaagcaaaca aaagaaacag
    15481 ttgcggtttc tgaaaacagt aatgaaccct cgtcaaacga acatttgaca aacgcagcgc
    15541 ttcagctgat aaaagaagca cagagcattc caccgatgaa atatctggaa gcaatcaagt
    15601 cagaaaaagg cggatatgtt tctaaacaag aaaactggtt actgcaagat cttgtttctc
    15661 aatcaggtct ttccagtagc gtgatcaatg ttttgctgaa ttatgtacta gtgatcaaga
    15721 ataatgctag cttaaatgcc agttatgtaa atacagtggc aaatgaatgg gcacaaaaga
    15781 aaatcgccac agcagaagaa gcgatccagc acattcggca aataagtaat caagcaaaac
    15841 aatcaaaaca aaagaagcaa acaaattatc aaactggcaa gcgaaatgtt cgacgggaaa
    15901 agcttcctga ttgggtcaac cagccaaaag atgaaaaaca gatcagcagc gagaaaaaag
    15961 cagagattga ccgccgtttt aaagaatacc tggcaaaaaa ggagggtgat atctagtgga
    16021 agacgtaggc aaagaattga agaggatcat cagcaaacgc aattatcaag aaaagtatgc
    16081 tgaaatgatc gctgaagtct taaatgatgc ggatgttcaa gcttttctaa gcgcacacca
    16141 agaagaattg acacaagcgg atattgaaaa aagttacgct aaattgtatg aattcgtgca
    16201 agaaaagcgt aaatatcaaa aaaacgatcc aacgatgatc gctcccggct atgagccgaa
    16261 gctgacgttg aattttcatt atgtggatgt cacttatgta ccaacggaag cattgttagc
    16321 acggcaaaga caagaagaaa tcagaagccg agtaaaagca atggatatgc caaaagatat
    16381 tcaatcggct tcttttgaaa actttgaacg tacagatgga cgaggatatg cgagcttaga
    16441 agccctagat tttgtagaga aatatactgc tgatccaaaa gggtatcata aaggactgta
    16501 tctagtagga agttttggta ttggaaaaac atatttatta ggagcaacag cgagagaatt
    16561 agcgattgct ggattcacca cgacgctagt ccattttccg acatttgccg ttgagatgaa
    16621 acaggcaatc ggaaaagatc aagtggcaga aaagttagat gcagtgaaac gttcacctat
    16681 tttgatgatc gatgacattg gtgcagatgc gatgagtagt tggatacgag acgaagtatt
    16741 cggtgttatc cttcaatatc ggatgcagga acagttacca accttcttct catcgaattt
    16801 tacaatggat gaattggaac agcatttgtc cgtcactcaa cgtggagatg aagagccgct
    16861 aaaagcgaaa cggataatgg aacgcatacg ttatttgaca aaagaaatcg aaatgactgg
    16921 tcgcaaccga cgcaatggtt gaacagttga caagtccaac tgacatttga cctagtagta
    16981 aaagcaggtc agcgaatcat tatatggaca gaaatgaccg gagacaatca ccgcaacaag
    17041 taatgcagaa acttaaacgg aggatttttt atgagggcta ttttttctag tatatctgta
    17101 gttggtctat tttttctttt agcaggctgc ggtagcgcag atagcaacac aggttctgtt
    17161 gattcgattg atgcgggtca aacagtaaaa acaagcagta cgaaaacgga gagacaaagc
    17221 atatttgtcg gaacgattga aagtatcact caagtagaag cagaagaaga aacgcttcag
    17281 ttgaaactcg tcgatgtcat ggccaaagaa gatccagcga atatgggaac gtcattcagc
    17341 aatgacggag tgatcttaaa tgctactgct gaaaaaataa gcgtggacat aaatgaattg
    17401 aaaatcggtg acaaagtcca atttacatta gttgagatgc cgatcatgac gatgtctatc
    17461 ccaccgcaag ttcctggaga ttcgattatt gatgtttcat tgaacaaata accaataaca
    17521 ttttttcaaa aataagaaaa tgttgtttta tagaacgaaa aggtatattt tttaaggaga
    17581 tatgggaatt tttttattcc tagtctcctt attttatttg gaaaatgcta taattatcta
    17641 gaaggagtga agctatgaac catttagatt tatctcagcg cttagccgca gtgggtgact
    17701 ttgtccctga atccgctcgt ttagcagaca ttggatcaga tcacgcttac ttacccgttg
    17761 cattgatatt aagaggaaaa atcgaatatg cagttgctgg tgaagtcgtt caaggtcctt
    17821 atgaatcagc acgtaagcaa gtcaaaaaag atggattaga agaccgtatc accgtacgcc
    17881 ttgcaaatgg tctagatgct atccagccac aagatggtat tacagctgtc acgattgcgg
    17941 gtatgggagg ttcactgatc cgagatatcc tagaagctgg caagttgaat caacgcttaa
    18001 gcggacaaga acgtttgatc cttcagccaa acatcggaga gcgtacatta cgatcttggc
    18061 tgcaagaaaa tcagtataaa atccaatcag aaaaaatact tgaagaaaat aaaaaaatat
    18121 atgaaatcat tgtagccgaa aaagaacaaa aaccagttac ttacaatgaa atggagctga
    18181 tgttcgggcc atttctgctt gaagagaaaa gtgagatctt caaatcaaaa tggacaagag
    18241 agcttcaaca aagaaaagct atccgtgaac agctcaaaca agcagctaat caagaggcac
    18301 gtcaaaaaca aatcgagcag gaaatccaaa tgattgagga ggttttaaag ccatgacaat
    18361 cagcggacgc actttcatcc aaaagtttga aagctattgt cctcagtggc tagctgaaga
    18421 aggagaccca gtcggactgc atatcggaac attggataag ccaatccaac gagtaatgat
    18481 gacattagac gttcgtccag aagtggtcga agaagcaatc aagaaaaaaa tcgatttgct
    18541 gattgctaaa catcctccaa tctttcgtcc tgtaaaacgg ctggttactg atcagccaca
    18601 agaaaaaatg tatgcagatt tgttgaaaca tgatattgcc gtctatgcag cacacacgaa
    18661 catggatatt atttgggatg gactgaatga ctggttttgt gaacttctcg gtattgaagt
    18721 ggagagttat ttagtaaaga ctcatgagat tcattataaa aaactggccg tttatgtacc
    18781 tgtggatcat gcacagaaaa tgcgtgaagt attagctgca gctggtgctg gtacacaagg
    18841 tgattatacc ggtacaagtt ttacctcgat tggacacggt cgatttactc cggaagctgg
    18901 tgcacaacca gcgattggga aagtagggaa gacagaacaa gttcaagagg caaaagttga
    18961 ggttattttg ccggaaacta tcgaaaaaca agtgattcaa gccatgcggt cagctcatcc
    19021 ttatgaggaa cctgcttatg atcttttcgc tatagatgaa ccagtcgaaa tgtttgggtt
    19081 aggacgagta ggtgaattgc cgcaagaaat ttctatagag gcatttgtag aacaagtaaa
    19141 agaagcgttc caactagatg gactgagaat agtccagcca aaaaatgcaa aatcctctgt
    19201 gaagcgcatt gcgatttgcg gtggaagcgg tgaaaaattt tatccgcaag caatcgccca
    19261 aagagcagat gtctatatca cgggagatat ctattatcat acggctcatg atatgcaaag
    19321 cgctgggttg attgcaattg atcccggtca ttatattgaa tcgttatgca agcaacgatt
    19381 tatcgagaaa tttgaatctt ggaaacaaga agaaaattgg gatattaact ttttcgtttc
    19441 tgaaacaaat acaaacccat ttcaatttaa ttaaaaggag agaatactat gtacgaaaat
    19501 cttttgccac gttttttacg atatgtcaaa acagaaacac gctccgatgc aacaagcaaa
    19561 acgactccat cgactcagac acaagttgct tttgcgcaag aattgaaaaa agaattagaa
    19621 gagttaggct tagctgatgt tttctataat gaagataacg gattcgttat gggaacatta
    19681 ccaagcaatg ttgatcatga agtgcgttcc attggattta tttcccacat ggatacagca
    19741 gattttaatg cagtaggagt caatccacaa atcattgaaa actatgatgg cgagtctacg
    19801 atcgtactag atgcggaaga acgctataca ttgaatacaa aagattttcc aaatttgaaa
    19861 aattatgaag gccacacatt gatcacaaca gatggcagta cattactggg agcagatgac
    19921 aaatcaggga ttgcggaaat catgactgca atggagatct tgatcaataa tccttctatc
    19981 cctcacggta ccatcaaagt ggcttttggt cctgatgaag aaatcggtgt cggagcggat
    20041 aaattcgatg ttgagcaatt cgatgtagat tttgcttata cagtcgatgg tggtccttta
    20101 ggtgagttgc agtatgaaac gtttagtgct gctcaagcaa atattacgat ccaagggaaa
    20161 aatgtacatc cagggacggc aaaagataca atgatcaatg ctctacagtt agcaatcgat
    20221 tttcataatc agcttccagc cgatgaagtt ccagaaaaaa cggaaggata cgaaggtttc
    20281 ttccatttga tggcattgga tgggaatcct gaagaagctt ctatgagtta tatcattcgc
    20341 gatcatgatc gtgaaaaatt cgaagcacgc aaagcacaaa tcacacatat ccaagaagag
    20401 ttgaatcagc gatttgatca agaacgaatc aaagtagata tgtatgatca atattacaac
    20461 atgaaagaaa tcattgaaaa agatatgagc atcgtggaat tagcgaaaca agcaatgatc
    20521 gagcttggta tctctccagt gatcgaacca gttcgtggag gaacagatgg ttcaaaaatc
    20581 tcttacatgg gtattccgac gccaaatcta tttgccggag gagaaaatat gcacggacga
    20641 ttcgaatttg tttctttaca agtaatggaa aaagcaacag acgtgattgt aaaaattgca
    20701 gaattgaaca caaagagtta aaatcgaaaa agaacagctt atgacaaggc gttcgtccca
    20761 atgatggaat gtcatctgtc taaggctaaa gggcaggaca atggaattgg ttgtcccgcc
    20821 ctcttttttg aagagtaaat aggaggtaaa gaatggaaaa gctgctagtt ttttataaca
    20881 aaagttcagg gaaagatgag ggtgagaaat tagcacgctg gtttcaagat tatgtaaaag
    20941 aaaagcagcc agaattatat gtagaactag ctgaaactgg cccatcgatt gatgatgcgg
    21001 aattagtcaa gaaagcagaa gccatagaag ctgatacttt agtaattata ggtggagacg
    21061 gcacgattca tcatatcgtc caagcgttcc aaaaaacatt atctcaatac catgtcggat
    21121 tacttccagg tggaactgtc aacaaccttg cacgtgttct tgagattccc ctcaaaaaag
    21181 aagaagctgc tgacgtgatc ctggaaggca atgtacggaa aatagactat ggtcaagtca
    21241 atgatgaagt cattatctct acactgacaa tcggaatctt agcagataca gcagtttgga
    21301 tcagtcaaga agaaaaacag cgttacggct cgtggatctt tatcaagaag ttttttaagt
    21361 tattgctgaa gaagaaaaaa tatcatttgg atttagaaac agacgaggag cattggaaag
    21421 gaaaatccca actgttgaca gtcacgatgt ctaactccgt gggcggtttt acaaattttg
    21481 acgactcagc gaccctagat gatggaaaat ttcatgtaac gatcgtacct tccttagata
    21541 ttttccgatt tattttttat ttgcctagaa tcatgagagg caaattctat acgatccccg
    21601 gcattactta tttcacagcc aaacgcatga aaatccaagc gcaggaaaaa tctgtgggca
    21661 ctcgaacgga tggagacacg acagatgatc ttccagttga attatctgtt gtatcaaaag
    21721 gattaacagt ttattcacca aagaaagaag aaaaagctta gaaatagtag ttaacagaaa
    21781 gttggaagaa gagtaatgga tgtattgtta cacaatcctt tgtggtttta ccttttcatt
    21841 gtgaattttt acttattttg tctgatgggg tatgataaac atcaggctag aactagcggc
    21901 tggcgtgttc ctgagaataa tttgatattt gtcagcctgt taggcggtgg tttgggagga
    21961 ttgatctctc gaaaagtttt ccatcataaa actcggaaaa agaagtttac tataggattc
    22021 gctgcaggtg tacttgttga tttgattctc atttattttt tccattaagg agcaggagat
    22081 gtcgtctaaa ggaacgaaaa ctaaaatcat tctgaatctg cttttgctga ttgtgatttt
    22141 tgccattatt tattatttaa tcaatcaatc atttgcagat atctttgccg aattgctgtc
    22201 taccagtgtt gttgttttgg cagcggctgt tatattaggg acagtttacc aaatcgctga
    22261 agggcgctca atcaaagaaa ttgctgaacc tttcgcgccg aattttacaa cgaaagatgg
    22321 gttcttgacc tctagctata ttgcgtttta ccgaatcgtt tcgtttggta caggaacgct
    22381 gatctcggag atttattttt ataacaaaaa aggaatgaag ctatcccagg gagcaggggt
    22441 gacagcactc catatgatta tgtacaaact ggcagtgatc acttatgcat tagctggctt
    22501 ggtcatccaa ttctctcttt tttattctaa tggtccatca atgatctggt ttatcatcgc
    22561 tggtatcctt ttgacgatcg tgattgttgc ctttttattg gcgatgtccg tcagcttgaa
    22621 tgttcaaata ttttttgtca gcttatcgaa taagttattt aaaagtgagc gtttgcggaa
    22681 atgggtggat acatgcaatc agcagatcta ctctttaagg gaaacggtcc aaaccattat
    22741 ccaagacagg actgcgctat tgcggattta tctatggaat atgttaaaac ttgtattttg
    22801 gtatgtactt ccttatttga ttctgattga aaaccatcct aaaatagatt ttctactgac
    22861 catttcgttt atcagctttg cagtagtgct ttcaggggtt attcctacgc ctgctggtat
    22921 tggttccttt gaatttgtct atttgttatt attcagacca gttgttggaa cagttgacgc
    22981 agtttcttcg cttttactgt atcgtttcag ttcctttatc ttgccatttc tctatggatt
    23041 tgtgtatgtg attgctgaac ggcgaagagt gatcaaagaa gaaataatag aagtaaaaaa
    23101 agagaataaa gaaaatcaag ggtaggtctt ttataagatc tacccttttt atcaaatcaa
    23161 agaaaaacgt tatggataag ataattttac gtttgtttgg ctcttttttt atatacatac
    23221 aatcaatttt gtttttttaa gatttatata agtcgagaaa aaaagtcaaa aaactatgtg
    23281 tatattttga ccaaaattga ccaaagcgat ataatagttt ttgtaaagat caaagaaata
    23341 agtgatctta cctttaatta ggagggtata cctgtatgaa tattgagaaa atgactacga
    23401 cgcttcaaga agcgattgct gaagctcaac agattgcggt cactcgaaaa catcaagata
    23461 tcgacattgc ccatgtatgg aaaatctttt tacaatctaa tcattttggt cgaaatttct
    23521 atacggatgc cggattggat gtcgaatctt ttgaacatga aattgatcgt ctactggatg
    23581 agtatccagt tgtttctggt ggaaacgtcc aatatggaca aaacctcagc cagaatttct
    23641 ttcgattatt gaatgaagca gaccaaatac gtgaaagctt cggcgacgag tttctttcaa
    23701 cggaagtcgt tattttagcg ttgatgaaat taaaaaatta tccgttaact gtttatttga
    23761 ataaaaacgg attatctgaa aaagaattgc gtaaaaacat cgaagagatg agaggaggag
    23821 aaagagtgac ctcacaaaac caagaagaac aatacaaagc attagaaaaa tacggcgttg
    23881 atttagtaca gcaagtcaaa agcggaaaaa tggatccaat catcggtcgt gatgaagaaa
    23941 tccgtgatgt catccgtatt ctttcacgaa aaacaaaaaa taatcctgtt ctgataggag
    24001 aacccggtgt agggaaaacg gcaatcgttg aagggcttgc tcaacgtatc gtacgcaaag
    24061 atgtgcctga aaatttaaaa gataaaacga ttttttcatt agatatgggt gcattgatcg
    24121 ctggagcaaa attccgtggt gaatttgaag aaagactaaa agcagtcttg aaagaagtca
    24181 aaaaaagtga tggacgtatc ctcttgttca tcgatgaaat ccacaatatc gtaggggcag
    24241 gaaaaacaga aggaagtatg gatgccggaa atctgttgaa accaatgctt gctcgcggtg
    24301 aattacattt gatcggtgcc acaactttag atgagtaccg tcaatatatg gaaaaagaca
    24361 aagcgttgga acgtcgcttt caaaaggtat tagtcaaaga accaacagtg gaagatacga
    24421 tcagtattct gcgtggatta aaagaacgtt ttgagatcca ccatggtgtt aacatccatg
    24481 acaacgcatt agtagcagca gctactttat ctgaccgtta tattactgat cggttcttac
    24541 cggacaaagc aatcgatttg atcgatgaag ccagtgcaac gattcgggta gaaatgaact
    24601 ctatgccaac ggaattagat caagttacta gacgtttgat gcagttggaa atcgaagaag
    24661 ctgcactgaa aaaagaatca gatgatgcaa gtaaaaaacg cttgaaaaat ttgcaagaag
    24721 aattagctga acttcgtgaa gaagcaaatg cgatgaagat gcagtgggaa acagaaaaag
    24781 aagaagtcaa ttctgtttca gcaaaacgtg ctgaaatcga taaagcaaaa cacgaattag
    24841 aagatgcaga aaataactat gatttagaac gcgctgctgt attgcgtcat ggtacgatac
    24901 ctcaattaga aaaagaattg aaggaattag aagctaaagc aaaagacagc gagattaaga
    24961 tggttcaaga gtccgttacc gaaaatgaga ttgctcaagt cgttggcaga ttaacgggta
    25021 tccccgtaac gaaacttgta gaaggtgaac gagaaaaact aattaaattg aatgaaacac
    25081 ttcataaacg agtgattggt caagatgaag cagttgatgc ggtcagtgac gcggtaattc
    25141 gttcacgggc tggattgcaa gacccaaatc gtcctctagg ttctttcctt ttcctaggac
    25201 caactggtgt cggaaaaacc gaattagcga aagcgttagc tgagaacttg tttgactcag
    25261 aagatcatat ggtacggatc gatatgagtg aatatatgga aaaacatgct gtgtctcgtc
    25321 tagtcggagc tcctccaggc tatgtaggtt acgaagaagg tggtcaatta acggaagctg
    25381 tcagacgaaa tccttataca atcgtcttgt tggatgaaat cgaaaaagcg catcccgatg
    25441 tcttcaatat cttgttacaa gtcctagatg acggacgctt gactgattca aaagggcgag
    25501 tagtagattt caaaaatacg gttctgatca tgaccagcaa tatcggttca cagttactgc
    25561 tggaaggcgt gactgctgat ggaacgattc cagaagcagt ggctgaacaa gtcaacacgc
    25621 tattacgtgg aaacttcaaa ccagaattct tgaaccgaat cgacgacacg attttattta
    25681 caccattgag cctagacaac gtgaaaggaa tcgttgacaa aatggtggcg caattggcac
    25741 aacgtctgga acatcaagaa atcctgctga caatcagtga tgaagccaaa acgtggattg
    25801 ctgaaaatgc ttatgaacca gcatatggtg caagaccatt gaaacgattt atcactaagg
    25861 aagtagaaac accactagct aaagaaatcg ttgcaggaca tgttatgcct aaatcaaaag
    25921 taacgattac tttacttgat ggacaacttc atttcaaaac agaagagcta gaagaaatcg
    25981 tctaatcttc cattgagact ataaaagaaa aaaggggtct gacacaacta gtgtcagacc
    26041 tccttttttc aaataaacag tgttccagaa gtagtttctt cgaaaaataa gccgaaattt
    26101 cttgaagctg cccgcttctg tcacaacctc ttttattagg tatttttcac tttttctaat
    26161 ttataacttt caaagtaacg ctcatctttt ttactttgag accatgcaaa caatcccatt
    26221 aaggcagcac ctatcgtatt tgtcagcaag tcgcccatcg tatccatcaa tgctgctcgt
    26281 ccgacaaaca gtgtgccatc agaagcagca aaacgttgaa gattcatgcc taagaactgg
    26341 tcgcataaga attcccagaa ctcccaaaac acgccgcaaa gtccagcaaa cgcaaagccg
    26401 aataagagga acaaccaagg actggttttt gatatctcag catctttcat caagtaacca
    26461 atcaatccat agccaagagc tgtcaggacc attggactca ccgcatgtaa gattttgtcc
    26521 caaaaagaga tgatactgat caaatgcatc cctgtgccta aaaagacaga gatgaacaag
    26581 aaaaaccagt aaaacaaaac gattgctgct ggtattttaa gtttgaaaac tttcaaaatg
    26641 acttgtggca tgaaaatcaa tgcaatccct aataaagatt ccacgataaa tgtgattcct
    26701 tgagaagtcg aatatttttg tgtgataaat tcataaaaat tatagcagac aacgaataca
    26761 ccaaaagcaa gtaaagctat ttgcaccttt ttgatttctt taatcactga aagtccctcc
    26821 atctaaaaat ctttttactt ctgaaagcaa ctcaatttct gaagaaacca cttttccgtt
    26881 caatttgatc aaacctattg tatataagtt caggtagtga aattgatttt cagctgtttc
    26941 ttgtagtgca gcgatttttt tcgggtgggt tccgccttgc tgtcgagtat cagtatataa
    27001 gccgatcact gggatgcctt tcgcataagc cacaccgatt tccgatgcta ccccggcatc
    27061 gatcgtcagc ccatctagta aagcaatcat taaatcacta gccaacactt tttctgtgtc
    27121 agcaagagca atcattcgac tgtctgcata agcagttttg tcattgattg cttcattttc
    27181 ttgaggaaga tagatcgaaa gctcagaaga gagctctctg atttctttaa ctaactgttg
    27241 gttatataac aaatcacttt tagcaaacat tggtgcggca aaataaattt tcatctaaac
    27301 atcatccttt ttatcaaaaa taatcgctaa agtaagcttc catgtttgga atgatacgct
    27361 ctagtgactt cagtgtttct tcatctgttt ctaaccgttc gattcttgcc aaataagaag
    27421 cacggcgata attcatcatc aagcaagtca atgtttggat atccagtcga acggattttc
    27481 ctataggttc atttaagaca cggacttgtc cctgttcatc ccatgttaaa gcgaagacac
    27541 cattattcca aggagcgaca ggatcttcaa tgatgaaatg aaaagcatca gctgttccaa
    27601 caaacggaaa ccgttgaagg aattctttta catcaacgat ccgagccata aaatatggtt
    27661 cgatctgctc tttgatctga ctatcttcaa tcaaaaatgc taaaggctca tttttataaa
    27721 tatctccatg tacccagtag atcatggaaa aatgtgcgga aataaagttc cataagccat
    27781 ttcgagcttc ttgatcaaga tagaacattt cttttacgtg aaatatttct tcagctaccc
    27841 agtagaacag aacacctctt ggttcatggt tgctatcgta gtagacagcg gctgtgcgct
    27901 catcttcatt ttcgaaacgc caatactctt cccaatgaaa atcactgcgg aataaagcac
    27961 catggttttg ttgggcaaat ttgtcataaa cggaaaatac gtctggatga tcaactggta
    28021 atcgctcaac cataccactt actgggattt gtttaggtag ctgtgtatct ctgattttaa
    28081 aggaaagttt atcagacatg atttcccatc ctttacgccg atagtagggg atactgtaag
    28141 gatacaaata agagatccat tgcttattct tgcgcatatt atccaatgca acgataatta
    28201 gatcttgcat caatccatgg ttagcatatt ctggataagt tccaactcca gtcacaccac
    28261 ccattttata acgtgcaccg tgaatattga cttcacaagg atagatagcg atttgagaga
    28321 tcagtttatt ttcgtggaac catccgaaca ctttcgatac ttccaaaatc ggttttttgg
    28381 aacggataaa agctcgttta ttctcaaacc cgctttcctc gatgtctgct tcagtcactt
    28441 ggaacacata ggaaagcaat tcattgaatt gatcgatatg ttcttgagtg accggtttta
    28501 attcaagatt ttctctaaaa tctttttgat ccattttttc atctccattt attagcttga
    28561 taacagtata gcaaaattct acaaaaaaaa cttctatagc tgactaatta aaaactcttt
    28621 aaattactgc ttttcgttgg tataatggaa agtgctgatt atatgaaaag aggagacgct
    28681 atggatataa agaaaatgaa agaacgccaa gagcagattc gtaatttttc gatcatcgct
    28741 catatcgacc atgggaaatc tacattggcc gatcgtattt tagaaatgac gcacactgtg
    28801 acgtctcgcg aaatgcaaga tcagctgtta gactcgatgg atttggaacg tgaacgtgga
    28861 atcacgatta aattaaatgc tgtagaactc aattacacag cgaaaaatgg agaaacatat
    28921 atctttcact tgattgacac cccagggcac gtcgacttca cctatgaagt ctctcgaagc
    28981 cttgctgcct gtgagggtgc cgttttagtg gtcgatgcag cacaaggaat cgaagcacaa
    29041 acattggcca atgtttattt agcgttagac aatgatttgg agatcctgcc agtcatcaat
    29101 aaaatcgacc tacccgcagc agatcccgaa cgtgtccgtc aagaaatcga agacgtaatc
    29161 ggaatcgatg ctagtgaagc tgtattagcc agtgccaaat cagggattgg gattgaagat
    29221 attctcgagc agatcgtgga atatgttcct gcaccaagtg gagacttaga tgcaccgcta
    29281 aaagctttga tctttgactc tatttatgat agttaccgcg gtgttgttct aaatgtccgg
    29341 atcacggacg gtatggtcaa acccggagac aaaatcaaac tgatgagtaa tggcaaaaca
    29401 tttgatgtta cagaggtcgg ggtcttctcg ccaaaagcag ttgctagaga ctttttgatg
    29461 gtcggtgatg tcggttatat cacagcaagt atcaaaactg tccaagatac acgagtaggg
    29521 gatacagtaa cgttagcaga caatcctgcc gctgaagctt taccaggtta ccgaaaaatg
    29581 aatccgatgg tttattgtgg tctttatcca attgatactt ctcgttacaa cgatttgcgg
    29641 gaagctttag aaaaactgca attgaatgat gctgctttac aattcgaacc agaaacctct
    29701 caagcattag gttttgggtt ccgctgtgga ttcctcggac ttcttcatat ggatgtcgtc
    29761 caagaacgct tggaacggga attcaatcta gaattgatca cgactgcacc atccgttatt
    29821 taccatgtta ataaaacgga tggtacgact gctacagtag ataacccggc agattttcct
    29881 gaacctgtaa cgattcagga tgtagaagaa ccattcgtca aagcacagat catggtccca
    29941 aatgattttg tcggtgcagt aatggaactt tctcaacgaa aacggggaga attcattacg
    30001 atggattatt tagatgatta ccgagtaaat gtcgtctata atattccgct ttctgaaatt
    30061 gtctttgact ttttcgacaa attgaaatca agcacgaaag gctatgcctc attggattat
    30121 gaaatgtctg gttatcaaaa aagcaagtta gtgaagatgg acatcttgct gaatggagaa
    30181 aaagtcgatg cattaagctt tatcgttcac cgagactttg cctatgaacg tggaaaagca
    30241 atcgttgaaa aactgaaaaa actaatccca cgccaacaat ttgaggtgcc aatccaagca
    30301 gctattggac aaaaaatcgt tgcccgttca gacatcaaag ccttgcgtaa aaacgtcttg
    30361 gctaaatgtt atggtggaga tgtttctcgt aaacgtaaac tcttagagaa acaaaaagaa
    30421 gggaagaagc ggatgaaaca aatcggctcc gtcgaagttc cgcaagaagc cttcatggcc
    30481 gttctgaaaa tggacgagga cgaaccgaag aaatagcaag ggtttgtttg aaaacagtgt
    30541 aaaaaataaa ccatgtttac atggatttgc caccggtttg ccaccggtgg tttttttcgg
    30601 tggcaaaaat caagaattct ttctaagatt ttccatgata tcgaccgttt catttctcat
    30661 cttatctgtt acatgcgagt aagtatccat cgtaattgaa attctgctgt ggcccaatcg
    30721 ttcagagatt tctttcattt ttgcgccatt ttcgagaaga agtgtagcat gagtatgtct
    30781 gagagaatgg aagttaaaag agagggagag tgcattcgat atcctcctag tattccattt
    30841 cactacactt ggcgtaacta gctcaccgtc ttccttcgta catactgcat ttgaatcaat
    30901 gtagagcttt ccatacttca ttcgattttc taattgttgt ttcttatgtt ttttcagaat
    30961 tgctagcaag gtttgtccaa taaaaatcgt tcgattggaa ctgcttgtct ttggcgtacc
    31021 atatacccat gcgccgtcat tctttaccat ttgtttctct acagtaattg ttccatttga
    31081 aaaatcgaca tcatcccacg tcagaccaca aacttcgcca acgcgcattc ccgtataaaa
    31141 tccaatattc aaaggaatat agaaaggatg tccttcagga gtaatttcta gcatatgatc
    31201 aaagtcctca agagaaatga tttttagatc ttttttagtc gttggtcgtg cttcatattt
    31261 tggtattttt acatacagca taggattttg cttgattaat ccccaaggat aaaccgccat
    31321 attcagcgca ttcttaagga cagagtgagt aatagtcatt gttttcttcg agtaaccctt
    31381 tttaaattca gcattgatga aattttgtaa aagagcaggg gagagatccg taagtttttt
    31441 ctttcctaaa taaccgttta tatgattttt gatggtaaat cggtagtttt cataggtatt
    31501 gtattttaga tttagtttaa cgtattcctc catccaaaaa tcaaggtatt gttttactcg
    31561 agtatccgta cctaaaaagt attgtcctgt ttcgtcaata tctgataaaa cttttcgtaa
    31621 agcagcttcg gcctctggtc gggtgtctcc gccaactttc tccacttttt ttcttgagcc
    31681 atcatcattg atatct
//
LOCUS       DACDXO010000028        31375 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E23605_2017
            SAMN12639989-rid9881463.denovo.028, whole genome shotgun sequence.
ACCESSION   DACDXO010000028 DACDXO010000000
VERSION     DACDXO010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639989
            Sequence Read Archive: SRR10023389
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741914.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:42:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,972
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 33 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 17 of 73
            Pseudo Genes (multiple problems)  :: 10 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31375
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639989-rid9881463.denovo.028"
                     /isolate="E23605_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            339..1262
                     /gene="truB"
                     /locus_tag="IWM35_001469"
     CDS             339..1262
                     /gene="truB"
                     /locus_tag="IWM35_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HAP9919329.1"
                     /translation="MDGLLPLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEKKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVERFKRVDITDEVWQKVKNGMRLDYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            1266..2207
                     /gene="ribF"
                     /locus_tag="IWM35_001470"
     CDS             1266..2207
                     /gene="ribF"
                     /locus_tag="IWM35_001470"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HAP9919330.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGETWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
     gene            2559..3344
                     /locus_tag="IWM35_001471"
     CDS             2559..3344
                     /locus_tag="IWM35_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP9919331.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWVNNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENNKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            3357..4208
                     /locus_tag="IWM35_001472"
     CDS             3357..4208
                     /locus_tag="IWM35_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
                     /protein_id="HAP9919332.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(4332..4784)
                     /locus_tag="IWM35_001473"
     CDS             complement(4332..4784)
                     /locus_tag="IWM35_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9919333.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQFREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNYVVNFSQWINEKLLIQTDISLVS"
     gene            complement(5249..5617)
                     /locus_tag="IWM35_001474"
     CDS             complement(5249..5617)
                     /locus_tag="IWM35_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chaperone"
                     /protein_id="HAP9919334.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
     gene            5767..6249
                     /locus_tag="IWM35_001475"
     CDS             5767..6249
                     /locus_tag="IWM35_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA editing protein"
                     /protein_id="HAP9919335.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLSNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            6286..7296
                     /locus_tag="IWM35_001476"
     CDS             6286..7296
                     /locus_tag="IWM35_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HAP9919336.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPTWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQSSEKYQKVWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTIRHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
     gene            complement(7411..8124)
                     /locus_tag="IWM35_001477"
     CDS             complement(7411..8124)
                     /locus_tag="IWM35_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9919337.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIRQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLIDVTVIDN"
     gene            complement(8314..8904)
                     /locus_tag="IWM35_001478"
     CDS             complement(8314..8904)
                     /locus_tag="IWM35_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HAP9919338.1"
                     /translation="MKTAITFLLEEYADWEGAYITSTLNQNEKWQNKTASIQSEVTSI
                     GGLKTKIDYLIEQIPEKVDLFIMIGGNSWDIKNHHLYQIIDKLLKTDVPVAAICGAVD
                     YLAQSGLLQGYKHTGNAQFPWLESENYQNKEDFIEQQAVKDRNLITANGTAPIEFTDL
                     ILQAVHFETEEIREKTIRLYKLGFYDYYKKYGDPFS"
     gene            9388..9615
                     /locus_tag="IWM35_001479"
     CDS             9388..9615
                     /locus_tag="IWM35_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HAP9919339.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            9631..10797
                     /locus_tag="IWM35_001480"
     CDS             9631..10797
                     /locus_tag="IWM35_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HAP9919340.1"
                     /translation="MIETINPAENKVSAYIHIPFCEHICYYCDFNKVFLEGQPVDEYV
                     EMLLNEMKIMLESHPTANLETLYVGGGTPTSLSAKQLDRLLSGAREILPFQNGNEFTV
                     EANPGDLTREKLQVMKNYGVNRLSMGVQTFDNRLLKKIGRKHTAEDVYQTMQFLEAEN
                     FTNVSIDLIYALPGQTLEGYLDTLEKALALDLPHYSLYSLILENKTMFMNWVRQGRLE
                     LPDQETETRMFEETIEAMEKHGRHQYEISNFGLSGHESQHNLMYWNNDHYFGFGAGAS
                     GYLGDIRYRNKGPIQHYLRPLRANERPVLEEEKLTRKNQIEEEMFLGLRKKTGITKKH
                     FYDRYQKTIESVYGDVLRELEQEGLLVNEEDRVYLTPRGTFLGNNVFERFLFEK"
     gene            11031..12074
                     /gene="hrcA"
                     /locus_tag="IWM35_001481"
     CDS             11031..12074
                     /gene="hrcA"
                     /locus_tag="IWM35_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HAP9919341.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEDKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            12168..12731
                     /gene="grpE"
                     /locus_tag="IWM35_001482"
     CDS             12168..12731
                     /gene="grpE"
                     /locus_tag="IWM35_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HAP9919342.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            12782..14611
                     /gene="dnaK"
                     /locus_tag="IWM35_001483"
     CDS             12782..14611
                     /gene="dnaK"
                     /locus_tag="IWM35_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HAP9919343.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            14762..15928
                     /gene="dnaJ"
                     /locus_tag="IWM35_001484"
     CDS             14762..15928
                     /gene="dnaJ"
                     /locus_tag="IWM35_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HAP9919344.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
     gene            complement(16010..16675)
                     /locus_tag="IWM35_001485"
     CDS             complement(16010..16675)
                     /locus_tag="IWM35_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar diacid utilization regulator"
                     /protein_id="HAP9919345.1"
                     /translation="MELDFSSPYYVVVGQLSDNQQLCQERINPLLELLERVFSYYGQA
                     LLTVHRQQIIILVNRISRASLLSLLDKIQTKFNKMYQLQLNFGIGSLCYTEQETPQSF
                     LHAKQVCEWIAFHQSVNEIRFFEDLDLGIVLPAIPSDQRTLYVKRILKSLTEEEVHLF
                     KKTLACFSKNNGSIKNCSEELFIHKNTLQYRLNKFHSLTGYTPRNYDDYHILKLAFLL
                     VQT"
     gene            16651..16824
                     /locus_tag="IWM35_001486"
     CDS             16651..16824
                     /locus_tag="IWM35_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAP9919346.1"
                     /translation="MEKKNQAQLSEGGIDYVLDIINEPMVLSYAMEQVERLLVCAGKQ
                     AMRLIRLGEKIRR"
     gene            complement(16869..17606)
                     /locus_tag="IWM35_001487"
     CDS             complement(16869..17606)
                     /locus_tag="IWM35_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAP9919347.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(17608..19110)
                     /locus_tag="IWM35_001488"
     CDS             complement(17608..19110)
                     /locus_tag="IWM35_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP9919348.1"
                     /translation="MKRKHLFHSFVFLMTGLVTFAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            19369..21363
                     /gene="uvrB"
                     /locus_tag="IWM35_001489"
     CDS             19369..21363
                     /gene="uvrB"
                     /locus_tag="IWM35_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HAP9919349.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKAELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQIRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKDAAKALDFETAANVRDMILELKASK"
     gene            21373..24189
                     /gene="uvrA"
                     /locus_tag="IWM35_001490"
     CDS             21373..24189
                     /gene="uvrA"
                     /locus_tag="IWM35_001490"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HAP9919350.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQEIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            24452..25717
                     /locus_tag="IWM35_001491"
     CDS             24452..25717
                     /locus_tag="IWM35_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HAP9919351.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            25721..26452
                     /locus_tag="IWM35_001492"
     CDS             25721..26452
                     /locus_tag="IWM35_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HAP9919352.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLRPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTALQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSEALDTASEK"
     gene            26587..27471
                     /gene="rapZ"
                     /locus_tag="IWM35_001493"
     CDS             26587..27471
                     /gene="rapZ"
                     /locus_tag="IWM35_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HAP9919353.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            27468..28466
                     /locus_tag="IWM35_001494"
     CDS             27468..28466
                     /locus_tag="IWM35_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HAP9919354.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAINR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            28500..29435
                     /gene="whiA"
                     /locus_tag="IWM35_001495"
     CDS             28500..29435
                     /gene="whiA"
                     /locus_tag="IWM35_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HAP9919355.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            29978..30628
                     /locus_tag="IWM35_001496"
     CDS             29978..30628
                     /locus_tag="IWM35_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9919356.1"
                     /translation="MFIKGGQKMCFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLR
                     AGLITFISIVGFFLFTVIVFIWRTTAPPLPEITYGEFPFSLEYEIDEERILIEDTVIA
                     EFIGSFRGNVTTQPRRSWSIKLLNNSGELKAFHYEFLIIESEDVTITLSSGSAGYFMN
                     DGGSKPQLTVRDRIAGFMHFSHYDLEEARKILAEYGITLINWEYAPPIRNFFNDYY"
     gene            30815..>31375
                     /locus_tag="IWM35_001497"
     CDS             30815..>31375
                     /locus_tag="IWM35_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HAP9919357.1"
                     /translation="METVRLWIFMKNFRSALRYVILESKSGYYLIDTDHYTFWGGYLF
                     PIFNWIIPHKVMKINLPAEEINRLILNKDLQRKREKEHSNVAWIGPAFAVVTSSIVGP
                     MLESIYSDFSIGIRYLFVTLSIAVLLCIKGYVSKKNKDILTLIGVNNLSSTKIILFPE
                     SIKEGSRLLIIFIMLILFSVLFGVMFI"
BASE COUNT        10381 a         5262 c         6664 g         9068 t
ORIGIN      
        1 ttttttacat tgattaagaa actgaaaaaa taaataaaaa aaacggaaaa aagactggaa
       61 gaatggccag tctttttttt cgtttttggg aagtgaaaac caaactattt acctgtaagc
      121 cacccataaa tccaatcaaa tggtgcaaag aaaaaatctt ccattctatt ccctccttta
      181 aaaaagcata acatgatttc tatttacgtg agcatgaatt ttgttacgtt tttattgccg
      241 aaaaataaaa accgttcatt atgatgtcaa attcgtcagt aattgatgca atgtgttata
      301 ataattcagt taaatcaaaa atgaaatgga gaaaagacat ggacgggtta ttacctttat
      361 ggaaagaacg cggaatgaca agtcatgatt gcgtattcaa attaagaaaa atcttacata
      421 cgaaaaaaat cggacatggg ggtacgcttg acccagacgt agacggtgtg ttgcccattt
      481 gtattggaaa agccacaaaa gttatcgaat atttgacaga ttctgggaaa atctatgtag
      541 gcgagataac tctaggtttc agtacaacga cggaagatcg atccggagaa cgggtagaaa
      601 caaaaccagt agatatgtct ttatctgaaa aaaaaataga tgaagtgatg gcttcattta
      661 ttggtgagat cacccagata ccaccaatgt actcagcggt aaaagtcaac ggtaagcggt
      721 tatatgaata tgcccgcaat aatgaaacag tggagcgtcc tgttagaaag gctcagattt
      781 accgttttat ccggacaagt gaattgacaa gagacgaagt gacaggaacc atttcttggc
      841 gcttcgaagt agaatgcggg aaaggaacct atgtccgcac attagctgta gatacaggca
      901 agaaacttgg ttaccctgcg cacatgtctg atctgacccg aacagcaagt gccggtatgt
      961 cccagaagga tgctctgaca ttagaagaag tagctcaagc tgtgaaaaat gggacaatcg
     1021 atgattgttt gctgccaata gaaaaaggag tggaacggtt caaacgagta gatattactg
     1081 atgaagtatg gcagaaagta aaaaacggta tgagacttga ttatactgta tttgcgctcc
     1141 cagaaatgcc gtcagaagaa attgctttgt tttataaagg aaaagttgtc agtatttatc
     1201 agccaaaccc gaaagaaaaa aacaaattaa aacctagtaa agtattgaga aatgaggaat
     1261 aacgaatgaa tatcatcaag atcagacatc cttaccaatc agaacaaatt ccagaagacg
     1321 atgtcgtttt agtgctggga tttttcgacg gcgtgcatat cggacaccag aaagtcataa
     1381 agacaggtat ggaaattgca caaaaagaag gattgaaatt agcattgatg actttcaatc
     1441 agcatccatc tattgtcttt aaaaagatcg atcctagcag cgtgaaatat ttgactagcc
     1501 ttaagcaaaa agaagaaaaa atggccgctt tgggtataga ttatttatat gaagttgatt
     1561 ttacttcttc ttttgctcat ttagcaccgc aggaatttgt tgatcaatac atcgtagggt
     1621 tacatgcaaa atatgctgtt tccggttttg attatacgta tggaccaaaa gacattgcag
     1681 atgtcgctca ttttcctgaa tatgccaaag gaagattcaa aatcgtcaca gtttctaaag
     1741 aagaagaaca aggtgaaaaa atcagctcta cccgtattcg tggattacta gacagcggac
     1801 aagtaaaaga agcagcaaaa ctattaggtt gcacatatga ggtagaaggg gtagttgtcc
     1861 atggcgatgc acgaggcaga ctactaggat tccctacagc aaatgtcaaa gtaaagagca
     1921 cggttcattt gcctaaagaa ggtgtttatg tgtcggaaat aaaaattggg gaaacatggt
     1981 atccagccat gggttcaatt ggacataatg atacattcgg tgaaggacgg gagctgactg
     2041 tagagttgta tattttggac tttaatcaag atatctatgg tgaacatgtc gcggtacgct
     2101 ggaatcaatt tttaagagat caagtgaaat tttctagtgt agaggaattg attgaacaac
     2161 tcaaggagga tgaacaagca acagctgaat atttttctca gcaataaaac attatgcata
     2221 aaatctcttg tttatgtata actagcaatt acaataaaat gggtgataaa taagcccagg
     2281 aaaggggctg tgacaaaagt cttgtcacag cctttttttc caaataaatg gtttttcaga
     2341 agtagcttct tcggaaataa gccgaaattt cttgaagctg accacttctg tcacaacctc
     2401 tttctttata gaaataaaag gacactagca attcatgaaa acgattgcta gagcgtatca
     2461 tttgctttag actatatatc ttggtgtaca ctcactttaa ggcggaaata aaagttatcc
     2521 gtaataacag gtttaggtat gaaaaaggag tgggtactat gtacaaggaa ttagaaaaca
     2581 aagtcgcagt agtaactggt ggctcaaagg ggattggaac agcgattgct caacgattcg
     2641 ggaaagaaaa aatgaatgta gttgtcaact ataactcgga taaagaagga gcagaaaaag
     2701 cagtcaaagc aatcgaagag gcaggcggaa aagcagtctc tgtacaagca gatgtcagtt
     2761 ctgaagaagg aatacagcgt ttacttgata cggctttaga aaattttggg acactggacg
     2821 tttgggttaa taatgctggg atggaaaatc agcatccaac ccatgaactt agccttgaag
     2881 attgggaaag agtgttaaaa gtcaatttga ctggcgtttt tatcggaacg aaagcggctt
     2941 taaattactt tacagagaat aataaaaaag gaaatatcat caatatgtct tctgttcatg
     3001 aacagatccc atggccaacg tttgctcatt acgctgcttc aaaaggcggg gtaaaattat
     3061 ttaccgaaac ggtagcaatg gagtatgcac cgaagggaat cagaatcaat agtataggcc
     3121 ctggtgctat ccgtacacct attaatgcgg caaaatttga tgatcctgaa caaaaagaac
     3181 tcttggaaag tatgatccca ctaggacgcg taggtgaacc aaaagaaata gcggcgacag
     3241 ctgcatggtt ggcttcagac gaatccagtt atgttacagg tattacacta tttgcagatg
     3301 gtgggatgac tttatatcct tctttccaag gtggaaaagg ataagggtga ataaaaatgg
     3361 gtttattgat tgcattgatc ccggcagttg gctgggggat ccagccactg attttgaaaa
     3421 agattggtgg cagaccaaca aatgagatat taggaactgg tattggagcg gtgcttgtcg
     3481 gcttactcgt tcaattattc gcttctccag ctggtatttc agtgactaca tttttgatta
     3541 gtttactttc tggtgcattt tgggttattg gacaaacagg acagtataca tctttcaact
     3601 tgattggtgt ttctaaaacg atgccaattt cgactgcatt acagttagtc ggaacctcgt
     3661 tgattggtgt cttcgctttt ggagaatggt ctggaagtat ggggaaaatc attggagcag
     3721 cagcaatcat tcttcttgta tttggttctg ctttaacggc aatcagtgat gatcctggaa
     3781 aaaaaggcgg tatcacaaaa ggaattagtc tattagcatc aacaagtatt ggttattggg
     3841 tatacagtgc attgcctaaa ctagttgatg caagcggact ttccattttc tttccgcaaa
     3901 tgatcggtgt ctttttaggg gcagtgattt atgtgatttt ccgtaaacca accgcatttt
     3961 ctgaagacaa gagttggaaa gcaacgatcg ttggagccat ctttagtgtg tcagcattgg
     4021 cttatatttt ttcagcaaat gaaaatgggg ttgctactgc atatatcatt acacagctaa
     4081 acgtagttat ctctacatta ggcggtatgt acatcctgca tgaaaataag acaccaaaag
     4141 aaaaaaaatt aacgattatc ggattattgc tgatcgtagt aggaagcatg gtcacggtct
     4201 ttatttgagg ataaagatgg taaaatttca tttattaaaa aaatatttgt ttgaaccaaa
     4261 cagtaagtac ttataaaaaa atagcgctag aatttccaac atctctggaa tttctagcgc
     4321 tttttaattg tttaagatac aagggaaata tctgtctgga tcaataattt ttcattgatc
     4381 cattgactaa agtttacgac ataattgaag ttaccaccga ttagtatctc cgcatgattg
     4441 tctttataaa gatcgtattg taaaagacgg tcttttttat ttgcgtaaac atcgttatgg
     4501 tactggacta attgttctcg gaattgattg attgctgtaa aatcacgata ttgaaggttg
     4561 aaacgcatcg tgattggtgc atttagcatg aatcgctgca tttcttttgc tgcttcctct
     4621 gcttgatctc cgtataggaa ctcaaaaaga ttacgttcag aaaatttcca ttgcccgcca
     4681 acctttgttc cttttagttt tccttcatga agataatttc tgatagaacg tgtagtaaca
     4741 cctaattgtt cggctaattc atttactgag taaaaatctt tcaatgttca ccctcctatg
     4801 tgaatggttc tactgactta gttgatattt gcttaatcac gaaatgtaga aagaacgatt
     4861 ttaattaagg tcattgtaaa aatcaagtca gaaaattcta acttttattt ctctaaagaa
     4921 attcgttctc tcctaaagtg atattatatt taaatatacc tgaaaataat gggcagtatt
     4981 atgaaagatc actgatattt tctcgtattt tttccttatt caaaagtttt gcaggaaaat
     5041 tgctgaaaaa ataattttac tatgtagaaa attcaaagcc attcaatcag tgattttttt
     5101 cagcacaaga aagactcctt ataaaaatag cagatacttt gtcaattaag tgtttaacca
     5161 gggatgataa aaaggatagg attgaacaaa acctgtataa ggtgatgaat agtttcctcg
     5221 ccagtaaaag gtttattcgt ataaagcttt atcgccaaaa tttatcatga ccttttttat
     5281 cttcaatatt aaatgaaatc aattgttcaa tcaattccca gttgattaca cttttccaag
     5341 gaactttgat gacgttatcc gtatatacgt agcctgcttc ttggatattt ttttcaaggg
     5401 cacgaatcgc tgctttttcc ggtgcgatag aaaaatgatt tttagcaaaa ctaaatgcaa
     5461 taataaaggt accatggtca gtaaacatag gttggttcca ttttacagtt gtgtctagct
     5521 ggggatacgt ttctgttatc ttagcaaaaa ggtctttgat cttttgttga tgttcttttt
     5581 ctttgatttg attgatgatc ggctgaaatt tttccattct aaaacgcctc cattaagtgg
     5641 tttgatttat tttaccagaa aaacaatagt tatgtataaa aaacgaacaa aacacaagta
     5701 atgttgtaac cgctatattt atatgataag atgtctcaaa taataaaata gaaaagagac
     5761 ataaaaatga ttgacttggc aaagaaattc ttacacgaaa atatatattt tgagttgtat
     5821 catcatcgag ccatttatac aaatgaagat gctttggtaa taaagaaaga acaaggattt
     5881 acaggtacag agaccaaaag cttattctta aaagataaaa aagataataa ctacgtatat
     5941 ctgactttta ctacgaaaca aacggatttc aaagcattag ggaagatcgt cggtaaaagg
     6001 ctttctattg tttcatcaga acgcatggaa gaacaaacgg gacaaaagca gggcgctgtt
     6061 tctccgtttg gttatgaaaa acagattccg attattattg atgaagagct cttatcaaat
     6121 gaaaaacttg tttttgcacc aggtagacca gatcaaacga tggtcatcca aacggttgac
     6181 ttaaacaaaa tcatccagct acttgagatg ccgatatttt tgatgccggc aaacgaaaaa
     6241 aacaattaac caagtaaaat agtgcatact tggaggtgaa gcaacatgat aagttatcca
     6301 ttattagaaa gaaatgcaac gattggggtt actgcaccat cctcaggcgt atcagaggaa
     6361 caccattcgt tactcgaatc agctttaaag agaatggaag aaaaatcttt ccaagtagtt
     6421 actggaaaga cgctttggac gcaagataag gaaaaatcag gtccagcaag aaaacgagca
     6481 gatgaattca atcaaatgat gcaagatgaa aatatccaaa tgatctttcc gacttgggga
     6541 ggagaattat tgattgagat tctcgatttt gtcgattttg atgctctaca accaaaatgg
     6601 atagtaggtt attcagatat tagtacgttg ctacttgctg tcacattaaa aacagggata
     6661 gccactgcac atggaactaa ccttattgat ttaagaggag agtacttgga tgaaacgaca
     6721 gcaatgtggg aaagtgtact gaaaacaaga gaaggagaat cgattctaca gcaatcttct
     6781 gaaaagtatc aaaaagtgtg gccccatgag cattcatcat cttctgtttt ccatctcact
     6841 gaaccaacaa attggcaatc agtcagcgga gatgcagtaa ggatgaaggg acgattatta
     6901 ggtggttgta ttgatacgat caggcattta atcggtactc cttatggaga tgtccaatct
     6961 tttaaaagaa agtataatga tggaaagccg atcttgtggt ttctagaaaa ctgtgagcta
     7021 tcgtcagttg atttaagaag atcacttgtt cagatgaaac tggcaggctg gtttgatggc
     7081 agctcgggga tcatgttcgg tagaagtgcg gcagaggatt tgttagatta taaaatgaag
     7141 gatgtatacg aagatctgtc aaaggaatta aaactaccga tcttgtatga tattgattgt
     7201 ggtcatgtac cgccacaact gacattgatt aatggtgcct atgcagaagt agcggtagaa
     7261 gatagaaaag cgactgtttt gcaaacgttt atttaaaaaa ttttttcctg ccttggagtc
     7321 tatcaaagac caataaatct aaatagaaaa gaaagtgaaa gaagctgaca aaatgctgtc
     7381 agcttctttt tgtataacat attagaaagt ctaattatct ataactgtca catcgattaa
     7441 ctgtttcacg tcattgggat ttcctgtagg aatagctact acagatgcat tatcaatgat
     7501 ttcttgagtt gtttctctaa tgaacgctgg ataagatgtt tgatctgcag gcatttgcca
     7561 atgtgaagga actggataac tagtgattgt cccgtcatgg ttacttgaat acactaaaac
     7621 tccctggtaa ctgtatttcc cagtgagcat aaccacatct ttcggataga ctgcactccc
     7681 tgcatcataa ggggcgatag gtgttcctgc aggataatga ataacatgta attcgaaatc
     7741 acccgatgta tctctaaaag tattacctct aatagctagc caaacacgtg cgtactcaat
     7801 ttgtagatca gaatatcctg ctaacggatc tattgactga gaagactgtg aggaggttgt
     7861 gtctattgtt gaagaagaaa tatttgtctc aactgtacta ctagatgtag aagaagtatt
     7921 gggctctgag actttactgc ttgttgaaga tgtattcgtt tcagtagaag ctgatttctg
     7981 agtacttgat ttttcagctg aactggtttg tctgattgaa cttttgtctg tggtcgcaga
     8041 tggatcacta ttttctttac taggagacgt acatcctaca aggaataaag agaccaacaa
     8101 taaaataaaa cctaactttt tcatataaaa tacctcatta tttttttaat accagtatag
     8161 cataatcctg aatttttgaa tccgtaaaac cttgtctatg aatataaata gagtgtatct
     8221 tctttgataa atgtcagaaa aagtgtgaca aataatcatc agtaaaatta cttgtcacac
     8281 tattgataag gagtatttaa ttgaagagaa tgattaggag aaaggatctc cgtacttttt
     8341 atagtaatca tagaatccaa gtttgtatag acgaatcgtt ttttctctta tttcttctgt
     8401 ttcaaaatgg actgcttgaa gaatcaagtc tgtgaattct ataggagctg ttccattcgc
     8461 agtaatcaaa ttacgatctt tcacagcttg ttgttctata aaatcttctt tattctgata
     8521 attttccgat tctaaccaag gaaattgcgc atttccggtg tgtttgtaac cttgcaataa
     8581 tccgctctga gctaaataat caactgcccc acaaatagca gctactggta cgtctgtttt
     8641 taacaattta tcgattattt gatataaatg atgatttttt atgtcccatg aattgccacc
     8701 gatcataata aaaaggtcaa ctttttcagg tatttgttcg attaagtaat cgatttttgt
     8761 ttttaatcct ccaatggaag tgacctctga ttggatagaa gcagttttgt tttgccattt
     8821 ctcattttgg tttagtgtgc tagtaatata agcaccttcc caatcggcat attcctctaa
     8881 tagaaacgta atcgcagttt tcattttcaa gctctccttt actatctata acaaggacaa
     8941 cagttttaac ctacagacat tcattaaaat tgggtagcga cagcgaaaac aacaattctt
     9001 aagttttaga cagagtatac agatagtcaa cttaattaaa tacgaactaa ataaaaatga
     9061 caggtaatca tttttgttta ccagcattat aacattttgc atacatggat gacaaaagaa
     9121 ccaattccca cttgcttgag tacaatatta aaagaaactg gaaggcaaaa agtaattata
     9181 gaaacgaaaa ataaatcata tcgggcactc agacatgaaa acaaccctag agatatataa
     9241 ctaggtaacc agtttaatga aagaaaaaat catccaagaa gtcgattctt ggatttttta
     9301 gtggatttgt tcgccaaata cgtttagtat gcgtatatga gaggttgtaa gcaaagcgtt
     9361 gtggtctgag cacttaagga ggaatctgtg aaaatagctt ttcatatttt tgcagatttt
     9421 gacttaatgc cgaaggtcag ctttgcgatt accgtttatc gagaggctgt aaaagcaccg
     9481 tgtagctccg atgacaataa gcaagcattt ttagagctag gtttccttga ttctaaatat
     9541 gcggcttatt tcaatgagtt ggtgcttgaa cgcgatttta agaaggtcga aaacaggcac
     9601 gacttttcga cgtaaggaga aagaatttca atgatagaaa caataaatcc cgcagaaaac
     9661 aaggtttctg cgtacattca tatcccattt tgcgagcata tctgctatta ctgtgatttt
     9721 aataaagttt ttttagaagg gcagccagta gatgagtatg tggaaatgct gctgaatgaa
     9781 atgaaaataa tgctagaaag tcatccgaca gcgaatttag aaacacttta cgtagggggc
     9841 gggacaccaa cttctttatc tgctaaacaa cttgatcgac tgttatcagg tgctagagag
     9901 atcctgcctt ttcaaaatgg taatgagttt actgtagaag cgaatccagg tgatttgacc
     9961 cgtgaaaaac ttcaagtaat gaaaaactat ggcgtcaatc gtttatccat gggagtgcag
    10021 acatttgata atcgcttatt gaaaaaaatt ggacgtaaac acaccgcaga agatgtgtat
    10081 caaactatgc aatttttaga agcagaaaat tttaccaatg taagcatcga tttgatttat
    10141 gcattaccgg gacaaacact agaaggatat ctggatactt tggaaaaagc tttggctttg
    10201 gatctgcctc actattcttt gtactcattg atcctcgaaa acaaaacaat gttcatgaac
    10261 tgggtacgac aaggaagact cgaactgcct gatcaggaaa cggaaaccag aatgtttgaa
    10321 gagacgattg aagcaatgga aaagcatgga cgtcaccaat atgaaataag taactttggg
    10381 ctctccggac atgaatctca acataacctc atgtactgga acaatgacca ctattttggg
    10441 tttggagctg gtgctagcgg ttatcttgga gatattcgtt accgcaacaa aggaccaatc
    10501 caacactatt tacgcccatt acgagcaaat gaacgcccgg tattggaaga agaaaaattg
    10561 acacgaaaaa atcaaataga agaagaaatg ttcttaggac taagaaaaaa aacagggatt
    10621 actaaaaaac atttctatga tcgctatcaa aaaacaattg aatcggtgta cggtgatgtg
    10681 ctaagggaat tagagcaaga agggttactt gttaatgaag aagaccgtgt ttatttgaca
    10741 ccaagaggga cctttttggg gaacaatgtg tttgagcgct ttttgtttga aaaatagttt
    10801 aaagaatatt tgttttttat ctgaatttaa ataaaaaaag ttcaaaaata caataaagga
    10861 ctattgaaac gttgatataa ctggatttac atcaattatt tagaattagc actcttgtgt
    10921 ttagagtgct aaaaaatgga gtctttctct tgacaggaga agggcttctt gctatattta
    10981 taagtggatt agcacttaga atatatgagt gctaatgaga ggtgaggaag atgttaacaa
    11041 agcgacaaaa cgacatttta cgtctgatca tccaacacta tacgagtagt ggcgtgcccg
    11101 ttggttccaa aacattgatg tctgagggag tcaaggccag ctcagcaacg attcgcaatg
    11161 atatgaaagc attagaagat gaaggattgc tgttgaagac tcactcttct tctgggcgga
    11221 ttccatctgc tttaggctat cgttattacg tggatcattt gttaaaacct gctcgcgtag
    11281 cagaagatga tttgctgctg attcgtcagt ctctaggcag agaatttcat gaaatcaatg
    11341 agattattaa gcaatctgct gagatattat ctgagctgac aagctatacg actttttcat
    11401 tgggacctga aataaaagac cgccgactga ctggctttcg tatcgtaccc ttgaataatc
    11461 gacagattat tgctatcgtc gtaacagata tggggaatgt agaaagccaa gtgttcactc
    11521 ttcccgaata tatgggaagt caggaactag aaaaaatggt acagattgta aacgaccgtc
    11581 tagtcggaga tcctctgatg acggtgtacc ataaacttcg tacggagatt ccgatgattc
    11641 ttcataaata tttccaaacg acagaaggga tgtctcagtt atttgacacc gttcttggtc
    11701 atgtttttga agataaggtt tatgtcagtg gacaaatgaa tctcttggat tatgagcccc
    11761 gtcaagatct tgatcagttc aaatcgatgt tttcttttat gaaagactca gacggactta
    11821 cacaaatgat cactccgatg gatagcaata ttcatatcaa gattggtgct gaattaggca
    11881 atgatttatt gcagaatatg agtatgattc aagcaagtta cgagatagat ggtcatggaa
    11941 gaggtacaat agccttgtta gggcctacta gtatgcctta ttctcggatg ttcggtctag
    12001 tcgatgttta tcgaagagaa ctagcagcta aacttgctga ttactaccgt tcgcttgact
    12061 tgtcaaactc gtaatgtgaa taacgaagct atttgcagct gatcgttctc tctttcttag
    12121 taagagcaat caaacagctg actgaataat gaaaggaaga ctgtatcgtg aaagaaaatc
    12181 aagaagaact agataaagaa atgacagatg ctcagccaga accggaaatc gatgtcgaag
    12241 ctgcagaaga ttcgggtatt tcagaagctg aagcggaaga attcgaaaca gcgaaattaa
    12301 aagctgagtt agaagaaatg gaagataaat acttgcgggc tcgtgctgaa atcgcaaata
    12361 tggcaaatcg aggaaaaaat gagcgtgagc aattacaaaa ataccgttca caagatttag
    12421 ccaaaaaatt acttccatca attgacaacc tggaacgtgc attagccaca gaagtgtcag
    12481 atgatcaagg ggcaggtttg aaaaaaggcg tggaaatggt cttggaaagt ttgcgtaacg
    12541 cactggaaga agaaggaatt gaaaaaatcc ctgctaaagg agaagccttt gatcctaatc
    12601 ttcatcaagc agtacaaact gttccagcga ctgaagatac accagccgat acgattgtag
    12661 aagtactaca agaaggctac aaattacatg atcgcgtgtt aagaccaacc atggtcattg
    12721 ttgcacaata attgtgcgga tataaaactt acactttcaa ttaaagaagg agacgttaat
    12781 tatgagtaaa attatcggta ttgacttagg tacgactaac tctgcagtag cagtattgga
    12841 aggaaacgaa gcaaaaatca tcgctaaccc agaaggaaac cgtacaacac cttctgttgt
    12901 atcattcaaa aatggagaaa tccaagtagg ggaagttgca aaacgtcaag ctgtgacaaa
    12961 cccaaatacg atttcatcaa tcaaacgcca tatcggtgaa gcaggttaca aagtagatgt
    13021 agaaggtaaa tcatatacac cacaagaaat ctcagcaatg atccttcaat atatcaaagg
    13081 attcgcagaa gactatttag gtgaaaaagt cgaaaaagca gttatcactg tgccagctta
    13141 ctttaatgat gcacaacgtc aagcaacaaa agatgctggt aagatcgctg gtctagaagt
    13201 agaacgtatc gtcaatgaac caactgcagc tgctcttgcc tatggtttag ataaaacaga
    13261 tcgcgacgaa aaaatcttag tattcgacct tggtggcggt acttttgacg tatctatcct
    13321 tgaactagga gacggtgtct ttgatgtatt gtcaacagct ggcgacaacc atctaggtgg
    13381 ggacgacttt gataacaaaa tcatcgacta catggtagca gaattcaaaa aagaaaacag
    13441 cattgacttg tctcaagaca aaatggcttt acaacgcttg aaagacgctg ctgaaaaagc
    13501 gaaaaaagat ttatctggcg tatcaagcac acaaatcagc ttgccattta tcacagcagg
    13561 agaatctgga ccattgcatt tggaaatgac attgacacgt gcaaaatttg atgaattaac
    13621 agctgactta gtagaacgta caaaagtacc agtacgtcaa gcgctaaaag atgcaggttt
    13681 atcacaatca gaaatcgacg aagttatttt agtcggtgga tctactcgta tccctgcagt
    13741 tgtagaagca gtacgtaaag aaacaggaaa agaaccaaac aaatcagtta accctgatga
    13801 agtagttgct atgggtgctg ctatccaagg tggcgtgatc actggtgatg tgaaagatgt
    13861 tgttttactt gacgttacac cattgtcatt aggtatcgaa acaatgggtg gagtgttcac
    13921 taaattgatt gaccgcaata caacgattcc aacaagtaaa tcacaagtat tctcaactgc
    13981 ggcagataat caaccagcag tagacatcca tgtattacaa ggtgaacgcc caatggctgc
    14041 tgacaacaaa actctaggaa gattccaatt gacagatatt ccagcagctc ctcgtggtat
    14101 tccacaaatc gaagtaacat tcgatattga taaaaacggg attgtaaatg tatctgcgaa
    14161 agatctaggt acacaaaaag aacaaaaaat cacgatcaaa tcttcttcag gtctaacaga
    14221 cgaagaaatc gaacgtatgg taaaagatgc agaagcaaat gcagaagcag ataaagctcg
    14281 taaagaggaa gttgacctac gtaatgatgt agatgcattg ctattctctg tggacaaaac
    14341 attgaaagaa ttagaaggca aagtggacga agaagaagtg aaaaaagcag aaacagctcg
    14401 cgatgaattg aaagccgctg ttgaagcaaa caacatcgaa gaaatgaaaa ctaaacgcga
    14461 tgcattgaac gaaatcgttc aaaacttgac tgttaaacta tacgaacaag cggcacaaca
    14521 acaagctcaa gaaaacccag aagcagctca aggcggagca gatgatgtcg ttgatgctga
    14581 ttttgaagaa gtcgatggcg acgataaata atcagttcac tgaatttcga gagaaggcca
    14641 aagttgctac tttggctttt tcttttgaaa aatagtttga agtatcggtt tttgtttcaa
    14701 acaaaatatg atatgataac aagcgacgtg aaaatgatga gaaccaatag gaggaaagcc
    14761 tatggcaaca aaacgtgatt attatgaagt cttagggctc tcaaaaggag catcagaaga
    14821 tgaaataaag aaagcttatc gtaagctttc gaaaaaatac catcctgaca tcaataaaga
    14881 acctgatgcg gaagaaaaat tcaaagaagt atcagaagca tatgagattt taagtgaccc
    14941 gcaaaaacgc gctgcatatg accaatacgg gcatgcaggc acagatccaa attacggtgc
    15001 tggtggagct ggtggcggat ttggtggatt cggcggcttc tctggtggcg gcttcggtgg
    15061 ttttgaagat atcttcgact ctttctttgg aggaggcggt cggtcagtcg atccaaatgc
    15121 gcctcgtcaa ggtgctgatt tgcaatatac ggtcgacttg tcttttgaag aagcgatttt
    15181 tggtgtagaa aaagaaatca agtataatcg ggaagaaatc tgtcatactt gtggtgggaa
    15241 cggtgcaaaa ccaggcacac aaccaactac ttgtcataaa tgtcatggtg caggtacgat
    15301 taatgtagaa cgccaaacac cacttggacg agtgatgagc cgtcaaactt gtgatgtttg
    15361 tcatgggaca gggaaagaaa tcaaagaacc ttgtccaacc tgtcatggct caggacatga
    15421 gaagaaagca cattcagtta aagtgaatgt accagctggt gtagaagaag gacaacaaat
    15481 gcgtttagct ggtcaaggag aagctggaga aaacggcgga ccatttggtg atttgtatgt
    15541 ggtcttccgt gtagaagaaa gtgatatctt tgatcgtgaa ggttctgaaa tttactatga
    15601 attgccttta agttttgtac aagcagcttt gggggatgaa gtccaagtac caactgtcca
    15661 tggagatgtg aaattgaaga tccctgctgg aacacagaca ggtacgaact tccgtttacg
    15721 tggaaaaggc gctccgaagt tacgtggtgg tgcaacaggc gaccaacatg tcaaagtaaa
    15781 actgatcaca ccgaaaaatc taaatgaaca acaaagagaa gcattacgtg cattcgctga
    15841 agctggcggc atcaaagttg aagaacagca agaagacggt ttcttcgata agtttaaaga
    15901 tgcttttggc gggaaaaagc ggaagtagtt tttctagtaa aaacgctaag tcattaataa
    15961 ttaaaaacca gacgaacaat catatgttcg cctggttttt ttgtagttgt catgtttgta
    16021 ccaacaaaaa agccagcttt agaatatgat aatcgtcata gtttctaggg gtataaccag
    16081 tgagagaatg gaacttgttc aatcgatatt gcaaggtgtt tttatgaata aataattctt
    16141 ctgagcaatt tttgatgctt ccgttatttt tactgaagca tgccaaagtc ttcttgaaca
    16201 aatgtacttc ttcttctgtc aggcttttca aaatacgttt tacatataat gttcgttgat
    16261 ctgacgggat tgctggtaaa acgatcccga ggtctaaatc ttcaaaaaag cggatctcgt
    16321 tcacagattg atggaaagct atccattcgc aaacttgttt ggcatgaagg aaagattgcg
    16381 gtgtctcttg ctctgtataa caaagcgacc caattccaaa gtttagctgt aactgataca
    16441 ttttattaaa ctttgtctgg attttatcaa gcaatgatag caatgaggct ctggaaatcc
    16501 gattgactag gataatgatt tgttgtctat gtacagtgag caaagcctgt ccgtaataag
    16561 agaagaccct ttcgagcaac tccaataaag gattgatccg ttcttgacac aattgctgat
    16621 tgtcagaaag ttgtcccaca actacataat atggagaaga aaaatcaagc tcaattaagt
    16681 gagggtggta tcgactatgt acttgatatc atcaatgaac cgatggtact ttcttatgcg
    16741 atggaacagg tagagcgatt gcttgtttgt gcaggaaaac aagcgatgcg gctgatacga
    16801 ttaggtgaga aaataagaag ataaaaatac acggtccctt catggcgacc gtgcattttt
    16861 ggtttttatt aaatatttaa tactttattc aagaaatctt ttgtccgttc attttgcgga
    16921 ttttcaaaga tttgttgagg cgttccatct tctaaaaagt ttccgtcgtc aatgaacatg
    16981 acgcgattgg ccacttcttt agcgaacccc atttcgtgcg taacgataac catcgtcata
    17041 ccttgttctg ctaacttttt catgacgttc aagacatcac cgaccatttc aggatcgagt
    17101 gcagaagtag gctcatcaaa aagcatgatg tcagggttca tcgctagagc acgagcaata
    17161 gccacacgct gtttttgtcc accagaaagc atttcaggat acatatcttt tttatcttct
    17221 agaccaactg tctgcagtaa tttgattgct tttttctctg cttcttctct agattctttc
    17281 ttcaggtcca tcggtgctaa aatgatgttt tccaaaatag aaagatgagg gaataggtta
    17341 aagtgttgga ataccattcc gatatgttgt ctcaccaagt tgatatctgt gcttttatcc
    17401 atcaagtggg cgccgtcaat aataatttca ccgtcagaag gttcttctaa acgattcaag
    17461 caacgcaaga atgtactttt tcctgaacct gaagggccaa tgatacagac gacatctcct
    17521 tcgttgatgg atacattgat gtcgtttaat actgtgttat cgccgtattt tttgactaaa
    17581 tgctcaacta agattttttc tgccatctta cttcaccttc ttttctaaaa cttttgctaa
    17641 ttttgtcaat gctgtgatca aaatcaagta catgatcgca atgatcaagt aaacatacgt
    17701 actttgtgtt gtacgtgcaa caatgatctt accagtttgc agcaattcga ctacaccgat
    17761 tgcggaaatg atcgttgtgt cttttaatga gataacgaac tgattgacga atgatgggat
    17821 catgatgcgt atcgcttgcg gcaaaatgat tttttgcatt gtccgattat aagaaagacc
    17881 taaacttctg gaagcttcca tttgtccgac aggaacggcg ttgatcccgc ctcgaacaat
    17941 ctctgcgata taagcacttg cgttaagagt caaggtgata acaccagcaa ggaagtcagg
    18001 aatcgtaaat ccaacgatac caggcaaccc aaagaaaata aagaacgcaa gaaccatcat
    18061 tggaattcct cggatgatat cgacataaat actagcaatt gtgcgaagag cgcgtattgg
    18121 tgctacactg aataatccaa aaatcacacc gattactata gccaaagcaa aggagatcaa
    18181 agttaggaga attgttctcc aaagtccttt caacaatgct tgatagttgt tttggataag
    18241 tccaagaaat gttgattcat cagcagtgga agactcactg gaagtttctg aaccatccgc
    18301 tacatattct ttaataatct tatcgtattc gcctgttctt tttaattctt tcaatccttc
    18361 attgaacata ttaagaagct caggattttg tcctttttta acggcaaatg catattttcc
    18421 gccagattct tttttgatcg gtgtttccaa ttcttgtcct tgtttgatcg cataaccgat
    18481 taccggataa tcatccatca ttgcatcgat tgcgccaact tttaacccgt cgtacaattg
    18541 atcagcagca tcaaaatatt tgatagaata gccatattca gattcatggt cgcttaaaaa
    18601 ttcagcactt tcggtaccaa cttttgctcc gactgttttt cctttcaaat cttcataaga
    18661 cttgatcgaa gtatctcctt ttttgacagc taattggatc ccgctatcaa agtaagaatc
    18721 agaaaaatca tagcttttct ttcgttcatc tgttacagtc attccggcaa tcattccgtc
    18781 tgcttgtcct gattccaaag cttgcaaagc agcagagaaa ccacggtagt ccattcggat
    18841 gttaaatcct tgcagtttgg cgatgttttc catcaatttt acatcgatgc cttcgtactc
    18901 gccctttgcg ttttgaaatt caaatggagc aaaagcagag tcactggcaa ttacgtatac
    18961 gtcttttttg ggctctattt tttgcattgt ctcgcttgtg cttgactctg ccgttgtagt
    19021 gttgtcagct tgtgcaacat gaccaaacgc aaatgtcaca agtcctgtca ttaagaaaac
    19081 aaatgagtga aataaatgct ttcttttcat aatcaacctc ctaaatttcc aatattttat
    19141 tattataggc gaaaagtgct gaatcagaca agactgtaag ctcgaaaaaa tgaaaaaaat
    19201 gcgagtttct aacattttat gttataaaac ataacatgta taataagata aaaaagtggc
    19261 gaaagtatgt tcggtgtggt ataatttttt agtaaggaat gcctttgctt tccttttctt
    19321 tttattggtc gtaaaatgaa aaggaatgaa tgaaaaggag gacgaagaat gattgagaga
    19381 gatacctccc gacactttga gcttgtctcg aaataccaac cggctggtga tcagcctgaa
    19441 gcgattaacc aattagtgga tggcgtagtc ggcgggaaaa aagcacaaat cttattaggt
    19501 gcgactggaa cagggaaaac atatacgatt tccaatctta ttgagaaagt gaataaacca
    19561 acactgatta ttgcccataa caaaacattg gctggtcagc tgtacgggga attcaaggaa
    19621 tttttcccta acaatgctgt tgaatatttt gtttcttatt atgattatta tcagcctgaa
    19681 gcttatgtgc cttcaagcga tacgtatatc gaaaaagatt caagtgtaaa tgatgagatc
    19741 gacaaattac gacattcggc tactagttca ttattagaaa gaaatgatgt gatcgtcatt
    19801 gcatccgttt catgtatttt tggattaggc tcgccttttg agtaccaaaa acaagtagtt
    19861 tcgattcgac agggagcaga actagatcgc aatcagttga ttcgtgactt agtcagtatc
    19921 caattcgaaa gaaatgatat agattttcag cgcggtcgtt ttcgagtgcg cggtgatgtc
    19981 gtggagattt ttccggcttc aagagatgaa cgtgccttac gtgtggagtt ttttggagat
    20041 gaggtcgaac gtatccgaga agtcaatgca ctgacaggtg aagttttagg agaaacggaa
    20101 cacgtagcaa tcttcccggc gactcacttt gtgacgaatg atgaacatat ggagcatgcc
    20161 gtagcgaata tcaaagcgga attagaacag cgtttaactg tcttgcgaaa tgaaaacaaa
    20221 cttctggaag ctcaacgttt agaacagcga accaattacg atattgaaat gatgttagaa
    20281 atgggctaca cgtcgggaat tgaaaattat tctcggcata tggacggacg taaagaaggt
    20341 gaaccgcctt atacgctatt ggatttcttt cctgaagatt ttttgatcgt ggcggacgaa
    20401 tcgcacgtaa ccatgcctca aatcagagga atgtataatg gtgaccgagc gagaaaacag
    20461 atgctagtag attacggttt ccgtttgcca tctgctttag acaatcggcc acttcggtta
    20521 gaagagtttg aaaaacatgt caatcagatc atttatgtct ctgcgacgcc aggaccatat
    20581 gaacatgaac aaacagatac cgttatccaa caaatcatcc gtccgacagg gctgcttgac
    20641 ccagtgatcg aagttcgtcc aatcatggga caaatcgatg atctagtagg cgaaataaat
    20701 gaacgagtcg aaaaagacca gcgggtgttt gtaacgacat tgacgaaaaa aatggcagaa
    20761 gacctcacag attatttcaa agaattaggg atcaaagtca aatatttgca tagtgatatc
    20821 aaaacgctgg aacgaacaga aatcattcga gatcttcgat taggtgaatt tgatgtgtta
    20881 gttggaatca atttgcttcg tgaaggatta gacgtacctg aagtttccct tgttgctatc
    20941 ttggatgcgg ataaagaagg ctttttacgt agtgaacgtt ccttagtcca aacgattgga
    21001 cgagctgctc ggaatgaaga aggtaaagta atcatgtacg cagacaaagt aacagattct
    21061 atgcgtctag cgatggatga aacgtcaaga cgtcgtacga ttcagcagaa gtacaatgaa
    21121 gaacatggga tcgttccaaa aacgatcatt aaagaaatcc gagatttgat ttctattacg
    21181 aaagaatcgg aagatgatac aaaagaagcg gtacaagtct cgtacgaaga aatgacgaag
    21241 gaagaaaaag acacgttatt gatgaaatta gaaaaagaaa tgaaagatgc ggcaaaagct
    21301 ttggattttg aaacagccgc gaatgtgcga gatatgattt tagaattaaa agcttcaaag
    21361 taggaggaca acatggctaa cgataaaatt acgattcatg gtgcacgtgc ccataacttg
    21421 aaaaatatag atgtaacgat tccacgagat aaattcgtgg tcgttacagg tttgtccggt
    21481 tctggaaaaa gttccctagc atttgatacg ctgtacgctg aagggcagcg gagatacgta
    21541 gagagccttt ctgcttatgc tcgtcagttt ttaggacaaa tggataagcc ggacgtagat
    21601 agcatcgacg gattgagtcc ggcgatttct attgatcaga aaacaacaag taaaaatcct
    21661 cggtcgacag taggtactgt gacggaaatc aatgactatt tacgcttgtt gtttgcacgt
    21721 gtaggacatc cgatttgtcc caacgatcat atcgagatta ccagtcaatc agtagatcaa
    21781 atggcagatc aagtaatggc tttgcctgag cgcacaaaga tccaagtgtt ggcaccgatc
    21841 gtggtaaaga aaaaaggaca acataaaaaa atattcgaac gaatccaaaa agagggatac
    21901 gtccgcgttc gagtcgatgg tgagatctat gatttaagtg aagtgcctga gctggagaaa
    21961 aacaaaaaac atgatatcgc gatcgtcatc gaccggatca ttgtgaaaga agggattcgt
    22021 tctcgcctat ttgattcttt agaggcggcc ctgcgattag cagacggcta tgctctggtt
    22081 gacgtgatcg gtgaagaaga aatgttgttt agcgaacact atgcctgtcc ttattgcgga
    22141 tttactgttg gcgaactaga accaagactg ttctcattca atgcgccttt tggagcttgt
    22201 ccagactgtg atggtttagg tgtcaaatta gaagtcgatc aagatttagt gattccagat
    22261 agaacgaaga cacttagtca aggtgctatc gttccatgga atccaatcag ctctcagtat
    22321 tatcctcaaa tgttagcgca ggcttgtgaa agtttcggta tcgatttaga tacaccattc
    22381 gaagaattac cggaagagca tcaggagatc atcttgaatg ggacagatga tcttttccat
    22441 ttccattatg aaaatgattt tggtggcgtt cgcgatgtag aaacgacttt cgaaggggta
    22501 ttgaagaata tcgagcgtcg ttatcatgat acgaacagtg atttcacgcg tgagcaaatg
    22561 cgtctttata tgacagaatt aacttgtcag acatgtaaag gttatcgatt gaatccccaa
    22621 gctctatctg ttcaaatcaa tggaacgaat atcgggcaaa caaatgagtt gtctattcaa
    22681 aaagcagtag atttcttctc aaacttaacg ttatctgaac aagagaaagt aattgccaaa
    22741 ccgattttaa aagaagtaaa tgacagactt tcattcttgg aaaatgtggg actagactat
    22801 ctaactttga gtcgcgcttc cggaacatta tctgggggag aagcgcagcg aatcagattg
    22861 gctacacaaa ttggatcgaa tctttctgga gttttgtata ttttagatga accatcgatc
    22921 ggattgcatc aacgcgacaa cgatcgactg attggctcat taaagaaaat gcgtgatctt
    22981 ggcaacaccc tcgttgtagt cgaacatgat gaagatacga tgtgggcagc agattatttg
    23041 attgacgtcg gacctggggc cggagaacaa ggcggacaaa tcgtggctgc aggcactcct
    23101 gaagaagtag ctaatgatga aaattctttg acagggaaat atctttcagg gaaaaaatca
    23161 atccctgtac caaaagaacg ccgtaaaggt aatgggaaag caatcaagat cactggtgct
    23221 tcagaaaaca atctgaaaaa tattagtgtt gaatttccat taggagaatt tgtagcagtc
    23281 acaggtgtgt ctggttctgg aaagagtaca ttggtcaata gtatcttaaa gaaatcgtta
    23341 gcgcaaaaat taaataagaa ttctgctaag ccaggtaaat tcaagacaat ttccggctac
    23401 gaaagtatcg aaaagatcat cgatatcgat caaagcccaa tcggccggac gccgagaagt
    23461 aatccagcga cttatacaag tgtatttgat gatatccgtg ggttatttgc tcaaacgaac
    23521 gaggcaaaaa tgcggggtta taagaaaggg cgttttagtt tcaacgtaaa aggcggtcgt
    23581 tgtgaagctt gtcgcgggga tggaattatt aagatcgaaa tgcacttttt gcctgatgtc
    23641 tatgttcctt gtgaagtatg tcatggtaaa cgatataatt ctgaaacatt agaagtgcat
    23701 tacaaaggaa aaagcattgc tgatattttg gaaatgacag tagaggatgc tgtagaattc
    23761 ttcaagcaca ttccaaagat tcatcgcaaa ctgcaaacga ttgttgatgt tggcttaggt
    23821 tatgtgacga tggggcaacc agcaacgaca ttgtccggtg gtgaggcaca acggatgaaa
    23881 cttgccagtg aattgcacaa aatctctaat ggaaagaatt tctatatact agatgaacca
    23941 acgacaggac ttcatagcga tgacatcgcc cgcttgttgc atgtattaca aagattagta
    24001 gatgctggta acacagtttt agtgattgaa cacaatctag atgtaatcaa aacagcagat
    24061 tatatcattg atttaggacc agaaggtgga gaaggtggag gaacgatcct tgcgactgga
    24121 acaccagaag aaatcattaa cgtaaaagaa agttatacag gtcactattt gaaaaaaata
    24181 atggtataaa gaggatagat agctgagata gttaaaaaaa cataaaaaaa tgaatttttg
    24241 cttgttttat taatatcagt gttctagaat cattacatgg tagttctttc tacactgatt
    24301 ggtatagata aaaacttcct ataagttcaa tccgttcaaa aaagaattga gcaatcggag
    24361 gactttcctt tgattgcttt tttctttatg gtatgatatt ccttgtgtag ttggaaaaga
    24421 aaaacaagag attatgagta agtgagagat aatgatgaac agtattgaaa atgaagaatt
    24481 tatccctata ttattaggaa gcgacatgaa cgtttatggt atggcacgtt cttttaatga
    24541 agcatacggc aaaatctgcc aagcatatgc ttcggatcag ttagccccta cccgctacag
    24601 taaaattgtc aatgtagaag ttattccagg atttgataaa gaccctgtat ttatcgagac
    24661 gatgcttcgt ttggcaaaag aaagatattc tgataagagt aaaaaatatc ttttgattgc
    24721 ttgtggtgat ggatatgcag aattgatctc ccaacataaa caagagctgt cagaatattt
    24781 tatctgtcca tatattgatt attctttatt tgaaaggctg atcaataaag taagctttta
    24841 tgaagtttgt gaagaatatg atcttccgta tcctaaaaca ttgatcgtca gagaagaaat
    24901 gttagtaaat ggacatttgg aacaggaatt gccttttgaa ttcccagttg ctttgaaacc
    24961 tgcaaacagt gtggagtatc tttctgtcca atttgaagga agaaaaaaag cttttatttt
    25021 agagactaga gaagagttcg atttaatttt aggccggata tacgaagccg gttataaaag
    25081 tgaaatgatt gtacaagatt ttatcccggg tgatgatagt aatatgcgtg tgctaaatgc
    25141 gtatgtagat gaagatcatc aagttcggat gatgtgcctt ggtcatccac tattagaaga
    25201 tcctacacca gcatcgattg gtaattacgt tgtgatcatg ccagattata acgaaaaaat
    25261 ctatcaaacc attaaggcat ttttggaaaa aatcgaatat acaggctttg ccaatttcga
    25321 tatgaagtat gatcctagag atggagaata taaattattt gaaatcaatt tgagacaagg
    25381 tcgtagcagt ttctttgtga cattgaacgg cttgaatctg gctcgttttg tcacagaaga
    25441 tcgagtgttc aacaaaccct ttgttgaaac gacctatgga acgaatcaat cagataaagc
    25501 aagattatgg atgggtgtgc ccaaaaaaat ctttttagaa tatgctcggg aaaatgaaga
    25561 caaaaaacta gctgagcaaa tgatcaaaga aaatcgttac ggtactactg tattttatga
    25621 aaaagaccga tcaatcaagc gctggttgtt gatgaaatat atgttccata actatattcc
    25681 aagattcaaa aagtattttc atgtgaaaga aggctagttc atggagaatt ttttcagtat
    25741 tttaggcgga atgggcacga tggcgacaga aagttttgtt cgcttgatca accaccgaac
    25801 gaaagctaca aaagatcaag aatatttgaa ttatgtctta ttcaatcatg caacagttcc
    25861 tgatcgtaca gcttatattt tagatcgatc agaagaaaat ccaatgccgt ttctgctgga
    25921 tgatattgag aaacaaaatt tattgcgacc aaacttcatt gtgttgactt gtaacaccgc
    25981 acactacttt tttgaagagt tacaagcggc aacggatatt cctatcttgc atatgccaag
    26041 agaagcagca aatgaacttg tccgtcagca tacgacaggg agagtagcga ttctaggaac
    26101 agaaggaagt atgaaagctg gaatctatga acgcgaagtc aaaaatcttg gtttcgaaac
    26161 agtgattcct gatactgcgc ttcaagaaaa aatcaattac ctgatctatc atgagatcaa
    26221 ggaatcggat tatttgaatc aagagctcta ttacgaaatc ttagaggaag cggtagagcg
    26281 cctaaactgt gaaaaagtca tcctaggttg cacagaatta tctttgatgc atgaatttgc
    26341 tgaagataat cattacccag tgatcgatgc tcaatctatt ttagcggatc gaacgatcga
    26401 acgtgcatta gctgaaagaa gcgaggcatt ggatacagca tcggaaaagt aaaaaaattg
    26461 gatttggggc aaatatccta aaagatattt ggtccaaatc tttttgtaaa agaaaaagaa
    26521 aactggtaat gatacgcccg ggatgatata attttattat gcacaatgtg agaggggttc
    26581 gtaaatatga cagataattt gcaattagtc atcataacag gtatgagtgg tgcagggaaa
    26641 acagtagcta tccaaagttt tgaagatatg ggctattttt gtatcgataa tatgccgcct
    26701 cgattgattc caaaattctg ggaattgatc aaagaatcag gtaaagtcac aaaaatggct
    26761 ttaggagtgg atttgcgttc tcgttccttc tttgaagaaa tccaaaacat gctgatcgaa
    26821 atcgaaaata cgaacttcat cgacacttct atcttatttt tagatgcttc agacgaagaa
    26881 ctagtggctc gctacaaaga aacaagacga gcacatccaa tggcgatgga cggtttgatc
    26941 actgaaggga tacgtaaaga acgggcgata ctagacgact tgaaagccca agcttccgtg
    27001 atcattgata cgacaacatt gacaccacga caattacgtg aaaaaatcaa tgagtcattc
    27061 aaatcaaagg gagaccaagg atttcggatc gaatttgtat catttggttt taaatacggg
    27121 ttgccgatcg attcggatat tgtgatggat gtgcgttttt tgcccaaccc tcattatatc
    27181 ccggaactaa gaccattgac cggaaaagat cagccagtct atgattatgt gatgtccttt
    27241 gatgaaacgc aatcattcta ccataaattt ttggatcttc tggaaactgt attgcctgga
    27301 tacattaaag aaggaaaaag cagcttgaca gttgcaatcg gctgtacagg cggtcaacac
    27361 cgttctgtgg cattgactga acgtttggca aatgctttag ggaaaaacta taaagtcaat
    27421 gtgacacatc gggacaagga taaaaggaaa gagacggtga accggtcgtg aaaatgaaaa
    27481 cctaccggat aagaaaacca aagatcgtag tggtcggcgg tggcactggt ctgccagtta
    27541 tcctgaaaag tttacgaaat caaggagcag atatcacagc agtagtgact gttgcagatg
    27601 atggtggaag cagcggtgca atcagagaat cgatcgctat ggctcctcct ggtgacttga
    27661 gaaatgtctt agtggcgtta tcggatatgc cacaattcta tgaagatatt ttccaatacc
    27721 gtttcaaaaa ggaagatcag tttttagcca atcatacgat cggtaatctg atcattgccg
    27781 cagtttccga gatgcgggga agcacttatg aggcgattca attattagca aaaatgatgc
    27841 atgtagatgg acatgtttat ccttcttcag aaactccttt gaccttgcat gctgttttta
    27901 aagacggaac gactgcagta ggagaatcta aaatcgcgat caaccgtaaa accattgacc
    27961 gtgtgtatgt ctgcaacaca aatgataaaa gtgaagccaa agcggctcga aaagtcgttt
    28021 cagcaattat ggacgccgac atggtcgttt tgggacccgg aagtttattc accagtatcc
    28081 ttccaaattt agtgatacca gaaatcggcg aagcaatgct caagacaact gctgaaaccg
    28141 tctatatctg taatatcatg acccaaaaag gggaaacgga acatttcaca gatgccgatc
    28201 atatacgtgt tttacacaag catttgaatc agccttttat cgatacagta ttggtcaata
    28261 cggaaaaagt gcctgaagat tatatggacc cagagatcta tgatgagtat ctagtacaag
    28321 tcgcacacga cttcaacgga ttgagaaatg aaggatgtcg agtgatttcc accgactttt
    28381 tagaattgaa agatggtggt gtcttccacg atggagacaa agtagtagaa gaattgttcc
    28441 gtttagtttt cggttcaaaa tattaagcaa tttattgttg gagaaaggag gagaatagga
    28501 tgtcatttgc tgcagatgtg aagaaagaac taactggttt ggaagttcat cgagaacatg
    28561 cgaaagcgga attagctgct ttgctgcgaa tgaacggctc tttaggtatt gtgaaccagc
    28621 agtttatttt gaacgtccaa acagaaaatg ctgcaattgc cagacgtatc tattctcttt
    28681 tgaaagatca ttatcaggta cgaagtgagc tactcgttcg taaaaaaatg aaattaaaaa
    28741 agaacaatgt ctatatcgtc cggttgaagc aaggcacaaa agatgtgttg atggatcttg
    28801 atatcatgga tggtatgatg ttcaatggac acgtttcgga cgagattatg ggaaattcgc
    28861 aaaaaatgcg ttcttactta cgtggtgctt ttatggcctc aggttcagtc aataatccgg
    28921 aaacgagtcg ttatcatttg gaaatatttt ctatttatga acagcataac caagacattt
    28981 gcgatatgtt aaattattac ggtctacatg ctcgaacgct tgaacggcgc aatggctata
    29041 tttcgtattt aaaaggagca gaaaaaattg ctgatttctt aacattgatt ggtgcaacca
    29101 actcaatgct ccgatttgaa gatgttcgaa tcgtacggga tatgagaaat tcggtcaatc
    29161 gtctagtcaa ttgtgagaca gcaaatctga acaagacgat tgatgcggct tctaaacaaa
    29221 tcgaaaacat cgaattgatc gaagcaaagg tcggtctaca tgcccttcct gaaaaacttc
    29281 aggaaatcgc agaacttcgg cttcaacatc cagaggtcag cttgaaagaa ttaggggaaa
    29341 tgattccctc aggtgcaata tcaaaatcgg gaattaatca ccgaattcgc aaaataaatg
    29401 aatttgcgga aaaactgcgt gaagaagcag tttaatccaa agaaaaaact gatacatgaa
    29461 gcaggagatg cgaatgtatc agtttttttt aaaagctata cttgcttttc ttccgtcaaa
    29521 ctataattcc aactttactg gagtaacata atgttgaaag gcttctattg ttttttaata
    29581 caatcgtata aaaagcgggg agacaactga atgaagattt tagttttata cataaacttt
    29641 tgcttaatat agaagtcctg atatagatag gatcaagtat ttatctataa ctggagatta
    29701 attaaaaact ggaaggaact gctcgatcaa ccttgagaac tgtttttctt acttctggtc
    29761 aagattctcg tggttggtgg ataaggatgg ttgataagtt cattggatta gctagtcgaa
    29821 aacaaccagg tgtaattaat gtagaagttt taacaagggg atggagtgat ttattcagtg
    29881 ataatcataa gttcataaat agtaattggg atgtatggtt ggtatacgtt atacctgaac
    29941 ttgaactatc aagatttggt ttagaatttg agaatcgatg tttataaagg gagggcaaaa
    30001 aatgtgcttt tttacaactt ttactatcat tatgattgca gtacttttta tttttctaga
    30061 tatagcaaag cgaaatacag catttttatt atatcgggtt ttacttcgag caggtctcat
    30121 aacatttata tcgatcgttg gattttttct attcacagtt attgtattta tttggagaac
    30181 tacagcacca ccattgccag agattacata tggagaattt ccgtttagtc ttgaatatga
    30241 aatagatgag gaaagaattt tgattgaaga tacggtgatt gcagaattta taggaagttt
    30301 tagagggaat gtgacaactc aacctagacg aagttggagt ataaaacttt taaataatag
    30361 tggagagttg aaagcatttc attatgaatt cttaataata gaaagtgaag atgtaacaat
    30421 cactttaagt agtggctctg ctggatattt tatgaatgat ggtggaagca agccacagct
    30481 cacagtccgt gatcgtattg caggttttat gcatttttct cactatgact tagaagaagc
    30541 tcgtaaaata cttgcagaat atgggatcac attaatcaat tgggaatatg cgccaccgat
    30601 aagaaatttt ttcaatgatt attattagtc ttataaaaaa cagaatgtaa aatttatgtg
    30661 cgcgagtatt tagttttcca ttttttgttt gatagatata tagcattgtt gaagcatggg
    30721 atagtggtgc caattttatc aatgaggttg gtcaggcagt taacataatt ggtgatgcta
    30781 taaatccatt taattggaat tggaggaatt aggtgtggaa acagtgagat tatggatatt
    30841 catgaaaaat tttcgttcgg ctttgagata tgttattctg gaatcaaaat caggttatta
    30901 tctgattgac acagatcact atactttttg gggaggctac ctttttccga tttttaattg
    30961 gataatcccc cataaggtta tgaaaattaa cttgcctgca gaagaaataa atagattaat
    31021 actcaacaaa gatttacaaa ggaaaagaga gaaagaacat tctaatgttg cttggatagg
    31081 tccagcattt gcagtagtga cttcttcaat tgtcggtcct atgctagaga gtatttatag
    31141 tgatttttct attggaatac gttatttatt tgttacttta agtatagctg ttcttttgtg
    31201 cataaagggt tatgttagca agaagaataa agatatttta acactaatcg gtgtaaacaa
    31261 tttatctagt actaaaatta tactgtttcc tgaatctata aaagaaggat caaggttgtt
    31321 gataattttt ataatgctta tactgttttc tgttttattt ggggtgatgt ttata
//
LOCUS       DACDXO010000029        31361 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E23605_2017
            SAMN12639989-rid9881463.denovo.029, whole genome shotgun sequence.
ACCESSION   DACDXO010000029 DACDXO010000000
VERSION     DACDXO010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639989
            Sequence Read Archive: SRR10023389
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741914.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:42:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,972
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 33 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 17 of 73
            Pseudo Genes (multiple problems)  :: 10 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31361
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639989-rid9881463.denovo.029"
                     /isolate="E23605_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(219..971)
                     /gene="xth"
                     /locus_tag="IWM35_001498"
     CDS             complement(219..971)
                     /gene="xth"
                     /locus_tag="IWM35_001498"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HAP9919358.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
     gene            1084..2004
                     /locus_tag="IWM35_001499"
     CDS             1084..2004
                     /locus_tag="IWM35_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP9919359.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQEADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            2077..3012
                     /gene="murB"
                     /locus_tag="IWM35_001500"
     CDS             2077..3012
                     /gene="murB"
                     /locus_tag="IWM35_001500"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HAP9919360.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            3310..4788
                     /locus_tag="IWM35_001501"
     CDS             3310..4788
                     /locus_tag="IWM35_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAP9919361.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVGICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITSRTNIYLSD
                     CLLEKFNKEQIIWKNPPTPDDWEYFGNTIIREKNRHLTEKIS"
     gene            5065..7125
                     /locus_tag="IWM35_001502"
     CDS             5065..7125
                     /locus_tag="IWM35_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9919362.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRREESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            7208..7777
                     /locus_tag="IWM35_001503"
     CDS             7208..7777
                     /locus_tag="IWM35_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9919363.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKPDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
     gene            complement(7952..8758)
                     /locus_tag="IWM35_001504"
     CDS             complement(7952..8758)
                     /locus_tag="IWM35_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9919364.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
     gene            8896..10410
                     /locus_tag="IWM35_001505"
     CDS             8896..10410
                     /locus_tag="IWM35_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HAP9919365.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
     gene            complement(10813..11796)
                     /locus_tag="IWM35_001506"
     CDS             complement(10813..11796)
                     /locus_tag="IWM35_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HAP9919366.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
     gene            complement(11801..12448)
                     /locus_tag="IWM35_001507"
     CDS             complement(11801..12448)
                     /locus_tag="IWM35_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP9919367.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            complement(12533..13165)
                     /locus_tag="IWM35_001508"
     CDS             complement(12533..13165)
                     /locus_tag="IWM35_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP9919368.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            complement(13181..13732)
                     /gene="coaC"
                     /locus_tag="IWM35_001509"
     CDS             complement(13181..13732)
                     /gene="coaC"
                     /locus_tag="IWM35_001509"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HAP9919369.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            complement(13729..14466)
                     /locus_tag="IWM35_001510"
     CDS             complement(13729..14466)
                     /locus_tag="IWM35_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HAP9919370.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            complement(14463..15452)
                     /locus_tag="IWM35_001511"
     CDS             complement(14463..15452)
                     /locus_tag="IWM35_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HAP9919371.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIIAFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            15710..16252
                     /locus_tag="IWM35_001512"
     CDS             15710..16252
                     /locus_tag="IWM35_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9919372.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            16269..17354
                     /locus_tag="IWM35_001513"
     CDS             16269..17354
                     /locus_tag="IWM35_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016182253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9919373.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            17366..18172
                     /locus_tag="IWM35_001514"
     CDS             17366..18172
                     /locus_tag="IWM35_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9919374.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            18173..19000
                     /locus_tag="IWM35_001515"
     CDS             18173..19000
                     /locus_tag="IWM35_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9919375.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            18997..20070
                     /locus_tag="IWM35_001516"
     CDS             18997..20070
                     /locus_tag="IWM35_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP9919376.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
     gene            20498..23194
                     /locus_tag="IWM35_001517"
     CDS             20498..23194
                     /locus_tag="IWM35_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP9919377.1"
                     /translation="MEYKYRIDILQPIVYYAFRHIKLEEKMKKSAKDRVFDELIRLTG
                     NMSSVTAEKISKNLGISRQNASHYLTRLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQ
                     INEAFHSVVGHDGSLREVIQKCIAAVKYPPNGLSVLINGATGVGKSFLATKIFEYAIH
                     EQIIEKDAPFAILNCADYADNPELLSATLFGYKKGAFTGAEKDTDGLLATANNGYLFL
                     DEVHRLSKENQEKLFLFIDTGNYRPVGENVNWHSAKVRFIFATTEKGENYLLDTFDRR
                     IQISVMLPTFEDRPIRERLELIQLFFQNEADVLQKDIIVSREALSLMLSHPFSGNIGK
                     LKNIIKISCADVYSRTQEEPLTIGKNEILIQLNMLESEVESLPIASLLIKKNQKIKTD
                     RIKPFDYDSILEKITQLINEGNFNENFSSIKNEVQHLVHHIDKDSFLSMSRKLMIQMF
                     RKVWVSVLGKTYGLTTSGPVAEIISRSYVISPSYKTDLTGINRTLAANLPRTTYLCNQ
                     FLEHLPPLSEKDENFLRLLIVVTLSDYVDESIELKGLLLAHGDSTASSIQAVVNQLCG
                     NYVFEALDMPVESNVSEIVESTRNFVKRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGE
                     LLLLNNLTTSIALDIGLKMTMNAPFKQIAEDAADKYSINVQYFEGFSQSSNIIISCMS
                     GLGISDKLKDVFRHHLSDESIEVFTKDYRELRELIDQNDETYFDKTKLVITTSDLPRS
                     FSIPNINVYDILDGDGAANLEHILSPEMKPAEFNRLIQELVKFFSLEGIADRLNFLNP
                     TIVMSEVETVISKYENYYHFTLNGRVKLNLYMHTALMMERLFLARTEKSEEPNYDLKP
                     DEDEFYYVSRSIFQSMEMKYNFKVNAYELSLLYELLGPYIQK"
     gene            23271..23759
                     /locus_tag="IWM35_001518"
     CDS             23271..23759
                     /locus_tag="IWM35_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP9919378.1"
                     /translation="MFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLSSIQL
                     LTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITKTFLE
                     KEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVNEEED
                     FF"
     gene            23771..24265
                     /locus_tag="IWM35_001519"
     CDS             23771..24265
                     /locus_tag="IWM35_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP9919379.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            24286..25095
                     /locus_tag="IWM35_001520"
     CDS             24286..25095
                     /locus_tag="IWM35_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP9919380.1"
                     /translation="MYDAFVVALAVFIGIAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            25092..25913
                     /locus_tag="IWM35_001521"
     CDS             25092..25913
                     /locus_tag="IWM35_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP9919381.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            25964..28063
                     /locus_tag="IWM35_001522"
     CDS             25964..28063
                     /locus_tag="IWM35_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HAP9919382.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            28060..29178
                     /locus_tag="IWM35_001523"
     CDS             28060..29178
                     /locus_tag="IWM35_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HAP9919383.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            29217..30095
                     /locus_tag="IWM35_001524"
     CDS             29217..30095
                     /locus_tag="IWM35_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP9919384.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            30286..30438
                     /locus_tag="IWM35_001525"
     CDS             30286..30438
                     /locus_tag="IWM35_001525"
                     /inference="COORDINATES: protein motif:HMM:NF016526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9919385.1"
                     /translation="MRIFTGGVHLNSNILCYFMSVVIVIVAYQCMFIFRNVDFIILGL
                     MFEFFW"
BASE COUNT        10113 a         5351 c         6506 g         9391 t
ORIGIN      
        1 tctgcggtaa taagcccaac caaacgaaaa atatgaggaa ctatttttgt ttggctgttc
       61 ttattacttg atgcaggaca gctttttcac aatctctcga taaacagtgt tcaaaagcca
      121 gtttctcggt attatgccaa atttatgcaa aatgtggaaa gccattttca caaaattttt
      181 cttaatactc gaaactaaac gactttttca caacctcttt atttcaaatc taattctact
      241 ggacaatgat cactgcccat aatgtccgta tggatctttg catcagctag tcctggctct
      301 aaatctttgc tcgtcaaaaa gtaatcgatc cgccaaccag cattgttttt ccttgaattg
      361 aatcggtagt tccaccaaga gtagacacct tcctgggttg gataaaaata acggaatgta
      421 tcgataaacc cgttatccaa cagtcgagaa agagccgctc tttcttctgg agtgaatcca
      481 gcattttttt gattcgtttt ccagtttttc aaatcaatct tttgatgagc gacatttaga
      541 tccccacaca cgatgactgg tttttgtttt tttagatttt ccaaataatt ataaaaggct
      601 tcttcccatt ctaatcggta atctaaacgt tttaattcgc tttgagaatt tggcgtgtaa
      661 caggttacaa gaaagaaatc ggaatattcc aaagtaatca gacggccttc tgtatcatgg
      721 atatcgatac ccattccgta agtggcattc aatgctggtt ttttagcaaa aatcgctgtg
      781 ccggaatagc cttttcgttc cgcgtaattc caatattgat aataacctgg aagatctaaa
      841 tcaatttgtc ctgcttggag tttcgtttcc tgcagacaga aaaaatcggc gtctaactca
      901 tgaaaaactt ctaaaaaatt tttatttacg atggcacgaa gcccgtttac gttccaagag
      961 ataaatttca ataaaagcac actccttcga attaatttcc ttacctttgc gatactattg
     1021 tacctcattt tagtaaaaaa tggtgtaaga tgaaagtaac taaatgatcc aggaggaaag
     1081 tagatgaaga ttggtgtaat cggtgtaggg aatatcgcag aaaaagcata tttaccaact
     1141 tatgcgaaaa aacaaggaac acttgatttt tattttgcaa caagaaataa agaaacaaaa
     1201 gaaagaattc aaaaagagta cgggttccaa catctatatg aaacgatcga tgagctttta
     1261 gaagaaaaaa tcgaagcatg tatgatccac gcagcaacaa aagtacatta tgaattagcc
     1321 aaaaaatgtc tagaaaatgg tatccatgtt tatattgaca aaccactgag tgtccatcta
     1381 aatgaagttc gtgaattgca agaattagct gataagaacc aaataatttt gatgatcggt
     1441 ttcaaccggc gctttgctcc tatggtagaa gaattgaaac gtatcccaga taagcggttg
     1501 atccacctgc aaaaaaatcg aatagcggca aaagaaacga cagagttcgt cctttatgat
     1561 ttgtttttac atcttattga tacagctgtg tatttattag atgaacccat tttgagaaca
     1621 aaataccaga taagagaaac aaaggaattt ttggaatatg ccattttaca attagaaaca
     1681 caaaatcaaa cggctatttt aacgatggat ttagcaagtg gcgctaatat agaaaaatat
     1741 caagtaacaa gtaaaaatgg tacttatgaa gtaaacgatc tgacccagct tgtcattcaa
     1801 gaggctgatg gaaaaaaagt gaaagaattt ggcgactgga caaacacgct agtcaaacgc
     1861 gggtttgagc caatggtaga ggccttcttt tctcagatcc gtttaggtaa atcggaaaca
     1921 gaattgctta aacaaaaaga agttgtaaga agtcatgaat tatgtgagga agtgcttaaa
     1981 gatcactacc gtcatcaact ttgaacaaga ctggcaagat tgtattaagc gtgatagaat
     2041 aatgaggacg tttgagaaaa gaaaggaaaa tcctcagtga ataaagaaga aatagttgaa
     2101 agcttagcca cgattactat attgtttgat gaaccattga tgaactatac gtttacgaaa
     2161 acaggtggac cagctgatgt ccttgcattc ccgaaaaaac aagaagaagt aaagcaaatc
     2221 atcgattatt gccgaatcca tgatatacct tggatggtat taggaaatgc aagcaatctg
     2281 atcgttcaag atggcgggat tcgcggtgta gtcatcatgc tgactgaaat gaaacagatt
     2341 catgtcaaag gaacgatggt cattgcggaa gcaggtgcct cactgattga tacgacttat
     2401 gcagctttgg cagaatcatt gactggattt gaatttgctt gcggcatccc gggaagtgtc
     2461 ggtggagcgg tttatatgaa tgcaggtgcg tatggcggag agatcaaaga tgtttttgct
     2521 gaagtggatc ttttgcttga agatggaaca ttgaaaactt tgacaaaaga cgaaatggta
     2581 ttttcttatc gtcacagtaa agtccaagaa ttacgtgcta tcgtattgga agctcgattc
     2641 agtttgcaaa caggggatta tgaagcaatc aaagcaagaa tggatgagtt gaccgaattg
     2701 cgtcaatcaa aacagccatt agaataccct tcatgcggaa gtgtctttaa gcgcccagta
     2761 ggacactata caggtcagct gatccaacaa gcaggattgc aaggcctgaa atggggcggt
     2821 gcacaagtct ctgaaaaaca tgctggattc atcgtcaata tcgatcatgc gacagcgaca
     2881 gattatattg aattgattgc gcacatccaa gaagtaatca aagaaaaatt tgatgtgtct
     2941 ttagaaacag aagtgcggat cattggagaa aaacaagcaa tatcagaaac aagcagaagt
     3001 ttagccaatt aataagtaat ggaacttaaa ataagtaaaa gagcaggacg gtggccgtcc
     3061 tgctcttttt tgtggttgtt tacgcaaatg gcgcaaatct tgtacatttt aacacaataa
     3121 aacaaaaaat atagaatatt tcctcataaa tttttccatt ttttgcaata ttctcaaaaa
     3181 aaagataaaa aatgagaata actgatgaaa aaacaagcat aatccatcat aaaatcagta
     3241 taggtgaaaa gatgaacttt catctgcata tcaagtaatt ggtatgaggc attttttgag
     3301 gtgtaggata tgaagttgga ttaccttata gaagaggaag atagagaaaa actgctgcta
     3361 gctagatatt tagaatttga gaaaaagaat tatgtagtgc tgcctaagtt atatgaaaca
     3421 ttaggtttgt ctaaatttaa aacaagaaat tatatcatag aattaaataa agaatttact
     3481 gaattcaaaa caaagccaga gatccaaatt tcagaagaag cagagatcca tgtcgaacgt
     3541 ctgaatttga cggatatcaa aaatttaaga agaatctact ttcaacgatc aaaaattgct
     3601 cgactgttaa cggagatcgt ggattcaaaa gtaacgatcg aaaaatttgc agagaatcat
     3661 tttttaagca tatcaagagc ctatgtaaaa agaagagaat tagtctcttt tcttgaacag
     3721 catcaaataa agttaaagaa aaacatatta gttggggaag aaataaacat acggaacgta
     3781 ttatttgggg tatatttcgg ggtctttaat ggtttttcta tgccatttag agaaagcgtc
     3841 atgaatgaag caaagagtat cgtgcaatat tttgaatatc tgttcaattt aaaactgtct
     3901 gcaacaaaac gaatcaaatt caatttgcta accactatta gtttgttgag ggcctatggg
     3961 aatcaaccaa tcggaaaacg tttcttttca gatgaatact ttaagagctc agaaggaatg
     4021 aagatcatca aggatttatc acatatttct tttcttcata tccctaaaga gcagatcatt
     4081 gatgagattt cttatatcat gttgtttttg atcacggaag aagaatttga attagatggc
     4141 gcatttggac ttgcggaagc tcattttcat gatatccaca tgattagtca aaaaatgtca
     4201 gtggaattga ttgatcaatt accattttcg attgaaatct ctgaagaaga aagactatat
     4261 atacgaaaag aacttcgtat gggtatgatg catgtcaatc gaaaaaattg gatattcgac
     4321 tttgagatga ctagctttac gtctaaaaag cagctacagt ttttcttcga gacgtatcca
     4381 atattcagta cagcaatcca aaaggtcata caagagaata aacgctattt cttgggaaaa
     4441 gatgaacgtt tcatgattcg cttattttat gattatctct ttttattggt cggtatttgt
     4501 ccggtatcat attttgaagc accgattcat gtctgtattg atttttcgca aggatctgct
     4561 tataccgatt acattatttc tcaagtgaaa ggatttaaaa attacaatat tgtgattgag
     4621 agtcgaatca cttctcgtac gaatatttat ttatctgatt gtttacttga aaaatttaat
     4681 aaagaacaga taatttggaa aaatccaccg actccagatg actgggaata ttttggaaat
     4741 accatcatca gagaaaaaaa tagacattta actgaaaaaa tcagctagta tttatggatg
     4801 gcagctttgt ctcacatatt tattagctga tttatgtttt tttcaaagaa aaggtaacta
     4861 gatataacta gatataacta gatatattac ttaacaaaat taaaaagtac ataagttatt
     4921 attccgattt tcttgaaaca ataaaaaatc aaaaaaaata ctctcctaat agtccatctt
     4981 cttttgattt tatttatata atgaaatcat cttatgaaaa ttactacttc atgtttagac
     5041 aagagtaggg aggactaaga ataaatgtgt tcaaaaagac gttcaaaaca actaaataaa
     5101 cacgcttgtc gtttagataa ggaacggtcg tgttctgaaa aacactacgt taagtttatg
     5161 atgcgcacga tgctgatcag tttatctgta ggtgtctata tgcatgtacc cgttcaggca
     5221 acgacacaaa ccatcaaggt cgtacgtacc tctgtttctg agaccactga ttcccctatc
     5281 actattactc atactttcac tgatgcaaat ggaaaacagc tacttcctgc attgacttcc
     5341 tataataaag tcagttatcc tccaacgata aaccactact ggttaattcc taacaaaaca
     5401 ttatggacat cggaaaccgg aagcagtgtg ccgctttcta tcttttccga tatgactgat
     5461 cctacaaaag gtgatcagtt aaaacagatc actgatattt tgaatcatgc tttgacagat
     5521 ataaaggacg gtggaagcgc acaagtttct tatgtatatg aaaaagatta ttcacagttt
     5581 gaaagaaaga aaatcgatgt tctggcaggg acaaaagttt cgatatctga tctggtagaa
     5641 aggctgaaga acgcagatgg aacacaagga gatatgtcca aagtaatttc aaacaatggg
     5701 aatatcgtgg atacgactaa tacaggaaca tctactattt ccttgtccta ttttgatcca
     5761 attgctttaa aaacaatgac tgcatctgct gtgatccatg ttcgtgctga tcaaacagct
     5821 atcaaaggaa aagatcttca cttaagaaca ggagagagtt acgcccctaa agatcttgtt
     5881 gaatatgtaa caaaaagcga tggaacttca ggaaataaag aagaaatcaa aatgaacggg
     5941 acagtagata ctacaactcc gggagaatat cccgttgtat taacttatat agactcgaaa
     6001 acatcaaatg tggcagaaac aacagctgta gtttatgtag aagcatctgc tgcttcatcg
     6061 actgtcatca ctcattttat agatagtcaa acaaataaag aacttcaagc agaaacacat
     6121 atgagtagtg taaatcgtat tcctgatttg cgcctacctg ctgaattttc tgatcgagtg
     6181 ccaaatatag atttatcgac ttatgaacaa aatgggaaag tcacttctat ttctgattgg
     6241 atgcaagcaa acggattaga cagaaaaagt cactggtcgg agatccttga agtacttcac
     6301 tcagaactga tgaataatag gaagcaatca ggaggggaaa ttacctttac ttatgtttat
     6361 ataccagaca atcgttcatt agaaggtcat gatcttattg ttcatccaaa cgaaaaaata
     6421 aacaaagaag agttgatcca atcagcaaca aattccttag gtaaacccgt accgttaaag
     6481 gatgtatcgg ttacaatcaa tgaagtacca ttggaggatg gttatttgac caaacaaaca
     6541 ggtgatttta cgattatgta tagttatcat gacccgtatt cactccaaga actccatgct
     6601 cagtcactat tacatgtttt accattaaac aaacagatag atcaaaaaga acaaaaaaga
     6661 cgagaagaaa gcttgtcaga aaaaaaacaa caaatggctc atgcagtttc tccaacaggg
     6721 aaagtaaaac aaacaagaag cctagagtta gcaagaatat ctagaaaaat ctcagaatct
     6781 aataaaacga taaggcaaga agctcatttg gccccacaag tttctggatt ggaaaatgaa
     6841 attttagagc ctaaaataaa acaacaatca gaaaaagaat ttgttgctaa acagtcggaa
     6901 gatcagaagg aatcagatga ccagaaggaa acgaagaaaa aacgcttgtc aactattgat
     6961 tccaccgatg agcatctgca gccgcaagta ccacttccta gtccgggaac acccgcacct
     7021 ggtggaggta ttccttcttc tgctttagga gatttcatgc gaggtatggc aggaacatgg
     7081 gtatacgcta atcgaaatga atttgacgca gatgactggt tctgatttat atctctatct
     7141 gttatagtca aataagtagc tgagtcatat gttatttggc ataatggaga cgaggaggga
     7201 tagaaaaatg ttagataaaa cgttacctta cgcagaaatc tggatgacaa gaccacttca
     7261 acaaaatgtt ccaatcacgc ctttagcgga aggttttgaa tttcgatttt atcaaaaaga
     7321 agatgaatct gcttgggcgg agattgagac ttccgttggg gaatttcaaa cgacagatga
     7381 agcacttgag tattttcaac aaaactttgc tccttatcct gaagaacttg aaagaaggat
     7441 gttttttgta gagacaaaaa aaggagaaaa agtagcaacc tgtaccgctt ggcgaaaaaa
     7501 atcaagagag caagtgtatc cggtttttca ttggttagca gtgaaaccag attaccaagg
     7561 aaaagggctc gcaaaagcat tgactgccag agtcttgcaa gaatttccta ccctccatac
     7621 ggacggacca atctatcttc acacacagac atggagccat caggcgattg ctctttataa
     7681 aaaaatgggt tttactttta ttgctgaaaa tctagacggc acacctaatc cagattatga
     7741 aaaagttatg caggttttaa gtaaattaga taaataacga aatggtgaaa taagtcctca
     7801 gtattgggat aaaaaacgaa atagtcattc ctcatatctt acttttttga gttaatacta
     7861 tcggataact aatggcatgc catacattca aaaaagaagg ggcagtgaca aaagtattgt
     7921 cactgcccct tctcttgttt tttagcatgt tttatcgttt gattttttta ggaataaaat
     7981 tcttgatgaa cgtaaatttt tggtaatcgt ttcgactata gaggtaacca aatacactga
     8041 aaacaactgc tccaataaaa ttaacaaata aatccttcat agtatccatg attcccacgt
     8101 ctaagtaacc attttggatg attgttttat ctgttttacc atctgcattt tgactttcaa
     8161 tgattgtccg agtgatatct ggaacatgat agacagtgtt tccgtcttta cttaactcca
     8221 cactattgat ttgctggatg atcgtatctt tttgcatatc gaattgcagt aatttatctg
     8281 ctgaaaattc cacaaactcc cacataacac caactgtcat cgaaaaacag aaggaaacaa
     8341 ctgctagtaa taaaggagtc aagttcatgg tttttgtatt tttattgagc aaataaacaa
     8401 gggaaaagcc gacaccccca gctaaaaatc cgctgaatgc atgtagaaaa gtatcccaca
     8461 ttgaaaaata gccataaaaa tcacttaact ctcctaaaac tgttgaacta aaaataaaag
     8521 ataacaaaat gatttctagc atatttggaa tctgtatctt taatatcttt tccagaaaat
     8581 aagggatttc aaataaaatt aatgtcagta ataaaagcat tgtatttccc cagtgttggt
     8641 tccagacctg atccactagc ataataacga cgatgatacg taataaaata tagattccgt
     8701 ttgtgattct acctgcagtt tttgtactgt tcgtaaaaaa ggttttcaaa tgattcataa
     8761 aataactcct tcatgtatat ccttaattat actaagaagc gtgtgtttac caaagctata
     8821 tgacttaaag aaaaacagag ttgatattgt gaaaaaaatc acttaatata gtataaggag
     8881 aaaaggggga tacaaatgaa aaaattagtt gctggtttat taacagccgt tgcacttctt
     8941 gctatgagcg catgcggcgc agatcagaaa aatacagcgc agacaagttc tagtacgaag
     9001 gaatctgccg aagtatcatc aggcgcttct gaacaagtat atacagatcc aaaagaaatg
     9061 aaagaatctt atgatgtcgt gattattgga gctggtggtg cagggatggc agcagctatc
     9121 gaagcaaaag atgctgggtt gaatccagta attttggaaa agatgcctgt tgccggagga
     9181 aacacatcta aatcatcaag cggtatgaat gcttcagaaa caaaatttca aaaagaagaa
     9241 ggaatcaaag actcgaatga tgccttctat gaagaaacgc taaaaggtgg tggaggaacg
     9301 aatgataaag aaatgcttcg atactttgtc gatcactctg ctgatgcggt cgattggcta
     9361 gatcaaaacg gtatcaaact ggacaactta acgatcactg gagggatgag tgaaaagagg
     9421 acacatcgtc cgtctgatgg ctcggctatt ggtggatact tagtggaggg gttattgaat
     9481 aacgtccatg agcgggaaat ccctattttt gtcaacgcag atgtgacaga cattaagaaa
     9541 aatggggaaa cggtcaatga agtgactgta catgttcaag gagaagaacc aaaagaggtc
     9601 accggaaaag caatcgtagt taccacaggc ggttttggtg ccagcaaaga atttattgaa
     9661 aaatatcgtc ccgatttagc tgattatatt acgacaaatc aagaaggatc aactggagat
     9721 ggtatcacca tgattgaaaa attaggtgga caaaccgttg atatggataa aatacaaatt
     9781 catccaactg tgcagcagga tgagggggtc ttgattggtg aggttgttcg cggagaagga
     9841 gcaatcttgg tcgatcaaga ggggaaaaga tttgtcaatg aaatggatac aagggataaa
     9901 gtatctgcag caattacagc attgcctgaa aaatcagctt atttgatttt tgaccaaggc
     9961 gtgcgtgatc gtgccacagc tattgaattt tatgaccaaa aaggatatgt cacaactgga
    10021 aagacaatgg aagaattagc agaaaaaatc gatttaccaa aagaaacact agcaaaaaca
    10081 gttgatggat ggaatacgga tgttgcggag aaaaaagatg ctcaattcaa ccgtgccaca
    10141 gccatggaac atgacttatc gtctccgaat tattatgcga tcaaaatcgc accaggaatc
    10201 cattacacga tgggcggtgt gaaaatcaat acgaacactg aagtattaga taaagaaaac
    10261 cagccaatta gggggttata tgctgctggt gaagtcacag gtggcttaca tggaaacaac
    10321 cggatcggtg gaaattcagt tgcggagatc attatttttg ggcgacaagc gggcatacaa
    10381 gcagctcgat ttgcttcgga aaatgaatga aatagaacgt aaataaaaag aggttgtgaa
    10441 atcagcgttt tgagctaata ccgagggtca gctgattgaa cactgtttag tgaagaggtt
    10501 gtgaaaaagc tgccctgcat caagtaataa gaacagccaa gtaaaaacag cttcttatgt
    10561 ttttaacttg gttggactta ttaccgcaga tgcaagcttt tgaacaccgt ttattcggaa
    10621 aaagagcgtg ggacaaaagt aaagaacact tttgtctcac gctctaaaca cgtataaacg
    10681 gcggaagcag aagaaattcc ttcggaaata agctgaaatt cacaaaaatt tgaaaagcaa
    10741 ttttcgtgaa tttcttctta tttcttggag ttaaacgctt ctgtcccagc ctcttttttg
    10801 atcagcatga ttttattcag ttatcttcaa aatgattttt ccgatgtttt tgtttgcttc
    10861 catatattca tgcgcttgtt tgactgcgct gaattggaat gtacggtcaa tgattggccg
    10921 aatcttgcca ttttctagaa aaggcccagc tttttgcata aaagtttgtg cgagctctgc
    10981 tttatactga tcacttctag gtgtcaataa tgtaccaagc agttggacac gtttcccaat
    11041 caatacacca aggtctatct caggcacgat ggtcccacct agcataccaa tcaagaccca
    11101 acgtccatca acggctatag aagctaaatt cttttcccag taagaagcgc cgatgaaatc
    11161 caaaataaca tcgactcccc gaccttcagt gatttttcgt acctcttctt gaaagtcttg
    11221 ttttttatag ttgatcaatt catctgcacc aagttcttta caaaaggcga gtttttcttc
    11281 tgatcctgca gtaacgatga ttttcgcgtg agaaagctgt ctagctaatt ggatcgctgc
    11341 tgttcccaca ccacttgctc ctgcatggat caagacagtt tcgccttctt gtaattttcc
    11401 aagccagtaa agtgtttgat aagcagtcaa aaaaacttct gagattccag cagcagaaac
    11461 atagtctagc gtatcaggga gaaggattgc acgatcggca ggcatcacag catattcggc
    11521 ataaccacct agattgacta atccgtatac ttttgtccca ggagtaaaag tggagatatc
    11581 acttttattc tcaactacct ctccagctac ttcgactccc aaaatagggt agggaggttt
    11641 caaagagact gattctcttt tcataatatc tgttcgattg acagctgctg tatggacttt
    11701 gatcagtaat tcgccttgtt ttggtttagg aacaggcact tcaatttgtt ctaatacatc
    11761 tacagaacca ggatgcttga ttgaaatggc acgcatggat ctattcctcc tttgcccatt
    11821 cttggaatgc tgtattcaac tctttgctac cttcagaaag tactagagtc gtacgagaaa
    11881 tatccgtttc ttccactaga gcggcaagta cagcagcaac tgtagagcga gtgatcgttt
    11941 ttgatgcctt ctcattaggc ttagttaatt gaatggaagt aacttcttca tcgttagtca
    12001 atgtgactgg ttgaagaatg acaaaatcta agttagttcg tttcagccat tcatctgcat
    12061 aatgtttagt aatataataa tctgtcatgg actcaggcca ttttgctggt tcatctgcat
    12121 aaacagcact gaccatcaga tagcgagaga catgcgcatt ttcagctgcg atcactgttt
    12181 tgattgcgcc atccaagtct acttgcagaa gattctttcc ttgagagccg gcagcaaaaa
    12241 tcaatacatc gatgcctttg aatgcttcgg tcattttttc cacggaccaa gttaaatcaa
    12301 aagagacata cgaaacattc tcttttttta ctaatgtttg ggaagcaaca tctctcaccc
    12361 ctgcaaaaat ttcatgggaa cttggagcta aatcttttat caggtgtcgg ccaatttgtc
    12421 cgtttgcacc tacaacaaaa attttcattt ggatcatcct ttcgacgtca aatctttcca
    12481 ttcaatagta acaccaaaag agaaaaaaat cagagatcgg ctacgatgac tatcagttag
    12541 taagagaagg gtgcttgaag tatcttaaca agacattgca tactgctgca gtcgcaagac
    12601 ctgcgacgat tccttcaggt ataccacttg taaaaataac cgctagaata gctgaataaa
    12661 cagcatttaa atttgttccg atgactttgg cataatcttg ctggaataaa aagtagatca
    12721 tattcatcac taaaatcgta ttggtcaatg aaccggaaag tccagctaag aataaagaaa
    12781 cggaatctgc tttttgtttc attagtttgc gcagaccttt ataaacgaaa taaggaacca
    12841 ccccaatcaa gatgcgagga ataaacgcga tcagcaaggc tttccagctt ccatgttctg
    12901 ttccgatgac tgggataaac ggagaaaaga caaaagacaa aggtgtttgg ataactgtgc
    12961 tacggatcat gctgatcagt ccaaatgtac cgccaagaaa agcacctagt cttggtccta
    13021 aaacgatcga agcaatgatt acaggaatat gcatggttgt tgcattgatt ggaccgatag
    13081 ggataaaacc taaaggcgta acagctaata aaatcaaaat agctaaaaac atcgcagtta
    13141 atgtaaagtt ttttgtattt ttcataagta ctcctttttt ctaagcattt ctttcattaa
    13201 gagtcagcat gacctgttcg atgatcgtct gaatatcagc taaggcacct tttccaaaat
    13261 ctccacaagc taaaagagat tctcttggtt caatttcctg atagccgaca gatctcaatg
    13321 tttccagatt acgctgagtg atcgggtttt gatacatatt cgtgttcatg gcaggggcaa
    13381 tcaattttgg tgttcccact ggaagcgcca ttgcgactgt tgaaagaaga tcgtctgcaa
    13441 tgccgtttgc tagcttgccg ataatgtttg ctgtagcagg cgcgatgaga aacaagtcag
    13501 cttgttttgc tagttcgata tgattgatta cctgcggatt tgattcttgc atgacgtctg
    13561 tatggactgc gcgcttcgac agagattgca aggttaatgg cgtgataaaa tgtgtgctgc
    13621 ttttagacat aatcacatct acctgggcac ctaatttgac gaattgactt gtaagatcag
    13681 cgcttttgta agcagaaata cttccagtaa cgcctagtaa gatctttttc attttgattc
    13741 ctccagtcta attttctcag taatcaatgc agcaatctca gatttacttt gagcttcttc
    13801 cactgttcca tctttagata agagatagcc gtggtgtgtc tcgccatgga tttccgtcaa
    13861 atcatttgcg aaaataaaat cagttttgtt atttatcaga ctattttttg cgactgccag
    13921 aagagattcc tttggtacat ctactaatag tttgaaacca atgatttttg cttttggctg
    13981 ccattcacgt agataagaaa tgatttttgg tgttttcttc aaaacgagca caagatgctc
    14041 ggtgttagaa gagatctttg aaacagtggt atctgtctgt tcattttccg caaaccaatt
    14101 gtccagttct tcttgcgttg aagaagtggg taggtttttt gccagctgtt cttctgaaaa
    14161 actaaaagca ggagtaaaat cactgactgc catactgtgg ataatcgcat cgtagtgttc
    14221 ttttttcgtt agttcttcta attgtaaaaa taagtccagt gcagattcga tcgtataaat
    14281 cgttatattg gagtgtcttt tcggtttcag tgcttgtttc gtagtcacgt aatcgattgt
    14341 ggtatccgga ttggcagcta aataatcagc taatgcttgc cctaatcgtc ctgtcgaatg
    14401 atttgtaata gcacgtactt gatcgatttt ttcagtcgtt cctccagctg tgattagaat
    14461 cttcatttat ggtcacctac atttactagt gttcttcctt ggtgtgttcc agcaagtagt
    14521 ttagatgctg tttcaggcaa ttgatccaga gtaatctctt ggacgaccag ttcatctgca
    14581 atctgcaaat ccgccatctg ttgccataaa gaaagacgtt gatctattgg tacattgaca
    14641 gaatcaattc caatcaactg gatgtttcgc agaataaaag gcaagacagt cgcgtttagt
    14701 ttgattcccc cagcattgcc acataaagct actgcaccgt tataagagat caatggaaga
    14761 agactgctta gctgctcgcc acctactgtg tcgatgacat agtcgatttg ctgttttcct
    14821 aaagcttttg ttgtctctgg cagaaattca tcaggtcgta cgacttgcaa agcacctaga
    14881 ctttttagcc atgtgacaga ctcttctttt ctggaaaagg caatgattga ctggaatcct
    14941 agtttatgta acagggctat cgctgtactg cctacaccgc cagaagcacc agtaacgaca
    15001 acttttttat cggaggtcat accttgacgg attaaagcgt tcacacacaa catagcagta
    15061 aaccctgctg taccaaggat catcgcttga cgcagcgtca tattttttgg tagaggaact
    15121 aaccagtctc ctggtacttt ttgatattgg ctataaccgc ctggatgggt gacgccaagc
    15181 ccaaaaccag tagcaagtac aagatcttct tttgaaaaac gtggattgtt actttggacg
    15241 acagttccgg ataaatcaat ccctggagtc atcggatatg ttcgtatcac ctgaccagac
    15301 tcgctcattg ccaatgcatc tttataattg acatcagaat aggcaatttt taccaagact
    15361 tcattttcag ccaactcagt aaatggcaga tcactgattg ctgtatggaa aacaggttcc
    15421 tttattacct gaaaagatcg aaaagcgttc attcgtgaga cactcctttt aaaattttct
    15481 tttatcatac cataaagcct actcttttat tcttgttctt ccgtaaattt agacaaaaat
    15541 taggtgaaaa ataaagaatc aaacaactag aatttattcc aaaagataaa gaaatattca
    15601 gcaaaaaaaa ttttacaaag ggttgccttt ttcaataaat ttcgtatact aatacgagtg
    15661 gtagtaaaca ggaagtttac tattagaaaa cttcgattcg gaggaaatta tggaaattgg
    15721 tgagaagtta agaaatttgc gcatccaaaa gaatttgaca caagaagaac taggcgaacg
    15781 cacagatttg tcaaaagggt atatttccca gttggaacga gatttaagtt caccatcaat
    15841 ggaaacattc ttttctattc ttgaagtctt aggtgtgact ccagaagaat tttttcatca
    15901 agaagcagcg aatctgcaag tggtttattc aaaagaagat catacggttt atttcgatga
    15961 agaaaacggg tatgaattgg aatggctcgt ggctgattcg aatgaaaaag aaatggagcc
    16021 tgtcttgctg acttttgaag gtgcaggaga atataagacg tttgaacctt cattatctga
    16081 aacgtttatt tttgtattag aaggggaatt agagttaagc ttaggagaga cggtctatgt
    16141 ggcaaaagca ggacagtcga tctattatca agcgacaaaa caacatcaat taaggaatca
    16201 tctcaaaaaa cgaacaaaag tattgattgt tgcaacgcaa tcatatttgt aatatagggg
    16261 gtaaaaacgt ggggaaacca atcatttcat ttgatcaagt agtgaaacgc tatgatgatg
    16321 aaacagtatt aaaaaaagta agttttgaga tcgaacaagg aaaattttat acactgcttg
    16381 gtccttccgg ctgtggtaaa acaacgatcc tacgtattat agctggattc accgaagcta
    16441 cagaagggga catctatttt gaagggaaac ggttgaatga tttgccagct aataagcgac
    16501 aagtaaatac agtcttccaa gattatgcac tttttcctca tatgaatgtt tttgataacg
    16561 ttgcttttgg attgaagatc aagaaaatgt ccaaatcaga tatcgaaaag aaggtaaaag
    16621 atgctttgcg tatggtccag cttcctggat acgaaaaacg ggagattagt gaaatgtctg
    16681 gtggtcaaag acagcgtgta gcgattgcac gagcaattgt aaatgaaccg aaagtattgc
    16741 tattagatga accgttatcg gcgcttgatt tgaaactacg tacagatatg cagtatgaac
    16801 tgcgggatct gcaacaacga ttaggaatca cgtttatttt tgttactcat gaccaagaag
    16861 aagcgttggc aatgagtgat gagatttttg tcatgaataa agggaaaatc gtccaaagtg
    16921 gtacacccgt agatatttat gatgagccaa tcaatcattt cgttgctgat tttgttgggg
    16981 agagcaatat cgtggacggt gtaatgatcg aagataatct agttgagttt gttggtaagc
    17041 aatttgaatg tgtcgatggc gggatgcgtc taaatgaacc agttgaagtt gttttacgcc
    17101 cagaggattt gacgatcacg acaccagata aaggaaaact agtcgtaacg gtcgatactc
    17161 agctgtttcg tggtgtccat tatgaaatca tctgttatga tgaacagcaa aatgaatgga
    17221 tggtccattc aacgaaaaaa gctaaagaag gaagcaaagt aggattggcc tttgagccag
    17281 aagatatcca tgtcatgcgt ttcaacgagt ctgaagaaga attcgatgca cgtcttgaca
    17341 gctacgaaga atagggggga caagaatgaa aacaatgcgc aggatctatt ccgtccctta
    17401 tattttgtgg ctcggtttat tcgtgatcgc gcctgtatta atgatcattt atcaatcttt
    17461 ttttgatatg aatgggcaat tcacgctgaa taattatcta tcttatttta cgtcggggac
    17521 atcgctttct atgacgatca attctgtatg gtatgcattt ttgatcacgt ttttcacgct
    17581 gatcatcagt tacccgactg cttatttttt aacaaaactg aagcacaagc aattatggct
    17641 gatgctgatt attttgccta catgggtgaa tctgctgctt aaagcttacg catttattgg
    17701 gattttcagt gtgcacggca gcgtgaatca tttctttgat tttatcggga tcgggccgaa
    17761 acagatatta ttcacagact tcagttttct gttcgtggca acgtatatcg agattccatt
    17821 tatgattatg ccaattttca atgcattgga agagatgaat ccttctttga tcagtgctag
    17881 tagagattta ggcgcgaata gctttgagac cttccgacga gtgatttttc cgttatcatt
    17941 aaatggtgtg aaaagtggtg tccaagcagt atttattcct tctctttctt tattcatgct
    18001 gactcgtctg attggcggaa atcgggtgat cactttagga acggctatcg aacagcattt
    18061 cttggttaca caaaactggg ggatgggttc tacaatcggt gtgattttaa tcattgctat
    18121 gttcatcgtg atgctcctga caggcgagaa gaaaaaagga ggtcgctcat aaatgaaaaa
    18181 atttaaatgg tcgtatcttt atttagggat cgtcttttta ttcttatatg caccgatctt
    18241 ctatctgatt ttttattcct tcaatgaagg aggaacgatg aacagcttta caggatttac
    18301 ttgggaaaat tatgaagctg tttttgaaga tacacgtttg atcattatcg tattgaatac
    18361 gttcatgctg gctttcttat ctgcattgat cgccacgatc atcgggacat ttggggctat
    18421 gggaatctat tacacgaaaa aacgacgtgc acggacagcg ttgttgagca tgaataacat
    18481 cttattagtc tctcctgatg ttattatcgg tgctagtttc ttgattttct ttacactact
    18541 aggattcgga ttaggttttc agagtgtatt gctttcccat attgctttca gcattccaat
    18601 cgtcgtcttg atggtcttgc caaaattgca ggaaatgaac gattcaatgg tcgatgcggc
    18661 tagagacctt ggtgctaata atttccaagt agtcaaaaat atcattttgc cttatctttc
    18721 tccaggaatc atagctggat attttatggc atttacctat tctcttgatg attttgcggt
    18781 tacatttttc gtcacaggaa atggctttac gacgctctca gtagagatct attctcgtgc
    18841 aagacaagga atcagtttag aaatcaatgc gctaagtgcg ttagttttct tgttctccat
    18901 gatattggtc attggatact actttattag taaagaaaac cgtccaaaac ggctgaagaa
    18961 aaacagaaga gtacgcgagg gggtggcacg gctgcgatga aaaaattaca atctctgttt
    19021 ttaggtattc tcatcattat tgcggtgctg ctgcttggtg ttcgtcaact tgaaaaaaca
    19081 agtggcatga gtggagcaaa ggtacttacc atctataact ggggagatta tatcgatcct
    19141 agtttgatca cgaaatttga aaaagaatat ggatacaaag tgaattatga aacatttgat
    19201 tctaatgaag cgatgttcac aaaaatccag caaggtggga caaactacga catcgctata
    19261 ccaagtgaat atatgatcca aaaaatgatc aaagaaaaac tagtacttcc attagatcat
    19321 tcgaagatca aaggattaaa taatattgat ccacgatttt tagacttgga ttttgatcca
    19381 gataatcagt attcgattcc atatttttgg ggaactttag ggatcgtata taacgataaa
    19441 ttctttgatg caaatcaaat taagcattgg gatgatttgt ggaagccgga attaaaagac
    19501 agtttgatgc tgattgacgg tgcacgagaa gttatggggc tttctttgaa tagtttaggc
    19561 tattctttga atagtaagaa tatggcacag ttgacagaag cagcaaataa attgaatcat
    19621 ttaacaccaa atgtcaaagc aatcgtggca gatgagatca aaatgtatat gatcaatgaa
    19681 gaagcctccg tggcagtgac attttctggc gaagctgccg atatgatgtg ggaaaatgaa
    19741 catttacatt atgtgatccc accagaagga tccaatcttt ggtttgataa tatcgttatg
    19801 ccaaaaaccg tgaaaaataa agaaggtgcc tatgatttca tcaactttat gctagaaccg
    19861 gaaaatgcgg cacaaaatgc agagtatatt ggttattcta caccaaatga agaagcaaaa
    19921 gcgttattgc caaaagacat ttcgtcggac gaacaattct atccatctga tgacacgatc
    19981 tcccatcttg aagtctacaa agatctagga tcgaaatacc taggcatcta taatgaccta
    20041 ttcttagaat tcaagatgta taggagataa tggcaaaact cagtaagaat ggggacttat
    20101 caacgttcta aagagctaaa aatcgtcctt tttctatctg agttttccaa tcttctacca
    20161 aaagcggatg aaacgccgct ttttttgtgt tattttattt tttctaccac gtttttctac
    20221 cagagaaaag tggacaaaac cattgtaaaa tagggcccga gacgggctag taaatctaag
    20281 attgaaaaag ttacagaagg ataaagtaat tggaaactat gcaaatatac ttaacccttc
    20341 tgtaatctat taataaaact aatatatgaa tccagttcaa tatttgtagc tatgaaaaaa
    20401 cataaaaatc atattagata aaaactaaat tggtagataa attattttgt cagatatctg
    20461 aggcaaatat tcgactattt gtgtttcaaa gtaaaaattg gaatacaaat atagaataga
    20521 catattgcag ccgatagttt attatgcttt tagacacatt aaactggagg aaaaaatgaa
    20581 aaagagcgct aaagatcgag tttttgatga gctgatacga ctaacaggga atatgtcttc
    20641 tgtcactgcc gagaaaattt cgaagaatct aggtatctct cgtcagaatg ccagccatta
    20701 tttgacgaga ttagtggaag ataaaaaggt cgagaaatta ccgggaaagc ctgttttgtg
    20761 gaagccttta gatgaatatg cggttgtaga taatacggag caaataaacg aagcctttca
    20821 ttctgtagtt ggtcatgacg gaagtctgag agaggtcatt caaaaatgta tagccgctgt
    20881 caaatatccg cctaacggac tcagtgtatt aataaatgga gccacgggtg ttggaaaaag
    20941 ttttttagcc acaaaaattt ttgaatacgc tatccatgag caaattattg aaaaagatgc
    21001 accatttgct atcttaaact gtgccgatta tgcggacaat cccgaactgc tttcggcaac
    21061 actgtttgga tacaagaagg gggcctttac gggggctgaa aaggataccg atggtttatt
    21121 ggcaacggcc aacaacggct atctttttct cgatgaggtc catcgcctat cgaaagaaaa
    21181 tcaagaaaag ctctttctat ttattgatac tggaaactat cgtccagtcg gtgaaaatgt
    21241 aaattggcat tctgctaagg ttcgttttat tttcgccacg acggaaaaag gtgaaaatta
    21301 tttattagac acctttgatc ggcgtattca aattagtgtt atgttgccaa cttttgaaga
    21361 tcgccccatt cgagaacgat tagagctgat tcaattattt tttcaaaatg aagcggatgt
    21421 tttacaaaaa gatattattg tttcaagaga agccttgtct ttaatgttat ctcatccctt
    21481 tagtgggaat attggtaagc tgaagaatat cattaaaatc agttgtgcgg atgtgtatag
    21541 tcgaacacaa gaagaaccat taacgattgg gaaaaatgag atcttgattc agttaaatat
    21601 gctggaatcc gaagtagaaa gcttgccgat tgctagttta ttgataaaga aaaatcaaaa
    21661 gataaaaacg gatcgaatca aaccgtttga ttatgattcg attttggaaa aaattactca
    21721 gctgataaat gaaggtaatt tcaatgaaaa tttttcttcg ataaagaatg aagtacagca
    21781 tttggtgcat cacattgata aggattcttt tttatcaatg agtagaaaat tgatgattca
    21841 gatgtttaga aaggtctggg tatccgtttt aggaaaaacg tacggattga cgacgagtgg
    21901 acctgttgca gaaattatta gcagatccta tgtcatttcc ccaagctata agacagactt
    21961 aaccgggata aatcgtacat tagctgcaaa tctacctcgg acgacttact tatgtaatca
    22021 gttcttggag catttaccac cgctctcaga aaaggacgag aactttctaa gactgcttat
    22081 tgtcgtgact ttatctgatt atgtagatga atccatcgag ctgaagggcc tgttgcttgc
    22141 tcacggagat agtacggcta gcagcattca agcagtagtg aatcaattat gcggcaatta
    22201 tgtttttgaa gcgttggata tgccagtaga gagtaatgtg tcagagattg tcgaatcgac
    22261 aaggaatttc gtgaaacggc aagtgaatac agatagtcta gtgctgatcg tagatatggg
    22321 ctctttaaat caaatttaca cacagatcaa gaacgattta aaaggggagt tacttcttct
    22381 gaataatttg acgacttcca ttgcacttga tattggcttg aagatgacga tgaatgcgcc
    22441 atttaaacag attgcggagg atgcagcaga caagtattca atcaatgttc aatactttga
    22501 aggcttttct caaagtagca acattatcat ttcctgtatg tcgggtttgg ggatttcgga
    22561 caaattgaaa gatgtttttc gtcatcattt atcggatgaa tccatcgaag ttttcacgaa
    22621 ggactacaga gaactacgtg aattaatcga ccaaaatgat gaaacttatt ttgacaagac
    22681 aaaattggtc atcacgactt cggatcttcc acgttccttt tcaattccta atattaatgt
    22741 ttatgacatc ttagacggtg atggagcagc caatttggag catattttga gtcctgaaat
    22801 gaagccggca gaatttaacc gcttgattca ggagctagta aaattctttt cgttggaagg
    22861 aattgcggac cgtttgaact ttctgaaccc aacgatcgtg atgagtgaag tggaaacagt
    22921 catttctaaa tatgaaaatt actatcattt cactttaaat ggccgtgtaa aactaaatct
    22981 ctatatgcat acggcattga tgatggaacg tttattcttg gctcggacag aaaaatctga
    23041 agaaccgaat tatgatttga aaccagatga agatgaattt tattatgttt ctagaagtat
    23101 ttttcaatca atggagatga aatataattt taaagtcaat gcttatgagc tttcgctgct
    23161 ctatgaatta ttaggaccgt atattcaaaa ataaagtgtc taaaaaagtg tctaatctgt
    23221 tttttttagg ttagacactt tttttaatgt gaattaacgt gattaaacca atgtttttaa
    23281 aagttggcac aggaattgct tatataaaag tgaacagaaa gaaaggaatt aaccatatga
    23341 agaaaattct aatagcgact cacggtcact tagcttcagg ctttcttagc agtattcaat
    23401 tactgacggg gaaggttcaa gagattacgg tgattaatgc ttatgtggat gagtgtgatt
    23461 ttgaacagga gttgaatgta tttgtaaacc aggtgaacga gcaggatcag atttttgttt
    23521 ttaccgattt atttggcgga agtgtcaatc agaagattac caaaaccttt ttggagaaag
    23581 agattgctgt aactattttg tctggcttta atctgccaat tctgctcgaa atagtattgg
    23641 caaccaagga attaacaaag gaggatattc aggaactggt aaataaatgc caacaggaat
    23701 taaagatgac cattcttgat gaggtacatg tagtaaatga agaggaagat tttttttagg
    23761 aggaagaaaa atgattaaag cagtgcgaat tgatgaacga ttaattcatg gacaagtagc
    23821 gatgacttgg acaaagagtc taaatttaac cgggttagta gtagcaagcg atgaggcggc
    23881 taccaacgat attcaaaaga tgacattgaa gatggctgca ccatcgaatg tcaaagtcat
    23941 tattaaaaca gttgatgacg cgattcattt gttaaaagat ccgcgggcga aggacatgcg
    24001 cttgatggtt ttagtcccta atgtcaaaga tgcggtaaaa gttgcgaaag ctttcccaac
    24061 ggaaatcgaa ttaatgaacg tggggaatgc tgggaaaatg acaccgccat cggaagataa
    24121 gaaaacctta tcaaaggaag tgatgttaac gacgtcagaa attcaagact taaaagaatt
    24181 agttgaactt tatccagata ccgtttttca gcctacaccg gcgatggaaa aacgttcagc
    24241 tatctctatt ttgagtgatt tttagaaaaa ggaaggagaa aaattatgta tgatgcattt
    24301 gtagtcgctt tggccgtttt tattggaatt gctggacatg aaatgttcgg gatgtccatg
    24361 ctgagtcgtc cgattgttgt tgctccgtta gcaggagcgt tgatggggga tttgcagacg
    24421 gggttgattg tcggggcgtc actagaagcc atctttatgg gtgtcgtcaa tatcggaatc
    24481 tctagtacgg cggagccttc tttggctgct gggctggcta cagcgttcgc aatcaaaatg
    24541 ggaggaaacg tggatgcgat tattcccatt gtctttccac tagcagtatt aggattacag
    24601 ttaatgaata tgattttttc ttttgtatgt ggaccgatgg ctgctaaatt tttcaaattt
    24661 gcaaaatctg gtgatcaaaa aggaatcatc aatctgcact tctttatgtg gtttgtccac
    24721 tatgcgcttt atgctcttat tccattcttt gccgttttat ttggagcaga tgttgtcaaa
    24781 agcgtcttgg ataatattcc agaggtcatc atgaacggat taacagtcgc tggaaatctg
    24841 ctgccggcag ttgggatggc gatgctgtta cggatgcttt ggagtaaaaa tattgctgtg
    24901 tggtttttct ttggggccgt tgttatggcg tatctcgagt taccattgat tgcaattgcc
    24961 gcagttggaa cgattattgc aattgttata gcccaaagag atctacaact aaaaaatata
    25021 tctgttcatg cagttcaaag cgatgctgct gtaaatgcaa cgcttgacca agaagaggag
    25081 gatttctttc aatgaaatta gctgatggaa tttctaaaga tgagaaaaaa ttggttcgga
    25141 agatgttttg gcgctcgatg actatgtatg cttcggtgaa tccggtgacg atgggcggcg
    25201 gtggtttcgc ctattctatg atgcccttca ttaatcattt ctacaaaagt gaagaagatc
    25261 gagctgcggc gctggaacgt catacacagt atttttcgac gaccattcct tgcgcgtcct
    25321 ttgtcatggg gattgcggca tcgatggaga aggaaaattc tgaaaaagct ggttttgacg
    25381 tggaaagtat caacgcgatc aaaacgagtt tgatgggacc attggctgga attggcgatt
    25441 cattgttctg gggagtttgg cgtgtcgtta ccgcgggact agcgatcaac ttagccaatg
    25501 ccggaaatat tttggcacca attatctttt tagttatgtt caatatacct aattacttga
    25561 tgcgatacta tggcggcgtt ttaggctatt cattaggctc taaatatatt gaaaagctgt
    25621 atgcgaacgg gttaattgaa attctgacaa aagctgctag tatcttgggt ctgatcatgg
    25681 taggtgcaat gacgatcagt atggtcaaat tcaataccgt tttgagcttt tcaatgaagg
    25741 gcgaagaact aatgaatgtc caagaaatcc tcgatagtat ttttaaagga attgtaccgt
    25801 tattggtaac attgagctgt ttcaaattac tagataaaaa agtcggcgtc atcaaaatta
    25861 tttttggaat ggtcgtcttg ggaattttat tgtcattact tggaatcgca tagtaggaag
    25921 ctgcagctac taggctgcag tttttttagc aaggagggaa aagatgacta ttaatgaagt
    25981 aatttattca cccaaacacc cattttttaa tgctgagtta aacgacggaa atgtgactac
    26041 ttcaaacgaa tacagctact atcggaatat tcctgatgta aagggattga ccttcgatgt
    26101 aagtaatcaa aagacattag caaaccttga tgtttgcggg acgattcaaa cgttgacctt
    26161 ttatcgagaa aatttactaa ctgaggaaaa accaggtgtt tgggtcaata aacagctctc
    26221 tcaaaccaat tcgttatcct taaaggttga agtaaacggg attgtctacc atttgtcaga
    26281 atcggatcat cgtattgaag tcgatgtgat tcaagactgt ttgcctagat atattcatca
    26341 ttattcgacg tttaaggtca ttgtagtatc ctttgctccc atactggcta ataagcgttt
    26401 aagtatgctt gttcaacagg tttacattgt gaatgaaaca cacgaaagcc ttcaagtttc
    26461 agtgaaagat gtgccttcgt atcaagagaa gtattcggat cagcaaaatg tgttgatttc
    26521 tcaaaaagga aataaggaca cgatcgcaca acaggaagtg gcaagcttcg ccattgcttt
    26581 tgtggaccca aatgcttttg aagaagtaat gacttttcag gaatctgata tcgaaaaatg
    26641 gctgaaggaa acattgaact attttgctca gctatttggg cagctgtcaa tgccaaacga
    26701 tagagttgtc catctctatc gtcgtgcact ttatcaaagc ttttctagtt tcggtatgaa
    26761 tcgacaggga caggtcgtcg gcagcaattg gggcagctat cctgctacta atcgaatttg
    26821 gaataaggat atgtattacg catcattgcc attcaccatg tttgaaccgg agttatgtca
    26881 aaaaacggtt ctatggtttg atcaatatgg cgttaagttt cctggttcaa aatttacagg
    26941 aggtatcaat cattctctga gtaattcgtt atccagtgcg ttactggcta gtctttattt
    27001 tgaacatact ggggatacaa aattctttga gcggtgtccg caggttctac tgaatgcttc
    27061 aagaattatt gaggacatat tagctcaacg tcggtcaggg gaaccaatgc tttttccatc
    27121 tgtttggtta tcagacgcct ttgcacttgg aaagtatcat accggcagca atctttgtct
    27181 ttggaaggcc tgtattggac ttgcagaaat ttttgaagcc ctagaacaac tgagtcttag
    27241 caagcgatac aaaaacattg cctgtaaact aaaggaatca atttgtaaac aaatgactat
    27301 cgatggtcct tttggtgagc aatttttgga gggaatcggt gatgaggaga aaacaaccta
    27361 ttcagtgaag cactatcaaa aaccgattct tgagcaaggg ttaatctttt taagtgatgt
    27421 gattgtggat ggaaaaatta atttattgat gcatgatgga gaagaatcag acactacgtt
    27481 gatgcctttt tatcaatttt tagataataa agatcattta tatcagaaca ccatggcctt
    27541 ttctgctagt tctttcaatc caacctatag cgaggaaatc aaaggcatca cttggggatt
    27601 agaatccggc gccacttttc ctggctttat cactgtttta atgagcgagc tgcacaaccc
    27661 tcaagctttc gtaacccgat tagaagaact aatttgtttg gcagatttag acggttcgtg
    27721 gtggtggtgg ccgtatcgct tagaggctca acaaggagaa gtcgtgcgag actttggctg
    27781 cggcaaatgc ggctgggcat cgggtctttt tgtcagccta atgatcagtc aatatttcgg
    27841 attgaaattc aaaaaaggag tattgcaaat tgatcctgtt gataatttaa cgttttcttg
    27901 gaagaatttg aagttcggtc aagccttcat ttcgattgaa tgtacgcaat ccgagttgag
    27961 tatttcaaat ttgtcagaaa agccattgaa aataagtgat acgaaaaaaa ttcttcacgt
    28021 agaaaaggga aaaacgattc acattcaaag gagaaaaaga tgagtatact aaaaaatatt
    28081 acccaattta aaaaaatcca gagtcgagca atcactcccg aaaatccaac aggcgagaaa
    28141 ggtaaagctg cgatgttgcc aagcgagcta ggaccaagcc gcaaaggtcg tggcgcaatt
    28201 ccgctgactt ctggtgaaga aactgtgatt gcggatatta ctggaacggg aataattcgt
    28261 catatgtgga tgacgattcg tgatcgaaca gaatttggca gttttgtttt acgtgattta
    28321 atcttaagaa tttattggga tgggtcggat agccctgctg ttgaaagtcc tttaggggat
    28381 ttcttttgta atgggtttgg agagcgttgc gaggtgaact cgttgccgat agtggttaat
    28441 cctacagggg gctttaattc ctatttcgaa atgcctttcc aaacatccgc taaaattacg
    28501 atcgagaatt tacatccgaa ggatatgtcc tcattttttt atacgataaa ttacgctttg
    28561 gtggacgaat tggaagaaaa tagcctatat tttcacgcaa attggcatcg ggaacgcatt
    28621 accaaattac aggaggatta taagttaatt gatcgattaa agggtcaagg ctattacgtg
    28681 ggtacttacc ttgcgcttac ctgcctagaa cgttattggt ggggcgaagg cgagtttaaa
    28741 ttctacattg atggggatag tgagtatccg accgtaacct caacaggttc agaagattat
    28801 tttggcgggg cgtgggcctt tcatgagtac gatgagttcg gacgtccaca tgcgaaaaac
    28861 tattcaacag cctttttagg gtatccttac caatcaaata gagatgccac aagagatcgt
    28921 tttgaaacgg gaaaattgaa tgccgttcat gcctttggtg acgacgcaat gccacgacat
    28981 ggtctttatc gttggcatct tcaagacccc atctgtttta ccgaagattt atcggtcact
    29041 ttacaacaaa tcggcaacga tgatgttcgc ttatttgagc ggcaagacga tgttgctagt
    29101 gtcgcttact ggtattcaac agaagcttca ggaaatacta aacgtcattt cactgatcga
    29161 gaacgcttgc cgcgttaatg aagtgttttt cacatattcg aagggaggaa cctatgatga
    29221 tatatggggc aattgaagct ggagggacta aatttgtctg tgcagtcggt actgaggaat
    29281 taacgatttt ggaaagagtc agttttccca caacaactcc agttgaaaca atgaagcagg
    29341 tcattgattt ttttaaacct tttaaggaac aattggcagg catcggggtc ggctgctttg
    29401 gtccaattga tattcaatgc aaatcaacga gtttcggtca tatcacatca acaccaaaac
    29461 ttgcttggca aaatttcaat tttgtaggaa cgctaaagca atatttcaat attccgattg
    29521 aatggacaac agatgtcaat gcggcttgct atggtgaata tgttgctggt tctggcaaag
    29581 ggttagcagg tgttgcatac ttcactgtgg gaacaggtat cggaggtggt gccttgttca
    29641 atgggacttt cgtggaaggg ttcagtcatc cggaaatggg gcatatgttg atcaagcgac
    29701 atccaaagga caatttttca ggaaactgtc ccttccacca agattgttta gaggggatgg
    29761 cagcaggacc agcgattgaa aaacggttgg gtgtaaaagg ccagaattta ttggctgacg
    29821 attcgttttg gcagattgaa gccttttacc ttgcccaatg tgcatacaat acaacactga
    29881 tgttttcgcc agatagaatt atttttggcg gtggtgtaat gaaacaagaa cacatgaaga
    29941 aaaaagttca ggataaattt gttgagctta taaatggata tgttgaaata ccaccaattg
    30001 atagctacat tattacgccc gaattaggtg ataatgccgg aataatcggc ggattagcat
    30061 tggccagaaa agcggtaaga aataagcaac cataattaat gaatgtttca ttgatactca
    30121 tgtcaaaagt atttaaccga gtaatcaaat gatcaaatga agttggataa agcgaaaatg
    30181 aattcaaata aaaaggctct aagtcaaatc gtgttgtttt cattaaatca ctgaaaggaa
    30241 atgcttctgc gtttcctttt ttctttttgt tgtgcgctgt ataccttgag gatattcact
    30301 ggtggcgtac acttaaatag taatatattg tgttacttta tgtctgtggt aatagttata
    30361 gttgcttacc aatgtatgtt catttttcga aatgttgact tcataatcct tggcttaatg
    30421 tttgaatttt tttggtgata gattttttca gccgtataaa ataaatcgta attctattat
    30481 agagagttgt gattataaaa agatagcaag aaaaaaaaag aaagtttcta tacattttaa
    30541 atattttatt cctatttcta ttgctaaaaa agtagaaatt ctatatttac ctttaactct
    30601 cgcttctctt gcaaatgtag ttagcatttt aatcgataat aaactcaggt agttatatta
    30661 ccttgaaatg attgataatt ttgtcacttc tatttttttt gcaataaatt ttttatttta
    30721 gtgaaaaata agaaactatt tctttgaagg cgataagtta gaaaaatatt attgtagtct
    30781 tttctttatt aggaatcatc actgttgtat cttattacct tttctgtttt tcttattaga
    30841 gaaaaataat ttgagaggta gatagttttt attggaggaa gtaattatgt atatttttga
    30901 aacaaaaatt tttcattgca ataattgcaa caaagaaatt tcatcggggg aagaggtttg
    30961 gacaaaaatg gaaatttccg ccaaaagtaa ctgctcctca actgaaatca attaaagaac
    31021 tagaatttga gaatgcactg gtaatttgta tgacatgtac tgatgaagta tttcgaaaac
    31081 atttttaaag gttgagatgt atatagattt aaccaatcat ttatctctct cggaaaagga
    31141 atattaacta accagcgggt tatcaaaaat aggtaggaaa actgtactta atggcttatt
    31201 tcgcgctgat tatgagaata tcagtctgtt tgattaattt ttagggttca aaatacagag
    31261 acaaactcca ggtcgggttt cgccacaagc gaattacccg atctggagtt ttgtctattg
    31321 caggaaggcg tagccggact gcaggaataa agcccccggt t
//
LOCUS       DACDXO010000030        30288 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E23605_2017
            SAMN12639989-rid9881463.denovo.030, whole genome shotgun sequence.
ACCESSION   DACDXO010000030 DACDXO010000000
VERSION     DACDXO010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639989
            Sequence Read Archive: SRR10023389
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741914.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:42:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,972
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 33 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 17 of 73
            Pseudo Genes (multiple problems)  :: 10 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30288
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639989-rid9881463.denovo.030"
                     /isolate="E23605_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            185..1123
                     /locus_tag="IWM35_001526"
     CDS             185..1123
                     /locus_tag="IWM35_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HAP9919386.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            1146..1826
                     /locus_tag="IWM35_001527"
     CDS             1146..1826
                     /locus_tag="IWM35_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAP9919387.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            1799..2623
                     /locus_tag="IWM35_001528"
     CDS             1799..2623
                     /locus_tag="IWM35_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAP9919388.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            2790..3608
                     /gene="purR"
                     /locus_tag="IWM35_001529"
     CDS             2790..3608
                     /gene="purR"
                     /locus_tag="IWM35_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HAP9919389.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            4111..5484
                     /gene="glmU"
                     /locus_tag="IWM35_001530"
     CDS             4111..5484
                     /gene="glmU"
                     /locus_tag="IWM35_001530"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HAP9919390.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            5578..6549
                     /locus_tag="IWM35_001531"
     CDS             5578..6549
                     /locus_tag="IWM35_001531"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HAP9919391.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     regulatory      6555..6657
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            6723..7292
                     /gene="thiT"
                     /locus_tag="IWM35_001532"
     CDS             6723..7292
                     /gene="thiT"
                     /locus_tag="IWM35_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HAP9919392.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNPWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     gene            complement(7373..8146)
                     /locus_tag="IWM35_001533"
     CDS             complement(7373..8146)
                     /locus_tag="IWM35_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9919393.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIW
                     KLKGTRYREEEIIATLIYSVTDRHWQLVEDADLSYLPLK"
     gene            8413..10113
                     /locus_tag="IWM35_001534"
     CDS             8413..10113
                     /locus_tag="IWM35_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HAP9919394.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGGGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            10083..10301
                     /locus_tag="IWM35_001535"
     CDS             10083..10301
                     /locus_tag="IWM35_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9919395.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            10321..11211
                     /locus_tag="IWM35_001536"
     CDS             10321..11211
                     /locus_tag="IWM35_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HAP9919396.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFVLDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            11229..11513
                     /locus_tag="IWM35_001537"
     CDS             11229..11513
                     /locus_tag="IWM35_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9919397.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
     gene            12000..12614
                     /gene="gmk"
                     /locus_tag="IWM35_001538"
     CDS             12000..12614
                     /gene="gmk"
                     /locus_tag="IWM35_001538"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HAP9919398.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYVNKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEKRMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
     gene            12619..12924
                     /locus_tag="IWM35_001539"
     CDS             12619..12924
                     /locus_tag="IWM35_001539"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HAP9919399.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            13177..15585
                     /gene="priA"
                     /locus_tag="IWM35_001540"
     CDS             13177..15585
                     /gene="priA"
                     /locus_tag="IWM35_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HAP9919400.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            15600..16091
                     /gene="def"
                     /locus_tag="IWM35_001541"
     CDS             15600..16091
                     /gene="def"
                     /locus_tag="IWM35_001541"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HAP9919401.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            16084..17022
                     /locus_tag="IWM35_001542"
     CDS             16084..17022
                     /locus_tag="IWM35_001542"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HAP9919402.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKKAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            17022..18380
                     /gene="rsmB"
                     /locus_tag="IWM35_001543"
     CDS             17022..18380
                     /gene="rsmB"
                     /locus_tag="IWM35_001543"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HAP9919403.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKMLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            18393..19133
                     /locus_tag="IWM35_001544"
     CDS             18393..19133
                     /locus_tag="IWM35_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HAP9919404.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            19130..21199
                     /gene="pknB"
                     /locus_tag="IWM35_001545"
     CDS             19130..21199
                     /gene="pknB"
                     /locus_tag="IWM35_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HAP9919405.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            21364..22263
                     /gene="rsgA"
                     /locus_tag="IWM35_001546"
     CDS             21364..22263
                     /gene="rsgA"
                     /locus_tag="IWM35_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HAP9919406.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            22276..22926
                     /locus_tag="IWM35_001547"
     CDS             22276..22926
                     /locus_tag="IWM35_001547"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HAP9919407.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGRFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            22927..23565
                     /locus_tag="IWM35_001548"
     CDS             22927..23565
                     /locus_tag="IWM35_001548"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HAP9919408.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            23720..24574
                     /gene="mreC"
                     /locus_tag="IWM35_001549"
     CDS             23720..24574
                     /gene="mreC"
                     /locus_tag="IWM35_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HAP9919409.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            24578..25087
                     /gene="mreD"
                     /locus_tag="IWM35_001550"
     CDS             24578..25087
                     /gene="mreD"
                     /locus_tag="IWM35_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HAP9919410.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
     gene            25342..26916
                     /locus_tag="IWM35_001551"
     CDS             25342..26916
                     /locus_tag="IWM35_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9919411.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTTAPESSATEE
                     STTVPESSATEESTTVPESSTTEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTP
                     EQPKPVTPAPAPSGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTG
                     RDIGGWTVPQESAGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESV
                     KVGSVQWFAPDFAVSM"
     gene            complement(26984..28783)
                     /locus_tag="IWM35_001552"
     CDS             complement(26984..28783)
                     /locus_tag="IWM35_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HAP9919412.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
     gene            28906..29412
                     /locus_tag="IWM35_001553"
     CDS             28906..29412
                     /locus_tag="IWM35_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HAP9919413.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSINHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            29491..30213
                     /locus_tag="IWM35_001554"
     CDS             29491..30213
                     /locus_tag="IWM35_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAP9919414.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
BASE COUNT        10000 a         5457 c         6491 g         8340 t
ORIGIN      
        1 tttttattgt agtgaaatgg ctagatatga gcagcaactc atccatttta cgatttaatc
       61 aagaatatac atgcttaaat cagaaattga tggatttcta ttgactgtgt ttttttattt
      121 tgatacatta gttcacgtta ataaatcgta aaattccgat ttgttcattg aaaggagcat
      181 ataaatgaaa aaatatcttt tgacagctac ggtaatgata ggtgctttac tgtttgcagc
      241 atgtggaaat acgaacaagg aagcagacaa aaaagaggat ttgacgatcg ttacgacttt
      301 ttatccgatg tatgatttta caaaagagat cgtaggggat gaaggaaacg tgaaactttt
      361 gatccctgct ggaacggagc cgcatgattt cgaaccaagt gcaaaagaaa gagcagaaat
      421 ttccgatgca gatgttttcg tgtataatag ttcagacatg gaattttttg tggattcgtt
      481 aaaggattca gtggatagca aacagacctt gatgatcgaa gcagctaaag gaatcgaccg
      541 tttagaatcg caagaagcag acgaacatga agaatcagaa gaagggcatg gtcattcaca
      601 tgaatacgat ccacatgttt ggctcgatcc ggtattagct ataaaagaag tcagaacgat
      661 tgctgaagaa ttaggcgaaa aatatccaga taaaaaggaa acttttacga agaatgccga
      721 tgcttatatc aaaaagcttg aagcactgga tcaaaaatac tccgatgagc taaaagatgc
      781 aacaaaccgc acatttgtca ctcagcatgc tgcttttgct tatttagcga atcaatacga
      841 cttaaaacaa gtagcgattt cgggcgtttc accagatcag gaacccacac caagtcgctt
      901 agctgagcta aaagaatttg tgaagaaaaa caacatcaaa gtgatttatt ttgaagaaaa
      961 tgcctcttcg aaggtagctg agacattgtc gaatgaaaca ggagtgaagc tagaagtgtt
     1021 gaatccttta gaaagcttga ctaatgaaca aatcaaagca ggcgaaaatt atatttctgt
     1081 catggaaaaa aatctgaaag ctttaaaaga aagtatcaat taagttttag aatggaggtt
     1141 gtaaggtgcg ttatatcgaa atatctgatt tgacctttta ctatgatgat gaacctgtac
     1201 tagaagatgt ctcttatcat gtagatgcgg gtgaatttgt cattttaact ggagaaaatg
     1261 gagcagccaa gtcaaccttg gtgaaagcat cgttaggatt actgaaacct tcaaaaggaa
     1321 tgataaagat tgctgagaaa aatcaagaag gaaaaaaatt aagtatcggt tatattcctc
     1381 aacaagttgc atctttcaat gcaggttttc ctagtacggt gatcgaatta gtccgttcag
     1441 gaagatatcc aagaggccgc tggttcaagc gtctctctga aaaagatcat caacatgtac
     1501 agcgtgcttt agaagctgta gggatgtggg atatgcgaaa tcggcgaata ggtgaattgt
     1561 ctggcggaca aaagcagcga atcagtttag cgcgtatttt tgctactgac cctgacttat
     1621 ttgttcttga tgaaccaaca acaggtatgg atgaagaatc cagaaatgaa ttttatcgtc
     1681 ttctccgaca tagtgcgcat gtccatggga aatcagttct aatgatcacc catgatcatg
     1741 aagatatcaa acagtatgcg gacagacaga tccgactggt gagaaaggag gattctcaat
     1801 ggcgttgctt tcatatgaat tcatgagaag agcgtttctt gccgctatct tcattgccgg
     1861 aatcgccccg atgctaggtg tgtttttagt gatcagaaga caatcgctga tggctgatac
     1921 gctttctcat gtctctttag caggggtagc gttaggattc tttttgaacc ttaatcctac
     1981 acttacgacc atgctagtcg ttgtattagc agctgtgata ttggagtatc tgcgaacgat
     2041 ctaccgaacc tattcagaaa tctccattgc cattttaatg gctggtggtt tggcgctagc
     2101 gttagtattg atgaatttga gcggagggaa ttcagcaaca agtatccaat cctatctgtt
     2161 tggttcgatc gtgaccatta catgggagca agtcatcttt ttaggaatct tattcgttgc
     2221 catcggtata ttgttctttt tattcaaacg gccgatgtat gttctgactt ttgatgaaga
     2281 taccgcccac gtggatgggc tacccgttca tttgatgtcg atgatgttca atgtgattac
     2341 tggtgtggct attgctgtga tgatcccgat tgccggtgca cttttgatat ccgcaatcat
     2401 ggttcttccc gcagcaatcg gtatgcgatt aggcaaaagc ttcaatgtcg tgattgctat
     2461 cagtgtttgt gtaggatttc tagggatgct gtcaggacta actagctcgt tttatctaga
     2521 tacacctcca ggagcaacaa tcacattagt ctttattgct ttgttcctga ttgtaaatat
     2581 catcaaacga atattggttg ccttcagaag aaaaaataac taaattataa agaaaagaag
     2641 ccttataaaa acgaaaaatt ttttataggc ttctttttat tttaataaat accgaactat
     2701 tttcttattt tttcataaaa tgctcgaatt tttgaagaaa aacttatata atgtaaaacg
     2761 ataaaatttc ttaaaaggag ttggaatagg tgaaagtacg tcgcagtgaa cgtctagttg
     2821 acatgacaca gtatttattg aatcacccgc atgaattgat ttccttaaca agctttgccg
     2881 aacgttatga atctgctaaa tcttctatca gtgaagattt gggaatcatc aagaaaacat
     2941 ttaaagaacg agggtatgga accttggaaa caatcccggg tgctgcaggt ggtgtattgt
     3001 tcatccctga aatttcatat gaagaggcga aaaaatatat tgaatctcta gcagaacgtc
     3061 tatcagaaca agaccgtctg cttccaggag gatatgttta tctctctgat ttgttaggag
     3121 aacctgaact gctgcgtcaa gttggaagaa tcattgcttc taaatacctt ggtgaaaaaa
     3181 tcgatgctgt aatgactgta gccacaaaag gcgtacctat tgcccaagct gtgtcctatt
     3241 atctgaatgc gccattcgtt attgtacgcc gggactcaaa aataacagaa ggatcaacag
     3301 taagtgtcaa ctatgtgtct ggatcttctg aaagaattga aaagatggaa ctgtctaaga
     3361 gaagtttgaa acgtggttca cgtgtccttg tagtggatga tttcatgaaa ggcggcggaa
     3421 cagtcaacgg aatgaagagc ttgattgaag aattcgaatc agaacttgtt ggcatcactg
     3481 tatttgccga atcaaaattc aacggtcatc gcgccatcga tgattatacg tcactgcttt
     3541 atgtcaaaga tgtagatact caaacgaaaa atattacggt tgtgccagga aattattttt
     3601 cagaataaga ggttgtgaaa aagccgctta gtttcgagta gtaagaaaaa ttttgtgaaa
     3661 atggctttcc acattttttc ataaatttgg cttaataccg agaaactggc ttttgaacac
     3721 cgtttatcta gaggttgtga aatcagcgtc ctgtcctgag tattagctca ataaacggaa
     3781 aaattgctcc tcatattttg ccgtttattg agctaatacc gaaggtcagc tgattgaaaa
     3841 acgtttaatg aagaggttgt aagcctaccg tttaactcta agtcacaaga aacgatttca
     3901 aaaaacagct tttcatgttt tatgagtagt gtgacttgtg accgaggagt tggcaggaga
     3961 gcaccggtta ccaaatagat aggcccgtta tggtctatct atttttttat ccagaaaacg
     4021 ctgggataaa agaaaatcac ttgttttgtt gaaaaaatgg gtatactggg ataaactgaa
     4081 atcagcaaac tatatttagg agtgacgcag ttggacgcac gttatgcaat tattttggcg
     4141 gcagggaaag gtacccgcat gaagtcaaaa ttatataaag ttttacatcc cgtttctggt
     4201 aaacctatgg tagaacatat cattaatcgg gtcagtgaaa cgaaaccaga tgaagtgatt
     4261 acgatcgttg gacacggagc agaacaagtt aaagcacaac taggagaacg cagtaaatat
     4321 gcgttacaag cagaacaatt gggaacagga catgctgttt tgcaagctgc ttctttcttg
     4381 gaaggaaaaa aaggaacaac gttagttatc agtggcgata caccattatt gactactgaa
     4441 acgctgaaca atctttttga atatcaccaa ggaaaaaatg caagtgcaac gatcttgacg
     4501 gcacaagcag aaaacccaac aggatatgga cgaatcatcc gtgaccatat tgggatcgta
     4561 gaaaaaatcg tggaacaaaa agatgcaaca ccagaagaag ctcttgtaca agaaatcaat
     4621 acaggaactt actgttttga caacgaagct ttgttcgatg cgttaagcaa agttggaaca
     4681 aacaatgctc aaggtgaata ctatctaaca gatatcatcg aaatcttaaa agaagaaggc
     4741 catacagttg ctgcttatca aacggatgat tttgaagaat caatgggagt caatgatcgg
     4801 atcgcgttag ctaaagcaaa tgagatcatg agaaagcgaa ttaaccagat gcatatggtc
     4861 aatggcgtaa gctttgtcga ttcagcaacg acatacatcg atgcaggcgt agaaatcggt
     4921 ccagacacat tgatcgaagc aggtgtacaa atccaaggaa atacagtcat tggttctgat
     4981 tgtgtcatcg ggtcacattc aaaaatcgta gacagtagga ttgaggatca cgtcgtcatt
     5041 gaaaactcag tcattgaaag cagccatgtc aagaaacatg cagatgtggg accttatgca
     5101 catttacgac caaaagctga aattggcgaa aacgtccata tcggaaactt tgtggaagtg
     5161 aaaaatgctc aaatcggcaa aggaactaaa gtcggtcact tgacttatgt tggtgatgct
     5221 acgctgggag aagaaatcaa tgtcggatgt ggtgttgtct tcgtcaacta tgatggaaag
     5281 aacaaacatc atacgactgt cggcgaccac agctttattg gttctagcac gaatatcatc
     5341 ggacctgtcg aggtagctaa aaacagttcg attgctgccg ggtcaacaat tacagacaat
     5401 attccagaat atgcgctggc aattgcgaga gcaagacaag tcaataaaga aggatatgca
     5461 aaaaaactcc cttatctaaa ctaagacttt tgcaaaatca cttgatttat ttaaaaaata
     5521 tgactactat ttattaggta gaattctaga gttgtgaaaa agaaagtgga ggtccccatg
     5581 tcaaaacatt actttgaccc aagattaaaa atttttgcgt taaattccaa tcgcccatta
     5641 gcagagaaaa ttgcagcagc agtcggcgtc gagttaggaa aatcatctgt tactcaattt
     5701 agtgatggag aaattcaagt aaacattgaa gaaagtattc gtggatcgca tgtctatgtt
     5761 attcaatcaa cgagcagccc agtcaatgat aacttgatgg agttgctgat catgattgac
     5821 gcactgaaac gagcaagtgc aaaaacaatc aacgtagtca tgccttatta tggctatgcg
     5881 cgtcaagacc gtaaagcacg tgcacgggaa ccaatcacag cgaaattagt agcaaacatg
     5941 attcaaaaag ctggtgcaac aagaatgctg actttggacc ttcatgcagt ccaaatccaa
     6001 ggtttctttg atattccagt cgatcatttg atgggtgcgc cacttattgc caattatttc
     6061 ttagaaaaag gaatcaaagg cgatgatgtt gtcgttgttt caccagacca tggtggtgtc
     6121 acacgggcac gtaaattagc ggaatttttg aaatctccga ttgcgatcat agacaaacgc
     6181 cgtccaaaag caaatgttgc agaagtaatg aatatcattg gacaagtcaa aggcaaaact
     6241 tgtgtactga tcgatgatat gatcgataca gcaggtacga tcacattggc tgccaatgct
     6301 ttgaaagaag ctggtgcaac tagtgtgtat gcttcatgta cacatccagt attatccggc
     6361 cctgctttac aaagaataga agattcagct atcgagcgtc tagttgttac agactcgatc
     6421 tatcttccag aagatcgtaa aatcgacaaa atcgatgaag taagtgttgg cggactaatg
     6481 ggagatgcaa tcaaacgtat ccatgaaaac aaaccagtta gtccattgtt tgaaacaaaa
     6541 aataaataat aaataaactc acaaagggga gtccaattgg gctgagattg aatctatttc
     6601 taaacccttc gtacctgtat cggttatgcg agcgtaggaa ttgtgaataa acagctgttt
     6661 gactattttc gaaagctgct tcctcctttg tgactttttt attcaaagga ggaagttttt
     6721 ttatgggaaa aaatagagtt tgggtagaag gaacgattgt tgcagcgtta gcgatggtgt
     6781 tgtcgttgat tccgatccag atcggcagca gtttttctat ttcattaggg cagattccat
     6841 tgactctttt tgcgttaaga agagggacaa aggcagggat gctggcaggt ttcatttggg
     6901 gcatcctaca ttttccgttg ggacaagttt attatttaag tgtcgttcaa gtgttgattg
     6961 agtacccgat tgcctatact tttgcaggcg cagcagggct catggcttca aatgtccaaa
     7021 aaggctatgt agacgataat acgaaacaaa ttagaaaaag catcgtgttt ggtgcattga
     7081 ttggcgcact ttccagatat ttttggcatt ttattgcagg agtggttttc tggggagcgt
     7141 atgcactatg ggggatgaat ccttggctct tttctttcgt aatgaatggt gccagcgggt
     7201 tgagtacagc tattgtaacg atcatcgttt tattagggat ccaacaaacg actccttcga
     7261 tttttgtgcc agataaacga caggagctgt aaataaaaaa gaagcaagaa acttgagaca
     7321 gaatcatgtc ctagtttctt gtttcctttt tattttttga taagaaatag atctacttca
     7381 atggcagata agaaagatcg gcatcttcta ccaattgcca gtgtcgatcg gtgacagagt
     7441 agattaaagt agcgatgatt tcctcttctc gataccgggt gccttttaat ttccaaatat
     7501 aatcttcaat cacatagatt gggatacgat atttaccatc gatcaaacca acttgatcaa
     7561 aagaaaaaat gatcccttcc tgcatcaaat tacgcatttc tttcaataat tccacactga
     7621 ttttttcaac aggtgtttct tttttgaaaa aagatttttt tacattgatc ttatctgaca
     7681 agatcgaact taatttacta gcaaaaatcc tataaaattc atctaaatag cgataataaa
     7741 tccgattcag ctgatcatta agatcgaagt atacaaaatt attctgtagt ttataaaaaa
     7801 acggcgaatg aagatgtgtt ttcatatgac caaaatataa taattctgaa atttctaaag
     7861 gtgtcaattc tttcagcatc gttaaatcag taaagtcgat ccacttcaat tcatcgaatc
     7921 cacgcctgtt acgagaagag gaaaagaaac gctgaatatt ttctgtacca cggattactt
     7981 tcagccctgt atgggattca tattctccag cagaagcaga tggatcaagc aagagcaagt
     8041 tttgaggaaa atgcacgata ctgtttgcaa agtcagtagc agtcagccct tttgttaaaa
     8101 cagcattact tgtggtatca atatgtacat aaactgactc agtcatcatg ttcctcctct
     8161 tatttcctct taatctcttt tattttacaa catttgcatt aaaaaagctt gtttctttta
     8221 ttttaaaatt ccattacaaa atcacgtttt cattgaaaac actctcattt tcaattaatt
     8281 tttgtgattt ttgtattttt ttatttaaag gaggttttat gaattgtttt cttatggaat
     8341 ctacaaagtc gaatacgaga ctcatgacct ttttatgacg tactggtata cttgaagaaa
     8401 aaggagagcg cgatggagat aaaaaaagtt tacttttcac agaaaatgtc gctgtattcg
     8461 cctatccagc aagcaaaaca accccttcca ttttttcaaa gtcatcaaga aagtagagac
     8521 gctttttggc gttctgcaaa aaagcaatgt gcttttaaag cagtatcgaa aaaatatacc
     8581 gtctatttta ctattgctaa aagtagagga gatgttattt ttaataatct gttgattgaa
     8641 ggtgaaactt ttgaatggaa aaaatttttt cggtatatgg aagcttgcgc tcgttttttt
     8701 attaaagaaa actgttgttt tttatttcaa tctcctctat cagaagaatg gttgcgtatt
     8761 tttcataaaa acggctatca aggaaccaca caattaaata aaaagctggt ctatcataca
     8821 gcacttgttt taggtggagg aggagcgcac ggtgcttatc agattggtgt gtggcaggcg
     8881 ctgaaggaac atgatatcaa ctttgagatt atcacaggta cctcggtagg tgcgttgaac
     8941 ggcgcattga ttttacaagg agatatgaaa aaagctgtga atttatggaa aaagctgtcg
     9001 acaagacaag tgcttgcgtt accagaaatg gctgcagttg aagaccttcg tgaacgcttc
     9061 tatcgagaaa gacgccaaat gacgaaaaca gcactaattg aaggcggggt ttcttctgct
     9121 ccattggaaa agatggtcaa agacaatttg gatttgtcct tactcaaaca taatcccaaa
     9181 atacgtttat atacggtgtc tactaaactg ccagaactag cggaagtagt gactgatatc
     9241 caacagacaa aaacagaaga aataccagat tggatactcg cctctgcttc attttatccc
     9301 gctatggctt atcgaaagat cggaaaaaat aaatatgcag acggtgggta tcgcaacaaa
     9361 atacctattg atattgcgat taacaaagga gcaacagagg catttgtcgt agatgtgcaa
     9421 gggccaggcc cagcgaaaaa aattcgtatg ccagacattt ttatccattg gaaatgtcaa
     9481 acattgtgga cactaggcag ctttctgctt tttgatagcc aaaggaacca attaaattta
     9541 caattaggct atctggaaat gaaaaaacga ttaggctatt actatgggaa ctggtataca
     9601 tttcattctg ttaaacctgc aagaacatac tggcgaggat ttttgtctta cttaacaaaa
     9661 gaaattcagt tagaattatc ttttttcaaa agcataaaat tttggcaaaa actgcgtaat
     9721 ttatataaga acagggtcgt accagagaca tgcggattgg cgatgctaga acttttagca
     9781 aaaaaacatt ttttactgcc caatgaaatc tatcaagtag acatgatgat tcaaatgatc
     9841 tgccaacaag atataaaaag tatgccggat gatttattgg cacaaattgg tcaattaagt
     9901 actgaagaat ggcgaaggta tcgaaaatac cagcaaaaaa tacaaaatga gcgcacgaaa
     9961 actgtttatt ttgattatct gctacaagga aaacgcaagg atcgtttgca gcatgaattg
    10021 cttaagcaac cagttgacac attactgatc ctatatttat attatctcaa ggaggaacaa
    10081 ccatggcaca agaattttca tatgaaatcg tagaagaaat cgctatattg tcagaaaaca
    10141 acaaggggtg gcgtaaagaa ctgaacctag tcagctggaa tggtcgcccg ccaaaattcg
    10201 atcttcgaga ttgggcgcca gaccatgaaa aaatggggaa aggcctgaca ttgacgaacg
    10261 aagagtttga acaactgcaa aaagcaatag aaaatatgta atgaggaagg accctgtcga
    10321 atgaaaagta tgactggttt tggcaaagcc atccgcgaaa caaaagcgta tcagttagat
    10381 attgaaataa aaagtgtcaa ccagcgtttt ctagatgttc aaatacgaag tcctaaaatc
    10441 ctgaactttg ttgaaaacga ttttcgtcag cttattaaac agcagttaag ccgcggtcga
    10501 atcgaagtat ttgtgaatct agcttatatt ggcaaaaacg aaaaacagat cgtcatcaac
    10561 tgggaattga ttgattcact tatcactcaa ttgacagacg gaattcaaca acgatacggt
    10621 agtcaaaatc aagtgtcaat tagtcggatg gcggaacgct tcgtgctgga cgaaaacttc
    10681 gtaacggtag aagaaaaacc ggaagagagc atggaagaat tggcacagtt agctctagat
    10741 acattgaaaa gcgcacttga ttctattgag gcgagtcgag aaaaagaagg tcaagcttta
    10801 gctttagtgt tgaaaaagaa tactgaagaa ttcaaagaag tactaaaaaa actcgatagt
    10861 tttgtagaaa tctatgaaaa ggaatttcaa ataaggtatc aaaaaaagct ggaggaattt
    10921 cttggagcga aagtcgatga gcaacggttg ttgacggagc tagccatctt gttggaacgc
    10981 ggagatattc acgaggaact ggatcgtctg gcgatccatg tacaaaaact agatgaactt
    11041 atcgaagcac aatgtcctgt aggtcgggaa ttagatttcc tgatccaaga aatgaatcgg
    11101 gaaatcaaca cgatcggttc caaatcaaat gcaatcgaaa ttaaaaatca agtcgtccag
    11161 tctaagacga tcttagaaaa aatcagagaa caagtacaaa atattgaatg acgaaagagg
    11221 tagagataat gaaatattat gacgtaacgt tccacgaatt aagtggaaaa actgtggtta
    11281 aacgggatat cccttctgaa aaaaacggct ttgacgtatg gaaagatgcc tgtgcagact
    11341 ataatgagaa cgaactgttt attttgatca acgacggtgc ttacgtgacg atgaatcgga
    11401 aatttattgt tcgaattgac acagaagagg tagaagatcc gacagaaaaa gcacgttctc
    11461 gaaaagatga gatcatgggt gtagtgaata ctttgtccaa tatgggcttt tagcaaggat
    11521 gctgaacaaa gaaatacaat aaataaacag aattggattt cttcgcttag cttctttgct
    11581 ctaggaatac atttaaaggt tgtgacaaaa ttcgtgtcac aatctttttt tattttgaat
    11641 aagtgatgtt caaaaaccat tttctcggta ttaagccaaa tttatgcaaa aatgtgagaa
    11701 actattttca caaaattttt cttcatactc gaaactaatc ggcttcttca cgacctctta
    11761 tacacggcgt tcaaaagctt gcatctgcga taataacccc aaccaaacga aaaacatgag
    11821 aagctgttta tgtttggctg ttcttattac ttgatgcagg acagcttttt tacgacctct
    11881 tggtacaggg tagctttttt accaatttct agctataatt aaagataacc ttgaaagttg
    11941 cagaaaaaaa gtgcaaaata aacgataagt acaaaaagac tagttgaaag gacgtcgtca
    12001 tgtcagagcg tggattatta atcgtgttgt ctggtccatc cggtgtagga aaaggcacag
    12061 tacgaaaagc aatttttgag agtgaggata atgattttca gtattcgatc tcaatgacta
    12121 cccgtaaaat gcgtgaaggg gaggtcgaag gcgttgatta tttcttccga agcaaagaag
    12181 agtttgaatc aatgatcgaa gctggagaga tgctggaata cgcagaatat gtcgggaatt
    12241 attatggaac tcctttgtca tatgtcaaca agacattgga tgaaggaaaa gatgttttct
    12301 tggagattga agtccaagga gcaaaacaag tcaaagaaaa agtgcccgat ggtgtattca
    12361 tctttttgac tccgccagat ttagcggaat taaaatcacg gatcgtaggc cgaggaacag
    12421 atgcagatga agtaatcgaa aaacgaatga aagttgcaaa agaagaaatc gaaatgatgg
    12481 ctctttacga ctatgctgtt gtaaatgatg aagtgccgct agcagttgaa cgaatcaaaa
    12541 acattattgc cagtgaacat tttcgagtag atcgcgtaat cggtaaatat attaaaatgt
    12601 taaaggagat gtagtcttat gatgttaaaa ccttcaattg actcactatt agatcgtgta
    12661 aattcaaaat attctctagt gattttagca agtaaacgcg cgcatgaatt ggatgcaagt
    12721 gcacagccta ctctagattc ttttgattca gtaaaaagcg tcggtcaagc tttagaagaa
    12781 atcgatgcag gaaatgtagt caatgatcca catcctgaat taaaacgtga acgtttgaag
    12841 atggaagaag aggaacgtca agcacaaaaa gagcgtgaac aacacgaatt ggaatcacgt
    12901 atccgtgaag agcaaaaaat gtaataaaga aaaatagagc gaacatctaa aaagaaaagc
    12961 aaacccagct gtttcctttt caaacattcg ccacttattg tctggtagaa aaggctgtgt
    13021 ctgaactttt tgacacagtt cttttttttc ttatctgctg ttaatttttt gttaggattt
    13081 ggcagaagat aagacaaatc catcgatctc tagtaaaata ggcaaggaac tttttgtcgc
    13141 ttgataagaa aagacaaaaa aaggaggaaa cgaaccatga cattaacagc tgaagtcatt
    13201 gtggacgtac ctactatgca gaccgatcag ccattcactt atttggttcc ctctgaaatg
    13261 gaaacagctt tgcaagtcgg gatgcgagta gaagtccctt ttggtaatgg caatcgtcat
    13321 gtccaaggat ttgttatggc tatccaaaaa tcagaagaat cagctaatcc atctttgaag
    13381 gcagttattc gtttgctaga ccttgctccg gtggtcaatg aagaattact ttcattagct
    13441 gattatatga aaaaaattac ttacgcattc aaaatcactt gtctacaaac gatgcttccg
    13501 agtgtgatga aagcagaata tgataaattg atctatcctt tggcagacac accagaagtt
    13561 caggtattgt ttgatcagcg agaagtgatt tcctggaagg aagcagaaga agaaggcagc
    13621 ctttctcaat tgattcgctg gcgccaagag caattagtag atatcaaata tgaagtccat
    13681 acacgaaata aagtaaaaac gattcgtttg gtccgttctt tgttaactga aaagcaaatc
    13741 gaagaagaat gggcaaaatt acggcaaaat gccaagaaac aaaaagaact tcttttgtgt
    13801 ttatctgaac tgagccaaga agaaccaatt gcttatttca aaaataaagg aatcagtact
    13861 gctgtcttga atcaaggaaa agaaaagggc tggctggagt ttatagaatc agaacgttat
    13921 cgcgaccctt ataaagaccg agtatttgac caaacgacgg cattggaact gaatgcagaa
    13981 caaaaaaatg ctgtcgaaca aatcatcaca gcaggtcagc agcaaaaaga tcaggtcttt
    14041 ttgctggagg gaatcactgg cagcgggaaa acagaagtat atcttcaggc aatcgctgat
    14101 gttttgtcag aaaataaaac ggctattatg ttagtgcctg aaatcgcatt gactccgcaa
    14161 atggtcgagc gattcaaagg acgcttcgga gaatcagttg cggtcttaca tagcggactt
    14221 tcccaaggtg aaaaatatga tgaatggcgc aagattgagc gggaagaagc gcaagtagta
    14281 gtaggcgcac gatctgctat ttttgccccg ctgaaaaata tcgggctgat cattatcgat
    14341 gaagaacatg aatcaagtta caaacaagat gagacaccgc gttatcatgc gagagatcta
    14401 gcaatttggc gcagtaaata tcatcattgc ccaatagttc taggaagcgc gacgccctct
    14461 ttagaatcaa gagcacgagc tcaaaaaggc gtttatcagt tattgcagtt aaaccaccga
    14521 gccaaagcag ctgcacagct tccagctatt gagatcgtag atatgcgtga agagtttcag
    14581 aatcatcgga cctctacttt ttctgctaat ttgcaggaaa aaatacaaaa tcgattagac
    14641 aaaaaagaac aaactgtcct actgctcaat cgtcgaggat attcgtcttt tgtgatgtgt
    14701 agggactgtg gatttgttct tccatgtcct aattgcgata tctctcttac actgcatatg
    14761 gatacacgtt caatgcgctg ccattattgc ggacatgaag aacctattcc caatcgctgt
    14821 cctaattgtg gcggcaataa aatccgctac tatggaacag gtactcaaaa agtagaagag
    14881 gaactccgag aactttatcc gcaagctagg attttgcgta tggatgtaga taccactcga
    14941 agaaaagggg cacacgaaca gattttacaa aaattcggtg caaaagaagc agatatatta
    15001 ttagggacac aaatgattgc taaaggtttg gactttccag aagtgacatt agttggggtg
    15061 ttgaatgctg atacctccct gaatttaccg gattttcgtt ctagtgagca tacattccaa
    15121 ttgcttaccc aagtttcagg acgagcaggg cgagcggaaa aagcaggcga agtcgtgatc
    15181 caaacattca atcctcaaca ttacgccata gaattggcta aaaaacaaaa ttatgagcag
    15241 ttttatcagc aggaaatgca tgtacggcat cgtggaggct atcctcctta ttactttact
    15301 gtgaaaataa ccgccagcca tcctgaagaa caagtagcgg caaagaaaat ctttcagatt
    15361 gccaatcagc taaaagaagt gttaagccct caaagcttgc tattagggcc aacgcccagc
    15421 atgattttga gagtgaaaaa ccgatattat tatcaactaa tcatcaaata taaacatgaa
    15481 ccgaatcttc ctcatgtttt ggatgaaatt ttgaatggaa gccagaaaga acagcgacaa
    15541 ggtctttttg tggcaattga caacgaacca ttaaacttta tttaagaaag ggccaaatta
    15601 tgcgctatcc tatattgatc caccctaatg ataaattaaa aagaactgct cagccaatcg
    15661 atgtaatcac ggacgagaca atcgctttat tagacaattt atatgagaca atgattgcga
    15721 atgacggaat agggatcgct gctcctcaag taggacaaaa caagagaatt gctgtgatcg
    15781 aagtagatga aggcgagaaa tttgaactga ttaacccaga aatcatcgaa gcaaaaggtg
    15841 aaagccttga cgtagaaggt tgtctcagta ttccgcatgt ctatggaacg gtcaagcgag
    15901 cagatgaagt gaccgtgcgt tattatgatc gagacggaga agagatagaa gtgacggctt
    15961 ttggctattt agcccgtgcc ttccagcatg aaatcgatca cttagacggt atcttgttca
    16021 ttgagaaaat gatacagcag atcccggaag aagaacttga agaatatatg gaggaacatc
    16081 ttgatgacta agctagtatt tatgggcaca cctgcttttt ctgtgccgat tcttgaaggg
    16141 ctgatagaat caggttacga gatccaggca gtggtaacac agcccgaccg tccagttggg
    16201 cggaaacgag taattacgcc gacaccggta aaaaaagcgg ctctgaagca cggtatccgt
    16261 gtgctgcagc cagagaaaat ctccggttcg ccagaaatgg aagaaatcat tgaattggtg
    16321 cctgatgtga tcgtcactgc ggcatttggc caattcttgc ctgaaaagtt attgcaagta
    16381 ccaaaattag gggcaatcaa tgtccatgct tctttacttc ctaaatatcg gggaggtgcg
    16441 cctgttcatt atgccatcat gaatggggag aaagaaactg gtgtaacgat catggaaatg
    16501 atcaaaaaaa tggatgcagg cggaatctat gcgcaagaaa gcataccaat cactaaacaa
    16561 gatgatgtag ggacgatgtt tgaaaagctt agccttttag gaagaaagct attgctagaa
    16621 acattgccca atattttgga tggacaaaaa ccagtacctc aagacgagtc agaagcaact
    16681 ttttcgccaa acatcacacg ggaacaagaa gcaatcgatt ggcataaaac tgcagaagaa
    16741 attgacaacc aagtacgcgg aatgcgtcct tggccaattg cattcacgac ctatgagggc
    16801 atacgctgga aattattatc ggttgaagta ttggcagaaa ctacagacaa agcacctggt
    16861 acgatcatcc ggaaagacaa aaaaaatcta tggattgctt gcggtaaaca gaccgtctta
    16921 gccatcaatg aattacagcc agctggtaaa ggaaaacagg ccgtacatga tttccttaac
    16981 ggaagcggtc agcaagttca gattggtcag caggtgaaat aatgaaaaaa atccctaaaa
    17041 aaatgaaaaa atcagtacga tatatggcat tactgacatt agagcgagtg aacaagggtg
    17101 gcgcctactc gaatcttttg ttgaatgaga tgatcaaaca aagcgagcta aatcaaaaag
    17161 atgtgggttt atttacagaa ttgatctatg gtacgattag ccatcagcga ctattggaat
    17221 tttacgttgc accattgatc gctaaagcaa aaaaagtgga tgactgggtc aagatgcttc
    17281 tttatctttc tgtataccaa ttagaatttt tagataaagt ccctgcacat gctattttaa
    17341 acgaggcggt cgaaatcgca aaagtaaaag gaaatcctgg gacagggaaa tttgtaaatg
    17401 gtgtcttgag gaattatcag cgtcaaggag caccagatct gcaaggcatt gctgatccta
    17461 tcgaaagact ttctgtcaaa atcagcctgc ctctatggat gacgaaacga ctggtggatc
    17521 aaatcggata cgaagaaact gaaaagctcg gtttatcgct atatcagcct agccatgcta
    17581 gtgcccgagt agatacacgc cgactttcca gagatcaggc catcactgtt ttgcaagaag
    17641 aagagatcac agctgaaaaa agccaagtca gtccatacgg tatagtagcg aaaaaagggc
    17701 atttagcttc tagctcattg ttccacgatg gcgtcatgac gatccaagat gaaagttcga
    17761 tgcttgtagc gccgtctatg caaatcgagc ctgaacatca tgtcttagat gcctgtgcag
    17821 cacccggagg taagacgacg catattgctt cttttttaga tccgtcatgc ggtggagaag
    17881 tggtcgcttt agatgtacac gaacacaaag tcaaactcat tgaagataat gcagcacgtc
    17941 taggtgtctc ttctgctgtc tacgctcaaa agatggatgc tagagaagtc agtaaacagt
    18001 tcgaagcaga gcagtttgat cggattttag tagacgcgcc ttgttcaggt cttgggctgc
    18061 ttcggcgaaa accagatatt cgttataaaa aacagccgga tgaattagcg aatttaccaa
    18121 aaatccaatt ggaaatttta gaaagctgcg caccaacttt aaagtcttcc ggtattctga
    18181 cttatagcac ttgtacgatc ttaaaagaag aaaaccaaga cgttgtttct gctttcttgg
    18241 aaagacaccc tgattttgag aggattgatg ttttgaccaa tcctatgcta caatcatcaa
    18301 tcgaagagaa aatgttgact ttgtatccgc atcagtttta cacagatgga tttttcattt
    18361 gttgtttgcg caaaaagtaa tgaggtgaaa aaatgcagat tgaatatcaa tcagatgtag
    18421 gaagacgacg taacacaaat caagactatg ccagtgtatt taccagccgt tcaggtctca
    18481 agcttgctgt attagcagac gggatgggtg gtcatcgtgc cggagacatt gccagtcaga
    18541 tggcggtaac gaatctaggc gaagcttggg aaaaacaaga agctgttgac gatgaaaaaa
    18601 tcgcccaatg gttcattcaa tcgattcaag aagaaaatat gctgatttat cagcgtggac
    18661 aagaacagcc ggaatataac ggtatgggaa caacaatcgt agcggccgct ctgtcagatg
    18721 actggttcac gattgccaat gttggggata gcagagctta tttgattcgt gaagataaaa
    18781 tcatgcaatt gacggaggat cattctttag tcaatgaact agtcaaatca ggagaaatca
    18841 gcgaagaaat ggcagtgaac catccaagga aaaatatttt aacacgttct gtcggcatgc
    18901 ctggaacagt cgaagtagat gtttctactc atctttggca actaaaagat cgactgctgt
    18961 tatgttcaga tggcttgacg aacatgctgt ctgaagaaga aataggaacg atcatcaatc
    19021 aagaaggccc attgtctcat aaagtaactg agctgattga tcgtgccaat gaagcaggcg
    19081 gagcggataa tatcaccgtt cttcttatcg aatataagga ggacagtgta tgatcgagtt
    19141 gggcaaaaaa ttaaatggcc gttatcaaat cactggaaat atcggtagcg gtggtatggc
    19201 aaatgtattt ttggcgcacg atttaatctt ggatcgtgat gtagcagtta aagtcttacg
    19261 atttgacttt caaaatgacc agactgcgat tcgacgattt cagagagaag ctttagcagc
    19321 aacagaactc gttcatccaa acattgttag tgtttacgat gttggtgaag aagacaacat
    19381 gcagtatctg gtgatggaat acgtcaaagg aatggacttg aaacgatata tccaaacaca
    19441 ttatcctgtt ccttatgaaa cagcagtaaa tattatgcag cagatccttt ctgctatttc
    19501 tttagctcat aaccatcaaa tcatccaccg agatttgaaa ccccaaaacg tactgatcga
    19561 taatgaaggt gttgtaaaaa tcacggattt cgggattgct atcgctttat ctgaaacatc
    19621 gatcacgcag acgaatacga tgctaggatc tgttcattat ctgtcccctg agcaggccag
    19681 aggaagtatg gcaacgaagc agtcagatat ttatgcttta gggatcatcc tttatgagat
    19741 gttgacgggt agcgtaccgt ttgatggtga atctgctgta acaattgctt taaaacattt
    19801 tcaagatgat cttccatcta taaaagcact tgatccaaat gtgccacaag cactggaaaa
    19861 cgtgatttta cgggcaacgg ctaaagaacc tgcagaccgt tataagtcag ccgaggaaat
    19921 gtctgatgat ctttctacag tattatcacc agctcgtgca aacgaagaaa aatggcagcc
    19981 tcatgtgatg gataatgaaa caaaagttat cacacccctt acggaagatg caccaatgcc
    20041 tgattcattc aaatctatgc ctctgccaaa agacaagcca gaaaacgaaa ccttaccaga
    20101 ggaggaagaa gttctgcctg atcctaaaaa gaaaggacag aaaaaatggt ggctgatcct
    20161 attagctatt cttgctgttt taggaatcgg aacagctgta ttctttgctt ctggcgggcg
    20221 aggagaagtt gttgttcctg atgtttctga tctttctgaa gcttctgcta gagaaacact
    20281 cgcaaaagcg aaattagaag tagcaacaaa aacagaagaa attgcagatg acaaaatcga
    20341 agaaggtaat gtagtgaaga cagacccggc agcgggaacg acagtcaagc aaaaacgcga
    20401 agtcactctg tatattagta gcggcacgaa aaaaatcaaa ttagatgatt acagtggtaa
    20461 aagttatgaa gatgccgaag caagtttgaa agaattaggg ttttctgctg acttgattca
    20521 aaaagaagag gaattcagtt cagaggtaga aagcggaatg atcatttcgc aatctccatc
    20581 agaagataca gaagtcgatc ctaaaacaga cacgattact ttcacagtca gcaaaggacc
    20641 agaaacagtg actttgagta gtcatgtagg agaaaactac gaaactgttc gtaattggct
    20701 tgttacacaa ggattcaata tcaataatat tagtgctact tatgactata gcgacactgt
    20761 accatcaggt tcgatcatca gtcaggatcc agcctcgggt gaagttgtag cagaagaaac
    20821 ttacatcaat tttgtcgtaa gccaaggaac agagcctaaa ttgaatgata tctccggcta
    20881 tacaaaaagc gaagcacaaa gttatttagc tagtgtcggt gcagaatata tcgggcatga
    20941 gacttccgaa tattcaaaca cggtggataa agataaagtg attcgtacag taccaggggc
    21001 aggaacgaca attaccaaag gaatggttgt gaacgtaatt tactctaaag gaccagatcc
    21061 agcgtcttct agttctgaaa aagactcttc agacagttct agcagctcaa gtagctccag
    21121 tagcacaagc aactcttcaa aagaagaatc ttccagctcc acaagttcat cagctagcac
    21181 ctcagaaagt tcaaaataag tgctgaataa aatatatcag aagggaatgc gacattgatt
    21241 tgttgcattc tcttttttgt gtttctatct cttttgatca attttcaagc catatacttt
    21301 gagaagtttg catggaataa cacctacttt cctcttcgta tggtaaaata aacaaaaacg
    21361 gaggtggcac ttatcaaagg acaaatcaga aaagcaatca gcggttttta ctatgtatac
    21421 gcaaacggag agacctatca aacaagagga agaggaaatt tccggaatcg gaaaatcacg
    21481 ccgcttgtcg gagatcaggt gatctttgag agtgaaaatc aaacagacgg ctatcttctg
    21541 gaaatcttgc cgagaaaaaa tcaattagtt cgcccaccag tcacaaatgt tgatcttgga
    21601 atcattgtca caagtatcgt agagcctgca ttttcttata atctacttga ccgctttcta
    21661 gttaccttgg aatacgagtc gatcgaaccg attatttttt tgacaaagac cgacttgtca
    21721 gacgacccag aaaaaatcga agagatcaag caaacctata ctaaaatagg ctatccggtg
    21781 attgtaccca atcatacggg tgatacgaca gaactgatcc gttacttccc agaacggcta
    21841 tcggtcttta tgggtcagtc tggagcagga aaatcgacgc tactgaatca aatatcaccg
    21901 gaactgaatt tagcaacagg agagatttct gatgcacttg gaagaggacg gcatacaaca
    21961 cgtcatgtgg aactgattcc tatctatggc gggttagtag cagatacacc aggatttagc
    22021 tcgattgact ttttgacaat tgaagcagga gaactgccga gacaatttcc tgaatttgta
    22081 gaagcttctg tccattgcaa atttagagag tgcatgcatg ctaaagaacc tggctgcgaa
    22141 gtaaaacata gagtagaaac aggtggtatc gcacagacac gttacgacaa ttatttgcaa
    22201 ttcttacaag aaatcgaaaa aagaaaacca atgtatgcaa aaaaagaagg taaacgaaaa
    22261 taggaggaaa caagaatgaa gatagcacca tccattttga gtgcagattt tgccaattta
    22321 caaagagaca ttgagttagt ggaaaaagga ggcgcagatt acatccacgt agatgtaatg
    22381 gatggccgat ttgtcccaaa tatcacatta gggccaaata ttgttcaggc aatccgtcct
    22441 gttactaaat taccgttaga cgtccatctg atgatcgttg atccagaaaa ctacattcca
    22501 gcatttgcaa aagctggagc agatatcatt actgtccacg tcgaagcaac tcctcatatt
    22561 catcgggcac ttcaaatgac aaaaagctta ggagttaaag ctggagtagt tatcaatcca
    22621 ggaacaccag tctccatgat caaacatgtt ttgcctttgg ctgatcaagt actagtcatg
    22681 actgtcaatc ctgggttcgg cggacaaagc tttattgaag aaacagtaga aaaaattacg
    22741 gaactcgctg atctacgtag agagaacaat tggacttatg caatcgaagt agatggcgga
    22801 atcgttccag aaacagcaaa aatctgtaaa gatgcagggg cggatgtctt cgttgcagga
    22861 tcatatatct atggagccga aaatccagtc ggacaaatcg aacgattaaa agaggcatta
    22921 gactaaatga aaattttatt agctgctgga ggtaatccgc aaaactggcc gcagtctctg
    22981 ctgtccaaat acgatgccta tgtcgggata gaccgtggtg cgctgtacct gcttcaagca
    23041 ggctacccag tagatctggc agtgggtgat tttgattcat tgtcgaacga agagcagaag
    23101 gaagtatttg caaaagccaa agagtatacc cgttcccctg cagaaaaaga cgatacagat
    23161 acacagttag gtatcttaaa aacatttgaa cgttacccgc aagcggaagt cactttgatt
    23221 ggagctacgg gcggaagatt ggatcatctt ttagccaatc tctggttagg tatggaacct
    23281 cgcttcaaac cctttgccca tcagcttatt atcagagatt gtcaaaatta tttgtcttat
    23341 tatttgcctg gagagcatat agtaagacag ataccagata tgaagtattt agcttattgc
    23401 tgcttgactc ccgtctcgaa tcttacatta aaaggcagca agtatgtatt ggaaaataaa
    23461 gaagtagaat atccgacatc ttatgcaagt aacgaatttc tttcagatac cgcagaattt
    23521 tctttttcag atggtatgat tgcagttatt caaagcaaag attgatccat aaacaaataa
    23581 aaaaatttgt ctttcaggct aaaaatgaca gaatctatta tttgctaaaa aaaagcttaa
    23641 gaggagcaac ttaccttttg tttcacggca tttagtgata aaattgaaca caagagtcta
    23701 attaattcga ggtgtctttg tgaagaagtt caatcctaat aaaaatatta ttattacctt
    23761 ggttattgtt atcatcgtgg tgacgatact aagtgtgacc gctgcgcagc gagcgaatga
    23821 aggaaagccc aatttcttcc aatccatcgt taatgacagt gttgcaatgg ttgataaaac
    23881 aatctctgcc ccagtacggt gggtacgaaa tggagtagat tctgtccata atcttttttc
    23941 aacttatgcc gaaaatgagc ggctaaaaga aaaaatcgat agctatgatg acgtttcaca
    24001 gcaaaacaag aattacgaga gagaaatcga agcgttgaaa aaagaacttg agttgaatca
    24061 gacgctgact agttacgaaa aagtaacggc aaatgtcatt actcgttcgc ctgacagttg
    24121 gcaggacatg ctgatcgtag acaagggttc taaagacggt atcgaagcaa atatggcagt
    24181 catgtcgcaa aaaggattga ttggccgagt gatcgaggtc aatacggctt cgtctaaaat
    24241 cgaattactg tcatcctcta atgaaagctc caatcatttt ccagtacggg tatcttcggc
    24301 taatggcgaa gcgtttggtt tgcttaaaaa ctatgatgaa aagctccatg ccttagtggt
    24361 gacccaatta actggtgata cggatatcaa agaaggggat gttgtccaga catccggtct
    24421 tggagggaat tctccagcta acttgccgat cggtacggtt attaaaacga aaccagatag
    24481 ttatgggctg gatcgggaag tttatgtgaa accttatgca gaaatgtatg acgtgtcagt
    24541 tgtgacgatt gtccaacggc tagcggagga ttagtccatg ttgaagaaag aaacaatgaa
    24601 gtactatctg ccaatcgttt tgttcttttt gatgttgata gatggtcatt taacaagaat
    24661 gctaggggag tggtcgaaag gcacctatat gtcaaatgcc cactttctga tattggcatt
    24721 attatgttgc agtatggcgt ttgaaaaacg ttatttactg attaccacga ttgttctcgg
    24781 ggctatctat gacgcttact atattggcgt tatcggtatc tatgcagtag ctctcccttt
    24841 aattgtatgg ttgatgtatg taatgaaaga cgttatccat gtcaacatct ttactgaatt
    24901 tttcagtatg atcatctttg tcacgggtta tgaattgttt acgatggtgg tccagttgat
    24961 ttttaaatta gcagtagtaa ataacacgta ttttattaca aggtttttag gacctacact
    25021 gctgttgaac atgattatat ttgtattatt catttttccc tttaagaaat tattcagtga
    25081 tgaataattt ctttttttat ttttaggtcg aaagcgaaaa agaatcgttg ttatcttaag
    25141 aaatgctaga cttaacgcgg ctttttcatg atcattttct gaaacgaaca gttttgtcat
    25201 ttttaaaagt tgttttgaaa catttttgta acataactat tatatcgttg acacactgct
    25261 atggtacaat ttttatatcg ttaagaaaga cttaaatata tttagatatt tattaaatta
    25321 agccagtcgg aggaatgaag agtgaaaaag agtttaatat cagcagtaat ggtatgttca
    25381 atgaccttga ctgccgtagc atcaccgatc gctgccgcgg cggacgattt tgattctcag
    25441 atacaacagc aagatcaaaa gattgcagac ttgaaaaatc aacaagcaga tgcacaatca
    25501 caaatcgatg ccttagaaag ccaagtttct gaaatcaaca cacaagcaca agacctatta
    25561 gcaaaacaag atacattgcg tcaagaatct gcacaattag taaaagacat tgcagattta
    25621 caagaacgta ttgaaaaacg tgaagataca atccaaaaac aagcacgcga agcacaagta
    25681 agcaacacaa gctcaaacta catcgatgct gtattgaatg ctgattcatt agcagatgct
    25741 attggacgcg ttcaagcaat gacaacgatg gtaaaagcaa acaacgactt gatggaacaa
    25801 caaaaacaag ataaaaaagc tgtagaagac aaaaaagctg aaaacgatgc taaactaaaa
    25861 gaattagctg aaaaccaagc tgctttggaa tctcaaaaag gcgatctttt atctaaacaa
    25921 gctgatttga acgtactgaa aacttcatta gctgctgaac aagcaactgc tgaagacaag
    25981 aaagctgact tgaaccgtca aaaagcagaa gctgaagcag agcaagctcg tattcgtgaa
    26041 caacaacgtc tagcagaaca agctcgtcaa caagctgcac aagaaaaagc tgaaaaagaa
    26101 gcacgcgaac aagcagaggc tgaagcacaa gctactcaag cttcatctac tgcacaaagc
    26161 tcagcaacag aagaatcatc agctacacaa tcttcaatga ctgaagaatc atcatcagct
    26221 acacaatctt cagcaacaga agaatcaaca acacctgaat cttcaacaga agaatcaaca
    26281 gcgcctgaat cttcagcaac agaagaatca acaacagcgc ctgaatcttc agcaacagaa
    26341 gaatcaacaa cagtgcctga atcttcagca acagaagaat caacaacagt gcctgaatct
    26401 tcaacaacag aagaatcaac aacacctgcg cctacaacac catcaacaga tcaaagtgtt
    26461 gatacaggaa acggcacagg aagtagtact ccggctccaa cgccaacacc aacacctgaa
    26521 caaccaaaac ctgtaacacc tgctccagct ccttctggaa gtgtgaatgg tgcagcaatc
    26581 gtagcagaag cttacaaata tattggtact ccatatgttt ggggcggtaa agatccaagt
    26641 ggctttgact gctcagggtt cacacgctat gtttacatgc aagtaactgg tcgtgacatc
    26701 ggtggttgga cagttcctca agaatcagct ggaacaaaaa tttcagtttc tcaagcaaaa
    26761 gctggagact tgttattctg ggggtcacaa ggcggaactt accacgtagc gattgcttta
    26821 ggcggaggac aatatatcca tgctcctcaa ccaggcgaaa gcgtaaaagt tggttcagtt
    26881 caatggtttg cccctgactt tgctgtcagc atgtaagaat gattaatcaa aaagagttag
    26941 cccattctgg actaactctt tttttattgt gataaaatag aaatcagata aattcttctg
    27001 tcaattccat gaacgtttgg atatcttcag tcaccatgtc gatacctgct tgccaaaaat
    27061 ctggttgggt aagatcgaca ttcaaatgtt tcttcgcaag atcttccgtc gtcattgagg
    27121 cagtatcgcg tagtaaatgg atatattctt cttcaaaatt tgctcccttt tggttggcgt
    27181 aagcatagat gcccaaacta aagaggtagc caaatgtgta tgggaagtta taaaacggta
    27241 cgtcatcaat aaagaaatgc aattttgcag cccagaaata tggatggtaa gtagctaaag
    27301 ctccttgata gccttctttt tgcgcctcaa ccatcatctc tgtgatctta tcttctgaga
    27361 ctaatccttc ttgccgtgct tcatagaatc gattttcaaa aatgaatcgg gaatggatat
    27421 tcatgaacat agctaaagca ttttgcagtt ttgtatctag caaattgatt ttttcttctt
    27481 ttgtttttgc gacttttaat gtagcatcgg ctacgatcag ttctgcgaat gtacttgctg
    27541 tttctgcgac attcatggca tattcgcgat ttaaagaagg taaatcccac atcgtgctgc
    27601 tatggaaagc atgtcctagt tcatgagcaa gtgtcgcaac ctcattaaca gaattactgt
    27661 aggtcataaa gatccttgat tcttttgttt ctggtaactc agtacaataa ccaccaggac
    27721 gtttcccagg acgatcttct gcttcgatcc agctcttttc aaaagcagat tgggcaaatt
    27781 tagccatttt tggactgaat ttgttaaagt tttcgataat aaatgctgct gcttcgtcaa
    27841 aactaaaggt tttttctttc aaatcgccta aaataatagg tgcatcttga tcttgccatt
    27901 ccattttttc tttaccgaaa agattagctt ttctagtcaa gtaattaaca aggggttgtt
    27961 tgtttttctg gattgtatcc cacatgacag acaatgtttc tttcttcaac cgattgtact
    28021 ctaatggttt ttgtagaaaa tctgtgacac catgaagttc atagtcagaa agtctaaaac
    28081 catctaaatg attcaaagta tccgtgaaga ggggtgcctt ttctttccag gcttcttccc
    28141 atgctgcgaa aagtgtttct ctgacttttg gatctggatc gcccatcatc ttattgaagg
    28201 cttgtccagc agataattcg actacttgtc catcttgttc gaagggaatc gaaatgctgg
    28261 ctacaatcgt atcataatga ctgctccaag catttagacc atctaaagaa agcgtattta
    28321 taatgttttc ttcagcttct gataataatt gtgagccatc acgacgaatc tcgtttaaac
    28381 gaaaagcaat tgtttcaaac gaagattgag aaagcatctg attccaatca ttgtcagata
    28441 tttctgcaaa tttctttgac aatacagttt cggctgattg cagacgaggc agcattgcgt
    28501 aaagcttgcc tgataagacc tttgcatctg aatcattgac attggcagaa agcaatgcag
    28561 taatatagct gctgcattgg gtaaaaccat tcgtaatcgt ttcttgcaat tgaagaatag
    28621 cgtttaactg ctctgcattg tcagaactag gtgaccattt tgttacgcgg tgataatact
    28681 cgttcatttg gtcttccaat tgcttcatcc gttggttcaa agcatcagaa tgactgcctc
    28741 cggaaaaaat agaatctaga ttccaattta atgaataact catgaaaaaa ctcccttcat
    28801 attgtaatac aaaattaagt atagcacttt tttatggaag aagtctctat gaaaatgatt
    28861 gttgtttctt caaaaaaagt ctaagataaa atacagaggt gacttatgaa ttcttcaaaa
    28921 aaacaaatga aaaacggaaa tatttattta gtaatcgcac tgatagtcgt tataatctta
    28981 ttttacagtt cttctcaaac ttacggacag caatcacaag tttcgcgatt agaaagctgg
    29041 ctgcctaatc agccatttaa agacacatta gcgggtatac gttttaatta tggtggaagt
    29101 atggtgagta tcaaccatct gggctacgcc aaatttttag agttcttttt aaggaagggc
    29161 gcacatttcg gtacctattt tattttggga ggcagcttgt ttttaggtgt tttccccaag
    29221 ctaaaaattt ggtggctgac agcgatcctt gcttggcttt cagcaacagg gtacgcaggt
    29281 ttggacgaat tccatcagat gatgaccggt ggacgaacac ctatgttcca agatgtcatg
    29341 ttggattcga tcggtgctct gactgctgtg atattatgtg tgctttttgc ggcattcaag
    29401 aagcgtttat agattggctg tggctttgtt tttttgtgaa atagtactag aatagaagtg
    29461 tattctcaaa agaacaaggg aggaaaaatc atgtcaggac atagtaaatg gcataatatc
    29521 caaggaagaa aaaatgccca agatgcaaaa cgagggaaag tctttcagaa actttctcgt
    29581 gaaatttata tggcagctaa agcaggcggt cctgatccat ctatgaatcc agctttgcgg
    29641 cttgttgtgg acaaagcaaa agctgcaaat atgcctaacg acaatgtgga acgggcgatt
    29701 aaaaaggcta cgagtgccgt agatgaaacg aattacgatg aaatcacgta tgaaggatat
    29761 ggtccgggtg gagtaggaat cttagtccat gcattgacag ataaccgtaa tcggacagcg
    29821 acaaatgtcc gagtagcttt tactcgaaac ggtggatcac tgggagaaac gggttcagtc
    29881 agttacttgt ttgaccgcaa aggatatatc gctattgaac gcgatgggtt gactgttgat
    29941 gaagagacga tgtttgagga tgtattagag gcgggggcag aagatctgca aacttctcct
    30001 gaagtattcg agatttatac tgcaccagaa gattttgctg ctgtacgaga tgagttggaa
    30061 aaagagttca ctttggcgca agcagaattg acgatgattc cacaaacgac gattgattta
    30121 aatgaggaac aaaaagagca attggaacgt ctagtagata agctagaaga cgatgatgat
    30181 gtttcagaag tctttactgc tgctgaaatg taagtagcac attgttagat aataaaaaga
    30241 aagcacctgt accaatcgta cgggtgcttt ctttttatat attatttt
//
LOCUS       DACDXO010000031        27566 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E23605_2017
            SAMN12639989-rid9881463.denovo.031, whole genome shotgun sequence.
ACCESSION   DACDXO010000031 DACDXO010000000
VERSION     DACDXO010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639989
            Sequence Read Archive: SRR10023389
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741914.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:42:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,972
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 33 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 17 of 73
            Pseudo Genes (multiple problems)  :: 10 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27566
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639989-rid9881463.denovo.031"
                     /isolate="E23605_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            476..1339
                     /locus_tag="IWM35_001555"
     CDS             476..1339
                     /locus_tag="IWM35_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAP9919415.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(1468..3090)
                     /locus_tag="IWM35_001556"
     CDS             complement(1468..3090)
                     /locus_tag="IWM35_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP9919416.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            3339..3860
                     /locus_tag="IWM35_001557"
     CDS             3339..3860
                     /locus_tag="IWM35_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9919417.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(4046..5776)
                     /gene="spxB"
                     /locus_tag="IWM35_001558"
     CDS             complement(4046..5776)
                     /gene="spxB"
                     /locus_tag="IWM35_001558"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HAP9919418.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            5941..6759
                     /locus_tag="IWM35_001559"
     CDS             5941..6759
                     /locus_tag="IWM35_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005232914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HAP9919419.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKRHFAVFGVMSGFIIMMILDVALG"
     gene            complement(6862..7059)
                     /locus_tag="IWM35_001560"
     CDS             complement(6862..7059)
                     /locus_tag="IWM35_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9919420.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            7228..7806
                     /locus_tag="IWM35_001561"
     CDS             7228..7806
                     /locus_tag="IWM35_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HAP9919421.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
     gene            complement(8851..9354)
                     /locus_tag="IWM35_001562"
     CDS             complement(8851..9354)
                     /locus_tag="IWM35_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HAP9919422.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            9713..10738
                     /locus_tag="IWM35_001563"
     CDS             9713..10738
                     /locus_tag="IWM35_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAP9919423.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
     gene            11061..11939
                     /locus_tag="IWM35_001564"
     CDS             11061..11939
                     /locus_tag="IWM35_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9919424.1"
                     /translation="MRFKDMKRGNLMKKISNEIQILAETLTLPKVVIEQMIVQAKNIL
                     DEVFEQYSHELMDPNKAKATAKKIDKVYEEEPFAALAIYLYAASQSWKKIYQPLKIPR
                     TIYLATMNAFTRFIHEHFQVTGSYRFDRGFWIWHYTSGLIFRIGELEYEMTYFPKGYT
                     DLRLEGKQCLSLHIPSDADLSQNKIRDSYESAIRFFELYFPDYHYQVMYTDTWLLSPN
                     LTKWLKKESKICLFAADYRLLSVDEQDDSGVPWIFGRVDAQIHDYPESTSLQRQAKEQ
                     LLAGEHIGSGLGILTS"
     gene            12415..12531
                     /locus_tag="IWM35_001565"
     CDS             12415..12531
                     /locus_tag="IWM35_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HAP9919425.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
     gene            13459..13797
                     /locus_tag="IWM35_001566"
     CDS             13459..13797
                     /locus_tag="IWM35_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9919426.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
     gene            13891..14121
                     /locus_tag="IWM35_001567"
     CDS             13891..14121
                     /locus_tag="IWM35_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9919427.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
     gene            14521..14892
                     /locus_tag="IWM35_001568"
     CDS             14521..14892
                     /locus_tag="IWM35_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HAP9919428.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
     gene            complement(15166..15858)
                     /locus_tag="IWM35_001569"
     CDS             complement(15166..15858)
                     /locus_tag="IWM35_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP9919429.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(15861..16646)
                     /locus_tag="IWM35_001570"
     CDS             complement(15861..16646)
                     /locus_tag="IWM35_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP9919430.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            16883..18283
                     /gene="lacG"
                     /locus_tag="IWM35_001571"
     CDS             16883..18283
                     /gene="lacG"
                     /locus_tag="IWM35_001571"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HAP9919431.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            18308..18625
                     /locus_tag="IWM35_001572"
     CDS             18308..18625
                     /locus_tag="IWM35_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAP9919432.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            18638..20311
                     /locus_tag="IWM35_001573"
     CDS             18638..20311
                     /locus_tag="IWM35_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9919433.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSAMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            20325..21956
                     /locus_tag="IWM35_001574"
     CDS             20325..21956
                     /locus_tag="IWM35_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016608433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinogalactan endo-1,4-beta-galactosidase"
                     /protein_id="HAP9919434.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            22828..24018
                     /locus_tag="IWM35_001575"
     CDS             22828..24018
                     /locus_tag="IWM35_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISLgar5 family transposase"
                     /protein_id="HAP9919435.1"
                     /translation="MNDFTTEILKTLANKGDLNELFRVHLEKAVNTLLKTELTAFLDY
                     EKYDRIGFNTGNSRNGSYDRTVKTEYGELHLQIPRDRNGEFKQQTVPAYRRTNDTLEE
                     TVIHLFRKGITMSEIADLIEKMYGHYYTPQTMSNITKSFTEEVTAFNGRELHDRYAAI
                     YMDATYIPLKRKTVAKEAIHIAVGIRPDGSKEVLSYAIAPTESITIWEEILLDLQERG
                     LKNVLLFITDGLKGMVGAISRFYPKARFQHCCVHVSRNISHKVRVDDRKEVCDDFKMV
                     YQASSKEVALEARGTFAEKWKTSYPKVVESILSNDHLLTFYDFPLAIRKSIYSTNLIE
                     SFNKQIKKYSHRKEQFQNEESMERFLVSSFDTYNQKFLGRSHKGFQQAEGELEQMLSQ
                     LIEN"
     gene            24152..26341
                     /locus_tag="IWM35_001576"
     CDS             24152..26341
                     /locus_tag="IWM35_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000470930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP9919436.1"
                     /translation="MFISTLQIRNFRNYESEIFRFDNETNTIIGENDSGKTNALTALR
                     ILLDDSYYYSSKTLKESDFFHGIQNGWQGHWIIISATFEGISEEEFDNEICASISLNS
                     ESQTILEELISNADKGVGSISLFIRPNKAIRKQLFDISTELDSHQFNEFRNSIRLSDY
                     EFYYTSKSNLNFCINENYDKLVGRLNDNIASNPEEDDEALLGSRIDMSDIFKHISVVY
                     VDALRDVLREMKNNRNPVKRIMETIESKISSDNVESLKTIIQQLNETITSVPEIRKIG
                     ENINRQLNSIIGSVYSPNLLLSSTMSDDMGSLAKFLSMKPEQNIDLDLLGLGHLNMIY
                     LALKIVEFEACRSRELLNIMLIEEPEAHIHHHIQKTLFEGLNLQKNYTQILMTTHSVH
                     LAEVSEISRMNVLKSFNGKSIVMNPANNLNSFAKEKLKKNNLNLINAVERYLDVKRNG
                     LLFSKGVILVEGDAEEILIPQIVKKLFGINLDELGIGLINVGSTSFEYIASLFDDDRI
                     QRKCAIVTDLDEQAIPSEHRLYKSTAQEKGQARKEKLSSLFNENNWVNCFYANTTFEI
                     EFLDVNKENILTYVSPIIDDAFKDKKTITAYKEEIQSEQYLERNLAMLKLANHVGKGW
                     FAIELGEKIDFLIKIPKYILNAIAFACQEIVDLSIYKKILLHTIDLYEETTELSALSA
                     SLKTSDTADLNIAIISLLEYFGKDEDVVYLINQIENYTKILYAEVSK"
     gene            26338..>27566
                     /locus_tag="IWM35_001577"
     CDS             26338..>27566
                     /locus_tag="IWM35_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAP9919437.1"
                     /translation="MTNIFEKIVNDLNLEQKDVVESEDNLYVNACPGSGKTRVLTRKI
                     AYQAIKHDGSLKRIIAITYTNRAAEEIKERLSLLSIDDDINIWVGTIHQFCLEFIIYP
                     FKMNLSRVSKGFTIIDDYTQRAYVSQINNMLNLNLKTYELNKIDLSLSHDFKINEVQY
                     TQVSEMYHTMLEKNKEIDFDMILLVAYKILTVNSNVARNIASNIRAIYVDEFQDTREL
                     QYNVIAKLLQNNPQIQSMFVGDIDQAIYGSLDGIAKSVEELEMLTSHTFQSKTLHGCY
                     RSNQRLVDFYSNFQSCPYEIESRGNNKNDRGFISYDCKVTKEDLPNIIKNIIKEKIES
                     GIHEKDICVLAPQHYPLFSLGEYLKKELPYCNFDAINISPIKVNNHSLFFKLAILYFT
                     ESGEKVRRRKLIANDIL"
BASE COUNT         8838 a         4564 c         5442 g         8722 t
ORIGIN      
        1 ccactaaacg gtgttctcct gccaactcct cggtcacaag tcacaatatt caacaaacat
       61 gagaagctgt tttttgaaat tgttccttgt gactcggagc taaacggcag gctcacaacc
      121 tcttggtaaa cggtgttcaa tcagctgacc ctcggtatta gctcaaaaaa cggcaaaata
      181 tgaggaacta ttttttccgt ttttttctct aatactcagg tcaaaacgct gatttcacaa
      241 cctctgattc acggtgttcc agaagtagct tctacggaaa taagccgaaa tttcacaaaa
      301 atttgagaag caattttcgt aaattccttc ttatttcttg aagctgacca cttctgtcac
      361 aacctcttat tttgattaga acttgcgaaa ataaaccggt taaagtgtgt ctagcgcaag
      421 agatttgcta taatagacga taagggtaaa atgtaacgag aaggggttga aaaatgtgta
      481 tcaaattgta acagattctt gttgtgatct gccataccat gtattagcaa aagaaaaagt
      541 tgatttttta tcaatgaatc tccaattgaa tgatcaagag ttagtagatg acctcggaaa
      601 gacattcaat tatgaccgct acatgaatca gctgaaaaat ggtgcgatgc ctacgacatc
      661 acaaatcaat gttggccgat atttagaatt tttccgttct tatgcggaaa aacagattcc
      721 tgttttatat gtggcttttt ccagtggatt aagcggctct tacagtagtg cgcttcaagc
      781 ggttgaattg ctgaaggaag aatatgaaga ccctgagatc tatgtttttg atacaaaagc
      841 tgcaagtatt ggggaagggc ttttagtatt ggaagctgta agattgaaaa aagaaggtaa
      901 aagcatcgaa gaaaccctag agtggctgga aagtaattat ttgaaagtcc attcatgggt
      961 aacagtagac gatctgaaat atctagaacg aggcggtagg atttccaaag caagtgctac
     1021 tcttggcagt ctattgagtg taaagcctat tattacggtg gataaagaag gaaggctgac
     1081 gaatgttgac aaagttcgag gaagaaacaa gtcgatccag acaatcgtca atgagacggt
     1141 aaaaacaatc gttgcaccac ttgatcaaat cgtttatgtg gcttacgctg gggatctaga
     1201 gagcgcggag aaggcaaaac ggcttttgga agagcagata aagatgaaag atgtgaagct
     1261 ttatccctta ggaccaacga tcgccagtca cacaggctat ggctgtatcg cgattttttc
     1321 gatgggaatc agtcgataaa agcagtaact cattctgtaa aaaagcccag aaattgattg
     1381 taacaaaaac agcgactctt aaaagataga tgagcatcct atctcttaga ggtcgctgtt
     1441 tttttatttt ttaagctaaa taggccatta tgcccataac tcgttcaatt gtttttgaat
     1501 atctgcattt tccaaaaatt catcatatgt cgtatcagct cgatcgatca cacctttatc
     1561 cgaaacggca ataatgcggt ttgccaatgt ttggataaat tgatggtcat gtgacgcaaa
     1621 taagattgag ccagtaaatg ccatcaagcc gtcattcaat gctgtgattg attctaagtc
     1681 taaatggttc gttggatcat ctaatacaag tacgttagct ttcgaaagca tcatttttga
     1741 aagcatgcaa cggactttct ctccaccaga taaaacattc actggtttca atacttcttc
     1801 accagaaaac aacattcttc ctaagaagct tcttaagaag gtgttatcat cttcttcttt
     1861 acttgcatat tgacgcaacc agtctaaaat cgttagatca gtatcgaatt ctttcgttgt
     1921 atcctttggt aaataagaat tacttgttgt cacaccccaa cgtactgttc cagtatctgg
     1981 cgtaatttca cccatgataa ctttgaatag cgttgttgtt gttatatcat tttctgcgac
     2041 gaaagcgacc ttgtcttctt tgctcaaatt aaaagatata tcatctaaaa ttttctttcc
     2101 atcaatcgtt acacttacat tttctacctg caataagtcg tttccaatct cacgttcagg
     2161 tttgaatcca acgaatggat aacgtctaga agaaggttgg atatcgtcta aagtaatttt
     2221 ttcaagcatt tttttacgtg aagttgcttg ttttgatttt gatgcattcg cactaaagcg
     2281 ggcaataaat tcttggagtt ctttgatttg ttcttctttt ttggcatttg cgtttgcttg
     2341 aagtttagcc gctagttgac tagattccaa ccaaaaatca taatttccta cataaagttt
     2401 gattttacca aaatctagat ccgccatatg tgtacatact ttgttcaaaa agtgacggtc
     2461 atgggataca acgatcacag tattttcaaa attgatcaaa aattcttcca gccaattgat
     2521 cgattggata tccagaccgt ttgttggttc gtctagcaaa agaacatcag gtttaccaaa
     2581 tagtgtttgt gctaataaca cttttacctt ttgtccacca gttaattcac tcatttgctg
     2641 atggtgcaat tcttcaggga tgttcagacc ttgcagtaaa acagctgctt ctggttcagc
     2701 ttcccatcca ttaagctcag caaattctcc ttctaattca gctgctcgaa taccatcttc
     2761 gtcagtgaaa tcctctttca tgtaaatcgc atctttttct ttcagcactt catacagacg
     2821 cttgtgtccc atgatgactg tttcaagtac agtatagtct tcatagtcgt aatggttctg
     2881 tttcaatacg gccatccgtt catctggtcc catagtaacg gaacctgtgg aaggctgcag
     2941 atcccctgat aacactttaa ggaacgttga tttacctgca ccatttgcac caatcaagcc
     3001 atagcagttt ccgggggtaa atttaatatt cacatcatca aatagtttgc ggtctgaaaa
     3061 gtgcaaactc acatcattta cagtaatcaa tttttttcct cacttttatc attattagtt
     3121 ttgacaagtc ttatagaatt atatagggga aaggtaaaag attcaaggca atcgaacatt
     3181 ctttgtgttt cacgtgaaac agaatgaaaa aaataaaaat aaaagcggag cgaaaatagc
     3241 tactatagga tttattagtt tgcaacagca taatttttga cgttatttat aagagggagt
     3301 actataaaac tgtaaaagaa atgaaagaag gattgaaaat ggatgaaaaa agaaccatcg
     3361 actggggctc attgattttg ggaattttat ttgtacttgt ctcattgtta tcatttcaag
     3421 accctgtcgg aaatttagtt gcaattgtag tagtctttgc gatttttgcc tttattaaag
     3481 ggatatttga actgtttgta cggaacagat taaaagagct aacaggatat aaaggaaaga
     3541 tgcccttaat aatcgggatc atcgatattc tagttggcgt ctttttctta ttcaatattg
     3601 gtgcaggagt cgcagcattg ccttttgttt ttgcagtatg gtttattgct gattcagttt
     3661 tagcgttatt tactgctgac ttggcaagag gagtaagtga aggatattat tggtttacga
     3721 tcatcgtcaa tattttagga attttattag gaattttctt attgtttaat ccaatttctt
     3781 ctgcgttgac attaagcttt ttagttggat tctattttat gttatttggt atcacacata
     3841 tcgtctatgc atttagataa gtaaaatcaa acaaataaaa aagccggctt ccgattacag
     3901 ttttactgtt gtaattggaa gccggctttt ttatcatgga caaatatgca cgaggtgatt
     3961 tttaggtgtc gagacaaatt tatgcaaaaa tgtgagaagc tatatacaca aattttatct
     4021 taatactcga agcatagcgc agttgtcaca acctcttttc atataattca ttcaatactg
     4081 gcaaatcatg gacttcatat ttttctttga atgcttggat ttcttcttca gaatattttc
     4141 ttggatctaa atgcagatct tctactggca atgggcgctt gttattgatc ttcacatcga
     4201 ttactacagg acgatctgat tttgcagctg catcaaaggc aaatcgaagt tgcgagtatt
     4261 ctgtgactgt aaagctattt gccccgagcg cttctccaac tttaccgaaa tctgcatctt
     4321 ctaaaaatac accaaatttc ttttgttccg tatcttcttg ttctgcttcg atgaaaccaa
     4381 aagaatcatt cgaaaagaca acattaataa ttggaagctg atatttgact tgtgtaatga
     4441 tatcttgcat gaccatagca aacccgccat caccacttaa ggtaaatact tgtttttctg
     4501 ggtagcttag ttgtgcggca attcctcctg gcacaccatt acccatagtt gcgaaccatc
     4561 cagatgtcgt atgtttttgt ttaccgttca tattcaataa acgaatagaa tggatagtcg
     4621 tgttaccgac atctgtgacg aaaatcgcat cgtcttcagc aatacgattg atttctttat
     4681 agacagcctc tggacgaatc ggttcttcct ctcgatcttc aaaagatttc aaccaattta
     4741 cccatttttc tttattttct tgatttgcta ggtaccagct atcagcaggg cgagcatccc
     4801 ctaactcgac aagtctttgc aaagctgtgt ttgcatcacc gaggatcgac aaagatgttt
     4861 catggcgtcg tccaaatttt gacgcatcga tatcgatttg gataaattca gcagttggat
     4921 taaaaaatgc acgtccaaat gggaagtcgc taccgacaaa taagatcaga tcggcttctg
     4981 ccaatgcttc atttccgggt ttagtagcga cacgtccagc aaaaccaaga aagttttcat
     5041 aatcatcagg aataattcct tttgctagaa cggaagcgac aacaggaact gaaaaatgtt
     5101 ctgagaattc tttgatttgc ggaaatccgt ttcgcgttcc ttgaccaaca taaagaactg
     5161 gtttttttgc tttttcaaga taaggcaaag cagctagcaa atctttttct tctggtaata
     5221 tcacacttgt tttgtgagtg tgagcggtgg agaaaggttg ttcgtcaatt tcttcaaaac
     5281 caagatcaac agggatagtc acgatagcca cgcctttatg ctcataggcg gctttaatgg
     5341 cttcatctac tacatgcgga agactttgtg gtgtcatgac tgtacgattg tatacgctaa
     5401 catctgcgaa aataggattt tcatttagtt cctggaaaga gttatagttc atggatgtag
     5461 aagcaacttg tcctaagaac gctactagag gcacatggtc catctgtgca tcatacaaac
     5521 cattgatcaa atgtgtcgca ccaggtcctg cagagccgaa gacagcgccg actttccccg
     5581 ttaattttgc atctgcagcg gctgcgagtg cgccgacttc ttcatgacga acttgaatgt
     5641 agttgatttc ggttttttct tgatatagcg catccataat tgaattgaaa gatcctccgg
     5701 gaatgccgta aatatggtcg ataccccagc tttcaagaac ttttaccatt gcgattcctg
     5761 cattgatttt tgacatggtg tttcactcct ttagcaatga tgatacataa attttttgta
     5821 tctagagtat agaacttttt atcgaaaatc ccaactaatt cgtcttttca caaaaatgta
     5881 gtcatgtttt tgggaatatg ctagagtagt acatagatag attcaagaga ggggaatggc
     5941 atggttgaat ggctattgag acttgaacct tggcagcaag cacttgctgg gacagctttt
     6001 acttatttta tgacagctct tggtgccggg ttggtttttt tctttaaaga aatcaaaaaa
     6061 gaagtgctaa acttgatgtt aggatttgct tctggtgtca tgattgctgc aagtttttgg
     6121 tctttgttag atccggctat tacaaaagca gaagaaaatg gagatattgc ttggctagtt
     6181 gtaagtattg ggtttggttt aggcggtgtg tttttatata tggcagataa aacactgccc
     6241 catatgcatt ttggaccaca acatgaagca gaaggattgc ctacccattt aaaacggacg
     6301 atcttgcttg ttttttctat tacattgcat aacattcctg aaggtttagc cgtgggggta
     6361 gcattcggtg cagcagcaac tgcggataat ccgacagccg ctatattagc cgctgtttcg
     6421 gtggcgctag gtattggtat ccaaaacttc ccagaaggag cagctgtatc aatcccgttg
     6481 cgacaagaag gtctgagtcg taaaaaggcc tttgtttacg gacaagcttc tggaatcgtt
     6541 gaaccaattg ctggagtgat tggagcgcta ctagtaacga aagtggagct gctgcttcct
     6601 tatgcacttg cttttgccgc aggagcaatg atttatgtcg tggtggaaga attgatccct
     6661 gaagcacagc agacattgtc cagcaaacgt cattttgcag tttttggcgt catgagtgga
     6721 tttatcatta tgatgatttt ggatgtggca ttaggataaa taaaaaaaat atatatgata
     6781 tatcaaatag ggccgcgaac ttatatcgcg accctatttt tttagtaagt tatatcataa
     6841 tgaagtgaat ggtatcatac attaataata gttttttcta tgtgtatttc ctttgatcca
     6901 gcgaacaagc cagatgataa gtgcgattgg gattagaact ttgaccgcaa accataaaag
     6961 cccgcttagg acactgacaa aaagactgat gacgaagcta gctgcaatta gcactaaaag
     7021 aaccatgaag atatttaaga acatattttt ttgacgcata caaaaccctc ctcttgttca
     7081 ttctttacta tagaaaaagt ataacaggga ttgtttaaaa acacctccaa ctgaagactg
     7141 atttttactt catctactta aatgatcaaa caattgcttt ttctctggtt tatcatctaa
     7201 aatagagcgg aaaaggagga gaatatcatg cctaaaaatc gtcttgaagc atttacagat
     7261 gctgtaatcg cgattatcat gactatttta gttttagaac ttcatgaacc taacggagac
     7321 acgtgggagg cgctcgctgc attaggttac cgatttttta tctatattgt cagttttatc
     7381 attttagcga tttattggaa caatcatcat catttatttc aagttgtaca taagataaat
     7441 ggacgggtct tatgggcgaa cagtttcttt gtttttgcgc tttcgctttt tccgtttgca
     7501 acttcatgga tcagtgagta catcaatagc ctggctccag aaatgactta tggttttgtt
     7561 accttgaccg ctaatattgc gtattatatc ttggcaaaag aacttgtcaa agcaaataaa
     7621 ggccacgaga aagcattgaa ggcgatgttg aataattaca agaaatcgta tctatcgatt
     7681 ggtttgaatg tgttaggaat tgtcttaggg tatttagtag cgccagtagc cgtgctgatc
     7741 gcaaacatcg tgattctagc tgcttggatc atcccaagtc gtaccatcga atcacaatat
     7801 cggtaaaatg atccgtgttt aaaatttaag agaaaaatat agaaaaaata aataaacgat
     7861 caaatatccg agtgatacta gtattcaaag agggatgcag atggatcaaa aaagaattca
     7921 tgtaggactg attactagtt ttgtcacgat gcttatttat ttggatcaag gtcatttgac
     7981 ttggacagta gttggtgtcg taggattttt gacattattc acagcaataa aaaaataaac
     8041 atctttatgt aatctgatgt tctatctatt taagaaaagc atgtttacag cagtatgatt
     8101 ctgttgtaaa cgtgttttta tttgtggaaa atttaatttt tagaaaagtg tatcgaaaaa
     8161 tagatagtta gtttttccac agtggataac ttcgtaagag ataaaaataa aaaattagtc
     8221 aaagaagggc gtatagattt cttttgatag gagatataca acagaaatga ataaatatta
     8281 acgtgttttc aaacagtata aaacggtgtg gtttttggaa taaaaaaata acatatctca
     8341 ttttacgctt ttgttatttt atacttaaaa aataacgaca cagaaaatac cgtgtctcag
     8401 atttctctac tacttctaaa aactactgtt tttttccttt gaaatgcctt caaaaagtag
     8461 gatcaggcag aatatactgg cagaatcaag gagaagggag cctaaataag gagaaaaacc
     8521 atagaaaata taaagtaatt acttataaaa caagcttttt atagaaaaat aatatacctg
     8581 agtagggtat aaattaagac ggaattcctt tctagattga gatattgact aggaagaatg
     8641 ttaagatttt aagcctttaa gctaaaaaaa aacccttttt gttaaataag gggaatatag
     8701 cctgtcagtt tcaaactgca agtcgtcctg ctgcaataat atagtagatt agagatgaaa
     8761 atagattttt ttaaaccaat gactgaataa aaagaccgtc atagatacac attggaatgt
     8821 gtctattacg gtcttttttt acgaacgatt ttattgaaga cggacagttc gggtcaaaaa
     8881 taaaaaagct acgatcagaa ataagaataa aatgtatccc aaaataatgg ctagacggtc
     8941 taacttattt gtcgatagat gtttgtttag aagttgatag gcccataaac ctatcagtcc
     9001 tcctgttgta ttagttatca aatcagtgat gtccgctacg ccaataccga ataggaattg
     9061 aatcaattct gccaagaagc ttacaaggaa aataatccct aattttttag aaaaagaaag
     9121 tctcttgaaa ttgacattca aaagcacccc aaaaggaaaa aagacaatca agttataagt
     9181 ggtctcacgt aataccgtcg tattttcaaa agaaaaagta gaaaaaggga cgagattgac
     9241 attgctgtaa gaataagtga gaaattgtgg aagattaaat gatagcttga ataaaatcaa
     9301 ccataataga aagaataaat aaatccctaa taaaaatttt gatacatacc gcatagacat
     9361 ctcctccatt taaaaagtat accatcgaaa accgctgaaa aatcataaaa aagtatgaag
     9421 tttaaggacc aacgtagtta tcgtacgaaa attagaagct tttttttgtt gggaatccgg
     9481 tttctttaac aaagctaaga tagcgagttg cttggaaaag ttgctatctt ttttctgtca
     9541 aaacgactcg gaatgttcgt tttttgttct aatttctcca agttgtttac tttttgatac
     9601 taagtttatg aaaaatcaag aaatagttgt tatactaggt aatgctgaca aacgggattg
     9661 gcatttcatc ttggttcagc tttttttcca attactagga aggagggata gattgttcaa
     9721 gacttatttt cgtttcaata aaatacaaga taatcctttt cgtatcatga atctgatgct
     9781 acttatcttt attgtattat ggttaggtta cataaatcat gattttttga tttcaagttt
     9841 ttcatcatta gggatcttta cttttttcta ttatcaaaac ataccgatga aatatttgct
     9901 gaaacgtatg tttctgatcg gttgcggact gttggttgct ttcgttttgg ggatcttatc
     9961 gacttatgtt ttatggcttg aaccatttgc cgtagctttg gttgcttttt ccagcagatt
    10021 cgtgttgcga ttattccata tttctaaacc aggtggcttg ttttttgcga tgctttctgc
    10081 catgggaacg agcatgcagc tgccaatcgc tcaattaccg atcgtctcac tttatttttt
    10141 tatgggtgtc gtttttgccc tgattgcggc agtgattaca aaattactgg attcacggcc
    10201 tgagcagaca attgaaaaag caactttaaa agaaagattt cacgaagagc ctttagtgat
    10261 cattgattct gttttctaca gcgctgcctt atttctttcc gtttatgtca gtcacggctt
    10321 gaatctgcat aatccttatt ggttgacctt gtcatgtgca tcgattttgc tagcagaaaa
    10381 cttggatgcg atgaagcatc gacaagtcca atatctgatt ggatcgatgg gtgggttatg
    10441 tgtgtcggca ttcttgtcat ttgtgccgtt tacgcagttg cagaccattt ttctgatcac
    10501 ttttctatat ggaatcgcac aatttctagt tgctagaaat tatgcagttg ctaatatttt
    10561 tcttaatccg atggcgttga tgctatctac acttattcgt ggagcatact tgatttcttt
    10621 gatcgaatat cgttttttag ggatcgtcat aggaagtttc atcggtttag gtgttgcgtg
    10681 ggtaatgacg gttggtttgc aacattattt aacggttgtt cgtcaaaaat tagaatagaa
    10741 ggacaaatat atgtgccaaa agaggttatg acaagatttt tgtcacaacc tctaattcaa
    10801 ggtgttcaaa agccagattc tcagcattaa gtcaaattca cacaaaaatg tggaaaggaa
    10861 ctttcaaaaa aattttatat ttattctatc aaaaaatttt gtttatgatg gtaactgaat
    10921 aaactttcct gagatggtca atgctttcct tgaaagtctt acagatattc tttcttcata
    10981 gaaagtttaa gttttatagt tagtgtacaa taaaagtatg tttataccat atggtgtccg
    11041 gataatcagt catttaaaca atgaggttca aagatatgaa aaggggaaat ttgatgaaga
    11101 aaataagcaa tgaaatccaa attctggcag aaactttaac tcttcccaaa gtcgttatag
    11161 agcaaatgat tgtacaagca aaaaacatcc ttgatgaggt gtttgagcaa tatagtcatg
    11221 aactaatgga tcctaataaa gcaaaggcga ctgcaaagaa aatagacaaa gtctatgaag
    11281 aggaaccttt tgcagcatta gctatttatc tatatgcagc ttctcaaagt tggaaaaaaa
    11341 tttatcaacc attgaagatt ccaagaacta tctatttggc aacaatgaat gcttttacta
    11401 gatttattca tgaacatttc caagtaacag gaagctatcg ctttgaccgt ggtttttgga
    11461 tttggcatta cactagtggc ttgatctttc ggattggtga attggagtac gaaatgacat
    11521 attttccgaa agggtacacg gatctaagat tagaaggaaa gcagtgcctt tctcttcata
    11581 ttccttccga tgcagattta tctcagaata agataagaga tagttacgaa agtgccattc
    11641 gtttttttga actttatttt cctgattacc attaccaagt gatgtacaca gatacttggt
    11701 tactatcacc caatcttact aaatggttga aaaaagagtc aaaaatctgt ctttttgcag
    11761 ccgattatcg attattatca gttgatgaac aggacgatag tggagtccct tggatctttg
    11821 gacgagtaga tgcccaaata catgattatc cagaaagtac gagtttacag cgacaagcaa
    11881 aagagcaatt gcttgcaggc gagcacattg gctctggctt aggtattctt acttcataag
    11941 tttagtactt cgaaatagtt taaaagagcg tgagacaaaa gtaaagaaca cttttgtctc
    12001 acgctctaaa cacgtataaa cggcggaagc agaagcaact ccttcggaaa taagctgaaa
    12061 ttcacaaaaa tttgaaaagc aattttcgtg aatttcttct tatttcttgg agttaaacgc
    12121 ttctgtccca gcctcttttt tagtgttcta tcgaacacac agtacgttca ataggtaaac
    12181 tagctataac aatagatatt aaagttaacg gacaagaggg ccttttttag gtagctaaaa
    12241 cgtaataatg aaaatttatt tccagagata ggagtcaatc agctagtctg atcgactgtt
    12301 ttaacaagtt gactttcatt ttaacttttg ataccattta aaacacaaat cgtaattgtt
    12361 tcgatttgtg ttttttagca tgactacaat caaagaagaa agtgggtcgt ccatatggaa
    12421 aaaacagata tctcaagtgc ataccgccgc ttgaaaagcc caaatatcaa aacacgaaaa
    12481 cgtgcgctga agatcatcaa agatgtgaaa cggaatagtg ggaaaaggta agaagaagga
    12541 aacttaatta aaaaaatagc cgaaaaacgt ttttcggcta ttttcaataa tcggtttttg
    12601 tgtaaggttt ccgaaagaac tgctgttgat tttccatttt taacatatga taaattggta
    12661 gaatctttct tttcaaattg ataccattcc ctaattccag ttatatagtt aactacagaa
    12721 ccacaaagca cgtacgttta taaaagtatt atctgtagtt atttttcaat tccctttcaa
    12781 cctcttattt tgaccagtag tattgatcac tttacctttt aacacctttg ttcgtttttg
    12841 aaacattttt agtcaagaat ggaaacgtaa ctacataatc aaaggttttg cgaaatattt
    12901 catgaaatat ttcatgtaga agcgagcaat acggttgcaa ataaccatta tcgtttaata
    12961 agcagttacg aaagctccca atatctctaa cgttcagctc actccatttc ttttcttcaa
    13021 aaaaatcagt gattcgtatc tctttctatt tgtcgaaaaa gttaacttta aaaagtaacg
    13081 ccgaaaaaat atgcaaaaaa ataaaaaagc agttactatt gctagaataa catcaaggaa
    13141 taataagttt tggttttcag ataatagtcg ataagttgtg tattggaatt ttagatacag
    13201 taataaagta tttttcatga aattttgtga gaatcactat tacaagtatc tttaataaaa
    13261 ttaatggtta aggaggaact taaagtagaa tgtaaatgaa tagttgtagt cagtcgattg
    13321 tgatactaga ctcaaagttg caggaataag tttttcaatt tgtgattgga agataaaaaa
    13381 tgaaaaagaa gggctttttt ttatactttg ttgattgtag tgtttatttt ccctctgttt
    13441 agccaagtaa cattttctat gactcaacca gtttcaagtg aggaacatga taaccttttt
    13501 cgtgctacag aagagtttaa gaaatatcag gaaaatattg ttccggagac tccattagct
    13561 agtgagccaa ttactgatat tgatggaaac caaataggat ggtatataca gtacgaagtt
    13621 atggtggtaa ataatgactc aatagtaaaa aatacagtag gcttagagct aacaccgatt
    13681 gaaaaggtga gttcattgtt aacgtatatt tatactgatg aaagtgacga gactgagatc
    13741 attttaacgg attactcaaa aattgctact gataaaaagt atatattgtg gatgtagaca
    13801 ctagtgaaat actttatgat atagaaattg atgaaaattt actagatgta gcacaagaaa
    13861 tcgatcatca aaagcaggaa gctattcgat atgctggtag aaaagggaaa agaaaatatt
    13921 tattacgtga atgtagcaaa agttagagaa gatgaaaatg agtggaaaga attcaagagt
    13981 cgctactcaa ttaactcgac tccgacattt acagtttaca gagaaggatc gatagaaaaa
    14041 actgtttttt ggactaaaga aagtggaatg tctttagctg aagtagaaga gtttttggat
    14101 tatgttagta tgcaacagta agggagttct tattatgaaa aattttgaca catattatgt
    14161 tattgttttt tcgatatctt tgctgttaat attactaact ttaatgcagt ataaagagta
    14221 tccagagtat tttattttaa cttttgtata tttagtaagt agctacacac ttctatataa
    14281 attcagaagt tcttctagag tttctaaagt ttgtttctta attagcttat tgattctata
    14341 atatttttga tttatatagg aaattaaaaa tattatagaa tcaacactca gagaacgggt
    14401 tggtttttaa tagatttaat aagtactgat aatgaaattt ctcctggttg ggaaaacgaa
    14461 gttggttacc ctgtagaaga attttttcaa gattactttg cggagtatgc cgataggata
    14521 atgagagatt acgtaacaga taaaactcaa gaagtcatca atcaatccca gtcattaaat
    14581 gaattatctg ataaggcgaa agaagaagca gttgccatta tttcttcagg gattagtgac
    14641 tatattgata tgagtggtga aatcaattat ggcgataaag acaaaattgt acgcatgata
    14701 aaagctttta tcaaaacttt tgatgtgaag gcagatctta agtttaatga tacttaccta
    14761 gtaggaaaat taattaatat ccttgggaca gagaaagata cggaaacgtt tatttcctct
    14821 ttgtctatgt tgtttacaga taagcatttt tcaactcagc ttggtggaga ggaactagtg
    14881 gaaatctttt aaccattacg cccagcatta ataaaagtat atcaaaaaag tactcctgat
    14941 gatttttaag attggtttga gaaataatgt gaaaacacta tttttaatgt taattataga
    15001 tagagatgaa cgcgtcatat ggttaaaaga atgaaaaagt caaagataca ctgctatatt
    15061 tcactaagga tatagcagtg tatctttcat gtcgtttcac tggcagtttt ttataaaagg
    15121 aggtgtgcat ttcagttgct tttctttggt agttagttga atcgtttaag aattaacgga
    15181 tgtggcagac aggtgctctg tccaaaaacc agtaaaatct ttgatggttc catctttatt
    15241 tagctgtgcc tgttcaaata ctgttttgtt tttaccgaat ggatcaattg caataattgt
    15301 tccgatcccc gcacgtttgg cagcaagtaa tcctgagtaa gcatcttcga taacaagaca
    15361 atcagccgga gctaagctca gtttgtctgc cgctttaaga aaaatatccg gttgcggttt
    15421 cccagggaaa gagccgtcgt catagacaat cttgtcccag tcaaaccagc gggctaaatc
    15481 gaaaatatca aaataaaaag caacattctc tttgacagta gccgtggcaa tcgttaatgg
    15541 aatctgttgg tcagtcagct ggtcaagagt cgcttcaagc ccttttgtca actgaagctc
    15601 gttttcattt tgcaagcaaa gcatacgata atagctttct ttttcttctg acaatacggc
    15661 gatctcagag gcggtcaaat gctctgaaat gaaatgtttc aaaatctccg tattcgtccg
    15721 accatgaata ttttttaaaa tatcctcgtc tgataaacca tcttttgtat gatcatgtat
    15781 catatgcagc caagcagctt catgcagatg agagtccaaa aacattgttc cgttgaaatc
    15841 aaatataatc cctttcatag ttacgctcct atagtggtat tttcggttga atcagcaatt
    15901 tgcagatcga catattgtgc atagtgctct tgaacctccc gcgagatctg gcgatccgta
    15961 atgagcagat cgaaatcatg caaattataa aacacataaa aatcatctcg attgaatttt
    16021 ttattatcaa cgagtagata tttcttacgg gaattattta gagcgatctg ctgtgcttct
    16081 ccttcttcgg tactgtaagt agagatcgct tcgttgtgaa cgccatttga actgataaat
    16141 gcttttgtaa actttagtct tcttaaattg tcgttggtga tcggtccgac aaatgcgccg
    16201 gtatttttcc ggaaatcacc gccaatcagt agaatttctg gtccatctgc ttgagcaaga
    16261 atatcaaaaa ctggataatt atttgtaatg atccgaattt tcttgttcag taaatgcgtc
    16321 gccaatagtt cgattgttgt acctggtcct aagaaaatcg tttctccctc gtgaatcaag
    16381 gaggctgctg tggcagcaat ttcttttttt tcttgaatct gcagagtggc tttttcagtg
    16441 tgagaaagtt cttgatctaa agtgtaggaa agactttgag cccctccatg gacacgcagc
    16501 agcttgcccg atttttctaa ttcatctaaa tctctgcgaa cggtcatatc agaaacatct
    16561 aattgattga tgatttcatt aacggtaagg attccttttt tattgaccat ttctacgata
    16621 gtaagcaatc tttctttttt caacattgtc ttcacctcta atttaaggat aatctatcat
    16681 ataaacggac aacagtcaat ctaattcgaa caaaagtaaa catttagggt ggtttttatg
    16741 tttgttacaa tagattttat tgctgttttc tggtttttat gattatttgt gtttgttttt
    16801 gttgacaaat gttttggatg cgtttacaat gcaatcagtt agaaagagcg aaaactgatt
    16861 atagatttga ggagagatgt acatgcttaa attaccagaa gattttattt ttggcggagc
    16921 cacagcagcc tatcaggttg aaggagccac taaggaaggt ggcaaaggcg ctgttgcttg
    16981 ggatgacttt ttagaggaac aagggcgatt cagtcccgat ccggcaagtg atttttatca
    17041 tcaatatgca aaggacattg aattgtgtga acgttttggt gtaaatggtc taagattatc
    17101 cattgcttgg agccgcattt ttcctgatgg agctgggaaa ccaaatccag aaggaatcgc
    17161 tttttatcat cgtgtgttcg aagaatgtaa gaaaagaaac gtaacgccgt ttgtgacgtt
    17221 gcatcatttt gataccccta aacgtttgtt tgaccatgga gattttttga atcgagaaac
    17281 aatagaagca tttgtttcct atgccatttt ttgttttcat gaattcaaag aagtcaaggt
    17341 gtggagtacc tttaatgaaa tctatccagt tgcaaccaat caatatttgt taggtgtttt
    17401 cccaccagga atcaaatatg attttacaaa gatcgtcgcc tgtcttcata atatgatggt
    17461 tgctcatgcc cgtgtagtga actatttcaa agaaaacgaa cttccgggtg aaattggtgt
    17521 agttcactca ttagagacga aatatgcggc aacagacgcg ccggaagata agcatgctgc
    17581 ctttttagac gatgcattgt cgatccgttt cttattagat gctacctatt taggctatta
    17641 ttcaactgaa accttgactg ctcttgatga gatatgtgaa gcaaatcagg ccagctatca
    17701 ttttcctgaa gaagatttcg ttgaattgaa aaaagcatcc acgcgtaatg actacttagg
    17761 gatcaatcac taccaatgcc attttgtcaa agcttacgat ggagaaaatg caatccatca
    17821 taacggtacg ggtgaaaaag ggacctctgt ttataaagtg aagggaatcg gagaacgtat
    17881 ttataaagaa ggaattcctc gaactgattg ggactggctg atttatccag aaggtctata
    17941 tgacttgttg ctgcgaatta aaagcgatta cccacattac aacaaaatct atatcactga
    18001 gaacgggatg gggtataaag atcagtttga agatggcatt attatggatc agccgagaat
    18061 cgactatttg agagtctatt tggaatcctt aagcaaggcg ataacagccg gggtaaatgt
    18121 gaaggggtat tttttgtggt cgcttatgga tcttttctca tggacaaatg ggtataacaa
    18181 aaggtatggc ttgttttatg ttgattttga aacccagaag cgttacccaa aagaatcagc
    18241 ttactggtac aagttggtca gtgaaaccaa aacgattatt taataagtga taatggagga
    18301 agaaaagatg aataaagagg aattacaaat gcttggtttt gagatcgttg catattcagg
    18361 cgacgctcgc agtacattat tgaaattatt gaaagaagta agacaaggaa actttgaaca
    18421 tgttgattcg gcgttgaaag aagccgacga aaatttaacc cttgcacata attcgcaaac
    18481 aaaaatttta gcggaagaag catctgggaa agagatggag atgggattca tttttatcca
    18541 tgggcaggat catttaatga ccactttatt gctgagagat ctgattcaag actttattgt
    18601 actgtacaga cagaatcaag gataggagaa gaacgctatg gatggaatca ttaaacaaat
    18661 cgaaaaaggg aaaccatttt ttgaaaaaat ctcacgtaat agttatttag gagccgttcg
    18721 tgacggattt ttaacagcga tgccagctat tttattttca agcattttca ttttgattgc
    18781 tgcggttcct gagatttttg gttaccaatg gcctgaatct gtctccacat ggctatggaa
    18841 agtctataat tattcgatgg gtgttgtagg gttactcgtt tcagcaacca ctgctcgctg
    18901 cttggctgat tcgatgaatc gtcgcttacc gaaaaacaaa gtaatcaaca ccacctctgt
    18961 gatgctggcc tctgtggtaa gtttcctgtt gttaagcgtc tctccgattg aaggtggatt
    19021 ttcaacggaa tatatgggga caaagggact actggcttcc tttgtcgctg catttttaac
    19081 agttaattta tataagtttt gtgtgctgaa agatattacc attaaaatgc ctaaagaagt
    19141 tcctggaacg atttcgcaaa ccttccgaga tatttttcct ttctcttttt cagtatttgc
    19201 ggccgtttta attgatttag gcattcgaca aatcttccaa atctcatttg cagaagcaat
    19261 cattacggtg ctgcagccgc tatttacagc agctgacggg tacttaggaa tcgctattat
    19321 ctggggagcg atggcgctgt tttggtttgt cggtgttcat ggaccgtcga tcgtggagcc
    19381 ggcaattgcg gcaattatct atgccaatgt ggaaaccaat ctgcaattgt tcaattccgg
    19441 tgaacaagct tctaaagtac tgacggtggg tttagggaat tttgtaggaa ctatgggagg
    19501 aactggggca acattggttg ttccttttat cttcctactc tttgctaagt ctaagcagtt
    19561 gaaggcagtt gggaaagctt cctttattcc agtaagtttc gctgtcaacg aaccattgct
    19621 atttgcggca cccatgattt tgaaccctta tttttttatt ccatttttgt taacacctgt
    19681 tgtaaacgtt tgcctgttca agttttttgt cgatgtattg ggcatgaaca gctttatgta
    19741 cgttttgcct tgggcaactc cagcaccgat cggtttggtt ctaggaacgg gcatgggtat
    19801 cttagctttt gttctagcag cggttctaat cgtcgttgac ttcctgatct atctaccatt
    19861 ttgcaaagca tacgatgctg tcttagtaga gaaagaagca caaaaagcag aggtgatcgc
    19921 aaccgaggaa ggtgcttctg ccgatgtacg tgaggaagta cctgtggcag cgagtcaagt
    19981 tatcagcgat gcaaataaag aaatcaatgt gttggtactt tgtgctggcg cagggacaag
    20041 tgcgatgtta gctaatgctt taaaaaaagg tgcatctgaa tcgaaagcac cgatcagtgc
    20101 aatggcgggt gcttatggtt ctcattacga aatgctgcag gattttgata tggtggtctt
    20161 agcaccgcaa gtcaattctt actatgatga tatcaaagaa gacaccgatc gcttaggaat
    20221 caagctggtt gcgacaaaag gagcagaata tatcggatta acgagagatc ctgaaaaagc
    20281 tgttcagttt attctgaacc aatttcaata aaggaggagc cataatgaaa atgggaattt
    20341 ggttgcgtaa agctgtttta tttgcagctg tgttaggggc tttgggggct gtttcaaaaa
    20401 ctgttgaagc atctgccagc gaatttattc gcggagcaga tatttcgatc ctgtcagata
    20461 tggaaaaatc tggtgcaaaa tattatgaag acgatatttc aaaagatgcc ttagagattt
    20521 taaaaaataa tggtgtcaac tatgttcgac tgcgtttgtg gcaggatcct tataatagta
    20581 atggagaaag ttatggagca gggacaaatg atctggaaac gacgattgac cttgcaaaac
    20641 gagcaaaaaa tctgggactt aaggtgctgc tggattttca ctacagtgat ttttgggttg
    20701 atcctgggaa acagaatctt cctaaagcat ggcaaggtct gacctttgaa gaaatgaata
    20761 cggcattgta cgactataca aagaacgttc tgtcggaaat gaagcagctg gacgtttatc
    20821 ctgatatggt tcagatcggc aatgaactga atagcggaat gctctggcct tatggcaagt
    20881 cttggggaga aggcggggga gaatttgatc gattggctgc ttttttgaag tctggtatcc
    20941 aagctgttcg agacactcaa cctaaaacaa ctccagtaat gctccactta gctgatggtg
    21001 gcgatacagg agcatttact tggtggtttg atgaaatcac gagtcgaggt gtgtcatttg
    21061 atttaattgg agtgtcgtat tatccgtatt ggcacggttc aatgaatgaa cttcaaacca
    21121 atatgaatgc gatcagccaa cggtatggta aagaagtgat cgtagttgaa acagcttatg
    21181 ggcatacaac agcgaatgcc gatactatgc caaatgcttt tggtgaagcc gaagcagctg
    21241 cggggggata tgaaccaaca ccgacaggac aagcagagta tttgctggat cttgccgatc
    21301 ggattcaggc agtaccgaat aatcgcgggg cgggcttctt ctattgggaa cctctttggt
    21361 acaacggaaa tgtgagttgg gcaacacaag caggtatgaa ttatctcagt gttcaatcca
    21421 ctatgggaaa cgaatgggac aaccaagcga tgtttgattt tgctggaaac gccttgccaa
    21481 gcttgcgtgc gtttaaacaa gcaggggcgc aaataaatct agttaaaaat gcaagtttcg
    21541 aaagcgatgg accaacgacc agtccttcca attggcaaac ctggtttcaa caaggaaccg
    21601 ctaagggtac gatcaaaacg gaagcgggca ctttttttgg ggactacaaa ctaacttttt
    21661 ggcaggatca agcatacgag gcatcagctt atcaaaatgt tagtggcata agcaaaggaa
    21721 cttacacatt gagtgcctat gtaatgtctg ggggagagtt tgatactaat cagttatata
    21781 tcaaaaatta tggtggagcg gaactaaata ctgcggttgt taaaacaagt gaatggacaa
    21841 aaattacaat caaagacatt ccaattacaa caggtgtttg tgaaattggg gtttatagca
    21901 aggcttcagg aaatgcttgg acgaacgttg attttgtttc attggtgaaa caatagactg
    21961 cggatcgttt cgattcaaga tcggaggttc ccttcagcca catcgttagc gtagaaggca
    22021 ggaatcgaac ctgcacctcg cctttgccaa aaataaccag aaacgatttc cagtcatttt
    22081 taccaaaacg agtaaagcac cagacttcat ctacactttg tattatgcta aagagagttc
    22141 aacgatttcg taagaagcaa tgaaaaaatt gcaaaaagtt gtgcagataa cataaagttt
    22201 gtttaagaaa gggtaacctt agaattccgt cgtgaatatg atttgagcac ttagatggat
    22261 agaaaaaagt tctcggaaaa cagttcgttt ttcgagaact tctttttatg ataagaatga
    22321 aacaaattaa cttatattat agtctctttt ggtagggaac catcttattt ttaatgaaaa
    22381 tcaagcattt tagctattga agaatgttga tttataggga ttccccctcc atattatgtt
    22441 ggctctttat tcccactctg ttttcaacgt aaagtgtata aaacagaatg cgatactatc
    22501 aagggagtta aaacaatata cagaacagta aaacactata aataaaaata aaagtgcaat
    22561 aaaagtgcaa tttttgcacc taatcatact ctgtttatat atgaaaacta gattctgaat
    22621 ccaatatttg gttaacgaga ggcgttcgaa tttgaacgtc ttttatatat tactttcaaa
    22681 taaatatgag gtagaatact atctattttc tttttaaccc aattgttata atggagagtg
    22741 taaaatattt tgtgtaaata gaaaaaagga agtcccttct gtagaataga gttaccacaa
    22801 cacattcaca gaagagagga cttccctatg aacgatttta ctacagaaat tctaaagact
    22861 ctagcgaaca aaggcgattt gaatgaatta ttccgtgtcc atttggagaa agcagtcaat
    22921 acgcttctca aaacggagtt aacggctttc ctagattacg aaaagtatga tcgcattggt
    22981 tttaacacgg gtaattctcg taacggctcc tatgaccgta cggtcaagac cgagtatggg
    23041 gaacttcatc tccagattcc gcgcgaccgc aatggtgagt tcaagcaaca gactgttcct
    23101 gcttatagac ggacgaatga cacgttagag gagaccgtca ttcacctctt ccgaaaaggt
    23161 attaccatgt cggaaatcgc agacttgatt gagaaaatgt atgggcatta ctacacgccc
    23221 caaaccatgt ccaatataac aaaatcattt acagaagagg taacggcgtt taatgggcgg
    23281 gagcttcatg accgttatgc tgctatttat atggacgcaa cgtatattcc gttaaagcgg
    23341 aaaaccgtcg ccaaggaagc tattcatatc gcagttggca ttcgcccgga cggatcaaag
    23401 gaagtattga gctatgcgat tgcaccgact gaatccatca cgatttggga ggaaatttta
    23461 ttggaccttc aagagcgcgg tttgaaaaat gtcctcctgt tcatcacgga tggcttaaag
    23521 gggatggtag gagcgattag tcggttctat cccaaagctc gttttcaaca ttgttgtgta
    23581 cacgtttccc gtaatatcag tcataaagtg cgtgtcgatg atcgtaagga agtctgtgat
    23641 gattttaaaa tggtgtatca agcctcatct aaagaggtgg cgttggaagc acgtggtact
    23701 tttgcggaaa aatggaaaac cagctatcca aaagtggttg aatcgattct ttcgaacgat
    23761 cacttgctca ctttctatga tttccccttg gccatacgca agagtattta ttctacgaac
    23821 ttgattgaat cctttaataa acaaatcaag aaatacagcc accgcaagga acagttccaa
    23881 aatgaagagt cgatggaacg tttcttagtc tcgtcttttg atacttacaa ccaaaaattc
    23941 ctaggtcgca gtcataaagg tttccaacag gccgaaggcg agctcgaaca aatgctaagc
    24001 caactgattg agaattagaa acaatctgca gtagggaaga atcatttaca caaaattatt
    24061 gacgctccct tataatgaga acttttacta acataattta tgataaactt actatataat
    24121 catattaatt aaatagttag gagatctgct tatgtttata tctactcttc aaattcgaaa
    24181 ttttagaaat tatgaatccg aaatctttcg attcgataat gagacaaata ctattatagg
    24241 tgagaatgat tcagggaaaa ccaatgctct tactgcattg agaatcttat tagatgatag
    24301 ttattactat agtagtaaaa cactaaaaga atcagatttt tttcatggaa ttcaaaatgg
    24361 gtggcaaggg cattggatta ttatctctgc gacatttgaa ggaatcagcg aagaagaatt
    24421 tgacaatgag atttgtgctt ccatttcttt aaattctgaa agtcaaacaa tcttagaaga
    24481 attgatttca aatgcagata aaggtgtagg tagtatttct ctatttatta gacctaacaa
    24541 agctattaga aagcaattat ttgatatttc cactgaatta gactctcatc aatttaacga
    24601 atttagaaat tctattcgtc tatccgacta tgaattttac tacacatcta aatcaaatct
    24661 aaatttctgt atcaatgaaa attatgataa attggtagga agactcaatg acaatatagc
    24721 tagtaatcct gaagaagacg atgaagctct gttgggctca agaattgaca tgtcagatat
    24781 tttcaagcat atttcggttg tttatgttga cgctcttcga gatgttctta gagaaatgaa
    24841 aaacaatcga aacccagtca aaagaatcat ggaaacaatt gaatcaaaaa tatcatcaga
    24901 taatgttgag tcattaaaaa caattataca gcaattaaat gagactatta ccagtgttcc
    24961 tgaaattagg aaaattggcg aaaatattaa tcggcaacta aatagcataa ttggttcagt
    25021 gtattctcct aatcttcttc tttcctcaac tatgtcagat gatatgggtt ctttagcaaa
    25081 atttctttca atgaagccag aacaaaatat cgacttagac ttactagggt taggacatct
    25141 aaatatgatt tatttagctt tgaaaatagt cgaatttgaa gcgtgtcgtt caagagaact
    25201 acttaatatt atgttgattg aagaaccaga agctcatatt catcatcata ttcaaaaaac
    25261 tctgttcgaa ggattaaatc ttcaaaaaaa ctatactcag attctcatga ccacacattc
    25321 tgtacattta gctgaagtgt ctgaaatatc tcggatgaat gtattaaaat cgtttaatgg
    25381 caaatctatc gttatgaatc cagcaaataa tctgaactca tttgctaaag aaaagttaaa
    25441 aaagaacaat ctaaatctaa taaacgcagt agagagatat ttagatgtta aaagaaatgg
    25501 attgcttttt tctaaaggtg ttatcttagt tgaaggagat gctgaagaaa tacttattcc
    25561 tcaaatagta aaaaaattat ttggtataaa cttagacgaa cttggaatag gtttgattaa
    25621 tgttggtagt acttcgtttg aatatatcgc tagcttgttt gacgatgata gaatacagag
    25681 aaagtgtgct attgtaactg atttagatga acaggcaatc ccatcagaac atcgattgta
    25741 taaaagcact gcacaagaga aaggtcaagc aagaaaagaa aaattatcct ccctttttaa
    25801 tgaaaacaat tgggttaatt gtttttatgc taatacaaca tttgaaattg agtttttaga
    25861 tgtgaataaa gaaaatattc taacttatgt cagtccaatt atagatgacg cattcaaaga
    25921 taaaaaaaca ataactgctt ataaagaaga aatacaaagt gaacaatatt tagaaagaaa
    25981 tttagcgatg ttaaaactag ccaatcatgt tggtaagggg tggtttgcaa ttgagttagg
    26041 tgaaaaaatt gactttttaa tcaaaattcc taagtatatt cttaatgcta ttgcttttgc
    26101 atgtcaggaa atagtagatc tctcaattta taaaaaaatt ctactccata caatcgactt
    26161 gtacgaggaa actacagaat tgtcagcttt atcagctagt ttaaaaacaa gtgatacagc
    26221 tgatttgaat atagcaataa tttctttatt agagtacttt ggtaaagatg aggatgttgt
    26281 gtatctaatc aatcagatag aaaattacac aaaaatactt tatgctgagg tttcaaaatg
    26341 acaaatatct tcgaaaaaat cgtaaatgat ttaaatttag aacaaaaaga tgttgtggaa
    26401 agtgaagata acctttatgt aaatgcatgt cctgggagtg ggaaaacaag ggtacttaca
    26461 aggaaaattg cttaccaagc aattaaacac gatggatcac taaaaagaat aattgctatc
    26521 acatacacta atagagctgc tgaagaaatt aaggaacgcc taagtttact tagtatagat
    26581 gatgatatta atatttgggt ggggacaata catcagtttt gtcttgaatt tattatttat
    26641 ccttttaaaa tgaatctctc aagagtatca aaaggattta caattattga tgattatact
    26701 caaagagctt acgtcagcca aattaataat atgctgaatt taaatctcaa aacgtatgaa
    26761 ctgaacaaaa ttgatttgtc actttctcat gattttaaaa ttaatgaagt tcagtatact
    26821 caagtttctg aaatgtatca cactatgttg gaaaaaaata aagaaattga ttttgatatg
    26881 attttactag tagcgtataa aattttaact gtaaacagta acgttgcacg taatattgct
    26941 agtaatataa gggcgattta tgtggatgaa tttcaagata ctagagaatt acaatacaat
    27001 gtaatagcta aattacttca aaacaaccct caaattcagt caatgtttgt tggagatatt
    27061 gaccaagcaa tttatggatc attagatggt atagctaaat cagttgaaga actagaaatg
    27121 ctaactagtc acacttttca atcaaagacg ctacatggct gttacaggtc taatcagaga
    27181 ttagtagact tctactctaa ttttcagagt tgtccttacg agattgaaag cagagggaat
    27241 aataaaaacg accgtggttt tataagttat gattgcaaag ttactaagga ggatttaccc
    27301 aacataataa aaaatataat aaaggaaaag attgaaagcg gcattcatga aaaagatatt
    27361 tgtgtacttg ctcctcaaca ctatcctctg ttttcactgg gtgagtattt aaagaaagaa
    27421 ttaccgtatt gcaattttga tgctataaat atatcaccaa ttaaggtaaa taatcatagt
    27481 ttatttttca agttagcaat tttgtacttt actgaatcag gtgaaaaggt tcgaagaaga
    27541 aagctaattg caaacgatat tttaat
//
LOCUS       DACDXO010000032        26615 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E23605_2017
            SAMN12639989-rid9881463.denovo.032, whole genome shotgun sequence.
ACCESSION   DACDXO010000032 DACDXO010000000
VERSION     DACDXO010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639989
            Sequence Read Archive: SRR10023389
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741914.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:42:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,972
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 33 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 17 of 73
            Pseudo Genes (multiple problems)  :: 10 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26615
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639989-rid9881463.denovo.032"
                     /isolate="E23605_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            257..1450
                     /locus_tag="IWM35_001578"
     CDS             257..1450
                     /locus_tag="IWM35_001578"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HAP9919438.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     gene            1580..3052
                     /locus_tag="IWM35_001579"
     CDS             1580..3052
                     /locus_tag="IWM35_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP9919439.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            3135..3923
                     /locus_tag="IWM35_001580"
     CDS             3135..3923
                     /locus_tag="IWM35_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HAP9919440.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(3990..4658)
                     /locus_tag="IWM35_001581"
     CDS             complement(3990..4658)
                     /locus_tag="IWM35_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HAP9919441.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQIMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            4773..6485
                     /locus_tag="IWM35_001582"
     CDS             4773..6485
                     /locus_tag="IWM35_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9919442.1"
                     /translation="MKLILRHAKKYKVAVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            6488..8257
                     /locus_tag="IWM35_001583"
     CDS             6488..8257
                     /locus_tag="IWM35_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010762530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9919443.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENCKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFE"
     gene            complement(8462..9028)
                     /locus_tag="IWM35_001584"
     CDS             complement(8462..9028)
                     /locus_tag="IWM35_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAP9919444.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(9028..9984)
                     /locus_tag="IWM35_001585"
     CDS             complement(9028..9984)
                     /locus_tag="IWM35_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HAP9919445.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(9984..10640)
                     /locus_tag="IWM35_001586"
     CDS             complement(9984..10640)
                     /locus_tag="IWM35_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HAP9919446.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
     gene            11010..13028
                     /locus_tag="IWM35_001587"
     CDS             11010..13028
                     /locus_tag="IWM35_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP9919447.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     gene            13455..15869
                     /locus_tag="IWM35_001588"
     CDS             13455..15869
                     /locus_tag="IWM35_001588"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016183651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HAP9919448.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            16217..17770
                     /locus_tag="IWM35_001589"
     CDS             16217..17770
                     /locus_tag="IWM35_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HAP9919449.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(18151..19935)
                     /locus_tag="IWM35_001590"
     CDS             complement(18151..19935)
                     /locus_tag="IWM35_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9919450.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNCAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(19950..21671)
                     /locus_tag="IWM35_001591"
     CDS             complement(19950..21671)
                     /locus_tag="IWM35_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9919451.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLEIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            21771..22532
                     /locus_tag="IWM35_001592"
     CDS             21771..22532
                     /locus_tag="IWM35_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP9919452.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
     gene            23499..23767
                     /locus_tag="IWM35_001593"
                     /pseudo
     CDS             23499..23767
                     /locus_tag="IWM35_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
     gene            24439..>26615
                     /locus_tag="IWM35_001594"
     CDS             24439..>26615
                     /locus_tag="IWM35_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9919453.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVL"
BASE COUNT         8553 a         4659 c         5522 g         7881 t
ORIGIN      
        1 ctttttgagt atctttttct ttttatctga aagaatgatt ttaaaaagct tagcagagtt
       61 atctttatgg atgattgtgc taagcttttt tggacagttc taatgtaaaa tatttttata
      121 ttttttaagc aaaaaaatga aaaaatacga gcagaaaact gttttatctg ctatacttgg
      181 ttcgttgcaa gttcccgaaa ggatccgcag gacggagatg ccttgtaacc gaatttaact
      241 agggggaatc attataatgg tagaaagaca cttatttaca tcagaatctg tttctgaagg
      301 acatccagat aaaattgctg accaaatcag tgatgcaatc ttggatgcaa ttttaaaaca
      361 agatccaaca gcacgagtag cgtgtgaaac gtctgtaaca actggtcttg ttttagtatt
      421 tggagaaata tctacgacgg catatgtaga tattcaaaaa gtcgtacggg aaacaataaa
      481 agaaattggt tatacacgtg caaaattcgg atttgatgga gatacagcag ctgtattggt
      541 tgcgattgat gaacaatctc ctgatattgc tcaaggagtt gatgaagcac ttgagatccg
      601 ggatgaagat aaaaaagacg tattagatga gattggtgct ggtgaccaag gtttaatgtt
      661 tgggtttgct gtagatgaaa caccagaatt gatgccttta ccaatcgctt tgagtcatcg
      721 tctggtacga cgcttggcag atttgcgtaa atcaaatgaa ttaacgtatt tacgtccaga
      781 tgcaaaatct caagtaacgg ttgaatatga tgatcaagga caaccggaac gcgtagatac
      841 aatcgttatt tcaacacagc atgatgatgc agtagataat gaaacaattc gtcatgatgt
      901 catcgaaaaa gtagtgaagg aagttattcc agctgaatta ttagatgatc aaacgaaata
      961 ttatatcaat ccgactggcc gatttgtcat tggtggtcct caaggggatg ccggattaac
     1021 aggaagaaaa atcattgttg atacgtatgg cggttatgct cgtcatggtg gcggtgcttt
     1081 ttctggtaaa gatgcgacaa aagttgaccg ttctgccagc tatgctgcac gttatattgc
     1141 taaaaacatt gttgcagcag ggcttgctcg caaagcagaa gtacagctag cttatgcgat
     1201 tggtgttgct caacctgttt cgatctcaat caatactttc ggtactggaa cggttccgga
     1261 agaagaactg attgctgcag tgagagaaaa cttcgatctt cggccagcag gaatcatcga
     1321 gatgcttgat ttgcgtcgtc cgatttacaa acaaacagct gcttacggtc attttggtcg
     1381 tacagatgta gatttgcctt gggaacatac ggacaaagta gatgcgttga aagcaagttt
     1441 agcaaaataa gttatattca gctggaacga tcgccaagtg gtggttgttc cagctttttt
     1501 tatggcagtt aacctcttga aaaagatagt aattatttat ctatttttag atcctgtcat
     1561 aatgaaaggg aggaataaga tgaaaaaaga aacaaatgtt actttggtca cagtgagtat
     1621 tttcattgca acgttcatga cggctattga aggtacaatc gtaacaaccg ctatgccaac
     1681 gattgtcggt tcattacatg gaatggaaat catgaactgg gtattttcga tttatttgct
     1741 gacaaacgct atgcttactc cgatttacgg aaaacttgcg gataaagtgg gacggaaacc
     1801 aatctttatt tttgggacat tattgtttat tatcggttcg ttattttgcg gtttatctca
     1861 aagcatgatt gggttgattg ctgctcgtgc gattcaaggg atgggtgctg gggcaatgat
     1921 gccagtagct ttgacgatca tcggcgatct ttattctatt gaaaagcgag caaaagtttt
     1981 aggtttgaat agttcagcct gggggattgc cagtgttttt ggaccgcttg cgggaggatt
     2041 catcgttgaa acgatcagct ggcactggat cttttttatc aatgtgccga tcggaattct
     2101 attaattatt ttgatttggc tatatttaaa tgaaccaaaa atcatacatg agtctaaacc
     2161 aatggatatt tttggtagtt ttagtttgat ggccatgctt gtctcattgc tgttagggtt
     2221 ccaattatta ggagacgaag gaatgagtcc gcttgtttct gctttgctgc taggtagtat
     2281 cgtaatgtta ggtatttttg tttttgccga aaaaagagct aaagatcctg tcatcatgat
     2341 gcatttattc aaaaatccac tatttgttgt ggtcaacttg gctgcagcat tggctagtgg
     2401 ttttttgatg ggaatcgatg tttatatccc tatgtggatg caaggtgtat tagggctgaa
     2461 tgcagcaatt ggtgggctgg tattagcacc attatcacta gtctggatgg tcggttcttt
     2521 ccttgcaagc aagtggatga ctcaaatgaa aactcattgg gtcttgagaa ttggactact
     2581 aatcgcagct attgggggcg cttggttgta tcttatgcct ttcagaagta actatttatg
     2641 gttttttgct atatcaacgg tcatcggtat aggcttaggt gtgaccatga ctacctcgac
     2701 tgtcagtgca caaaatagtg tatctcgtga ccagcttggt gtagcaactt cttttaatac
     2761 gttagttcga acaattggtc aaacagtcat ggtttctatt tttggtttat tgataaatgg
     2821 ggtaactaaa cgagaacttg cagcagcaaa tttaacaaat gatgcagata tcatgaataa
     2881 actggtgaat ccacaaacgg ctaaatatat tgatgcggat attttgttac cattaagaaa
     2941 aattctttat gatgggttac attccgttta tctagttggc ttgttactcg tgatcattgc
     3001 tttggtctta acgcttttcg ttcatgaaaa aagtggtaaa ataaaacagt agtttcggtt
     3061 aagatatgta cgtagccgaa tcattcaaat ttaaagttat tcaaaaataa cttcttacta
     3121 aggaaggcga aaatatggca tttattcaag caaatattta ttcgaatgtg ttagaaatgg
     3181 aagtcaatct aaatgtgata ctaccgcaaa caacaaaaaa ggtgattggc accagttcag
     3241 agcaagagac aacggatata cctgttttat atttacttca tggaatgggt ggcaatcata
     3301 gtgtatggga aagacgtacg tcgattgaac gttatgtagc aaactacgga ttagctgtca
     3361 tcatgccatc tactgatctt ggctggtaca cagatacagt ttatgacatg aagtattgga
     3421 cattcattgc agaagagcta cctgagattt gtcatgaatt attcccgcaa ttaacaagaa
     3481 aaagagaaaa aacatatgcc gctggattat cgatgggcgg atatggcgcg ttaaaactag
     3541 gccttgcaaa atcagaaaat ttcgcagcag taatctctct ttcaggcgct gtttctgtag
     3601 gaaatcgaat ggatgatctt ttgatggttc gtaaagcatc tttttgggaa gggatctttg
     3661 gaccattgga tcaaatcgaa ggttcaaaaa atgatccgca atacttattg aaagaactag
     3721 taaaaagcgg aaaaccagtt ccaagatttt atatggcttg tggggaatct gattttcttt
     3781 atcaggcaaa tcaggaaatg gtatctgcta tgcaggaaca aggcttggcg gtcacctttg
     3841 aacaaggacc tggcgatcat aactggatct tctgggacca atggattcaa cgtgcattgg
     3901 cgtggctaat ggaagaaaaa taaaaaaaga gtcttaaaaa gatagctcct atagagatca
     3961 caaacatagt gatctctata ggagcttttt tactgctcgt tttctcttct tttattttgt
     4021 tggcataagc cttcaatata ggtttgtgca ctcgttagaa agagtgcttt ttcattcaat
     4081 tctgcatggg actgcatact ttctttaaat ccctctaatt gattttcaac tacataatta
     4141 cgcataaata gaactgtttc tttcaaaaat tcatttccgt ccataatttt ttgtcgaagc
     4201 tgctgttcat gctgctgatc cattaactgt ccaatcaata aattgtggag agaggcaatg
     4261 gcctgatgac tttgggtgac tgaaaatcct tcacgaacaa gtaattggat catttgctcc
     4321 aaatgttcta atcgactcgg aaaagcgggc acagtattga tttctaattc tgccccacaa
     4381 gggaaacgtg tataaaggtc atagtaattt tccatatact gcagcaactg ttcattccaa
     4441 ggcaaagttg gatctggaag cgctaattct tcttccattg tttcagccat tatttgcaag
     4501 agctgttgtt tgtttttgaa ataccaatat aaagcaggtg cttgaacatg aattctgttt
     4561 gcgatgtttc gcatggaaag ctgcgaaaga gcttgtgttt cagccaggat atcaaaggcg
     4621 gcctgaatga tgattgcttg agaaagcttt atttccattt tatcaacctc gtgtattgca
     4681 ttatcttttt aaacatggta aattgttagt cggtctaatt ttacagtgtt aaattagtcc
     4741 tgtcaattta aacaaagggg tgtaatcaac gaatgaaatt aatcttacga catgcgaaaa
     4801 aatataaagt agctgttttt atttccttgc tgtccgtagc agtcatggtt gcagcagcat
     4861 tatggcaacc taagttacta cagcaagtat tggaagctat tattacagaa gataatgatg
     4921 aaatgaaaac aattggtatt tacctgatca gtctggcttt gctaggatta gccgcaggtg
     4981 taacgaacac gattttctct gcaaaagtag ctcaaggcgt aagtgcagat atccgagaag
     5041 aagcatttcg aaagatccag acattttcat ttggaaatat cgaacagttc tctgctggaa
     5101 atcttgttgt tcgtctaaca aatgatatca cgcaaatcca aaatttagtg atgatttctt
     5161 tgcaatcact ttttcgaatt ccattcttat ttattggtgc cttcatttta gctatgatca
     5221 cgatgccgca attatggtgg atcatcgtag cgcttatcgt tacggtattt ctgattacgg
     5281 cactttcatt tacaaggatg ggcaagcact tcatgatcat tcaaaaactg attgatcgtg
     5341 tgaatagcat tgcaaaagaa aacttactag ggattcgtgt agtgaaatct tttgtacaag
     5401 aagaaaacca attgaagtca ttcagcaaag taagtgagga cttaacaaaa cacaacatca
     5461 tagtcggtac attattttct gtgatgatcc cgtccttcat gctggctgcc aatttggctg
     5521 tggttggggc gatctttttt gtgagcgatc ttgttaaaga tgatcctacg ttaattggtg
     5581 gaattgcttc attcatgaac tacttgatgc agattatgat ggcaatcatt attggcggaa
     5641 tgatgatgat gatgacttca agagcagccg tatcgctgaa gcggattgcg gaaattttag
     5701 atgctgaacc agatcttact tatttagacg ttccagaaca agagttgacg ggttctgtca
     5761 cttttgacca tgtttctttt cgttatcctg gtgaggatac agatacgctt aaagatatca
     5821 gcttttctat caaacctgga gaaatggttg gtatcgtcgg ggcgactggt gccgggaagt
     5881 caacactagc acaattgatc cctcgtctat ttgatccgac agaaggcaaa gttgaagtag
     5941 gtggtgtaga tcttcgtcaa gtaaatgaaa agagtttaag acaaacggtt tcctttgttt
     6001 tgcaaaaagc aattttattc tcaggaacga ttgctcaaaa cttgcgccaa gggaaaaaga
     6061 atgcaacaga aaaagagatg gaacatgctt cttctattgc tcaagcaaaa gaattcatcg
     6121 aaaagttatc tgatcgttac gaagcaccaa tagaagaacg tagcagtaac ttttctggtg
     6181 gtcaaaagca acgtttatcc attactcgtg gcgttatcgg taatccaaag atcttgatcc
     6241 tagatgacag tacgagtgca ctggatgctc gctctgaaaa gctggtccga gaagcactcg
     6301 atcgagattt gaagggaaca acgacgattg tcatcgcaca aaaaattgct tctgttgtca
     6361 aagcagaccg catcttagta ttggatgaag gcagattagt cggcgaagga acccatgagg
     6421 aattggtagc tggaaaccgt gtctatcagg aaatctttga aactcaaaaa ggaacggagg
     6481 agtaagtatg actgatctaa taaaagcaag taaatttttc tatcactatt tgaagcggta
     6541 caaactctcc tttttcttta tctttatcac agtgatcatt gctacctatc tacaagtaaa
     6601 ggcacctcaa tacatcgggg aagcttttca agaattagct aattacgtag gaggcttgat
     6661 gcaaggcgta gacgataaaa gcaaatttgt ttctgtaata tggaaactct tgctgtttta
     6721 tgtactagct agtgcagcca actttatcta tagtattttg ttcacacaag tagtagggaa
     6781 gtcaacgaac cgaatgcgta tcggtctatt caacaaattg gaaaaattga cgatccgttt
     6841 ctttgattct catcaagacg gtgaaatttt aagtcgtttt accagtgact tagataatat
     6901 tcaaaatagt ttgaaccaag ctttgcttca agtcatcacg aatgctgttt tacttgtcgg
     6961 gatcttgatc atgatgttcc gccaaaatgt agagttagct tgggctacaa ttgcttccac
     7021 tccagtagcc gttttgatag ctgtcttgat tatccgcaaa gcacggaaat atgtggatct
     7081 tcagcaagat gaagtgggga aactaaatgg ttatatggat gaaaaaatca gcggacaacg
     7141 tgtcatcatc acaaatggct tacaagaaga aacgattgaa ggattcttag agcataatca
     7201 aagtgttcgc gaagcaacct tcaaaggaca agcttattct ggtctactat ttccgatgat
     7261 gcaaggaatg tcactggtca atacagcgat agttatcttt ttcggcggct ggttagcatt
     7321 aaatggtgat ttggaacgtt cggtggcatt aggcttagtt gtgacattcg tccagtattc
     7381 acaacaatat taccaaccac tgatgcagat ttcatctgga tacagcatga tccaattagc
     7441 gattacaggg gcaagacgat tgaatgaaat atttgatgaa aaagatgaag tcaaaccaga
     7501 aaactgtaaa gcatttgatg gtgttgaaaa aggtcttgcg ttgcagcacg tagattttgg
     7561 ttattctcca gaaaaactag tcttgaaaga tgtctcgatc aatgtggaca aaggagaaat
     7621 ggttgctctt gttggtccaa caggatcggg gaaaacgacg atcatgaatc tgctaaaccg
     7681 tttttacgat gtcaatgatg gttcagtagt catcgatgga atagatatcc gagattatga
     7741 tcttgatgca ttacgtagac atgtaggaat cgttttgcaa gaatctgtat tgttctccgg
     7801 aacgatcaaa gacaatatca cattcggcaa accggacgct tcagatgaag aagtcatcaa
     7861 tgcagcaaaa caagcgaata ttcatgactt catcatgacg ttagaaaaag gttatgatac
     7921 agagatatcc gaagaaaata acttattcag tacaggtcaa aagcagttga tcagtattgc
     7981 tcgaacgatc atcacaaatc catctttatt gattttggat gaagcaacaa gtaatgtaga
     8041 cacggtaaca gaagcaagga tccaaaaagc aatggatgaa gcaatcaaag gaagaaccag
     8101 ctttgtgatt gctcaccggc tgaaaacaat cttgaatgca gatagaatcg ttgtcttgcg
     8161 tgatggggaa gtcattgaag aaggcagtca tcacgagtta ttagcagaaa atggatttta
     8221 tgcggaactt tatcataatc aatttgtatt tgaataaaga ggttgtgaaa aagctgtcct
     8281 gcatcaagta ataagaacag ccaatcaaaa acagcttctc atgtttttga tttggttgga
     8341 cttattaccg cagatataag cttttgagca ccgtgaataa agaggttatg aaatcaacgc
     8401 tctaacctga gtattagaga aaaaaacgca aaacatatca tttatgtttt gcgtttttta
     8461 tttatctttt tttctcaata caatataaaa taggcggctg gttcttttgg ttgataaacc
     8521 gataattcag cacattatat gtttcctgtg gcagtttgga acaaaaagaa tcaaccatat
     8581 ccagttcttt ttctccacct tcatgaccat agtagacgac aaggatcaat cgtccgcgag
     8641 gcacaagtcg tttcaatatt tcttccatag ctgtgcgtgt agtctctggc atagtgatga
     8701 ttgctttatc actttttggg agatacccta gattgaaaat ccctgctttg agattttgtt
     8761 gttcagctat cacttctcct aacgtttcgt ggccttgtag aaacaatgag acacgttctt
     8821 ctaactgacg ctgttctagc ctttcacgag tagcatcgat tgcttgttgc tggatatcaa
     8881 aactatagac atgaccagtt tttccgatgt gttctgctag aaaaaccgta tcgtggccat
     8941 tgcccatcgt tgcatctacg acgtgatccc ctggttgcaa gatttcttgc agtaatgtat
     9001 ggctgaaatg caaagcagtt tgcaacatca gacgctcacc tttttccgta catcatattt
     9061 tccttggaag ctatctctgc gaagaagttc ttcatcaatt gcattcaaga cttcccattt
     9121 tttcaagctc cacattggtc caatcaagga atcccacggc gcatcccctg tcaaacgatg
     9181 gatgatgatc tcttttggta tcatttccag ctggtcgcaa atgacattga catagtcagg
     9241 acggctcatt aattgaaggc gaccttcatg atagtcccgc aacattctcg ttttgcgcat
     9301 cagatgcatc aaatggagct tgattccttg gatatctgaa tctaaaatgg tgcgtctgac
     9361 attttctttc atcatttcta aactttcgcc tggtaatccg ttgatcaaat gcgtacaaac
     9421 acggatatta tgtttgcgta atttagccac gccatctaaa taagtttgat aatcatgtgc
     9481 tcggttgatc agttttgaag tttcttcaaa agttgtttgc aatccaagtt ctacccataa
     9541 atacatccgc tcgttcagtt cagcaaggta atcaactact tcatctggca gacaatccgg
     9601 tcgtgtaccg actgataatc ctacgacacc tggtaaattg actacttgct caaatcgttc
     9661 tttgattatt tcaactggcg catgggtgtt tgtaaaattt tggaaataaa cgatgtattg
     9721 ttggacatga ggccattttt gatgcatcat gtcgatttct ttctggaatt ggattggaag
     9781 agggtcttca ggtgcaacga tcatgtctcc cgaaccagat acactgcaaa aagtacaacc
     9841 gccatgggca acagtcccat ctcgattcgg acagtcaaat cctccatcga tcggcacctt
     9901 gaaaattttc ccgccaaact catttcgtaa agcgtaattc caagaatgat atcttttatt
     9961 tccttctgaa taaggaaaag tcattaaaag tccttctttc tgtaagagtt atctatttgt
    10021 tttctttttt ctctatacac tggataagtc aggcacagcc aagaaagact gagtaagtaa
    10081 ccgccgatga tatcagtagg aaaatgaacg cccagataaa tgcggcttat cccgacactt
    10141 actataataa acatcatcag caattgaagc aatgtgcgta aggttctttt ttgaacaaaa
    10201 gctggaagca gaaatagtaa agaaccgtaa agaatcatac tgcctgctgc atgtccacta
    10261 gggaaactgt aacttgtttc aactactaag tgcgccaatg aaggacgctc tcgcatgaaa
    10321 aatagtttga tcaccgggtt gatgattcca gcaattacaa taaatccaga aacgaaccaa
    10381 aatgtttctg cccttttttt cttataccac atccataaag ccgcacaagc aaataaaatg
    10441 atgacggtaa gaggattacc gaatttagtc ataaagagtt ggtagctgtt cagatcagga
    10501 taagaagctc tgacaatgcc tgtaaaccac tgatcgaaac cagaaagcca cgacagatgg
    10561 aactttacga tccagcccaa aatgaaaaac aggacaagaa agaagctacc agccacctga
    10621 aatgatgttt tatatttcat acaattcctc ctttgaagca atgacttatt atagcatttt
    10681 tggtccagcg aaaaaaacgg taaagagata aaaccaatgt atgattcctc tttgaataac
    10741 caatattctt ataaaaaaaa taactgtaat cggaaagaca ttttttggta atgcaaatga
    10801 aatgttaggg aaataaaatc agtgtttttc gaaatccttt taaacaagaa cgtagacgta
    10861 ggaagaactt gaaaatgaac agaagttgtt cattacggta aattacaata tgattacaaa
    10921 aaacaacaaa gctcttaaat aacttttaat aaatattaag agaagttata attattaata
    10981 atagataaat tgaagttgga ggatagaaga tggaaaacat tgcaagaaaa gaacggagac
    11041 gtttagaaca gaccaaacgt tttcgaaaag caaaacgagg ggcagcaatc gtgggaactg
    11101 cgatggtagg ctgttctgtc gctgccccat tagtacagcc ggttcctgtt caagcggatg
    11161 aaacacctgt tcagttcggc gcaagaatca atacagcggc atttatcaca gaaattgcta
    11221 cttatgctca accaatcgct caagccaacg atctatatgc atcagtgatg atcgcacaag
    11281 cagttgtaga aagcggttgg ggcagtagtg cgttatctca agcaccttat tataatcttt
    11341 ttgggataaa aggcagctat caaggacaaa ccgtctacat ggatacgctt gaatttttaa
    11401 ataatcaatg ggtaaataaa aaggagcctt tccgacaata tccttctttt gctgaatctt
    11461 tcaatgacaa tgcctatgta cttagaaata cttcatttgg caacggttat tactatgccg
    11521 gtacttggaa gagcaacacg aaatcctata cagatgcgac agcttgtttg acagggcgtt
    11581 atgcgactga tcctggttac gcaggcaaat taaataatat catcgcaact tacggattga
    11641 cccaatatga tacaccagct agcggaaacg caggcggtgg agcgactaca ggtggcaata
    11701 atactggaag tgcaggtagt actggcggtt caagcaattc tggtaataca aactcaggtt
    11761 cagcaacttc gaccacttat actgtccaat ctggtgattc tgtttgggga atctcaaata
    11821 aattcggtat cacaatggat caattgatcc aatggaacaa catccaaaac aatttcattt
    11881 atccaggaca aaaactgaca atcaaaggta gccaggaaaa tggctcttcg acgaataatt
    11941 ccggtaacaa taccaattct tcaggaaatg ctggtacatc aaacggagga caaacaactg
    12001 gtgcgaaata cactgttcaa tctggtgatt ctgtttggaa gatctcaaat gatcacggaa
    12061 ttacgatggc tcaattgatt gaatggaaca atatcaagaa caatttcgtt tatccaggac
    12121 aacagctaat cgtatcaaat ggcggttctt ctaatgctgg agcaaatact ggctcaaata
    12181 ctgggaatac aagtggctct agttcgtcca gctcaagttc ttctaataca gcttcaggaa
    12241 cgaaatatac agttaaagcc ggagactctg tttggagcgt ctcaaataaa tacggaatta
    12301 cgatggctca gctaatccaa tggaacaata tccaaaataa ctttatctac ccaggacaac
    12361 aactgactgt ttcaaacagt ggatcaacaa acagcacgtc aggtaataca ggttcgtcaa
    12421 ccaatacaaa taacagcagt tcagttaata ctggcagcag ttcgtctcaa ggcacttata
    12481 ccgtgaaagc aggtgaatct gtttggagcg tggcaaataa aaacggcatc acgatggatc
    12541 agttaatcga atggaataat atcaaaaaca acttcattta tccaggacag caattgatcg
    12601 ttaaaggtgg aacttcggca aatacaaata caggttcaac tacttctgca gcgaaaccaa
    12661 atacaccgaa tacgtctgct acgacaagta catcatccac tggtaatacg atgtataccg
    12721 tgaaagcggg cgagtcagtt tggagcgtag cgaacaaaca tcatatcacg atggatcagt
    12781 taatcgaatg gaataatatc aaaaataatt tcatctatcc aggacagcaa gtgattgtga
    12841 agaaaggttc atctcaatca acaaaccaac aatcagttac tggttcgaaa acctatactg
    12901 tgaaatctgg agaatctgtt tggagtgttg ctgacagtca tggaattaca atggctcaac
    12961 taatcgaatg gaacaatatt aagaacaact ttatttatcc aggtcaaacg ttgattgtag
    13021 ctaaataaaa tagctgttcg aagaaataac gattgaattc cacaaaaata tttgttacta
    13081 taaggctact taaatatgac aacacttgaa aaggaagtag taaaaagact gaagtttcag
    13141 agagcgcatg gctggtgaga atgtgcacgg atgcttttga actcgccttt gagttgccct
    13201 tgctgaatac ccgagtaagc aagcggcggt gacggccgtt acaacgttcg aggccttttc
    13261 actaaggtaa aattaggtgg taccgcgtag attacgtcct ataaaaagag caatcttttt
    13321 ataggatttt tttatttctt ttgtgaagtt tgagaaaaca aagcaaaagg aaataaaaat
    13381 aaacagattc tgccggtttt ttcgttatat ttataataga aacaagtgtt ttcaatagaa
    13441 caggaggaca gcaaatgagt tacaatcata aagaaattga gaaaaaatgg caaaaatact
    13501 gggcaaaaac gaatactttc aacacccatg atgatcctga aaagccaaaa ttttacgcac
    13561 tggatatgtt tccctatcca tccggtcaag ggctacatgt agggcatcct gaaggttata
    13621 cggcaacgga tatcctttca cgtgtgaaaa gaagtcaagg atacaacgtg ctgcatccaa
    13681 tggggtggga tgcattcggt ctgcctgctg aacaatatgc gctagataca ggaaacgatc
    13741 cagctgagtt tacccaaaaa aatatcgaaa cattccgacg ccaaatcaat tctttaggat
    13801 ttagttatga ctggaatcgc gaagtgaata caactgatcc ggagtattat aaatggacac
    13861 aatggatctt tacgaagctt tatgaaaaag gtctagctta tgaagcagaa gtagcggtaa
    13921 actgggtacc tgaattagga acagttattt caaatgaaga agtgatcgat gggaaaagtg
    13981 aacgtggtgg ctatgatgta gtcagaaaac caatgcgcca atggatgctg aaaatcactg
    14041 cttatgctga tcgcttattg gatgatctag aattagttga ttggccagaa agcattaaag
    14101 aaatgcaaag aaattggatc ggtcgttcag taggagcaaa tattgaattt aaagtggctg
    14161 gtacggataa atcctatacc gtatttacga cacgaccaga tactctattc ggtgcaactt
    14221 actcggttct tgcacctgaa ctagatcttg ttagagaaat tacgacccct gaacaaaaag
    14281 ctgctgtaga agcttatatc gaagagacag ctaaaaaatc agatctaaag agaacggatc
    14341 ttgcaaaaga aaaaacagga gtatttactg gtgcttatgc aatcaatcca gttaatggca
    14401 aggaaatccc aatctggatc gccgattatg tattatcttc atacggcaca ggagccatca
    14461 tggctgttcc agcacatgat gaacgtgact ttgaattcgc tcaaactttc ggactagaaa
    14521 ttcttccagt tatcgaaggt ggagatgttg aaaaagctgc ttacacagaa gatggtacgc
    14581 acatcaactc tgaatttcta gacggcatga acaaacaaga agcaatcgat aaaatgaatg
    14641 aatggctaga agaaaatggc gtagggaaaa aagaagtcag ctatcgctta cgtgactggc
    14701 tattctcccg tcaacgttac tggggtgaac caatcccaat catccattgg gaggacggaa
    14761 cagtcacagc tgtcccagaa gaagagctac cattacggtt gcctaagaca aaaaacatca
    14821 agccaagcgg tactggagaa tctccattag caaatatcga ggagtgggtc aatgtcgttg
    14881 atcctgtaac aggtaaaaaa ggccgtcgcg aaacgaatac aatgccacaa tgggcaggaa
    14941 gctcatggta ttacctgcgt tatatcgacc ctcataacaa aaaagaacta gcagattatg
    15001 aaaagttgaa gcgttggcta ccagttgaca tctacattgg tggagcagaa catgcggtac
    15061 ttcatttact atatgcacga ttctggcata aattcttata cgatatcggt gttgttccta
    15121 caaaagaacc attccaaaaa ctatacaatc aaggaatgat cttaggcgaa aacaacgaaa
    15181 aaatgtcaaa atctcgtggt aatgtagtca atcctgatga tgtggtcgaa acttacggtg
    15241 cagatacact gcgcatgtac gaaatgttca tggggccatt agatgcttcg attgcttgga
    15301 gcgaaaatgg cttagaagga agccgtaaat tcttggatcg tgtatggcgt ttgatcgttg
    15361 atgagaacaa taaaatgcgt gaccgtatca ctacattgaa tgatggcaag cttgataaag
    15421 tgtatcatca aacagtgaaa aaagtgacag aagactatga aaatcttcat ttcaatacag
    15481 caatctccca attgatggtc tttatcaatg aagcctataa agtggacgct ctgccttatg
    15541 aatatatcga aggatttgtt caactactcg caccgattgc tcctcacatg ggtgaagaac
    15601 tatgggcaat ccttggaaat gatggtggta tttcttacgc accatggcca acatacgatg
    15661 aagcagcact tgttgaagac gaagtagaag ttgttttcca agtaaacggt aaagttcgtg
    15721 cgaaaagcaa tgttccccga gacttaggaa aagatgagct agaaaaagca gcattagcca
    15781 atgaaatagt gcaagaatat attgaaggca aaacagtcag aaaagttatt gcggtaccaa
    15841 acaagttagt caatatcgtt gctaattaat tggctctttg tcaataagga ctgatgagtt
    15901 gtgcaaaatc aaatctgggc cagaatgaac ttcattctgt ctcagatttt ttgttatttc
    15961 aaattgatta attgattgat caaaaaggtt ttgattttca tgttctcaaa cacgttcaat
    16021 gtctttttct tctgaatggt cttgtaagag atctctgtaa tcatcaaatt tttgtctatt
    16081 aatcttagta ttttttgatg tataaactac ctgaaattga gagtacatac caaattcata
    16141 aattttttat tagtgggact agtgattgta tggattttga cgcataatga atgaagtatg
    16201 agaaaaggag agacaaatgg atcaagaact atttattatc gaagtattat cagctaaaca
    16261 aaaaattatc gagaaagcga aaaaaagagc gaaaagaata atgttcaagc ttttaagctt
    16321 agtatttttc tctggtttgc tttttttcgg agctgtatcg ttcctatatc gccatatgag
    16381 tgcagtagtc cgttcagctg aagggatgca aaaattaaat aatttgtatc acttttcgtg
    16441 gggagttggc ggaataacaa ttgttgtgct tattgttttt cttctactta tttggctccg
    16501 ttaccatcaa ttgtcgcgtc atttcgatca atggcttagt caaagagcac aacagttttt
    16561 acataataaa ccaacttctc aagatgatga ctatctttat tttgagaaga cttcgacgag
    16621 tcaagctttt cggatacaaa aaagtgattg ccagttattg ctgagatcac ttgatgattt
    16681 acctatctac ttaggcgaaa gcaaatcaaa attcggaatg aacacattcc atattttttt
    16741 actagaaaac gaacccagat gggaaaaaag ccagtcttcg cctggtggat acctgacaac
    16801 taagatgagt acattagtca ctggtttttt cctcattctt ttagctggaa caatctatta
    16861 tgggagatac actcagccaa aaatgtcttc tcatataaat gctgcttctc acatgacaat
    16921 gaactcaact aacgatacgc atatacaaaa gacaaatgaa gggttgttag ctgatcctaa
    16981 gcaaaaggta aatttgacaa cacaaaagat aaatgatctc aaacttgata cagagacgca
    17041 ctctctctat atgacaacag atagtggtgc taattgggta tttgtgccga ttgattcgca
    17101 atggttgcgt tttggcgaat acaccctgac aacggggctg attcctgaag gttattggat
    17161 ggacaacaca tatgatattt ccgccgattt ctcatggttt atctattctt ctggggatga
    17221 tgtacgtctg ctgacttcaa gagataatgg taaaacatgg caaacgtcta ttatcaccca
    17281 atcagttcag ccgatacgtt atcgaaaagc ggtattttat ggcaacgatg gtctatttat
    17341 ctgtacatca gccagtggga tgtctgcaga aactttgtac atttacacta ctatggatgg
    17401 cggaatcact tggataaaaa gtggaagtac aatgatcaat cagccagtac aaaatgtcag
    17461 cttcgttaca acttctttag gttttgtttc gacacgtgaa agtttgtttt atacaaacaa
    17521 cggaggacgc tcattcaaag aatcagttct gtcgattccg gaaaactatc agacaggggg
    17581 gatagatctt tttcaatcac caaatgaagt tactcaaata agcgcgaata cgttagagac
    17641 aaaattttat ttgttgaaaa aaggaccaat cgatgcaggg aaaatgtttg cctgcctctt
    17701 ccgttcaact gataacgggg aaacatggca attcgttcag caactatcgc aagtagaaca
    17761 aggagattaa agaagagaga ttgtgagcct gacgttcagc accgagtcac aagaaacaaa
    17821 ttcaaaaaac agcttctcat atttgttgaa tagtgtaact cgtgattcgg agttgacaag
    17881 agcaacaaga agcgattttg aagtatagct tctcgtattt tacaagatcg tgtcttattg
    17941 ccgataggtt gtctcttgaa caccgtttat tcgtaaaaag ggattgtgac aagtcttttg
    18001 tcactactta ccgaacgaaa ctgaaggagc tatccaaaaa gtatcattag tattattctt
    18061 tgtgtttcta gtggcaccac tagtacattt aaaaaagcta tcgatcatgg actaatcata
    18121 catgatcgat agctttttag ataatgtttg ttacgctatc tgaaattgac tattataaag
    18181 actggcataa agcgttggtg attggagtag ttcagaatgt gttccttttt cgacgatatc
    18241 gccattcttc attacaagga tcatgtctgc attttcaatc gttgacaaac ggtgtgcaat
    18301 cacaaaactt gtcctatctt ttgtcacagc ttccattgct tgctgaatca atgcttctgt
    18361 tcgtgtatct acgctagaag ttgcttcatc taaaataacg acaggaggat tcgctagaat
    18421 gactcgagca attgtcaata attgttgctg tccttgagat aacgtgccgt tttcgctaga
    18481 aatgatcgta tcatatccgt ctggcagtgt tcgaataaag tgatcacatt gtgcgatttc
    18541 agccgcttcg ataatttgtt ctcttgtggc atccattttc ccgtaggcga tattatcagc
    18601 taccgttcct tcaaacagcc aggtattttg taaaaccatc ccaaaagaac gacgtagttc
    18661 tccacgtgaa agcttggtaa tatcaagacc gtcgaattcg atctttcctt tattcagttc
    18721 ataaaaacgc atcagcagat tgactaaagt cgtttttcct gcaccagttg gaccaacgat
    18781 cgctaccgtt tgttttggct ttactgaaaa atcgacttga ttcatcaata ttttatctga
    18841 tgtgtaacca aactgtacat ttttgaaagc tatttttcct ttcggttgtt ctaaaagtct
    18901 tgcatctgga aggtctggaa tttcatctgt ttcatctaat atctcaaata tcctatcgat
    18961 tgctgccatt gcggattgga tcgagttgat cacataagaa gcggtagaaa ttggttcaga
    19021 aatctgattg atgtactgaa gataagcttg taagaagccg atcgtcacac cacctgatag
    19081 aacaagcata gcaccgaata ccgcacttat gatgaaggcg agttgattaa tgaaacgaat
    19141 ggccggatag atagcacagt tcataaattg agcacgttta aaagcgttgt attgcttttt
    19201 atttacttcg tgtatagcag cttctgcatg agcttgctga ttgaatgttt tgaccactag
    19261 attaccagct aagatttctt ccattcgatt atttaaaaca cccagttcat actgattctg
    19321 ttcagaaatc tgtttgtttt tgttcgcaat cttagttgta acaattgaac tgccaacgat
    19381 cagcaataag acgagcaaag taagcttgct ttcgatataa aacagcatca gaccagcagc
    19441 agctattgtt acgacagaag taaaaaattg attgatacct gtcaataaca cttgagaaag
    19501 ttgattgaga ccggtagttg tccgactaat gatgtcacct acttgatgat tgtcaaaaaa
    19561 cgacatcggt aatgttttga atttttttgt gatttctttt cgcaccctca atgtgatctt
    19621 ttcgcttaac gctgccattg tccgttcctg aataaatgaa gtgatgctgc ttaagactga
    19681 acagccgatc agcaataaga cagggaacaa caatgcttgt ttgatatcac tgaaagaaag
    19741 ctgtccaata cctgtttttc ttatttctga taagagatca tcgatcccga ttcccatgat
    19801 caagggcatg gctacgacta ataggttccc gaataaactg catagaaaca atccgtaaaa
    19861 atatttatgt tcaggtttga tcattttcca aaaacgtatc aaagcacgcg tgttcaacgt
    19921 ttttttcttt tccatcgtat cccttctttc tatattcctt gtgattttgc aaactcctga
    19981 taagtttggc aattagacag tagttcttca tgagtacctt gtccaacgat ttttccttca
    20041 tctaaaacaa tgatgttatc tgcttgttgg attgtactga ggcgttgagc aacaatcacg
    20101 agagttttct ccgacatgtg tttatgaagg gctgctctta atgctgcatc tgttttatag
    20161 tctaaagcgg agaaactatc atcgaataca tagatctctg ctggtttgat caacgctcga
    20221 gcaatacata tccgctgttt ctggccccct gaataattag aaccgccttg agcaacaaag
    20281 ctgtccaatc cgtcaggaag ggtgtccact agttcttcta attgagcgat cttgatcgct
    20341 ttagacaact cttctgttga ggcatattca tttcccatct gcaagttgct taaaattgtc
    20401 ccgctgaata agaaagcctt ctgagggaca taactgatgt gggagcggat ctgttcttga
    20461 gtcatttgag taagaggaat accaccaagg gttacttttc ctatcgtagg gtcactaagt
    20521 ctcaagagaa gtttcgccaa actacttttt ccagatccgg tcccgccaac gatcgctgtt
    20581 gtttttcctt taggtattgt gaaatcaata tcttctagaa ctggatcaga gatttcagta
    20641 tatttaaatg tgacatgttc aaaagcagct gcatttgtct gatcaaaggt tggttcagct
    20701 actgttttga tcgtctcttc ctcaatttcg atctctgctt gcagtatttc ttctatccgt
    20761 cgtaatgagg caaacgattt agggataatg accaatacca tagctgcgat catcaagtaa
    20821 gcaagtgtca gcatagagta ttcgactaca gcagtcacga caccgatttg taatgctcct
    20881 tctgctacaa gatttccgcc aaaccacaaa atagaagcat aaacgattcc catgagcaaa
    20941 aaagcaattg gagtgaccca agcaaatatt cggttcactt tgatcatttg attagcatag
    21001 tcagcaaagc tcttattcgt tcgcttttct tcatatgatt gattatcaaa ggcacggatc
    21061 atattaatcc cagtaaagaa ttctctcaat gtgatggtga tccgatccat ttttggctgg
    21121 atactcaaag ataaaggtgc ccctcgtttc atcagtcgat aaacaatcca tacataaaaa
    21181 acaacagaga tcaatgtaac ccatgttagt aatagagaat aacgaaacat catgagaatg
    21241 gtgaaaacgc aaataaccgg tgctggtaag atcatttggc aaaataaaac aatcatttgc
    21301 tggacattat cgacatcatt cgtcattctt gtcaaaagag atccagaact aaaacgactg
    21361 atatccttca ttgacagctg ttggaattta cggaaatatt tctcccgcgt ttggaatcca
    21421 aacctagcgg aagccaatgc tgaaaagtaa ctagaggcaa ctgcagcgat tgttcctaga
    21481 aacgctacaa gcagcatttc aactccaatt ttttttataa ctgaggcttc gccacttgct
    21541 atacctgtgt cgatcagctt tgctactaat tttggtacac ctaatgtacc ggcgatttct
    21601 aaacagatca tcacaaaggt aaggagagcg agtcctttgt ttttctgtaa gaatgttttt
    21661 aagatagaca tattattcct ccttttttcc tgatactttt cgattctata ccttcaagta
    21721 acttgaaggt caatagaaaa aagaataaac tgaaaaagag gtgagaaaaa atgaaaaaaa
    21781 tgtatacgat tggccaagta tcggaactat ttgatttgcc aaaatcaacg atccgctatt
    21841 gggatgaaca aggattaatc cgttcaagta gacaagaaga gaatgattat cgattgtttg
    21901 atatcgatga tatttttatg ctttatgata tcgtttttta tagaaaatta gatataccga
    21961 tcaaacagat gaagaattta tatggaaaaa cattgacgga gttatatgag acacttgatg
    22021 aaacagaaag aagaatccat aaagaactgg tggtgatgaa acaaaaacaa aaagagataa
    22081 gggagcaaaa aaaacagttg aaattgatga tcgatacaaa cgaggaagaa tttcctgtag
    22141 aagagatccc ttttgactgt atgatctcaa cggagtttga agatattgtg gaaatcaaaa
    22201 aatttctacc taactattca agctttggga tgatgagtat gccagcttcc agttcacaga
    22261 cgatgtatgg attttttatc gaccctagtg aagtacattt atttactcaa gacgtgattt
    22321 gggagaaaaa agatacggct gtttatcgtc gattcttatt gaaatccgag atgaaccatg
    22381 cggaacgtaa taatatttta gaaatcagag aaagaatgta tgaaaaagga tggaaaacag
    22441 gagaagtgat cggccagtat ctattgacga atacggatga aaacaatatc agaactgaat
    22501 attatcatgc atggatagaa atgaaaaaat aggaaatatg tgtttaagat aactcgaaaa
    22561 tgatggttgt tttagttata aagaaaaatt ttctggttcg attacgggtt taatgtgggg
    22621 gattattagt cgtggataga tagaatttta gttttttaga ggaaatatgt ctttaaaaat
    22681 ggaatggttg caattcttag cgatatatac gatgtaattg tcctcttaaa aaagtatgga
    22741 aaaataattg tattgggtgt tatgtataat tcatggtgaa ctgagactaa tgagaagaaa
    22801 aattcatatt gttatgagtg agattgtatc agatgagaat gataaattaa ataggtgcat
    22861 aaaagcatta cacttaaaat acattgttgc ttacattatt acaatatgtt aataaggcaa
    22921 aaataaatag atggattgaa taaacatatt ttttaatcaa atagtcccta ctgtgacttt
    22981 cctcagctat cggtgatact accgatagca tttttttgct tataaaataa ttataagtat
    23041 ttagagattt ttcttctaac attcttcaaa caagaaaaat cacaaaggtt attgttattt
    23101 ttatatattc tttttaagaa tgattgtttt tttatagaaa ttttaaaaaa taaaacacgt
    23161 tattgatgga tagttagttt gttatttggt aatattatag aagttgactg gtagtgattt
    23221 atttttcgtc gttttataaa agtgttattt tttggaaaaa ttcacaaata tgtaacgaat
    23281 gtttttctgg aatttgctgt gttaaaaagc agtatgacta atataaatac tattgtcatt
    23341 tatgcaattt tttatggata gtaattttag aggggaatca atgaaggaag aatagaaggg
    23401 aaaaatagtt gcaaatacgc cagttagcaa aaatagcaat acatcttcaa gctttaaaat
    23461 tcgagagaat catcttggta gtggaatcaa ctacaacaaa aggattaaac caattaatag
    23521 atagaattac caatggaaaa atagaaaagg tagttattct atacaaaagt tgtttaattc
    23581 gcttcggtta tgaacttagt gaaacccttt gcaatatatg gtacagaata tagaaattat
    23641 tgacattaca gaaaatgatg aagatttaat acagattgtc actatttgta gatacctttt
    23701 acaaggaaac cgagccaata aagctaagaa gatgattaag gagttgatgg aagacgatac
    23761 aagttaagaa agtgaaacta cgcccaaata aagagcaaaa agagtaattg gacgtggaaa
    23821 atgcaagaaa tgaaccatcg attcggtgga cagtttatta gtaatgacgg tttgagaaca
    23881 tccatgacga gaatgaagga acatcctaaa tgcgtttagt tgaaccaagt gccaaataat
    23941 atggccaaat aagcgatcag ttatcgatga atcccaagta ttctaaacta tatattcatt
    24001 ttgatgctaa gcattgatat atttttttgg gattgatgaa ggttaaccaa tacagggatt
    24061 cactgatgaa attctagagt ctgaccttgg tatcaacgat ttagtgattg tttctaatgg
    24121 aaaagtataa aagtatataa gtacattaac aaaataacta ttagggttac gctccagctt
    24181 gtgcaaggca ctggcaggag gaatcgtgaa atcataatta tggttaagcc acacggacaa
    24241 ctaaaagtca tacaaagcta tcgaatcaat caacagaaga caatcggcaa tcattagctt
    24301 tgtttacagc ttacattgca acgcttgagt tgaaccgaaa atacgaggga ggtaacgcag
    24361 ccattttgag ttggggaatc tttttagaaa attatcgggt accttgttgt ttagtcaaaa
    24421 ttataagggg gagtaatagt gaggaacaaa tttcataaac tgttaagtat gtttctagtt
    24481 atgcttattg tgatcaatca atttgggaac ataacggttc tagctgagtc tttagcagaa
    24541 cagccagagc gttcgctgac gatcgaagtt gatcaagagg aggctgcttt gggtgagcag
    24601 ttccagttgc gagtcagagg aacaccagac ctgctaaatg cattggtcat tgataagagt
    24661 gaggatttga ttgaagggga agtgaagcaa ctcaatgatt acgaacgtct gattacttat
    24721 tcttcccagc attcaggtaa atacgaattg accgcaagaa gcggtgatat tcaatcaaat
    24781 acggtctcaa tagaaatcag tgtaccagaa gaaagcacaa gtgagtcttt taatgtacca
    24841 gaagaaagca caagtgaatc ttttaatata ccagaagaaa gcttaagtga atcttttaat
    24901 gaagccagat catctgagcc atctataacg caggaatctc agattgaaag acaagaggag
    24961 ctcttgaatt atgacatcgg catctctgct gagcgcccca ctttgccgag caggcataca
    25021 caaacgttaa agggatacat tcaaggaggc gctggcgata ttcttgaaga atatggggag
    25081 atggtcgtac gagttcctag agcatcgatt ggcttaattg agttcattag agtggaggtc
    25141 gctgatgatt cacctttaaa gccactggca ttttaccaag aaggcacaga cgttgttttt
    25201 ccatttacct ttgattatga aggattaaat gaagccgctc agtcgtatac attcagtatc
    25261 acgtttgaag ttgctttctt tcaatcaggc ggtactgagc caaataatga ctttcaagtg
    25321 agtcttactt atgctgagcg agcagcaaac acggaatttt caattacgcc tattccttca
    25381 tcaagaccca ttttttcgaa attatactat ggctctagag aaatcgacag ccaaggaaat
    25441 cttgggaatc tgctgattga cgggcaagac gtgaccaaca gtaataaatt tattttgcag
    25501 gtaaattatg aaggatcacg aaaagacggt gtagtggtta ccgatgtact accgcaagga
    25561 acaactttgg caccgaatcc gactacatgg agttccactg gaaatagaga acctatttca
    25621 ggtggggttc ggatcgcaga agtaacgaat ccaggtgaag caaacgttag aactcattat
    25681 gtttccgagg aatttgtgga caaagtatct tttaataagg aaacgaatac acttgttgtg
    25741 gactttggta atctagcaga gggtcaatcg tattttattg aatatgctct tgaagtgacc
    25801 gatgtcgact tgttcaatga aggaagacaa gaggatgggt tttctcggaa tcgagcgagt
    25861 atgacctaca ataatcaggt aatgagccga gactaccgct tgagaatccc tgaaagtaat
    25921 cttccggcaa tctctttaag aaaaagtgtg gacaaaaatg ttatcaatct caatgaaaat
    25981 gaactaacgt atactctgca tgtctcgatg ttttctggaa caagaaatac gcttgttatt
    26041 gaagatgttt tggaagaagg catggaattt gtgtcatttc tatcagatat tccctctttc
    26101 attgatgccg aagtcaataa tggaatagtt cgtttcagtg cgaataatac gattggtggt
    26161 ccgacaagtt ttaccctctc ttttaaggtc aatgtagagg cgtatgcggt cggcgatcaa
    26221 attttaaact ttgcaacgct tgtgaatagt gacaatcgat tgaatacgag tactgtaaga
    26281 acaaggaaaa tcgatgggcg tattaaagta attaaaactg atgagaatgg ccaaaggctg
    26341 gcaggagcaa aatttgaaat tttatcaagc gattcacaag tgatacaaga aggtataacg
    26401 gcagaaaacg gagaattttt aagtgaaccg cttcctgtag gtagctatca agtccgagaa
    26461 acagaagcac cggaagggta tcttttagat gcgacggtac atgatgtttt tataaatgaa
    26521 tactccatag aagaagtgac gttaaccatt gaaaacatcc ttgatacggg tggtgttgaa
    26581 ctaattaaac atgctgaagg cagtggtgaa gtcct
//
LOCUS       DACDXO010000033        25787 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E23605_2017
            SAMN12639989-rid9881463.denovo.033, whole genome shotgun sequence.
ACCESSION   DACDXO010000033 DACDXO010000000
VERSION     DACDXO010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639989
            Sequence Read Archive: SRR10023389
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741914.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:42:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,972
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 33 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 17 of 73
            Pseudo Genes (multiple problems)  :: 10 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25787
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639989-rid9881463.denovo.033"
                     /isolate="E23605_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            438..992
                     /locus_tag="IWM35_001595"
     CDS             438..992
                     /locus_tag="IWM35_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP9919454.1"
                     /translation="MSYNVQPLRTNEEIQDFQFWLRRTSNPERDSFLFLFGINNGLRM
                     SDIIGLKVGDIRGKSKPIIVEHKTGKRKPIFIDNLREEILLYTEGKEENDWLFPSRQQ
                     GRHITRDRVYQIYADIAEKLGRDDIGTHTLRKTFGYHYYKKTRDIATLMFIFNHSSQA
                     ITKRYIGITEDEIGASLRGFKLGV"
     gene            complement(1165..1638)
                     /locus_tag="IWM35_001596"
     CDS             complement(1165..1638)
                     /locus_tag="IWM35_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP9919455.1"
                     /translation="MRIKPIKDGEQLLFSSEENKPLYLDYVNHNLKIIIKENNLKHIT
                     LHGFRHTHCSLLFESGASVKEVYVRLGHTDIKTTMDDIYTHIAKKQTEETAIRFADFM
                     SSTEEKQGSLQKVKSMVKNEKSRLSLLDSLLNVELSVFFLFRSVCVFETVLILFF"
     gene            1650..2186
                     /locus_tag="IWM35_001597"
     CDS             1650..2186
                     /locus_tag="IWM35_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAP9919456.1"
                     /translation="MPARIHHISIVNRFIRPTFDFYHNILGLKLLMKTINQDDHTMYH
                     LFFSDNHHRVGTELTFFEVQEGNNQFFGTNTIERTILKVPSEASLHFWEIYFETQGVC
                     HYGIESFSGRPILRFEGPDATRLALVPLREFEDIDDYFPYVTDQIPTEHAILGIDAIQ
                     LRVQYSDATERELLSVGW"
     gene            2226..2597
                     /locus_tag="IWM35_001598"
     CDS             2226..2597
                     /locus_tag="IWM35_001598"
                     /inference="COORDINATES: protein motif:HMM:NF013098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAP9919457.1"
                     /translation="MTILENQDTQFYQEVQIIQDRENPVAIEGIGSVHHVAFGVENKS
                     DLQRIDKQLQERNFINSGIKDREFFVSLYYRDPNQLLIEIATGEGNLDAKAYENQSPR
                     FEEIPLFLPQYLNFIREQVEQ"
     gene            2881..4152
                     /locus_tag="IWM35_001599"
     CDS             2881..4152
                     /locus_tag="IWM35_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HAP9919458.1"
                     /translation="MKLSTQEKQLKNVSLKIPKNKISIFTSVSGSGKSSIVFDTITQE
                     AGRQLNDTYDSFTRLFLPKYSRPEVDEIDNLSTAIVVNQKKIGGSARSTLGTITNINA
                     LFRVLFSCFATPSLGYANAYSFNDPSGMCSVCQGIGKTIAFLIGLLYFSAIDNADFVF
                     SDPFTAEVLSGIEEQAKNHGYFVLVHNVNSAEDVKTIQRNWRFEGFIVVGVRVKEFLE
                     LNNVFDGPVVYIDTHLSKETLRELEQQMNRLFVNTNDYQASTLAVEHLIHYGHEKIAF
                     LSFDYIVGEPSVIQERHQAYIDTLKKYHLTFKSHWIYNNSEFERIYQELAEFTAIVVT
                     GDYLAAKLVKFLKNKNKRVMEQLSIIGFDDITFAELMDPALTTIRLNPGNKGAIAFHQ
                     LIGLAKQEEIKNQVLLLEGELISRDSVHKIK"
     gene            4397..5422
                     /locus_tag="IWM35_001600"
     CDS             4397..5422
                     /locus_tag="IWM35_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP9919459.1"
                     /translation="MVGIRDVAKRAGVSIATVSRVLNEDPTLSVTDETKRKIAESVNF
                     YHYVKKSPVAKNLTNIALITTVSEVDELEDPYFRTIRRGIQVEAEQSKVNLKKIIRLS
                     ETPLEQDDLLKCQGVLIIGQVLSSVIEEVSAINSNVVVIDDPSIEAEVDAVYTDLTKA
                     TNQHLDRLYKKGHRNIAFIGGGRVRLDSQGNKHVSDDDYRKIAYETWMKQKGLEKYSQ
                     IYLEGWTTLDGMQAADLLLKKHAKDKLPTAIMVGNDPIAVGVYRSLQKNGLHIPEDIS
                     IVSFDNIEVAEFLTPALNTVNVNTEEIGKLAVRMVNERIKEVRNVSIKVIVSNNIIVR
                     ESEKNID"
     gene            5716..6825
                     /locus_tag="IWM35_001601"
     CDS             5716..6825
                     /locus_tag="IWM35_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAP9919460.1"
                     /translation="MLDEFEKQNPDIKVEMETTPWDQYWVKLEAATTGGNMPDVVTMH
                     SSESYKYMSQNALMGLDDVIKENDIDMGNFTEGIADFYTLEDELYAIPKDASVVGLWY
                     NKELFDNAGVDYPDETWTWETLRQAAIDLTDESKGIYGFASHIGTETGYWPFIYQNGG
                     EVFADDNTKSGLNTPEAQEALAFYRDIALVDHASPTITELQDPDKLSRFQSGRLAMMI
                     EGNWFAPGFLKNDYLVENVASAVLPKGKVNATITNGLGWAASASTKYPEEVKKLMAFL
                     ASEDANKIQAETGASIPALKGFEDEWAASSDTIDLSAFGKMLEYGVPRPYNKDGLRAE
                     QAEVQIINRILSGEVTVEDGTAQIEKEVNAILSGN"
     gene            6908..7804
                     /locus_tag="IWM35_001602"
                     /pseudo
     CDS             6908..7804
                     /locus_tag="IWM35_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008378728.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
     gene            7820..8644
                     /locus_tag="IWM35_001603"
     CDS             7820..8644
                     /locus_tag="IWM35_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002579255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP9919461.1"
                     /translation="MKNKFFSNAPIHIMLILGGIAMALPFIWMILTSGKTLVESTQIP
                     PTIFPEKFQLSNYQQVLDLLPFMAFYINTFLMVVGRVIGSVLFSAMAAYACARLSFPG
                     KNILFSLVLIQMMIPGSIFITPQYLLAQKLGILNTIPALILPGVVSAFGTFLLRQFFL
                     GLPDELEEAAYLEGANIWTIFFKIMLPLAKSGLVALGIFTALFAFKDLMWPLIVNTSI
                     DKMPLAPGLANLQGQYATNFPQLMAGSLLAIWPIVVIFIVFQRKFIEGIATTGSKL"
     gene            8676..9986
                     /locus_tag="IWM35_001604"
     CDS             8676..9986
                     /locus_tag="IWM35_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005235059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase/alpha-galactosidase"
                     /protein_id="HAP9919462.1"
                     /translation="MTKITFIGAGSTIFAKNVLGDAMLTPSLQDAHIALYDIDEKRLK
                     ESNLMLKNINKNSNENRADIVSYYDRKEALREADFVINAIQVGGYKPSTVIDFDIPKK
                     YGLRQTIGDTMGIGGIFRTLRTLPVMLDFAKEMEEVCPNAWLLNYTNPMAMLTMGVLK
                     ATSIKTVGLCHSVQVCVPELFEHLGIADQYDLNDFQWKIAGINHMAFLLEITKNGEDF
                     YPTIRQLAAEKPNPHPDSVRFELLKRFGYYITESSEHNAEYHPYFIKSKYPELIDELG
                     IPLDEYLRRCEQQINDWESMREDIVNNGELTHTRSREYASYIMDSITTGTPSVIAGNV
                     LNKGLITNLPTDVCVEVPCLVDKNGVQPTYVGELPTQLAALNRTNINVQELTVEAALT
                     LDKSKIYQAAYLDPHLSSELSLTDIKNLVDDLIEAHGDYLPEYN"
     gene            10134..11393
                     /locus_tag="IWM35_001605"
     CDS             10134..11393
                     /locus_tag="IWM35_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP9919463.1"
                     /translation="MKWSKKIILACIALGATISLTACGKNQEDASGKVEIEFFNQKKE
                     MTQTIQEIAKDFEAKNPDIHVKVVDVPNAGEVIKTRMLAGDVPDVINLYPQSIELKEW
                     AKAGYLEDLTEEPYLENIKNGYAQRFAIEDRVYSIPLTANVYGFYYNKTAFEEMGIQA
                     PETWAEFEKIVAEIKDQNKVPFAIAGAEGWTLNGYHQLAIATAAGGEEEANNVWRFSE
                     VNGINAESKEMQKDFNRLDLLREPKALQNNWQGAGYNDTVVTFTKGDALIMPNGSWAM
                     PMIHSQNPDFEVGTFPFPADEAGQSLTIGAGDLALSVSATSQHKEEAKRFVEYMTTPE
                     AMQKYYDVDGAPCAVEGVIENTQDSPLSGLTELAFTDRHLVWLAKDWNSENDFYTLTT
                     NYLHNGNQKAMINALNAFFNPMKADGR"
     gene            11403..12275
                     /locus_tag="IWM35_001606"
     CDS             11403..12275
                     /locus_tag="IWM35_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018363968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP9919464.1"
                     /translation="MKIKEILNRYWAIIFVAIPIILQMIFFYFPMVQGAFYSLTNWTG
                     LTYNFDFVGLNNYKILFSDPKFIKSIGFTLIVTLCMIAGQIGFGILIARALNANIKGK
                     TFFRAWFFFPAVLSGVVVSLVFKQIFNYGLPKIGSMLGIEFLQTSLLGTTWGAIFAVI
                     FVLLWQGIAMPVIIFLAGFQSIPQEIIEAARMDGANDKQIFWNIEITYLLPSISMVFI
                     MALKGGLTAFDQIFALTGGGPSDATTSLGLLVYNYAFKNNQFGYANAIALVLFILIGI
                     VSLAQLKLSKKFEI"
     gene            12287..13117
                     /locus_tag="IWM35_001607"
     CDS             12287..13117
                     /locus_tag="IWM35_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP9919465.1"
                     /translation="MKTSKEKNISKYLLLLLGGFMILVPLVSTLFGSFKTTPDIMNNF
                     FRFPNPATLENFRRLLADGIGHYFWNSAIITIIAVVAVTAIIPMAAYSLARKMTRGKA
                     YGLMYLFLILGIFVPFQVIMIPITVMMSKIGLANMWGLILLYLTYAVPQTLFLYVGFI
                     KLNVPISLDEAAEIDGASHFTTYFKIVFPMLKPMHATTIILNALWFWNDFMLPLLILN
                     RDSNMWTLPLFQYNYTGQYFNDYGPSFASYTVGIVTITLVYLAFQRSIIAGMSNGAVK
                     "
     gene            13157..15340
                     /locus_tag="IWM35_001608"
     CDS             13157..15340
                     /locus_tag="IWM35_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HAP9919466.1"
                     /translation="MIIFDNHTQTFHLQNKKISYLLGIEENNYLTHLYFGKKITHYSG
                     GYRYPRTDRSFSPNNANAQDRLLSLDTLPLEFPGYGHGDFREPAHNIKLGNGSRVNDF
                     RYDSYEIQKGKPSLAGLPALYTEDDDEAETLIIQLKDAISGLTLQLFYTIYQNHSVII
                     RSTQLVNNSDETVEINRLASQSIDFPNRPLELIHLNGTWARERQMTREKIHTGIKVLD
                     SKRGSSSHQQNPFVALVDAKTDEFQGEAYGFNLVYSGNHETVIQKDPFDQTRVIMGIN
                     SFNFGWQLKTGDSFQTPEVVMVYSNEGLNTLSQTYHELYNHRLIRGNYRLKERPTLIN
                     NWEATYFDFNEEKIMNIVDDAKNLGIEMFVLDDGWFGGRENDFTSLGDWVETEGKLAC
                     GLENLAKQVHDNGMKFGIWFEPEMISEDSELFRAHPDWALGIPGRDRSISRNQYVLDF
                     SRQDVRDNIYQQMTAIFDRVPIDYVKWDMNRNMTEVYSVLLDSTMQGEVAHRYMLGLY
                     EFLEKLTTNYPTILFESCSGGGGRFDAGFLYYMPQTWTSDNTDAVARLKIQYGTSLVY
                     PISSMGAHVSAVPNHQTGRETSLTIRGNAAMAGVFGYELDLTQLTSEEKAEIKEQVAF
                     YKKHRQLLQYGTFYRLESPFEGNDTAWMFVSKDQTEAMVFYFRVLAEAAHPLVTLKLA
                     GLAEEATYQMEDVTFAGDELMNLGFYIDSELHGDYATQRFYLKQI"
     gene            15401..16621
                     /locus_tag="IWM35_001609"
     CDS             15401..16621
                     /locus_tag="IWM35_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligo-1,6-glucosidase"
                     /protein_id="HAP9919467.1"
                     /translation="MFYLHAFTKKQPDLNWENEEVREEIYAMINYWLDKGLGGFRIDA
                     ILNIKKKLEYGIFESDGEDGLAFIGPWILNQSGIEVWLKEMRERTFKPHNSMTVAEAD
                     VPNERLDEYIGEDGYYSMVFDFSYTDIDVPETGEWFKDSQWTWTDMRTNIFTNQLVTQ
                     DKGWGALYLENHDQPRSINKYIPEEWINDYSKKMLATLFMLLRGTPFIYQGQELGMTN
                     IEMASLEDFDDVATHSQYKRALEYGLAPEQALKAVNKRSRDNSRTPMQWNTQKNARFS
                     TSDNVWLKVNPNYPELNAEAQLTNQQSVFNYYHQLIKLRKNSVYKDVLIYGQFLPVKD
                     KNEHILLYERQLEDKIVLVILNVTATEQEYYVDEQYQQVLINNYEDVLLDQSRKLRLR
                     PFESIVLANYGGQL"
     gene            16618..18078
                     /gene="gtfA"
                     /locus_tag="IWM35_001610"
     CDS             16618..18078
                     /gene="gtfA"
                     /locus_tag="IWM35_001610"
                     /EC_number="2.4.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HAP9919468.1"
                     /translation="MKIKNEAMLITYADSLGKNMGELKQVLDKHLQGVIGGVHLLPFF
                     PSTGDRGFAPSDYTTVDPALGTWEDVEALGEDYYLMFDFMINHISRESKFFQDFKKNH
                     EQSPYKEMFIRIHEFFPENRPTQEDIDLIYKRKDKAPFQTVEFADGTTEEVWNTFGEE
                     QIDLDVTKEVVKEFIRETIKDMANHGCSLIRLDAFAYAIKKLDTNDFFVEPDIWELLD
                     EVRQEAAKYDVELLPEIHEHYSIQMKIANHDYYIYDFALPMVTLYSLYSGKSERLANW
                     LKMSPMKQFTTLDTHDGIGVVDARDLLTDEELDYTSEELYKVGANVKKVYSSANYNNL
                     DIYQINSTYYSALGDNDRSYLLARAIQVFAPGIPQIYYVGLLAGKNDIELLEATKEGR
                     NINRHYYDLAEIEEEVQRPVIQQLFALLKFRNESAAFDLDGTIEVATPSESEIVITRK
                     NKNGSDSATLTANLDTKEFIIESKDIKIFTSVALKF"
     gene            18155..19261
                     /locus_tag="IWM35_001611"
                     /pseudo
     CDS             18155..19261
                     /locus_tag="IWM35_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175341.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            19583..19792
                     /locus_tag="IWM35_001612"
     CDS             19583..19792
                     /locus_tag="IWM35_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAP9919469.1"
                     /translation="MVVITKNELMKLGYGKYQSERIIRQAKAIMVSKSYTYYENRRLG
                     LVPVESVEEVLGMKIEVEEDFQNHG"
     gene            19785..19934
                     /locus_tag="IWM35_001613"
     CDS             19785..19934
                     /locus_tag="IWM35_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HAP9919470.1"
                     /translation="MVNFKKDKNGKYHFTASLGFDKRTGKRVQKNVEGLKQLKKKKSV
                     YRTCF"
     gene            19969..20753
                     /locus_tag="IWM35_001614"
                     /pseudo
     CDS             19969..20753
                     /locus_tag="IWM35_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922412.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            complement(21037..22122)
                     /gene="xerS"
                     /locus_tag="IWM35_001615"
     CDS             complement(21037..22122)
                     /gene="xerS"
                     /locus_tag="IWM35_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HAP9919471.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLDNNPMDQVATITDKTTLAARAN
                     AIEKKLFLDKDTQNYLDYIANEYKNTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VININLDDLSLDKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRIQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHSSTNTTTLYTHIDNAATIDALNAL"
     gene            22456..22689
                     /locus_tag="IWM35_001616"
     CDS             22456..22689
                     /locus_tag="IWM35_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antitoxin"
                     /protein_id="HAP9919472.1"
                     /translation="MAIKERKFREVGNSVVLTISKEFLKENGLNPGDTVFLDEEKLRA
                     AITKKQALSDEIDLAISQALTEYDTTFRELVDK"
     gene            22686..23093
                     /locus_tag="IWM35_001617"
     CDS             22686..23093
                     /locus_tag="IWM35_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HAP9919473.1"
                     /translation="MSIHYLEAKDIIKMNVIQIKKYSPNEPLGVKDANALEMCVGQLR
                     ASAFGEEVYPSIYEKAAILLIQLIKKHPFHNANKRTAFLATFVFLKINGQLLAIDQKD
                     AVNLAVYIATYDKDFDQLKYEVINTIKTHTQPI"
     gene            complement(24230..24748)
                     /locus_tag="IWM35_001618"
     CDS             complement(24230..24748)
                     /locus_tag="IWM35_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HAP9919474.1"
                     /translation="MRTYKNKEELKKEINRTYTKYILEFETIPEELKDKRVEEVDRTP
                     AENLAYQVGWTNLILKWENDERNGLSVKTPSDQFKWNQLGELYKWFTNTYAHLPLKEL
                     EEILNRNIDDINMMIDSMSDEDLFTAHKRKWADEATKTAVWEVYKFIHVNTVAPFGTF
                     RTKIRKWKRLAL"
     gene            25115..25363
                     /locus_tag="IWM35_001619"
     CDS             25115..25363
                     /locus_tag="IWM35_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9919475.1"
                     /translation="MVKLKMLYIGLQHRSFFDCALGKKYYEVKIDDDTFLTAHQMKQL
                     QKRNDQLEENLILKKRLPYSRLARRTIKGQDLPEDHPF"
BASE COUNT         8786 a         3930 c         4795 g         8276 t
ORIGIN      
        1 aattattgac gctcccgaca aaattatgat cttgcgtaaa gttataaaat attaataaaa
       61 atattttatt aatgaaaagc ttggtagcac ggttattcat atgctaattt atttcacttt
      121 atagtaaggg gagttcttag tatattgttc aaacttaaat ttcatataat ttaattatgt
      181 aaattagcta ttctaagaac ttctacggat aactgcccgt ctaaaattta aataagagct
      241 tagaatcttc atataagtaa ggttaatttt tttgtacaaa aaagtcggaa tattctgagt
      301 tactgaaatg atataaaatc ataaactaaa tcccttgatt ttattgactt tcaaaagttt
      361 atatatataa gttaatgatt atggtaaaat atatacataa tcattaactc agaatatttt
      421 taagaaggga aacaatagtg agctataatg tacaaccgtt aaggacaaat gaagagatac
      481 aagattttca gttttggcta agaagaacaa gtaatccgga aagagatagt tttttatttt
      541 tgtttggaat caataatggg ttacgtatgt cagatattat aggattaaaa gttggagata
      601 tacgaggcaa aagtaagcct attattgttg aacataaaac aggtaagcga aaaccgattt
      661 ttatagataa tttgagagaa gaaattttat tatatactga aggtaaagaa gaaaatgatt
      721 ggttatttcc gagtcggcaa caaggtcgac atataacaag ggatcgtgtc tatcagatat
      781 atgcagatat agcagaaaaa ttaggacgag atgatatagg aacacataca ttaagaaaaa
      841 cttttgggta tcactactac aagaaaacta gagacattgc taccttaatg tttattttta
      901 atcatagctc gcaagcaatt acaaaaaggt acattggtat aactgaagat gaaataggag
      961 catcacttcg tggatttaaa ctaggtgtat aatttaaaaa tttttataaa cagtaaagac
     1021 aaagattaaa agctattttt atctagaacg attttatttc ataaagttcg cggaataaaa
     1081 gctggggtta ctggtacttg gtataggttc gtgttatata aactaagtaa ttaaataaca
     1141 tttttaggtt ggaaaaggat gaaattaaaa aaagagaatc aatacggtct caaaaacgca
     1201 aacgctgcgg aataagaaaa atactgataa ctcaacgttt agcagactat ctaataggga
     1261 tagtctgctt ttttcgtttt tgaccatact tttgaccttc tgcaaacttc cttgcttttc
     1321 ctctgtagaa ctcatgaaat ctgcaaaacg aattgccgtt tcctctgtct gctttttcgc
     1381 gatatgggtg tatatatcat ccatagtggt tttaatatct gtatgtccaa gtcgaacata
     1441 cacttctttg acagacgcac cagattcaaa gagcagacta caatgggtat gacgaaaacc
     1501 atgaagtgtg atatgtttta agttattctc tttaatgatg attttcaaat tgtgatttac
     1561 atagtctaaa taaaggggct tattttcttc tgatgaaaac aaaagttgtt caccatcttt
     1621 tataggtttg attctcattt ttagatatta tgcccgcacg tattcatcac atttctattg
     1681 tgaatcgctt tattcgaccg acatttgact tttatcataa tatactcggt ttaaaattac
     1741 taatgaaaac aattaatcaa gacgatcata ccatgtatca cctgtttttt tcagataatc
     1801 accaccgtgt gggaactgaa ctgacttttt ttgaagtaca agaagggaac aaccagtttt
     1861 ttggaaccaa tacgattgaa cgaacgatac taaaagttcc ttccgaagca tcattgcatt
     1921 tttgggaaat atattttgaa acacaaggcg tttgccatta tggaatcgaa tcattttctg
     1981 gacgacctat cttacgtttt gaagggccag atgcaacacg attagcttta gttcccttac
     2041 gcgaatttga agatattgat gattatttcc cgtatgtcac agaccagatc cctacagagc
     2101 atgccatttt aggaattgat gccattcaac tacgcgtaca atattccgat gcgactgaac
     2161 gagagttatt gtcggtaggc tggtagcaca aagaatacgt cagtttcttt tcgggtcatc
     2221 aacgagtgac aatcttagaa aatcaagaca ctcagttcta tcaagaagtt cagattattc
     2281 aagatcgtga gaatccagtt gccattgaag ggattggtag cgtccatcat gttgcgtttg
     2341 gcgtagaaaa taaatctgat ttacaacgca tcgacaaaca attacaagag cgcaatttta
     2401 taaattcagg aataaaagat cgtgaatttt ttgtatcact ttattatcgt gacccaaatc
     2461 aattattgat tgaaattgct acaggggaag gaaacctaga tgcaaaagcg tatgaaaatc
     2521 aaagtccacg ctttgaagaa attcctttat ttttaccgca gtacttaaat ttcattcgtg
     2581 aacaagtgga gcaataatta gctaatcaaa tccatgacga agagtaagag gttgactcat
     2641 aagtcaaaaa ataaagaaaa acgcatcaaa tccattctaa agaatgttga tttgatgcgg
     2701 ttcctatttt tataaactat cattttcgac taataaaact acttatgagt cagcctctct
     2761 ttttttcttg atttttatac ctctttctag gtaaactaaa catctgccaa aaataccgtg
     2821 tctcattgaa acattctcaa ctatttcaaa ataaagggga gtaccaaatg aacgagtata
     2881 ttgaaattat ccacgcaaga gaaacaactt aaaaatgtca gtctcaaaat tcccaaaaat
     2941 aaaattagta tttttaccag cgtatctggt tcaggaaaat catcgattgt ttttgatact
     3001 attacccaag aagctggtcg tcaactgaat gacacctatg atagttttac ccgtttattt
     3061 ttacctaaat acagtcgtcc agaggttgat gagattgata atctttctac ggcgattgtg
     3121 gtcaatcaaa agaagattgg tggcagtgcg cgctcaacgt tagggacgat tactaacatc
     3181 aatgcgttat ttcgtgtgtt gttttcatgt tttgcaacac ctagtttagg atatgcaaat
     3241 gcttattcat ttaatgatcc tagtggaatg tgttctgtct gtcagggaat cggtaagacg
     3301 attgcatttt taataggatt gctttatttt tcggctattg ataatgctga ttttgtattt
     3361 tctgatccat ttaccgcaga agttttgtct ggaattgagg aacaggcaaa gaatcatggt
     3421 tattttgtat tggtgcataa tgttaattca gcagaagatg ttaaaactat tcagagaaat
     3481 tggcgttttg aggggttcat tgttgttggt gtacgtgtaa aagaattttt ggaattaaat
     3541 aacgtttttg atggtcctgt tgtttatatt gatacgcatc tttcaaaaga gacattaaga
     3601 gaacttgaac aacaaatgaa tcgtttattt gtaaatacaa atgattacca agctagtaca
     3661 ttggcagtgg aacatttgat tcattatggt catgagaaaa tagcgtttct ttcttttgac
     3721 tacattgtag gtgaaccaag tgttattcaa gaacgacatc aagcttatat cgatacatta
     3781 aagaaatatc atttaacctt taaatcgcat tggatatata acaactctga atttgagcga
     3841 atttatcaag aattagctga atttactgca attgttgtta ctggagatta tttagcggct
     3901 aaattagtaa aatttttaaa aaataagaat aaacgagtga tggaacaatt atcgattatt
     3961 ggatttgatg atattacttt tgcagaatta atggatcctg cgttgacaac aatacgacta
     4021 aatccaggta acaagggggc gattgctttt caccaactga ttggactggc caaacaagaa
     4081 gaaataaaaa atcaagtact actattagag ggagaattga tttctcgaga ctctgttcac
     4141 aaaatcaaat aaaaaagctc tgtgaaataa gtatagttaa caataaaata tccgaacgat
     4201 agagagaatg aactgtgatc tagcagttcc tcgtcgcaaa ttcactagaa ttagcattat
     4261 ttactctaaa ttgttcggat attttttctt gtttttaatt atgattacag tgcctatttt
     4321 taacgaaatt tgcttatttt caaaatgtat gattatatgt tagtaaacac tttcaaatat
     4381 gaaaagagga gctattatgg taggaattag ggatgttgca aaacgggcgg gtgtgtccat
     4441 tgcaacagta tcgcgcgtgc taaatgagga tccgacactt tcagtaaccg atgaaaccaa
     4501 acgaaagatt gcagaatcgg ttaattttta tcattatgtc aaaaaaagtc ccgttgcaaa
     4561 aaatttaaca aatattgcat taataacgac agtttctgaa gtagatgagt tggaagatcc
     4621 ctattttcga acgattcgca gagggattca agtcgaagca gaacaaagca aagtcaattt
     4681 gaagaaaatt attcgtttgt cagaaacacc gttggagcaa gacgatttgc taaaatgtca
     4741 aggtgtgttg attattgggc aggtgctatc ttctgtgatt gaagaagtat cagcaattaa
     4801 ttcgaatgtc gtagtgattg atgaccctag tattgaagct gaagtagatg cagtttatac
     4861 tgatttaaca aaagcaacga atcagcattt agatcgattg tataaaaaag gacaccgaaa
     4921 cattgcgttt atcggaggag gacgtgttcg tttagatagc caaggaaata aacacgtgtc
     4981 agatgatgat tatcgaaaaa tagcctatga gacgtggatg aaacaaaaag gattagaaaa
     5041 atatagtcaa atttatttag aaggctggac cacacttgac ggaatgcagg cagcagattt
     5101 acttttgaaa aaacatgcta aagataagtt gccgactgct atcatggtag gaaatgatcc
     5161 tattgctgtg ggggtttatc gttcacttca aaaaaatggt ctacatattc cagaagatat
     5221 ttcgattgtg agttttgata atattgaagt tgctgaattt ttaacacctg cattaaatac
     5281 ggtgaatgtg aatacagaag aaattggaaa actagcagtt cgcatggtta atgaacgaat
     5341 aaaagaagtt agaaatgttt cgatcaaagt gattgtttct aataatatta ttgttagaga
     5401 aagtgaaaaa aatatcgatt aaaaaagtaa acatccaaaa aagggtgttt acttttttgt
     5461 ttactaatat ttttagtaaa aaatctcgac aaaacattaa gtaaacagtt atggtgttgt
     5521 taacaaaaag aaaacgcatt caaaacagga ggaattaaga tgaaacattt caaaaaaata
     5581 gctttaacag ggatagctgt tttaactggg acattactcg cagcatgtgg tgggggagat
     5641 agtaattcta aagacgggca agtaacttta agctatgcga gttgagatac tgaccaagct
     5701 ccaggactaa gaaaaatgtt agatgaattt gaaaaacaaa atcctgatat caaagtagaa
     5761 atggagacaa cgccttggga tcaatattgg gttaaattag aagctgcaac aacgggaggt
     5821 aatatgccag atgtagtaac catgcactct agcgaatcct ataaatatat gtcacagaat
     5881 gctttgatgg gacttgatga tgttatcaaa gaaaatgata ttgacatggg aaactttact
     5941 gaaggaattg ctgatttcta tacgcttgaa gacgaactat acgcaatccc taaagatgct
     6001 tctgtagtgg ggctatggta caacaaagaa ttatttgata atgcaggtgt cgattatcca
     6061 gatgaaacat ggacatggga aacactcaga caagctgcaa ttgatttaac agatgaatca
     6121 aaaggcatct atggttttgc atcacatatt ggaacagaga ctggctactg gccatttatt
     6181 tatcaaaatg ggggagaagt atttgccgat gacaatacaa aatcaggttt gaatacccca
     6241 gaagcacaag aagctttagc tttttatcga gatattgcac ttgttgatca tgcttcacca
     6301 acaattacag aattacaaga tcctgataag ttaagtcgtt ttcaatcagg tagattagca
     6361 atgatgattg aaggaaactg gtttgcaccg ggatttttga aaaatgacta tctagttgaa
     6421 aatgttgctt ctgccgtgtt acctaaggga aaagttaatg caacaattac aaatggactt
     6481 ggttgggctg ccagtgcgag tacaaaatac ccagaagaag tgaaaaaact aatggcattt
     6541 ttagctagtg aggacgccaa taaaatccaa gcggaaacgg gggcatcaat cccagcacta
     6601 aaaggctttg aagacgagtg ggctgcttct tcagatacaa ttgatttgag tgcttttgga
     6661 aaaatgttgg agtatggagt ccctcgtcca tacaataaag atggattacg agctgaacaa
     6721 gcagaagttc aaattattaa ccgtattctg tctggtgaag taactgttga agatggaacc
     6781 gctcaaattg aaaaagaagt caatgcgata ttgagcggaa attaaattag ttaattatac
     6841 aatatacagg cgaaaggatt ctatttcgtc tgtatattaa tggatgaata ccaaaaaggg
     6901 ggatattatg aaaaatcgaa tttttggaaa atatcgtaaa gatcaagtga tatggggcta
     6961 tatttttatt tttccaacgg cattaggatt aattttatta aatatatggc cagcaattca
     7021 aacagcaatt ttaagttttc aaaaaacggt agggttcggt gatactgaat gggtcggatt
     7081 aagaaattac caaaaaatgt ttgcagacgg tgaagttttt cagtctttat taaacacatt
     7141 aatttatgca gtggtttcga tacctagtat tgttgtatta tccttgttgg tggcagtttt
     7201 aatgaataga aaagttaaag gattgtcgat ttatcggaca atttattttc taccagtcgt
     7261 tgctgcacca tccgcagtcg caatgatttg gcgctggatg ttcaataatg attatggact
     7321 gattaataac atgttggcta aaataggtct cgatggacca gcatggttaa cggatccatc
     7381 gattgcgatt tattctatta tcattgtagg agtttggagt gctattggtt ataacatggt
     7441 gttattacta gccggcttat aagaaattcc taaagattat tatgaagcag cggaaattga
     7501 tggtgctagt ccaatccagc agtttttcaa cattacgtta ccattagtta caccaacatt
     7561 attttttgta gttgtaacaa cagttatcaa tgctttccaa gtatttgatg ttatttttat
     7621 gatgattact cctaatagta ccgccatgtt taaatcacaa tcattagcct atttattcta
     7681 taaacattca tttattttaa atgacaaggg atatggttcg gcaatcgtta tgttcctttt
     7741 agtgattata ttaattatta ctgcaattca aatgaagttg cagaaaaagt gggtaaacta
     7801 ttaaggaggc tatcaaatta tgaaaaataa atttttttca aatgcgccaa tccatatcat
     7861 gcttatttta ggtggtattg caatggcatt accttttatt tggatgattt taacttcagg
     7921 gaaaacgttg gttgaatcta cccaaattcc acctacaatt ttccctgaga aatttcagct
     7981 aagtaactat caacaagtac ttgatttatt accattcatg gctttttata tcaatacatt
     8041 tttaatggtt gttggacgag tgattggctc ggttttattt tcagcaatgg ctgcatatgc
     8101 ttgtgcgcgt ctaagttttc cagggaaaaa tatccttttc agcttagtac ttattcaaat
     8161 gatgattccc ggttccattt ttattactcc acagtattta ttagcacaaa aattgggtat
     8221 tttaaataca attccagcat taatattacc aggggtagtt agtgcgtttg gtacgttttt
     8281 attaagacaa tttttcttag gtttacctga tgaattggag gaagcggcct atttagaagg
     8341 tgccaatatt tggacgatct ttttcaaaat tatgttgccg cttgctaaat caggattggt
     8401 tgcacttgga atattcacag cgttgtttgc atttaaagat ttaatgtggc cattgattgt
     8461 caatacttca attgataaga tgcctttagc tccgggatta gcaaacttac aaggacaata
     8521 tgccacgaac ttcccacaat taatggcagg ttcgttatta gcaatttggc caatagttgt
     8581 gatattcatc gttttccaaa gaaaattcat tgaaggaatt gctacaacag gtagtaaatt
     8641 ataaaactat taattagtga ttggagagta agtacatgac aaaaattaca tttattggtg
     8701 caggaagtac tatttttgca aaaaacgtat taggcgatgc gatgttaact ccaagtctac
     8761 aagatgcaca tattgcattg tacgatattg atgaaaaacg actaaaagaa tcaaatttaa
     8821 tgcttaaaaa tattaataaa aattcgaatg aaaatcgtgc tgatattgtt tcttattatg
     8881 atcgcaaaga agcgttaaga gaggctgatt ttgttatcaa tgctattcaa gtgggtggtt
     8941 ataaaccttc aacagtgatt gattttgata ttcctaaaaa atatgggttg cgacaaacta
     9001 ttggtgacac catggggatt ggtgggattt ttagaaccct acggacacta ccagtaatgt
     9061 tagactttgc taaagaaatg gaagaagttt gtccaaatgc ttggttattg aactatacaa
     9121 atccgatggc gatgttaacc atgggtgttt taaaagcgac gagtattaag actgttggtt
     9181 tatgtcatag cgttcaagta tgcgtaccag aattgtttga gcatttagga atagccgatc
     9241 aatatgactt aaatgacttt caatggaaaa ttgcaggcat taatcatatg gcattcttgt
     9301 tggaaattac aaaaaatggg gaagatttct atccaacaat tcgtcagtta gctgcagaaa
     9361 aaccaaatcc acatccagat tctgtacgtt ttgaattgtt gaaacgtttt ggttactata
     9421 ttacagaatc aagtgaacac aatgcagaat accatccata ctttattaaa tcaaaatacc
     9481 cagaattgat tgacgaatta gggattccgt tggatgaata cttacgtcgt tgtgaacaac
     9541 aaattaatga ttgggaaagc atgcgagagg acattgtaaa taatggggag ttaacacata
     9601 cgagaagccg tgaatatgct tcatatatca tggattctat tacgacagga actccgtcag
     9661 tgattgcagg aaatgtctta aataaaggct taatcacaaa cttaccgaca gatgtttgtg
     9721 tggaagtacc ttgtttagtc gataaaaatg gcgtgcaacc aacctatgtc ggtgaattgc
     9781 caacacaatt agctgcttta aatcggacga atatcaatgt tcaagaactg acagtagaag
     9841 ccgctttaac actagataaa tcaaaaattt atcaagcagc gtatttagat ccacatctat
     9901 catctgaatt gtctttaaca gatatcaaaa atttagtgga tgatttaatc gaagcacatg
     9961 gggactattt acctgaatat aattaaaaag aagctgaaag agtaaggaat tttcaattcc
    10021 ttactctttt cttagttaaa tttagtaaaa atattgacaa aacacttagt aaacagttac
    10081 attagtgtta acaaaaagaa aacgcattca aattgtataa ggaggagcat aaaatgaagt
    10141 ggtctaaaaa aattatttta gcttgtatag cactaggagc aacgatctct ttgactgctt
    10201 gtggaaaaaa tcaggaagat gcaagcggta aggtagaaat agagtttttt aatcaaaaaa
    10261 aagaaatgac tcaaacgatt caggagattg ctaaagattt tgaagcaaaa aaccctgata
    10321 ttcatgtaaa agttgtggat gtaccgaatg ctggtgaagt aattaaaaca cgtatgcttg
    10381 caggcgatgt accagatgtc attaatcttt atcctcaaag tatagaattg aaagaatggg
    10441 cgaaagctgg ttatttagag gacttaaccg aagaaccgta tttagagaat atcaaaaatg
    10501 gatatgcaca acgttttgct attgaggatc gtgtatatag cataccatta accgctaatg
    10561 tctatggttt ttactataac aaaactgctt ttgaggaaat ggggattcaa gcaccagaaa
    10621 cttgggcaga atttgaaaaa atagtagctg agattaaaga tcaaaacaaa gtaccgtttg
    10681 cgattgcagg agcagaaggc tggactttaa atggttatca ccaactagct atagcgactg
    10741 ctgctggtgg tgaagaggaa gcgaacaatg tttggcgttt ctcagaagtt aatggaatta
    10801 atgctgaaag caaagaaatg caaaaagatt ttaatcgatt agatttgtta agagaaccaa
    10861 aagctttgca aaacaactgg caaggtgccg gttataacga tacagtagtt acttttacaa
    10921 aaggtgatgc gttgattatg ccgaatggtt catgggcaat gccgatgatt cattcacaaa
    10981 atccagattt tgaagtagga acttttcctt ttccagcaga tgaagcaggt caaagtctaa
    11041 cgattggcgc aggagaccta gccttatcag tttctgcaac ttctcaacat aaagaagaag
    11101 ccaaacgttt tgtagaatat atgacaacac ctgaagcgat gcagaaatat tatgatgtag
    11161 atggtgcacc gtgtgctgtt gagggcgtta tcgagaatac tcaggattca ccattaagtg
    11221 gtttaactga acttgctttt actgatcgtc atttagtttg gttagccaaa gattggaata
    11281 gtgaaaatga tttttatacc ttaacaacaa attatttaca caatggcaat caaaaagcca
    11341 tgattaacgc attaaatgct ttctttaatc caatgaaagc tgacggaagg taggaaaata
    11401 aaatgaaaat aaaagaaatt ttaaatcgtt actgggcaat catttttgta gcaattccaa
    11461 ttattttgca gatgattttt ttctactttc caatggttca aggtgctttt tacagtttaa
    11521 ctaattggac tggtctgact tataattttg attttgtggg tttgaacaat tataagattc
    11581 ttttttctga tccgaagttt atcaaatcga ttggcttcac tttaattgtg acgctttgca
    11641 tgattgctgg acaaattggt tttggtattt tgattgcgcg ggcgttgaac gcaaatatta
    11701 aagggaaaac attctttaga gcatggttct tttttccagc ggtcttatcg ggcgtggttg
    11761 tttctttagt atttaaacaa atttttaact atggtttacc aaaaattggt agtatgttag
    11821 gcattgaatt tttacaaacc agtcttttag gaacaacatg gggagcgatt tttgcagtaa
    11881 tttttgtttt actttggcaa ggaattgcca tgccagtcat cattttctta gcgggttttc
    11941 aaagtattcc acaagaaatt attgaagctg ctagaatgga tggggcgaat gataagcaaa
    12001 ttttttggaa tattgaaatc acctacttat taccaagtat ttcaatggta tttattatgg
    12061 cattaaaagg tggcttaaca gcatttgatc aaatttttgc tttaactggt ggtggaccaa
    12121 gcgatgccac aacatctttg gggttacttg tttataatta tgcttttaaa aacaatcagt
    12181 ttggttatgc gaatgctatt gctttagtct tatttatctt aattgggatt gtttcactgg
    12241 cacaattaaa attatcgaaa aaatttgaaa tctaggaggt ttcccaatga aaacatcaaa
    12301 agaaaaaaat atttctaaat acttgctttt attattaggg ggcttcatga ttttagttcc
    12361 cttagtttca acgctattcg gttcatttaa aacaacaccc gatattatga ataatttctt
    12421 tcgttttcct aatccagcga ctttggaaaa tttccgaaga ttgttagcgg atggaattgg
    12481 tcattacttt tggaattcgg caatcatcac aatcattgca gtagttgctg tgacagcaat
    12541 tattcctatg gctgcttatt cactagcaag aaaaatgact cgtgggaaag catacggatt
    12601 gatgtattta tttttaattt taggaatttt tgttcctttc caagtaatta tgattccgat
    12661 taccgttatg atgagtaaaa ttggtttagc aaatatgtgg ggattaatct tactctatct
    12721 cacttatgcc gtaccacaga cactattttt atatgttggt tttattaaac taaatgtgcc
    12781 tatcagtctg gatgaggctg ctgaaattga tggggctagt cactttacaa cttatttcaa
    12841 aattgttttc ccaatgttaa aaccaatgca tgcgacgact attattctta atgctctatg
    12901 gttttggaat gatttcatgc taccattatt aattttaaat cgtgattcta atatgtggac
    12961 actaccattg tttcaatata attatacggg acaatatttt aacgactatg gtccaagttt
    13021 tgcttcctat acagtaggaa ttgtcacgat tacactagtt tatcttgctt tccaaagaag
    13081 tattatagct ggaatgagca atggagcagt gaaataacca ataaattatt ctcattataa
    13141 aggagttcat aaacttatga ttatatttga caatcacaca caaacatttc atttacaaaa
    13201 taaaaaaatt agctatctat taggaatcga agaaaataat tatttaacgc atctttattt
    13261 tggaaaaaag attacgcact attctggtgg gtatcgctac ccccgaacag atcgttcctt
    13321 ctcacccaac aatgcaaatg cgcaagatcg tttattatct ttagacacat taccattaga
    13381 atttccagga tatggacatg gtgattttag agaaccggcg cataatatta aattaggaaa
    13441 cggtagtcgt gtaaatgatt ttcgttatga tagctacgaa attcaaaaag gaaagcccag
    13501 cttagcggga ttgccagcct tatatacgga agatgatgat gaagctgaaa cgctgattat
    13561 tcaattgaaa gacgcgatta gtggattgac tttacagttg ttctacacaa tttatcaaaa
    13621 tcacagtgta attattcgtt ctacgcaatt agtaaataat agtgatgaaa cagtggagat
    13681 taaccgctta gctagtcaat caattgattt tccaaatcgt ccactcgagt taattcattt
    13741 aaatggtacc tgggcaagag aacgtcaaat gactagagaa aaaattcata cagggataaa
    13801 agtattagat agtaaacgcg gatcaagcag ccatcaacaa aatccgtttg ttgctttagt
    13861 agatgccaaa acagatgaat ttcaaggaga ggcatatgga tttaatttag tttatagcgg
    13921 taatcacgaa acggtgattc aaaaagatcc atttgaccaa acacgggtca ttatgggaat
    13981 taattcattt aattttggtt ggcaactaaa aactggcgac tcatttcaaa cgccagaagt
    14041 cgtgatggtt tatagtaatg aaggtctgaa tacgttatca cagacgtatc atgaattgta
    14101 taatcaccgt ttaattcgtg gaaactatcg tttaaaagaa cgtcccacgt taatcaacaa
    14161 ctgggaagca acgtattttg attttaacga agaaaaaatc atgaatattg tcgatgatgc
    14221 gaagaacttg ggcattgaaa tgtttgtttt agatgatggt tggtttggcg gtcgtgaaaa
    14281 tgacttcact tcattaggtg attgggttga aacagaaggc aaacttgctt gtggtttgga
    14341 aaatttagcc aagcaagtgc atgataacgg gatgaaattt gggatttggt ttgaaccgga
    14401 aatgatttct gaagatagtg aactatttcg agcacatcca gattgggcat tgggtattcc
    14461 aggtcgtgac cgctcaatta gtcgaaatca atatgttctt gatttttcaa gacaagatgt
    14521 tcgtgataat atctatcaac aaatgacggc gatttttgat cgtgtaccga ttgattatgt
    14581 gaagtgggat atgaatcgaa atatgacaga agtctattct gtcttgttgg actcaacgat
    14641 gcaaggtgaa gtggctcatc gttatatgtt agggttatat gaatttttag aaaagttaac
    14701 aacgaattat ccaactattt tatttgaaag ttgttcgggt ggaggcggtc gttttgatgc
    14761 aggtttcttg tattacatgc cacaaacttg gacgagcgac aataccgatg ccgtggctcg
    14821 tctgaaaatt caatatggaa ccagcttagt gtatccaatt tcaagtatgg gtgcgcatgt
    14881 ttcagcagtc ccaaatcatc aaacaggaag agaaacgagt ttaactattc gtggcaatgc
    14941 tgcgatggct ggtgtttttg gttatgagct agatctaacg caattaactt ctgaagaaaa
    15001 agcagaaatt aaagaacaag ttgcctttta taaaaagcat cgccaacttt tgcaatatgg
    15061 aactttttat cgcttggaaa gtccgtttga aggcaatgat actgcatgga tgtttgtctc
    15121 aaaagaccaa acagaagcca tggtctttta tttccgagta ttagcagaag ctgcgcatcc
    15181 attagtcaca ttgaaattag caggattagc agaagaagca acttatcaaa tggaagatgt
    15241 aacatttgct ggcgatgaat tgatgaatct cggtttttat attgactcag aactacatgg
    15301 tgattatgcg acacaacgct tttatttgaa acagatatag tagcttgaat ttacaatata
    15361 agtgcaacac caaaagagac tcattccaaa cgaagaaaat atgttttatt tacacgcatt
    15421 tactaaaaag cagcctgatt tgaattggga aaatgaagaa gtacgtgaag aaatctatgc
    15481 aatgattaac tattggttag ataaaggact aggcggattt agaatcgatg cgatattaaa
    15541 catcaaaaaa aaactagagt atggcatctt tgaatctgat ggagaagatg ggttagcctt
    15601 cattgggccc tggattctta atcagtcggg cattgaagtg tggttaaaag aaatgagaga
    15661 gcggacattt aaaccacata atagtatgac ggtagcagaa gccgatgttc ctaatgaacg
    15721 tttagatgaa tatattggtg aagatggcta ttattcgatg gtttttgatt ttagttatac
    15781 ggatattgat gtacctgaaa ctggtgaatg gtttaaagat agccaatgga catggacgga
    15841 tatgcgtacc aatattttta cgaatcaatt agttacgcaa gataaaggtt ggggcgcctt
    15901 atatttagaa aaccacgatc aaccacgttc aattaataaa tatattccag aagaatggat
    15961 caatgattat agtaaaaaaa tgttggcgac gctttttatg ttactaagag gtaccccttt
    16021 tatttatcaa ggacaagaac taggaatgac caacatagaa atggcatcat tagaagattt
    16081 tgatgatgta gctacgcatt ctcagtataa acgggcatta gaatatggat tggcaccaga
    16141 gcaagcattg aaagctgtca ataaacgaag tcgggataat tctcgcacac ctatgcaatg
    16201 gaatacgcaa aaaaatgcca gattctcaac ttctgataat gtatggttaa aagtaaatcc
    16261 taattatcca gaactcaatg cagaagctca gttaaccaat caacagtcgg tattcaatta
    16321 ttatcatcaa ttaattaaat taagaaaaaa tagtgtatac aaagacgtgc taatctacgg
    16381 acaatttcta ccagtgaaag ataaaaatga acatattttg ctttatgaac gccaattaga
    16441 agataaaatc gtattagtta tcttaaacgt tacagcaaca gaacaagaat attatgtaga
    16501 tgaacagtac caacaagtat taatcaataa ttatgaagat gttttattag atcaaagtcg
    16561 aaaattgcgt ttacgaccgt ttgaaagtat tgtattagct aattatggag gacaattatg
    16621 aaaattaaaa acgaagcaat gctcatcacc tatgcagata gtttaggaaa aaacatgggc
    16681 gaattaaaac aagtcttaga caaacattta caaggtgtta ttggtggtgt acatctgttg
    16741 cctttttttc catcgacagg tgatcgaggc tttgcgccga gtgattatac gacagttgat
    16801 ccagctcttg gaacatggga agatgtggaa gcattaggtg aagattatta tttgatgttt
    16861 gattttatga ttaatcacat ttctcgtgaa tcaaaatttt tccaagattt caaaaaaaac
    16921 catgagcagt caccatacaa agaaatgttt atccgtattc atgaattttt cccagaaaat
    16981 cgaccaacac aagaagatat tgatttaatc tacaagcgta aagacaaagc gccgtttcaa
    17041 acggtagaat ttgcggatgg tacgacagaa gaggtttgga atacctttgg ggaagaacaa
    17101 attgatttag atgtgaccaa agaagttgtg aaagaattta ttcgagaaac catcaaagat
    17161 atggcgaacc atggctgttc tttaattcga ttagatgcgt ttgcttatgc gattaaaaaa
    17221 ttagatacca atgatttctt cgtggaacca gatatttggg aacttttaga cgaagtgcgt
    17281 caagaagcag cgaaatatga cgtcgaatta ttaccagaaa tccatgagca ttattccatt
    17341 caaatgaaaa ttgccaatca tgattattat atctatgact tcgccttgcc aatggtcact
    17401 ctgtattcac tttatagtgg aaaatcagaa cgtttagcca attggttgaa aatgagtccg
    17461 atgaagcagt ttacaacatt ggatacgcat gatggaattg gtgttgtgga cgctcgtgat
    17521 ttattaacag atgaagaatt ggattacacc tctgaggaat tgtataaagt cggggcgaat
    17581 gtgaagaagg tctattcaag tgccaattac aataatttag atatttatca aattaacagc
    17641 acctattata gtgcattagg cgataatgac cgtagctatc tgttagcacg tgcgattcaa
    17701 gtatttgcgc cagggattcc acaaatttat tacgttgggt tattagcagg aaaaaatgat
    17761 attgaactgt tggaagcaac gaaagaaggg cgtaatatta atcgtcatta ctatgattta
    17821 gccgaaattg aagaagaagt gcaacggcca gtgattcaac aattatttgc acttttaaaa
    17881 tttagaaatg aatctgcggc gtttgatttg gatggaacga ttgaagtagc aacgccatca
    17941 gaatcagaaa tagtgattac acgtaaaaat aaaaacggta gtgatagtgc aacattaacg
    18001 gctaacttgg atacgaaaga atttatcatt gagtctaaag atataaaaat tttcactagt
    18061 gttgcattaa aattttaacc tattccatag aacaaaaaat cctttatact agcacttgga
    18121 tgacttatcc aagaccaata aaaaggactc atacatggat aagtataaaa caaatacagc
    18181 atttaataaa tggttttctg ccataaaatt aggaaaactt tctgtcaact cccaaaaatg
    18241 tatttttgat tacaattcgt atcgtaaaaa actgtctttt gaagctgtgc tcaagctctt
    18301 tctctacgca gttaatgaag aaaaagaaag tttgcgtgat ttgagtacag ctcttatcga
    18361 ccgatcgctc caactagaaa ccgatgtgtc atcgattagt catacccaat tgtctcgagc
    18421 atttaatgca cttgaaccta aagtgttaga agaaattttt caacaactgt tagaaaaagt
    18481 tcaacatcaa atcaaaccaa ccaagcgaaa tcatctgtat ctgattgatt ccagtacgtt
    18541 ttcgcttagt ttaaaacgtc ataagtgggc gactttccgt caaacaaaat caggtgtcaa
    18601 gttacactta aactattgtt atatggacga tactacgatg tatccaactg attttacctt
    18661 aacaaatgcc catgaacatg atctcaatca actaccagta ttggtgaatc aaccagaggc
    18721 aacttacgtg tttgatcgtg ggtattagga ctttgaaaag atggatcaga tgcattggga
    18781 tggctatttc ttcgtcactc ggattaaaaa aaacaccaaa gttcacgtga tagatactct
    18841 ggaaacatcg ccagaaacag aaattctacg agacgagctg gttcgtttag gatctaaaac
    18901 ctatctaacc gccaatttcc gactagtcac tgttcaagat aagaacggac gtgtgtttca
    18961 atttatcacc aatcgaatgg acgtctcttc aaaagaaatc tctgatatgt atcacgctcg
    19021 ttggcaaatc gaattgtttt tcaagcacat caaacaacac atgaccatta aaaccttctt
    19081 ttctcaaagt gaaaaaggcg tgcagaatca attgatttta acgatgattt ccgctctcct
    19141 cacgtttctc atcaaattgg agacgaaaac ggaaaaatca gtgttccaaa tcaaacgatt
    19201 ctttcgttat ttactgtttc agccctttga atgttgtcta gaaaaattaa ttcccacatg
    19261 agaaaaaaga aatttggctc gttacttttg gttttcagtt gaataacaga taaaaaattt
    19321 tctggaaaaa gtcatcctct gcgtcagcac agtttttttt gacttatttt cagaggaaat
    19381 agttttcaga atattctaaa atgttttatg caacactagt gatatttttt agaattagat
    19441 ttctaatgtg cattttttga gtattaagga gcaagtatac aaagatttat aaagctgatt
    19501 aaatttgaat aaaaatatgc aggaacgcta ctatttttag tagtgttctt ttttatattt
    19561 gaataaaaaa tgtggtggtt aaatggtggt tataacaaaa aatgaattaa tgaaattagg
    19621 ttatgggaag tatcagtctg agagaatcat acgacaggca aaagcaatta tggtttcaaa
    19681 aagctatacc tattacgaaa atagaagatt aggactagta cctgttgaat ctgtagaaga
    19741 agttttaggt atgaagatag aggtagagga ggattttcag aatcatggtt aattttaaaa
    19801 aagataaaaa tgggaaatat cattttactg ctagtttggg attcgacaaa cgaacaggga
    19861 aaagagtaca aaaaaacgta gaggggttaa aacagttaaa gaagaaaaaa agcgtatacc
    19921 gaacttgttt ctaattatgg aaaagaagca gaaacttctc aaagtactat gttgtttgag
    19981 gagttttttt ataaattatt cctaccttat tatcaaggga aagtagaaga aagaacatat
    20041 aaaaatcggt tatctacaat caaaaaaact tttcttattt ctttaaaatg gagttaagag
    20101 aaatacgtcc aattgacatt caaaagtggc aaaataattt attgagcaaa tataaaaatt
    20161 cttatgtaag aaacatgtat gggatttttc agatgtcatt agatcgagca gttgttttgg
    20221 gactacttga aaaaaatcct gcaaaagtta ttggaaatgt gaagaaaagg aaaactacag
    20281 ttgatttttg gacaaaagaa gaatttacaa aagttatttc aactttttat attgatgatt
    20341 tgtaccaaca ttttggtttt atttgtatgt ggttactttt tatgaccgtt atgcgtttgg
    20401 gagaagccac tgcattaact tggaaagata ttgattttga aaataaaaac ttgcgtgtga
    20461 ataaaacact ctattataag agagccaaaa cctatgagtt tgtagaaccc gaaacggctt
    20521 ctagtattcg tacaatcgaa ctagacgaca atacaattac ctttttaaaa gcttggaagg
    20581 agcgtcaagt ttcaatagga ggcatgtcgt ttgtactttc ttataatgga acacctactc
    20641 agaaatatac aatccgtcat attatgagaa gacatgcaac gatcgttggt gttcatataa
    20701 tccgcataca cggattaagg catagtcatg cttctttgct tatatcacaa taaaaagatg
    20761 cacttcgaat taaagaaagg ttaaaacatg ctgatgtaca aacgacttta ggaacttatg
    20821 gtcatttgta tctaaattca ttgagagaga taacaaatga tcccacagat tatatccaaa
    20881 ctaatttttc taaaatcaaa ccgacaccta tttacgataa taaaaaataa atgataaaca
    20941 aaagtaggat cacaccaaaa gaaacacact agaaaaacta taatacaggc ttttctagcg
    21001 tgttgtttct tatttccgcc tatttataca ttatactcat aaagcattta atgcatcgat
    21061 cgtagccgca ttgtctatat gggtatataa agttgttgta ttggtacttg aatgtcccaa
    21121 ttgttgggca gttaatacca atgaatttgt ctgttggtag agtctagtag ctaatgtatg
    21181 acgcagtttg tgagggctga cttgaattct aaagcctttt gaatattttg ctaccatccg
    21241 ttcgattgca ataccggata tacgcttggc ttctccttgg taaattgcta gaaataaagc
    21301 tttttcgttt tctgttactt tatatcgttc tttccgaata gctagataat tagctaaata
    21361 ttccattgcg aatgctgcta tattgacagc atctctttta tttccttttc tagttactac
    21421 cacattattc ttatctaaag ataggtcatc taaattaata tttataacct cggagagtct
    21481 caatccagat cctaaaatca gtgcattgat cgccaggtct ctttctacat ctcgttgata
    21541 atagatcaaa gctcgtttac tcaaagtatt tttatattca ttggcaatat aatctagata
    21601 attttgtgta tccttatcta gaaataattt tttctcaatg gcattggcac gtgcagctaa
    21661 cgtagtttta tctgtgatgg ttgctacttg atccatagga ttattgtcta agtatggttt
    21721 tcctagagga ttattggaac ttttcgaaag atatttaaat agaaccttta atgccgtgat
    21781 atttctattg atagtagaag tagatagacc taagttatta ttatctttga gcttagtttt
    21841 tgttgtctct ttttttacat tttcttttgt gcgacgtttc aaatgagatt tgtacaagtt
    21901 taggtcatag gcagttaact gtcttatcga ggtgatagaa gtatcaggaa cgatagactc
    21961 actcagaagc cattcataaa agagcttata attttttaga tattctaaag tggttcgagg
    22021 tgattgatct ttatcctcac gatcttgaac aaaatctttt atataatcgg gcatatgaag
    22081 tatgattgta tctatttttt gatatatttc tgtatttttc atgtcattca tcctttcaat
    22141 aagtagggga gaagagtatc tctataattg gactatacca attatagaat gttttgtcaa
    22201 ttggtatgct ctaaaattta ttaatttgtt tcaaaaaaag catataaaca ggtatacgtt
    22261 taataattta aaataaatgg tatacataaa tagggaagga aatagaaggg agcaatctaa
    22321 attttttgaa ttttcttaat taattatata gaaagaacaa tctttctata gagttagaca
    22381 tgagagggac tatgcttgat ttaaaatcaa caagcgtata tactgtatat acaaagaaaa
    22441 aggagggaga agactatggc aattaaagaa agaaaattta gggaagtggg gaactcagta
    22501 gtattaacta tttcaaagga gtttcttaaa gaaaatgggt taaatcccgg agatacggtt
    22561 tttttagatg aagaaaaact tcgagcggca attacaaaaa aacaagcctt atctgatgaa
    22621 attgatttag caatatctca agcattaact gaatatgata ctacttttag agagcttgtc
    22681 gataaatgag tattcattac ctagaagcca aagatattat aaaaatgaat gtaatccaaa
    22741 taaaaaaata ctctcctaat gaaccccttg gtgtaaagga tgcgaatgct ttagaaatgt
    22801 gtgtagggca acttcgagcc tcagcttttg gtgaagaggt ttatccttcg atttatgaaa
    22861 aagcagctat cctacttata caattgataa agaaacatcc ttttcacaat gcaaataaac
    22921 gtactgcttt cttagctact tttgtctttt tgaaaatcaa tggacagctt ttagctattg
    22981 atcaaaaaga tgctgtaaat ttagcggtat atattgctac gtatgataag gatttcgatc
    23041 aactaaaata tgaagtgatc aacactataa agacacatac gcagcccatt taaggagtta
    23101 ctgtatgtgt ctttataggg ttattagctg ctatagcgaa ataattgaag taaattacaa
    23161 atagctataa ttcaaatcca actatataga aaacgtaaat tttctatata gttggattaa
    23221 gataacgact atttatttta tcatattttt actataccct atacttttac ttggtatatt
    23281 tatgaataat cgcaaattta cccctatact actagtttgt aagttagatt aataaaaata
    23341 ctaaagttat ttgttctttt attaaataca cctacaactt ataaattgat ggtatttaga
    23401 taacacgaaa gaataaaaat aacagctgat agtaaagttt gttgacgatt gctactcaat
    23461 cgaaagtgag ttagtgcgta tgaacttgct tctgaaaact tatgaaatga aagacctttt
    23521 acatcttgca gaaatcggta ctgggattaa actaagcaat aaaaactaat gttcccctca
    23581 ttctttgaac ttctactatt tgatttattt attctgataa atgacgattt caccttcagg
    23641 atcttcaaat cccgtataga atttatttaa gatctcttca gtatcagtaa taggataggc
    23701 gataggccac ataccctttg atggttagaa taaactgctc aaaatctgta tcgtatgttg
    23761 gctttagcat attaacaaag gctatctcca aattataaac cataagcgtt gaatcgttct
    23821 taacaatttc aattcttcct aacccaaagt cagaagatat aattaccgga cttgccttac
    23881 ccttcatgtg ggttgttgag attccatatt taaagggaat tgtcggtact atcggcggtc
    23941 tgaaaaacat cccaaaaata aaaaaatctt tcccctgcta gtctcctaag tccctttgta
    24001 gcggatatgg caccataatc tcattttatc gaagtgagtc atggtttatc tgttggccaa
    24061 ccaaaattga tacaatctaa cgatgcaccc tctacggcat tctaggggag tgcacttgca
    24121 ttcacccaat aaaaaccggc agttagcaaa gaacttatgt agtcgtatgt tcttactgtt
    24181 aacatacaag aggtctcctt tccacaattg cttagcagta aacgggaatt tacaaagcta
    24241 atctcttcca ttttcttatc tttgttctaa aagtcccaaa tggagcaact gtatttacat
    24301 gaataaattt atatacttcc catacagccg ttttggttgc ctcgtctgcc cattttcttt
    24361 tgtgtgcagt gaatagatct tcatcactca ttgaatcaat catcatgtta atatcatcaa
    24421 tattacgatt gagaatttcc tctaattctt tcaatggtag atgtgcatag gtatttgtaa
    24481 accacttata taactctcct aactgattcc atttaaattg atcagaaggc gttttaacag
    24541 ataatccatt tctttcatca ttttcccatt ttagaattaa attcgtccaa ccaacttgat
    24601 atgctaaatt ttcagcaggt gttctatcca cttcctcaac tcttttatcc ttcaattctt
    24661 caggaatagt ttcaaattct aaaatatatt tagtataagt cctatttatt tcttttttta
    24721 attcttcttt atttttatat gttctcatta tattacctcc ataaattcct aattgtctcc
    24781 tttaattcat ctataatttc atccacgata ttttaatccg ttctacaatg catatctgat
    24841 atgcattgac tgtctaactc atatgtgaat gatttataaa atatcccaac gtctcatcta
    24901 aagaaatgtt gatcgtcagc aaggagctaa aaaatacgaa tatatttctt ttgtagtaaa
    24961 atcggactgg tttgcatatc attttcttcg taaatatcaa aatcatgtta actactatca
    25021 attactaata aaaactacca ctttaagcgt taagaactgt taagtttagt taagtgagga
    25081 tagttcacaa aatccattgt taaacttcac gaaagtggta aaactcaaaa tgctttatat
    25141 aggattacag catcgctctt tcttcgattg cgcgttgggt aaaaaatatt atgaagtaaa
    25201 aattgacgat gacacgttcc ttacggctca tcagatgaaa cagctacaaa aacgaaatga
    25261 tcaactcgaa gaaaatttaa ttctaaaaaa gcgattgcca tattcacgcc tcgctcgacg
    25321 aactattaaa ggtcaagatt tacctgaaga tcatcctttt taggaaattg aagcttttta
    25381 tgttgcacaa tgcatttata atactacaat gatgttttca ccagatattc ttctagggtg
    25441 cggtgtaatg aaacagatac acttgcaaaa gaaaattcac cgcgaattta ctaaaattat
    25501 gaataattat gttaatcttc cgaaattttt ttccaaaatt aagggatcaa gctggtgtta
    25561 tagattgttt aaggttggca aaaaaataac ttctttttaa acaattatag aaaaccgata
    25621 ctctgttaag ttggttgaca cggtgtcgat ttttatgact ttcttattct gaaacttcac
    25681 taacggacag tacctaaaaa gtctatagta tggacgttta cgagtactat tttatcaaaa
    25741 atgctgctca tttcaactta tatgttttag tgaactaccc ccactta
//
LOCUS       DACDXO010000034        25502 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E23605_2017
            SAMN12639989-rid9881463.denovo.034, whole genome shotgun sequence.
ACCESSION   DACDXO010000034 DACDXO010000000
VERSION     DACDXO010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639989
            Sequence Read Archive: SRR10023389
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741914.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:42:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,972
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 33 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 17 of 73
            Pseudo Genes (multiple problems)  :: 10 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25502
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639989-rid9881463.denovo.034"
                     /isolate="E23605_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            133..3660
                     /locus_tag="IWM35_001620"
     CDS             133..3660
                     /locus_tag="IWM35_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAP9919476.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3657..7379
                     /gene="addA"
                     /locus_tag="IWM35_001621"
     CDS             3657..7379
                     /gene="addA"
                     /locus_tag="IWM35_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HAP9919477.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7448..7789
                     /locus_tag="IWM35_001622"
     CDS             7448..7789
                     /locus_tag="IWM35_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9919478.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8098..9141
                     /gene="pheS"
                     /locus_tag="IWM35_001623"
     CDS             8098..9141
                     /gene="pheS"
                     /locus_tag="IWM35_001623"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HAP9919479.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIERKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9146..11566
                     /locus_tag="IWM35_001624"
     CDS             9146..11566
                     /locus_tag="IWM35_001624"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HAP9919480.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11736..12746)
                     /gene="trpS"
                     /locus_tag="IWM35_001625"
     CDS             complement(11736..12746)
                     /gene="trpS"
                     /locus_tag="IWM35_001625"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HAP9919481.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
     gene            13000..13737
                     /locus_tag="IWM35_001626"
     CDS             13000..13737
                     /locus_tag="IWM35_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAP9919482.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            13751..14566
                     /locus_tag="IWM35_001627"
     CDS             13751..14566
                     /locus_tag="IWM35_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HAP9919483.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            14579..15226
                     /locus_tag="IWM35_001628"
     CDS             14579..15226
                     /locus_tag="IWM35_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAP9919484.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            15242..15898
                     /locus_tag="IWM35_001629"
     CDS             15242..15898
                     /locus_tag="IWM35_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAP9919485.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            16060..16881
                     /gene="racE"
                     /locus_tag="IWM35_001630"
     CDS             16060..16881
                     /gene="racE"
                     /locus_tag="IWM35_001630"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HAP9919486.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            16884..18239
                     /gene="rph"
                     /locus_tag="IWM35_001631"
     CDS             16884..18239
                     /gene="rph"
                     /locus_tag="IWM35_001631"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HAP9919487.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            18240..18758
                     /locus_tag="IWM35_001632"
     CDS             18240..18758
                     /locus_tag="IWM35_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HAP9919488.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            18865..19365
                     /locus_tag="IWM35_001633"
     CDS             18865..19365
                     /locus_tag="IWM35_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAP9919489.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
     gene            complement(19645..20565)
                     /locus_tag="IWM35_001634"
     CDS             complement(19645..20565)
                     /locus_tag="IWM35_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HAP9919490.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
     gene            20730..21743
                     /locus_tag="IWM35_001635"
     CDS             20730..21743
                     /locus_tag="IWM35_001635"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HAP9919491.1"
                     /translation="MQIDWNNLGFSYIKTPWRFIAKWKDGQWEEGELTEDNYLTIHEG
                     SPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLMPMVPEKMFVDAVK
                     QVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVFCTPVGAYFKGGLK
                     PTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCVYLDPATHTKIEEV
                     GAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIEGDIFIDDLDQFAE
                     AGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGIQFGDIEAPEGWIE
                     VVE"
     gene            complement(21853..22608)
                     /gene="fabI"
                     /locus_tag="IWM35_001636"
     CDS             complement(21853..22608)
                     /gene="fabI"
                     /locus_tag="IWM35_001636"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HAP9919492.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
     gene            22828..23268
                     /gene="fabZ"
                     /locus_tag="IWM35_001637"
     CDS             22828..23268
                     /gene="fabZ"
                     /locus_tag="IWM35_001637"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HAP9919493.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
     gene            complement(23383..24144)
                     /locus_tag="IWM35_001638"
     CDS             complement(23383..24144)
                     /locus_tag="IWM35_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP9919494.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            24333..25427
                     /locus_tag="IWM35_001639"
     CDS             24333..25427
                     /locus_tag="IWM35_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAP9919495.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
BASE COUNT         8448 a         4424 c         5384 g         7246 t
ORIGIN      
        1 tttttgcgta tacttaaaaa agtagaaata tcagaacctt aaaaggatat aatttgaaga
       61 atatagaaca aatgttcagt attttttgat ataataaagg agcaccgtat catgtgttga
      121 aggaggaaaa gcatgtcttt acaatttatt cttggaaacg ggaaaaaaga tcaccgtcaa
      181 gtcttgatcg acgaagcatc cgcttggctc gaccaggatg caagtaacca agtatttttc
      241 cttgttccca actacagcaa attcgaacag gaacaagaaa ttttagcgga gatgcgaaga
      301 agaagcggaa aaaaaacttt cagcacaaca aacgttcaag tatttagttt ttatcgtctg
      361 gcttggtatt tgctgcaaca gacaactctt cttacaggaa atgaattatc agaatcagga
      421 tctgcaatga tcttgcgaca gattttagaa aaaaatgagg aagaattaac aatctttcgc
      481 ggagaaatca ataaaagagg gtttattcgt caattacaag aactctacag tgaattgcgt
      541 acaggaaata ttcaaccaga agacctgatg cttttatttg gtgattcacc taatgcagaa
      601 gaccagcaac ttaaaatgaa agacttgaag ctgatttttt cagcttatga cagcgaattg
      661 actcaacgtg cactaacaaa tgaagatgca ctatctatac tagcaaatta tatgcagaca
      721 caagatttca acaacgtcaa atttattgtc agtggttttt ctcgaatcaa tggtcaagag
      781 tatcaattac tgcaggcaat gatggaaaag gggcaattgg ttgtcgattt attgctggat
      841 cgtccttata cagcggatct tccagaagta cttacgctct ttcatgagac tggaaagctt
      901 tatttccgtc tttttcaaag tgcaagagaa cggaaagtcc ctatattagt ggaccgattt
      961 gcaccagagc agactcgtcc gacagagctt caactgctgc aaacaatgtg ggaagagaca
     1021 tctagccaaa agaaagcggc agtacacaaa atagggggac aagagcatct ccatttgtgg
     1081 gaagctgaaa atccaactga agaagtccga caattagctg ttgaaatcag acgactagtc
     1141 agtgaagaaa actaccgtta tcgtgatatc caagtgctga caaaaaatat gtctttatat
     1201 ggaaatatcc ttcggccgat ctttcaagaa atggcaattc ctttttatat cgatgaagaa
     1261 caaatgatgg aaaatcatcc gctgatcgaa tggatcaaca gtctgttcgc tcttgaccgc
     1321 tattcttatc ggctaagtga tattatgcgt tttttcaaaa ctgaattatt tctgtcgacg
     1381 gaagcagaaa ccgaacttga tgaatggaaa caagagcgag atgaattcag gaaaaaactg
     1441 gacatcactg aaaatgtagc acttgcttac aattatcaag gaacttattg gactcgtgaa
     1501 aaagactggc agtttgtggc gtatgatttt gaagcagaac agattgaaga cgtacagtat
     1561 ttggaagaac agtcaaatac gattagacga ttatttcaga aagctgtacc agtatttttg
     1621 aaaaaaataa agaaaagcaa aacaggacaa gaggccgcaa atgtttttta tcaatttttg
     1681 atccaaagtg gcgtacagca acaattattg ttttggcgta atcaggaagt agacagagga
     1741 aacttagaag cggcaagaaa ccatgagcag acatggggag cgctgattga tctgttggat
     1801 gaatatgtag taatttatgg tgaatcggct tttgactggt cactgttcca agaaatcatt
     1861 acaagtggtc tagaaaatct aagttatggt aaaatcccta ccgcaatcga ccaagtgcga
     1921 atcaatcgtt tggaattagt tcgaccaaat caagctgccg tcacttttgc attagggttg
     1981 aatgatcaag tgtttccaga ccgaaaagag acaaaaggat tgttgtccag tgaagaacga
     2041 gaagtattaa atcaggcctt gccagatgga cagtttttat tcgacccatc aaaggaaagt
     2101 atctcttttg agcctttcca agcctatctt gtttttcttt ccggaacaga tcgattatat
     2161 cttagttatg cacaaagcta tgacacagat ggttcgctga agatgtctcc ttatttgcgt
     2221 agaatcacag aatatttatc tgttcccgtc gagagaaaag agcatttgac gatcgaaagc
     2281 gaccctaatt gttatgtagg gacttacaga agcggaatca atacagtcaa taggatttat
     2341 cgattagctt tagatgaaaa aaaacctgta ccaaagcact ggcaaaagct caaagaatta
     2401 gtactgaatt cgtcatggcg aaattttgct ttacagattt ttgaaagtca ggaacattta
     2461 aatgttcctg tttcgatgcc agaaaaaatg gcggaagagc tttatggaaa agagatttat
     2521 acttcaattt cacgtatgga gaatttttat aattgtgaat acaaatactt catgcaattt
     2581 ggtttgaaat tgaaagaacg aactgtttat ggtctaactc cagcagcaac tggtgatttt
     2641 tatcatgaat cattggatcg tttcttcaag ctactgtttt caaaccaatt atccttagtg
     2701 aatatgtccg atgcgcagcg tagagacttt actgaaaaag tgctgcagga agtattcgga
     2761 gaacttcggt ttgagatatt ggatagttct gcaagaatga actatatccg ctaccagtta
     2821 ggacagacga ttcaaaaagt gacatgggct ttgcaaaaac agagcaaaaa tacaggcatg
     2881 catcctcttc agacagagat tctatttggt cagattgctg gagcaaaagg tatcacaggt
     2941 ctggaattac cgttgagtaa tggtggaaaa ctccatgttc gagggaaaat cgatcgaatc
     3001 gatgtagctt ctgaacaaga agatacttgg ctaagcgtcg ttgattacaa atctagcgga
     3061 cgttcattcg atgtcacaga agcttattat ggcatggcta tgcaattatt gacgtatctg
     3121 gacgtagcat tgatggatgc ggtacgtttg actggaaagg cggctgttaa accagcagga
     3181 tcatattatt tacatgttca taatcctgta ttgacggaga cagatgatgt agagaagaat
     3241 acattgaaaa aatttagtta tgatggcgtt tttgtcgatg atcccgctct tttagaagta
     3301 ctagatcatt cactggaagc aaaagaacaa tcactgatct atccagtgaa aaaaaatgcg
     3361 aaagatgaat accaaaaagg tagtttcagt aaagacaaat ttttcacgga agaagaactg
     3421 caactattca tggcgcataa tcgggaaaat atgcggttag ctggagatgc gattacttcc
     3481 ggagaaatca aactaaatcc agcttataaa gataaacagc ggattgcctg cgaattttgt
     3541 ccattcagaa gcatctgtac gtttgatgtg atgctgaaag aaaataatta ccaccgtctg
     3601 gagaaaatag ataaggaaga agcactgcgt agaatattga aaaggagtga agaagagtga
     3661 atcaaattcc tttaaaaccg caaaatgaac gattcacaga tgatcagtgg caagcgattt
     3721 ttgatcaagg agataatcta ttagtctctg cgtctgccgg atcaggtaaa actacagtgc
     3781 ttgttcgaag agtgattgaa aaactaaaaa tgggttttga tatcgatgaa ttattgattg
     3841 tgacatttac tgaagctgcc gcaagagaaa tgaaagaaag aattcaagag gcattacaag
     3901 aatccgtcaa tagcgaaagt gaccctgtac gtagacagca ttttactaag cagttagttt
     3961 tattgcccac agcgaatatc tcaaccttac atgctttttg cctgacagtt attcgacgtt
     4021 attattactt aattgatatt gatccagtct ttcgcatgtt aacagatgaa acagaaacga
     4081 tcttgatgaa ggaggatgtg tgggacgagc tgagagaagc actttatgca gaaaatgatg
     4141 aacgattttt ccaattaaca atgaattttt ctaatgatcg ttcagatgac ggcttgacga
     4201 atctggtgtt ttctctttat gagtttgccc gtgccaaccc tgacccccaa aaatggctag
     4261 aacagttaag cgataattat cgtttgcctg aggggctagc gaaatctcga ttatatcaaa
     4321 aacaaatccg tcctttagtt ttggcagata tataccaatg tgtacagtta tatgaacaga
     4381 tgactcagtt agcacaaggt gaaggattag aaaaaatgca tgagcaagta gctggagaac
     4441 aacagcagat caagaatatc tatgaagcgt tctcgcaaga tcggttagaa gaagcttatg
     4501 ctggtttgga acaactaact ttttcaactt ttaagagcag tcggaaagca gaattgaaag
     4561 aaatttccaa tgaagtcaag ggcatgaggg ataaagcaaa aaaactgatt cagcagataa
     4621 gtaaaagtta ttttcctgtt tccccaagcc aaatggaaga attaacggac aaagcactac
     4681 cgttagtcga agaaatgaca aaagtcacgc aaagcttcat ggacggtttc agtatgcgta
     4741 aacgggaaaa aggtgtgctg gatttcaatg atctggaaca tttagcgttg cagattctca
     4801 cagaaaaaac gaaagatgta tggctgccaa gtgaagcttc aaaacattat cgaaaaaaat
     4861 tcaaagaagt aatggtagat gaatatcaag acgtgaacca attacaagaa gccattttat
     4921 actggctaag agaacccgat gatacaaaag gaaatatgtt catggttggg gatgtcaaac
     4981 aatccatcta ttctttccgt ttagcagatc ctagtttatt tatcgggaaa tacgagaatt
     5041 tctcaaagaa agaaggcgga cgtcgaatcg ttttggctga gaacttccgc tctagaaaag
     5101 aagtcctttc ttttacgaat cttatttttg aacaactaat ggatccagca gtgggtcaaa
     5161 tcaattatga cgaagcagcc aaactgatcc aaggattttc agactttcca gagaatgaac
     5221 agtttgagcc ggaaatcatg atctatgaaa aagaacagga agaatcagag attgagattc
     5281 cgacggatga tatactggaa gacaaaacag aaggcgaact ttttatgaca ggactaaaaa
     5341 ttcgtcaatt aattgattcc tcctttatga tttatgataa aaaatcaaaa aaaagtcgtc
     5401 cgatagagta caaagacatt gttctcttga cacctacaaa gaagaacaac ttaacgattt
     5461 tagagatttt caaaacattg gatattccat tagagatgaa tgatgctcaa aactattttc
     5521 aagcgacaga aattcgcacg atgatctcgc tgctacaatt aatcgataat ccctatcaag
     5581 atattccgct tgcggctgta ttgcgctcac cgatcgtggg tctgattgaa ccagaattag
     5641 ccagcattcg attagctaac cgtgcacata cttactatga tgctgtgctt gcttatcaag
     5701 catcaaacga agatgaatta gcagctaagc tggaacattt tggcaagcaa ctagagcatt
     5761 ggcgagaact tgctcgccgt tcttcgatca ctgacttgtt atgggatatc tattatgaga
     5821 cgggatatct agagtatgtg gtcggtctgc cagcaggggc acagagacaa gccaatcttt
     5881 atgcattggt tgatcgtgcc aaagcttacg agcaaagttc tttcagagga ttataccagt
     5941 tcgtgcgttt cattgaaaaa atgcaagaaa aagataaaga cttagcagaa ccagtcattt
     6001 caatagaaga taatgctgtt cgcgttatga cgatccatgc aagtaaaggc ttggaattcc
     6061 cagttgtatt tttgttagat atgacaaagg agttcaactt acaagacttg cgcaatcggt
     6121 atgcttttga agaaaaactt ggagcaggaa ttcgctatat ggatcctgaa acacgagtgt
     6181 tatatgatac gttaccgttt caagccatca aattggcaaa gcaaaacaaa ttgttgtcgg
     6241 aagaaatgcg taaattgtat gtaggattga ctcgggcaga acaaaagctt ttcattgtcg
     6301 gttcctataa aaataaagag caaatgatcc aaacgtggtc tgaagcagct gatcatgagg
     6361 agttagtttt tgatcctgcg ctacgtttga agggcagaag cagcttgatg aattggattg
     6421 gttacggatt gatccgtcat cctgaaatgc agaaatactt agaggaagaa atttcgactt
     6481 ctcttcttca acattcaaat gcacagtttt ctatttcatg gatgaatcag caatcgatca
     6541 ttgaacaaag acagttgctt gctgaaaaag aactggttaa cttagatcag cagatgaaag
     6601 aagatgaaac tttattagca gattcgttac aaaaacgtct ggcatatgaa tatccttatc
     6661 aagcttcttc acaaaccaca agttatcaat ctgtatctga aatcaaacga ttattcgaag
     6721 atccagatga tacacaagaa tcacgtttga ctctggaaag cagtcagaac aaagcagcat
     6781 cccgtcaatt tcgttatacg caagaacaac tagcagaacc aaaatttttg caaaaagatc
     6841 ggcaagtgtc agcagctgct gttggaactg ctacgcatgc tttgttacaa ttactgccat
     6901 tagagatgcc aactacagag agtatccatc aaaaattaca ggaattggta aggaaacggt
     6961 tagtagacga aaaagtcgcg aaaaaagtag atgtttcttc tattatatgg ttctttcaaa
     7021 cagaacttgg tcaacagctg attgccaaca aagaaaacgt aaaacgagaa caaccttttt
     7081 ccatgctgct tcctgctgac gaagtatttc aagactatcc aaatcaagaa gatgaactat
     7141 taatccacgg aatcgtcgat ggatacctag aagaaaaaga tcatctcaat atctatgatt
     7201 ttaaaacaga ttttatttta catccggatg atccagcaga aatagatgca atcgtccaaa
     7261 aatatcaagg acagctaaga ctttaccaac aagcaatgtc agaagctttg aataagccag
     7321 tagaaaatgt tttcctaatt ttgcttcgag tgaaacaaat tataaacatt aacaaataat
     7381 tataattgag caaacttgac ttatgttttg taaggattat gctatactct gggaagaggt
     7441 gagtatgatg gagctaacac agacagaatt cgttttatgc cctaaatttg aaaaaacatt
     7501 ctctatttta ggaaaaaaat ggaacggatt gattatcgat gtactactcg aaaacggtcc
     7561 gcaacgcttt gttgagcttg cggcaaaaat cccgatggtc agtgatcgag tattagtcga
     7621 acgcttgaag gaattagaaa aagaaggaat agtcactcga aactttgcct gtagcgaaaa
     7681 gagccgttca gaatatctct tgactgaaaa aggagcagac ttgcaaaaag cgatgcgtca
     7741 aatacaagtt tggagcgaaa aatggatcac agaggaagaa tgcggttgat tttgtaaaaa
     7801 aaatcttgta aactatagtc catacgaata agtaaagaac gatgacgaaa aagagtagtt
     7861 tggctaaacc agttcaggga gatctgtcgc agactgaaag cagatcctgt gtttgaaaaa
     7921 tgaagttcac tttccgagtt cgtctgataa aatcagaccg gcaattgtcg ttaacgaagt
     7981 aagagtgcgg tgtaagtcat ttatccgcaa attaggatgg taacgcgaag acctcgttcc
     8041 ttaacaggaa tgaggtcttt ttgttttgta tttggaattt aaaggaggaa caagcacatg
     8101 tctctacaag cacaattaga gagcttgaaa gaagcaacgc tcgtcaaaat cacagcggca
     8161 caagatttaa atgaattaaa tcaaatacgt gttgaaacgt taggaaaaaa aggaccgatc
     8221 acagaagtat tacgtgggat gaaagatcta agtccagaag agcgaccaaa agtcggaagt
     8281 ttcgcaaatg agatccgaga tcttttgaca gaagagatcg agcgaaaaaa acaagtatta
     8341 gaagaagaag cgttaaatgc agctttggca aaagaaacaa tagacgtgac actacctggt
     8401 agaaaagtaa aacatggtac acgtcatatc gtgacacaaa tcatggaaga aattgaagat
     8461 atttttgtgg gtatgggcta tcagatcgta gaaggatatg aagtagaatc tgatcattac
     8521 aatttcgaac ggatgaatct gccaaaagat catccagcaa gggacatgca agatactttc
     8581 tatatttcag acgagatatt gattcggaca catacttcac cagtccaagc acggacaatg
     8641 gaaaaacatg atttctctaa gggtgctcta cgaatgatct cgccagggaa agtcttccgc
     8701 agagatacag atgatgcgac ccacagtcat cagttccatc aaattgaagg gctagttgtt
     8761 gataaaaaca tcacaatggg cgaccttaaa ggaacattag aagtcgtaat gaaaaaaatg
     8821 tttggggaag atcgtgaaat tcgtttgcgt ccaagttatt tcccatttac ggaaccatca
     8881 gtagaagtag atgtcagctg tttcaaatgt ggtggtgccg gttgtaacgt atgtaaatac
     8941 actggatgga tcgaaatttt aggagctggc atggtacacc cgaatgtgtt gaagatgtca
     9001 ggaatcgatc cagaagaata ttcaggcttt gctttcggcc ttggaccaga ccgcgtagct
     9061 atgttgcggt atggagtgaa tgatatccgt aatttctatc aaaatgactt acgtttctta
     9121 agtcagttca agggggagta agaaaatgtt agtttcatat aaatggctca atcgttatgt
     9181 agatttatcg aatgtaacac caaaagaatt agcggataag atgtctgtga caggaatcga
     9241 agtagaggga gtaacggttc cagaagaagg cttgaaaaaa atcgttgtcg gtgaagtcaa
     9301 ggaatgcgta ccacatccag attctgatca cctttctatt tgtcaagtcg atattggcga
     9361 agacgaactg tctcaaatcg tttgtggagc gcctaatgtg aaggttggaa tcaaagtgat
     9421 tgttgcactt cctaactcac gtatcgctgg taatgtaaaa atcaaaaaag gaaaaatgcg
     9481 tggccaagta tcaaatggca tgatttgctc attacaagaa attggctatt cagatagtgt
     9541 tataccgaaa gaatacgctg aaggtatcta ttatatgcca caagaagctg taaatggcga
     9601 tccagttttt ccttatttgg atatggatga ttcgattatt gaattatcta tcacaccgaa
     9661 tcgtgcggat gcattgtcaa tgcgtggcgt agcttatgaa gttggggcaa tttatcgtca
     9721 gactcctaca ttcgatgacg aaaagctggt agaatcctca cgtcctgcta gcgataaaat
     9781 tcaagtggaa gttgaagata aagaagcagt tcctgcttat caaatcagaa tcattgaagg
     9841 agtaagaatc cagccaagtc ctcaatggct gcaaaatctt ttgatgaacg aaggcattcg
     9901 tccaatcaac aatgtagtag atgtgacgaa ctatattttg cttttattcg gacaaccact
     9961 acatgcattt gactacgaca aattgaaatc aaaagagatc tttgtccgcc gtgcaaaaga
    10021 aaatgaaaca ttagtaacac tagatggaga atctcgggaa ttgacaactg acaatctcgt
    10081 cattactaac ggcaaagaac cagttgcttt agctggtgtc atgggagggt tagactcaga
    10141 gatcacgaat gaaacaacaa cagttgcact agaatctgct ttatttgatc caatttcgat
    10201 tcgtcgtaca gcaaaacaat ttaatttgcg tagtgaatcc tctgcacgtt ttgaaaaagg
    10261 aatcaaccat gcgactgttg gagaagccag tgaggttgct gcagcaatga tcgtttctct
    10321 tgcgggtggg gaagttctac aaggcgcggt caaaggctca gagatcaaag cagaagacgt
    10381 atcagtgaag atttcacttg aacgcatcaa tcaatattta ggaacatctc ttgtcgttaa
    10441 agaagtaaat gagatttttg aagcacttgg atttagctat gaagaaaagg agtatgttta
    10501 ttccgtagta gtaccaccaa gacgctggga tatccaaatc gaagcagata ttatcgaaga
    10561 agttgctcgt atttatggat acgaccagtt gccatctaca cttccaagtg gagaaacggt
    10621 tgctggaagc ttgtcaaaag aacaagaaac tactcgtaaa attcgtacaa tctttgaagg
    10681 aagcggattg agcgaagcaa tcagttatgc attgacaaca gaagaaaaat caagacagtt
    10741 tgttataaat gaaagtaata ttacacgttt ggactggcca atgagcgaag aacgttctgt
    10801 attacgaatg aatttgatca gcggactatt agatgatatc agttataaca ctgcccgaaa
    10861 aaatacagag attgctttat atgaaattgg tcgggtgttc ttccaagaaa atgatccacg
    10921 tacacatctg ccgcaagaag taaaacatgc agcaatcgct atttctggta tttgggaaga
    10981 aaaagattgg cagacgaaag ctcaaaatgt agactttttc accatcaagg gtcttttaga
    11041 aaacttattt gaacaactag gcatcactga agatgtccat tatcaaacag tgacagaaat
    11101 gaaggaaatg catcctggaa gaactgctgc gatttacctt ggcgaaaaat ttattggttt
    11161 tgtcggtcaa gtgcatccaa cgattacaaa aagttatgat attccagaaa catatgttgc
    11221 tgagtttgat ttatctgcta tagtggaagc tgctcaaaaa gggatcgttt tcgaagcagt
    11281 gacaaaattc ccagcagtca gccgggacat cgctttgctt gtaaaagaaa ctgtgacaaa
    11341 tcaggaatta acagaagtta tccgttctgc tgccggaaga tttttaacag atatccaatt
    11401 atttgatgtc tatcaaggag aaaacattga aaaaggacat aaatccatgg cgtatagcct
    11461 gacattcgtc aacccagaag caacattgac agatgaagaa atcaataaag caatggaaaa
    11521 agtcgaaaaa gcgttggtag aaaacctaga ggcaagtatt cgctaagaag gaaaatatag
    11581 taagtaacga tcaagtagct tttgaagacc atacatttga aaaaaagatg tgacacaact
    11641 tcgtcacatc tctttttttg attttaaaaa aggaatagca cagagacgag ataaaaaaac
    11701 aggcctttac agcctgcttt tttagagtaa atgaattaat ggaagtaatt gattcccatt
    11761 gctgatttta cttcagaaag tgtttgtgca gctactttag cagcttcttc actaccttta
    11821 cgtaagattt ccataacagc tgaaggatct ttttccaatt cttctcggcg cgcacggatc
    11881 ggtgcaaatt cagcttccaa tacatcaatg aggtatcgtt tgatctttac atcaccaagt
    11941 ccgccgcggc ggtaatgagc tttcatttct tcaattgctt ctttatctgt tccgaataca
    12001 tccaagtagg tgaagaccat attcccttct acttgtccag gatcttgtac atggatgtgg
    12061 tttggatcag tatacatact catgactttt ttctgtaaga catcggcagg atcggagata
    12121 taaataccgt ttcctaacga tttactcatt ttgccatttc catcgattcc aggtaatcta
    12181 cccattcctt ttggtgggaa tacgcctttt ggttccacca agacttcacc atatgtatga
    12241 ttgaagcttt gtacgatttc ctgtgtctgc tctagcattg gtttttgatc ctcaccaaca
    12301 ggtacaagat tcgctttgaa tgcagtgata tctgctgctt gagaaacagg atagataaag
    12361 aatcctgtag gcacgctttc accgaatttt ttttgttcga tttctgtttt gacagtcgga
    12421 ttgcgccgaa cgcgtcctac gctgactaag ttcaaataat acatggttaa ttcagctaat
    12481 tccggaattt gtgattggat aaataaagta gattttgcag gatctaatcc cacggctaaa
    12541 tagtctaaag caacttgcaa gacatttgat gaaaccttct caggattttt agcattatct
    12601 gtcagtgctt gcatgtcagc aatcatgaca aacagttggt tgtcttcatc tgcctgcata
    12661 gccactcggg tttttagaga accgacgtaa tgccctaaat gcagcttccc agttggtcga
    12721 tctcccgtta aaataatatt tttcaaaaaa gacacctctt tacgatttgt aagtctgagc
    12781 acttaatatc ttaccgtttt tcctgaaaag aaacaacagt tcctaaggaa aaaacaagga
    12841 aagaggataa aataactaat agattagaaa tggaagaaat atttatggga gaataaacgt
    12901 tctaagaaac tttacaaatt actaaactat cagatataat gtattttatt atacatcttt
    12961 aaaagaaatc ttactaaata ataatgggag tgtaattcta tggcaatgat taaatttgat
    13021 cacgtggata aatattatgg caagttccac gcattaaaaa atatcaatct cgaattcgag
    13081 aaaggagaag ttgtcgttgt cattggtcct tctggttcag gaaagagcac gatgcttcgc
    13141 tgtatcaatg gattagagac gatctcttca ggtaagctgc tgattaatga tactgacttg
    13201 catgataaaa agacaaaact gacagaagtc cgaaaaaaca tcggcatggt tttccaacat
    13261 ttcaatttgt acccaaataa aactgttctg gaaaacatca aattagcacc aatcaaagtg
    13321 ttgaagcaag accaggcaac tgcagtcaaa aacgcggaga aatttttgaa aactgtcaat
    13381 atgttagata agaaagattc ttatccttcc atgttatccg ggggacaaca gcaacgggtg
    13441 gcaatcgcca gaggtttagc gatgaatcct gagatgctgc tgtttgatga acctacttct
    13501 gctttggatc cggaaatgat tggtgatgta ttagacgtta tgaaaaaatt ggctcatgac
    13561 ggtatgtcaa tgattgtcgt gacacatgaa atgggctttg ccaaagaagt ggcggatcgt
    13621 gttatcttca tggctgatgg agaagtattg gaagacagca gaaatgtgag agactttttc
    13681 gaaaatccaa atgaagtccg agcacagcaa tttatcagta aagttatcaa tcattgatag
    13741 gaggaaaaag atgcgcaggt taaaaaaaat acgcctgatt ttccttttag gcattgcttt
    13801 tcttgcacta gccggatgta aaggaaatgg gttggcaaat gaagatattt tagagcgaag
    13861 caaatctacc aatgaaatca tctggggtgt caagtatgat actcgattat ttggaatgat
    13921 ggatattgag agcagaactg tacaaggatt tgatgtagat attgcaaaag caatcactaa
    13981 aaagatcctt ggtgacaatg gaaaaacaga atttgttgaa gtcacttcta aaactcggat
    14041 tccattattg aaaaacggaa atatcgatgc aattattgca acgatgacga tcacagatga
    14101 acgaaaaaaa caggttgact tttcagatgt ctatttcgat gctggccaag ctttgttggt
    14161 aaagaaagga agccaaatca aaagtgtaga tgatttgaat gcttctacaa cggttctagc
    14221 agtcaaaggt tctacatcgg ctgcgaatat ccgacagcat gcaccagatg caaaaatttt
    14281 agaattagaa aactatgcag aagcatttac tgctttacaa tctggtcagg gagacgcgat
    14341 gactacggat aatgcgattt tgttagggat tgcagatgaa aatccagagt atgagttagt
    14401 tggtggaacg tttactaatg agccttacgg gattgcgatc aacaaagggc aagaaaactt
    14461 cttgaaagct gttaaccaag cgctagagga aatgcatgca gatggcacat acgataaaat
    14521 ctatcaaaaa tggtttccaa atgaaactga aggaaaagta gagtaagggg gagaggcaat
    14581 gagcgaattg atacaaactt atggaccaat ctttgtcgac gggttcaaga tcacattgtt
    14641 ttcgagtgtc ttagcacttg tgtttagttt gattatcgga acgttaatgg caatcttcca
    14701 attatcaaag aataaagtag tgagcgcctt agcaaaggca tatgtagaat tttttagaaa
    14761 cattccatta ttaatcatcg tgatgttttt ttatgttgtc gtaccgattt attggtttaa
    14821 tctagatgga ttccaagcag gtacgattgg attaacgatc tatacatctg cttttattgc
    14881 tgaaacagta cgttcaggga ttcaaaccgt accaaaaggt caaacagaag caggactatc
    14941 ttctggtttt acctattcag aaatcatgcg ttatattgtt ttaccgcaag cattcaaaat
    15001 cgttatcccg ccattaggca accaatttat caacctcgta aaaaactcat ctattttagc
    15061 aatcgtagct ggtttagatt tgatgtatca aggagacttg atcgctagtg aaacattcaa
    15121 taccttcgat acgtatatca tcgttgggtt attttactta gttatcacgc taccgttatc
    15181 ttacttaatg gtttatcttg agaaaaaatg ggcggttcgt tcatagaagg aggggaaata
    15241 catggatttt agcggcgctt tttcatggat gaatatccgt tttttattgg aaggattaaa
    15301 ggttactgtt gaagtttcat tgttttcgat catttttagt ttcttgatcg gtggtctgac
    15361 aggtgtgatt cgttttgcga atatcccttt cttttccaaa atccttggtg tagtgattga
    15421 tattatacgg aatcttccat tattattgat cattttcttc acttactttg ccttacctca
    15481 aatcggcatt cagatgaaca tcttctggtc cgctgtcgca gcattaacga tttttgagtc
    15541 agctatgttg tcggaaattt tccgagctgg actcaatgct gttccaaaag gacagatgga
    15601 agcaggatta tcaacgggac tgacttatgt agaatcgatg cgaacgatcg tgttaccgca
    15661 agcatttaaa tcaatgatcc cggcaattgt gagccaattg atttcactga tcaaagatac
    15721 ttcattagca gtaatcattt ctttacctga actttctcat caggcacgta ttatttatgg
    15781 acagaacacc aattatgtgc ttccgatgtt catcatcatg acacttatgt acttcgtggt
    15841 atgttacgcg ctttctctgc tttccggtta cttggaaaag agacggtaca gttattaata
    15901 gatgattctt ttagcggttg tggcaaacta aagtcacaac cgcttttcct ctttccattc
    15961 gaaaaaaaga gatttcgatt gttttaagca agtaagagca cttgaaaatt cacgctaaaa
    16021 agtacacaat agggaggata gaatttattg aaaaaaagga tgatacgatt gacagataat
    16081 cgccctatcg gatttattga ttcaggtgtc ggcggcttga ctgtagtaaa agaagccctg
    16141 aaacaattac cgaatgaaaa tattttattt gtaggagaca cagcacgctg cccatatggc
    16201 cctagacccg cggaacaggt aatccagtat acttgggaaa tgacggatta tctggtggag
    16261 caaggaatca agatgctggt gatcgcctgc aataccgcaa ctgcggtggc tttagaagaa
    16321 atcaaagctg ctctttctat tccagtcatc ggtgtgatcc ttcccggtac tagagcggca
    16381 gtaaaaaaaa cacaaaataa acaagttggc attatcggta cgattggtac ggtaaaaagt
    16441 caagcttatg aaaaagcact gaaagagaaa gtaccagaat tgactgtgac aagtcttgct
    16501 tgtcccaaat ttgtttcagt tgtcgaaagt aatgaatacc attcatcggt ggcgaaaaaa
    16561 attgtggcag aaacattagc tcctttaacc actaaaaaaa tcgatacatt gattttggga
    16621 tgcacccatt atccattatt acgccccatc attcaaaatg taatgggaga aaatgttcaa
    16681 ctgatcgatt ctggagcaga aacagtaggt gaagtatcta tgctgttaga ttatttcaat
    16741 ttgagcaatt caccgcaaaa tggtcggaca ttatgccagt tttatacaac tggctctgcc
    16801 aaacttttcg aggaaatagc tgaagactgg cttggaatcg gacacttaaa tgtagaacat
    16861 atcgaattgg gaggaaaata aggatgcgtc atgacgggag aaaagcccaa gagcttcgca
    16921 aactggagct tcaaacaaat gttttcaaat atccagaagg atcggtagtg atccgttttg
    16981 gtgatacaac agttatttgt tctgcaacaa tagaaaattc tgttccccct tttttgagag
    17041 aaacaggaac aggatgggtg acagcagaat atagtatgct gcctcgagca acgagtacga
    17101 gaaatcgtag agaaagcagc aaagggaaac tttctggtag aacaatggaa atccagcgtc
    17161 ttattggtcg ttccttgcgt gcagtagtag acttggaaaa attaggagaa cgtagtatca
    17221 tcgtagactg cgatgtgatc caagcggatg gcggtactag aacagcaagt atcactgggg
    17281 catttgtggc attgaaatta gccattgaaa aacttttaag agaaaaagaa ttgtcagaag
    17341 atccaataaa agaacatctt gctgcagtta gcgtaggaat cttaccagat gggacttgcg
    17401 tcacagattt ggattaccaa gaagattccg cagcacttgt agacatgaat cttgtaatga
    17461 cagaatctgg aaaattcgtg gagattcaag gaactggaga agaagcaacg tttgatggtg
    17521 aacagttgaa tgaaatgctc ttttttggaa aaaacgcgat tgaagatttg atcaaagagc
    17581 aaaaacatgc tttgctaaca gagtttgctc aaaatgatga gcggatagaa gaaacaaaaa
    17641 caatcatcat tgcgacaaga aatccaggaa aagcagaaga gtttcgaaat atgttcaaag
    17701 aagcgggata tcatgtaaaa acattacttg actatcctga attgccagat gtggaagaaa
    17761 ctggcagcac atttgaagaa aatgcccgat taaaagcaga aacgattgca caattgcttg
    17821 atcagccagt cttagcagat gattcaggtt tgaaagtgga tgctttaggc ggtatgccag
    17881 gaatctattc tgcacgtttt gcaggggaac aaaaaagtga tgcggggaat aacgccaaat
    17941 tattatatga attgacagat gtgccggatg aaaaaagaac agcacaattc cattgtacgt
    18001 tagtattcgc ggcacccaaa aaagacagcc ttgtagtaga agcggaatgg cccggtcgag
    18061 tagcccgtat accatctggc gaaaatggct ttgggtatga tcctttattt atcccagaag
    18121 gtaagaaaca aacagcagca gaattatcga gcgaagaaaa aaacaagatc agtcatcggg
    18181 ctcaagcaat gaaaaaattg agtgccgagt ggaaacaatg gctggaagga gagagataga
    18241 tgcggtattt agtagtaagt gataatcatg gagacagaga tatcctcgtt gacttagtag
    18301 atcgttatcg ttctgaagtg gatatctttt tccattgtgg tgattcggaa ttagaagctt
    18361 ctgacccatt atgggataca ttccaagtgg ttcaaggtaa ttgtgattat ggaccaggat
    18421 tcgaacagaa aaaagtgatc caaactggtc aggatacagt attcatgact cacggtcatt
    18481 tatcgaatgt ccgatttggt ttgacacagt tagcaatcga agctcgagct gcaactgccg
    18541 atatggtgtt ttttggtcat acccatcaaa taggctgtga gatggatcag aaaatcctgt
    18601 ttttgaatcc tggtagcatt tctcagccta gaggcccgat ccaaatccca tcatatgctc
    18661 ttatcgaaag tttagatgac caaatttctg ttcaatacta taaccgtgcc catcaggcag
    18721 ttgaagagtt ggccttttct ttttcgagag agcgctgaat aaaaaaactc taatttttaa
    18781 taaaaataag ctttatttta tgaattttgc atccggtttc ggtaaaatag tagtgttgaa
    18841 tgaaatagaa atggaggcca tcagatgatt ggacctatag taagagaaat gctgctggaa
    18901 aatcaggaaa cgtttttgat tcctgcagaa aatgtggcaa atgttatgta tcatcatcca
    18961 ttgtcccatg gtttacttgt gttatcgaag gtcggttatt ccaaaattcc tgtattagga
    19021 aaagatgatc actttgtagg actagtaagt ttatcagatg tggtcaataa aatgattgac
    19081 ttgcaaacaa tcagcatgga cccgttagaa ggactaacgg ttgctgatgt gacagaaaca
    19141 gatgttccga caattgatga aaattgggaa ctagaagaag tactccattt gttagtcgat
    19201 tcgccttttt taccggttgt cacagaagat cggattttca aaggaatcat tacaagaaaa
    19261 gagctgctta aagccgtaaa cttcatggtg catgaattag aacgtcgtaa tatattgtct
    19321 ccgaaaatcg aagaattaaa agaacaaatc gatatcgtga gctaaagaac gagaaactaa
    19381 aagataatca aaaaataagt agaaaaaaca gcgtaccagt tttgacttta tcttgaaaaa
    19441 gaggctggga caaaagcgtt taactccaag aaataagaag gaattcacga aaattgcttt
    19501 tcaaattttt gtgaatttca gcttatttcc gaaggagttg cttctgcttc cgccgtttat
    19561 acgtgtttag agcgtgggac aaaagtgttc tttacttttg tcccacgctc tttttagtga
    19621 gattcgttgc atcgtttgtc caaattattt tccgacagca attggactgt tcggaatcgt
    19681 gaaaccagaa gctgtcaatt ctttgacata ggagctcaag aactcttctt ttaaggcata
    19741 ttgttttccg ttcaatgcgt agcagaccgt acgaatcgca aaattgctgt ttccaagatc
    19801 cactaatcca aagatagaag gttcagtttg gagctcttct tggtattttt cagctaatgt
    19861 acgatttact cgatcaatga tctcatagat tttgtcatat ccttcttcag gaacaatccg
    19921 aatatctaaa agtacttgca tatttgcacg agagagattg ctgatcgtgg tgatgtttcg
    19981 attcgggatg aaatggacag taccatccgt tgctttcagc tgaagggtac ggataccaac
    20041 agatacgacg gttccttcaa tcgttaagtt tgctagcttg atgtagtccc caacatccat
    20101 ctgttgttcc atgataataa aaaatccggt aataacatca ctcatgaacc cttgggcacc
    20161 aagaccgatt gccacacctg caattccggc accagccaaa agtgatccga tcggcacacc
    20221 gatagcagat aaaattgcat agataaaaaa gaaaccaagg gtataatgaa aaatagtatt
    20281 taaaagacta tgtaaagtct ttaagcggct ctcgtttaga atggcttttt tagaatactg
    20341 attaaaagac ttatcaatca gatattttcc cacacggtga agaatccaaa aaaggacgat
    20401 caaaaaaatc agatataaac ttttttggat aaaggcagcc aaaatggcgt cccaatcaat
    20461 cccgtcccaa aaccgctgaa acgcatttac tttgctgaca gcttgatctg tcaatgtctc
    20521 gccagaagag tctgtcgtac cggcagttgc taataatgaa aacataaaat cctcttttct
    20581 aaaatttgat tctttctgta ttgtaccaag aagttttcaa aaaaggaaat agcaaaaata
    20641 actataatga aggaaaaaag ctgaaaataa aatgacaaat aatatggata atgataaaat
    20701 gtggagaaaa taacgaaggt ggtaagtata tgcaaatcga ttggaacaat ctaggttttt
    20761 cttatatcaa aactccttgg cgttttattg ccaaatggaa agacggacaa tgggaagaag
    20821 gagaattgac agaagataat tacctgacga ttcatgaagg ctctccagcg cttcattatg
    20881 gtcagcaatg ttttgaaggt ctaaaagctt atcgtcgaaa agacggcaag gtcaatctct
    20941 ttcgacctga acaaaatagt cgacggatga atcaaagtgc tagacgattg ctgatgccaa
    21001 tggtccctga aaagatgttc gttgatgctg tcaagcaagt ggtaaaagca aatgaagatt
    21061 ttgtgccgcc ctatggaaca ggcgctacat tatacttgcg ccctttactg attggggtcg
    21121 gtgaaaacat tggtgtccat ccagctccag aatatttatt tgtcgtcttc tgtacgccag
    21181 taggagcgta tttcaaaggt ggactaaaac caacgaattt tattgtatct gattacgatc
    21241 gtgctgctcc taaggggact ggtgcagcaa aagtcggcgg aaattacgca gcgagtttac
    21301 ttccaggaac agaagcaaaa gaactggatt actcagattg tgtttatctt gatccagcca
    21361 cacatacgaa aatcgaagaa gtaggtgcag caaatttctt tggaattaca aaagacggaa
    21421 aattcatcac tccgcaatcg ccctctatct taccaagtat cacgaaatat tccttgttgc
    21481 agttagctga agaacgacta ggactgcaag caattgaagg agatattttc attgatgatc
    21541 tagatcaatt tgcagaagct ggtgcatgtg gtacagcggc tgttatttct ccaatcggtg
    21601 ggatttatca caataacaat cttcatgtat tttatagtga aacggaagta ggaccagtaa
    21661 caaaacggtt gtatgatgaa ttgacaggta ttcaatttgg agacatagaa gcaccagaag
    21721 gctggattga agtcgtggaa taatttcaaa gtaatgagtt gaaaaaaacg taaaaagaaa
    21781 aaatcaggaa gagactttaa aaagtttttt cctgattttt atatgtttta ctttaaagaa
    21841 tgcaacaaaa gtttatgaaa gatggacacc tttgtcaacg tagataatat caccgatgat
    21901 tccgctggaa agtgggctga ttaagaaagc acaagcatta ccgacttctt cgatcgtcac
    21961 accttgctga tcaggcgtac gagattcaga taactggatc agtttttgat agtctttaac
    22021 acctgtgaca gctaatgttt tgattgcccc agctgagatt ccattgaccc gaattccttt
    22081 aggcgagaac tcagctgcca aataacggat agcagcttca agagaagctt tcgcaatacc
    22141 catcatgtta taattcggga ttgcgcgttc tgaacctaga taagtcatgc taacgatccc
    22201 gctgtttggt gcaagaaacg gttgtgcgta ttttgataca gcaattaaag agtagctgct
    22261 gatatcttga gctaattgat agccgtctct ggaaatatca gacacatttc cggacaattc
    22321 ttctttgttg gcgtaagcaa ctgcatggac aaggccgtgg atctccccag catattcacc
    22381 gatggcattc attgcttttt cgatgctttc gtcgctagaa acatcacatt ctacagtgaa
    22441 tgcttcttcg ccgaccaatt tgactaatga ttttttcatt cgttcatttt ggtaagtata
    22501 gataatttct gccccttgtt tcaacattgc ttcagcacaa ccccaagcaa tgcttttttt
    22561 gttagcgacg cccataatga cgattttttt tccttctaga aatgacataa tgactctcct
    22621 ttgtttctga taaaaatttg atagtcgtaa aaaaatagag aataatagct aaaataactt
    22681 cttgatttct taattattag tcaaaatgtt ttgattgtca aactattttt tcttaaatta
    22741 aaaaaagatt gctaaatatt taggaatatg atactttgat agtcaaagta atgaaaatgg
    22801 caattgcctg aaagaaggag tttaattatg tcttacgtat tatctgctac tgaagtaatg
    22861 gaattgattc ctaatcgtta tcctatttgt tatattgatt atgtagatac tttagaacca
    22921 ggaaaaaaga tcgttgcgac aaaaaatgta acaatcaatg aagatttttt ccaaggtcac
    22981 tttcctgaca atccagtgat gccgggtgcg ttgattctag aaacactggc acaagcaggt
    23041 tcgatactta tcctaaaatc agaagagttt ctaggaaaaa gagcttatat cggtgggatc
    23101 aataaggcca aattccgtca aaaagttgtt ccaggagatg tgctgaaatg tcattttgaa
    23161 atcaccaaaa tgaagggatc agtcggtaca gcgttatctg cagcatatgt agatgataaa
    23221 aaagtctgtg agtgcgagtt cacgttcatc gtcaaccaaa atgcttgact ataaaacctg
    23281 atatagcaaa acaaaaggaa gcttacttgc tttttatgta aaaagaagac gcctgaatcc
    23341 tagcatacgt tgtcatctat gcaggattca gacgttattt ttttattttt gttttgcttc
    23401 ttgcagtata ttgattttat cgatttttac ttcttctttt ggtttgtctg aactatctgt
    23461 ttcgatattt gcaatagcat ccacgacatc cattccttca atcacttggc cgaagacagt
    23521 ataatggcca tctagtgaag gataaccgcc gtttttataa gcgtcgatga ttgcttgagg
    23581 atagtcgtct tttaaaagac catcatgcat atcatctgta ttttgaacga taaagaactg
    23641 gctgccgtta ctgttaggat cttgtgagcg agccatagat aacgcacctc ttatattata
    23701 aaggtggttg ttgatctctg tttcaaatcc ttctccccaa atgctttccc ctccagtgcc
    23761 atcgccttta ggatcgccac cttggatcat gaaatctttg atgacgcgat ggaaagtcag
    23821 tccgttgtag taaccgtctt ttgcatgtgt cataaagttt tcaaccgctt ttggtgcgtg
    23881 ttcgggaaat aatttgattt tgattgtgcc tttattcgtc accatttcga ccagatcttc
    23941 attttcggcg acatctttcg tcaattgtgg taattccaat gcgttcaaat cgacgctact
    24001 ggcagtcgtg ctttccgcag tagatgtact ttttgaactt tctgtagacg aactagaaga
    24061 agtcgtgttg tttgcagaac aaccagcagc aaacagcaat gctgctgcta aactaatact
    24121 taaaataact ttatttttct tcataactgt tcctttccaa tttactattt tgttatcttt
    24181 aatgataaca aaatagaggg aaaataccta cttaaattat tggaatttat catttatttt
    24241 aaaagaaaaa gaaaaggtgt agaccattta ctttgtgttt gctataatgt aagtggatac
    24301 aaaaacgaaa agatgcagga ggaaatggta atatgggaaa attaggaatc tcgatttatc
    24361 cagaacggtc aacatttgaa aaggacaaag cgtacttgga tttagctcat aagtatgggt
    24421 ataaacgggt ttttaccagt ttgctgcaga tcaatgatga taaagaaaaa gttctttcag
    24481 aatttaaaga agtagttgat tacgcgaata gcctaggcat ggaagtaatg gttgatatca
    24541 atccagcgtt atttgaacag ttggagattt cttacgatga cttatctttt ttccataaga
    24601 tgggtgccta cggggtccgc ttagatatcg gattcacagg agcagaagaa gcaaaaatga
    24661 cgcgcaatcc ttttggtatc aaaatcgaga tcaatatgag ttcgggcacg aattacgttg
    24721 acaacatcat gagttattcg ccaaacacgg ataatttatt aggttctcat aatttttatc
    24781 ctcatcgata ctctggttta ggatacgaac actttgtttt ttgttcagaa aaattcagaa
    24841 aatataattt gaatacgatg gcattcgtca attcgcaatc tgctgagttt ggcccttggc
    24901 ctacgcaaga tggcttgtgc acgctggaag accaccgaga tcttgaaatt gcgacacaag
    24961 tgaagcattt aatccttaca ggattgattg atgatatttc tattggaaat gcgtacgcaa
    25021 gtgaagaaga attgaaagaa atggcagaag catttaacgc ggattatcca acattgaaag
    25081 tagatacgga agaaggtatt acagaaaacg aacgaatctg cttgtttgat aaccttcata
    25141 gttatcgagg ggatcgctca gaatatatcc ttcgttcaac gatgactcgt gtatactaca
    25201 aagataaaga tttcccgcct cataatacgc gagatatgca tcatggggat gtactgattg
    25261 acaatgaagg gtacggtcaa tacaaaggag aaacacaaat cgccttgaag gatatgaaaa
    25321 acgacggacg tgtcaatgtt gtaggaagaa tctccgatga cgaattattc ctccttgact
    25381 ttttgaaacc atggtcaagc tttaaattaa ttgaaaataa aaaatagata attagttatt
    25441 gcagaaataa tccgctagaa atagccagtt atttctgctt ttttattttg tttttttata
    25501 gg
//
LOCUS       DACDXO010000035        25401 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E23605_2017
            SAMN12639989-rid9881463.denovo.035, whole genome shotgun sequence.
ACCESSION   DACDXO010000035 DACDXO010000000
VERSION     DACDXO010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639989
            Sequence Read Archive: SRR10023389
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741914.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:42:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,972
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 33 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 17 of 73
            Pseudo Genes (multiple problems)  :: 10 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25401
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639989-rid9881463.denovo.035"
                     /isolate="E23605_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(205..1104)
                     /locus_tag="IWM35_001640"
     CDS             complement(205..1104)
                     /locus_tag="IWM35_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HAP9919496.1"
                     /translation="MGKLAIISDLHVDINKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
     gene            complement(1114..1470)
                     /locus_tag="IWM35_001641"
     CDS             complement(1114..1470)
                     /locus_tag="IWM35_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAP9919497.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            1825..2121
                     /locus_tag="IWM35_001642"
     CDS             1825..2121
                     /locus_tag="IWM35_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit alpha"
                     /protein_id="HAP9919498.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLA
                     SKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
     gene            complement(2765..3718)
                     /locus_tag="IWM35_001643"
     CDS             complement(2765..3718)
                     /locus_tag="IWM35_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP9919499.1"
                     /translation="MKKLVVGILILASFGLAACGNSNNTSQADTKKSSTQTETTLTIT
                     DSNGDQIEVPNNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(3741..4496)
                     /locus_tag="IWM35_001644"
     CDS             complement(3741..4496)
                     /locus_tag="IWM35_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP9919500.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHLGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKNLVQEIGKTIVIVLHDINFAASYADEIVAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            complement(4493..5452)
                     /locus_tag="IWM35_001645"
     CDS             complement(4493..5452)
                     /locus_tag="IWM35_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HAP9919501.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFACALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            complement(5445..6395)
                     /locus_tag="IWM35_001646"
     CDS             complement(5445..6395)
                     /locus_tag="IWM35_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HAP9919502.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFLQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            6545..6997
                     /locus_tag="IWM35_001647"
     CDS             6545..6997
                     /locus_tag="IWM35_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HAP9919503.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPAEKEKPKKKKSFWDRLF"
     gene            complement(7086..9353)
                     /gene="gshAB"
                     /locus_tag="IWM35_001648"
     CDS             complement(7086..9353)
                     /gene="gshAB"
                     /locus_tag="IWM35_001648"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HAP9919504.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEEIIIAKLKNFEDVLYRRYLAKEYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDSIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(9540..10538)
                     /gene="ftsY"
                     /locus_tag="IWM35_001649"
     CDS             complement(9540..10538)
                     /gene="ftsY"
                     /locus_tag="IWM35_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HAP9919505.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKTEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            complement(10549..11358)
                     /locus_tag="IWM35_001650"
     CDS             complement(10549..11358)
                     /locus_tag="IWM35_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP9919506.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            complement(11367..14948)
                     /gene="smc"
                     /locus_tag="IWM35_001651"
     CDS             complement(11367..14948)
                     /gene="smc"
                     /locus_tag="IWM35_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HAP9919507.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLMKAAAEKETEIQKAEANLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYIQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIETALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            complement(14968..15654)
                     /locus_tag="IWM35_001652"
     CDS             complement(14968..15654)
                     /locus_tag="IWM35_001652"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HAP9919508.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            complement(16002..17783)
                     /locus_tag="IWM35_001653"
     CDS             complement(16002..17783)
                     /locus_tag="IWM35_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP9919509.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            complement(17809..18723)
                     /locus_tag="IWM35_001654"
     CDS             complement(17809..18723)
                     /locus_tag="IWM35_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9919510.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            complement(18750..19712)
                     /locus_tag="IWM35_001655"
     CDS             complement(18750..19712)
                     /locus_tag="IWM35_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9919511.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            complement(19712..20656)
                     /locus_tag="IWM35_001656"
     CDS             complement(19712..20656)
                     /locus_tag="IWM35_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9919512.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            complement(20657..21661)
                     /locus_tag="IWM35_001657"
     CDS             complement(20657..21661)
                     /locus_tag="IWM35_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9919513.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
     gene            complement(21929..22171)
                     /gene="acpP"
                     /locus_tag="IWM35_001658"
     CDS             complement(21929..22171)
                     /gene="acpP"
                     /locus_tag="IWM35_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HAP9919514.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
     gene            complement(22293..23294)
                     /gene="plsX"
                     /locus_tag="IWM35_001659"
     CDS             complement(22293..23294)
                     /gene="plsX"
                     /locus_tag="IWM35_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HAP9919515.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            complement(23343..25379)
                     /gene="recG"
                     /locus_tag="IWM35_001660"
     CDS             complement(23343..25379)
                     /gene="recG"
                     /locus_tag="IWM35_001660"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HAP9919516.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKQFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LITGSTKAKERRELLEKLEQGEINVVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICLLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
BASE COUNT         7031 a         5474 c         4480 g         8416 t
ORIGIN      
        1 tatatttttt tttgtgatta tcgttcaaaa aaagaataca cgcttgcatc tagtactctg
       61 cgagttacaa atcatccgat caaataaaca ttcgctgatt attttccgca aaaaaagcag
      121 catatccacg ataaatggat ctactgctct tacttatcct tatgttttac aaggtatttt
      181 cttttacaca ttactaatgc tgtctcaata cggaacgatt gtcatcgctt gttcaaattc
      241 ctcttgcaaa tgttgtgttt gataggcctg aaaagctggg tgatgggcac gcaagaggac
      301 acgtaatttc ataggatcat agctgtctag caactgttct ttcaagacaa aatctcgggt
      361 cagctgccat tcataataat aaccaaaagg tctgcagctg taaatcgtgt gaccgatgga
      421 tttatcttca aagcgacgat gcgtatggcc aaaaactact tgtttgatat ttgaataacg
      481 gtcaagtaat tctccgaatg attctgatcc taaaaatgca tttaatttgt tccaacgctc
      541 ataaggagca gattgataca tgataaatgc ccgtttagga acgaaatgag tggataaaat
      601 cacctcaagt ttttttgctt ccagctggtc tagcaaattt tttgcagctg caagcatttc
      661 tgcattgacc tcgagatctg ttttcccacg ctggatgaac cgatcatacc aatacaaacg
      721 cttcattcgt acgattgcct cgtaatcttt caattcggaa aactgatagt cgtaccagcc
      781 attcatccct aaaagaacgg tctgctcatt taaagcaata taccgttcat ttaaaaaatg
      841 tgggtctgga aaatggtcca tcatctgttc attgggcaca tctgccaact cgtgattgcc
      901 gaaatgaaat gtcgtagata atcctttatt gacgaaaaat tcgacaacag ctaggcaacg
      961 gtctacttta ttagccgtat ctccggctag atgaagccga gtgatcttgt gcttttgcaa
     1021 aacttgccaa agctgcatca gctctgattc gccgaattta ttgatgtcca catgtaggtc
     1081 gctgatgatc gccaattttc ccattttatc cctctagtct tttcgaacaa cttgcaagtt
     1141 gccttttctc aagccgcttg gtccttttaa ttgtaaacta ttataagttg gttctaaagc
     1201 aagtgtggat ggatcatcca tgtaaagtat tgcactgtct ttgattttga ttggtccaac
     1261 tggtgtatca gccactacag aataaagact tagatctgtt tgtgtatctt ttttgacaat
     1321 gatcaatcga aatgccgtat ccaattggca actgacttgg ctttctgcaa atggaccgtc
     1381 accgttttcg atatctaata aaagttgatc gtctgcttct aatttattct tcaatacttc
     1441 ttgtgcttct tttgtcactt ctaatttcat ctgtatcacc tcatgtcata gaataatcac
     1501 tttattcatc agatgcaact aatttgtctg gttctgactg ttctcaattt attggaaagt
     1561 aagtcattcg tttattatga tttctcttat cacaaaagag acaactttcc cttttcttcg
     1621 ttcagtttct tgaacggaaa aagacccaaa aagcaacctg ttatttgttt aaccttattc
     1681 ctttcttaaa taacaaaaaa tactataaaa aataaaaaac ctttaacaac tattggaaaa
     1741 agaattactt tttgttcacg gattattaac aactcttcgg tagagtaaac ttatcaaata
     1801 aaagacttct ttggaggaat cactatgaag gaatatataa aaaacatcta ttttatcgaa
     1861 gaaacacaaa atatcgaggg ttcttatatt gaagtaaaaa cactctttgt caatgaagat
     1921 aaaaccaaag cattggatat ttataaaaaa ttagcttcaa aaaaaacgaa tagttttgga
     1981 ttgatattaa gcgagtataa aatcaaagca gaagagagtt atttctatca attattaaaa
     2041 cggtggagta agcttcccgc cgatttttat cgaaaaatgc agatcatcaa ctatcaaccg
     2101 cttgctgaga cacatgccta aatagaggtt gtgaaatcag tgctttgagc taataccgaa
     2161 ggtcagctga ttgaacattg tttagaagag attgtgacaa ctgcgatttg ctttgagtaa
     2221 taagaacgct ggacagaaaa tagcttctca tattttctag tctagtgggc ttattgccga
     2281 gaagcagcag atggaacatc gtttagaaga gattgtgaca actgcgattt gctttgagta
     2341 ataagaacgc tggacagaaa atagcttctc atattttcta gtctagtgga cttattgccg
     2401 agaagcagca gatggaacat cgtttaaaga agagatcggt acaaaataaa aatatctaaa
     2461 tttaagtttt atctaagttt cgctttgtac ctttgattca aatcaaagga ggcgatcttg
     2521 atgctgaaaa aatggatcac actatttttt gccactcccc attctagaat caacccgtat
     2581 gatgtagata atgaattcac gttgaaaaaa gatttctcaa ataaataaaa accatccgta
     2641 aaatcatcaa tacgatttta cggatggttt ttgataactt ctttgaggat aggctcttgc
     2701 tatttaagaa tgtaagaaac attattgtta catagtatga gagtttaatc ctctgttatt
     2761 tatgctattt aaatgcttga ttcacatctt cgatcatcag tttcattgat tctaaaccgc
     2821 ccccgcttaa ataccagaca tctggttcaa gggagatgat ctgctgattt ttaccagcat
     2881 tcgtttgtgc taccagttca ttggcagaaa tatcatcttt gctgtcgtct ccaccaatag
     2941 cttttgttct gtctactaca aataagatat ccggattttt ttccagcaca tattcataag
     3001 aaacactttg accgtgagtg gatgcttcga tttgatcatc tgcttgttca aagccaaata
     3061 aatcatggat gaaaccgaat ctagaaccag atccgtaggc tgataattgc ccttcattca
     3121 caagtgtgac tagtgctttt ttgtcagtag cttctgcttt ctttttcgtc tgatcgattg
     3181 cactagataa ttcctctaat tgagttttag cttcttcttg cttaccaaag atctccccta
     3241 aagcagtgac attttgctgg aaagattccc atggattttt cgcatctaaa gaaaggaaaa
     3301 tggtcggagc aatttgactt agatcttttt gaaaatctct ttgtcggcca gaaatgatga
     3361 ttagatctgg ctgaagctga ttaatttttt ctaagtctgg ttcttttatt ccacctgctg
     3421 attctacttt tttataggat gaaagatact caggaaggct gcttgtagct gctccaacta
     3481 ccttatctcc aacaccaaga gcatccatcg tatctaaaga accattgtcg aatacgacga
     3541 ctttttctgg attatttggc acctcgattt ggtctccatt actatctgtt atcgtcaatg
     3601 tagtttcagt ttgtgtgctg cttttttttg tgtctgcctg actggtgttg ttgctatttc
     3661 cacatgctgc taaaccaaat gatgctagaa tcaagatacc tactactaat tttttcatct
     3721 cttctctcct aatgttagtt ttagaaataa agacagaatc tgcggccttc aatctcacaa
     3781 atccgtatgt ccatttcgta taattcatcc aatacatctt tttttatcat ttcttgggtc
     3841 gttccttgcg taaataactg tccatctttc attgcaacaa tttcgtctgc ataacttgca
     3901 gcaaagttga tatcatgaag aacaataaca attgttttcc cgatttcttg tactaaattt
     3961 tttaaaatct gcatgatttg aaccgcaaaa ttcatatcta gattgttcaa aggttcatcc
     4021 agtaaaatat aatccgtgtc ttgcgctagc accattgcaa tatacgcacg ttggagttga
     4081 ccgcctgaaa gagtgtcaat cgattgatcc gctatttctt ctaacccaag gtgttccagt
     4141 gattcctgaa cgatttcctc gtcttttgtc gtcaattttc ctttcgtata aggaaatcgt
     4201 ccaaattcta ctaattcttt cacggtgatt tggacattta tttcattcga ttgtcgtagg
     4261 atcgataatt ttttggctaa ttcattggat ttccaagcct ttacttcgtt atggtctaaa
     4321 tagatttcac ctgtatcttt tggtatcaaa cgactgatca tcgacaacac cgttgacttt
     4381 ccagcaccat ttggaccgat aaaagcagtc aatttctttt caggaatcgt taactgtaca
     4441 tctttaacca ctgattttcc atggtacagt ttattgacct tttgtaaatc aatcatagga
     4501 tactcttcct ttcgcgaatc aattttgtga caaaataaat tcctccgaca aattcaacga
     4561 cgacactcac tgttgtattc cagccgaaaa tctgctctac tgcgagctgt cctcctgtca
     4621 gaaacagcat agctaccaga aagcttccga taaatagctg accatgacga taagtagaaa
     4681 agacacgata actgatattt gcgacaataa accctaaaaa agtaactgga cccacaagag
     4741 ccgttgatgt ccctgtcaaa agactgacac aacataaaat caaaaattca aaacgatctg
     4801 tgtctatccc gaggtttact gcttgttcat ttccgagatg gaaaacgtct agcacatgtc
     4861 gatatcgaaa taacaggtac cagcagccga taatgatcaa agaagcaggc aaaagatact
     4921 gactctggat attgccaaag cttgcaaata atctgccttg aagcaaatca tattcattcg
     4981 gatccatgat gacttgtaaa aaggtactga cactaccaaa aagcgtacct aaaatcatcc
     5041 ctgacatcag taataaaaaa agattcgctc tcattttttt caaaagaaag aaaaataaca
     5101 atagactcag cccgaccatc agcccaacgt tcaataagaa aaaaactgta cctgtctgct
     5161 gaagcatcgt cactcctccg ataaagaaga aaaggaccgt ttggacaagg acatataacg
     5221 actcaagtcc caatactgaa ggagtcaaaa actggttatg cgtaattgtc tgaaagctga
     5281 ttgtggcact gctgacagct agcgagacaa ggataaatgc aaaaattttc ttactcctca
     5341 atgcacatgc aaaatgccag tttccatatg tatggatcgt caagtacatt cctgtaacaa
     5401 ataaaacaag aacaagcata ccaataaaaa tagatggctt ttttttaagc atatcgtcgt
     5461 ccccttatca gaagataaag gaagatagcc ccaccgatga ttccaacgat cacactgact
     5521 ggtatttcat aaggaaaaat caacacgcga gcgacaagat cgcaaaatac taagaacaaa
     5581 ctacctgcta aagcagttac gccaattgtt ttgtgcactt gatctccata tattctagag
     5641 acaagattcg gaatgatcac tccgataaaa ggaatgcttc ctaccatgat caacatgact
     5701 gcactagcca atgatactaa gccaatgcct aaaaactgca tcttctggta atctattccc
     5761 agatttactg caaacgattc gcccagacca atgatcgtaa atcggtaagc aaatagccaa
     5821 accaagagca acaatggaac aacagcatac aataactcat aatttcctcg tatcacagtc
     5881 gcaaaattcc cttgaagcca tgaagtcata ttttgtacca actggaattg ataagcaaaa
     5941 aaagtagcaa ctgaaccaat gatattccct aacatgactc caatcaacgg aagcatcaat
     6001 tgatttttct ggaggaacaa tcgcgtaagg gataaaaaaa gcaatgttcc tgcaaaagca
     6061 aagcaaaatg ctgtcaagga tcggacaatc aaggatgcac caggtaggaa aatcatagcc
     6121 actaaaatac ctaaacgcgc actatccatc gtgcccgcag tcgttggtga aacaaattta
     6181 ttttgggtca aatgttgcat gatcaaccca caaatactac tggtcgctcc cacaaggatc
     6241 aaacaaatcg ttcgtggcaa acgagtcact agcaagattt gtaagctttg cccatctaac
     6301 tggagcagtt ctgacaattg tacattcgat actccgatga acaatgaaat actacttaaa
     6361 ataactaata atgctactag tcgtccgtag cgcatccaat ccacctttct aattgagaat
     6421 gattttcatt cttattttaa gtgaatcgtt gaacaaaagc aaccgctttt tcatatgttt
     6481 taggtattat tgggaatatg ctataatcgt aagaaagaca aagagaagaa ggagggtatt
     6541 ttttatggca caagatgatg taaaaagcca tttggataag ggactttatg gtacaccttt
     6601 agtgaaccct gaagaacagc ataaatattt agggacattc agggaacgct gctatcttag
     6661 tatgactgtt gctgaaatga tccaaccaaa aaacaaagaa aactttttaa aagaactcaa
     6721 aaaacatcca gatgcttcca tactcttgaa tggggctcta cccctttctg tccaaaacca
     6781 atatatccag ctagcaacga aaaacaatag ccgtttcact gtagtcaacg attacgtcac
     6841 tgcaaatcct gaggaattcg gattgctctt aacagcaaaa gaagcagtaa atgaacctgt
     6901 gattgatatc gaaaaaaaat acccagaaga aaactcttcg ccagctgaaa aagaaaaacc
     6961 aaaaaagaaa aaaagcttct gggatcgttt attttaaaac gaaaaaacac ataaattttt
     7021 acatgcaaaa aaagcttttg atcagattga catcaaatag gacaatctgt caaaagcttt
     7081 ttcttttaaa atacttcagg atataacaat ttcaatacat tcatcgtcaa tcttctgcct
     7141 tttcctgcaa aaggataaac atgcatatgc atggctggat tgaaatttgc ttcgatgatc
     7201 ccatatgctt ttttatcagt tgtaggatcg atttctttat ctggtataat caaatcgata
     7261 ccactaatct tagctcctag tgcttctacc gcagaaacgg cgattttttt gtaagcttca
     7321 gaaaattcat cagtcatatc gatcgaatca ccacctgtac tgatatttga gttctccctt
     7381 agataaacaa tttgattggc ttggggaact gattcgatcg tcaacccttg ttctttcaac
     7441 atcaattgtt ctaattcacc taattggatc aattctaaag gtgcacgatg attcgttcca
     7501 cgcaaagggt cactattttt ttcttcaaca agttctttca ctgttcggat actgtcacca
     7561 ataacattag cagggactcg caacatgatg gcttggactt ctccgtcgat cacaaagaaa
     7621 cgatattccg ttcccggcat aaattcttct actaaaaccg aactgtcttc acgaaaagca
     7681 atggctaaac ctgcttgata atcttccaga ctagctcctt ccttgaaaat cgtgatacct
     7741 aacccgtaat tggttgattt aggtttgatc acaaatgcct gctcggcaaa tctcggataa
     7801 gcttttaccg cttcttccat ggatgaaaat tctgcgcctc ctggtacccg gaacccagct
     7861 tcttttaaga cttttttcgt tactgttttg ttttccatga taagtgggac gatatagctg
     7921 tccttacttg tcatgtttgc atttttcaca tattctacat ggtcttggaa ttgcagacgc
     7981 aaaaattgat ccgactcatc taatactttg acttggacac ctttttggat ggcatcgaac
     8041 ataaatatct gagtagaaag ttccatttcg cgaaaaccgg ccaactgata gggacgttca
     8101 tggctttctt tataatattg atgtcccaat gccacagcaa cttcttgctg actcgatagc
     8161 tgtgcttctt gatagagttt cgctgctaac gtttgttcag gatgatccat ttgagtcaat
     8221 gcttcctcta ctaaataaag agaatccaac caatccaatt caaccagcat ttgcttcatt
     8281 ccttcaagaa tttctctacc ttcttgatga aattctgttt gatctgatgg ttgttctagt
     8341 gctactttgt tatttcgagt cgtaccttct gctacccatt ggtcacagtc ttctttttct
     8401 tctgtccata acatgtacat caggaataat tgtaagaacc gaacttgctc tggattgata
     8461 cccaatcttg cataaggatt caaatcgata ttccgcaatt caatataacg aacacctgtt
     8521 gttgctaaat ctgcgacttt ctttccgcct cggaaacgaa gcggtgtata aaattctttc
     8581 tcctccgaaa gtttcccttc ttcgatcatt cgctcgatat ctgctaaata ctgtttcata
     8641 gaagcatagg aaactttcac atttggatga ttcgtatagc ccagcgcact gttgcgaata
     8701 ctgcgaacag gttcttgcgg atgtgattca tctaaaaagt agttcttctc actcattggt
     8761 gaagcaccga ataaataagt gatcatccag cgatagcgca taaagtttct cgctgttttc
     8821 aaataaagtt ctgttttgaa ttcggaaaaa tcttgaaatt cttcctgatg gctgaacaat
     8881 gttcttagca actcatcgcc aaattcaaaa ttgaaatgaa taccgcttac catctgtttt
     8941 cgcttcccgt attcttttgc taaataacgt cgatacaaga catcttcgaa attttttaat
     9001 tttgcaataa tgatctcttc gtctttttcc ggtaaggcag gtggcatact taatggccag
     9061 atcatctctt ctttcggtat agaacgagca gtcacatcat aaacagcagc gagatactga
     9121 aataattcgg gaatagaatc ggtaacaggg gtgatcatct ctgtttgtgt ttcgctaaaa
     9181 tctgtttgga tataggggtg ataggatcga tcgccaaaga ttgctggatg atcggttttt
     9241 gctagatttc ctgcaagatc aacccgctgt ccttcccgtt ctataccaaa tcgagcttgg
     9301 tcgataaaag ggcgcgcatt gacatgcaat aataattgct taaaattcat cattaagtga
     9361 cctccaattc atatgaatct aattataatc aaaaagtcta agaaaaagaa cgaaattgtt
     9421 tgagaaccgc ttacaaataa gtataaaaat gaaggcatca aaaacacagc tatgttaaaa
     9481 acaatcattc aaacaaggag ctgcaacttt tgtcacagct cctgcttttt agacatgggt
     9541 tattcttctt ttagtaatcc tttgaacaat ccaacgacaa aatcatttgg atcaaacggc
     9601 tccaagtcat cgattccttc accaagaccg accaatttca ctggtaaatg caattcatta
     9661 cggatagcaa gtacgatacc gccctttgct gtaccatcta attttgtcaa aacaagaccg
     9721 gtcacatctg ttgtttcttt gaattgtttt gcttgaacca tcgcgttttg accagtggtt
     9781 gcatctaata caagaagtgt ttcttggggt gcttctggta attcccgctg gattactcgt
     9841 tttatttttt ctaattcgtt catcaagttc actttatttt gcagtcggcc tgcagtatca
     9901 actagtaaaa catctgcctg ctctgtcttt gctctttcca aagcatcgaa tacgacagct
     9961 gctggatcgc ctcctgcatt gccacgaaca acttcaacac cagcgcgttc gccccagaca
    10021 actaactggt cgattgctcc agcgcggaag gtatctgctg ctgccatcaa tactttttta
    10081 ccttcgtttt tgaactgatg ggcaagttta ccaatacttg ttgttttccc tacaccatta
    10141 acaccgacaa acaaaataac tgttagtccg tcttcttgga tatttagttc atttacttca
    10201 ttgattcctt ccgcttcata gagatccaca agtttctcga tgatcgcatt ttggacttct
    10261 gccgattttt tcgcatttct gagtttcact tcttgacgta atgcttcggt aattcgtatc
    10321 gctgtttcaa aacctacatc tgcaccgata agtgtttctt ctaattcttc aaaaaagtct
    10381 tcatcgactg ttcgaaaatt ggcaaacaac tcattcatcc gttcaccaaa tgttttacgc
    10441 gtcttttcta aacccttgtc gtacttctcc tgtacttcaa cttcttctgt tttctcttcg
    10501 ccagtaaaag cacgcttgat tttatcaaaa aatcccattt gactccccct actctaatac
    10561 aaatttttgg atcactttgg ctacgccatt ttcttcattg ctggctgtca cgaaatctgc
    10621 tgcttctttg acgaaaggca cagcattctc catcgctacg cctagtccag catattggat
    10681 catcggtaaa tcattttcct catcaccgat cgccatgatt tcttcttgtt tcaaaccaag
    10741 atcttttgcc aataaagaaa taccataagc ttttgtaaca ccttttggca taaattccaa
    10801 aagcatactt cttgttttga tgatctcata catttcatgg aattctgctg gtatttcttt
    10861 gattttagga tctaattctt cttggtcata agcgattact gccttattaa gcacaaattc
    10921 atctgtaata tcgcttatat ccgctggttg gaattgcagc agcttattca acacattgta
    10981 caaggattgc ttccttgggg cactaggcag ctgtaaaacc acattgtcag acaaaacatc
    11041 tagtggcaga tccaattgct gagccagttt gtataagcgt tggatatctg atacttccat
    11101 cactttcttt tccatgattt caccagtatc atttttttgt accaaaccgc cgttgaaagt
    11161 gatgctgtag tcaccttcat ctaccagtcc taattcttgc agataatgcg ccatcgcagc
    11221 taaggggcgt cctgtacata gaacgatctt gaccccttgc tgttttgctt gtgctaatgc
    11281 ttgtttattc tcatctgaaa tcagcttttg ttcatttaat aatgtaccgt ccaaatcgat
    11341 tgctactaat ttgatcattt tcgttcctac actttcgttt cgttgatttt cgcctcttca
    11401 ttgatatctt ccatccgtac agaaacgatt tttgatacac cggattcctg catcgtcact
    11461 ccgtacagta catctgctgc gaccatcgtt cctttgcggt gtgtgactac gataaattgt
    11521 gtgtcatctt ggaaatcgct caagtaacga ccaaaacgtt tcacgttggc ttcatctaac
    11581 gctgcttcta cctcatctaa gatacaaaac ggaacaggac agacacggat aatggaaaag
    11641 agcaacgcga tggctgtaag tgctcgttct cctccagaaa gcaaacttag actttgcagt
    11701 tttttgccag gaggttgtac ttcaatctca atacctgttt tcagcaaatc agttggttcc
    11761 gtcagtacta attcagcttt accgccgcca aacatatttg gaaagacaat tttgaattct
    11821 tgacggatag cttcaaatac ttctttgaat cttgtccgca cttcatcatc catctcatcc
    11881 atcgtttcaa acaattggtt tttcgctgct aacagatcat ctcgctgtgt tgctaagaat
    11941 gtatgtcttt cactgacttg ttcgtattgt tcgattgaat ttaagtttac tggtcccagt
    12001 ttttcaatct gttcttttaa ggatgaaacc tttgttctac tgtcttcgat atcggttgtt
    12061 tcttgatagt cttgggaagc tttctcaaat gtcagctgat attctgactg cagataggtc
    12121 aaatgattat ctaaaatcat ttcagctcgg tctttttgga cttctaattt ggattgttcc
    12181 gacagtcgtg ctttttgctc acgattacgc tctgccaaga ctgcttcaag cttatcgatc
    12241 tccttttgcc ttctatctcg ctgctctttg gcttttgcca actcggcttt taatgtttct
    12301 cgctgagcag ataattcgtt aatttgtttt tccaaacttt cttctgtgac ttcatgatca
    12361 gaaaagtctg ctgtcaatgt cgccaactgc ttttctatcg cttcttgacg ttcggttgcc
    12421 tctgcttttt ggactcgggc accgcggagc tggatctgca gatgattaaa ctgctctttc
    12481 aagacagcaa gatctgcttg ttcttgtgct ttttgacttt gtacttgtgc tcttctggct
    12541 tccatctggt cgctttcttg acttaacgat ttgatttctt cgtcgatttt ttgtcgttgc
    12601 gattcaagat ctgtttgttt gtctttcaat tcgttcgttt gcttttcgta atcttcgata
    12661 aactgctgca gttcacgtgt ctcaaaagtt gagatttgct tttctttttc aaaacgttca
    12721 agttcattta tgatgttttg tagctgattt gtcgcttctt gttcttcaaa acgtaattgt
    12781 tcgcctcgtg tacgcaaaac ttcttgttct tcggctaaac gagctgtttc ttgttgcagc
    12841 tcctgaactt ttttttcttg tgcttggagt tgtttatctg cttcttcaaa ttcactagtc
    12901 aactgtttca attcttgatt ttgtacaaac aaactgcctt ggtttcctcg tttattcgct
    12961 ccacctgtca tggatcctcc ggcattcatc acatcacctt ctaaggaaac cactcgatat
    13021 tgatagcgga tcgtttgcgc gattgcattg gcactagtta agtctttggc taaaaggatc
    13081 gttcctagca ggttttgaac cacagtttgg atttgatcgg gataactgac ttgttcactg
    13141 gcaatcccta taaacccttc tacagctgct gcttgtgtca atatatgagc cggtaattgt
    13201 cttggtttga ttgtcgtcag tggaaggaat gttgctcttc ctcctctttg ctgtttcaaa
    13261 tacgtgatgg cttgccgagc atctttttca ttttcaacga tgacatgctg ggctgcccca
    13321 cctaaagctg tttcgatagc taacgtgaaa tctgcaggaa catcgatcag ttcagccact
    13381 gctccgacaa tacccgaaag ctgctgttta tgctgcaaga ccaatcgaac cccttggtaa
    13441 aaaccaaaat agttttcttg gatttcttga aggcttttct tacgagcacg tagctgctgg
    13501 acttgattca ttaattgata cattttaggc tgttcttttg ctaatgcttc ttggaacttt
    13561 ttcccgtttt gttggatcat ttctaacttc tcttttgttt cagttaaaga tgattttagc
    13621 tgcgcctgct tttgtgtcaa ttcttctttt tgtaagatca atcggtctac tgatgcttct
    13681 acttccgatt gcttggctaa cgtctgcttg ctttttgctg tttcttgtat atattgacgt
    13741 tctagatact tcagttcgtt tcctacagct gcttgttctt gcatcaaatc aacatattga
    13801 tctcttaatt ctgccaataa ttctttcgtt gatttttggt atttttctaa ttcttgctgt
    13861 gtttttatga gatttgcttc agctttttgg atctctgttt ctttttctgc tgctgctttc
    13921 atcaagcttt cttgaagctt ttcaaaatgt ttaacctttt tttgtgcttc tgctaatgaa
    13981 gtttgatatt cttgcgagct tttttgtgta tgctttgtcc gctcctgcaa aacatctttt
    14041 tgtccttctg tctgcttcag tttttccgaa agatctaata atacttgctg atttttttcg
    14101 atcagacgat cggcttgcgc attttcttta cgctgcttcg ccaaaatgga ttcttgttct
    14161 tggatcgact cagatagttt gcctaattct agattgaatt tcgcaagctg tgcttgtttg
    14221 ttgtcccaat ctttttttgc tgtcttgatt tcagcaacca tcaaagagac atcggtttga
    14281 gtcagtgttt cttttagccg taaaaactcc tttgctgcct cactctgggc ggccaaagga
    14341 gttaactgtt cttccaattc gtggatgatg tcttggacgc ggctgaggtt gtcttctgtt
    14401 tcaaaaagct tctgttctgc tttctttttc cgctgcttgt atttcaagac accagctgct
    14461 tcttcaaaga tcccacgacg gtcttccggt ttactactga aaatcgcttc tacttttcct
    14521 tgagaaatga ttgagaacga ttcttttcct aatccagaat ctaagaacaa ttcttggata
    14581 tcttttaatc tgcagctttg tttattgatg aaaaactcgc tttccccagt ccgtcgataa
    14641 cggcgggtta cactgatctc attgtactcc aatggcagat aatgatcact attgtctaaa
    14701 atcactgtga cttctgcaat attcagctgc ttacgggtat cggaaccggc aaagatgata
    14761 tccggcattt ttccgcctcg cagactttta gcagattgtt ctcctaatac ccagcggata
    14821 gcctctgtaa tattgctttt tccgctgcca tttggtccaa ccactgctgt gacgctattt
    14881 tcaaaatcaa tgactgtctt gtctgcaaat gatttaaaac cggctatttc aattcgtttc
    14941 aaatacaaag ctttccctca cttactttta cagtgctgcc aatgcatttt ccgccgcagc
    15001 ttgttctgct aattttttcg atttcccttg tccagtgcca atcaattgat catctacata
    15061 aacttcgatc caaaatactc gttcatgagc tggaccttct tcattgatca atcggtattc
    15121 gattgataca tcacctgatt tttgcaagac ttcttgtagt tttgttttat gatccatctc
    15181 atgtgaaaaa gcacctgctt tgactttagg aaaaatcact ttttcgatga atgcatgggt
    15241 cgtgtcgaat ccctggtcaa gatataacgc gcctaaaaaa gcttcgaata ggtcgcaaag
    15301 caatgctggt cgcgtacgtc ctcctgaatt ctcttctccc ttgcccaaca aaacatattg
    15361 atcaaagtga cattctttgg caaatttcga caagctgtct tcgcgaacga tcgcagctcg
    15421 tgtttttgtc aattttcctt ctggcaaatg aggatataaa cggaataaat actgagaaac
    15481 catcagttct aaaacggcat ctcctaaaaa ttcaagtcgc tcattatccg ataattgcag
    15541 attgcgatgc tcattcacat aggatgaatg ggtaaaagct tgttcaagca aatttagatc
    15601 gtgaaaaacg atgttatagt tttcttttag ttcttttgtc agctggttgt ccattttcca
    15661 cattcctttt atggatattt tttcgatact tccttattat actggttttt tgtcaaattt
    15721 gaaatggttc ggtcaaaagg taagtaaagc tttaataatc ctagtgattg acgagaagtt
    15781 gtgaaaaatt cgtttagttt cgagtatgaa gaaaaaattt tgtgaaaatg gctttccata
    15841 tttttgcata aatttgactt aataccgaga aactggactt tgaacaccgt ttattggaga
    15901 agtcatgaaa tcagcgcctt gacctgaata ttagagaaat aaacagaaaa atatcaaaaa
    15961 accgaaaaag gacatcgccc tttttcggtc atttttagtg attatgaacg tgaatctgct
    16021 gtaacagaaa catttgccca tgggtttggt acatcataag accaatccca gtctttgaca
    16081 cggtcgttga ctggcaatac ttcattacgg aacaacgttg gaatcgcaaa tgcttcttca
    16141 aatgcatatt gttgccattt ttgatatgct tctttttggt actcttcatc aaatgctttg
    16201 cttgaactga tatcgttgat caattttgta ttttcttctg attcataacg agtgtagttg
    16261 aacgcagcat ttggtcccca tagtccactt ggatttggat cagttcctgt attccaagca
    16321 gcttggtaaa catcaatttc aggatcatcg ttttctaatt tatcatagaa agaattgaat
    16381 tcgatcagac gacctgttgt gtattggaca tccaaaccaa tttctttcca ttgttggata
    16441 tagtaatctg ccaatggttg cgctgtttcc ccaccggaca tcgaagcaaa gttgattgtt
    16501 agtttatttc catctttgtc ttctcttaaa ccatcgtcat ccacatcttt gtagccagca
    16561 tcatctaata gttttttcgc tttgtctaga tcttgtgtga atcctttcat ctcatcatca
    16621 tggtatgact ggaatactgg aggaatcaat gaagtcgcat tgcttctcaa acctgcatag
    16681 aaacgttcac ctactgcatt attatctaat gcatagccca ttgcttgacg taatgattta
    16741 tcagccattt ttgaatctgg attgtattcc acactctttg tatctgcatt ccatttacct
    16801 aacttgaatc ctagataagt ataagaagtt tgttcacggc ctaagtttgt atagccagca
    16861 atttctttat aggtatcgta agtatctgtt ggcatttgga aaatcatatc atataattta
    16921 gagttcaatg cttcagttgc acttgccatc ggtacagatt tcatcgtgat ctttttcaat
    16981 ttaggcgtac cattccagta gtgctcgttt ggcaagtatt ctactgattc tcctgcaacg
    17041 atattactca tgtaataagg gccaaaagtc actggatttt tacgtacttg atcgcttgat
    17101 tccatatctt tgattgctac accttctaac gtatgttttg gtgctgcata tgaccagata
    17161 ccaccacccg attgaagcat gcttgggttt acttctttat attcgatttc aactgttttg
    17221 tcgtctattt ttttgattcc ggagattgtg tcggcacttc catcatgata ttcttccata
    17281 cctacgatgt tacggaaagt gtcgtcataa cggataccag tgtaatcttt attcccaatg
    17341 atttcgtaag ggaaaatcac atcgtccgcc gttacatctt caccatctga ccatttgacg
    17401 ttgtctttta ctttgattgt cgcttttttc gtatcttcat ctagatcaag cgttgcagct
    17461 ccgtcgtctg tgattctgaa atcgtcatcg tttgcaaata gcggttcatg agaaggagcc
    17521 ataaaagctg catcatatga atcttggtaa aactcccact ggaacaatcc tttaaactga
    17581 ctgtccgttg cgaccgcagc ttctaattcg ccgtcttcga ttgcatcatc attatttttt
    17641 gttgcaattg gaaaagttaa ttcctctttg ttattgccgt tgcctccaga gttgtttcca
    17701 gaggtatctg tacctccgcc cccgcatgct gcaagaacga cagcagacat cagcgctgca
    17761 aatccaaaaa tcgttttctt cattcctatt tcccccttcg cttctatact agcctaaccg
    17821 ttggcgcgca tctgcagaac gcttgaatgc ttgtccaaca taattgatac tcaacatcaa
    17881 aactaaaata aggattgatg cgggtaacca gatccattgc cgattcacta agacttctcc
    17941 gttacttgca taaccgatca atgtacctaa actaggtaca tttgccggca agccgaaacc
    18001 taagaaagtc aaagttgtct cgataccgat gtttgccgca aagttcatcg tcaaattagt
    18061 aatgattaat gaacttagat tcggcatgat ttcgcggaac atgattttaa aatcattggt
    18121 tcccattgtt tttgaggcac tgacataatc tcttcttgct tcagataatg tcttgcttcg
    18181 gaataatcgg gctttagcta cccaataaaa tgcactcatg atgaatataa aggaccagac
    18241 attgtatctg gaaataatcg atacgaagac gataatgatc agcatgatcg gtagaatcat
    18301 gataaaatca acgatccgca tcaagatgtt gtcaatgatc cctccgtagt agccagagat
    18361 gatccctaat ccaaccccga tgatcgaagt aaggatcgtg atcgcaaatc cgatcgtaac
    18421 agagtttcta gcacctatga tcaattgtcc taatacatca cgtccgcctt catctgctcc
    18481 cagaatgaac ttttgtcctt gtgtcgaaac tgttcctggt gcagcgtatt tgtcgaagat
    18541 actaacatac atcactttgt cttgatcggt cagcatcgca ccgataaaga tcactaataa
    18601 taaaaccact agcaaaccta atgaaaaaat agcaatttta tcttttttaa attctcgagc
    18661 aatcattcgt atccccatag gaggaatact ttcttgaact gctgtattgt ttttttcact
    18721 catcgatcca ttctcctttc tgattggttt tattgaatcc gaatacgcgg gtcaacgata
    18781 ctcatgatga tatctgaaag taatgtcccc actagcgtcg cgattcctaa aatcaatact
    18841 aaggatgtga tcacggtata gtcccgttgg ccgatggaat caataaatag ttttccgact
    18901 cccggataac caaaaatctt ttcgataact acagaaccag caatcaacga agtgatttca
    18961 taccccattt gagaagccat cggcaacgct gcatttcgga aaatatgtcg actgaatact
    19021 ttattcgtcg gtaccccttt ggaacgggct gttctgacaa aatcgagcga tttcgaatcg
    19081 attacttcac ttcgcagata ttggattgtg acggcagtcg ccaaaaatgc ttgggtaatt
    19141 gatggtaaca gaatatattt caaacggtct atccattgag tgataaatcc accttgcaaa
    19201 cctagtgttt gtgatccact tgttgggaac cagcctaaac gataaccaaa aacaaatagc
    19261 atgatcaagc cgaagataaa taaaggaaca gcaaaactaa agaagttgta gattacgaca
    19321 atcttatcca agattgaatt ttgataccgg ccagcaagca tccccagagg cacagcaatc
    19381 aaataagtaa ggacaactgt caagatcgat agatataaag tgttgatgat ccgttcgccg
    19441 atcaagtcag caacagagcg tttgtataag aaactttgac caaaatcgcc ttgcaaagca
    19501 tttccgatcc aacggaagta ctggacatac catgggtcat tcaagccagc tgcttgtcgc
    19561 atctgttcga tcactgccgg atcctgattt ggattgatca aaccggtaaa tggatccccc
    19621 ggcatcgctt gggctaataa gaagatcaga acacttaaaa taataacttg cgggatcatc
    19681 aataaaacac gtcgcagaat cgttttccac attagcttgc acctccatct ttcaacgcca
    19741 ctttgtgtgt agttgtgatc gtacgcaaat cgtagactcg accagtcgca tcatagtatt
    19801 ctttttggtt ttggatatac tcttgttcta ctcttcgtcg attttctttg tggctttccc
    19861 tattttctac atcgatttgc ggaatagctg aaagcagacg tttcgtatag atatgtcgcg
    19921 ggtcattata aatgtcttct cttgtaccga tttctacaaa tcgacctttg tacataattg
    19981 cgatattgtc gcacatgtgt tttactacac ctaaatcatg tgaaatgaat aaatagctga
    20041 ggccgtattc ttgctggatt cgtttcatga aattcaatac ttgcgcttga acagatagat
    20101 ccagagcaga tacaggttca tcggctacga tcagttttgg attggtagca acagcacggg
    20161 ctactcctag acgttgacgt tgtccgcctg aaaattcatg aggatattta tatagcgcat
    20221 cttccggcat cccaacgata tctagcagtc cttttacctt tttcttttct tcttgatcgc
    20281 ttaaccgttc aaaatttcgg atgggttcag caatgatatc caacactcgc tttttaggat
    20341 tcaaactgga cattgaatct tggaagatca tctgcacatc cttgttataa cccatttttt
    20401 tgcggttgct tcgcttcgtt acatcttttc cttcatacat gatttgtccg gctgttacct
    20461 tttctaatcc cacaacggct tttcccgtag tagattttcc agagccagac tctcccacta
    20521 aaccatatgt ctttcctttt tcgataataa aatccacacc atctacagca tatacatgat
    20581 ccgttactcg attgaagaat ccactgcgaa tagggtaatg aactttcagg tctttgattg
    20641 tgattaattc tgtcatctac acctctcctt tttcgtctct aaagtggaaa tgcttatagc
    20701 aggagcaacg taccaagtgg ttcggtgcga cttcatgaag gataggctct tcttcatgat
    20761 cgcttgctgg tatccatgga atacgtggtg caaaccgaca acctttacga ggcatgtttt
    20821 tcagagaagg aacgacacct tcgatcacat ggagttctgc atcatgattt ccttcttgtg
    20881 gtatagagtt cagtaaagag cgtgtataag gatgcttagg atgatcaaac agttctttta
    20941 cacctgctac ttcaacgaac tgtccgccat acatcactgc cactcgatca gccatttctg
    21001 ctacgactcc taagtcatga gtgatcaaga tgatccctgt ttttgtttct tcctgaaggt
    21061 ctttcagtaa atcaagaatc tgtgcttgga tggttacatc tagggctgtc gtcggttcat
    21121 ctgcaatgac gattggaggt ttgcaggcga ttgcgattgc gatgatgaca cgttgtctca
    21181 taccgcctga tagttcatgg ggatattggc gtccaactct cgcaggatta ggaatcccta
    21241 cttgttctag caattctagt gcacgagctt gacgctgttc tgcattcatc tttgtgtgat
    21301 aggaaagtcc ttctttgatc tggtcttcga ttctcatgag aggatttaat gcagacaacg
    21361 gatcttggaa aatcattccg atatcatttc cgcggatttt attatagagc gtttcgttca
    21421 ggtcgattaa attcagatcg ttatataaaa tctcacctgt aatttttgtg ttattaggat
    21481 caagtaatcc cataatcgta gtagccaagg tacttttccc gcacccagat tccccaacga
    21541 ttgctaaaat ctcgtttttg tctaaagtaa atgaaacgtc atctactgca tcgtaatagt
    21601 catctttgag tcggaatcct gtatgtaggt tctgaacttc taataattgg ttatcggaca
    21661 atcgaatacc cctctctgct tcctaaatgg aagtttttct taataaaaaa ggacattaca
    21721 cagaataaat cgttttataa caaaggttat tcttatcttt gtgtaatgaa ttattaataa
    21781 ttatatagaa aaccgcttta ctttgcaaca taatttctgt ttatcgccag aattatcaga
    21841 aaaatgactt ttacgtgtca aaaagattaa aaaaagaagg ataaaaagct gcatgcagct
    21901 ttttatcctt ctttgctttt atttgaaatt atttaagatg gctgctgata taatctactg
    21961 cgccaccgac tgtttcaatt tgttcagcgt cttcatctga aatttccgta ccgaattcat
    22021 cttcaagctc taaaacgaat tccataatac taattgaatc agcttttaaa tcatctttga
    22081 tgttcaattc atccgtcact ttatcggtat ctatctcaaa atgatttgaa ataattttcg
    22141 ctactttgtt aaatacttct tcgcgagtca actttttcac ctccaagaat catacacttg
    22201 tcaattacct acaccatggc taattttact ggcatttttc aggaatgtcc accttttttt
    22261 caatggaacg gatcgcttca gatatccaaa tactattctt cgcttttttc aaattgttcg
    22321 actaattgtc caactacatc tgtctcaagc attgtgtgga tttggcggat cgtatagcga
    22381 acagcatcgg ggcctgttgc tccatgagtt ttaatgactg gtgctttcaa gccaaacaaa
    22441 acggcaccgc catgtttgga ataatccatt tctgctttca aagaacgtaa gccgtctttt
    22501 aaaagcatag cgcccatttt tcctttgatc ccttcattta gaatagcaga ttttaataga
    22561 ttcatcatat tcattgctgt tccttcaata gatttcaaaa cagcgttgcc tgtaaatcca
    22621 tctgtgacca ctacatcagc cacaccattg agtagctcac gagcttctac gtttccaata
    22681 aaattcaagc ttgtttcatt ctgaaggagt tcataggctt ttttcgtcac ttcactacct
    22741 tttgttgctt ctgttccgtt gtttaagagt gcgacacgag gacgtccgat acctcggact
    22801 ttacgtgcat aaaaagaacc taaaataccg tattgcaaca aatgttctgg tttgttttct
    22861 gcgttagcgc caagatccag catatcgaaa ccggcacctt ctttccctac tactggcaag
    22921 gtagacatca agccagggcg ttcgatattt ttgatacgtc ctacgatgaa caaccctgct
    22981 gctagtagcg cgcctgtatt ccctgctgaa aatacagcat ctgcttcacc gtttttcact
    23041 gcttgtgcag caaggaccat tgatgcagtc ttttttcgtc gaattgcttt tactggctca
    23101 tcatcactat tgattttttc gtctgtatgg ataatcgtga tgtttttttc atttgttaag
    23161 tatttcttga tttctgcttc ttttccgtaa agtaagaatt ctacttctgg aaaatcttgc
    23221 tgcgctaaag ccacgccttc tacaattgct tgaggtgcgt gatctccgcc cattgcatct
    23281 acggcaattt tcattgtttc cactcctttt tccgtcatca gtataaagta tagcacagtc
    23341 tgctaatcga aataacgatt atcctgattt tctatttgga ggctttgcgc taaaggctga
    23401 tactcaggaa gcatctgcca atcctttatt tgccaaaggg ctgcagcctc ttctctggct
    23461 acctccaaaa catttccatc cgtcaccaag tcagcaatcg caaattgagg cagaccagat
    23521 tgacggaagc caaatacttc accaggacca cgtaattcca gatctttttc gcttagtaca
    23581 aaaccattgg tggtctctgt catgattttc atccgttcca cacctagttc atttttagga
    23641 ttagcaacta agatacagta ggaagcatca cttccgcgtc caacacgtcc acgaagttgg
    23701 tgcagctgtg ccaatccaaa tcgatccgca tccatgatca gcatgacggt cgcgttcggc
    23761 acgtttaccc ctacttcaat tacagtagtc gaaactaata tctgcatctg gttgtctttg
    23821 aaggcttcca tgattacttc tttttcctga ttcttcattt taccatgtaa caagccaact
    23881 tcatattttg gtgcaaatag ttcacgaagt ttttcataga tttccactgc attttttaca
    23941 tctaatgctt ctgattcttc aatcagcaga cagatgacat acatttgatg tcctcttgcc
    24001 agttcttttt tcgtccaatc cagtacactg tccaattgag gtgttcttac ccagcgtgtt
    24061 tcgatcggga tacgtccagc tggaagttca tcaatgatag atacatccat ctcaccatat
    24121 gctgtaatag ctaatgttct aggaatcgga gtcgctgtca taaataagac atctggtcgc
    24181 cagccttttt cacgaagtac tttccgctgg ttcacgccaa aacggtgctg ctcatccgta
    24241 ataactagac ctaaattctg aaattcgact ccatcttgga tcaatgcatg tgtgccgatc
    24301 acgacgttaa tttccccttg ctctagtttt tcaaggagct cacgtcgttc ttttgctttc
    24361 gttgaccccg taatcaatgc gatggttact tcattgggat caaacagctg ttgcaagctt
    24421 tccatatgtt gttgtgccaa gatttctgta ggcaccatca acgcaccttg gaaacctgct
    24481 gtaacggtag cgtataacgc aatcgcggca accactgttt ttccgctacc tacatctccc
    24541 tgcaaaagtc gctgcatatg ctgcggactg cgaagatccc gacagatttc attcgttaca
    24601 cgtttttgtg catttgtcag ttcaaaaggt agcttttgag taaattgttt caatttttgg
    24661 acatcataaa ggatctccaa accattcgtt tctgcttttt ctgctttttt caatccttga
    24721 agacgcattt gaaataaaaa gaactcttca aaaacgacac gtcgtttggc ttgatggttt
    24781 tcttcgtggt cttttggaaa atgcatactt cttacagcag aagctcggtc aagcagtcga
    24841 tatttctcca caaggttgtt tggcagattt tcttcaatca atgtgccgta ttcttgaaac
    24901 ccttttttga ttagatcaac taatgtcgtt tgacgaattg atttgttgac gtgatatatc
    24961 ggcgaaaagt catccattgt ttgagaacct aaaatcttca tcccgttcaa ggctttacgc
    25021 tttgcatccc atttaccgta tacggcgatt tcctccgata aaatgacttt ttcttttaga
    25081 taaggttgat taaaaaaaga aacgttgaac acatcatgtt cttgcatcat acgaaattgc
    25141 aatcggcttt ttttataacc aaatcgattc atcacaggag gcgagactac gattcctttc
    25201 aaagtaacct tttcttgatc ctggatctcg tttagatttc tttcctgaat atcttcgtag
    25261 cgaaaaggat aataagtcag taagtcttca atcgtttgga tgcccaagct ggcaagacta
    25321 gctgctcttt tttctccgac tcctgccagc atagtgattg gatcatctaa agaacgcata
    25381 aatttttcct ccctccttta a
//
LOCUS       DACDXO010000036        24666 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E23605_2017
            SAMN12639989-rid9881463.denovo.036, whole genome shotgun sequence.
ACCESSION   DACDXO010000036 DACDXO010000000
VERSION     DACDXO010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639989
            Sequence Read Archive: SRR10023389
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24666)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24666)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741914.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:42:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,972
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 33 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 17 of 73
            Pseudo Genes (multiple problems)  :: 10 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24666
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639989-rid9881463.denovo.036"
                     /isolate="E23605_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(368..1486)
                     /locus_tag="IWM35_001661"
     CDS             complement(368..1486)
                     /locus_tag="IWM35_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9919517.1"
                     /translation="MAKKDKELLTQLTSEGAKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKIENLLGDPSFWRSATTSVAVIITADQTYIHRLSI
                     PVDDQYYVSDLPYLLAIIKNGQFNYSYYLLGLNRDSMKLYEVSNKQVKEVSLPEGAPV
                     DVVTALGSELTGGGLNFSSNGGGGARDGVTFHGVNPKDEEVEIDWVNYYQAVDTYLKD
                     SFENPEQWPIYLFALPENQTMFKKIAKNPYYCADVAISASPAQLSFRDIEEGAKKLSS
                     ELAAQESKEYNKLLDKKFIDQLVDIVPAAKEGKVSHLFIATSNLADGFGEDPETEYDR
                     RQVLNALADDVIINGGKVFILDQKDAPDEKSLTAILRY"
     gene            1957..3264
                     /locus_tag="IWM35_001662"
     CDS             1957..3264
                     /locus_tag="IWM35_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HAP9919518.1"
                     /translation="MSIKKTRLVLLLASLILCLASFLPAVAVQAEDTFKVNAKAAFAV
                     DAQSGKILYDQDGEKTMGIASITKIIGLYIVLDQVKEGKLSWGDKVSISDYAENLSIT
                     PDLSNVPLHKENTYTVKELFDSAIIQSANASMVALAEKISGSEAKFTERMKEQLKDWG
                     IKDATIVNASGLNNSYLGENRPEGTGENDENQMSAQDVAIVARHLILDFPEILDVSST
                     TTQMFGENTQSPVEMVNWNWMLPGFVNYKEGVDGLKTGTTELAGACFVGTITKNDQRI
                     ITVVLNADGHAENPSARFNETDRLMDYCYDNWSEKELGKANASIPSLKTIDVKDGKES
                     TVPVVLKSPVKVWVRNDMDTGKLTITPTLDKKQVTDNEIQAPMDKGTKIGTATITLAD
                     DKLGYLEDNNAPSTEIITDKTVEKANIFVIGWRRVAEFFGNLF"
     gene            3383..4582
                     /locus_tag="IWM35_001663"
     CDS             3383..4582
                     /locus_tag="IWM35_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HAP9919519.1"
                     /translation="MNLTRDLIFLVLYLIGVFSIVYWRKQKKDPSLFFYGSTWTFTVI
                     LTYFVVLEIFYSTFYFLIPLLFFSIFAFSYFTEKRRLLNGLLFNVLLISFGIYLFVLL
                     YETQNIFLGGLIALITIPLLLVLLFGIYGLIVFLFWNGVTVLRRESHSLANLLTLILA
                     IFLTLFLVFDFFLLKYLPQWINALFFCVPLILIYLFIVFYNFLTVSFLYQFNRPRYNQ
                     DFIVVLGAGLINGETVSPLLAKRINKAIAFYRAQSRATLNPPILLMSGGQGADEKVPE
                     AIAMKQYAMEQGIPERDILVETNSTTTLENMLYSKEIMDQQMKGPYRAIFSSNNYHIF
                     RAGLYARQAKLKANGIGAKTAFYYLPNAFLREYLAILMMNKKRHMIICGLLFAGSAFL
                     SLITLLF"
     gene            complement(4609..5877)
                     /locus_tag="IWM35_001664"
     CDS             complement(4609..5877)
                     /locus_tag="IWM35_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HAP9919520.1"
                     /translation="MEKQLPSPVRILVYMLKWLVITAILGIFMGSLSAFFLNSLTVVT
                     DIRLSHPWLLCLLPVSGALFAYLYAYHGGLSSRGNNLVIDQGNGGEEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALADNTARLFRLDKSEREILIISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEAIFPSFFAALFANFMTESYGVSHLHYQMGVIPEWSML
                     LFFKVFVAGIAFGLIGWVFSRSIVFLKARYAQWIPHPVCRNTIGGAVVVAAALILQTQ
                     RYLGLSLPLLQESFNGQAHVYDFIGKLFFTVLSLGAGYQGGEVTPLFEIGATLGAALA
                     PLLHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGSQAAVYFFMICLISFMCSGN
                     SGIYSAQQVAVKKGILFLPIVKRMKEKNKV"
     gene            complement(6040..6686)
                     /locus_tag="IWM35_001665"
                     /pseudo
     CDS             complement(6040..6686)
                     /locus_tag="IWM35_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728800.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FusB/FusC family EF-G-binding protein"
     gene            complement(7094..7597)
                     /locus_tag="IWM35_001666"
     CDS             complement(7094..7597)
                     /locus_tag="IWM35_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP9919521.1"
                     /translation="MTEKWREDEEYMSYVQDLLETEAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYLLAKKWGGNAKATARAGLLHDLFYYDWRTTKFDEGTHAYIHPRIAVKNAEKI
                     TDLSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMSASMRKRWQRY
                     FGKESSI"
     gene            complement(7720..8484)
                     /locus_tag="IWM35_001667"
     CDS             complement(7720..8484)
                     /locus_tag="IWM35_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HAP9919522.1"
                     /translation="MKEIQSVKNPRIKELKKLHKKKYREEKAEYLLEGFHLIEEAASS
                     DAEIKELFVTQRGMKEWGDWLAVNKEISTFLVSDEVMTAMSDLPTPQGILAVMTLTSE
                     DTPDFSGGWLLLDNVQDPGNVGTMIRTADAFGMAGVLLGKGTADIYSTKVLRSMQGSN
                     YHLPVLQKDLAEVIPVMKGKGFTVYGTELNEEAHALDKVEKTEDFAIIMGNEGQGVSQ
                     EILSSTDQNVYIPMKGNAESLNVGVAAGIVLYHFSL"
     gene            8591..8866
                     /locus_tag="IWM35_001668"
     CDS             8591..8866
                     /locus_tag="IWM35_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HAP9919523.1"
                     /translation="MRKVKMNVQGRVQGVGFRYMTKMVADQLGVTGTVKNEDDGSVTI
                     EAIGNDDIIQKFIEEVKKSPSPSGRVQYVDVQEDPMIEERKKFDVVG"
     gene            9582..10535
                     /gene="yidC"
                     /locus_tag="IWM35_001669"
     CDS             9582..10535
                     /gene="yidC"
                     /locus_tag="IWM35_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HAP9919524.1"
                     /translation="MNKWKNWLLGSGLVSILLFLSGCVRMDSNGNPDTSGIVYRVLVH
                     PMGQAITYLVENFNWSYGWAVIAMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     AAQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESSFYGIDLGSPSMVLVAIAGGAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGVFSCLQTFITNVIMKPRLKAQVAEELKQNPPKQVVTPR
                     KDVTESAAKPADNKQATKQIKQPKNSTNNKGRNAGRQQKRK"
     gene            10657..11866
                     /locus_tag="IWM35_001670"
                     /pseudo
     CDS             10657..11866
                     /locus_tag="IWM35_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017466553.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182 family transposase"
     gene            complement(12176..12397)
                     /locus_tag="IWM35_001671"
     CDS             complement(12176..12397)
                     /locus_tag="IWM35_001671"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP9919525.1"
                     /translation="MFKNYTINQKILPLDMEQYISETDVAFAVNALVEEKIKVDLYVS
                     SKFTNVKVVGADLFVVNVRKQKVSETDKF"
     gene            complement(12558..13079)
                     /gene="tnpA"
                     /locus_tag="IWM35_001672"
                     /pseudo
     CDS             complement(12558..13079)
                     /gene="tnpA"
                     /locus_tag="IWM35_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631987.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(13170..13536)
                     /locus_tag="IWM35_001673"
                     /pseudo
     CDS             complement(13170..13536)
                     /locus_tag="IWM35_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875519.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
     gene            complement(13550..13835)
                     /locus_tag="IWM35_001674"
                     /pseudo
     CDS             complement(13550..13835)
                     /locus_tag="IWM35_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311319.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
     gene            complement(13841..14035)
                     /locus_tag="IWM35_001675"
     CDS             complement(13841..14035)
                     /locus_tag="IWM35_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9919526.1"
                     /translation="MAETRPKGYPKLKEYTPSRFMLSECHYDKARADRAVNFIGQLRH
                     TKGKWAGNRFWLLPWQEQII"
     gene            complement(14035..14511)
                     /locus_tag="IWM35_001676"
                     /pseudo
     CDS             complement(14035..14511)
                     /locus_tag="IWM35_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004070864.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
     gene            complement(14979..15332)
                     /locus_tag="IWM35_001677"
     CDS             complement(14979..15332)
                     /locus_tag="IWM35_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9919527.1"
                     /translation="MAMSEKAKKYLKEIKGAKTIPELKDVEIAIKRDGILAWAEFTKL
                     NEAVEEKKVALRKKKQETSLQEILFWAYKKESDKLLKMMDEGADKDAIQMQVQRYDSI
                     GQIIAEADLEDEYEV"
     gene            complement(15460..15999)
                     /locus_tag="IWM35_001678"
     CDS             complement(15460..15999)
                     /locus_tag="IWM35_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9919528.1"
                     /translation="MQLLLYRIGDSTKIRFLINVRFRVIYSTVLPKGIPQRQKWMIHM
                     EEIMQMILGNAKFQLGQVVSTPAVLETFNPLQTANGIPEALSNLWFRQEISIAEIEDA
                     VLDAKNGKELANSLNRIKLFRKFTLDRELDGMVRLKGTDVWKNISYLKVYTATPPKKP
                     TPPRFQSNGWSLFFSHKSA"
     gene            16029..16220
                     /locus_tag="IWM35_001679"
     CDS             16029..16220
                     /locus_tag="IWM35_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9919529.1"
                     /translation="MIIRDDHIYTCDSCHYSFPADEQPERCPDCEKTATRLDTEIETE
                     DYYRVRAEIKAEIKALNAG"
     gene            complement(16262..17128)
                     /locus_tag="IWM35_001680"
     CDS             complement(16262..17128)
                     /locus_tag="IWM35_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP9919530.1"
                     /translation="MEELFSNALKSVKSNRVLYTASFFARSSLLYLQEIGQLTALKAH
                     TSRRTGLSSYLCFLVEVGSGTLEYKGQKYTLQVGDMVFIDCLKPYVHESSEDDLWTLR
                     WCHFNGIGMTEVYKKYLERGGQPVFKTSKTDKYADLLRSLHMVAEGQEYVRDMKINVL
                     LSELLMHLMEDSWNPDKVPNFSTKKMELQNIKQYLDDHYTEKITLDQLSAEFFINKQY
                     LAKIFRETYGTTVVGYLNQKRITQAKHLLRFTNLSMEEIATKIGITDANYFSRTFFKV
                     EGVRPSEFRKMW"
     gene            complement(17152..18804)
                     /locus_tag="IWM35_001681"
     CDS             complement(17152..18804)
                     /locus_tag="IWM35_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HAP9919531.1"
                     /translation="MNYKVEYERWLNHANADIIEELRSYSEKDIEDSFYKNLSFGTGG
                     LRGTIGAGTNRMNVHTVGKASQGLADYLNKNYEAASVVVGYDSRIKSDVFAKTAADIF
                     AANGIQVHLWPELLPVPTVSFATRYLKADAGVMITASHNPSKYNGYKVYGADGCQITT
                     EAATAILAEIEKLDIFADVKMDGDASKIASIQSEVLTAFIEEVKGQSVLFGEEVDKNV
                     AIVYSPLNGTGLVPVTRTLAEMGYSNVTVVEEQRLPDGNFPTCPYPNPEIKEAMSLGM
                     EYAKKYNADLLLATDPDCDRVGIAVKSAHGDYVLLSGNETGLLLLDYICSQRIKHGKM
                     PADPVMVKTIVTMDLGERIASNYGVRTIDVLTGFKFIGEQIGMLEKAGKVDSYIFGFE
                     ESYGYLSGSYVRDKDGVNAAYLICEMFSYYKTQGISLLDKLNEIYTKYGYCLNTLHSF
                     EFDGAAGFTKMQEIMVEFHKGLDIIGGKKVIETLDYSKGLNGLPKSDVLKFMLEDNCS
                     VVVRPSGTEPKLKTYISVSAENKEAAEAMEAIMAAELAEMFE"
     gene            complement(18809..19711)
                     /locus_tag="IWM35_001682"
     CDS             complement(18809..19711)
                     /locus_tag="IWM35_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HAP9919532.1"
                     /translation="MMNLQKELRCLLLRKLGMLYAPGWTELCCVWSEYQGMTLTEVLQ
                     EHPEFLGTHPQTKGELPILIKFIDAKKDLSVQVHPTDEYAIEKENGQLGKTEMWYVLD
                     ASLIYGLQKITEEAVIREAIAQGTVERYLQKVPVKKNDVFFIEAGTIHAIGAGALIAE
                     IQENSNLTYRLYDYDRVDKNGKKRELHVDKALEVANLKGLSEPKQPLRVLKYRAGVAA
                     ELLCRCKHFEVSRMIVNTERRQTVHYRADSISFRVLLCVNGCGTVRFDNESLDIYKGD
                     CLFVPADSTEISIHGSIQFLDVRG"
     gene            complement(19795..21108)
                     /locus_tag="IWM35_001683"
     CDS             complement(19795..21108)
                     /locus_tag="IWM35_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HAP9919533.1"
                     /translation="MNIVLLSGGSGKRLWPLSNDIRSKQFIKIFKTEDGYESMVQRVY
                     RQIKKVDNDATVTIATSKTQVSAIHNQLGEDVGVSVEPCRRDTFPAIALATAYLVDVM
                     GIDPEESVVVCPVDPYVEDDYFEALKSLSDQADKGEANLVLMGIEPTYPSEKYGYIIP
                     ESAEQTAFVNTFKEKPSEAVAKEYIAQGALWNGGVFVYKLKYVMERAHQLIDFVDYQD
                     LFNKYDTLTKISFDYAVVEHEDKIQVQRFAGMWKDLGTWNTLTEGMEESIIGKGVMNE
                     NCDGVHILNELDVPVLAMGLHDVVICASREGILVSDKEQSSYIKPFVDKFEQQIMFAE
                     KSWGSYKVMDVEAESMTIKVTLNASHSMNYHSHKNRDEVWVVLAGTGKTIVDGYEQVV
                     KAGDVITMSAGCRHTIMADTELKLIEVQLGKDINVKDKQKFELEY"
     gene            complement(21176..22120)
                     /locus_tag="IWM35_001684"
     CDS             complement(21176..22120)
                     /locus_tag="IWM35_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019150212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HAP9919534.1"
                     /translation="MMEKDAKIYVAGHRGMAGSAIVRELNRQDYNNIITRTHKELDLC
                     RQDAVEAFFAQEKPDYVFLAAAKVGGIIANQNALADFMYENMILEMNVINSAWENGCK
                     KLQFLGSSCIYPRMAPQPMPESCLLTSELEKTNEAYALAKISGLKYCEFLNKQYGTDY
                     ISVMPTNLYGPNDNYHPTHSHVLPALIRRFHEAKEAGLPTVTCWGDGSPLREFLYVDD
                     LANLCVFLMNNYSGDETVNAGTGKELSIKELTEMVAKVIGYEGEILWDTSKPNGTPRK
                     LLDVSKATKLGWTYKTELEDGIRLSYEDFLNNPMRAER"
     gene            complement(22157..23170)
                     /locus_tag="IWM35_001685"
     CDS             complement(22157..23170)
                     /locus_tag="IWM35_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9919535.1"
                     /translation="MATLCRCEAKITLVCGANHLDFAKRNLEQELPKELIDRVRYRPI
                     NTDIGLINREGTLLVDTGKLTEICEYFLAKVPSLAKTEAEWLKREAVSAVLCDMPIWA
                     MDACDMAGVPFLYVGNFTWTELYREFLPEHIWKEYAKRYGKIQHTMLYALNNEEMLEF
                     LQNSEKTPTSVVARPIHNDEVQRIKSQHEKPPVFVALGMSARFKQPIDVSHAPYDFIT
                     TLGVPLVGENVTVISNSTPNTQDYIAAADYVITKAGWGTVAECILAQRPMALFARDTV
                     LEDRTTICKLQKLGLAISVTPENLHRVERVIEDLKQLDTSNFDTFYDAASEIADKLIS
                     LMQ"
     gene            complement(23250..24296)
                     /gene="gmd"
                     /locus_tag="IWM35_001686"
     CDS             complement(23250..24296)
                     /gene="gmd"
                     /locus_tag="IWM35_001686"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HAP9919536.1"
                     /translation="MKKALITGITGQDGSYLADFLLEKGYEVHGITRRASISNTARID
                     HLMGKITLHDGDLSDSSSLIRIISIVQPDEIYNLAAQSHVQVSFDVPEYSGDVDALGV
                     MRILEACRILGLTKKTKIYQASTSELYGKVEEVPQRETTPFHPYSPYAVAKQYGFWIT
                     KEYREAYGMFAVNGILFNHESERRGENFVTRKITLAAGRIAEGLQDHLELGNMDSLRD
                     WGYAKDYVECMWMILQHETPEDFVIATGEQHTVRDFTEKAFAANGMTIRWEGTGIDEK
                     GYDVETGKMLVCVNPQWFRPTDVDNLWGDPTKAKTVLGWNPQKTSYEELVNIMAKHDR
                     ELAKREAAMKNAVK"
BASE COUNT         7006 a         5591 c         4704 g         7365 t
ORIGIN      
        1 tttttaaacg gtgttccaga agtagcttct tcggaaataa gccgaaattt cacaaaaatt
       61 tgagaaataa tttttgtcaa tctcttctta tttcttgaag ctgaccactt ctgtcacaac
      121 ctcttcctta aacggtgttc aaaagcttgc atctgcggta ataagcccac caaacaaaaa
      181 atatgaagag ctatttttgc ttgctgttct tattacttga tgcagaacag ctttttcaca
      241 acctctcata cacggtgttt aatcagctga ccttcggtat tagctcaaaa aacggaaaaa
      301 tatgtgaaac tatttttccg tttgtttctc taatactcag gtcaaaacac tgatttcaca
      361 acctctttta ataacgaaga atcgctgtca gactcttttc atctggcgca tctttttgat
      421 ccaaaataaa cactttcccg ccattaatga tcacatcatc tgctaatgca ttcaacactt
      481 gacgccgatc atattctgtt tcaggatctt caccgaaacc atcagctaag tttgacgtag
      541 cgataaataa atgtgacact tttccttctt tcgcagctgg aacgatatca actaactgat
      601 cgatgaattt cttatctaag agtttattgt attcttttga ttcttgagct gcgagttcac
      661 ttgacagctt cttcgcccct tcttcgatat ctctaaagga tagctgcgca ggagaagcag
      721 aaatcgcaac gtctgcacaa taatagggat ttttagcaat ttttttgaac atcgtctgat
      781 tttctggtaa tgcaaataaa taaatcggcc attgttcagg attttcgaaa gaatccttca
      841 agtacgtatc aacagcttgg taatagttga cccaatcgat ctctacttct tcatcttttg
      901 gatttacgcc atggaaagtt actccatctc gggctccgcc tcctccatta gaagagaagt
      961 tcaaaccgcc acctgtcagt tcagatccca atgcagtaac gacatcaacc ggtgcccctt
     1021 ctggtagaga tacttctttt acttgtttgt tgctgacctc atacaatttc atggagtcgc
     1081 ggttcaatcc caataaataa taagaatagt tgaactgccc atttttgata atagccagta
     1141 aataaggaag atcacttaca tagtattgat cgtctaccgg gatactcaaa cgatggatat
     1201 aggtctggtc agcagttata atgaccgcta cactcgtagt tgcactacgc caaaacgaag
     1261 gatctcctaa caaattttca attttttctt ggaacgggga ccagtcatgt tcggaatatt
     1321 ttttctcaaa acgtttcttt gcttctttcg caaaattttt gaatttgatc tgatcttttt
     1381 ccacatcttg atgggcgaca tgcgtattca gcatgatcgt gacgaatggt cctttagcgc
     1441 cttctgaagt caattgtgtc aataattctt tatccttttt tgccataaac gtacccctcc
     1501 taaaaaaatc attcctttgt ttctatctaa agtctaccac aacactattt ttccatacaa
     1561 taaaacgtat ctaaaaaaat aaaacggttt ctacggtttt aaatttagct atttttttaa
     1621 gttatcttca taatttcctc aattatttag ccccagtctt caactattct tcatgaaatg
     1681 ctgataaact aagaaaaaga ataaaagagg tggatcttat gcatttgatt atgattttca
     1741 gtttattaag tattatcagt ttatttgctt tcagcgcttc ccgcttatta gatgcggaaa
     1801 cacagcctat tcctgtaaaa gtacgtaaaa acgaccgcta aacttccata acgcgcgttc
     1861 ttatcctaaa gttttttcag aaacattgaa actctctccc ctctctgata aaattttaat
     1921 ttgtagcgaa acaaattaat tacaaggaga tcggatatgt ccataaaaaa aacacgtcta
     1981 gtcctattat tggctagtct cattctttgc cttgcttctt ttctaccagc tgttgctgtg
     2041 caagctgaag atacgttcaa agtaaatgca aaagcagctt ttgctgtaga tgcgcaaagc
     2101 ggtaagattc tttatgatca agatggagaa aaaaccatgg ggatcgcttc aatcacaaaa
     2161 atcatcggtc tttacatcgt ccttgatcaa gtaaaagaag gaaaactgtc atggggtgat
     2221 aaagtttcga tttcagatta tgcagaaaat ttgagtatca ccccagattt atcgaatgtt
     2281 cctttgcata aagaaaacac ctacactgtc aaagaattat tcgactctgc aatcatccag
     2341 tcagccaatg cttcaatggt ggccttggca gaaaaaatct ctggcagcga agctaaattc
     2401 actgaacgaa tgaaagaaca attgaaagat tggggaatca aagatgcaac aatcgttaat
     2461 gcatcaggat tgaacaactc ttatcttggc gaaaaccgac cagaaggcac tggagaaaac
     2521 gacgaaaatc aaatgtctgc tcaagatgtg gccatcgttg cccgccattt gatcttggat
     2581 ttcccagaaa tactggatgt atcaagtact acgacccaaa tgtttggcga aaatacgcaa
     2641 tcaccagtgg aaatggtcaa ctggaactgg atgctcccag gattcgttaa ttacaaggaa
     2701 ggcgtggacg gattaaaaac tggtacgact gagcttgcag gtgcttgttt cgttggtacg
     2761 atcactaaaa acgatcaacg gatcatcact gtcgtattga atgcggacgg ccatgcggaa
     2821 aacccgagtg cccgcttcaa tgaaacagac cgtttgatgg actactgcta tgacaattgg
     2881 tcagaaaaag agctcggaaa agctaacgca agtattccta gtctaaaaac cattgatgta
     2941 aaagacggaa aagaatcaac agttcctgtt gtgcttaaaa gccctgtaaa agtatgggta
     3001 cgaaacgata tggatactgg caaattaacg atcacgccaa cacttgacaa gaagcaagtc
     3061 actgacaatg agatccaagc acctatggat aaaggtacaa aaatcggtac cgctacgatc
     3121 accttggcag acgataaatt aggctattta gaagacaaca atgcaccatc aacagagatc
     3181 attacggata aaacagtgga aaaagcgaat atattcgtga ttggctggcg acgcgtcgca
     3241 gaatttttcg gcaacttgtt ttaattgaac aataagaaaa acatctttcc atcggaaaga
     3301 tgtttttttg ctttttctag tttaaataac agcttttttc atctattttc gttatactgt
     3361 ataagaaaaa ggagttgaat ttatgaatct aaccagagat ctgatctttt tagttctcta
     3421 tcttattggg gtattctcta tcgtttattg gcgaaaacag aagaaggatc cttccttgtt
     3481 cttttacggc agtacgtgga catttacggt aatcttaact tattttgtcg tgttggaaat
     3541 tttttattcg accttttatt ttttgattcc attgttgttc tttagtatct ttgctttttc
     3601 ttatttcaca gaaaaacgaa ggctactgaa tggtctttta ttcaatgttt tactcatcag
     3661 tttcggcatc tacttgtttg tcttgctgta tgaaacgcaa aatatttttc ttggcggact
     3721 gatcgccttg atcacgattc cattgctact tgtcctttta tttggtattt atggattgat
     3781 tgtttttctt ttttggaacg gggtcacagt tttacgtaga gaatcgcatt cattagctaa
     3841 tcttttaacg ttgatactag ccatttttct tacactgttt cttgtttttg actttttctt
     3901 gttaaaatat ttgccgcaat ggataaatgc tttgtttttc tgcgttcccc tgatcttgat
     3961 ctatctattt atcgtattct acaacttttt gactgtctca ttcttgtatc agttcaaccg
     4021 gcctcgatac aatcaagact tcatcgttgt gttaggcgct ggattgatca atggagaaac
     4081 agtttctcct cttctagcca agcgaatcaa taaggcaatt gctttttatc gggctcaaag
     4141 ccgtgctaca ttaaatccgc ccatcttatt gatgtctggc gggcaaggag cagacgaaaa
     4201 agtaccagag gccattgcga tgaaacaata tgccatggaa caaggaatcc ctgaaagaga
     4261 cattcttgtc gaaaccaatt cgacaaccac tttggaaaac atgctctatt caaaggaaat
     4321 catggatcag caaatgaaag ggccataccg cgcgatcttt agttccaaca attaccacat
     4381 ttttagagcc ggtctttatg cgcggcaagc caaattgaaa gcaaatggta ttggtgcaaa
     4441 aacagctttc tactacttgc ctaatgcgtt tttaagagaa tacctcgcca tcttgatgat
     4501 gaacaaaaaa agacatatga tcatttgcgg attgcttttc gcaggctccg cattcttatc
     4561 gctgattact ttacttttct aaaaaaacaa ggtgtgacaa aacatatgtc acaccttgtt
     4621 tttttctttc attctcttaa ctattggcaa aaagagtatc ccttttttga ccgctacttg
     4681 ctgagcagaa taaataccgc tgttgcctga acacataaag ctgatcaagc agatcataaa
     4741 gaagtatact gccgcttgtg aaccgaacaa ttcgatcccc atcacaaagc aggcaatcgg
     4801 tgtatttgtc gcaccagaaa atactccgat aaagcccaat cctgcaagaa aaggaacaga
     4861 aacatggagc aaaggcgcta gtgctgcacc aagcgttgca ccaatttcaa acaatggtgt
     4921 cacttctccc ccttgatatc ccgcgcctaa agacaaaact gtgaagaata acttcccaat
     4981 aaaatcatag acatgtgcct gtccattaaa tgattcttga agtaacggaa gactcaatcc
     5041 gagataacgt tgtgtttgca aaatcaatgc tgctgccaca actacagctc cacctatcgt
     5101 atttcgacaa acaggatgag gaatccattg tgcatatcgc gcttttaaaa agacgatcga
     5161 acggctgaat acccaaccaa tcaaaccaaa agcaatccca gcaacaaaaa ctttgaagaa
     5221 cagcaacata ctccattcag gaatcacacc catttggtaa tgtagatgag aaacaccata
     5281 gctttctgtc atgaaatttg caaacagtgc agcaaagaag ctagggaaga tcgcttctgt
     5341 tcggactttc ccgattgcca acacttctaa tccaaataat gttccagcta aaggtgtgcc
     5401 aaatacagaa ctaaaacccg cactgattcc actgataatc aagatttccc gttctgattt
     5461 atccaatcgg aacaaacgtg ccgtattatc tgctaacgct ccccccattt ggactgctgt
     5521 tccttcacgg ccaacagatc ccccaaataa atgggtagtt atggttccaa acagtgtaag
     5581 tggaataagc cgcaacggga ttttttcttc tccaccattt ccttgatcga tcactaagtt
     5641 gtttccacga ctagaaagtc cgccgtgata cgcatagaga taagcaaaaa gcgcaccact
     5701 aactggtagt agacaaagga gccaaggatg tgatagtcgg atatctgtca caactgtcag
     5761 gctatttaag aaaaaagcag acaagctccc catgaatata ccgagaatcg ctgtgatcac
     5821 cagccacttc agcatataca ctaaaatgcg tactggactc ggtaattgtt tttccataaa
     5881 aaattcctcc tcaataaaaa aacgcctact aaagaaaggg gctgtcccca gactttagta
     5941 ggcgtcatta gctatctttc gttagcattt aaaggcgaac accatcacct attcacttcc
     6001 attaaaagat agcatatttt tctgtaaaaa acaataaaat tatcgttctt tctttacacg
     6061 ttcagcaaat tcatagaaag gagccaattt cgttaactgt tgattacatt ggtcactatc
     6121 atgacaaata taattcccct ttttcgtata cgtgccatca gaacctgctt tcgttgtaga
     6181 aagaaaaagc gaaacattcg atgttttctg acagattgca cagattccct tatttatcgt
     6241 tggtgataaa gtaccgctaa tcccttttaa tttttcatct tcgtaataga ccatgaattt
     6301 tttctgcgta cctggatcat tccagccgat gtacgtatgt tcttgcaagt cgatttcatt
     6361 ccaagctggt tgtttgagtt ttttcacttt tcggaataat ttctcgattt gtggtttgct
     6421 aggctgcata aacggctgaa cgatttgctt cagctcctcc aatgctcttt ctgcttgcgc
     6481 ttttgtcagt tttacgtcta aaactgtcgt taaaaatgta tcgattctgg cagttcgtga
     6541 tctaatatgt ctcgaatctt ttcagctgct agtgcctgaa ctgtttgtac cgtttttgga
     6601 tcattgacag atgagtacgc atttaataac tgttctactt ctcttattat aaaaaaatat
     6661 tggtatggtt ttattgtttg ttccatgatt ttaaagtcct ttcttctaac caaagtgcat
     6721 actttgtaga aaaaagtgat cttctgcaaa gtatgtgtta cctgaagatc ctggactctt
     6781 ttaaataaga tacgttttgc atcttatcat cacccccttt aatgcttatt attctatgta
     6841 catgattcat tcgataaata aaagtaaacc gtatcttgaa cagatagtca actgcaaaca
     6901 gaaaaaagga ctgtgacaaa agtcgtgtca aagtcccttg tttcgaatga atggtgttca
     6961 aaagcttgca tctgcggtaa taagccgaaa cttcacaaaa atttgagaag taattttcgt
     7021 aaattccttc ttatttcttg aagctgacca cttctgtcac aacctcaata tggtctatta
     7081 ttggttttac ctgttaaatc gaggattctt tcccaaagta acgttgccat ctcttacgca
     7141 tacttgcaga catgggctga gccgcttctt tgactgcgca gtatttgtcc acgaatgtca
     7201 caatatatcc ttctttatac ttaggcggtg cgatcgttgc accccacatg tgcttcaaaa
     7261 tgatatctcg ctcaagatcc gacaaatcgg tgattttttc tgcattcttt acagcaattc
     7321 ttggatggat atacgcatgt gtcccctcgt caaatttcgt cgtacgccaa tcatagtaga
     7381 acagatcatg caaaagtcca gcacgcgctg ttgctttagc attgccaccc catttttttg
     7441 cgagcagata actatagtaa gaaacactga tggaatgctc caaacgagtg gaatgaacat
     7501 gctgtgtgta attcgctaat tttttcactg cttctgtttc taaaagatct tgtacataag
     7561 acatatattc ttcgtcttca cgccattttt ctgtcataat tatcgatttg ccaccttttt
     7621 gaatttcatt ttcttctgaa attgatctaa gtataccacc agtgttcgga ttattctagt
     7681 cgctctagat aaaatagcta aagcttaata gttccttaat cataaagaaa aatgataaag
     7741 aacgatccct gctgcgactc cgacattcaa tgattccgca tttcctttca ttgggatata
     7801 tacgttctga tcagtcgaag acaagatttc ctgacttact ccctgacctt cgtttcccat
     7861 gattatagcg aaatcctctg ttttttctac tttgtctaat gcatgtgctt cttcatttaa
     7921 ttctgtaccg tacaccgtaa accctttgcc tttcattaca gggatcactt ctgccaaatc
     7981 tttttgtaac acaggcaaat gataattgct tccctgcatg cttcgcagca cctttgtact
     8041 ataaatatct gccgttcctt tacctaataa aacaccagcc ataccaaacg catctgccgt
     8101 acggatcatc gttcccacat ttccaggatc ctgtacatta tctagtaaaa gccagcctcc
     8161 tgaaaaatct ggcgtatctt cagatgttaa cgtcatcact gctaagatcc cttgcggtgt
     8221 aggtagatct gacatagcgg tcatgacctc atctgaaact agaaaagtag aaatctcttt
     8281 gtttacagcc agccagtctc cccattcttt catacctctc tgtgtcacaa aaagttcttt
     8341 tatttctgca tcagacgacg cagcttcttc gatcagatgg aacccttcca aaagatattc
     8401 tgccttttct tctctgtatt tctttttatg tagttttttt aattctttga ttcttggatt
     8461 ttttactgat tggatctctt tcacattcct cacctcaact gattatatca tggaataggt
     8521 gcttgagctt gtttcatcct aaaaaaatcg gtatcattga tttataaaac aggaaagaaa
     8581 ggtgacgaac atgcgcaaag ttaaaatgaa tgtccaagga cgtgtccagg gcgtgggatt
     8641 tcgctatatg accaagatgg ttgctgatca gttaggcgtg accggaacgg taaaaaacga
     8701 agacgacggc tcagtcacaa tcgaagcaat cggaaatgac gacatcatcc aaaaatttat
     8761 cgaagaagtc aagaagtctc cgagcccaag tggacgagtg cagtatgtgg atgtccaaga
     8821 agatccgatg attgaggaac gaaagaaatt tgatgtggtg ggataaggtt gtttttgaag
     8881 cgctttgcct cgagtattaa ggagaaacat tttccaaata gcttctcata ttttgaaatg
     8941 taatgactta ataccgagag gcagctgata aaacaccgtc aataaggttg tgaaaaagct
     9001 ggtctgcatc aagcattaag gactggttgt gaaaaatagc tctccatatt tttcaacagg
     9061 ctggacttaa tgccgtagat gcaagctttt gaacatcgtc gataaggttg cgaaaaagct
     9121 gatctgcatc aagcattaag gactggttgt gaaaaagtcg cttagttttg agtattaagg
     9181 aaaattttgt gaaaatggct ttccacattt ttgcataaat ttgacttaat accaagaaac
     9241 tgacttttga acatcgtcga taaggttgtt tttgaggcac tttgcctcaa gtattaaaga
     9301 caggtagtaa aaaagctgat ctataacttg caataaattt gcttaaccca tattttccag
     9361 acagaacaaa aaagttttta gttttttaaa taagaaagaa acacttggaa tcactctttt
     9421 ctttctaagt atttctttct tgattttttg tcttttttca aaatttttcg ctacgaaaaa
     9481 gaacgaatat ttatatgaaa tttctatgaa gttgattgaa ttccatgcgc ttttttagta
     9541 tagttaagtt tgtgtaaaaa ttatagaaga ggaagtattc tatgaataaa tggaaaaact
     9601 ggttattggg atccggactt gtcagtatcc tgctcttttt atctggctgt gtgcggatgg
     9661 attcaaacgg aaaccctgac acttcaggaa tcgtttatcg agtactcgtc catcctatgg
     9721 gccaagcgat tacatatcta gttgagaatt tcaattggtc ttacggctgg gcagttattg
     9781 caatgactgt gatcgttcgt atcatcatct tgccactagg tatcagtcaa tcgaaaaaaa
     9841 caatgatcca atctgaaaaa atgcaagcgt taaaaccaca agttgaagca gcacaacaaa
     9901 agctaaaagc tgcaacaaca cgtgaagaac aaatggcagc acaagcagaa atgcagcaag
     9961 tatatcgcga aaatggattg agtatgaccg gcggaatcgg ctgtctgcct ctattgatcc
    10021 aaatgccgat cttctctgcc ttgtatttta cggctcgcta cacagaaggt atccgtgaat
    10081 catcttttta cggcatcgat ctaggctcac caagtatggt gcttgtggcc atcgcagggg
    10141 gcgcttatct gcttcaagga tatatttcaa caatcggtat tcctgaagaa cagaaaaaga
    10201 cgatgcgtac gatgctgatc gtctctcctg ctatgatcgt cttcatgtcg atcagtgcgc
    10261 cagccggtgt tacactttac tgggtagtcg gtggtgtgtt cagctgtctg caaacattca
    10321 tcacaaacgt tataatgaaa ccgcgtttga aagcacaagt ggcagaagag ttgaaacaaa
    10381 acccgccaaa acaagtcgtt actccaagaa aagacgtgac agaatcagca gcaaagccag
    10441 ctgacaacaa acaagcaaca aaacaaatca aacaaccaaa aaacagtaca aacaacaaag
    10501 gccgcaacgc aggaaggcag cagaagcgga aataaaaata tatgacaaaa aactgtagac
    10561 aaataaaaaa atttctttgt tctacagttt ttttgttaca attaaagaaa acgttcgcat
    10621 ttcgaggagg aatgtataat gatgtccaaa taatcaatgt atagtcgaat ggaagaagac
    10681 aaaatttcgc tagatgcact tgttcctaaa aatcatttag tccgtaaaat tgacaaatta
    10741 ttgtcatttg attttattta tctcattgtt gaatcaactt actcgaccat tggtcgccct
    10801 agcgttgatc ctgtcgtctt agtaaaatta gtattcattc aatatttatt tggaatttgt
    10861 tcgatgcgtc agaccattaa ataagttgaa acaaacctag cttatcgttg gtttcttggg
    10921 cttagtttaa ttgataaagt tcctcatttt tcaacttttg gtaaaaacta tgcacggcgt
    10981 ttcagagaaa ccgatttgtt tgaacagata tttatcatta ttctcgaata agcagttgat
    11041 gctagattta ttcatgaaga aattttgtat atggattcaa ctcatattaa agcgaatgcg
    11101 aacaaacgta tttttacaag agatatggca cataaagaag ccattttata ccaaaaacaa
    11161 ttagaagaag aaatcaatga ggcaagaatt gccgaaggca aacgtccttt tggttcaatc
    11221 attcgtcagg aggtggtcga aagaaaattt agcatcgcag atccagaaag tggttattat
    11281 gtaaaaacag aacgagaaaa acagtttgct tattctgctc ataccgttgg tgatgaaaat
    11341 ggttttgtat tagatgtcat gattacgcct ggaaatctac atgatagccg aatgctcgtg
    11401 ccacaaatcg aacgcgttaa atcacgttat ggtgtagctg ctaatgctgc gtacaaaaca
    11461 ccttggaacg tcaagtattt aatcgaacga aaactacgac ccattctccc ttatatcagt
    11521 ttcgcacaac tactcgagat ggctataaaa aatatgtatc taaatctttt atttgtgaaa
    11581 gttgtccatt attgaaaaat tgtacagaaa gccaaaaaca ccaaaaagaa atacatcgac
    11641 atatttggga agactactta gatgaagcag aacatttacg ccatacggtg tacaatcgtg
    11701 aaaaatataa aaaaagaaaa gaaacgattg aacgcatttt tgcagacgca aaggaaaaac
    11761 atggaatgcg ctacaccaag tatcgaggct tggaaaaaat caggtgcata caatgcttgt
    11821 ttttgtcgca atgaatctga aaaaattagc tacttggcag tgggagcata tgtcgttata
    11881 tcttttatta tacaaaaata gacaaaagta tcagtctaca ctcttagtaa agaggaaact
    11941 gatgcttttg tctacagtct tagagagtgg gacaatagtt ttttcttctt tacgtctttt
    12001 cctctccatg tattttccta attttcttaa tttaacgcca tgagtgccaa gccaatgtca
    12061 atcttggctc cttgtttcct tcataagtgg aaatccgttt ccgatagatc gaaccttctt
    12121 tttcctctaa aagttttttt acattctgct ttgaaataac gccatgtatt attcactaaa
    12181 atttgtcggt ttcgctcact ttttgctttc gtacattgac tacgaacagg tcagcaccga
    12241 ccactttcac attcgtaaat tttgaagaca cgtataaatc cacttttatc ttttcttcga
    12301 ctaaagcgtt gaccgcaaaa gcgacatctg tttcagaaat atattgttcc atatctaatg
    12361 gtaaaatttt ttggttgatg gtataatttt taaacataaa gcactcgctt cctttctgat
    12421 tgggtttggt cactttaatc ctacagggat gagtgctttt tgtgtacctt aaaatcagta
    12481 aatatccatc cgtgaaaacg ggtggctttt taacagccct agaagggctc accactcaca
    12541 gccctatctc tctttaacta tttaaatgga tctacatact cttgtttact tatactgtct
    12601 ctaattcgat catctgcttc ttgttctcaa atatatattt caattgtttt ttgatttaac
    12661 ccaaccgtac tcacgtagta tcctttggtc caaaagcttt tattcccata cttatatttc
    12721 aaatttgcat gtttttcatg gattaaggtt gcacttcgtc cttttaagta gcccataaaa
    12781 ctagaaatac taatctttgg cggtattctt actaacatat gaatgtgatc aggcattgca
    12841 tgagcttcaa tgatttcaac atctttcctc tcacacaatt ttcttaatat ctggccaata
    12901 cttactctta actttccata aatcactttt cgtcgatact tcggggtaaa gactatgtga
    12961 tacttacaat cccatctcgt atgtgctaaa cttttatcgc taaagctatt tcgtccacgc
    13021 gtagaaacgc gcgttttttt gtttcgtacg ctacgccaac gaaactgact aaagtcaata
    13081 aaaaaagtgg ggcaataatc agattttttt ctgactattg tcccactctc tttttattac
    13141 tcttcatctt ctactattcc actaattaat tagttgacag atacaatgct ttcacatcct
    13201 gcacgaacaa actcgggctc tgaaccaatc acttgcgagt tgataaattc aatgaccata
    13261 ggaagattaa tccgacataa agtttaatct gttgtccttc cattatcagt ttagaaatga
    13321 tattagtagg tgtaccgcct aaaagatcgc atagtaccac aaaatcattt aacgaatcta
    13381 tgacagtaag agcttttgtc tgtatgtttc agtgccatct tctggtaata aaggtaacgt
    13441 gtagatattc tcttgaggtc ccataatcat ttttgtactt tttttcaatt cctcacaaaa
    13501 ttttccgtga ctaatcaata tcaattttct actcattttt ggctctccct tatttcgaaa
    13561 tcacacacaa tgcagataaa gccacacaca agtaatacga taaaaatcgc tcgagttggg
    13621 tcatattttt ctttcctaac aaccaataaa cacctcgacg atcccagcga gtaagatttt
    13681 agataatatc atatcgatat agttttgcat attcaatgtc acatcgccaa tcgttggtac
    13741 cataaaaatt taatatcaat cattgttgca actaatgctc ctaccatgaa cactcttaat
    13801 aaagtcgcta cgtccgttaa tacagttaag cgagattaca ctagatgatc tgttcctgcc
    13861 aaggcaagag ccagaagcgg ttgcctgccc atttgccttt ggtgtgtcgg agctgtccga
    13921 taaagttcac cgcccgatcc gctctggctt tgtcgtagtg acattccgaa agcatgaatc
    13981 ggcttggtgt gtattccttt aatttgggat agccctttgg tctggtttct gccatcagct
    14041 accacctccc aacagagcct ccatcgcatc ttctgctccg acaccaccgc catcaccggc
    14101 aatgatacgg cttctggacg aaggcgtcag accgaactgc tcggaaaatc ggttcataat
    14161 tttcagatag gtctgggcaa taaacacctg tggtacctgc tgccaatagc cggatagggt
    14221 tttcacgatg ataccgtgct gggtaatgaa ttcctcggct tccttccagc gggcgtaggc
    14281 ttggcactaa ccggcaaagg cagccatatc cacatctgtc aagattccga tggcctccat
    14341 ctgctttgcc agacgcttcc attccttctt tgcctctggt tccagccact tcggacaggt
    14401 cggtgccttc ttttccggcg ttgactcttt tgcattgagt gggcgattgc ccggatttcc
    14461 ctccagcttc ttcatggctg tcggggttga ttttcttcct ctggttgcca cagtcgtcac
    14521 ctcccttcaa tgtatttctg ccagtagaca gagaacagag ccaagtcgaa acctgactct
    14581 cttctctatc tgctaacttg tatttcagtc cactcttctg atgaatcgca cccgcaggat
    14641 tttcaagatg ccgcaggcaa attccaagaa gtttctgacc gcatcataac catcgtagcc
    14701 gtgtagccac atccttccac ctcgccgccg tcctcgtcgc tgatataaat gattctgtat
    14761 tcgctcatct gttgttttct ccagtctttt gtttttttgt agtacatata tcactctaaa
    14821 agtgattatt atcaagtcgt ttgaaagcca taatgtacac aatcattcga ctggtttttg
    14881 gttcataatt gaccgccaaa gtgagcaaaa gaaagggacc tctgtctgtt tcagaagtcc
    14941 ctttcggtat ttggttctta gtggttcagg ctgtattctc aaacctcata ctcatcttcg
    15001 aggtctgcct ctgcaatgat ctggccgatg ctgtcgtaac gctggacctg catctggatg
    15061 gcatccttgt ctgctccctc gtccatcatc ttcagcagct tgtctgattc ctttttgtag
    15121 gcccagaaaa ggatctcctg aaggctggtc tcctgctttt tcttgcgaag ggcgaccttc
    15181 ttctcttcca ccgcctcgtt caacttggtg aactctgccc aagcaaggat accgtctcgc
    15241 ttgattgcga tctccacatc cttcagctcc gggatggtct ttgcaccctt gatctccttc
    15301 aaatatttct ttgccttttc gctcattgcc attagcgttt cctccttgtt tttgggattt
    15361 tcccctttgg ttgtgtgtat attgccatag tctctcgact atatcaagtc aatccacagg
    15421 catatactac acaatcattt ggagctgtat ttgtatacat tatgccgatt tatgagagaa
    15481 gaaaaggctc cagccgttcg actggaacct tgggggggtc ggtttcttag gcggcgtggc
    15541 ggtgtagacc ttgaggtagc tgatgttctt ccagacgtcc gtgcccttca gtctgaccat
    15601 gccgtccagc tctcggtcga gggtgaactt gcggaaaagc ttgatgcggt tgaggctgtt
    15661 tgctagctcc ttgccgttct tggcatccag aactgcgtcc tcgatctccg caatactaat
    15721 ttcctgtcta aaccaaaggt tgctcaaggc ttcgggaatg ccgtttgcgg tctgcagggg
    15781 gttgaaagtt tccaatacag caggggtcga aaccacctgt ccgagctgga attttgcgtt
    15841 ccctaaaatc atctgcataa tttcttccat gtgaatcatc catttctgcc gttgtggtat
    15901 tccctttggc agtacagtac tatatatcac tctgaatcgt acatttatca agaagcggat
    15961 ttttgtggaa tcaccaattc gatataatag taattgcatg aaaaatctaa gcactggatt
    16021 ggtaggtgat gattatacgt gacgatcaca tttacacctg tgattcctgc cactacagct
    16081 tccctgccga cgaacagcca gagcgttgtc ctgactgcga aaagacagca actcggctgg
    16141 ataccgaaat cgaaactgaa gattactatc gtgttcgtgc tgaaatcaaa gctgagatta
    16201 aagctttgaa tgcgggttaa cgacaggtac tgaccggcgg ctcaacaccg ccggcttttt
    16261 attaccacat cttgcggaat tcgctgggac gaacaccttc caccttgaaa aaagttctgg
    16321 aaaaatagtt tgcatctgtt atgccaattt ttgttgcaat ctcttccata cttagatttg
    16381 taaagcgtag caagtgcttg gcctgtgtga ttcgtttctg attcagataa ccgacaacag
    16441 ttgtaccgta agtttcacga aaaatcttag ctaagtactg tttattgata aaaaactctg
    16501 cactcaactg atccagcgta atcttctccg tgtaatggtc atccagatat tgttttatgt
    16561 tctgcaattc catttttttt gtagagaaat tcggcacttt gtcaggattc caagaatctt
    16621 ccatcaaatg catcagcagt tcactgagta gaacattgat tttcatatcc cggacatact
    16681 cctgtccctc tgccaccata tgaaggctcc tcagcaagtc agcatactta tctgtttttg
    16741 atgttttaaa gaccggttga ccacctcgtt caaggtattt tttgtagact tccgtcatac
    16801 caatgccatt aaaatggcac caccgcaaag tccacaaatc atcttctgat gactcatgga
    16861 catacggctt caaacagtca ataaacacca tatctcctac ttgaagcgta tatttctgcc
    16921 ccttatactc caatgtgcct gaaccaactt ctaccaggaa acaaaggtaa gatgacaatc
    16981 ctgtacgcct agatgtatgt gccttcagag cagtcagctg tccaatttcc tgcaaataca
    17041 aaagagagga tcttgcaaag aacgatgccg tgtacagcac acgatttgac ttcaccgatt
    17101 tcaaagcgtt agaaaacagt tcctccatta caagatttct cctttctttt cttactcgaa
    17161 catttccgcc agttcagcag ccataatagc ttccatagct tcggcggctt ccttgttctc
    17221 tgcgctgacg ctgatatagg tcttcagctt cggctcagta ccagaaggac gaaccacaac
    17281 agagcagtta tcttccagca taaacttcaa aacatcagac ttcggcagac cattcaggcc
    17341 cttactgtag tctaaagttt caataacctt cttaccacca attatatcga gacccttgtg
    17401 gaattcaacc ataatttcct gcatcttggt aaaacctgcc gcaccgtcaa actcgaagga
    17461 atgcagggtg ttcaaacaat agccatactt tgtataaatc tcgttcagct tatccagcag
    17521 gctgataccc tgagtcttgt agtaagagaa catttcacag attagataag cagcgttaac
    17581 accgtccttg tcacggacat agctaccgga cagatagccg taagattcct caaagccgaa
    17641 gatatagctg tcaaccttac cagccttttc caacatacca atctgctcac cgataaactt
    17701 aaagccggtc agcacatcga tggtacgaac accatagttg gatgcgatac gttcacccaa
    17761 atccatggtg acgatggtct tgaccatcac agggtcagca ggcatttttc catgcttaat
    17821 tctctgagaa cagatgtagt ccagaagtag cagaccagtc tcattaccag acaacagaac
    17881 atagtcacca tgtgcgctct taactgcgat accaacacgg tcgcagtcag ggtcagtggc
    17941 caacagcaga tccgcattgt acttcttggc atactccata cccagagaca tagcttcctt
    18001 aatttcaggg ttcggataag gacaagttgg gaaattaccg tccggcagac gctgttcttc
    18061 cacaacggtc acattgctgt atcccatctc tgccagggtg cgggtaacag gcaccagacc
    18121 ggtaccattc agaggagaat acacgatagc aacattcttg tccacttctt cgccaaacag
    18181 gacggactgc cccttcactt cttcgatgaa agcagtcaga acttccgact gaatggatgc
    18241 aatcttagaa gcatcgccat ccatctttac atcagcaaag atgtccagct tttcgatttc
    18301 tgccagaatt gcagtagcag cctcggtagt aatctgacag ccgtctgcac cgtatacctt
    18361 gtagccgtta tatttagaag ggttatgaga tgccgtaatc ataacaccgg catctgcctt
    18421 cagataacga gtcgcaaaag atactgtagg aacaggaagc agttctggcc acaagtgcac
    18481 ctgaatacca tttgcagcaa agatgtctgc ggcagtcttt gcaaacacat cagatttgat
    18541 acgggaatca tagccaacca ccacggaagc ggcttcatag ttcttattca gatagtctgc
    18601 cagaccttgg cttgccttac ctacggtgtg tacgttcatg cgattagtgc ctgcaccgat
    18661 ggtaccacgc agaccaccag taccgaaaga caaattttta tagaaagagt cctcaatgtc
    18721 cttctcagaa taggaacgca gttcttctat aatatctgca ttggcatgat tcagccaacg
    18781 ctcatattct actttataat tcatattatt atcctctcac atccagaaac tgaatcgacc
    18841 cgtgaatgct gatttcagta gaatcggccg gcacaaaaag gcagtccccc ttatagatgt
    18901 ccaacgattc attatcaaat cgaacggtac cacagccatt gacacacagc aacacacgga
    18961 agctgattga atccgcacga tagtgtaccg tctgtcttct ctcagtgttg acaatcatac
    19021 gagagacctc gaaatgcttg catctgcaaa gcaattccgc agctacacca gcacgatatt
    19081 ttagaacacg aagcggctgt ttcggttctg acaacccttt caagttcgct acttccagcg
    19141 ctttgtctac atgaagttct ctctttttac catttttgtc aacacggtca taatcgtaga
    19201 gacgataagt cagattggaa ttttcctgaa tttctgcaat tagtgcaccc gcaccaatcg
    19261 catgaatcgt tcccgcctca ataaagaaca catcattctt tttgaccgga accttctgaa
    19321 gataacgctc aacagttccc tgagcaatcg cttcacggat cacagcttct tctgttattt
    19381 tctgcagccc atagattaag ctggcatcca gtacatacca catctctgtc tttccaagct
    19441 gcccgttttc tttttcaatt gcatattcat ctgtgggatg tacctgaaca gacaaatcct
    19501 ttttcgcatc aatgaatttg atcaggatcg gcaattctcc tttggtctgc ggatgcgttc
    19561 ccagaaactc tgggtgttct tgcaacacct ccgtcaaggt catcccctga tactctgacc
    19621 agacacaaca aagctcggtc catccgggtg cgtagagcat tccaagtttc cgcaagagga
    19681 gacatctcaa ttccttttgc aaattcatca ttcaggcggt tcccccccac agataatcct
    19741 ttgcggaagg cttcaataaa aacggtttct gtataacgag ccctcctccg ttttttaata
    19801 ctctaactca aatttctgtt tatctttaac attgatatcc ttgccaagct gtacttcaat
    19861 cagcttcagc tcagtatctg ccataatggt atgccgacag ccagcactca tggtgatcac
    19921 atcgccagcc ttaacaacct gctcatatcc atccacgatg gtcttaccag taccggccag
    19981 aaccacccac acctcgtcac ggttcttgtg gctgtgataa ttcatggagt gactagcatt
    20041 cagagtaacc ttaatggtca tgctctctgc ttctacatcc ataaccttat aactacccca
    20101 agacttctct gcgaacatga tctgctgctc gaacttgtca acgaaaggct taatatagct
    20161 ggactgttcc ttatcggaaa ccagaatacc ttcccgagat gcacagatga ccacatcatg
    20221 cagacccata gccaacacag gaacatccag ttcgttcagg atgtgtacgc catcgcaatt
    20281 ctcattcata acacctttac cgataatgga ttcctccata ccttcggtca gagtattcca
    20341 agtccccaga tccttccaca taccagcaaa acgctgtacc tgaatcttgt cctcatgctc
    20401 aacaacggca tagtcgaagg aaatcttagt cagagtatcg tatttgttga acagatcctg
    20461 ataatctaca aagtcaatca gctgatgtgc acgctccatg acatacttca gcttgtagac
    20521 aaacacacca ccgttccaca gtgcaccctg tgcgatatat tcctttgcca cagcctcaga
    20581 aggtttttcc ttgaaggtat ttacgaaagc agtctgctct gcgctctcag gaatgatgta
    20641 gccgtacttc tcggaaggat aagtcggttc aatacccatg agcaccagat tagcttcgcc
    20701 tttgtcagcc tgatcggaca gagatttcag ggcctcaaaa tagtcatctt ctacgtaagg
    20761 gtcaaccggg caaacaacta cggactcttc tggatcaatg cccatcacat ctaccagata
    20821 agcggtagcc aatgcgattg ccgggaaggt atcgcggcga caaggctcta cagatacacc
    20881 cacatcctca ccaagttggt tatggatagc agaaacctgt gtcttagagg ttgcaatcgt
    20941 aacagttgcg tcgttatcta ccttcttaat ctggcggtag acacgctgaa ccatggattc
    21001 atatccatct tcagttttga aaatcttaat gaactgcttg gaacggatgt cattagacag
    21061 cggccaaagg cgcttaccag aaccgccaga cagtaaaaca atattcataa tgatctctcc
    21121 tttttataag aaagaggctg ctgcaatcct caaaaatcac agcaacctcg gtaaattagc
    21181 gttcagcacg catcgggtta ttcaggaagt cctcgtagga cagacggatg ccatcttcca
    21241 actcagtctt atatgtccat cccagcttgg ttgccttgga aacatccagc agcttacgag
    21301 gtgtaccgtt cggcttgctg gtatcccaca gaatctcgcc ttcgtaacca ataacctttg
    21361 ctaccatctc ggtcagttcc ttgatagaca gttccttgcc agtacctgca ttaacagtct
    21421 cgtcaccgct atagttattc atcaggaaca cacacaggtt tgccagatcg tcaacatata
    21481 ggaattcgcg cagaggagaa ccatcacccc agcaggtaac agtaggcaga cctgcttcct
    21541 tagcctcatg gaagcgacga atcaaagcgg ggagcacatg actgtgagta ggatgatagt
    21601 tgtcgttagg tccatacagg ttagtaggca taacagagat atagtcagta ccgtactgct
    21661 tgtttagaaa ctcacagtac ttcaggccag aaatctttgc cagtgcataa gcctcgttgg
    21721 tcttttccag ctcacttgtc agcagacagc tttccggcat cggctgcggt gccatacgcg
    21781 gatagataca ggaagaaccg aggaactgta acttcttaca gccattctcc catgcggagt
    21841 tgatgacgtt catttccaga atcatgtttt catacatgaa gtctgccaga gcgttctggt
    21901 ttgcgataat accgccaacc tttgcagcag cgagaaatac ataatccggc ttttcctgtg
    21961 cgaaaaatgc ttcaacagcg tcctgacggc acaggtcaag ctccttatgg gtgcgggtga
    22021 taatattgtt gtagtcctga cggttcagct cgcgcacgat tgcggaacca gccatgccac
    22081 gatgaccggc tacgtagatt tttgcgtctt tttccatcat aataatgctt gctcctttct
    22141 aaatttagag catctctcat tgcatgagag aaatcaattt gtctgcgatt tcgctcgcag
    22201 catcataaaa tgtatcaaaa tttgaggtgt ctagctgttt gaggtcctca atcacccgct
    22261 ctacgcggtg gaggttttcc ggtgtgacgg aaattgccag acccagtttt tgcaatttac
    22321 aaatagttgt tctatcttcc aaaactgtat cgcgggcgaa cagtgccatc ggacgctgtg
    22381 cgagaataca ctccgcaaca gttccccatc cagcctttgt aataacgtaa tctgccgccg
    22441 ctatatagtc ctgtgtattg ggggtgctgt tagaaataac ggttacgttt tctcccacaa
    22501 gcggaacacc tagcgttgta atgaaatcat acggcgcgtg actgacatca atcggctgtt
    22561 tgaaccgcgc ggacataccg agtgcgacaa atacaggcgg tttttcatgc tgtgatttaa
    22621 tgcgttgcac ttcgtcgtta tgaatcggac gcgccacaac ggaagtcggt gttttttcgc
    22681 tgttttggag aaattccaac atttcttcgt tatttagcgc ataaagcatg gtgtgctgaa
    22741 ttttaccgta acgcttggca tattctttcc aaatatgttc gggtaaaaat tcgcgataca
    22801 gctctgtcca agtgaagttt cctacataca ggaaaggtac tcctgccata tcgcaggcat
    22861 ccattgccca gattggcata tcgcaaagca ctgcgctgac agcttctcgc ttgagccatt
    22921 cggcttctgt ctttgccaaa gaaggaacct ttgccaaaaa atattcacag atctcggtca
    22981 gcttacccgt gtccaccagt agggtgccct cacggttaat cagaccgata tctgtgttta
    23041 ttggacgata gcgcacgcgg tctatcagct ctttgggcag ttcctgctcc aaattgcgct
    23101 ttgcaaaatc cagatgattt gcgccgcaaa ccagtgtaat cttcgcttcg catcggcaga
    23161 gtgttgccat aataatgggc agattccgcg cgatatgtcc gaagccgtga ctggaaagat
    23221 agaaacagat atgtttattc aatgtcagct tacttgactg cgttcttcat tgcagcttcg
    23281 cgctttgcca attcacggtc atgctttgcc atgatgttaa ccagttcctc gtaagaggtc
    23341 ttctgcggat tccaaccgag aacggtcttt gccttagtcg ggtcgcccca caggttgtca
    23401 acgtcagtcg gacggaacca ctgtggattt acacaaacga gcatcttgcc ggtttcaaca
    23461 tcgtagccct tttcatcaat gccagtgcct tcccaacgaa tggtcatgcc atttgctgcg
    23521 aatgccttct cggtgaagtc acgaacagtg tgctgctcgc cggtagcgat cacgaagtcc
    23581 tcgggagtct catgctggag aatcatccac atgcactcaa catagtcctt tgcatagccc
    23641 cagtcacgca gggagtccat gttgcccagt tccagatggt cctgcaggcc ttctgcaatg
    23701 cggccggcag cgagggtgat cttacgggtg acgaaattct caccgcgacg ttcggattca
    23761 tggttgaaca gaataccgtt gacagcgaac atgccataag cttcacgata ttccttagtg
    23821 atccagaagc catactgctt tgcaacagca taagggctgt aaggatgaaa cggagtggtt
    23881 tcacgctgag gaacttcttc aaccttgcca tacagctcag aagtagatgc ctgataaatc
    23941 ttagtcttct tcgtcaggcc cagaatacgg caagcttcca gaatacgcat aacgcccaaa
    24001 gcatcaacat cgccggagta ctcaggaaca tcaaaagaaa cctgaacatg gctctgtgca
    24061 gcgaggttat agatttcatc aggctgaaca atgctgatga tacgaatcag agaagatgag
    24121 tcggacaggt caccgtcatg aagggtgatc ttgcccatca aatggtcaat gcgagcggta
    24181 ttggaaatag aagcacggcg agtgataccg tgaacttcgt agcccttctc tagcaggaaa
    24241 tctgccagat aggaaccgtc ctgaccagta ataccagtaa tcagcgcttt tttcatgata
    24301 cattatcctt tcctatgtaa gtgataaggc catataaatg gctcgtaatt tctaatactt
    24361 ttcaagaata cacgataatt ttcgatattt catagaacaa tctacgcacg cattagcaca
    24421 atattagttt ttcgtgaatt ggaatggcaa cagttttttt gacaaatttt ataaggtgca
    24481 gaacttcttt ccgtatgcta ttccgattgt ccttgacaat gagcctcctc ggatgtgacg
    24541 atcttcggca ggagctaaca gttaaagtta gactgcctcg ctagcgttag cacatccgag
    24601 ctcattatca aggattcgct gcgccaatct ctggttacgg taaccaaccg gtgtctcgta
    24661 gccaag
//
LOCUS       DACDXO010000037        24576 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E23605_2017
            SAMN12639989-rid9881463.denovo.037, whole genome shotgun sequence.
ACCESSION   DACDXO010000037 DACDXO010000000
VERSION     DACDXO010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639989
            Sequence Read Archive: SRR10023389
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24576)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24576)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741914.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:42:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,972
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 33 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 17 of 73
            Pseudo Genes (multiple problems)  :: 10 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24576
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639989-rid9881463.denovo.037"
                     /isolate="E23605_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(153..551)
                     /locus_tag="IWM35_001687"
     CDS             complement(153..551)
                     /locus_tag="IWM35_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HAP9919537.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            complement(548..1321)
                     /locus_tag="IWM35_001688"
     CDS             complement(548..1321)
                     /locus_tag="IWM35_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HAP9919538.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            complement(1318..2001)
                     /locus_tag="IWM35_001689"
     CDS             complement(1318..2001)
                     /locus_tag="IWM35_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HAP9919539.1"
                     /translation="MLKNFKEVIAAILPMTVLIVILTFVFAPLEGEDLASFLVGAAIM
                     MIGMTLFLFGADYSMMEVGDLVGKYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQ
                     QVYDISDGEIGRVLLIGIVSVGTGVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFT
                     SSEFMPIAFDSGGVTTGPVTVPFILALAGGMTSMIKQKKNENDSFGMVGIASLGPILA
                     VMILGVIFQ"
     gene            complement(2168..3055)
                     /locus_tag="IWM35_001690"
     CDS             complement(2168..3055)
                     /locus_tag="IWM35_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP9919540.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(3052..3921)
                     /locus_tag="IWM35_001691"
     CDS             complement(3052..3921)
                     /locus_tag="IWM35_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP9919541.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(4058..5344)
                     /locus_tag="IWM35_001692"
     CDS             complement(4058..5344)
                     /locus_tag="IWM35_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP9919542.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            5617..6195
                     /locus_tag="IWM35_001693"
     CDS             5617..6195
                     /locus_tag="IWM35_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAP9919543.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPLKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            6195..7124
                     /locus_tag="IWM35_001694"
     CDS             6195..7124
                     /locus_tag="IWM35_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9919544.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            7121..8857
                     /locus_tag="IWM35_001695"
     CDS             7121..8857
                     /locus_tag="IWM35_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP9919545.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(8920..9756)
                     /gene="pheA"
                     /locus_tag="IWM35_001696"
     CDS             complement(8920..9756)
                     /gene="pheA"
                     /locus_tag="IWM35_001696"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HAP9919546.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(9753..10262)
                     /locus_tag="IWM35_001697"
     CDS             complement(9753..10262)
                     /locus_tag="IWM35_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HAP9919547.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(10255..11550)
                     /gene="aroA"
                     /locus_tag="IWM35_001698"
     CDS             complement(10255..11550)
                     /gene="aroA"
                     /locus_tag="IWM35_001698"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HAP9919548.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(11563..12654)
                     /locus_tag="IWM35_001699"
     CDS             complement(11563..12654)
                     /locus_tag="IWM35_001699"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HAP9919549.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(12671..13837)
                     /gene="aroC"
                     /locus_tag="IWM35_001700"
     CDS             complement(12671..13837)
                     /gene="aroC"
                     /locus_tag="IWM35_001700"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HAP9919550.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(13839..14903)
                     /gene="aroB"
                     /locus_tag="IWM35_001701"
     CDS             complement(13839..14903)
                     /gene="aroB"
                     /locus_tag="IWM35_001701"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HAP9919551.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(14936..15958)
                     /gene="aroF"
                     /locus_tag="IWM35_001702"
     CDS             complement(14936..15958)
                     /gene="aroF"
                     /locus_tag="IWM35_001702"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HAP9919552.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
     gene            complement(16617..17591)
                     /locus_tag="IWM35_001703"
     CDS             complement(16617..17591)
                     /locus_tag="IWM35_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HAP9919553.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
     gene            complement(17572..19455)
                     /locus_tag="IWM35_001704"
     CDS             complement(17572..19455)
                     /locus_tag="IWM35_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HAP9919554.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            complement(19473..21698)
                     /locus_tag="IWM35_001705"
     CDS             complement(19473..21698)
                     /locus_tag="IWM35_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HAP9919555.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEKKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            21813..22847
                     /locus_tag="IWM35_001706"
     CDS             21813..22847
                     /locus_tag="IWM35_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP9919556.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            complement(22914..23927)
                     /locus_tag="IWM35_001707"
     CDS             complement(22914..23927)
                     /locus_tag="IWM35_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP9919557.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKQLLPKDSLKGTISYCGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(24005..24193)
                     /locus_tag="IWM35_001708"
     CDS             complement(24005..24193)
                     /locus_tag="IWM35_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HAP9919558.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
BASE COUNT         7062 a         5341 c         4397 g         7776 t
ORIGIN      
        1 agcatatttt gaaaagaaag tctgatgctt agagtagaat ttcataaaaa tttactattt
       61 cataagtatt cacgagaaaa atacaaacaa aaaagcggaa ataacgctca tccgccttct
      121 tgtttgtgtt tatttcctct tcaaacgctt gtctatgatt cagacttttc tctatcgtta
      181 ttttcatcta aaagttcttg cattccgata atttctcgat attgatgaat cttagtgata
      241 ccaatgactt tgtctacatc aatactaaaa caaataccat tacctggaat atcgatgttg
      301 atgccttgac caatcgcatc cattacttta ttcgtcaaac tatcgggtac taatgtcaac
      361 acaatatctt tttctggcga aatgtccaaa cctaaaaatt tccctgaatc atgtacacca
      421 gaacctcttc catgtaagat cgttccgcct gctgcacctg cttcttttga aaagtcgata
      481 acatcttcgc ccattcctcc gtcaacgatc gtcacaatca tgtgaagatt caatgctaca
      541 agatctttca tttgctttcc cccaattttt gcaatctttc tttttcgatt ctgtcttttt
      601 catcttttcg tttataaata tatcctagta ctaaagtgga taatattggt gtcaatgcaa
      661 tcattgcaac cacaccaaac ccgtcaatga tcggatctct tccttcaatt tgtgcagcga
      721 ttgccacgct cattgataaa ataaatgtcg aacacatcgg gcccgttgct actccaccgt
      781 tatcaaaagc cattgctaaa aagatcttgt ccacttttcg tcctaaaata aatgctagcc
      841 cgtatcctgg gatcaagaaa taataaagag agaatccgac gagcaaacgc cacatagaca
      901 atcctactgc tgcgccgatc ccaattgaaa ttaccatcag catgattttc gatcggattc
      961 tcccctcgct caattcttca acctgtttga tcattacatg aatcgccggc tcagcaaacc
     1021 ctactaagaa ccccattaaa aagccaaggg gaaccagtat ataattgtaa ggcaatcccg
     1081 caatagcact gcctaaatgc tgtcctaccg gtacataagc catattgact ccgtgcagga
     1141 aaaggatcaa cccaatagtc gttatcaaga aacctttcat aatgtcaaag aaacgctttt
     1201 tgcgcatacg aaaactaatg atattcatta aaacaaatag gattacgata ggtgccactg
     1261 ccaaaatgac ttccatggta acgatttcga ttccatctat cacttgctgc cagaaactca
     1321 ttggaagatc acccccaaaa tcatcacggc aagaatcggc cctaacgaag caataccgac
     1381 cattccaaaa ctgtcatttt catttttctt ttgtttgatc atactagtca tccctccggc
     1441 aagggccaag ataaatggaa cagttactgg accagtagtc actccccccg aatcaaaagc
     1501 aatcggcata aattcggaac tggtgaaaaa tgaggcgatc aaaacgccta tgtagcctat
     1561 agccatcaat tgataatagg atagcttgaa tacgacccga agcagcgcaa aggctagaaa
     1621 tactccagtg ccgacgctga cgatcccaat cagcaacact ctgccaatct ctccgtcaga
     1681 tatatcatat acttgttgtc ccaaaacttg cacggaaggt tcagcaatcg tgatcacgat
     1741 cccgatagca aatcccaaac tgaccaaaac ggtcaaactt ttctttttga tcatatattt
     1801 ccctactaga tctccgactt ccatcataga ataatctgct ccaaacaaaa agagcgtcat
     1861 tccgatcatc atgattgccg caccaaccag aaatgaagcg aggtcttctc cttctaacgg
     1921 tgcaaaaaca aacgttaaaa tgacaatcag gacagtcatc ggcaatatag cagcaatcac
     1981 ttctttaaaa ttttttagca tgtatcctcc tcctttactc tttaagttta ccttaaaatg
     2041 aaagagtttt cgagcacaaa cttaaaacgt tatcatttaa tttttctcaa cataaaataa
     2101 cacacgtccc aaaagtatct ttttacttgg aaaaatgact ttgaaggaac gcgcttattg
     2161 tgttttttta ttttacagag ccgatcattc cttgaacgaa ttgcttttgc aaaacaaaaa
     2221 atacgatggc agttggtaaa gtagcgatga ctaatccagc catgatcatt ccatagtctg
     2281 gtgtatagga agcacccata gcagataaaa caagcggaac cgtccgttta tcaggagatt
     2341 gcaaagctac taagggccat aaataattgt tccagctact catgaatgtg atgattgcag
     2401 ccgcggcata ggtattttta gccgtcggca tatacactct aaaaaaaata cttagttctt
     2461 ttagaccgtc taatcgagcg gcttctacta gatcttttgg aaaagattta acattttgtc
     2521 gaaagaagaa gatcaaaaaa gcagtgctaa cagatggcag gatgacaacc aagaaactgt
     2581 tgattcccaa aaccgttccg ttcaattgtg aaaacattcg atataaaggg atcatcaatg
     2641 ccgcaaaagg aaccatcatc gatagcagta gaatattgaa aattaggtct tttcgtttac
     2701 tttgataaat ctcaaatcca tatcctgcca ttgacgaaat cagcaatgca aacacagtgg
     2761 tcaccaaagc gatcatcgct gaattccaaa gggaacgagt aaaattcagc tcattggaaa
     2821 acaaattttg aaaattgatg atcaagttac tgccaatctt tatttttccc gatgtgatat
     2881 ccacgggtgt attcgtcatt cccaaaacca tccagacaaa agggaaaatg gaaataaatg
     2941 ataggatgct taatgctgta tactttaata ctgacccaat tgtcgaacga agttctaatg
     3001 cttgtttttt gacgttgggg cgttcgattg tttctttttc taccatattt ttcatcgact
     3061 ttctcctcct actttcgttt ggattatcga caatatcaca ataaaggaaa caataacaaa
     3121 ggaaactgct gccgcatatc caaaattagg cgtgaattta taactcaaat tatagatgta
     3181 ttgagatagg gtggtcgttg catttgctgg tcccccgcca gtaatatttt gtacttcatc
     3241 aaaaagctgc aatgtcccga tcgtcgaagt aatggaagta aacaaaatga ttggttttaa
     3301 atttgggatc gtcactttga taaactgctg ccatttagaa gcaccgtcaa tagatgctgc
     3361 ttcataaatc tccgaatcaa tattttgcat gcctgaaagg aaaaaaatca tattgtatcc
     3421 agtccaacgc caagtgatcg aaagaatgat cagaactctc gcccaaattg gatgagtcaa
     3481 ccagccaatc ggtgtgtcaa tcataccaat gcccaacaac aagttgttta caaggccaga
     3541 ttgagaaaat agacttttca tgatcagaga ataactgacc atcgaagtga tacatggaag
     3601 aaagatagcg gttcgaaaca accctttata tttcaacttt ttatcattta aaaggttgga
     3661 aataaaaagt gctagaaaca acatgatcgg tacttgtaca agtaaataca ggaatgtatt
     3721 aaaaaaagct tttttaaaag ttgcatctgt caacatccgc tgataattac ctaacccgtt
     3781 aaatgtcata ttgacagggg gaccactttg aaaagacgtg atcaaagcca aaaccatcgg
     3841 aataaaaacc atgattgata tcaaaatgac tggcaagatt aaaaaagcat gcccgttttt
     3901 gaataaatct ttctgcttca ttcttttccc tccaattcta tcattgatta tctacatgaa
     3961 aagacaagaa agacaatatc tgtaaggcag acgtgatctt ttttgtcttt tcgtttgtct
     4021 gatgaaaaag cgaagaaatt cactttttta ccaccgatca attagccaat tgtgcttcta
     4081 cttgtttttg tgtatctgct agtaccttgt ctgtctcttc tccattgatg attcgatgaa
     4141 gagcttcagc tacgactgct tcaattgcat aggtctcatg tccgtaattc acttctggta
     4201 tttcagatgt ccatgttgaa aaatcgtcaa agatcttttg accactgtaa aattcagaag
     4261 cttcttgata agcttcttgt tcattcgcgc taagcatcgt agatactagt ccgatttctt
     4321 tcgcaagagt gcccatcagt tcttcattcg aagcaaacgt tgcttctagg aaatctttcg
     4381 ctgaatcttc tccagctact cctttgatca cataccagcc agcgccgcca aggttagaag
     4441 cttgcgcttt ttgcagattt tctggaagtg caggtattgg tgcaatcttc cattttccag
     4501 attggttttc tgctccttgg atcgttgaag aataccaagc gccagttggg ctagaagcta
     4561 ctgctcctga ttgtatcgca gttactccag cattccagtc agaggtttgt tcgactagat
     4621 cttctttcaa aagagtggca aacaattcaa gaccatactt taaagatgca ttgtcttgga
     4681 tcgtgactgt ctccccatct tcagatgtat accattctcc tgcctgttgc ataatcatcc
     4741 ttactcgacc aaggtcagaa ggattgactt cagcgatttt ctttcccgtc ttttctttga
     4801 catctcgcgc tacttgaata tactcgtccc acttcagatt ctccatatcc tcttctgtat
     4861 aacctgcttc tgccatcaga tccgtacgat agaagtttgc tgttacgccc gaatcgaaag
     4921 gaacaccgta tattttatct cctactttgt ttaccgcaaa tttatacggt gcaaaatgat
     4981 cttcctttac aatagcagac aaattagcga atgcatctgg ataagaagtc aaatagcctt
     5041 gtatcctata atcttcaatc aagacgatat tgggcaatcc ttctgtattc ccgctcgcta
     5101 aagcggtgtt cagtttctga acgatatcat cctgagacat cgtaacaact tctatctctg
     5161 tttcttcgtt ctcataaact ttttttgctt cattcactgc tttgatattg aatgtctcat
     5221 cccacgccca gacagtgact ttttcttctt tgtctgctgt actactagca cttccgcatc
     5281 ctgttaagaa acctgatgct gccgctaaac aaacgactcc agcaattaac tttctgatgt
     5341 tcatttttat ccgctccttt tccatccatt agttgtttac gtttgctaca ttcatcatac
     5401 caaaatttga aaacggtttc tttattattc agatacttca aaaatttctc ctaaaataat
     5461 aaaaaaatac aaatcaacta tgacaaaagg aataatctta gcatctagac caaagatcga
     5521 aatggtgagg tttctttaaa ataaaaatat ccttgcattt tctcctagaa ataagtattt
     5581 ttaaattaca agactattct taaggcgggt aaaaaaatgc caatacgcgt aattcttatc
     5641 gatgatgaac agacgatttt agatggaatg aaacagttga tcaattggtc tgagcacggg
     5701 tttcaattaa tagggacttt ttccagtccg ctgaaagcgc ttgatttttc tttggtacat
     5761 gcaatcgata tcgtggtcac agatgtaatg atgcctgaat taaatggaat cgaattatcg
     5821 cggctattaa aacagcaaca acctgaaaca cagatcctta tcctctctag ctatgatgaa
     5881 tttgatatgg ttaaagattc ttttaaagaa ggcgtcgctg attatctttt aaagcctcga
     5941 ttgagtcctg atttttttct aaattcctta actgccgttt cacaaaaaat caaaaaaaag
     6001 cccaacaaaa gcccaatcac caatcgttat gagcagatct gcgagaatct ttcacagcag
     6061 attgctggac aaactcgtcc attgcttgac cctcttcttt ttgaacacac cttgtttttc
     6121 tgttatatac cgaaaaacgg ccattcagtt caaaacaagg ctctaaacaa aaacagtcga
     6181 tcacccattc attgatggat agcgacgatt cgtttactat ttatcctgtt tctacctttt
     6241 cggaagaatc cggctatgta atcaatactg attccaaaga tacacttagg aagtttgttc
     6301 aaaagtttcg tcaagaagtt tttccaggtg atttttttgt tgtttctgat tcttttgcaa
     6361 tccaagatat caatgaaata ttccaagaga tcagaaattg caccaaagga caacgtttct
     6421 atcataaggg aaagaacctt gcttatcagc atgaacttct tccgttatta accaaccacg
     6481 agcagcaagc aagtagttat ttaacaaaaa tcgttcataa agactacgct ttagctgtca
     6541 aagaacttga gaatttcttc acgacgatat tggaacagcc tattgatcca gcttatttga
     6601 aacatgaggc aatcaatcgt ttctatgcat tactcaacgc tttggctgac gagcatgttg
     6661 ggaatgaaga actttctttg ctcaagatcg cccttccttc tttattagcc gatgctgagt
     6721 atttcgaaga tttttctatt cttgtcaaag aaacacttga gaaactcacc cacttttctg
     6781 caaatgcaaa aaaagacaat ttcgatatct tgactttgat ctacgagttc gtccaggaaa
     6841 attatgagaa agaaatcaat ttacaacttt tatccgaaaa atatcatttt tcatatagct
     6901 atctctcagc catctttact gaaaaatttg gtatcaactt ttcaaaatat ctaaaaaaag
     6961 tccgtatcag taaagccaag ctctttttga cggaaacacg gcttactttg acggaaatct
     7021 gtgagaaagt cggctatacc gaactgggtt atttctccag agtttttaaa gaagagacag
     7081 gaatgacacc ttctcaatac cgtaaaggaa cttttgtaaa atgagaaaaa aaatcaaaga
     7141 gcatgaactc tttgccaaac ttctgttcat tttgttcatc ggtctttttc tgcaagccat
     7201 cgtcatttct ttgtttattt atcatcgatc aagggatgcc tatatccagc tgttcaatca
     7261 atcgaatgat gtggtactaa aaaagattca atctgaattt gaaagtttga atgatacaat
     7321 cgagaataca ttagccgctt ttgattctaa tccagctgtc aaaagctatt tttccaatga
     7381 tccagcccaa catatggaac agcttcagca actaagaaca atccaaaaaa tgaatgactc
     7441 tctttcaaaa atccatccaa tgatcgatta cgatgtattg atctttggag aaaatggaag
     7501 aacttttgtc ggaaacgaca tgctcactgc cgtatctgcc gattcttttt ttcaatctgc
     7561 gattgctcag agagtgaatg aacgtgctgc tgatacacaa atgctttttg cacaccacgg
     7621 attgactctt cgcgataaga aagcacccag cgtcttcttt gtcaaaaaac tgaaaaatac
     7681 attgaatcac gtttacggat atgctgtttt atcgatctca tccaagcaac tagcgaatct
     7741 ttttcaatct gtagtaaatc ctgagatatc gaagatctcc ttcatgaacg aagaacagac
     7801 gatcatcgca tcaaatgaag aaaatacaat tgggagccga tcaatgattt tcaaaaaact
     7861 aacagtaggt gaaacaacga tttcaaatgg acagcagttg acccgtcttc ctctgtaccg
     7921 ccaggattgt acattactca gtcaagtcga tcttcattct ttatctaacc aaatgggcgt
     7981 gatttttccg attattttgt ataatatcgt gacacttgct tttgctggct cattggtatt
     8041 tatgtatttg gatcgacaca caaaatcgat ctaccgcttg attgattcgc taaaacggct
     8101 agaggaagat ggcttaaaag ctactgttcc tgtacaagga agccatgaag tccaattgct
     8161 agggaatacg atcaatcaat tgctaagtaa tgctcagcat cattatgaac agacgatcca
     8221 agacgaaaag aagaaacgtt cgcttgaaat ccaaactatg caagcacaga tacagcccca
     8281 ttttatttat aatacgctga ctagcttgaa atttttgatt tggcagaaaa aaacagatga
     8341 agcagttcaa ggaatcgaca gtttcattac tttactaaga agtacgattg ggaaaaaaga
     8401 agaagtgatt ccagtcaagg aagaaatcaa aagcgtacaa agttacatcg atcttttgag
     8461 tatgcgatat ggcgaaagaa tctcggctaa aataatgatt cctgatgaac tgcagtcatt
     8521 atctatgccg aatatgatcc ttcaaccaat tatcgaaaac gcctatcttc atgcttttca
     8581 caataaaacg caaggattca tcacggtgta cggcaaaaca aaaaatcagc ttcttttatt
     8641 cgaagtgatt gataacggcg atggcttcga tactgaaaat accaaacaaa aaaatgatca
     8701 tttttcagga atcggcatca gccatgtaga tgagcggata cgcctaatgt atggcgatgc
     8761 atatggctta acgatccaat cagtgattgg gacaggaacg actgtaacga tttgtctacc
     8821 gcttcttcct aaaaatcaat cagaatatca aaaataaaga atagccaagc gctgattttt
     8881 ttattttaag ttaaataaat caatcagtat tcttgacttt catctacgcg tctcttctac
     8941 tgtcagcact ggataacagc ccaatatttg actttctcct ccgagcatct tgatttcttg
     9001 gagcgcattc tctactaatg tcattggacg gtccaacaac aaatcgatca cgaaaaaata
     9061 ttctcccaag ctagttttca atggtcggga ttcaatctta cttaagttga tttcccgcca
     9121 gccaaaagct gccagcattt tatgaagcat ccctggccga ttagcaggta gtgtcaagat
     9181 gacactcatt tttactggag ctgactgctg gctggtcatc tttcggtcac cgacgatcca
     9241 aaaacgtgtc tggttcaact cattatcttg gatattctca gctaagatct ccaatccaac
     9301 atgctgtgct gtttctaatg aagcaatcgc tgctgcgtct tctttcggat gttctgctac
     9361 atacattgct gcagcagttg ttgattctgt tgctactaaa ggaacattag gataatgcgt
     9421 ttctaaaaac tgttgtgatt gtgctagtgc ttgtggatgt gaaaggattt ttgtaatctt
     9481 tgttgctgga ttgcctaaaa gctgctgctt gataggaaga acgatttcac ttttaacttc
     9541 gacttctgga tgtttggaaa gaagatcgat tgtgtgatga acagctcctt ccaatgaatt
     9601 ctctacagga acgacagcca gatcgacttg tttgcgaaac aaagcgcgca aacaaacagg
     9661 gatggaagca tacgcagtca attgttctgt aggaaatagc tgacaagccg cctgataagt
     9721 gaatgaactt tctggcccta gatagctgac gttcatatgc ttttgatcct ttctataatg
     9781 gtatcgatga tcttttctgg tgtttgattg gtcgtgttga ttcgatgtgt cgcactttct
     9841 tcataccaag aaagtcgttg tgccaataat gcttttattt cttctcttgt ttttccaaga
     9901 gctaaaggtc gaatagacgt ttcatcttgt tggatacgat caactagaca gtccgcttct
     9961 gcctctaagt aaagaacaat cggctgtgtt tttaaaaaac gccgattttc tgcactttgg
    10021 acaatccctc caccagtcgc gataattttg tcttcatttg acagctttct taaaatatct
    10081 gactccactt ttcgaaagct tttttcaccg taatagtgaa aatattcggc aatcgacaat
    10141 tggatatgtt cctcaatctt tgtatctaaa tctacatact ctttttgtaa acattgagct
    10201 aaccctttac cgattgttgt ttttccagcg cccatgaaac caatcagcaa aatgctagcc
    10261 atgagcttct cctcctgaca caagttctgc taaatcgtca aaaaatgccg gataagagat
    10321 tgaaacagct tcagctcctt ctagttcgca aggtccatca gctatcaaag cagctacttg
    10381 cagcatcata ccgattcgat gatcgcctct gctggacact tttgcgccat gcagaggtgt
    10441 cggcccatgg atgatcagac cgtctgctgt cgcttttatc tttgctccca ttttctgtaa
    10501 ttcttctgcc accgcatcaa ttctgttggt ttcttttact tttaattctt ctgcgtcttt
    10561 gatgatcgtg attccttctg cttgagtagc cactaaagct agaatgggca attcatcaat
    10621 cagacgcgga atgatttctc caccaatcgt tgctttcttc aaatgactcg tcttgacact
    10681 taaatcagcc gattggttct gttcattgtg attcgtctgg gtaatcgctg cgcccatctc
    10741 ctccaacaca tctaagatac cagttcttgt tggattgacc ccgacatttt ttaacaacag
    10801 ttggctttcc ggaacaagaa gtcctgctgc tagaaagaaa gcagctgatg aaatatcacc
    10861 tggaacagtt atctgttgac cagttaattt ttgcggtccg gtcactatga tcgttttatc
    10921 ttccactgtg atccttccgc caaattgacg aatcatttct tccgtatggt tacgggaacg
    10981 ttctttttca acgattacgg atgtgccttc tgcttgtaat gccgcaaaca gcaaagcaga
    11041 tttgacttgc gcactggcta caggcatatt gtatcggata ggtgataaag agcgggttcc
    11101 tgtcacagtg attggtagat actggtcatt ttcttctcct tgcaagtcag cgcccatctc
    11161 gcgtaaaggt tccatcactc gccccattgg acgtttagac aacgaagcat ccccgaacag
    11221 agtagtcgta aaaggctgtc cagccaaaat acccatcaat aaacgtatgg tcgtaccgga
    11281 gtttcccatg tcaattgatt cggttggttg gacaaatgag tggctaccgc gaccatgaat
    11341 cttgatcacc tcttcctctt cttcgatctc gacacctaat tgttggaagg catgtaaagt
    11401 actcagacaa tcttctgcgc gcaaaaaatt ttgtatagtc gtcgttcctt cagcaattgc
    11461 tccaaacatg atgctgcgat gggaaattga tttatctgct ggtatcctaa cagtccctct
    11521 taatccatgt atttgttgta ataattgcat ttatccagcc tcctatttga tccagcaatg
    11581 ataactggta ttagacataa tgattctttt tgcttgtaac aaatcctgtt ggtttttaaa
    11641 actgatttgc agtattccta aaatatcttc cctggtctct tggatcttca aattaatcaa
    11701 agaaactccc gcttttccga tcagtccagt tacttctgca ataacgccag gttcatctgg
    11761 cacatcgata aataaatcaa aaaaggcagg aatcgcgcct tgcttatgga caggcaactg
    11821 gtctcgactt tcttttgccc gattgaaaaa agaaaagatt tccgattggt tctcagtcat
    11881 gatccattct gctatttgtg tcatttcttg ttgccaatct gccaatcgtt ccaataatat
    11941 ctgcttattg ctcaacaaaa tatctgtcca catgacagga tctgaggaag cgatcctagt
    12001 gatatctcga aagcctcctg ctgccagttg tttggctcgc ggatactctt gattgaattg
    12061 ttctgcctga ttgactaagc cagctgccag aatatgtgga aaatgactta gcattcctgt
    12121 tatacggtca tgttctcttg ctgtaagagt tacatatttt gctttggttc cgcaaaatag
    12181 ttcttgcagt ttttcaacat cttttttcgt cttttccaaa ggtgtaaaga tgtaataggc
    12241 attttcaaat agtttagcat ctgctgcctg tatacctgat ttatgagaac cagccattgg
    12301 atgtccaccg acaaaccgaa gatttttctg ctcagcggat gtcacgactt gctgcttcgt
    12361 actgctgaca tccgtaatga tgacgtgatc agacaagttg attctttcaa ggacatcaag
    12421 ataagctaag gttgtacgga cgggaacggc taataagata acgtctgctt gttctgcacc
    12481 tgtttcaaaa tcacttgcaa cgtaatcaac gatctgctgt tctactgctt gctcacgggt
    12541 ttttgactga ctatcccaac caactactat cagttctgga ttggtctgac gaatacaaag
    12601 tgccatagaa gcaccaatca gtccaagccc cacgataaaa acatgttgtg ccatcttatt
    12661 tgcccccttt ttagtaatgt ttcaagtagt cacggtactc cttcactgca gctttcaact
    12721 caacaagcga atcacttggg aattttgaca atagctctgt cgctaccaca gtggctacaa
    12781 cattttccgc aacgacgcta gctgcaggaa ctgctgtact atccgaacgt tctacacttg
    12841 ctttgtaagg ttcttttgta tcgatatcaa cactttgaag tggcttgtac aatgtaggaa
    12901 tgggtttcat gaccccgcga atgacaagtg gttcgccatt ggtcattccg ccttcgaagc
    12961 cgcctagatt attggtacgt cggaaatagc ctctttcttc ttcccatagg atctcatcca
    13021 tcacttctga gcctggtaat gaaccagaag tgaatccgac cccaaattct acgcctttaa
    13081 aggcattgat actaaccaca gcttgtgcga ttttggcatc tagcttcgtg tcccattgta
    13141 cgtagcttcc taatccagct ggaacgcctc caacgagcac ttctacaatt cctccgatcg
    13201 tatctccatt tttctttgtc tgatcgatca aatcttttat tctttgctca actgctggat
    13261 caacaactcg tacttctgac gcttcagaaa tttctttgat ttcttgaatc gagatatttc
    13321 caggtatttc tgcttggatt ccaccaagac aaatcacgtg tcctgctacg atgatgtcaa
    13381 gttcttccag aattttcttg gcaattgcgc cgattgctac acgcatggtc gtctctcttg
    13441 cagaggaccg ttcaagcaca ttccgaagat ctcgatgatg gtatttcata ccgcctacca
    13501 aatccgcgtg tcctggacgc ggacgtgcaa ctcgccgcag tgattggtct ttttcaggca
    13561 cttctgctac agacatgaca gttgtccaat ttttccaatc tttattttct accactaaag
    13621 tgacaggtga accaattgtt ttgccgtgac ggatgccaga cgtgatccga acacgatctt
    13681 tttcgatctt catccttcct cctctgccgt aaccagcttg tctgcgagcg agttcgtgat
    13741 tgatttcttc ttctgacaga ggtaatcctg ctggtagtcc ttcaataatg gctgttaatt
    13801 ctggtccatg ggattctccc gcagtcatat aacgcatctt acagtcctcc taagtaatct
    13861 ttgaatgtct ctgtgggaac aggaacgatt tttgcttgcc caatcttttc tagcaagatg
    13921 atattcaatt ggcttcctct tgctttctta tcatgggtta tcgcgtggaa taattcatct
    13981 ggatgactta gatcgtacgt aatcggcaga tggaattttt gaagtatcct ttctaaagca
    14041 gctgtcgatc cagttggaga caagccgatt ttttctgcat gagaagtgat ggctaccatt
    14101 ccaatagcta ctccttctcc atgactgact accccatatc ctgctgtatt ttcaattgca
    14161 tggccaattg tatgaccaaa atttaaaata agtcgatttc cttggtcaaa gacatcttct
    14221 tccactactt ttcgtttgat ttccaacgta gcagtaatga ttggaacagc atgttccaga
    14281 agatcttttt cgtctttcag ctcgttcagc tgtttccaaa gcgttgcatc tgctatagct
    14341 gctgatttga tgatttcagc gattccttct cgtaaacgac gtaccggcaa agtttttaga
    14401 acctcaggat caatcaatac accagctggc tggctaaatg ttccgacaag gtttttcgct
    14461 ttgtttgtat tgactgctgt cttcccgcca atactagaat ctacttgtgc aagtagcgta
    14521 gtcggaattt gaacgaaatg gatcccacgc atgaacgtcg aggccacgaa tgctgctaga
    14581 tcacccacca ttcctccacc aagtgcaagt atcccatcgc tgcgggtaaa atcattatct
    14641 gctaaaaaat cgtataactc agctgcttgt tctaaagatt tactggtctc acccgccggt
    14701 acgattgaaa ggactggttc aaaattagct tgtctcaatt ggaacatgat tttttctgca
    14761 tatagtggag caacgtttgt atcagtgatc acagctactt tttgaggtct ccaaatcgtt
    14821 tgaagccatg atccagcctg ttctagtgcg cctttttcaa tatgaatatc gtatttttgg
    14881 ttatcaacat gtacagtaag cattcgtttc acttctttct tttaaaaagc tggaattaat
    14941 cgagtgtctg aatcttttcc attgcttctt tcatgatcgc aacttctttc atcatgcgtg
    15001 agtaagtctt ttcattcaac gattggggac cgtctgacca agcatttgca ggatcaggat
    15061 ggatctctac gatcatgcca tctgctccgc ttgccacacc cgcacgagcc attggtggta
    15121 caagttccca aattcctgta ccatgacttg gatcaacaat cacagggaaa tgagttaatt
    15181 ttttcatcaa tgggacagca cttaaatcaa acgtattacg tgtcgctgtt tcgtaagtcc
    15241 gaatcccacg ttcgataaaa atgacaggag atttgtcttc gactgcaatg tattctgcag
    15301 cattaagcca ttcattgatc gtaccggaaa ttccacgttt caatccaaca ggtttacctg
    15361 ttttaccgac agcagatagc agtttaaaat tttgcatatt acgtgcgcca atctgtagga
    15421 tatcgctgta ctcagccacc atatcgatat gcccttcatc catcacttca gtaatcactt
    15481 tcattccaaa ttcatcagcc gcttgacgga tgtatttcaa tccttcttct tctaaacctt
    15541 ggaaagcata tggagaagta cgtggtttga aggcaccacc tcgcagaatt tttgcgcccc
    15601 cggctttggc cattcgcgca cattctctga tttggtctaa tccttcgata gaacaaggac
    15661 cagccatcgt cacgaaactg ccgtcaccaa tttttactcc gtctacatcc acgactgtat
    15721 tgccaggatg gaattctcga gaagtcaatt tgtatgtgtt taaaatacgc acagcatttt
    15781 ctactccttc atagcgtgta aatgctcctt cttgtaaact tcgcgtatca ccttttagtc
    15841 caatcactac tcgttctgtt ccttgatcga ttgagacttt gaatccttct ttttcgatat
    15901 gatcgacgac tttttttatt tgttcttccg ttgcttcttt tttcataatt actatcattg
    15961 tttttcccgc tttcttttgt ttacttatct atcttggctg attttctcta ctttttcaag
    16021 gtggttttac attaattcct actaaatgac cgtgtcctgt tgttctcatt ttcgctcctt
    16081 ctttcctact tatgacccag ataatcaaca aaaaaagatg tccctgtagg aaattgcctc
    16141 tacagtgaac atcttcaacg agccttaaaa agcgctgcaa aaatataaaa tagccactat
    16201 agatgaattt ctctacgtgg ctgtaagtcg tgtttaaaaa tctttagcca tcatagatac
    16261 aaacctggtt tgtttgtacc cacgaagtca tgaatacaaa cctatctaaa atagctaaag
    16321 taatacgtat tcagttttgt aagttttcta gatttcataa aaaatcgctc caaactgatg
    16381 taagaattgt acctagtata gacataaaaa aatcgactgt caatcatttt tatgaaactt
    16441 tttttaattt tctgatagtt aactttgttt ttcagaaaaa taacaattgg ttttattgaa
    16501 gaaaatagtc atgaacaaaa gtatctgaga acttttttac tctcttgttc atgacatata
    16561 gaataaagca gctttgtctt gtaaactccg tttttcaaat aataatctaa agattgctac
    16621 tgaataataa agccgatttc atggtcctct tcgctgctga ttcggtaagg tgactcaaca
    16681 tccgctcccc agctattgat acccccgact cctcttactt ttgcaaaaat cgtaagaacc
    16741 gttcgtcttt ctggcggtag ttcttctata tgtgttgcct gttccagttc ttctgctttg
    16801 aatggtaaag cggaaaaagc aaatggcgta tcctgctgtt caaatcgcaa cgaaaaatct
    16861 tcttgatctt tatccgtatt atccaacgtt tgatttcttg ttagttctac ccatttcgtt
    16921 tccatatgca tgccgcattc ttggggaaca aggtatgggg aaacggggag tcctgccact
    16981 ttataaatac cttcgacacc accagccatc cgatccggat atgtttcccc tgataggcct
    17041 tcatactcaa aaaattttgc tttcgttggc ataatgaacc ttaaacccaa tgcgggtaat
    17101 tccggtaatc ctttttgtcc ataataatgc atattgacaa gcattttacc ctctccagcg
    17161 atttggtagg aaattgtcac ttgggttttt ggtactgtgt tgatttccag tgtaaaaatg
    17221 atttcaaccg tttcagcgta ttcattactt gaatattgat tatttactgg agcaatcggc
    17281 aaatcgatct ttttattgtc caccagtact tctatttctt tgacttttgg atacaatccg
    17341 gctcctaacc acataccgga tttaaatcca aatccgctac ctctgtcatt atcggtcgtt
    17401 gctcgccaaa aaactggaag tgtttcccgg tatagccatt cttttccgtt tttattgagc
    17461 gattccagtc cgcctcttgt ataagaaaaa atataatgcg tattccccga tttgacacct
    17521 aatgtacagt cgccgaagac gattgcaact ttttccgtat ccatatgact tttatttact
    17581 gtatttttca taataatatc ttacctcctg catggcgggt ttttcttgtc catctgcaaa
    17641 aacgatccca ttccctgaga attcatagtc acttggttta tcgtcaaagt caccgccata
    17701 gcgcatcact tctctgcctg tgattggatc agtcacttgg atcgcctgat ctttaaaatc
    17761 ccatatatag cctccttggt acatttcata acgatcaagc aacgtcatgt aagaatccat
    17821 tccacctaac gaattcccca tgtcatgcat atattcacat aagatgaacg gtttttccgc
    17881 tttattttca aggtagtcaa tgatctcttc tggtggcgca tacatgcagc tttttacatc
    17941 tgaaatcgaa tcttcgaatg ctggatttgg aaaattccct tcataatgga caagtcgatc
    18001 ggggtctact tttttgaagt aatcgttcat ttgtcgaatg ttttcttccg cataagattc
    18061 atttccaaga gaccaaaaaa gaatacttgg atggtttttt aatgtttcga aattcgtttt
    18121 tgctcgatca agaacagccg cctgccactg aggtactgca cctggtacgt tgtaagaagg
    18181 ctcaaatgcg cccatttttt gccatgagcc atgggattcg aggttcgttt ccgccatcac
    18241 atggattcct gcttggtcgc ataaataata ccaagtgatc tggttggggt aatgagatgt
    18301 ccgtacgccg ttgatctttg cattttttat gatagccaaa tctttttcca tatcttctat
    18361 agtgatggcg cgtccttttt tcggatgcca ctcatgtcga tttaccccgt tgataatcat
    18421 ccgttttcca ttgaagtaga tgattttatc tttgatttcg attttacgaa aaccgaagga
    18481 atagtcaata tactccgtta ctcttttttc ttcgtctatc gtctccaatt caagcttata
    18541 taaattgggc tgaaaatgcc cccacagtgc cacattttca aatccatcga tgcgaatcgt
    18601 catttgctct tttaacggat attgctgttg atagatttcc ttcccatcag gatctagtat
    18661 cgctacattc agctgactgt ttttggctac ttgctcaaaa gacaaatcta cttcaagttt
    18721 gccattttga catttgtcat ctaagcgagg tttgatccaa agatcttcta aatggttttg
    18781 tcctcgagca actaagaaga catctctgaa gatcccaaaa aatcggaaaa agtcctgatc
    18841 ttccaagaat gcagctgtac ttcttttgta aacctccaca gctagaatat tttcttcttc
    18901 caaataaggt gttaaatcaa attcagacgg agtgaacgaa tcttcggcat acccgataaa
    18961 atgaccattc aaccaaataa acattgcttg ttctacacca gcaaaatgaa tcgagatttg
    19021 ttttcccttt agaccttctt ctaatcgaaa agtcgtcaaa tacgaaccaa ccggattgtc
    19081 tttagcagct gaaaagatcc cttccgcctc tttctcaggg cttgtcgtga aagggggacg
    19141 gcgaaataca tgtccctccc acggatacat cgtattgatg taatggatct tgtcataatt
    19201 tgctaattca atatgactag gaacagtgat ttcatcaaaa gaacaggcat caaaatcttt
    19261 tttataaaat gtttttggtc gtaccgaagg atttggcgaa taacaaaaac gccatgatcc
    19321 atttaaagac tgtttaaggg aggatgtggt ttctacttct tcccttgttg cataacaaac
    19381 atgatcgcta tgtgcatttg attggttcac tctaaaaact tgtggatcat ctaaccattt
    19441 tatatttgct ttcatacttt tttccttctc tctcataact ttttgaaatg aagcagttct
    19501 gtttggaaat caccttgaaa atcggggaag taaagtccaa ctgtcatcaa ttcggccccc
    19561 gaaaatatcc gcttcgaggt ctgttcctga taaagtgcct catcatctaa ccccatcaga
    19621 tatacttcat ggaacgctgg ttgagctgaa gctagtatcc tccctaaaaa cacaatagct
    19681 tcatcttgtt taggagacac aaacatccat gcggcaatat tttcttcaaa aggactcgcc
    19741 aaacgataat aatggccata ctggatcacc ggacgaatcg tttgatagga tataatttgg
    19801 tcttttactt gctttttttc ttcaggagac agctttgtca aatccagttc ataaccaaaa
    19861 acagcgctca tagctgtttc tcctcttgtt ttcaacgaag tagatctgtc ggtctgatga
    19921 tttggtacag ctgatacatg agctgtgatg gacgaaatcg gataagcaag gcttgttcca
    19981 tattggattt tcatacgctc cactgcatcc gtgttatcac ttgtccatga ctgcggcata
    20041 taataaataa acccggcatc aaaccgtcca cctccaccag aacagccttc ccataagatt
    20101 tccggataat cagttgtcaa atgttcaagt agttcataaa gcccgagcat ataacgatgc
    20161 gctacttctc cttgtctctg tgggtcaagg gtaatcgaat agacatctga aagcgaacgg
    20221 ttcatatccc atttgatgta gtcaagagga atcgtatcta aaatctttct catttgtttc
    20281 tcaatcgtct gccgaacatc taaccttgta aaatccagta catgttgtga tctggaagca
    20341 gaaggcattc tacctggctc ctgcataaga aaatcaggat gtgttcgata cagttcagaa
    20401 tcaaccgaga tcatttcagg ttcaaaccat aaaccaaatt tcaatccttt gctatgagca
    20461 taatctgcta tttcacgtaa tccatttgtc aacttgcctt catactcgaa ccagtctcct
    20521 aatgaagaat tgtccgcatc tctttttcca aaccagccat catctaatac gaacatttcg
    20581 atgcctagct cctttgcttc atcaatgatc gccttgattt tttcactatt gaagtcaaag
    20641 taagtggctt cccagttatt gaccagaatc ggccgttcag caaattgatg acgacctctt
    20701 gcgattcttt cacgtagcaa ctcatggtgg atctgactca ttttgttcaa tccctgactt
    20761 gagtaagaaa ggatcatttc aggtgtttga aaagactggc ctgcattcaa ttcccacgaa
    20821 aaacgatggc tgttgatccc accgacgact ctcaattgat tgatctgatc tttttcaagt
    20881 tcgaaagaat ggttgccgga ataaacaaat tgaagtccga tcgcttctcc agtattttct
    20941 gtcgtatgat gatgaactaa agcaataaag ttattcatat ggtgactgct cgttcctcga
    21001 cggctttcaa atacttttgt tccagataaa acagattgcc gtgaaatctg tctttcattg
    21061 acatgtgctc ctggcaaagc aatgacttca tcaaatcgcc tagtatgggc aaagtctagc
    21121 tggaaactag ccgctttttc tagtttgatt gtttctccgg tttcatttct tatctttaca
    21181 gatcgtgtga tcactgctct atcagtaaat atggtataga acaaatcgaa ataaatcgct
    21241 gtttctctat ccttaagtga aatcaccaac gtttgtgcct ctgacttgtc catcacataa
    21301 gtttgaggca agccttcgat ctctggcttc ccatctgtaa tataatggct gtcatatctc
    21361 agatccagta agtctgatcc attcttgcgc tgaatgatcg atgcgggcgt tcgaaagtcc
    21421 atgctgccat gagaagagta ttcttgcggt agtgtatctt tcgacagaga ccggtcagga
    21481 tttaagggaa catttcctga aaatccacga tctcttcggg gataattttt attgtctttg
    21541 tactgcttga ctggtttgcc aaaataaata tgcgccaata cattgctttc ttctatcttt
    21601 atcagataag aaagaaatgt atttcgtaaa tggaagactc ggctcttttc atcaaataag
    21661 atgaaatttt tatctgtttt tctatgcgta atatccatca tttcactccc aacaaaacgg
    21721 aaaccgttta ctttttttta taattcctgt atactgttta ctaaacaatt ttgtcaataa
    21781 aaatttttta tttttttgag gagtgtgtaa aaatggttgg aattagagat gtcgcacgaa
    21841 aggcaaatgt ctctcctgct acagtttccc gtgtgttgaa taacgatcag acgatccatg
    21901 tggctcagac tactcggcaa cggatagtag aggcagctgc tgcgttggat tatgacatca
    21961 gtactcgtaa atatatcaaa aagagaatgc cttcgattgg tgtgatctct acgatcggca
    22021 aagcgagcga agaacaggat atctattaca aacatttgcg agtagggatg gaagaagaag
    22081 ctcgacgtct gcatttaggg atgaatcgaa tctataatct atctgataat cctaaacaat
    22141 gggaagatct agatcagtta ggtgcggtga tcgttgttgg gactgttacg aaagaatcga
    22201 ttaccgcctt gttaaaacaa aacatgcaaa tagtcgtcgt ggataaccct gacatccagc
    22261 aagaggtcga tatggtgtat gcagatcttg aacggatgac taaaaccgta ctggaattgt
    22321 tcttagaaaa tggtcataaa agaatagcct atatcggggg atataaagtc gatatggatg
    22381 aattcggtgg gaagcagatt tctataaatg aaaagaggat gcgtgcctat cagcatttta
    22441 tgatcgatca tcacttagaa aaatatatgt cttatcatct aggtggctgg acagaaaaac
    22501 aaggtgaggt acttgcgact aatctattag aaaatgaatc ccctcttcct actgccattc
    22561 ttgtcgggag cgatccaatg gcaattggtg tctatcgggc attgaaacaa aaaaatatcc
    22621 aaattggaga agagattgtt gtagcaagct ttgataatat cgagacaatc gcaaacctct
    22681 cccctggctt gaccagtgtt caaatcaatg cgaaatcaat tgggcaagca gctgttcgaa
    22741 tggcattgga gcgaattgac ggtatacggg acgaaccaat cattttgact tatcctgcac
    22801 gattgattgt tagggaaagt tttatcccaa gaacaaaaag gtattgaaag agaggttgtg
    22861 aaaagccgct tcgtttcttc atactcaaaa cgaaacggct ttttcacaac ctcttactta
    22921 tacttattca caaaatccaa tgtctgctgg taacaatttt gcgattcttt tagcgtgtaa
    22981 tcaaattggt attcatgggt aatctctttt tttgtatctg tataaaacaa ggattgcacg
    23041 ggtaccttta attctgtcaa ccgattttct aatgcttgac cttgctcctg gaatgagtat
    23101 gcatttccgt cagttatata cgttggcgga aattccttag aaacatggtc agccacacta
    23161 gcttctttca attgctcact cgttttccaa tcttttgttc caatcaacga ccacgcaacg
    23221 gttttgacga aaaacttcat aaatcgatca tccgaatgct gctgtgctgt tagcttcaaa
    23281 tcaacaggtc cgcaataaga aatggttcct ttcagactat cttttggaag tagctgtttg
    23341 attttcattt gtttagcata cgcatcattc gtttgtatag ctgcatattg caatgcgatt
    23401 tgtgaacctg cactatctcc accaaaaaat aattgttcca tgtctagcaa tttgtcgtct
    23461 ttttcttcag ctaataaatc tttgatcagt tcattcactt gaagaacttg attagggtat
    23521 tctgaatcag gcgctaattc ataattcatc gcaaccactg cgatgttggc gtctgaaact
    23581 aattttgtcg caaattcttt gacacccgat ttatctccgc cgacgaatcc tccaccatgt
    23641 acccaaaata gaacaggaac aggccccttg ctgttttttg gatagtagat gtcataagta
    23701 ttctctttat acttggactc atacgtcaaa tctgtttttt tcgccacgtt tttttctgct
    23761 tgatcgaacg tttttttatc agtgatttga acagtatttg aaaacatatg gttgatcaag
    23821 aatgctccag gtctggggga aagctggaag gcaagaaaga ttgcaaacgc gatcccaaga
    23881 atgattccga agaaccatcc gataaattta agaattttct ttttcataag gctccctcag
    23941 tagttagtat taagcattct gattatacca tagtgttatt tcctatcaag ctctcctaga
    24001 tgatttaatg attttcccca aacttcttct gcaatgcttt tgacaaaaga aattttttcc
    24061 attgttcttc aaacaaaaca ttttcttctt ctgttgaagc aaatccacat tgaggactta
    24121 agcacaaacg atccaaaggc agatatcgtg tcgcttcgta gatccggtca atcacttgct
    24181 ggtgctcttc catttcgcca tttttcgtta cagtcaaaat ctaagacaac taatttatct
    24241 tcagagactt tctctaaaag aacaaaactc ccagcacgtt cactatcgta ttctaaataa
    24301 taggcacgga cattttcttg agtaaaataa tgggtctgct acgtcgtcat cccccctgat
    24361 gccgccaaaa tcgaataata atttccgcgt caaacatgta tatttagtgt tgaatcattt
    24421 gagaatatga aaataaaaat ctttttgatc aatcagttaa tcaaattgaa ataacgaaaa
    24481 atctgagaca gaatgacgtt cattctgacc cagatttgat tttgcacaac tcaacagtcc
    24541 ttattggcaa atcgccaaaa taatgtagac taacta
//
LOCUS       DACDXO010000038        24221 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E23605_2017
            SAMN12639989-rid9881463.denovo.038, whole genome shotgun sequence.
ACCESSION   DACDXO010000038 DACDXO010000000
VERSION     DACDXO010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639989
            Sequence Read Archive: SRR10023389
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741914.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:42:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,972
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 33 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 17 of 73
            Pseudo Genes (multiple problems)  :: 10 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24221
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639989-rid9881463.denovo.038"
                     /isolate="E23605_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(107..631)
                     /locus_tag="IWM35_001709"
     CDS             complement(107..631)
                     /locus_tag="IWM35_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9919559.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(624..1097)
                     /gene="tsaE"
                     /locus_tag="IWM35_001710"
     CDS             complement(624..1097)
                     /gene="tsaE"
                     /locus_tag="IWM35_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HAP9919560.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            complement(1220..2200)
                     /gene="pta"
                     /locus_tag="IWM35_001711"
     CDS             complement(1220..2200)
                     /gene="pta"
                     /locus_tag="IWM35_001711"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HAP9919561.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            complement(2353..3033)
                     /locus_tag="IWM35_001712"
     CDS             complement(2353..3033)
                     /locus_tag="IWM35_001712"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HAP9919562.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            3159..4028
                     /locus_tag="IWM35_001713"
     CDS             3159..4028
                     /locus_tag="IWM35_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP9919563.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            4054..4569
                     /locus_tag="IWM35_001714"
     CDS             4054..4569
                     /locus_tag="IWM35_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9919564.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(4616..5179)
                     /locus_tag="IWM35_001715"
     CDS             complement(4616..5179)
                     /locus_tag="IWM35_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9919565.1"
                     /translation="MIRFARKEDAPEIAPLILVILKDMELPFLKQTGEQKTLEILEEA
                     ITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKYQLDASTRLFI
                     DKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLSVDEKNPKAKK
                     LYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(5234..5413)
                     /locus_tag="IWM35_001716"
     CDS             complement(5234..5413)
                     /locus_tag="IWM35_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9919566.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            complement(5490..5999)
                     /locus_tag="IWM35_001717"
     CDS             complement(5490..5999)
                     /locus_tag="IWM35_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9919567.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            complement(6095..6475)
                     /locus_tag="IWM35_001718"
     CDS             complement(6095..6475)
                     /locus_tag="IWM35_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAP9919568.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
     gene            6805..7554
                     /locus_tag="IWM35_001719"
     CDS             6805..7554
                     /locus_tag="IWM35_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter"
                     /protein_id="HAP9919569.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
     gene            complement(7738..9015)
                     /locus_tag="IWM35_001720"
     CDS             complement(7738..9015)
                     /locus_tag="IWM35_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9919570.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
     gene            complement(9094..9738)
                     /gene="pcp"
                     /locus_tag="IWM35_001721"
     CDS             complement(9094..9738)
                     /gene="pcp"
                     /locus_tag="IWM35_001721"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HAP9919571.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            complement(9937..10881)
                     /locus_tag="IWM35_001722"
     CDS             complement(9937..10881)
                     /locus_tag="IWM35_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HAP9919572.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            complement(10883..11569)
                     /locus_tag="IWM35_001723"
     CDS             complement(10883..11569)
                     /locus_tag="IWM35_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HAP9919573.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
     gene            12043..12363
                     /locus_tag="IWM35_001724"
     CDS             12043..12363
                     /locus_tag="IWM35_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HAP9919574.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
     gene            12517..13422
                     /locus_tag="IWM35_001725"
     CDS             12517..13422
                     /locus_tag="IWM35_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP9919575.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            13419..14192
                     /locus_tag="IWM35_001726"
     CDS             13419..14192
                     /locus_tag="IWM35_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP9919576.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(14373..15797)
                     /locus_tag="IWM35_001727"
     CDS             complement(14373..15797)
                     /locus_tag="IWM35_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP9919577.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            16008..16454
                     /gene="nrdI"
                     /locus_tag="IWM35_001728"
     CDS             16008..16454
                     /gene="nrdI"
                     /locus_tag="IWM35_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HAP9919578.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            complement(16519..18501)
                     /locus_tag="IWM35_001729"
     CDS             complement(16519..18501)
                     /locus_tag="IWM35_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9919579.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(18515..19411)
                     /gene="murQ"
                     /locus_tag="IWM35_001730"
     CDS             complement(18515..19411)
                     /gene="murQ"
                     /locus_tag="IWM35_001730"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HAP9919580.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            complement(19414..20475)
                     /locus_tag="IWM35_001731"
     CDS             complement(19414..20475)
                     /locus_tag="IWM35_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAP9919581.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            complement(20686..21213)
                     /locus_tag="IWM35_001732"
     CDS             complement(20686..21213)
                     /locus_tag="IWM35_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAP9919582.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            complement(21280..22782)
                     /locus_tag="IWM35_001733"
     CDS             complement(21280..22782)
                     /locus_tag="IWM35_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HAP9919583.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            complement(23040..24161)
                     /locus_tag="IWM35_001734"
     CDS             complement(23040..24161)
                     /locus_tag="IWM35_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAP9919584.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
BASE COUNT         6919 a         5167 c         4263 g         7872 t
ORIGIN      
        1 tttattcaaa aaaaatggac tgcgacacga cttttgtcac agtcccttcg tgtgtttttc
       61 tgccttctca aaaggatgac agtttctcac tcttttccta tttcttctat tcaatcaaat
      121 aactcatttt atatacatct aaaaattcac ctagatctgt tcttgcccct ctaggcatga
      181 ccgcttcgat ttggaagccc atttttcggt aaaggtgtac ggctctttca ttacgaactt
      241 gaacgtcaag ctccaatcga aaaagtacac ccatttcctt cgcccaactg atgatttctt
      301 ctagcataag agtgcctagt cccattcccc aatattcctg cagaatactg ataccgactt
      361 ctcccacgtg agacaaacga aattgactgt ctgccttgac agatgctgtt ccaataatcg
      421 tcccttcata gattgcaagc agcaataaat tattattgct ttcgtatata taatccaact
      481 cttcttctaa ggtttctggt ggtaacagga gttctgcttc atccaaaact aaaaattctg
      541 tctccttatt aacttgtccc atcatcaaaa gaatgtcttt tgcatctcct ggcactgctt
      601 cccgcaagat gatcccaact tcttcactca tgattttcag cccatttttc tagaattcgc
      661 tgtttgaagg tttcactttg ctccccgtat gagattagtt tcacataccg cttttctaaa
      721 tctgtatcac ttttttctaa aatcagttcc aaataatcct ccggcagtgc ttcttctaat
      781 agatttcccc attcaacaac ggaaagccca tcaccttcaa aatattcatc taaccctaaa
      841 tctgctccgc tagctgctac ccgataaata tccatatgat acaacggaat ccgaccttga
      901 ctatattctc taataatcgt gtatgtcgga cttttgatca tttgcgttat ccctaatcct
      961 tgggcaattc ctttcgtcaa cgttgtttta cccgcaccta gatcaccagt caatatcaga
     1021 ttgtcacctg gcattgcgga atgaccaatt gcctctgcga attgttctgt cattgttaat
     1081 cctgatagtt cgatcatttt tcatcttctc ccgctcttct ttctttttgc cagtatatcg
     1141 cgtaatgaag tttttttaaa gaaaaaatga actagtcaag aacagcagct gctgtttttg
     1201 gctagttcgc gtgtatttgt taatcaatca atgtttgtgc tgctgtgata atcgataatt
     1261 tatatacatc ttcttcattt gctccacgag acaaatcaga aaccggtttg ttcaagcctt
     1321 gtagaattgg tccgattgct tcaaagttac cgaaacgctg agcaatttta tagccgatat
     1381 ttcctgactg caattctgga aatacaaaga ctgttgcttg accagctact tctgaatctg
     1441 gcgctttcag ttgtgcaact gaaggaacat aagaagcatc aaattgcaat tcgccgtcga
     1501 tcatgtattg tggcgctagt tttttagcga ttttagttgc ttcaactact ttgtctactt
     1561 ctggcgattt cgctgagcct tttgtcgaga agctcatcat tgctactttt ggttcgatat
     1621 cgaacaattc tgctgttttt gcagaatcta cggcgatttc agccaattct tgcgcagttg
     1681 gattcacgtt gatcgcacaa tccgaaaaga catatttttc ttgatctctt ccacgtacca
     1741 tgatcatcgc tccgcttgtc cgactgatgc caggctttgt tttgatgatc tgaagtgctg
     1801 ggcgaacagt gtcgccagtc gaatggatcg ctccagagac cattccgtct gcaatcccca
     1861 tatacgtaag catcgtacca aagtaattca catcttttaa aatcttttct gcttgttctt
     1921 tggatgcctt tccatttcta cgttcaacaa aagtatccac catttcatcc cacttatcgt
     1981 aattatcagg gctgatgatc gtaaagctag atgttttgat tcctctggca tgcgctgcat
     2041 ttcttatttc ttcttcgttc cctactaaaa tgggttccac aagttcttct gctttcaacc
     2101 gggcagctgc gcctaaaata cgagcatcag tagcttccgg gaaaacgatt ttgatcccac
     2161 ggcgaacaat tttaaatctt aaactatcaa acaattccac aagatgacct ccagacgttt
     2221 ttatatgtat tatcatacac tattccagaa aaaaagaaca gtttatttta tctgtatcag
     2281 tcaaaaatgc tggaaaaatt aagtttttca catacaaagc gaactgcact ataacaggaa
     2341 agcgctgttt tattacactg tttcaggcag ttgccaatca atcggttctt ctcctaacgc
     2401 aaggagcgca tcattcgttt tcgagaaggg tttggaacca aaaaagccac gacttgcaga
     2461 taacggactg ggatgtggag atgttatgat gatatggcga ctcttatcga tcattttgat
     2521 tttttcttgt gccggttttc cccacaagat aaacacgatt ggtttttccc gttcatttaa
     2581 tttttcaatg atcgtgtctg ttaatctttc ccaacctttc ccgcgatgag aatacgcttg
     2641 cccttcccga acggtaagaa cggtgttgag taaaaggact ccctgtttag cccatgacac
     2701 tagatttcca tgttgaacag gtgcgattcc aagatcatca taaagctctt tatagatatt
     2761 tcttaacgat ggcgggatct ttacgccagg ttgcacagaa aaagataagc catgagcttg
     2821 attttctcca tgataaggat cttgtcctaa aatcaccact ttaacttctt cataaggagt
     2881 caattccaat gcttcataga tatgatacat atctggatgg atcttttggg acgcatattc
     2941 tttttttaaa aattctctta gatgcagata ataatctttt gaaaattctg aggacaaaac
     3001 ttcttgccaa ctgttatgaa tgatttcttt caaaaactcc acctcctaat aaaaagaata
     3061 actcacattt tcttcaatca caagggacaa tcttcaccct cagcacttga ttaatatggt
     3121 aaactaggaa caagaagcag aaaggaacga taatcattat gatcaaatta attgcatcag
     3181 atatggacgg cacattatta gatgcccata tgagtgtttc tcctgaaaat gccgaggcga
     3241 tacgttttgc aaatgatgcc ggtgttgagt tcatggtcgc tactggtcga aactatcaag
     3301 aagctcgcgc agctttagat gaggtaggaa tcgattgtgc gatgatcact ttgaacgggg
     3361 cacaggtatt tgataaagaa ggaaattcat tgtttaccgt gtcgattcca aataatcaag
     3421 caatctctgt tttagatatt ctagatgcta acggtattta ctatgaagta gcaaccaacg
     3481 atggtcttta ttctgaaaat caagcgaaac gaattgaaag ttttgcttcc atggtggcta
     3541 cccatttacc tcaccttacg tataaaatgg cgattgccat ggcctcagct aagctggagt
     3601 tattgcacat cacatacgtg gacagtattc gtgctatttt ggaagaaaag aatctggaag
     3661 ttttaaaaat tatttgtttt catacagaag ggccagctgt attaggtcca gttggaaagc
     3721 agatttctct tttaggcgat ctagccgtta cttcctctgg tcaaaacaat atcgaagtga
     3781 accataaaaa cgctcaaaaa ggaattgctg ttgcccatgt cgctcacgag cgtgggattt
     3841 ctttagaaga agtcatgacg attggcgata acttcaatga tgtcagcatg ctgcaagtag
     3901 caggagtcag tttcgcaatg ggaaatgcag aaatcgaagt aaaagattat gccaaatata
     3961 caacggacac aaaccttgaa tctggcgtag gaaaagccat attgcgcgcg attgcagaag
     4021 acttgtagtt ttatctagaa aagagggata tagatgtcag aatttttcat tcaggaaaag
     4081 cagcttggac gtgcaactcg gacaattgtc aaaaacgaaa aaggacattc cctgtttttg
     4141 atggttggtc gctggggaac aaagggcgat gcattgtctc tttatgccat gaatggcgat
     4201 ctcgttgctc acatcaagca gataagcctg acattcggta cgcgttttga gctgtataaa
     4261 gagttcgaaa aggtgggaac catgcagaaa atattcaatt ggcccggtga cttttattat
     4321 attcgtagac ttcactggac agtccaaggt gatatctata atcatcaata tcaaatccat
     4381 catttcaatc agcaaatcat gaaaatggat aaagcaacac ttttttcagg agattactat
     4441 gtattggatg tctcaaatga tgaagatgct ccagtttgta tttgtattgc tgcggtttta
     4501 gattactggc tctaccgtaa agacaaaagt cgcgataagc ctgtttctta ccgacttaac
     4561 ttaagttaaa aagatgtacc tgaaatgcgg aaatacgcat ttcaggtaca tctttttaga
     4621 tattcttttg catatgatca taaagatgac cactgatcat ccgctgaccg acaactttga
     4681 agtcatggcg ttcgtataat tttttagcct ttggattttt ttcatccaca cttagaccga
     4741 tgacggagcg attagctttc ttcgccaatt taggtaatgc ttctaataat cgcgaaccga
     4801 tgccttgtcc tctaaaatct tctgatacac tgatagagtc caaataccat tcgttgggaa
     4861 acgtttcttt atcaatgaac aaacgtgtag acgcatctag ctgatatttc tctaagatag
     4921 gtaccagtgg ctgatcgatg attgattctt cttcatctgt ataaccgtaa gcaactcctg
     4981 caactttacc gtctatttct tctacgatac ctcgtgaata actgtaacga taatttggat
     5041 cagtgattgc ttcttctaaa atctctaaag tcttttgttc ccctgtctgc ttcaaaaatg
     5101 gcagttccat atcttttaga atcactaaga tcaatggggc gatttctgga gcatcttctt
     5161 ttctcgcaaa acgaatcata gtttcctctc cttttctcca ttctaacatg cagaaaaaga
     5221 aatcgcaaaa tcgttatttt aaaaaatctt caatcttttc agtaatgaaa taaccatgag
     5281 taacgattct tgccgcttca gttcggcaat aagggcagac gactggcatt tctttatcat
     5341 tatgttcgac tacattattg acttcttcgt atgcaaattt ttttccgcaa ttagggcatt
     5401 tcattttttc catgttatct caacttcctt tcatctttac tctcttagct tactcttata
     5461 ttgtaaaaaa gagaagcaat atccttcttc tatgcagata tccatacctg acggtcctgt
     5521 tctccatcta ttaatcggac tttataataa ggacgttcat ctttgactag aagtgtccac
     5581 tctttcactt ttgctttttt cccgtagtag ctgactgctt ccttcatcgc tgcttgcggt
     5641 tgtttgaact cttccgtttt ttctatatca aaaagatgtt ctgcggcctt ttccgtgtca
     5701 tttcgtacgg ttgttttccc agtggatgga ttgactgcta tgacttgtcc ttcacaaaaa
     5761 aactggtatt catatgttgc attcgtagga ttctccaaat ctttttcaac tgcaaaatga
     5821 atagcagata tgttatgttc ttttgtttcg ttttttacga ttgctgctgc ttttttcaat
     5881 gagatccgat attcctggtc actaatatca tccatgtgaa aagcggcatt gagcagtccc
     5941 gtcataccgg taatccctat tacgatgagg gcagcaacac caaacatcat ttttttcatt
     6001 cttcattctc ctgtttcttt tttttaaaga atatcaagaa atgaacgaat gaaaaatcga
     6061 atgaaacgaa aaaaaagtga caattttgtt atctttattc gtgcagttct aatggcaatc
     6121 cgtctggatc aaagaaaaac gtcatttttt ttcctgtaaa ggtatcttct ctaatcggtt
     6181 ctgattcgat ccctaaggac tgcaattctg caattgtttc ttcaatatcg tctactttga
     6241 aagcaagatg acgcaagcca caagcttctg gatagtttgg tcgttgtggc gcattagcaa
     6301 ttccaaaaat ctccaattca gcacttccta gtttcagatc taatttatag tcatctcgtt
     6361 ctttgcggta attttctctg attacttcaa atcctaattt attaacataa aaatcacgtg
     6421 atttctgata atctgaaaca atgatggcta tatgatggat cgttgtaagg ttcatatgag
     6481 cactcctatt cttcgtttaa gctcggatct atattaatct ccagttccat tgtaaatgtg
     6541 tcagattgaa aaaaatagct gtttttcctt tttataagaa aaaaggcaaa aaaaacagaa
     6601 agtaggtcct ccaagacact cttcctgttc ccaatataat aaatgaaagt aagaaattat
     6661 attttcaaga aaacaaacag aaaattatca taacacaaat tatttataat tgaaagacta
     6721 gttttttttc aagaaaagca tacaatagat tgtgttagta aatacagaac acactacatt
     6781 tagtgtctta aggagtcctc atacatgaag tcatattttc agtttttagc tcatcagcta
     6841 aaaggctggc cgcaacaaaa ttattatctt ttctttttca gtcttggctg ccaggtcatg
     6901 actcttgtca atagtcccat cacttggctg tccgttatta catttatcgg taccacgctt
     6961 ggtgtattgt gtattttatc catcaatgcg gcaaaatcag tcaacggtat tttaggtatt
     7021 ttgtctgcga tttgttttat tatcgttggg ttttcagcaa agaactattt aagtatcggt
     7081 gaacagattg cttatatgat cacattggat atccccgtat tgttaagtaa agaatggaat
     7141 cataatatgg cttcaaaaat ccgaaaattc actggaaaaa gctggtttat cgcaatcatc
     7201 agtactttcc ttgtctatct tgtttcagga ttcctgattg gtcgttatac agatgatcct
     7261 cggccgtggg tagatgccat tgctttttct attagcttga ctgctggtat catcagtttc
     7321 ttgcgttaca acaaccaata ttactggtgg ttagcttcag ggcttgctca aatggtcctt
     7381 tggtatattt catttacgca tggatctgca agtttggcga tgttcgtgaa cagctctgtt
     7441 tacttgctga atgatgtcct tgcatttacc gtttcacctt ggtataacca aaaagaacgt
     7501 gaacgtatgc aaaaaatgga agaagaatac tacaacgcac aacaacacgc ataaaaaaac
     7561 tgagcgtggg acaaaagtaa agaacacttt tgtctcacgc tctaaacacg tataaacggc
     7621 ggaatcagaa gcaactcctt cggaaataag ctgaaattca caaaaatttg aaaagcaatt
     7681 ttcgtgaatt tcttcttatt tcttggagtt aaacgcttct gtcccagcct ctatttttta
     7741 tagtaattcc ttcagatact ttcttgcctc atcattgaaa cgcagtgatt cttctaactt
     7801 ctgataatat tccaacgctt gtttctgagc ttcagtaaaa cgataatgtt ttcctttcca
     7861 tttgtcgatt gtctcttgca catttccaga ttggatatct ttcactacta gttgccattt
     7921 ttcttttgag actgtacggt ctttcttatc agcagattgt atagcttctc gttctttacg
     7981 caatactttt aaaaaaccat ttctgactat ccgaggaatg ctgtcgaaga acccttctgt
     8041 aaaaccatag cgacaatatt gcttccaaat aaatagatag atttttggtt ggatgaatcg
     8101 ttggtttttc gcaaaatatt ccatctgagc agtgacttct ggtgttcgtt gacgttttgt
     8161 ctttctcaac acggaaataa atgtttcaaa ataagcatta ggatgtactg gcacttcttc
     8221 atatacgatg gattgccaat ctttacgtaa aaatgcgcga tagttgatca agaattcttc
     8281 tacatccttt actaattctt ctgcaaagtc gtcaaatagt tcttttttta tctgtcgctt
     8341 ttttgactgc ccattgatgt gttcacttat tttcttgatt tcattttcca atgcttgata
     8401 atccgctatg taaatcggca tttctacttg gcgaaattct gcgagccgct gagctaattt
     8461 tatatccggt aaatatggct gtttcatccg acgattttgg tacaagacaa aacagtattt
     8521 ttgccatagt ccttcaggaa aatcgatatt cttctgctta agccataata attcgtctaa
     8581 ggccagatca acttttgtca ttccctgatg gatcgaagct gccactgtcg gagagacatg
     8641 caggtgatcg gcaaagtggt tgatgcctag ttttaggatc tttccggttt ttggcgattt
     8701 aacgatgtat tgcactttta attctctacc acactcacaa tacaattttt gctggcttcg
     8761 ttttgttcga aaaaacggat tgatcttgaa gtcagaaaac acccaatctg ttgcagctaa
     8821 ataatgttct tccataaaac gagagctgat tagatatcgg cggtgcttat cgactagttc
     8881 ccgctgtttt ttcgttaatt ttgcatagat ctcccaacgt tgactgcttg tgtattgttt
     8941 agaaattttg atgggatagc ttttgctgat ttcttttgga aaccattctt caaagacgga
     9001 ttgccattct tccacggctg tttctttcct ttctaattac aaaaagctgg ctactaacat
     9061 aacgttagca accagccagt gtttcttgct aaattagtga gttgttccgg catttccgga
     9121 aatatcttct ttattttcta ctgctgcttc aactgcataa gtcaaagaag aagcaatgtc
     9181 ttctaaagac atagaaggcg tacccactgg acgatcgatc acttgatcgg gtgagaatgg
     9241 cacgtggatg aacccacctc gaattgccgg gaattgtgtt tcgatcaaat gaagcaaacg
     9301 atacatgata tcattgcaga caaatgttcc tgctgtataa gagatgtacg ctggtagacc
     9361 atgatcttgt acattttttg tcattgcttt gattggcaat gaggtaaagt aagcggctgg
     9421 accgttttct tctaaagccg tatcgaatgg ctgatttcct tcattatcag ggattcttgc
     9481 ttctgctaga ttgattgcga cacgttcgaa cgagactgct gaccgcccac ctgcttgtcc
     9541 gacacagatc acgacatctg gttggtgctg ttggatggct tctttgacta cttgactgct
     9601 tttttcgaat acggtcggta cttccacttt gatgatttca gcaccgctga tttcatctgg
     9661 catttttttt actgcttcat aggctggatt cactttgtcc ccaccaaatg gatcaaaacc
     9721 tgttactagt actttcatct gttgttcctc cgtccgattg atttaacata tggttatctt
     9781 ttatcatacc attttccttg aacagcgtaa ataaaggaaa caatagttta aataacataa
     9841 cagatcaaac ttaatatatt cttataaaaa aatagtgttc cactacaatg ttagaagagg
     9901 ttgcgacaat tattctgtca caacccctct cttctattat ttaaatagaa tcatataagc
     9961 aatttggaaa actagcataa ataacgcaat aaacaactgg tttttgatca ctccgtattt
    10021 gtctttcatc tccagcatcg ctacaggcac gatattgaag ttagcagcca tcggtgtcag
    10081 cagggtccca caaaaaccgc aagtcaatgc aaccattcct attaacgctg gattcgctcc
    10141 atgactaaat acgaatggtg caccgatccc aaccgtcatc acggtgatgg ccgcaaatgc
    10201 attccccatg attaccgtaa atatcaccat gccaagagcg taaatgatga tgccgatgtt
    10261 cacatttcct tctggaacga tcgtacctac tgcattggaa atcactgtcc caactccagc
    10321 gcttgtaaat accgcaccta gagaagctaa aagcattggc aacatgctta acggccctac
    10381 tgtgcccagc atatctgctg catcatctaa aaatgtactt ggtcgattat ctcgagaaaa
    10441 gaacatcaag attagcatgg ctgcaaaaac accgacgccc acgccgacta aagcacctaa
    10501 attggtaaat agagcaaaca agatcgcaaa gacaccgata ctcaaagctg gaataaaaat
    10561 tttcattcct agtttgtctg acattttttc catatattct tttgatggaa gccggctttc
    10621 tcctttgctg actttcttag caattgctgg tatggtcatc gcaaagatca agccaccgtt
    10681 cacgattgcc ggaagccatc ttcctcctgc aataactacc cctaagaccg tccagaaaaa
    10741 ggctgaccca taacgatgag gattggtttt atctgttaaa tttcttactc cagtgtaaat
    10801 caatacgagt cccatcacga tataaaggat ctccagtaat ttatcgccta ataaaatatc
    10861 aggatttgta aaaaagtcca tcttattgcc cctttcctgg ataatatttc ttcgctaatt
    10921 tcttttcttt aagatagaag tagacacttg ccacaactaa agccgtgata gccactggaa
    10981 cttccactaa agcaagctgt cccaatgtta cgtcatatcc tagatctttc agtgttgatt
    11041 ggactagcaa cgcccctgct cctccgatga atagtacttg gccaaagaac cagcagacat
    11101 tttccatagc tgaagccata ccttttagtt cttcttcatg cttaggatgg acttctaacc
    11161 cttttgattc gatggtcccc aatgccatcg gtaaaatgat tggacgaaca aatccagcaa
    11221 ctccaccaaa actcacgtta aaagccgcaa agatcattcg aaagatccca taaatatcta
    11281 caatcatacc agcagatact ttcttgaatc gcttgatcaa ggaagccgct gattctttca
    11341 gaccatttct ttcaagcgtt cctgttgcca acataatgat gataaaaatc gccatcccgc
    11401 gattgtttac aaaacttgta cctaaaagtt ctaacattcc ttgaatgcca agaccgccag
    11461 tcaaagctgt aacaactaaa gcaaccatta cgatcaatat cgagtctaat ttaaatgcaa
    11521 acccgagaat aacaatcaag acgcctaaaa ggctaagata attttccata tattttcctc
    11581 cataaaatga caatattttt ttaattataa agcaaaacac ataaaaaata tagttgtttt
    11641 ctcacgaaat agacactatt tttaaatatt tttcgttatt tttttatcac aaaaattatc
    11701 ttacttattg taaggtaaag ctttttttaa tttattgccc gataatatat agaaataagg
    11761 tctagttact gtttttttta aatgattaat aaataacaac ttctttctat gaaattgcaa
    11821 agcattcatc ttcttttccg ctaacgatat atccgtcagt gcttggtaca ctttactaaa
    11881 aagtatgaga ttgccgatat ctggataggt gtgctatact acagaaaaag atatacaatc
    11941 tgccaccggg taggataaag aggatgatca ttccattagg tctttgaagg gatgattttc
    12001 ctctttttgt gtattttttt atagttaagg aggaatgtaa gtatggcatg gatggaatta
    12061 atcatagcag gaattttgga agtattctgg tcaacgatga tgaaatggag tgatggtttt
    12121 tctaaaatca attattcttt ttacacagtg attggcatga ttgctagttt ttactttttg
    12181 tcaaaggcaa tcaaaagttt gccgatgagt ctctcttatc ctatctggac agggattggc
    12241 gcagttggtt cggtcataat tggtgtggtc ttgtttcatg ataagctgaa tctttccact
    12301 tggttctttg tggggatgct actcatcagt atcattggca tcaagattac tagcggccac
    12361 tagacacttg gaataagtac tgatcagaaa aaataagcct ttttatttaa tattcaattt
    12421 ttttatgaaa aaataagaaa taagtccaat gacctagcga cttctccact catcaggtgt
    12481 atgcttgtac tatctataga aaaggagtga atatttttga aaagtctttc ggtccaagaa
    12541 ttatccaaga actttggtca caaacaagcc ctcagcgatg tcagttttag cttatcccct
    12601 ggcaaaatcg ttggattagt aggtcctaat ggcgctggaa aaacaacgat catgaaagct
    12661 attttaggac tgatccatta ctcagatgga acaatctgta ttggtgaaaa tgagatttct
    12721 ccctctagtc ataaagggtt ggaacaagta ggagcattga tcgaataccc gggtatctat
    12781 ccattcttaa caggctatga tcatctgaag ttattctctg aaaccaacga tgtatctgct
    12841 atcgacacta tcgtgaatca gctcaagatg gaaaagtaca ttcacaaaaa agcgaaatcc
    12901 tactcgttgg gaatgaaaca aaaattggga attgctcttg ccttactgaa ccatccgtct
    12961 atcgttattt tagatgagcc gatgaacggt ttggatccac aagcaacaag agatgtgcga
    13021 aaaatcatta ccgacttggc tactcagggc acctctttct tgatctccag tcacttactc
    13081 agcgagcttg aaaaaatggt cgatgagctg atcctgatca atcaaggaaa gatcatcaaa
    13141 caatgtacaa tggctgaatt gaaccaaaca gcgcaagatt tcatcgttat ctctacaaca
    13201 aatgatctag ctgctcgaac gatcttgaca gaagctggat atactttgga agatcctgat
    13261 aaaatcaagc ttttaaaaga aaacgaagaa ttattaggaa ccgtgatcca gctattgtct
    13321 gcacaaaata tccatgtgac agacgtccag catctgacga atgatttgga atcttctcta
    13381 ttggaaattc tggattcagt aaagggggaa gaagaaaaat gacattgctt gtaaaacaag
    13441 aattatttaa attgatcaag aaaaagtcaa ctgctgtttt atctgttcta ttagttgttt
    13501 tgctcatagg aacagcactt ctagccaaaa aatacacgac gatcattgat ccagttgaaa
    13561 tgactgctca gctttttagt gccacatctt ggattgtctt tatcatgatt gcagctgcaa
    13621 gcacgatcat ttcgatggaa gcacaatatg gtacattgaa aaatctcctt tatcgaaagt
    13681 attctcgagg agaaatatta gtaagcaaat ggatcacatt agttatttat tctgtttatt
    13741 tatacctttt agctattatt gtcactgtgt tgatgaaact gattcttttc ccttcgattt
    13801 ccttcacgga gaaggtttct actggtcaaa cactgatcca aagcttgttc acttatactt
    13861 tgggtagtta tatcggttta tggctgattt taagtttagt gttgatgatt gcttgcttta
    13921 tcaatagttc cggcgcatcc atctcagcag gaatcgtctt ttattttgct tcgtcaatca
    13981 tatctggtat ccttattgca ctcatccaaa aatgggagtg gatcaaatgg aatccgatca
    14041 gtatgttgaa tctgcaaaac caaattggaa acgaagagat catgaagcaa ttgactcatc
    14101 tttctactaa tcaaatgcta ttcggtaatt tagcttacat cgttttgttc ttagcattag
    14161 gatatctcgt atttaagcga aaaaatgtat gatgactcat caaaaaagag gctgtgaaag
    14221 agacgttcag ccttattgcc gattggctgt ctcttaaaca ccgtttattc tgaaaaagga
    14281 gctgtgacaa catttgtgtc acagctcctt tcctagtatg tttttgaata aacgacaggc
    14341 tcacaacctc tctttatact tcttttatac ttctatacgt gaagcatccc agtggcaaat
    14401 aaaaccattg caatcactcc gaccatcgtc acacctataa gtaaaatctt atctaagtgg
    14461 atatgttgta caggctcgtt ttgctcctta cgtgctttga ggtaaaaata aatgcctggc
    14521 aagaaaagaa caagaacaag cattacttca ttaaaggcta cgactagcat agcccatact
    14581 tgaaaggcac ttgctaaaag ccctactaaa aattggaccc attctttccg ctgataagaa
    14641 agtttcattt ggtacaatcc taagaaaagc cacgtgaaga aaatagctgc tgtaccaagt
    14701 gtgtaagcaa agttataagc actgtcagtg aataataatg aaagtaaaaa gacattcgta
    14761 cataacgtcg tcaatacgat agcataagtt ggtgccccat ttttgttgag ttttccccat
    14821 gctttaggca aaagtccgtc ttcagccatg tttcccattg tttcaatcgg taagatcgtc
    14881 catgagagcc aaacaccaac taaagaaatg atcaaaccgc cgttgatcac gactgctccc
    14941 catttaccaa ccatcatttc aaacacatag cccatagcag gctgcgcctt gatcgtttgc
    15001 agctgattca tcgtcattgt cccatatgga agcatggaaa ccaagacgta aataataatc
    15061 aagccgaata tccctagaat cgttgctttc ccaacatctg attgtttttt tgctcgtgaa
    15121 cctacgacag aggctccttc aataccaaca aatacccaaa gcatcaccat gatgcaactc
    15181 aatacttgac tggaaaacgg gatcggctgt ccattcgcat tcaatataat gtcgctagaa
    15241 aagttatcaa tgaattgttg gatcgaaaac gtgctgatag ctactacaat aaataaaaat
    15301 aatggcacta atttacagaa tgtgacgata gcattgacga aactagcatt ttcgactccc
    15361 cgattcacta gccatgcata tccccataaa aagactgtac tcatcacgat cgatgccaga
    15421 ttttgcccac cttcaaatac tgggaagaaa tatcctaatg cgctcatcaa caatgttgca
    15481 aaagctacgt tgcctaacca gcatgtgagc cagtaacccc aaccgctgat aaaaccagcg
    15541 aaacggccaa aaccttcttc tgcgtaacta aagatcccgc cttttaaatc tggacgtttt
    15601 ttattgatat tattgatcga caaggagagc atcaacattg ctgttcctac gagtacccaa
    15661 ctgaaaatag ctgggccgcc agccgatccg gagatgtccg tcataatacc aaatacgcca
    15721 cctccaatcg ctgaaccaat tactaaggca gcaagtggaa ttaaaccaat tccttttttc
    15781 tgttgctctg tatccatgta agcatcctcc aaaataaaac cgcttttatc aaaaagtata
    15841 acaaaccttt tttattttta cttaattaat aaaaacttta atagaaatat aattctaccg
    15901 gcttttttct cattatacaa gcaaagcgcc tagtcccttt ccaaattaca ccaccaaaaa
    15961 acttaaaaaa gatgctatac taaaaggaaa gaattggagg atgatccatg aacattttat
    16021 atatatcgat ttcaggaaat acgcgtgcct ttgccaaaca tctagcagaa tatgctgaaa
    16081 aaatgcatgc agaagatcct gtcaatcctg aagttacttt aaaagaaatc catgaaaata
    16141 gtgattttac gaaagaaaca gaaccatttt ttactttcgt tcctacttat ttagacggcg
    16201 gaaatggttt agataacggt gatacagaaa tcttgaccga aacgatgcgt gaatatctgg
    16261 aacatgaaga caatcataaa ctttgtcttg gtgtagtcgg tagcggaaat aaaaacttca
    16321 ataatcagta ttgtctaaca gccaaacaat atgcacaaac atttggcttt cctttcttgg
    16381 cagattatga attacgggga acaccatctg atgtgacacg aatttatcag attttagcag
    16441 aaaacaataa ataataaaaa agaagaagct gaaaaataga tttcagtttc ttctttcgtt
    16501 tctattgttt ctatcatatt aagctttgac atccatcaca ggtgcttttg cttttgcttt
    16561 acctgttttt tccaagacaa agttttttac acgatccata ttggttacca agacgatcat
    16621 ggatgtttct ttgcctgctg ctttgatcgc ttctaagtca gcttctgcta caagcgtatc
    16681 aggtgtcact tgctgccctt ctgttacttt cacatcaaat ggcgtaccgc ctaagtcaac
    16741 tgtgttgatt cccatatgaa gcaagatttc taaaccattt ggcgttgtga tcccaatagc
    16801 gtgtttcgtt gggaagacag agctgactac cccagtaact ggagaataga tatgtccgtt
    16861 ttctggttct acaaagtagc cttcacccat catcttttgt gcaaaaactg gatcacttgc
    16921 tttatctaaa gaaacaactg ttccgtcagc aactgatgag aggatagtca cactttcagc
    16981 tgaagtttga ggtactactt tttcaggcac ttcatttact gtggctgttt cagtcgctaa
    17041 ttcttctttt tctaaacggt ctttaggaat accgaagaag aatgtagcaa cgaagccacc
    17101 agcataagct gccaataatc caaggatgta gcctaaccat ttaccgtcag agatcaatgg
    17161 gatcaaagcg gctcctgaag gtccaatagc gatagccccg acatttccga tcattccgat
    17221 cactgccccg ccgatacctc caccgataca agcagtaata aatgggcgtc ctagaggtaa
    17281 tgttactccg taaatcaatg gttcgccgat tcctaggatc ccgactggta aagctccttt
    17341 gatcatttct gttaattctt tatctttttt gcagcgaatc cataatgcca atgctgcacc
    17401 cacttgtcct gcaccagcca tcgcaagaat tggaagcaat agcgtacgac cagtttcatc
    17461 aatcatttgc acatggattg gagtcaaaat ttgatgcagt ccgaacataa ccatcggcaa
    17521 gaataatgtg cctagaacga agccggcaaa tgctccacca acgccaagta cccagttgat
    17581 cccaccgacc atgccatcag aaatgaaacc tgctaaaggc atgatgaaga aaatttcaat
    17641 cagaccgatg gcaatcaatg aaagagttgg tgtaacgatg atatctaccg catcaggtac
    17701 tactttatgc agttttttct caacgattga caatagccaa acagcgaaga tgacacctag
    17761 gatcccacct tgtccagctg caagagcttc accagtaaat aagtttttca gtggtgcttc
    17821 cggattcatc cctgtcagca tcacaacggc cccgataact ccaccaagag ttggtgttgc
    17881 accaaaaact tgtgcagcat tgatacctgt atagataact aggtagctaa acatcccgtt
    17941 tttcaagatg ttcatcacat cgatatattg ttgccatgtt gctgcatcaa gtgttccggc
    18001 tgtgactaag tttgctaaaa ctgcggcaat ccctgccacg atcccagtac cgacaaatgc
    18061 aggaatcatt ggcacaaaga tattagaaat gtcttttaat accgatttga ttttagatgg
    18121 tttttgtttc gatttttgtg ccgctttcat ttcttgtgcc cgttcgttta cacggtcttt
    18181 tccagatgct gatgaatttg ctgtagctgt acttacagaa gaaagttttt ctcctaattt
    18241 cacgcctgcc aaatcaacca tttgctgtgc tactttgttc actttcccag gtccgatgat
    18301 gacttgaagc tgttcatctt ctactacacc aagcactcct ggaatcgctt ttaattcaga
    18361 gacatttaca agatcatcat tgatgatctt catacgcaca cgagtcatgc agtggacaat
    18421 gctttctaca ttgcccatgc cgcctgcccc tttataaatt tgttttgcca gttcttccaa
    18481 agtcagttcg ttttttgcca tggtcttttc cccctcataa ggtccctttt acaaatccgt
    18541 ttgcttcagc taattttgct tgagcttcct ctttatcact atttgtcaaa atcatgacga
    18601 tagccagctt aacattttga tctgcttctt caaagtactt ttcagctgtt tcgtaatcag
    18661 cttctgtcgc ttgcatgatg attcgttttg agcgttcgat tagtttttca tttgttggtt
    18721 tgacatcgac catcaagtta ttgaatactt tacctatacc aatcatactg atagtagaaa
    18781 tcatattcaa gataagtttt tgtgctgttc ctgacttcaa tcgagtagag cctgtcaaaa
    18841 attctggacc tgcatcgact tcgattggga aatccgcgtg tttactgatt tcggcttgtt
    18901 tattgcaaga aatcgttcct gtgacagcac cgatcgattt tgcgtaatct aatccaccga
    18961 tcacatatgg tgtacgaccg ctagctgcga taccaataac tatatcattt tttgtcaaat
    19021 tcagcttgat tagatcttct tgccctagct cataagaatc ttcggcccct tcgacagcta
    19081 cagtcattgc ttcttctcca ccagcaatta gacctatgac catttccggt tctacaccga
    19141 atgtcggtac gcattcagca gcatctaaca cacctagtct tccacttgtt ccggctccca
    19201 tataaatcag acgtccacct tttttaaagg actcaatcgt gttagcaatc actggttcga
    19261 tacttgtcag ttctttttca accgcagcgg ccactttctg atcttctttg ttcattagtt
    19321 gaacggcttg agaaatactc atctcatcta atccaaaagt ttcttggttt ctgcgttccg
    19381 ttgttaggtt ctctaaattg attttgctca tttttatctt ccttcttttc ttataaattg
    19441 atagttcata ccagctttga tttggttgat caatggtagc tcttctttga taactttagc
    19501 tactacattc actttttcat ccgctggcaa atcatatttg gttaattgga tctcacccat
    19561 atagcgcaaa tacttttcgt tatctaaagt aatagaccct ttcatacgcg tagctgtatt
    19621 tcttgctggg ataggcggaa tctctcgaaa acgtgcatct acgcttcgta tgacatctct
    19681 tgcatcgtct tgccgattcg tatgcttccc taatatatat tcgtaaaatt cttcatctac
    19741 aggttccgta tggagcaaga ctgtctgttc tttttggaaa gaagcaaatt gctcctgaac
    19801 gttttctgac agacctgcat ctccgatgta aacaatatcc gtgttacttg ctaatagatc
    19861 tagagcgcct gcaagaggat ggatccctcg atgttcttcc aaagttggca atccttgata
    19921 tagtgggcct ctcaactctg tatctcctgg tacaaaacct tggacaagga gaccttgaat
    19981 tttgagccat tgattttttt tctgatacca ttctttgtcc agccctgttt cgggacgtgg
    20041 ataataatta tgccaagctt caatctgtga gaaatcagct tctgcttctt tcaattcatc
    20101 gatatcttta ggcgtgatcg tgctagcatt caaactgatt ttcaattgat gtgaccagtc
    20161 ggcaatctgc tgattggaaa tatgataatc cattcttaaa cctgttacac caattgcttt
    20221 caaaggcgtt agatcttcta atgaaaaacc cgctcgttcc aatgcctctc ctgaaatatc
    20281 gaccatcaat tctagtttat tctttttcgc ttcgcttcct agttcgatca atcgcttttg
    20341 atatgccgaa acatcatctt ctggtatatg catggaagtg aaaataccga caaagccatt
    20401 ggatgccatt tggcggatat actgtttctt ctcttctgac aaagaatcgt tcataaatat
    20461 tgaaaagcca aacatattat ccctcttttt ctgtatccgt cttcatttct atgactagag
    20521 tatatatcta aagaaacaaa atttcaatac ttttattaaa ataattaaat ttcatttcta
    20581 aaggttgagt ttatattatc aaaagaattg tcataaccta aaaaaggcac tgctaagcaa
    20641 aagcagtgcc ttgaaatatt ttatttaaat aacacaaaaa cataattact cataagaaac
    20701 caattctgta ttatctacca tgaatgtaaa gaacccctca ttaggcatcg gatgaaaata
    20761 agcattgatc actcttatca aggcattatg gcaaactaac agatagacat tttcttcgtc
    20821 ttcgatgcat tctttcaata aaggaacgat ccgtgcatac acatctaaga ctgactcccc
    20881 atttgggaat cgtacagcga acgctaaacg tgctttttga aagttctcat ctttgcttgg
    20941 cagtccatca taatccccaa aatccatttc taccaatcgt tcatcaacgg tcagcggcaa
    21001 gctgagacga tcagcaactg cttgtgctgt atctcttgct cgttgaagag gggaatggat
    21061 gattttcgtg attggaacag gcaaatctac taccttttct gctagtttct gtgcctgcat
    21121 ctgtcctttt tcagtcagcg gcacatctgc ccgtccgcaa actaatcctt gtgcgttcca
    21181 agttgtttct ccgtgtctcg ttacataaag catgtgattc tctccttcgt tggcaaagtt
    21241 ccccttaacc ataccttgtt gttcttgaga ttacaagggt tagattctct caagagaaat
    21301 aatctcagta gatactttat ccaggaaggt ttgatcatgt tccactagta acatggcagg
    21361 tttcacgttc aagatcagtt gttctaattg ttcttgattg aagacatcca aataattcaa
    21421 tggttcatcc catgtatata gttcagctgg ctgtgataaa gatttagcca attccacttt
    21481 tttccgttgg cccatactca tctgctcgat cttgttatga aaaacatctc tttccatccc
    21541 aagctttcgg aggttgttca aaaatgcttg gtagtctact tggtttttct ctgcaaattc
    21601 cgccaacgtt cctcgattgt cttcataatt ttggcttgca taactaatgc tcagatgttt
    21661 tggctggtat ttttctccta taaccttgcc gttgaatgcc cccagaagat aatggataat
    21721 ggatgacttt cctgcaccgt taggacctga aatcgccacc cgctgatgag gctcgattgt
    21781 aaaatgaatt ggctcgaata acagattttc ataccctaat tgaagatctt ctacgcttaa
    21841 aagtcgctta tggtgagacg cttggctatt catcgtcaaa gaatcgatat attcgatatc
    21901 ttttaatagt ttttctttct cgctgatctg ggtctccatc cgattcacga ttgctttgga
    21961 tcgtttcatc gtccgtgcag catcagcacc cactgctcct ttattcactc gtctagattc
    22021 agtatcgatg aatccgactt gtttctttgt tttatctcct tctcgggaac gagaccattc
    22081 ggctttttca gctgctgttt tctttagcct actgacttct tttttcaatt tttcattttg
    22141 agccatttca aattcatcac gaagtttttt ctgttcttca tagatagaga aattcccttg
    22201 ataaagttcc agttgacttt tttcgattgc taaaacatgg tccactactt catcgataaa
    22261 tccccggtca tggctgacca cgatgaagcc ttgttttttc tttttcaaat aagccgctac
    22321 ttgttttcta ccagagatat ccaaatgatt cgttggttca tcgattaacg ggaaatgagt
    22381 gtcatccaca aataaaagtg ccagtaggac ttttgtcttc tctccccccg atagtgtgct
    22441 gaattctctc cataagattt caggatctgt ttgcatcaat tggagctctc tttcgatttc
    22501 ccatatctca aaatccgtaa tatcatttaa cacgtaatag gttaaacgtt ctttatcttt
    22561 tgtctgttgc gggaaatagg caaattcttg ctgatggatt acttgccctt gataaggtag
    22621 tttgttttgt agaatattca gtaaagttgt ctttcctcga ccgtttcgtc cgataagtcc
    22681 tagtttccat tgtgtgtcaa aatttagatt tgcttgttca aataataatg tgccttggct
    22741 gtcgtagccg aatgtcagat tttttatttc gattttagac attttccatc acctcgtaaa
    22801 aaaagaccag tcctattttc agaactggct aaagaggatg caagggttgg atacacacaa
    22861 aaagacccat acatcgaaaa aaccaagcca atcccgaaaa tctgcacact gcttccttgt
    22921 ctccacaagg tacagtctga atgcagcttt catcaatcga cttaattttt caatgcacgg
    22981 atccctcact ttgtcctata tttgaaatta cgatagcata aagaaaatga ttttgtcaac
    23041 tacttcggga ggatggtgaa aaatctgcta agtgtctgtc ctaagatttt tgttttttct
    23101 aatgtttgcc tgtgttccat tgcttcttct aaacctattg gacccaaatg aatacttaag
    23161 acaattggga gaaaatcaga aagtccttct agctcttggt ctctacttcc gcaaatagcg
    23221 attattggtt ttcgatattt ttttgcaagc actgctacac cgtatggaac ttttccgtgg
    23281 atcgtttgct gatcgatccg cccttctcct gtaatgatga catctgcttg ttcgatcttt
    23341 ttttccaatc cagtgatttg gctgactaaa tcgaaaccag aaaccatctt cgctcccaac
    23401 aaaaaaaggg ctccaccaat accgcctgct gctccacttc ctggaatatc atctaaatcc
    23461 acctcccaga aatcttttat ttgctgagca atctttcctg cttgtttatc taagaaagct
    23521 agcgtcgtat ctattcctcc tttttgtctt ccaaattgcc aagcagctcc ttgttgacct
    23581 gtatatggat tggtaacatc tgcagcgata atcaatttta ctttagaaac aagaacgttt
    23641 cttcgatcaa aatgaagttt tttagtagat aataaaggat ttccttctgt gtacccttct
    23701 aaagaagcac ccaaggattg gagtaatcct agaccaccat cgctacaccc gcttcctcct
    23761 aaagtgacaa taatttgttc tacttcttga agcagtgcat ctttaataag ttctcctaag
    23821 gcataactgg aagcaagtcg gatagtttga tccgttggtt tatcaagtag atctaacccc
    23881 aatgcttgtg cagattcgat gactgcggtg ttttttcctt gccaagttgt caataaatag
    23941 ctgatttttc gctgacggaa taagagatcc atcccatcaa tggtacgcca tgttcccctg
    24001 agcgcgtaat agattgcttc tgcagtattt tctccgccat ctgcaatcgg cacattgatt
    24061 tttcttcctt tacgatcttc ccagccattt agaactgcct gatttagttc aaatgaagaa
    24121 gcacttcctt taaatgaatc aattgccgcc aaaatatcca tctgtatccc tcactttctt
    24181 cttaagtaag acactttttt tcattttttt caaacaaaac a
//
LOCUS       DACDXO010000039        23761 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E23605_2017
            SAMN12639989-rid9881463.denovo.039, whole genome shotgun sequence.
ACCESSION   DACDXO010000039 DACDXO010000000
VERSION     DACDXO010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639989
            Sequence Read Archive: SRR10023389
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23761)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23761)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741914.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:42:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,972
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 33 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 17 of 73
            Pseudo Genes (multiple problems)  :: 10 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23761
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639989-rid9881463.denovo.039"
                     /isolate="E23605_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..616)
                     /locus_tag="IWM35_001735"
     CDS             complement(<1..616)
                     /locus_tag="IWM35_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP9919585.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGKLDDSS
                     SYCEHCRSVRIIRNGSYTTRTQMLKVKEKLTILELKRTRFLCHDCGRTFSAKTDLVDE
                     HHQLTKELKQVILMELYENQSRKLIAKKYFVSDGTVTRILREATKHYQPRMNFLPTVL
                     CMDEFKSMNSVSGSMSFICVDGTTNQLFTILEDRRLYKLTQYFLR"
     gene            889..3360
                     /locus_tag="IWM35_001736"
     CDS             889..3360
                     /locus_tag="IWM35_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAP9919586.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGYQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKMIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLIKRNNTVAMTGDGVNDIL
                     AMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLVK
                     NIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNVM
                     EKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKFR
                     SQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLFE
                     RKNEKEQKPFSRVAKKFLSIFKREN"
     gene            complement(3463..5199)
                     /locus_tag="IWM35_001737"
     CDS             complement(3463..5199)
                     /locus_tag="IWM35_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HAP9919587.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNEDLLYVALPVKKSGQLEAILRIAEPTSGFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRACTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLNGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(5196..5900)
                     /locus_tag="IWM35_001738"
     CDS             complement(5196..5900)
                     /locus_tag="IWM35_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9919588.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            6031..6534
                     /locus_tag="IWM35_001739"
     CDS             6031..6534
                     /locus_tag="IWM35_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HAP9919589.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            6494..6832
                     /locus_tag="IWM35_001740"
     CDS             6494..6832
                     /locus_tag="IWM35_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HAP9919590.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            6853..8271
                     /gene="ffh"
                     /locus_tag="IWM35_001741"
     CDS             6853..8271
                     /gene="ffh"
                     /locus_tag="IWM35_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HAP9919591.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            8383..8961
                     /locus_tag="IWM35_001742"
     CDS             8383..8961
                     /locus_tag="IWM35_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HAP9919592.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            8965..9486
                     /locus_tag="IWM35_001743"
     CDS             8965..9486
                     /locus_tag="IWM35_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAP9919593.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(9565..10119)
                     /locus_tag="IWM35_001744"
     CDS             complement(9565..10119)
                     /locus_tag="IWM35_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAP9919594.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
     gene            10372..10647
                     /gene="rpsP"
                     /locus_tag="IWM35_001745"
     CDS             10372..10647
                     /gene="rpsP"
                     /locus_tag="IWM35_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HAP9919595.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            10659..10910
                     /locus_tag="IWM35_001746"
     CDS             10659..10910
                     /locus_tag="IWM35_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HAP9919596.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            11035..11826
                     /locus_tag="IWM35_001747"
     CDS             11035..11826
                     /locus_tag="IWM35_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9919597.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTASTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            11996..12517
                     /gene="rimM"
                     /locus_tag="IWM35_001748"
     CDS             11996..12517
                     /gene="rimM"
                     /locus_tag="IWM35_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HAP9919598.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            12507..13268
                     /gene="trmD"
                     /locus_tag="IWM35_001749"
     CDS             12507..13268
                     /gene="trmD"
                     /locus_tag="IWM35_001749"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HAP9919599.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            13404..13751
                     /gene="rplS"
                     /locus_tag="IWM35_001750"
     CDS             13404..13751
                     /gene="rplS"
                     /locus_tag="IWM35_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HAP9919600.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            14124..14786
                     /locus_tag="IWM35_001751"
     CDS             14124..14786
                     /locus_tag="IWM35_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9919601.1"
                     /translation="MKQNDGRIIWKNQSELKLILTINEFIEKHGITSSRQYQKKLSEN
                     PNSAPSMWFINKKYGSWENLLISIGRENTGYGKWARMSEQELLEIVEAFIKCEKITSQ
                     RMYEQKSVGKNIPSLSTIKKMLGDIRPLFKEKNDGSRFTDFELLLELKNEIIRLKLQD
                     DLSMTKFRKLVQSPKLPSVDTIMKRTNKNWEELMAEIGFDYRRIKIYKQRNNLSKTKK
                     TK"
     gene            14865..18090
                     /gene="fss3"
                     /locus_tag="IWM35_001752"
                     /pseudo
     CDS             14865..18090
                     /gene="fss3"
                     /locus_tag="IWM35_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286924.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
     gene            18204..18518
                     /locus_tag="IWM35_001753"
     CDS             18204..18518
                     /locus_tag="IWM35_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAP9919602.1"
                     /translation="MELKFVVPDMAETFGKIRYAGEGEVLTEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            18531..18905
                     /locus_tag="IWM35_001754"
     CDS             18531..18905
                     /locus_tag="IWM35_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAP9919603.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTVSNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIMDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPDQGNSTNQNEGKK"
     gene            18915..19259
                     /locus_tag="IWM35_001755"
     CDS             18915..19259
                     /locus_tag="IWM35_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HAP9919604.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLTKALTYDEKMFLIMHQGLL
                     EVGNDAEEDFDVLNWLGEEESFFMVIEVNESLCREAETVLEEIGVEMPDAIEVFLKQL
                     VETKQLPIAVND"
     gene            19330..20682
                     /locus_tag="IWM35_001756"
     CDS             19330..20682
                     /locus_tag="IWM35_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HAP9919605.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQELRQFDI
                     SKLSLSTIEIVRSIKDLIYLLGSLVLSGSTMLLYIHFFQDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTKETISYFPKIYYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDIIANRIGIDEVVAESGALRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFVLDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMTRSKAMKEMPNYKTGENYAFLGLPPNFLIFDEYVVYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVDKKFFMKRIKGRGYVDTGGSVISEFYTPLVPKGYDFLREISDIGGLNVNTEG
                     "
     gene            20742..21884
                     /locus_tag="IWM35_001757"
     CDS             20742..21884
                     /locus_tag="IWM35_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HAP9919606.1"
                     /translation="MTFLDLFAGIGGFCLGMEQAGHQCIGFCEIDDFARQSYKAIHDT
                     SKEVEMHDITSVSDEFIQSLGPVDILCGGFPCQAFSIAGKRQGFSDTRGTLFFEIARF
                     AAILQPKFLFLENVRGLLNHEGGATFETILRTLDGLGYDVEWQVLNSKAYVPQNRERI
                     FLIGHSRDACTEQVFPIIGSSPTSDQNIRNLLNINPSNRGMGGQVYGSDDVAPTLTGD
                     EGIKIALPVDDGISVAGMLLGNFEQGNRVYEITGTAPTLSTKQGGTKIMLRKNETEYQ
                     EVKPGNSVNLAFPNSTSRRGRLGKQSVHTLLTGDQQAVVTDQYQIRKLTPRECWRLQG
                     FPDWAFDRASQVNSDSQLYKQAGNSVTVPVIFDIARRLKEVKTDDL"
     gene            21909..22163
                     /locus_tag="IWM35_001758"
     CDS             21909..22163
                     /locus_tag="IWM35_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9919607.1"
                     /translation="MIGKIQSDPELEEELYRLNFDIRKNGVKVPDDPYVIDEETDARM
                     ELNQVISELERIADFAKEPDIRQYLFEIKAELEIEGITAE"
     gene            22419..23603
                     /locus_tag="IWM35_001759"
     CDS             22419..23603
                     /locus_tag="IWM35_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAP9919608.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATTCGLSRPYLNQIENGGV
                     TASTKTMRKIFDQLESFNPELPLTLLFDYVRIRFPTTDARKIIQEILHLKFDYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPDLTKKCQKEECISLFRTFKSYRSGELLKPDDKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAYHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNQKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNQTTYIQDMVNNTRLSDRHKKILEQQSMAIENL
                     II"
     gene            23618..23737
                     /locus_tag="IWM35_001760"
     CDS             23618..23737
                     /locus_tag="IWM35_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HAP9919609.1"
                     /translation="MEIVKDILLVFGGSMLGVTVMCLMHASAAADRAMEKKGK"
BASE COUNT         7871 a         4237 c         5097 g         6556 t
ORIGIN      
        1 agcgcaggaa gtattgagtt agtttgtaaa gtctgcgatc ttcaaggatc gtaaatagct
       61 gattggttgt cccatcaaca caaataaaac tcatggaacc agaaacagaa ttcattgatt
      121 tgaactcatc catacacaac accgtcggaa ggaagttcat acgtggctgg taatgcttcg
      181 ttgcttcacg caagatccgt gtcaccgtcc catccgagac gaagtacttc ttagcgatta
      241 gtttacgtga ttgattttcg tacagttcca tgagaatcac ctgcttcagt tcctttgtta
      301 gttgatgatg ttcatcaact aagtccgttt tcgcagaaaa ggtccttcca caatcatggc
      361 agagaaaacg ggtacgtttt aattctaaaa tagttaactt ttctttgact ttcaacatct
      421 gagtgcgtgt cgtataggaa ccgttgcgga taatacgaac cgatcgacag tgttcacaat
      481 aggaagaaga gtcatccaat tttcctttaa taaactgcgc ttgaacgcct ttatattctt
      541 tttcaatcag ccattcttca tcaaaaaata aatgtttatc tgttaatccg agtaattttc
      601 tagtatgatt atccattgag agactccttt ggtaggatga cgttttggtc gactaaattt
      661 taccaaaagt gagtctcttt ttgagttgaa acgaagaaaa agtgttagta gaaaatcgcc
      721 ttcgattttc caccaacaca aaatattata gaaccctttt tttttgctat atgacaggaa
      781 tgaaaggtgt ttgcaagctt aatgtgagga acactttcca tatctgttct atactggata
      841 ggagaagttt ggaaatatcc agaaaacgaa atcaatcgag gtagccacat gaagaataaa
      901 tggataaatc gaagtaaaaa aaagaaagta acacaaagat catcagataa agtcatccgt
      961 ctttttccat cgtataaaga aggattaacg gcagatcaag tcaaggaacg aatagaaaaa
     1021 ggtgcagcaa ataattcagt cgatcccaca ttcaaaacaa atcagcaaat cgtattggaa
     1081 aacatattta cgtatttcaa tttgatcttt cttatcttag ccattttgct ttgtttagta
     1141 gaatcctata agaacttgac ttttttacca gttatcatag ccaatacagg aatcgccatt
     1201 tttcaggaaa ttcgatccaa aaagatcttg gacgaactaa atgttttaaa tacagcaaaa
     1261 gtcaatgtca tacgagacgg gatagaacaa acaatagata tagaagacct tgtaatagat
     1321 gacattgtta ttttagagac tggatatcaa attccagcag atgcagaggt cgtggaaggg
     1381 aaactgcaag ttaatgaggc tttattgaca ggcgaagcag atgaaataac caaagaaata
     1441 ggtgatccat taatgtccgg cagtttcatt gtttccggaa aagcttacgc ccgattagac
     1501 aaagttggag cagattcgta tatttcccag ctgacagtca aagccaagac aatgggagaa
     1561 ggagaacagt cggagatgat tgcttctctt aatcaattga tcaaatggat tgggatcatc
     1621 attattccta tcggtatcct gcttttttct caaagttact tttttaatgg aaacacgatc
     1681 aaagaaagtg tcgttgcaat ggaagcagca ttgatcggta tgatacctga aggactgtac
     1741 ttgctgacga cgattgcatt agcattaagt gcgactaaac tagcaaagca aagagttctt
     1801 ttacacaata tgaaaagcat tgagacattg gcaagggtaa atgtcctttg tgtagataaa
     1861 acaggaacga tcacagaaaa taaaatgtct gttcaaaaaa tgatcgtctc taaaaaacaa
     1921 gaaataatca gtcagtcaga tcaattggaa gcatgcattg ctgactatgc aaaagcgatg
     1981 gcaggagaca atgcaacaat ggaagctgtt aaagcatatt ttacaaaaac cactgatcaa
     2041 tcctatcacc atgttattcc tttttcttct gtccaaaaat tcagcagtgt agcgttggca
     2101 gacaaggtgt atgttttagg agcgcccgaa atggttttgc gcgatcaatt gccggaatat
     2161 gcggaagagt ttgtttcttt cgctgaaaaa ggctatcgcg tgttggtctt tggtgtgtac
     2221 gatgggattc ttgaagaacc agtattgaca gaagcagtca caccattagg ctatattctg
     2281 attgctaatc ctatcagaaa agaagcaaga gccacatttg attacttcaa aaaacagcaa
     2341 gtagccatca aagtgatttc tggagataat ccgctcaccg tatccaatgt agctgtccaa
     2401 gcagggattt cacatgctga tcagtacgtt gatgcatcta aattatcaga agaagaatat
     2461 gaagcagctg tcgaaaaata tacagttttt ggacgcgtca agccagaaca aaagaaaatc
     2521 ttcgttcaat tgataaagcg taacaataca gtagctatga caggtgatgg cgtgaatgat
     2581 attctagcca tgaaagaagc agactgcagt atagcgatgg cttccggaaa tgaagccgcc
     2641 atgcaggcct cacaagtggt cttacttgat tctgactttt cacgcatgcc agaagttgtt
     2701 gcagaaggac ggcgcgttgt gaacaatatt gagcgatcag ccagcttgtt tttagtgaag
     2761 aatattttct cctttttgct ctcactgttt tcagtgatct ttgctctgac ctatccttta
     2821 gaaccatcac aaatcacatt gattagtttg tttactatcg gacttccatc tttcttgctt
     2881 gctctagaag aaaacaaaaa gcgaatcaga gggaaattca tcatgaatgt gatggaaaaa
     2941 gctgtgccag gtggactaac agatatgatc gtagtcggtg cgttggtgat ctgcggggtg
     3001 actttgaatc taaataaaac agatgtttct acagcatcta ccatgctttt gattgcagta
     3061 gggttcctcg ttctctataa aatctgttca ccgctgaata aattcagaag tcagatcatt
     3121 cttttctgtg ctagcggtaa ctttttttcg gtcatctttt tgcataagct gttttcaatc
     3181 accggtatct cagctgtatc acttttgctg ttgagcattc tatttttctc ggcagattcg
     3241 atttttaggc atttgaccac attggtagaa tatttgtttg aacgtaaaaa tgaaaaagag
     3301 caaaagccgt ttagtcgcgt agctaaaaaa tttctatcga tatttaaacg agaaaattga
     3361 cgaaaagtat ctcaaaaaag agtaaaaaag aacaaccact cgtgtatctt tcgaagcagg
     3421 atgagtcatc caactttaaa acgcacgagc agttgctctt ttttacattt tcggaatggt
     3481 aacggtgaaa gtcgaacctg ttccaagatg actgtctaca gtaatcgtac cattaaggag
     3541 ttgcacgtag tcttttacaa tcgctaaccc taatccggtc ccgcctgaat gtcggcttcg
     3601 cgccttgtct acgcggtaaa aacgctcaaa gatccgttcc tgatcctcaa tgtcgatacc
     3661 tataccaaag tcttggacag aaaacgatag atcatctgtt gcctgatagc ggataataat
     3721 ctcgctgtcg ctttttgaat attggacagc attttcaatt agatttttga taattggata
     3781 aaggatatct gttttagtag taaatggttc gttctttcca atcaatcgaa tcgtcaaccg
     3841 tttcttttcg atgattggct gataattttg aacgatttgc tggaaataag gttctgtata
     3901 aattgtctgg atctcgtaag aattctcgcc atcttttgac agctggatga tttcccgaat
     3961 cagtttatct aaccggatcg catctttttg catgatctca agaaaagaag tcagagtttg
     4021 cgggtcatct tttgcaccat ccagtaaagt ctcggtaaaa ccaatcagtg aagtcacagg
     4081 agtttttaat tcgtgagaga cgttgccaac gaaatccttt tgtagctttt ctaaacgtcg
     4141 cactttcgtt aagtcatacg ccacccctag aatctgtccg gtaccttctg aattagtaaa
     4201 gtagcgcaaa ctcaagtcta atgtctgccg accttctgta agggtgatct cctgatggat
     4261 cagcggatgc tcaggagtca cttgatgaat cagctgaatg agttttggct cttgaatgac
     4321 ttctgtgtac cgctgttcag gttgatagct taccacgttt aaatgtagct gcatctttgg
     4381 attaagcagt agtaaacgag aatcagcatc atcaatcagg aatacgccaa tcatcagttc
     4441 gttcagtaac gtatataatt gctcttctgt ggtagtacac gcccgataca tcttactcat
     4501 ttgttcgctc aacgcattaa tcgtctgata caattcttcc cattgatcgg gtgtttgcat
     4561 gattacttcc gcctgattag gattttgcac catttttttt agtacgggca gcacggtttt
     4621 caatggctgg tttttttgat agatcagata atagatcatt cctgcaagaa caaggaagaa
     4681 agacagaaag aagaaaaaga cccaacggcg aaaactctca gttctcggta aaaaaccgct
     4741 tgtcggttct gcgatacgta aaatggcttc taactggcca ctttttttga ctggtaatgc
     4801 gacatataaa agatcctcat tcaaagtagt acttttgcgc aaagcgctac ctaggttccc
     4861 accattcaat acggctttga tttcgggacg ggagtttcgc tgttcattca atgtttcatt
     4921 gtttgtgtca aataagatct ttccagtagc atccattaaa gtaatccgtt cgttcgactg
     4981 atggacgaat tcttccaaag cagttttgtt ttgcgctgct tggaggtttt cgacatctaa
     5041 ttgccggatc agcagttgtg ctttttttgt caggtagctt tcttgctgtt ccacgatctg
     5101 ctgttgaaaa taatgggaaa tcaattgcca gccaccaaaa aacaaactaa ctaaaagaat
     5161 aagaaagaag agacgttcat aaattttttt ccatttcatc gtttgggttc ctgaaactta
     5221 tagccaaatc ctctaatagt caccaagtac tttgggtgtt ttggatctcg ctcgattttt
     5281 tctcgcaaat gactaacgtg tacatctacg attcggcttt gtcctgcgaa gtcaaaattc
     5341 caaatgcggt ctagcaatgt gtctcggtcg atcacacggt cttttcgttt catgaaataa
     5401 acaagtaatt caaattcttt cggtgttagt tcaatcagat ggtcgtctac ggtcacttga
     5461 taattggtca aatcagcagt gatttgtcca atcgatagat attcaggctc agcttcatct
     5521 tttgattgct tacgaggttc aattcgtctg aatattgctt tcatacgggc taatacttcg
     5581 cgaggactaa atggtttggt caaatagtcg tcagcaccta tttctaggcc aatgatccga
     5641 tccacttggt catctttagc cgtcagcatc aaaatcggtg tttcattttt ttcttgacgc
     5701 agccgctgag tgatctccat gccatccatc gttggaagca tcacatctaa aatgatgaaa
     5761 tcaaatgatt gttctaatgc taattctaat ccttcgcctc catctgtggc actggttacc
     5821 ttgtagcctt ctttttccaa attgaacgtc aagagcgtta caatagaagg ttcatcatca
     5881 acaactagta tttttttcat tcaaatctcc tttaaatagg tttcattctg tttatatttt
     5941 agcatagaat cagtacaaag ggctatttta caaaagaaat aaatggtata ataggaaggc
     6001 actttgctga atagaaagga agagcagtca atgattggaa tcagtgcatg tttaggcggt
     6061 ctggcctgtc gctatgatgg acagaaaaaa gaagtaccag aattgatgga actagttgaa
     6121 actggagaag ctgtcatggt ctgtccagaa gtgatcggcg gattaccaat tcctagagat
     6181 cccgctgaaa tcattggtgg aaatggattc gatgtgtgga aagatcaagc aaaagtatgg
     6241 acgacaagcg gcgaagatgt aacggaagcg tacaaaaatg gcgcgatcaa agcctatcaa
     6301 aaattgcagg aaaaacacat tgatctagtc atcatgaagg aaaaaagccc ttcatgcgga
     6361 acccattcga tttatgacgg aagtttttcc ggaataaaaa aaaatggtgc cggagtggct
     6421 actgcttatt ttattcagca aaaaatgact gtgctctctg atgaagaatg gctgaaaact
     6481 agaggtgagc agaatggaaa tcgaaaagac caatcgaatg aacgcgctct ttgaatttta
     6541 ttcgacctta ttgacagaaa aacaaatgaa ctacatggaa ttatattatg cagatgattt
     6601 ttcattaggt gagatcgcgg aagaatacga agtaagccga caagcagtat atgacaatat
     6661 caagcggaca agcaagatct tggaggatta tgaaaaaaaa ctccatcttt tttctaatta
     6721 tattgttcga gaacaggtat tggaaaatat gactgcatat attacagata agtacccgga
     6781 agataaaaaa ctacttgaat atgtacagca aattcaagca atcgaagaat aaataaaagg
     6841 aatgaagaaa atatggcatt tgaaagctta actgaacgtc tccagcaggc gatgggaaaa
     6901 ataagaaaaa aaggaaaagt atcagaagct gacgtaaaag aaatgatgcg ggaaatccgc
     6961 cttgccttac tagaagccga tgttaacttg caagtagtca aagattttac taagcgcgta
     7021 cgcgaacgtg cggtaggggc agaagtgctg gaaagtttat cacctgcaca acaaatcgta
     7081 aaaatcgttg atgaagaatt gacgatcaca cttggttctg aaacagcaga gttaaataaa
     7141 tcaccaaaaa taccaactgt tatcatgatg gctggtcttc aaggggcagg taaaacgacc
     7201 tttactggta aattagccaa ttatttaaag aaaaatgaaa atgctcgtcc gctcctaatc
     7261 gcaggggacg tatatcgacc agctgcgatc gaccaattga aagtattagg tcaacagcta
     7321 gacgttcctg tatttgatat gggaactgaa gtcagcccgg tagagatcgt ccgtcaaggg
     7381 atggagttag caaaagaaaa gaaaaacgac tatgtattga tcgatacggc aggacgtctg
     7441 catatcgatg aaacattgat ggatgagttg aaacaaatca aagaattgac gcaaccaaat
     7501 gagattttgc ttgttgtcga tgccatgact ggtcaagatg ccgtcaacgt agctgatagc
     7561 ttcaaccagc aattaggtat tacaggggtc gtcatcacta aacttgatgg ggatactcga
     7621 ggcggggcag cactatcgat ccgctcagtg acaggcgcac caatcaaatt catcggttct
     7681 ggtgaaaaat tgactgactt agaagtattc cacccagatc gtatggctag ccgtatcctt
     7741 ggtatggggg atatgctgac attgattgaa aaagcgcagc aagactacga cgagaaaaaa
     7801 gcggaagaac ttgcacagaa aatgcgggaa aattcattcg atttcaatga ctttattgaa
     7861 cagcttgatc aagtgatggg tatgggccca ctggaagatc taatcaaaat gatcccagga
     7921 atgaatcaag tccctggaat cgaaaacgtc aaggtagatc caaaagatgt tgaacgcaaa
     7981 aaagcgatgg tttattctat gacgccagca gaaagagcaa atcctgatct gttgaaccca
     8041 agccgtcgtc gccgaattgc tgccggttct ggaaacagtg ttgttgaagt gaatcgaatg
     8101 atcaaacaat tcaaggaatc aagaaaaatg atgcagcaaa tgtccaaagg ggatatgaac
     8161 atcccaggta tggaccaaat gtttggttca ggcgtcaaag gaaaactagg caaaatggcg
     8221 atgaatcgaa tgatcaagaa aaataagaag aaaaagaaaa aacggaaata agatgaaaaa
     8281 aacagcttcg gtacagactg aaaatgtcta ctgccggagc ttttctatca gcaattttta
     8341 ttctttacta atacaattac tctaaacgaa gaggtggcac agatgagatg tccgtggagt
     8401 gaacaaacag aaagtatgcg gcaatatcat gatactgtct ggggtgttcc tgaatatgat
     8461 gaccaaaagc tgttccgaaa attgatgctt gatattaatc aagccggatt gagctggcag
     8521 acgattttaa ataaaagtga agcattcgat gaagcatatg atcgattcga tatcaaaaaa
     8581 gtcgcaaatt atggagaaac aaaaatcagt gaactaatgg aaaacaaagg aattattcgt
     8641 aaccgaagaa aaatcgaagc agccgtccaa aacgccaaag cagtcttgaa aattcaagaa
     8701 gaaatcggca gtttttcaga gtatctttgg tcgttttcta atggaaaaat cgttgattct
     8761 tcttatcatt cacaagaaga agtcccaaca acaagtcctc tttcagatga attagcaaaa
     8821 gatctaaaga aaagaggatt taaatttatc ggaagcacaa caatctatgc ttttttagaa
     8881 gctgtcggga tcatcaatga tcaccttgac agttgtttcc gcaaagaaga ggtcgtacgt
     8941 atcggcagaa agggcgaata aagcatgcga aaaatcatat tttatggtgc tataagttta
     9001 gatggctatt tggcaacgaa aacggatgat cttcaatggc tttttgacac tccaacaggt
     9061 aagcaaacta cttatgaagc tttttatgaa acaatcgata cgaccatcat gggcagaaaa
     9121 acttatcagg aagcaaaaaa atatttggag accgaagcaa tttatccaga gaaaaagaat
     9181 ttcgtttttt ccacccacgc acaattacaa ttgccagatg ctgaggtcgt aaacgaagat
     9241 cctgttgctt ttctaaaaaa tctacaaatg acagaaggaa aagatatctg ggttgtgggt
     9301 ggcggtcgat tgatcaaacc gctattggaa catcaaatga ttgatgaatg gtatatccag
     9361 attacccctg tattattagg ggacgggatt cctttattcc aaaaaggaac atatgaagcg
     9421 cgttttgaaa tgctagacac gacacgattc ggtgaattta tcgaattgca ttatgtacga
     9481 aagtagaagt aaataaaata aaaaaggcaa ctatatgcag aaatgtcctg tacatagttg
     9541 ccttttttat taccgttatg ctttttactc tgttaatttg ttagaaaaat ctagatacgc
     9601 atctaaaatc gtttggaaga aacttaatgt tccttcaacg atattaccat tttcatcaat
     9661 tagattcgca acgtttccga tatatgcttc cggttgctgc aaggttggca cattcaaaaa
     9721 gacaagtgat tggcgtagat ggtggtttgc gccaaagccg ccgatcccac ctggtgatgc
     9781 agtaacgact aaacctggtt tatgatccca aacactgtgt ccataaggtc ttgagcctac
     9841 atctaaagca tttttcaata ctgctggcac agaacggttg tattcaggag taacgaaaac
     9901 gacaccgtct agtcccctca cttcctcacg aaaacgagtc cattcagcag gagcctgttc
     9961 tggagtttcg agatcctcat tgtaaaatgg cagatcatcg atttttacaa atacactttc
    10021 gtacccttca ggaagcaatt tcgcaaaagc ttctgctact tttttgttgt aagaatcttt
    10081 tcttaaacta ccaataaaaa atccaatttt ttttgtcata cgatttttcc tccttcaaac
    10141 tatctataat aatcttacaa aagataagta actggagcaa ataaaaacac ttactgacaa
    10201 aagctacata gaagatagat tacttatttt taacaaaaaa gaagtctaaa atcttctatt
    10261 ttctaaaaaa aatcgaattt aatcaagctg taaagaaaaa aagctttaca gggtaaaaaa
    10321 aaactgttac actacatctt gtgaataaaa caatggaggt ggaaataaat aatggcagtt
    10381 aaaatccgtt taaaacgtat gggttctaaa aagagtccgt tttaccgtat cgtcgtagct
    10441 gattcacgtt ctcctcgtga tggacgtttc atcgaaactg taggtactta caatcctttg
    10501 aaagaccctg cagaagtagt tttaaaagaa gacttagttc ttgactggtt atcaaaaggt
    10561 gcacaacctt ctgacactgt acgtaacatc ctttcaaaag aaggcgttat gcaaaaacac
    10621 cacgaagcta aattctcaaa gaaataaggt gaccggatat gaaagattta agcgacttag
    10681 tcttaacaat cgttcgtccg ctagtcactt atcctgatca agtcaagtta gacgtcgtag
    10741 agtcgaaaga cttttacgag tacaatctaa cagtggctcc tgaagatatt ggccgtatca
    10801 ttggaaaaca agggcgtgtt gctaaagcaa tccgcacaat cgtttacggt gttcggatca
    10861 atgcaccgaa aaaagtgcgt ttgaatatca tcgatgataa ggaaaagtaa ataaacgtgc
    10921 tgtactcgaa ttttcgagtg cagctttttt gtttttttta ttaattgaag aaatgattat
    10981 ttggcaaaac aagcaagaaa gcgtatcctt ataggaggag ggagtgaaca gccaatgaga
    11041 aagaaaatgt tatttattcc atttgctcta ttgttaagcg cttgtagttc ggttactcaa
    11101 gaatcagaca atacacatct atcaagcagt atatctgaaa ctgttaccag tccagaaaaa
    11161 agtagtacca aaacggcttc caccactgaa caaacaatgg ctacctcaaa taatgagaaa
    11221 aagactgctt tagaccagtt gaaagaacaa cagccaaatg ttcccatgcc tctagatgta
    11281 ccagtctctt caggctatct caatatcgca gcgacacata caaagcaagg ttattctatt
    11341 ttatattaca gaacagatcg tcccctcggg ttgaatgctg acgaattgaa tcaagaaacg
    11401 cctattgcaa cctatctata tcaatatggg tttgcttcta gtcaagaaac aatccaagtt
    11461 ttgcagccat ttgagatcga cacaaatggt cagcaagtgg acttaggttc tcgaatcaca
    11521 gggtatcagc aaggtgcagc aggttcaagt tttcttgaat ggcaagaagg aaactggcgt
    11581 attcgaatcc gtgggaataa tatagaaggc caagatccct tactattagc taaagaaatc
    11641 gtagcttatt tggaagaaaa cagtctacca gcacctgaac aatttggtaa aattactgtt
    11701 gatatgggtg atacgacgaa tcgagccgta gaagtaagct ggcaagaacc gaaaaatgcc
    11761 tatacgatca ctcatcaaga tcctatgtct gcgttgaaga tggcagtttc tatgaagagg
    11821 ttgtgaaatc agcgcgctga tctgagccca tcatttaact ccaagtcaca ggaagtaatc
    11881 aaaaatagca cgccgcattt ttgcataatt ttggcttaat accttaaaag aaaactagaa
    11941 ttgcctcttc acacatgcta aaataaaaca gacaaaaaag aaatggggat ataagttgac
    12001 tgaatacttg aatgtaggaa aaatcgtaaa cacacaaggt atcaaaggcg aagtccgtgt
    12061 tatctcaaca acggatttcc cagaagaacg ttacaaaaaa ggaacggtgc taacactatt
    12121 ccaagacgga aaagcacctg tggaattaac agtcaaaagt catcgcaaac ataaaaattt
    12181 tgacttattg agttttgaag gacatccttc tatcaatgat gtggaaaaat atcgggatgg
    12241 catattaaaa gtgtcaaaag acaactctgt agatctagct gaaaatgaat tctattacca
    12301 tgagatcatc ggtctgcatg tattagaaga tggaactgag ttaggtaaag taaaagaaat
    12361 cttgtcacca ggtgccaatg atgtttgggt cgttcaacgt ccgaaaaaag ctgatatatt
    12421 gcttccatat atcgcttctg tcgtcaaaga agtcgattta gaagctggag tggtccgtgt
    12481 agaaattcca gaaggactga ttgacgatga agattgatgt attgacttta tttccaagaa
    12541 tgtttgaagg tcctatgggg gaatccatca tcggtaaggc tgtagataaa ggattattag
    12601 atatcaatat ctctaacttc cgcgattatt cagataacaa acatcagaca gtagatgatt
    12661 atccatacgg cggtggagca ggtatgctgc tcaaagtcca gcccatctat gataacatcc
    12721 aagccattga aaaagctgca ccggatgtca aaaaaagagt aattcttctt gacccagctg
    12781 gtaaacgatt cgatcagaaa atggcagaag aattttcaca agaagaacac ttagtattca
    12841 tttgtggtca ttatgaggga tatgatgaac gtatccgcac attagtgaca gatgaagttt
    12901 ccttaggaga ttatgtcctt actgggggag aattaggtgc gatggtcatg atcgatgcaa
    12961 cagttcggct tttgccagat gtgttaggga atcagacttc agctcaaacg gattcttatt
    13021 caaccggttt attggaacat ccccaatata ctcgcccggc agaatataaa gggatgaaag
    13081 taccagaagt attgacaaat ggaaatcata aattgattga agaatggcaa ttaaaagaat
    13141 cactaagaag gacctatcaa cgtcggcctg atcttttgga aacacttgaa tggacacctc
    13201 aaatgacgaa atttttagaa gaaatcaaga aagaagaaca agaaaaaaca gcagatgaag
    13261 ccaagtgact ttttctaaaa tcttctttac agtaggttga taatatgtta gtattttatg
    13321 gtgagtgcaa aagcacttaa ctattacgat attccgctgt gagaaaagct cataagaata
    13381 tttggaataa ggagaagaaa accatgaatc cattaatcga agaattaaca aaagaacaat
    13441 tacgttctga catcccagcg ttccgccctg gtgacactgt gcgtgttcac gcaaaagttg
    13501 ttgaaggtac tcgtgaacgt atccagttat ttgaaggtgt tgttatcaaa cgccgcggtg
    13561 ctggaatcag cgaaacttat acagtacgta aagtttctaa cggagtgggc gttgaacgta
    13621 cattcccatt gcatacacca cgtgttgcaa aaatcgaagt agttcgctac ggtaaagtac
    13681 gtcgtgcaaa attgtactac ctacgtgcat tacacggaaa agctgctcgt atcaaagaaa
    13741 tcagaagata aacacggaaa acttccttgg aaatctgaaa tttgtgaaaa gtccttgtat
    13801 atcaagggct ttttcttttt tctctatcac agttttaagc catttttggc tgttttggac
    13861 catttttcaa tcatattccc ttaaaaattg tcctagattg tccgacattg actatcagtg
    13921 tcgtcaaaat agggcaaaat ttagtagtca gcttgacgac aaatataata cgaaaaatac
    13981 caaaacattt accgaattta gcaacttttg aacccaagtt tctcaaaggg tcagctatta
    14041 gcaaaaaaga caaaacatgt acttttctac taaaaagttg atgtgatagg cttctatcta
    14101 aataactatt aggagttttt atcgtgaagc aaaatgatgg acgtatcatt tggaaaaatc
    14161 aaagtgagtt aaaactgatt ttaacaatta atgaattcat cgaaaaacac ggaattacat
    14221 ctagccgtca ataccagaaa aagctatcag aaaatccaaa ttctgcacca agtatgtggt
    14281 tcattaacaa aaaatatgga tcatgggaga atttattgat tagtatagga cgagaaaata
    14341 ctgggtatgg aaaatgggct agaatgtcag aacaagaatt attggaaata gtagaagcct
    14401 ttattaagtg cgagaagata acctctcaac gaatgtacga acaaaaatca gtagggaaga
    14461 atattccttc acttagtacc attaaaaaga tgttaggaga tatacgtcca ctttttaaag
    14521 aaaaaaatga tggatctcga tttactgatt tcgaactgct gttagaacta aaaaatgaaa
    14581 taatccgatt aaaactacag gatgatttat caatgacaaa gtttcgaaaa ctagttcaat
    14641 ccccaaaact accatcagtt gatacaatta tgaaaagaac gaacaaaaat tgggaggaat
    14701 tgatggcaga gattggattt gattatcgaa gaatcaaaat ttataagcag agaaacaacc
    14761 tatccaagac aaagaaaact aaatagtatc actctaaatc agacgttcaa aattaaaatt
    14821 tggacgtctt ttttattacc caaaaacgaa aggagattta ttttatgaaa aagccaagat
    14881 ttaagaattg gcgtttgctc gcaacactga gtctattatg ccaaacaatt ggtggatcgc
    14941 ttggcccaac gattgccttt gctgatgaaa ttactcatcc acaaacggta actgtggaat
    15001 tggatttggc tcaccagtat gctgtcgaag gtacatttag cgatggtcgt cccatgtcag
    15061 aagttaccgt tccgcactat gcagtttaca atggtgtgaa gcaagatatt ttctgtattg
    15121 agccaggggt gccgatttac aatgagttca ctcctggtta tgagaaaaat ccattgcctg
    15181 atatgtcgga gaaagcgaaa ttagtttccg ttttatggaa ggaagctggt acggatgtgg
    15241 atacacatat tgtggcccag aagatgattt ggcaagaagt taatggctac gcttcactca
    15301 attaaacgtt ctgatggtag tgcagtaaat atcgcagcca ttgagtcaaa aatcaatcaa
    15361 gccattgcgg attatcaaaa gaaaccaagc ttccataact ccacagtgaa aaccgtgtta
    15421 ggtcaatcga ctactgtgac agatacgaac ggcttgaatt tatcggagtt tgatgaagtt
    15481 gtcgagaata tcgcaaatat tgattatcgt gtgaacggca atcaactagt gattactcca
    15541 aatgccaatt caaaagagag tggtgaatta accctaaaga agtcagctaa tacaggaaca
    15601 ccggtagctt ataaaatggc tggtcaacaa actttgatgg cgggggcaat tgataagccc
    15661 aatacctaca caatcaaaat tgatgtggaa actgagggtt ctttgaagat taaaaaagtc
    15721 gataaagaaa caggaaatgt tgtaccggga accgttttcc atttagactt tggaaaaaca
    15781 ttacctgcaa aagacgtgac caccgataaa gaaggcattg cgacattgga tgagattcct
    15841 catggcgcga aagtaaccat tactgaaaaa tcagtgccag ctccttatac gattgacact
    15901 acaccaatca ccgcaaccat taaatctggt gagactattt ctgtgacttc aaaaaatact
    15961 cgcgaaaaag gtcaaatcat tttgaataaa atgggggtgg aaacaggaac tgatctttgg
    16021 aacgacaaat actcattagc cggaaatacg tttgccattc gcaaagacag tccaaccggt
    16081 gaaattgtcc aagaaatgac cacggatgaa aaaggtcatg cggaaacacc aaaagagatt
    16141 gccaatgcgt tggaattggg aacctattat gtgaccgaaa ccaaagctag taatggcttt
    16201 gtgaatacct ttaaaccagt aaaagtcgaa ttgaactatg tcaatcaaac cgtggctctt
    16261 gttaccagta acgtaaaagg gcaaaaccaa gaaatcactg gtgaaaccac tttgacaaaa
    16321 gaagacaaag ataccggtaa tgaaactcaa ggtaaagctg agtttaaagg agctgaatat
    16381 actctcttta ctgcaaaaga tggtcaagcg gtcaaatgga gtgaggcttt taaaccagag
    16441 ttagtgaagg gaacgaaagc ttctgatgaa acggtgactt tgactttaga tgaaaagaat
    16501 caagttgctg ttaaacactt agcgattaat gagtattact ggcaagaaac caaagcacct
    16561 gaaggatata ccttggatga aacaaagtac cctgtttcca tcaaaaaggt ggatgataac
    16621 gaaaaaaatg ccgtgattac ccgagatgtt acggcaaaag aacaagtcat tcgctttggc
    16681 tttgatttct ttaaatttgc tggatcagct gctgggactg ccgaaactgg cttcaatgat
    16741 ctgaccttta aagtatcgcc attggaagga accagtgaga ttacaggtgc cgaagatgaa
    16801 gcaattacag cttataacga gcaactaggt tttgatggct atggcaagtt tgaaaatctt
    16861 ccttatgggg attatttact tgaagaagta gaggctccag aaggatttca aaaaattacg
    16921 ccattagaaa tccgttctac ttttaaagaa aataaagcgg attacactaa gagtgaatac
    16981 atttttacga ttaccgaaaa aggacaaaaa caaccaatta aaacggtgac cgttccttac
    17041 gagaaactga cgaataaagc attttctgtt agcttgaatc gtttgatgct ctatgattta
    17101 cctgaagaag aagatagctt aacttctctt gggacttgga aagacggagg caaagaattg
    17161 acagaccttg attctaccga gctagttgat aaattgagct ataacttgca tgaaatcaaa
    17221 gaagactggt atgtggtagc gcaagccatt gatgtggaag cgacaaaagc tgcccaagaa
    17281 aaagatgaaa aagccaaacc agtggtaatt gccgaaacaa ccgcaacgtt ggcgaataaa
    17341 gagaaaacag gaacttggaa aattttgcat aaattaaccg cagaacaagt tttggataaa
    17401 accatcgtct tattcaacta tgtgtatgaa aacaagaaac cctatgaagc aggtgatgat
    17461 ccagtagcga aagatgctag cttgaacaat caagcccaaa ctgtcaattg tacggtggaa
    17521 cgccatgttt ccatccaaac aaaagcccac ctagaagatg gttcgcaaac ctttactcat
    17581 ggggatgtgg tggatatgtt cgatgatgtg tcgattaccc atgatgtact ggatggctca
    17641 aaagaagctt ttgaaacaat tctgtatgca ttacttccgg ataatacgac caaagaaatt
    17701 tggaaatctg gcaaaattga ttatgaagtg aatgacaaag aattcactaa aaccgtactt
    17761 gcggaaaaag tggacacaag taaatatcca gaaggtacaa gttttacctt caaagaaatt
    17821 aactatggca aagacggaaa catcaatggg aaacacaatg aggatctaaa agaaaaaact
    17881 caaactttaa cacctaaaaa agttccgaca acaccaagtg caccagaaca accggaaata
    17941 ccaactgttc caagtgactc tcaagaatct agtcctacag taaagacatt cccacaaaca
    18001 ggggagaaaa attctaatgt tctgctgttt attgggttta ccttgatctt tgcgactgct
    18061 gggtattatt tctggaatcg ccgaaactaa ggtgatgtga tgaaacaact aaataggaaa
    18121 aaccaacgtg cgcgaccgcc tcctaaaaaa agtggtcgcg ctatttttgt atccaaaact
    18181 aaaaatgaaa agaggaaaag aagatggaat tgaaatttgt tgtgccagat atggccgaaa
    18241 cctttggaaa gataaggtat gccggcgaag gcgaagtttt aactgaagga tatgggcgga
    18301 atactacagt aattggtcgt agttatcatt tgtattccag taagcaacga gccgatgata
    18361 ttgaagtggt ggtagctgcg gaagccggcg aaaaggattt tgatcaagat caaccactaa
    18421 aagccgtgaa tccccatttg gttgccaaag gctatgagat tgagaatcgt gggtttaccg
    18481 actacgtgtt gtatgtcgat gatttagtga aagcatagga ggaaaagaag atgagattaa
    18541 cagaaggtat tgttgtagat tcaggactaa cgtttgggaa attacgattt tccgcattac
    18601 gccgagaagt acggaagcaa aatgaggatg gaacggttag taacgaggta aaagaacgga
    18661 cttataattt gaaatcctct gcgcaaggtc gaatgatcca agttagtatc ccagcaaatg
    18721 tacccttacg cgagtttgcc tatgacgcag aggtagagtt ggtcaatcca atcatggata
    18781 cggtcgccaa ttatgttttc cgagaaggaa ctactgtcaa ttggttcatt aaggccgatg
    18841 atttagtctt gaaacgacaa ccagatcagg gaaattctac aaaccaaaat gaaggaaaga
    18901 aataggtgaa gcgtatggaa attaatgttg aacatatttt agattgtctc gatcaatatg
    18961 gaaaaggtga gctaacggag gaacaactca ctaaagcatt aacctatgat gaaaaaatgt
    19021 ttctgattat gcatcaaggg ttactggaag taggtaacga tgccgaagaa gattttgatg
    19081 tactgaattg gttaggggaa gaagagtcct tctttatggt tatcgaagta aatgaaagtc
    19141 tatgtcgaga agccgaaacc gtattggaag aaattggtgt ggaaatgccg gatgccattg
    19201 aagttttttt aaagcaacta gtggaaacga aacaactccc tatagcagta aatgactagc
    19261 ataactgctt gaaattggat caatgaaatc ctatgaagct tttttcgatt ggaggtggaa
    19321 accataaaaa tgtacaaagg gcatcgtata cgggcaggag atcagcactt ggtttatcac
    19381 tttgttcttg gctggctact cgctctgttc atcggttgga tgagcgtctt ttattttcaa
    19441 gagcttagac aatttgatat ttctaagcta tcactttcta ctatagaaat cgttcgttct
    19501 ataaaagatt taatctattt gctaggaagt ctcgttcttt caggaagcac gatgttgctt
    19561 tatatacact tttttcagga tcattggcgc agtttgtggc atcgacaaaa gctagcccgg
    19621 atgattctag aaaatcattg gtacgaagta aaacaaactc agagtgaagg atttttcaaa
    19681 gacctcaata gtagtcgaac caaagagaca atcagttact tccccaaaat ctattaccgt
    19741 atgaaggatg gtttactttc tattcgcgtt caaatttcac tgggaaaata tcaagatcag
    19801 cttttaaagc tggaaaagaa gttagaaagt ggtctatatt gtgaactagt agaaaaggaa
    19861 ctcaaagact cctatgtgga gtacacttta ctgtatgata taattgccaa tcgaattggg
    19921 atagacgaag tagtggcaga aagtggggct ttgcgattga tgaaaaatca agtatgggct
    19981 tatgattctt tacctcatat gttaatcgct ggcggcacag gtggtgggaa gacttacttt
    20041 ttactcacta tcatcgaggc tttattaaaa tctgatgccg agctttttgt tcttgatccc
    20101 aaaaatgcgg acttagccga tttaggtacg gtaatgcctc atgtctattc ccaaaaggag
    20161 gaaatttctg cttgtgtgga agatttctat gagcgcatga tgactcgtag caaggcaatg
    20221 aaagaaatgc ccaactacaa aacaggagag aattatgcgt ttcttggact tccaccaaac
    20281 tttttaatct ttgatgaata cgtggtttat atggaaatgt taacgacgaa ggaaagtgcg
    20341 gtgattttga ataagctcaa acaaatcgtg atgttaggtc gtcaatctgg tttctttctg
    20401 attctagctt gtcaaagacc ggatgcgaaa tatttaggag acgggattcg cgatcaattt
    20461 aatttccggg tggctttggg tcgtatgagt gaactgggat attccatgat gtttggcgag
    20521 gttgataaaa aattctttat gaagcgtatc aaaggtcgag gctatgtaga tacgggaggg
    20581 agtgtgatta gtgagtttta tacgccactt gtaccaaaag gatatgattt cttaagggaa
    20641 attagcgaca ttggtggatt aaacgttaat acggaaggat aaaataatag tatgtagcct
    20701 aggagtgtca agaatcctgg gcttttttta cgaggtgatt tatgacattt ttagatttat
    20761 tcgctgggat tggtggcttt tgcttaggta tggaacaagc gggtcaccag tgtatcggct
    20821 tttgtgaaat tgatgatttt gctcgccaga gttataaggc aatccatgat acaagtaagg
    20881 aggtggaaat gcatgacatc acaagtgtat cagacgagtt tattcaatct ctcggaccag
    20941 tggatatcct ttgcggtgga tttccgtgcc aagctttttc aattgcggga aagcggcaag
    21001 gattttccga tactcgaggt actttattct ttgaaatcgc tcggttcgcc gctattctcc
    21061 aacctaagtt tttattcctt gagaacgtcc ggggattact caatcacgaa ggaggggcta
    21121 cgttcgagac gatcctccga acgctggatg gattggggta tgatgtggaa tggcaagtgc
    21181 ttaactcaaa ggcctacgtt ccccaaaacc gtgagcggat cttccttatc ggacattctc
    21241 gagacgcatg taccgaacaa gtatttccta tcattggatc gtctccaaca tctgatcaaa
    21301 acatcagaaa cttattaaat atcaaccctt ccaatcgagg aatgggggga caagtctatg
    21361 ggtcagatga cgtagcgcct accttgaccg gtgatgaagg aatcaaaatc gcattgccgg
    21421 tagatgatgg aatttctgta gcgggtatgt tactgggaaa ttttgaacaa ggcaatcggg
    21481 tttatgagat aactggaaca gcgcctactt tgtcaacgaa acaaggcgga acgaagatca
    21541 tgcttcggaa aaacgaaaca gaatatcaag aggtgaagcc agggaacagt gtgaacctgg
    21601 cttttcctaa ttccacaagt cgtcgaggcc gtctgggaaa acaaagtgtg cataccttat
    21661 taacaggaga ccaacaagca gtcgtcacgg atcagtatca aattcgaaag ttaacaccta
    21721 gggagtgctg gcggcttcag ggctttccgg actgggcttt tgatcgagct tctcaagtca
    21781 attcagatag tcagttgtat aagcaggctg gaaattctgt aacagttccg gtcatttttg
    21841 atattgctag aagattaaag gaggtaaaaa cagatgattt atgatgagtt gattggggaa
    21901 tctactgggt gattggcaag attcagtcag atccggaatt ggaagaagaa ctgtaccggc
    21961 tcaattttga tattcgcaaa aacggggtga aagtccctga tgatccgtat gtaattgatg
    22021 aggaaacgga tgctcgaatg gagctgaatc aagtcatcag tgaattggaa cgaattgcgg
    22081 actttgctaa agaacctgat atccgccaat acttgtttga gataaaagca gagttggaaa
    22141 ttgagggtat taccgcggag taacggccaa ataaaccact gataggcact gaccgcgggg
    22201 gatttgcgaa gcagaaatcc tccgtggtgc tgtgcctgtt tatggacggc gttagccggc
    22261 catgatgtct caccccccgt atctaacagg ggggtacaaa aacacaaaaa ataagcaagc
    22321 aacgataagg aatggctgtc ctatcaagga taataagcaa tttgtgtaga tgttaactga
    22381 tttgaaaaag ttaacacctt aaatttggag gtgagaactt ggcacaaagg aatttagatt
    22441 atcggctatt aaaagaccgg cgtaatgaat atggcgtttc acaaaataaa ttagctacga
    22501 cttgtggact tagtcgcccg tacttaaatc agatagaaaa tggtggcgta accgcatcga
    22561 caaaaaccat gaggaaaatt tttgaccaac tggaaagttt caatcccgag ttacctttaa
    22621 cgttactgtt tgactatgta aggattcgct ttcccacaac ggatgcgagg aaaatcattc
    22681 aagagattct ccatttgaaa tttgattata tgctccacga agattacgcc ttttactcct
    22741 atcaagaaca atatgttatg ggagatattg tggtgatgtt gtcacatgaa gaagataaag
    22801 gcgtcctttt agaattaaag ggtcgtggtt gtcggcagtt tgaaactttt ttactcgccc
    22861 aaaagcgtag ctggtatgac tttttcgaag attgtctaaa agccggtggc gtgatgaaac
    22921 gcttagacct ggcaatcaat gatcgagttg gcctgttaga tattcctgat ttaacgaaaa
    22981 aatgtcagaa ggaagaatgt atctccttgt ttcgtacctt caaaagctat cgttccgggg
    23041 aactgttgaa acctgatgat aaagatggta tgggcaatac tttgtatatt ggcagtctta
    23101 agagtgaggt ctatttctgt ttgtacgaaa aagactatga gcaatatatc aagttaggga
    23161 ttcctttaga ccagaccgaa acgaaaaatc gttttgaaat ccgactgaaa aatgatcgag
    23221 cttatcatgc aattcaagat ttgttaaaag gtcgcagtat tgaaagtacc acattttcca
    23281 ttattaaccg gtacttgcga tttgcggata aggtggaagg caaaaggcga actaactggc
    23341 ccttgaatga acaatggggg cgcttcattg gacggaatca gaaggaaatt caattaactt
    23401 ctgaaccgaa gccttatacc attgaacgaa cgttgaactg gcttggacga caagtcgccc
    23461 ctacgtggaa gatggccaag gagttggatc ggttgaatca gacaacgtac attcaggata
    23521 tggtaaataa tactcgacta tcagaccggc acaaaaagat tttggagcaa caaagtatgg
    23581 caattgaaaa tttgattata tgaaaagagg aatcgtgatg gaaattgtga aagatatttt
    23641 attggtgttt ggtggcagta tgcttggtgt tactgtgatg tgcttgatgc atgcaagtgc
    23701 agcggcggac cgagcaatgg aaaagaaagg aaagtgatga aatgaatttc ggtcaaaatt
    23761 t
//
LOCUS       DACDXO010000040        23648 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E23605_2017
            SAMN12639989-rid9881463.denovo.040, whole genome shotgun sequence.
ACCESSION   DACDXO010000040 DACDXO010000000
VERSION     DACDXO010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639989
            Sequence Read Archive: SRR10023389
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23648)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23648)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741914.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:42:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,972
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 33 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 17 of 73
            Pseudo Genes (multiple problems)  :: 10 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23648
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639989-rid9881463.denovo.040"
                     /isolate="E23605_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(55..789)
                     /locus_tag="IWM35_001761"
     CDS             complement(55..789)
                     /locus_tag="IWM35_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HAP9919610.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            1116..1526
                     /locus_tag="IWM35_001762"
     CDS             1116..1526
                     /locus_tag="IWM35_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HAP9919611.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     AGKTAEGVEALLGYVRKKKAERVSVNEMEENQID"
     gene            1519..2220
                     /locus_tag="IWM35_001763"
     CDS             1519..2220
                     /locus_tag="IWM35_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HAP9919612.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            2220..3806
                     /locus_tag="IWM35_001764"
     CDS             2220..3806
                     /locus_tag="IWM35_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAP9919613.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKQKLLKQ"
     gene            3825..4046
                     /locus_tag="IWM35_001765"
     CDS             3825..4046
                     /locus_tag="IWM35_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HAP9919614.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            4043..4804
                     /locus_tag="IWM35_001766"
     CDS             4043..4804
                     /locus_tag="IWM35_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HAP9919615.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEEISLRFPETFAYALGDVRQMKELQQAVSKCQNHFGEVTILLNTAGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRQKSGTIINMASIAGLIAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            5184..5693
                     /locus_tag="IWM35_001767"
     CDS             5184..5693
                     /locus_tag="IWM35_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HAP9919616.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANIASPLGLIDVAVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(5768..6307)
                     /locus_tag="IWM35_001768"
     CDS             complement(5768..6307)
                     /locus_tag="IWM35_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HAP9919617.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVGVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(6447..7058)
                     /gene="rpsD"
                     /locus_tag="IWM35_001769"
     CDS             complement(6447..7058)
                     /gene="rpsD"
                     /locus_tag="IWM35_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HAP9919618.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            7374..8171
                     /locus_tag="IWM35_001770"
     CDS             7374..8171
                     /locus_tag="IWM35_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-nitrite transporter"
                     /protein_id="HAP9919619.1"
                     /translation="MNPVSPLFEKIDASIEKKANLFENSFSRYAVRAVLACLFLTLGT
                     AVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAELGTSNMLYMTVGVFRQRLA
                     LSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNFMLESIAVKLAKPSATILV
                     EGMFANIVVNTAVLISMRMKEDVGKVITVVFIIFIFAFLGYEHVIANFPAFTLTYFAS
                     HGQMDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQSKSSYVD"
     gene            8199..8834
                     /locus_tag="IWM35_001771"
     CDS             8199..8834
                     /locus_tag="IWM35_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HAP9919620.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVHYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            8854..9627
                     /locus_tag="IWM35_001772"
     CDS             8854..9627
                     /locus_tag="IWM35_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP9919621.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            10035..10832
                     /locus_tag="IWM35_001773"
     CDS             10035..10832
                     /locus_tag="IWM35_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HAP9919622.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            10810..11223
                     /locus_tag="IWM35_001774"
     CDS             10810..11223
                     /locus_tag="IWM35_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9919623.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEKEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            11207..13852
                     /gene="mutS"
                     /locus_tag="IWM35_001775"
     CDS             11207..13852
                     /gene="mutS"
                     /locus_tag="IWM35_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HAP9919624.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            13870..15978
                     /gene="mutL"
                     /locus_tag="IWM35_001776"
     CDS             13870..15978
                     /gene="mutL"
                     /locus_tag="IWM35_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HAP9919625.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRETGTFFVEELKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            16000..16560
                     /locus_tag="IWM35_001777"
     CDS             16000..16560
                     /locus_tag="IWM35_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HAP9919626.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(16645..18633)
                     /gene="treP"
                     /locus_tag="IWM35_001778"
     CDS             complement(16645..18633)
                     /gene="treP"
                     /locus_tag="IWM35_001778"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HAP9919627.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            18856..19569
                     /gene="treR"
                     /locus_tag="IWM35_001779"
     CDS             18856..19569
                     /gene="treR"
                     /locus_tag="IWM35_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HAP9919628.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            19646..20092
                     /gene="msrB"
                     /locus_tag="IWM35_001780"
     CDS             19646..20092
                     /gene="msrB"
                     /locus_tag="IWM35_001780"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HAP9919629.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGEFKSLVE"
     gene            20262..20864
                     /gene="ruvA"
                     /locus_tag="IWM35_001781"
     CDS             20262..20864
                     /gene="ruvA"
                     /locus_tag="IWM35_001781"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HAP9919630.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAIEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            20877..21878
                     /gene="ruvB"
                     /locus_tag="IWM35_001782"
     CDS             20877..21878
                     /gene="ruvB"
                     /locus_tag="IWM35_001782"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HAP9919631.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            21907..22485
                     /locus_tag="IWM35_001783"
     CDS             21907..22485
                     /locus_tag="IWM35_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HAP9919632.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            22537..23019
                     /locus_tag="IWM35_001784"
     CDS             22537..23019
                     /locus_tag="IWM35_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HAP9919633.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIALEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            23136..23510
                     /locus_tag="IWM35_001785"
     CDS             23136..23510
                     /locus_tag="IWM35_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9919634.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
BASE COUNT         7728 a         4272 c         5066 g         6582 t
ORIGIN      
        1 ataaaaaaac ttggtacgca atcgctgtac caagtttttt tatttagctt ttgattaagc
       61 cataaataat aatttatata agaatactgc tacgacacca gcacagatag gtgctacaac
      121 tggtacccat gcatatcccc attgagaaga acctttatgt ttcaatggaa gaagtgcgtg
      181 tacaagacgt ggagcaaagt cgcgggctgg gtttagtcca ggacctgtag gaccaccaag
      241 tgaagcaact aatgtccata caaggaaacc aagtgctaag tgagctgtac caagattatc
      301 ttggaagaat ggtgcttttg taatacctag ggcaccaaag aataaaacga atgaaccaaa
      361 gaattcattg acgaaaccat tgaatttaga accaacgctg ttgattgttg agaatgtacc
      421 taacacatgg tttacgtctt ctgtttggtc gtagtaaggt ttgtaacaag ctactacgat
      481 caattgtcca atcgctgcac caatcaattg tgcaatgata taaggaacaa cttcagccca
      541 agggaataga ccattagatg ctaaagcaat tgtaaatgct gggttgatgt ggttaccgct
      601 gattccacca aaaatcattg ctggcatcat aaccccgaat ccatacccga cagcaatcag
      661 catccagtca ctgccatatc cttttgtacc ttttaaatct acgtttgcta cagcaccgtt
      721 acctagaacg attagtaatg ctgtaccaat catttccgcc gcaagacggg tcattagtga
      781 gtaatccata aatacctcca aaagttataa aaatgcaaaa aaaaaagaaa catcaacacg
      841 aaaagcatcg gtgcagtgtt tcgtgtcgga aggatttctg cagtcttaac aaatgtgaat
      901 aaaaaaatga attccattac ttataagaaa ccagaccaac agtaaaactc caactctttt
      961 taaaagttct ccacaaatca ccaagcttaa ctataaatat accaaaaaaa aaacgttttt
     1021 tttctcaaaa attgtatctt agaaacagca tgttttgaaa ctttcagaaa aggtttgtta
     1081 gtaaaatgaa agctatacgt aaggagtgtt gtttgatgaa attttttcaa gagtttttca
     1141 ttattatttt cttttctgta ttaggagaag gcatacggat ttttgctcat ttacctattc
     1201 ctggaagtat cttaggtatt ctttttttat ttttagcatt tgaaatgcat cttgttgatc
     1261 ctgaaaaaat cggagcaact ggtgattttt tattgaataa tctagccatt ttatttgttc
     1321 ctgctggtgt aggtttattg gaatattttg atgatattgc ttcgatatgg cctgttttac
     1381 tgggagcagt cgtcgtgtgt tccatcgtca cgatggttgc agcgggaaaa acggcagaag
     1441 gtgtagaagc tttgctgggc tatgtccgta agaaaaaagc agaaagggta tctgtaaacg
     1501 aaatggagga gaatcaaatt gactgagttt tttgtgaatc cattatttgg cttaagtttg
     1561 actttaggag tgtacgtatt atctgacaaa tttttgcgga aatataaact gcctgttttg
     1621 aatcctctcg tattatcgat tgttgttatt atcttatttt tgcatttttc tgggatcagc
     1681 tataaagact ataatatcgg gggcaatttt ttgactatgc tgattactcc tgcgacagtg
     1741 gcacttgcca tccctcttta taaaaacttc cgtttactga aagagaattt ttatcctgta
     1801 atggctgcga ttttagcagg aatagtcgca aattgcttgg ttagtgtagg gttcggttac
     1861 ttcttctctc ttcataaaga gatggtgatc tcattactac ctaaatctgt aacgacggct
     1921 atttctgtcg atttatctca tacgatgggc ggggtgaatg ccgtgacact tgctattgtt
     1981 gtctctacag gtatttttgg ttcgctgatc gccactcata ttttccgcct attcaagatc
     2041 gaaagtccag ttgctcgcgg tgtggcgtta ggaagtactt ctcatgctat tggtacagca
     2101 aaagcaatcg agattggtga aatagaagga atcatttccg gacttgcgat atgcgtcaac
     2161 ggaatattga ccgttttatt gctaccgtta ttctttcagc cttttgctgg cttgttttga
     2221 tgcgaaaacc actattttat gctacaataa ccaaaacgtt cgagcataaa aaaggaggga
     2281 aagacgtgta tttgtcttcc gagtatctta aacaatttga taaagatcta tattataaga
     2341 ttctcctcct agaaagtttt gaggatcaag cttggcatac cgctgcacaa ttagcgcaag
     2401 ttgttcagct ggatgcccgc agcgtgtcga aatacctgaa cgagctgtct aaaaactatc
     2461 agcagtttag cggtaagaca caccctttgt ttacaaaaaa tcaccgttct ggttataact
     2521 tttatgatac gctcgattca attgaacatg agcgtttttt gatctatctc gtgcaaagta
     2581 cgctgaaatt ccagttgctg cacgacattt tttttgaaga attccataca atgtaccaat
     2641 ttgcgcaaaa gcattacatt agtgaatcta ctgctcatcg aaaaatcaat gaatggaaac
     2701 agcaactgca aacttacgga attcggctgc aacgaggtac atatatcgct caaggcgaag
     2761 aagaaatcat tcgactttac ttgcatatga cattctggca gttgtttcga gggaaaatct
     2821 ggccatttga aacgatttct caaatggacg taaagaatat ggcagaacat atcatggctt
     2881 tttttaatgt tcgtctgaac gagatcaaaa aaagacgatt ggaatatatg ctaggtgcct
     2941 tttttttaag aaaatcacag aagcattatg ttgtattgaa tgagaaaaaa agacggctga
     3001 tttctgacaa tctgttgttc cagcgatttt gccaggtcat ggagccggta tttcctaatt
     3061 attttcaagt agaagacgag ttaggcgcat tatttcttgt actgatgact cgtgaagaat
     3121 attatagtga tcctaagata cggaagaaaa tttttgattt tcatcaacaa gcaaaaacac
     3181 cgccctttac agccctttca gaagcaaaag ccgctctttc tctttatcag gaagagcagg
     3241 ggctgccagc agaaaatcta accttcgagg cagaaaacta tttattcagc agtcattttt
     3301 tcgcctatct ttttcctaat gcgaaagaaa cgattgatgg caatagtagt gattttatta
     3361 atcatttagt tattgaaaac aaggaattga aacaatggct cgtacatttt tttgaaagta
     3421 gacacaaaca tcccaatcat ctggcattta aaaatcatgc gtttttgatg gggcggtatc
     3481 tgactgtgtt taaaacatta ggagccttta ctccacagct tccaaagatc acgatccttt
     3541 taatgacaga tttcccatta tttgaagagc aactgttaga ggagggatta cggacatttt
     3601 tccgtaatga gtatcagctg atttttctgc caacagatta tcgggggaga gaagttgatc
     3661 ttctgatttc gacaagtaaa gtccaccgca aaccatgggc ggatcttgac tattttattg
     3721 tgactgaaga actaaaatta attgattaca tccaactatc tcaaaaattt gaaatgattc
     3781 aaaaacaaaa actattaaaa caataacaaa gaaggagaaa aaagatgaca tttgaagaaa
     3841 ttttaccaga attgaaaaaa ggtgcaaaga ttattcgtaa aggatggagt ggatttgaac
     3901 tttatgttgt tctcgtatcc gatgatgaat ttgatggctg tccggtgacg ccgtacttat
     3961 tgatcaaaac aagtgacgaa ggtttttcca gcttcgctcc tacagtttgt gatattttag
     4021 cagatgattg ggcgatcgta gaatgaaaaa ccagtatcca gatctagcta aacaggttgt
     4081 atttatcaca ggagctgctt ctggaattgg tgctgcgcaa gcccgagcgt ttcttgaaca
     4141 agatgctttt gtatttggat tagatcaaaa gttcggtaaa atggaagaaa tttctttgcg
     4201 atttcctgaa acttttgcct acgcactagg tgatgttcga caaatgaaag aactccaaca
     4261 agcggtatcg aaatgccaaa atcattttgg tgaagttacg attttactga atacagcggg
     4321 gatattggat gcctacaaac ccttgatgga aactgacgaa tcgctgtggg atctgattta
     4381 tgagacgaat gtgaaaagca tgtatcagct gacaaaaatc atccttcctg atatgatccg
     4441 ccaaaaaagc ggaacgatca tcaatatggc ttcgattgct ggcttgattg ctggtggagg
     4501 tgggatcgct tatacaagcg cgaaacatgc gattgtcgga tttaccaaac aactcgctct
     4561 ggattacgca ggacagggta tccgagtaaa aggaattgct ccaggagcca tccaaacacc
     4621 aatgaatgcg gctgattttg aagggaaagg tgagatggca gagtgggtgg caaatgaaac
     4681 accagtcaag cgatgggcac agccggaaga agtagctgag ctgacgttat ttcttgcctc
     4741 tcctcaagca agctacatac aaggaacgat catcccaatc gacggcggat ggttgttaaa
     4801 ataaccttgt gtttttttca agcttctgta tgataaaact aaagagagaa acaacggttg
     4861 atgataaaaa aagtcgatga ctgttcccga cattcgatgc cgaacagcca gttcaaaaaa
     4921 ttctttttaa taatcgttaa tattcgaaac taagcggctt ttcacgacct cttgatataa
     4981 acgatattca aaagcttgca actgcggtaa caagtccagc caaatgaaaa atataaggag
     5041 ctatttttat ttgaccattc ttattacttg ttgcagggca gctttttcac taccttctgc
     5101 gacacttcag ttggaatttt cttcccatcc tttggtcgcg gtcattacca agataaaatt
     5161 atcttgcaaa ggagaaatag aacatggaaa aaaataatac ccgttcagcc aaacgctgga
     5221 caacgacaga tgtgactcgg atggcgctag tcactggttt atatgtggca gtgacgctgg
     5281 ttttgtctgt ctttagcttt ggagtgattc agattcgtct ctcggagatg ttcaactatc
     5341 tgtccttgta taacaaaaga tacatcgtcg cggtaacagc gggagtagct ttggctaata
     5401 ttgcttctcc tcttggactg atagatgttg cagtaggaag cgtcagcacg ctgcttgtat
     5461 tgcttatcaa ctataaaatc actagtcgca tcaaaaacat gaaaatcaaa atggccgtga
     5521 cagcatgcgt atttgctttt tccatgttta cagtagctgg tcagctaacg attttatatc
     5581 aactaccttt tttcttgaat tggtgggtga ttgctttagg agaattggca tcaatgattg
     5641 tcggtgggat aattatttat tggatcggta aaaaagtgga tttgtcaaaa taaagatctg
     5701 tttaatttaa aatagggcag catggaagag actgaatgtt gagtcttctt ccatgctgct
     5761 tttttgatta atttgtatga agaaatcgat ttagccgttt tttcatcaag ataccgatat
     5821 aagctgctcc agttgccttg attgcttccg gcagtaaaaa cggcagtagt ccactggcaa
     5881 atgcagttgt aaaagtaaga tcaccactta ttttcagcca gatcgttccg cagatcaatg
     5941 cagcaagaga gcccattaga ttggcaaaga ttgcatataa gtaatgagca tccgttcttt
     6001 cgatcatcca accagttatc cacgtttgta agagaaatcc aatcaaatat ccgccagttg
     6061 gcccaaataa aacagctata cctccagtca tccctgaaaa tacaggaaga ccaatcaagc
     6121 caagtaaaag ataaactcca acagatatca atcctagttt tcttccgaga aaagttgctg
     6181 ttaatccaat agcaaatgtt tgccctgtca aaggaaccaa accaaaagga atggtcatct
     6241 gagaaaggac agaaataatc gcggcaaatt ccgcagcaag tatcaattct ttcaatgttc
     6301 ggttcataac gccctctttt cgttaactta atatttttat ttggttaacg aaattgtaaa
     6361 acaaagtgat tactttgtaa atagaaaata taaaaaaaga ccagctatcc aatagatagc
     6421 ggtctttttc aagaaagctt ttgaaattat aagctttggt tgtagtattc aacgacaaga
     6481 gcttcgtcga tttctggata tagttcgtca cgttctggta aacgagtcaa tgaaccttcc
     6541 aatttttcag cgtcaaagct tacgaatgct gggcgtccaa cagttgcttc tacagcttct
     6601 tttactgtta cgatgttttg tgatttttca cgaactgaga tcacttgacc cacttctacg
     6661 tggtatgaag ggatatcaac gcgtttgcca tcaactaaaa tgtgaccatg gttaactaat
     6721 tgacgtgctt gacgacgagt cgttgcaagt cctaaacggt aaacaacatt gtctaaacgt
     6781 tgttctagta agatcatgaa gttaacacca tgtttacctt ctttgatttt gctagctttt
     6841 acgaacaagt tacggaattg acgttcgttc attccgtaca tgtgacgaag tttttgtttt
     6901 tcagttaatt gcatgccgta ttctgattgt ttgccacggc tattaggtcc atgttgacct
     6961 ggtgcgtaag ggcgacgtgc taattctttt cctgtgcctg acaatgagat gcctaagcga
     7021 cgagaaattt tccatgatgg tcctgtataa cgagacatta aaatttcctc caatgaatat
     7081 aagtttttgg agtaaaataa tccgttgaaa aattcataat catatagttt gttcttcaat
     7141 cttcaccttt gcagccgcgg ttacacaatc gaacccgaga cggggcaaca aactagggat
     7201 gagttattat ttttctgctg cattatttta cacaaattac agtatatgat agatcatcca
     7261 cgaatgtcaa gataattagt acataaaaac gctttacaaa cctaggctgt ctttataaaa
     7321 tgagagggta tgtgcaaatg gaaaaaagcg aaggaagttt aaggagaaaa tcaatgaatc
     7381 cggtatcacc attatttgag aaaatcgatg cttcaattga aaagaaggcg aatctttttg
     7441 aaaacagctt ttcacgttac gctgttcgag cggttctcgc ctgtttgttt ttgactttag
     7501 gaacggctgt ggcatttgcg atcgcgatga agggggaaga tatcgcgcat ggtctaggaa
     7561 agattttata cgcatttatg ttcagctggg gtctggtcat gattctatac atgaacgccg
     7621 aattaggaac atcgaatatg ctttatatga ccgtgggtgt ttttcggcag agacttgctt
     7681 taagtaaggc agccaagatt ctatttgcct gtattttttt caatatgatt ggcggtatat
     7741 tatgtggatt tctaatttct atgactacgc catttcaaga tttaacgttg gacaacttta
     7801 tgttagaatc aatagcagta aaactagcaa aaccttcagc aacgattttg gtggaaggga
     7861 tgtttgcgaa tatcgttgtc aataccgctg tactgatcag catgcggatg aaagaggatg
     7921 tcggaaaagt gatcacagtc gtattcatca ttttcatttt tgctttttta ggatatgaac
     7981 acgtgatagc gaattttcct gcctttacgc ttacttactt tgcttctcac ggacagatgg
     8041 atggaatgac agtcagcaat gtgttacata atttgttttt tgctttggca ggaaattata
     8101 ttggtggcgg tttagtaatg ggactgggat atgcttggct gaatcagtca aaatcatcct
     8161 atgtggacta aaaagtcgtt cattaaaggg gaagtgccat ggaagtttta gtttttccaa
     8221 aaaatgagga aaaagcgtta caaatggaaa agtacatgaa aaatcttttt tcctttgctg
     8281 gagtacctaa gccggaacgc gctcttttac aacaaccgtt actggatgag agcaaaaatt
     8341 tatctatttc tgaagtttta gcttttgttc attattatta tgaacaaatc gaaagagagt
     8401 atcagtatgt agcgatcgat ttagtccaga aaaacatcaa acggcttaca tttgaagact
     8461 tagtcgtatt gttgccacta gttcagaaaa aagaatggtg ggacagcatc gactcttgga
     8521 gaaaagtata cagcacatgg tgtaaagcct acccagagca gcgtgaagaa gtctttcaat
     8581 tgtttgaaaa aaaggaagat ttttggctac gtcgaatagc aatcacgttg caattaggtt
     8641 ataaacagca aacagacaca ggactattga tccgagcaat agacgaagat cgtcaaacaa
     8701 aggaattctt tatccaaaaa gcaattggtt gggcattacg cgattatagt aagaccgatg
     8761 ccgattgggt aaaaagacag ctgacaacag atttgagtaa gttagctgtt cgagaggcaa
     8821 gtaagtattt ataagaaagc gagacgacaa gagatgagac gcaaattatt tgcttttgat
     8881 attgatggaa cgttgttaga ttcaaacaaa caagctttag atagcacaag agaagcattg
     8941 gaaaaattac gcaaagccgg tcatctggtc acgattgcta cagggcggag ccgatttcat
     9001 gcgcaagaga tcattcgcga tcttgatttt acgaactaca ttttatgtaa tggagcggcc
     9061 ggttttttag accatgaaca agtatataag aatttactag atcgcgacca gttggatgcg
     9121 ttcgttcaag aagcgcatca tgagcagatc gatacagctt ttgtcagctt agacaatatc
     9181 aagcgttctt cttccaatga tgtaacggtg atggaagaag cgatgcattc tttcggtgct
     9241 gtcttgcctg aattagatca gttttttgca gaggaacaag aaatatacca agcgttagca
     9301 tttttcgatc aaacatatga tgggaagttc tcttcatatg ataaaattcg atttgtccgc
     9361 tggcatgaga acagtgtaga tgtcgtacct aaaaatggtt ctaaagcagc gacaattctg
     9421 cacttagcag aacaagtagg catccctcat gaagacatca tcagctttgg cgatggacaa
     9481 aacgaccgtg aaatgttgcg catgtcagga attgggatag ctatggggaa tgctgtgcca
     9541 gaagtacaag cagaagctaa gatggtcact gatacaaacg atcaggatgg aatttggaag
     9601 gcattgaaag agttaagcgt gatttaaaag aggttgtgaa aaagctgttc tgcatcaagt
     9661 aataagccca gccaaacaaa aatagctcct cctatttttc gtttttctgg gcttattacc
     9721 gcagatgcaa gctttcgaac accgtttaag gaagaggtcg tgacagaagt ggtcagtttc
     9781 aagaaataag aagggattta cgaaaattgc ttctcaaatt tttgtaaaat tacggcttat
     9841 ttctgaagaa gtagatgaaa caacgtttat tcgggaaatg ggactgtgac aagatgtttg
     9901 tcatagtccc atttttttat tttatttttc aaaactaaga aataccagga aagagttcct
     9961 tttgtttgac cttttctttc ttattgaaaa caactataat atagagtgat gttaaaagag
    10021 aagaggaatt tagtgtgcgg gtattgttta ttggtgatgt agtaggttca atgggacgag
    10081 agatgattac tgaatatctt cctagattga agaagaagta ccgtccgcaa gtaacgattg
    10141 taaatggtga gaatgccgct tctggacgtg gaatcacaga aaaaatctat aaaaaatttt
    10201 tgcaagacgg cgtagatgtc gtgacgatgg ggaatcatac gtgggacaac cgcggaatct
    10261 ttgagtttgt aaatgaagct aaaaagatgg ttcgtccggc aaattttccg gaagggacgc
    10321 ctggacaagg gatggtcttt gtcaaagtga accaagtcga actggcagtg atcaacatgc
    10381 aggctcgttc ttttatggtg gacttggatg atccttttcg taaaatgaat gagctggtag
    10441 aagaagccag aaaacgtacg ccgatcattt ttgtagattt tcatggagaa acaacaagtg
    10501 aaaaacaagc aatgggctgg tttttagatg ggaaagtttc agcagtggtc ggtacacata
    10561 cacacgttca gacaaatgat gcgcgaattt tgccaagagg aacagcgtat ctgacagacg
    10621 ttgggatgac aggtccctat gatggtattt tgggtatgcg cagagaacct gtaatcgaaa
    10681 agtttctgac tgcattacca aaacgttttg aagttgtgga acaaggccga tcgattttaa
    10741 gcggctgtat cttggaattg gatgatacga caggtcatgc aaaagaaatc cagctgattc
    10801 agatcaacga tgatcgaccg ttcatggagt agaaagagag gcagtttttt gcagcaggag
    10861 ccggaaattc aaaaagaagt aaggaaactg acaaaacttt taagagagaa tgaaacgatc
    10921 attcgctaca aagaacttga agaaaagatc cagcaaaacc agtatttagc tgagttgaga
    10981 gaaaaaatca aacaagcgca aaaagatgct gttcattttg cccactatga caaaccagca
    11041 gcagaaaaag aagcaatcaa acaagcggat caattcatgc aagaatttga tcagcatccg
    11101 cttgttgtag cttatagaaa acagctgttg gaagcagatg atctgcttca tcatttaaca
    11161 actatgatac aagaagaaat caatggacaa atagaggagg aaaaacatgc ctcaaaaaac
    11221 taaaaatact cctatgatgg aacaatatct aagcatcaag gcccaatacc aagatgcttt
    11281 tttgttctat cgtttaggag acttttatga actattttac gaagatgcga tcaaagcgtc
    11341 acagcttttg gaattaacgt tgacgagcag aaatcgaaat gcagaagaac ctattcctat
    11401 gtgcggtgta cctcaccatg cagcacaagg atatatcgat gccttgattg aaaaaggcta
    11461 taaagtagcg atttgcgagc aggtagaaga tccaaagacg actaaaggga tggtcaagcg
    11521 cgaagtgatc cagctagtca ctcctggtac agtcatggac agcaaaggat tgtcggcaaa
    11581 agacaacaat tttttaacag ctgtagttag tcaaggaaat gaatttggct ttgcttatgc
    11641 cgatttaagt acgggagaat tgaagacggc tcggttgcat gatgaagaag ctgttttaaa
    11701 tgaagcctct gcattgcaga caaaagaact cgtattaggc agtgagatca ctgataccct
    11761 tcgtgaacag ctaagcgggc gtttagggct agtcttttct caacaaaatg agatggaaga
    11821 aaacgcggaa tttagctttt tgacaagcga actagtcgat ccgctggaaa aagaagcaac
    11881 cggcaaacta ttgacttatt tatcggtgac acaaaagcgg agtttagctc atatccaaaa
    11941 agcagtagaa taccaaccag atcatttttt gaaaatggat tattattcaa aattcaacct
    12001 agaattaagc caatctattc ggactgggaa aaagcacggc actcttctat ggttgttaga
    12061 tgaaacaaaa acagcaatgg gtggccgttt actaaaacaa tggctagacc gtccgcttat
    12121 ccaggcaaaa caaattaaag caagacaaga aatggtcgct tctttgctag acagctattt
    12181 tgaacgtatc gatttgcagt cagcattaac gaaagtctat gatctggaac gcctagcagg
    12241 tcgagtagct ttcggtaatg tcaatgggcg agacttgatc cagctgaaaa cttctttaga
    12301 acaagtgcca atgatcagag ggttgattga aggaatcgat caaggtcaat ggcaaagtct
    12361 gctttcagaa atgcagccga tggaccatct cgtccaattg atcgaccaag ctatccaaga
    12421 tgatccgccg ttacaaataa cggaaggaaa catcatcaaa gatggattca atgagcagct
    12481 cgatatgtac cgttctgcta tgagaaacgg aaagaaatgg ttggcagaac ttgaagcaaa
    12541 agagcgtcaa gaaacaggaa tcaagaactt aaaaatcgga tttaaccgcg ttttcggtta
    12601 ttatatagaa attacaaaag caaatcttgg aaatgcagaa ctggagaagt atgaacgaaa
    12661 acaaacttta gcaaatgcag agcgtttcat cacaccagaa ctaaaagaat tggagacaca
    12721 aatcctcgaa gcagaagaaa aatctgttga tttagaatat cagttgtttt tagctgtgag
    12781 agaagaagtc aaaaaggcta tccagccgct acaagtactt gcaaaagcaa tcagtgcagt
    12841 agatgtgcta caaagttttg caacgatcag cgaacgctat cagtatgttc ggccagaact
    12901 tgtcagtgac aagcatcagc ttctgatcaa agacggacga catcctgtag tagaaaaagt
    12961 attaggacat caggaatata tccctaacag cgtagaaatg gcagaagacg aaatgatctt
    13021 gctgattaca ggaccgaata tgtctggtaa aagtacgtat atgcgacagt tggcattaac
    13081 tgttctgatg gcgcaaatgg gctgtttcgt tccggcaaaa caagcagttt tacctatttt
    13141 tgatcggatt tttacccgaa ttggtgcaag tgacgactta attgctggac aaagtacttt
    13201 tatggtagag atgatggaag ccaatcaagc cctgcgttat gctacgccaa acagtttgat
    13261 tttgtttgac gaactagggc gaggaacagc aacctatgac ggaatggcgt tggcccaagc
    13321 aatcatcgaa tatatccatc gtcatgtaca agcaaaaaca ctcttttcta ctcattatca
    13381 tgaactgacg gtacttgaga aagaacttcc gcagcttaaa aatgtccatg ttggagccgt
    13441 tgaaaaagac ggagaagttg tcttcttgca taaaatgatg gatggacctg cggataaaag
    13501 ctacggcata catgtggcaa agatcgcagg attgccttcc aatctactag aacgagcagc
    13561 agatatcctg catacgctag aaaatggcga taacggacac catactgttc cagctgaagt
    13621 agaagagaaa actaaaacag ccaaacagga gcaaataaaa ccacctgcag aaaaagtaaa
    13681 tcaagaccat atacaagacc gtgtaaaaga agaaaaccaa cagctttctt tattcagtga
    13741 attatccgaa aatgaaacag aagtcattga atcactgaag caaataaatt tactagaaat
    13801 gacgccaatg gatgcactaa acacattata tgaattacaa aaacaactgt aggaagaagg
    13861 cggatatcga tggccaaaat ccaagaatta tctgaacgtt tagctaatca gatcgctgcg
    13921 ggagaagtag tggagcgacc agcttccgtt gtgaaagaat tagtggaaaa tgcaattgac
    13981 gccggcagta ctcagattga tatcttagtt gaagaagcgg gactgcggac gatccaagtg
    14041 attgataacg gagaaggcat tttggcagac gacgtagaga atgccttcaa gcggcatgcg
    14101 acaagtaaga tccataatcg cgacgatctt tttcgcatac gaacgctggg ctttcgaggg
    14161 gaagctttgc caagtattgc ctctgtttct gaaatgactc tggaaacggc gaatcaggaa
    14221 gaaaaacaag gcacctatct gtcgctgaaa ggcggagaag ttattgagca tcgaccagca
    14281 ccactgcgtc aaggaacgaa gattaccgtc agcaatctgt ttttcaatac gccagcccgc
    14341 ttgaaatacg tgaaaacact gcacacagaa ttagcgaaca tcggggacat cgtcaatcga
    14401 ctggcattga gtcatccatc gattgctttt cgtttagtac atgatggaaa caaaatgctg
    14461 gcaactgctg gaaatggcga tctgaaacaa acaattgcgg gaatctacgg attatctacc
    14521 gcaaaaaaaa tgctgaaaat cgaaggaaaa gatctagatt ttgagttatt tggctatgtt
    14581 tcattgcctg aggtgactcg tgccagtagg aattacttat cgacgatcat caatggacgt
    14641 tttatcaaga acttttcctt gaataaagca atcgttgcag gttatgggtc aaaattaatg
    14701 gtcggtcgtt tccctattgc tgtattggaa atcaaaatgg acccgctttt agtagatgta
    14761 aatgttcatc cgacgaagca ggaagtccgg ctgtcgaagg aaaaagaact aacagagcta
    14821 attagtaacg ccattcaaaa agcattgtct caagaaaatc tgattccaag tgcagcggat
    14881 aatctacggt ttaagaaaaa agtacctgat cagcctaaaa cagaacaaat cgaaatcgat
    14941 ttgtcctacg cttttgaaga agaaaaacct aaaaagccaa gtagtttgaa ttttgatcga
    15001 gagacaggta cctttttcgt agaagaacta aagaacacat ttgttgacag aattgttgat
    15061 aagtctgtgg aaaatgttga aaactattct ggtttttctg aagaaaacac cacttatcca
    15121 gaaaaaacag cgaaaacggt ggataagttt ggtgaaaatg ttgataactt tgtagaaaaa
    15181 acagtggaaa aatctgaaaa gaaagaattt cccattgtgg ataacatgga gaaaacaaat
    15241 gaagaacaag cagataaatc cgtagaagag gatgaaaaga tttacgagga agaattgaca
    15301 ctccatcctg aatttgattt cactcaagga aacgacaaaa cattagaaca agccatcaag
    15361 aaattagaaa cggaaaaacc agtacaacgt tttcctcagt tggaatattt tggacagatg
    15421 cacggaacgt atctttttgc ccaaagtagc gatgggttgt acatcattga tcagcatgct
    15481 gcccaagagc gcattaagta tgaatatttc cgggaaaaaa ttggggaagt atctgatgat
    15541 ctgcaagaat tgcttgttcc atttgtgtta gactatccaa acagtgatgc tttgaaatta
    15601 aaagagcaaa aacacttgct agaagatgca gggatttatt tagaagaatt tggtcaaaac
    15661 agctttattg tacgggccca tcctacttgg tatcccaatg gagaagaaga atcgattatt
    15721 cgggagatga tcgatatgct gctgacaact ggaagtgtga gtgtaaaaaa attccgtgaa
    15781 gcaacagcaa tcatgatgag ttgcaaacga tcgattaaag ccaaccatta tttgaatgaa
    15841 gcacaagcac gtgtgctgct gaaggatctg gcacgatgcg aaaatccttt caattgtccc
    15901 catggccgtc cagtattgat tcactttacc aattcggata tggaaaggat gttcaaacga
    15961 atccaagacc cgcattaagt caaagagaag agggaaaaca tgaagattgt attagcatcc
    16021 caatcaccaa gaagaaaaga gttattggct catatcgttt cagaattcga gattgaacct
    16081 gctgatatcg atgaaacgcc atttccagac gaacagcccg tcgattatgt tcgtagaatg
    16141 gctgaagaaa aagcacgaac tgtctgggaa caagatgaaa aagaagacca tttagtgatt
    16201 gccagtgaca cgactgttgt tctcaatcaa gagatcatgg gtaaaccaga agatctggct
    16261 gaggctgaat tcatgctgaa aaaactaagc ggggaaacac atactgtata tacagctgtt
    16321 gttttaaaaa caaaggaacg agaagaacgg atactagcag aagcacatgt cactttttat
    16381 cctttgacag acgaagagat caaacgctac ttggagacgg gggattatgc ggataaggca
    16441 ggggcatacg gcatccaaaa tgctgcgagt gtattcgtca aagaaatttc tggagattat
    16501 tacagtatcg taggatttcc gattggtgct gtcaatcagg cactgaaaac attcatttaa
    16561 acaaaaaagc ggactatctg attttcgatt gaaaatccat gatagcccgt tttttttatt
    16621 gaaaagatta cttatctact ctgactaaga ttttacttca gctttaccga ctgcttcgcc
    16681 cgggcgaaca agtcctgatt ggtcaagaga aagtcctgca actttttcat tattggtcaa
    16741 agcaacaata atgtcagttt tcttgccagc ttgttctatt tctggcaaat ccatttcagc
    16801 aatcagggtc tctggtgtga caaggtatcc ttctttcacg aatacattga aaggaacacc
    16861 ctttagttca acagtatcta atcccatgtg aacaagtact tccagcccct cattagaaag
    16921 aataccaatg gcatgttttg tttcaaagac actcgtcact ttaccgttta ctggtgcata
    16981 gactttgccg tttgacggtt cgacagcata accgtcaccc atcatttttt gtgagaaaac
    17041 tgggtcattt acttcagtta tttccttgat ggtccctgca gcgactgcga ataatgtttc
    17101 ttttgttgca actgccgtac cagttgctga aacaggttcg aaagactgtg gcgttgctgt
    17161 tgcgccattt gctgtttgaa gttgtacatc agcagcattt tcaggaacag caggatcaat
    17221 cttattcaaa attccctgac gtctaaagat gatcgtcaag ccaaatggaa tgatgacagc
    17281 aatgatcatt gcgatgatga aaggtaccca tgtttctgct tggatagcta gtattcctgg
    17341 caaaccgcct actccgatcg cgttagctcg aacacccatc aagttagcaa acatgccagc
    17401 taatccagag ccgaccattg cagcgacgaa aggatagacg tatttcaaat tgattccgaa
    17461 catagcaggt tcagttacgc ctaagtaaca agaaatcatt gctggaatcg agatttgttc
    17521 ttctttttta ttcccacgat gtaagaagat gatggctaag actgcagagc cttgggcgat
    17581 atttgataaa gcaatcatcg gccataaatt cgttgatccg aaatctgcaa tcagctgcat
    17641 atcgatggca ttggtcatat ggtggagacc agtaatcact aaaggagcat acaggaatcc
    17701 gaatacggca ctaaacaacc agctgataga agacgtcaat ccagtattta cgatatttga
    17761 gatccagctt ccgactgtcc agccgatagg acctaaaata acgtgtgcag ctaaaacagt
    17821 tggtaacaat gaaaacaatg gaacaaaaat catcgagatc gactgaggga tatatttgcg
    17881 gaagaagatt tctagatacg ctaacataaa ccctgccagc atggctggaa taacttgcgc
    17941 ttgataacca atcatctgga cttgagcaaa accaaaatcc caaaatggaa tatctgctgc
    18001 ggctgtggaa gctacactat aagcatttaa taattgcgga gaaactaacg taattcctaa
    18061 aacgattcct aatatttgtg tagtacccat cttttttgca atactccaag tgatacctac
    18121 tggaagaaaa tggaaaattg cttcacccgg taaccacaaa aaggcattga caccattcca
    18181 aaacttcgag tgttcaacga ttgttccacc aagactttca aactggatac cttcaagtac
    18241 atttctgaat ccaaggatca atccaccgac aatgattgca ggaatcagtg gtgtaaagat
    18301 ttctgctaaa acggcaatag cacgttgaac gggatgaagg ttttgttttg cagctgcttt
    18361 tccttgttct ttggaaaccc cttcaactcc agatactgca acgaagtcgt tataaaaagt
    18421 agaaacatcg tttcctatga tgacttggaa ctgtcctgca ttcgtgaaca tcccttttac
    18481 gctaggtata tcttcgatcg ctttttcgtc cgcttttcct ggatcattca ggacaaaacg
    18541 catacgagtc gcacaatgac taactgcagc aatattttct tttccgccga tatcatgtaa
    18601 cagcttctcg gcatctgctt gatattttgc catatcaata aacccctttc tcttaacttg
    18661 tctatgcaag ttgtttcttc atttactata ctttttttcg gagagaattg caagcgtttt
    18721 attaaaaagt acgagagaaa atgatatcgt tttaaaaagc ttaaaataag cttttcttaa
    18781 aaataggaac attgaaatac aaaaagagct gtagtatagt gaagaaatga tggaagaacg
    18841 agtgagggaa aaaacatgaa taagttcaat gaaatttttt tagatttgga acagaaaatt
    18901 ttagcgaaag aatatcctcc gcattcgctt ttacccagtg agaaccaatt gatcaaaatt
    18961 tatggtgttt cacgagaaac aattagaaaa gcgctgaatc ttttgactac ggaaggttat
    19021 attcaaaaaa aacaaggaaa aggttcgatc gtactggatc ggaatcgctt cgactttcct
    19081 atttctggat tgactagcta taaagagcta caggagaccc aacgtattcc aagtgaaacg
    19141 atcatccata cgttagaaga aacagaagtg acaaaagcat tgaacgagat cactggctgg
    19201 gagatcggtg cgcctgtttg gcatttgata cgcgaaagaa aaatcaatgg cgaagtagtt
    19261 atattagaca ccgattatct attaaaggaa atcgttccta ttttaactcc agcacaagca
    19321 caaggatcga tctatgaata ttttgaaaat gagctttctt tgacgattgc ttacgcgcaa
    19381 aaggagatca cagtagaaga agtaacggaa atcatgaaaa cagcgatgga tcttcacgat
    19441 gacacttatg cagtagtagt cagaagcctc gttcatctgg aagatacccg ctgtttcgag
    19501 tattctgagt cggttcaccg attagataaa ttccgtttcg ttgagtttgc ccgacgccga
    19561 aaagtgtaaa tagttcaaaa gaaagcaaat tgttggcatc tgtccacttt ggcgctatga
    19621 taggatcaca aagggggcat ttttaatgga taaaccaata agaccagaaa aagaagaact
    19681 gaaagaaaag ttatcaccca tggcttatgc agtcacacaa gaaaatgcaa cagaacgacc
    19741 atttagcggg aaatatgatg atttttacga aaaggggatc tatgtagata tcgtcagcgg
    19801 tgaaccgtta ttcagctctg cagaaaaata tgatgcaggt tgtggctggc catcattcag
    19861 caaaccaata acgaaaagac aagtgcgcga aaaagccgat ttttctcatg gcatgcatcg
    19921 aatagaagtt cgaagcaaag aagcagattc tcatctcgga catgtgttta ccgatggacc
    19981 agtagatcaa ggtggtctac gttactgcat caattctgcc tcactgaaat tcattccata
    20041 tgaaaagatg gatgaattag gttatgggga attcaaatca cttgtagaat aatggaaata
    20101 cacaaagagc ggtacaatac ggggatttac tcgatcgtcc gctctttttt tattcaagcg
    20161 attgctattt gaaagaaacg ctccctagac tccttttttc acaaggtgaa atgtgctaca
    20221 ataaagggaa cgtatgtgtg caaaagaaag aggactagaa tatgtatgaa tatctgacag
    20281 gtgtggttac ctttatcaac ccctattaca tagtagtaga aaccaatggt atcggctacc
    20341 agatcgcact aggaaatcct taccgctaca gtagcaaact aaatcaaaaa ataaaaattt
    20401 atgtccatca ggtaatacgt gaagatgcgc atcttctgta tggatttgat tcgctagaag
    20461 aaaagcaact gtttttgcga ttagtaagtg tttcaggaat cggtccgaaa agtgcccttg
    20521 caatcatggc aagcgaagac cactccggtt tgattcaagc agtagaatcc agtgatgtca
    20581 cttatttgac gaaatttcca ggagtaggga aaaagacagc acaacaaatg atcttggatc
    20641 taaaaggaaa attcggtgag ctgagtatcg atacaccttt tagtctattt gatgaagcaa
    20701 acacaaaaga cgctacagct ctatctgaag caatagaagc tctctcggct cttggttaca
    20761 gtgatcgaga aatcaaacgt gtagaaaaac aattaaaaga agtagataat cagacaacgg
    20821 atgagtatct acgccaagca ctcaagctaa tgatgaaaaa ataaggagga gaccaaatga
    20881 cagatgaata cttgttatca ccagaatctg gcgaaaatga actgagtttg gaaaaatctc
    20941 ttcgaccaca gctgctttct caatatatcg gacagagaaa agtcaaacaa gaacttcaga
    21001 tttacatcga agctgccaga aatcgggaag aacctttaga tcatgttctg ttgtacgggc
    21061 ctcctggatt aggaaaaacg acgatggcca tggtcattgc taatgagatg accgtaaata
    21121 ttcgtacgac gagtggccct gctatcgaac gtccgggtga cttagtagca atcttgaatg
    21181 aactggaagc aggggatgtc ctgtttatcg atgagatcca tcgattacct agagtggtag
    21241 aagagatgct ttattcagct atggaagatt tctatgtaga tatcatggta ggacaagggc
    21301 caaccgcaca tccggttcat ttccctcttc ctccctttac tttgattggc gcgacgacac
    21361 gggcaggtat gctttctgca cctttacgag atcgatttgg catcatttct catatggaat
    21421 attatgacga acaggatctg caggagatcg ttttacgttc ttcggatatt tttcaaacag
    21481 agatcattga agaaggggca ttggaaattg ctcgccgatc acgaggaacg cctagaatcg
    21541 ctaatcgctt gctcaaacgg gttcgtgatt ttgcgcaagt ccaaggagac gggaaaatcg
    21601 atcgtcctat agcggatcag gcacttactt tgctacaggt cgatcaggct ggattggatt
    21661 atgttgacca gaaattgttg cgaacaatga tcgagttata tggcggcgga ccagtagggt
    21721 taagtacgct ttcagtaaac ataggagaag agcgtgagac ggtagaagat atgtatgaac
    21781 cgtatctgat ccaaaaagga tttttaaagc gtacacctag agggcgaatt gctactgctt
    21841 atgcctatga acattttggt tacgactatc atatatgatt tttgctttta gattggagga
    21901 aaatgaatga gtgatacaac acgtacgaaa caattgatca aaaacgtatt gattgatttg
    21961 tgccaaacgc aatcttttcg aaaaatcagc gtccaaaaaa tcgcaaacga agcaaaaatc
    22021 aatcgacaaa cattttatta tcattttaca gataaatacg atttgcttcg ttgggtctat
    22081 tgtgaggatg cattgcatta tttagatcca caatgtcttt cacttgataa ttgggaagag
    22141 caagcattga aaatgctgaa agcaattgct gagaagaaag aattttatgc aaatactgtg
    22201 ctttctgacc cagaaatttt acaaaaagag ttttctcaac tgatccaaaa atcatttatc
    22261 aagatttttg aacaaatgga tgaagaaaga caactttctg caagagacaa actattttat
    22321 gctcgttttt tttcttacgg ttgcagtggt gtattagtca attggatcac gaatgaatat
    22381 gaagaaacac cgatggaaat cgctatgcag cttttccgtt tagccaagga tacggaatta
    22441 tattcttatc gtctttatgc aaaagaagag ttggaagaac agtaatagaa aaattttttg
    22501 ttggaaaagc aataatcgct aaaaggagat ggggaaatga caaaaatttt atttgtttgt
    22561 ttgggaaata tttgccgttc accgatggca gaaggcttgc tgagaaaacg aatagcatta
    22621 gaaggaaggg aagcagaatt ttttgtggat tctgctgcta caagcacgta tgaagttgga
    22681 aagacaccgc accctggaac gaaaaaaata ttaaaccaag aaaaagtaga tatgacgaat
    22741 atggttgctc ggcaaatcac accccacgat tttgaaactt ttgattggat cattggcatg
    22801 gatcaagaaa atgtagaaga actgaaacga agagcaccac gctcagcgca ggggaaaatc
    22861 catttgtttt taagtagcat tcctggaaaa gaaaaagaaa atgttcccga cccatactat
    22921 accaataact ttgatgagac atatcacttg atcacagaag gcctgaatca ttggtggaat
    22981 atttggattg atgaaaataa aataacaaat aatttgtaaa gttaacgctt tctttttaag
    23041 gcgatgttag agcatttcct tgtttttttg taaaatgtta ggtaaaataa aattaattaa
    23101 gtaacacttt cttagctaaa ggaggagtgc attcgatgaa cgaagagaca gtatcgccaa
    23161 tgaagaagct tacgaagtta gttactcata cttcgtatca actttttgat tcttttgtaa
    23221 atgacactga agaacatgct aagaatgtac gattgcaagg aaagtttatt cttgagctgc
    23281 aaaaagctgc tactgaacat agtctagtta tcttacaagt aagagcaaat cgtttgagta
    23341 gcaagtatga aacgatctca ggctgggtgg cgacaaaaac ggtcgaaaaa gaccgagtga
    23401 tgatccgcct acaaaacgaa gagcagcaga tccggatggt tcctgtagaa caaattgaaa
    23461 aaataacaat cctttcacca gcaggggaac aggaacggtt atcaaaataa tttataccag
    23521 atagaaaagc acgtatgagc ttgtaccatc aatcattact aatcaaaaat aaccgaatgc
    23581 tttaagctga tatcagtaaa acggaacgtt ttaggatgag tatcatagca tgacagcatt
    23641 cggttgat
//
LOCUS       DACDXO010000041        23075 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E23605_2017
            SAMN12639989-rid9881463.denovo.041, whole genome shotgun sequence.
ACCESSION   DACDXO010000041 DACDXO010000000
VERSION     DACDXO010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639989
            Sequence Read Archive: SRR10023389
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23075)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23075)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741914.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:42:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,972
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 33 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 17 of 73
            Pseudo Genes (multiple problems)  :: 10 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23075
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639989-rid9881463.denovo.041"
                     /isolate="E23605_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            101..2542
                     /locus_tag="IWM35_001786"
     CDS             101..2542
                     /locus_tag="IWM35_001786"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HAP9919635.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
     gene            complement(2872..3576)
                     /locus_tag="IWM35_001787"
     CDS             complement(2872..3576)
                     /locus_tag="IWM35_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9919636.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
     gene            complement(3763..4008)
                     /locus_tag="IWM35_001788"
     CDS             complement(3763..4008)
                     /locus_tag="IWM35_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9919637.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
     gene            complement(4025..4876)
                     /locus_tag="IWM35_001789"
     CDS             complement(4025..4876)
                     /locus_tag="IWM35_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HAP9919638.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            5042..6046
                     /locus_tag="IWM35_001790"
     CDS             5042..6046
                     /locus_tag="IWM35_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HAP9919639.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
     gene            complement(6287..6643)
                     /locus_tag="IWM35_001791"
     CDS             complement(6287..6643)
                     /locus_tag="IWM35_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAP9919640.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
     gene            7011..8198
                     /gene="tuf"
                     /locus_tag="IWM35_001792"
     CDS             7011..8198
                     /gene="tuf"
                     /locus_tag="IWM35_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAP9919641.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            complement(8386..10977)
                     /gene="mprF"
                     /locus_tag="IWM35_001793"
     CDS             complement(8386..10977)
                     /gene="mprF"
                     /locus_tag="IWM35_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HAP9919642.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(11003..11722)
                     /locus_tag="IWM35_001794"
     CDS             complement(11003..11722)
                     /locus_tag="IWM35_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP9919643.1"
                     /translation="MKKEKKYAKMPDGSEIYYEKSGQGFPLFLLHGNDGSGRFFSEQV
                     PVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEKIDQADFLGFSDGAN
                     LALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVWLLSLFRPSLRKNLL
                     VIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKTIPKASFVLVKEQGH
                     ELARKDPERFNREVLQFLSET"
     gene            12042..12563
                     /locus_tag="IWM35_001795"
     CDS             12042..12563
                     /locus_tag="IWM35_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HAP9919644.1"
                     /translation="MESYQIEITTAAKNYHMENYENLIKAIILTESKGKGTDLMQSSE
                     SLHGEAEIIDNPADSIDQGVKYLAEMIVEAKKQGCDLWTAVQAYNFGKDYIAYVKNHG
                     GENTVELAEAYSKDVLSPLLGNEEGKQYRYWRIQSLLYNGGYLYHNGGNMFYPDIVKM
                     NEKIIETYEKVFK"
     gene            12622..13482
                     /locus_tag="IWM35_001796"
     CDS             12622..13482
                     /locus_tag="IWM35_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HAP9919645.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            13589..15982
                     /locus_tag="IWM35_001797"
     CDS             13589..15982
                     /locus_tag="IWM35_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAP9919646.1"
                     /translation="MFLILEHARLKHVIIFESWRKMEKKKMNGLTQQEVVQKMASGQQ
                     NDYQEDASKSTKQIFSDNILTLFNFLNFGIGICLLFVGAYSNMAYLAIIVVNIVIGIY
                     QEIHARNLVRKLSIVSQSEVLVIRDGKEQSISTKELVLGDLVKIGAGEQIPADLQVIS
                     GRAEANEALLTGESDLIVKETGAELLSGSFLTSGSVMAEVIRVGADNYAVRLTSEAKT
                     HKAIRSELVDSIRKVSKFTSFVIIPLGIILFLEALFIRDASIKISVIASAAALLGMLP
                     KGLVLLISIALTTGVTKLAKKRILVQDMYSIETLAHVDTLCLDKTGTITEGKMSIQRV
                     EALHPLYEKAIPEIMGTYLAESSDNNITMRAMRDYFELSNRYDVTNKVAFSSDRKWGA
                     MEFPELGVVYVGAPERLVEEDRLPDSLKTAQANGYRVLMLGIAPNQQLNEEKPAFVEP
                     VALFEIDDPIRKNAQETLAYLHEEGVDLKVISGDNPFTVSNIARRAGLPNYDAYVDLS
                     QITEEMEVREAAHRYTVFGRVSPQQKKLLVNELKESGRTVAMTGDGVNDVLALREADC
                     SIAMAEGDGATRQIANLVLLDSDFTTLPEVLFEGRRVVNNVTKVSGIFFIKTIYSFIL
                     SIICAVTAIGFPFIPIQITLIDLAIEGYPSFFLSFEQDKRKITYRYLPTALINALPNA
                     LLVVVNIIAVYLLGKTFAWGNAETTTLMYYLLIGISCMAVVKACYPFNPLRIFLAVTT
                     IVGIYVAAMLFHGILEVNLGLGESVGYFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            16164..18005
                     /locus_tag="IWM35_001798"
     CDS             16164..18005
                     /locus_tag="IWM35_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HAP9919647.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVQNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            18029..19138
                     /gene="rpoD"
                     /locus_tag="IWM35_001799"
     CDS             18029..19138
                     /gene="rpoD"
                     /locus_tag="IWM35_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HAP9919648.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
     gene            19358..21457
                     /locus_tag="IWM35_001800"
     CDS             19358..21457
                     /locus_tag="IWM35_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HAP9919649.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
     gene            21653..22516
                     /locus_tag="IWM35_001801"
     CDS             21653..22516
                     /locus_tag="IWM35_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAP9919650.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            22621..>23075
                     /locus_tag="IWM35_001802"
     CDS             22621..>23075
                     /locus_tag="IWM35_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAP9919651.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKE"
BASE COUNT         7770 a         4038 c         4810 g         6457 t
ORIGIN      
        1 ttaaggattg ttaaaattta atcttagaaa aaaataactg gaacaagagc tatttgtttt
       61 tacagcaaag aatggtatat ttttgtagga ggtggcttac ttgaaagaag tcgttatgat
      121 agatgctgca agaacgccga taggaaagta tcgcggaagt ttaagtccat ttactgcagt
      181 tgaattaggc acacttgtga ctaaaggatt attagataaa acaaaactga aaaaagataa
      241 aatcgatcaa gtaatatttg gaaatgtcct gcaagctggt aatggtcaaa atgttgccag
      301 acagattgct ttgaacagcg gtctccctgt agatgtccca gcaatgacga tcaatgaagt
      361 ttgcggatct ggtatgaaag cagtgatctt agcacgccag ctgatccaat taggtgaagc
      421 agaacttgtg atagccggcg gaacagagag tatgtctcaa gcacctatgc tgaaacagta
      481 tcagtcagaa acaaatgaat atggtgaacc aatttccagt atggtcaacg acggattgac
      541 tgacgcattt tcaaatgcac atatgggatt aaccgcagag aatgttgcaa cacaattttc
      601 tgtgagcaga gaagagcagg atcgctatgc cttgtcgtcc cagttgaaag cagcacatgc
      661 tgtcgaagcc ggtgtatttt ctgaggagat catcccagtc aagatttctg atgaagacgt
      721 gttatctgag gatgaagcag ttcgtggaaa tagtacattg gaaaaactgg gcacgttacg
      781 tacagtattc tcagaagaag gaactgtaac agcaggaaat gcttccccgt tgaatgacgg
      841 tgcctctgtg gtgatccttg catccaaaga atacgcagaa aataataatc tgccttattt
      901 agcaaccatc aaagaagtag cggaggtcgg tattgaccca agtatcatgg gaatcgctcc
      961 aatcaaagcc attcaaaaat tgacagatag atcaggaatg aatttatcaa caatcgatct
     1021 ttttgagatc aatgaagcat ttgcagcttc ttctatcgta gttagccaag aactgcagtt
     1081 agatgaagaa aaggtcaata tatatggagg agcaatcgca ttaggccatc caattggtgc
     1141 aagcggtgca cgtatcttga ctacattagc ttacggattg cttcgtgaac agaagagata
     1201 cggtattgct tcattatgta ttggtggagg tttaggcttg gcagtcttgt tagaagcgaa
     1261 tatggagcaa acacataagg atgtgcaaaa aaaaaagttt tatcagctta cgccatctga
     1321 acggcgcagt caattaatag aaaaaaatgt attgacacaa gaaactgcgt tgatttttca
     1381 ggaacaaaca ctttctgaag agctctccga tcacatgatc gaaaaccaag tgagtgaagt
     1441 ggaaatccca atggggatcg cgcagaattt tcaaatcaat ggcaagaaaa aatggattcc
     1501 gatggcaaca gaggaaccat ctgtcattgc agctgcaagt aatggtgcaa aaatctgtgg
     1561 aaatatctgc gcagaaacac ctcagcggct gatgcgaggg caaatcgttc tatcaggaaa
     1621 atcggagtat caagcagtga tcaatgcagt taatcatcga aaagaagagc tgattctttg
     1681 tgcaaatgaa agttatcctt caatcgtgaa acgcgggggc ggtgttcaag atatctctac
     1741 tcgggaattc atggggagtt tccatgcata cttatctatt gattttttag tggatgtaaa
     1801 agatgcaatg ggcgctaaca tgatcaactc gattcttgag agtgtggcaa acaagttacg
     1861 ggagtggttc cctgaagaag agattctatt cagtatttta agtaattttg ctacagaatc
     1921 attggcttct gcctgttgtg aaatcccatt cgaacgctta gggagaaaca aagaaatcgg
     1981 tgaacaaatt gccaaaaaaa tccaacaggc aggggaatat gcgaagcttg acccttaccg
     2041 tgcagcaacg cacaacaaag ggatcatgaa tggtatagaa gctgtagtgg cagctacagg
     2101 aaatgataca cgtgcggtta gcgcttcgat ccacgcctac gcggcaagaa atggattata
     2161 ccaaggatta acagactggc agatcaaagg ggataaacta gttggaaaac taacggtgcc
     2221 tttggctgtt gccacagtag gcggagcttc caatatcctg ccaaaagcaa aagcatcttt
     2281 agctatgtta gatattgatt cagccaagga actagcacaa gtaattgctg cagtagggtt
     2341 agctcaaaac ctggctgctt tgcgtgcatt agttacagaa ggaatccaaa aaggccacat
     2401 gggacttcaa gctcgttcat tagcgatctc aattggggct attggtgagg aaatcgagca
     2461 agttgcgaaa aaattacgtg aagcagaaaa gatgaaccag cagacagcta tacaaatatt
     2521 ggaaaaaatc cgggaaaaat aaaaaagcaa tatctttaga agaataaagc aagatttaca
     2581 gttattttat ttactgcaga tcttgctttt ttctctatca gaaaatatgt ttattattag
     2641 aattttttat agttaaggtt aggcatctta agaaatcagg caactccaag atatcttcag
     2701 gtagaagaga agtcgcctaa cattgtagga tcataggtga tttgttggac aggtcaagct
     2761 gatatttgag cctgatgtta gaaggaattt ctttttactt gctgtagaaa ttattatatg
     2821 actaaaaagg ctgggatttt aaatcccagc cttttatgac aaatagaatt tttatcgatc
     2881 agtaatcgct gtcgctgcaa attgttcgat caaaggaatc aaagtcaatt tgactttgta
     2941 tttccgttcg agtgcttttt cgataacgaa ctcattttca gtagtcatag aaatagttgc
     3001 taatttacga ggttcatcag catttttttc attaataact acttcatgtt cattttgttc
     3061 tgtgtaaaat ttaggatact cagcaacggc ttttgaaaaa tcagcaaaac ttaaagcggc
     3121 attcgtatca gttgagcgtg tattttcagt tgtgtccgct tttggttttt ccgttttttc
     3181 ttctggcaca ctgactgttt cttctgtaac tggtgtcgca acagcttgcg cagttggttc
     3241 atcgtttttc aatcgttgat cattccagta agacacgatt tcaataagaa gattgtcacg
     3301 gaatcgttcg aaaatcccgt tttctcctga acgaattctc tttaatacag tattgaagtt
     3361 gtcatcgatt ggcacgttga tccgtgtatc ttcatttgtt ttcccagcta atccgatcgt
     3421 taagatttga cggtttttgg gatcaagcat gacttgtaac ggcaaaccat aacgtcgcaa
     3481 gctctcttct tctagagaac ggaataattg tcctcttaca ccatccaatg aaggacggaa
     3541 ttgatttaat aatttttgtt tctgtttttt cttcatatgt tctctccttc gctcataccc
     3601 tttcgattat aagaaaaaaa cagccaaatg aaaaggagta atgcaaaaaa acttgcgtat
     3661 acgcaaatta caacagaaaa atttgcgtat tttcccgttt tttgaaagaa aaataaaaaa
     3721 tggaacggat tatgtatata tacataatcc gttccattct ttctattttt tcggtatcaa
     3781 ggcaaaaaca ggtagttcag gtgaataaat gacttcatgt tcgtgccagg gtttgaccat
     3841 tagcgaatga tcgaaccgtt ttttcagttc ttcgatgatt tgatcatgtg tccagtttct
     3901 tgcaggaaaa tgttggattt tctctgcatc gatcaaaaag acaaaatcgg aatgaaaagt
     3961 ctgggtaact tcttccgaga gagaattcaa gcgttcgatt ggatccataa aatcaccttt
     4021 cgacttaaaa ttgggtagta tctgggtctt ttgctttgcc gactacacca tctaactgat
     4081 cgatttgttt catttcttct tctgttaatt caaaatcaaa taattcagta ttttctttga
     4141 ttctgcttgg tgtcactgac ttaggaagag gaaggaattc gtgttgcagc gaccaacgca
     4201 aagcaacttg tgcaatcgtc ttgtcatgtg cttcagcgat ttgtttcatt tcaggcacat
     4261 cgaagatttt accagtgcct aatggactat atgcttcgag gatcatatca tgtttttttg
     4321 catactcaac tacagttgtc tgtaattcgc ctggtgcaag gaaaacttga ttgaccatcg
     4381 gcataatttt tgctgttttt gctagtgtat ctagatggtg gggtaagaaa ttgctcacac
     4441 cgattgcttt gattttacct gcttcataca gttcttcaaa tgctcgccat gtatctgcat
     4501 ttgcttgttc ccaattatca cgaaaagcaa cagggtttgg ccagtggatc aaaaacagat
     4561 ctaaataatc tgtttggagc ttcttcaatg attcttcaaa agaagacatc acaagttcat
     4621 agctgtggtt tgcattccat aattttgttg tcaagaatat ctcttctcta ggaattccac
     4681 tttccttaat cgcttggccg acgctttctt cattcttgta tccttgggca gtatcgatat
     4741 ggcgataacc tgcagccagc gcttctttaa cagaggaaac agcgacatcc ccatcaggtg
     4801 tttgccatgt gccgaatcct actaccggga tttcgtaacc atttgataga cgataagtgc
     4861 ttgttaatga attcatactt tcagctcctt ttttattcgt tcaactataa gtgtagcgca
     4921 tttttcgaaa aaaagaatcc attttgtttc tactcttgaa atttttgttt aaaagaaaat
     4981 gattttgatt ctggtatttt tgattttttt tccgtaaact aagacagaag ggaatgagaa
     5041 aatggatatc tatttaaaaa aagcaattct gcatattatt gacagagaaa ctggttctcc
     5101 agtcttttca caaaaagagc tggatctgac aaaagaatat attcgtgatt ttcttcaaaa
     5161 gaagatccaa aaaatctctt ctgctcaaac aaaaactgga caactagcag aagaaagcat
     5221 gatcgttgaa tcacttaaaa tcgctcaaga aaattttgtg gaagcaagtg aagcaatcgt
     5281 tcagcggtgg ttcgatatct accaagaaag tgaagaagca cctagtgcag atgtatttgt
     5341 tgtcttgtat gaattagata cagtgatgta cttatcttta ttgaaagtca attatcgtga
     5401 ggcttacact cactttgttg aagcagacga agcagggatc gacaaccgct tgattttgaa
     5461 tcgagcaatt ctaggggcga aaacacaaaa agcagatgaa gcattcgctg tgaatttgac
     5521 tgattggacc tacgaattaa ttgaaaaaaa atatgagttt tctggcaaaa aagagcttta
     5581 tttttctgga agagtgatcg aaagtgttcc tgcaccttct ttagaagaaa atgtaaaagt
     5641 gatccaaaaa gtagccaagc atcttggaaa aaaatttgag acagaagaat ttgatatcat
     5701 ggctgatttg aaagaagctg tctatgatac gatagaagaa aaaggtcggt tggatcatga
     5761 gatgattgcg gaaaaagtct tcaaggaaaa catcacggca aaaatggctt ttaaagaaga
     5821 agtacaagag caaggatttg tcccagaagc acctcctgta aaagaagtac aagaaatttc
     5881 tgagaaaaaa tttggaaaac aaaaattcaa gctatcaaac gggatcgaac tgatcgtacc
     5941 agtagatgta tatcgcaacc cagatttgat tgaatttgtg aataatccgg atggtacgat
     6001 atccgtgatg atcaaaaatg tggatgaagt attgaatcgt ttataaaaat gactagaata
     6061 ttaggtgttc aagtaccagt gatcagttaa atgaattgtg tcaagagaag gatcggaaaa
     6121 gacgaatata acaaaaaaag agcgtgagac aaaagtaaag aacacttttg tctcacgctc
     6181 taaacacgta taaacggcgg aagcagaagc aactccttct tatttcttgg agttaaacgc
     6241 ttctgtccca gcctcttttt atctaataaa attttaagac catttactaa ttatcgattt
     6301 tttctgccgc atttttaggc acttcagaag cctttatttc ttcccagctt aatacacctt
     6361 tacgaggatt gactttgagt ttaagatacg cattcatctt taatggatgt tctctttctt
     6421 cgcgaagcgt cacctgtttt ttttcaccat ctttattata agccgcttgc ttgtaggtat
     6481 aagtcgtgaa tttctctccg ttatctgctt gggtagctga catttcgcca ttcgtcgtga
     6541 tttttgtata ataagcttcg ccgccgtaaa aataatgata agctgcaaaa cttccgccga
     6601 gaaatatgcc taaaacgaca agtacgccaa ttaatttttt catgtacttc acctctttcc
     6661 ttattattaa cgaaatagaa gatttcgtct attgaaagtt gttggcaaca gctgaatttt
     6721 aaacttttcc tcattttgtt cttattgctt caaaagaaag ttaaggacta tcttgaagtt
     6781 tttctcaaaa aacacatttt tttatttata aaaggtactt ttttcgcgta taagacagac
     6841 gagtgctctt agttgacttt gaccaatatt gacgtataat aagcttagtt cgaatcttac
     6901 aaaaagtaag tcggacgtat tctttttgat ttagtcaaaa aaagactata ataaaaaggt
     6961 gagagctgaa atttttcagt gatctatatt atttaggagg tttgtaaatt atggcaaaag
     7021 aacattatga cagaagtaaa ccacacgtta acattggtac gattggacac gttgaccatg
     7081 ggaaaactac tttaactgct gcgatcacta cagtattagg caaaaaaggc ctagcaaatc
     7141 ctcaagacta tgctagtatt gatgcagctc cagaagaacg tgaacgtggg attacgatca
     7201 ataccgcaca cgtagaatat gaaacagaaa aacgccacta cgcgcatatt gatgcaccag
     7261 gacacgcgga ctatgtgaaa aacatgatta caggggcagc acaaatggat ggtgcaatct
     7321 tagttgtttc tgcaactgac ggtccgatgc ctcaaacacg tgaacacatt ttattgtcac
     7381 gccaagttgg tgtaaaatac ctgattgttt tcttgaacaa agttgattta gtcgatgatg
     7441 aagaattgat cgatttggta gaaatggaag ttcgcgagtt attgagcgaa tatggtttcc
     7501 caggcgatga cactcctgtg atcaaaggtt ccgcattaaa agcattgcaa ggcgatccag
     7561 atgctgaagc tgctattatg gaattgatgg atacagtaga tgaatatatc ccaacaccag
     7621 aacgtgatac agataaacca ttactattgc cagtggaaga cgtcttctca attacaggtc
     7681 gaggaactgt tgcctcaggt cgtattgatc gtggtgctgt tcgtgtcggt gatgaggtag
     7741 agatcgtagg gatcaaacct gaaacacaaa aagcagttgt aacaggtgta gaaatgttcc
     7801 gtaaaacgtt agattacggg gaagctgggg ataacgtagg cgtgttgtta cgggggatcc
     7861 aacgtgacga tatcgaacgt ggacaagtac ttgctaaacc aggttccatt actccacata
     7921 caaaattcaa agcagaagtg tacgtgttga caaaagaaga aggtggacgt catactccat
     7981 tcttcaacaa ctatcgtcca cagttctact tccgcacaac tgatgttaca ggaacaatca
     8041 cattgccaga agatacagaa atggtcatgc ctggtgacaa cgtgacgatt gatgttgact
     8101 tgatccatcc aatcgctgtt gaaaacggaa cgacattctc aattcgtgaa ggcggacgga
     8161 cagtaggttc aggtatcgtt acagagatcg aagcataaga aacgcgactc gaatattttg
     8221 gtaatgaaaa atgtttaaaa taaaagagcg gtccaaacag gatttctgtt tggaccgctc
     8281 tttgttcttc aattgtataa gaagagattg tagaagcatg aactttcgtt ttacttcagt
     8341 ctcttttatt catcatgaga gtgttcatct atcggtatgc agtttttaat actttcttcg
     8401 tatccatttt ttcaaaccac ttattctacc ttgtgtctgc tcaactggcg cattatcaat
     8461 aatcagcaaa gcaatcataa catatgcaat ccaactatct ctagaatata gagtataacg
     8521 tgagacccag tttgtagcgt acttgctttt atattctctt aatccatgga aagaataaaa
     8581 atgagaacca aactcgtaaa ccaagtatgc aatcctttcc tgtaaaaaac tctttctgga
     8641 agttccgaca tttgaaagag gggccatccc taaattgaac cattggatat gttcttcttt
     8701 catataggca aacaaatgca agaacaagaa atccatactg ccagaaggtg cttttttaga
     8761 atcatatcgc atcagatcaa tcgttcctac tttattgttc gtgtaagtag gcatgatcgt
     8821 tgcaaaagcg acaatttctt cttgggtatt tttgacaact gcaataggcg ctctttgtag
     8881 ataatcttca gagaaaaagc ctaaagaaaa ccctttttcc tttcgtgaac ccaaccaatt
     8941 atcagagata tttttgaaag tactcatttg ctcagtagaa aatggtggtt gtaagacatc
     9001 aaatgtaaat ccttctcgtt cgatccgatt caagacagca cgagtgccct tcattttttt
     9061 ccctgaagtt gtaaaactat tcaggtctac ataagcttct tcccccatct taatgaaatc
     9121 gtagccgaat tcatgcaaaa tcataacaat ctcttcgctt gtttcgtaga aaactggtaa
     9181 atagcatagt cgatctgttt cttcgataaa agcttcgatt gcttctggaa aatcttcttt
     9241 cttaccagaa ggatcaccca ttacaataca tttattattg aagcaagcga attggagaag
     9301 aacagttggt tcaccgtctt tttcataggc aaacatccgc ttatctttta agaagatcaa
     9361 ttgactatcg gagtttcctc cataagttgt aaggatcttc agggcctttt cttcattaaa
     9421 ggcttcgcca atctgtttct tttctccttg aaggaaatga acaaatagaa cgatcatgaa
     9481 actaacagcg atgatggcca acaatcccga aaaccaaatc ttttcagaag gaaataagaa
     9541 aaaggaaata aaatggtgac gccgatgagg gaaatccggc agattgtata ccccaataac
     9601 gatgtaaagc aaggacagtg caccaataat gattccatcg atcgtacgcc actcccatga
     9661 atagatcaat tgttcccgaa ataattcgtt tttagaagca agaatgatca gaagcagaat
     9721 agacaagaaa accaccggtg tgaaactaaa atcacttaat aaaacataaa gtaaagctag
     9781 aacaatcaag aagattgtag gcaaatatgc tcgtttcaca cgtgctgcga tccctctgcc
     9841 cataatgatt aaaagaaatc ccaatagtat actaggaaat tggataataa aatgaaattt
     9901 caatgggttc aaactatgaa gccagcgaaa ctctgtaaaa gcttgcggga tagtggcaga
     9961 aagtactagc atgattccag aaaagtataa taagacaacc acgattttat gcgcaatttc
    10021 tgttgctaat tgtttaggga taccagaata acgctgatca aaagtggaac cgatattttt
    10081 gaagaaaaag acgattccaa tcaaaaacgg aatgatgtag tagaacaggc gatagagcaa
    10141 gatccacaca acgactattt ctcttggcac accaatagcg gacaacccga taatcatcat
    10201 tacatcgaaa ctcccaattt ctcctggaat catagacacg attcctatca ctgaagcagc
    10261 aatgaataat ggaattgttt gccacagatc aatccggatt tccattaaat aaccaacaga
    10321 aaggaaacta aacaggacac cggtccattc caacaatgaa actagcaata gctgtccttg
    10381 agttttaagt gaggtaccac caaggaatcc tttctttttg aaagtagtaa ataaaaagac
    10441 gacaggaaaa tatagaccgc cgccgatcaa ccatatccaa tattgcttca aaaaaggagc
    10501 aactggcgtg aagactacaa gtaaaaaaga aagcagactg taaatagaaa gtccagccat
    10561 caaaaacaag aaaactttcg ataatgcttg gatcacgttt ctgctctctt tcttttgtcc
    10621 atatagttca gagcgaagcc ccacactaac aaacccgccg aaaccggcaa tattgttgat
    10681 cgtattgatc agccagcttg tctcaaacag gtaacgagga ttctgctttt gttcaagcat
    10741 ccgattcaaa ataatatcat aaccaaccat aggtaacaca gagataagac caatcaatag
    10801 cattagtccg cttttccata caggtatagt ggcaaacgtt tctcctaatt gctggataga
    10861 tagagtttta ccgatggaca tcaattcacc gacaataatg acaatgacag aaatcaaaaa
    10921 gattgttttg aacagtccta ggtggttttt cagccattgt atggtatttt ttaacaaata
    10981 aatcaccttc tattcctaat agttatgttt cagaaagaaa ttgaagaact tctcgattga
    11041 agcgttcagg atcttttcta gctaattcat gtccttgttc tttcacaagc acaaaggaag
    11101 cttttggtat agttttagct atatacaaag aatgttttag cttgattaca tctttttttc
    11161 ccacgatgat caaagtgggg gagttgattt tttttaaatc attttctgta agcccgatgt
    11221 catgcagcaa aagtttgata actaaaagat ttttgcgaag acttgggcga aataaagaga
    11281 gtaaccatac ccatgcataa tgaatattac tgatgactct ggcagaaaaa cgaacgcctt
    11341 taacaagcgt gtttccagaa tttaaaatca atcgatgaac ttttttaggg aagctgctgg
    11401 caaataccag tgctagattt gctccatcac taaatccaag aaaatctgcc tgatcgattt
    11461 tttccagcaa catgatcgta ttcagatcct cggccattaa ctgaaaattc aacatagatg
    11521 cttcgtttgt cgaacgacca tgtcctcggc tatctactag atacacggta tagtagcgtt
    11581 cgagtacagg gacctgttct gaaaaaaagc ggccgctgcc gtcattgcca tgcagtaaaa
    11641 agagagggaa gccttgaccg ctcttttcat agtaaatttc gctcccatct ggcatttttg
    11701 catatttctt ttcttttttc acataatcac ctactgtgta aagtataacg attatatcag
    11761 tcattgggca attgttttta ctgaaaaaca acgatcattt tcgtaagatg tcaaaaaata
    11821 caggaaaatt cattttttct taaggcgtac acagaaatca aggaagaaat agccaagaag
    11881 ataaagaaat agtaagataa aaaaagatgt caaaataact aggtgatgaa atgaagcgaa
    11941 aacgattatc tattatccgt attctttttt acatgatcat tctgacggtt attggtgcgg
    12001 tgttgttttt atgaggaaga acttatcgtt cggtaaaacg agtggaaagc tatcaaatag
    12061 aaataacaac agctgcaaaa aattatcata tggaaaacta cgagaatttg atcaaggcga
    12121 taatattgac cgaatcaaaa ggaaaaggga cagatctcat gcagagctcg gaaagtctgc
    12181 atggagaagc tgagatcatt gataatccag ctgacagtat tgatcaaggt gtcaaatatt
    12241 tggcggaaat gatagtagaa gcaaagaaac aaggatgcga tttatggaca gctgtccaag
    12301 catataattt tgggaaagat tatattgcct atgtaaaaaa tcatggcgga gaaaacacag
    12361 tagaattagc agaagcgtat tctaaagatg tgctgagccc acttttagga aatgaggaag
    12421 ggaaacaata ccgatattgg cggatccagt ccttgcttta caatggcggc tatctctatc
    12481 ataatggagg taacatgttt tatcctgata ttgtcaaaat gaatgaaaaa ataatagaga
    12541 cctatgaaaa agtctttaag taaaatagca ggaaatgttc aatcatgaat aaaattaaat
    12601 gaaagaagga gtgcgcaaat agtggaaaaa aggcaaaaaa tggaaaatca agaagtgggg
    12661 tacacaacca aagactggat gttacggttt gtgaagggaa tgtttatcgg ttcaggattt
    12721 atcttgccag gagtcagcgg tggtgcatta gcagctgtct ttggcatgta tgagcggctg
    12781 atttcttttt tagcccacct tacaaaaaat ttcaaagaaa atgttttgtt ttttctgcct
    12841 gttggtctag gcggagtgac aggcatcttt ctactttctt ttttagtcag ttttttatta
    12901 ggtgcgtatg aaacaacgat attatggttt tttgtcggat gtatcgctgg aactgttcct
    12961 gcgttatgga aagaagcagg gaaaaaagga agagatactt ctgatgtgat catcatggtt
    13021 gttacttttg ttctggctta tttgttttta ttatacggcg ctcaattatt tgacgggcaa
    13081 gtcgatcaga attttggaac atggctgatt gccggcggat tgattggttt gggtatgatc
    13141 gtcccaggat tgagcccttc taattttttg gtgtatatgg gattgtataa agcgatggct
    13201 gacggattta aagaagtacg acttgaagtg attgtgccga tcggaattgg tgggattctt
    13261 tgtgttcttg gattgtcaaa aatcatggat tacattttct caaaggcata tagcaagcta
    13321 ttccatttca ttttaggtgt agtttttgct tcaacgatta tgatcattcc taccgattac
    13381 gcgggattgg gagttgctgg ggtactagct tgtcctgttc tttttatagc cggtacagca
    13441 ctaggttggt ggatgagccg tttggaggaa caatataagt aaagatagat tgataataaa
    13501 aacagcacac gggtgattca aacaaaaagg ttggcatctt ttttgtaaaa tcattcgtgt
    13561 gtttttctat aaattttata aaaacaagtt gtttttaatc ttggaacacg ctagacttaa
    13621 acacgtgatt atttttgaaa gttggcggaa aatggagaaa aagaaaatga atggacttac
    13681 acagcaagag gtagttcaga aaatggcttc tggccagcaa aatgactacc aggaagatgc
    13741 atcaaaaagt acaaaacaaa tattcagcga taatatattg acgttattca attttctgaa
    13801 ctttggtatt gggatttgtc tgttatttgt aggtgcgtat tccaacatgg cgtatttagc
    13861 aattattgtc gtcaatatcg ttattggaat ttatcaagaa atacatgcaa gaaatcttgt
    13921 acggaaatta tcgattgttt cccaatcaga agtactagtg atccgggacg gtaaagaaca
    13981 atcgatttct actaaagagc tggtattagg agatttggta aaaatcggag caggagaaca
    14041 gataccagcc gatttacagg ttatatctgg acgggcagaa gcaaatgaag ctcttttgac
    14101 aggcgaatct gatttgattg tcaaagaaac tggagctgaa ttgctttctg gaagcttttt
    14161 gacaagcggt tctgtcatgg ctgaagttat ccgagtaggt gcagataatt atgctgtccg
    14221 cttgactagc gaggcaaaga ctcacaaagc tattcgttct gaacttgtag attcgattcg
    14281 caaagtatcc aaattcacta gtttcgtgat tattcctttg gggatcatac tatttttgga
    14341 agcactgttc atccgcgatg cttctatcaa aatttctgtt attgcatctg cagcagcttt
    14401 gttaggcatg cttccaaagg gtttggtatt gctaataagt attgcattaa cgacaggggt
    14461 aaccaaactg gcaaaaaaac gaatccttgt tcaagacatg tattcaattg aaacgctggc
    14521 ccatgtcgat acattatgct tggataaaac agggaccatt actgaaggga aaatgagtat
    14581 ccagcgggta gaagcactac atccgctgta cgagaaagcc atcccagaaa ttatggggac
    14641 gtatctggca gaaagcagtg ataacaacat cactatgcgg gctatgagag attattttga
    14701 gctttctaat cggtatgatg tgacaaacaa agtcgcattt tcttctgatc gcaaatgggg
    14761 cgcgatggaa tttccagaac tgggagtcgt gtacgtagga gcgcctgaac gtttggtgga
    14821 agaagatcgg cttccggaca gtctaaaaac agcccaagcg aatggctatc gcgtacttat
    14881 gttggggatc gctccaaatc agcaattgaa cgaagaaaaa ccagcatttg ttgagccggt
    14941 tgctctgttt gaaatcgatg atccaatccg aaaaaacgct caagaaacac tagcttatct
    15001 tcatgaggaa ggtgtggatc tgaaagtgat ttctggagac aatcctttta ctgtctccaa
    15061 tatcgctaga cgtgcaggat taccgaatta tgatgcgtat gtcgatcttt ctcaaattac
    15121 tgaagaaatg gaagtgcgag aagctgctca tcgctatacg gtttttggac gagtgagtcc
    15181 gcagcaaaag aaattgctgg taaatgaatt gaaagaaagc ggcagaacgg ttgcaatgac
    15241 aggcgacggt gtgaatgatg ttttggcgtt aagagaagca gattgcagca ttgctatggc
    15301 agaaggtgat ggagcaacac gtcagattgc gaatctggta ttgttggact ctgattttac
    15361 tacattgcca gaagtcctgt ttgaaggacg ccgagtggtc aacaatgtga cgaaagtctc
    15421 aggcatcttt tttatcaaaa cgatttattc atttatttta tcgattatat gtgccgttac
    15481 agcaattgga tttccgttta tccctatcca aatcacgctg attgatttag cgattgaagg
    15541 gtatccttcc ttctttttat cttttgaaca agataaaaga aaaatcactt accgttattt
    15601 gcctacagca cttattaatg ctttaccaaa tgccttgctt gtagtagtga atatcattgc
    15661 ggtgtatcta cttggaaaaa cattcgcatg gggaaatgct gaaactacta ctttgatgta
    15721 ttacttgctg attgggatca gttgcatggc agttgtaaaa gcttgttatc ccttcaaccc
    15781 attgcgcatc ttcctagcag taacgacgat cgtagggata tatgtagctg caatgctgtt
    15841 ccatggaatt ttggaagtaa acttagggtt gggagagagc gtaggatact tctttgtatt
    15901 catgctcgta aatattgttc ttcgagtatt gtactggaag gctgatattc aaaatcagtt
    15961 actgaaaaga gtagcaaatt gatgcttaat gagatagaaa tgtgttatac ttgaaaaagc
    16021 aatgaaatat gatacaatca aacatgtata attgtgtcct ttaaaatcgc actgatttca
    16081 gttagtgcga ttttttaccg gttattctcc tgaaatatct tctgttaaac ccaaaagata
    16141 tcaatagagc agggggtgaa aaaatggctc aacggattcc tcaagaggtt atcgaaacag
    16201 tacgcagtca aacgaatatc gttgaggtga ttggacaata tgtccagttg aaaaagtctg
    16261 gtaaaaatta cttggggctg tgtccttttc atgaagaacg tacaccgtct ttttctgttg
    16321 cagaagacaa gcagattttt cattgctttg gttgtggtaa aggcggaaac gtgtttactt
    16381 ttcttcaaga actagaaggt ctaagttttc cagaagcagt ggtaaaagtg gcagacttcg
    16441 aacagatacc gttagaagat caatggcgtc agcgtactat agctgtccaa aatcctgaat
    16501 ctgctacggg gaaactgatt gctgctcatg aaaaagcagc ggaaatctat catcacatgc
    16561 tattgcatac aaaagctgga cagccagctt tagaatattt gcaaagtcgt gggttgtctt
    16621 tagaattaat tgaagaattc aacataggtt ttgcacctaa tgagcgttct tttcttcagc
    16681 aagtattcaa gaatgaatca atttcaaatg aattgttaga acgctctgga ttgtttgtca
    16741 cgcatgatga tggccgtatg tctgatcgtt tttatcaaag agtgatgttt ccacttcgta
    16801 attctcaagg ccagacgatc ggtttttcag gacgctggct tgcacctgaa aaagggcagg
    16861 aaaaagatca gcccaaatac ttgaatagtc cagaaacaga actgttcaat aaaagacaag
    16921 ttttatttaa tcttgataaa gcaagaagcg atatccggaa aaacggagaa attttgttgt
    16981 tcgaagggtt tatggatgtg attgctgcat ggcaggctgg tatcaaaact ggattagctt
    17041 ctatggggac aagtctaaca gctcaacaga tccaacaaat ggaaaagctg acgaaagaat
    17101 tggtcttttg ctatgacgga gacaacgcag gttttgaagc aaccaatcga gggattgaat
    17161 tgcttcggca aaacagtcga ttagcacttt ctgtcgtagt cgttccagaa aagcttgatc
    17221 ctgatgaata tctccgtaag tatggagaag catctttcca agaattagtg gctcatgggc
    17281 gcgaaacagt ctttactttt aaatcacgtt atttaaaaca agggaaaaat ttagataatg
    17341 aaaaagacaa gatcgaatac ctcgaagaat tactagttga acttagccga gttgtttcac
    17401 caatcgaaca agatatgtat ctatctcaac tgtcaacaga gtttcagatc ggcagagaga
    17461 cgatacaaaa acaactgcgc gatcttcgaa ggacaaatca gcaaaatcgt caagaacctc
    17521 aggaaagtca acacactcag gttacgagac aagaaactgc acggaaaaag agacctttga
    17581 cgcaagtaga aaaggcagag cagattctat tatatcgtgc atttaaagaa gtttctgtcc
    17641 gtaatatttt aaaagaacga gaatttcagt ttattcatga tgcatatgca gaagtttatt
    17701 ttttattcga tgcatatgtc tcacaacatt atgaattcaa ccttgcagag tttttagact
    17761 ttttgcaaga tgaaaattta aggcggttgg tggtggaaat cgaatattta ccggtggcag
    17821 aagaaagttc gccacgagaa atccaagact tgctagatgt gatcagcaaa tctggcttag
    17881 ctgatgaaat aacattaaaa aaacaaatgc agcaagaagc cagacgaact ggaaacaaac
    17941 agcttgagct tgaattgacg attgaaatca tcaatttaac gaaacagtta aaacaagcca
    18001 aatagctact caaggggggc cttcttttat ggcaaatgga acagataaaa aaacgtatga
    18061 agcagcagta gcagctttca tcaaagaaaa caaacccaaa cgacaagttg tatatgatga
    18121 tttatccaat cagcttgcta caccattcac tttgaatgca gatgaaatgg acaaattgat
    18181 ccaaaagatt gaagacgcag gtatcagtgt ggtggatgaa aatggtgatc catcaattca
    18241 cagcttgaag agtgctgaga aaaaagctga acaagcaaaa acagaagatc tttctgcgcc
    18301 aaccggtgta aaaatcaatg acccggttcg tatgtatctg aaagaaattg gccgtgtctc
    18361 ccttttaact gcggaagaag aagtagcgtt agcattgaaa atcgaagaag gcgaccaaga
    18421 agcgaaacag cgtctggcag aagcaaacct tcgtttagta gtaagtatcg ctaaacgtta
    18481 tgtaggacga ggcatgcagt tccttgactt gatccaagaa ggaaacatgg gactgatgaa
    18541 ggcagttgaa aaatttgact accgcaaagg atttaagttt tcaacctatg ctacatggtg
    18601 gattcgtcaa gccatcactc gagcaatcgc tgaccaagca cgtacgatcc gtattccagt
    18661 tcatatggtt gaaacaatta ataaattgat acgtatccaa cgtcaattgc tgcaagacct
    18721 tggaagagaa cctacgccag aagaaatcgg tgcggaaatg gatcttccaa cagaaaaagt
    18781 ccgagaaatc ttgaaaatcg cgcaagaacc tgtttccctt gaaacgccaa tcggtgaaga
    18841 agatgactct cacttaggtg atttcataga agaccaggaa gcaacaagtc cagcagaaca
    18901 cgcagcttat gagttactaa aagaacaatt ggaagatgta ctagatacat tgactgatcg
    18961 ggaagagaat gtccttcgct tacgttttgg tttagatgat ggccggacga gaacattgga
    19021 agaagtaggg aaagtatttg gtgttactcg ggaacgtatt cgtcagatcg aggctaaagc
    19081 attaagaaaa ttgcgccatc cttctcgttc aaaacaatta aaagatttct tagaataagc
    19141 gtggactatt gaccagaaga gaaatcagta aaactgtttg gtttatttta aaagaaaaaa
    19201 agcttgagac tacgcagtct caagcttttt tttctatagg aaggaaattc attcattatt
    19261 tttaagtaaa tcctaaaaat aagctattgg cgtgacgtat agaatatgat agattaaatg
    19321 gtagcgtaaa cctagtaaag gagccgatcg ctaagtgatg aaaaaatgtc caaattgcgg
    19381 atcgaaaaaa atggagcaga atactatctg tccagactgc ggtcatcaaa taactgcaga
    19441 agatcagcaa ataaaagaat caattgaaga aacaaataca gatgcagcta tgcaggataa
    19501 atcaactgaa gatgcagtct ttcctgatac agaattaaac gatccaatcg aatggtcgga
    19561 attgaaagat ttacctctag aatctgtaat ggaattattc gaaaaagcag aaccagaaga
    19621 cgaagaacca cctgctcacg atcaagtaac taatatagag aaaaatgaac agaagaaagc
    19681 aactgttcat gagacttcgg aagaatcagg aaagaatcaa gaagaaacgc ttgaatcaaa
    19741 acaaaaaaga agagtagctg aactgaaaga aatcgtcgat aatgaggaag aaaattcgat
    19801 actatccgct tatatcaaag cacatcgtga agatacaaaa gaagagcacg cgaaagagct
    19861 gctcaaaatg atcagtgaaa aaattgcaca ggaaaacaaa gtagagccag aaaaggaaca
    19921 agaaactgaa aaagactctg atagagaaaa cgccacaggt aaagaaacag taaaaataaa
    19981 agacgtaaaa acggatatca cagaaaaaac tgaagtattt actgaaaaac aattagaaaa
    20041 ggaaaaaacg aataaactgc cggaagcaga gaaaaaagaa atactcgaaa acacagacaa
    20101 aaagtcaaca gagcccgatg aaataaatag tgaatccgat gctgaaaatc aatctgtaag
    20161 caaagagggt actgatcttg aagcaaaaat agaaaaagaa gagaaccagc ctaagctgtt
    20221 tgaagcagaa gcgaaatcaa taacaatcgt gccagatact gaaaaaacac atcaagaatc
    20281 aaacttagaa aaaacagatc cagataaagc gcaaaagaaa aaatcaaaaa aagtacctta
    20341 tctcgtttta gcagcagtac ttctactcgg tggaggaagc tgggcttatt atgaccatca
    20401 tcaaaaagta gaagccgaag tagcagcaga agcaaaaaga cagcaacaaa tggaagaact
    20461 tcatgagaaa cttgctgcct tttatacaga tgatcaccat caatttattc gtgcagatat
    20521 gattcatcaa gatttatcaa aactaaaaaa tgatttatcc agtcttaaga atgaaaaagg
    20581 atattcagag ttagaaacaa cttaccaaga tatccaatct aagatccgag atatccaaaa
    20641 agtgaatgag tggttcatag cacctgccat cgtcgatgat catctagcag atgacatcaa
    20701 gttgaaagca gataaagaga tccaagcaat cgaaacggat aacacagatt ttggaaaatt
    20761 gattcaacaa gcacaaacag aagctaaaaa tcaatatgct gaattgcaaa cagctaaaga
    20821 aaaaacagct atagtcttta aagacggaaa agtagtggat tcagcaacac gcgaccaata
    20881 caagacggca aaagaagcag tagaaaaagt gaaaaataca gatctatctg ctcagttgaa
    20941 tgaacaattg aaacaagtag ataagatgct cactgataaa gagaaaaaag aagaagagga
    21001 aaagaaaaaa gcagaagaag cgaaaaaagc agctgaagaa caagcaaaac aagccgctca
    21061 agcagcagct gcagctcaag ccgctcaaca agctgcggtc gcacaagcag cacagcaaga
    21121 agcggcatcc tcgacccctt cctcttcaaa ctcagcgaat cagccaatca aatctgccag
    21181 agcaagtgat attgccgatg catctaatcc agcatggacg tgggcaccag gtgtccaaag
    21241 caaagtgatt gctacatgta tccaacgtgg atatatcgtt gaaggaggtt acagattaga
    21301 aaaagccaga atcgaaaatg gggaaggcta ttataatcta tatgccactt caacaaaatc
    21361 tgcattgatg aaaggcattg gagaaagtgc tttgccattt tacatcgtaa ccatcaactg
    21421 taaaactgga tggttcggag gaaatgggag caattaattt taggcaaatg aaagaggctg
    21481 tgagcctgcc gtttaacacc gtttattcga aaaaggggag tatgacacaa ctgttgtcat
    21541 actccttttt tcacctttcc aaaccaaggc tcagctattc aagtagataa cattatgtta
    21601 tgatagcaaa gaaatgtatt aagaaaacct ttcactatag agggagaaaa aaatgaacga
    21661 tttattagca actatattta caggtattat tattgatgaa aatgaaaaaa actactttgt
    21721 ccaaaaaaac ggacaaacct ttggcttaag caaatcagaa ggtgaacaca aaatcggtga
    21781 agcagttgaa ggattcggtt accaaaatca aaaacatgaa aatcggatca ctacacaaat
    21841 tcctaaaagc cgtatcggtc attacgcttt tggaatcgtt actgctacac gaaaagattt
    21901 aggtgcgttt gtcgatatcg gtcttccaga taaagacttt gtcgtatctc ttgatgaatt
    21961 gccgacaatg acagagctat ggcctaaaaa aggggatcgc ttgatgattt cccttagagt
    22021 tgatgcaaaa gaccgtattt ggggcagttt agcagatgaa aaaatattca aatctttaag
    22081 caaatatgga acagaagaaa tgaaaaacaa agatgtgata ggaacagcat accgcttgaa
    22141 attaacaggt acgtacgttt taacggaaga tttctatctt gggtttatcc atccttctga
    22201 acgatataat gaacctcgtc taggcgagca gataaaaggt cgagtgattg gtgtccgacc
    22261 agacggtgta ctgaatcttt cattgaagcc aagagcatat gaagctatat cagatgatgc
    22321 acaaatgatt ttaaccattt tagaacgttc tgcagatcag caaatcaact ttacagataa
    22381 atcggatcca gaagaaatca tgaaagcttt tggtatcagc aaaggagcat ttaagcgcgc
    22441 attaggtaat cttttgaaac aaggattgat tacacaagaa aacggtgtga caaaactagt
    22501 caaaaaatct aattgagaat cccttgcatg tttttcttaa ctagcttata ataattttaa
    22561 ttaacatagt gttatatctt tttttatttg taattattat aaattgttgg ggttgagtaa
    22621 atggtcatga atgctatttc ggcgatgaaa aaaacaaaac agcagctaca tgaagcgggg
    22681 tttaagctga caccgcaaag agaagctact ctgcttgtgc ttttggaaaa tgagaaagag
    22741 catttatctg cagaagaaat cttcttttta gtgaaaaaaa agagtccgga aattggactt
    22801 gctactgttt atcgaaccct tgaaatcttg acagaattga aaattgtcga taaagtaagt
    22861 tttaatgatg gattagcccg ttatgatttg agaaaagaag gcgccaaaca tttccatcat
    22921 catttactat gcttgaactg cggcacgatc gaggaagtag aagaggatct tttacttgaa
    22981 gtggagcaag tagtagaaga acgttatcat tttttagtaa aagatcatcg gctaacgttc
    23041 catggaatct gtcaggaatg ctatgaaaaa gaaaa
//
LOCUS       DACDXO010000042        23058 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E23605_2017
            SAMN12639989-rid9881463.denovo.042, whole genome shotgun sequence.
ACCESSION   DACDXO010000042 DACDXO010000000
VERSION     DACDXO010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639989
            Sequence Read Archive: SRR10023389
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741914.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:42:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,972
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 33 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 17 of 73
            Pseudo Genes (multiple problems)  :: 10 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23058
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639989-rid9881463.denovo.042"
                     /isolate="E23605_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(109..564)
                     /locus_tag="IWM35_001803"
     CDS             complement(109..564)
                     /locus_tag="IWM35_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9919652.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            complement(718..1926)
                     /locus_tag="IWM35_001804"
     CDS             complement(718..1926)
                     /locus_tag="IWM35_001804"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HAP9919653.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNEGAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            complement(2038..2370)
                     /locus_tag="IWM35_001805"
     CDS             complement(2038..2370)
                     /locus_tag="IWM35_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HAP9919654.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            2518..3393
                     /locus_tag="IWM35_001806"
     CDS             2518..3393
                     /locus_tag="IWM35_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP9919655.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            complement(3475..4056)
                     /locus_tag="IWM35_001807"
     CDS             complement(3475..4056)
                     /locus_tag="IWM35_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IDEAL domain-containing protein"
                     /protein_id="HAP9919656.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(4062..5321)
                     /locus_tag="IWM35_001808"
     CDS             complement(4062..5321)
                     /locus_tag="IWM35_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HAP9919657.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            complement(5323..6369)
                     /locus_tag="IWM35_001809"
     CDS             complement(5323..6369)
                     /locus_tag="IWM35_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9919658.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
     gene            complement(6604..6879)
                     /locus_tag="IWM35_001810"
     CDS             complement(6604..6879)
                     /locus_tag="IWM35_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008214712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HAP9919659.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(7122..8432)
                     /locus_tag="IWM35_001811"
     CDS             complement(7122..8432)
                     /locus_tag="IWM35_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HAP9919660.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            complement(8614..9843)
                     /gene="rpsA"
                     /locus_tag="IWM35_001812"
     CDS             complement(8614..9843)
                     /gene="rpsA"
                     /locus_tag="IWM35_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HAP9919661.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            complement(9978..10658)
                     /locus_tag="IWM35_001813"
     CDS             complement(9978..10658)
                     /locus_tag="IWM35_001813"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HAP9919662.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            complement(10707..11342)
                     /locus_tag="IWM35_001814"
     CDS             complement(10707..11342)
                     /locus_tag="IWM35_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP9919663.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            complement(11408..12811)
                     /locus_tag="IWM35_001815"
     CDS             complement(11408..12811)
                     /locus_tag="IWM35_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HAP9919664.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATKQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWETILLNLFKKNMEH"
     gene            complement(12798..13829)
                     /locus_tag="IWM35_001816"
     CDS             complement(12798..13829)
                     /locus_tag="IWM35_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9919665.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESEVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            13860..14081
                     /locus_tag="IWM35_001817"
     CDS             13860..14081
                     /locus_tag="IWM35_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HAP9919666.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            complement(14246..14983)
                     /locus_tag="IWM35_001818"
     CDS             complement(14246..14983)
                     /locus_tag="IWM35_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HAP9919667.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(15136..16476)
                     /locus_tag="IWM35_001819"
     CDS             complement(15136..16476)
                     /locus_tag="IWM35_001819"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HAP9919668.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            complement(16713..18236)
                     /locus_tag="IWM35_001820"
     CDS             complement(16713..18236)
                     /locus_tag="IWM35_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HAP9919669.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
     gene            complement(18614..19225)
                     /locus_tag="IWM35_001821"
     CDS             complement(18614..19225)
                     /locus_tag="IWM35_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP9919670.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     regulatory      complement(19348..19473)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(19555..20271)
                     /locus_tag="IWM35_001822"
     CDS             complement(19555..20271)
                     /locus_tag="IWM35_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HAP9919671.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     gene            complement(20277..20846)
                     /gene="scpB"
                     /locus_tag="IWM35_001823"
     CDS             complement(20277..20846)
                     /gene="scpB"
                     /locus_tag="IWM35_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HAP9919672.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            complement(20830..21627)
                     /locus_tag="IWM35_001824"
     CDS             complement(20830..21627)
                     /locus_tag="IWM35_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HAP9919673.1"
                     /translation="MASKEKEERVNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDI
                     PIATITEQYMNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFGTDDEELWEE
                     DPREELVTQLLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKK
                     IDLIRAMQKILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESP
                     SKSEVVTTFMALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            complement(21597..21968)
                     /locus_tag="IWM35_001825"
     CDS             complement(21597..21968)
                     /locus_tag="IWM35_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAP9919674.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            complement(21987..22874)
                     /gene="xerD"
                     /locus_tag="IWM35_001826"
     CDS             complement(21987..22874)
                     /gene="xerD"
                     /locus_tag="IWM35_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HAP9919675.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
BASE COUNT         6463 a         4926 c         3910 g         7759 t
ORIGIN      
        1 aaatcttgtc acagtccctt attctgaata aacggtgtgg aagagacagc ctatcagtag
       61 taataagaca catctctttc acagcctcat ttttgcgtaa aaatcgattt attcgaaaaa
      121 ttccccgtat acatcttcta cacgttgatc tggattgatc acgatataca agttacctgt
      181 tttttcgctg atttttgttg attgatattg attgtctaat ccttctgttc ctttatctag
      241 atatgggaag tacacaagat caggtgtgcc atttcggtaa aggatttcca ttcgctcgat
      301 atcttcgtat ttcaatgcgc gattaaacat gcccatttcc agtccgccta ggttgatatc
      361 atgtgtcgca actgcgtccc cttcatggaa gatttcgatt ttaaatccag cacacggatg
      421 gatttcaaca aattcactgc cattgattcg tccaaaactt gttgtgattt gtttgatcca
      481 aagatcgccg atatgacggc ggttgatcgt ccaggtttct ccatttctaa aataaaattt
      541 tagcgctgtc atttctttac tcattaaaaa gactccttta tgatgtaatt tattcttgta
      601 ctacctcagt ttagcacaaa tttatgtaag tggtcacatc ttatgttttt tcttctaatg
      661 acgtatctgc ccactttaag tagccaacaa agctgagcag atacagtcta gtgtttttta
      721 aatcaatttt tctttcgata aaaaatccaa aacctgtttt ttatcatttt ttattctttg
      781 ttctacgacg gctttttcag ctaattgcaa agcttcacta acccacttac caggtttttt
      841 tccagtttct tctagcaaga ttttaccatc tactgctaat tcgcttaatg acttgatagg
      901 taaacgatca taggattctc ctacggcatc catctgaggt gtttcgtcaa aatagatcat
      961 caattcttct acttgcagtg catgtttttt gcctaagaag tacaattcga atggttccaa
     1021 agctccagtt tctcgttgtc gtagtccttg gacaagtgct tgaacgtctt taatcatttg
     1081 gttagaacac ttccacgatt tcaaaaagga acgaatctca ttttctttta gatccagttg
     1141 gctgatcaat aatgcccatg cttggctctc ttctttcaat gggacggatg gtaattcagc
     1201 aaaacgtaaa agtgcttcgc cttcttcttt caaaccggga cagcaaatgt agcactctgt
     1261 ttcaataaat gcatggattc ccgcggcccc ttcatttccc aagagcatct tgatgaattc
     1321 cacatttata cgttctacag atattttacc taatagctcg tggttttctt tgatggcgta
     1381 gaaggtttct tcctctaatt tgaaccctag ttgactgaca aaacgcaacc cgcgcatcat
     1441 tcgtaaggca tcttcatgga accgctcatg aggattgcct acagctcgca agatctgttc
     1501 ttctagatct tgcagaccat cgaacaaatc gatgatttct ccatcttctt tcaaagcaaa
     1561 cgcattgatc gtaaaatctc ttcttttcaa atcttctgcc aacgaacgaa cgaattcgac
     1621 atgatctggt ctacggtagt cctgataagt tgattccgtc cgaaatgtgg tgatctcata
     1681 ttgttcatca ttttctaata ccaacacagt tccatgttcg atcccaatat ctatcgtttt
     1741 tggaaaaagc tgtttgattt cttctggaaa tgcgcttgtt gcgatatcca catcatggat
     1801 tggctggttc agcaagatat cacgaacact tccacctaca aaataagctt catatcctgc
     1861 attctctaat tttttcagaa ctggaattgc cttttggtat tccagaggta attgttttaa
     1921 tttcattttt ccaacctttc ctgcgtgttt cttgtcctga gataaagact cagttgcatg
     1981 gtgactgttt gacaagtccg tttcagagtc ttcaaggcca ggaccacttc atcttgttta
     2041 ctcgtcctta cgctcaaagc gattatggtc tctttgattg aatttttcca tgtttttttc
     2101 aaaaacttca gttaagtcga tattcaatga attagccatg atcatcgtaa cgaataaaac
     2161 atcaccaagt tcttctgcta ccgagttaac tggttcagaa gcctttttgc ttttttctcc
     2221 ataagtgtgg ttgacttctc ttgccaattc acctacttct tcagttaatc gagccatctg
     2281 agctaatgga gagaaatacc ccaccttgaa ttgctggata tattcatcca cttcctgctg
     2341 catactgtat aaagaacgct ttttatccat ctttttccct ccaaaactat cttaccaaaa
     2401 ctttaacgca aatcctagat aagagcaatt gagaaaatcg aatttccatg ctacatttag
     2461 gaaggaattt ctaaatggaa attctgtgaa aatacataat tgaaagctgg aattcatatg
     2521 gtcaaacgaa aaacagtaaa agaagttctt tttatcatta taggaacaag tatttatgca
     2581 tttggtcttg tttacttgaa catcgctaat catttagcag aagggggagt ctcagggatc
     2641 accttgatct taagagcttt atttggtatt gatccagctt atacgacttt attaatcaac
     2701 atcccattga ttttactagg aggaaagatc ctcggtaaac gttccttggc ctatactgtt
     2761 ctaggtacac tttctttgtc attcttttta tggatctggc aaagggttcc actagaaatc
     2821 aatttacagc atgatctact gatcgtttct ctacttgcgg gtttagttgc aggtgtagga
     2881 agcgggatcg tctaccgcat gggtggaaca acaggcggca gtgatattat cgctcggatt
     2941 ttagaacaaa attacggcat cactatgggg cgttccctat tggcatttga tatcatcgtc
     3001 ttacttgctt cattaaccta tatcgacttg aaacgtatga tgtatacgtt gattgtttcg
     3061 tatgttttca gtcgtgtgat tgattcgatc ttagatggcg gatattctgc aaaaggtcta
     3121 cttgtcgtct ccaacaaaag cgaagagatt gcaccactac tgatgaccgg tctgcaaaga
     3181 ggtgtgactt tcctttctgg agaaggcgca ttctctggta attcaaaaaa aatcatctat
     3241 atggtcgtta gctctagtga attaactgaa gctaagagga tcgtccacga gattgacgaa
     3301 aaagctttcc tttcaatcat caatgtgcat gaagtggaag ggcaaggctt tacttatttg
     3361 aagccacaga caagattact gaaaaaacta taagaaaaaa gacaaagata ggctatgagg
     3421 aaagattcca catccagctg acctttgtct tttttagtac tatactattt ttaactaatt
     3481 gttatgttca cttacccggt tacttttctt ttgtgtctgc aaaaggatct ttcggttaac
     3541 ttcatcagat aatttcaaaa aagcatccct atctccatct tctagtgctt gatcgatttg
     3601 agcataaagc atttttagtt ttgcctcttc ttctttttct tgaaaatatc gttcaattaa
     3661 ttcagtatct tcttcactga ctgaagaatc ccaagaagaa aatggattgt cttctaagat
     3721 agatagatat tctcttgtct gccaagcatt ttcgaatata cattccacat ataaaggctt
     3781 tttccaattc aaacggattt catgaaaaat ctggtctgtg tccgtaaatt cttttccctg
     3841 caaaaacaat ttcatgggtt ccccttcaac agatagatca ctgatctgca gtcctcgatc
     3901 cgcaaaattc gccccttcaa caaagtggac attgcttaaa atcgcttcat gattggcaag
     3961 ataattcaag atccaaaaaa cttctcttcg gctaaaagat acatgattga ccatccacgt
     4021 taaaaaattc ttcttttccc gtacatcaat tagcattttt ctcacctctc tgttaggtca
     4081 tctaataatg acagtgcctc catatctcct ggttcaagtg ttaaataata agtcaataat
     4141 tcttgcgctc ttttcagctg cccttcttct cttaagaaaa aggcatactc tttcaagaaa
     4201 tctggttcat gtgatagttc atgataagcc tgttcatagt gtacagctgc aacggtaaag
     4261 tcttctaatt cattataagc atgggctagg ttccattctg cataaggatt agcagtttct
     4321 tccatctgat tgatcacttg tatcacatct tcatatcttt catcatccaa gtaaagatta
     4381 cttaaagtaa gtaatgtttc atcttgtttg tctcctactt ctaaagcttt cagtaaaagt
     4441 tcttctgctc gctgtctatc atgcagacgg aaagcatttt ccgaggccag gtgataaagt
     4501 tcgacttggt aaggattttc tttgattcct tcttctaaca ctgtttgtgc ttcttcaatc
     4561 atttcttctt cttgaagagc ttgtcctaga tatagatata acgattgata atgcggatta
     4621 acttctcgaa gttcttccag atagttgatt gcctgctgat tttctttcaa ttgaagattc
     4681 gtaaaagcta gctggaacag tcgatcatct gtcctttctt ctttcagtgc ttcttctaaa
     4741 ggtacgatag ctgcttcgaa ttctccttgc atacttaagg attgtcctaa acgttcctgc
     4801 atcgagattc ctgaaatttc atttatcccg cttgccaaca atgtttcata aaccactgct
     4861 gcctctttga aacgatcaac tgaaaaatac aattcagcta gcgcaaactg gatcaatggt
     4921 tcatcaggaa gcaacccagc tgcttctttc aattttgctt cactcacttc agggattccg
     4981 ataacttggt acaaatcagc tatcgccaat aagctttgcg gatagtaatc actttgctta
     5041 tccacttttt ccaggtattc aaaagctaag tcgatctcat tatcttcgat tgcaatttct
     5101 gctaacggga tgttcagtcc gtcattttct gggtattggt tgatcagttg ttcaaaaatc
     5161 tgttttgctt cgatcaaaaa gccaatagcc agcaattctt cgcctagttc agctaatata
     5221 tctgctgggt cttcttttaa tgctttttct aaaaagattt gtgcttgtga caagtcttca
     5281 tctgataaag cttgcaacat tttttcgcta tttgtattca tttcaattcc ctcttatctc
     5341 actgacgatt tgttgaaata ccgtcattgt tctcaataat tctttttcgt ttggatgttg
     5401 gtatccgtct attataccta ttttttctaa aacaagtatt tttttgcttt tttctgccaa
     5461 aaaaagaact ttttccaaat actccatctt tgaaatctgt tccagccagt ggattggata
     5521 gccaagcaag tccagccaca caaaaaagtt ttttagttgg aaatcaaaag gatatgcttg
     5581 gagattccac acgaaatgaa acatcattcc taaaaatttt ttcatactag cagaaagtag
     5641 atggccattt tcggtcagat aaaaagcttg ttcaaacagt ttaccataat ttgacaggtt
     5701 ttctgcctct aattgataaa aatagatcat ttcttcgatc atcccggcaa acggccgagt
     5761 cgttaattgc tttttatttg gatagttttt atacagattt tgcaaaaaac gatgatcaca
     5821 aatcattccg caacagataa aagtcaaaaa gtcaatctgc tttccgtcca cctgccgctc
     5881 gctgattgtt tgatcataaa cgatgcgttc tggcagattg atggtttgca gcactacacg
     5941 atttggtagc tgcaaaatcg tccgctgcgg tgttaatgct cggcttagag aacggatcga
     6001 tacagggatc atccatagtg atgaatcaag ggttgtatgt ttttgaaaaa aacctgccag
     6061 atccatgatc ccttcatttc caaatccgat gataaacatg gattgatttt cgggatagcg
     6121 tttgacgaaa tcaagcaggt tctttaattc tgttaaatga ttgcattgag tgttggcaca
     6181 aatataccag tcattgtttg cttgatttgc aaataacggg ttgatcttat ctgcaaacaa
     6241 atcataatag cgctgattcg ttaaaaataa gattggctga tcaatggaaa gttctcttag
     6301 ctgactgact agcgtttctc cgtaaaacac tttcgtcaac gcatcttcta cttgataatc
     6361 aagttccata cgcatccctc ctcttcttat ttttatttta ccattagtaa gcaaattcta
     6421 gaaaagataa gagcaaagct cacctcataa cttggaaata aataaaaatt aaataaaata
     6481 catgtgtttt ctatgtatga catacggatt cggaatctct gtctcttatt tattcctttt
     6541 ctgctaggaa ataaaaagaa aagcacttgt agccctagag ccacaagcgc tttttcaact
     6601 gtcttattta acagcgtctt ttaatgcttt acctggtttg aatgcaggta ctttgctagc
     6661 tggaatttcg atttcttcac cagtttgtgg gttacgacct ttgcgagctg cgcgttcgcg
     6721 aacttcaaag ttaccaaaac cgattaattg aactttttcg ccgttagcta aagcatcttg
     6781 aatagttgaa aatacagcat ctactgctgc agttgcatct tttttagtta aatcagtagc
     6841 tgaagcaact ttttcgatta attctgcttt gtttgccatg gatagattca cctcctccac
     6901 aagagatatg atagaaatat catttagtta aaactttctg agtggtccag aagtttaaaa
     6961 agaaatattg attaacttca atattctgtt atcacgatat catatgaacc gcttaatagc
     7021 aaggttttta ccaattttca catgcttttt ttagtcatag aaacgtaaca aacgaaactg
     7081 tctttttctt ttgaaaaaaa aacgtcgata tggtaaaatg cctattttct cctacgaggg
     7141 ataattcgga tggacgtccc ttcaaaagta aatgcttttc tgatttgatt ttctaagaaa
     7201 cgttcataag aaaaatgcat caattcctct tcattgacaa aaacgacaaa tgtaggtggt
     7261 ttgacagcga cttgtgtagc ataaaaaatc ttcaggcgtt ttcctttatc tgttggtgtt
     7321 ggattgattg ctacagcatc cataatgata tcattcaaca cagccgaaga gatacgaaga
     7381 ttctggttca tacttacttg ttcgatcatt tcaggcagac gttccaaccg ttgtttagtc
     7441 actgcagaaa caaaaacgat tggagcgtaa tcaagatacc ggaattcatc acgaatctct
     7501 tgttcgaaat cacgcattgt attggtttct ttctttacaa gatcccattt attcacgacg
     7561 ataataattc cacgaccagc ttcatgggcg taacccgcta ctcgcttgtc ttgttcccga
     7621 atcccttctt cagcattcag taccatcaaa acgatatcag aacggtcaat ggcacgcatt
     7681 gcacgcatca cgctgtattt ttcagtattt tcgtatactt ttccgcgttt acgcatacca
     7741 gctgtatcta tcattaagaa tttctgacca ctttcagaaa cgaagtgggt gtcgattgcg
     7801 tctcgggtcg ttccttcaat atccgaaaca attacacgtt cttctcctaa aatcgcattg
     7861 atcaaagagg atttaccaac atttgggcga ccaatcaaac taaacttaat cgtgtcgtcg
     7921 tcctcttctt cagtctcttc agagaaatac ttcactgctt catctaaaat atcaccaatc
     7981 cctagtccat ggcttcctga aacaggataa ggatcgccta gccctaagga ataaaattca
     8041 tagatatcgt ttctcatttc tggattatct actttattga ctgctaaaat cactggttta
     8101 ttacttctgt ataaaatttt cgcaaccatc tcatctgcgt ctgtcacgcc ttcgcgtccg
     8161 ctgactacac aaatgattac atctgcttct tcaatcgcga tttctgcttg atgcttgatc
     8221 tgatccataa aaggttcatc gccaagatca attccgcctg tgtcgatcac gctgaattct
     8281 cgacctaacc attcaccttt tgcataaata cgatcacgag ttactccagg agtatcttcg
     8341 acaatcgaga tacgctcacc agcgatgcgg ttaaaaatcg tagacttacc gacgttcggg
     8401 cggccaacga ttgcaattgt tggatttgcc atattatttc ctcctcacaa gtttccaatc
     8461 tcacttagtt taacgaatgc attgccaact ttcaagcata gattgtcaat taaacgaagt
     8521 atttttattc cttattgaaa tactaatcgc ttttcaaaag aaaaactgag acaaaagcag
     8581 ctgctttcat ctcagtcaca gatacaaaat caattatttt tcagaatcat cggttgcttt
     8641 ttcttctact tcttctttca acgcatcacc taaaatgtct cccatagtaa atccagtatt
     8701 ttcttctggt aattcataag actcaacatt ttttggttct tcttctgaag ctggtttttc
     8761 ttctaaagct ttgatactca atgcaatacg atgttcttct ggatggatct ctaatacttt
     8821 cactttcact tcgtcacctt catgaagcac ttcatgtggt gtcgcaatat gtttatgtga
     8881 aatttgagag atatggacta gcccttctac tccagggaac acttcaacaa aggcaccgaa
     8941 gcttgtcaaa cgtttaactg ttccgtctaa tactgtgcca actggagctt ttgtatcgat
     9001 ttctgaccaa ggtccaggct gtgtgtcttt gattgacaag gaaacacgtt cttgttcagg
     9061 attgatagac aacactttga catttacatc atcattgaca gccaatacat cacttggttt
     9121 tgctacatga ctatgtgaaa tctctgagac atggactaga ccgtcaattc cgcctaggtc
     9181 gacaaatgcg ccaaaatcag taagacgtgc aacacgtcca ctgagaacat ctccctcatg
     9241 gatagttgaa agaatttctt ttttcttttc ttctctttcg ttttccacta ccgctttatg
     9301 cgataagatc aaacgatttt ctgatggttc gatttcgatg attttaaagc tcaatgtttt
     9361 gcctttatag tctgaaaagt ctgcaacaaa atgatcttcc accattgatg cagggacaaa
     9421 accacgtaca ccgacgtcaa cgactaaacc gcctttgaca acatttgtaa caggtgcttc
     9481 gatgaccttg ccctcttgga aatctctttc gatatcttcc caaacttttt tagcatctaa
     9541 acggcgtttt gaaagtaggt agctgccgtt ttctttatct ttgccgatcg tactgataac
     9601 gactaaatct aatacatcac cgacttttac tacttcgctg atatcttcta ccggcaaagt
     9661 tgataattct tttgccggaa cgactccttc aactccagcc ccttcgattc cgacaatcac
     9721 ttgtttgtct tcaatgacta aaacttcgcc cttaacaaca tcgccaactt ttacttcttg
     9781 tacgcttttc attgcgtctt ccattgactg gttgttctct tgattttgat caaattctgt
     9841 cataacactt gtcctcctaa ccaacattct gccgtaagta cggtactgtt tttagtttaa
     9901 ttgattattc aaaaaaaata aagcgatttc gttcgttttc cttgaaaaaa caattgtttt
     9961 tcattttttt tcttaattta aaaaagaaga taaccttttt tcaaaacaac agcttttatt
    10021 gctgcaacga cttctggaat cgatttgcca gtcgtatcga tacgaacagc atcctctgct
    10081 tgcttcagcg gagagacgtc ccttgttgaa tcataatggt ctctttcctg tatctctttc
    10141 gtcagtgttt caagatcagt cggaattcct ttttcttggt tctctttgaa acgtctttct
    10201 gcacgctcac ttacgcttgc tactaaaaag attttcactt ctgcattcgg taatacagct
    10261 gtcccgatgt ctcttccgtc catgacaacg ccaccgcgtt cccctatttt tcgttggaca
    10321 gcaactaatt cttctctgac ttttccatgt gcagagactt cagaaacagc ttttgtgaca
    10381 tctggttgac ggatttctaa agtcacatct tttgatgcta tgaatacatg ctgtccatct
    10441 tcttgttgcg aaaaagagat aggatacttt ttgatcagcg aaactagccc ttcttcatct
    10501 gaaaaagata cattgttcgt aatagctaaa taagttactg cgcgatacat ggcaccggta
    10561 tctgtataaa tataattcaa ttcttttgct aatatcttgg caacggtact tttcccagat
    10621 gaagcaggcc cgtcgattgc aatactgatt tgtgtcatga tataactcct ttttctcaga
    10681 aaaaaagccg caaagcgcgg ctattattat ttaattctta attcttgacc agggtaaagc
    10741 ataaagttgt ttggatctaa gccattcaat tcaaacaatt gatcagtggt gatccctgca
    10801 cgctcagcca cttgttttgg accttcgcct gcttgaacag tcgttgttga accggctgca
    10861 gctgatgaag aaggtgttgt ttctgctgca ttgctgcttg aatcccctgc ttgataagtt
    10921 gcctcacttg aggaagtcgg tgttgtctct gctgcaatac tgctttctga cgttacacct
    10981 gtatcttgag acacagtaga ttcttccgcg attgtactag attctactgt tgaagtctca
    11041 gttgactcct cttttgttgt acttgtagaa ctttctacta ctgatgtcga agtttcagaa
    11101 gaagttgctg ctgttgcatt gttgcttgat ccatttctga tccagaaata agcacctgct
    11161 ggaatagcga tacataagac cagtaaaatc aaaaatagcg aaagaaataa tgtatttcct
    11221 ttcttttgtt gtctctgaga acttcttgat gaaacttcat catctgtatc gtagattggt
    11281 tgttcccatg gttcttgtgt ttcgttattt tctgtagatt gatgtctatc ttttctgctc
    11341 actgttttac ctcctaaact ctatcgtgtg tattatacca taggaaaaag tgtcttgtcc
    11401 taaaaaatca atgttccatg ttttttttaa ataaatttag taaaatcgtt tcccaatgaa
    11461 gttgttcagt cttagaaaaa agcttcctct ttggcagaag ttctgctttt gctccatcaa
    11521 tatcacaaca attctgcggc ttttcggaga gtttttcgcc aaaataagct aagatgaatt
    11581 ttctgcggca gttttcttca ttaatatagc gaagcatctg ctgcaggcgg aattcttttt
    11641 ctttttcctg acgtttgagc cgttccaccc aattttctgg tttttcagac tgctggatca
    11701 gctccatcca ttttttttgc agctcatcaa agggagcttg ctttgtggca tattccaaat
    11761 aaagctcaaa actttgtcgc tgttctctag aaagttgatt aaagaaataa tggatacgtt
    11821 catccccact ttgataaaaa agaattgctg cactttcttc ctgatctctg cctgctcgac
    11881 cgatttcttg tacatagttt tctagactgt ctggcaaatc ataatggacc acataacgga
    11941 tatcagattt atcgatcccc ataccaaatg cattcgtagc aatgagaaac tgcagctgat
    12001 tttttacaaa ctgctgttga agttgtcttc tttgagctgc atcaagacca ccgtgatagt
    12061 atccaactgt aaaacgttcc ctgaataaat gatacaatcg ttcgacttct ttttttgttg
    12121 cacaataaat gattgccgct ccttgtgctc tttccatgaa gtgttcaagt tcttgttctt
    12181 tttgctgcgt tttccgaaca aaaagcgaga tattttgccg attgactgat tgtctgatct
    12241 cttttggtgg tcgatcaaat agtacatgac gtatatctct tgctactaaa tctgttgctg
    12301 tcgccgttag tgccagtgtg accgggtttc ctaaaagctt tcggattttt cctaactgtt
    12361 gatattctgg acggaaatca actccccatt gagacacgca atgagcctca tctactacat
    12421 ataacgcaat attttgtttt ttcagatgct ctaaaagaga aggattagtc aacatttcgg
    12481 gactcaaaaa caaaaattta tattgcgata gatgacggag gacgtatctt ctttcagatt
    12541 gcgaaagtaa actattgaaa gcagctactc gtttctcccc tcgtttttgt aattgcgtca
    12601 cttgatcttc cattagagaa agcagcggtg acacgatgat gaccagacct tccattaaat
    12661 accccgtcaa ttgatagcaa aggctttttc ccgttcctgt aggcaaaata gcgagagtat
    12721 cttctccatc aagcaacgcc tgaataattt cttcttggcc gggacgaaat gaatcaaatc
    12781 caaaatattt ttttaattca tttattaaca ttctgtatcg cttcctttct caatagatag
    12841 atttgataca aacgaaattc tccataatct aattgtccat ctacattcca ttcgctaaag
    12901 cgccactctc gcacggaatc cttatttggt tccaaacgtt cgattgtttc tgcagagaga
    12961 atccactcgt atggaaaatc atcaaaaaac aaggcccact caataagatg atctgttact
    13021 gttcctcttt ttaaatgacg gattgccatg atctggtcaa ttgtctgacc attcataaac
    13081 atctgcttgg tgatcatcat gctttgattg aaattttgct gtaataatga atcgatcaaa
    13141 gcgtgccaaa gcgaatctgg ccgtttttcg atttgtgcta acaaaaggtg gatacatctg
    13201 ctttgttgca gataaacctc tgactcatca ttgttttttg ccagctgata aggaagcagc
    13261 cctgttcgct catttccaga taattggtta gctaaaaaat ctgctgcttc ttctggtatc
    13321 tccgaaaaca gctgcaccaa actctcatac gcagtatcaa tcaaaatttc acgtggcaat
    13381 tggctttcac taagccattt ttttagttgg aaagtataaa aaggacttgt ctctgctgga
    13441 agatagtctt gaatacccgt tgccagatta gaaattactt gaacagcaaa tttaatgagc
    13501 cgccaactag tttcccctgt tctgccatag cggtcaaaac gcaaaccagt atgttctatt
    13561 tgatgttcgg acaacatatc agcccctgca ggcgttagct ttgcttcatt ttcatttatt
    13621 tcgatccaac cttgctgcgc aattttttgc aaaatttgat aaaacttatc ctgcttcaat
    13681 tctggaaaac tgttatggat aaacaaaagt tcgtgggtaa atccataaat aagtactgaa
    13741 gtcgttcgtt ttcccacaag gagttggtaa agagaactaa tccgcagctt gtgtcctgat
    13801 gaaaatagcg ataaaataaa aacaatcata ttattctcct taagttagga tggttagaaa
    13861 tgtcactact atgtaaactt gtacctgaac gctgtattgc atgcggtctt tgtcaaatcg
    13921 aagcgccaga tattttcgat tatgatgaag aaggaatcgt tttatttgcc caagaaccag
    13981 aggctcatca acagttcgtg atgccggaag aagaaacata tgtaaaaaaa gcgtatcaaa
    14041 aatgtccagt tcgagcgatt ttattagaaa ataaaatata aatataaacg aaactttaaa
    14101 aacgtacact aagttctcta aacttgaaaa taagccggga tctgtttctc ttgttccgtg
    14161 aaatcccgac ttattttgaa agattcattt gctatctata gttaattgac tagttttttc
    14221 acggacccta taggacccat ttatattatt tcaacttttt cagccagttt tccaatgtat
    14281 tgacgatcac agtttgctgg tctgcgttag aaatttctgc ctctccatct cctttttgct
    14341 caccatagct gccaaaacct ccatgatttc cgccattgat tgatacatac tctgttcttt
    14401 ccggtaaata ctgcttagct tcttgataag cttcctgatt caatacttta tcttttgtcg
    14461 ccgtgatcga taaaacagga acagctgatt ctgtcaatgt tccttttttg tcgggataac
    14521 ttgccatgaa gaatacccct tttaaatgtt tgtctgaatg attcttattg acataacgac
    14581 ttgccattac gccacctaat gaatggccac cgatcacata atcggaattt ggaaaatctt
    14641 ctttgacctt atccgctttg tttccgccca tcactgctag atcaagtggc attttgacaa
    14701 tagcgactga aaagccgttt tttgctaaat cttgcgccca aatactataa ctttctggct
    14761 ctacgagagc accaggataa aaaataagta atggagactt tccatgattc tttttctctc
    14821 cttcaaaaaa taagtaatct tctttgtttt ttccttcttt taccaacact tgcgcttctg
    14881 acgatggttc gtacgtattt tgctgtatat agtaataacc ggctccaccg agtaatccta
    14941 aaatgatgac taagccaatt agtattcgtc taaatatttt cactttctcc caccttttct
    15001 atctgagtga cccctttttc ttaatcaagt atgattatac cttaaaaaaa taagagagaa
    15061 atgcaaactt tccagcatca aataaaaaaa gacggaatat ccatttgaat atccgccctt
    15121 tttgtatgtt tatttttatt tagcacataa acgatacaca gcttctgcat aaatttccat
    15181 tgctttatac atactactta attcccatcg ttcattcgcc tggtgcataa agtcaggagt
    15241 atcagggaac atcgcaccga atgccacaca ctggttcatc gtacgagcaa aagtagcgcc
    15301 gcctgataca aacggttcag tcatgtctcc agtaatatcg cgatatgtcc ccaatagatt
    15361 tttcactaac tcactgtcta gtggcacgta taatgaatca aggaagtcaa attcttcata
    15421 agtcaaccca tatttttctg ctgttttagt taatttagct actaaatcat ctttcttgaa
    15481 tgtaacaggg attctcatat ctacaccgat ttttgtttgt tctggtgtga tttccaaact
    15541 tgcaaagttc atcgttagct cgccggattg ttcatcttct gttttgccca cgacagcttc
    15601 accagttgca ttttcttgaa ctaattttcc taagaaattg attgggccaa aatcaaatac
    15661 ttctgaaaga gcaattgcca gacgagaaat agcattcact ccttgtgctg catctttcgc
    15721 gtggatactt ttaccaagaa cgacgatgcc ttcaccttgt tcttcgaaat cgaattcgtg
    15781 ttttttcaat gcttctttta cttcagaaag cttttctcca gagtaaggag ctgcatcagg
    15841 aacaacattc aacgctcctc cagcttttac agaaaactcg ttcgttcctg gtcctgtcaa
    15901 gtaagcttgc aataagcctt tttctgcata aattaaagga aattcagcat ctggcgcaaa
    15961 tccttgcgta attccttctt ctttttcgtt atatttttct aaacaacgcc ataaattttc
    16021 ttcatctgta ccaaagataa aacgaatacg tgtcttgaat tccacgccag catcaatcaa
    16081 agctttgacg gcatacattg ccgcaataga aggacctttg tcatcttggg atccgcgacc
    16141 aactaaccag ccgtctttta tagttggatc gaatggtttt gtttcccaat tattttcgtc
    16201 ccctgctgga acaacgtcca tatgacacaa gataccgaat aattcatctc cagagccaat
    16261 ctctgaataa ccgtaatagc cttctggatc tttaaatgtt ctaaatccta gcttttcact
    16321 gatttccaat actttatcca aagcttcaat cactttttta ccgaagggat gtccttgtcc
    16381 tgaatctgct tcatctagta cggaaggaat tcggatcaat tcgctcaatg attctaatgc
    16441 ttgttcatga tgttgttctg tcacaaattt tttcatgata ttatcctcct aatatgttaa
    16501 aaagggtcat gtcaaattcc tccgacacaa cctcttcttc tcaaataatg acgttttaag
    16561 cctgctccat cagcaagcag aatgttctta acaaatgatg aaagaaaaaa cactcactga
    16621 atggagctat tgacttgtcc acgtgcttat cgatagccgt ttatcggttt cttcattctt
    16681 atgatagacc ttgtacctgt atttgtctag gtttagaaca ttgctgctac acctaggatc
    16741 acacagatgg ctacaaataa tatcgcaatt aatttaccgg taaatttcca ccacactgaa
    16801 acatcgaaac gagcaattgc tacagcaccc attacgatag cagatgttgg tgtaattaag
    16861 tttactaaac cagatgcaga ttgataagca gtgattacta aatccttacc gacgccggca
    16921 aattcaccca taggtcccat gattcccatt gttgcagcag ctaagccgga agttgaagga
    16981 atcaagaatg acatagggat atagaaaata aatgtaataa tgatgaatac acttgaagat
    17041 aatgaactta atccttgttc gccccaatgt aagatggtgt ctgtgattaa tccattgttc
    17101 ataaccactt ggataccacg agcaacagct actacgattg ctacaccgat caaatcagaa
    17161 gcaccagcta aaaattcaga gacaaattca gactctttca tgccataagt gattgctact
    17221 acgatggaca ttactaagaa taacatagta atttctggga agtaccagtc acctaatgga
    17281 agcatgcttt tacctagaac cgtacctagt accggaaggt ttgttaacca gtttgtcaat
    17341 gattcaaaaa tcgtgaaatt cgcattcaag gttgtccaag gaatcaaact gatgatcatg
    17401 ataccaaaag taataaagaa caatacatta acatgttttt gttttttcgt gattacttct
    17461 tgacgaccga ttgcttcaat atcgaaatgc ttcatatctt ctttacgttg gttgtatact
    17521 aatgattttg ttggatcttt ttcgatcttt gatgcataac gatacacata tacaatagaa
    17581 atagctaaca aaataacgaa catcaagaat cgaagaccaa taccttcacc catcgaaatc
    17641 ccaacagctt gtgaagctac gcctgttgca aaaggattta ccgttgaagc tagacagcct
    17701 acttgagacc ctactaatac gattgctaca gctactactg tatcaaagcc tacagccatc
    17761 atgactggga tcagtaaagc atagaatgga atcgtttctt ctgccattcc gtaagtagag
    17821 ccgcctaatg caaataacag catcaatact ggaataagca ttttttcttt tcctttgaat
    17881 ttcttcacaa ctgaagcaat cccggcatct agtgcccctg ttttgttgat aatacctaaa
    17941 aatccaccaa caaccaaaat gaagaaagag attgggattg cagcaggtgt ttctttagtt
    18001 agctcccccg ctggtacacc tagcatccca ttgattggcg ccatgaaaat atcccacagc
    18061 ccttgaggat tttgttgtac tcgttcatat gatccagcga cgatgttgcc tgcatcgtct
    18121 acactatagt gcccagctgg tataaaccaa gtaagtacag cgattactgc aataataata
    18181 aataagaccg tatacgctga aggcatttga aattttttct tttttgtttg ttccatcttc
    18241 ttcttccttt cttttccgtt agagacaaca attttggctc taatgttatt ttatcaaaaa
    18301 gagtatcaga acaaaaataa taacacgcat tttgtaaaac ttttgttcaa aaagttcgtt
    18361 tttccctatt ccctcctctt ttttcagttt tttaaagctt tcttcttttt cattgtagcg
    18421 tttccttttt tttataaaca aacattttcg tcttttaaaa ggagaagaat ttcacatttg
    18481 caaagaattg attgattttt atgcataaat caaaaaaata ctcaaaacaa acaaataaaa
    18541 cgtttacatt attgcatgtc ttgtattttt ttccaaaaaa aaagaccaag aaatttcttg
    18601 atctttttga tatttatttg actgctgtat gacgttgttc gaactgatgt tgttttcgac
    18661 ttaaccatgg aagaagttgc gcatgcagca ctaaaaagac agcactcacg atgaatcctt
    18721 tgatcaagtt gaaaggaacg atcccaatca gtacatagtt tgctaaagac atgcccagca
    18781 tctgtgttgc gttcagccct aaaaacatta agtaaagagg cgtaataacg aaataatttg
    18841 cgatactcat aaaaatagtt agagcgatgg ttcctgtaat gacacctaaa aatttgtttc
    18901 ttcctttatg ctgtttcttt acactaaaga aataataaat cgggaatgta aagatcgttg
    18961 ttgataagaa actagcagtg tctcctacca aattatccgg agaaaaacct gtagtcagca
    19021 gatgtaatac tgaacgcaag aacgctgttg caactccggc cgctggaccg aataagaaca
    19081 tgctgatcaa taccgagata tcactaaaat cgatcttcaa aaaactaaaa gccggcatga
    19141 tcggaaaagc aaaatactgt aaaactaatg cgatcgcagc aaacatcgct actgcaacca
    19201 ttttctgtac acgggtgttc ttcatttgaa atcctcctgt taggactcct cttcagcgaa
    19261 ctttttgacg tttctctcca aatgaaaaaa ctctattttc cagggaaaat agagttagag
    19321 ttatttgatc aagcgtcgtc aaataagctc tatcttcttt atccagacta tactgtcggt
    19381 atcggaattt caccgattca gctatttttt tgcaaaatag gtcgtggact ataaccaccg
    19441 gtcgggaatt tcaccctgcc cctgaagacg aaccttatat aatttttgtt tacatattca
    19501 ttatacatat ctattcaaaa aacgcaacat ctaacaatta taaaaagtaa gtgttcattt
    19561 tgagtggttc agcaaactgc tgacttcttt tttgttcagt tcacggtaat ccccaggacg
    19621 taggccttgt aaagtcagat ctccgtattt ttcacgtttt agtttttgca caggaagacc
    19681 aactgcttga agcatatttt tcacttgatg attacgtcct tcgtggatcg tcaattccac
    19741 gatactgtgg tttgtcttag gatccactga taaaatttgg aatttagctg gagatgtttt
    19801 tcgtccttcg atctttattc cttttgtcaa aggtgcaagc atcgattttg tagcgatccc
    19861 ttttaccttt gcaacgtaaa ctttatccac ttcatgtttt ggatgtgtca ggcgctgagc
    19921 aaaatctcca tcatttgtca gtagcaagat ccccgaagta tcgtaatcca gtcgacctac
    19981 aggatagata cgttctttta ccatcggcaa aagatccgta acaacttttc ttcctttgtc
    20041 atctgcaaca gcagaaataa ctcctttagg tttgtacaat aaatagtaac cgtattcttc
    20101 ttgataaatt gggacttcat ccacttccac agtgtcatgt ttcccaactt ttatccctaa
    20161 ttcccttaca acttgtccgt ttacttttac acgtcctgca aggatgaatt cttctgcttt
    20221 tcgacgagaa gtgatgccag cgtgggcaat tactttctgc aatctctcca ttacaattat
    20281 tctcctttat tcattccatc aaagaataga tctggtgaca cgtcttcttc aagtgaaatt
    20341 tccatttctt gtgtatcagg cagttcttct aatgatttca aaccaaaata atctaagaag
    20401 tagccagttg ttccatataa tatcggccga cctggtcctt ctactctacc tttttcttca
    20461 atcagtttat gggcagctag acgctgtacc gctcctgaaa cctgaactcc tcgaatctgc
    20521 tcgatttcag ctcttgtgat tggctgttta taagcgatga ttgctaaggt ttcgacggcg
    20581 gcttgagata atgtgtttga tgtcccttgc gcatatcgct taagtaaggg agccagctct
    20641 tttttagtcg ttaatacata atggctttcc gtttcaaaaa tatgcaaagc tgagtgcgag
    20701 ttttgagtgt aagattcttt taattctttc actaaacttg tgacttctgc ttcgctgaga
    20761 tttaacagtc cgcttaattc ttcattactg atcccttcct cgccaacaat aaaaagaagg
    20821 gcttcaagtt cacttaattt cgtcatgttc ggtatctcct actgctggat aaagcataat
    20881 tgattcatag ttttcttttt gttgcgcaat gatctgccct tttttcatca attctagcaa
    20941 agccataaaa gtagtgacaa cttcactttt agaaggtgac tcaaaaaaag aatcgaaagt
    21001 acagccatgc gcatttcttt ttatacgctc ttcgataaat accattcgtt cttcgatcgt
    21061 tgtgtcatcg ggcgtgatcg ttttttccac tttttgagaa aatttcttac gttctaagat
    21121 tttctgcatg gcacggatca aatcgatttt tttgacttgt cctcgcttca acggcttttc
    21181 agcatccgct aacccatcga tgttcatagg ttcttttgtg taataaagac ttcgttcctc
    21241 tgctttttct gtcagtcttt cagctgcata tttatattta cggtattcta aaagctgagt
    21301 gaccaattct tctcgaggat cttcttccca taattcttca tcgtctgtgc cgaattcttg
    21361 gacaggaagc aatgtcttgc ttttgattgc catcaatgta gcagccatca ctaagtattc
    21421 tcctgcaaca gctagctcga gtgttttcat tgtatggata taattcatat attgctccgt
    21481 gatcgtagcg atcggtatat catatatatc gatttccatt tgctgaatca gatgaagcag
    21541 cagatcgaga ggaccctcga atacatctaa tttaatattg atctccgtca actcattcac
    21601 tctttcttct ttttctttgc tagccatttt ccaatgtaag gctctgtgat ttcagttgca
    21661 cggatggaga catccgggta cttttcctgt aattcatcta acatttgaaa gcccatattc
    21721 tcaccgcgat aagacggatt caaagaaatg tcatggatga tcagctgatc gttcgttgcc
    21781 cagtaaatac caataatccc ttgaatgttg gttgaacctt ctggcgtaaa gaaaaaaagt
    21841 tcataatcgt cttcagattc gtacgtatca atctccttca acaaagaagt tttacctgtc
    21901 agtttcttat ggaaactcaa tagtcccata gcaattttac ggttttcttt ccggtaatgt
    21961 gttaacatct tctcaccttc ctatgactaa gcccttggaa aatgctgctt ataaacatcc
    22021 gtcattcttt tcttcgtgat atgtgtataa atctgagtcg tagagatatc cgcgtgacct
    22081 aacaattcct gtactgtccg caaatcagct ccattttcta gcagatgtgt ggcaaagcta
    22141 tgccgcagcg tatgtggtgt tacttctttg taaatcccag cctctcgtac taactgcttc
    22201 agatttttcc agattccttg ccgagaaaga cctttcccat gattattgac aaatacatgc
    22261 atttcagatg cgtcctttac aagtaatgga cgtgcttcgt ctaagtagcg ttccagccat
    22321 tggatcgcat aatcacctaa aggaatgatc cgctctttat ctccttttcc aactgtctgt
    22381 accagtccaa gagaaagatg aagatctccc aatttcaatc caatcaattc actgacgcgc
    22441 atcccagtag catacatgac ttccaagatt gcacgatctc ttatccctaa cgtttttgtc
    22501 gtatcaggtg cttcaatcag cttttctact tctgtcagag tcaatgtact tggtaatttc
    22561 tgtgctttct tcggcgtatc gatatgttgc atcggatcat gatcagtgat acgctcttgc
    22621 cgtaaaaact gatggaatct tctgagactt gagatcatcc gagtaacagt tgctgtcgct
    22681 tttttttctt catgcaactt ctccagaaaa cggatgacta aaaagcgatc gacatcctgc
    22741 catgaagcaa ttttctgctc ctgtagaaaa tgcaagtact gttccaaatc acgttcatag
    22801 ctttgtcgtg tattggcgga caatccccgt tcaatattca agtagtggag ataatcgttg
    22861 atttgctctt tcataataat ccccctcatg cctctctatc atatcagata ccgcttcatt
    22921 ctgatagtct ttttccataa tctgagcaaa gaaaaagccg tctagacact cagacggact
    22981 ggtttctttt ttcggctcta taatttatgg gactgatgaa tcagaattga ttcttcagcc
    23041 ccattttcat tttaggta
//
LOCUS       DACDXO010000043        22458 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E23605_2017
            SAMN12639989-rid9881463.denovo.043, whole genome shotgun sequence.
ACCESSION   DACDXO010000043 DACDXO010000000
VERSION     DACDXO010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639989
            Sequence Read Archive: SRR10023389
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741914.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:42:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,972
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 33 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 17 of 73
            Pseudo Genes (multiple problems)  :: 10 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22458
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639989-rid9881463.denovo.043"
                     /isolate="E23605_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..152
                     /locus_tag="IWM35_001827"
     CDS             <1..152
                     /locus_tag="IWM35_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9919676.1"
                     /translation="NFAKNQGYNTILLGAQSTAETFYEKLGYTAYGDPFEDAGMPHIY
                     MKKTL"
     gene            complement(230..763)
                     /locus_tag="IWM35_001828"
     CDS             complement(230..763)
                     /locus_tag="IWM35_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HAP9919677.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(857..2035)
                     /gene="mutY"
                     /locus_tag="IWM35_001829"
     CDS             complement(857..2035)
                     /gene="mutY"
                     /locus_tag="IWM35_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HAP9919678.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(2028..2825)
                     /gene="recX"
                     /locus_tag="IWM35_001830"
     CDS             complement(2028..2825)
                     /gene="recX"
                     /locus_tag="IWM35_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HAP9919679.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNHGKSQKELLRKIQQTLMTKGFSQEVIQQAIADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            2932..4314
                     /gene="rlmD"
                     /locus_tag="IWM35_001831"
     CDS             2932..4314
                     /gene="rlmD"
                     /locus_tag="IWM35_001831"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HAP9919680.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMIREINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(4488..5066)
                     /locus_tag="IWM35_001832"
     CDS             complement(4488..5066)
                     /locus_tag="IWM35_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin-binding protein"
                     /protein_id="HAP9919681.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
     gene            5388..5858
                     /locus_tag="IWM35_001833"
     CDS             5388..5858
                     /locus_tag="IWM35_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HAP9919682.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGKYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLTMWLMIPYYLWVLFATLLNYSIYFLN"
     gene            complement(5966..7192)
                     /locus_tag="IWM35_001834"
     CDS             complement(5966..7192)
                     /locus_tag="IWM35_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytR family transcriptional regulator"
                     /protein_id="HAP9919683.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
     gene            complement(7946..8146)
                     /locus_tag="IWM35_001835"
     CDS             complement(7946..8146)
                     /locus_tag="IWM35_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP9919684.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
     gene            complement(8280..8888)
                     /locus_tag="IWM35_001836"
     CDS             complement(8280..8888)
                     /locus_tag="IWM35_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HAP9919685.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNSAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            complement(8973..9443)
                     /locus_tag="IWM35_001837"
     CDS             complement(8973..9443)
                     /locus_tag="IWM35_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HAP9919686.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            complement(9491..10405)
                     /locus_tag="IWM35_001838"
     CDS             complement(9491..10405)
                     /locus_tag="IWM35_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HAP9919687.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKDVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLKLKY
                     GITHVTIQAETKAKDQKKLIFLNKDESY"
     gene            10551..11354
                     /locus_tag="IWM35_001839"
     CDS             10551..11354
                     /locus_tag="IWM35_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HAP9919688.1"
                     /translation="MTTKQLIISSFIRFKDLKEARNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKQLR
                     SFLSEASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            11532..12479
                     /locus_tag="IWM35_001840"
     CDS             11532..12479
                     /locus_tag="IWM35_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HAP9919689.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
     gene            complement(12547..12840)
                     /locus_tag="IWM35_001841"
     CDS             complement(12547..12840)
                     /locus_tag="IWM35_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis protein"
                     /protein_id="HAP9919690.1"
                     /translation="MKLTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEMDGTLFFIEEADEWFFKGYDLLVDYDPNLEEPTYHFKKQ"
     gene            complement(12868..13533)
                     /locus_tag="IWM35_001842"
     CDS             complement(12868..13533)
                     /locus_tag="IWM35_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HAP9919691.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            complement(13673..14305)
                     /locus_tag="IWM35_001843"
     CDS             complement(13673..14305)
                     /locus_tag="IWM35_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9919692.1"
                     /translation="MIKVLLVDDHEMVRLGVSSYLSIQEDIEVIGEAENGRQGYEKAM
                     TLRPDVILMDLVMEEMDGIESTKAILKDWPEAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNEPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            complement(14312..15379)
                     /locus_tag="IWM35_001844"
     CDS             complement(14312..15379)
                     /locus_tag="IWM35_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP9919693.1"
                     /translation="MMGKISRAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLLAISLGVGLIVFLLLSLIQKTKYGKIEEKLRALSSGNYESKLLLLP
                     IPSASDDLYIKDIDKEITKIKEKMIEVSSELQIVTSRPQYVDGQTKEEILELERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWDVEDVKLSSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVEIKVPLMKEA"
     gene            complement(15376..16107)
                     /locus_tag="IWM35_001845"
     CDS             complement(15376..16107)
                     /locus_tag="IWM35_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HAP9919694.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKHPRTKFQNFQLILGSLVIFFSLVNSPALWMMAVFAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMIQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAVISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKIYSS
                     DYDENPRRLKIITNTLIGDVEVIRI"
     gene            complement(16276..16755)
                     /gene="greA"
                     /locus_tag="IWM35_001846"
     CDS             complement(16276..16755)
                     /gene="greA"
                     /locus_tag="IWM35_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HAP9919695.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGE
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            complement(16891..18066)
                     /gene="mltG"
                     /locus_tag="IWM35_001847"
     CDS             complement(16891..18066)
                     /gene="mltG"
                     /locus_tag="IWM35_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HAP9919696.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPSGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELHQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEEAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVEKYVNNS"
     gene            18270..19439
                     /locus_tag="IWM35_001848"
     CDS             18270..19439
                     /locus_tag="IWM35_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HAP9919697.1"
                     /translation="MKKGKHAKKRKYSFLMIVVVLFSLSAFSTGTILGWQFYNNEHSQ
                     IVAHAQSAQKKLILTTNEQQLVEKIDQLLRKNDYIGSIYVKKNNRIIMEKGYGYANKL
                     TQQPNDPSLYYQIGSVQKALTTLLILKQVERGHLSLDTKLAQFYPQIAGSQQITIRDL
                     LYMRSGLHRTASPTIPMTDEEVVQFAIQHLKFTDYQTSHYDSLNFTILTGILIKLTNQ
                     PYEKLLTDDIIEPLGLQHTKFYDQVKNSSNHALSYQMSKEDDYFKPLTESETEIHNEL
                     GTGNISMSVYDLNTLFTQVLSGKLVSKDLLFSLWRENANGHPYSGGVYSGDNYILSQG
                     NIDSFDTSAIFSKDSQDAIIMESNIKADKTIKLPTADLRNQIYDLMEGTTKLANS"
     gene            complement(19706..21328)
                     /locus_tag="IWM35_001849"
     CDS             complement(19706..21328)
                     /locus_tag="IWM35_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9919698.1"
                     /translation="MNLKKIFSVKIFIVYAFVSLLYLFPAFKNMEIFGFYDTAFHINR
                     ALSLESIFSSPINFETFRSYGMQVNNFYPWLTLYPLFLLIKFTNLAIGYNLFLYIVTL
                     ITLFICHYVMYEITKKHVTSSFFAIIYTTSSFRSVEIFLRGAMGELLAMSILPLILLG
                     FIKLYDSKKESWVMLAISMTLLIYTHVLSVAMTMVMIIIALIIQFYRKKKIEIFLIIK
                     LIKAAIVTLLLSLAFIGPMIEQTLYQDLNRPYVDILQKRAIYLGKEFLIGSIDSELLS
                     FGIGLTLLICLIILTFNFKKLGILSKITYIMGIISILLCTKIFPWFALQNTPLNIIQA
                     PWRFMIFSTLFISFSVSLFISPQLEKFSYTRRRNFILLLILAISILNYSSVVNMVKEM
                     NFQGTYSNEQMENKLEEFENFDYVPIGYKKDQLLLKNHQVTEDGQVIYSSFSPNKDEI
                     TFTFKSSKGKTIVLPVFRYKGLTVTVNSKNATVLKQGGPFVTVKSESGINKVTIRYSY
                     TFFSKISLFISIITLIVLLFYCIKLKIRTSHE"
     gene            21825..22458
                     /locus_tag="IWM35_001850"
                     /pseudo
     CDS             21825..22458
                     /locus_tag="IWM35_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
BASE COUNT         6971 a         4369 c         3556 g         7562 t
ORIGIN      
        1 aaaactttgc taagaatcaa ggctataata cgattttatt aggggcacag tccacagctg
       61 aaacttttta cgaaaaacta ggatatacag cttacggcga tccttttgaa gatgctggca
      121 tgcctcatat ttatatgaaa aagactttat aaaaatttat ttcgaaaaaa gcaaaaaaca
      181 gcaagctttt gagaaaaatc tctcactggc ttactgttct tttttgtgtt tacttacgcg
      241 acaattgctt gtaacggtta taccaaatat caatatagcc ttccgagaac gggccatctc
      301 cattattgat ccagtcaacc agaatcttca cattttcttt taggatgaag tcaatatcgt
      361 tcgggtaatt catcattttg ctgtgcatct catattcatc gacatccaat agtcgtttct
      421 cgccgtcagg aaataccttg atatccaagt catagtcgat atatttcaag gcctcatcgt
      481 ccagcaaaaa aggtgaagct agattacaat aataggaaac ccccttttct ctgatcattg
      541 ctattacatt gaaccagtat tttttatgaa aataaacaat cgctggttcg cgcgttaccc
      601 atcggcgtcc atcagattca gtgactaatg tgtgatcatt cacgccgatc atagagtgtt
      661 cgcttgtttt taataccatg gtatctcgcc acgttcgatg caatttgccg tcgtgcttgt
      721 aactttggat cgtcacaaac tctccttctt ttggaactcc catagcaacc cttccttaag
      781 cagcattttg tgctttgtgt ttgggtgttc attacaccga catcataatt attatatcaa
      841 taaaaatgag ctttgcctag aaaggattcc tattgtttcg atttttctct aactgatcta
      901 ccagtttcat ttggacctta ggaaaaacga ccgaatcaaa tgcatcaggc ttcaaccact
      961 tcgaagtttt gttctcggta aattcttgtt ctgctccctc ggttgcccgt ccgtaaaaaa
     1021 ggagaacatg ccatttcaga tgactaaaaa tatgagtaac ttctcctaaa tgtcgtgtct
     1081 gccaaacaaa gagttcctgc ttttcaaatg ggagattttg ctcttcttct gctactaggt
     1141 catcaaacag atcgatttct ggctttgttt cccattcttt tttcaatcgc tcatactcct
     1201 cttgtgtcac ttccatcatt ggaaatgtcc acatattcgc cagcaatttt tggctatctc
     1261 tttcttcaaa ataatacgct ccagaatgat tttgaagagc tgcactgata taataaacgt
     1321 cttttggctt tgcttttttt gtttttacag gaaaactcgt ttggattcct cgtttgttag
     1381 ctaaacaaaa ggcttggatc ggacaagtct cacactttgg agaagttggt gtacagatag
     1441 ctgaacctag atccatcatt gcttggttga attcacctgg atgcttttca tcaatgattt
     1501 tccgcatcgc ttcatcaaag attttcctgc tagaagcttt tgcgatgtct gcttcaatgc
     1561 aaaacaatct gctgactact cgcatcacgt tgccgtcaac tgcaggttct ggaagtccaa
     1621 atgcgatact tgcaatcgcg cctgtcgtat atggtcctat tcccttcaat gagctaattt
     1681 cttcaggcgt ttgcggcatt tccccatcaa attctgacat gatctgttta gcagcagctt
     1741 ggatgttccg ggctcttgag taataaccaa ggccctccca tgcttttaaa agcttttctt
     1801 ctggagcatt tgctagctct tcaattgtag gaaaccattc catgaaacga taaaaataat
     1861 cgatcactgt gtctactctg gtttgctgaa gcatgatctc agagatccag atacgataag
     1921 gatcacgatt atagcgccaa ggcaggtttc ttttttcttg ttcataccat tgaatgaact
     1981 gatcttgaaa ttcttttgtt tctttatctg tccatttttc ccattcttta ttcactttcc
     2041 tcttcctttt ccgtaatgaa tcgctggatc atatccaaat caaatccttt ttgatataag
     2101 ctttgcttta cttttagatt tcttttttta ggttcaaacc ttaaatttct tcgccatagt
     2161 ttatctcctt gtttgactaa ataatcatat tctatttctt gatctgcttc ttgcggaaga
     2221 tcagctatcg cttgttggat cacttcttga gaaaagcctt tcgtcatcaa tgtttgttgg
     2281 atttttctca ataattcttt ttggcttttt ccgtgatttt ttccaaacgc tttttcggct
     2341 gtctgatgtg cattttgaac ttggtcttca aatgtatatt ggctgagcgc ttgttcaatg
     2401 atttcaggtt tcaaaccttt ttgctgcatc tgctgcgcta atttctttgg tcctttgtca
     2461 cttaagcgca tattcgtgcg cacataactt tccccataga ccaaatcatc gacaaggtcc
     2521 aattctttta agcgggctac gattttattt cgatcttctt gaaggatttc cttttctttt
     2581 aagtatgttc ggatttcttt ttccgatcgc aactgataac ttaagtaatt cattgctagg
     2641 tgaaaaccga aatcagctcc gctactcttc ttgatctctt ggatagtttc ttcgtctaat
     2701 tcccgtcctt ttaataatcg atagcgtact aatacatctt cagatacttg taaagcttct
     2761 ccttcagata aatctacgcg atagaaaggg cctttgcctt tactgattct cgtaacggtt
     2821 aacataaaca tcctcctcga acatatattc tctatcatac cacagaaaat ccccgtaatc
     2881 aggtattata tgttagaata aaccacagaa aaaagaaaaa gggagatact tatggcagaa
     2941 acaattgtca aaactggaca aacggttcct ttgaaaatca agcgtttagg cataaatggt
     3001 gaaggaatcg gttatttcaa acggttgatc atatttgttc catatgcact accaaaagaa
     3061 gaagtactag tgaaaatcac gaaagcaact cctcgctatg cagaaggaga attaataaaa
     3121 gtcaaaaaaa caagcaaaga tcgcgtagtg gcaccatgtc ctgtttacta tgaatgcggt
     3181 ggctgtcaat tacaacactt agcttatcaa gctcaattgg attttaaaaa ggatctgctt
     3241 ttgcaagcat tggaaaaatt caaaccagca ggctttcgat cttatcaact tttgccgacg
     3301 atcgggatgg atgagccttg gcattataga aacaaagcac aatttcagtt acgaaaaaat
     3361 aagcagactc aaaaaattga agctggttta tacgaagcaa actctcatca gttagtccct
     3421 ataacagact gcttggttca agaacctcga acacaaaaag taatgaatac tgttgttcaa
     3481 ctattgaata aatttgatgc acccatctat gatgaacgga caaacagtgg aatcttccgc
     3541 acaatcatgg ttagaatcgg tgttaaaact ggggagttac aagttgtttt tattactcgc
     3601 agtaaaaaat tcccgcaaaa aaacgcaatg atccgtgaga tcaatgaacg actgcccgaa
     3661 gttgtttcaa tcatgcaaaa tgtccaggca caaaaaacgt ctctcgtgat gggggatgaa
     3721 acgatccact tatggggaaa agaagccatt gaagaacaaa tcaatgaagt gactttcgat
     3781 ctttctcctc gtgccttctt tcaactcaat cctcagcaga ccgaattgct ttatggcgaa
     3841 ggaatcaaag cattagatgt ccagcctaat gaaacgatcg tggatgctta ttgcggagtt
     3901 gggacgatcg gcttaagtgt agcaaaacaa gccaaagaag tacggggaat ggataccgtt
     3961 cctcaagcaa tcgctgatgc aaaaaaaaat gcggaaagat taggatatca caacactcga
     4021 tatgaagtcg ggacagcaga gcaagttctg cctaaatggt tgaatgaagg ttttcagcct
     4081 gatggtatca tcgtagatcc accacgtacg ggtttggata acgcattgat ccaatcatta
     4141 ttgaaatacc ctccaaaaaa acttgtttat atctcctgta atgtttctac acttgccaga
     4201 gatctggtta atttaagtaa aaagttcaag attgaatatt tacagtccgt cgacatgttt
     4261 cctcagactg cacgttgtga agttgtcgtg aagatgacga gactcgctga ctaaatgaaa
     4321 gaggttgtga aaaagtctgc cctgtatcaa gtaataagaa cagccaaaca aaaatagctc
     4381 ttcatgtttt atgaatactg tacttgtgac cgaagagtta acaggaggac actatttatt
     4441 taaaaaaagg gttgtgacat agtttttgtc acagcccttt ttgattatta ttggatattc
     4501 acgtctatca cttgataaaa tgcatttgtc gtatcgctga tattccatac tgctaaaatc
     4561 acattatatc cttttcgatc ctgcggaagt gtgatcgttt ggttgactgt ttttggtgga
     4621 acagttgctt tgtcgtctat ttttgtgatc aattcaaaat cactaaattt caaaggttga
     4681 ttaggattcc aagttggttt ggtaatgtag taatcccaag tacttgtccg atgttgtgct
     4741 gttaaattcc aagtaatatt taatgttcct ggattaacca tgtttttatg ccatctgtta
     4801 gatgtctgtt catctaaagg ctcaaagccg ctgactcctg cactagcaat ttttccatca
     4861 atgaatgtat ttttggtagc ttcgatactt tgcggttccc attgtgctct tccgacattt
     4921 tgatttaagt ttcctgctgc tgatgttcct aggtatgcac ggcttcctgg agaagtaata
     4981 taaccatgcg caaaacctgg aatgctaaaa ccgaacaacc ctaaactaac taacccaata
     5041 aacaataacg tactaatctt tttcatgtag aaacctcctt catttgataa ccacattgta
     5101 aacgctttat tgtattaaca caaacattat gcttttataa ttttatgttg aagtaaaaaa
     5161 ggtgaaaaaa gtaagatgca gattataaca gaagtagacg agaaaagtac aaatagaaaa
     5221 catgagaatc aaatcatatg atctttttca aaaacatcat tcatataaac aaatgttcaa
     5281 ctttttaata acaacaatta tcaatttttt tcttacttca caaactaaat acaggtcttg
     5341 ctgtgagcct tcatggtaaa ctaatattat agaaaggagt tagtaaaatg aagatgataa
     5401 aagatattcg tttttgggtt tgtgttatag gaattgtcat attaggcttt ttatcagggt
     5461 tgcttagcgg aaatcccgga aaatattact attctttgca acttccacca tttgcaccac
     5521 ctagctggat atttgggccg atgtggacct tactgtatat attgatgggt atttctcttt
     5581 atttgttact taatcacaac aacaaaaaac agcgaaacaa tcttgtcggt ctttttgtga
     5641 ttcagttcat ttttaatttt atatggtctg ctctattttt taatttaaga aatattttca
     5701 ttgcagcaat cgatattaca cttttagtta ttttcttgtc agtgcttatg tatcagttgt
     5761 ggctgcatca tcgcctgaca atgtggctca tgattcctta ttatttatgg gtactattcg
     5821 caactctatt gaattattca atttattttt taaactaagc gctaaaaaga gacgtcctgt
     5881 aatttaacac atagaaaaag tgccacctaa gttagatttt ttgtctaatt taggtggcac
     5941 tctttattaa ccaaactatt tttttctagt acccacttgt tgttccatac tcattattct
     6001 ggtcataacc ataaccattt tgttcctgtc cagcgtaagg atcataactt tgagtatctt
     6061 gacttggtgt atagtactga cttgtatctt ctgtttgctg ataataggta ttcggatctt
     6121 ggtactgatc atatgtgttt tctgaatagg aagaataatc ggtgtaccct tcactattcg
     6181 atccatcacc gtttgtcgaa tcgccatctt ccgaatcatc aaagaacatc attgaagcat
     6241 tctcattttt caaattaggt aatgtatcgc ttgtttttat atttaattgt ttcttcagtt
     6301 cattttggat cgaaagaaga tcattttttc ctaaaatttg atagtagata tcattgatca
     6361 tttgtccttc gccttgcaac tggtcttgtt tgattgtttc aaacgccggg gtgtagttcg
     6421 tcgctacatc aagcatgtca tcccaatcta agtccgtcgt cacattgttc tcaactgctt
     6481 ttaaaatttt tctataattg cttacgccat ctaaacttaa aactttattg acgattttcg
     6541 tcactacttc ccgctgacga cgttgtcgac cgatatcgcc ttctggatct tcgtgtcgca
     6601 ttcgagaata ggctaaacct ttttctccat tcaatgtttg ttcacctact ggtacatgga
     6661 caccatccaa agtaaagtca attttattat tcactttgat gccaccgacc gcattgatca
     6721 aatcttccat gccatccaaa ttgatcgtta cataatgatc aataggaata tcgagcagtt
     6781 gctcaattga atccatggcc atttcgacac caccaaatgc gtaggcatga tttaatttat
     6841 ctaccgttcc ataacctacg atattcgtat aaatatctcg gtccaaactg atgatcgttg
     6901 attttttttg tttcggatta actgttacta ccatcatcgt atcagaacgt ccttgttccg
     6961 ttcttcctaa tccaccggta tctaaaccta atagcaaaac cgaaaaaggt tctttgtcat
     7021 caatgcttac tctctcagaa cgtttggagg atattcgatt agagttttta gaaattttgt
     7081 ttactgtttg gctcgcttct cccataaatc tgattccgta tgctgataat ccggcaatca
     7141 gcaaaacgaa tacccctaac aaggttaata gaactttttg ccatcgcttc ataatacacc
     7201 tctgtttcac attttctaaa atattgtctc atataactgt tcaatcagca aattcacttt
     7261 ttaatcataa gaaaacttta aaaaacattt ttgtgtaacg attattattt tttaaaattt
     7321 ctcaaaattc ttaactatca taccataccg tgaaaaagat cgaaagaccg aatacacgca
     7381 gttcgcaatc gttttatctt tttttatgta ttttttccag aaaaagcgaa caacaaggta
     7441 ttttccaaat aatcgtgcag taaaatgatc gcacatacct ttcccatctt tttaacagtc
     7501 tttgcctacc gttcagatta tcattcgatt tcacgacaat ttcttcctat gactggtcct
     7561 tttgttaaac ccatcttcta ttcgttttat ttccagctca ctgcatggct cttaaaatga
     7621 ctcgaactag tgattgcatt accatcatct tttaatggat agatatgctc ctcttcaaag
     7681 taaaacgatt ttcttttaaa gtaatagaat tgacatggta atgagtatgt atactagtgg
     7741 tttcacaaat ggtcttttat tcaacatcca aaaactgaaa tttctttaaa atccattact
     7801 aacgaagaaa atgttccttg cttgataatt agttaggaca tgtcaagtga acaaatgcat
     7861 ttacttagcc aaaaaatcta aaaacttcat aaatagacaa aaagacacga ctctctaaga
     7921 agtcgtgtct ttattcaaca aattattata atttagtcac ttcagcagct tgaggtccgc
     7981 gtgcaccttc aacaatcgtg aattctactt cttgaccttc ttctaggctc ttgaagccgt
     8041 ctccttgaat tgctgagaaa tgaacaaata cgtcatctcc gccgtctact gtaataaacc
     8101 caaatccttt ttcattgttg aaccatttaa ccgttccgtg ttccataaat gtatatcctc
     8161 caaaaaagtt cttgcaagta accttgcaag aacattatac ccaatttaga aaacgctagc
     8221 aagctttcgc tcttttattt tccaaattat ttttaagact gatatgacgg aggaaatggt
     8281 tattttgctg cttcgaaacg tttgtttact tcatcccaat taataacatt ccagaatgct
     8341 gcaatataat ctggtcggac atttttatat ttcaaataat aagcatgttc ccaaacgtcc
     8401 aaacctagaa ttggtgtctt tccttccatc aatggagaat cttgatttgc agtagaggta
     8461 atagctaatt ttccattttc cattacaagc caagcccatc cagaaccaaa tcgtccagcg
     8521 gctgcttttt tgaattcttc tttaaaagaa gaaaaatcac caaaagcttc attaatcgct
     8581 tcttttattt ctcctgtagg ttcgccacca gcatttggtg ccatgatttc ccagaaaaat
     8641 gaatggttag catgtccgcc accattgtta cgtacagctg tcttgatatc tgttggaata
     8701 gcgtccatat cagataataa ttcttctatt gttttttcgc ctaattctgg gtatttctca
     8761 attgctgaat ttaaattcgt cacataagta ttgtgatgct tatcatgatg cagatgcatc
     8821 gtttcttcgt caatataagg ttccaatgcg tcatatgcat aaggtagatc tggtaatgtg
     8881 taagtcataa aaaaattcct cctcaatgtt ttcttaataa tgtctacatt acaaagagta
     8941 acaaagagaa ggaaattctt caattgttat gcctagtcgt ctttttcact ttgacctact
     9001 tcttcttcaa aaacctcagt ttctttaaaa ataaacaact tgctgaaaat atagttcgca
     9061 aggataacaa taatttgagt gatgattttc gccacatact cattgctgtt cataacattg
     9121 atcaacaata ccattgcccc catgtccaat ccgtaagaaa ggatacggta gaagaagaac
     9181 ttaccgcatt caataagtgc atgtgtaaaa ttcggtgatt ttgaatggaa tacccaaagt
     9241 ttgttcgtta caaaagcaaa caatacagaa caaaaccacg aaatagaatt ggataaggcc
     9301 atacttaaat caaaatattg gcgtgcgaga gcaaaactaa caaaatttac aatcgtagcc
     9361 aaaccaccaa aaaagagata gactagtatc tcccaatatc cttttgcttc taaatatcct
     9421 tttaactgtc gatactgctt catgagccaa cctaccttat taaaaattct tatttatcat
     9481 actctatatt tcaataactt tcatctttat ttaaaaaaat taattttttt tgatcctttg
     9541 cctttgtctc tgcttggatc gtcacatgtg taataccata ttttaatttt aataaagaaa
     9601 ttatacgagg atatatcttt tcaatctcgc ttagtagctg atcttcaaaa tcaatatgtc
     9661 cgtcgaaaat aattatcttt tcgctatact gccaaatatg gacatgatga acatccttta
     9721 ccccatcgat tgcttcaatt tcttgttcca ttttttcata gtcgatagca ggtgcacttt
     9781 gtatcaaaat agcggcagct tttttgatca ccattatcgc ttctatcaat atatatatcg
     9841 aaatgatcag tgtaataact ggatcgatcc agtagatatc gaaaaaacgg atcaaaattg
     9901 ctccgataat cacacttacc gaagacagtg tatcacttaa caaatgcaaa taactagatt
     9961 ttatattcaa actttcatct gcttcttgtc gtaataataa tactgagaac aaattcgcga
    10021 gcaatccaat aactgctact gttagcatca aatgactatt tatcttttct ggtgaagtga
    10081 accgcttgaa tgcttcaacg gttaaaaaca aggataatat gatcaatatg aatgagttga
    10141 tgaaagcact caatatttct aaacgctggt agccatacgt acgtttttta tttgcttctt
    10201 ttcgggccat tttttgtgcc ccgtaagaaa aaataattgc caatgtatca ctaaggttat
    10261 ggaaagcatc tgaaacaaga gacaaactcc ctgacataat ccctccaata aattctgata
    10321 aagtgatcac cgcattcaaa accactactc caaatagttt ttttgatgat ctctgtggct
    10381 gtgtaggcac tattccttcc tccattttat ttatattgga tagtctttag acaagaataa
    10441 catttttttg atatttcacc aatcattcag ctgtttcctt aaagcacttg tatttcactt
    10501 gatacttcgc tagaattgca agtgatgaca ctactagatg aggagtactt atgacaacaa
    10561 aacaattaat tatcagttcc tttatacgat tcaaagacct aaaggaggcg agaaatgtcc
    10621 cattttggaa agccattgtc tatcttttag ttttgagtat aattatggct ttgccaatct
    10681 cttttcaagt ttttcaagtc ttaaacaaca ttcaattgga tgggcaaaag attgctactc
    10741 gcattcctga ttttacaatc caaaacgggc agcttcaaac cgaagaaaaa gaaggattca
    10801 tttaccaaac aaactccatt atcttcacat ttgatccaga gggaaaacga acagaacagg
    10861 acatctctac tgatttgatg gggaattttg ttagtgtcgg gatgttaaaa aacaaactta
    10921 tcatagctct tcctaataca ggcacgactt ctgctttgtt gaacaataac caattagaac
    10981 ttccctacac caatgaatct ttaaaaaact taacaggtaa gcagctccgt tcttttttaa
    11041 gtgaagcatc tataccactt tggataaaag tcctgacatt tatattttct atttatccta
    11101 gttttctgaa tctgctgatc actttgctat ttaccaacat tgctgctttt ctctatgcac
    11161 gctttcgact aacaaaagct acttttcttg attgtttaaa aacattgatc tattcaattt
    11221 cattgcctac tataattgca acaatcttga tggtctttct tccttctttt gacactagcg
    11281 ccttcattgc ttttgcagga atctttattt ttgctcaagc tgtcaaagga tgggcaaaaa
    11341 tatcgatcaa ttaagaaata aagacaactc gttatttcta cttcaataga cccttttaga
    11401 ggttgtgaca tttttttgtc acagcccctt ttttatttga aaaatatttc tttctcaaaa
    11461 aacaagcaag gcgcttcaaa gttcttcttg tatgcgctac acttagagta aagaaaggcg
    11521 ggaaattaac tatgtcagaa agaatcattt atgcagatcg tccatttcgt gatgaattta
    11581 tcgaagaaat ccatactata gcgcctggtt atatctttaa aacagaatta caaccagagg
    11641 atctacaaaa tgtagagatc agtttgggct ggaactcaga ctaccaagaa aaactattag
    11701 cctcttctac cttgaaatgg gtccaatcta tctcagctgg tgttgatcag ctaccccttg
    11761 agactttttc aaagaaacat attttacttt caaatggtag tggcatccac agtgaatcga
    11821 ttacggagca tgtcttagga atcatccttg ggtattctcg tggtcttttt caagcacaaa
    11881 aggcacagag tgacatgaaa tggttaggtt catcggtcca ttaccaatct atccaaggga
    11941 aaaaaatact aatcattgga actggccata ttggaaaaat gattgcaaaa aaatgcaaag
    12001 cattagacat gaactgctat ggaatcaata catccggtca tccagcagaa ggaatggaaa
    12061 agactttttc tttaaatcaa ctaaaggaag ttcttcctga agtagatttc gtagtcaata
    12121 tcttacctct aacggaggaa acaactgggc tctttgatca gcatatgttt gaacactttg
    12181 atccaaaatc tgtattcatc aatgttggcc gtggtgcttc tgttaagaca caagatctgg
    12241 tgcaggcttt aaataaccag caactttcct ttgcagcatt agacgttttt gaggaagagc
    12301 ctcttccaca agatcatcct ctttggaaaa tggacaatgt actgattact tcccacattg
    12361 caggtttgac acctgatttt caaaagaaat taatggctat ttttttaacc aatctgaaaa
    12421 gctactttgc cacccatgag cttcaaacca atcaagtaaa attgtctgca ggatattaat
    12481 agctgaaaac aatattttca aaagaaaaaa gagaaggtga caaaaattgt gtcaccctct
    12541 ctttttttat tgttttttaa agtgataggt tggctcctcc aggttgggat cataatcaac
    12601 aagtaaatca tatcctttaa agaaccattc atctgcttcc tcgataaaaa aaagtgtccc
    12661 atccatttcc actttcttta caggatgttc tggctgttca actgacattc caatcgaaaa
    12721 accatcatga acctctgttt tgccgtagac ttttccaaaa aaacgtattc cgtaagtttc
    12781 tggcagatca atttctgagt caaaccactt ttgtgctttt ggcgttaccg ttaatttcat
    12841 cccaacaacc tactttctct tttctttcta ttgattcatc ttatcgtcta gaatatccac
    12901 atcgcctgtc tctccaataa ccagtagatt atcattttca cgaacatatt catctgctgc
    12961 aggagaagcg ataaccgttt gatctactct tcgtatggcg actactgtta agtcaaaacg
    13021 ttgcctgaaa tttaattcag ctaatgtttt gttgaaaaat ttaggattgg agactcggat
    13081 ttctgctaaa gaatatttat ctgacaactc taggtaatct aaaatattct ttgacactaa
    13141 attatgcgca atccttattc ccatgtctcg ctcgggatgg acgacgcgat cagctccgat
    13201 cttttcaagt actctggcat gatactcatt ttgggctttt gctaaaatat ttggtacgcc
    13261 catttccttt accatcagag taactaagat actggcttga atatcttctc caatagcgac
    13321 aattacatga tcgaaatttc ttatgcctaa tgatcgaagt gtcatttcat cctgtgcatt
    13381 tgccacaaca gcatgagtgg ctatattcat atattcattg acccgatctt cattactatc
    13441 aatagccagt acttcttgac cagcttcgat cagtgttcgg cagatacttc cgccaaatcg
    13501 tccaagacct acaattgcaa aattttgttt caccgttttc ctcctttagt atcttctttt
    13561 ctcagtataa caaagccttt acaaaaacaa aagcaagcat cattattgta aaaaagccaa
    13621 gaaaaataat agttattttc tcagcttttg attatcctct ttatcatcgt ttttatttca
    13681 ccaaaccatg tttgaatgca tagatagcgg cttgtgtacg gtcttccact tctagcttag
    13741 ccaatatatt ggaaacatgt gtttttactg ttttcagtgt aataaacaat tcgtccgcta
    13801 tttcctgatt actttttcct tcagaaatca gcattaaaat ttcattttcc cgattcgtta
    13861 gttcttcatg taatacaggc tcatttcgcc gactcatttt ttccatcatt tttgttgtca
    13921 cttctggttc taatactcgc tcgccacgtt gcgttgccct tatagcatca gcaatttcat
    13981 gtgcggtaga ggtcttcaac aaataccctg ctgcacctgc ttcaatggca ggataaactt
    14041 tttcgtcatc aataaaactg gttacgataa tgattttagc ttctggccaa tcttttaaga
    14101 ttgcttttgt tgattcgata ccatccattt cttccatgac taagtccatt aaaatgacgt
    14161 ccggtcgaag tgtcatcgcc ttctcatagc cttgtcgtcc attttcagct tctccaatta
    14221 cttcaatgtc ttcttgaata gataaataag aagagacgcc taagcgcacc atttcatggt
    14281 catctactaa taaaactttt atcatcctta tctatgcctc cttcatcaaa gggaccttga
    14341 tttctacact tgtaccttga cctttaaaac taatgatttt tactgttcca ccaataccag
    14401 ctactcgttc tttgatattg ttcaatccat aacttcccgt tttcgtttca ttcatattaa
    14461 accctgttcc gtcgtcaata atacgtaaca aaagattatt gtctattttg tgcaaatata
    14521 cctctaattc attggcttta gcatgtctta atgtatttga taacaattct tgaacgattc
    14581 ggaacaagtg atcctcaatg gaactagata gttttacatc ttctacatcc catttcagcg
    14641 aaatctggat tttattttgc aattccttca ataactgctc gataccttgc tttaaacttt
    14701 tctcttctaa attgactgga cgtagatgaa gaagcagcgc acgcatctca gactgggaag
    14761 cgttgatgat ctctgctacc attttcaact gcttgcgaaa catttctggt gtctcgcttt
    14821 tttctgcttg ctccgtcaat gccgacatca tcatcattgc tgcaaacaat tgttgcgaaa
    14881 ctgaatcatg cagctcacgg gctaatcgat gtctttctaa ttctaaaatt tcttctttgg
    14941 tttgtccatc tacatattgg ggacggctcg ttacaatttg taattcacta gatacttcaa
    15001 tcattttttc ttttatctta gtgatttcct tatcaatatc tttgatgtat aaatcgtccg
    15061 atgcacttgg gatcggaaga agcaacaatt tggattcata attgccagaa gaaagtgcac
    15121 gcagcttttc ttcgattttc ccgtattttg tcttttgaat aagtgataat aatagaaaga
    15181 caattaatcc tacacctaag gaaatggcta acaaatggaa gataagcgga acatataata
    15241 aacgtgcacg cagtaattct ccccaccaat gactttgatt gctggcataa aaataagtga
    15301 aaagtgaaaa tagaataata aggaaagccg caatccccga ataaacagct agcatcgctc
    15361 tggatatttt tcccatcata ttcgtatcac ctcgacatct ccaatcaagg tattagtaat
    15421 aatcttcaat ctacgagggt tttcatcata atcactgctg tagattttga tttgttcatt
    15481 tttcaaagaa aattgctctt cttcaaacaa gacatttcct actaatgtcg catgctctaa
    15541 tcggatggct acgcccaaag gtactaagat ccgtgttctg cctatccctt ttcttacaat
    15601 cacaatatta tcatcttttg gcaaaagcgt attgcccaaa tctatgatag tatcaccaga
    15661 aatgacggcg atgttgatat cgtcccattc atagacgtcg tttccaatac gttggttacc
    15721 gaataattgc tgtcttttcc gctcattatt atgtgttttt aattgctctg tttggatcat
    15781 catgatctgt ttctttcgcc aaaaagcatt tttggttaaa tctatccctg aaatctctac
    15841 acctttcaga ccaataaata aaacggcaaa tacagccatc atccataaag caggactgtt
    15901 gactaagcta aaaaaaatga ccaaacttcc taatatcaat tgaaaatttt gaaatttagt
    15961 acgaggatgt ttgcgcatag ctagatatat attgaaaata ccgaatatca ctaaaagcaa
    16021 aagtccagta ttgtttacga tttgccaaac agcaaataac agcagtagtg cttcaactac
    16081 cacaaaaaaa cgccaagaac tattcatcgc tctttctccc tcctgacaca ttctcatttc
    16141 atcataccta tttttttaac tgttggctat aggactttag atgtaagatc gtttgttgaa
    16201 atctaaaata cacaaagaga aaaagatcta gagtgtatca tcagtaagat gaactctcta
    16261 gatctttcag tcagactacg cttgttctac tttgacgatt ttaactaaca tatcgccacc
    16321 tggagtagca atagttactt catcattttt acgtttgcca atcaatgctt gcgcaatagg
    16381 agaatcattg gaaattttgc cagaaaacgg atctgcttca gcgcttccga caatcgtgta
    16441 ctcttcttct tctccatctg gtagttcgat gaatgtaact gttttaccaa ttgatacctc
    16501 atctgcatct acaccgtcgt tatcgatgat ttgagcaaaa cgaatcatat tttcaattgt
    16561 tgtgattctt ccttcaacaa atgcttgttc atctttcgct gattcatatt cagagttttc
    16621 ggaaagatct ccgaaaccgc gggcaatttt gattcgttca atgatttctc cgcgtttgac
    16681 tgtttttagt tcttctaatt ctttttctaa tttttcttta ccttctagag tcattggata
    16741 aactttctct accatgtgta acgtctcctt ttttcgataa aataccgcaa aaggtaatct
    16801 tttgaggaat atagtccaaa aaagattacc acgcttttct tcaaatgtaa atgtttttct
    16861 aaaaatgatc aatgtctaga gcctgttttt ctaagaatta tttacatatt tttccactaa
    16921 ttccatatgt tcttcataag ttttagcgaa ataaacattt ccagtgctcg tatctgctac
    16981 gaaataataa tagtcatttt ctgctggttc taatactgcc ttgattgcct cttcgcttgg
    17041 attgtcaaaa ggtccaggtc catagccggt attggtatac aaattatatg gtgaatctac
    17101 agctgtatct tcatacgtca ctaattcttt gtgttcacct aatgcgtata aaacagagat
    17161 atctgactga agtggcatat ttgctttgat tcggttaaag aagacttgtg caatgttttt
    17221 acgatcattt tcttttacgc cttctttttc aactagagaa gctagagtta aaacttcttg
    17281 tacagtcaaa tttttctgct tgatagctga ataatagttt tgcatgaccg tattcgtctt
    17341 gttcaccatt tcaatcacta gatccttaag cgaagtactc gtgtaataat catatgtggc
    17401 tgggaataag taaccttcca aacggtattt tgtgttctca gctttagatg cactttccaa
    17461 cagttctggg aatttttggt gcaactcgtt aaagaaagtc tcatcattca ctaaatctaa
    17521 aaattctttc ttgtctttac cagttacctt cgctacccgt tcagcaatct gatcgatatc
    17581 gtacccttct ggaatggcaa ttttcccatc tgcaactttt gttggctcag aagtaccgcc
    17641 ttcttgcaac tgtttgccaa tctcgtcaag agtcatatta ggcgctaact gataatagcc
    17701 agcttgaaag ccagttaaat ttttaaattt tgtataataa ttgaaaacaa tcccgctttt
    17761 aataatattg tccttctcca aaatctcacc aatctgcttg ttagaagagc cactcgggat
    17821 ctcgacttgg actaattggt catctgattt atctaaaggc ttcaacccgc tatctacata
    17881 acgatacacg gtaaatccaa gaaatccgcc aataatcaat aatgtaagag caatcactaa
    17941 aatgatcttc ctcacaatct tatcttcttt tttacgcaga tttctccgct tgtcgattat
    18001 atctgctgta gctgccttac tggcttcttt tttatcttta cttgggtgtt gtttgtttcc
    18061 tgacaaaaca actcctcctt actatcaagt tcgtaacttt attcattata catgatattt
    18121 gaattggcgt gaatgatttt atttaaatat tctatcaaaa atgtaagtag taaatccatt
    18181 actataagat taaaataaat gttatcaaga tacaagctat tttagcttta ttctttctgt
    18241 aaaaaagaaa ggagactaaa taaactatta tgaaaaaagg aaaacatgct aaaaaaagaa
    18301 aatattcatt tttaatgatc gttgtcgtgt tgtttagcct tagtgcgttc tctacaggaa
    18361 ctattcttgg atggcaattt tacaataatg aacactctca gatagttgcc catgctcaat
    18421 cagctcaaaa aaagttgata ctgactacca atgaacaaca gctcgttgaa aaaatcgatc
    18481 aactattaag aaaaaatgat tatattggct caatttacgt aaagaaaaat aatcgcatca
    18541 tcatggaaaa aggctatggc tatgcgaata aactcacgca acaaccaaat gatccttctc
    18601 tttattatca gattggttcg gttcaaaaag cattgactac tcttttgatt ttaaaacaag
    18661 tcgagcgtgg gcacctttct ttagatacta agctggctca attctatcca caaattgctg
    18721 gcagccagca aattactatc cgtgacttgc tgtatatgcg ctcaggcttg caccgaactg
    18781 cttctccaac cataccgatg actgatgaag aagtcgttca atttgcgatt cagcacttaa
    18841 aatttacaga ttatcagacc tctcattatg attcattgaa ctttaccata ttgactggaa
    18901 ttttgatcaa attaacaaat caaccgtatg aaaaattatt gaccgatgat atcatcgaac
    18961 ctttaggatt acagcataca aaattttatg accaagtcaa aaattcatcc aatcatgcac
    19021 tatcctatca aatgtctaaa gaggatgatt actttaaacc actgacagaa tctgaaacag
    19081 agatccataa cgaattaggg actggtaata tcagtatgtc cgtctatgat ttaaatacgc
    19141 tgtttacaca agttttatct ggaaaactag tttccaaaga cttactcttc tcattatggc
    19201 gtgaaaatgc gaatggacat ccatatagtg gtggggtata tagcggagat aattatatcc
    19261 tgtcgcaagg aaatatcgac tcttttgata catctgctat atttagcaaa gattcacagg
    19321 atgctatcat catggaaagc aatataaaag ctgacaaaac tatcaaactg cctactgctg
    19381 atctaagaaa ccaaatctat gacctgatgg aaggaactac taagttggca aatagctgat
    19441 atgatgaaaa gatagcaaat actacttctt tgaatagcat ttgcttaaaa aaagtactcg
    19501 actaaacgta ggaaagagtg tgggacaaat ctaaaaatca atttttgcta cagactttaa
    19561 ttccaaataa acagaacaga aagaggtgtt cttctttctg ttctgtccaa attattctaa
    19621 gatttctctt taccaacatt atttgacaaa gggcctatta aatctaaaat agtatcttta
    19681 gattgctaaa atatgtgcta gcaaatcatt catggcttgt tcttattttt aattttatgc
    19741 aataaaaaag aagaacaatt agcgtgatta tagaaataaa taatgagatt ttagaaaaaa
    19801 atgtgtatga atacctgatt gttactttat ttattccact ttcacttttt actgtaacaa
    19861 aaggacctcc ttgttttagt actgtagcat tttttgaatt aacagtaacc gttaatcctt
    19921 tataccggaa tacaggtaaa actattgttt tgccttttga agatttaaac gtaaatgtaa
    19981 tttcatcttt atttggagaa aagctactat aaataacttg accatcttct gtaacttgat
    20041 ggtttttcaa aagcaattga tcttttttat agcctatagg tacataatca aaattctcaa
    20101 attcttcaag tttattttcc atttgttcat tagaataagt tccttgaaaa ttcatttctt
    20161 tcaccatgtt aactactgat gaataattta aaattgaaat agctaatatt aataatagta
    20221 taaaatttct tctccttgta taagaaaatt tttccaactg tggagaaata aatagagata
    20281 ctgaaaaaga tatgaacaat gttgaaaata tcataaaacg ccaaggagct tgaataatat
    20341 ttaaaggagt attttgtaag gcaaaccatg gaaatatttt tgtacaaagt agaatagata
    20401 ttatacccat tatataagtg attttagata agattcctaa ttttttaaag ttaaatgtta
    20461 gtataattaa acaaataagc aaagttaagc caataccaaa tgatagtaat tctgaatcta
    20521 tacttccaat caaaaactct ttacccagat aaattgctct tttttgtaat atatccacat
    20581 aaggtctatt cagatcttga tacaatgtct gctcaatcat aggacctata aacgctagtg
    20641 aaagaagtaa agtaactata gcagctttta ttaatttaat aattaagaag atttctattt
    20701 tctttttccg ataaaattga ataattaaag caattataat cattaccata gtcatagcta
    20761 cagaaagaac atgagtgtaa attaataaag tcatgctaat cgccaacata acccaagatt
    20821 ctttttttga gtcgtaaagc tttataaacc cgagtaaaat aagaggaagt attgacattg
    20881 cgagtaattc ccccattgcc cctcgaagaa atatttctac actcctaaaa gaagatgttg
    20941 tatatataat agcgaaaaag gagctagtaa catgtttttt ggtaatttca tacattacat
    21001 aatgacatat aaatagagta atgagcgtaa caatataaag aaaaaggtta tagcctattg
    21061 ctaaattagt aaacttgatc aataaaaata aaggatatag agttaaccat gggtaaaaat
    21121 tatttacttg catgccatat gatctaaacg tttcaaaatt aataggggag ctaaaaatag
    21181 attctaaact taacgcacga ttaatgtgaa atgccgtatc ataaaagcca aatatttcca
    21241 tgtttttaaa tgctggaaat aaatataata aactaacaaa tgcataaact ataaatattt
    21301 ttacagaaaa aatttttttc aaattcatat accacctcat gtttttttat attacatata
    21361 gtatcattta tttttttaaa acactagatt actcactata taaaatgtga ttaattttat
    21421 tattaataaa atatttttta tattacaaaa gtagtgtttc aaagttagta gagaaaatgt
    21481 agttatatat ttaaattttt accagaaaga aatgaacacc ttcaattatc taaatctcga
    21541 aaatggttag atgtaatcat caggaaaaac aatcttaaac gaattactat tcatggtctt
    21601 agacacacac atgctagctt gcttttagca agctagtgca aatattaaag atgtgcaaga
    21661 gcgtttaggc cactcttcta ttcaaatcat tatggatcta tatattcata ttacagacaa
    21721 aagaaaagaa gaaaccgtag cgcaattcgc aaaatatagg ctctataatt tatgggactg
    21781 atgaatcaaa attgattctt cagtcccatt gttattttag gtatatgatt cgtacattga
    21841 aagagttgaa aaactatctt ccgaagccgt caaaaaggac cctgccaaaa gtattgatgg
    21901 tggatgaatt tcgttcgcac gcttcaattg tggataaaat gagctttatt tgtgcggatg
    21961 gcgagactgg aaaactcatt gatattttgc ctacacgaaa attgtctcga ttaacacact
    22021 attttagtcg ctgtgcaaat cctgaagcgg tggaatattt agtgacagat atgaacgccg
    22081 cctacttcca actgacaaaa cgtgtcttac caaatgcgaa agtcgtcatt gattgttttc
    22141 atatcatcaa acatttgaat caggcgttca atgattttcg tgtacaagaa atgaaaagat
    22201 tacaccagtt agggcaacga cgggaagccg agaaagtgaa aaagaactgg cgctttcttc
    22261 taaaaaaccg tcaaaaaatc aatcactatg attacaaaac atggcgcagt tttcgtgcac
    22321 ctaaataccc gctactcact gaagcaatga tgattgatcg attgctggca ttttcaacgc
    22381 ctttaaagga cgcctatact tttttccatg agctgaccga agcctttaga gacaaggaat
    22441 cagacttatt ttttacct
//
LOCUS       DACDXO010000044        22268 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E23605_2017
            SAMN12639989-rid9881463.denovo.044, whole genome shotgun sequence.
ACCESSION   DACDXO010000044 DACDXO010000000
VERSION     DACDXO010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639989
            Sequence Read Archive: SRR10023389
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741914.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:42:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,972
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 33 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 17 of 73
            Pseudo Genes (multiple problems)  :: 10 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22268
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639989-rid9881463.denovo.044"
                     /isolate="E23605_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            2..400
                     /locus_tag="IWM35_001851"
     CDS             2..400
                     /locus_tag="IWM35_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HAP9919699.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
     gene            384..1079
                     /locus_tag="IWM35_001852"
     CDS             384..1079
                     /locus_tag="IWM35_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HAP9919700.1"
                     /translation="MEIMLDTANTKSIYSCQQFLNLTGVTTNPTILKKEGASNFFSHM
                     KEIKKIIGQIPIHIQTIGRTEEEMIEDARVIIEELGKDTFIKVPVNQAGLQAIKKLKK
                     EGYLITGTAIYTELQGYLAINNGADYIAPYYNRMQNQGIDAMKVIDSLRTEIERTQRP
                     TKILAASFKNCEQVTTAIRSGAHAITLGKELLDQVLEMPSIDKAVRGFQEDWVQTFQA
                     ETIGELKKVADYF"
     gene            complement(1673..2560)
                     /locus_tag="IWM35_001853"
     CDS             complement(1673..2560)
                     /locus_tag="IWM35_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rotamase"
                     /protein_id="HAP9919701.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEEAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRK
                     VMKKDNVTIKDPYVKNIFK"
     gene            2745..3584
                     /locus_tag="IWM35_001854"
     CDS             2745..3584
                     /locus_tag="IWM35_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAP9919702.1"
                     /translation="MIRIIRLIGSVLIVLVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNWTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            3780..4700
                     /gene="sgrA"
                     /locus_tag="IWM35_001855"
     CDS             3780..4700
                     /gene="sgrA"
                     /locus_tag="IWM35_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HAP9919703.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSESSTENTSSESSTESTSSESSTGSMSSESSTGSTSSESSTGKVN
                     NELDLVPIIPTKEQKRNAGSNLKSVLKQPDFVSRGIDHQEKAKETDNRKSDLPETGSR
                     TLNKLITWMGVLLILIVGASYFRSLFHRVK"
     gene            4998..5354
                     /locus_tag="IWM35_001856"
     CDS             4998..5354
                     /locus_tag="IWM35_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9919704.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEFSSQFLLSKVYSILLEGTIGYILLDSL
                     YKGTYQSLENLSQKII"
     gene            5357..6514
                     /locus_tag="IWM35_001857"
     CDS             5357..6514
                     /locus_tag="IWM35_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9919705.1"
                     /translation="MRTIQRKLRELNTILKNYHLSCSLKKGNPIEGEEYRIRYFFHLM
                     YWQSFDENHSDILSSIKNKRQLLEKFAKHAPYTRSIDRKKWISLLRISLQRMKQGHSI
                     TYVPEEMNKFVHPLITKEEFTHYILEPFFEANFWSMNHITPTEQAYLYFMFGVMNTYL
                     EEDLLDTPDKVQESWGWIESSIVENFEKNFKICFTTSEKNYLYVNLAMIHLYSRVFGT
                     KKKVDAFGKSAEDRDFQHIFPVMYPIMQQFFSGVARRIPELNTYYEKNRRLIFQYCML
                     VRDIFIKHEQPVIFYIQSKYGKTQELWAKKRILSMTERPIKYSASADQLPDIVISDYP
                     IDKKAYDQTNTQIFYWNGQPTKNDWARLKAEIIKIRNEKNRSILEKLDFVN"
     gene            complement(6540..6980)
                     /locus_tag="IWM35_001858"
     CDS             complement(6540..6980)
                     /locus_tag="IWM35_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAP9919706.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            7140..7646
                     /locus_tag="IWM35_001859"
     CDS             7140..7646
                     /locus_tag="IWM35_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP9919707.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            7814..9726
                     /locus_tag="IWM35_001860"
                     /pseudo
     CDS             7814..9726
                     /locus_tag="IWM35_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
     gene            9731..10732
                     /locus_tag="IWM35_001861"
     CDS             9731..10732
                     /locus_tag="IWM35_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP9919708.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(10934..11140)
                     /locus_tag="IWM35_001862"
     CDS             complement(10934..11140)
                     /locus_tag="IWM35_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HAP9919709.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
     gene            complement(11786..12634)
                     /locus_tag="IWM35_001863"
     CDS             complement(11786..12634)
                     /locus_tag="IWM35_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP9919710.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
     gene            12798..13700
                     /gene="gnd"
                     /locus_tag="IWM35_001864"
     CDS             12798..13700
                     /gene="gnd"
                     /locus_tag="IWM35_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HAP9919711.1"
                     /translation="MEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDI
                     PAYQEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSI
                     RRAEEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLY
                     AGASGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWL
                     MEIMEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEED
                     SFSAKVVSALRNGFGGHEIVEKGE"
     gene            13702..14703
                     /locus_tag="IWM35_001865"
     CDS             13702..14703
                     /locus_tag="IWM35_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HAP9919712.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALVERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            14700..14984
                     /locus_tag="IWM35_001866"
     CDS             14700..14984
                     /locus_tag="IWM35_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAP9919713.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            15017..15814
                     /locus_tag="IWM35_001867"
     CDS             15017..15814
                     /locus_tag="IWM35_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAP9919714.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNAAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            15828..16661
                     /locus_tag="IWM35_001868"
     CDS             15828..16661
                     /locus_tag="IWM35_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP9919715.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGVFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            16679..17536
                     /locus_tag="IWM35_001869"
     CDS             16679..17536
                     /locus_tag="IWM35_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP9919716.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLMQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            17558..18067
                     /locus_tag="IWM35_001870"
     CDS             17558..18067
                     /locus_tag="IWM35_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP9919717.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            18104..18505
                     /locus_tag="IWM35_001871"
     CDS             18104..18505
                     /locus_tag="IWM35_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9919718.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            18617..18970
                     /locus_tag="IWM35_001872"
     CDS             18617..18970
                     /locus_tag="IWM35_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9919719.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            19004..19447
                     /locus_tag="IWM35_001873"
     CDS             19004..19447
                     /locus_tag="IWM35_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAP9919720.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            19464..20549
                     /gene="uxuA"
                     /locus_tag="IWM35_001874"
     CDS             19464..20549
                     /gene="uxuA"
                     /locus_tag="IWM35_001874"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAP9919721.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            20542..21255
                     /locus_tag="IWM35_001875"
     CDS             20542..21255
                     /locus_tag="IWM35_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP9919722.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
     gene            22007..22207
                     /locus_tag="IWM35_001876"
     CDS             22007..22207
                     /locus_tag="IWM35_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP9919723.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
BASE COUNT         7665 a         3421 c         4605 g         6577 t
ORIGIN      
        1 gatgacgatc tttgaaacgg ttgtaacaga tgttggtata gaagcagaag tattcaggaa
       61 agaaaaaata atgattttat ttggagatga agcaccggaa acattaacag attattgtta
      121 taagattgaa ttgaatcagg taagaaaaga aataacttgt gatatgaagc tctgttttga
      181 taaacaaatg tatgtaatca cagcagtagg agatgttgtc caaactaatt tgaatgaatt
      241 aggacatatc acgataaaat ttgatggatc tcatgagccg gaacttccag gaacaatgta
      301 tgtagaagat aaggaaatgc cgcatttaag aaaggggaca atcattaaaa tcgatcaaga
      361 ggatcagaat gggggaaagc tatatggaaa tcatgcttga tacagcaaat acaaaaagta
      421 tttattcttg tcaacagttt ttgaatttaa caggcgtaac aacaaatcct acaattctga
      481 aaaaagaagg agcgtcaaac ttcttctcgc atatgaaaga aatcaaaaaa ataatcggac
      541 agattcctat ccatattcag acaataggaa gaacggaaga agaaatgata gaggatgctc
      601 gagttatcat agaagaactg ggaaaagata cgtttatcaa ggtgccggtg aatcaagcag
      661 gccttcaagc gatcaaaaag ttgaaaaaag aaggttattt gattaccggt acagctattt
      721 acacagaatt acaagggtat ttagctatta ataatggagc agattatatt gcaccttatt
      781 ataacaggat gcaaaatcaa ggcattgatg ctatgaaagt gattgattcg ttgcggactg
      841 agatcgaacg tacgcaaaga ccaacaaaaa tattagcagc tagtttcaaa aattgtgaac
      901 aagtcactac agccattcga tctggagctc atgcgataac gttaggtaaa gagctactag
      961 atcaagtatt agagatgccc tcgattgata aagcagtgag gggcttccaa gaagattggg
     1021 tgcaaacttt ccaagcagaa acgattggtg aactaaaaaa ggtcgctgat tacttctgaa
     1081 tcaatttctc cataaaataa acgatagcct tctcgaaaac tcgaggaggc tatctgacga
     1141 ttcaatattc attaatttaa agtttgggtg actattacta actattactt atttctcatt
     1201 taaacgatgt attccctaac ctctaataca tccttgtaac tctgaagtta cgccagtttt
     1261 gaataaattt cataaaaaca aatgaaaagt gtcgcataaa aatatctttc gtgtttctct
     1321 agtataacag aataagaaga acaagtccag aagggatatt tttagtcttt tttaaaataa
     1381 cttttcagtc gagtttgagt ttatgatagt tgtttttgaa gcgatacggc aagtgaaaaa
     1441 atagggaagc atgaaagaat ctacttaatg gcgcattgat tcatgtgata gaaataaaaa
     1501 acttttttaa attatatcct catgattgta acaaatccag tgatttgttt tggtacaaat
     1561 aaaaaaaggc cccgatgaag ttgtggcaaa agtggtttgc ctcaaaaata agaaggaatt
     1621 tccaaaaatc acttctcaaa ttttgccaca tccccaattg ggtcaaattt gtttatttga
     1681 atatattttt tacataagga tctttaatag ttacattgtc ttttttcatc actttacgga
     1741 ttgttttatc catgaattcc gtatcaagag tttttgcttc ggtagcaatt ttttctagct
     1801 cagatttgta tttgtcttta ttagaacctt tatcctgttt ttttaccatt tttactagat
     1861 aatagctttg ctgataagtc gttggatctg ttacaggaat gatatcagaa acttccccgt
     1921 tttttagttt aaatgctgct ttttttactt cagtaggaat ctctggattg gttgaatcaa
     1981 aattcatttt tccatcagtt tcctttaatg ttttattttt tccataagct tgaacaagct
     2041 tggagaaatt ttctccttca tttgcttctt tttttgcttt atctgcttct tctttagtcg
     2101 aaaatagaat gatctgtgta ctaacttctg gatagaattc tttccaagca gtgtctagat
     2161 ctttatctgt taattttaca ttggctttca aaccgtattg gaaagccagc atctgtttaa
     2221 agagtttctt atatgtctct tctgtttggc caactgcttt taattgttcc ttgtagtttg
     2281 gaccgagttt tttgatttgt tcattatatt cttttgttac ttcctttgaa gaaatctctt
     2341 ttccgtatac atcatcaaat gctttgaaga caatcatttt ttttaatagt gtttgattat
     2401 ttgatttaac ttttgggtta tcataaaaat cctgaactgt aagtgttcca cctttaaacg
     2461 ttgcaatcgt ttcattggac ttgctacaac ctgagaatag taaaattgca catgctgaaa
     2521 ggcacataat tagtagttgt tttttcaaaa aatttcccat cctttcctta ttaaaaacag
     2581 tctaacagaa gaattaggga gacagtcttt cattttcgta aggttgctgt agatattctt
     2641 agttcgtaaa aatctattct ctctatgatg gagaacattt acatagtttt cacgagacaa
     2701 gaaagaaaag ggttgttaaa cttaatataa aggagggatg aaagatgatc aggattattc
     2761 ggttgattgg atctgtttta atagtcttag ttggttttgt tttaggaatt cttgtcaata
     2821 atgcacttgc ggatatgttt attggaaata gtgaatggat tggagcaatt gccggtacgg
     2881 tcggacattt agctgttttt ttactagctt tatttcttgc tagtaaggtc gaaggaaaaa
     2941 gaatagaaga ttatggtgtt tctgttcgag gaaaagactg ggggtattta gcgggaggtc
     3001 tcgttgttgg aattggagtg tttctgctta ttactagtcc attatatttg acgggggcgt
     3061 atcggttaga ttcaggaaac gcaaacattg tgccgctgat aacgagtttt attttattta
     3121 ttgcagtggg ggcatcagaa gagcttttat tccgtggctt ttttcagcat cagatgctta
     3181 gatttggtcc gttaatagca atgattggat ctgctgcatt atttgctctt ttgcacggat
     3241 tgaatcctaa catgacattt ctggcagttt tcaatatctt tttagcaggt tgctttttca
     3301 gcgcacttat ctacagtaca ggaagtttat ttacggctat tggtgctcat attacttgga
     3361 attggacaca aggagctatt ttgggtatac cagtcagcgg aacgcaagaa caaggttttt
     3421 tctccactct gatccaatcg caaaatacaa taattactgg gggaaacttt ggggctgaag
     3481 catctatatc aactacatta ttcttgttag tcttgattgt tggtttgtta ctctatgcat
     3541 ataaaacaaa gcggatgaag aaatacactg ctgtttatca ataaaagatg aagaaagcaa
     3601 ggctgatatc ggtcttgctt ttttaagtct gtcattgatt aaacgaaaaa gtaaacaaat
     3661 tcaagcgaat acacgacatc ttttgttaga ttttcacaac tgactgtcgt gttctcatca
     3721 ttttcatttt atataattcc tttatcaaat agaaggtaat tatatggaga gtgatagaaa
     3781 tgaagaaaac agcgaccgta ttatttgtta ttcttgggct gagtataatt gcagcaatga
     3841 ttggaatcag tacttctgca aatgaacggg caaatgagaa tctttcattt actgtgaaaa
     3901 ctgatcggat tgtttatgac atgacacaac aagtcattac tattccagtc aaaccaaaca
     3961 aaagcgtgaa tgctagtgat gtacatgctg ttctaacata tggttgggat ggtaatggaa
     4021 gttcagaaaa agttattggg gaagtttatt taaaagacgt acaatggacc gctggtattg
     4081 agtatacaat aatgatttct gcagaactat cgattgatga gatcaagagt aaagataaag
     4141 ttgatttgat tgttttttat gatggacaga tgacgatcac ggagaatttg aaaccaagca
     4201 gttggacagt tgttggaccg ccaagcagta cggatagcac aagtagtgaa agcagtacag
     4261 aaaacacgag cggtgaaagc agtacagaga gtacgagcag tgaaagtagc acagaaaaca
     4321 cgagcagcga aagcagtacg gagagtacga gtagtgaaag tagcacagga agcatgagta
     4381 gtgagagtag cacaggaagc acgagcagcg aaagcagcac aggaaaagta aataatgaac
     4441 ttgatttggt acctatcata ccaactaagg aacaaaagag aaatgcaggg agcaacctaa
     4501 aaagtgtgct aaaacagcct gattttgttt ctcgtggaat tgatcatcaa gaaaaagcga
     4561 aagaaacaga taatagaaaa agcgatttac cagaaactgg tagtagaacc ttgaacaagc
     4621 taattacatg gatgggtgtg ttactcattt tgattgtagg agcaagctat tttcgttctc
     4681 tcttccatcg agtgaaataa agaataaaag caacagatct aaaattgaat aaaacgatag
     4741 atttttgagt gaatatttca ttcgagagtg ctgtattgcc tggcgaagtt gtagctttgc
     4801 cagatctatt tttgtttact aacatatttg agaaacagga aataacagga acacaaatgg
     4861 aaaataagag caagaataat tgacaatgca cactgcgcat ggtagtatct ctgaaaaatc
     4921 agtaacgaat ctatttgtac gttattcatg tagctgtcgg aataagtcta aataatagat
     4981 aaaaaggagt atgtactttg tttgaagaat ttattgatat caatgaaagg caagtatatc
     5041 aatttttgaa ctattgttat gaaagagacg agaaattata tgtggtgaag gatatcgctt
     5101 tagatctaaa ctacaccctg gctaagatga attcggtgat tcaacaagca gaatcgtttt
     5161 gcgaaaggta tccagaatat aaattatctt ttctttcaga aaacaagatg ataaaagtcg
     5221 agttttcttc tcagttttta ctgagtaaag tttattctat acttttagag ggaacgattg
     5281 gttatatttt actggattca ctttacaaag gtacttatca atccttagaa aatcttagtc
     5341 aaaaaattat ataagtttgc gtacgattca aagaaaatta agagaactta atactatttt
     5401 gaaaaactat catttatctt gcagcttgaa aaaaggtaat ccgatagaag gagaagaata
     5461 ccgtatccga tacttttttc acttgatgta ttggcagtca ttcgacgaaa atcattctga
     5521 tatattatca agtataaaaa ataaacgtca actattagaa aaatttgcga aacatgcgcc
     5581 atatacgcgt agtatagata gaaagaaatg gatcagccta ctaagaattt cgttgcaacg
     5641 aatgaaacaa ggccactcga ttacgtatgt cccagaagag atgaacaaat ttgtgcatcc
     5701 attgataaca aaagaagagt ttacgcatta catactagag cctttttttg aagcaaactt
     5761 ctggtcgatg aatcacatca ctccaacaga gcaagcttac ctttatttta tgtttggtgt
     5821 aatgaacacc tatttggaag aagatttgtt ggatactcca gataaagtac aagaaagttg
     5881 gggctggatt gaatcgtcta ttgtagaaaa ttttgaaaaa aacttcaaga tttgttttac
     5941 aacttcagaa aaaaattatc tatatgtcaa tttggcaatg atccatttgt attcaagggt
     6001 ttttgggaca aaaaagaagg tggatgcttt tggaaaatca gcagaagaca gagattttca
     6061 gcatatattt ccagtgatgt atccaatcat gcaacagttt tttagtgggg tagctcgcag
     6121 aattccagag cttaatactt attatgaaaa gaacagacgg ttgatttttc aatactgtat
     6181 gttagtcagg gatattttta taaaacacga acaacctgtc atattctata ttcaatcaaa
     6241 gtatggtaaa acacaggaat tatgggcaaa gaaaaggata ttatctatga ctgaacgccc
     6301 aataaaatat agtgcatccg ctgatcagtt acctgatatt gtcattagcg attatcctat
     6361 cgacaaaaag gcatatgatc aaacgaatac acagatattt tactggaatg gacaacctac
     6421 aaaaaatgat tgggcgcgtc tgaaagctga aatcataaaa atcaggaatg agaaaaatag
     6481 atccatattg gaaaaattgg atttcgttaa ttaagaaaag cagattgttt atgccaaaat
     6541 taagacattg caaaaagatt attttcttta aaatcgacaa gatgaccaga aagcaagagt
     6601 tcgttgagct cttgctttct tttagtttta gaaaggaaca tcctgatttt atcttcgcta
     6661 aatccagtca aaagttttgc agattggtcg tagataatcg gttttttcaa taaagaaggg
     6721 gatgtacgaa tctcaatata tagttcactc aaactgagtt catctattct gtgaacaaga
     6781 tttttataac taatagaatg agtagagagc atttcggtca cgccgttgtt tgtcaatttt
     6841 agtaagtgga taaattcatt tttggttagt ggttgacgaa ttaagttctg ttcgataaac
     6901 ggtatatcat ggacgataag ccaacgtttt gcgcgtcttg aagacgagca gccagaacct
     6961 gtatacaatt taatcatcat acattcctcc aatagctaag tatttatgaa ataataacat
     7021 gaagaaatag tcgaaaagtg ccatttagag gtgtaaaata agaaaatgtt gtcacgtata
     7081 atggaactag ctagataaga aatactatta aatcaagaaa aaagaaagaa ggcacaagta
     7141 tgtcaaacac agccgacgca atagtagtga tcgatttaca aaaaggggta tgttttgatt
     7201 cttcgccgat acacgaatta gaaacagtga cagcattagt gaataaaaga atcaaaacat
     7261 acgaaacgat tgggaaacca gttattttcg ttcaacacga agatgctcaa ttagtgccac
     7321 aaacaaaacc atgggagcta ttgtcggaat tatctattcc tgagcacagt tattttgtga
     7381 gaaaaaccca tgcgaattca ttttataaga ctaatttgag tgttttatta actgaattgg
     7441 actgtcattc gatcgaatta tgtggggcac aagcagagta ttgtatagat acgactgtga
     7501 agtttgctca tggattagga taccagctta tgatgtgtag aggagcaacg acaacttatg
     7561 ataatccttt tatgaaagca aaggaaacaa tcgcttttta cgagaaaatt tgggataaac
     7621 gatttctgac atttttggag caataagttg tttcttcgtt taaaaaaagc agaatggata
     7681 ctggtttatc cattctgctt tattacgtaa aatgagaaaa taatcctgtt ctatttttat
     7741 gggataaacc atttattttt tgtataaaaa atgtttgcgt ttacatataa ttctcctaga
     7801 aacataaaaa ggagtgaata gcttgagaac aaaacaagta ttaaagggag cgttggtcat
     7861 cttatttgct gtaagtacaa gcgtcccgca atggacagta tatggagaga gcatttcttc
     7921 cgaatcggaa tctcgggagg tagaaaccac agaaactact gatgctggaa atgaaacgaa
     7981 atcgagtact tcacaaacag aaacaaatac tgaaacaacg gttgagacat ctgttactag
     8041 cacagatcaa gaaacgaccg ctaccacaga taaccaacaa acagaagaag caacagctac
     8101 aaaaatgtca aacgacgaat cctcttctgc taaagtaagt gacaaagaga atgggacaga
     8161 aaatgatgat atcggtaaag gtgaaaattt caatggtaaa gaatatttga attacttcgg
     8221 taaacagatg acagaaatac ttaatggacc aacagaagac atcaagtcaa aattaataga
     8281 gttattagtc actgagcttg ctgttaatga ttatggtgta gataaagaga ttatcaaaca
     8341 tctgattgct acttatctat tgtacgatat cgcttttgca aaaaacagtg tgttgatgtc
     8401 gatgcttgga ggtacagatg atgggacaac gcctacagaa aacacaggct tctatccaaa
     8461 tacaaaacaa cttcatcaaa ctgtactaaa caaacaagat ggcaaaatga tggatgtata
     8521 tgcatattat gtcgatcaag gttcagataa aacagtcatg atccatggcg gatttcgagg
     8581 gaattggaat aacgggatcg ttacagaaga atacaatgat ttttataagg ccgggtataa
     8641 tttattattt gttgattcca gagcgactgg taatagcgga ggcgattatg tgacctatgg
     8701 acaatatgag tcagatgacg tcctttactg gataaatcaa gaagttcgag agcggccatc
     8761 tcaaaagatt ttactttatg gtggttcgat gggagcagct acgatgatgt ctgtattagc
     8821 gaaagatatt cctgtcaatg tcaaaggaat tattgaaaat tgcggatttg catcaattga
     8881 tgaacaacta cgttttacgt acagccagac agtagccccc gcactaccgg atgccataaa
     8941 aaatcagtta gacatcattg gggatcaaga gcatgaagat ttatttatgg gccttttgaa
     9001 acaatactat tttgatcagg agatgcattt ggatacaaaa gctgctctac ctacgattgg
     9061 aatgtcagat tcattgccta aattgattat ccatggaaca gcagatgatg tagtacctgt
     9121 gtctaacgcg caaaaattat atgagttatc aggagggtat aaagatcttt tattagtaga
     9181 aggagcaggg catggaaagg cacaggaagt tgaccatgct gcctacacga aacatgtaac
     9241 ggactttttg aaagtagtgt tccatgatca gataaacgta aagtatgtgg atgaaaacaa
     9301 taaaagtcta ctcaaagacc aggacgaaat ccagctttac ggagcttatg gagaaaatta
     9361 tatgacagag caaaagacat tcgaaggata cgaattagca aatgttgaag gaccgactga
     9421 agggattttt aatgagacga taccaactat tattttctat tacaaaaaaa tcccagtagc
     9481 tccaccaaaa aagcaagatc cgacagagaa tgcagacaat aagggaaaga atgaacctcg
     9541 agaggatcaa tcaagtgatg atacgcaacc gattcagatg acagtgaaag tacaaaaaaa
     9601 gacagatgca aaaaaagaat gttgccgaaa actggagaaa agaaacattc gcttctcatt
     9661 tattcaggtg gatttttatc tgttagcacg attatcacat ggatttggcg aagaaagaga
     9721 gggtaaatga atgaaatggt tcaagcgaat agtgattgca gtaccagtta tcatcgtagt
     9781 tattttagtc actgtatttt tgattaatca aatcacacca aagccgatta gcttgcttgt
     9841 tagaaaacaa tttgacacaa gtgaaaaaga acaagtatat gcccgaccag atgatttcca
     9901 gcaaaaaatc gaacaaatcg atataaaaaa agaaatatcc tatccttcaa agtacaaaaa
     9961 taataatatg gatatctaca caccgaaagt ggaaaagaaa aaattaccga ttcttttctg
    10021 gatgcatgga ggcgcatatg taggagggga taaaaatgat tgtcgtgatt atttggaata
    10081 tctatgttca gacacgcagc aaatcatcgt aaatatagat tatgaacgat caccggaagc
    10141 aaaacatcct acaccagtta tccaattgaa cgaagccatt caagcagcta aaaaagagat
    10201 aaaagatcaa gcagattggt cgaatatttc gattggtgga aattcggcag gtgcacaaat
    10261 tagtggagaa tatttgttag cacttcaaaa tgaagaaatt aaaaaagctg atcatcttga
    10321 tccgactttg gagaacaagc agattacgaa gtttatttct ttgtctggtc tgttggatcc
    10381 ttctgcactt actcaagtaa gtgataaaat ctctagcttt ctttatgaaa aatgtggatg
    10441 ggcttacttt gataataagg actttgaaca atcagagaag gtcaaaagtt tagccttggt
    10501 cagacatgcg gatcgttgga cgcaaaacac attcttaacg gatggaaata caaatacgtt
    10561 tacgaaacaa atggaagaag taacttctgt atttgagaaa gcgggggtga aagtcacaaa
    10621 agtagattat gcggagaaaa atgctgtttt gaaccatgaa tatcagtttg atttcagcaa
    10681 taaacaagcg caagaaacat atcaaaagtt agtaatgttt ttcaaggagt aaagtcttat
    10741 gatgcaaaag aggttgtaat ctgtcactta gctccaagtc acaaggagca atttcaataa
    10801 acaagctttt catggtttat gaatattgta acttgtgact gaggagttag agatggcaag
    10861 gggcaccttt tattcgaaat ttgggaatat cacacgatta ttgtcacgat ctcttttacg
    10921 taaattttat aagtcagatt tgttgttttg atcgtttaaa aaaagcaaca aatccgaagc
    10981 ctgcggaggc aagaagggac aaaaagccga gtggtaagca aaagaatgct ggttccacta
    11041 atattccgtt gttattgata taaggctggg aacaaagatg aatagcaaat aaaatcaatg
    11101 cactagcgaa aaaaagcaaa gaaatatgga tagctttcat aagcagcacc tcctttttta
    11161 taattgtatg ataaatagat taagaatcta taaatgtgac aagtttgtta ggatgtgaat
    11221 aaaatgatca tctagcgaac ttgaaacaaa gatggctaat gataagcaat taatgattca
    11281 gcaatcagat aggtacaaag tttaccatca tacgtctaga ccaataatag aaaatatggg
    11341 aatataatag aaaagattta ggataccccc aaaagaattt ccccaaaaat tcgatgtatc
    11401 ctaaactttt ttattttaca taacaatcat atcaactgtg aaataaaatt acaaatgata
    11461 acctaaaatg aggccaaaag ccagtgttat caaggaaata cttaattcac caaaattttt
    11521 tttaattttt tagaaaataa gaaacaaaaa taataaaaaa aagaaaagat aaatttttta
    11581 ctactgtata ctaactttgt tatcgcatct aacaagtaac tcgaataata ccaatacaac
    11641 cttctccaaa gatgcgatga catacgttct aacccaccga ctattgatgt tctgtaccac
    11701 ccttgcagaa ccaaaaaagc cactaccaaa tatttggtag tggctttttt tgttttatgg
    11761 atttttcgct ttttctgtta tggtgctatt ctttgatttc agaaatagta tgtcgaactt
    11821 tagcgataga acgacgtgat tctttttcgt tgtaaaacat caaaatcaca tatagagagt
    11881 ccaaaatgct tagttgagaa atacgggaag ctaaagcttc agatcggaat tctgtttctt
    11941 cagaaataga aataaaaacg acatcgccaa gtttagcaag ggaagaatta gcttggctag
    12001 taataacgat tgtttttgct ccagtttttc ttacggtttc agcaatatgg attgtttctt
    12061 ttgattgccc tgtgtgagaa atcaaaacag cacagtcttt ttccgtcaat aaagaagctt
    12121 ccatcaattg aatatggtaa tccgtgctgt gagaagttgc gataggggaa cgcaaaaatt
    12181 tatgataagc gtcagttgct acaatctctg aaccgccaac accgaaaaaa tagacacgtt
    12241 tagattcatt gataatagta gccgcttttt ttagatcctt ttgttgtaac aacttttttg
    12301 tgtcatttaa agttgaaata ctagactcga acaccttttg cgccatagtg agctcagtat
    12361 ctgttttctg aatattttcg tggatagaaa tagcagatag atcattttct tggatcaaca
    12421 tggccatttt aaaatcttta aatccattat aaccaagttt ttttgtaaat tgaaaaaaag
    12481 tagagtcagc aatgcccagt tcacttgcta aattgctaat aggactatga cttactttgc
    12541 ttggattttc taaaatataa tcggcgatag cctgttcttt tgggctgaag tcgttgtaca
    12601 ttgttttgat ttgtagtctt atggagacgc tcatgagaat tcctcatttt cataataaga
    12661 ttatcatttt ttccgttctc attaatgtta aagatacaag gagagtagga aaaaatcaaa
    12721 caaaaaatat ttttgtaaaa tctatttaca aaaaaatttt tttgtataat gagggtataa
    12781 gaaatggagg cgctatcatg gaaattggtg taattggttt gggaaaaatg ggattgaatt
    12841 tagctttaaa cttaaaagag caccatcaca aagtacaagg actcgacatt tcaagtacag
    12901 ctgttagtca ggcaagagaa aatgacattc ctgcttatca agagatctct gaatttctag
    12961 ctacttttga gcagaaaaaa atcatctggt taatgcttcc tgctggagag ataaccgaaa
    13021 acatgttaag aaaacttttt ccaaaactcg gaccaggaga cattattatt gaaggcggaa
    13081 actcttatta taaagattca atccgtcgag cagaagagtt tcagaaaaat gaaatcggtt
    13141 acttggattg tgggacttct ggaggaatcg aaggagcacg acatcaagcc tgcttaatga
    13201 ttggaggaga taaagaaact tttctatccg tggaacaatt attcaaagat gtagcaattg
    13261 aaagaggata tctgtatgct ggtgcatccg gaagcggaca cttcttgaaa atgatccata
    13321 atggaatcga atatggaatg atgcaagcaa tcggtgaagg attccaactt gtacaaaaaa
    13381 gtgagtatgc ttttgacttg gaacaagtcg cacgtgtttg gaatcatggt tcggtgattc
    13441 gcggctggct gatggaaatc atggaagatc agttaaacga acatcccaat ttaagtaatt
    13501 atcggggaat cgtttctgca tcaggtgaag caaaatggac ggttgaaaca gcgctagaaa
    13561 tggctgttcc agttccagta attgcaacta gtttgttcat gaggaatctt tcacaggaag
    13621 aagatagttt ttcagcaaaa gtagtttcag cactaagaaa tgggtttggc ggacatgaaa
    13681 tagttgaaaa gggtgagtaa aatgccagat tatcatatag caatcgttaa ttccagcagt
    13741 ttcggaagag tctttccaca ccacttggaa agattaaagt cagttggaga cgttcatttc
    13801 tttactgttg atcaagagat tccaggaaaa gaactagcgg agttacttaa aggattcaat
    13861 atcattattg caagtgttac gccatttttt cgacgagatt tttttgaaaa caaagatgaa
    13921 ctgctattga tttcccgaca tggtattggt tacaacaata ttgatttaaa agcagcaaaa
    13981 gaacaccaga caatcgtatc tatcatacct gctttagttg aacgaaatgc tgtagcagaa
    14041 aataatataa ctaatttgtt gtccatgctt cgacgcacga cacaagcaca agcaagagtc
    14101 aaggacgatc gatgggaaga tcgggcggag ttcgtcggta ggacgctgtt taacaaaaca
    14161 gttggtgtga ttggtgcagg aaataccgga agcggagtaa tcgaaatttt gcgtaacggt
    14221 tttcgctgtg atgttctagc ttatgatcct tacaaatcag atttatacat cgaaagcttt
    14281 ggtgcaaaaa aagtagaact tcaagaatta ctggaacaat cagatatcat ttgtttatgt
    14341 gcgaatttga cagaagagaa ctatcacatg atttctgata aagaagtcga tcagatgaaa
    14401 gaaaatgttt atctatctaa tagtgcaaga ggcgctctag ttgatgaaga agcgatgatt
    14461 aatggtctga aaagcggaaa gatcgcagga tatgccacag atgttttaga agaagaaccg
    14521 ggacgtcaag atcatccgta tttagcgttt gaacaagtag tgattacgcc acatatatcg
    14581 gcctacacaa gggaatgttt agaagcaatg ggtgaaaaat gtgtcagaga tgtcgaacag
    14641 atcagtcagc accttcttcc tgaacgttct gtccagacaa agagcaacta tataaaagga
    14701 tgatgaagaa aatggtgaac ggtttaaaag tgaaaacagg ccctcaattt tatttatatg
    14761 aagaaggtgg tatttcaaaa gtttctgatc tcttgaaatc atacggcgcg aaaagagtac
    14821 tggtcaccca cggaacggta tcatgggaaa aggcgctacc taaattagta tttttgaatg
    14881 acgaaacgat tcaatttttt tatcatcgtt attctggaga atgtagttat gctgaagcca
    14941 gacgaatagc tacaatcatc aaaaaaatga aatcgatttc gtagttggcg ttggcggagg
    15001 aaaactgacc gatctagtgg gttatagtgc caatcttgca agaatcgatt tcggagtcat
    15061 ccctacttta gctagtaatt gtgctccatg gacacctctt gctgtcatgt ataaggagaa
    15121 tggacaagca gaagggaaaa cagaacactt ttacagacaa gctgcttttt ttataacaga
    15181 tccccaatta gtgattgatt cccctgttcc atatttcatt gccgggttag cagatacact
    15241 agcaaaatgg tatgaatctg agctcatctt agaacaagat tttttgcaag atgaatcatt
    15301 tttgaagcta gcacaagata ctgcgaagat ctgcaaagaa gagattttga atcattctgc
    15361 taaagcaatc caagatatgg cacaaagaaa actgacatcg gagttcaaac agctttcaga
    15421 gattatcttt gctgttgctg gtttagttgg aggattagga gataaatacg caagaaatgc
    15481 ggcagcacat gctatgcatg acgctatcag taaatatatt ccggaaagcc atcgttttct
    15541 acatggagaa aaagtagcgt atgggatttt ctatcaactg gcactagaag gtcgctggga
    15601 aaaaatagac gagttatatc ctttgtatcg ggaattagaa ctgccggttt ctctagaaca
    15661 aatgggatta tttccgaaag ataaacagat aatagataaa atggctgaat ttattgattc
    15721 gaaagagaaa gtgcatttga ttcctataga ggtaacacag aaatcactga tacagtcaat
    15781 gatggaactg gaaggttata tcagaaagat ctgaaggagg aggacacatg gaaacattaa
    15841 gtgtagtaca aagtttgttg attgctttat gggtagccgc ggttatgtcc cgctggttag
    15901 gtggaggagc cacgttgacc ttacgttttt ctcctttgat gactggatta gtcgtcgggt
    15961 tgatcatggg ggaagttgca caagcgatga ttgttacagc cgctttgcaa atgatttata
    16021 tgggggtttt ttctccagga ggatcaatgc cagctgaacc atctattgca gcagctatcg
    16081 cagtaccagt cgcattatta ggggatctgt cgccagaagc agcgattgcc gttgcggtgc
    16141 cagtcggatt gctcggaagt tacttgtatc agtttcgttt tttcatcaat acttttttag
    16201 gtaaatatac agacaaagct gtagaagatt tgaatacaaa agctatggcc cgatcaatta
    16261 ttctttatcc aacggttgcc tcatttttat tattcgttcc gcttgttttc attgctttat
    16321 atttaggggc gccattgatc gctgatgtaa tcaaagcttt agagggaaca gtcgttatcc
    16381 acattcttga agtagtaggt ggagggttag cggcaatcgg tatcgcgaca actgtatacg
    16441 ttatcggtcg aaaagattat ctggtctttt tcttccttgc ttattttatg agtgttgttt
    16501 tgaaatcttt agatattacg atggttactt atgccatttt tgggattatt atcgctttga
    16561 tctttgtcca gtcgcaaaaa gggaaacaag tacagcctgc cacaagcaat aacaattcag
    16621 catcttttga tgacgaagat gactatgatg acggatttta atttaggagg aatgggaaat
    16681 ggctgaaata atggataatc aaacaggtgc tcctgaagaa atcacgaaaa aagatgtaac
    16741 aaaagcctat ctgagatggc attttgcaaa cgaaattcct cactcttttg aacgttactt
    16801 ggcgccatca ctactatacg gaatgatgcc aatcttgaaa aaactttata aagatgaaga
    16861 ctctttaaaa gcagcatata aacggcagtt actgttcttc aacactcaat taagctgggg
    16921 tggcggtgtg attactggcc taatgtcttc tatggaacag caaagagcag aagaagagta
    16981 tgaagaaaaa gagatcttga tgcaggatga cttgatgtat aacacgaaag cgggattgat
    17041 gggggcacta gcaggaatcg gagatgcaat cgattcagga accgtccaat atatttttat
    17101 cgcaatcgcg gtaccttggg cacaacaggg aagtccttta ggcgcacttt ttccttttat
    17161 ctgctttggg ttgtatcagg ttttactagg ggtatttttc gcaagacaag ctttcactat
    17221 gggccgtaat gctacaggcc taatgcaaaa cactggtgtt cagacagcga ttgaaatgct
    17281 atcagtttta gggttgttta tgatgggggt gcttgcggga aactatgtaa aagtagaatc
    17341 ttcattgaaa tttgcgatat ccggacgtga gtttgttatc caggaaattc ttgatcaaat
    17401 cgtgccaggg atattacctt tagctgtcgt aatgggtgtg tacttgttct atacgaaaaa
    17461 aggattgaaa gtcacacaag cattattatg gctgactgga atattaattg tgctagcagg
    17521 aatcggtatt ttataaaaag aagggaacgt gaataagatg ggtgtagtca atttagcgcg
    17581 tgtggatgag cgtttgatcc atgggcaagt aatggtaacg ttatctcaaa aaagtggagt
    17641 gaattccatt tttgttgtag acgaagttgt agcaaaagat aaatttatga gagatcttta
    17701 caaaaatgca ggaagccgta caggacaaaa aacaattgtc attacaccag agaaagctaa
    17761 attctattgg gatgaatatc aatttaaaga atacaattgt attttgatcg caaaaacagt
    17821 ccaaacgatt tacgacttag taaaacacgg aatccccatg gaagaattga atattggagg
    17881 gattgctcaa aaaaatcctg atacagatct tttagtgacg aaatctgttt atttaaataa
    17941 atcagatgct gaaaaattga aagaaatgaa tgaacaatat ggcgtaaaga atatttattt
    18001 tcaagccact ccatctgcac ctaaaacagg attaaaagat gtattgaaca agtttgattt
    18061 gtcataaaca aatgcttaga aaaaagataa agagggcgaa aaaatgaaga agaacgagct
    18121 gtttcgtgat tgggaatttc gttatcgcta tatttaccgt aagcgccgta caaaaaagag
    18181 taaacagcgt tttctttcag cattagtatc agatatttac agtatgagaa cagatgtgac
    18241 agtcattgct tacgatacgc tagcctaccg atcaaaaaat atttatgttg gagatatcga
    18301 aaaagcagag aaagttatct gtacttatta cgatacaccc gttcatgcat taggaagtta
    18361 tttcatgttt gattggaaag accaaaggaa aaaaacaatc tactctatat tattgtcctt
    18421 cattttgttg ttcagtttag gttggtgggg aatgatgata tataacaaaa atcctcatca
    18481 cgtattcgat ctgctatccg tataaacagg tatcactgta ctagcatttg ggagctactt
    18541 tttcttattg ggaaaagcag ctcgcggatg gcctagcaga caaactttta ttcgaaatac
    18601 ttcatccata ttaacgatgc tagaaatgat ccgtacgatt gatgatccga gtgtagcgta
    18661 tgcgtttgtt gatgaaggct gttatggaga aaagggctta gacagcgtga gatcgggtat
    18721 gaaaaaagag gctattcttt tttatctaga ctctgttggg gcagatacac cgctacagtt
    18781 ttcgggaaat tatttttcaa ataaagaaca gtggctgaaa caagttgata agttgaaaga
    18841 aaaaaacgtc aattatattt ttagtgcaag aaaaaagcaa gcgcagttct tctatttgac
    18901 caaaacagat ttacgaggga aaacatttaa ttggcaaaat gccaatcaga ttattgctct
    18961 ttttcgataa gtaaaaagtg caaaacaagg agtgagaaga ctaatgttgg aaatcatgat
    19021 cgttacacat ggtaagttga gcgaaggact gaaagatgcg gcggaagtaa ttgtcgggac
    19081 atcgagccat attgaaacaa tgtgcttaga gtctggggag gacatccaaa cattcggaga
    19141 gaaggtcaaa gaaaaattaa ctatgatcaa cacagcagaa cccatgctag tatttgttga
    19201 tctaattagc gcaagtccgt acaatcaagc gctcttggca gtcaatgaac tgactgaaga
    19261 aaagaaagca gccacttatg tcataggggg cgtgaatctg ccaatgttgt tagaagcaat
    19321 caatcatcag atgcttcaca caccgataga agaagcggtg gaacaaattg tcaaacaagg
    19381 aaaagataat ttaactgttt ggcatgcttc agcggaagaa aaaaaattag atgaggacga
    19441 tttttagaag gaaggctgaa aagatgaagt ggggatttag atggtatggt gaaaaagggg
    19501 attcgatccc attaaaatat gttcgccaaa taccgggaat aaatgggata attggaactt
    19561 tgctgactaa aatgcctgga gatgtttggg agatttcaga aattgaggag ttgaaagcat
    19621 ctgttgagag ccaaggactg gaactgcttg gaattgaaag tgtagcgatc cacgatgcaa
    19681 tcaaggcagg gacagatgaa cgagattact atattgataa ctatatacga acaatcaaaa
    19741 acttgtctcg atgtggggtt cgattgattt gttatagctt caaaccaatt tttggatggg
    19801 cgaaaactga tttgtttaat gaaaacaacg atggaagcta ttctctttta tacgatcaga
    19861 aaatcgtcga tcagatggag ccaggagaaa tgtatcaatt gatacacagc cagtcaaagg
    19921 gattcaaatt gccgggttgg gaagaagaac ggttgaaaaa attcgctcag ctggaaaaac
    19981 tgtatgaagg aatgaccgaa gaagaattat ttgaaaatct gacttatttt ttagaaaaag
    20041 tgattccagt gtgcgaaaaa gcagatgtaa aaatggctat ccatccagat gatccgccat
    20101 gggaactttt tggttttcct agagtgacga aaaatcttgg cgacttgaag aaaattctaa
    20161 atgcagtaga ttctccatat aatggactaa cgctgtgcac aggatcttta ggggcaaatc
    20221 cggaaaacga tttagttgaa atcatccatg aggtaggttc gcgcatccat tttgtccact
    20281 tcagaaacgt ggagttttta ggagaaagaa agttcaaaga aacggcacat ccctctacag
    20341 aaggctccct tgatatgtat catattatga aagcgttggt gcaagaaggg ttcgacggtc
    20401 ctatacgccc tgatcatggt agaaccgtgt ggggtgaagt ggctatgcct ggatatggcc
    20461 tgtatgatcg tgcaatggga ctcacttaca tgcaaggtct atatgaggcg ataagtaagg
    20521 aaaaaccact gggagagcat tatgaataaa atcaagctct tcatctttga tatggatggc
    20581 cttttattag aaactggacg tttagcttat cgtgcttatg taaaatcagc acaaaagtat
    20641 gattatgaaa tgcgtaagga agtctattat ttactaactg gccaaacaga aatggcgata
    20701 agagaacaaa tgggcatttt gtacggagaa gatgttccat atatcaaatg gcgagaagca
    20761 atcaatcaat ataaagaaaa aatcgtgaaa gaagataaga gagtatatac gaaaaaagga
    20821 gcagaagaaa ttttatcttt tgcaaaagaa agaggaatcc atacaatcgt ggcttcgtct
    20881 aatacacggg taaaagtaga aatgtattta aaaatggaaa atctatatgc cttgtttgat
    20941 caaatcattt caggggatga tgtaaaaaaa ggaaagccgg aaccagagat ctttttgaaa
    21001 gcttgttcaa aaatgaatat tcctccaagt gaagcacttg tatttgaaga ctcgattgca
    21061 gggatcgaag cagcaaggcg agcgggcatt ttgtcttttc tcatcaaaga tcatttgata
    21121 gggttgcccg atcataatgg gaagcataag ttgaaagtag atgtaagttt atatgaatcc
    21181 aataaagcag actatgaatt ttatgacctg catcaagcaa aacgattttt agaaagtaaa
    21241 gggttgtatc tctaataaag aggactattt tctagtcctc tttatttatt gtcctgcacg
    21301 actgagggaa cagatgaaaa agaagtaatt ttttattttg aagtatctac cttatttgta
    21361 gtgaacaatt tagaacaaac acaaaacgca tctactgtta tctaccagta gatgcgttta
    21421 aagagaagaa cgagaatcgt ccatatgagg gtttccactc cttatgcaga tttttttaat
    21481 tcttgcactg cttgttcgat cgtaacacct gtcgctacat gattggtatt ttctttgtca
    21541 aaaacgacaa atagattcaa atctgtatcc aaggttactc gatattgtat atctttgtta
    21601 tagaatgtca tagccattct cctttcttta aatacttgct gaaaggcaac gaacgcgttg
    21661 ataacgaaat tccatttata agtataccac caattgaaag gttttcatag ttaagaaatt
    21721 ttaaagtcaa aagttgcaga taaaagcaaa taaacaagaa aacaaaaagt gaatcaatcg
    21781 cggttattca actttttctt cgattcgaag acaatagaag acaatgaatt ttatatttca
    21841 ttgcattatc cttgtgttat tagaaaaagc atgatatatt aaatgagtaa tctattttga
    21901 aacgtaattt gagcgatata tctacataga taaaactcct tttgcctatc aaatatttat
    21961 tgcaacgaaa aaacgtacat tgatacgtat caggaggaat ttatttatga ataacggtac
    22021 agtaaaatgg ttcaacgcag acaaaggttt tggctttatc actggtgaag atggaaatga
    22081 tgtatttgca catttttcag ctatccaagg tgacggcttc aaaactttag aagaaggcca
    22141 agcagtgtct tacgatgttg aagatggtca acgtggccct caagcaacaa acattgttaa
    22201 agcttaataa aaattgactt ttaaccgtct tttcgaaaga aaagacggtt tttttgtacg
    22261 aaaaaatt
//
LOCUS       DACDXO010000045        22052 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E23605_2017
            SAMN12639989-rid9881463.denovo.045, whole genome shotgun sequence.
ACCESSION   DACDXO010000045 DACDXO010000000
VERSION     DACDXO010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639989
            Sequence Read Archive: SRR10023389
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22052)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22052)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741914.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:42:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,972
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 33 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 17 of 73
            Pseudo Genes (multiple problems)  :: 10 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22052
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639989-rid9881463.denovo.045"
                     /isolate="E23605_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(375..689)
                     /locus_tag="IWM35_001877"
     CDS             complement(375..689)
                     /locus_tag="IWM35_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9919724.1"
                     /translation="MDFYSNFILIIAILLLLNIWFFDKSRNAGIGFRTKRSTSSEKKW
                     VYSQTIFYGGVISISLLSSTLYSFNVIDVSMSNFISIIGILISAIITQLLLVFEEKSK
                     NN"
     gene            975..1214
                     /locus_tag="IWM35_001878"
     CDS             975..1214
                     /locus_tag="IWM35_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9919725.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
     gene            complement(1576..1836)
                     /locus_tag="IWM35_001879"
     CDS             complement(1576..1836)
                     /locus_tag="IWM35_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9919726.1"
                     /translation="MNNETIILGKKYICQPIGLKQKVVGEVISKLENCVVICVDEYHD
                     VDRNEILEKCGKVVAKYTHVYELETTEYLYNYSNKYEHTLVS"
     gene            complement(2021..2518)
                     /locus_tag="IWM35_001880"
     CDS             complement(2021..2518)
                     /locus_tag="IWM35_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HAP9919727.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQAFLEKYVDLLLANFNINAF
                     SFINN"
     gene            complement(2648..3358)
                     /gene="budA"
                     /locus_tag="IWM35_001881"
     CDS             complement(2648..3358)
                     /gene="budA"
                     /locus_tag="IWM35_001881"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HAP9919728.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNIQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            complement(3371..5035)
                     /gene="alsS"
                     /locus_tag="IWM35_001882"
     CDS             complement(3371..5035)
                     /gene="alsS"
                     /locus_tag="IWM35_001882"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HAP9919729.1"
                     /translation="MAEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVEKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAKLTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
     gene            complement(5243..5905)
                     /locus_tag="IWM35_001883"
     CDS             complement(5243..5905)
                     /locus_tag="IWM35_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAP9919730.1"
                     /translation="MKEQRFDKNVYMEFFYWLYQSLASSLCFWCVNILYIAAAFLLSI
                     DGRNVFSFIFSLLFIGPGCIVMIGMTEELAKENSFSPVRLFIKMFRQFWLKGFFYWLF
                     AWIVSVIMIFDCFFFIRFSYGKWLIPLFVLLACLSVSFSTNCWYFQVRNPASKPNQVL
                     RIAFYYTLKKWYVSLLDFLLLTSLFLFFFVKPQWCILLGPSIVFGLIYFNNRKLMRTM
                     DL"
     gene            complement(5915..6730)
                     /locus_tag="IWM35_001884"
     CDS             complement(5915..6730)
                     /locus_tag="IWM35_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP9919731.1"
                     /translation="MPFIQWLGMILLIVLALLTIIPFLWMVSASFKANNEVFTIPIQW
                     IPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFTSSFAAYGFSKLNFKGRDI
                     LFLAYIGTIAVPWQSYMIPQFIMMRKVGLTDTLWSLVLLQAFNAFGVFLLKQYYSSIP
                     DSLCESARIDGLSEWGIYRKIILPLTKPAIASLTIITFVNTWNDYMGPFIYLSSTENK
                     TIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQKYFVEGIASSGMKE"
     gene            complement(6991..7434)
                     /locus_tag="IWM35_001885"
     CDS             complement(6991..7434)
                     /locus_tag="IWM35_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HAP9919732.1"
                     /translation="MLDPVDTEKYETILLPRKEKWGLLILENSVLAKKDVITKEDFAN
                     VPLLISKRPEIQRMFADWLGIKKEQLLITGTYNLIFNVFALIENQVGAALVIEGAVSH
                     HSKRVTFVPLDPMIETNCMLVWKKNRILSPVVNELIHRFKEAFEQ"
     gene            complement(7465..7578)
                     /locus_tag="IWM35_001886"
     CDS             complement(7465..7578)
                     /locus_tag="IWM35_001886"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP9919733.1"
                     /translation="MEADSSNTLALLLEKLIRDYPSVQFHIHSGTSDDITE"
     gene            complement(7568..7906)
                     /locus_tag="IWM35_001887"
                     /pseudo
     CDS             complement(7568..7906)
                     /locus_tag="IWM35_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321036.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(7894..8271)
                     /locus_tag="IWM35_001888"
     CDS             complement(7894..8271)
                     /locus_tag="IWM35_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP9919734.1"
                     /translation="MNIKQVSEEKGISADTLRYYERIGLIPPVNRTKGGIRDYTEEDL
                     KWVDFTICMRNAGLSIESLIEYIRLSSEGEATVFERRKLLIEESELLTKKIAEMQECL
                     ERLQGKIKKYDRVLSRNKMECIK"
     gene            8495..9688
                     /locus_tag="IWM35_001889"
     CDS             8495..9688
                     /locus_tag="IWM35_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP9919735.1"
                     /translation="MTTYQRKVLVSTSAGIALENMDIMFLAFSLSSMITSFHISGTQA
                     GFISTITNLGMLIGGIFFGIIADKYGRVKVFSQTVLLFSIASLLMYFASNIYLVYLFR
                     FIAGVGAGGEYGACMSLISESFSKKQIGRASSVAGIGAQVGAALAAILAAVIIPTFGW
                     KMLYVVGVLPVLMVLFIRRGLTEPKEFKSVQSAEKKTKLSRLFESKHLAWQTCGLSIM
                     VTVQIAGYFGLMNWLPSIMQNRLGLSVSGSSLWMVSTILGMSLGMMTFGIIMDKLGPK
                     LAFSLFLICSSLSVYLLVLAHSQWSLVAAAVIVGYFINGMYGGYGAVISSLYPTEIRA
                     TANNFVMNLGRAVGGFSSIVIGFLMDHYSLTAVILFLSGIYLVSLLVLTTLTGVTALR
                     ESLQH"
     gene            complement(9854..10276)
                     /locus_tag="IWM35_001890"
     CDS             complement(9854..10276)
                     /locus_tag="IWM35_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP9919736.1"
                     /translation="MQTFLTKVSQKKIKMIHLLLETERWCSIEELQNELKVSSKSILN
                     YLTDLEELFQQYPDKVILKNENNRRFSMEKQENFPIYIIYLHFYRKSYNYHLIEFMYQ
                     HPEKNLEDYADAQYTSVSTVFRYAKLLVKYFEMTDVKE"
     gene            10859..11353
                     /locus_tag="IWM35_001891"
     CDS             10859..11353
                     /locus_tag="IWM35_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9919737.1"
                     /translation="MTEAESALSQAEANPTKENYATASAAIQALPENKQELTSRLAAI
                     DSTIKAKEAEEERKKQEEIARQQAEQAQQEAIAQQQAEQARQEAIAQQQAEQARQQAA
                     AEQASQQAQAAAPEQNNEQTVYVTPTGSKYHTHKCGNGTYTPATLSEAQARGLTPCSK
                     CYGN"
     gene            complement(11515..12687)
                     /locus_tag="IWM35_001892"
     CDS             complement(11515..12687)
                     /locus_tag="IWM35_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP9919738.1"
                     /translation="MNKSRIRILDIFMAIILVVGIGIFSYPFVEDSLNDFLAQQMIIH
                     YQKQASNKNSEEIKKQQEKMTKKNQQLAEQNVSPGIGSFNQAVDAKALKDLPSNAFFM
                     EHMLGVIEIPKINVSLPIFDQTTEIFLQKGTSLLEGSSYPTGGKNTHAVLSGHRGLPE
                     AKLFTDLPKLKKGDQFFIQINGKTLAYQVEKIQVVLPDEVDSLGIQKGRDLVTLLTCT
                     PYMVNTHRLLVTGHRIPYQAKEAKKAIQGIDQWKKWKFFIWFIGILLGSIGLVWLLIA
                     YLDSLAIAKRNYPLSFYVKNTNGRPIEGMVFSVKTLNGKHYITREKVPFVKASDEYGL
                     VRFSDLKGRNYRLQHEELLLKIHVKHKHSKQFSMKLKKGRYKLRKEKEVYYLIEKE"
     gene            complement(12893..13708)
                     /locus_tag="IWM35_001893"
     CDS             complement(12893..13708)
                     /locus_tag="IWM35_001893"
                     /inference="COORDINATES: protein motif:HMM:NF033902.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAP9919739.1"
                     /translation="MTGFDENTGVALYDPAESALTFTTDTEGSISVNGLIDGNYTLVE
                     TKAPDGYQLTNPQKETTFIVKNGKLQQTTGIIHGADEANLGNPNGVVNRENTTTDNTI
                     TVENVQNYGDFHFIKQDVNTQEKLADAEFSILRAKDPTVKLMKKIANLASGEYPYAWS
                     DEVTGSDWEEVKLISDSEGKFSIEGLKQGAYCLQETKAPEGYALPKNEAAYTEFTVDD
                     DPTTSDETTIDNQPKGILPSTGGMGIIVFILVGTALVGGAVIYFKKRHQETEA"
     gene            complement(13858..14304)
                     /locus_tag="IWM35_001894"
     CDS             complement(13858..14304)
                     /locus_tag="IWM35_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HAP9919740.1"
                     /translation="MKNIKTKIWTFLGTAIMLLPFVLGLGTAEVSAAVSPTPENVTVN
                     LHKLKFTSAPENQINNGTELTFPNSEPLNGVEFNVYDITATYYPSKDTAVPADATPFA
                     SVTTSGEGLANLTLPGKSDGKDAVYVFVETPKPGVETSPNIVLSLP"
     gene            complement(14301..14936)
                     /locus_tag="IWM35_001895"
     CDS             complement(14301..14936)
                     /locus_tag="IWM35_001895"
                     /inference="COORDINATES: protein motif:HMM:NF037639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP9919741.1"
                     /translation="MTLKENSEKTTFDNDVRLTTDNQTVETHLAIQTGGKQFVKVDRQ
                     TKQKLADAQFLVKKNDQYLAQENGINHWVAKDDSRATIFTSSKDGTFSVHGLSFGSYE
                     LVEIKPPKGYILSQSTILFEVEKGSYDPLHPIPLEIINEPKANPTNNGKTPVQMQTNG
                     PSNEKNGGSFPKTSEEMLGGFSILGLLLVLSTGTAWFYKKQQKGNNRREIK"
     gene            complement(14914..15306)
                     /locus_tag="IWM35_001896"
     CDS             complement(14914..15306)
                     /locus_tag="IWM35_001896"
                     /inference="COORDINATES: protein motif:HMM:TIGR04226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAP9919742.1"
                     /translation="MTDSSNHKLTTIHLYPKSEQKIPAALTLTKTVENKQTDFADGDK
                     VPYLITVTIPENINEIGTYTIKDTADPQLWLEPETIDFLIGGDKIHTFHTQKIKHGFV
                     LSDSGKDLSSFAGKKINDPLPNDIERKQ"
     gene            complement(15346..15675)
                     /locus_tag="IWM35_001897"
     CDS             complement(15346..15675)
                     /locus_tag="IWM35_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HAP9919743.1"
                     /translation="MFESDKMPSISQNDGINIPDNFQGVKGLNGVTFAIYDVSDEFYK
                     LRSEGSSVEDAQRKLAQKSDSEKILAENVTKTVDEEEGIASFSASDKDEQGRDAVYRF
                     TEIKTSD"
     gene            complement(15801..18128)
                     /locus_tag="IWM35_001898"
     CDS             complement(15801..18128)
                     /locus_tag="IWM35_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP9919744.1"
                     /translation="MKMIKKTQNKIQRICQKHFVSRLKAVSKRKYLHPIMISIILIFT
                     FLGSLSSIYVNVYTETSNFANQTTEETNSPPYSEIQSIESTNQSSNNETTSTTSSTQL
                     IDPTEKEITAAKAAGEKAYSETGRSQTFSTVAATANFNGHTIPDGYTVIYAKDGVIVA
                     VNGSMSADKTALQGVTKAIAFIDNRPDIHNLFIGSTVNGTTITFPKSIYMGVATAGNT
                     IDSVTPNADKSYSFTPSTWGGLPAGGGMSTFSIGIQLANDIPFTGYSIKNGQIVDGKG
                     NVSNFPTYQAATVQVNANRTGTFSYTVNGGAAKIGTYGTTLSGIHVGDKVVITPTAVT
                     GYSFAQTNGSFTALESNVDTVTYTGKQTPITIKYQDYFGQMIAPSKIMNLTYGSAAQD
                     LTTNTPTINGYTFTAVSAIETKNQSAMSVLASLDTNGNVIVKDANGKQISEVILYYKT
                     NATVSINANGSKYYDGLSVVPIVKYAFNDKTESTSLLNNDLSIDQITWNPTDFKAMNE
                     KGVEVTAPTEIGTYTSWQLTKSGLAKLAARTNYLFTIVQTSDVYTIKQISGAVELSGS
                     KTYDGKAGVPSIHVKLAEGVTSSLTPVQVALSSMDYTADAQSAKNMVNAGSYTIKLTN
                     SGIDKVKAANPKLSFTDIANTSGTYTIYKANAVITVDDAAFNYDAQSHSISVGNLHVT
                     GVVLGESLDYTLTVNSRTDIGSQIVTISLGEGSVNNNYTIIATDTAKLTINPTLLLPS
                     TGGIGLLPFVFLGLIFIFSGFFYFIHRKKKAGEQR"
     gene            complement(18971..19264)
                     /locus_tag="IWM35_001899"
     CDS             complement(18971..19264)
                     /locus_tag="IWM35_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HAP9919745.1"
                     /translation="MVYTYTEQEKMKVLDTFMNDGKVKQVPSSTKKKYILLKEVLKRF
                     DHGVTYTETEVNSILLNVFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
     gene            complement(19667..20845)
                     /locus_tag="IWM35_001900"
     CDS             complement(19667..20845)
                     /locus_tag="IWM35_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP9919746.1"
                     /translation="MEKTDRFKKQISSTGRITWYFQAYRTTRRGFNSKREAQLAYLEM
                     EKQQRLKKKIVASNDKFSVVGEQWFQYYRSLNEQKEITYDKREEQLKIIVRWIGDKKL
                     SDLTPECLQELLFTLKECGVNGTNVGYAKNSLQSLIQVLNMIFKYCQRKNLISENPMK
                     SVRMPKYQLTVTDLKEAINNIEDKFLTVDELRTFLNYGIVHEELPMSVLFHVLFYTGC
                     RVSEALALQPGDIDFKRNEILFYKQTAVKGKSKDFRIETTKTVSSARRVLVTPLVMEK
                     LQQLIKALGEMRQHYRFIIEETYLFVYLDSSKRGVPYRREYVNDHVKRCVNRSGINKP
                     FHTHLARHTMASLVAEHCSWEVLKDRLGHTDQTTSKIYRHLTSNEKVKPLIAFSSLES
                     "
     gene            complement(20949..21263)
                     /locus_tag="IWM35_001901"
     CDS             complement(20949..21263)
                     /locus_tag="IWM35_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9919747.1"
                     /translation="MELEVKTNVNSLEIEFYSILQKKLEDYTEKLLAVSHLPLVLTNT
                     DLKRLFQISDSTLNRLVKLGEFPPCWYGIRGHYFRDDVLEWMRKSDSNDYREKLRLLR
                     SL"
     gene            complement(21583..21978)
                     /locus_tag="IWM35_001902"
                     /pseudo
     CDS             complement(21583..21978)
                     /locus_tag="IWM35_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317574.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
BASE COUNT         6554 a         4300 c         3711 g         7487 t
ORIGIN      
        1 ttttggtcaa ggagagaaaa gtagagaaat acaaaatttt ttgcattgat tttgcattca
       61 aaaaaacatt caaacattta gaaatcccca atacgtattc aggaaaatct attttaaggt
      121 ttacaaaaag aacttttgtt cgtacactct ttttgaggtg aacttttatg ctgatggaag
      181 gaaaaacaca attatggttt aagcatgttc cttcaaataa gttttttaat tataaactct
      241 agagaaatta attcatccta acataaaata attgttcact caaaagtttt tctagaacaa
      301 ttaaaacagc aggcttcaca ttcgctatac atttgtaaga agtaataatt cctgtactaa
      361 aaggcataat tgctttaatt attttttgac ttctcttcaa acacaagaag tagttgtgta
      421 ataattgcag aaataaggat tccaataata gatataaaat ttgacatgga aacgtctata
      481 acattaaaag aataaagtgt tgaagaaagt aaactgattg aaataacccc tccgtaaaaa
      541 atagtctgtg aatataccca ctttttttca gaagacgtac ttcgtttggt tctaaaacct
      601 atccctgcat ttctactctt atcaaaaaac caaatattta gaagtaataa tattgctatt
      661 attaagataa aattactgta aaaatccaaa agctatcctc ctagcaacta tttaaatacc
      721 caaaaaaatt acctctagat aatatcatac ctacgttttc acttataaat tttttcataa
      781 aaaaagcgtt tagaggcata atcgttaata cagatggtaa ccaatgtatc tggtttgaac
      841 tgggatatca ccctctccat atatgtatta ctaagactaa tagatactat aaaagacttg
      901 atagttttgc attgattttg cctaatactg agcataggtg tatgctaaac gatactataa
      961 ggagtgtgta cataatgaaa agaattctgt ttatcagttt atcaaattta ttgttactta
     1021 ctgcttgtgg gacttctgca tcaaagaaca taccttcaga tacgcaaatt tcgaatagta
     1081 tcgattcaac aatttctaca aatgagaaaa attctccggt agaaacttcc gatataaaag
     1141 tttctacctc tgcaaaaaac agggtaaaga aactgtcagc acgtctcctt tatttcaaac
     1201 aaactctgaa ttagcttctt catcttcttc agcaaataat gatgatagtt tcgcgtttac
     1261 tcctaactaa atgcaacctt tacaaatgct ttacgataac aagcctgagc taaatgatcc
     1321 tgatatcagt tttgcatttt ttgaaatgat tggatcggat gatcttttta aaactactag
     1381 tttgtctatc cgaaaatagg acagttctgg cagtataggt ttttatcgag tttctccacc
     1441 aggcctcgtt acagagacag attctctagg cgaacctttt gaataaatca aaaatcatga
     1501 acaatcactg ccaactaaat aaagtgcctt caaaagcttg gtcttctcgc ttttgaaggc
     1561 actttattta aatctttatg aaactaaagt atgttcatat ttgttgctat agttatatag
     1621 atattctgtt gtttccaact cataaacgtg agtatatttt gccactactt ttccgcattt
     1681 ttctaatatt tcattgcgat cgacgtcatg atattcgtct acgcagatca caacacaatt
     1741 ttctaattta ctgattactt ctccaaccac tttttgtttc aagccaatag gttggcaaat
     1801 atattttttt cctaaaatga tagtttcgtt attcatattt catccatcct taatgttttt
     1861 tcattattca aaatgataat accattaaaa tatgattgtg aaaatcaata acacttgtga
     1921 ttttataaaa aaataaaaaa accgaaaaac agaaatgacc actgtctttc cggctctttt
     1981 tattcttgat ttctcttctt ttttgtttca atagacgatt ttagttatta ataaatgaaa
     2041 aagcatttat attaaaattt gctaataaaa gatctacata tttttctaga aatgcttgat
     2101 cgatttcatc ataccctttc gttactccgc aatcgatatc taaaacaccg attaaattac
     2161 cttcttttac cattggcact acgatttctg attgtgcggc tgaatcgcag gaaatgtaat
     2221 ttgcatgggt tttgacatca tcaacgatca atgtttcttg tttttcagct gattctccac
     2281 aaactccttt tcccatcttg atacgtgtgc aagaaactcg cccttggaac ggtcctaaaa
     2341 tcaattcatt accatcgaat aaataatatc ctgcaaagac agtctcaggc aaagtctcat
     2401 tcaacaatgc agatgaattt gctaaatttg ctaaccagtt atcttctgct tctaataatc
     2461 cttgttgctg taataataaa atttcgtatg catctttttt ctgtttttct gtccacatga
     2521 taaaaaccgc tcctttttga tctatggaga agattatagc tcagtttttt tgtaaacgct
     2581 aggtttcctc ctcaaaaaaa cgagatacgt caggttaatc accttgaacg catctcgctg
     2641 tctttttcta ttctgcttgt tggatttctg ctgctaaatc agcataatca atcgtttcgt
     2701 gaacaaaagt cggatcatca actggaaaat gctgtacaag ggacgagatg cggctaatct
     2761 caacttttcc ttcagccact tcaaaatcca ttacatgtcc gccaaatgta tgtgtatcat
     2821 cgataaaatg caggtgaaat cccgctgcag caactccttg gaataattca ggcgtaaaga
     2881 acccgactaa tgttccttgg atattgcttt gttggaattc tggttgagac tctgaaattt
     2941 ttgctagtct tctgtaaggt ttttcttgct tcggcatgat acggatgtgc atatttttga
     3001 ataatccagt tatcttcact gccaaaaata ggttttcccc ggcttctctt actaatatgt
     3061 cttttttcaa ttgttctcca tctgcagttt ttgagacaga aaaactagta tctggcgtaa
     3121 aatctgtaat tgctgcatag ggggtaagtt cacttccatc taattgggca actgagccat
     3181 ctgatcttcc ttgataagct acgccatcta aaaagatgac ttcaccatca agcccatgca
     3241 atgttcccaa tcccagagtt cctttttcta atatatgagc aatcgtttct gttccatcca
     3301 tcaagccggc catcaaagct cctaatgtcc cgtgctggta taagattttt tcagtcatgt
     3361 cgacacttcc ttaatagaat tgatctggta agattgtttc tcccaatttc tcattatcac
     3421 ggtaatcaat cggaatatca ataattacgg gtccttctgt cgcaaatcct tcttctaaag
     3481 ctttttctaa ttcctctaca cttgtagctc ggattccttt agcgccaaaa gcttctgcat
     3541 atttcacaaa atcaactgga ccaaagtcta cacctgacgc ccgctgatat ttcatttttt
     3601 cttggaactc taccatattg tagtgtccat cattccaaat caaatgaacg atattcaatt
     3661 ttttacgtac cgccgtttct aaatcctggg cagagaataa aaatccccca tcgccggaaa
     3721 ctgatacgat ttgtgtattt ggacgcacca atgcagcaga aatcgcccat ggcaatgcaa
     3781 caccaagcgt ttgcatacca ttactgaata acaaatgacg aggttcataa cttctaaagt
     3841 ggcgtgccat ccaaatataa tgactaccta catctacggt aacagtcata tcatctgtta
     3901 cttttgactg aagcgtgtta ataacttcta aaggatgcaa gatacctgct tctccttttg
     3961 attgaacaat gtcccgctcc gttaattttg cttgaagaga tgctaaatat tcttttgcat
     4021 cttctgacac ttgttgaggc tctaatgaac ctgtcaataa atcaagagtg gctgagatat
     4081 caccgatcaa ttctctttca ggctgcataa aaggatcgat ttcagctggc gcttcatcga
     4141 tcacaatgat ccgcgcatct ttttccgcat tccagtttct tgcttcatat tcgattggat
     4201 catagccaat tgcaataact agatcacttc gtttcaaaag catatctcct ggttggtttc
     4261 taaataaacc cacacgacca aagaaatgcg cttctaattc acgtgagatc actccagctg
     4321 cttggaatgt ctctacaaca ggcaatgctg tcttttcaac taattgcctg atggctaacg
     4381 tttctttttc acttgatcct cgcataccta ctaatagtac cggtagtttt gcttctctaa
     4441 tgcgttcagc taggtaacgg atatcatctg cagaagcaga acctaattta ggatcgctca
     4501 atggcttgat gacattacct tggacaggtg catccactac atcttgcggg atactgataa
     4561 agctcgcgcc tttttttgaa ctcttagcca tacgataagc attggcaatc acttctgaaa
     4621 ttgtttctgg atcttggatt tctgcactgt attttgtgat tggctggaac agtgctgcat
     4681 tatccatgct ttgatgggtc aacttcaata gatcgcttcg ttttacttgt ccggcaattg
     4741 ctaaaactgg gtcaccttct gctgtcgctg ttaccaaacc tgtagctaag ttactagcac
     4801 caggaccact tgttgcgata actactcctg gctctcctgt aatacgtccg attgcttgtg
     4861 ccataaatgc agcattttgt tcgtgacgag tcacgattaa ttcaggacct tgatcctcta
     4921 attcattaaa tacaccatcg atttttgcac ctggtatacc gaatacataa tcaacatgat
     4981 gattaatcaa actttctact acagatttcg aaccttgttt cattttttca gccattgaac
     5041 atcgtccttt ttatgcttta tttttaatta ttagttagaa ataatcacaa ataagtgata
     5101 cagtttctaa acctgtttca tacaaaagta tagcacgttt tatttcattt gtttagccat
     5161 atgtttcaaa aaatgaaaaa gaatgacagt ttttacataa aaaaaactgt cattcttaat
     5221 tgaaagtttc aatttataaa gtctacagat ccatagttct catcaattta cgattgttga
     5281 agtaaatcaa accaaatacg atagaaggac ctagtagaat acaccattgg ggctttacga
     5341 aaaagaagag aaacaaactt gtcaatagta aaaaatctag taaactcaca taccattttt
     5401 tcaacgtata gtagaacgct atccgtagaa cttgatttgg tttcgacgct ggatttctca
     5461 cctgaaaata ccagcagttg gtgctaaagg aaactgataa acaagcaagt aaaacaaaca
     5521 acggaatcaa ccattttcca taagaaaaac gtataaaaaa gaaacaatca aaaatcatta
     5581 tcacactaac tatccaagca aatagccaat aaaaaaatcc ctttagccaa aattggcgaa
     5641 acatctttat gaacaaacgt actggtgaaa aactattttc ttttgctaat tcttctgtca
     5701 tacctatcat cacgatgcaa cctggaccaa taaaaagtaa agaaaaaata aaggaaaaga
     5761 cattccttcc atctatcgac aaaaggaacg cagctgctat atacaagata ttgacacacc
     5821 aaaaacaaag gctacttgcc aatgattgat acaaccaata aaagaattcc atataaacat
     5881 ttttgtcaaa acgctgctct ttcataatca cctcctattc cttcattcct gatgaagcga
     5941 tcccttctac aaaatatttt tgcatcgcta aaaatataaa agcaacagga agaacagaaa
     6001 caactgaggc agccatgatt aatgcgtact ctgcatcaaa tagcccaata aacattttca
     6061 accctaactg gattgtttta ttttccgtag aagacaaata aatgaatggc cccatataat
     6121 cgttccatgt attaacgaat gtgatgatcg tcaaactggc aatcgccggt tttgtaagag
     6181 gtaaaatgat cttgcgatag attccccact ctgataagcc atcgatccgc gcactctcac
     6241 ataaagaatc tgggatacta ctgtaatatt gcttcaacaa gaacacacca aaagcattga
     6301 acgcttgtaa tagcaccaac gaccacaacg tatctgttaa acccactttt ctcatcataa
     6361 tgaactgcgg gatcatataa gactgccaag ggacagcaat cgtgccgata tatgctaaaa
     6421 ataaaatatc gcgcccttta aaatttagtt tagaaaaccc gtaagcagca aaactagaag
     6481 taaacaactg aatcaacgtg atcacaacgc ttaaaaaagc tgtattctta aaaaatgtca
     6541 ctaaaggaat acgagaccaa ataaccagat aattctctaa atgccaagtt ttagggatcc
     6601 attgaatcgg aatcgtgaac acttcattat ttgctttgaa tgaagcagaa accatccata
     6661 aaaaaggaat gatcgtcaat aaagctaata caatcagcag aatcataccc aaccattgaa
     6721 taaagggcat gttttttgaa cgaatttttg ctttttttct ttcatttcga acacctccaa
     6781 agttataatc ttcatttctt tgtaatgatt acaatccaga acaacggtta ttatagctga
     6841 ttcatttccg ttcttcttat ttttgtaagc gcttatttaa caagttatgt aaatctcttt
     6901 atttttttga tttaaaaatg aaaaaataca aaagcaagac tgtcatacgt ctcgcttttg
     6961 tattgaaatt tcttcgatta gagatactta ttactgttca aaagcttctt tgaaacggtg
     7021 aatcaattca ttgactacag gtgataaaat ccgattcttt ttccagacta gcatgcagtt
     7081 cgtttcaatc attgggtcaa gcggaacgaa tgtcactcgc tttgaatgat gacttacagc
     7141 accttctatg actaaagcag cacctacctg attctcaatt agcgcaaata cattgaaaat
     7201 caaattgtac gttcctgtga tcagcaactg ctcctttttt atgcctaacc agtcagcaaa
     7261 cattcgctgt atttctggac gctttgagat caaaagaggt acattcgcaa agtcttcttt
     7321 tgttatcacg tctttttttg ctaaaacaga attttctaaa atgagcaatc cccatttttc
     7381 ctttcttggt aacaaaatcg tttcgtattt ctctgtatct actggatcta gcaaaatcgc
     7441 taaatctaac aggctttttt ctagtcactc cgtgatatca tcacttgttc cgctgtggat
     7501 atgaaattga acgctgggat aatcacggat caacttttct agtaatagtg ccaatgtatt
     7561 cgaactatca gcttccacac agccgatcgt aaatcggccg ctgaataatt gtttttttct
     7621 gttctcgaat tcttgagtgg tttgattcgt taaatctaaa atttcttctg ctcggctttt
     7681 ttagaaaata acctgcttct gtcaagatca ttttgcggtt ttcgcgtaca aacagttctg
     7741 ttcctagtta ctcttctaac tccttcagtt gtcggcttaa ggttggttgt gtaagatgta
     7801 gaacttctgc ggcttgagag attgttcctt cttctgcaat tgtccaaaaa tattttagta
     7861 agcgtaactc catagcgatg ctccttgatt tttttacttg atacattcca ttttatttct
     7921 agataacaca cgatcatatt tcttgatttt tccttgtaaa cgttccaaac attcttgcat
     7981 ttcagcaatt tttttcgtca aaagttcact ttcttcaatc aaaagcttac gccgctcaaa
     8041 tacagttgct tctccttcgc tagataggcg aatatattca atcaaagact cgatcgataa
     8101 tccggcattt cgcatacaga tcgtaaagtc gacccatttt aggtcttctt ctgtataatc
     8161 acggattccg ccttttgtcc gattaactgg tggaatcaac ccgattcgtt cataatagcg
     8221 caatgtatcg gccgaaatgc ccttttcttc actgacttgt ttgatattca tctgatcact
     8281 ctttctctta atttcttgcc actttactta tgtattttat ttattctaca actttaagtg
     8341 tactccaagt caatactcat ttctattaaa actaagctgg attatctata tcggttgttt
     8401 gtgtattatt tcaaataaaa aagtcttgtt tttttattct tttttggata tacttttaac
     8461 aatttaggga aattttagga aaaggggtat aaaaatgaca acgtatcagc gaaaggtatt
     8521 agtatcgaca tcggcgggaa ttgctttaga gaatatggat attatgtttt tagctttttc
     8581 attatcttca atgattactt cttttcatat tagtggtacg caagcgggtt tcatttcaac
     8641 gatcactaat ttggggatgc tcattggagg tatttttttt ggcatcatag ctgataaata
     8701 tggacgtgtg aaagtatttt ctcaaacggt ccttctgttt tcgattgctt ctttattgat
     8761 gtattttgct tcaaacatct acttggttta tctctttcgc tttatcgcag gtgtaggagc
     8821 gggaggagaa tatggggcgt gtatgtcctt gatttccgaa agtttttcaa aaaaacaaat
     8881 tggacgagct tcttcggttg ctggtattgg tgcgcaggtc ggtgcggctt tggctgcgat
     8941 tttagctgct gtgatcattc caacgtttgg ttggaagatg ttgtatgtcg taggtgtact
     9001 gccggtgttg atggtcttgt tcattcgtcg cggcttgact gaaccaaagg aattcaaatc
     9061 tgtacaatct gccgaaaaga aaaccaaact tagcagactg tttgaatcaa aacatcttgc
     9121 ttggcaaaca tgtggcctta gtatcatggt aactgtccaa attgccggct attttgggtt
     9181 aatgaattgg ctaccttcaa ttatgcaaaa tcgattaggg ctttcggttt ctgggtcttc
     9241 attgtggatg gtcagtacta ttttaggcat gtctttgggg atgatgactt ttggcataat
     9301 catggataaa ttggggccta aactagcctt ttctttgttt ttaatttgtt catccttatc
     9361 tgtctatcta cttgtgttag cccatagcca gtggagtttg gtagcagccg ctgtgatagt
     9421 cggctacttt atcaatggca tgtatggtgg atatggcgca gtgattagta gtttgtatcc
     9481 aacagaaatc cgcgcgacgg cgaataattt cgttatgaac cttggacgag ctgttggtgg
     9541 tttttcttct atcgtcatcg gtttcttgat ggatcattat agtttaactg ccgtaatctt
     9601 gtttttgagc ggtatttacc tagtcagtct gctggtcttg acaactctta ctggtgttac
     9661 agcattaagg gaaagcttgc agcattgata gttgagtctg taaatgacaa gaaagaggtg
     9721 tggaaaaagc ttgcatctgc ggtaataaga ccagccaaat caaaaacatg agacactgtt
     9781 tccgtttggc tggtcgtatt acttgatgca gggcagcttt ttcaactgct aatttccttc
     9841 tatctggcgg cgactattct ttgacgtccg tcatttcaaa gtactttact aataacttag
     9901 cataacgaaa aactgtggat acactcgtat attgagcgtc tgcataatct tccaaatttt
     9961 tctcaggatg ttgatacatg aattcaatca aatgataatt atatgatttt ctgtagaaat
    10021 gaagataaat aatgtagatt gggaagttct cctgcttttc catagaaaag cgccgattat
    10081 tctcattctt caaaatgacc ttatcaggat attgttggaa caattcttct aaatctgtta
    10141 gataattcaa gatagactta cttgatacct tcaattcgtt ttgtagttct tctattgaac
    10201 accaacgttc tgtttcaagc agtaaatgaa tcattttaat tttcttttga gaaacttttg
    10261 ttaaaaaagt ctgcatattt tctcctcctg tatctacttt actatactac atacaagaaa
    10321 aaaacggata caaatatcat attgtcacac cgtcaatagt gcataaacct gttattattt
    10381 ctcttttttt cctaagtatt ctattgatat gaatcattta atcgtttatt atataaatgt
    10441 caataatcta ataataaaat aacgtacata ttttattatt gcatacttaa taacagaact
    10501 atagcaaaaa atcccgtgta tcaacacggg attagattaa gtttcctcaa tcactattca
    10561 taacacaatt attataacaa gtaagtaaac aattgaaaag gacatttatt gaaaacggtt
    10621 gttttttact taaggaggaa atttagtgaa aaaattagtt gcgttgtttt tggtcgcagg
    10681 aagtttgttg agtgggtgtg gtgctactac gtctactact caaagcacaa gttcagctca
    10741 ggcttccagc agcagtacca cgatctccga aagtacggca gattcatcga aagaacaaat
    10801 tgaaaagaaa aaagaagaag agaaacaaaa agcggaaaaa gctgcactag aaaaaaaagt
    10861 gacagaagcg gaatctgctt tgtcacaagc tgaagcaaat cctacaaaag aaaactatgc
    10921 aactgcttca gcagctatcc aagcgctacc tgaaaacaaa caagagttga caagccgtct
    10981 tgctgcaatt gattccacaa tcaaagcaaa agaagcagaa gaagaacgga aaaagcaaga
    11041 agaaattgca cgacaacaag cagaacaagc gcaacaagaa gcaatcgcac agcaacaagc
    11101 agaacaggcg aggcaagaag caatcgcaca gcaacaagca gaacaggcgc gacagcaagc
    11161 agcagctgaa caggcttctc agcaggcaca agccgcagct cctgagcaaa ataatgaaca
    11221 aactgtctat gtcacaccga ccggttcaaa ataccataca cataaatgtg gaaatggtac
    11281 gtatacccct gccactttaa gcgaagctca agctcgtggt ttaacccctt gctccaaatg
    11341 ttatggaaac taattcaaaa gttatactat aataataaaa ttcttctgaa acataaaaaa
    11401 atagaagctg gcgttccaga tagtaaacca aaaatagcag tttacacgtc catttcgtca
    11461 gcttctctct tttttcattt agtctgttcc ctctgctttt ttggacatct tcattcattc
    11521 tttctcaatc aaatagtaca cctctttttc ttttcgcagt ttatatcttc cttttttcaa
    11581 tttcattgaa aactgtttcg aatgtttatg ttttacatgg attttaagca ggagctcctc
    11641 atgttgcaag cgatagttcc tgccttttaa atcagaaaat ctcactagtc cgtattcatc
    11701 agaagctttc acaaatggaa ctttttctct agtaatatag tgtttgccgt tgagcgtttt
    11761 tacagaaaag accattcctt cgattggtcg tccgtttgtg ttctttacat aaaaagataa
    11821 aggatagttt cgcttagcga tggccaaaga atctaaataa gcgatgagta gccaaactaa
    11881 gccgattgat ccaagtagta taccgataaa ccaaataaaa aatttccatt ttttccattg
    11941 atcaattcct tggatcgctt tttttgcctc ttttgcttga taaggaatac gatgccccgt
    12001 cacaagcagt cgatgtgtat tgaccatata aggagtgcag gtaagcaaag tcacgagatc
    12061 tctgcctttt tggataccca aagaatctac ttcgtcaggt aagaccactt ggattttttc
    12121 tacttgatac gccaatgtct ttccattgat ctggatgaaa aactggtctc cttttttcaa
    12181 cttaggcaaa tctgtaaata attttgcttc tgggagtcca cgatgcccag aaagaacggc
    12241 atgggtgttt ttcccgccag tcggataaga acttccttcc aataatgacg ttcctttttg
    12301 caaaaatatt tccgttgtct gatcgaatat aggtaagctg acattgattt ttgggatttc
    12361 gatgactcca agcatatgtt ccataaaaaa ggcattacta ggtaaatctt ttaatgcttt
    12421 tgcatcaact gcttggttga aactgcctat ccctggtgat acgttttgct ctgccaactg
    12481 ttggtttttc tttgtcattt tttcctgctg tttttttatt tcctctgaat tcttattcga
    12541 tgcttgcttt tgataatgga tgatcatttg ctgggcaaga aaatcattca gtgaatcttc
    12601 tacaaaagga taagaaaaga tcccgatccc aacgactaaa atgattgcca tgaagatgtc
    12661 cagtattctt attctgcttt tattcatcat gtccacctat tttctaatat gtatatgaag
    12721 aggttgtgaa atcagcgcct tgacctgagt attagcgaag caaacggcaa aatagttcct
    12781 cctattttgc cgtttgttga gcgaatacca agagaccgac cacttgtcac aacctttctc
    12841 cccatttgaa tcactatcgt ttatttatcg tatgattttt gtttccaata gtttaagctt
    12901 ctgtttcttg atggcgtttt ttgaaataga tcactgctcc tccaactaaa gcagtaccta
    12961 ccaagatgaa tacgatgatc cccataccac cagttgaagg caagattcct ttaggttggt
    13021 tatcgatcgt tgtttcatca gaagttgttg gatcatcatc tactgtaaat tcagtgtacg
    13081 cagcttcatt cttaggtaat gcgtagcctt ctggtgcttt tgtttcttga aggcagtatg
    13141 ccccttgttt cagcccttcg attgaaaatt tcccttctga atcagaaatc aatttcactt
    13201 cttcccagtc agaacctgtg acttcatctg accaagcata tgggtattct cctgaggcaa
    13261 ggtttgctat ttttttcatt aatttaacag ttggatcttt tgctcttaga atagaaaatt
    13321 ctgcatcggc taatttttct tgcgtgttca catcttgttt gatgaagtgg aaatcaccgt
    13381 agttttggac attttctaca gtaatcgtgt tatccgtagt tgtattttcc cgattgacta
    13441 caccgtttgg attcccaaga ttggcttcat ctgcgccatg gatgataccg gttgtttgtt
    13501 gcaattttcc gttttttact ataaaagtag tttctttttg tggattggtt aattgataac
    13561 catctggtgc ttttgtttca actaaagtat aattgccgtc tattaatcca ttgacactga
    13621 tggatccttc agtatcagtt gtgaatgtca acgctgactc agcaggatca tagagagcga
    13681 caccagtgtt ttcatcaaaa ccggtcacga attgattctc tgaatttttg atctggaatt
    13741 gcgcccctgc taacttatct acccctgaaa agttcccagc tttctggatt ttgatattgc
    13801 tttctttgat gacattttta gcatataggt gaatcgtgtc taaaggtttg tcagggtcta
    13861 gggtaagctt aacacgatat ttggcgacgt ttcgacaccg ggctttggtg tttctacaaa
    13921 gacgtataca gcatctttcc catcagattt acctggaaga gtcaggttag ctaatccttc
    13981 accagaagtc gttacacttg cgaagggtgt agcgtctgct ggaactgctg tgtctttact
    14041 tggatagtac gtagcagtga tgtcatatac gttgaattca acgccgttca acggttcgct
    14101 attcggaaat gtcaattccg ttccgttgtt tatttgattt tctggtgctg aagtgaattt
    14161 caatttatgc aaattaaccg taacattttc tggtgtaggt gatacggctg cagaaacctc
    14221 tgcagttcct aggccgagaa cgaaaggaag cagcatgata gcagttccta aaaacgtcca
    14281 tatttttgtt ttgatgtttt tcattttatt tccctcctat tgtttccttt ttgttgtttt
    14341 ttataaaacc aagctgtccc tgttgaaagg actagcaata accctaaaat tgagaaacct
    14401 ccaagcatct cttcgcttgt ttttggaaag cttcctccat ttttttcatt tgatggacca
    14461 tttgtctgca tctgaactgg cgtttttcca ttattcgtcg gatttgcttt tggttcgttg
    14521 atgatttcca atggtatagg atgaagcgga tcataactgc ctttttcaac ttcaaagaga
    14581 atggttgatt gactcaatat atatccttta ggcggtttaa tctccactag ttcgtaactt
    14641 ccaaaagaca atccatggac agaaaatgtt ccatctttac tggaagtaaa aatcgttgct
    14701 cttgaatcat ctttggctac ccaatgattt atcccgtttt cttgcgctaa gtattggtca
    14761 ttttttttga ctagaaattg agcatctgct agtttttgtt tcgtttgccg atccactttg
    14821 acaaattgtt tgcctcctgt ttggatggca agatgtgttt ctactgtttg attgtcagtc
    14881 gtcaaacgta catcgttatc aaatgtcgtt ttttcactgt tttctttcaa tgtcatttgg
    14941 taacggatcg ttaatttttt ttccagcaaa cgaactaaga tcttttccag aatctgataa
    15001 gacaaaacca tgttttattt tttgtgtgtg aaatgtatga atcttgtctc caccaatcaa
    15061 aaaatcgatc gtttctggtt ctagccataa ctgaggatca gctgtatctt tgatcgtata
    15121 ggttcctatc tcgttgatgt tctcaggaat tgttacggtg atgagataag ggactttatc
    15181 cccatctgca aaatctgtct gcttattctc tactgttttt gttaatgtga gtgcagcagg
    15241 tattttttgt tcgctctttg gatacaagtg gatggtcgtc aatttatgat tagatgaatc
    15301 tgtcacagga agcacaacga caaatggtgc tgatttttct tttacttaat ctgaagtctt
    15361 tatttccgta aatcggtaaa cagcgtcacg accttgctca tctttgtccg atgcactaaa
    15421 agaagcaatg ccctcttcct cgtctactgt ttttgtcaca ttttctgcca gtattttttc
    15481 actatcactt ttttgtgcta gttttctttg tgcgtcttcc acactacttc cttctgaccg
    15541 cagcttgtaa aattcatccg agacatcata tatagcgaaa gtaacgccgt tcaacccttt
    15601 tactccttga aagttatccg ggatatttat tccgtcgttt tgactgatac ttggcatctt
    15661 atcgctttcg aacattcgat tatgcaaagt aaatgtaata ggtgattctc tagctgacac
    15721 agtcggagaa cctataaaaa agaccagtag gataaaaaag agaacagcaa atatatccac
    15781 acgtacattt tttgtttttt tcatcgctgt tcacctgctt ttttcttacg atggataaaa
    15841 taaaagaagc cagaaaaaat aaagatcagt cctaaaaaga caaagggcag tagacctatc
    15901 ccacctgtcg aaggaagcaa gagagtcgga ttgatcgtca acttagcggt atctgttgcg
    15961 ataatcgtat aattattgtt tacacttcct tcgcctaaac taatcgttac tatttgtgat
    16021 ccaatatctg tccggctgtt aactgttagg gtataatcca atgattcgcc taagacaaca
    16081 ccggtaacgt ggagatttcc aaccgaaatc gagtgacttt gggcatcata attaaaggca
    16141 gcgtcatcga cagtaatcac tgcgttagct ttatagatgg tataagttcc tgatgtgttg
    16201 gctatatcag taaatgacaa cttgggattt gctgctttga ctttgtctat tccgctattc
    16261 gttagtttaa ttgtatagct gccagcatta accatatttt ttgctgattg tgcatctgct
    16321 gtgtaatcca tactgctaag tgccacttgt actggagtaa gcgaactagt gacaccctct
    16381 gctagcttga catggattga cgggacacct gcttttccat catatgtctt actgccactg
    16441 agctctaccg caccgctgat ttgcttaatc gtatagacat cagatgtctg cacgatagta
    16501 aataaatagt ttgttctggc agctagttta gctagtccgc tttttgtcaa ttgccaagac
    16561 gtataggttc caatttcagt aggcgcagtt acttcaactc ccttttcatt catcgctttg
    16621 aaatcagttg gattccatgt gatttgatca atactgagat cattgtttaa aagggatgtt
    16681 gattctgttt tatcattaaa ggcatattta acgatcggta caacagaaag accatcatag
    16741 tacttactgc catttgcatt gattgatact gttgcatttg tcttataata taggatgact
    16801 tcagaaatct gttttccatt tgcgtccttt acgattacat ttccatttgt atctaaacta
    16861 gctaaaacac tcattgctga ttggttcttt gtttctatcg ctgaaacagc tgtaaaggta
    16921 tatccgttga tagtaggcgt attagtcgtc aggtcttgtg cagcacttcc gtaagtaaga
    16981 ttcattattt tagaaggagc aatcatctgt ccaaaatagt cttgatactt aatagtgatt
    17041 ggtgtttgtt tgcctgtata ggtcaccgta tcgacgttac tttctagggc tgtgaaagaa
    17101 ccgtttgttt gtgcaaaaga atagcccgtt actgctgttg gagtgataac cactttgtca
    17161 cccacatgga ttccagataa tgtagtcccg tacgttccga tttttgctgc tccaccattg
    17221 actgtataag aaaaagttcc tgtccgattc gcattgacct gaaccgttgc tgcttgatag
    17281 gttggaaaat ttgaaacatt tcccttacca tcaacgatct gcccattttt gatcgagtac
    17341 cccgtgaatg gaatatcatt tgctaactgt ataccgattg aaaaagtact catacctcct
    17401 ccggcaggaa gccctcccca tgttgagggt gtaaaagaat aacttttatc cgcattgggt
    17461 gtaactgaat cgatcgtatt tccagcggta gccaccccca tatagatact ttttgggaat
    17521 gtgatagtag taccatttac tgtggagcca ataaacagat tatggatatc tggacggtta
    17581 tcaataaaag cgattgcttt ggttacgcct tgtaacgcag ttttatccgc actcatcgat
    17641 ccgttaaccg caacaatgac tccatctttg gcatatatca ctgtatatcc atctggtatg
    17701 gtatgtccgt tgaaattcgc agtggccgca acagtactaa atgtctggct tctacctgtt
    17761 tctgaataag ctttctcccc tgctgctttt gctgctgtga tttctttttc tgtcggatca
    17821 atcagttgag tcgaggatgt ggttgaggtt gtctcgttgt tgctagactg attggtgctt
    17881 tctatggact gtatttcaga atagggtgga ctatttgtct cctctgtcgt ttggttagca
    17941 aaatttgacg tttctgtata aacgtttaca taaatacttg ataagctccc taagaaagta
    18001 aaaatcaaga tgatcgaaat cataatggga tgtaagtatt ttcttttaga tacagctttg
    18061 agtctagaaa caaaatgttt ttgacatata cgttggattt tattttgagt tttcttaatc
    18121 attttcatcc ctcctctcaa taaaaaatca tcaaataatc ctttacacgt tattctaaat
    18181 ttaattttta ttattttatt gatgtataga aagtttgggg ctttttttct ctttttattc
    18241 taaggttatt ttagtcttgt cctttaatac aaagatttta tttttaaata aaaaatactc
    18301 tttttttacg attattgaaa aaaaaagata aaacttgatt aattgaagta ttgcttaaat
    18361 ttggaagcta tgagggaaga ataaaagagt taggacggga ttatctacta tttttctttg
    18421 ttttaaatat ttgttatctt tgaacataaa agagaaatgt gtcgactgca gtgttgtgtc
    18481 cctcatcacg aataaacggt gttcaattag ctgatcttgt attcacttaa aaaatggtaa
    18541 agtacgagtt gctattcccc attttcaatt ttctaacgct caggacaaaa tactgttttc
    18601 acaacctcat tggaataaaa gcatatatat tgtttcaaga acgaaatctt gttatctttt
    18661 taaagggcat ctgtccaaaa tctaatttta ggaggtacgt tttggtattt attcgtacaa
    18721 ataaaagaga attatcttta ttcatgtaac gcatccaatt gattttttca tgtacgctcc
    18781 tggaacgatt ttactgacga ttgcttattt aactgcgaat cgctcattag cttttatcat
    18841 agctatccat attttgaata acgttcttgg ctttgtatta tagatacaga tgacaaaaaa
    18901 agttcaagta tgcggtgtag aaccaatatc gcatacttga acttttttca atgagtataa
    18961 attaaaactt ctaattttca attcccatca tctgatatgc ccgaccgtct gaacttcttt
    19021 tgaaaaagcc aaatgtgatt agataacgtc tttgttcgac ataatcgcca ctagaaaata
    19081 cgttcagcag tatcgaatta acctctgttt ctgtataagt cactccatgg tcaaagcgtt
    19141 tcaagacttc tttcaatagg atgtatttct tttttgttga acttggtact tgttttactt
    19201 ttccatcatt catgaatgtg tctaaaactt tcatcttttc ttgctccgta taagtataaa
    19261 ccatcttctt tctccttttg attctaagaa tactccattt atgttagaat attctggatt
    19321 ttttattaaa gcggttactc ttaagaaaga tattatcata gtgctctttt ttttactact
    19381 acttataggt agatttattc taatccatta ttctccccta ccaataatta aagaaaaaac
    19441 tggatcaggg tgttcaaatt acatgctaag aaaaaagaac ccttgaatac caagggttct
    19501 ttctcagtct gataacaatg attaacgacg gatttctttg atacttgtga aaagtcgtta
    19561 aagtattgat ataaaaggat ttttattacc tctgactaca tattgactaa tttgttaaaa
    19621 aatcgttttt caagaatcct gtaataccag tgctttcacc gtttttttaa ctctctaaag
    19681 aactaaaagc aatcagaggt tttacctttt catttgacgt taagtggcga taaattttcg
    19741 aagttgtttg gtcagtatgg cctaagcgat ctttcaagac ttcccagcta cagtgttccg
    19801 ctactaagct tgccattgta tgacgagcta aatgagtatg aaatggctta ttaattcctg
    19861 agcgattcac gcaccgtttg acgtgatcat ttacatactc tcgacgataa ggaacaccac
    19921 gtttactaga atcaagatac acaaacaaat atgtttcttc tataataaac ctgtaatgtt
    19981 gcctcatctc acccaaagcc ttaatcaact gttgtagctt ttccataact agaggagtta
    20041 ctagaactcg tctagcagaa ctaactgtct tagtagtttc aattcgaaaa tccttactct
    20101 tacctttaac tgccgtttgc ttatagaaca gaatttcatt tcttttgaaa tctatatctc
    20161 caggctgtag agcaagcgcc tcgctgactc ggcagccagt gtaaaataga acatggaaaa
    20221 gtacagacat cggtaattcc tcatgaacaa tgccataatt caaaaaagtt ctcaattcat
    20281 cgacagtaag aaacttgtcc tcaatattat ttatggcttc ttttaaatca gttaccgtta
    20341 actgatattt tggcattctc acagacttca ttggattttc acttatcaaa ttctttcttt
    20401 gacaatattt aaaaatcata tttagaactt gaataagtga ttgtaaacta tttttggcat
    20461 agccaacatt tgttccatta acgccacatt cctttaaggt aaaaagcaat tcttgaaggc
    20521 actctggtgt caaatcagac aactttttat caccaatcca tcgaacaata atttttaatt
    20581 gttcctctcg cttatcatag gttatttcct tttgttcatt taacgaacgg taatattgga
    20641 accattgctc acctacaaca ctaaacttgt cattgcttgc aacaattttt ttcttaagcc
    20701 tttgttgttt ctccatttcc agataagcga gttgcgcctc acgttttgaa ttaaagccac
    20761 gtcttgttgt tcgatatgct tgaaagtacc atgtgatcct accagtagaa ctgatttgct
    20821 tcttgaatcg atctgttttt tccatttatt gattcctcct attttgaagg aacccaacaa
    20881 gcagaaaccc tttggaaatc tttctatcta tgtcatcatt gtaccgcatc ttagaaagaa
    20941 aatgtaactt ataaagatcg aagtagtcgc aatttttctc gatagtcatt cgaatcactc
    21001 tttctcatcc attctaaaac atcatcacga aaatagtgtc ctcgaatccc gtaccaacag
    21061 ggaggaaact caccaagttt tactaaacgg ttcagtgtac tatcacttat ctgaaatagt
    21121 cgctttaaat cagtatttgt taataccaga ggtaaatgac tgactgcaag caatttttcc
    21181 gtgtaatctt ccagtttttt ttgtaaaatt gaatagaact ctatttccag agaattaaca
    21241 tttgttttaa cttccaactc catcaccctt ctctctcaaa atcaaaacga ccaccacggt
    21301 accccttaaa attgtaaaaa aacactccag tactccatca aaacggacta gaaaagagga
    21361 atttttaata tgaaacaagg aattgcagac ataaaaataa ttaaagaaat tcttgaaaaa
    21421 tctacggcta atgcaatagc attcggaaca ggaatcaact tgagtacagt aaaaaaactt
    21481 aaaagtggag agagagctga agaaaaatta aatcttgccg atgctattaa aatcacagaa
    21541 tttggcatga aaaatatgcc aaccaaaatc gaaatatgga aatcctagct cttccaaagc
    21601 catttgtcgt tggtaccaaa cactgccgag acttttccca ataatatcgc caatttcacg
    21661 atcattaaga ccgatgaaga aggacataaa aatgatttct tgtctttgac taggtaatgc
    21721 tgcaatcgca tttgccaact cctcattgga aataaagatt ttcataccaa ggacaataaa
    21781 agtaccacta ctattcaaat ttggttccaa ataatagaac tctgtaccta accctttttg
    21841 ttccaatatt tccattgata tttgccgttg gcgaaatcta gcgtacttct tttgaatatt
    21901 taacgcctca ttgcagacta ctttcttaca aaaactgtca aacgtatgtt gaatgtgggt
    21961 ttcctctgag tcgcccataa tattctcccc ctcaaaaaat tcattcaact cgttttactt
    22021 ctatcccttt ccattattac tacaatctag ga
//
LOCUS       DACDXO010000046        22026 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E23605_2017
            SAMN12639989-rid9881463.denovo.046, whole genome shotgun sequence.
ACCESSION   DACDXO010000046 DACDXO010000000
VERSION     DACDXO010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639989
            Sequence Read Archive: SRR10023389
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22026)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22026)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741914.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:42:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,972
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 33 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 17 of 73
            Pseudo Genes (multiple problems)  :: 10 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22026
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639989-rid9881463.denovo.046"
                     /isolate="E23605_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(61..1371)
                     /locus_tag="IWM35_001903"
     CDS             complement(61..1371)
                     /locus_tag="IWM35_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HAP9919748.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     INFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
     gene            complement(1383..2801)
                     /locus_tag="IWM35_001904"
     CDS             complement(1383..2801)
                     /locus_tag="IWM35_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP9919749.1"
                     /translation="MLSQNAVTANTVKKEKKEFGISDKIGYMFGDLGNCFILGLVNSF
                     LMIYYTNVLGVSGAIVGTLFLISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKY
                     PFCLVTVILFLPFVQHLPMTVKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDR
                     TSLSTFRSIGSAIGGSTTGFFIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYM
                     LTCKLTTERVRVVKNEKVSASQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLF
                     NDYFHNKQAMSVALLFTYGTVVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFL
                     HITNAWVYLILLFIATLGSGLFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARK
                     VGQACAGAVGGFMLALIGYQSSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLV
                     GYPLNRSKTVFIGEELKKINER"
     gene            complement(3041..4609)
                     /locus_tag="IWM35_001905"
     CDS             complement(3041..4609)
                     /locus_tag="IWM35_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HAP9919750.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
     gene            complement(4613..4894)
                     /locus_tag="IWM35_001906"
     CDS             complement(4613..4894)
                     /locus_tag="IWM35_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HAP9919751.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAAGAFSLYRVRMRKGA"
     gene            complement(4891..5814)
                     /locus_tag="IWM35_001907"
     CDS             complement(4891..5814)
                     /locus_tag="IWM35_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectin esterase"
                     /protein_id="HAP9919752.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            complement(5811..6689)
                     /locus_tag="IWM35_001908"
     CDS             complement(5811..6689)
                     /locus_tag="IWM35_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HAP9919753.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPTVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIETLLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            complement(6676..8013)
                     /locus_tag="IWM35_001909"
     CDS             complement(6676..8013)
                     /locus_tag="IWM35_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HAP9919754.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTSAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            complement(8016..9119)
                     /locus_tag="IWM35_001910"
     CDS             complement(8016..9119)
                     /locus_tag="IWM35_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 88"
                     /protein_id="HAP9919755.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            complement(9193..10701)
                     /locus_tag="IWM35_001911"
     CDS             complement(9193..10701)
                     /locus_tag="IWM35_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP9919756.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            complement(10667..11359)
                     /locus_tag="IWM35_001912"
     CDS             complement(10667..11359)
                     /locus_tag="IWM35_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAP9919757.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            complement(11381..12355)
                     /locus_tag="IWM35_001913"
     CDS             complement(11381..12355)
                     /locus_tag="IWM35_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP9919758.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            12655..13527
                     /locus_tag="IWM35_001914"
     CDS             12655..13527
                     /locus_tag="IWM35_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP9919759.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            complement(14109..16307)
                     /locus_tag="IWM35_001915"
     CDS             complement(14109..16307)
                     /locus_tag="IWM35_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9919760.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEVEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLFSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
     gene            16539..17531
                     /locus_tag="IWM35_001916"
     CDS             16539..17531
                     /locus_tag="IWM35_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP9919761.1"
                     /translation="MVTIKDIAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNLAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            17591..19012
                     /locus_tag="IWM35_001917"
     CDS             17591..19012
                     /locus_tag="IWM35_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HAP9919762.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSFPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            19005..20504
                     /locus_tag="IWM35_001918"
     CDS             19005..20504
                     /locus_tag="IWM35_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009224330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HAP9919763.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            20543..21937
                     /gene="uxaC"
                     /locus_tag="IWM35_001919"
     CDS             20543..21937
                     /gene="uxaC"
                     /locus_tag="IWM35_001919"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HAP9919764.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYLTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
BASE COUNT         6669 a         4494 c         3823 g         7040 t
ORIGIN      
        1 atcattaaaa aaaataaagg tcaagctaaa atattattta agcttgacct tattataatt
       61 tcaacgattg tcaaaagtat tgtttgcagc tgtgttgcca gtttctatag ataaatcaca
      121 gcttttgaaa cataagtttt gtactgcttc gattcgcccg aattcttttg cagttatttg
      181 gcaatgagaa aagcagacat cttctatagg cttttcagga aatgctacta aatcaaaagc
      241 tcgggaagga agtgagtaac ctggagaaag gcatgcctgt acgtttttga tgtgtatgtc
      301 tttgactttc gtcatttgct ttgattcagg tatttcttca gccactgctt gccaactttc
      361 tggcatttga gcaaggtcat tgctattttt tctattgtat tcattatgcc agtccattat
      421 ccaagaaaaa ggaaattgta catcaatcat attcagattt tctaccctga tattttcgat
      481 cactccccct ctttcttttg aagatttcat tcgaatacca cagtctgact gaaaaaaatt
      541 gatatcgtga atatatacgt cactaactcc agcactaact tcgctaccaa tagttacacc
      601 gtatcctgaa cggattttac atctagcaac ctcgattctt gatgattttc gattgactct
      661 tgcaccattg ccatctctgc cagacttaat agcaatacaa tcatcaccac aggccaaatc
      721 acattcatag acacgcacat ttgttgaaga atcaatatca atcccatctg tacttggtcc
      781 atcattgttg cggatgatta ctttttctac taatacatcg ttagaataag tgatttgcaa
      841 gttccaaaaa cctgatttct gaaaaatcaa atcacgaaca acaacatgtt ctgcatgata
      901 cagtaaacaa gcgcgtggac gtttaatagc ataatccgca atccaacgaa gtcccttttg
      961 atcgtattca gctcttgtcc cgcttttttg atcttttccc caatacagct cccaccaatg
     1021 gtctccttga ccgtcgatta ttccttttcc ttctataaga atatctttcg ccgagagaac
     1081 attcaaaatc gctgctggcc atttcatttc tacgccggct actcgtgtat cgatttctgg
     1141 aaatgcttcg atatctgttc tccccttgag gactgcacct tcttcaaaga taagatgact
     1201 cttatctttt aaaaaaaggg ctccgactaa ataaatgcct tttggaataa atacaggtaa
     1261 gtgttctttc gcaccttgat cgattgcttt ttgcagtatt attgtatcat cttgttgagt
     1321 gtcgtttgtt ttaaagttca aagcattgat aatccctttt tttgtccgca atacgtttct
     1381 ccctatcttt cattgatttt tttcaattct tcaccaataa atacagtttt tgaacgattc
     1441 agtgggtaac ctactagcaa aatgattgcg gatacaaata agcagaatgc tggtaacaag
     1501 tttgccatcg tataaatcct ttcttgtact aaaactgatt ggatggctcc acctgttgta
     1561 gacgattggt aaccaatgag tgccagcata aatccgccta ccgctcctgc acatgcttgt
     1621 cctacttttc tcgcaaaaga atttactcca tatactgttc catcttctct taagcctgtc
     1681 ttatattgat gataatcaat cacgtcagta atgaatgccc agaccatcaa attgaataaa
     1741 ccagatccta aagtagcgat aaataaaaga atcagatata cccatgcatt tgttatatga
     1801 aggaaataca tcagcaagta catacatgaa gaagcaagta acgcaaatac gcttgcttcc
     1861 tttttaccaa ataattttgt cagctttgta gcaaatggtg ctaaaactac tactgttccg
     1921 taagtaaata aaagagcaac agacattgct tgtttattat ggaagtaatc attgaataaa
     1981 taagtcatat tcgtcccagc taaaatctga ttgatcacaa tgaaaatgtc aacaatgact
     2041 aaaataatca gtgctcgatt agctaacaaa ccttttacca actgagaagc agatactttt
     2101 tcgtttttga caactcttac tctttcagta gtcaatttac aagttaacat atatgcgaca
     2161 aatgctattg cagcacatcc gattgaaatc caaaagaaat gattccctga aacgacttgt
     2221 tgtccactct tgttatgggt atacattaaa atcggaatga agaatcctgt agttgatccg
     2281 ccaatcgcag aaccaatgct tcggaacgtg gacaacgagg tgcggtcatt aggatttgag
     2341 ctaattgctg ctgccattgc tccatacggg atattgattg ttgagagaaa aatcccataa
     2401 acaatgtatg aaacaaaaat ataagcaatc ttcaccgtca ttggaagatg ttggacgaat
     2461 ggcaaaaaca aaatgacagt cactaagcaa aatggatact tcatttttct gatccaagga
     2521 ataaatcggc cagtttctgt taactctgag acatcacata atcttccaac catcacatca
     2581 gcaaacgcat ccaaaattct cgaaatcaag aataacgtac ctacgattgc ccctgaaacg
     2641 ccaagaacat tcgtataata aatcatcaag aaactattga ctaaacctaa aataaaacag
     2701 ttacctagat caccgaacat gtagcctatc ttatcggaaa tcccaaattc ctttttttct
     2761 ttttttactg tatttgctgt aacagcattt tgtgaaagca tttgaattcc tcctacctct
     2821 tctagtaaac gttttcaaaa ttataactct ttttttcttg tttttatatt cacaatgttg
     2881 gctttttatt gcattatctt gacagataaa aaacaaaaaa taatctaaaa acattgatgc
     2941 ataagattga taactttttt tacataacaa tatatacaaa caaaaaaatt cactaactag
     3001 tgaaaataag ttagtgaacc tcatttatta aagaattaaa ttacagatga tcttttccat
     3061 catacttgaa aaactcaaaa tcagcataac ttcctctttt tttatttaga tcatgcactg
     3121 ctatcccgat aaaatttcca gtaaaccctc ctgagagtat acacatgttt ttttgcggac
     3181 ctactttctt ccattcttca gttgatacgt ctttcaaatg aaactgtgct gtctctttct
     3241 ttacagtgac tttcaaaaaa gtcttctttt ctgtgagtgg aatcgtccaa tcttctattt
     3301 gtgctactcc gttttctgat tctatcatac gtaggcattt tcccttgtcc tcattccaag
     3361 taacataaca ataaatataa ttggattcat ttaagtacag taccagtcca gccatctgat
     3421 taaaattatt aggcatgtat tcaaatgctg tctccgcatc aaaagtaaaa tccgtttgcc
     3481 gaatagctaa caaatgatgt tcgaaggtag attgaagaga ttcaccggaa atcagacgta
     3541 aaaaacctgg tctacatttt gtatcacacc aatcagcatt tggcaaaatg cgtctggcat
     3601 tccagttctt ctttagctct ccttcaaatg tatccataaa ctgatgatct ttttcttgtt
     3661 gtacctcttc atttgtctca atcattattt cttccatcgg agttgtctgg ctatctttaa
     3721 gtcttaacca atcgtcggac gtccaataca cttcttgaat agcggtttct cttcccaaaa
     3781 tagccgcacc gtttaatggt ctggtacaaa gatatgtcat gtaccatctt cctgacgggg
     3841 tttgtactaa gctcccatgt cctgtacatt gcaatgtaga agcaggtttg tcactagctg
     3901 tcagcatagg aaaaccagga tcaagctcgt aaggaccagt aatattcttt gctcggcaaa
     3961 ctgtaactga atgtcctctt cctgtaccgc cttctgccgt catcaaataa taatacccgt
     4021 tacgaaaata caaatgtggt gcctccgttt tcgctaattc tgtacctgca aatattttat
     4081 agatgggacc atcaagcttc ttcttctctg catcatattc ttgtaatacg ataccgctgg
     4141 atttattcgg tgtgtccaga cgataatccc atagttcatt gacaagccat ttttttcccg
     4201 tttcaagatc atggaacaaa gaaggatcaa agccgttact atttaaataa attggttcgg
     4261 accaaggacc tgtgatctca gatgcagtga tcaaataatt gtggacatct ttaaacggac
     4321 ggcctgcact tttgacatct gtgtaaacaa gataaaacaa tccatcagca taactgatct
     4381 gtggcgccca tatacttcca tttgtaggat ttccttgcaa agatacttga gtggtcaaaa
     4441 tatccgttgt atgtgaccag ttgactaaat ttttcgactc atatactcgg attcctggaa
     4501 gccattcaaa agaggagaca ataatgtaat accgctcttt cactcttaat atattaggat
     4561 ctggattgaa tcctcttaaa ataggatttt tcactttgat agtctgcatg attcaggctc
     4621 cttttctcat tcttactcga tataaactga atgccccagc tgctaaaaaa gcaagtgaaa
     4681 taaacaacac aacggcaaac agaccagtca tgtcctgtaa taatccaaat gtattttcta
     4741 aagacaaagt tgaatgatct acagcaagcg actgataact ttttttaaac tctgacgcag
     4801 acatattact taaagtaact gctaacccgc caaagaaaat caaagataga ctgccaccga
     4861 ccattcctag caatagttcc cattttcttt tcatttctga accccctgct tttctaaatg
     4921 tatccattca ggtcttttga tcgatgtttc ataattgtta ttacgttcag agaatttgac
     4981 agtctgttca tttttcttgt catcccaatt atcccaacca gttgggtgaa catgcccacc
     5041 tatcttacac gcttcaaata agacatttgc atacggacgc cacggtcttc ctaaatagac
     5101 gtttctagta tcaggttctg cagtcaaggt acagctttca aaacgatacc ctgttttttg
     5161 atttttcggc gtagatgctg ctgtgatgta tccaggattt ttactggcta agctatggat
     5221 atgacactga tgaaaaacgg catctgcccc accaaatatg aaatccaccg ttccttcaat
     5281 ccagcaatat tcataggtct gacgacaata ctcaggattg gcagaagccg gtgtacaaaa
     5341 aggagtacca tctttttgca aatcaggcaa cggacccgtg cataaggtat cctgataacc
     5401 agatagtcga cagtttttta aagatacgcg atctcctgaa ttgaataaag caacagcctg
     5461 tcctaccttt tctcctggac ctgctgtatt ttcgatgtga agattttcta atacgacatt
     5521 cgatccttct aagaaaaaag tagcacttcg gaaagtccct aactggctac cgtcaagata
     5581 ctcctgtttt gcaaaacgat taccggagat aactactgtt cctgctccca caagtttaaa
     5641 attggataat ctgctttcta tatattcatc atatcttcct tcttttacta tcaagacctt
     5701 tggatggcta tccggcaggc ctgccaaata atcgattcct tcttggatag tttcaaaatc
     5761 cgtcggggta tctatagcat tctttccaat tatcagtttg tctttcatcc tcataaatgc
     5821 tcccttatga attgtttcca aatatttctc atttcttgtg tttccatatg accgcctgtt
     5881 aaagaaaaag aagaaaagtt ttctggacta cccattgatt gatacttttt gtccagccct
     5941 tttctcagct tcagtacgcc ttcgtccaag cacatcgtat cgttttttcc aactaagcta
     6001 agacgagggc gcggagcgat tttttcttgg attgctaacg ttgtataagc tgtcaataga
     6061 tttggcacat agtaataaaa cccatgatga tctaggcggc gatgtagcaa aagtgtttcg
     6121 atgtccacct gtgccgctag atcaatacaa actttgacac gagaatccaa tgctgtcacc
     6181 caccagctta ataagcctcc catagaaaga ccaatcgttg cgagccgttg agtatctgtg
     6241 tcttcccttg tctccaaata atcgatcaag ctgatcacat cataaatacg cattccccat
     6301 aacgtttttc ctgttaataa aaattgtttg aacaactcgc tttcagaaat acctccgcgt
     6361 tcttcaaatc cccaagcatc aatcgaccaa acagcatatc ccattcctgt cagtacttct
     6421 gcaaaagatg gagaagctaa gtaaggagca ccatggatca attcactttt cccgactgaa
     6481 aaatctccgc cgtgagaatg caggtaaagg accgtcggaa acggtccttc tttttctgga
     6541 taagcataga gggctggtac tgtttccaat tcatttaagt acaattgata gtattcaagc
     6601 ataaaaccgt tttgtttttc tcggtataat cgttttccac taggggtatg tctgctagat
     6661 ggagcaccta aaaaatcatt caactgcata agcaaagttt ttacgaacaa ccagttcatc
     6721 catattttct gattgatttg attggaattt ctcaatactg atttctttag tattttctaa
     6781 tagaaatgct ggtccctcgt gattttcaat cgacatctgg ttaaaggaaa ccttacccgc
     6841 aaagccaata taaaatcctt gtctgctcat ttcctctatt cctgtcatca tagcaggaac
     6901 accgggaact gcatcttggg ccatcgagac atcgatattg tcaaatgtga tttcttgtat
     6961 gaattgttcc gctaatccat aaataaaacc tgcagaagca tggacattcc ttgccgtgat
     7021 attcgaaaaa tggatccttc tgaatgctga tgtccgttca tctatcggat aagccttttt
     7081 ttcccataca tacggttctt tccctcgcgg accacagaaa taataaagat tcagtataaa
     7141 tggacacatc acattatcca tgactatatt ggatactcga atatcttcaa cgatcccgcc
     7201 tcgacctcgt ctagatttca atcgtattcc tcgatcagtt tcttggaaga tacaatttga
     7261 aatggtaata ttacgaatac taccactcat ttcacttcct aatacgacac cgccatgtcc
     7321 atggaccatc gtgcaatttg tgatcgtgat attttcgcaa gcgatccttt cgtacgtatc
     7381 ttcagtccct gccttgattg caatacagtc atctcccacg tcaatatggc aattactaat
     7441 acgcacattt ttgcatgatt ctggatcaat tccgtctgta tttggtgaat ccgctgggtt
     7501 caagatcgtc aagttatcaa acgttgcatt actgcaaaga atcgggttga tcgtccaact
     7561 tggtgactgg atcaatttaa tatctttcac agtaattcga tgacagttat ggaaactcat
     7621 caatttagga cgaggataag cgagattatc tggttcattt ctaaaagtat gccaccattt
     7681 ttttccattt ccatctaatg taccgaaacc agtgacagag atgttctcta cattttgagc
     7741 atagatacag gaagcataaa cttttcgatg gactccttcc caacgtgaat gaacaacagg
     7801 ataatctttt ggatcgtcag agaatttcag aaccgctcct gcgcttaaat gaagttcgac
     7861 attgcttttt aaaaataaag ctcctgttag aaattcacct gctggaacaa ctacggtccc
     7921 gcctccgtca cttgctgcag catcaatggc ttgttgtatt gcttcagtat tcaattcatc
     7981 aattgatgcg ccaaatgtca aaatatcata catatttata atgcctccac ttcaatcaat
     8041 gcttgcaaga atgcaccaac gcctttttga tcattacaaa taattggttc actgatgtag
     8101 taagcatatg aaccatctcg ctgatctgct ccgcctaacc ctgctacttg acagttttta
     8161 ttcaagttca gccagccttc ttttgtttcc agaacaaatt catcaagtaa tccttgatgt
     8221 gatttcttca cttgtggaag ccattcttct ttgttcagca gtcccaaacg tattccttta
     8281 gccattgcgt aaacaaacat actactgccg gaagcttcca gataatttcc tttccgtttt
     8341 gtttctgttg ttacttgata ccatgtcccg ctgttttctt cttgatatgg aagtaatgca
     8401 gccagtgtct tttctagaat ttctttcagc aaagaacaat ctgtttcttc atgaagtaat
     8461 tcataggtat ctgctgcagc catcaaatac cagccaatcg atcggctcca aaaatgatgg
     8521 gatagacctg tttttggatc agcccatggt tggaccttct tttcatccca tgcatgaatc
     8581 agcaaatttg tttttggatc gaatagatgt ttataactga tttcaaactg tctcaaaaca
     8641 tcttggtaat ctttttcttc actaaaattc tggataaatt cagcataaaa aggagaaccc
     8701 atatataagc catccaacca aatttgataa ggataaattt ctttatgcca aaaagctcct
     8761 tcggatgtgc gtggatggtt tgccaactgc tgggaaagga gttctgctgc ttttttatat
     8821 ttttcttcgc ctgtttcttt gtacaataaa aagaaaagct ttcctgtatt tacatggtcg
     8881 atattgtatt cttcaacgct gtagccacgg atcgttccat cttcttgcac gaaatagtcg
     8941 atattgtctt ttatgaaatc aaaataaatt ttttcttggg ttcgttccca aagtaattgg
     9001 aaccctttaa gtacgacgcc atagtcataa gaccattttc ctttatacca cttttcttca
     9061 tataagcgag gtgttctttc catgatactt tttgctaatt tctttgacca gttttccatt
     9121 atttgctctc ctttaaaaat taaaatagga tttgggacaa ataccaagaa gtatttgtcc
     9181 caatcgtcta tattactgtg ccgctgcttt gtatgcttcc tcgtactctt gtgtgattcg
     9241 atcgcctcct tgtgctttcc agtcttctac agcttttttg aatccatctt cgtcaagctg
     9301 tcccatgatg tattgcaccg tagcatcaat gacgattttt tcaatttctg atcctacttc
     9361 attgttcgtc ggtgaatcca atggcacaga aggatccatt acagcaaatt cttcattttc
     9421 tttgatcaat tgattagaga attctttttc agggtctgta tctttgaaag agaacgtcac
     9481 ttcactagga cgagagcttg ataacggttg cacctcttgt tgccaaagat ccatatcgat
     9541 aaattcgata gaaccatcat cattcaattc ataatgcgtt ccttcgatac cgccagtcat
     9601 caatttatac acatcttctt ctaaaagatc atcaacaaat tgaagtaatt tcttcaattg
     9661 tgcttcgtct tttacttgag atttgggaaa tgctagcaag ccgccaatac catttccttc
     9721 tgaccaaaca tgataaccgc cgccttcacc atctgagatc ttgtttacag gcactaattc
     9781 catatcatct tgaatacctt gacccatcgt cttcagattc ttgatatcca ccattccagt
     9841 gtagattcca gttcttcctt gtgcaaattg ttcttgctga tcagtctttt gggtaactgc
     9901 aaagtcttgt gccaaatagc cattctcaaa tagatctctt gaaaaattca aagctttcat
     9961 atactcttct gtatcaaatt ctggagtgaa tttgccatta tcgtctacag cccagccatt
    10021 tggtgctcca aaataagaag tgaaaagttt aaagctggta taacgaatat cactaggcgc
    10081 acgatcgcca aaaccaactg tgtcgtccac accatttcca tcaggatcgt tttctgtaaa
    10141 ttgtttagct acttcataaa gctcatccat ggtagttgga acatctagtc ctagattatc
    10201 caaccagtct tttcgaatga ctaagccagc acgagcgtag tctttttgta tcggtacgcc
    10261 gtacaaggca ccgtcgatac tcgcagcttc gattctatct tcagaaattt gacttaaatt
    10321 tttatagtct tttaaatatt ctgatacatc ccagaatacg cctgatttta acgcattgcg
    10381 gacagaagag tttttcaaca tcgcaatttg aagtgtaacg atatctccta gttcatcaga
    10441 agccaatgca gttgttaatc tttcttcttt tgaagcatca ggaatccagt tgaatgtcag
    10501 ttccgtatcg gttttttctt cgatcaagtt cacaatcgtg tctgtaggcg gtgaagcatg
    10561 atgcaaaata tttaaccaag tgattttagc ttttccatct tctgtctctg aagaactgtt
    10621 acttgaactg ctgccgcatg cggcaagtgc tagaattcct gcaaaactaa gacctaattt
    10681 tacgagcgtt gcttttttca taaattttct ccttcactgt tttaataata tttttgtttt
    10741 cttctgtttt ttctgtatat gagttcttcc attccaaatg gcaaaatata gaatttgctg
    10801 tccacgtgac tgcggaaaag aagagactgc caccgataaa ggttaaaaca cctggcattg
    10861 tttggatgat catcagataa atccctacca tacttaatat caacactaat gagtaatgaa
    10921 gatgtgagaa tccgaataaa aatgccattt tcattttgaa aaaatatcct ttccaatcat
    10981 aatgagccat gattgggaaa atatagagtg ctgagagcac atatatgaac gcagcaatat
    11041 acaaagcgat cctaagatac caattgtgta tgaaagaaag atccacaagt aaaacaatcc
    11101 cgatggcgcc atatatccag ctcatcagca aggtttcacg atagttttct ttaaaatatg
    11161 tgaagtacgt tttcgtcaaa ggaaacgttt cttttgagcg cagccatttt ctaatcactg
    11221 caaccatcgc atacgatgca ggaccgaaag taaaaactcc caaacctgcg atgactaaac
    11281 cgccccacag aaaattcaaa taaaccaact gtaaaatacg atttagcctt ccgtttattt
    11341 tatctaatga ctgcagcata tctttccctc tctttctatc ttagtaaacg ccgtcttctc
    11401 ctagcttttt ggcaatctta ttagctaaaa ttaccataaa caatcctacg acccctttaa
    11461 acaatcccat tgctgtactg aaacttaact gtccattctt caatccagct gtataaatat
    11521 aggtgtcgaa aatttctgca acgcttcgat tcaatgaatt caataacaag tacatatgct
    11581 cgaaccctaa gtctaatgtc tgaccgattt ttagaatcag caaggtaatg attactggac
    11641 gaattgccgg caatgtaaca tgccatgttt tgcgtaagcg tcctgctcca tccatttctg
    11701 cagcttcata caactgtgta tcgactgcag ttatcgctga aaggtagatg atcgtagacc
    11761 agcccaactc tttccagatc acttgtccga tatatactgt gcgtgtccat tcaggggatg
    11821 ttagaaaact gattttttca aatcccattg ttgcaaggaa ttcattcaat acccctccgt
    11881 caacattcaa aaatacataa gtaatcgaaa caatgattac ccaagacatg aagtgaggga
    11941 tgtaaatgat cgtttgaatc ccgtttttca atcgtttgtt cgttacttca tttaatagta
    12001 aagacaaaat gattggcatc ggaaaaaaca cgaagatatt taatccgaac aagatcaacg
    12061 tatttcttaa taatgtaaag aatgtcggtt ctgtaaacaa acgaatgaaa tgtttgaaac
    12121 cgacccaagg acttcccatt attcctaaaa atggttgata atcttgaaaa gcaataacga
    12181 gtccccccat aggaagatac ctaaaaatca agaaataaag gactcccggc agaatcatca
    12241 catataaaaa tttattcgtt ttgattcctg caattatttt ttttcgtctt ctgagctttt
    12301 cttctttttt cgatagcgtc agttgaaatg gttcttgttt ctttacactt tccataagcc
    12361 cctccttagt tttgtgaatg tttgctgctt atgacttcat gatagataac tatctgaatt
    12421 acgtatataa tcatttttat cgtaagtaaa aacgcttaca tagcaatgat ttttgtgcta
    12481 tacaaaacga taaagtgtat tttttcacaa ataaactacg catagcaaac cttctatttt
    12541 tttctatctg acacttagca taggaaatga ttatatacgt aatctttcac cgatgataaa
    12601 ttgaacatat agaaacagaa acatttaatt tattttgagg aggagcctat ctatatgaaa
    12661 aagaaagtct ctataggaga aagaagtttt acacttttca acaatacgtt tctaattttg
    12721 ctagctttga tctgtatcgt tcctttttta aatatcatcg ctacatcttt tgcttctaca
    12781 caagaagtcg tcgcgaaaaa gtttatcctt tttcctacta ccttttcttt agacgcgtat
    12841 cgctacattc tttctacgcc gaccattttt agagcacttg ctgtttcgat tggtgttaca
    12901 ggtgtcggaa cgatcgtcag tatgtgtgca acttcactga tggcctacgg tctttccaga
    12961 aagtatttgt ttggtcgagg tttcgtcaac tttcttgttg tattttctat gttgttcagc
    13021 ggggggatga ttccaacgtt cttagttgtt cgttctctag ggctggtcaa ttcttactgg
    13081 gccatgatcc tgcctgtggc tgttaatgca atgaatatga tcattatgag aaactttttc
    13141 caagccttgc cggatagttt ggaagaatca gcaaaaatgg atggttgcac agattttggt
    13201 gtatttttca aaattatgct gccattagct ttaccgtcta ttgctactat ctcgcttttt
    13261 tatgctgtta cttactggaa cacttatatg actgctattt tgtatatcaa cgattcatct
    13321 aaatggccga tacagattct tttacgccaa atcgtcattg tatcaagtgg gatgcaagca
    13381 gaaagtagtg ccgttgatgt gattccacca gcacaaacag ttaaaatggc tgttatcgtt
    13441 attgcaacag tgcctatgct gattgtttat ccttttgttc agaaatattt tgtcaaagga
    13501 gcactcgtag gttctgtcaa agggtgatta tgtactgaac gcaaaatggt agttgtgtca
    13561 gaagtggtca gcttcaagaa ataagaaggc atttacgaaa attgcttctt aaatttttgt
    13621 gaaatttcgg cttatttttc gaagaatcta ctcctggaac accgtgtatc agaggttgtg
    13681 aaagagacgt tcagccttga gcgatgataa aaactctcac aaacattgcg acgagtaacc
    13741 cacaggagca ataagaagcg attttgaaaa atagcttccc atatttcaca aaattatgcc
    13801 ttattgtcga tagcctgtct cttgaacgcc atttattcgt aaaaaagaac tgtgacaaga
    13861 cttttgtcac agttcttttt gcgtgcttag ccttgaaatt taactatcca taaatacaaa
    13921 tactagggtt attctaactt cttttattgc ttgaatcagt ccacaagaaa aacagcctct
    13981 tgattaaacg gcgtttatct tgttgattct gcggcaataa aatcagccag cagcaaaaca
    14041 tgaagtgctg ttttttgctg tcctctttta ttgcttgaat cagatcacaa gaaaaacagc
    14101 ctcttgattc acaaatattt ttttcgatat tctcccggcg taatcgattc cttcttttta
    14161 aagaaacgaa taaaattttg cggatttcga tattgcaatc tttctgcaat ctcttttacg
    14221 gagaggttag tctctttcaa ccattttttt gctacctcta aacgatgatt ttgaacaaaa
    14281 tcaccaaatt tttctcctgt ttccttttta aatacattac ttaaatagtt agggttataa
    14341 tgcaacctat cagcgatcac atctaaagac agatcacgat catattctgt gtagacgata
    14401 tgcataatat tctccgacaa atttttaaat tcatgatcag tgattgcttg agagcattct
    14461 gtgattggca gaactaattg ttctaaaaca agtttttcta gttctttggg atgataattg
    14521 ttcagtgctt ttacataaat ttgtttcatg ttatcaaagt tttttgaatc tactcccaat
    14581 aactgtccta attgaacgag ttcattgatc aagcggaccg aaactacttc ccgactcaaa
    14641 ggatttttat tttgtgcaaa caatgcatca atcaattcat gtactaaatt cttcacttcc
    14701 tgatttccag aacgaatggc ttcaaacaat cgattttgtt cttctatcgg atatctaata
    14761 agtgtttttt catgagcagc aggaacgatt tcctgataaa aaataattga acttggacca
    14821 gtatttacac gataatacag ggcttctttt gcccgatcaa ccgattgctt gctttcattc
    14881 agcgtttcga accggttact gatgcctata ctgatagtta aattcaagta ttcttttaca
    14941 cgtttttgaa tgagtcggca atactccatg acttttttgc ttgcttctgc atcatcttta
    15001 tccattcgat aaatagtggc ttgcatttct tcattcaaaa cgatcggcag cattcgattt
    15061 tcctttggca caatttccgt gatcatgttg ttgatcgcta aaagtagcaa atcacgctcc
    15121 ccaccgtgac tatcttctaa cagatcgatt tgaaccaaag ctgtataata gcagtcattt
    15181 tgagaactat aaccaaattg ctgcaaccgc tgatctagtt ctcgcctttc tactcgattt
    15241 ctaaataaac taagtacaaa tagcgtttcc agctgtggct tttgggtaac taagtgtgca
    15301 cttaacgctt ctttttctcc tatgatccga ctgactgatt gagcgaccaa atctagttct
    15361 ttcccactga atccttttga tttaagattc agacgttcct gtatttgaga aatcggacgg
    15421 gcaaatctgt ccgaaaataa gtaggaaaaa agaccaatga gtcctaccag tgttaaagac
    15481 actgcaacta gcccgattct cagattgcgg atcattgcgc caattgcact agagtcaact
    15541 tctaatctgt aaatccaatt attataatct gatttcagta ctatatacgt tttatctttt
    15601 tctcttatca ctttcagctg attgtaatct agatccacgt ccgttaatac agttggttgc
    15661 tctttatttt ttactgtctg agtagtaaaa aggttgccaa cttcgttttc aatagaaagt
    15721 atgtcttctc cactttcacc aacaacttgt tctattgtat gtttctctat cgtagcaatt
    15781 cccaatgcat atttttcttt ttcaaacatt ggcaaagaaa tcaccatttc gattcctttg
    15841 tttttctttt gccaaaataa attatttttg ttatttatgt acttctgttg atacttttca
    15901 acttcctctt cggtcagttg actcaacgat ccattgatta ctccccattt accagccaaa
    15961 ctgactaaat cataattatt atcatccata acaatcgttt ctataaaatt tagttctttc
    16021 ctgatttcag aataagtgga gtaatctgta tatgtcagcg gatgcttgaa tatgtccgag
    16081 aaatttttgg tcgaaccata cgtagaaaac gaatattcaa tcgttctaat tttccattca
    16141 acgttagttt taacttgcct gagcaaattc tttttgtctg ttaaataact cgattcgatc
    16201 gattcatagg tattctggta aatgaataaa ctaaatccta tgacaagtac ggctcctaat
    16261 aaaaacatcg gtaaaaatat tttataaaat actgtacttt tcttcatttt cttcctctcc
    16321 tccttttaaa ataaaaggct ttcatttttc tgtaaaattg atactcatct aattaaatga
    16381 gcattccccg ctttcaagaa tagcataatt aaaaataaaa atccaagtaa tttttaacgt
    16441 gtgcaatctt ttcatatcaa agatctattg acttatcgta gttatcgtaa tatgatgaat
    16501 gcgttaacaa aaagtgaaaa agtaaggaaa atgatggaat ggtaacgatc aaagatatcg
    16561 ctaaagcagc tggcgtttct catacgacag tttctcgtgc tttgaataac agcagtttaa
    16621 taaaaaatga aacaaaacaa aaaatacgac aactagctaa ggaaatgaat tattcgccaa
    16681 actatagtgc aaaaggttta gtgaatcaaa aaaaatatct aatcggacta tttttctcaa
    16741 gtttgcataa aggaacttca tcaaatttcc tagcagattc cattgcaggc atccattcag
    16801 tattagacac aaattacagt ttatctgttg aaagtatcga tgttgttgat ttaacagaaa
    16861 tcaacctgca acgctacgat ggactgatcg ttatgagtca aagtgatgat gatcagcctt
    16921 tcatcgacta tctcaaagaa aaagaatttc ctttcgttgt tgtcaatcgt cacatcaatg
    16981 atccagcggt tatcaacgtc actgccgatg atgcggccgg tgttatgcag gcagtagatt
    17041 acggtattgg atgcggacat acgaagatcg cttatattgg cggaaagaaa aattttcgtt
    17101 cgacaaaaga acgggaaaaa ggggtattga aaagtctaaa aaaagcgagc atccctgtca
    17161 atctagccta ttttttaaca ggagattaca gcttagaaag cggcctccaa aacatggaat
    17221 acctattatc actttctgac ctgcctactc tggttttttg cggcaacgac gacatagcga
    17281 ttggtgcatt gagagctgta catttagctg gcctccgtgt tccccagaac atttctctga
    17341 tcgggttcga tgattctcca gtcgtctcct atctcaatcc gccgcttacc accgtacgta
    17401 agcctttgaa agaaatgtgt caagaaggaa caaagctttt actagatttg atcgaaggca
    17461 ggacgatcga acaatctaaa attgagctct ccaccttgtt gatccttcgt gagtcgatca
    17521 attacttgta aactacaagt aattttatat aaaaatgtta acgtgtgcat aacacagaaa
    17581 ggaagtttat atgaaaaaac tgacaaaaga attagtgaaa aaaaacgaag cacctgtaaa
    17641 agttcttcag tttggtgaag gcaattttat gcgtggattt attgattggc agattcagca
    17701 gctgaataac caacaattgt ttaaaggtaa tgttgcaatc attcaacctt tagaacaagg
    17761 tcttggaaat atgatgaacc agcaagataa tttatatacg gtcattttac aaggactcag
    17821 caatggcgaa atcgttgata cttccgagat tatcaccgta gtagaacaaa tgatcaatcc
    17881 ttatgatgaa tgggatgctt atctatctct agcagaaaat gacgacttgg cctatatcgt
    17941 ctccaataca acagaggcag gaatccaatt cgtggagact gataatttaa cagatactcc
    18001 cccacagagc ttcccgggca aactgacagc ctttttatac cgacgtttca aattaaacaa
    18061 agctggcttt accatcattc cttgcgaact gattgacaga aacggcgaac gtctaaaaga
    18121 aatcatttta cgctacgcag aaaaatggga tttagaacct gcttttgttg agtggttaga
    18181 aaaagaaaat gttttctgtt gtagtttagt tgatcgcatc gtcccaggtt atcctcgcga
    18241 agatgcaggt gttttcaatc aacggcataa ctatgaagat caattgatgg tcaaagcaga
    18301 accattcatg ctttgggtga tcgaagggcc taaagaattg gagaatcaac taccattcaa
    18361 acaggctggc ttgaatgtca tcatcacaga tgatatgact ccttatcgtg aacgtaaggt
    18421 gcatttgctt aatggaccgc atacagcgat ggtccctctt gctttgttgg cggaattgga
    18481 aactgtagaa gaagtaatga aagatccttt attctccgca tatgttgatc agttattcaa
    18541 tctagaattg attcctatgc tttctttacc taaagatgaa ttagccattt atgctgacca
    18601 aatcaaagaa cgtttcttga atccttttgc ccatcacaaa ttagaagcga tttcattaaa
    18661 tagtgtttca aagttcagca cccgtctgct tccagtgttc aagaagtata tcgaagaaca
    18721 aaatcaggta ccaccgttaa tcactgtttc actagctgcg ctacttctta tgtatcgagg
    18781 cgatcaagta aagccacatg atgatgaaaa gacgataagc gaatttactg atgcttggtc
    18841 tgataatgga acagctattc ctcgattatt gcaaaacgct gcgctttggg gagaagattt
    18901 gtctcaaata ccgaatgtga cagatacagt tcaagaaata gctgcaaaga tcgatcaaaa
    18961 aggtgtccgg tcagtattac aagaaatgaa aggaacgatg gtccatgctt gaaaccctgc
    19021 aaccaacaat gaaattaaat cgacgagaca gtgtggtcgt cgcattaacc cctattccaa
    19081 aacaaacaca actgacgatt gatcagcaaa cgatcaccac tcttgaagat atcccgcaag
    19141 gtcataagat tgcattgggt gatttgaaag ctggagacaa cgtgatcaaa tatggctatc
    19201 caatcggtca tgtgacgaca gacgtaactg ccggtcaatg gctgcacact cataatgtaa
    19261 aaaccaatct ttctggtgaa ttggattatc gatatgaaaa agatgtccat ccaagtcatt
    19321 acctttttga gaaccgaaca tttcaaggtt acttgcgaaa aaatggaaaa gtagggattc
    19381 gtaatgattt gtttttagta cctaccgttg ggtgtgtgaa tggcattgct gaactgatcg
    19441 tcaaacagtt caaggaaaaa catccagatc tgggacagtt tgaccatatt acgattttaa
    19501 agcatcctta tggctgttcg cagcttggaa aagatcatca aaatactcga gaaattttag
    19561 cggacgcagt aaatcatccc aatgccggag gagttttagt atttggatta ggttgtgaga
    19621 acaatacagt tcctgaattc aaaaaaattt taggtgaata tgatgaagaa cgagtgaaat
    19681 ttctagtggc tcaagatgtt tacgatgaga tcgaacaagg tgtggctcta ttagaagagc
    19741 ttttaacagc ggcggaaaat gaccagcgca tatcagtccc gctctctaag ttgaatgtcg
    19801 gtttgaaatg cggcggttct gatggtctat cgggaataac tgctaatcct ttacttggtg
    19861 cattttctga ctacttgatt gctcaaggag gcagcacgat cttaacagaa gtgccagaaa
    19921 tgtttggcgc agaacaagta ttgatggcac gagcggaaaa tgaagaagtt tttgactcaa
    19981 tcgttgactt gatcaatgac ttcaaacagt atttcttgtc ttacggggaa ccagtttacg
    20041 aaaacccatc tcctggtaat aaagcaggtg ggatcactac attagaagat aagtctttag
    20101 gctgcacgca aaaatcagga agctctcctg tcgttgatgt gctacaatat ggcgaaaaaa
    20161 tcagaaaacc agggcttagt ttattgcaag ctcccgggaa tgatcttgta gctgcctctg
    20221 ctcttgcttc atccgattgc cagttggtct tgtttaccac aggaagaggg actccgtttg
    20281 gctcatatgt cccaacggtc aaggtatcta cgaatacaac tctttttgaa cgcaaaggtc
    20341 attggatgga ttttaatgca ggcgtcttat tagaacaacc aatggaagta atcttagaat
    20401 cgtttgttca aaaaatcatc gctgtagcta gtggagaaga aacgaaaaat gaacaaaatg
    20461 acgttcgaga aattgccatt tttaaaaacg gagtaacact ttgattttca taaaacagta
    20521 taaaaagaaa gaaggaagat ttatgttttt atctgatgat tttttattaa aagacgattg
    20581 ggcgaaaaag ctttaccatt cctacgcaaa aaacatgccc attattgatt atcactgtca
    20641 cttagatcca aaagaaattt acgaaaacaa caatttcagc aatctgacag aagcttggtt
    20701 aggaggcgat cattacaaat ggcgcttgat gcgagcttgc ggcgtgcctg aagaaaaaat
    20761 cacaggaaat gcttctgatt ttgaaaaatt tttagcttgg tgtcaaaccg taccaaaaat
    20821 tatcggaaat ccgctttaca gttggtcaca tcttgaattg aagcgtttct tcggtattga
    20881 cttgttgata aatgaagaaa atgcagagaa aatttggaca caagcaaatg aaattttggc
    20941 agctcctgat tttcgccgtc gggaaatcgc aaaaaaatcg aatgtagaag tgatttgtac
    21001 aacagatgat cccgcagacg acttgcacta tcatcagttg cttgctcatg aagaaccaga
    21061 tttgaaggtt ctaccttcat tccgtccaga taaagcactc aatattgaaa aagaaggatt
    21121 taatgcatgg gtcacactac tggaagaagc agctgaccaa acgatcacca cctatcaaga
    21181 actgatagaa gtcttagggc agcggataga atacttccat caacatggat gtcgtgtttc
    21241 cgaccacgct ttagacatct tacgctataa agaagcggat gaaacagtat tagaagagat
    21301 ttttcaaaaa gcacgagcta acaaactatt atcttcagat gaaatcgatg cttatcttac
    21361 agaaacactg atacatttga cccacttcta tcattctcat aattggacga tgcagctgca
    21421 catccatgct tatcgtaact gtaatacaca aatgttggaa aaattagggc ccgataccgg
    21481 ctatgatgga atgaatgatc tttctttgac tattcctctg caaaaattat tgaaccgtgc
    21541 agaagaaacg gatcagttgc caaagacgat cctttattca ttgaatccga atgactatcc
    21601 tgcgatttta gctttgatgg gctgtttcca aaaagaacag aatggaaaac tgcagttagg
    21661 ttctggatgg tggtacaacg atactcgagc tggtatgcgc gatcaattga cacaatttgc
    21721 tgatggtagt gctttgggga attttgtcgg catgctgaca gattcaagaa gtttcctgtc
    21781 ctacactaga catgaatatt tccgtcgtgt tctttgtgaa tttttaggcg aaatggtcat
    21841 gcgaggagaa gcacctgaag atgaacaact tttaggaagt ctagttcaag cgattagtta
    21901 tacaaatgct aaaaattact ttggcttttt tgcctaattg attaatagta acagtaacga
    21961 atcaaaaaca ggatagtgaa ataacggaat ccgtttatcc acgttttaga gaagaagacg
    22021 gaccaa
//
LOCUS       DACDXO010000047        21911 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E23605_2017
            SAMN12639989-rid9881463.denovo.047, whole genome shotgun sequence.
ACCESSION   DACDXO010000047 DACDXO010000000
VERSION     DACDXO010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639989
            Sequence Read Archive: SRR10023389
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21911)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21911)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741914.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:42:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,972
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 33 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 17 of 73
            Pseudo Genes (multiple problems)  :: 10 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21911
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639989-rid9881463.denovo.047"
                     /isolate="E23605_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(459..1088)
                     /gene="udk"
                     /locus_tag="IWM35_001920"
     CDS             complement(459..1088)
                     /gene="udk"
                     /locus_tag="IWM35_001920"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HAP9919765.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
     gene            complement(1172..1867)
                     /locus_tag="IWM35_001921"
     CDS             complement(1172..1867)
                     /locus_tag="IWM35_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="noncanonical pyrimidine nucleotidase, YjjG
                     family"
                     /protein_id="HAP9919766.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            complement(1880..2596)
                     /locus_tag="IWM35_001922"
     CDS             complement(1880..2596)
                     /locus_tag="IWM35_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HAP9919767.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            2926..3534
                     /locus_tag="IWM35_001923"
     CDS             2926..3534
                     /locus_tag="IWM35_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HAP9919768.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEEESTGEGGEPEVIGSTEE"
     gene            3859..4182
                     /locus_tag="IWM35_001924"
     CDS             3859..4182
                     /locus_tag="IWM35_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HAP9919769.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            4364..5734
                     /locus_tag="IWM35_001925"
     CDS             4364..5734
                     /locus_tag="IWM35_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HAP9919770.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(5864..7165)
                     /locus_tag="IWM35_001926"
     CDS             complement(5864..7165)
                     /locus_tag="IWM35_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HAP9919771.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYYYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKTE"
     gene            complement(7519..9510)
                     /locus_tag="IWM35_001927"
     CDS             complement(7519..9510)
                     /locus_tag="IWM35_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter Kup"
                     /protein_id="HAP9919772.1"
                     /translation="MATTHNTKKISMTGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGL
                     SGINPDFVIGSVSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLII
                     PAMIGGAALLADGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFM
                     VQRFGTDIVGKAFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKL
                     GLFILGNIFLATTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQN
                     PEAQQIKNLNPFFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPR
                     LKIMYPGNSIGQMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALL
                     YFYLIQNGHSRWLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWE
                     QGNIIRESSTEDVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSI
                     LDKRPKRARVYWFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQ
                     VIHDLMKQGRLPKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAI
                     KKIATSPETWFGLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            complement(9654..9920)
                     /locus_tag="IWM35_001928"
     CDS             complement(9654..9920)
                     /locus_tag="IWM35_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAP9919773.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            complement(10430..12556)
                     /gene="pnp"
                     /locus_tag="IWM35_001929"
     CDS             complement(10430..12556)
                     /gene="pnp"
                     /locus_tag="IWM35_001929"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HAP9919774.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            complement(12744..13013)
                     /gene="rpsO"
                     /locus_tag="IWM35_001930"
     CDS             complement(12744..13013)
                     /gene="rpsO"
                     /locus_tag="IWM35_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HAP9919775.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            13264..13827
                     /locus_tag="IWM35_001931"
     CDS             13264..13827
                     /locus_tag="IWM35_001931"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HAP9919776.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            complement(14080..14601)
                     /locus_tag="IWM35_001932"
     CDS             complement(14080..14601)
                     /locus_tag="IWM35_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HAP9919777.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(14604..15446)
                     /locus_tag="IWM35_001933"
     CDS             complement(14604..15446)
                     /locus_tag="IWM35_001933"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HAP9919778.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELVALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            complement(15439..16164)
                     /locus_tag="IWM35_001934"
     CDS             complement(15439..16164)
                     /locus_tag="IWM35_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HAP9919779.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEIAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            16340..16636
                     /locus_tag="IWM35_001935"
     CDS             16340..16636
                     /locus_tag="IWM35_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9919780.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     gene            17204..18796
                     /locus_tag="IWM35_001936"
     CDS             17204..18796
                     /locus_tag="IWM35_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750719.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP9919781.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            18809..19549
                     /locus_tag="IWM35_001937"
     CDS             18809..19549
                     /locus_tag="IWM35_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAP9919782.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
     gene            19717..21066
                     /locus_tag="IWM35_001938"
     CDS             19717..21066
                     /locus_tag="IWM35_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP9919783.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(21194..21784)
                     /locus_tag="IWM35_001939"
     CDS             complement(21194..21784)
                     /locus_tag="IWM35_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HAP9919784.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVIVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
BASE COUNT         6294 a         4462 c         4158 g         6997 t
ORIGIN      
        1 gggggtgcga caaaagagct tcttcaaaaa aataagtcga aatctcacac aaattcgaga
       61 aataattttc gtaagtttct tcttatttat tgcatctgac cacttctgtt acaacctctc
      121 gttaaacggt gttcaaaagc ttgcatctac gataataagc ccaacccaac gaaaaacgtg
      181 aggagctgtt ttatttggat gctcttatcg cttgatgcaa agacagcttt tttacaacct
      241 ctacttcacg gtgctcaatc agctgacctt cggtattagc tcaaaaaacg gtaaaatata
      301 aacagctatt ttccgtttct tctttttaat acttaggtca agatgctgtt ttcacaacct
      361 cttgttataa ataaaaaaac agttggctgg tgactgttca aaaagcagaa tccttgcttt
      421 tcaaacggtc gcagtccgtt caactgtttc gctgcgcact atttatggtt taagaaacta
      481 gctacttttg ttgtaatcaa atcaatagca acgtggtttt ctccgccttc tggaacaatg
      541 atatccgcat agcgcttcgt tggttcgata aattgatgat acatcggttt aacaactgtc
      601 aaatattgtt cgatcacgga gtccaatgtg cgtccccgtt cttccatatc ccgtttgata
      661 cgacgaatga tacgaatgtc atcatctgta tctacgtata ctttgatatc catcaagtca
      721 cgcagacgtt cgtcttctaa aatcaggatt ccttctagaa tgatcacttc ttttggttcc
      781 tgaatgatcg ttgcctgact tcttgtatgc gctacatagt catacacggg tttttcaatg
      841 gctttatagt tcagtaagtc gcctacatgc tgaatcagca gatcattatc aaaggcaaat
      901 gggtgatcgt aatttgtgtt caatcgttct tcaaaactca aatggctctg atccttgtaa
      961 taagaatctt gttcaagcat catgatggaa tggtccggga agttgttgaa aatcgcacgg
     1021 ctgacacttg tctttccgct tccggatcca cccgtcacac caatgataat tggcttgctt
     1081 tttgtcattg actaaaacct ctttcgtatc cgttttttca ttctaatcta ttatagcgaa
     1141 aaaacacgaa aatgctatat ttttctgaaa attaatcctt ttcaatttcc aataatgaat
     1201 aaagttcctc taatgaatcg attcgataag taggctggat ttcttcgatt tctggtaatt
     1261 tatttctatt gaaccaaatc gtatcgatcc ctgccatatt tcctcctttg atatcagaag
     1321 tcaaagaatc accaatgatc acagtttgct ttttatcaaa attaggaatg tgggaaaaga
     1381 catgatcaaa aaattctttc atcggctttt gcgctccgac agcttctgag acaaagatat
     1441 ccttgaagta atcaagcatt cctgcatctg ttaatcgccg atgttgcgtg tgcgacacac
     1501 cgttcgtcac aatataaaga tcagcatgcg gatggacctt tttcaggatt tctagacttc
     1561 ctggaacaag atcatgacct tggttcaagt agtggcggta gcgtttttcc atttgatcac
     1621 tatctactgt tttcccaaat tgctggaaaa gtaacccaaa acgactgcct attatttctt
     1681 ctcgtgatag tctgcctttt tcaaactcct gccataaatg atgattcaaa gccttatagc
     1741 tggtttcgat ttctggtgtc aatgtgattc cttgttctgc aaacaaagca cttaaagcat
     1801 tggcttcagt taattgaaaa tctaacaatg tatcatctac atcaaacaat aaagttttat
     1861 atttcatctt tttgacctcc tatttaaaat aaatacttaa ctttttcatc tctgctaatg
     1921 tcaacgatcg atactcgcct actcctagct gttcatctaa ttccaatggt cccatgctta
     1981 gccgtttcaa tgcggtcact ttctttcctt gggctaagaa catctttttg acttgatgga
     2041 attttccttc ttggatcgtc aataaaaccg tactttggtg cttgtctgct ttgagtattt
     2101 ttaaatcagc cggcttacat tgatacccat cagcgaatac gattccattt tcaaatgctg
     2161 ttacgtctgc cggtgtcacc ctttcgttca caactacttg ataagtcttt gaaacctttt
     2221 tttctggaag tagcatttca tatcccaatt gtccattcga agtgatcagc aatagtcctg
     2281 ttgtatctct atctagtctt cccactggat ataatggttg gaaacgatcg gcacttgcaa
     2341 tcagatcgat tactgtcgtc ttttctggat ctgatacagc tgtaacagct ttttcaggtt
     2401 tattgagaag ataataggac tcatttgttt ctaagtgttc acctgctact tcgatttgat
     2461 gcagtaaact atctacattt cgattttgtc gaaattcgac tgtgccatct atcatcactt
     2521 gcttcaaagc aaacagccgc ttcatttcct tgcgtgtcgt ttctaatttt tcttctatta
     2581 atttatctaa acgcatgctg tttcctttct tattactact aattaattat accttgaata
     2641 ttcttgcttg caaatacatc atcatagtta ttccttgact taattaacag ttcacgttac
     2701 ggttaaatag taaagtgaat tggatttctc atacacagcg taatgacctg gtggagaaaa
     2761 gttgaatcct gactttctaa ggggagatgc gcgcatctct ccttttgcgc ttttttaagc
     2821 gccctgcctt cgatgatgtg cacgagatta gcctttagaa ttcacttaaa taaaatctgt
     2881 ccgttaccaa agcagacagt cacattttaa ggaggaattt tggttatgtc agtatcatta
     2941 gaagtaagta aaagagaagt acgtccccgt tcattaagaa acaaacttcg tcacgaaggg
     3001 aaagttccag ctgttgttta tgggtataaa gtagaaagta cccctatctc attcgatggt
     3061 ttgacactaa gcaaattgtt gcgtgaacat ggagcgaaca cagtcatcac gatggacatc
     3121 gatggacaaa aagtcaatac attggttcac aagatccaaa cgaacacttt taccaatcat
     3181 ttcgaacatg cagaatttct atctgtaaac atgtcggaag aaacagaagt cgaaacagaa
     3241 gtcgttcttg ttggcgaatc agtgggtgct aaatctggtg gagtgttagc tcaaaacttg
     3301 tatacagtag tcgtatctgc tacaccagac aaattgcctg aacgaattga agttgacgtg
     3361 acaaacttgg aattaggtga ttctattaca gtagccgatc ttccaaaaaa cgacgattac
     3421 aaattcgtga ccgacagtga agagcaaatc gctgctgtag tcgctcaaag tgaagaagaa
     3481 gaaagcacag gtgaaggtgg cgaaccagaa gtgatcggtt caacagaaga ataatactga
     3541 aacaatcagg tggtgttaga agcggcgggt cagcagcttt taacacgcct tttttatttc
     3601 taatcgttta ttttcatatg gggcttccac ttttgtaaat ggctttacaa acgaaaaatc
     3661 ctaatttacc aagcttttcc tcgcatactt ttatcgtacc tttataaaaa tttatacttt
     3721 gaaaaaaagt attcaattat tgttgcattt ttcctgattc tttattagaa tgaaccctcg
     3781 ttgaagtaag tatttctagt ttatcctagg attaattata aaaataaaac attcgttgat
     3841 tcgaaggagg ttaaacagat gtttgaaagt tttgatagca gccgcaaccg ttacgcttca
     3901 gttggtgtcg tatcaagttt gccagatgaa ttgattgaca gtatatggtt tattattgac
     3961 ctagatctta agggagtcat tccattagaa aatatattaa cctttgattt gatcgaaaat
     4021 cgtggaaaag tgacgatgca tttttctcaa gcaggaagca atgtggaaat ggatattgat
     4081 ttgccgtttg cctattcgaa ccaatttcct gctcaagtat acgcctatga tgacggtacg
     4141 agagaaacga tccttttacc aagtgagatt agacaatatt aaggtgatag ataaacgtgg
     4201 atgtccctta ttttacagac caggccaagt tgttgattct ttgactcaat tcttggcttc
     4261 tttttatgga gtttcataaa ttatcattga tttctaattg gtattctcag gataaacgct
     4321 atatactact aactaaattt taaagatata aggagtttaa aagatgcgcg atttaacaaa
     4381 aggaagcccc gctaaactga ttttgatgtt cactgttcct ttactgatcg gaaatgtttt
     4441 tcagcagttc tacaatatga tcgatatgat catcgtgggt cagacgctag gtaaaaatgc
     4501 gctggcagcg gttggtgcaa ctggaagcct gacatttttg atcattggct ttgcccaagg
     4561 tttaacagct ggtttagcca ttattaccgc acaacgttac ggtgcaaaag attatcgggg
     4621 tctgaagaaa agttttgctg ctagtgttgt cattagtctt gtcgtcactg tgattttaac
     4681 tgtcctaagt ctcctgttta ttcgtccgat gcttcaattg atgcaaactc ctcctgaaat
     4741 acttgatcaa gcacaaacat ttatttcaat tattttatta gggatctttg catcagtcag
     4801 tttcaatttg ctttctaatg tcattcgcgc acttggtgac agccgcacac cactattctt
     4861 tctgatcatt gcggtcatca tcaacgtcgt acttgatttg atctttatta tctattttgg
     4921 catgggtgtg gaaggtgcag ctattgctac agtaatcgca caagtttctt ccagcgtact
     4981 gtgtctagtg tatatcaaga aaaaaatgcc tttgttacag ttacgcaaaa aagatttcaa
     5041 atttgaaaaa gaagagtttg ctgtacacct aaatgctgct ttgccaatgg cgtttcaatc
     5101 ttcgatcatc gctgttggtg ctatcgtgtt acaagcagca ctaaatagtc tagggacaga
     5161 tgtcgtagct gctcaagccg ctgctggtcg tatcgaccaa tttgcaaatc agccgatgat
     5221 gtcatttggt attgcaatgg caactttcac tgcacaaaac tatggtgcga aagaatatgg
     5281 ccgaatcttg caaggggtac gtcaaacttt actgatgagt gtcggattta gtctcttagc
     5341 aggtgctatc gtcattttct tcggtcattt ctttgttcaa ttgtttgtta gcccatcaga
     5401 aacacgtgta tttgaattag cacaaaccta cttcaatatc aatgggtctc tttactggat
     5461 cttagcagtc ctgttcattt tacgctacac acttcaaggg cttggacaaa gcaagatccc
     5521 tacgatcgcc ggtatgatgg aattgcttat gcgttctttt gcagctatcg ttcttacagg
     5581 aatgtgggga tacccaggag cagcagcagc ttctccttta gcttggatcg gttctgttgc
     5641 cgtgttgctt tactcttata tgcgttcgat gaaacaattg aaacacatgg gtgaagagga
     5701 acatttcaca ttagaacatg cagaagcaag atagccttga ttaaaagaaa tcgttgcggc
     5761 taaaaatgaa aacttttatt gaaaatatct tcttcgttta ccgaaaacag gttttttaga
     5821 taacaaaaag gctgtgacaa aaaatgtcac agcctttttt cttttattct gtttttcttt
     5881 ccgtggactc tactttgaaa taatcgatat gtgcatgctg gtattctaat accgtgaaac
     5941 ggaatgatgc aatctcaatg attgttcctg cttttatttc tttatatcgg ctagaagcaa
     6001 agcccgcaag tgtagtaaaa ttcgtttctt caaactctgg aatctctaca tgaaaataac
     6061 gctggaaatc atctaatgtt agtttgccgg agatgatata agagccatct gactgttccg
     6121 ctatcaatgc ttcgtccaaa tctgtctcat ctcggatttc cccgacgatt tcttctaaaa
     6181 tatcttcaat cgtcactaag ccgcttgtcc ctccgtattc atcttttaag atagcaatat
     6241 gcttaccctt ttttcgcatg atcgtcaata atttttttat agggatcgtt tcagttgtta
     6301 cgatcggctc ttcaatcaac tgttctactg tcagctggtc atctttataa gattgcttga
     6361 tcaaatctgg caaggtgaca taacctaaaa cattgtcttt tgattctaag ataaccggat
     6421 agcgagtatg tccttgttca atcgaagctt ttatcgcttc tttgacggtt tctgaagctt
     6481 cgaatacttg catggacgta cggtctactt gtatatcttt tgccaaggta tcatcaaatt
     6541 caaacacgtt ttccaaataa taatattctt ctttggtcaa ctctcctttt tcataagaat
     6601 tcaatgcgac taatgtcagc tcttcttgag acaatgtctc ttctccttca gatacgagac
     6661 gcacacctaa tagcttccct agccctgcag ctgaatgatt caatacccaa ataaaaggaa
     6721 acattacttt ataaaaataa tgaagtggtt taactacgaa caagacgacc ttctccgtat
     6781 tgacgatact atagctctta ggaagcagtt cgccaataac cacatcgata tacgtaacta
     6841 aaataaatcc cagcgccaca gagattgtac tgctaatagc cgaactaaaa ggtagtttac
     6901 cgatcactgg ttctagtaaa gccgcgacag atccttcacc gacccacccg atgatcaaac
     6961 cggttagcgt aattcctaat tgactagctg ataaatagtt atctaaatgg tgtgtaacat
     7021 gaagagctag cttggcgttc tttacatttt ccttttctag ttgttccaat cttgatcgtc
     7081 tgattttcac taaagcaaat tcacttgcta caaaaaatga agtaattgca atcatcaaaa
     7141 ataaaataat gaaattgata accatattct cccgcctttc tctttttcta ttgtaccctt
     7201 tttctacaaa aagattgaaa ataatgcgta ttcatattta ggaataaaaa aggagatgcg
     7261 gcatttttca gccacatctc cttatcactt caaaatcatt gcgttacttg ttgctaaaca
     7321 gctttttcat gacctctgat aaaaacggta ttcaaaagct tgcatctacg gcattaagcc
     7381 caaacagatg aaaaacatga gaagctgttt ttatctgctc gttcttactg cttgttgcag
     7441 tacagctttt tcatgacctc tgataaacgg tgttcaagta gctgacattc gacattagtt
     7501 caggtcaaag cgctactttc acaaccgctc tttcaaccat gttttccgag tatttccaat
     7561 gataagcgga acagtttcat gttttacttc gctgtactct agcccaaacc atgtttctgg
     7621 cgaggtagcg atttttttga ttgccagttt tgcctgcatg atttgacgtt gccattttcc
     7681 tagagttgtc acattggaaa ctacttcatc aatcaggaca aattggaaat cgccaacttc
     7741 tcgccctggt gtcagagagt atctttgtgg ctgttttggc aaacggcctt gtttcatcaa
     7801 atcatggatg acttggcgaa catatacgtt gatttcctgt tggaccctga aacctaagaa
     7861 cagattcact tggacaataa aatcagtgtc catcatatct acttgatatt ctttcgtata
     7921 tggctcatcc gtcacttcca catgaacaaa ccaatagact cgtgctcttt taggccgttt
     7981 atctaaaatc gaatagatga actgtcggcc aactttatct tcagccatat caggaaccaa
     8041 gaagaccaca tttgtctgat agattggtaa agaagtatca tcacgtaatt ctctaagctg
     8101 cgggatataa tctctcaaag acacatcctc ggttgacgat tcgcgaataa tgttcccttg
     8161 ttcccaaata aacatcacga ctaagatgat caaaccaatc agaacggcta caaaaccgcc
     8221 tcggaagaat ttgacaacac ttgagataaa gaaaacgact tctatcgcac cgaagaaaaa
     8281 ggtaactaaa tatgcaagcc aacgagaatg cccattttgg ataagataaa agtaaagcag
     8341 tgctgttgtc atcaacatcg tcacagtgat cgatagaccg taagcagctt ccatatgatg
     8401 agacgttcgg aatgtgacaa ccactagaga acaggcgatc cataaaatca gattgactgc
     8461 tgggatatac atttgcccga tactgttccc aggatacatg atctttaacc gaggcagcag
     8521 tttcaacttg attgcttctg aaactagcgt aaacgaacct gtaatcaatg cttgcgaagc
     8581 aatgactgca gcgactgttg caaaagcaac gccgatcacg atccaacttt gaggcatcat
     8641 ttggaagaat ggattcaggt tcttgatttg ctgtgcttca gggttttgat aaacattcaa
     8701 caaccacgca gcctgcccaa aataattgat aaccaaacaa attttgacgt aaggccagct
     8761 cagacgaata tttcgtttgc cgacatgtcc aagatcggaa taaagcgcct ctgcacctgt
     8821 cgtagccagg aaaatatttc caagaatgaa caagcctaat ttgttatctg cactaaaaag
     8881 cagctggata gcataataag gattcagtgc acggataact gtccagtctt gactgaagtt
     8941 cattaatccc attacgccta aaaaagtaaa ccaagcgaac atgattggtc cgaatgcttt
     9001 tccgacaata tctgtaccaa agcgttggac catgaataaa acaaaaataa taactaaagt
     9061 gataatgacg atcacgttct ggttattacc gaaccggtca aaaaactgcg gaattcctcg
     9121 caagccttca atcgcagtcg ttaccgtcac agcaggggtc aataccccat ctgctagtaa
     9181 ggcagctcct ccgatcattg caggaatgat caaatatttc ccaccttttc ggacaagtgt
     9241 gtagagtgag aaaattccac cttcaccatg gttatctgca ttcaatgcaa tcacgacata
     9301 tttgattgtt gtcagtagag tcaatgtcca aaaaatcaat gagactgatc cgataacaaa
     9361 atcaggattg attcctgaca atccgccatt atcttcaaca atcgatttca taacgtaaag
     9421 cggacttgtc ccgatgtctc cgtagacgac tcccatcgct acaagcaggc cagtcattga
     9481 tatctttttg gtattgtgcg ttgttgccac gtttttccct catttcttgt tatatggata
     9541 tcctttaggt aagcttgaat gatagatgat attcttatcc atgacacccg ctttcctaaa
     9601 ttgcttctaa ttatccaagt aacacactga agtgtcaact tatttgaata aaattattct
     9661 ttctttggac gttctctgat acgtcttgcc acaaatgaat aaagagcaag gcaaaaaatt
     9721 gcacccaata atgctggtat caaataaaaa ccgccgagtt ccggtcccag tggtcctagt
     9781 aaaaattcgc cgagccaact acctaaaaaa ccgataatga tatttccaga tacgcctccc
     9841 gggacttcct ttcctaaaat tgcgccagct atcaaaccta atataccgcc gataaataac
     9901 gaccataaaa aattcagcat ctcctttccc tcctttatcc atatttctca cttttatttt
     9961 acgctttatt cagaaggact ccaaaaatat gcgccaagaa actccagaaa aaaagaagtt
    10021 gtaacaaaag tgttcagctt atttccgtag aagctgcttc tggaacaccg tgaatcagag
    10081 gttgtgaaat cagcggtttg acctgagtat tagagaaaca aacggaaaaa tagttcctca
    10141 tattttgccg ttttttgagc taatatcgag ggacagctga ttgaacatcg tttattggag
    10201 aggttgtaaa aaagctgttc tgcatcaagt aataagaaca gccaaataaa aacagctcct
    10261 aatgttttta acttggctgg acttattacc gcagatgcaa gcttttgaac accgtttatt
    10321 cgaaaaaaaa ggactgtgac acggcttttg tcacagtccc cttttttcga ataattattg
    10381 tccccctgtc accttcacga tcacaagtac attattccta tgaattaaat tatttttctt
    10441 catttttcgt tgaaccatct tcgttcaaca ttgcttttct tgacagattt acgcgtcctt
    10501 gtttatcgat ttcagttact ttcacaagga tctcatcacc tagtttaaca acatcttcga
    10561 ctttattgac acgatcgttt gctaattggg aaatatggac aagtccatct tttcctttga
    10621 tcaagttgac gaatgcaccg aatttttcga tacggacaac tttccctaga taaacttcac
    10681 caactttgac ttctttggtc aaatcttcga tgatcttgat agcttttttg atcatttctg
    10741 catcagctga agcaatactt acttttccat cttgatcgat atcgattttc acaccagttt
    10801 catcgatgat tccgttgatc gtatcgccgc ctttaccgat aacatctttg attttagcag
    10861 gttcgatctg gatcatttcg atttttggtg catattggct taattcttca cgtggagcag
    10921 caatcgttga agtcaattct tctaagattt ccatacgtgc ttgttttgct tgagttaatg
    10981 cttcagttag gatttgttct gtgattcctt gaatcttgat atccatctgt aaagcagtga
    11041 taccatcttt tgtaccggca actttaaagt ccatatcgcc aagatgatct tccagccctt
    11101 ggatatcagt taggatcgta tagttttcac catcagaaac aagtcccatc gcaataccgg
    11161 cgactggcgc tttgattggt acacctgcat ccatcaatgc taatgtgcca gcacagatac
    11221 ttgcttggga agaagaacca tttgattcca atacttctgc tactaaacgg atcgtataag
    11281 gaaattcttc ttctgaaggg attacttgtg ctaacgcacg ttcacctaat gcgccatgtc
    11341 cgatttcccg acgccctgga gagcctgcac gtcctgtaga accaacagaa aattgaggga
    11401 aattgtagtg gtggatgaat cgtttgctga cttccacccc taaaccatca atgatttggt
    11461 gctctcctag aggtgcgagt gtacaagctg ataaagcttg tgtttgtcct cgtgtgaaca
    11521 aacctgatcc atgtacacgt ggcaataagc tgacctctga tgacaatgga cggatttcat
    11581 ccaatttacg cccgtcagga cggatcttat cgatggtgat caattcacgt acgacatctt
    11641 tttcaagatc ttctgtgatt tgtttaactt ctttcaacaa gtgatcttca tcttcatgac
    11701 ctgcaaattt ctcagcataa gcttcgcgga tatcgatctt cacctgctcg atattatctt
    11761 cgcgagcaag tttttcttca gtcattactg ctgctttcat tgtagcgtaa gaagcatcaa
    11821 agatttcttt tttcaatact tcatcaactt gcagtaaagt cacttccatt ttttctttcc
    11881 ctactgcttg aacgatttct tcttggaaag caactaactc tttgatagcg tcaaaaccaa
    11941 acagcaatgc accaagcatg tcttcttctg aaacttcttt cgcgccgctt tcaaccatat
    12001 tgatggcttg tttcgttccg gcaacagtca attcgatatc tgtttgttct gcttgttcta
    12061 ctgttggatt caagacgtac tcgccattta cacgaccaac ttctacgcct gcgattgggc
    12121 cgtcaaatgg gatatctgag attgatagtg ctaaagaaga acctagcatt gctgccatag
    12181 ctggcgagca gtctgtttct acactcatca cgatgttcgt tacttggact tcattacgga
    12241 agccttctgc aaacatcggg cgaattggac ggtcgatcaa acgggcagtc aatgttgctt
    12301 ccgtacttgg acggccttct cgtttgatga aacctccagg gattttccct gctgcataca
    12361 ttttttcttc ataattgatc gttaatggga aaaagtctgt atcttttgct tctttagagg
    12421 ccacagctgc acttaagact actgtatctc cataacggac taaaacggct ccgtttgctt
    12481 gttttgctag ctgaccgact tcgatttgta agggacggcc gccccatgtc gttttgaata
    12541 cttgtttttc tgacataact tctccttttc cttctcgtga aagcaagacg ctactaaaca
    12601 aatgaaaact gccgaaagcc gtcttttgag cagctttcat ttggttagta ggagtccatc
    12661 tagcttgatc acggaatttt acttaattga tatacgagta aaaaaagtga gattcatgtg
    12721 aatcccactc tttttgacta aaattaacga cgcaagccta aacgtttgat caattcgcgg
    12781 taacgttgaa cgtcagtttt acgtaagtaa gctaacaagt tacggcggtg accaactttt
    12841 ttcattaggc cgcggtatga atgatggtct tttttgtgca cgcgtgcatg ttcattcaag
    12901 tggttgattt cttcagttaa tacagcgatt tgtacttctg gagaaccagt atccccttca
    12961 tggcgagcgt attcgttgat gatttcattc ttgcgttctt ttgaaattgc catttctttc
    13021 acctctttcg attattcccc tgcgactgag taaaacgttg gtgattcgct taaccaagtg
    13081 gcaggtcatg tgcctagagc acacagctat actttacctg aaaccgggtt gaaaatcaag
    13141 tagttaatca ttaagaaaag gtagttttca aacaatctta ctactacttt tccatttttg
    13201 tggtaaacta taatgcgaaa atagacttga aacacagtct tttaagaaag gggtacatat
    13261 tccatgatta caatggatga tatcattcgt gaaggaaatc cgaccttacg cgaagttgca
    13321 aaagaagttt ctcttccatt aagcgaagaa gatatctctt taggaaaaga gatgcttgag
    13381 ttcttaaaaa acagccaaga tccaatcaaa gctgaagaac tacacttacg aggcggtgtt
    13441 ggcttagctg ctcctcaatt agatatttct aaaagaatca ttgctgttca cgtaccaagt
    13501 cctgacccag aggcagatgg accttctcta agtactgtta tgtataatcc taaaattttg
    13561 agccattctg ttcaagatgc ttgtttaggg gaaggtgaag gctgtttatc tgttgatcga
    13621 gaagtaccag gatatgttgt tcgccatgcc aaaatcactg tgtcttatta tgatatgaat
    13681 ggtgaaaaac acaagattcg tttgaagaat tatgaatcga tcgtcgttca acatgaaatc
    13741 gaccatatca acggtgtgat gttctatgac cacatcaacg atcaaaatcc atttgcacta
    13801 aaagaaggcg tattggtgat cgagtagaga attggcaggt tccttcgata ctatgctaca
    13861 agaaagcttt ataaaaaagg ggagtgctca aaagctgcac ttcccttttt tatgtttctc
    13921 cactaatcga aatttagata gacatctaga aatcgcaatt ccaaaaaatt ctgtgtattg
    13981 gatacgcatc ttttcttatt ttttcttatt tcttccatat gggtgtttat ctcagagatg
    14041 atagaatagt ttctaggata aaaagtagca gctacgatgt tatccttcgt agaggattcc
    14101 tgcttcagct agtttttgtt tgatgttttt cagcatatgc ttttctgggt agcggatcgt
    14161 atgcagatgg atgccgcctg ttaattcgga taacaaagta gcattacttt tttgataacg
    14221 tctcataaag gaatcgatat cttgtggcgt tttgatgtgc aaagttcctg ctaactcacc
    14281 gtaaagggga tgttccacaa ttacatcaat gatttcacca ccattttcaa cgattagtct
    14341 cagttcttct tctgtttgat cttttccatg ctggacagcg attttactga tgtgcccagt
    14401 attggtttct tctgtttcga ggatataacc tcttgctgtt gccacgatac ttttacctgc
    14461 agctcgtagt aaagcaatat cgcctacaat gatctgacgg ctgactccaa aagttttcgc
    14521 aaacttgcta gcactcactg gtttttctgt atttgccaag gtttgcatga tttgttttcg
    14581 ccgtgtctct ccttcgatca cacttattcc tccttcattt tgttttggta ttcgcgcgag
    14641 tgtttcatca aatcaacata cgtgtctgtt attgtttcct tatatccctt atttttcagc
    14701 agactaacaa ctttatccaa tacttgctgc tctctttttg catcataaac tgcttggttt
    14761 tcttcttttt tcacttgtcc tattttagtt accgtatcca ttcttttttc taataaagca
    14821 accaactctc gatcgatcat atcgatctta tgacgtaatg tttcaagaga ctcttctgtt
    14881 agttcttcct ctactgtctt gatgatacga gcaggatttc ctgcaatcac tacattgtcg
    14941 ggaaaagatt tggtcacaac cgaacctgcg ccgactacta cgttgtcccc tagagtgaca
    15001 cccggcgtga cgataactcc tccgcctaac caaacattat taccgatttt aatgggtttt
    15061 gcgtactcca gaccgctgtt acgtttcact ggatgtagtg gatgcgtcgc cgtgtaaagc
    15121 tggacattcg gagcgaacat gcagttatca ccgatttcaa tcgtgctgac atctaaaaat
    15181 gtacaattga aatttgcata aaaattctcc ccaacaaata tattatatcc gtaatcaaaa
    15241 ttgataactg gctccatata gatttttttc cctgtacggc caaaagtctc tttcaacaat
    15301 tgggatcgca gttccgctga ttctgcttgg ttgatgattt ggctttgact tcttgcaaac
    15361 atacggtcgg caaccagctc aggatcattg gcaaaataaa gctcgcctgc aatcattttt
    15421 tctttttcgg ttttgaattc agtcataatc tgctcctaat tcttgtattt ttttgaaaac
    15481 aaccgctccg tcttcttgaa taaacgtata aggctccgct aaataaacag gcgtttgatt
    15541 gattactaag attttggctg acggctggcg aaactgtacc agatcacaaa acggatgaac
    15601 ttgaaaactc gttccaacaa tgacgatcag ctctgcttgg ctgacggcat aagccgcctg
    15661 ctccatgact ccatcttgaa acccttcacc atataacacg atgtctgggc gtatttgtcc
    15721 tccgcatgct tgatgtttgt cgctgtgaag atattctttc cagtctaccg tttgttgaca
    15781 ttttcgacaa tgacattgat acagactccc atgaaaattg acgagttgcg ggcttttagc
    15841 ctttgcgtgc aaaccgtcga tgttttggga aacaacccaa acattttttt ctttcgctag
    15901 atgtgctatt tccaaatgaa tgatattggg ttttgcttca ggatgataaa tctttttgac
    15961 aaattgatag aatttatccg gttcattcac cattgcttga tgactcagca agtattccgg
    16021 ctgctccatc ccgtggtaga ttcctgacaa agatcgatag tctggcactc cagaaggtgt
    16081 ggatacccct gcaccggtca aaaatgtaat ttgctgataa gctaatatct cttttgctgc
    16141 ttcgtctact gttaattgtt tcatgttttg tcacctcact ttccattata gcccatactc
    16201 gaacaaattt attgttgaaa agtcagaaaa atgggaataa aaaagtgtta tgggcatttg
    16261 agcaatttat cacaatgttc actgacaaaa gaatgaaaac gtgttacaat actgctgtat
    16321 aaaagtgagg tgaatcgata tgttaccatt ctttatttat tggggcgtta ttctggcttg
    16381 tatcgcctgg ttagcgttaa gcgtttattt ctctgttttt tatcttgtac gcaaagaaaa
    16441 tggaaatctt tgggcttttg ctttattcaa cgtgattgcc gcaattgttt tagccattac
    16501 attagccgta tatcgaacat ggggctgggg aatcacccaa tactcaagtt tgatttatct
    16561 aatattagcc atttatggcg tcgttgttct tttgcaagcg atcctaggac gtgagccaaa
    16621 gaaagcggca gcttaaatga tcgagatgga actggacaca ggaaattttc tgttctagtt
    16681 ctttttttat tgtattacat acatttttgt gtataattat actatcgttt gcaatggata
    16741 gatgattcac attttgattg ttttttcggt aaaagtaaag atttctatat ttatcagtat
    16801 tttttcaaaa accagcagtt atagattgac aaagcctctc gagtatcgta aacttttttt
    16861 gtataaataa ctgaatacga gatgcctcaa tcaatgaggt agaggtcgcg agaatcatta
    16921 accctgtgga gtgaagcaaa catggagaag caggaatagg gaggatcgcc gaaatgtgca
    16981 cttttttgct tggagtgtat gttgggacgc aagttaagag cttgcggact gtcttagcag
    17041 tgatgctaag ttgcgctatg ttttgtgaga ggtttatcac cttatggaat tttagcggcc
    17101 tttttatatc tataaatcgg tcgcttttta ttttttcaga taaagcttta ccgcatttca
    17161 tctagaccgc tcgtgtatca tataatcaga aagggagaaa aaaatgaaaa aaaatatggt
    17221 ttcagttgcc gtactatttg cttttgtctt ctcactattc agtgtgggat ctctggcaaa
    17281 tgcagatgaa aaaacaccga gcgatgagtt tcgcgtaggt atggaggctg ggtatgcacc
    17341 cttcaactgg tctcaacaaa cagatgagaa taatgccttg ccaattcaag gccaaaatag
    17401 ttacgccggc ggttatgatg ttcaaatcgc taaaaaagta gcagatggtt tagggaaaaa
    17461 attagtcatc gtccaaacga aatgggatgg tctggctcct gcccttcaat caggcaagat
    17521 cgacgcgatc atcgccggaa tgagccctac tgctgaacga cgaaaagaaa ttgattttac
    17581 cgatccttat tacgaatcac aattagtagt agtagttcaa aaagatagca aatatgcaaa
    17641 agcaaaaagt ttagcagatc tttctggagc aaagatcact gctcagttaa atacattcca
    17701 ctatactgtg atcgatcaga taccagatgt gcagaaacaa caagcaatgg ataatttctc
    17761 agcaatgaga acagctttag cttctggaat gatcgatggt tatgtcagcg aacgtccgga
    17821 aggtgtaacg gcaactagtg tgaacaaaga cttggaaatg cttgaattca gcggtgcaga
    17881 tggcttccag acaaacccgg aagatgttca gatttctgtt ggtatgcgta aaggcgaccc
    17941 ggatatccaa aaagtaaacc agattctttc tggcatctct aacgatgaac gaaccaaaat
    18001 tatggaccaa gcagtcaaag atcagcctgc tgcaactgat agtgaagaaa aacctggcat
    18061 catttctgat ttcactacga tttggaaaca atacggcaat atgttcttac gaggtgctgg
    18121 acttaccttg ttcatcgcat tgatcgggac agttgtcggt actacacttg gattactgat
    18181 tggtgtcttc cgttcggtac ctgaatcagg aaatgcactg actcgcttct tccaaaaaat
    18241 cggaaatgca cttctttcta tctatgtcga agtattccgc ggaacaccga tgatggtaca
    18301 agcaatggtc atcttctacg gtctggcact cgcttttggc atttcattga accgaacgat
    18361 tgccgctttg tttattgtct ctctcaatac tggagcttac atgacagaga tcgttcgtgg
    18421 ggggattttt gcagtagatg aagggcagtt cgaagctgcg caagccattg gtatgaccca
    18481 tggccaaacg atgagaaaag ttgttattcc acaagtttta cggaatatct tgccagcaac
    18541 aggaaatgag tttgttatca atatcaaaga tacagcagtt ttaagtgtaa tcggcgtagc
    18601 tgatttgttc ttccaaggaa actctgcttc cggtgcgaat ttccaattct tccaaacatt
    18661 tacgatcgtc gggatcatct acttagtcat gacctttacg atcaccagaa tcctgcgggc
    18721 ggtagaaaag aaaatcgatg gtccttcagc ttataccaag cttgaagaaa tcgacaattc
    18781 aaatttgcag gaataaggag gaagaatcat gaatgaaaaa attattgaaa tcaaacatct
    18841 aagaaaaagc ttcggtgaaa atgaagtatt gaaagatatc agcgtaacag tcaataaagg
    18901 tgaagttgtg acgatcatcg gttcttccgg ttctggtaaa tctactttct tgcgctgtat
    18961 caatttactg gaaaaaccaa caggcggcga gatcatttat aatggagaaa atgtattagc
    19021 tcctaaatat aatttaccta aatatcgaac gaatctaggt atggttttcc aatccttcaa
    19081 cctgttcaac aatatgaatg tcttggaaaa ctgtatgtct ggtcaaacaa ctgtcctgaa
    19141 acgagataaa gaaactgcca aaaaagtagc aatcgaaaat ctggaaaaag ttggtatgga
    19201 tcgttatatc gaagccaaac cagcacaatt atctggtgga cagaaacagc gtgtcgcaat
    19261 tgctcgggca ttatcaatga atccagatgt attgctattc gacgaaccta cttcggcgtt
    19321 agaccctgaa atggttggcg aagtgcttaa tacgatcaaa gacttagcac atactggctt
    19381 gacaatgctt atcgtgacgc atgagatgga atttgcaaaa gatgtatctg accgtgtcat
    19441 ttttatggat aaaggtgtga ttgcagaaga aggtacacca gaagatatct ttgtccatcc
    19501 gaaagaagaa cgtacaaaag aattcctttc acgtatttta aatgcttagg atcaatagct
    19561 gattgggaca aagagctgcg gtgtttaccg cagttctttt ttatgaatta ctcataaaag
    19621 gtaaatttct gaaaattttg gaatcttagt tgtgtttttc agaaaactct cttaatatgg
    19681 aagtaattaa acttttttag ttaggatgat ttaaaaatga atgaagagca agaactgcag
    19741 cgtggactga aaaatagaca tgtccaactg atttctattg gcggagcgat tggaacgggg
    19801 ttgtttttag gttccggaaa gtctatccag ctagctggcc cttcgatatt gctcgcctat
    19861 cttattactg gatgcatctg ttttttcatt atgcgtgcgt taggcgaact tctgttatca
    19921 aacaccaata accattcttt tctggatttt gttgcagaat atcttggcaa aaaagcggct
    19981 ttcatcactg gctggacgta ttggttctgc tgggtatcga ttgcaatggc cgatttaaca
    20041 gccatcggaa tgtatgtacg ctactgggcg cctggtgttc ctcagtggct tcctgaactg
    20101 attgcattag gtcttcttct ttgcttgaat atggtggctg tctcattgtt tggtgagtta
    20161 gagttttggt ttgcattgat caaagttgta gcgatcattg cctttatcct cgtaggagct
    20221 tatatgatcc tcacccacta tacgacagat attggcacag ccagtatcac gaatctatgg
    20281 tctcatggcg gattctttcc aactggtgca aaaggattta tcttagcttt ccaaatggta
    20341 acctttgctt ttgctgggat agaattagtt ggattagtcg ctggagaaac agaaaatcct
    20401 gaaaaagtgt tgccggaagc tatcaataat attcctatca ggatcatctt attttactta
    20461 ggatctcttt ttgtcattat ggcaatctat ccatggaaca gcttgaatcc agataacagt
    20521 ccatttgtag aagtattttc ggaaattggg atcacgattg ctgcttcctt gatcaattta
    20581 gtcgtattaa gtgcagcagc ttctgcctgc aacagtgcaa tatatagtac aggacgtatg
    20641 cttcgttctt tagcgcaaga aggcagtgcc cctaaaaaat tcaaaaagtt aacaacacat
    20701 catgtgccag ggaatgcttt gacattttct actatcgtca ttttcatttc tgttatcttg
    20761 aattacgtga tgcctagtga agtattcaca ctagtaagca gtattgccac tacttgtttc
    20821 ttgtttatct ggagtatgct tgtctacacg cacatgaaat atcgtaaaag tatcctcggc
    20881 aaaaaagctc attcatttaa aatgccgttg tacccattta gtaattatct ggtatttctt
    20941 tacatggcct ttgtgtgtgt cgtcttgttc ctaggaaaag atacgagaat cgctttatta
    21001 ctaacacctg tgtggttctt gcttctatta ctaatctatc atatgaaata tgaacgtaag
    21061 aagtaagaaa gtagttgtga cagaagtggt cagcttcgag aaataagaag gaatttacga
    21121 aaattgcttc ttaaattttt gtgaaatttc ggcttatttc ttggagataa aagcttctgt
    21181 cccagcctct tttctatttc cccggaaaca accgctgata ataggcatct gcttttttat
    21241 aggaaaaaat ccaaatacca gatacaaaaa aacgtccgaa taacccgatc aacggtacaa
    21301 aaaagacacc ggcaatcaag cataagttaa tcatcatgga gataagactt cgacggtcct
    21361 ttgacaaaac tctcacgaca tgggaatctg gtttatttgc tttcatgatt tgcctcatca
    21421 agataccaaa agaaaaactc cacatcgtgt atactaagac ataagccatc tcaggccaaa
    21481 aagcttcagg gttacgcccg acccaactag taacagaagg acataaagtc agccaaaaga
    21541 gcaaaaagat attcgaccag accactcggt aattaattcg ttctgccatc tgaaatatac
    21601 gatgatggtt cacccagatc accgcaacaa atataaagct gatggtataa gctaataatg
    21661 tatttcggat gacccataaa gaatgaaagc tagatgtatt agggatcttg atctctaata
    21721 caataactct caaaacaatg gctaccactc catcagtaaa tgcttctaat ctcgactttg
    21781 tcacgatgat tcctcctcag atgattcagc gcttttattt tatcacttac gttattaaat
    21841 aaccatcgtt ttatataggg aattttctga aaattgttgc atatttttcg aaattttgtt
    21901 acttttaaaa a
//
LOCUS       DACDXO010000048        20982 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E23605_2017
            SAMN12639989-rid9881463.denovo.049, whole genome shotgun sequence.
ACCESSION   DACDXO010000048 DACDXO010000000
VERSION     DACDXO010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639989
            Sequence Read Archive: SRR10023389
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20982)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20982)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741914.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:42:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,972
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 33 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 17 of 73
            Pseudo Genes (multiple problems)  :: 10 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20982
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639989-rid9881463.denovo.049"
                     /isolate="E23605_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(27..2630)
                     /locus_tag="IWM35_001940"
     CDS             complement(27..2630)
                     /locus_tag="IWM35_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HAP9919785.1"
                     /translation="MVNAHIIQHTHWDREWYFTNEDAIVLSDQVFTEVLDELERNPKV
                     NFCLDGQTSIVDEYLEINPQKLKVIQRLMKTGQLFVGPWYAQTDALLVDAESILRNLM
                     IGISDTILKYGDPMMVGYLPDTFGFNANLPTLLHQVEIDNFMCWRGLNFETMVPSPYF
                     IWKGLGDKFVYAMNFPFGYMTGMMTLDAIKDIPEFAKEKLDEAVEMLHMQGKNKDILV
                     PSGVDQKSMILNFDQVVEKLNKYSKFHNIISDYPSYVEVIRNKKELPEYKGELRQPVY
                     SRVHRSIGSVRTKMKIENFKLEQKILKRVEPLMLIAERNGIHISKGLLRRLWKKILQN
                     QAHDSIGGCVSDNVAEDIFHRIKEANEIADGIENLIVKRMADALKLKSNEVLVFNTTT
                     ENYSGSKIVHVVADSKKIHFKGSPYNIIEKEVYFPERKNAYRMIATGFEYFDEPAYYE
                     LDIKINVNVPALGYTVVEFEKNNEKLETAIEIDKTEISNNKYKLSFKDGQLNLIVGDR
                     QYLDFVHLIDSANDGDTYDYSPLEGDTELSLKLETAKVYKDSLQETLVVYGKAQLPKD
                     LKDRLSEKPEMEEISYEISFSLGESQIVEGTLKIHNTVYSHRMRLAINVLDKSEKSIA
                     QIQNGFIENTPDVIPPNWSETMVEKPVNLEIFDKSVSVAENDHYFTFYADGLKEYERI
                     ENQLIITLFSTTGELGKPNLAWRPGRASGDTTNEGHVMMETPLAQEIGEYKVTFGFNV
                     EEGRFNEFKVAKQAEKRLEQSISYQKQKLNVFIHRLDNKIWPLQNELDVPRKFSLIEL
                     PEDLIVSSIYPSYFHEQGFVLRLANPTEQEKIVPEAILSLGTVVNALENKQELTTIPP
                     YDYLSILINER"
     gene            complement(2642..3691)
                     /locus_tag="IWM35_001941"
     CDS             complement(2642..3691)
                     /locus_tag="IWM35_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HAP9919786.1"
                     /translation="MEFKKQGREILNHFQSGVSYMIPLVVAAGLLTSIAVIFGGTGVW
                     DQTDTFWGVLRMIGQTGLQFIVPMISAYIAYSIADRPGLAPAFITGMMCQNLGMGFIG
                     GMVAGLACGYLANALKKVKVPMQVISLKSIMLIPFVTTLVVGIILWYVLATPIQAMTT
                     GLTNWLEGMSGANAALMSAILGGMMAFDMGGPINKIAYAFGVASFSSGSFGPSTAMLL
                     GIAIPPFGMFLATILNKKLYDESERDNAKTAMIMGLVGITEGTIPFAVKDPLRVIPSI
                     VVGTAVACGLNGFFGVTQETMLSTFMAIPFVTNIPLYLVSIVIGGVVTALMVNALKSI
                     KYKKEQQVNSVEEGK"
     gene            complement(3717..4031)
                     /locus_tag="IWM35_001942"
     CDS             complement(3717..4031)
                     /locus_tag="IWM35_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HAP9919787.1"
                     /translation="MTKIVAVTACIAGIAHTYMAKANLEKFAKKKGLDIKVETQGAMG
                     IENKLSISDVSEADVVIFAVDTNVSERGRFDGKPILTVGTSEVVKDGEETIDEAIALI
                     NK"
     gene            complement(4047..4493)
                     /locus_tag="IWM35_001943"
     CDS             complement(4047..4493)
                     /locus_tag="IWM35_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAP9919788.1"
                     /translation="MISEKININNIVIDLNASDKKSVVEKLSKLLETNGFVSDHKQFQ
                     KDVFDRENHMTTGIGKNIAIPHGKSLAVIESTVAVAKLKRSVEWGALDDQPVDLVFLL
                     AIKDTDKGTNHLRILAELSGRLMDDEFVKRIKDSRNKKELVKALSF"
     gene            complement(4494..6470)
                     /locus_tag="IWM35_001944"
     CDS             complement(4494..6470)
                     /locus_tag="IWM35_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HAP9919789.1"
                     /translation="MKIEIIIVHLSCIQEVKIMGIEFSERQMKILYFLIENEHTTYKE
                     LSEQLYVSTKTISKDILILKEIIQKMPDVQMLVQPKKGVSLIGKKETLRNLLSSHKIN
                     IADSPEDRLTYVFAKLLNTNDSLKIQNLADELYVSRSTVESTMKDVRKRLNKHGMSLV
                     SDRNGVRIKADEKSKRRLMSEVVNYYWGGFTASNSKTDLLDLNINFIGEANNFLNKDI
                     LKKVAELLNRYMNKTQLKFTDYEFQTLAIHLAIAIERIETSNFLSRSNNSEEEISKNT
                     LILIKMIEKAFSIIVPIDEQAYVDIHITSIEKNAMNHDLDQTKLSYLDMGVELQELVR
                     IKLNEFYPDEELINNLVLHLNAAVKRLKLGVNIYNPYTDKIKYSFKRSFMISVDLLEE
                     IEERFCIHFNEDEIAYVTLHIQSLLDRYKPDKTKVILVCSSGYGTSKLLEQRINNGFA
                     DMVEIEDVLSINELQDRKVTDELVISTIPIENTNFPVIVVSPLMLGADVRKVKQYLQL
                     NEQTEDAFLNLLDKRFCFFTHQIKDNQVNVLNIITKKLVKENYAKEGILEGAIKREKL
                     SSTAMNDFAMPHIEVDFIKKPVICVYVNPEGIDWDGTLVHSVFFFALNEKVKPELNKI
                     YETFNEIASNHELLANLKTSSSFEKLVYILKEGL"
     gene            complement(6628..7524)
                     /locus_tag="IWM35_001945"
     CDS             complement(6628..7524)
                     /locus_tag="IWM35_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP9919790.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWLDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDPFIRLTVANPIKPGTQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            complement(7517..8197)
                     /locus_tag="IWM35_001946"
     CDS             complement(7517..8197)
                     /locus_tag="IWM35_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9919791.1"
                     /translation="MVLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            complement(8198..8980)
                     /locus_tag="IWM35_001947"
     CDS             complement(8198..8980)
                     /locus_tag="IWM35_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP9919792.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMLAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LTLGTLSVPVALTFITRSTSVSLLLSFLYIMGTAFIPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            complement(8973..9890)
                     /locus_tag="IWM35_001948"
     CDS             complement(8973..9890)
                     /locus_tag="IWM35_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP9919793.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            complement(10054..10377)
                     /locus_tag="IWM35_001949"
     CDS             complement(10054..10377)
                     /locus_tag="IWM35_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP9919794.1"
                     /translation="MFSFAFYMVWLQREVPTHIQGRLMSTITTITMVASPLGLALFGA
                     LFDWKTTQPRLRDSLIFLGGSLAIVILITGLVKLLKLDLKEAKIFSEEEYRETTLVEK
                     KESGN"
     gene            11165..14392
                     /gene="fss3"
                     /locus_tag="IWM35_001950"
     CDS             11165..14392
                     /gene="fss3"
                     /locus_tag="IWM35_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HAP9919795.1"
                     /translation="MYKKQKKKLCVLTILSLAVQAIVGAIAPTITFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGFKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSDKAKLVSVLWKKVGTDADTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     EAKINQAIDDYQKKPTFHNSTAKTVLGQSTTVTDTNNLNLSEFDEVVENAANIDYRVN
                     GNQLIITPNANSKESGVLTLKKSAGTGTPVAYKKAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKESGDVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGTKVTIT
                     EKSVPAPYTIDTTPMTTTIKAGETIYVTSKNAREKGQIILDKSGVETGSDLWNDNYSL
                     AGNTFAIRKDSPTGEIVQEMTTDENGHAETPKEIANALELGTYYVTETKASHGFVNTF
                     KPVKVELKYANQTVAPVTSNVKGQNQEVTGETTLTKEDKDTGDKAQGKAVFEGAEYTL
                     FTAKDGKAVKWSEAFKPELAKGTKGSDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYSLDETKYPVSIKKVDDSEKNAVITRNVTAKEQVIRFGFDFFKFAGSAAGTTETGF
                     NDLTFKVSPLEGTNEITGAEDEATTAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKEDFVKSEYVFTITEQDQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLATWKDGNKELTSLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     DATKAAQEKDEKAKPVVIAETSATLANKEKTGTWKIQHKLTAEQVLNKTIVLFNYVYE
                     NKEAFEAGDKPVAKDVSLNNQAQTVSCTVEHHVSIQTKAHLENGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDGTNKEIWKSGKIDYEVNDKEFTKTVLAKKVDT
                     GKYPEGTKFTFAEINYDKDGNINGKHNEDLKEKSQTLTPKEVPTILSTPKQPETPTVP
                     SGSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            14505..14819
                     /locus_tag="IWM35_001951"
     CDS             14505..14819
                     /locus_tag="IWM35_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAP9919796.1"
                     /translation="MELKFVVPDMAETFGKLSYAGEGEIITEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            14832..15206
                     /locus_tag="IWM35_001952"
     CDS             14832..15206
                     /locus_tag="IWM35_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAP9919797.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTIGNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIVDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPNQGNPTNQNERKK"
     gene            15216..15551
                     /locus_tag="IWM35_001953"
     CDS             15216..15551
                     /locus_tag="IWM35_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HAP9919798.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLVKALTSDEKMFLIIHQGLL
                     GENSDSEEDSDVLDWLGEEESFFIVVEVNENLCRQAESILEEIGVEMPDAIEGFLNQL
                     VETKQLPSS"
     gene            15634..16974
                     /locus_tag="IWM35_001954"
     CDS             15634..16974
                     /locus_tag="IWM35_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010759325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HAP9919799.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQEFRQFDI
                     SKLSLSTIEIVWSIKDLVCLLGSLAFSGAMILLYIHFFLDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTRETISYFPKICYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDMIANRIGIDEVVAENGTLRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFILDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMARSKAMKEMSNYKTGENYAYLGLPPNFLIFDEYVAYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVEKNFFMKHIKGRGYVDTGGSVISEFYTPLVPKGYDFLDSISKLEKMND"
     gene            17052..17726
                     /locus_tag="IWM35_001955"
     CDS             17052..17726
                     /locus_tag="IWM35_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP9919800.1"
                     /translation="MESNDSYLEYAKANKDKFISYIIKGKSPEVGRDAVFMAGSPGAG
                     KSEVAIGLAENYDNHVIIDADYFRTQFPDYNGKNSSLFQKASSWLVEQAFRYVLDHGY
                     SFILDATFAILSAEKNISRALKKHFRVTIFYVYQDPKIAWKFTQEREIAEGRHVPKKA
                     FINAFFKSRENIAKVKSKHPDVLLHIIIKDYTNNISEVHYATDNLELFLPLGYSRKDL
                     EVQLHD"
     gene            17959..18393
                     /gene="dcm"
                     /locus_tag="IWM35_001956"
     CDS             17959..18393
                     /gene="dcm"
                     /locus_tag="IWM35_001956"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HAP9919801.1"
                     /translation="MRFLDLFAGIGGFRLGMEQASHHCIGFCEIDKFARRSYKAIHDT
                     SKEVEMHDITSVSDEFIQSLGPVDILCGGFPCQAFSIAGKRQRFSDTRGTLFFEIARF
                     TTLLRPKLLFLENVRGLLNHEGGATFETILRTLDGLGYDVEW"
     gene            18394..19101
                     /locus_tag="IWM35_001957"
     CDS             18394..19101
                     /locus_tag="IWM35_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HAP9919802.1"
                     /translation="MLNSKAYIPQSRERIFLIGHSRDACTEQVFPIIGPSSTSDQNIR
                     NLVNINPSNRGMGGQVYGSDGVAPTLTGDEGIKIALPARDGISVAGLLPGKFEQGNRV
                     YEVTGTAPTLSTKQGGTKIMIRKNETEYQEVKPEDSVNLAFPNSTSRRGRLGKQSVHT
                     LLTGDQQAIVTDQYQIRKLTPRECWRLQGFPDWAFDRDSQVNSDSQLYKQAGNSVTVP
                     VIFDIARRLKELDVHGL"
     gene            19091..19381
                     /locus_tag="IWM35_001958"
     CDS             19091..19381
                     /locus_tag="IWM35_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002395166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9919803.1"
                     /translation="MVYDELIGEIYWVIGKIQSDPELEEQLRRLKFDIRKNGVKVAGD
                     PYLMNEETDARIEINQVIAEFERIADQAKEPDIRQYLLEMKAELEVNGITDE"
     gene            19640..20824
                     /locus_tag="IWM35_001959"
     CDS             19640..20824
                     /locus_tag="IWM35_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAP9919804.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATACGLSRPYLNQIENGGV
                     TASIKTMRKIFDQLESFNPDLPLTLLFDYVRIRFPTTDARKIIQEILHLKFEYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPELTKKCQKEECISLFRTFKSYRSGELLKADEKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAFHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNRKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNRTTYIQDMVQNARLSDRHKKILEQQSMAIENL
                     II"
     gene            20839..20958
                     /locus_tag="IWM35_001960"
     CDS             20839..20958
                     /locus_tag="IWM35_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HAP9919805.1"
                     /translation="MGIVKDIFLVFGGSMLGVTVMCLMNASAAADRAMEKKGK"
BASE COUNT         6563 a         3921 c         3958 g         6540 t
ORIGIN      
        1 gtgttttttt cttgtatatt acatatttat ctctcattta tcaaaattga caaataatca
       61 tagggaggaa ttgttgttaa ttcttgttta ttctccagtg cattgacaac tgtcccaagg
      121 gaaaggatag cctcaggtac tattttttct tgttctgttg gattagctaa gcgaagaaca
      181 aatccttgtt catgaaaata agaaggataa atcgatgata caattaagtc ttcaggtaat
      241 tcaatcaatg aaaatttacg aggaacatca agttcatttt gtaatggcca aatcttgtta
      301 tctaacctgt gaataaatac attcagtttc tgcttttgat aagaaataga ttgttctaac
      361 cgtttttctg cctgttttgc cactttgaac tcattaaatc ttccttcttc tacattaaat
      421 ccaaaagtga ctttatactc acctatctct tgagctagcg gtgtttccat catgacatgc
      481 ccttcatttg tcgtatctcc tgaagctcgc ccaggtcgcc aagcaagatt aggttttcca
      541 agttcccctg ttgttgaaaa taaggttatg attaattgat tttctatacg ttcatactct
      601 tttaaaccat ctgcataaaa cgtaaaataa tgatcatttt ctgccacaga gacactctta
      661 tcgaatattt ctaaattaac aggtttctct accatagttt ctgaccaatt cggaggaatg
      721 acatcagggg tattttcaat aaatccgttt tgaatttgtg caattgattt ttcagattta
      781 tctaaaacat taattgctaa gcgcatacga tgcgagtaaa cagtattatg aattttgagt
      841 gtgccttcaa cgatttgact ttctcccaga ctgaacgata tttcataaga tatttcttcc
      901 atttcgggtt tttcagataa acgatctttt aaatctttag gaagttgagc ttttccgtaa
      961 acaactaatg tttcttgtaa agaatcttta tatactttag ctgtttctaa ttttagagat
     1021 agttctgtat ctccttctaa tggtgaataa tcatacgtat cgccatcatt tgctgaatcg
     1081 atcaaatgaa cgaaatcaag atattgtcta tctccaacta tcaaattcaa ttgcccatct
     1141 ttaaaagaaa gtttatattt attgttactt atctcagttt tgtctatctc gattgctgtt
     1201 tctaattttt cattattttt ctcaaattca acaacagtgt atcctagtgc tggcacattt
     1261 acatttattt ttatgtcaag ttcataataa gctggttcat caaaatattc gaaacctgta
     1321 gcaatcatcc gataagcatt tttccgttct ggaaaataaa cttctttttc aatgatatta
     1381 tagggactac ctttgaagtg tattttcttt gaatcagcta ctacatgcac gattttcgat
     1441 ccactataat tctcagtcgt tgtgttgaaa acaaggactt cattcgattt tagctttaat
     1501 gcatcagcca tccgttttac aataaggttt tcgattccgt cagctatctc attagcttct
     1561 ttgattcgat ggaaaatatc ttccgcaaca ttatctgaaa cacagccacc aatactatca
     1621 tgagcctggt tttgcaaaat ctttttccat aaccgacgaa gtaggccttt actgatatgg
     1681 ataccatttc tttctgcaat cagcatgagc ggctcaactc gttttagaat tttttgttcc
     1741 aatttaaaat tttctatttt catttttgtt cttactgagc caatagaacg atgaacacga
     1801 gagtaaaccg gctgacgaag ttcaccttta tattcaggta attctttttt gtttctgata
     1861 acttctacat aagaaggata atctgaaata atattgtgga atttactgta tttattcaac
     1921 ttctctacga cttgatcaaa atttaaaatc attgactttt gatctacacc cgaaggtact
     1981 aaaatatctt tgttttttcc ttgcatatga agcatttcaa ctgcttcatc caacttttct
     2041 tttgcaaatt caggaatatc tttaattgcg tctaaggtca tcattccagt catataacca
     2101 aatggaaaat tcatggcata aacaaattta tctcccaatc ctttccagat aaaataagga
     2161 gatggaacca tcgtttcaaa gtttaatcct cgccaacaca tgaaattatc gatttctact
     2221 tgatgcaaaa gtgtaggcaa attcgcattg aacccaaacg tgtcaggcaa ataccctacc
     2281 atcattggat ctccatattt aaggatcgta tcactgattc caatcattaa gtttcttaaa
     2341 attgattcag catccactaa taaggcatct gtttgtgcat accacggtcc cacgaatagt
     2401 tgtcctgttt tcatcagtcg ttggataacc ttaagctttt gcggattgat ctccaaatac
     2461 tcatcgacga ttgaagtttg tccatctaaa caaaagttta ctttagggtt cctttccaat
     2521 tcatctagaa cttcagtaaa gacctgatca gacaatacga tggcatcttc atttgtaaaa
     2581 taccattctc tgtcccagtg cgtgtgttga attatatgtg catttaccat atcagattct
     2641 cctactttcc ttcttctact gaatttactt gttgttcttt tttatacttg atggatttta
     2701 gtgcattgac cattaacgct gtaaccactc ccccaataac aattgatact aaatataaag
     2761 gtatattcgt aacaaaagga attgccataa aggttgaaag cattgtttct tgagtaacac
     2821 caaaaaatcc atttagacca catgctactg cagtgcccac cacgatgctt ggtattactc
     2881 gtaaaggatc tttcacggca aacgggatcg tcccttcagt aatccccaca aggcccataa
     2941 tcatagctgt ctttgcatta tctcgttccg attcatcata aagttttttg tttaaaattg
     3001 ttgctaaaaa cattccaaat ggcggaatag caattcctaa taacatggct gtactaggtc
     3061 caaagcttcc gcttgaaaaa gaagcaaccc caaaagcata tgcgatttta ttgataggtc
     3121 ctcccatatc aaacgccatc attcctccta aaatagccga catcaaagca gcatttgctc
     3181 ctgacatgcc ttctagccaa ttagtcagtc ctgttgtcat tgcttgaata ggcgtagcta
     3241 gtacatacca taatatgatt ccaacaacta atgtagtaac aaaaggaatc agcataattg
     3301 attttagact aataacttgc atcggtactt ttactttttt caatgcatta gctaaatagc
     3361 cacaagctaa tccagcaacc attcctccaa taaatcccat accaagattt tgacacatca
     3421 tcccagtgat aaaagctggt gctagaccgg gacggtcagc aattgaatat gcgatatagg
     3481 ctgaaatcat cggtacgata aactgtaatc ccgtctgacc aatcattcgc aggactcccc
     3541 agaatgtatc cgtctggtcc caaacacctg tgccaccaaa aatgactgca atagaagtta
     3601 gtaaccctgc tgctacgaca agcgggatca tataggaaac cccactttgg aaatgattta
     3661 gaatttctct tccttgtttc ttgaattcca ttagtaactc ccccacgata tattttttat
     3721 ttgttaatta atgcaattgc ttcatcaatc gtctcttcac catcttttac tacctctgag
     3781 gtccctactg ttaaaatcgg tttaccgtca aagcgacctc gttctgaaac atttgtatct
     3841 actgcaaata tgacaacatc tgcctcggaa acatccgaaa ttgaaagttt gttttctatc
     3901 cccatagccc cctgtgtttc tactttaata tctaaccctt tcttcttcgc aaacttttct
     3961 aaattagctt ttgccatata agtatgggca atccctgcga tacatgctgt tactgctact
     4021 atttttgtca tatgaaattc ctccttttaa aatgatagag cttttaccaa ttctttttta
     4081 tttctcgaat cttttattct tttaacaaac tcgtcatcca tcaatcttcc agataattct
     4141 gctaaaatac gtaaatgatt agtaccttta tcagtgtctt ttattgctaa caaaaagacg
     4201 agatcaactg gttgatcatc taatgcgccc cattcaacag accgctttaa cttggctaca
     4261 gccactgttg attcaattac tgctaggctt ttgccatgag gaatggcgat atttttccca
     4321 attcctgtgg tcatatggtt ttctctgtca aatacgtctt tttgaaattg tttgtggtca
     4381 gacacaaacc cattagtctc taacaatttc gataattttt cgactacact ttttttatca
     4441 gatgcattta aatctatcac tatattattt atattgattt tttcactgat catttataag
     4501 ccttctttca atatataaac taatttttca aacgacgaac tcgtttttaa atttgctaat
     4561 aactcatgat tcgaagcgat ttcattgaat gtttcataaa ttttattcaa ttcaggtttt
     4621 actttttcat taagtgcaaa aaagaaaaca gaatgaacaa gtgtcccatc ccaatcaatt
     4681 ccttctggat taacgtagac acagattact ggctttttta taaaatctac ttcgatatgc
     4741 ggcatagcaa aatcattcat cgctgttgaa gatagttttt ctcttttgat tgctccctct
     4801 agtatccctt ctttcgcgta attttctttt accaattttt tggtaattat atttaaaacg
     4861 ttcacttgat tatcctttat ttggtgggta aaaaagcaaa atcgtttatc taataaattt
     4921 agaaaagcat cttctgtttg ttcatttaac tgtagatact gttttacttt tctgacatct
     4981 gcccctaaca tcaaaggact aacaacaata acaggaaaat ttgtattttc aatcggaatc
     5041 gtgcttataa caagttcatc agtgactttt ctatcttgta gttcattgat gcttagtaca
     5101 tcttctatct caaccatatc tgcaaaaccg ttatttattc tttgctctaa taattttgat
     5161 gttccatatc cgctactaca aactaatatc actttcgttt tgtcaggttt gtaacgatcc
     5221 aaaagtgatt gaatatggag tgttacgtaa gcgatttcat cttcattaaa atgtatacag
     5281 aatctctctt cgatttcctc taataaatct acacttatca taaatgacct tttaaaagaa
     5341 tatttgattt tgtccgtata agggttatag atatttactc ccaatttgag tcttttaact
     5401 gctgcattga gatgtaatac tagattattg atcaattctt catcaggata aaattcattt
     5461 aatttaattc ttactaactc ttgtagctca acacccatat ctaaatagga aagcttcgtt
     5521 tgatctaagt cgtgattcat agcatttttt tcaatcgaag taatgtgaat gtcaacataa
     5581 gcttgttcat ctattggcac aataattgaa aatgcttttt caatcatttt gattaaaata
     5641 agtgtattct ttgagatttc ttcttcagaa ttattactac gtgataagaa attgcttgtt
     5701 tcaatccttt ctatcgcaat agcaaggtgg atagctaaag tttgaaattc ataatctgta
     5761 aatttcaatt gtgtcttatt catgtatcga ttaagcaatt cggctacttt tttcaatatg
     5821 tccttattta agaaattatt cgcttcacca atgaaattga tattcaagtc taagagatct
     5881 gttttactgt tagatgctgt aaagccaccc caataatagt tcacaacttc tgacatcaac
     5941 cttcttttac ttttttcatc agctttgatg cgcacgccat ttcgatctga aactaaggac
     6001 atcccatgct tgtttagtct ttttctaaca tctttcattg tgctttctac cgtcgacctg
     6061 ctcacgtata attcgtcagc aagattttga atctttagag aatcattagt attcaataat
     6121 ttagcaaaca cataagttag acggtcctcc ggagagtctg cgatattgat tttatgactg
     6181 gataataaat ttcgaagtgt ttcttttttt ccaataagcg aaacgccttt tttaggttga
     6241 accaacattt gtacatctgg cattttttgg attatttctt tcaaaattaa aatatctttg
     6301 ctaattgttt tcgtagatac atataattgt tctgataatt ctttgtaagt tgtgtgctca
     6361 ttttcaatca aaaagtatag tattttcatt tgccgttcgc taaattcgat gcccattatc
     6421 ttcacctcct gaatacagct taagtgtaca attattatct ctattttcat acagaagaaa
     6481 gttcttgatt aatgtagaag tttctaatat tcttgattat ttcaaatcaa taaaaaagcc
     6541 tgcaatattt atatcacagg cttttttctc tattaaatat atataagata atacattttt
     6601 ctactagttc tcactaatct tgtataatta aaccctccgc aacttgacgg agatggagaa
     6661 gatgcccccg tctaatctag cggtcatctc tcctcccatt ccttccacca atttttttga
     6721 caggtaaagc ccgattccgc tagattccgt atttgtgcgg ctggtatttt cggtataaaa
     6781 acgctggaag actttttcaa cagaggtttg cgttcctggt ttgataggat tggccactgt
     6841 taatcgaatg aatggatcct cttttgaaaa tgtcaccttt gcttgtccat ctgcataacg
     6901 caataaattc tgtatgatat tttgcagcac tcgcagcaac atgagcggat ctgctaatac
     6961 agttgtattt atcgagtgtt cagcgaacgc tacctcgata tttttctcag aaaattgttc
     7021 ataaaaagag aaaaatactt cttccacctc tttagaaaaa gaaagcgtat ggagatccat
     7081 ttctttttgc ttcgtctcta ttcgagcaag atcataaaac tgatccgtca tactgagtaa
     7141 tcttgcaaca gattgtttga tgatcgttaa atacttctcc cgtttctccg tatcctctac
     7201 ctgttccagt aattgtacat aaccgttaat agcagtcaaa ggagttctga gatcatgaga
     7261 aaggccgacg atcgatagtc tgatgttttc ttccatttct tgaaatgctt gtcctttttg
     7321 ttcattttca tccaaccatg tattcagtgc atctactagg tccattagat tcttttctcg
     7381 aaattcgact gaaagacgac caccatattc ttgtcgttca gaaagagtgg cgattttctt
     7441 agttaatctc ttgatttccc tttgcaggaa gaaaataaaa cttcccataa agataacaac
     7501 gagaagcaac agccatttaa tcaaatctaa accccactcc ccacaccgta tgaatataca
     7561 cagcatgtgc ctttgctagc ttccctcgaa gattggaaat atgcatattc actgttttct
     7621 cactatcaaa atatggttct tgccaaactg tttcataaag atttgctcgc gaaaatactt
     7681 ttttcggatg ttctaaaaaa agcttcagca gatcgaattc tcttcctgtc aaatggacag
     7741 ccacaccatt cacatttaca tccctcgact ctttgtccag taagatttcc ttgtaataga
     7801 tgcgttgcga cagatctttt acttggacag aagtctgatg acgaagttgg atgccgattc
     7861 gtaacaaaag ttcttcgata tcaaatggtt tcgtcacata gtcatccgct cctgctgcca
     7921 acaaggaaac tttatctctt tgatcgcttt ttgctgaaag aacgatgatc ggaacattcg
     7981 atgtctgtcg gatttctgct aacaatgctt cacctgaaag ccctggaagc atgagatcta
     8041 gaaggatcaa atcgacagga tatgtgctta acaataattt tccttccgta cctgaataag
     8101 catcaagaag tttgtatctc tgttcaagaa cctcctccag taaggaatga acagcttcat
     8161 cgtcttcgat aatcaaaata gtttccagca ggaccaccta cttaatgtca gatttttgga
     8221 aaataaacat tcctagagct gatgatacca cgattgttac tagactgacc agaatcattt
     8281 tcggtgcttg cgaataagtt gcttgttgcg caaagtccgt atacatgagc cacgtttccg
     8341 taaaccactc cgttagaaat tccaagcctt tgatttttgc gaaaacaccg ggaatgaagg
     8401 ccgttcccat gatataaaga aaagaaagaa gcaaactgac actggtactt ctagtgatga
     8461 atgtcaaagc cactggaaca gataatgtcc ctaatgtcag cagaagcaat acaggaaata
     8521 cttttagaac ataatttcca tagtttttga ttccctcaac ttcaccaaat ccgaaagcaa
     8581 gtgtgtaagc aagcatggct agtcccgtga tcaaaaccac gccaagtgtt gtgatcaaaa
     8641 gcgtaacgag aagtttcgac acataataag tcattcgatt ccgtcctaaa gataacgtat
     8701 tgcgaatcgt accgttttga aattcttccc caatataaaa gcacgcaaat gctgcgaaag
     8761 ccagaagaat ccatgcagcc attttttcta ccattaaaat ccctgttgca ccgttgatga
     8821 ctgtatccgt tgcttggttt aattcattgc cgtattttgt atgcatatac cattcgttcc
     8881 aagaataaaa aagcggaagt acgaccatca gtcctagtga gacccacatt ttacggctct
     8941 tccatacttt tattttttca gcagttaata gattaaacat gtggattgcc tccagttaat
     9001 tgtaagaaat aatcttccag tttttgccca actaggtgaa taccatccac aggaactttt
     9061 gcttctacca atgcttgatt gatggctgct acttggtcaa ttccttcaaa aagctggata
     9121 acacggctag attgtacaaa ataatcccta taaccaagag agtcgagaac cgttactgct
     9181 tgttccgtcg ccgaagtgtc aatttttata aattgacgag attcttgtgc caattcttct
     9241 ttcgataatt ctttgatcaa gtgtccttca tgcaaaatac catagtgagt ggcgatctgt
     9301 gacaattcat ctagcaagtg gctagaaagc aagacggtga tgccccgttc ttttaccaaa
     9361 cgttggatga tttctcgcat ttctgccatc cctgctggat ccatcccatt cgtcggttca
     9421 tccaaaatca aaaattctgg attcgtgatc agtgcatttg caatagctaa acgctggcgc
     9481 atacctaaag agaaattctt tgctttcttt ttccctgtat tttccaatcg catcattttc
     9541 agcaggtctt cgatttctct atccgacaca ccgccgttcg ctgcttggat acgtaggtta
     9601 tctcttgcag ttaattctgg ataaagagca ggggtttcaa tcgattgacc catacgtcgt
     9661 cttgctgcct gcaaatccgt cgttccaaat aattcgatcg aaccttcatc aatcgagatc
     9721 aaacccataa tagttctcat aaaggtagac ttacccgctc cattttcccc aatcaatcca
     9781 tagatcattc cacgtttgat ttcgattgat actttatcta atgctttggc agcaccgtat
     9841 tttttggtta taccagttgc tttcaagacc gtttcatttc caaaatccat acttatcctt
     9901 ctttcttatt gatgagtgaa gtatagttga cgggtataaa gaaagcagcc agaaatgtat
     9961 aaagaaagtg taaattttcc aattgaatgt caaaaaaaac aatcggacaa tacgtgtctt
    10021 tcctattatc aggcagtttg ttatacgctt gtatcagttt cctgattctt tcttttcgac
    10081 caaagtcgtt tctctgtatt cctcttcact gaaaattttt gcttctttca gatcaagctt
    10141 gagtaatttt accagaccgg ttataaggat aactatagca agactccctc ccagaaaaat
    10201 tagactgtct cgcagacgtg gttgcgttgt cttccaatca aagagcgcac caaacagtgc
    10261 taatccaagt ggactggcca ccatggtaat cgttgttatc gttgacataa gtcgtccttg
    10321 tatatgcgtt ggaacttctc gctgaagcca gaccatataa aaagcaaaag aaaacattaa
    10381 tccttaaaaa cggacttatg aaaaataata catgccgttt gggatttaca attagctacc
    10441 aaataagagg ttatgacaaa agacttttca caatcccttc ttccgaataa acggtgttct
    10501 cctgccaact cctcggtcac aagtcacaat atttatcaaa catgagaagc aatttttgta
    10561 aattccttct tatttcttga agctgaccac ttctgtcaca acctcagaag cggataaata
    10621 atattgtttg atgatacttt atctttttcc cgtttgttta tagatcgaat gatattatag
    10681 accaagaatc ctacctatta acatagctaa aaacacaaaa aaggcacttt ccaaattgtg
    10741 gaaagtgcca gaaaagttag gattaaggta actgcgctca gcattgcttc gcccagtacg
    10801 attctcgtaa ctcgtgaact cgaacgataa tcgcaattaa cgttttgaga actgtgaagc
    10861 tttacgggct ttcttaagac ctggtttttt cttcgtatca ttgaataagt ttgaaaaacc
    10921 ttgatttata aaggttttcc cgtaaatata ttttattata tttgattaaa tttgaaatgt
    10981 ttgtgttcta aaatgtgttc taatttaaag gcagtatatt tcgtatttta gattaaattt
    11041 ttaggctcat tcattctcaa atccattcta ttcagtcaat aaaaatttta aaaagacttt
    11101 ataaacaaag atgttcaagc gagcatctta tttttttgtg cttaatttga aaggagaaca
    11161 tttcatgtat aaaaaacaaa aaaagaaatt atgtgtatta actattttga gtttggctgt
    11221 tcaagcaatt gttggagcaa ttgctcctac gattactttt gctgatgaaa ttacccatcc
    11281 acaaacggta accgtagaac tagatttagc acatcaatat gctgttgaag gaacttttag
    11341 tgatggtcgt ccaatgtcag aagtcactgt tccgcattat gcagtgtaca acggctttaa
    11401 acaagatatt ttctgtattg aaccaggagt cccgatttac aatgagttca ctcctggcta
    11461 tgagaaaaac ccgttaccag atatgtccga taaagccaaa ttagtttccg ttttatggaa
    11521 aaaagtaggt acagatgcag atactcatat tgtggcacag aagatgatct ggcaagaagt
    11581 taatgggtat acccttcact caataaaacg atcagatggt agtgccgtaa atatcgcagc
    11641 cattgaggca aaaataaatc aagccattga cgattatcag aagaaaccaa ccttccataa
    11701 tagtacggct aaaacagtat tagggcaatc gactactgtg acggatacga ataatctgaa
    11761 tttgtcagag ttcgatgagg ttgtggaaaa tgccgcaaac attgattatc gtgtgaatgg
    11821 taatcagttg ataatcaccc caaatgccaa ttcaaaagaa agtggtgtgc tgacgcttaa
    11881 gaaatctgct ggtaccggaa cacctgtcgc ctataaaaag gctggccaac agactttaat
    11941 ggccggggcg attgataaac ctaataccta cacaatcaaa attgatgtgg aaacggaggg
    12001 ttctttaaag atcaaaaaag tcgataaaga atcgggtgat gttgtaccag gaacggtttt
    12061 ccatttagac tttggaaaaa ctttacctgc aaaagacgtg actactgaca aagaaggcat
    12121 tgctacattg gatgagattc ctcatggtac aaaagtaacc attaccgaaa aatcagtacc
    12181 ggcgccttat acgattgata ccacccccat gactaccacc attaaagcag gcgaaaccat
    12241 ttacgtgact tcaaaaaacg cacgggaaaa agggcaaatc attctggata aaagcggagt
    12301 tgaaacaggg agtgatcttt ggaatgacaa ttattcctta gctggtaata cgtttgccat
    12361 tcgcaaagac agtccaaccg gtgaaatcgt tcaagaaatg accacggatg aaaacggtca
    12421 tgcggaaaca ccaaaagaga ttgctaatgc gttggaattg ggaacctatt atgtgaccga
    12481 aactaaagct agtcatgggt tcgtgaatac gttcaaacct gtaaaagtcg aattgaagta
    12541 tgccaatcaa accgtagccc ctgttaccag taacgtaaaa gggcaaaatc aagaagtcac
    12601 tggcgaaacc actttgacga aagaagacaa agatactggg gataaagcac aaggtaaggc
    12661 agtctttgag ggtgctgaat acaccttgtt tactgctaag gatggaaaag ctgttaaatg
    12721 gagtgaggct tttaaaccag agttagcgaa gggaacgaaa ggttctgatg aaacggtcac
    12781 tttgacttta gacgaaaaga accaagttgc cgttaaacat ttagctatca acgagtatta
    12841 ttggcaagaa acaaaagcgc ccgaaggata tagccttgat gaaacaaagt atcctgtatc
    12901 catcaaaaag gtggatgata gcgaaaaaaa tgccgtgatt acccgaaatg ttacggcaaa
    12961 agaacaagtg attcgctttg gctttgattt ctttaaattt gctggatcag ctgcgggcac
    13021 taccgaaact ggcttcaatg atctgacctt taaagtgtcg ccattggaag ggaccaatga
    13081 aatcacaggt gccgaagatg aagcgaccac agcttataac gagcaattag ggtttgatgg
    13141 ttatggcaag tttgaaaatc ttccttatgg ggattattta cttgaagaag tagaagcccc
    13201 agaaggtttt caaaagatta cgccattaga aatccgttct acttttaagg aaaacaaaga
    13261 agactttgtg aagagtgagt atgtctttac cattaccgaa caagaccaaa aacaaccaat
    13321 taaaacggtg accgttcctt acgagaagct gacgaacaaa gcattttctg ttagtttgaa
    13381 ccgtttaatg ctctatgatt tgcctgagga agaagatagt ttaacttctc ttgcgacttg
    13441 gaaagacgga aacaaagaat taacttctct ggattctacc gagctagttg ataaattgag
    13501 ttacaatcta catgaaatca aagaagactg gtatgtggta gcgcaagcca ttgatgtgga
    13561 tgcgacaaaa gctgcccaag aaaaagatga aaaagccaaa ccagtggtga ttgcagaaac
    13621 aagcgcaacg ttggcgaaca aagagaaaac aggaacttgg aaaattcagc ataaattaac
    13681 cgccgaacaa gttttaaata aaactattgt cttattcaac tatgtgtatg aaaataagga
    13741 agcttttgaa gcaggcgata agccagtagc gaaggatgtt agcttgaata accaagccca
    13801 aactgtcagt tgtacagtgg aacaccatgt ttctatccaa acaaaggccc acctagaaaa
    13861 cggttctcag acctttactc atggtgatgt ggtggatatg tttgatgatg tgtcgattac
    13921 tcatgatgta ctggatggct caaaagaagc tttcgaaaca attctgtatg ctttattacc
    13981 ggatggtaca aacaaagaaa tttggaaatc tggcaagatt gattatgaag taaatgataa
    14041 agaattcacc aaaactgtgc ttgcgaaaaa agtagatacc ggaaaatatc cagaaggaac
    14101 caagtttact tttgcggaaa tcaattacga taaagatggg aatatcaatg gaaaacacaa
    14161 tgaagatttg aaagaaaaat ctcaaacctt aacaccaaaa gaagttccaa ccatactaag
    14221 tacgccgaaa caaccggaaa caccaactgt tccaagtggc tctcaagaat ctagtcccac
    14281 agtgaagacc ttccctcaaa ccggggagaa aaattccaat gttttactgt tcattggttt
    14341 tactttgatt tttgcgacgg cgggctatta tttctggaat cgccggaact aaggtgatga
    14401 gatgatacaa gtaaatccta agaaccaacg tgcgcgaccg cctcctaaaa aagtagtcgc
    14461 gctatttttg tatccaaaac taacaatgaa aagaggaaaa gaagatggaa ttgaaatttg
    14521 tcgtgccaga tatggctgaa acctttggaa agttaagtta tgccggtgaa ggagaaatta
    14581 taacagaagg atatggacgg aatactacgg tgattggtcg cagttatcat ttgtattcca
    14641 gtaagcaacg ggccgatgat attgaggtgg tagtagctgc ggaagccggt gaaaaggatt
    14701 ttgatcaaga ccaaccgcta aaagccgtga atccccattt ggttgccaaa ggctatgaga
    14761 ttgagaatcg tgggtttact gattacgtgt tgtatgttga tgatttagtg aaagcatagg
    14821 aggaaaagaa gatgagatta acagaaggta ttgtcgtaga ttcaggttta acatttggga
    14881 agttgcgttt ttccgcatta cgccgagaag tacggaagca aaatgaggat ggaacaattg
    14941 gtaatgaggt aaaagaacga acctataatt taaaatcctc tgcacaaggg cgaatgattc
    15001 aagttagtat cccagctaat gtacccttac gcgagtttgc ttatgatgca gaggtagagt
    15061 tggtcaatcc aatcgtggat accgttgcca attatgtttt tcgagaagga actaccgtta
    15121 attggttcat taaggcggat gatttagtct tgaagcggca gccgaatcaa ggaaatccta
    15181 cgaaccaaaa tgaaagaaag aaataggtga agattatgga aattaatgtt gaacatattt
    15241 tagattgtct cgatcaatat ggaaaaggcg agctgactga ggaacaactc gttaaagcat
    15301 taacctctga tgaaaaaatg tttctgatta tacatcaagg tttacttgga gaaaatagcg
    15361 acagtgaaga agactctgat gtactagact ggttaggaga agaagagtct ttctttatag
    15421 ttgtcgaagt aaatgaaaac ttatgtcggc aggcagaatc cattttggaa gaaattggtg
    15481 tggagatgcc ggatgctatt gaaggctttt taaatcaatt ggttgaaacg aaacaactcc
    15541 cctcctcgta gcagtaaatg actagcataa ctttttgaaa ttgcctaatg aaatcctatt
    15601 gggctttttc gattggaggt ggaaaccatc aaaatgtaca aaggacatcg catacgcgca
    15661 ggagatcagc acttggttta tcactttgtt cttggctggc tactcgctct gttcatcggt
    15721 tggatgagcg tcttttattt tcaagagttt agacaatttg atatttctaa gctatcactt
    15781 tctactattg aaatcgtttg gtctataaaa gatttagtat gtctgcttgg aagtctcgct
    15841 ttttcaggag ctatgatatt gctttatatt cacttttttc ttgatcattg gcgaagcttg
    15901 tggcatcgac aaaagctagc tcggatgatt ctagagaacc attggtatga agtgaaacaa
    15961 actcaaagtg aaggcttttt caaagatttg aatagtagcc gaaccagaga gactatcagt
    16021 tacttcccca aaatctgtta tcgaatgaaa gatggattac tgtctattcg cgttcaaatt
    16081 tcacttggga aataccaaga tcagctttta aaattggaaa agaagttaga aagcggactg
    16141 tattgtgagt tagtagaaaa ggaactcaaa gactcctatg tggagtatac tttactgtat
    16201 gatatgatcg ccaatcgaat tgggatagac gaagtagtgg cagaaaatgg gactctgcga
    16261 ttgatgaaaa atcaagtatg ggcttatgat tctttacccc atatgttaat cgctggtggc
    16321 acgggtggag ggaagaccta tttcctcctc accattatcg aagcactatt gaagtcagat
    16381 gcagaattgt ttatcctcga tcccaaaaat gcagatttag ctgatttagg tacggtgatg
    16441 cctcatgttt attcacaaaa ggaagaaatt tctgcttgtg tggaagattt ctatgaacgt
    16501 atgatggctc gtagcaaggc aatgaaagaa atgtctaact acaaaacagg agagaattat
    16561 gcctatcttg gacttccacc caacttttta atctttgatg aatacgtggc ttatatggag
    16621 atgttaacga cgaaagaaag tgcggtgatt ttgaataagc tcaaacaaat cgtgatgttg
    16681 ggtcgtcaat ctggtttctt tctgattttg gcttgccaaa ggccggatgc gaaatattta
    16741 ggagacggaa tccgcgatca attcaacttt cgcgtggctt tgggtcgtat gagtgaactc
    16801 ggatattcca tgatgtttgg ggaggtcgaa aaaaacttct ttatgaagca catcaaaggt
    16861 cgaggttatg tggatacagg tggcagtgtg attagtgagt tttatacgcc acttgtacca
    16921 aaagggtatg atttcttaga ttcaattagt aaattagaaa agatgaatga ttgagtgatc
    16981 ttcaatactg actttatact tcaagtaacg tataatactt ttatatagta ggaagaaagg
    17041 ggataattaa tatggaaagt aatgattctt atttagagta tgcaaaagca aataaggata
    17101 agtttatttc ttatattatc aaagggaaat cccccgaagt tggacgagac gcagttttta
    17161 tggcagggag tcctggggct ggaaaatcag aggttgccat tggattagct gagaattatg
    17221 ataaccatgt gattattgat gcggattact ttcgcaccca gtttcctgat tataatggaa
    17281 aaaactctag tctctttcaa aaagcatctt cttggttggt agagcaggca tttagatatg
    17341 tcttagatca tggctattct tttattctgg atgcaacttt tgcaatatta agtgctgaaa
    17401 aaaatatctc gcgggcgttg aaaaaacatt ttcgagtgac gattttctat gtttatcaag
    17461 accctaaaat cgcttggaaa tttacacaag aacgtgaaat tgcagaggga cgtcatgtgc
    17521 cgaaaaaggc atttattaac gcttttttca aatcaagaga gaacattgca aaagtaaaga
    17581 gtaaacatcc agatgtattg cttcatatca taataaaaga ttatacgaat aatatttccg
    17641 aagttcatta tgctacggat aatctagaat tatttttacc actagggtat agtaggaaag
    17701 atttggaggt tcaattacat gactgagaaa gaacaagtaa cgaaaattgt aaagaaatac
    17761 aataagagca ttgcggattt atcggaaaat gctactgcaa aagagttcaa aacggtgatt
    17821 aaatatgtag ccgatcaggc caatgaaaaa caacggaaat tagttggatt aaataaaaaa
    17881 tagtaggaca aattaataat gtatgagcca agagcaaaat gttcttggct tttttctttg
    17941 caatgaatga ggtaatgtat gagattttta gatttattcg ccggtattgg tggctttcgt
    18001 ttaggtatgg aacaggcgag ccatcattgt atcggttttt gtgaaattga taaatttgct
    18061 agacgtagtt ataaagcaat tcatgataca agtaaggagg tggaaatgca tgacatcaca
    18121 agtgtatcag atgagtttat tcaatccctc ggaccagtgg acatcctttg cggcggattt
    18181 ccgtgccaag ctttttcaat tgcgggaaag cggcaaagat tttccgatac tcgaggtact
    18241 ttattctttg aaatcgctcg gttcaccact cttctccgac ctaagctttt attccttgag
    18301 aacgtccgag gattgctcaa tcacgaagga ggggctacgt tcgagacgat cctccgaacg
    18361 ctggatggat tggggtatga cgtggagtgg taggtgctta actcaaaggc ctacattccc
    18421 caaagccggg agcggatatt ccttatcgga cattctcgag acgcatgtac cgaacaagta
    18481 tttcctatca ttggaccgtc ttcaacatct gatcaaaaca tcagaaattt ggtaaatatc
    18541 aatccttcca atcgaggaat gggtggacaa gtctatggtt cagatggcgt agcgccaacc
    18601 ttaacaggtg atgaaggaat caaaatcgca ctgccagcaa gggatggaat ctctgtagca
    18661 ggactattac cgggaaagtt tgaacaaggt aatcgagttt atgaagttac tggaaccgcc
    18721 cccaccttgt caacgaaaca aggtggtaca aaaatcatga ttcgaaaaaa tgaaacagaa
    18781 tatcaagagg tgaagccaga agacagtgtg aatctggctt ttcctaattc cacaagtcgc
    18841 cgaggtcgtc tgggaaaaca gagtgttcat accttactaa caggagacca acaagcgatc
    18901 gtcacggatc agtatcaaat tcggaagcta acacctagag agtgttggcg acttcagggc
    18961 tttcccgatt gggcttttga tcgagattct caagtcaatt cagatagtca gttgtataag
    19021 caagccggaa actctgtaac agttccggtc atttttgata ttgccagaag attaaaagag
    19081 ttggatgtac atggtttatg atgagttgat tggagaaatt tattgggtga ttggaaagat
    19141 tcagtcagac ccagaattag aagaacaatt gcgacggtta aaatttgata ttcgcaagaa
    19201 tggtgtcaaa gtcgctggcg atccatattt aatgaatgag gagacggatg ctcgaattga
    19261 aataaatcaa gtaattgcag aatttgagcg gattgcggac caagcaaaag aaccagatat
    19321 tcgtcaatac ttgttagaaa tgaaagcaga gttggaagta aatggtatta cagatgagta
    19381 acctaaacca acacaactga ctgttaggcg ctgaccgcgg aggattcgcg aagcagaaat
    19441 cctccgtggt gctgtgcctg ttcatggacg gcgttagccg accatgatgt ctcacccccg
    19501 tatctaacag gggggtacaa aaacacaaaa aataagtaag caaccattaa gaatgcttgt
    19561 tgtatcaaag atagtaagta atttgtatag gtgttaactc atttgaaaaa gttaacacct
    19621 tgaatttgga ggtgagaact tggcacaacg gaatttagat tatcggctat taaaagaccg
    19681 acgcaacgaa tacggcgttt cacaaaataa attggctacg gcttgtggac ttagtcggcc
    19741 gtacttaaat cagatagaaa atggtggcgt aaccgcatct ataaaaacca tgaggaaaat
    19801 ttttgaccaa ctggaaagct ttaatcccga tttgcctttg acgttactgt ttgactatgt
    19861 aaggattcgc tttcccacaa cggatgcacg aaaaattatt caagaaattc tccatttgaa
    19921 atttgagtat atgctccatg aagattatgc cttttactcc tatcaagaac agtatgtcat
    19981 gggtgatatt gtggtgatgt tgtctcatga agaagataaa ggtgtgcttt tagaattaaa
    20041 ggggcgaggt tgccgacaat ttgaaacttt tttactcgct caaaagcgta gctggtacga
    20101 ctttttcgag gattgtctaa aggccggtgg cgtgatgaaa cgcttggact tggcaatcaa
    20161 tgatcgagtg gggctattgg atatacctga gctaacgaaa aaatgtcaga aggaagaatg
    20221 tatctccttg tttcgtacct ttaaaagtta tcgttccggg gaacttttaa aggctgatga
    20281 aaaggacggc atgggcaata ccttatatat cggcagtctc aagagtgagg tttatttctg
    20341 tttgtatgaa aaagactatg aacaatatat caagttaggg attccattag accaaactga
    20401 gacgaaaaat cgttttgaga ttcgactgaa aaatgaccgt gcttttcatg cgattcaaga
    20461 tttgttaaaa ggtcgtagca ttgagagcac cacgttttcc attatcaatc gttacttgcg
    20521 atttgcggat aaagtagaag gtaaaaggcg aaccaactgg cccttgaatg aacaatgggg
    20581 gcgttttatt ggacggaatc ggaaagagat tcaattaact tccgagccaa aaccgtatac
    20641 cattgaacga accttgaatt ggctaggccg acaagtcgcc cctacgtgga aaatggctaa
    20701 ggagttggat cgattgaatc ggacaaccta tatccaggat atggtacaga atgcacgctt
    20761 atcagaccga cataaaaaga ttttggagca acaaagtatg gcaattgaaa atttgattat
    20821 atgaaaagag gaatcgtcat gggaattgta aaagatattt ttttggtatt tggtggcagt
    20881 atgcttggtg ttactgtgat gtgcttgatg aatgcaagtg cagcagcgga tcgagcaatg
    20941 gaaaagaaag gaaagtgaca agatgaattt cggacaaaat tt
//
LOCUS       DACDXO010000049        19821 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E23605_2017
            SAMN12639989-rid9881463.denovo.050, whole genome shotgun sequence.
ACCESSION   DACDXO010000049 DACDXO010000000
VERSION     DACDXO010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639989
            Sequence Read Archive: SRR10023389
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741914.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:42:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,972
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 33 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 17 of 73
            Pseudo Genes (multiple problems)  :: 10 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19821
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639989-rid9881463.denovo.050"
                     /isolate="E23605_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            6..305
                     /locus_tag="IWM35_001961"
     CDS             6..305
                     /locus_tag="IWM35_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP9919806.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            378..1739
                     /locus_tag="IWM35_001962"
     CDS             378..1739
                     /locus_tag="IWM35_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HAP9919807.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFMSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLPFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGIITMPIYLYGATYLAPILSDLAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            1761..2021
                     /locus_tag="IWM35_001963"
     CDS             1761..2021
                     /locus_tag="IWM35_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9919808.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2035..2787
                     /locus_tag="IWM35_001964"
     CDS             2035..2787
                     /locus_tag="IWM35_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HAP9919809.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(2881..3486)
                     /locus_tag="IWM35_001965"
     CDS             complement(2881..3486)
                     /locus_tag="IWM35_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP9919810.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(3539..4513)
                     /locus_tag="IWM35_001966"
     CDS             complement(3539..4513)
                     /locus_tag="IWM35_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HAP9919811.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(4891..5670)
                     /locus_tag="IWM35_001967"
     CDS             complement(4891..5670)
                     /locus_tag="IWM35_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP9919812.1"
                     /translation="MLLRVVGSIIFRYTLEEVNNVPKYEEIANILRERIRSKEYPLDS
                     FLPNQVDLVKEFGASRMTIKKAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEY
                     EGLSLQMKRRHRSLKSQIIHFDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVL
                     EHTFMPVHLVPGLKKEHLLASIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAE
                     NDPVLEVQQVVYLKDGQPIEYSSSRNRFDVRNYSVLDVRST"
     gene            complement(5737..7008)
                     /gene="celB"
                     /locus_tag="IWM35_001968"
     CDS             complement(5737..7008)
                     /gene="celB"
                     /locus_tag="IWM35_001968"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HAP9919813.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWVVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7161..8558
                     /locus_tag="IWM35_001969"
     CDS             7161..8558
                     /locus_tag="IWM35_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP9919814.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            8639..9079
                     /locus_tag="IWM35_001970"
     CDS             8639..9079
                     /locus_tag="IWM35_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAP9919815.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            9227..9553
                     /locus_tag="IWM35_001971"
     CDS             9227..9553
                     /locus_tag="IWM35_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAP9919816.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            9554..10303
                     /locus_tag="IWM35_001972"
     CDS             9554..10303
                     /locus_tag="IWM35_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9919817.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
     gene            complement(10383..10838)
                     /locus_tag="IWM35_001973"
     CDS             complement(10383..10838)
                     /locus_tag="IWM35_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAP9919818.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNLENSTDLDDEFLNYYI"
     gene            complement(11265..11540)
                     /locus_tag="IWM35_001974"
     CDS             complement(11265..11540)
                     /locus_tag="IWM35_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9919819.1"
                     /translation="MKLEDKKVILKKIKRLPDQIDGYTICSFKKDRSIKAYTQNTEKG
                     IIYFLHEEGYDTQTFMFTEKKEFHKQMKKLIEKEFPRSHKLYVNQQF"
     gene            11834..12649
                     /gene="thiD"
                     /locus_tag="IWM35_001975"
     CDS             11834..12649
                     /gene="thiD"
                     /locus_tag="IWM35_001975"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HAP9919820.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
     gene            complement(12978..14429)
                     /gene="cls"
                     /locus_tag="IWM35_001976"
     CDS             complement(12978..14429)
                     /gene="cls"
                     /locus_tag="IWM35_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HAP9919821.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQRALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
     gene            14770..17436
                     /locus_tag="IWM35_001977"
     CDS             14770..17436
                     /locus_tag="IWM35_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAP9919822.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            17800..18552
                     /locus_tag="IWM35_001978"
     CDS             17800..18552
                     /locus_tag="IWM35_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP9919823.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(18574..19728)
                     /locus_tag="IWM35_001979"
     CDS             complement(18574..19728)
                     /locus_tag="IWM35_001979"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HAP9919824.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYGLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
BASE COUNT         6330 a         3473 c         4060 g         5958 t
ORIGIN      
        1 aaaaaatgaa aaatcggtta attgtcgcat gtggaagtgg agtagcaacg agtcagacaa
       61 tcgctagtaa gatccaaagt atgttagaag atgacggcat cgcatttcct gtagaagcag
      121 ttgattacaa atcgattcaa aatgagcttc taactgctgg aatctatgtg tatgtcgcac
      181 aacctgatga ggaagtactt gaacaagcaa aagatttggg tattgaagta tttccaggaa
      241 ttccattttt aactggaatg ggtgtagaac ctatttatga cagtatcaaa gagttagtac
      301 agtaaaggag gattgttgga taaaaaagtt ggttcatagt tttatttact atatttagaa
      361 agaaagaagg tttatttatg gaagttttcc aaaccattat caattatgtt ttgaacttag
      421 gatcagccat ttttgttccc ctgattattt tgatacttgg attgttggca gggatgaaat
      481 taaaaaaagc attcatgtct gctttgactt tagggattgc ttttagcgga atgtctatgg
      541 tcattggcta tatgtctaac gcagtaagtc cagcatcaga agcattggct aaaaatacgg
      601 gaatcagttt gcctgcactt gatcttgggt ggacaggtgc agcaagcatc acatggtcgt
      661 ggagttatgc ttttatattt tttgcagtaa cgattggggt caattttgta ctgttacttt
      721 ttaattggac gaagacgtta aatgttgata tgtggaatgt ttgggggaaa gctttaactg
      781 cttatttagt ttattatgtt tccggtagtt tggctgcagg ctttcttact gcaatggtcc
      841 aagttatatt ggaattaaag cttggtgata tgtttcaaaa acatatacaa gatctcacag
      901 ggattccatt agtaacagtg actcatttta tgacatctgc cgctgtcttg ttgctgcctt
      961 tcaatatgat catggataaa attcctgctt taaataaacg agcggatacg aatgcattga
     1021 agaagaaaat tgggatcttc tctgaaaact ctgtaatggg attcattatt ggattggggc
     1081 ttggctttgc tgctgcgtat ggcgtttcag gttctcttaa tctagcgatc caaacagcta
     1141 cagcattaag tttgtttcct atgatctcta aaatgttcat gcaatctttg tctcctctag
     1201 cagacgctat gtcagaattt atgaaaaagc ggtttaagga tcgtgaagta tatatcggat
     1261 tggactggcc gatattagct ggtcgttcag aaatttgggt tactgctatt ttacttgttc
     1321 ctgtgtttat tggttatgcc attattttgc caggaaacca agtattgcca ttagcaggga
     1381 ttatcaacta ttcgattgcg gtcggcggat tacttttaac aggaggaaac ttggctcgta
     1441 tgctgatctt gggaatcatc actatgccta tttatttgta cggggccact taccttgcgc
     1501 caatactcag tgatttagca gaaaaaagcg gagcagtcga gggaatcaag aaaggacagt
     1561 tgatcacatg gtcatccata gaagggccgg agttcagggt tttatttgcg gaggcattta
     1621 ataggaatat tctcgctatt gtaggatgcg tgatttttct aggactgttt gttctacttt
     1681 ataaatatat gataaaagcg aaagtaccga gtgaacgtta tgctgaacta caaaaatagc
     1741 attgaaatgg tgggaaagta atgaaaagag aagagacaga taaaataaaa tggacagtcg
     1801 ctctttgtgg tacacttctg ctatttctat atggactctt tactcagaat atcatcatca
     1861 atctattggt catttttttc gcactagtga tctataaata tggaaatcac gttttattca
     1921 gagagtacga tgaaaaaaga aaacggaaaa tagaagaatc aatgaaaata aaagaagcta
     1981 ccaaagaaat attgagagaa aaaagtttta taaaaagata atggaggggt cattatgtca
     2041 gatggacgta tgttatttga gagagaacga caggacatgg cacgtatcgt aagagtgatt
     2101 ttcgaaagga aaaacacaaa tgtcgcagga gggaattttt cctttaaaac cactgatgat
     2161 caagggaaag actacatcat catgacgccg accatgatgt cagaagccta tctaggagag
     2221 atttctcctg cacaggttct agtagtagaa ccaagaacgc gtagaatcgt tgacggagtc
     2281 ggtcatttaa ctcgtgaaat caatatgcat gaagcgatgt atgagactaa tccagctatc
     2341 aaagctgtcc tacatgctca tgcgcctaat gcaatgttct gggcgactac tgggttgcct
     2401 atgcctaatt tgacagaagc cacagctaaa gtaaaagaga ttcctgtcat ggactttgag
     2461 cctaattgct ctgaagaact tgctgaattg acaagcgact atgtaaaaaa acagcatctc
     2521 gaattaccgc atatgttgct gctcaactct catggcgtat tgatcaacac aggtggagaa
     2581 tcaggaatcg aagctatcca taaagcactg caaatccttg atacagttga atggaatgca
     2641 gaaattgcct acaagcagac gatttttcaa gcattaggga ttactgacgg tttttattca
     2701 aaaggtgaga aaatcggggg attggaagag ttgaaacata aaacggctat ttataataaa
     2761 tctgttattg cagctggtgg agactaacct atctgacaca aaaaactacg actttcacaa
     2821 aatctgatcc agatcgatat ctgcttcagg ttgtattatg taagtcgtag ttcttttctt
     2881 ttatttagtt aagaggtggt ctgtccattc cctcgcttgc ttgatttcgt ccatcgtcaa
     2941 ctgatgacct gttacagtta gctgaatagt cacatttgct tggcgtgttt cgaattgatc
     3001 ggccaattct ttaaaggaag caggggaaac gattggatcg ttatcaccat aagaaatcca
     3061 gacggtttta tcctctaatg tggaacggct ttgatgaaca cctagagaca tcggatggaa
     3121 aaacaaccct ttatttagct cggtctcacg ctccaataat aaatgagcag cgatgtttgc
     3181 gccatttgaa tagccgacaa gcagccagtc acttaacggt atcagttctt tttcgctgat
     3241 ttctttgatt ttttcatgga gtttatctgt ttctttttcc aaactgtcca ggtcgaattg
     3301 attcaatcca ttacgcttga aaaaacgatt catcccatct tcttggaccg tgccacgaaa
     3361 agaaagcaat gtactgtttg gtgcaagaaa gtgagcgata tccagtaaag aatgttcatc
     3421 gccacctgtg ccatgaagta agatcaaatg cttgccactt gttccttttt caaaaatgta
     3481 ggtcattttg ttccctcttt tctgtttcta tagatgcaaa tgaagtagca tgaagagatt
     3541 attcacgttt tttatttgca tcacttgtat cgaaaggacg gacataagct tcaatggctt
     3601 cacgacgttc ttctaagaat ggaggaagag atagtttttc tcctgctgat tctgctggtt
     3661 catcttcaag gaatccagga ccatcagttg ctaattcaaa cagtacgtga tgagcggcta
     3721 ggaagtactc agattggaaa tagaaacgat cgacgaaacc agaatttggc aattcaatct
     3781 tattgattac ttgaatccag tattccaatg cttctttgtc tgctacacgc aatgctaagt
     3841 gatggatatt tccaaatcct tcgattgctt ttggcaagtc tgtccgatgt tcaataatga
     3901 tgcttgcacc atttccccct tcaccgattt caaataagta gaaatctcct gaatggtctg
     3961 tttctttgaa tcctaagatt ttttcaagaa tcaagcggat atgctctaat tgactgacac
     4021 ggacaaagac aggtccaagt cctgtgatcg caaactctgt cggtacattg ctgtttttcc
     4081 aaggaatgcc agctgctaca ccttgattgt tttcgtcaga gatcaattga taatactgat
     4141 tatcgaaatc ttcaaattcg atatattttt tgccgaaacg ttcattgatt gctgaatgag
     4201 taacgtcata tttttcaaaa cgttccaacc aaaagtctaa tgctgcgtca cttggcacac
     4261 ggaaagaagc acgagagatc gtatctgttc ctttttttcc ttttggctga tttggaaaat
     4321 caaagaaagt catatctgtt cctggcgcac cagtatcgtc tgtaaagtaa agatggtaag
     4381 tttcgatgtc atcttggtta actgtttttt tgattaagcg cattcctaaa atatctgtaa
     4441 aaaagcggta gatttttgta gcgctagatg tcattgctgt gacgtggtga attcctctga
     4501 tttgtgtatc catgataaat ttcctcctgt taacttacaa aaagtaagta gttgatttag
     4561 acatagtata gagggtgcat ctctattttg caattaattt gtcttctaaa gtatcaggaa
     4621 ggaatcaaga atggaaaaga ggagggacgg atcaataaaa taaaaagact tacgactagc
     4681 tgtatcgatt tcaaaaaaac tagctttaaa aagctaactt tagaatcatt acgggtggtc
     4741 ataagtcttt ttatgctgag ttattttttc atttttgagc taatgcttag gatagaatag
     4801 aatgttgatt tcacgatctc ttcttgttac ttgaaactgc ttaccactgt cacgatctct
     4861 tggtaaacgg ctttttcaca acctctttgg ttaagttgaa cgcacatcca gcacagaata
     4921 gttccgaaca tcaaatcggt ttcggctgga agaatattca atcggctgtc catctttgag
     4981 atagacgact tgctgaactt ccagtacagg atcgttttct gcacagttca aatattgctg
     5041 atcatattca tcacttttat ctgcagaaat agcacgataa gcccctgcaa aatggacatt
     5101 caattcttga tgaagatgat catagataga ggctaacaaa tgttcttttt ttaaacccgg
     5161 cacaagatgg actggcataa aggtatgctc taaaacatat ggctcgccat ctaagatcct
     5221 taaccggtga atattgtaga caggttgctc ggcatcgatc atcaatcgtt cttggatata
     5281 ttgattaggg aattcaacat caaaatgaat gatttgactc ttcaaagaac gatgtctgcg
     5341 tttcatttgg agactcaaac cttcatactc gttagcgggc gatgtgtctt tttgcataaa
     5401 tggatgattc aagattttcg ttcctgatcc tcgctgcgaa tagacaagtc cttccattgc
     5461 taagatattg atggcttttt tgattgtcat ccggctcgca ccaaattctt ttaccaagtc
     5521 gacttgatta ggcaaaaaac tatcgagagg atactctttt gaacggatac gttctcttaa
     5581 aatatttgcg atttcttcgt attttggcac gttattcacc tcttctagag tataacgaaa
     5641 gattatgctt ccgacaactc gaagtagcat ctggaacatt gtttattcga aaaaagaggt
     5701 catgacaagt ttttgtcaca accccttaat gatctattaa gcaatatcta aatctggttg
     5761 tttatctagt actttcagga atgggtacca gataaaggca acgatgacaa agtcgatgac
     5821 ttgcagcata ccaccaagcc atgaatttgt tgctaagaca ccgcttgcaa tgattggcac
     5881 agtccatgga acagagacgc cagttggtgc agggacaagt ccagcagcca ttaccaaata
     5941 attgaacgtc gttacgatta gaggtgccac aacccaagga ataaggattg ttgcattcaa
     6001 tacgatcggt agaccgaaga tcgctggttc atttacattg aaaataccag gtgctaaagc
     6061 taatcgtcca acatcacgat actgtttctt tttaagaaca aaggccatca ataaaacgac
     6121 agctaaagtc attccagagc caccaatacc aacagtgaag gtttccatga atggttttgt
     6181 gatgatgtgt ggtaattctt ttccatgttg ataagcttct aagttatcaa gcatcagtgt
     6241 attccaaatc gggtccatta cagagttgac gatgatttgt ccgtgaagtc cgaagaacca
     6301 taagaattgg acaaagaaca aagcaatcaa tgttgcaggt aaactacttc ccaaaccagt
     6361 taatggtttt tggatcactt cataaacgac gtcatgtaag ttagcattga aaataccagt
     6421 catcacagca ttgattacta agaaaatagt tagtgtcact acagcaggga tcaaagcggc
     6481 aaatgatttt gttactgcag gcggaactcc atcaggcatt ttgatctgcc agccgcgttt
     6541 cgtgattctg cagtagattt ctccagccaa gaaagcagcg atcattccaa tgaacattcc
     6601 ttttgcgcca agacgatcaa gtgaaagcac accagtcaca tcttcaccgc cagcggtttt
     6661 cattgcaaaa ggcgtaagaa tcaagaagct ggcaaaagac acggcaccac cgaaaatacc
     6721 ttcaacgtca taagattttg ataaataata gccgatacca aaagtaacaa aaaccgacat
     6781 gatgctcatt gttgcatttt gcccattacc gaataaattc gataaagttc cctttgttgc
     6841 gtcactgaaa aaaggcaagt tattgataac gacaacgatc gaaccgaaca tcgttaaagg
     6901 gaaggaaagc atgaaagcat cccgcagcac tgtaaggtaa cgattttgcc ctaataaatt
     6961 agctaagggc atgattttct cagaaagctt gtccataaaa ccgttcattt ttattcacct
     7021 caaatttatt tgcaatcgat tacatacaaa gtataacgcc aaaaatttta aatgtctagt
     7081 cattttcata aaaagtattg atttattttt tagggttcgt tatgattacg gtatcaagaa
     7141 aaaagagagg aagtaaaaaa atgaaaacct acacattccc agaaaatttt tggtggggtt
     7201 ctgctgctag cggaccgcaa acagaaggaa gagtaagtga tgatggtaaa ggagaaaata
     7261 tttgggatca ttggtatgaa ttagagcctg aaaaattctt cgatcaagta ggacctgaaa
     7321 aaacttcgca agtttacaca aaatacaaag aagatgttca gttaatgaaa gaaactggcc
     7381 ataacacatt tcgtacatct atccaatgga gtcatttgat tccagaaggg acaggtgaaa
     7441 tcaatcaaaa agctgtttct ttttatcgag attattttac tgaattgctg gataatggta
     7501 ttgaaccttt tgtgaacctt taccatttcg atatgccact accactgcaa gaaaaaggcg
     7561 gatggttatc aagagaaacg gtcgacgctt atgtttctta tgcagcagcg tgctttgaac
     7621 tgtttggaga tctggtcaaa aaatggttta ctcataatga accaatcgta cctgtagaag
     7681 gaggctactt gtatcaattc cactatccaa atcatatcaa tatgaaagaa gcggtccaag
     7741 taggctatca tgagaatcta gcaagcgcaa aggtaatcaa agcctaccat gaaggcggac
     7801 atgatggtga gatcggaatc atcttgaatc tgacaccaag ctatccgaga gatgaaacga
     7861 acccagaaga tgtaaaagcg gcgaagatcg ctgacgcatt cttcaaccga tcattccttg
     7921 accctgcagt gaaaggagag tttccacaag agttgatcga aattgtcaaa gaattggcta
     7981 taatgccaga gatccatgaa ggagacctag aactcatccg tgaaaacacg attgacctgc
     8041 taggaatcaa ttactatcag cctcgtcgaa tcaaagcaaa agaaactcca atcaatcacg
     8101 aacatagccc catgccagat gattattttg ataattacga catgcccaat aaaaaaatga
     8161 atccatatcg tggatgggaa atttacgaaa aaggaatcta tgatatttta acgaatacaa
     8221 gagaaaacta tggtaatatc aaatgctata tttctgaaaa tgggatgggg gtcgaaggag
     8281 aagaacgatt tgttaacagc gaaggaatta ttgaagatga ttaccggatc gagtttgtta
     8341 cggatcattt gaaatacgtc catcaagcaa tccaagaagg aaccaattgt gtgggttacc
     8401 acatgtggac atgtatggac aactggtctt ggacaaatgc ttataagaat cgctatggct
     8461 ttatcgctgt tgatttggca caagacggta aacggacgat caaaaaatca ggtcgctggt
     8521 ttaaaacggt ctctgaaaaa aacggatttg atgcctgata ttttttgata aaacgttcac
     8581 aaaatgtttg ttctaacact gtaatcgtgc tacactaaca gtgaaggagg cgtttacaat
     8641 ggtagatact tatcgtaata ttttagtagc aattgatggt tctgaaaagg cagaaaaagc
     8701 atttcttgag gcaatcacga ttgccaaacg aaatcaggca acgttgcaca tcctgtatgt
     8761 caatgaagta acaggcaatt actttggtga ttttgcattt gttacaacaa gccttcagga
     8821 agaattggat gaggttgcgg aaaaccagat gaaagaacat cgaaacttgg caattgaaaa
     8881 aggcctgaca gatatcgaga catatgttct ttacggttat ccaaagacac tgattgctaa
     8941 tttccatgaa tcgaaagaac caatcgatct gattgtcatg ggggcgacag gtctgaatgc
     9001 ggtagaacgt gcactagtag gttccactac ttcctatgta gtcaaccacg catcatgtaa
     9061 cgtcatggtt gtaaaataaa taaattaagg gaacagaaag caaaaaagca taacgcttct
     9121 tttgctttct gttcctttta tttttttgta aaaaattact ttacctgatg aagtaatcat
     9181 gctattctaa ttacaacgac agatggagta atatggagga aagaaaatga tttcaagtga
     9241 tggaatacgc ggatacaatg atgcgattat tttatcgatc cttaacaaaa acgattctta
     9301 tggatatgaa atttctaaaa cgattcgtga gaggacaaat gattcctatg tgatcaaaga
     9361 gacaacactt tactccgttt ttgcccgttt agaaaagaat caactgattg tttcgtatcc
     9421 tggagaagtg actcatggaa aaaagcggac atattataaa atcactgaaa tagggcgtaa
     9481 tcttttagaa gaaaaaaagc aagagtggct gacgacgaaa gacgtagtcg atcaattttt
     9541 ggagggaaat taaatggata caatccgtaa ttatttagac agtcttttta tcggagtacc
     9601 tcaaagtacc gagatagata agctgaaaac tgatttgttg gcaaatatgg aagaccatta
     9661 tcatgaattg atgggagaag gaaaaaatga acaggaggcc atcggtacag taatttcgac
     9721 atttggttca atcgatgaat tattagaaga actagatgtc gaaaaaaaac atcaagctga
     9781 cgagacggaa acaaacacag cttctattta tttaagcgaa gcagaaaact actggaaaga
     9841 atacagagcg gcaagtctcc aagtggcatc aggtgttcta ttcattagct tatcttttgc
     9901 aagttttctt ttcttttgtt cagcaggata tgtgttcatg ggtatttctt gtctgatttt
     9961 cggtatagca ttagctgtcg gtttttttat tgcttctggt atgaaaatca cacgtttaaa
    10021 tcactttttg caccatcgga aaatacctga aaaagtatta gcagaagcaa aagaaaaaga
    10081 agaagagtat cagcgaagtt ttggcttctc actgattgca ggaattggat tatgtatttt
    10141 ttcacttttc ccacttttgg cttctttaat gtggtatatg gatggtagta tcggtgcctc
    10201 tatatttttt gttacggtag gaacgggtgt cttcttgatc atttatggca gtctagtccg
    10261 tcattcttat cgtcaattca ctcaaagcgc ttattactgg tgaatgtatt cagatttgta
    10321 aaaatattct atttatccgg cgtagaaaaa aagactgtga cacgcttttg tcacagtctt
    10381 ttttatatat aataatttaa aaattcatca tctaaatccg tagaattttc tagattgctt
    10441 acccatgtaa aatcgttata gacagatttc agttgacttc tgcgccattg tacagggata
    10501 aaaagacgca tgctatcttc attgaatccg actaataatt tctttccgtc agtaatgatg
    10561 ggacgttgta acaaagtaaa atgcgagacg atcagatcga caagctctga ggtcgtacat
    10621 tggtcgattt ggtctttcaa atttttataa tcaatcgagc gtttagctaa tagatcgtca
    10681 aatccgtttt ctgttaatga caataaataa aatatctctt tccttgttaa aggctgttta
    10741 ggtgtgaaaa ttcgttctct gtaaggcatt tgatggtctt tcagccagtt tgccatcaga
    10801 tggtaactat gtgtgcgaga agaatagatc gtaagcataa aaaaactcct taaatagtcc
    10861 attaatttgt tagatctgtt gagataaata ataggtaagg agctgtgcat aagcattgat
    10921 caaaacaacc caaacatcat tagaaataac tttctaaagt cttaaacttg cgacgagaaa
    10981 caactagact aatagaaaaa atgtcttagc catttggctt cctgtttgtt tttttgctta
    11041 tgctgcagct cctcgggaaa gggttctaac aatttgaaga aggttgatga ttgatctagt
    11101 ttaaattgtg tgaaatttga aatctggtgt ttttactaga tgaatcacct aacaactatg
    11161 attataactt atagaacttc tgttttgcat tcggttttgt tatagttgtt ataaagtttt
    11221 attttattac agaacatttt taattaataa agaacagtta gaagttaaaa ttgctgattc
    11281 acatagagtt tatgacttct tggaaattct ttttcaatca gttttttcat ctgtttatga
    11341 aattcttttt tttcagtaaa cataaaggtt tgggtatcgt aaccttcttc atgtaggaaa
    11401 tagattattc ctttttctgt gttttgcgta taagctttta tactcctgtc ttttttgaaa
    11461 gaacagatcg tatagccatc tatttggtct ggtagtcgtt tgattttctt caaaatgact
    11521 tttttatcct caagtttcat ttttttcacc tgactttatt tttttcatat ggaaatcata
    11581 gcgcctttgg ttatatgaaa acaagaagga tactgaaata tatataaatc aaatagtggt
    11641 tatttattat tagaatagcg aatacaaata aacgaccact attttctgat ttattgcaaa
    11701 tgttttttca aataaatttg attaccaaga aaaggtagtg gtatatttac tgagggatac
    11761 tttgaaaaac atatgataga aacagtaaca gtattatgaa aaatcttttt ataaaaatgg
    11821 aggaatcgat ttgatggaag atcagtatgt acaagcaatg acgatcgcag gatcagatag
    11881 cgatggaagc gcagggatgc aagcagatat gcatacattc tttacaagaa atgtttacgg
    11941 tgtttctgtc atgaccgcat gtgttgcagg taattcttac ggaatcggag caagtgtgac
    12001 attgccaaca gattttatcg acaaagaatt tgaactgatt gcgaaggact ttcaaatacg
    12061 tgcagctaaa acaggtatgc tagcagattc aaagttgatt gagacagttg taaaaaatta
    12121 ccaaaaatat gattttggac cattagtcgt ggatcccgta atcgtaacga aacatggaaa
    12181 tcttttacta gaagaatctg ctttgcaaag cttgaaagaa aagcttgtcc ctttggccga
    12241 agtactgaca cctaattttt atgaagcaga aaagctgact ggacggacat tagcagctga
    12301 agaagatttt gtgcaagcag caaaagaact gcaagatatg ggtgcaaaaa acgttatgat
    12361 caaaggacat catgctaaaa atgcgcagca agaaacagtc aatgactacg tattgttcga
    12421 atcaggaaaa tcattttggt taaatgcacc attttatcct accgaccgga tcaacggaac
    12481 tggcgattcg ctatctgcat gtattgcagc tgagttagcg aaaggaaaaa cagttgaaca
    12541 agcgatccga ttggcaaaag actatgtaaa ccgtgcaatt cgtgaagaaa taaatgtagg
    12601 gcacaaatat ggccccatca atcattgggc agctggtgaa ttagagtaaa agaaagaagt
    12661 tgtgaaaaag ttgccctgca tcaagtaata agaacagcca agtaaaaaca gcttcttatg
    12721 tttttaactt ggttggactt attaccgcag atgcaagctt ttgaacaccg tttaagaaag
    12781 aggtcgtgag cctgccgtcc agctccgagt cacaaggaac aagttcaaaa aacagcttct
    12841 catgtttgtt gaatattgtg acttgtgacc gaggagttgg caggagaaca ccgtttgttc
    12901 ggaagaaggg actgtgacaa gatttttgtc acagtccctt taatcttagt atatttttct
    12961 ctacttgaat aaaaaaatta taggattgga gaaaataatc gagaaatttg ttgcttgaat
    13021 aatagccagt tagacatttc cttgactgtt tcagttgtca tggatgaaca atgtgtctca
    13081 tcttctttga actgagtagc aagttcttct aaaaactcag gatcgtaaat gacagcactt
    13141 gtttcaaaat tcaatcggta gctacgaata tcttgatttg ctgaacctac catgcagatt
    13201 tcatcatcca ttatcaatgt ttttgcatgg aggaagccgt tctcatagac aaggatttgt
    13261 acattttcct tgatcagctg ccgagcataa tattgtgttg ctcgataaat aaaaggatga
    13321 tccggtttgt tgggaatcat gattttcaca tctacaccag aggcagttgc gatttttaaa
    13381 gcagcaatga cactttcatc aggaacaagg taaggtgtct gtatccaaac acgtttttta
    13441 gcagaagtaa tcaatttgat aaatgaaagc ttgatttgtt ccctttcgtt attcggtccg
    13501 cttgaaacaa gctggatgga tgtatcagca agcttagatt catctcgatc tgctttttta
    13561 aagaaataat tcaaatgata gccgaccttt ttttcttcag gtaccgagac gttccagtct
    13621 gttaagaagc gaagctggag caaagaagaa gcggcaccga aaatccgtat atgcgtatcg
    13681 cgccaatagc cgaatttttt tgttgtattt acatattgat tggcaatatt gaaaccaccg
    13741 gtataactaa tctttccatc gatcacaacg atttttcggt gatcatggta attcaatcgg
    13801 aaacgaagta atgccctttg tgaggtaaca aatgtatgga catgtccacc atttttgatt
    13861 agacgattga aatcttttgc ttttgtgcca tgagagccaa atgcatcata taatattcga
    13921 acttccacgc cttctgcggc tttttcttct aaaacatgta agacttgctg gccgatatta
    13981 tctgtcacaa atgcgtaata ttcgatatgg atcgaatgtt tggctttttt tagatcttga
    14041 agcagtgaat ccaatttctc ttgtccgtct gtgagaagag tgacagaatt cattcttgtc
    14101 agcggcatac gatttaatga agagaagaag tcaacaaatt gctgtttgtc tttatcgccc
    14161 atgtcaggat cgtaatgttc aatggtatct cctctaaaag actgaaagtt ttctaattct
    14221 ttcaagtcac tttgtcggag atagaatttt ttcttatccg ttaatccacg tccgaaaaat
    14281 aaatataata caaagcccac cccaggaagg gcaaacaata ctaagagcca tgcccaaatg
    14341 gctgctacat ctcggggttt gatcaaaata gccaccaaag caataagtgc atttaataga
    14401 taaagggcgg ttataatact agataccaca aaaatgttcc tccttacata caataacatt
    14461 agtatagcat gatagaagaa agaagcctac caaggtgaat atacttttca ctcctttcat
    14521 gaaaatgtag gtaaatcggg ctaaagtagt tgctttttgt aggttaataa ggtaggatat
    14581 tagtacagtt gaatatctcc ttaggggagt aaccggcaac gtttgttgcg ataatatcaa
    14641 cagtaagtta accaaatggt tttctggtat tgtatgaaat ggtgagacct aagacaaatg
    14701 cgtatttgtc ttaggtctct tttttttcag acaggactag ggccaagatc aaaaaatggg
    14761 ggatgaaatt tggaaaacaa ggaacaagca caacaagcgg tctataaaga aacgatcgac
    14821 tcgttgatgc agcaaacgca gagctcaacc gatggattga gcaccacgca agcgaaggaa
    14881 aaattagaaa gagatggctt gaaccagcta aaagaaaagc cgaagaagcc aacttggaaa
    14941 ttgttcttag aaactttcaa agatgcaatg gttatcgtat tgctgattgt cgcactgatt
    15001 cagatggtca tgggtgacta catcgaattc ttagttattt ttgcagtatt gatgttgaat
    15061 tctataatca gtgttgtcca aacaaaaaaa gcagaaggtt ctttagaagc gttgaaaaac
    15121 ctttcagcgc ccgatgctcg agtgattcgt gatggtaagg aaaaaacgat tccggcaaac
    15181 gaacttgtgg taggagatat cgtgttgtta gacgcaggcg attatgtacc tgctgatgga
    15241 cgtttactag atgcgggatc attaaaagta gatgaaggta tgctgacagg agagtcgact
    15301 tctgcagaaa aagaagtaac ggatatcaca agtacggtac caatcggaga tcgtacaaat
    15361 atggtattca gtggaacgat cgtaacttac ggacgcggta aattcttagt gacttcaact
    15421 gggaataata cggagatcgg acaagttgct ggccttttgg aatcaacaac tgagcaagtc
    15481 actccgttac aacgaggtct ggataaattc agtaaaaaac taagtttggc tattttggca
    15541 ttgtctttat tgattttagg tatccaactg tttcgtattt atcttggaga aggtacagga
    15601 gatatgactg cagatatcgt cagcgcagtg atgtttgccg tagccgtagc cgtagctgct
    15661 attccagaag cattacagtc tatcgtaaca atcgtgttat ctttgggaac gaataaaatg
    15721 gcaaaacgtc atgccattat tcggaagctt ccagcagttg aaacacttgg ttcgacaagt
    15781 atcatctgta cagataagac tggtacattg acacaaaaca aaatgacagt tgttgactac
    15841 tttttaggta atggcgatac tggggatttc acaaatgacc cttcaaaatg gacagctgac
    15901 gaacgtcgct tgatcgaaat cagtgtttta gcgaatgatg catcgatcag tgaagacggt
    15961 actaaattgg gcgaccctac ggaagtagcg tttatcgact ttagtgaaaa attaaaccag
    16021 ccttatcagg aaatccgaaa caactaccca agacaagcag aacttccttt tgactcagat
    16081 cgaaaattaa tgtctactgg acacacaatt gataacaaaa attacttgtt tgttaaaggc
    16141 ggaccagata tcgtcttcca acgcagtaaa aaagtattgc taaatggtga agtcgttccg
    16201 atgacggatg aattgatgaa aaaattccaa gagcaaaatg aagctttttc aacacgcgca
    16261 ttacgtgtac tagcttttgc ttaccgtcca cttgataatt ttgatttatc atttgaagat
    16321 gaagacgatt tgatccttgt tggtttgctt gctatgatcg atcctccacg tgctgaagtg
    16381 actcaggcgg taacagaagc aagaagtgca ggaatcaaaa caatcatgat tactggtgac
    16441 cacaaaacaa cagctgttgc tatcgcaaaa caaatcggga tttttgaaga aggaaacctg
    16501 gctttgaccg ggacggaact agatgaattg acagaagatc aactaaacga agttttagaa
    16561 aaagtcactg tttatgcacg tgtttcacct gagaataaaa ttcgtatcgt tcgtgcttgg
    16621 caaaataaag gtcatatctc agctatgaca ggtgatggtg taaatgacgc accagcattg
    16681 aaacaagcag acatcggaat tgctatggga acgggtacag atgtggcaaa agatgcagca
    16741 gcgatggtat tgacggatga taactttgct tctattgtca gcgcagtaga agtaggacgt
    16801 aatgtgtttg ataatatcaa gaaagctatt tcttatctat ttgcaggaaa tttaggtgcg
    16861 atcattgcga tcatctttgc cttagttgct gactggtcca atccatttac agcattacaa
    16921 ctgttgttca tcaatttagt caatgattca ctgccagcaa ttgcattagg tacagaaaaa
    16981 gctgaacctg ctacgatgaa ccgcccgcca agagatccaa attcaggaat ctttacggac
    17041 aaaacattga tttctgtaac gtatcgtggt gtgctgattg ggattgctgt tatctttgct
    17101 caatggcttg gcatgcagca ttcacctgaa ttcggtgtag ccatgtcatt ttcaacattg
    17161 atttggtgtc ggacattgca aacattccca gcacgttcaa acacacaaac agctatccaa
    17221 gcagggttct tctctaacag aagtgtctta ctagctgtag ttgtatgtag cgcgctttac
    17281 tgcttaacaa tgatcccagg actgaaagaa gtcttctcta tcccatcagc atttggattg
    17341 acagaagtct ttacatgtat gggaatcgca cttgtgacaa ttgttttgat ggaaatcact
    17401 aaattgatca ttgtacgagt acaaggaaat aaataaaacg aatcaacgag gttgtgagcc
    17461 tgccgtttaa ttctgagtca caaggtgcaa tttcaacaaa cagcttctca tgtttgttga
    17521 atattgtgac ttgtgaccga agagttggca ggagaacacc gtttaaggaa gaagttgtaa
    17581 aatcagcatt ttgagctaat actgagggtc atctgattgg acactgttta ttcggaaaga
    17641 gggactttga cacgactttg gtcacagtcc ctttttttat ttatgataga cggaaagcca
    17701 atcattttat tattcatcaa aagagataga atagataaaa gaggagaatg atttattccg
    17761 aaaagtgaaa acatataaca aaaaattaga ggaaatcaaa tgaatgaaaa agaatgggat
    17821 gaatttgctg aagaatatta tgaaatccag caagaatcgc aagtaactat tgctgaagat
    17881 gtaaaaaatt atctgaaaac ggagaaagtc ctgcctgctg aaacagcagc agacgttgct
    17941 ggaggatctg gcagatttct ttccttagca aaagaaatta atcgctatga tttgatcgat
    18001 ttttcttcag gaatgctaga aaatgcaaaa aaagaagcag aaaaaaatga gttgccaaat
    18061 gtttccttta tcaaacagtc gttagaagaa tttatccaat caaatgagac atatgaaatg
    18121 gtttttacag cagcaaatcc agcattggta catcttattc aattagaaaa attgatgaaa
    18181 aagtcaagca aatggtgcgt gataatcaga gtagtggaat caatcgatga tgttttttat
    18241 cctatcgaaa agcaactagg aatgatagaa gaagatccta atacatcgcc agttgtaatg
    18301 gatcaatttg aaacatactt aattgatgaa ggattcaaag tgaagaaaaa agattttact
    18361 tattttacac aggaatcaat tactgaagaa ttgatgaagc tatactatga cgcgtacgca
    18421 aaagatcctg ttgtgaccag gtacattcgc aaactattca gtgaaaaacg agaaatcctt
    18481 agtacgacta agctgaccta tagactgctc ctttggtccg cagataaaaa aagaaccttt
    18541 cacgactcgt gaagggttct ttttatgatt ttattatatt ttgtagtaac gaatatcatt
    18601 ttcgatcttt gtaatcgaaa aaggtacatc tgaagtatat tctgcacatt ctccgtgttc
    18661 aggaagcgtc tcactgaaaa tcgtttcata ttcgtcaaca gtgatccgtg ttcgagaatc
    18721 tagcatctct tgatgagatt gtgcgaagag atattcttga tagttttctt ccagataacc
    18781 tgtaaaaaat tcacttactg caccagaacc gtagctgaat agaccgatgc gatctcctgg
    18841 ctgtagtgat ttcgaatttt ccagtagaga agttagcccc aggtataatg aaccagtgta
    18901 aagattaccg attcgtcggc tgtaacggat gctttcttca tagcgagcca taagacgttc
    18961 ctgattttct tcgtctgttt ggtctaatac gctttgcaat gccttttttc ccatcttagt
    19021 atacggaata tggaaagcaa tcgcctgata atcttcgagt cctcgacccg acaattcttt
    19081 atgtcgattc caaacttttt ggaatgattc gatatacgta gaattagata aaggaccatc
    19141 aacaacagga aattcgctat aatctggacg ccagaaatca tagatatctt ctgtcagaaa
    19201 tacgctgtcg tcttcaatcg ataaaatacg cgggttttga gtgatcatca tcgcaacagc
    19261 accgacacct tgcgtcactt caccaccgct tgccaagccg taacgagcaa tatcacttgc
    19321 tatgactagt acttttcgtt ctggatgatt tttgacatat tctttcgcca tgtgcaaagc
    19381 tgcggtgcca ccgtagcaag cctcttttac ttcaaaagaa cgagcaaaag attggatttt
    19441 cagtaaatgg tggataatta ccgcactcgc ttttgaatga tcaatcccgg attctgttcc
    19501 gacgatgacc atgtcgatta gctcacggtc tttttctgtt acgattttgc tggcagcatt
    19561 tgctcctagt gtaatgatgt cttcatttgc acgattgact gccatttggt cttgaccaat
    19621 ccctatatgg tattttgcag gatcatcccc acggctttct gccaactctg tcatatctaa
    19681 atataaatta ggaataaaaa aggaaagacg atcaatccct attttcattt caacacctct
    19741 ttacttatga taaaaaactt actttactaa ggtatcataa aaagattaaa agaaatataa
    19801 aaaagagaat atttaaggat t
//
LOCUS       DACDXO010000050        19472 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E23605_2017
            SAMN12639989-rid9881463.denovo.051, whole genome shotgun sequence.
ACCESSION   DACDXO010000050 DACDXO010000000
VERSION     DACDXO010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639989
            Sequence Read Archive: SRR10023389
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741914.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:42:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,972
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 33 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 17 of 73
            Pseudo Genes (multiple problems)  :: 10 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19472
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639989-rid9881463.denovo.051"
                     /isolate="E23605_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(20..994)
                     /locus_tag="IWM35_001980"
     CDS             complement(20..994)
                     /locus_tag="IWM35_001980"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HAP9919825.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            complement(1074..1949)
                     /locus_tag="IWM35_001981"
     CDS             complement(1074..1949)
                     /locus_tag="IWM35_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HAP9919826.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSGKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            2079..3986
                     /locus_tag="IWM35_001982"
     CDS             2079..3986
                     /locus_tag="IWM35_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family peptidase"
                     /protein_id="HAP9919827.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            complement(4527..7805)
                     /locus_tag="IWM35_001983"
     CDS             complement(4527..7805)
                     /locus_tag="IWM35_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1565 domain-containing protein"
                     /protein_id="HAP9919828.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEVPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLESYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
     gene            complement(8076..8621)
                     /locus_tag="IWM35_001984"
     CDS             complement(8076..8621)
                     /locus_tag="IWM35_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP9919829.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDAVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            complement(8633..9325)
                     /locus_tag="IWM35_001985"
     CDS             complement(8633..9325)
                     /locus_tag="IWM35_001985"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HAP9919830.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYKPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(9344..9628)
                     /locus_tag="IWM35_001986"
     CDS             complement(9344..9628)
                     /locus_tag="IWM35_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9919831.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(9641..10204)
                     /locus_tag="IWM35_001987"
     CDS             complement(9641..10204)
                     /locus_tag="IWM35_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HAP9919832.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            10432..11094
                     /locus_tag="IWM35_001988"
     CDS             10432..11094
                     /locus_tag="IWM35_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     protein"
                     /protein_id="HAP9919833.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            11218..12414
                     /locus_tag="IWM35_001989"
     CDS             11218..12414
                     /locus_tag="IWM35_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HAP9919834.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(12515..13891)
                     /locus_tag="IWM35_001990"
     CDS             complement(12515..13891)
                     /locus_tag="IWM35_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP9919835.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(13928..14794)
                     /locus_tag="IWM35_001991"
     CDS             complement(13928..14794)
                     /locus_tag="IWM35_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose transporter GlcU"
                     /protein_id="HAP9919836.1"
                     /translation="MEILIALVPMLAWGSIGLVSGKIGGDANQQTLGMTIGAFLFSLV
                     VFFVVSPVITPWIFLIGFLSGLAWSVGQNGQFHGMKYMGVSVGLPLSTGFQLILNTIA
                     GAVFFHEWTQTKDYVYGIIALALLVSGAYLTARQDDEGKIDTDNKMLDFGKGFRALIF
                     STIGYGVYTIIVNWANLDAMSIILPQSIGMILGASFFAFRKVKVDQFVWKNMICGLLW
                     GLGNICMLLTVKSLGLAVGFSLSQMGIIISTLGGIFILGERKTKKELIYVIVGCLLVI
                     LGGILLGYMKTV"
     gene            complement(14883..15908)
                     /locus_tag="IWM35_001992"
     CDS             complement(14883..15908)
                     /locus_tag="IWM35_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP9919837.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(15926..16600)
                     /locus_tag="IWM35_001993"
     CDS             complement(15926..16600)
                     /locus_tag="IWM35_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9919838.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(16784..17677)
                     /locus_tag="IWM35_001994"
     CDS             complement(16784..17677)
                     /locus_tag="IWM35_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP9919839.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(17751..19448)
                     /locus_tag="IWM35_001995"
     CDS             complement(17751..19448)
                     /locus_tag="IWM35_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAP9919840.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLVKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
BASE COUNT         5684 a         4189 c         3445 g         6154 t
ORIGIN      
        1 ctttccctct tttttttgtt tattcgaatt ctttttcttc cagacggaat aacggactca
       61 tcggtgtatt ttcatggatg cgtttgaccg cttcacccat taattctgaa catgtaacga
      121 ttgttaagtt ttctggatga cgattttctt ctgtgaaaac agaatcagtg atacaaatat
      181 ctttgattgg tgcattgtct aaaatacttt tcgcatgatc ggacaataat ccatgtgacg
      241 cacaggcata tacttctttt gcgccgtttt cttttaatac atttgctgct cgtgcaagcg
      301 ttgctcctgt gttcaagata tcatctacca tgatacatgt ctttccttga acatcaccga
      361 tcacataacc accctcagtt gaagcatcat ctgcatgatc tacgatggct aatgtgctgt
      421 tcaaatattc tgaaagacta cgtgcacgct gaacaccgct gttttttggc gaaacaacta
      481 cgatgtcatc tcccgtcaga cctaattgac ggtaataatg agcaaacaac ggcatcgtaa
      541 ataaattatc tacaggaata tcaaagaatc cttgtacttg aaccgtgtgc agatctaaag
      601 tcagtactcg agttgcccct gcttcttcga tcaagttagc gatcaatttc gcagtgatcg
      661 gttcatgagg tttcgccgtg cggtcttgcc tagcataccc atagtaaggc aatacgacat
      721 tgatcgtttt cgcacttgct cgcttcattg catcgatcat gatcagtagt tccatatagt
      781 agtcattgac aggtgcattc gttgattgaa cgatatacac atgatcccct cgaatacttt
      841 cttcgatatt gatggaaatc tcgccatcac taaattgctt cacgacgctt ttacctaatt
      901 ctgttccgaa cactttcgcg attttttcag ctaatggacg gttgccattc aaactaaaaa
      961 tcttcaacgt gtcatcctga tacttattac tcatgtagtc ctccccaaaa cttctctatt
     1021 cttcgatagt ttaacatatt tttccaagat tttctgttaa tttcagcaaa ttcttaagga
     1081 agtgaaactg ttccgttact ccaactggca ggaatgactt tatgataatc gccccagttg
     1141 tcgtgtactt tgtaataacg ttctcctgtt tcagtttcaa aataagcgta cgctgtcacc
     1201 caatgattgc catatgtact gccaagtact ttcaaaatac caatcatcac aggtttgcct
     1261 tcacgtatac gttttgtagc tcgctgccat gaacctagaa gagttgctcg tgcacgctca
     1321 tgattgccat tcttctttaa aaaactagaa atccctgtac taacctgaaa aggaatcgta
     1381 ggaagtccta gtggttggat ctggctacgc aatgccttcg tcaaaaccaa cgaatcagcc
     1441 gtatttttct tgcgaatctc atttggaagc atccacccct tacgatagtc ttgataatag
     1501 gcaaggagaa ctgctgccgc gtaagtacca caaagatagc ctgagggggt agcccatttc
     1561 ttatacctca aaaaacgttc ttctggcaat cccacccagc aatttgcgag ttcctttcct
     1621 gacctttttt cttcgatcaa aacgggaata tcttcttttc tgttgatcca tctagataac
     1681 tcgattggct cttcattgat cagcttccat tttttacaag aaagaccctc ttgataagta
     1741 tattttgctg gagaatattc taccggatgt cctgattgat cgataatcac ataactttga
     1801 tcttcaaaaa ccagagctaa aaaaagcaat ttactttttc ttgactgcag ctgctgagtg
     1861 ctggctaatt ttttaatttt tttatctact ttgaaatata aaggtttatc cactacttga
     1921 ttcagttggg atcgcctcct ttttttcatt ataagtgaag gattgaaaaa aacaaatcag
     1981 tttgattgtt cgaattttca attttttatg ttctatttgt taattccttc attttgtaag
     2041 ataatgggca cacgaaacaa aaaaggagcg atcgatgaat gaacaaagaa cgattgaaac
     2101 aagatttcta tgaagcagtc aatgaagaat ggcaaaaaac agcaaaaatc cctgcagata
     2161 aaccagctac aggcggtttc caagatttag ttgacgggat cgaccaactt ttgatgggag
     2221 aaacagacga tctgcttgcc catccagaaa aagtgtcttc agaaaaaatg aaacatttcc
     2281 ttgcatatta tcaactggca aacgattatg accgaagaaa tgagttgcat gctcaaccgc
     2341 ttcaacctat aatcgaaaaa gttgaatcat tgcagtcttt cgacgaattg aataagcaat
     2401 ttccagaatg gctgttggat acattaccat tgccatttaa tctggatgta gatgcagata
     2461 tgaagaatgc gcaaacgaac gccttctttg cttatccgcc ttctttattc cttccagata
     2521 agacgtatta tgcatcagac catcccaacg gtgcacaact aataggcgtt ttctttgata
     2581 tggctgttca gttattagag cttgccggca aagaaaaaag tcaggcagaa gcaatcacgg
     2641 aacaagcagt gcaatttgat aaattgattg ctcctcatgt aaaaagtgca gaagaaaacg
     2701 cagattacag caaaatgtat aatccgcaat cttttgatac atttattaaa tacacagata
     2761 aactggactt gaaaaaactt gtcacgggtt tgattgggac aactccagat aaagtgatcg
     2821 taactgatcc agtgtacttc gatcatttga atgacatcct gacagaagct cattttccgt
     2881 tattcaaaag ctggatgatc attaaaacag tacgttctct tagtggttat ctatcagaag
     2941 aattccgcca agtcagcgga atctattctc gcgtattgtc tggaacagac gaagcaatgc
     3001 ctcagaaaaa agcagcgtat taccttgcct ctggacaatt cgatcaagta gttggcgatt
     3061 actacggaaa aaaatatttt ggcgaagctg ctaaaaaaga cgtagaacaa atggtgaaaa
     3121 aaatgatcgc cgtttataaa aaacgtctag aagaaaatga ctggcttggg gatgcaacaa
     3181 aagaaaaagc cgtcataaaa ttaaataact taggtatcca agtaggctac cccgataaga
     3241 tcccagcatt attcgaccag ttcaaaacag tacctgcaga tcagggtggt actttgcttt
     3301 caaatactct tgttttcagc aaactcacat taaaagatcg tttcagcaaa tggaacaaac
     3361 cagtggatcg ttcagaatgg gaaatgagtg ctgacacggt caatgcttat tatcatccat
     3421 ttcggaatgt gatcgtcttt ccggcagcga tccttcaagc accattttac agtctagaac
     3481 aatcaagctc tgctaattat ggtggaatcg gagctgttat cgctcatgag atttcccatg
     3541 catttgataa taacggcgct ttatttgacg aatatggcaa cttgaacaac tggtggacag
     3601 aagaagatct tacacaattt caaaacaaag cacaagcaat gatcgaagaa ttcgatggtc
     3661 tgccttttgc tgacggaaaa gtaaacggaa aattaaccgt ttcggaaaat attgctgatg
     3721 caggcggtct aagctgtgca ttggaagcag caaaaacgga atcagatctt tctatcgagg
     3781 aattctttgt caattgggca acgatttggc gtacaaaagc aaaaaaagaa taccaacaac
     3841 ttttactgca aatcgatgtc catgcccctg ctaaactccg agcaaacgtg caaccaaaaa
     3901 accttgacga attctatgaa acatttggta tcacggaaca agacgctatg tatctagcgc
     3961 cagaaaaaag agtgcgaatc tggtaagagg ttgtgccaga agtggtcagc ttcaagaaat
     4021 aagaaggaat ttgggaaaat cgcttctcaa atttttgtga catttcggct tatttttcga
     4081 agaagttact tctggaatac catgtattag aggttgtgaa aaagctgctt agtttcaagt
     4141 agtaagaaaa attttgtgaa aatggctttc cacatttttg cataaatttg acttactacc
     4201 gagaaactgg cttttgaaca ccgtttatcg agaggttgtg aaatcagcgt cctgacctga
     4261 gtattagaga aaaaaacgga aaaatagttc ctcatatttt gccgtttgtt gagctaatac
     4321 cgagggtcag ctgattgaac accgtttagt ggagagattg tgaaaaagct gtcctgcatc
     4381 aagtaataag aacagccaat caaaagcagc aaggagaacc tcatctataa aaagatagaa
     4441 ggaagtgaga catctattgg tctcatttcc ttctattttt actttcctca gtcaattgtt
     4501 ttacatcata aaaggagaaa ttccacttat ttttttcgct gtttcatgac ataaacaact
     4561 acaccgatta acaaaattcc tccagctaat gctagcaacg ggttattaaa ttctcccgtt
     4621 tttgggagtc cttgagtgcc atttttttca ctttcttttg ctgtcctatc cggatgagaa
     4681 ggttttccat ttggttttcc atttggtgat tcatttcctg gattttctct ttctggtttg
     4741 tcatttcccg ggggcacatc ggcagtttct cgcacagtga tcgctttttg ctgggtaaca
     4801 gattctgtat gagcagacca tttttcccca tcaaatattt ccgaacgata agtgacttct
     4861 agcgtatatt ctccttttga aggtgctgaa aaaatagcgg tatgattaga aaattgagca
     4921 cttgactgtg tttcaacgat ttggatcttt tctgggaaat aacgcatatc ttcaaagaca
     4981 ggcgttcctt gatttggtgt gaaagttgac acaaaaactg taaaaggctt cagttcaatg
     5041 atatttcctt ctgcattctc cgccacacca tttgcttctt gtataataat cggattattt
     5101 tgttcatttg gttttgtctg atttggttct tgtacgattg catactctgg atattccgaa
     5161 gacggtgagg tttctccgtc aaaaatagca cctacagata atggaagacc atccactctt
     5221 tccttttctg tttgacgatt ccccggttta atctgcatca gtccatgaac atggatactt
     5281 ccattttcat agcgcgtaac aggctgctga ccttctttcg aacatgtatt tagagactcg
     5341 atccaatctt ttgatatggt atgtccaaga gtattttctg attgtttctc tcgttcattc
     5401 caatagtagt tcaacgagtg gtagatttga tcttcgtttt gattcaatgg gcggatctgc
     5461 tctctcatct caagattctc tgttcggaaa gataagatac cagaagcatg gtagtctgtg
     5521 tttcctgctg aagaggtata aagagccaca ttatatgaac cgttattaaa acttgtactg
     5581 ctattgacga taatatctgt tccgctattg gaatcgattc ctttcgcaag attttcgaaa
     5641 gacaaactat tgatcaattc atgagggccc gttaaagaag aaccgcccat tttgaatcca
     5701 ttaccgttac cttctccctc aattcctggt acccagccgt ttcgataagc aactgagttt
     5761 ttgatgatta cttttccgat cgaacctgtt tcatttttag caaaaaggtc ccaaccgtca
     5821 tctgcattat gatacgcgat acaaccgtca aatacatttc cttctcctat tgtcagtttg
     5881 gccgcaaatc catcggcatc ttcaaatcct ggatcggcat tttcaaaaga tgtacagttc
     5941 ttgactaagt tatgcgcggg ccatgcttcg aaaggatcat ttgagctacc gctgatttgg
     6001 attcctgtat ttccattccg ataagcatga atctcttcga tcaaattatg atttccagaa
     6061 atctgtaatc ctttttgcat agctgcacta tttgtaatgt caaaccctcg taaaccccag
     6121 tagtgactga acagtgtcat cccatttcca gttcctttaa aatcaagtac aggacgcttg
     6181 ttttctggat ttttctctgc aatcaactga atcgggtttt ctcttgtccc gttaacgcct
     6241 tttggaatcg tcaatcctct ttcgaaggaa taagttccag gcgccatgac gattgtctgc
     6301 ccagcatatg cgtatctcaa tgcttgtgtc agagataatg gattttgtct gcttccgttt
     6361 cctgcatctg acccttctgg tgttacataa actgtcgaat atttaggttt acggtagtcc
     6421 actgtatgaa gaatctcttt tgtttcataa ctagtcatct ctacgtattc ttctggcgga
     6481 tagtttttat ctggcgtaaa actgaaagta aattcgttcg ttccttccat cagttttgtc
     6541 tgataatcaa attcccgatt cgcagtgata gccaagtttt ttgcgttctt aagcacctct
     6601 cctgttgctt tatttttaat tgtcaataat ccgtcagcat ttgctctaaa ggacatagta
     6661 tgatcttggt ttcctgaatg attactagag gtcacagtgt aaatcggatc aatcaagcga
     6721 gtaggtcttt cttgcccagg aggatctgtc gcaggatctg agtaagcaac ttcgattcct
     6781 tctaccttga tcgtcatatt ccttgctgca gcaaatcctg cataaataac cgattcatct
     6841 gaagcgtata atttttccca gtcatacatg atgtcagaag ccatttcttc tcctgtttca
     6901 ggatcgtaat aatatgactc taaaccagta ttcgtttttt tcatctctac gtccagttta
     6961 gtaatcgagt tcgtggccgg aaacaaattc ccatgaccgt ttccgaatat attataagaa
     7021 cctgtttctt tttccaagaa ccctgctgaa gtctctaaag gtgttgtttc cactgagaca
     7081 ctcccaggag ctggcgcagc ttgcggatct tcagatgata ttcctgtgat tgcccgtgtc
     7141 cccaatccaa gtcgcatcgt atatttgctg ccgctcgtgt tgaccacttc ttgtctatcc
     7201 gagtcccaga catattcgat ccgactgcct agtatggcaa atgaatttga ataaaatgcc
     7261 tgcccaaaca aatgattttt tggtactgcg tctcttacca ttaaagcaaa tccttcttgg
     7321 gcattcgtat aggtccaaga ttctatttcg atcgtcgccc gcatcctaaa gttgatgtct
     7381 ttggggattg cttgataata ataggtcatt ccatctgatc cgcttgactg gaattttccg
     7441 ccgttattcg tggaagagag ccgcacgccg tcttctaatg gttcaatggc atacctacta
     7501 gcacttgtgc tttctccgaa aacaacccct ttccactctt catttgcttc ttcagattgc
     7561 ttttttgtct tttcattttc tgattctttc atcataggat catttccttc ttccgtatcc
     7621 atatcttcac tagttcttgg tacttcctcc attgttgaca tcccttcaat gttcacgtgt
     7681 gcattttctt cgttcacaga ctgttgttcg ttagcagcaa cttcaacgac agaaattaaa
     7741 accggggtac tgagcataag gagattcatc attagcgcac tgtattttat acgttttttt
     7801 aacaagtgta ttcctcccgt aacaaattag aaagcgttta cttaaagtat tacctaattt
     7861 atttttactg tcaataaaaa acaaaattat aataagtgta ttttataaaa agaaaaaata
     7921 taacttaagt tgttgataat aaagagattt attaacgtaa aaagaataag tttttttaaa
     7981 ataacgtata aaaaagagca taaaacaaag tcgtttgttt tatgctcttc ttttattatt
     8041 ttgtgctttt tattttatca aatcactttg tttttttata aaatttctgc gcctagtgtg
     8101 gactcaaaat gtctcaatgc ccattcatgt ccaacgggat cgaaacttgc cacggcatct
     8161 ttgaaaatat ggagtttgta tcctagatta tacgcatcga ctgctgtatg caacacacag
     8221 atatctgtac atacgccggt caaataaata tctgtgatct ggcgttctcg caaacggatg
     8281 tctagatctg ttccactaaa agcggaataa tgccgtttgt ctatccaata aacattttcc
     8341 tcttgctcat agtcttggta taaacttttt aaagagccgt acaattcccg tccacccgtt
     8401 cctatcacat tatgcggagg aaataagcgg ttctccggat gaaaggaatc ttttggatca
     8461 tgcgcatcaa tggcaaaaac gacaaaatcc ttttgatcaa taaacttctt tgtatgagca
     8521 gtcagctttt tttcaattac ttgtcctgct gtcccagtag tcagctttcc atcctctgca
     8581 acaaaatcat atgtgtagtc aatcgagatc aatgcgttca tcttagtcct ccttatacga
     8641 gatgtttgac aaattcgatc accatttctg ctgatcgttt gcctgcgtct tcgataaatt
     8701 cgtcaaagct ttgtgtcgcc gagtgatctg ctgtgtcgct cattgcacgt acgatcaaaa
     8761 atgggatatt gaactgacga gctgtttgtc caacagcggc tccttccatt tcgcaagcta
     8821 atgcttcagg gaaattcgtt aagatttctt tgattttatc tggcgagtcg acaaatgtat
     8881 cacctgtaac gatcaatcct tcttttgcgt tcaaattcgt tgcttttgct gctttaacca
     8941 tttcactgcg caaatatgtg cttgcttcat aatatagagg cattcctggt aattgtcctg
     9001 gtttgtagcc aaatcctgtt gcatctgcat caaaataagc tactttgtct gatataacga
     9061 tgtcacctac ttgtaagcct tcaccgattc cgccagctga acctgtattg atcaccatgt
     9121 tcacgccgta ttgttggatc aacagacttg tggtaataga tgctaagact tttcctatac
     9181 cggaacgtac gacgatcact tcgtggttac ctaatgaacc tgaaataaat aaagcgcctg
     9241 cgcgttccca tgataatggt tcgcttaggt tctctctcaa aatttttact tcttcttcca
     9301 ttgcgccgat aataccgatt ttcatctttt taattccttc tttctatata aatgcgattg
     9361 ctaaaaaaac cacacataac attaggataa caataataag agatttcatc aaaaaagagt
     9421 tccatttggt cgtcttttcc cgttcgctga tccttgtttt cgtgattggt ttattctttt
     9481 tgctttcttt acgataaaag ctggcaatct ttttttcttc ttgctgataa gctgtttctg
     9541 cttttcgctg tttttttaat gactcgcttg cttgtgcttg ttgtcgcttg cgaagttcgc
     9601 ttcgcgtaat aagcggtcct ttggccataa gccgatccct ctactttcct ttggtcaata
     9661 gttcccagta ttgaatcgca taaatcgtct ttgcatcaca aatcaactga tctttcatcg
     9721 cttgttgcgc ctcttcaaga gttagatgat acaactccag tacttcgtct tcatcttgtg
     9781 ctaaaggatt ctcgaccttc tctacccctt gtgcatgata aatatgaagg acttcatttg
     9841 caaaacctgg tgaaagatac atcgaagtaa gatggctcag cgacttcgca cgatagccag
     9901 tttcttcttc taattctcta gctgctgtca tttcgggatt ttgtccttct cctggatcga
     9961 tttttccagc tggaatctcc aaaatcactt tttctaaagg ttttcgaaat tgtttgacaa
    10021 gaagcagacg atcttgttcg tcaaaagcaa tgatcccgac accaccagga tgaaatacca
    10081 gttctctctt cgaagttcct ccatctggca aacgaacttc gtctacagca acatcaataa
    10141 tttttccgct atagatttct ttgcgactga ttgttttttc ttcaaattgc tcattgttca
    10201 gcatatcttc tcctgctttc tttgtaatca taactgcttt tttttcattt ttcaattttt
    10261 ctcaaaaact ccttattagt ttaccataga taaaccttag attttctcta aatatagaaa
    10321 atccttcttt agtccgatac tctttctagt agtccgtatg ctaaaataag tatgtaatcg
    10381 aataacgaaa agaattcaat actcaacgat aaatgacatg gagggtttat tatgataaaa
    10441 cgaaattggc cgtggcttct agcattggtc ttgctatttc tctttgtccg aaacaatagt
    10501 ttttcttttg ttctaggact tttattgata ggtgtagttt tagttttgtg gggactcttt
    10561 ggttcgcgca ggaaaaaggg aaaacaagaa gaaattcctg cactgactga cgaacttgaa
    10621 actcactatg aaaaaagcgg tatgacagcg aatgaaattt cattttttcg acaaacaatg
    10681 aatcaaacaa aaaatgagat caatcaattg caaatgaata tgcagcagac agccaaactt
    10741 aaagcaatcg atctccgcca tgatccagtc aaagcagcga aagctttatt caaagaattg
    10801 gtgaaagaac caaaccgact gcatgaagca agtcagtttc tttatacgca tctgccgaat
    10861 ctagttgatt tgacaaataa gtataatgag atcaatgacc atgagatcaa aaacaaacag
    10921 acctatgaaa aactagaaga aagcgcgcag atcatcgacc agctgtcagc tttaatcgca
    10981 caagattatc aaaaattcgt tgctgaggat ctagatgata tcgacgtaga gatatctgtt
    11041 gcgaaacaga gcttaaagcg tgataataaa tatgaaccag aacaaaagga ttaactcttt
    11101 tgttcataaa agaagactcg gaacattcag atgatgtctg gctgtttcga gtactttttc
    11161 taaagtagag acattcatgt aagaatcgag aacagattca acaaggagga attttttatg
    11221 gatcataaac cgactgaaac aaaagatgtt acaccagtta acgatacact tgaagattta
    11281 ttgaacaacc cgttttcaac tccaacagat acgctgacca cttcacaaca agcggagatt
    11341 gatcagctcc aagagcaaca gacagctgct cgtttaattg acaaactacc ggcggaacgc
    11401 caagaacaag ctaaacagct agcagcaaaa atcgatgcta atgatgcaca gtctgtcatc
    11461 agctacggat cggctgccca agcaaaatta agtgaattct cacaatcgat gttgaatcat
    11521 gtgcaagcac aagatattgg tcctgtagga gattccttaa ctgaactgat gtatcgtctc
    11581 caagaagcca atcctgacga actgcgtgcc ggagaaggga acatcttcca gcgtgtcttc
    11641 gggaaggtca aacaatcgat ctatgaagtg acagcaaaat atcaaaagat tggtgcacaa
    11701 attgacaaga tttcagtgaa attagataaa gaaaaagacg gtctgctaaa agataatctg
    11761 atgttagaac agctttacca gaaaaacaaa gactactttg atgcactgaa tatttatatc
    11821 gctgctggag aattgaaaat ggaagaactt caaacaacga tcattccaga agcgatgaaa
    11881 agagcagaag aaactggcga tcagatggat gttcagattg ccaatgatta cacgcaattc
    11941 ctagatcgtc tggataaacg tacgcatgat cttcgcttag ctagacagat cacgatccag
    12001 caagcaccac aaatccgttt gatccaaaac acaaatcagg ccttggctga aaagatccaa
    12061 gcatctatcg ctacagccat tccgctgtgg aaaaaccaag tcgtgatcgc attgaccttg
    12121 cttcgtcaaa aagatgcagt aactgctcaa cgccaagtct ctgaaacaac aaacgactta
    12181 ttgaagaaaa attcagaaat gctgaaaatc tcagccatcg aaacagcgaa agaaaatgaa
    12241 cgtggaatcg tggatatcga gacccttcaa accactcaaa atgatttgat cgaaacaatc
    12301 caagagacat tgcgtatcca aaaagaagga aaagaaaaac gtcgtcatgc agaagtagag
    12361 cttggacaca tggaagaaga tctgaaacaa aaactgttgg atttaaccca atagatcacc
    12421 tttcgcttgt tactaaaaca aacgcccaac aacagtttat tacaactgtt gatgggcgtt
    12481 tttcttatct ttcttctaac ttctccattg gagtctattt ctcattcatc ttgctgtgtc
    12541 catagccata gccaaaataa atgatcagtc caataaccat tacgataccg aacacgatcc
    12601 atgtcacagt ttgcagacga agcatcaata caatacaagt caagatggag acgattggca
    12661 atagcggaac gaatggcact ttgaattctc cagcttttgg tttgccaaac atttttctca
    12721 acttcagtaa gcctagacta acaagcatca aataggcgag cgccatgata ttcgttagtt
    12781 ccgctaatac attcaatggg aaaaatccgg caaagaatac agctgccaca ccagcggcat
    12841 aagtcgcttt tttcggtgta cgatgctttt gatcgatttc tgtcataaac ttaggaagta
    12901 agccatcctt gctgacagca aacagtaaac gagcaagtga atacatcatc gagatcgtta
    12961 ccgtcagtag cgttaaaatt gcccctacag cgatcaccgc gcccactgtt ccatgtccta
    13021 catacctcat tgcaaaagcc actgggtcat tgactcctag ttcagtatac ggaacgattc
    13081 ctgtcaagat cagcgtcacg atgatgtaaa ggacagtagc aatcagtatc gaaccaatga
    13141 ttcctcttgg cacatctttt tgcgggtttt tgacttcctc ggctgccatt gcaacggcat
    13201 caaatcccaa gaaagcaaaa aacacaagag ctgcaccatc tagcacaccg gaaaaaccaa
    13261 aaggcatgaa tggctgccag ttatcaggtt taacgaaaaa tattcctaca atgataaaca
    13321 acgcgatgat accaaatttg acatatacca tggcattatt caaacgtaat gctttcttgg
    13381 cttcaagcga cacgatgtaa gtgacgaata tcactacgag caccgcaatc aagtcaatgt
    13441 atgtaccatt ttccggattg tacccgccag ttaatgcttg tggaagttcg gcccctaaac
    13501 ttttcaaaaa accttgcata tagccagacc atccggaagc aacagaggaa accgctagta
    13561 aaaattcccc gatcaacaac caccctgtca gccaggcagc aaattcacca aatacaacat
    13621 acagataggc atatggccca ccaatcactg gcactcgcga agcaaattct gcgaaactta
    13681 gtccagacaa aatgacaacg attgcagcta agacaaatga aagtgacagt gctggaccag
    13741 cattttgatt ggccgcaacc cctgtcacca caaatattcc cgtaccgatg atcgcaccaa
    13801 ttccaagcat gatcaaatca gacgttttta gttctttttt catctcactc gtatgattga
    13861 gattgatctc tttcttacga aacaaatcca ttgaagttcc tccccatttt aggttggaaa
    13921 acagcgatta aactgttttc atatacccta aaaggatacc acctaaaatg actaaaaggc
    13981 aaccaacaat aacataaatg agctcttttt tcgtttttct ttcgccaagg atgaaaatcc
    14041 cacctaaagt agagataatg atccccattt gtgaaagtga gaagccaaca gctaaaccaa
    14101 gtgatttgac tgttagaagc atgcagatgt ttcctaagcc ccataataat ccacagatca
    14161 tgtttttcca cacaaactga tcgactttaa ctttacggaa agcaaagaag cttgcgccta
    14221 agatcattcc gatactttga ggtaagataa ttgacatcgc atccaaattc gcccagttga
    14281 cgatgatggt atatacacca taaccaatcg tagagaagat caatgctcta aaacccttac
    14341 caaaatcaag cattttatta tctgtatcta tttttccttc gtcatcttgt cgggcagtca
    14401 aatacgcacc gcttacaaga agagctaaag caataatacc gtatacataa tctttcgttt
    14461 gtgtccattc atggaagaaa acggctcccg caatcgtatt caaaattaat tgaaaaccag
    14521 tagacaatgg tagccctaca gaaacaccca tgtacttcat tccatggaac tgtccatttt
    14581 gtccaacact ccaagctaag ccggataaaa atccgatcaa gaaaatccaa ggggtgatta
    14641 caggactaac aacgaaaaag acaactaaag aaaacagaaa tgcaccgatc gtcattccta
    14701 gtgtttgttg attggcatcg ccaccaattt taccacttac taaaccaata ctaccccatg
    14761 ccaacatagg gactaatgct attaaaattt ccataaccaa tctcctttta tttcacatga
    14821 taagaaactt atcagcgaaa acgttaacaa cacaagcact ttttttcgtt tctaacgatt
    14881 ttctaacgtt tttcattata aaaatttaag attgggaaaa ataaagtcat tgttgtccct
    14941 tttccttcag ttgattcagc cgtcaaatgg atacctaatt tttcggccag attttttgct
    15001 aaataaagtc ctaatcctgt tgaatgtgtt ttgctaagtc gaccatttct cccagtaaat
    15061 cccttatcaa agatccgttt caagtcttcc gctgggatac cgatccctga atctttaact
    15121 gaaaggctga ctccttgcgc ttccttttct atgctcacaa tgatgtttcc gtgatccggc
    15181 gtatatttga tggcattact aagcaattgg ttgaagataa aagcgatcca tttagcatcg
    15241 gtcaaaacag tatgatcctc accaacgatc tcgtagcgaa tatttttttg gataaaataa
    15301 tttgcctgac tgcgtatcac aggctgaatg attcctttta gtccatattc ttggatgagg
    15361 tagtcccgcg agaaactgtc gagtcttgca taatacaaaa cttgttctac gtattcatcg
    15421 atttttgata attcattttc tagtaatgta tacttttgat cgtcaatatc gaactcgatc
    15481 gaacgaacta gcaatttaga agcggaaagt ggcactttga tttcatggac ccatgagtca
    15541 atataatctt tttggtcttg ttgattactg attgctgatt gcattacttc ttggtgttca
    15601 cggatcagct gattgatata ttcttctacc agtttttctt cttctgtcct cccaccactc
    15661 aaataatttt gcaaagggga agttttttgt atgtcggcta attttcccca ccatcttctc
    15721 ttgctcaagt aaccacccag cagataaaaa atcagaaaga cgccctcaat caaagctaaa
    15781 taagcaattg tgctcaaatc aactttgatg tttggcgcca gccacatcac aaagatcgtc
    15841 aatgcaataa aaaacagcca tcccaagatc aatgtccact gatctttcaa atatttaaaa
    15901 aaattcatct taaccgctca tcctttcagg gaacgatata tccctgtcct acttttgttt
    15961 cgatatatcc ttggatacct gcctgttcga tttttcggcg taaccgattg atgttgactg
    16021 tcaatgtatt gtcatctaca aatcgttcgt catcccacag tgctcgcagc aatttttccc
    16081 gacttaagat ctttccattg tgcttcatca aaataaataa tagacgatat tcatttttgc
    16141 tcaaatcaat gatctctccg ttgatttcca tacttccgtt gtcaacattc aacgtaatgc
    16201 cattgtggct catcatttca ctagataatt ctgtgtagtt gtaagaacgg cgaagtaatg
    16261 cattgatttt tgcaatcagt acatcgattt ggaaatgttt tgtcacaaaa tcatctgccc
    16321 ccatgttcat agccatgatc atatccatat tcgtattcct gctggagata aaaatgatcg
    16381 gtactttcga tacttcacgt attttttgac accaataata gccatccaaa acgggaagat
    16441 taatatctag taatacaagc tgtggttctt ctctttcaaa gtctgagaac acttgattga
    16501 aatcggtcgt tccaaaggtt tcaaattgcc atttctcaag ttcctccatg atcaattgtc
    16561 gaatgatctc ttcgtcttcg actaccatga tttttgccat aaaaaatccc tccatctttc
    16621 ttcttgctac tacaatatca aagaagccct tgtgaaacaa gttggcttac ctggaaaatg
    16681 tcattttagc aaacgttaaa aaaaactgga aatatgcgat ttaatcgcgt ttgtctcttc
    16741 caagcagctt aaatttatta tgttattctt aataattggt ctattatctg ttatttttct
    16801 ttcgttgttt ttgtctttta aattttcgat aacaccaata accgaaaagc gttagcacta
    16861 gcaatatacc tagaaggagt ataagtaatc gataaagatg gtattttttt gtctgttcta
    16921 tctttgaaga gacttcttct gtaaagggaa ctcgtcttcc tgtcactagt aatcggtgag
    16981 tgttcacggt gtaaggtgta caagtgataa gtgtgactag atcctgcttt tctctaattg
    17041 ctaatgtatt tgtttttgtt ggaaggacaa caattttctc aacgacttca taagcgagca
    17101 tccgaccata cacttcaaga tagaatcggt catcttgctc catttcttca agatccgtaa
    17161 agagtttttt ctcagcaata ccgctatggg cggtaatcac ggaatgtgta ctctctcctc
    17221 cagttggaaa agaggtgcct tgcaggactg tcgctcctct atctaacaaa agatcattcg
    17281 tttcatcaaa taaaggcaag ctaacattga ttttcggaat atagatggct ccgatcatat
    17341 gttgttcgta atattctttt cctggatttt ggacatttct aaccgcttgc tcaaaaggat
    17401 ctttgacttg tcccattcct gggattactg atgtatgagc aagcgtctga tttttctttt
    17461 catgcactgc taattgttgc tcatgttttt cttctttatc gcgttcaagc tgcttttgat
    17521 aattctcgat ccttctttga tccaaataat tgctgagagc atctgctaaa aatggatatg
    17581 taaaaactgc agctccggca agaaacatca ctgccataaa taattgtaat aaaagactga
    17641 tttgggtttg aggtctatta cctattttct ttttcatttt cttcaccttc ttatttaaaa
    17701 aaactgcagc aacctttata aaaccgattg ctgcagctta tttttgttgc ttatacttca
    17761 gacttcattt tagatttacg gtaccagctg tacgctccaa tcatcaaact tattccgatt
    17821 agtaagaatg ccaagagccc gttcccacca gtagcaggaa gtgacccgcg tttcatattc
    17881 ggtactcttt ctggccctgg attttctact cctacgatct gaatttcttc attatagctt
    17941 ccataagaga tgataaattc agtaaaggct gcctcacctg ttggaagtac ataaccttct
    18001 ggcgccatgg tctctttcat ttgataggtt ccataatcta acccttgaac ctcaaagtat
    18061 ccatttccat ctgcgacaaa ttctgttgct tgtccgcttg ttgcccactc aattctatct
    18121 gctcctgatt cataaacagc taaaggcgac tcccctttat aaaatattgc gtattcagaa
    18181 cggtcttcat tccacaattc gaatcttgca tctttaagca attcactgct tgatgcatca
    18241 tatttttcaa actttcttcc acctgttgtg actttctctg gcggctctac tccttcttca
    18301 taatcaaatt catcatttcg tccgatttca ataaccgctg tgttgttatg gaaatcatcc
    18361 gcttctagtt ctgtggaaac cttcaaatta tacgtgatcg ttaaatctcc tccacgtgca
    18421 tctgctaatc tacctaaagt atctactttg gctactccat tttcttctgt caaaatcgtc
    18481 acgacaaacc cgttgcttac tacttctact atataatctt gtccttttgt taaaatcgtc
    18541 tcgccggctc gcacttctat ttcctgatca taataagtta gaccaactgt aggcgtatct
    18601 gtgattttgt aatttttgat aacagcgctt ccgttttcaa ggacagaatc gatgaaataa
    18661 ggaataggtg ctgtgatcgt gtaagaagcc gtttttcctc tttctagatc aatataagaa
    18721 atcgtttctt gttctccagc ttcattaata atcgttactt gtctaaggtc gctttcctct
    18781 acattcatct gtttatctgc ttctctgatt tcgtttttcg gatacaaatg cacatctgta
    18841 ttgtatgtgt ctttccatgt agaaccatcc cacacgccat ccaccatatc aggcatcatc
    18901 actggcatca taacaaccat tggagacgcg attctggaaa tatgagctgg cgaataagtt
    18961 tctaagaaaa gatagacttt atctctagaa gaaggtgctt cttccgtcac ttggacggta
    19021 gagaataccg cttctccagc ttcattcgtt gtttgtttgt caatacttgt cagtcgatat
    19081 tttaaaaacc aactttctgt gtgccgtttt tgaatccatt caatcgcttc ggcagaatta
    19141 agaccgtctt ctcgttccgg tgttaaagga ttatctcgaa tcaaatcgta atatacttcc
    19201 gttacgtcaa acaaatcaaa atcgactcct ggaatatttt cttcaccaaa atccgtagaa
    19261 accaatccgc tattcggtcg ttcttccgga acagatgaga aacctttttt gtgtaatgta
    19321 atcgtcactt cacttggttt ttgtgcgact tcttcggctt tgactagttt tccgttcccg
    19381 aaaatcccta ttcccgcagt aacgagtaac agtacactca ttcccgtgac aatcattttt
    19441 ttattcattt ttttctcccc gttctttttt ta
//
LOCUS       DACDXO010000051        19220 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E23605_2017
            SAMN12639989-rid9881463.denovo.052, whole genome shotgun sequence.
ACCESSION   DACDXO010000051 DACDXO010000000
VERSION     DACDXO010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639989
            Sequence Read Archive: SRR10023389
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741914.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:42:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,972
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 33 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 17 of 73
            Pseudo Genes (multiple problems)  :: 10 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19220
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639989-rid9881463.denovo.052"
                     /isolate="E23605_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            99..293
                     /locus_tag="IWM35_001996"
     CDS             99..293
                     /locus_tag="IWM35_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9919841.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
     gene            562..861
                     /locus_tag="IWM35_001997"
     CDS             562..861
                     /locus_tag="IWM35_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9919842.1"
                     /translation="MDYTVEFDSELLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKP
                     VRTRSAKGIYEMKVKINKKNYRIAFALKKNHVFIFYISSNLQKIVFDKEVSKKIK"
     gene            complement(958..1392)
                     /locus_tag="IWM35_001998"
     CDS             complement(958..1392)
                     /locus_tag="IWM35_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9919843.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(1624..2334)
                     /locus_tag="IWM35_001999"
     CDS             complement(1624..2334)
                     /locus_tag="IWM35_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HAP9919844.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(2324..3184)
                     /locus_tag="IWM35_002000"
     CDS             complement(2324..3184)
                     /locus_tag="IWM35_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HAP9919845.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(3189..3827)
                     /locus_tag="IWM35_002001"
     CDS             complement(3189..3827)
                     /locus_tag="IWM35_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HAP9919846.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(3842..4141)
                     /locus_tag="IWM35_002002"
     CDS             complement(3842..4141)
                     /locus_tag="IWM35_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP9919847.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            complement(4184..5668)
                     /locus_tag="IWM35_002003"
     CDS             complement(4184..5668)
                     /locus_tag="IWM35_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HAP9919848.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQALIFMGLILGCYWTVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGFLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            complement(5688..6149)
                     /locus_tag="IWM35_002004"
     CDS             complement(5688..6149)
                     /locus_tag="IWM35_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP9919849.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(6167..7234)
                     /gene="ulaG"
                     /locus_tag="IWM35_002005"
     CDS             complement(6167..7234)
                     /gene="ulaG"
                     /locus_tag="IWM35_002005"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HAP9919850.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            7452..8222
                     /locus_tag="IWM35_002006"
     CDS             7452..8222
                     /locus_tag="IWM35_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP9919851.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            complement(8495..9322)
                     /locus_tag="IWM35_002007"
     CDS             complement(8495..9322)
                     /locus_tag="IWM35_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9919852.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
     gene            9890..11125
                     /locus_tag="IWM35_002008"
     CDS             9890..11125
                     /locus_tag="IWM35_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HAP9919853.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
     gene            complement(12124..12384)
                     /locus_tag="IWM35_002009"
     CDS             complement(12124..12384)
                     /locus_tag="IWM35_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HAP9919854.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(12411..13712)
                     /locus_tag="IWM35_002010"
     CDS             complement(12411..13712)
                     /locus_tag="IWM35_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP9919855.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEEAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNAALLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPIIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
     gene            complement(14004..14462)
                     /locus_tag="IWM35_002011"
     CDS             complement(14004..14462)
                     /locus_tag="IWM35_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAP9919856.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
     gene            complement(14475..15800)
                     /locus_tag="IWM35_002012"
     CDS             complement(14475..15800)
                     /locus_tag="IWM35_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005227470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HAP9919857.1"
                     /translation="MKKQIITIAGGGSTYTPGIVQAILNNEKRLPLSEIRLYDIDEER
                     NMDMYLIVKFMLKRKGFSNIRIRATDDPKLAFTGCDFVFSQIRVGGLEMREKDEKIPL
                     KHGLVGQETCGLGGFAYGMRSIKGLLEIVDHIQDYAPKAWILNYTNPESIVSEAVRRK
                     YPNVKMINACDMTISIEETIAVNYGYDRKNWIVTYYGLNHFGWYTSIYDKELQREIMP
                     EIIEKLITKEMQVADFNIGDKTWQKTFQMMSVITKNFPSNIPNNYLEYYLYPDMVVEH
                     TDKEYTRANMVMDGREKNTKEMADKIRRGIVEEVLNFNFGEHGQYIVDIAISLLNDDR
                     RRFMLIVPNQGAIPNLRSDAVVEIPAYVGATGVEPITLRKPISDFHKGLMEAQVAVEK
                     LLVDAYFEGSYQKALQAFTLNQTVPNARVAKKILDEMIKVNKKYWPALK"
     gene            complement(15817..17229)
                     /locus_tag="IWM35_002013"
     CDS             complement(15817..17229)
                     /locus_tag="IWM35_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9919858.1"
                     /translation="MNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADDMLNIPVIGAA
                     GNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMDPEVNMGIFGG
                     IISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAVFLGFIWPLIQIGI
                     DNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSPSKEVFRGEMT
                     RFINGDPQAGLFLSVFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMGSGAGTSFIAN
                     ITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVDYLINFRIATN
                     AILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEETELGLASKNYE
                     YMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISGIIKPTEKNYQ
                     IIIGLEVTYVMAEFNKLLEE"
     gene            complement(17246..18010)
                     /locus_tag="IWM35_002014"
     CDS             complement(17246..18010)
                     /locus_tag="IWM35_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HAP9919859.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            complement(18114..18989)
                     /locus_tag="IWM35_002015"
     CDS             complement(18114..18989)
                     /locus_tag="IWM35_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP9919860.1"
                     /translation="MKESASCFVLSYRGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAVEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
BASE COUNT         6115 a         3951 c         2950 g         6204 t
ORIGIN      
        1 tgattctttt tatttatcat aaagatatag atacataatc attttcactt acatatattt
       61 gaatattaac taaaaacagc aagaaagaag aggatcaaat gtattatgta gaagtaaaaa
      121 caaaaggcgt caaaaacaaa caatatgtga aaggtatcag taacgaatat cctttacttg
      181 gttcatggaa agaagctgct cctttttcca aaccatgtgc cataaaaata aagaacgagc
      241 tagaaaaaga actgacttgt ggtaaagctg ttgtagatat catcgaaaag taaatccaat
      301 gtatagaaag gaagatcaca catgaaaagc gacgtacaaa ttgctaagat tatagaatta
      361 gaagaagaag acgcagtgtt gacaattgaa aaaaaataaa tgtttgtata ccatatgatt
      421 atttcaatat gtatcctgga attggagatc tcgtagagct atatcaagat gaatcaaata
      481 tacttgtcat tcatcatgaa atatagagac ttataaaatt gtttattttt ataatgtatc
      541 agtatagaaa ggaaataaaa catggattat actgttgaat ttgacagtga actacttaag
      601 caatttccac aagaagaaca tttcatgtat aaagaagcaa tttcccacaa agtgacagag
      661 tatttggagc ataatttttt tcgtataaag ccagtgcgta ctcgttcggc aaaaggaata
      721 tacgagatga aagtcaaaat taataaaaaa aattatcgca tagcatttgc tttaaaaaaa
      781 aatcatgtgt tcatttttta tattagttcg aatttgcaaa aaattgtatt tgataaagag
      841 gtgagtaaaa aaataaaata gctgagcatt ggaggataga ttgagtgata ctaagtctat
      901 cctcttgttt ttctaaagaa ctaagctaga attttttctt ttgtacgtta ttatatgtta
      961 tttgtcatta ccaaaaagca ccttttttct tctagtatct atgacataag aaataccacc
     1021 catttccttt acttgaacaa atcctactga ttcataaaat ttttttgttt tctcagtatc
     1081 atctgtcaat aattcgattt ggactggtcc ataactagtg atcttctcca aagcttcatt
     1141 caaaagcact ttgcctattc cttggcgctg atacctcgga tgaatgagga tatcttgaat
     1201 aaatgcgatc gtcgcaaaat cagtgatcca acgaacgatt ccgataatct tcccttcccc
     1261 attacgcttt atcagagatt cagattgttc aaatgctcta gctaaccttg cagtgtcttt
     1321 tgtgtaattc gtccaaccaa cgctcttata taattcagaa atctcatcga tcataaggga
     1381 atcaatattc atttttttct tcctttctta tacaattctc cctctaataa tacagttctt
     1441 tgactattta atctatcaca tataactctc ctgtaaagat tctctcgatt ccttaaaatt
     1501 cctctttcct tttagaatgc atactttttg ataaataaaa aggttgtgat aaattttctt
     1561 cacaacctct gtttctctct atcaagtgtt atcttttaat tttctaccct taccctagca
     1621 tctctattgc ccgtagtatg catactttcc atgtttgcga aaataatgtt tatctagtaa
     1681 atattgcggg atttcacata tattggggtt gatcagctcg ttttctttgg ccatcagaca
     1741 aatttcatct aagatcaaac tgttttctac tgctttcata ggtgaatccc cccatgtgaa
     1801 cggtccatgt ccatagacaa gtactccagg gacttcgttt ggatctaatt ttctttcctt
     1861 aaacgtctcg acaatcacat tccctgtatg aacttcgtaa gcttctctta cttcttcttt
     1921 cgttaattgg cgtgtacaag gaaccttacc ataaaaagca tcagcatgag tcgtgccata
     1981 agctggcaaa tcgcgtcctg cttgcgccca catgacggag tgcgtggaat gagtatgagt
     2041 aatagcagtt atatcttcaa aagtttgata caaaacaaca tgtgtgggga gatctgaaga
     2101 tggttttaag ctatcttctt ctattatatt tcccgacaaa tctgttacca ccatttgatc
     2161 tgcttgcatg cattcatatc tgacaccgct tggcttaatg acgatgactc ctagctcacg
     2221 gtccacttca ctcacattgc cccaagtcaa tttgactaat ccgtatcgcg gcaatgccag
     2281 atttgcttca aggacacgtt gcttcattgc tgcaattttt tcttcacaag acatcatagc
     2341 cagcctcctt caatttagaa cttaagtagt cttttgcctg ctggatttct cttcgaaagt
     2401 cttggctttt ttcactccac atttcaatca gaaatgttcc atcataatct agacgtttca
     2461 aatgtcttaa taatccaaga aaatcaacac aaccgctacc aaaaggaaca tcacgaaact
     2521 ggcctgaata ttcttttgtt acagggtaag tatcttttaa gtggatagca gtgatataat
     2581 cgatcccttt ctcaagttct cgagccggat tattttccgg ccatgcggac aaattgccta
     2641 ggtctggata tacttgtaga aaaggtgaat gaatctgttc tttgatttct aaaaattttg
     2701 aaatggagtt cataaatgga tcatccatga tttccacgga aagaatgatg ccatatttag
     2761 aagcttcctt tacgctttct tttattcctt cgataaagtc ttctctggaa ttcattgatt
     2821 tctcttcata ataaacatcg tatccagcta tttggattac tttgacgctt agattatgcg
     2881 ccaaatggat tgcttttttc attagttgtt tcgctgcttt tttcttttct ggatcagcag
     2941 aaccaaacgg aaatcgccga tgcgcactta aacaaatcga atggatacgt acaccggagc
     3001 tgaacatctc ttctctcagc tcagctatct tttcttcaga ccaatctaat cgtgctaacc
     3061 gctcatccgt ctcatcaatc gacatctcga taaaatcgaa gtccatttct tttacaaggg
     3121 aaaaacgttc tttccatgaa atatctttgg gtaacgcttt ttcataaatc ccaatttgag
     3181 ccatttcctt acccccagat acgaatgatc tcatctttga agttttgtgc ggccttcatc
     3241 ggatcatttg ctgctgtaat accacgacca gtgataaagg tatacacatc gatcccttca
     3301 aataagtgta atgtgtgtgg atttaatcca ccagttacag aaacattaaa gcctagttca
     3361 atcagttttt tgatcttcga taaatctttt tcaccccaag tttcaccaga taacagcgca
     3421 tcacggcttt gatggtagat tacctgccgg ataccgatgt tatgccaatc catggcttgt
     3481 tcaaaagtcc aattgccgta gagttctact tgcaattcac ctatttcttt ttgtgctgcc
     3541 tccatagttg gaatcgttgc acaacaaatg actgtcatcc agtctgctcc tgcttttgca
     3601 acattactcg ccactgttcc tccagcgtct gcacatttcg tatcagcgac tagtttttta
     3661 tctgggaaca tactgcgaat acagcgaatc gctttttgtc cttcctgtaa gcaaagaatt
     3721 gtgcccactt caataatatc tactacatca ccaactttca taacatctcc taatgcatgt
     3781 tctaagtcat tgtgatctaa cgcaatttgc aaattcggcc tgctcatctt agttccttcc
     3841 tttacttgtt tttttggtac cactttgttt cagcaaattt ttcactgatt tctttgactg
     3901 acatcacatt tttgataccg ataactgtaa ctcctttttt ctctgcctga gtaaacatat
     3961 tcaaaaaatt gactggcgta aaaactacat cgtattggcc agctgaactt tttccctctg
     4021 aaatggcaca atgatggatt ttggtaattg gtacgtttag ttctttcatt gctttttcca
     4081 cacttctcat catcatcaag cttgttcctg atccatttgc acacgatact aaaattctca
     4141 tcttaatccc tccattatga gtgttgtcta atcagatttt cttttatact ctctgctttt
     4201 ctgcatatgt gtcatagtct tctacaatca aaaaataacc atcaggattt ttccgatatt
     4261 gtaactgcgg aatagccaat aaaccaatga caactacggc aattccagca taacctagga
     4321 ctttcatgat tactgtaaac aatggccaag ctgttgccca gtcgaacatc ccgatataac
     4381 caccgtatgt tgacatccca atccatgtag cgaataatgc tgaaccgcct acttgtatga
     4441 ttcctgaaag aaacgggaac aggcaagcgg ctttgaatcc acctcggtta ttggcataaa
     4501 ctgctataac ggcattatca aagaacaacg gaatgaaacc ggctatgacg atcgttggtg
     4561 atttcaataa aatcagtaaa ccaatcatca aaaactgtcc caaagcacca aacaagaacc
     4621 caatcgttac cgcatttggc gaaccaaagc caaaagtagc agctacgtca attcctggaa
     4681 cagctcctgg taaaatcgtg tttgagattc cttggaaaga ttgagttagt tcatcgacga
     4741 atgtccgaac acctaattgc aagatcgcca gatataccgc aaaatttaaa gaagtcgtca
     4801 taatgtagaa taagaaactc tgcccttctt gcatgaattg cgcttgaatc aaataatctt
     4861 tgcctagtac aatcagaatg atcccaaaga aaaatagcat gagtaacgaa gtagcaacca
     4921 tattctcatt gaaaatcgac aagaatcctg gtaatttgat atcttcaatt tttttgtctt
     4981 gcttgattga tctatttttc ttttcatccc gctttttcat ccactcagct aattttgcaa
     5041 atataaagat tccgaacatt tgctgatgtg ccactgcaaa tccggcacct tctgtcaatt
     5101 cctgcgtgat tcctactgtt aagttagaag ctactgtcca ataacaacct aaaatcagtc
     5161 ccataaaaat caatgcttgt atcctaccta gatcaggaaa gcaaaacagt aaaatccaaa
     5221 aagcggtagc tgcttgttga acctgaacgt ttccggtagt gaaaacggaa cgcagtttcg
     5281 tatatttctg aaaacggact aataagatat ttaaaacaaa tgcaatcagt aataaaatca
     5341 tcacatctcc aaaggtacgg ccaaatgtct ccattaatcc agcatccaca gcattttgtc
     5401 caaaataagg atctgtaacc atggcatcca aattgaaccg ttcctttagt ccaaccaaga
     5461 ttggccgaaa attattgacc aaaccaccag agccaacagt caaaataaag taaccgaccg
     5521 ttgcctttaa gaatccagcg atgcattcat gaagcgggcg tttcagcaaa atatagccta
     5581 ataacacgat aaaaccaatc aggtatgctg gctgcgtcaa gatatttgtt gcaaaatacg
     5641 tccaaatgct taacaaaaat tcccccatct tttttctcct ctatcaatta attatattct
     5701 gccatcacaa cttggtaatc atcgatggag ttcgtagaga tgagacgctc aaccaatcca
     5761 tctgtcatta ataattcgga taatttttgg atattttcta gatgttcttc tggatctttt
     5821 gctgcgagtg taaaaaacaa actagccgtt ttctcctcat tctccccttc actttcaaaa
     5881 cggattggct gtttcaactt ggtaaatgaa atagctgttc caaaaacacc atcacttttt
     5941 tctgaagaat gcggcattgc tacttgaggt gcgatcacaa tataaggacc gtatttttct
     6001 acacaatgga tgatctcctc cacatacgtt tcatcaataa tttccttatc gatcaataca
     6061 agacagcttt cggcgatagc ttcacgccaa tccgtgattt ctttatctgg gtaatgaatc
     6121 aattgatggt caaaaaagta tttcagcaag aaactcccct tctttcttat aaaaacgacg
     6181 gaaacggtgt atcattttca atcgagaaga catcttcaaa tcctcgataa aaattaaact
     6241 caagatcatc tttgtttgtc ggatagatga atttcccgcc tacttgccag atataaggtt
     6301 taaatccata cttcaggcga tcctttttga atttccaaat ttcagtgatc tcttttggat
     6361 cagccataaa attagaccaa atatcgtagt ggacaggaat cactactttt gcattcaatg
     6421 actccgccat gcgcagcata tcaacagaag taactttgtc tgttatccct cgtgggtttt
     6481 caccataggc ccctaaacag acatcgattt tgtgttcatt accatgtttg gcaaataaat
     6541 tggagtaatg tgaatctcct gcatgataaa gattccctcc tgatgtttcg aataaatagt
     6601 ttactgcaat ttgatccatc tcctgaggca tttttccttt taatacctct tctggatctt
     6661 cacaagtgac caacgctgtc cgatcaaacg cttctaaagc tactacattg atgtctttga
     6721 ttttgactgc atcacctggc tttactgtaa tcgttttttc ttcaggaatt ccccattttt
     6781 gccacgtgcg aaccacttct tttggcccaa tgaatgttgc ttcaggacaa ttttgatgaa
     6841 ctgctgcggc tgtatggata tctagatgat cggaatggat atgtgtaacg attaaagcgt
     6901 ctacattttt tatcgcaaaa ggatcgatta cgaaaggctg agtacgtaaa ttaggctgca
     6961 tattttgaac gccactcata cgcatcatct gatggccttt tttcatcatg ccattcccat
     7021 gactacgttt tcccgtccca caccatagat cacataaaat attcgttcct tcatgacttt
     7081 tcagccaaat tcccgtacag cccagccacc acatagcaaa agttccagga tccactactt
     7141 ctgtttcgat ttcttcattc aagtatgtgc cccactcagg aaatgtttct actaaccact
     7201 tttcttttgt tacttcattg acagttttac tcatttgctt gctcccccca tatcgttgtt
     7261 ccaacttaca agtaaatatt aatgtgtatc agcatccatg aaaacccttt tatatgaccg
     7321 tttgtttttt atatgatttt ctttcagtaa tcaatgattg tttcagtata ataaagacag
     7381 atatgaatgt ttaaatgaac aactagtcaa ataaatccat ggatcaagat gtccctcagg
     7441 aggcaaaagt catgaaaaac tcacttgtca atattgaaaa acgtcaaaaa gaaattttgg
     7501 aactgcttca gcagactgaa caactatcaa ctttcgaatt agctgaatcc cttgatgtat
     7561 ctattagtac gatacgaaga gatttaaatt tattagaaga aaaaaacgag atcatacgca
     7621 attacggtta ttgttcttat aattacaaaa accagactga ttttgatcaa tctggtcctg
     7681 aaaggattaa acaagcgatt gccagagaag ctagtaaata cattaacgat tatgatacgc
     7741 tgtttatcaa ttctagctcg actgcgttga aaacactgga ttttcttcaa tctaaccatt
     7801 tgacagtcat tacaaacaac gtgaaaatca attactctcc gcacaaagat aattgtagtt
     7861 atattttaac tggtggagag atgcgctttc caaaagaagc actagtgggc gacatcgcat
     7921 taaatacgat ttcttcagtc aatgcagaca tttgtatcat tggctgtagc ggagtcgatg
     7981 tagtacatgg ggtcacaaca aagatattaa atgaatcaac gatcaacgaa atgatggtca
     8041 acaagacaat caaatgtaag attttagtcg ctgatcatcg aaaaatcgga ctgacatcaa
     8101 agttcaagat tgctgatatt tcagtctttg attatttgat cactgataaa ttttgttctg
     8161 aaaaagtatt agaggagatc agaaatacgg ggataaaaac cattcaagta gattcatttt
     8221 aagaaccagg acgtaaatat gtaaatagga ttatcaaaaa aatgaataac aaatttatcg
     8281 ataaatgaaa aacacgctac gctcaaattg ataaaggaat cccaagatgt atccatccaa
     8341 tcgataccat cataatctat tgcttctttt ataaccgtta tatgttgcct aaataggaat
     8401 caaacaatat cgaaaaaagg aggtatgaca aaagtcacac ctcctttttt cgaataaacg
     8461 gtattcaaat cagaacactg atttcacaac cactttattt caggtcgttt tcaatcacct
     8521 ttaccgcgta atcatttccg ttgaaacgcg ctattttttt tgcgtccata tatctttcta
     8581 aataatcatc ttctcctaat tgcttatggc aagaagcaat ttcataaagc aagtattcca
     8641 atgcataagt cgatttatgt ttcttattat actcaatacc cttctcacat aattggattg
     8701 ctttatcata atcctcgatt ttacggtaat aggtggcgat atcataatat acaatgcaga
     8761 ccatcagatt atcaaaatca gtctcactca atattttaag tgtacgctcc acataaactt
     8821 tcgcaaaatc taattctcct tttgacaagt agatcattgc cacgtttgca gctgcttgta
     8881 agtgatggat atctgtttcg gtcgtttgtt ccaacacttg attaaaataa aataatgctg
     8941 catctggttt tttcgcataa agatgatcaa gcagtccttg ataaaataaa atttgattta
     9001 aaagatcaga attacttact ttttcctgat cgatcgtttg taaaatttta taagattctt
     9061 ggtattttga tacagaattc aatgattgaa tctcatttat ttttttcctg attttgtctt
     9121 cttctgtttc tactaatata tcggagatct cgacattcag ctttttgcaa agcgcactta
     9181 agatattgat gcctggcgac atattcttgt tttcgatatt gctgacagtt gcttgttcac
     9241 aaatgccttc tgctaattgt acttgtgtta accccttttt ctttcggtat tcttttagta
     9301 atgctccatc tactttaatc aaaacaattt ccttcccttt aaaaaattat ttctccttta
     9361 tatcatacac aaaaaaaagt tctaaacaac caaaaatgtt tagaacagaa taaaaatttt
     9421 aattttaaaa taatattcaa attaaacaac tggcgtatct gctccttgat ttctttttgt
     9481 gttatctgaa taattatcgt ttacagttat aacacatagt gaaaattgtc atggcgagaa
     9541 agtctaagaa tatttttaaa cataactatt tctttcttac gtgtgtataa tgacgacagc
     9601 acacctatca attaatataa ttattttatt tttttatgtt ctcatgttta tttttaatga
     9661 cacaacttct cgtatttatc tggcacaaca tataaatcga tcaaaaaatt gttgcgtgtt
     9721 tttttgaaaa ttggagtttc aacttgatta caaaatttaa atttagaagt tgtgaaaaca
     9781 gcgagagaca gtttaatatt caaaatatac ataggctact cgaacgctgt gttttttagt
     9841 atactagtaa gaaacgaagg gatagactca ttccaataag gaggatttca tgaagacaat
     9901 tctttttgct cctgcagtat tcaatttagc tgagacaaca cgaatgattg aggttgccaa
     9961 agctatgaaa gaccgttatt actgtgtatt ctttggcttt tctaaaactt ttgctccttt
    10021 gatcacagaa agtggctttg agtatcattt actagcgccg atccttacga aaaaacaaga
    10081 agaacaaatc ataaaatttg accagttgcg tacacttaaa aaccctttta ccaaagaaat
    10141 ggttgcctct cgtatcacta gtgaacagag tctgattgct aagtttgagc ctcgactgat
    10201 tgtgacaggt tcgaatgtga caattttttt atcggcccgc aataaaaaga ttcctttagt
    10261 atatgttaaa ccctatgctt tgagtgaacc tttttttcaa tcagagcatg cgctgatgcc
    10321 ccacgagtta cgtttcttcg gccctttaag caatcccctt tggcactgga caaaaaagcg
    10381 tatagtaaat acccgatgga tccccaatgc cttcaaacac gctgcacaaa aagaaatgct
    10441 tcctttacca aaaacaagtt tacaaatgat ggatgctgat ctaaatctca tcacaacgcc
    10501 taaactattt ttggaagaag aacagctgcc ggaaaattat cgaatcgttg gtccaatttt
    10561 tgctaagctc agtacttcct tgcctgaaga agtgctaact tttatacagc gaaagcaaca
    10621 agaaagaaaa cgtattatct atttcgcaat gggtagttca ggcaatccgc gtatgttacg
    10681 caaaattttg ttcttcttac aaaaacaacc ggatctagcg gttattgcac ctattgacta
    10741 tctgcttgat gacattcaat ctgactcaga agatctttta ataacagaat atctgtctag
    10801 tcatcttttg catgaattca ttgatttttc ttttatccac ggcggggaag ggaccgtcca
    10861 aactgcctgt gaatccggca aaccgtttat cggtgtgggg atgcaatttg agcaaagctg
    10921 taatattgac tattgtgtaa aatacggaaa cgcaatcgcc ctcacaaaac cagtgactga
    10981 aagaaaactg cagattgctc tgcgcgaagt ttcttcaccc aagattcgtc agcaggcacg
    11041 cacgctgcaa gccgtttttc caataaatgg tccacaacaa gcaattcaag aaatcgaaac
    11101 cttcctgagc aaccacccaa tatagtttca agtagaaaaa gcttttttaa tagagactca
    11161 tcagtaattt cgctattcga gacccagaca gtgaaaatac aaaaaaagta aatagtggat
    11221 tgaaaagtta gagttactta aattacggtg tctaaaaaac acaaaatcat ccttaaaata
    11281 aaagatctga cgctatccaa atgagcatag gtagcgtctg ccatttattc ttagtcaaat
    11341 tctttgacac ctttctctgg ttggatcatc tcaacgtttc ttcaaaagaa agaccataat
    11401 aattaggcaa atacattttc ttaaaatgtg tcatatagat ctagaagcat cgctttgtag
    11461 agatttgtgc cgctagtccc ctcaacaatt actaaatcaa atgtaggatt taccacttta
    11521 tctttgataa tcaacctccc tcttctgttg agacaatgat ctcgcaatcc gatgcatact
    11581 cctatataaa ctgccagcaa tccccacata ttgagtagat catgtcagaa tgttctatgt
    11641 cgtcagcaac gatacactaa aagtcacgtt caccatgact gattgctatt cccagcacaa
    11701 ttatttctat acacgtttca tgatttgaat aaacactcac atcatacata ttaccattca
    11761 tacaccatac tgcagcccct actatatggt tgtaagctcc caaatctata tttgacttga
    11821 tcactttggt agtagatcta ctaattctaa atccattgct gataaaattt ccattttttc
    11881 ctccttcttt ctgccaaaac agttaaaaca ataataatct ttatgtaaaa aagggctgtg
    11941 acaaaagcct tgtcacagtc ccatattccg aataaacggt ggtcaaaagc ttgcatctgc
    12001 ggtaataagt tcaaccaaac gaaaaataag ccaacatttc acaaaaattt aagaagcaat
    12061 tttcgtaaat ttcttcttat ttcttgaagc taaccacttc tgttacaacc acttttagaa
    12121 catttaaagc ttcgatgtga caaaccatct atgctgctcg atcataaaat tgatagcaaa
    12181 aattccacaa agtacatatg tcccccatga aaacaaaaca ttcatatgcg gtacaatgat
    12241 ctttggaaga atcacgatca aaaatgatgc cagtgcccaa atccatcgat tgatttgaat
    12301 atgatatgta gctagcatat tcaatataag gtgactagcc gttaataaaa agataaaaac
    12361 aaaaaataaa atcagccaat acattgtttc ccaccttcta aaaaccaaac ttagacagtc
    12421 tggacttcgt tttctccacg actttgttca tctgacagca tctttttatc ataagctttt
    12481 acaaatggat aatagataat aaatgctatc aatgtgttga tcatgatcaa gataacaaca
    12541 cgccagtcat ttcctgctga aagataaccg ccgattggag ctggtaatgt ccatgttggc
    12601 aaaacagtaa atctattgac catttctaat ttgactgcaa aataactgat aatagtcaaa
    12661 actaaaggaa ctaagttgaa cgggatgaac atgtaaggat tcaacatcat aggagctcca
    12721 aaaattatgg gctcattgat attgaagata ccaggaataa tagaaaaacg accaacctct
    12781 ttcaagaatt ttgatttaca gaagaatata aacaaaacag ataccacgat cgttgctcct
    12841 gcaccaccaa ttgttaccgt ccattgataa aactgttcag gagcgatata gggtaattgg
    12901 gtggcagctg taccatctgc caatgccttt gcattctcat ccaacatgat cagccacatt
    12961 ggacgaacaa cagcccctac aatactcatg ccatggatac caaatgacca caacaaatgg
    13021 atcaaaaaca ttggtagtaa aacaccaagc aaattattcc ctaaaatatt ggttaaaggc
    13081 tcgaataacg ataataaggc agcgtttaca tcaaagcccg cgacgcttcg aatcagccaa
    13141 gtaattgtta aaatgactgc acctggaatc aatgcctcaa aactacgggc aacagcaggt
    13201 ggcacttgtt ctggcatttt gatagtaata tttcgatttt taaagaaacg atatacttcc
    13261 actgcaaaaa tcatagataa gatccccgca aacatcccag atgctcccaa agaactcata
    13321 ggtaagaccc agccaagagg tgcctcttca ggagttaaaa cgtttacagg aacttgtaac
    13381 ataaagaacg ttcctaaaga taatatacca cctgtcacac tatctaatcc atatgattta
    13441 gctagatccg aacccataac aaaagttgca taaatagcca tcaatcccat cgtcatccta
    13501 aagggtaaaa caatcgttgc cgaataaggg gcaataaatt cattccatcc aggaaacggt
    13561 aaattgataa agaccataaa aaatgtccca atcaaaatca atgacaatgt tgaaacaacc
    13621 ccattacgaa ttgctaataa atgacgctgt gtaccaattt ttgccattgg tgggacaagt
    13681 ttctcttcga cccatttcat cagcttttcc actaaaacca ctccttttca ataaaatatg
    13741 aaccattcat tgcatgacaa cgcaaagaat gttcataact aattatataa ttaagaaatt
    13801 ttatttcaat atattctctt aaataatcaa atttattttt aagaatcctg aaaataagtt
    13861 ttctaaatca taggagcaac aaaaaaattc gataattaac aaaaatcaaa atgattaaat
    13921 ctagtgattg aaacatatca tttcaaaaaa attttgtaaa ttcacccata aatatcaccc
    13981 tatttaactt ttttcatcgt acactaaaat tcaaaacaac tatctttaat ctttacttct
    14041 tggttttgtt ttaataaatt tccttcaaaa atgcctggag aaactacaat gatcaccgta
    14101 tctttttgtg cttgctccaa cttcttcaaa tctataatag ctaacagtgt cccttctttt
    14161 acctcctggt tactttcaac ccgtatctga aacggacttc cttcgagttc aactgtatct
    14221 aaacccatat ggatcagtat ttttctgcca gattcacttt cgatcgttat ggcatgtcgg
    14281 gtaggaaata tattatcgat tattccttta actggcgcat ggatatatcc atcatgattc
    14341 gttatagcgt atccaactcc tatcattttt gaagaaaaga cctcgtcttt tactgctgtt
    14401 aatggaataa tttgaccact agctacagct tttagaatgt ttcttgtttt aaagaaatgc
    14461 actttttatc acctctactt taatgctggc caatattttt tgtttacttt gatcatttca
    14521 tctagtattt tttttgcgac tctggcattt ggtacggttt gattcaatgt aaatgcttgt
    14581 aaagcttttt gataagaacc ttcaaagtaa gcatctacta gtaacttctc tactgcaact
    14641 tgtgcctcca tcaatccttt atgaaaatca ctgattggtt ttcttagtgt aattggctcc
    14701 acaccagttg caccaacata tgccggtatt tcaacaactg catctgaccg tagattcgga
    14761 atcgcccctt gattaggaac gattagcata aagcgacgac gatcatcatt caacaaggaa
    14821 atagctatat cgacgatata ttgtccatgc tccccaaaat taaagttcaa tacttcttcc
    14881 acaataccgc ggcgtatttt atcagccatt tctttggtat ttttctctcg tccatccatg
    14941 accatattag cacgtgtata ctctttgtca gtatgctcaa cgaccatatc aggatataag
    15001 taatactcta agtaattatt cgggatatta cttggaaaat ttttcgtgat gactgacatc
    15061 atttgaaagg ttttttgcca agttttatct cctatattaa aatcggcgac ttgcatttct
    15121 tttgttatta gtttttcaat aatctctggc ataatttctc gttgcaattc tttatcataa
    15181 atcgatgtat accagccaaa gtggtttaaa ccataatacg taacgatcca attcttacga
    15241 tcataaccgt aattcactgc aatcgtttct tcaattgaaa tagtcatatc acaggcatta
    15301 atcattttta cattcggata tttacgacga acagcttcag aaacgatcga ctctggattt
    15361 gtataattca aaatccatgc tttgggggca tagtcttgta tatgatcgac aatttccaat
    15421 aaacctttga ttgagcgcat cccataagca aatcctccga gtccacacgt ttcttgtcct
    15481 actaatccat gtttaagtgg aatcttttcg tccttttctc gcatctccaa tcctccaaca
    15541 cgaatttgtg agaaaacaaa atcgcatcct gtaaaagcaa gctttggatc atccgttgca
    15601 cgaatgcgaa tatttgaaaa accttttctt ttcagcataa atttaacaat caagtacata
    15661 tccatatttc tttcttcatc aatatcatat aaacgtattt cagataatgg caaccgtttt
    15721 tcattgttca aaatggcttg aacgattcct ggtgtatacg tactcccgcc tccagctata
    15781 gtgataatct gttttttcat catcatcttt tcctttctat tcttctaata atttattgaa
    15841 ttctgccata acataagtca cttctaagcc gatgataatt tgataatttt tttcagttgg
    15901 tttaatgatc ccgcttatcc ctaattgctt gattcgctgt tcctcgacca tggtgggatc
    15961 cactacctct acccgtaggc gtgtattaca actatagaca tccaggatat tggcttttcc
    16021 tccaaacgct tgtagcattt ttgttgccat atattcataa tttttacttg ctaaccctaa
    16081 ctctgtttct tctaaggtta cttcattgcc aaactctgcc ttagcttcac gtcctaatgt
    16141 ctgaatgttt ctcttattga tcaaataata aaaagtaacg taatacaagg caaaaaagaa
    16201 aattccgatt ggaagaatca aaatagcatt ggtagcgatt cgaaaattga tgagataatc
    16261 aacaatacat gctccgaatg taaaaccaat tcgtatattg aataagtaac agacaatccc
    16321 tgctaaaccg gcaaaaagtg catggatcac gtacaacata ggagaaagaa acatgaaact
    16381 aaattcaaca ggctcagtga tatttgcaat aaatgaagtt cctgcaccgc ttcccatgat
    16441 tcctttgacc atctctttat tttctttata agctgcttta tagatagcta gacatatagc
    16501 tggtatacca aacatcatta ctacaaaaaa aactgataaa aacagtccag cctgaggatc
    16561 tccgttgata aagcgtgtca tctctcctct gaaaacttcc ttgctaggag atgtatagct
    16621 tcctaaatcg taataaatat acgtattcaa cacatggtgc agtccaaagg ggatcagcaa
    16681 acgattcaaa aacataaata tccccacacc taatgctccc atatcaacga gcagttttgc
    16741 aaaattatca atcccaattt ggatgagcgg ccagataaag cccaaaaaaa cagctgcgat
    16801 tgtttgtaga atcatcacca tagtcaatgg gaatttttcg cccgcaaaat aagaaaaaac
    16861 tactggtaat ttttgttctt taaagcgatt atacgtccat gccgctacta aaccggaaat
    16921 aattccacca aaaatcccca tattcacttc tgggtccata atttctaagc cttttttcaa
    16981 ggtagcaatc gctaaaaaac cagtaagtgc aggtattcct ttgtccttat ttttgacaaa
    17041 accgattgca actcctatag caaataagat atccaagttt ccaaaaacgg catttccagc
    17101 tgcaccaatt actgggatgt tcagcatatc gtcagctgat aaacgcatga tcaatcccgc
    17161 taatggcatc gctgcaactg gaagtagcat cgctcttcca agcttctgca catttgattt
    17221 aacattcatt tttattcctc ctctattaaa tatcctcata tgttactaac tcaatcaaag
    17281 gatcattttt cactttttga gcaaatgctg aatgaattaa aaattcgact tggtgggtac
    17341 gaggatacac aaaacttgaa ccatttaaca attcctgatc caaatacgct gcgtgacaca
    17401 tgacttcgat cgttttatct tctctcaata tcatgagtaa acttgccaaa tattcttcca
    17461 cactcgtatt gatttgtcgt tttttttcat cagtttctcc tacgacatcg aaacaaggct
    17521 caaaataatc gacatgacga acttcatctt ttcgttcaaa gttgctccga accggcaaat
    17581 catatttttt tgctaatgta acaactacct cctgattttc atccaaagta tgagtatgat
    17641 gatgactgtc taaatgagtt ggtatgatcc ccgcatgata aattttttga atctgagcat
    17701 tccattcctg atacagttga tctttgtcaa tgttaaatgg atgctgataa taagataagg
    17761 atctgaactc tccgctttcg ataagagtat cgacagtttt gaggagtggt ttgccacacg
    17821 tcaatgttaa atggactcct acacctagcc taggcatttc ctttcttagt ttcacagcat
    17881 gctcgaaacc cggcatattt gccatcaatg tagttgatgt caaaattcct cgctgataag
    17941 catccataat cccataattc acaccatggc tatagccaaa atcatccgaa ttaaaaatca
    18001 cttttcccat ttcattctcc ttcttttttc tactaaaata ggcgcaacca aataatcata
    18061 gtccatttac tatgtgtcat ttggttgcgc ctatagatag ttacgccctt attttaatag
    18121 attcttgcta gatgaattgc caaatagatg atttcatttt cttgaatggc aagttgaaat
    18181 tgctgttcga tgatatcttt aatctttagt gctgtacaat acgctttgct atgtttaaat
    18241 gtttcagtaa aaaaaccaat attttctact ggcgtttctt cctgataaat caagcgttta
    18301 accaggaatt tgatatgaac cattaagcga tgataagtca gtgattcttt atcataaacg
    18361 tttgaatagg atttttctag atgggacaaa atcgtggtcg tcatttctac tatttttaag
    18421 ctctttaaac tattaggctc tcctagctca gcattaatga tatgcatagt gatgaatcct
    18481 acttcatttg accctaactc ggtttgatat cgatgattga ttcgctcgac cacttctact
    18541 gctatatcat attcatttgg ataaaggaat ctcatttctg gcaacataat tgctgtgaca
    18601 tctctatctt ctctttttcg ttgaatagca aaataaatat gatctgtcaa tgtaatatag
    18661 atatttgaat ctaatggaga ggagatccta gctgttatca gttcaatcac ttcttccgca
    18721 aaatccataa attcgattgg aatctgcgag actatctcac tgaatcgttt gatcttttct
    18781 ttagaatcta atacaaataa tttgtcagca tcctcaacat caaatggctt actcacttta
    18841 gcagaaaagc caatcccttt ccccatcgca attttttcta ttccatcgtc atcgatcaat
    18901 acagcattat gattcagctt tttcatcaca tacaccgtta attcacctcc tctatatgat
    18961 aaaacaaaac aacttgcaga ctccttcata catatttttg tctccaaaca atttttaaag
    19021 acaaaaaacg acctaagaaa gaaccctttc ttaggtcgtg cctaattgaa tagtagcacc
    19081 ctacataatt tacaaaaata gtttaacaca taataaaagc gttatcaata tcttttttac
    19141 tcgttttatg ataatacttg tatcgaattg atttggaggt aattacataa aaacaaaaaa
    19201 aatatagcaa ctctctataa
//
LOCUS       DACDXO010000052        19126 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E23605_2017
            SAMN12639989-rid9881463.denovo.053, whole genome shotgun sequence.
ACCESSION   DACDXO010000052 DACDXO010000000
VERSION     DACDXO010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639989
            Sequence Read Archive: SRR10023389
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19126)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19126)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741914.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:42:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,972
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 33 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 17 of 73
            Pseudo Genes (multiple problems)  :: 10 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19126
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639989-rid9881463.denovo.053"
                     /isolate="E23605_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..703)
                     /locus_tag="IWM35_002016"
     CDS             complement(<1..703)
                     /locus_tag="IWM35_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9919861.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFT"
     gene            complement(717..1641)
                     /locus_tag="IWM35_002017"
                     /pseudo
     CDS             complement(717..1641)
                     /locus_tag="IWM35_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            1758..2837
                     /locus_tag="IWM35_002018"
     CDS             1758..2837
                     /locus_tag="IWM35_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAP9919862.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            2869..3462
                     /locus_tag="IWM35_002019"
     CDS             2869..3462
                     /locus_tag="IWM35_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9919863.1"
                     /translation="MTKSLQTILESTKEIRVLGIGHCAKDALVLYETHRPDVLLTDIR
                     MPKQTGIDAAKEILAKFPKAILLLLTTFKDEEYIREAFSIGVKGYLIKQNLQAIIPSV
                     KSAYNGQAVFGNEIIETFTQMMKNKPTIDSHAYASFSERELAIIKEVAAGKNNKEIAD
                     ALYLSDGTVRNYISQLLEKLDLRDRTQLAIYFYQHLQ"
     gene            complement(3572..3799)
                     /locus_tag="IWM35_002020"
     CDS             complement(3572..3799)
                     /locus_tag="IWM35_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005229738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP9919864.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(3902..6490)
                     /locus_tag="IWM35_002021"
     CDS             complement(3902..6490)
                     /locus_tag="IWM35_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HAP9919865.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(6510..7169)
                     /locus_tag="IWM35_002022"
     CDS             complement(6510..7169)
                     /locus_tag="IWM35_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAP9919866.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVAQHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(7218..7721)
                     /gene="trmL"
                     /locus_tag="IWM35_002023"
     CDS             complement(7218..7721)
                     /gene="trmL"
                     /locus_tag="IWM35_002023"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HAP9919867.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(7942..8808)
                     /locus_tag="IWM35_002024"
     CDS             complement(7942..8808)
                     /locus_tag="IWM35_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAP9919868.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            8917..9558
                     /locus_tag="IWM35_002025"
     CDS             8917..9558
                     /locus_tag="IWM35_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HAP9919869.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(9620..10981)
                     /gene="mgtE"
                     /locus_tag="IWM35_002026"
     CDS             complement(9620..10981)
                     /gene="mgtE"
                     /locus_tag="IWM35_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HAP9919870.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(10999..11898)
                     /locus_tag="IWM35_002027"
     CDS             complement(10999..11898)
                     /locus_tag="IWM35_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAP9919871.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(11900..12697)
                     /locus_tag="IWM35_002028"
     CDS             complement(11900..12697)
                     /locus_tag="IWM35_002028"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HAP9919872.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(12675..13379)
                     /locus_tag="IWM35_002029"
     CDS             complement(12675..13379)
                     /locus_tag="IWM35_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HAP9919873.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            13510..14085
                     /locus_tag="IWM35_002030"
     CDS             13510..14085
                     /locus_tag="IWM35_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HAP9919874.1"
                     /translation="MTQMLEIEYKSMLTKQEYEIFIAHYQLTDKDFRVQTNIYFDTVD
                     EQLKKQNCGLRIRFVGHQAEYTLKTPAEKGRLETTDTFITEEAEAFIFEKRLPRSGAV
                     FQKLSDLNIDPASLIQTGKLTTKRAEFPIEEGLLAIDESWGDNLHDFELELEVGDASV
                     GKIAFINFLKRFSLPYRPAKNKIQRMLEAKN"
     gene            14203..14880
                     /locus_tag="IWM35_002031"
     CDS             14203..14880
                     /locus_tag="IWM35_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HAP9919875.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
     gene            complement(14959..16767)
                     /gene="pepF"
                     /locus_tag="IWM35_002032"
     CDS             complement(14959..16767)
                     /gene="pepF"
                     /locus_tag="IWM35_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HAP9919876.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSGAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
     gene            complement(16943..17941)
                     /locus_tag="IWM35_002033"
     CDS             complement(16943..17941)
                     /locus_tag="IWM35_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HAP9919877.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            complement(18167..18820)
                     /locus_tag="IWM35_002034"
     CDS             complement(18167..18820)
                     /locus_tag="IWM35_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HAP9919878.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
BASE COUNT         5525 a         4136 c         3324 g         6141 t
ORIGIN      
        1 acgtaaagct tgctgccaaa ttagcgattg aaactaacga ttgctttcta ggaatcaagg
       61 acaagcgaat accatttgac gattgatttg cttgctgatc ctgtacattg cggacccccc
      121 acatcgtccc gtagagaatc gccattccaa tcagagtgaa gaaataaaaa cttttgaaac
      181 tcatattacg acctgcatga acttcttgaa aagatacttc ttgttcaaaa acagtcatga
      241 tgggcgcgag atcaacgttc tccacttctg ccactttact cgaaattata ccaatacgct
      301 gtaaaaattg atccatgacg tttttcatca acgtctgact cattcctgct tgacccacat
      361 ggagcgaaac agatgaacca tcaatgagat aaaaaccagc caaatcatct gcatttagct
      421 tttcattcgc ttctacttcc gatagttcct gtacttcaaa taatggttta tcattgactt
      481 cgacttgttc taagactgtg gtaaaatttt ccgaaagcgc tgtttgatcc gctttgatta
      541 caattccagt attaatcgtt tcgagattgc caccttgatc aatctcgcca aaggccgcca
      601 taaacatcaa tcctaaaaga attggaaagg ccaatgtcca aaacaacatg gatttatttc
      661 gcagcagtat ttttgtgcga tatttataaa aatgccaaaa cattatttcc cctcctctag
      721 tctctcaaat ccttgccagt aatctctaaa aagacatcat tcaaggacgc ttctttggtt
      781 gctatttttc cgtaactgat cttttcttct tgtaagattg taagaatcgg caacaatact
      841 gtatttagtt cagtagttgt tacttggagc tgaccttttt tataagcgat tccatcagaa
      901 gctattgttc gctgacaaca cgttttctgg gagtaatggg atgtcgatcg tcaaacgatc
      961 cgccgtccgt accatatctt ttagagattc tttggttcct ttagcaatca cctgtccttg
     1021 atccatgatc acgatctggt cgcaaagaat ttctacttct tccatgtaat gtgttgtata
     1081 gacaatggtt gctccttgtc gattcagctc cttgatactt tctaaaattt tattccgact
     1141 ttgggggtct acagctactg tcggctcatc taaaaaaatt aaccgcggct tgtgggcaat
     1201 cccacatgcg atgttcaacc gacgtttcaa gccaccacta agttctcctg gacgaaacga
     1261 aacatatttt tctaatccca ctaattgaat cacctcttca atcaaggctt tccttttctc
     1321 tttttcggaa acatataaac cgcaaaaata atcgatattt tcttgcacac ttagttcgtc
     1381 aaagaccgca acgtcttgag gaacgacccc aatcattgcc ttgacagcat aattttcagg
     1441 agtcattgtt gtcccgaaca atttaatggt tcctttgtca taatttaaca aagaaagcat
     1501 acagttgatg gcagtcgatt tcccactgcc gtttggtcct aacagaccta agacttctcc
     1561 ttcttgtact tgcagatcaa aatgattcaa tgctacaaaa tctccatagc gtttgactaa
     1621 tttatcaatt tcgataatca tcatgaaaaa ctccttccaa atttcttgtt gatctgacta
     1681 taataaagtg caagaaaaga aactagtgac gattggctaa accacacatg acatttgtca
     1741 caatgaagga gtctgctatg cataaatctc ggctactcaa cgaaactagc tttctactgg
     1801 ttatctcatt gcttttgctg gtaagtcaag gattcgttgc tatgcaagtt atctatttat
     1861 tgatcgccgt tttattcact ggtgtcagtt atttactgcc tacaaagcca aattggattt
     1921 tccaagttat tctatttctt ttatgtgcct ttttacctga tcttgtcctg tttctgccag
     1981 cgacgatgcg agtgatcgcc cctgaagaaa gccaccttga ttacttgttt ttgttaggtt
     2041 ttttgttgat ttcgacggtt ggtaatcatc cccccatcaa ggtcgccttt gtcgtaattt
     2101 tgctgttttt ttccttttac ttacgaaaaa aagatcaacg aacgaccgat cttgagaaaa
     2161 agtacattgt ctccaaagat actcattggg aaaaacaaca agagttatcg gaacaaaata
     2221 aagcattggc cctgtcacaa gaaacacaat tagccttgca aattgtggaa gaacggaacc
     2281 gaattgcccg agacatccat gacaatgtcg gtcatctgct gtcaagtgcg attttacaaa
     2341 ttggtgcgct tgaaatgatc aatcaagaac caaaactaaa agaaccatta gaaaatttaa
     2401 gtgccaccgt tcacactggg atggatcgga ttcgtcaaag tgtccatgac ctccatgaga
     2461 catcgatctc ttttcaacac tccttagagt ttctgatcgc tgattttcct tcttctgtta
     2521 cgattgaagg aaacttgttt gaaggattga atgaaacgca gcaaaactgc ttcttaatgg
     2581 tcatcaaaga agccttaacc aacagtatca aacatagtca tgccaaaacc gttgttttac
     2641 attttcgaac gttgccggcc ttttatcgct tgcaaatcac gaatgatggc accagcccac
     2701 aaaaaaattc tgctcccggt attggtttga aaacgatgcg tcaacgtgtt caagaacaaa
     2761 agggacaagt ccatattttc caagaaaacc agcaatttca gttaacgatc attttaccaa
     2821 gggagggaca ccaatgataa cagtagtcat tattgagatg atccatttgt gacgaaatcg
     2881 ttacaaacca ttttagaaag cacgaaagag atccgtgtgt taggcattgg tcattgtgcc
     2941 aaggatgcgc tagtcttata cgaaacccat cgtcccgatg ttttgctgac ggacatccgc
     3001 atgcctaaac aaacgggcat cgatgctgcc aaagaaattt tggctaaatt tcccaaagcc
     3061 atccttttgc ttttgacgac attcaaagat gaagaataca tccgcgaagc attttccatc
     3121 ggtgtcaaag ggtatttaat caaacagaac ttgcaagcga ttattccttc tgtcaaatcc
     3181 gcttataatg gacaggccgt atttggtaat gaaatcatcg agactttcac tcaaatgatg
     3241 aagaataaac cgacgattga tagtcatgct tatgcttctt ttagtgaacg tgagcttgca
     3301 atcatcaaag aagtagctgc cgggaaaaac aataaagaaa ttgcagatgc cttgtacttg
     3361 agtgacggca ctgttcgcaa ttacatcagc caattactag aaaagctaga tttacgggat
     3421 cgcacgcaat tggctatcta tttttatcag catttgcaat aagtcttatc taacacaaaa
     3481 aaacctgaat gatgctcctt cgcatcattc aggttttttt cacatcttct attcaattgt
     3541 cccgcaaacc atttcctcac gattgaaaga atcaaggatt cgcagggctg ccaggattcg
     3601 ttggattgtt cggcgtgtta ggcggaaaaa taggcgcctt gtctaacgtt ttttctagtt
     3661 ttgattcttc acgatcgtct cgttctacat caggactttc attcaatttg aattcttttt
     3721 ccatctcatc taacttttta aagtcattgc cagattcttt tgctaatttc gctaatttat
     3781 cttcggtttc tttagccata aagtaacgcc ccccttttga tttcagctta gcatatcaca
     3841 ctgaaaaaac aaaagaaaac gctaaccaac gatcattttt agctatttct catctttaaa
     3901 atcaagcatt ttttgccggt ataaaatcgt agggagaact gttttccata ccaatcatcg
     3961 gatctactaa ctggttatcg atcacttctg ctgtaagaac gaaatcttta gaaacagcag
     4021 gttctacctc ttctttcttt tcttctccaa caaatgaaga aaacagatct ggttccactg
     4081 tttccattac ttggtttttc gtttcattcg tcccactgtc atcttctaca ggtagctttt
     4141 cttttacaac ccgtggctct tttgtatagt tcccatcttc aaatggatca tttccgacca
     4201 aagaatcttc ataagctgct agtttggtcc ggagagtgat attcatttta tcattatcca
     4261 tgatccttga tttcaacgta gtcatccggc ttgctgattc attcgctaca caagtgacat
     4321 aggcttgctc ttcccctaac aaaatcaaca gttccttact gcgagtcact gccgtgtaga
     4381 gcaaattacg ctgaagcatc cgctgatatt gatggaccat gggcaaaata accatcttga
     4441 attcactgcc ttgtgattta tggattgagc aacaataaga aagagtgatt ttattccatt
     4501 cgtttctttt ataagtgacc tcatttgcat caaactggat aactaattca tccactttat
     4561 cttcagaatc ttttgccaag atgatacccg tgatttctcc catatctcca ttgaatacat
     4621 tcaattcggg agtattgact aattggagga ctttgtctcc tatccgataa acggtgtcgt
     4681 tccaatgtac ttcctttttt ccaggtttcc ctggattgaa tatctcctgc atcattttat
     4741 ttaacgcatc gatccctgca ggtccccgat acattggtgc taatacttgg atatcttgtg
     4801 cacggaatcc cttatttttc gctttttcca caattttaga gatcaagggt tcaatttgat
     4861 aagcatcact tcggaaatag gaacgatctt tttgattctt tgtgaaatct tttggcagac
     4921 gtccttcttt gatctcatgg gcaagaggaa taatactaga accatcacct tgccgataaa
     4981 tctgattgag ttccattttg ggaatttgtt cgatttccaa aagatcatgg agtacttgac
     5041 caggaccgac agagggaagc tgatctttgt ctcctactaa gataacttgc atattcgtcg
     5101 gaatcgcttt gaataatgtg ttagccagcc atgtatcgac catagacatc tcatcaacaa
     5161 tcaaaagtcc gccttctaat tctttcgtac tgactgaagg tgttttttcc cgtccattca
     5221 gccccagaag acgatggatc gtactggcag gcagacctgt cgtttcgttc attcgtttag
     5281 ctgctcggcc agttggtgct gcaagtagaa taggaaaaat ttcttgggta tattttgaag
     5341 gatccaattc cagtccgttc aactctgcaa aaagattgac gattccgttg atcacagtag
     5401 ttttccccgt acctggtcct cctgttaaaa taaatagagg ggaacggatt gcttcagcga
     5461 tagcttgttc ttgcgaaggt ccatactgta tgccgaatat tttttcgata cgccgaatat
     5521 tcttctgcac ttctttttca ggataatgga tttctttttt acgactgaga aggcgctgga
     5581 tcgaattacc gatcccccat tcagaaaagt acaaactgtt ttcgtaaaga ttagtctctt
     5641 cttgttgtat ctttccttct tcaacaagtt ggataattac agtagcaacc tgatcaggct
     5701 ggatttcaat tgggcgactt gtttctaaaa tacggatcgt ttgctgaagc aatgtctccg
     5761 ccggtacgta tgtatctcct gtttgaactg catgctgaaa aatctgatgc aagatagctg
     5821 ctcgaattct tctcggtgaa tctgctgcaa taccgatttg ttcggctaga ttatctgcac
     5881 gtttgaaccc gattccttct atgtcttcga ctagctggta cggattttct tcgatgatat
     5941 ccaaagactc gttcttatag gcctggtaga tactgaaagc taactgacta ccgaacccgt
     6001 aacgattcag tcccatgatc acttgatcca tcccgtggtt tagccgaatc gtctcagcaa
     6061 gcatttctct ttttggcttc gtcaaaccgg agatttgttt taataccgaa ggatcagcaa
     6121 tgattttttc gatagcatct tctcccagca attctacaat tttttctgct gttttcttcc
     6181 cgattcctgg gaatttttcg ctggaaagat acgctacgat cccatttgca gttgttggct
     6241 tttcttgctc atatctttcg agtttaagct gccttccgta tttaggatga tccactaatt
     6301 ctccataaaa acgataagtc tcattttctt gaatatcgcc gaaacttccg gtgactacaa
     6361 tctctttttc acggtaatca gctgtggttt cactgatgtg taccaaaagc actttgtaaa
     6421 aattactagg attttgaaaa aaaatcgcgg caactgtacc aacgaaataa ggcatttctt
     6481 ctggctgcat cggaagccgc ctccttcttc tatagtaaag gttctggtgc tgtttcagtt
     6541 cctaaaaaat ctgtttggtt ccatactttc agttcatatg gcaataatct gcaaggctct
     6601 tcagtctcta aaatcgtcaa actactattg aacaatccac ccatttcacg caactcactt
     6661 agctcttttc ctgtcatcca ttgaatggct gcagttaaag aagctccatg tcctacgaat
     6721 aaaagtgggc catctttatg ttgggcaacc gcatctgcta caacagtttc gatacgtgtg
     6781 attgccgatt caatcgtttc tccgtgaaaa ggagaaggat catacagatc taaatgatta
     6841 cgcaaattcg gcagattatc cggatatttc tggtacattt catcgatcga ctgaccttct
     6901 aaagtaccca gccctagttc tcttagctta tctgtataaa aaatctctac cggacgagca
     6961 agctctttgt tgataccttc tgctgtatca cgtgctcgct ttgctgtact ggaataaatt
     7021 gctttaaaag gaacatattt tacctttttc ccaaatgctt tgatttcttc gtaactttgt
     7081 ggcaaaagcg gagaatcgcc attgctccct tgaaaacgtc tttcctgatt ccattgtgtc
     7141 ttcccatgtc gggtaaaata tagttccata cgcagcaaac tcctgtcttt cttttatttt
     7201 actctataat aaactgtcta atccagcttg tcattttcat agtgatgcgt caattctaaa
     7261 ttagggaatc cttgctggcg aagtgcttca tacactatca ttgcagctgt attcgataaa
     7321 ttcaatgagc ggacatgctc atcattcata gggatgcgta aacacttttc ttcgttttca
     7381 cgcataaatg tttctggcaa accggttgtt tcttttccaa ataaaaagaa gtgctcttct
     7441 ccatcagcaa aattttcgtc actatatact ttatgggcaa attttgagat caaatgtagt
     7501 ttacgattac ctaaaaatgt taaaaaagct tctaaattac tgtggtaagt aatatttaca
     7561 tcattccaat aatccaaccc tgcccgtttt agatgtttgt catcagttga aaaacctagt
     7621 ggctcgatta aatgaagcgg agtattcgtg gctgcacacg tacgggcaat atttccagtg
     7681 tttgcaggga ttaatggttc aaataaaacg atatgattca tcaggatcat tcctctcata
     7741 tttatgttcg tttctatttt ctgacaattt gatttttgca taaaaaaaaa cgtatcaact
     7801 cggtgtcagt cactgcgagt cgttacgtta gtgaagctct tctcactaac ttttatcaga
     7861 acaggaacct gataaaaacc aatgaaaaaa caacttgttg ctaatataac accctcaact
     7921 tttttttgca acctctactc attaaaaact gctagaaaaa cgttttctct tagttccttt
     7981 taataattct atgtcaattg taatcttctt caaaggattc ttatataagt ctgctttgat
     8041 tcgctcttct acctgccatt ctaaaggtga catggccaaa agatcttttt gattttcctc
     8101 tggtatctca aattcatagg ttacacgctg acgttcagtt aatttcattg cttcagcaaa
     8161 cttggctact acacgttcat tggaataact ctgtttttca ggttgattcg gataaaaagc
     8221 ctgacgtagt tcctttaaat aattttcttg tggcaccacc ttcaacactt ctccatcttc
     8281 tgagagtaca cgttggaatt cttggtaatg agaaggcgaa aagatattta aaaccgcgtt
     8341 gaatgtttga tcagcaaacg gcaaattggt caaatcagct acacaccaaa atgcttcggc
     8401 agattgttct gtagctgcgt agactccttc ttttgaaata tcaaatccaa cagcattttg
     8461 aatctttttc aattttgaaa gttcattcaa gaagctacct tctccgcagc caacatctaa
     8521 tagcgagact tcttcaggta aatgctgcgc aatagtctca ataacgggct gatacatgcc
     8581 agattggatc attctacgcc ttggaacgaa cattccttga tcgtattctg ttttaaccgc
     8641 atgggataaa aaataaagtg ttcctttttt ggaaaggtca aaacggtgat tatttttgca
     8701 tatcaagcca ttatcagact tttttacttc ttcttggcaa accggacagc ggaacagctg
     8761 atgattttct gccaaaaact ggcgtcctcg gtcaattttt tttaacatag ttccccctca
     8821 tttctgtttt atcttaccgt tagatgctca ctttggcaac tgctaaaaat atgatacaat
     8881 gcctagaaga aagtgaaaga taaaggagaa gattagatga tcaaagaatt ttgcgctgaa
     8941 aactacacat ctattcctct agcaattgct agaggagccg atcggatcga attgtgtgac
     9001 aacttagcgg tcggtggcac aaccccgagt actggtgtga tcgaagaaac gttaagttat
     9061 gctggggaaa aagatatccc cgtaatgacg attattcgtc ctagaggggg cgacttcgtc
     9121 tataacgata tcgaattgaa aatcatgcat actgatttga ttgaagcaaa gaaattaggg
     9181 acggatggcg tagtatttgg ttgtcttact ccttcaggct ggctagacga agaggcctta
     9241 gaactattga tcgaaacagc agaagggttg cagatcacct tccatatggc ttttgacagt
     9301 attcctgaag atcggcaatt cgaagcaatc gattggctgg tagaacatgg tgttgaccgt
     9361 atcttgactc atggcggtcc tgctgacaaa actattgtag aaaatttccc acggcttaaa
     9421 gaattgattg cctatacaga tggccgctta atcattttgc ctggtggtgg tgtcactgca
     9481 gagaatgcag cacatgtagc taaagaattg aacgtaagcg aagtacacgg aaccaaaatc
     9541 gtggatcttc agttataatc ttataaacaa aaaaacattt gcaacaggca taatgcttat
     9601 tgcaaatgtt tttgtttttt tacataaaat agcccataaa taaagaagca atattgaaat
     9661 aaatcagtac actcgtcaaa tcgctcaaag tcgtgataaa gggaccgctt gctactgctg
     9721 gatcaaatcc tagacgatcc attagcatcg ggattaaact acctgcaaga ttcgccactg
     9781 tgattgcaaa tagcatagcc atccctatga caaaccccaa gataaaatta tgtttccaaa
     9841 aacctacgat tagaaaaatc gtgatccccg taacagctcc tgtgacaacg ccggtcaaga
     9901 cttcagccaa aacaagtcga acaaatccat gatctttctc atcagaaatc gcaagtcgac
     9961 gaacagccac agctagcgac tgggttccag cattaccggc agttcctgta atcagcgaaa
    10021 tgaagactgc taaaatactc gcttcactta ccaagtcttc atagtgactg attaaagaag
    10081 cggtcgacat acccaaaaac aacagagtga tcagccaagg caaacgtcga gacgctgcct
    10141 ttactggatt ttcattaatt tcttccacat tgaccccggc aagtcctgaa taatcgctag
    10201 cagcttcatc atcaataacg tcgatgatat catcgactgt gacgattcct aacaaatgat
    10261 catcataatc ggtaacagga agagctaaga agtcataatc tctaaatgtt tgtgccacat
    10321 cttcttggtc gtcgcctaca tgaactgaaa gcactcgttc gctcatcaga tcagaaatca
    10381 ttgcgtcgtc atcattcacg attaggtcgc gcagagaaat aacccctact agctgacctt
    10441 cttgattaac gacatagatg taatagattg tttcagctac atctgcttcg ttcttcagca
    10501 cgtacatcgc agagcgaaca gtttgattgg ctacgatcga aacaaattca gttgtcatga
    10561 tcgcgccagc cgtttcatcc tcgtaatgga gaagttcctt gatttcactg gcatcgtctg
    10621 ccgacatcaa acttaaatat ttagcaattt gctttttatc cagcgtatta agcaagtcaa
    10681 cagcattatc tgtatacatc tcagaaagca tatcagctgc atagctagga cgcatttcat
    10741 ctaggtattc tttcatatgt tcgttgtctt cttcaatcac atcgaacata tccgctaatt
    10801 ctttaggcga cagataggaa tagatcagct gcctgttctc ttctgtaaga gattggtaga
    10861 attgcccctg ctcatagata tgcatctcta aaaaactgtt tctgaatgcc tgaatattat
    10921 tttcccttaa atcttgagtc aactgggcaa atctttcctc taattcttga ttttcatcca
    10981 aaccaatctc acctcgcctt attcttcaaa ccgtctcaca actgcctcca tgtctgcggc
    11041 taaaggctgc ttgaactcca gcaactcttc tgtaaatgga tggtaaaaac gcagatcata
    11101 acaatgaagg gcttgtcttt ttattcccag atccattcgc ccgccataca tatcatcacc
    11161 gagcagggca cagccaattg cggaaaaatg aactcttatt tgatgtgtcc ttcctgtatg
    11221 cagctggata tcaactaacg cgatttctgt tagtcgcttc tctatccagt actcagtctg
    11281 tgccgaacgt ccttcatcag agaccattcg cttcaatagc gaagaataat ctcgggcgat
    11341 cggcaaatcg atatgtccat gtgccgataa atctgaaagc cttccactga ctaaggcctg
    11401 atattttttc acaaacttct tttcacgaag ttgcatatct aattttgcgt gcgcaaatcc
    11461 atgctttgca aaaagcatca aaccagatgt atcccgatct agtcgcgtca ccacatggat
    11521 tacttggttt tcgtaacctt gttttttata ataagccttt acacgattag ccattgtccc
    11581 atttggatgg tattgggcag gaatagacga aacgcctgct ggtttattta ctaccagaac
    11641 gtgttcatct tcatacacga tatcgatcgg tgtttcatcc aaaagtacgg tttcatgttc
    11701 tccttcagca ggaatcacaa tggttacctt atcatcctta gctagagaaa acaagacatt
    11761 ttctacttga tcattaactt tgatttgacc gccttgaaat tttattttcg ctaacagacc
    11821 ctttgagatc cctttttctt ttaaaaaata ttttacttgc tgagaatgat ctttctcata
    11881 atcccatgta aactgcatat caatcctcac taataaaggc atctttcaca cgatgccaaa
    11941 aatgcatatg tcgataagaa gcaaaatgga tccgttcttc tgctattcga taataaattg
    12001 agcggatatt tgcttttgcg acattcaatt gatcaaccgt tacaagataa tcttctgtat
    12061 tttccaattt tatttctagc cattcatcat gggcaatcac gatcggagaa cccaaagtac
    12121 gaaacactcg attattcagt gaagcaatct ctgccaactg aaaggcattg atactaggat
    12181 ggatcacagc accgccgaca cttttattat atgctgtcga accagtaggc gtagaaatcg
    12241 tcaatccatc tcctctaaaa ctttcaaaca gctcgttttt gatgtataca tcggctacca
    12301 tcgtacgatt ggcccgttta atcgttgatt cattcagtgc taaaaaatgc tggtccggtg
    12361 tttcatccaa ataactgatc cgaacatcca gcaagggata actaacactt tgttcccggt
    12421 tcgttcgtaa gctgtctact agttcttcca gctcgtaatc ccgccaatcc gtgtaaaacc
    12481 ctaaatgacc tgtatgcacg cccaagaaac gcacatcatt cagacaatga ctgtagtgat
    12541 ggaacgccga caataatgtg ccgtctccgc cgactgagat gaccaattct ggctcatgat
    12601 catctcgttg aatccctgct tgttctaaaa gtgcgttcaa acgttctgtc acttctatag
    12661 tctttgcttc tttattatga acgatcgcta ctttcatccc ctgcctcctc tctttcgctt
    12721 tctttcttca aaagcgttcg atagtagaca tcatctgatt ttcctcttcc atgggagaat
    12781 aaccgctgtg cttcttggat ttcttcacgg atactggaca tctcttcatc caactgatag
    12841 gcagcttctg ccgcacgctg aagtcgttca ctcatttctt ctggaaaaac gccttgatat
    12901 ttataattca gcgaatgctc aatcgttgcc cagaaattca tcgcaagagt ccgaatctgg
    12961 atttccgcca aaattttctt ctcaccgctg atcagctgta caggatactc gatcaccaga
    13021 tgataagaac ggtagccact tgctttttta tttgtgatgt aatctcgttc ttggagaact
    13081 ttcatgtctt ttcttttacg gatgatctca acaacttgat ggatatcttc tacaaactga
    13141 cacatgatcc ttaacccggc aatatcagac atttcttctt ctaaacgtcc cattggaata
    13201 tgccgtaatt gtgattttgt tacaatacta tcgactggct tgactctccc agtgacaaat
    13261 tcaatcggga catgtcgatt ttgttcacgg aactgtttgc ggatcccccg cagtttcact
    13321 ttcaattctg atacagtttg ttcatatggt gctaaaaatg cttcccaatc tcgttccata
    13381 gctgatacct ctacttcatt aacattcatt gtttattcta ccatatatgc aaagtttttt
    13441 cgtactcttg atttatgcat tgaccaaaaa aagaaaatta ggcaaaatag acaaaaagga
    13501 gtcgatcaaa tgacacaaat gcttgaaatc gaatataaat ctatgctgac aaagcaagag
    13561 tacgagatat tcatcgctca ctatcaatta acagataaag atttccgtgt acagactaat
    13621 atttatttcg ataccgttga tgaacagctg aagaaacaaa attgtggcct acggatccgt
    13681 ttcgttggtc atcaagctga atatacatta aaaacacctg ctgaaaaagg gcgtctcgaa
    13741 acaacggata cctttataac agaagaagca gaagcattta tcttcgaaaa acgtctgcct
    13801 cgttcaggcg cagtttttca aaaactttct gatttgaata tcgatcctgc ttcattaatc
    13861 caaaccggaa agcttacaac aaaacgggct gaattcccta ttgaagaagg acttttagct
    13921 attgatgaaa gctggggaga caatctacac gattttgaac tggaactaga ggtaggggat
    13981 gcatctgtgg gaaaaattgc ttttataaat tttttaaaac gtttttcttt accttatcgt
    14041 cctgctaaga acaagatcca acgtatgctt gaagctaaaa attagaaacc tttcattttc
    14101 acaaggcact tgagttcatt tttttacttt tttctgttaa gttataggat gtgaaagaat
    14161 ctagtcacta gttgtataga atctattttg ggggagattc atatgatcga aatctattta
    14221 tttgttaatc ctttagggga atactgtttc gaaatggaac aacagttatt acaatttatc
    14281 gaagaagagt acgggacacc atcaaaagaa aagatgcaat ttcgctttct tcctttggtc
    14341 aatttgcaga caattgggga tgtcatgcaa caaaaaggaa tttctcagaa caatcttgag
    14401 gaacgtaatc acttattctc aatgacttat tctgcagcat tggactgcaa agcggcgcag
    14461 tttcaaggaa agaaaaaagg ccgccgtttt ttgatcaaat tgcaagaagc agtcggctgt
    14521 aatgacatac catattctca agaacttgct gaatccatat ttgaagaaat cggcggcgat
    14581 ttgaatatgt ttaaagaaga tcgtcagtct gacttcgtta aagaagtatt cttttctgat
    14641 caaaaaattg ctcgggaaat gggcatcaca aaacaccctt ctgcagtcgt ttacaattat
    14701 gcctgcgacc gtgattttgg tgttttagta gaagtaaaag gtacaagtag cctttggaga
    14761 atcacagagc tgtgtaaaac aacagataac tcatatcaag tttttaatgg tgaacattta
    14821 catacaaatg attctcagtt ccattcaaga actagccatt tacacttgct ttctaattaa
    14881 aaaaatacgt cgactccctt acaagagagc cgacgtattt ttttcgtttc tattcgagaa
    14941 aatcaaaaat agaaagcctt ataaacgatc gatcaattct tctaattcat ttagacgttg
    15001 ttcaaacatc gacattgcat cttcaatata tgcagcctgt gtcatatcaa ctcctgcttt
    15061 tttcattact tccacaggat aatcgctgtt acctgctttt aggtaagcca agtagttctc
    15121 tagtgcctct ggttcctggt ttagtatttt cttcgccagt gctgaagcag cagaaaagcc
    15181 agtcgagtat tggaaaacat agtaattata ataaaaatgc ggtatccgtg accattcgaa
    15241 tttaatttct gggtcttctt cgactgctgg accataatat tttgcattca atttaccata
    15301 actatcactt aggtattcac tagtcaatgg cacacctttt tcatcttccg tatgcatgaa
    15361 atgctcgaat tcagcgaatt gtgtctggcg gaaaactgtt cctttaaacc catccaagta
    15421 atggttgagt acataagccc gtacacgagg gtctttttct gtttccaata aatactccgt
    15481 taggatattt tcattcgttg tcgaagcaat ttctgctaaa aagatggagt agtcgccgta
    15541 cacataaggc tggttcgaac gagtgaaata actatgaaca ctatgtccca tttcgtggac
    15601 aagcgtaaat agctgatcca gcgtatcatg ccaattcaat aaaatatatg gatttgtgtc
    15661 atagcttccc gaagaataag caccgcttcg tttcccttta ttttcgacaa catcgatcca
    15721 acgttcagag aatgcttttt ctacgatggc catgtattct tcacccattg gtttcagtgc
    15781 ttctaaagct ttttcttttg cttcttcgta cgtaaaggta attggagctt cccctaagac
    15841 cggtgtataa agatcataca tgtgcagttt ttccacttct aataaacgtt tccgtaattc
    15901 catgtatcga tgtaacaaag gcaaatgctt gtttaccacg tctaccaaag tatcgtatac
    15961 actttcagga atatgattat tgctcaaaga cgcttctctg gcagagctgt aattacggac
    16021 tttcgcttta aaattatgtc cttttatatg tgtgcctaaa gtagaagcaa atgtatttct
    16081 aaattgttcg taaacactgt acaatccctt aaatgctgct tcgcgcaccc ttcgatccgt
    16141 gctttctagc aactgaccat acacgccatg agataattgg actatttcac cattttctcc
    16201 ttcaatcgtt ggaaaaacta gatctgcatt attcaaaaca gcaaatgtat cacttgaagc
    16261 atcaaagatt tcacctgctc cagcaagaag agattcctgc tcagcagata agacatgggc
    16321 tcgattatct actatttgct ggatataatg gcgatagact tccaattttg gttcttcttt
    16381 gaaatattgc caaatctggt catctgacaa ttgcaatatt tctggttcaa accaggaaac
    16441 ggcttcacta actttcgaaa acagactgct tgctcttgca taaagcgctt ggtaatctgt
    16501 atttccagtg tcttgatcgt ttttaaggtg cgcatataca taaatgactt cagtttggcg
    16561 ataaaccctc aatacgaatt caatcgcatt taaaaattga gaagcgcctt gatcaagtgt
    16621 tcctttgtgt ttttcagatt gttttaactc ttctgataat tccaaatatt tctcatcaaa
    16681 ctcttggtcg cttgaaaaga tcttggtcaa gtcccaagtt aaattttcag gcaattcttc
    16741 tcgttttggc aactgcttta cttccatgaa atccactcct tttcttccct tagctctatc
    16801 tatcaaaata gatcatagtc taacatccac atgtcacagc atctaacttt tctactcatt
    16861 catgtgtctg cttccatcaa ttttagcata actaaatctt gttttacata gaaaaccgat
    16921 tcctagccta tttttatact tactattttt tcaaagaaga caagtgtatg cattcgttaa
    16981 aaaactgctg atagaccatc tcttcatcaa tcataggaaa aagccattcc cttttatgtt
    17041 cttttatttg gcaaagccat gagttgaaat caggaacttt ctggttttcc aaggcttctt
    17101 cgtacaacat tcgataaaga aaaacctgtt cttgtaaata aaagaaaaac cgactttttt
    17161 gatagatcca cgatgataga tgcaaaagat gtttatgacg caagtagcat tgttcttgta
    17221 tggatactgt attcttttgc ttgtgaataa gttggcgtgt cagccatttc ttcccatctt
    17281 cactcacttt ttctattttt ttcatttggt tcattttaat ttcgttacta gtgaatattt
    17341 ctttaaggtc cctactagaa aaagtccagc agctactgca aaaggaaata tgccctgata
    17401 cagtctctgt aatatgatga tacaaataaa gcttttgttt cttgagatca gctttccagc
    17461 aatgcactcc tctttttcta ttatagtagc agcaactttt ttctatcaat gagaattgcg
    17521 attgtccaag aaaagaatta ccaagtatcc accatacttg gtaactgtta ttcaaataat
    17581 tttgggttct ttcaataaat ctctggtgag acagtctaga acattggatt tctattgcta
    17641 gattcgccaa caaaatatcc ggcctttgcc gcaacctcgg caaataggct tccaaaaata
    17701 ctggttctac tgactgtccc agccaatcca tgaagcattc ctttaactgt aagtgctctt
    17761 ttgattctct ttcactaaag accgaacagt tatctgattt ttgatgggca aaatgagcac
    17821 attttttctt tccctttttc aaaatcactc tttttcgaca gccacgacaa taatatttct
    17881 ttcctttttc tgcatgttta gcaaaacagc acttattata ttcattcatt gcaagcagca
    17941 tttttctccc tctgaactta tttacgtcta agaatttatt tacacgaaaa aagagggcgt
    18001 gaaaaagcta ttctgcatca agtaataaga acagctaaac aaaaatagtt cccaatattt
    18061 ttcgtttggt tgggcttgtt accgcagatg caagcttttg aacacattta tacgaaaaaa
    18121 agactgcgac acaatttttg tcacagtctc ttactataat gctgatttat acaaaataaa
    18181 atttcgttcg ctcgatagca ttacgcgcca tcagacattg tccatattca ttcaatgttt
    18241 ctggtgtacg atcgctcttt tcagcaaact ccaaaattcg agcaatctga ttctctacat
    18301 ccgcttggtt gagattttcc atccagaagt gaacgactag gtaatagcgc tcttcgaata
    18361 aatacaaatc agtccaagca gactctagtt cgacatctgt tgcaagttgg atcacttgtt
    18421 caaaatcttc aaaagcaaaa gttgcttgtc tttcctgttc ttctagggcg cttccagata
    18481 attcttcctc cggatcgctg ccaataatct gctgacgaag aaagtcactc acatcttctg
    18541 aatcaacagt agaatcttct gattgagaaa tttcttcatc cggaagattt ttactgataa
    18601 acagttctaa tccgtctcct tttggcaaaa cttggaaagt gacagcttcg cttcctcgga
    18661 actcctcttc gatatcaact tcttccaaaa tactataaaa gaaattttcg atttctttgt
    18721 gattgccaag taaatccaaa aatgaaacgc cccgttcctc taaatcttca tgaccaatta
    18781 gtacacggat cgtattttca ttaatatgtt ccatttccat tttgctacac ctcactttgc
    18841 cacaatgttt aatatagatt cattgtagcg aaatcagctt aaatgtaaag aaaaaaaatc
    18901 tcgctgttaa gcgaatttaa taaaaccagc ggatttttca acctttcaag ctatcttatt
    18961 taactataac atttttggca gacgaatact atatagccga cttttcagct aattttccct
    19021 ctctattttt cgtgcaatca tcatcttcta ctaccttttt atacatctaa tcaagaatac
    19081 tttttgtaaa aacaaaaaaa cttggaaaaa ctatctttgt aatagt
//
LOCUS       DACDXO010000053        18739 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E23605_2017
            SAMN12639989-rid9881463.denovo.054, whole genome shotgun sequence.
ACCESSION   DACDXO010000053 DACDXO010000000
VERSION     DACDXO010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639989
            Sequence Read Archive: SRR10023389
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18739)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18739)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741914.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:42:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,972
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 33 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 17 of 73
            Pseudo Genes (multiple problems)  :: 10 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18739
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639989-rid9881463.denovo.054"
                     /isolate="E23605_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            255..530
                     /locus_tag="IWM35_002035"
     CDS             255..530
                     /locus_tag="IWM35_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9919879.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            543..2615
                     /locus_tag="IWM35_002036"
     CDS             543..2615
                     /locus_tag="IWM35_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HAP9919880.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKRVQ"
     gene            2780..4666
                     /locus_tag="IWM35_002037"
     CDS             2780..4666
                     /locus_tag="IWM35_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAP9919881.1"
                     /translation="MLKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKT
                     SLLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVR
                     AYEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIS
                     TLSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDR
                     YFLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMR
                     AGVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRIND
                     QTLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQ
                     ENEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEK
                     RRLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVA
                     DKLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKE
                     WETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSE
                     WVED"
     gene            4678..5625
                     /locus_tag="IWM35_002038"
     CDS             4678..5625
                     /locus_tag="IWM35_002038"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HAP9919882.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            5645..6172
                     /locus_tag="IWM35_002039"
     CDS             5645..6172
                     /locus_tag="IWM35_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAP9919883.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(6235..6888)
                     /locus_tag="IWM35_002040"
     CDS             complement(6235..6888)
                     /locus_tag="IWM35_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HAP9919884.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            7020..7862
                     /locus_tag="IWM35_002041"
     CDS             7020..7862
                     /locus_tag="IWM35_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAP9919885.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            8020..8916
                     /locus_tag="IWM35_002042"
     CDS             8020..8916
                     /locus_tag="IWM35_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAP9919886.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            8919..9632
                     /locus_tag="IWM35_002043"
     CDS             8919..9632
                     /locus_tag="IWM35_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HAP9919887.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            9652..10170
                     /gene="msrA"
                     /locus_tag="IWM35_002044"
     CDS             9652..10170
                     /gene="msrA"
                     /locus_tag="IWM35_002044"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAP9919888.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            10167..10394
                     /locus_tag="IWM35_002045"
     CDS             10167..10394
                     /locus_tag="IWM35_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HAP9919889.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            10546..11097
                     /gene="lepB"
                     /locus_tag="IWM35_002046"
     CDS             10546..11097
                     /gene="lepB"
                     /locus_tag="IWM35_002046"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAP9919890.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(11180..11722)
                     /locus_tag="IWM35_002047"
     CDS             complement(11180..11722)
                     /locus_tag="IWM35_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HAP9919891.1"
                     /translation="MNTLIAKDVFRLPEMNARLLSTSALLLALDVILYKIAIGPAYFQ
                     ITFGFLSMALMGYLYGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLF
                     LYHKKINLPSVIAAQLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQI
                     MVVYSFIKTLEARKVFKRAV"
     gene            complement(11959..12357)
                     /locus_tag="IWM35_002048"
     CDS             complement(11959..12357)
                     /locus_tag="IWM35_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAP9919892.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            12617..13477
                     /gene="ylqF"
                     /locus_tag="IWM35_002049"
     CDS             12617..13477
                     /gene="ylqF"
                     /locus_tag="IWM35_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HAP9919893.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            13470..14237
                     /locus_tag="IWM35_002050"
     CDS             13470..14237
                     /locus_tag="IWM35_002050"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HAP9919894.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            14293..15150
                     /gene="dprA"
                     /locus_tag="IWM35_002051"
     CDS             14293..15150
                     /gene="dprA"
                     /locus_tag="IWM35_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HAP9919895.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            15272..17350
                     /gene="topA"
                     /locus_tag="IWM35_002052"
     CDS             15272..17350
                     /gene="topA"
                     /locus_tag="IWM35_002052"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HAP9919896.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            17319..18695
                     /gene="trmFO"
                     /locus_tag="IWM35_002053"
     CDS             17319..18695
                     /gene="trmFO"
                     /locus_tag="IWM35_002053"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HAP9919897.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
BASE COUNT         6518 a         3231 c         3899 g         5091 t
ORIGIN      
        1 tttgtcgcag ccccattttt ttgttgtgat aaaagaacaa gaaaggcagg aatgggaaag
       61 taaacatcct tttgtcagaa acttttatct ttcttaagtt ttacttgata tctgatacta
      121 tttggaatag aatgaggcgt tgaaaaattt atggaaattt ctaactagca ataaacgatg
      181 aaacaatgaa atatttttca tccaaaaaga actttgctat aatgatggaa gtttgtgtat
      241 aggagatctg gaaaatgaga gagagttttg aacaacaaaa aaaactgttg catgatcgct
      301 atggcgccct ttcgatggat gaccgccgtc aaatactttg taagttgcgt aaaagaaata
      361 tattgatgta tcgtcagttg gaacgtctga aacatgatct gcttcgtttg gagtcaaaac
      421 gtgtacagtg tgaattagag gggaaccaaa cacaggttga agtagtcgaa accaaaattt
      481 taaagaaaaa agaacaattt ttaaaaatgc ttacacaaaa taaaaaataa aggggatgga
      541 cgatggaatt tgctgagtta gttattgtat ttgcagtaac gatcacctta tcgaacatag
      601 ctagccgcat tttgccaatg attccagctc cactgatcca aattttcctt ggagtggtat
      661 taggtctgac agagtgggga caatcaatcg attttgaacc tgaattgttc ttagttatga
      721 ttattgcacc attgttattt agagaaggag aaaaagcaga tatttcttcg attctcaaaa
      781 atttcgggac aattttgttc ttggcatttg gaggagtcat tttgaccctt gtcggggtag
      841 gtgcgacgtt atcattcctg ttgccaagtg tcccacttgc tgcctgcttt gcttttggag
      901 cagcgttagg tccgacagat gcggtagcag tcagttctct ttctggacga gtgaatatcc
      961 ctaaaaaggc gatgcatatt ttggaaggag aaggtctttt gaatgatgca tctggtgtta
     1021 ctgccttcca gtttgctctt ggtgcgctga tcaccggatc tttttcagca gtaaacgcgg
     1081 ggatgagtct agtagtttct agtattgggg gagctttgat cggctttttg cttgtctggt
     1141 tcaaacaaaa aataattcat ttgatcgaaa aagcatcagc acaagatgtc acagggtatc
     1201 tgctgatcga acttttgctt ccttttctag cctatgtatt ggcagaggca tttgatgtat
     1261 ctggaatcat tgctgcagtg gcagcaggta ttctccaagc ttctggtttt aggaaaatct
     1321 ctgtgtttga tgcagaattg tcgagcttgt cccacagtac atggacaact attgctttta
     1381 cactgaatgc attagtattt atatttttag gaatcgaatt gacgcaagtg ttctcgccag
     1441 tatggggaga cggtttatat ccaaacggtc ttttattagc gattatcgta ttaatctctg
     1501 tcatgctttt tgtgatcaga ttcatttcta tcagtctttt ttatgtattc aaagatggca
     1561 gtaaaaagtt caaaaaacag ttgaacgaga tcttgatctt aacatttggc ggtgtaaagg
     1621 gaacagtcag tttggctacg atttttattt tgccattttc aatcaataat atgatgtttt
     1681 accagcggtc tttgcttttg tttctgacgg cgggtgtcat cttagtgact ttggtcatag
     1741 ggatcattgt cttgcctatg ctgacggaaa cagaggaagc gaaaagcacg gatttgaatg
     1801 ctttgatgat tttagaagag gtcgttgaag ttttgcgaaa agaaataaag gaaattgatc
     1861 gtaatacaaa agaattttta gccactgaag cagtgataga aaattatcaa gaacggatac
     1921 gtgatctgta cttggaagat ttaaccgatg atgaaaagca agaagtacaa gaaatacaag
     1981 cactgatttt gtcgatcgaa cgggatggat tggatgaaag ttatcgttcg ggcaagcttt
     2041 cttctaatgg ttatcgtttt tactcccgtt ttctttctcg attcgaacat tctatcacaa
     2101 gccagatttt gtcttttatc ggcttttggt tcattgttgt ccgtaggtta atgcgaatcg
     2161 ttctccatcc gaaaatgttt tggcaaagaa gatatgctga tcgtcagaca tttattagtg
     2221 atgaagatat ccaagaaatc agagagacat atcaaaaaaa tactcagttg attattgaaa
     2281 gtttagataa cttgactgat atttatgatg aaacgatggt tcaatttttt atccagcaac
     2341 gcaaacttga aggaataaaa atgaactcgg ggaatttgat ttcttcgtgg atgatccagc
     2401 aagatagtct gtttacgaaa aaaatgctaa gaggttatta tcttgaaaga aaaatcattg
     2461 atgaatacga agttgctgaa aaaatcacga cgttttctgc gaacagctat cgcaggaata
     2521 tcaatttgtt agaatcttat acgatgaaca aaccatctga taacttttct ttccgatttg
     2581 cgtttcgagc aaagtcaaaa aaacgagtcc aataaaataa aaaggtaaaa attttcaagt
     2641 tttataaaaa acaaaacata tcagaacagg atcgaatcgg tcctgttctt tttttgagaa
     2701 cacaaaaaga acaaatacca aaaaaatttt cctgaagttc actattttta tgatacaata
     2761 tccaagataa ggagggaaca tgttgaaaga attaaaagta actgacctca aaaaaactta
     2821 tggtgaaaaa gatttgtttg accagatttc ttttttggtc cacgaaaaag accgtatcgg
     2881 gttgattggg acgaacggga cgggtaaaac aagtctctta aatattattg ccggtatcga
     2941 tagcagcgac ggtgatcggc aaacagtgtt ttatccaact gattaccgta ttggttatct
     3001 ttctcaagca gctgaatttt ccgatgaatt aactgttctc caagcagtct ttcaaggaaa
     3061 cagccccttg atccaaactg ttcgagcata tgaggaagca ttgatcgaac taggggaaaa
     3121 cggagaagac cctgatgtgc aaaaacgtta tgctaaagca gaagaacaaa tgaataaaga
     3181 agatgcttgg actacagata ccaacgcaaa gattattttg caaaaattag gaatcagtga
     3241 attagataaa aaaatcagta ctttatcagg tggtcagaaa aaacgagtta gtctggcgca
     3301 agtgttgatt gatgaacccg atttactatt actagacgaa ccaacaaacc atttagatta
     3361 tgaagcgatc gagtggttgg aaaactattt gaaacaatac cgtggcgcac tattgatggt
     3421 tacccacgat cgttattttc tagatagagt agcaaaccga atcttcgaac tttcatttgg
     3481 aaaattatac gagtataaag gaaattatga agcttacgtc ttagaaaaag cagaacgtga
     3541 tcgtgtagaa gtagaacaag aagaaaagcg caagcggctt tataaacagg aattagcttg
     3601 gatgagggct ggcgtacagg cacgtggcac caagcagcaa gcacggatca accgtttcga
     3661 agatctaaag gagaacttgt accaagtaaa ccaagaagat gatttagagc tgaacctagc
     3721 gacacaacgt ttagggaaaa aagtactgga aatcaaagat ggcagttacc gtataaatga
     3781 ccaaacactg ctagaacatt tagatttatt gattcaatct cgggaaagac taggtattac
     3841 tggtaaaaat ggtgcgggaa aatcgacatt gcttaatatt ctagctggca gaattcccct
     3901 ggatagcggt acaatgtcta tcggtgaaac ggttcatctt gcgtattata cacaagaaaa
     3961 tgaagaaatg gcaccagata aacggatgat tgcttactta caagaagctg ctgaagaagc
     4021 aaaaacagca gatggcagtc aaatcggagt agcagaatta cttgaacgat ttttatttcc
     4081 aagatttatg catgggacac tgataggcaa actttcaggc ggagaaaaac gccggttatt
     4141 tttgttgaaa ctgctgattc agcaacctaa tgttttatta ttggatgaac cgaccaatga
     4201 tttggacata gcaacattga cgattttgga agattatttt agaagttttc caggtgctgt
     4261 gatcactgtt tcccatgacc gttatttctt ggacaaggta gcagataagt tattagtatt
     4321 ccaaggaaac ggaaaacaag aactttatta cggaaacatg tccagttatt tactgaagca
     4381 aaaagagacc cagcagcctg ctgaaaaagc gaaaccaaaa atacagagca aggaacctgc
     4441 tgaaaagaag aaattatctt acatggaaca aaaagaatgg gaaacgattg aagatgagat
     4501 tgcagaactt gaagaaaaaa tctcattgct ccaagaagag atgaatcatc aaggagataa
     4561 tttcactcga ttacaagaat tgcaaaacga cgtatctgaa acagaagcac aacttgaaga
     4621 aaaaatggca cggtgggaat acttgagtga atgggtagaa gattaggagg aaaaaggatg
     4681 gaacaagcgt atcttgactt aggaagaaaa ttgttggaag aagggcatcg taaagaagac
     4741 agaacaggca caggcacatt aagcttattc ggataccaaa tgcggtttga tctgcaaaaa
     4801 gggtttcctt tgttaacaac gaaacgagta ccttttggat tgatcaaaag cgaattgctt
     4861 tggtttttaa aaggagatac gaatatccgc tatctattac aaaataaaaa ccatatttgg
     4921 gatgaatggg catttgaacg ctacatcaaa agtccagact ataatggacc agatatgact
     4981 gattttggcc atagatgttt gacagatcct gaattcaatg aagaatataa aaagcagagc
     5041 aagctttttt gcgagaagat cctaaccgat gattcctttg ctgaaacata cggcgatctt
     5101 ggacacatct acggctatca atggcgtcac tgggaaacaa aagacggtgg ctttatcgat
     5161 caaatcaaac aagtgatcga agcaatcaaa aagactcctg attctagaag attgatcgtt
     5221 tctgcatgga atccggaaga tgttcctagc atggctttgc ctccttgtca tacgatgttt
     5281 caattttatg ttcaagaggg acggctaagc tgccaattgt atcaacgcag tggggatgtg
     5341 tttctaggcg ttccgttcaa cattgcaagt tatgcgttgc tgactcacct aattgcacat
     5401 gaaacaggtt tagcggttgg cgaatttgtt cacacattag gagatgctca tctttatatc
     5461 aatcatatcg aacaaatgaa agaacaattg agccgagaaa tacgcgcatt tccgacttta
     5521 gttttgaatc aggaaaagca gtcagtattt gattttgaga tggaagatat ccaattagaa
     5581 ggatatgacc cacatccggc gatcaaagct cctatcgctg tttagaatca gaaaggatga
     5641 gaaaatgtta gctgcaattt gggcacagga tgaaaacgga ttgattggaa aagaagaccg
     5701 tcttccttgg cgtttgccaa atgatctgaa attcttcaaa caaatgacag aagcaaatac
     5761 actagtgatg ggacgaaaaa cattcgaagg gatgggtagc cgtccgttgc cgaatcgtca
     5821 gacgattgtt ttgacgagag attcttccta taaagcagaa ggcgtgcatg tcatgcatag
     5881 tgtggaagac gtgcttgctc tggaaaaaga aacagacggt atctttttca tcgctggagg
     5941 gtcagctgtt tatcaagaat ttctgccatt ttgtacgata ttataccgta cagtcatcca
     6001 tcatgtattc gatggagatg cttattttcc acctgttgac tggtctgatt ggtcactgat
     6061 caatactagt caaggagaaa tggatgaaaa aaatcgttat ccgcatcaat ttgaaacgta
     6121 tcagcgtaat gagaatcctg taaaaagcgg gacaggcgaa ttgacgatat aaaaaagcta
     6181 tggatagact agctgttatt tagagctgtc ccatccatag ctttattttt tttattaagt
     6241 aaagaataaa acagagaagt acataaaacc tgcacctaac ataacaaaca aatgccaaac
     6301 aacatgcata tagcggatag aattcaagct ataaaagaat gcgcctatcg tgtaagaaag
     6361 cccgcctgct accatcaaaa taacaccagt cattccaaga gagtgataaa gaggaacagc
     6421 agccacgaca cagagccacc ccataaaaat atagatgatc gtagatactt ttgatactct
     6481 ctcccgttta tgcaacgtca acgctttata tataatgcca agaatcgcaa acaaccagat
     6541 taatacaaat aaagtccaac ctagccatcc tcggatactc agcaaacaaa aaggagtata
     6601 acttcctgca atcaaaagaa aaatcgaact atgatcgaat acttggaaca ccttttttgc
     6661 ttttgtaaaa atcaatgaat gaaacaaagt agacgttaaa aataaaagaa tcatcatcga
     6721 tccatagata gcataagaga caatatggag ggaggaatgc aagtgggcac cttttactaa
     6781 aagaaccact agtcccgcga tactcaatcc agtcccaatt ccatgggtga ctgcattaaa
     6841 cacttcattt aatatttcat acgttcgatt aaatttcgtt ttttccatga aaacggctcc
     6901 tttactaggt gtaattaatg aaaaatcatt atttgtctgt tatactaaaa atagaaattg
     6961 tcttacatgt agtttaacat gaataaggca tcttgttaaa gaaagagaga gagaaaataa
     7021 tgacaaatgt aaaaatcgta acagattctt catgtacgat ggaaaaaaat gtacgcgatg
     7081 ctttaggcat tcgtgtcatg ccgctatcca tcatgatcga cggaacagta tatcccgatg
     7141 atgaccaact agacggcgaa agatttatgg aaatgatggc gaaagcaaaa aatctgccca
     7201 aaacaagcca accaccaatt ggagaattca tctcgatgta tgatgagcta ggagaagacg
     7261 gcagtgaagt gatttcgatc catatgacaa aaggcttgag tggaacagta gaagcagcaa
     7321 gacaagccag taatctaaca aaaacgaaag taacggttat cgacagtgat accactgacc
     7381 aaggattatc ttttcaggta attcgagcag ctgaattagc aaaagaaggt aaaacagtag
     7441 agaaaatcct tgaagaagtc aaaaaagtac aagacaatac aaaattatac atcggaatat
     7501 ccacactaga taacttggta aaaggtggaa gaatcagccg aacaactggt ttgctgtcta
     7561 gtgttttgaa catacgcgtg gtgatgaatt tcgcccattc tgaattaatc cctgtaacta
     7621 aggggcgagg aaagaaaacg ttcgataaat ggtttaataa tttgaaaaat gaactgaaag
     7681 aattgccaaa tgtgcgacaa atcggtattt cacatgcgga tgcaaaagaa ttggcgcaag
     7741 aattcaaaga gggtttgcaa gaattattcc cggacatgca tatccctgta ctgcatacga
     7801 atccggttat cgctacgcat actggtaaag acgcgtttgc aatcatgtat tatgtagatt
     7861 agtcgaaaag aggataagcc ctcagcctga aaggcttgca gcttattctc tttttcttta
     7921 taaaattagg aaaataattg ttttcttttt tcataaagac tacctttttg ataaaatttt
     7981 tgataacatt agaaccaatc gaatacaaga ggtgaaaaaa tgaaattaac gaaacgacat
     8041 ttgatcctcg ttttgttccc attattaatt gcgatcatca tctatgcagg tttagctgct
     8101 tctataccga aagctggccc actaatgaaa ccagtaacta ctgtgagaga agatccaaat
     8161 aaaaaagaaa aacttcacta catagctatc ggagattcgc tgactgaagg agtaggagat
     8221 caaaccaagc aaggaggatt cgttcccctt gtcgcaaatg atctaaaaga ccgttataat
     8281 ttgaccgcgg ttgaaacaga aaactatggt gtaaatggtg aacgaagtga ccagatctta
     8341 aagcgagtaa aaacaaaaga agaaattcga aacaatatca aaacagctga ttttatcaca
     8401 ctgactgttg gcggtaatga tttgatgaaa gttatccaaa ataatttatt cgggttaacg
     8461 gtcaattcct ttaaaaagcc actgaaaaac tatcaggaaa atgtcacaga actattggaa
     8521 gaatttcgat ccctcaataa aaccgcacct atttacattt taggcatcta caatccattt
     8581 tatttgaact ttcctgagat cactgacatg cagacaattg ttaacaattg gaacaaagga
     8641 acagaaacgg tgacaaaaaa aatgccgaac tgctacttta tcccaatcaa tgatttgctt
     8701 tatcaaggat tgaatcagga agtaggtgtc acagaagaag gaacagatca aacaactagc
     8761 acgacagaaa atctaaatgg catcaaaaat aatgtgctct atgaagaaga tcatttccat
     8821 ccaaataatt taggttatca attgatggca aatgcagtga gagatcagct gatcgagaca
     8881 caaaaccaat ggttagagaa ggaggatgat caataaacat gaaaacaaga tctgacgtat
     8941 cttcaaacaa aacaaagaaa acaaattact ggaaattcgc cttccttatc cttcttgggg
     9001 tagtacttgg ttcgaccgta tttttaggca gccgtatttt tgctaatcgg gaaccagaac
     9061 tcccagaaat accagctctt acggaaagac aaggagatcc tgtcttaacg atcaattcaa
     9121 ataaagaaaa agtcaatcaa atcatcagct ttttccttag tgagtatcaa aaagatagtg
     9181 atattgaata taaattttat ttggaaaatg aagcactact caatggtact ttcgaagtac
     9241 taggttttcc tatcgatttc tatttatatt ttgatccgta tgtgatggaa aacggaaatg
     9301 tccaactaaa agctagaagt ctttctatcg gaacattgag tttgccaatc aaagatgtga
     9361 tgaatatgat caagagaaac tataaattac cagattggat cgaagtcaat acagatgatc
     9421 tgactatcat gcttaggttg gatcaattcc gaatgcaaaa cgggatgtat attaaagcag
     9481 ataaaatcga cttagtaaat gatgacattc ggtttagtct atatttgcca aaagatacct
     9541 ctgatgcttc aaacaactca aataccaaca aaactagcac cagcagtact gaaagcaaga
     9601 caaaagaaaa taaaaaatca tcggatgagt agtattagag ggggaatcat aatggaacga
     9661 gcaatatttg caggaggctg cttctggtgt atgatccagc ctttcgacac gcttcctggt
     9721 atccatacag tgatgtcagg atatactgga gggcatgtac cagatccgac ttatgaacaa
     9781 gtaaaaagcc aaacgactgg acatacagaa gcagtggaga ttatctatga tccaacattg
     9841 atttcatatg aacaattagt agaactttac tggcagcaaa ctgatccaac tgacgctttc
     9901 ggacaattcg aggatcgcgg tgacaattat cgcccggtga tcttttattt gacagaagaa
     9961 cagcggatca ttgctgaaaa aagtaagaaa cagttagctg aaagtggacg tttcgaacag
    10021 ccaatcgtta caaaaatcga accagcccaa ccattttatc tagcagaaga agaccatcaa
    10081 gagtattata aaaagcatcc tgaaaatttt aaaagaaacc atgcacgccg ggctgccttt
    10141 atcgaagaaa attggaggaa cgaagggtga atcgaagctt ttttcattat ttaatgacac
    10201 tgcgaggcgg tggcacacat gatgctttat ctgttttcgc tacagaagcc ggtaaagaca
    10261 tccaatttcc taagcacagc tctagctacg aagaaatttc taattatctg gaactgaatg
    10321 tcgactattt gccgtccatg gatatctttg acgaagcatg ggaaaaatat ttagaaaata
    10381 atcatgacat ataagagata gttttgtact ttatgatcaa aaaagttttt tgtccaatag
    10441 cctaattgaa agaattattt ttatttcagc tatctagtcg catttttttg atatttttct
    10501 tataataaag aaagaatgaa aaaaagaggg atggattgat agagattgac aaaaaaacaa
    10561 cgctatatta atcgcttctg gcttgtattc aaatacttac ttgtttctgt attccttgcg
    10621 ttcatgttgc ggggattttt attcatcccg gtaccagtgg aaggcaattc aatggaaaac
    10681 gtgttgaagc aaggtgatat ggttgtgatg gaaaaatttt cggagatcag acgatttgat
    10741 attgttgttt ttcaattagc agatggcacg atttatatca aacgagtaat cggtcttcca
    10801 ggagaaaatg tcagctacca aaatgatcaa ttgaagatca atgggaaagt agttaaggaa
    10861 ccttatctaa ctaaaaacct gaaatcagat catgcaaatg catcctatac gacggacttt
    10921 acactacaag agctaacggg acaatccaag cttccggaag ataattattt tgtacttgga
    10981 gataatcgaa gagtttcaaa agatagtcgt tcgtttggca cgatcaataa aactgatatt
    11041 ctaggtaaag caagatttgt ctattatcca cttgatgaaa taaaatggat ccagtgagaa
    11101 acaataaagc gtgcagtctg ttgagactgc acgctttatt gtttaatcca aaatcagttt
    11161 actaccttag tacgtaaaat caaacagctc gtttaaacac ttttctagcc tctaaagttt
    11221 tgataaatga ataaacaacc attatttgaa tagggatttg gatcatattc ctgatgaatc
    11281 tacctagaaa aattactttg aaatctgcac caaacaaaac agacatcagc caagaattga
    11341 taaaaaggct gactgggatc ataatcaata attgggcagc gatgacggaa ggcaggttta
    11401 tctttttatg atataaaaat aagccattaa taaatccacc aaggaaagcc gtgataagaa
    11461 aagcagcttg aaacgttcct gaaccaaata tcgtgaaatt taaagtgttc gacaacattt
    11521 caagcaaacc agcccagatc ggtccatata gataacccat aagcgccata cttaaaaatc
    11581 cgaatgtgat ttgaaaataa gcaggaccaa tcgctatctt gtataagatg acatcaaggg
    11641 ctaataaaag agcagaggtc gacaacaatc ttgcattcat ttcgggcaat ctaaaaacgt
    11701 cttttgcaat caaagtgttc aaaatattct ctcctttgtc agttaataat aaaaggatac
    11761 cataaaatca ccaatgtgtc atgacacatt ggtgatttac atacactggc atctaacaat
    11821 taaaaaaagg gtgagaagac tgcttttata aactgtaaaa aagagattgt acgaataaaa
    11881 aaaatagagg cggagaatac ttatcgtatc atccgccttt gtttttttgt tataaaaaac
    11941 gtcagattag ttatctgctt acatcttcat ccatacgggt tctaatgtat ttccgtctag
    12001 atcttggact tctaatgaaa acatttcttc ttcaggcatt cccatatcta cttcgtggaa
    12061 agtaccacca ttttcttttg caatttgagc aaattttttt acttcatccg cgttttcaac
    12121 actaaacgaa atcaaagcac cacttgtttt tttattatca gctatttctt tatcttttat
    12181 aaatttttta taaaaatcat gagagagaag cattatccaa aaagtgtcat cccataccat
    12241 tgaaccagct tcttcagtag aaaattcttc atttttttta aaacccagct tttcataaaa
    12301 agcaacggaa cgattgacat cagcaacagg aaaattaaca aaaaccatac tattcatttg
    12361 tttcgtctcc tttctgtttt gttcgtctat catctcttat ttaggaaacg ctgtcaaaaa
    12421 tttagattca tatatagaaa ccaaaataaa aatttgcatc atcaaaaaag tgaagatgtg
    12481 atagaatgag gactggttca ctttcactta actaatagat ggaatttctt tataataaaa
    12541 aagtgaattt atattgaaaa agaaaaaaac aagtaaagcc ggggaatatt ccggtatgaa
    12601 aggaatggag agaacaatga ccatccaatg gtttccggga catatggcaa aagcccgacg
    12661 agaagtttct gaaaaaatga aatatgtgga catcgtattt gaattggtag acgcacgatt
    12721 acctttatct tctcgtaatc caatgctaga tcagatcatt cagcaaaaac ctcgccttgt
    12781 tttattgaat aaagcggatc tggcagatcc acaacagaca aagctgtggc aagaatattt
    12841 tcgtaaacaa ggccatcttg cattagctat cacggcacaa gaaagtaaag gaattaaagg
    12901 tctagtacct gcggctaaag aagcattgaa agaaaaacgg gcaagagatg ctgctcgggg
    12961 gatcaagcct cgcgctattc gggcaatggt cttaggtatt ccaaatgttg ggaaatctac
    13021 gttgatgaat cgactagtag gcaagaaaat tgcacagaca ggaaataagc ctggcgtaac
    13081 aaaaggtcag cagtggcttc gtttaggtaa tgagttggaa ttgttagaca caccagggat
    13141 tttatggccc aaatttgaag atgaagaaat cggcaaaaaa ctagcgctga caggcgcgat
    13201 caaagaccag cttgtccatt tagatgattt agcgatttac ggattagact tttttgcccg
    13261 atattattca ggaagaatca gcgatcgtta tggtttatca atcgagcaag aagaaaaatt
    13321 ggcaccggaa ttactgatgg atattacagc aaaacgtggg tataaagaag actatgatcg
    13381 tggcagcgaa ttagtcgtgc atgatattcg tcaaggaaaa cttggacgat acacactgga
    13441 tcgttgcgaa gaaatggagc aaaaaagtga tgactgaatc catccaaagt atcaaagaac
    13501 gattgaaaag catacagaga atagatgatc ctacactaga atcattaaga aatgatccac
    13561 gcaaaggcgt gcagcaagca ctggcttctt ttgaacgacg attgaaaaag gaagcattaa
    13621 ttaaggaaaa atatgaagaa atgttttgct ttgaaaagca agggtacgca caaggtcacc
    13681 aatggattgc cggcattgat gaagtaggaa gaggaccatt agctggacct gtcgtggcag
    13741 ctgcagttat cttgccgaaa aatgagatca tattagggct gaatgactcc aaacaattat
    13801 cagagaaaaa gcgagaactt ctttatcaac aagttcaaga aaaagccctg gcaatcggga
    13861 tcgggatcgt tgatcagcag acaatcgatg atgtgaatat ttatgaagca agtaaattag
    13921 cgatgatcca agcagtagaa aaattgtcca ttgtaccaag ctatttgttg atcgatgcga
    13981 tgaccttgga tttgcctatt cagcaggaaa aaataatcaa aggggatgca cgatctgtgt
    14041 cgattgctgc tgcaagtatc attgctaaag tatatcgaga ccaattgatg aaagaatatg
    14101 acgtgcttta tcctggatat ggtttctcgc ataatgcagg ttatggaaca aaggaacact
    14161 tagaaggact tgaacgacaa ggaattacgc caatccatcg taaaagtttt gcacctgtaa
    14221 aaaaatattt acaataaaaa acagccgctt ttacgtatct tattagaaag agagacgaaa
    14281 ggcggtttta tgatgtatca aatagaagaa aatttattga aacttgctta tacaaaagga
    14341 atcagtagtc aaggaaaatg ggcacttgct aactggatga tgcagtatcc ttataaagaa
    14401 atcggatttg aagacatcat taagataggt aaaataacca aacatcggga gctgtttcgc
    14461 aaaagttggc aggaaattca cgctaactgg ggcaaaatcc aatcgaaaca gtcgtttatc
    14521 acttgctttg atcccaaata tccccctcag ctgcttcact tgacctatcc gccaattgta
    14581 ctgttttata atggtgatct atctttactt tcttgtaaca tgttaagcgt tgttggtgga
    14641 agacacactt ctgctttagc caaaaaaaca gtttattatt tactaaaacc agtgatagaa
    14701 gctggatatg tgatcgtcag tggctgcgcg aaagggatag atacatacgg tcaccaagca
    14761 gcaatctctc atggtggacg gacaatcgca gtcattggaa cagggataga gcaagtctat
    14821 cctgctgaaa atcgtgactt gcaagaggtg attgcaaatg atcatcttct tttgagtgaa
    14881 tatcctcctc aatccggccc gaaaaagcat tgttttccaa tgagaaatcg gattatcgca
    14941 gctttgtcta aaggaacatg tgtctttgaa gccaagaaaa aaagcggctc tctgataaca
    15001 gcacagcaag cattggatct tggaagaaca gttttttctg ttccaggaaa tattttaaca
    15061 gaacattcta ccggatgtca tcatttgatc caagacggtg cagtttgcac ggtttcgggt
    15121 gaagatatct tggcagaatt taaagaataa caccatactt ttcatttgct tttcttgaca
    15181 aacgttttct gaatggatat gattagctac gatttgtatg aatcctcttt taatagtagg
    15241 aacacgacaa tggaaaggag tccattcagt aatggcgtat aaatatttag tgattgtaga
    15301 atcacctgca aaagccaaga caattgagaa atatcttgga cgaaactata aagtaatggc
    15361 cagtgttggc catataagag atttaccgaa aagtaaaatg ggtatcgatt ttgaaaacaa
    15421 ttatgaaccc cattatattt ctatacgcgg aaaaggcgat gtcatcaaaa gcctgaaagc
    15481 cgcagcaaaa aaagctcaaa aagtttactt ggcaagtgac ccggatagag aaggagaagc
    15541 gattgcttgg catttagcgt acctacttgg gttggatctg aaagaaaaaa atcgggtggt
    15601 cttcaatgaa atcacaaaag acgcagtcaa agcagctttt aaggaaccaa gaacgatcga
    15661 tgtagattta gtagatgcac agcaagctcg tcgtacctta gacagaatcg ttggttattc
    15721 gatcagtcct attctctggc gtaaggtcaa gaaagggtta agtgcaggac gtgtccaatc
    15781 tgtcgcttta aaaattatta ttgaccgtga aaaagagatc cgagaatttg ttccagaaga
    15841 atattggagc atcgacggta attttaaaaa agctcgcaag aaattcaaag caaatttctg
    15901 gggaatcgac ggtaagaaaa agaaattacc agatgcacaa agtgtaaaag aagtcactgc
    15961 tagaattgat ggaacagact tcgaagttaa aaaagtagaa aagaaagagc gcaaaagaaa
    16021 ccctgcgaat cctttcacta caagtagcct gcaacaagaa gctgcccgta agttaaactt
    16081 tcgaacaaga aaaaccatga tggtagcaca gcaattatat gaaggaatca ctttgggtaa
    16141 acaaggaaca gtcggcttga ttacatacat gcgtactgat tctacccgga ttgccgatac
    16201 agcaaaagca gaggccgcag catttattga agatacgtat ggaaaagaat tttctagaca
    16261 tgatcaccga aagatcaaaa atgctcaagg cgcacaagat gcccatgaag ctgtgcgtcc
    16321 aacgagtgtg cttcgtacac cagatgaact caaacaatat ttagataaag atcaattgaa
    16381 actttataca ttgatctggt cacgttttgt ggctagtcag atgacaccag ctattttaga
    16441 tacaatgaaa gtgacattag aacaaaatgg tgtcatcttc attgcgaacg gttcaaaaat
    16501 caaattcaaa ggattcatgc aagtgtatgt ggaaggtaga gatgacggca aagaagagaa
    16561 agaaaatatt cttccagaac ttgaagaagg cgatgtagtc caatctgttg atattgaacc
    16621 aaaacaacat tttactcaac caccagcacg ttttagtgag gctacgttga ttcgtgcact
    16681 agaagaaaac ggggtagggc gaccttccac ttatgcacca acacttgata cgatccaaag
    16741 acgctactat gtaaaattga cacaaaaaag atttgagccg actgaactag gagaaattgt
    16801 caattcatta atctgtgaat tcttccctca aatcgtcgat atccatttca cagcagaaat
    16861 ggaaggagat ttggataaaa ttgaagaagg cacggaagcg tgggtcaaag tggttgatcg
    16921 tttctataaa ccgtttgaaa aagaactgac gaatgcggaa gaaaaaatcg aaaaaattca
    16981 aatcaaagat gaaccagcag gattcgattg tgatgtctgt ggacatccga tggtgataaa
    17041 attagggaaa tatgggaagt tttatgcttg cagtaatttt cctgattgtc ggaatacgaa
    17101 accaatcgta aaagaaatcg gtgtgacttg cccagtatgt catgaaggac aagtcattga
    17161 acgaaaatct aagaaaaaca gactattcta tggttgttcc cgtttcccag aatgtgaatt
    17221 cacttcttgg gataaaccag tgggcagaaa ttgtccaaaa tgtgacggat atctggtcga
    17281 gaagaaagtc aaaggcggta aacaagtagt ctgtgtaaat ggagattacg aagaagacat
    17341 ccaaaaataa tagaaaagag gttctttcat tgaaaatggt aaatgttgtt ggagcagggc
    17401 ttgccggaag tgaagctgct tggcagatcg cacaagcagg agtccctgtc cgtttatatg
    17461 aaatgcgtcc agtcaaatcg actgaggcgc atcacacaag taattttgct gaactagtat
    17521 gttccaattc attaagagga aacagtttgg ctaatgcagt agggttattg aaagaagaaa
    17581 tgcgccgatt gaattcggta gtaatcactt cggcagatga aacagcagtg ccagctggtg
    17641 gtgccttagc tgttgaccgc gattcttttt ctgaaacaat tacccgaaaa gtcaaagagc
    17701 atccattagt aacagtgata aacgaagaaa ttactgaaat accggaagga atcaccgtag
    17761 tagcaacagg acctttgact tctcatccat tagcagaaag catcaaagca ttcaatggtt
    17821 cagatggttt ttatttctat gatgcagctg caccaatcgt agacaaagct acgatcgata
    17881 tggataaagt atatttgaag tcccgctatg acaaaggaga agcagcttac ttgaactgtc
    17941 cgatgacaga agaggaattc aaagctttcc atgaggcact aatcaatgcc gaagtggcag
    18001 aacttaaatc ttttgagaaa gaaaaatatt ttgaaggatg tatgccaatt gaagtaatgg
    18061 cacagcgagg attcaaaacg atgcttttcg gacctatgaa accagttgga ttggaagatc
    18121 ctaaaactgg caaacgtcct tatgctgtta tccagctgcg tcaagacaat gctgcagctt
    18181 ctttatacaa catcgtgggc tttcagactc acttgaaatg gggcgagcaa aaacgtgttt
    18241 tccggatgat tcctgggtta gaaaatgctg aattcgttcg ctatggtgtg atgcaccgaa
    18301 acagctttat gaattcgcca gaattgctga aaccgactta ccaatcaacg aaacgcgaag
    18361 atctgttctt tgcaggtcag atgactggtg tagaaggcta tgttgaaagt gcagcgagcg
    18421 gactacttgc tggaatcaac gccgcacgct tagcaaaaga aatggaacca gtcgagtttc
    18481 cacaagaaac agctattgga agtatggctt actacattac tcatgcggaa ggaaaacatt
    18541 tccaaccgat gaatgccaac tatggtctgt ttcctgaact gccagagcgc attcgagaca
    18601 aaaaagcaag atatgaagca attgcacaac gagctttaaa agctaatgag gcaataaaaa
    18661 cagaattaga aaaaactgca atcccaaatg agtaaggaaa ctccatgaag agggtgagac
    18721 ggatagtctc gctcttttt
//
LOCUS       DACDXO010000054        17951 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E23605_2017
            SAMN12639989-rid9881463.denovo.055, whole genome shotgun sequence.
ACCESSION   DACDXO010000054 DACDXO010000000
VERSION     DACDXO010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639989
            Sequence Read Archive: SRR10023389
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741914.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:42:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,972
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 33 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 17 of 73
            Pseudo Genes (multiple problems)  :: 10 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17951
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639989-rid9881463.denovo.055"
                     /isolate="E23605_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(26..1522)
                     /gene="lysS"
                     /locus_tag="IWM35_002054"
     CDS             complement(26..1522)
                     /gene="lysS"
                     /locus_tag="IWM35_002054"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HAP9919898.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKAGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(1633..2637)
                     /gene="dusB"
                     /locus_tag="IWM35_002055"
     CDS             complement(1633..2637)
                     /gene="dusB"
                     /locus_tag="IWM35_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HAP9919899.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVACREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(2638..3525)
                     /gene="hslO"
                     /locus_tag="IWM35_002056"
     CDS             complement(2638..3525)
                     /gene="hslO"
                     /locus_tag="IWM35_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HAP9919900.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(3742..5853)
                     /gene="hflB"
                     /locus_tag="IWM35_002057"
     CDS             complement(3742..5853)
                     /gene="hflB"
                     /locus_tag="IWM35_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HAP9919901.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(5943..6488)
                     /gene="hpt"
                     /locus_tag="IWM35_002058"
     CDS             complement(5943..6488)
                     /gene="hpt"
                     /locus_tag="IWM35_002058"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HAP9919902.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(6488..7933)
                     /gene="tilS"
                     /locus_tag="IWM35_002059"
     CDS             complement(6488..7933)
                     /gene="tilS"
                     /locus_tag="IWM35_002059"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HAP9919903.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8053..8523)
                     /locus_tag="IWM35_002060"
     CDS             complement(8053..8523)
                     /locus_tag="IWM35_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein S1"
                     /protein_id="HAP9919904.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(8569..8994)
                     /locus_tag="IWM35_002061"
     CDS             complement(8569..8994)
                     /locus_tag="IWM35_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HAP9919905.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9061..9330)
                     /locus_tag="IWM35_002062"
     CDS             complement(9061..9330)
                     /locus_tag="IWM35_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HAP9919906.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(9341..10936)
                     /locus_tag="IWM35_002063"
     CDS             complement(9341..10936)
                     /locus_tag="IWM35_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAP9919907.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(10951..14472)
                     /gene="mfd"
                     /locus_tag="IWM35_002064"
     CDS             complement(10951..14472)
                     /gene="mfd"
                     /locus_tag="IWM35_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HAP9919908.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(14489..15049)
                     /locus_tag="IWM35_002065"
     CDS             complement(14489..15049)
                     /locus_tag="IWM35_002065"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HAP9919909.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            15402..16376
                     /locus_tag="IWM35_002066"
     CDS             15402..16376
                     /locus_tag="IWM35_002066"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HAP9919910.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            16546..17946
                     /locus_tag="IWM35_002067"
     CDS             16546..17946
                     /locus_tag="IWM35_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HAP9919911.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
BASE COUNT         4875 a         3974 c         3238 g         5864 t
ORIGIN      
        1 aattccttag acagctttta ttcgattatc tcattgttgg gaatagcaaa acatcccgaa
       61 ttgattgtgc atcagttagc aacattacta atctgtcgat cccgatgcct agaccaccag
      121 ttggcggcat accgtattct aaggcttcca agaagtcttc gtctacaccg tgagcttcgt
      181 catttcctag ttcacgttct ttttcttgcg cttcgaatct ttctctttga tcaattggat
      241 cgttcagttc tgtaaaggca ttagcaaatt cgcgccccac tatgaatagt tcgaatcgat
      301 ctgtgaatcg aggatcttct ggatttttct tcgctaaagg cgaaacttcg actggatgtc
      361 cgtatacaaa cgtcggttgt gtcaatgttt cttcaacaaa tgtttcaaag aattcattga
      421 taacatgtcc gacacccata gcttcggtta cttctacatt gtgtttttcg gctaaagcta
      481 atgcttcttc caatgtcatt tcttgccaga agtctacacc agtttgttct ttgattgcat
      541 ctaccatatg gatgcgttta aagtcacttt ctaagtcgac agcttgtcca tcatatgtga
      601 tttttgctgt tcctagtact ttttcagctg cgttacggat aataccttct gtcagattca
      661 ttacatcatt gaaatcagta tatgctgtgt atgcttctaa catcgtaaat tctggattat
      721 gtgtagtatc aattccttca ttacggaaaa cgcggccgat ttcataaact ttttccattc
      781 cgccaacaat tagtcgtttt aaatgaagtt ctagcgcaat acgcaaatat aaatcaatgt
      841 ccagtgcatt atgatgcgtg ataaatgggc gtgcggcagc tccacctgcc tcattgtgca
      901 atactggtgt ttctacttca atatagccat tgccatctaa atagcgacgg atttcgctga
      961 taatttggct tcgtttcata aatcggtcaa agctttcgcg attactgatt aaatccaagt
     1021 agcgttgtct gtagcgttgt tcaatattcg ttaagccatg gtatttatct ggcagtggac
     1081 gaagtgcttt tgacaaaatc gtgatttctg aagctttgat agaaacttcg cctgtgtctg
     1141 ttttcatgat ttgtccagtt acaccaaaga aatctccaag gtctgcatgt ttgaatactt
     1201 cataggcctc atcacctact tggtcttttc ttacataaat ttgaatttgg ccttcacgat
     1261 cttgtagatg cgcaaatcca gctttccctt ttcctcggcg agtcatcata cgaccagcta
     1321 cactagcggt caatcccatt tctgctagtt cttcttttgt tcttggatca aatagttcat
     1381 gcaattcttc tgagttgtgc gtgcgttcaa atcgttttcc aaatggatcg attccatctt
     1441 cacgcaattg ttccattttt tgacgacgta caagcatttg atcatttaaa tcttctactt
     1501 gatgttgttg ttcctgtgtc acaaaagttc ctccattctc atgttaatct ctttcctata
     1561 atgccaatga aacacaaaaa aagcaagccg aacttggaat ttcggcttga aaattcacga
     1621 aaaacttaga aatcacaacg tttgtttcaa tagtactttt tccgcttttt ctacaaattg
     1681 atccagcagg tcattgattt cttgctgttt ttctgtctga ttgattgcta ctttcacttt
     1741 cgctgctcga ggaatccctt ttaaataata agccgcatgt gttctgaatt ctctacaagc
     1801 tactttttct cctttgagat ctgctaagcg ttgtaaatga agtttagctg tagcaatttt
     1861 ttcggctggt gttggttcag gaatcagttc ccctgtttcg agataatgct gtgtgcggtg
     1921 aatcatccat ggattgccca gcgctgcacg accaatcatc acaccgtctg caccgacata
     1981 gtcaagcatc cattttgcat cttctggcgt tttgacatcc ccgttcccca taaatggaat
     2041 cgtcaaatgt tttttcactt ctttcaatac ttcccagtcc gcttttcctt catacatctg
     2101 aacccgtgta cgtccatgca tagcgatggc acttgctcca gctgattgtg ctgctaatgc
     2161 attttctaca gcaaagacgt gctcttcatc ccaaccaata cgcatcttga ttgttactgg
     2221 gatatttact gctgaagaaa cagcttggac catttcgtat actttctcag gatctaaaag
     2281 ccattttgct cctgcttctg ctttgattac tttatttact ggacagccca tattgatatc
     2341 tattatatcg gcagctgtgt tttcttcgac aaattgagca gcttcaacta gactatcttt
     2401 atttccgcca aatatctgta aacttaatgg atgttctgtt tcttcaatgt ggagcatctc
     2461 taaagttttt ttgtttctga aatgaatacc ttggtcactg atcatttcac atacgactaa
     2521 gcccgcacca aattctttga cagtgactcg aaatgctgaa tttgttattc cagccatcgg
     2581 tgcaacaacg acacgattag ggatttccac attgccaatt ttccatgtgt gttccactta
     2641 aatccctcct aaggcttctt tcttcaatcc atctaattct tcttcgctat acttgtattt
     2701 gttattacag aaagaacaaa ccgcttctgc accgtgatct tcgtctatca ttgcttggat
     2761 ttcttctgca cctaacgcga taattgcttc tgcaaatttt tcttttgaac agtcacattt
     2821 gaattgaaca ggcatttttt ccaaaatgtt caattcttct gtaccaagca atcctttcaa
     2881 tatttcttct ggtgtctgtc cttcatctaa gagggtagag attcttggtg tcgtttttaa
     2941 ttgttcttcg atcttgctga ttgtttcttc atcggcacct ggcatgattt ggatcatgaa
     3001 tcctcccgcc gtcttgatgc tgtcgtcagt attgaccaat acactcagtc caacagctga
     3061 agggatttgt tctgagatag caagatagta agtaaaatct tcagcgattt ctccagaaac
     3121 aatcggcgtt tgcccagaaa aaggttcttt taatcctaag tcttttgtga cagtaaacat
     3181 tccttgtgtt ccaactgctc cccgcacatc gattttacct tcactattta gaggaagact
     3241 gagatgcgga tttttgatgt aacctttcac atctcccttt ccatttgcat ctactacaat
     3301 tcctgttgct ggaccatcgc cttggattcg aacagttagt ttgtcttctc ctttgagcgt
     3361 cgcaccaagt agcagactac cgaccaatgt ccgtcccaaa gcagctgagg aggtgtgcca
     3421 agtatcatga cgtctttgtg cctccgctac ggtatttgtt gcttgaacgg cgtatgctcg
     3481 gacgaatccg tcgtgagcaa gtgccttaac caaataatct ttcatttgtt atgtactcct
     3541 ttttccgttt ttaaactcat agtgaatcat actagcaact actgcttttt tcaactttgg
     3601 aatgcttctt ttaaaaataa ttcagaatca tctagaaaac aggacatcac cctatttaca
     3661 acttaaataa agaaatctcc cgaaaaccgg tttttgttcc ggttttcggg agtcatatca
     3721 gatcaattga cctatatttt tttatttgta ttgatcgtct gaatgattat cttctgattg
     3781 ttgagaatct gaatcatgtt ctgctttatt ttcctcattc agttcatgtt ttgcttcttc
     3841 aaaatcacgt ttttcttccg cttgtttttc agcgtctttt tcttccaaag cacgttttgc
     3901 ttcttcaaaa gttttcgctt ctttttcgct tggataatca tttgactcag taccttcagg
     3961 catcacaccg tgttcaaaca atgacttgat tgctttggca tccaatgttt cgaattccaa
     4021 aagtttttca gcaatcagtt tatgctgtgc acgatgagct tcgatgattt ctcgagcttt
     4081 atcatgagct tccatcaaga tttttcgtac ttcttgatca atctcgaaag cgacttgttc
     4141 cgagtaagct tttgtttgac catagtcgcg gccaacaaac acttgatggt ttccttcata
     4201 ttgaacagga cctaaacgat cactcattcc atattctgtt accatgcttc gtgctagagc
     4261 tgtcgcttgc tcaaagtcat tggaagcacc agttgattgg acattgaaga tgatttcttc
     4321 agctgtacgt ccaccaagta agcctacgat ttgttcaaac atatcttctt tcgtcatcaa
     4381 gaactgatct tcttttggta aagcgatcat atatccgccg gcgcgtccgc gagggatgat
     4441 cgttactttg tggacgaccc gagcgcgact caatactaat ccgacaatcg tatgaccagc
     4501 ttcgtgataa gcaaccattt cgcgttcttt tttgctgatt acgcgatctt ttttagctgg
     4561 acctgcaatc acgcgatctt ccgcttcatc gatatcagaa gcatcgattt tctttttatt
     4621 tcttctagca gctactaacg ctgcttcatt caagacattt tctaaatctg ctccggcaaa
     4681 acctggtgtc tgttgagcca caactttcaa gtcaacgtca tctgccatcg gtttattccg
     4741 tgcatggact cgcaagattg cttcacgacc ttttacatct ggacgtccaa ctaaaatctg
     4801 gcggtcaaaa cggcctggac gtagtaacgc tgggtctagt acatcagaac ggttggttgc
     4861 agcaatgacg attactcctt catttccgtc gaagccatcc atttctacaa gcaattggtt
     4921 caatgtttgt tcgcgttcat catgtccgcc gcccataccg gcaccacgtt gacgacctac
     4981 cgcatcaatt tcatcaatga aaatgatagc tggtgcgttt ttcttcgctg tttcaaacaa
     5041 atcacgaaca cgacttgctc caacaccgac aaacatttct acgaaatcag aacccgagat
     5101 cgagtaaaac ggtactccag cttctccggc aacagctttt gctaataagg ttttacctgt
     5161 tcctggaggt ccttctaaaa gtacacctgc cgggatacgg gcacctaatt caacaaatcg
     5221 gcgaggatct ttcagaaatt caacaacttc aactagttct tgtttttctt cttccgcacc
     5281 cgctacatca gaaaaacgta cgcgattagc ttttttgtct gcttctttgg cttttgattt
     5341 accaaagttc atgacacggc cgccaccgcc accgccgccg ccttgttgtc ccatcatcat
     5401 gtaaaagaaa aagatgatga ttacgattgg caagaagcta agcaataggg aaacccatat
     5461 accgctgctt gattcttctt ttactgttgt agcaactttg ttttcttttg ccagttctgt
     5521 tacttggctc aatgttgtat catttggcaa aatgattgtt gtgaaatggg tagtagtcgt
     5581 atctgttgaa cctaagatag ctaacccacc gctgttagcg acggtctgct gttctttgta
     5641 ttcaccagtg atcttatata caccatttgc aggctgcact tcaaattctt tgatcttgcc
     5701 tgcttgtagt tgttctgtga attttgagta ctcgatatct gacgatcgct ggccgttatt
     5761 accaaaaatg aaataaacga ccattaccat cgctaagatt acgaggacat aatacaaggc
     5821 atttttcatc atgccgttgt tctttttgtt catagttgcc ctccctaagc gtcaatttaa
     5881 tttaacagtt ttttatcaat cctgaccatt ctattgtacc ataatttaat tgagatacag
     5941 cattaatttg attgataaac actaggtttc aatacaccga tgtacggaag gttacgatat
     6001 gcttctgcat aatccaagcc ataaccgacc acaaattcat ttggtacgtt gaagccaaca
     6061 taatctgctt cgatatttac tactcttcct tcaggtttgt caagtagtgt aacaattttg
     6121 actgattttg ctttgcggta tttgaaaaga tccactaaat aagcaagtgt tcttccgctg
     6181 tcaatgatat cttcaacgat caacagatcg cgaccttcta cattcgtatc taagtctttg
     6241 acgattttta cttcccctga cgagactgtt gcattcccgt agcttgaaac atccatgaaa
     6301 tccaattcca aatacgtatc gatagaacga acgatatctg ccatgaatgg taccgctcct
     6361 tttaagacac ctactactag gggattggtg ttttggtaaa tttctgttaa ctctttgcct
     6421 agttctttgc aacgaacttg tatctcttct tgtgagatca aaattctctc aatatccttt
     6481 tccaacatca ggttctcctt ccaattgctt ctttttgata tttgtaaaga agtatgtagt
     6541 gtattttatc agtttcttca cgaatactca aatatgaaaa ggcaaaaggt atcaagctat
     6601 atatatttct tttggcatct gtaatgatcc aagcatgctg cctttgtgaa ttaggtactt
     6661 tctgatcgat aaaatatcta gatacttttt ttgtgagatt ctctgttaat ttaatgcggt
     6721 ctcctgcttt tcttttttcg acatacagtt gcacatcagc aggaatccaa atgtcatgag
     6781 aaaatagaga ccatgaatct gttgctttga tgtcccctgc attttcaggt ttgaccagac
     6841 caatccattc tgtttcattc aaatatattc cttgttctgg aatcagcaac aagcgctggt
     6901 cttctgtctg gctagttgct tgtttttctt ttttagagag tacaacaaaa tgatactctc
     6961 ttttcagtat ccaattattg ccaatactga gttgccattg gctttttttc tgttgccact
     7021 ggtctaaaat cgactccatt tgttgttctt tcaaaataat tcctgttttt gagaatgctt
     7081 tgtcgaaaaa ggagtaaaaa aaatagtatc gttcagcatc tgtcatttct tcaatcattt
     7141 ctgcagaaaa tcgaaattgt tcctcccact gttcgacttc cttttcgatc aactgtccca
     7201 tatatttttg aatgacttgg tctgcccatt ggatctgttg cgaaaattgc tggaaatgtc
     7261 tcaacgcctg tgtattttct tgtttcaatt gaggaactac caaatgacga agccgatttc
     7321 tctggatatc taacaattgg tttgtctgat cctcaaagta aacaagctgc gattccgctg
     7381 cataagtata taggtcttct ttgctaaaac tcagcaacgg acgaatcagt ctgcccgtag
     7441 caaatggttg tacttctttg attccagaaa aagtatttaa ctgtccccca cgaacgattt
     7501 tcattaacac cgtctcgatt tgatcatctc cgtgatgagc agtcatcaac gtgtcatatt
     7561 gtccttcttc catcacgcga gtgaattctt gataacgaaa gttacgagct tctgtttcta
     7621 tggcggattt ggctggatct tcccaaatac ttacatataa agggagattc ttctcttcac
     7681 aatatgagcg aagataggca gcttcttctg ctgattcttc tcttaattgg tgattgatgt
     7741 ggacgatacc taaagaaaaa ccgatttggc gttgcacttg taaaagtaaa tggagcaaaa
     7801 ccatcgaatc aacaccacct gataccgctg ctaaaattct cgatccttgt tgccagaatc
     7861 cttgttcatt cccgtgccga ataaatgact gctgtaatag cggtgctttt ttcccgcctt
     7921 tttcaacgtt catcctttcc acaccctttc ttcctaaaaa aaagagagcg ggaaaacgtc
     7981 taaaacgaca attaacctgc cctctgttga tactatgacc caaatagtcg cactatcatt
     8041 atttgcggct ttttagctgc gacgtccgcc acggccaccg cgctttcctt ctgtgtttcg
     8101 cttcaatgaa gataagcggt catcgctatc tttcaaaaat gaactcatca atgaatcgaa
     8161 gtcttctttt tgagctgaag gttgactgcg agaaaaagat tttttctgtg ctggacgatt
     8221 tcggttgtca cggggatctt tttgttgata ttttttagga cggctttctt ctccagattc
     8281 agcaggttgt tcctgcgctt tccggataga tagtccgatt tttccatcat catttactga
     8341 aataacttta actgttactt catctcctac acttaaaaca tcatggatgt cttttacata
     8401 gccatttgac acttcgctaa tatgaactag acccgttttt ccagcaccta gatcaataaa
     8461 cgcaccaaaa tttgtgattc ctgatacttt tccttgcagt tttgctccta cctcgattga
     8521 cataaaaaaa atgttcctcc taattatttc ccttttttat attcctgctt attttgcagt
     8581 tgtagaacta gttgattgat ctgtttcatt tgtcgagctg tctgtatttg attctaatat
     8641 aggataaact ttttcaccgt cttttgagta aaagaatttg cttctggcaa gtttagctac
     8701 atactcatca tctttcagca atgaaacatc ttggtgcagc tgatcaacgt ctgcgttgac
     8761 ttctttcatt tcagtatttg cttcgactct caattcgttc aattgatgca tcctttgcat
     8821 gtcatggaaa atctgcacac ctattatcgc gaaaatgacc aaagcaccaa caaaaattgc
     8881 agccagtctg cgtcttcgaa aaatcagttg acgttgttgt tgctgaaatt cagcatactt
     8941 tttctttgca tattccgtat ctagcgcagc gattttattg ctttttttcg tcacttggat
     9001 tcgctccaat ctttttgatt gtctttttca ttataccaat atcgtgaaag cttgtctaat
     9061 ctatttctct agtgtttctg tgcgattttc agaaataatt tcgtacattt tttgtgcatc
     9121 ttcttttttg gttgattcat gtaattccgt aattttcact tccaacgttt tgttgccaaa
     9181 ctggactttc aaaatatcgc ctactttgac atcactggaa gatttcgcca atttaccgtt
     9241 gatttgaatt cttcctttat cagctacttc ttttgctact gatcgtcttt tgatgattcg
     9301 ggaaattttt aaaaatttat ctaatcgcat atcttattcc tcacttttta atttttttat
     9361 ttaatcgcag gattttctta ccaaaaggca acattagcca ttctcgtact gtaaacagac
     9421 gaagacgaat cactgcataa atgaacacag aagccccgat cccaactcct atcacgctga
     9481 caaaaaaagc agtggaacga tgagttacag gaccaaatac tatattcaat acaccataat
     9541 acagcagcag tactactgtc atacaaagaa ggctgaacat caacttccgt ccaaaatgcc
     9601 gttctttcca aaaagcattt agctcttttg tttcaattcg aacaaaatac caaagcgtca
     9661 cacctaatcc taaaagggta gacaggcttg cacctgctgt tcctaataaa ccggtcagaa
     9721 aagaagtagt caatcctttt accaatagtc cccatcctgc gccttttaaa gaagggcgaa
     9781 acgagttctt gctttgagca atactttgat aagcttggat gactgcagtg aaagcaatgg
     9841 aaaaaacaaa taatacaagc gctgcatttc ccgcataatc tttaaacaac gcataattga
     9901 tataagggag cagcaaggct aatcccaaag acgcagctaa tgctaaagcg gtcgtcaatc
     9961 taagatagat tttggctgtc tgtaaaaact gacgatagat ccggttggtc aaatgacgag
    10021 tcaacgcagg caggaatgtc gcacttaatg cagtggcaat cactaaacct aattgtacta
    10081 aaggctgacc tcgatcatag accccttttg ctatcttcgc accgtattct gacagtccgt
    10141 aaacttccaa agcatttttt acaaagaaag agtcgatcag ctggaaaaag ataagcaatc
    10201 cactataaat cgaaaccagt ccgccttcta ccagaaaacg ccggatcaag ggctttgacg
    10261 gctgtttgaa caatggaaac cgtcggaagc ttaacgctcc tccatgaatc ttttgttcat
    10321 agtagtgaag gatcccatat gcgcataaac cgccaaaaac agctccactc attgctgccg
    10381 tccctgtttg gtacaccgtc catccaagct ttctaaaagc aaacgcacta cagacaatca
    10441 caaaaacacg aacgaactgc tcaactactt gagaaacagc agttggttcc atcaagaatc
    10501 ttccttggaa attccctcga taaaaagata gaccaggcat cagcaaaaag gtaaaggaaa
    10561 ctacttggat cagcggtttc aattgtatgt ctcccatcat ccaagcaatc cagctgctaa
    10621 atgcaaagac aaacaaccac aaaaagacac ctgtccaaaa tactaaggga taaagatttt
    10681 gcaaagcttg ctttcgctcg aaaggattct ttctttctgc aacgtacttt gatatgaact
    10741 gcggaagacc ggataaagct aaagtcattg ctatcccata aattggataa acttgctgat
    10801 aaacataaaa accttcgtcg ccgactaagt tttggagggg aattcggtaa agggcactta
    10861 atacttttgc tatgaaagaa gcaatcgtca atacaaatgc tccttgcatg acgcgccgca
    10921 tttctttatg atccaaatca ttgctccttc ctacgctgac agatattttt gctcacgcaa
    10981 cgctttgaca aacttttgga tttcctgaag ccatactgct tcttccattt ggttggggat
    11041 cgtcaagcgt atcaccatct gttctttttc aacgcctaga ctggctttta gttttgtcgc
    11101 tgtcagtgct tcgaataact gttccacact atagcgtttt gttccaactt tacttagagt
    11161 aaagactata tgcgcctcct gtttccttat cgtttctagg agcgctcgat cgccatccat
    11221 tttgatctgc ccaattgtca agagatgtgc tacttcttct ggatattctc caaaacggtc
    11281 gagcagatca tcttctagtt catctagcat ttcttgcgaa tcaagttcac gaattcgttt
    11341 atagatctcg attttctgcc gttgatctgc cacgtatgtt tcaggtagat acgcatcgac
    11401 accgaggtcg atctcaacag tggtcttctc cacttgacta ttctttcctt gtttacgaga
    11461 aacagcttcg cttagcattt gagaatacat atcgaaaccg actgcatcaa taaatccatg
    11521 ctgctgagca cctaagagat tacctgcacc gcgaatggaa agatcccgca tcgcaatctt
    11581 gaatccagat cccagttctg taaaatcttt gattgcctga agacgttttt cactgacttc
    11641 gttaaggatc ttctgctgct catacataaa gtaagcatat gcgacacggc tgcttcttcc
    11701 tacccgccct cgcaattggt ataacgtgga taatcccata taatcagcgt tctcaacgaa
    11761 caacgtattt gcgttcggga tatctactcc tgtttcaatg atggttgtag tgacaagaac
    11821 atcatattga ccttcaataa attcaaaaag cgtattttct aattgcgcct ctgtcatttg
    11881 cccatgagca taaccgatcc gtgcttccgg aacgagttct tggatttctt ctactttttg
    11941 ttcgatcgtt tctacccgat tatataaata gaatacttgt ccacctcgtc ccatttctcg
    12001 atggattgct tcgcgaatag ccccaggatt tttttccata acatacgttt ggatcggata
    12061 acgattcgca ggcggtgttt cgattactga aagatcccgc acgcctaaca tcgacatatg
    12121 caaagttctc ggaatcggtg ttgcagtcag agtcaaaaca tccacttgtg cacgtaactg
    12181 tttcaatcgt tccttatgtt ttacaccaaa acgttgttct tcgtcaatga tcagtagacc
    12241 caaatcgcta aattcaatat cttttgacaa aattcggtgg gttcctacaa caatatcaac
    12301 ttgacctgta cgcagttgtt cgatggtttc tttttgttgc tttcttgttc tgaaacgact
    12361 taatagcccg atatttacgg ggaaaccttc aaatctttcc agcatcgttt catagtgctg
    12421 ctgtgccaag atcgttgttg gaactaaaaa ggctacttgt ttgctttcct ttatcgcttt
    12481 aaaggccgct cgaagcgcta cttctgtttt cccataacca acatcaccta cgagtagtcg
    12541 atccattggt ttttcttttt ccatatcccg tttgatctcc gctgcacttc tcaactggtc
    12601 atcagtttca gagtagggga aggcattttc gaattctttt tggtaggcgt catcaggtcc
    12661 aaaagcatat cctttttctg actctcttgc ggcatataat ttgattagat cgtcagcgat
    12721 atcctcgatt tttgaagaga cttttcgctt ggttttcgtc cattcacttc cgcctagctt
    12781 attgatgcga ggcgctttgg cttcagaggc gacatatttt tggatcagat tcagttgagt
    12841 aacaggaata aacagtttgt cgtcgttttg atacaaaata gtcatatagt cttggtgaac
    12901 gccatctact tctagcgttt ccatcccaat gtatttcccg ataccatgat tggcatggac
    12961 aacgtaatct cctgctttta actcattata gcttttcaat cgctctgcgt ttgttactgt
    13021 ttgacgtctc gtttgttttt tcgtcgtttt ttggaaaatt tctttttctg tgatagctac
    13081 tattttttct tgaggaagtt caaaaccagc ctgcaaagct ccttcaacta attgtacctg
    13141 tcctctgatc aatgcatttt tttctgtttc tactactaat atgtcttcat ctcgaagcat
    13201 ttgttctgct ttgcgtattc tgtcctttgt cggaataaag atcacaacag tctgtttttg
    13261 cttgcgccaa cggtccattt ctgttttcag caatggcatt tgtccgaaga actgttgcat
    13321 cgtccgatat tgaaaattat ggattgcttg gaaacgaaga tttcccatcc ctttttggaa
    13381 gagtgagaaa aaggttgtgg tgaacttttc cttctgcacg attttttgta tatctgctga
    13441 aaatgtctgc tcagaaaaca cccgcaattc gctgattttt tgagtctgcc attctgccgc
    13501 ttccctttcg atttcccgat ttgtttccat gattcgctga taatcatcaa cgactagtaa
    13561 actatttgaa gggaaataat ccaagatcgt cacagattct tggtaaagaa gatctgagta
    13621 gaattttgct tcttctgtcg ggacacctgc ttgccagctt gaagtcagct gaccgaagta
    13681 atcttgcaaa aactcgcggt ctgcttcttc tgtggtcgca gcaagccgtt tttctagcat
    13741 tttttgcatt tgctctattc catgttctag atcctctggt ccgtagactt gttcactcgt
    13801 tggggaaacc cagatttctt ccgttttttc gattgagcgt tgtgtttctg cttcaaaata
    13861 ccgcatagaa tctatctcta catcaaatag ttctacacga acaggatagg ttgtcgttaa
    13921 cgggtaaata tcgataatgc ttcctcgaat actgaattct cccggttttg cgaccatctc
    13981 ttctcgtaca tagcccatca aagtcaattg gcgaggaaga ttttccacgt ctatctcatc
    14041 accaatatgc caattcaatt gcgcctgtag ccaagttttc ttattcggca gtcgtttgtg
    14101 caaagatgcg actggcaaca aatagatacc tgcttttttt tcttgaagtg cgttaagcgt
    14161 tgcaactctc tcgcctcgcg cttctggaga agaaaaggcc atttcagcag aaagcacttc
    14221 atccactgga aataaatgga tctgatcttg cggaatcaca tgttgcagat cttctgctaa
    14281 ctgattcgca taatacaaat ttggcatcag aataatcatg tttttatttg ttttcttaaa
    14341 cgctcgagca aatagaagtg ttttcgctga gcccgctaat cctgtgatta gttgtctagg
    14401 cattttttct tctaaattgc tgacccattc ttttacttgc ggatcttttc cgactaattg
    14461 aataatatcc attccttttc ctccttcctc aattgaatcg attcatcgca tccacgaacg
    14521 aatgcccttc tatgagatat tcgactgctt taacagattg acacatgctt tcttctatca
    14581 aaggctgatt ttctttgctg aaaggactaa gcacatgctg gactactgtt tttttccctt
    14641 ctggcctgcc gattcccacc ttgattcgat caaagacatt cgtattcaaa tgggaaataa
    14701 tacttttgat accgttatgc ccgccggcac ttcccttttg acggagccgg atttttccca
    14761 ctgccagatc caaatcatcg tagattacga ctaattcctc tggataaatc ccaaaataag
    14821 tcattagtgg gccgactgca cgaccagatt cattcatgaa tgtttgtggt ttgactaaaa
    14881 ggatcttttc tccattatga aaaaattcgg caacttctgc ttcgaacgga ttttttttga
    14941 aagtagcttg atgctcgcgt gcaattttat ccacgaccat aaagccgata ttgtgttttg
    15001 tatattggta tttggtacct gggtttccta acccgacgat cattttcatt tttgttttcc
    15061 acctaattct aaaaagcagc acaaaaggct ggactgctaa ttagcggtcc agtccatcga
    15121 gcttttttct tttttacact tgtcaatgca tagtataaca caaaacaagg cgtttcggct
    15181 aaaagagaat cgttaataaa tgataaagaa attaaaaatt tcttcaaagt ttaatgaaat
    15241 aattgatata taaaaatagt gaaataacgc acatgtaagc ttttacatat aatacaacct
    15301 tgttatctgt tatattttat agccaaatag cgtgacatgg ggcttgagaa atgttagcat
    15361 aagcatggga aaaaacaaat caatggaagg attgatacta aatgactgca gctgcagaaa
    15421 aaaaagatca tcaaaaagtt attcttgttg gagacggcgc cgtaggttct agctatgcct
    15481 ttgctctagt tactcaaaat atcgctcaag aagtaggtat tgtggatatc aatactgcta
    15541 aaactgaagg agacgcaatt gacttgtctc acgctttggc atttacttca cctaaaaaaa
    15601 tctattctgc tacttacgca gacgcacatg atgctgactt agttgttatt acagctggcg
    15661 caccacaaaa accaggtgaa actcgtttag acttagtaaa caaaaaccta aaaatcaacc
    15721 gcgatgttgt cacacaaatc gttgaatcag gatttaacgg tatcttttta gttgccgcta
    15781 acccagtaga tattttgact tactcaactt ggaaattctc aggtttccct aaagaacgcg
    15841 taatcggttc aggtacctct cttgactcag ctcgtttccg tcaagcactt gcggaattag
    15901 ttgacgttga tgcacgtaac gtccatgctt acatcttagg tgaacacgga gattcagaat
    15961 tcccagtttg gtcacatgca aatgttgccg gcttgcaaat ctatgaatgg gtaaaaaaca
    16021 acccagaaat cgatgaagaa gctatggtta acctattctt tggcgtacgt gacgctgctt
    16081 acacaattat cgagaaaaaa ggcgctactt tctatggtat cgcagtagca ttagcacgta
    16141 tcactcgtgc catcttagat gatgaaaatg ctgttcttcc tttatcagta ttcatgaatg
    16201 gcgaatatgg attgaacgac atctacattg gtgcacctgc tgttatcaac cgtcaaggta
    16261 tccaaaaagt aatcgaaatt cctttaagtg acagcgagca agaccgtatg gctgcttctg
    16321 ctaagcaatt aaaagaaatt ctagatgaag cttttgctaa actagacgct gaataatctt
    16381 tgaacatgac ggaaaacatc tattcggtgt tttccgtttt tttattttat cacccttatg
    16441 gtttctaaat tttttcataa agtagagata accttcgtct tttcctctaa attatgctat
    16501 catgagaccg ttccacccta aacttttact taaggagttg tcgatatgac aagatcttcg
    16561 catcgaaaaa aatcgaaaca tgttgctttg ctttcagttc ttggtgttct cttagtgctg
    16621 atcagctttt atacctatcg cagtacatac tacacaaagc attttttgcc gaatacagaa
    16681 ataaatggaa taaatgtcag caatctaaca gtcgaaaaag caaatgaaaa attaaaagaa
    16741 gcttattctg ataaaaaact ggctattaaa gaagatggaa aagtctggca ggaagtacca
    16801 aaatccgagc tcggctacaa agatgatttc acttcggagc tttctccgat cctaaatgaa
    16861 caaaacggat ggacttgggg aatgacttat gtctcagcgg cagaaaaaca agaaatagat
    16921 cctgtcagtc aaaaccacca aaaactagac actaccgttc agacattgac aactaagctg
    16981 actgatttga acaaagaccg gacacctact actgatgcaa cgatcgaaaa agcaggtgac
    17041 tctttcgtta tcaagcctga agtcaatgga aatacgatcg acgtcgatgc tgcggtcaaa
    17101 caattaaaaa gtgcggtaaa ctctggtaaa gatacaattg aattgactga attcaaagaa
    17161 aaaccaaaag taacatctga agacagctca ctgaaagaac aactcgcatc catgaatgct
    17221 atcgcaaatg taaaagctac ttacagtata aacggtgaga ctttccagat cccatcaagt
    17281 gatattatga gttggctgac ctacaatgat ggaaaagtcg atttagatac agaacaagta
    17341 cgtcaatatg taacagattt aggcacaaag tataacacaa gtacgaatga cacaaaattt
    17401 aaaagtacaa aaagaggaga agttacggtc ccagttggca cttacagttg gacgatccaa
    17461 acagacagtg agacagaagc attgaaaaaa gcgattctgg caggccaaga ttttactcgt
    17521 tcacccattg tgcaaggtgg cacaaccgct gatcatccat tgatcgaaga tacgtatatc
    17581 gaagttgatc tggagaacca gcatatgtgg tattacaaag acggaaaagt ggcccttgaa
    17641 acagatatcg tttccggaaa gccaactaca cctacacctg ctggagtctt ctatgtttgg
    17701 aataaagaag aagatgcaac tttaaaagga acaaatgacg atggaacacc ttacgagagt
    17761 ccggtaaact actggatgcc gattgactgg acaggtgttg gtatccacga ttcagactgg
    17821 caacctgaat acggtggaga cttatggaaa acccgcggtt cacacggctg catcaacacc
    17881 ccaccaagtg taatgaagga attattcggg atggtcgaaa aaggaacgcc tgtattggtc
    17941 ttctaacaaa a
//
LOCUS       DACDXO010000055        17818 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E23605_2017
            SAMN12639989-rid9881463.denovo.056, whole genome shotgun sequence.
ACCESSION   DACDXO010000055 DACDXO010000000
VERSION     DACDXO010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639989
            Sequence Read Archive: SRR10023389
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741914.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:42:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,972
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 33 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 17 of 73
            Pseudo Genes (multiple problems)  :: 10 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17818
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639989-rid9881463.denovo.056"
                     /isolate="E23605_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            123..935
                     /locus_tag="IWM35_002068"
     CDS             123..935
                     /locus_tag="IWM35_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HAP9919912.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            932..1732
                     /locus_tag="IWM35_002069"
     CDS             932..1732
                     /locus_tag="IWM35_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HAP9919913.1"
                     /translation="MKQRVVTAAVALVLFIPIIWIGGIAVELTAAILAVIGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            1893..3161
                     /gene="rseP"
                     /locus_tag="IWM35_002070"
     CDS             1893..3161
                     /gene="rseP"
                     /locus_tag="IWM35_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HAP9919914.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAILLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPDKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            3229..4938
                     /locus_tag="IWM35_002071"
     CDS             3229..4938
                     /locus_tag="IWM35_002071"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HAP9919915.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPERDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTNLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELVNTLPILLN
                     QGE"
     gene            5150..9508
                     /locus_tag="IWM35_002072"
     CDS             5150..9508
                     /locus_tag="IWM35_002072"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HAP9919916.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIH
                     IQLGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSF
                     IVKKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYA
                     PEGEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGK
                     KAGVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAV
                     KMYKGNVIESFDEFIDPGHPLSRTTVDLTGITDGMVRGSKSEEEVLRMFLEFSKDTIL
                     VAHNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSL
                     EQHHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILA
                     KNQAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKG
                     VEEARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVAT
                     GNVHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEI
                     VVENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRL
                     KKELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAP
                     HYYCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPD
                     IDLNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSA
                     EVDRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHS
                     IHDNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIY
                     SKTGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDAT
                     LAEVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVP
                     DWYIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMC
                     KGKDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFV
                     IEGDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGV
                     LKDLPDENQLSLFDML"
     gene            9653..10126
                     /gene="rimP"
                     /locus_tag="IWM35_002073"
     CDS             9653..10126
                     /gene="rimP"
                     /locus_tag="IWM35_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HAP9919917.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            10149..11324
                     /gene="nusA"
                     /locus_tag="IWM35_002074"
     CDS             10149..11324
                     /gene="nusA"
                     /locus_tag="IWM35_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HAP9919918.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNPKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            11346..11639
                     /locus_tag="IWM35_002075"
     CDS             11346..11639
                     /locus_tag="IWM35_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HAP9919919.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            11636..11947
                     /locus_tag="IWM35_002076"
     CDS             11636..11947
                     /locus_tag="IWM35_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HAP9919920.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            11960..14266
                     /gene="infB"
                     /locus_tag="IWM35_002077"
     CDS             11960..14266
                     /gene="infB"
                     /locus_tag="IWM35_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HAP9919921.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSNDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            14293..14640
                     /gene="rbfA"
                     /locus_tag="IWM35_002078"
     CDS             14293..14640
                     /gene="rbfA"
                     /locus_tag="IWM35_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HAP9919922.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKTAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
     gene            complement(14797..14970)
                     /locus_tag="IWM35_002079"
     CDS             complement(14797..14970)
                     /locus_tag="IWM35_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP9919923.1"
                     /translation="MIFLLTVNGPTNSNFYGAGSTDLLVTWLYLTVTKIDYNLASVIG
                     ILIFIYMQKFIYY"
     gene            15418..17780
                     /locus_tag="IWM35_002080"
                     /pseudo
     CDS             15418..17780
                     /locus_tag="IWM35_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
BASE COUNT         6087 a         3160 c         3911 g         4660 t
ORIGIN      
        1 ttaaaaaaag aaaaaattat tcagttttta aataaaaaaa caatgatttc ataggaaaaa
       61 cagttagata ttgctataat acttaagata gtgggaatct tttaagtata ggaggaaaaa
      121 gaatgttacg tttttttccg caaaaaaaca aatatattca agaaaaaagc gaatttcaat
      181 ttaataccga tggaccaatt cctcagcaca ttgccatcat catggacggc aatggtcgtt
      241 gggcgcaaaa ccgtcgtttg ccacgagttg ctggccataa agaaggcatg gaaacagtaa
      301 agaaagtcac aaaaaaagca tcaaggcttg gagtgaaagt gttgacgctt tatgcctttt
      361 cgactgaaaa ctggaagcga ccaaaagatg aagtaagttt tctcatgcaa ctacctgtcg
      421 acttctttga tacgttcgta ccagaattga ttaaagaaaa tgtcaaagtt catgtcatgg
      481 gttatgaaaa cgtattgcca gaacatacgc aagatgctgt aagacgtgca attgagcaaa
      541 caaaaaacaa tactggaatg gtattgaatt ttgctttgaa ttatggaagc agagcagaga
      601 tcgtgactgc tgtccaagaa attgccgaag aagtagcaaa aggagaaata cacgcagaag
      661 aaatcgatga tgaattgatt gctaagcatt taatgacagg tttcttgcct aaagagttac
      721 aagatcctga gctgatgatc cgtacaagtg gagaagagcg aattagtaat tttctgttat
      781 ggcagatcgc atatagtgag ctgtatttta caaaagcgct ttggcctgat tttgatggtg
      841 cgcatcttga agaagcaatc gcctcttatc aaaatcgaga ccgtcgattc ggcggtgtaa
      901 aaaaaacaga agatgagaaa ggggatcagt catgaaacaa cgtgtggtca cagcagccgt
      961 ggcgctagtc ttgtttattc cgattatatg gatcggaggc atagcggttg agcttacagc
     1021 agcaatctta gcagtaatag gagtttacga gctgtttcgg atgaaaggat tgacactctt
     1081 aagttttgaa ggagttttat ccgccgttgg tgcagtgtta cttgttttgc caaaagaaag
     1141 atggtttttc tttcttccag agaatgtaag tacatttatg ttattttatc ttatagttat
     1201 gattctttta ggaacatcag ttatttctaa aaatacgtat acgattgatg aagcaggttt
     1261 tcctgtgctt gtcagcttgt atgtgggaat aggtttccag aattttgtca atgcacgaac
     1321 agaaagcttg gctgttttga tatttggtct atttattgtt tgggcaactg atatcggagc
     1381 atatatggtt ggtcgacgtt atggtcagca taagttaagt cctgatattt cgcccaacaa
     1441 aacgattgaa ggtgcgctcg gtggtattgg tagtgctgtt gtagtcgcat ttctttattt
     1501 attgctatac ccagcaaaag aactatttaa tcatggtatg tttgtcatgc ttctgatgac
     1561 gattattttt tcagcagtcg gacaatttgg tgatttggtc gaatctgcaa tcaaacgtca
     1621 ttatgatgtg aaagactcgg gaaatatctt accaggccat ggtggaatct tagaccgttt
     1681 cgacagtttg ctgtttgtat ttcctatcat gcatttattt ggcattattt agtaagtata
     1741 cctgcgtttc tttgtgaatt tcataaaaga cgcaggtttt tatttttcat tatattgata
     1801 ctattggtgt ctttggtata ctatggtaaa attatagtgt tttatcgttg ttttataaaa
     1861 aacacaaaaa acaagtaaag gagaaaaaag ctatgaaaac gattctgaca tttatcatcg
     1921 tttttggtat attagtgatt gttcatgagt ttggtcattt cttctttgcg aaacgatcag
     1981 gaatcctcgt tcgcgaattt gctatcggta tgggtccgaa aatttatggg catcaagcaa
     2041 aagacgggac aacatatacg cttcgcctat tacctattgg cggttatgtt cgaatggcag
     2101 gcaatgggga tgatgaaaca gaaatggcac caggaatgcc tctttctctt ttactaaatt
     2161 ctgacggcat cgtagagaag ataaatttaa gtaagaaaat ccaattgaca aatgctatcc
     2221 cgatggaatt gagccgttat gatttagaag atgagctgac tatcactggt tatgtgaatg
     2281 gagacgaaac agaagtagta acctaccctg ttgatcacga tgcgacgatt attgaaaacg
     2341 acggaacaga aatcagaatt gcgccaaaag acgtacagtt ccaatcggct aaattgtggc
     2401 agcgtatgct gaccaacttt gctggaccca tgaataactt tattttggca atcctgttat
     2461 tcatcattct ggcatttatg caaggtggtg ttcaagtgac gaacactaat cgcgtaggag
     2521 agatcatgcc taatggagct gcggcagaag caggcttaaa agaaaatgat gaggtagtca
     2581 gtgtggacgg aaaagaaatc cattcatgga atgatctgac aacagtcatt acgaaaaatc
     2641 cggataaaac attagacttt aagatcgaac gagaaggtca ggtgcaatct gtcgacgtga
     2701 caccgaaatc tgtagagtca aatggcgaaa aagttggcca acttggtata aaagccccaa
     2761 tgaatacagg ttttatggat aagatcattg gagggacgcg tcaagctttc agcggttcat
     2821 tggaaatttt caaagcctta ggatcactat ttacaggttt cagtttagat aaattaggcg
     2881 gacctgtcat gatgtaccaa ttgtcttcag aagcagcaaa tcaaggtgta acaactgtta
     2941 ttagtttgat ggcgcttctt tcaatgaatc tcggaatcgt caatctgctt ccgattcctg
     3001 ccttagatgg cgggaaatta gtcttgaata tttttgaagg gattcgcggg aaaccattaa
     3061 gtcaagaaaa agaaggaata ttgacattag ctggctttgg atttttaatg ttgttgatgg
     3121 tgctagttac atggaacgat attcaacgat tctttttcta gaaaaagttg acaaataggg
     3181 tataataaca aacgataact attttgagga aataaaggag tatatagaat gagacaatca
     3241 aaaatgttga ttccaacatt acgcgaagtt ccaaatgatg cagaagtatt aagccatcaa
     3301 atcttgctaa gagctggata tatccgccaa gtatcagcgg gtatttattc atatctgcca
     3361 ttagcaaatc gtgtattgga aaaactgaaa cgcattatgc gggaagaatt tgaaaaaatc
     3421 ggagcggtag aaatgctgat gcctgccatc ttgccagcag aactttggga agaatccggg
     3481 cgttacgaaa catatggacc aaacttgtat cgtttcaaag accgcaatga tcgaaaaatg
     3541 atccttggac caactcacga agaaactttt actgatttga tgcgtaatga aatcaattca
     3601 tataagaaat tacctttaaa tctttatcaa atccaaccta aatatcgtga cgaaaaacga
     3661 cctcgcttcg gtttgcttcg cggaagagag tttatcatga aagatgcgta ttctttccat
     3721 gcaacggaag aaagtttgga cgagacatat aaagattacg aaaaagccta caccgaagta
     3781 ttcaagcgtt gtggcttaga atttcgctcg attatcggag acggtggcgc aatgggcggg
     3841 aaagactcaa aagaattcat ggcgatctca gaaatcgggg aggatacgat ctgttattcg
     3901 actgaaagtg attatgctgc aaacttggag atggctacaa gctactatgt atcaaaaaaa
     3961 tcgcatgaaa cacagcttga attggagaaa gtagctacac ctaacgtaaa atcaattgac
     4021 gaagtagcag atttcttttc tgttgaacct caaaaaatca tcaagtctgt attgttcatt
     4081 gcggatgaac aacctgtcct tgttttggta cgcggagacc atgaagtcaa tgacgtaaaa
     4141 ctgaaaaact ttttatctgc tgacttttta gaagaagcaa cagaagaaga agcacaaaaa
     4201 tatctaggtg ctgagtttgg ttctgtcgga ccagtcggtg tttcagatga cgtaaaagta
     4261 tatgcagacc gttatgtcca agatttagcg aatgccatta ctggagcgaa cgaaacagga
     4321 ttccactata aaaatgtgaa tccagaacgc gatttcactg tactttctta tgaagatctt
     4381 cgttttgtac aagaaggaga tccttcacca gacaacaatg gtgttcttgc ctttactcga
     4441 ggaatcgaaa tcggacatat cttcaaaatc ggcactcgtt acagtgaatc tatgggagca
     4501 actgtactag acgaaaatgg acgagaaaaa tttgtgatta tgggctgtta tggtatcggt
     4561 gtaagtcgtt tattatcagc aatcgtagaa caacattcag atgaacaagg aatcaactgg
     4621 ccaaaaggaa ttgcgccatt tgatttgcat atcgtccaaa tgaatctaaa agacgaatac
     4681 caaacaaacc tgacgaatga actagaagaa gcgatgacaa aagctggcta tgaagtactg
     4741 gttgatgaca gaaatgaacg cgcaggagtg aaattcgcgg attctgattt gattggctgt
     4801 ccaatcagaa tcactgtcgg taaaaaagca gtggatggaa tcgtagaagt gaaaatcaag
     4861 aaaactggcg aaatggttga agtacgtaaa gaagaattgg tgaatacttt accaatcctc
     4921 ttgaaccaag gcgaataata agcgttaaaa cacagaaaat aaaaatagct gaatcagctg
     4981 tttttgacaa tctgatgatt cgcttttaaa attggaactg cgatttgaat cgcagttcca
     5041 attttttaat gtaaaactca gaaaaaacga gtatactaaa aggtactgaa aaagaagagg
     5101 ataagatgcg atcaattgca ttgtttctca tcagaaaggg cgtatatctt tgtcagaaaa
     5161 acgagattta tttgaaaaac tgttggaaca aatccaacta gaggaagcag aaaaaaatca
     5221 tccactgctt actgctggtg aaatggatag tgtagtggtc catcgtaaat cgcgtttatg
     5281 ggaatttacg ctgcattttt ccaaaatttt gcctattatg ctatatcggt cattgactca
     5341 gcatctgact atcgctttta aagatattgc acaagtcaaa ctgaatatct tagcagatga
     5401 ccagacattc gatgaacagc tcttgcaaga ttactgggcc caagctttgg aaaatcagca
     5461 atgcgataca ccactagcac agcaagtatt gaaaacgcag gtacctgtga taaaagatcg
     5521 taaagtcatc ttgccaatcg acagtacagg agctatttct tatttgaagc aacaatactt
     5581 gccattagta gaagagttgt tcgtcagtta cggattccca aaatttcgta tcgaaccaga
     5641 agtggatgag cagcaagcgg aacgagtatt gaaacttttt gaagaacgca aacaagaaca
     5701 ggcagaagcc tttatgaagc aagcagctga aagtctagtt gtccacgaac aaaagaaaaa
     5761 ggaaagaaaa gagcaatcgc cagctcttga aggacccatc cacatccagc taggacgaaa
     5821 tattccagcc gatgaaccaa cgacaccaat gatcagtatc gttgaagaag aacgaagagt
     5881 cacacttgag gggtacgtat tcgacaaaga agtcagagaa ctacgttcaa aacgcaagat
     5941 cttgactttg aaaatcacag attatacttc atcatttatc gtcaaaaaat tctcgaacgg
     6001 cgaaaaagat gaacaagtat ttgatgcgat cagtgtcggt agctggttaa aggtacgagg
     6061 aagtattcaa gaagatacgt ttgtccgtga tttggtcatg aatgcgcaag atatcataga
     6121 agtcaaacat acacctcgca aagattatgc tcctgaaggg gaaaaacgag tagagcttca
     6181 tgttcatagc aatatgagta cgatggatgc aacaaacagc atctctgatc ttgtggcaca
     6241 agcagggaaa tggggacacc gagctatcgc cattacagac cacggtggtg cgcaagcctt
     6301 tccggaagca catagtgctg gaaaaaaagc aggtgtgaaa atcttatacg gcgtggaagc
     6361 gaatgtcgta gatgatggtg tcccaattgc ttataatgat gcacatgaag cattgagtga
     6421 agccacatat gtcgtatttg acgtggaaac gacaggattg tctgcagtat atgacacgat
     6481 catcgagcta gccgcagtca aaatgtataa aggaaatgtg atcgaaagct ttgatgaatt
     6541 tatcgatcca ggacatcctt tgtcacgaac aacggtagat ctgactggta tcacggatgg
     6601 aatggttcga ggatcaaagt cggaagaaga agtattgcgg atgttccttg aattttcaaa
     6661 agatacaatt ttggtagccc acaacgcggc ttttgatatg gggttcttga atactagcta
     6721 cgcaaggtat ggtattcctg aagcggccaa tccagtcatt gatacgttgg aattagctag
     6781 atatctgtat cctcaattca aacgtttcgg attaggtgtg ttatctaaaa aatttggtgt
     6841 cagcttggaa cagcatcacc gagcaatcta tgatgctgaa gcaactggtc acttagcatg
     6901 gatctttgtg aaagaggcaa tggacaatca taacatgctt tatcatgatc agctaaatga
     6961 gcatatcgga gaaggagatt cttacaaaag ggctcgacct ttccatgtaa cgattttagc
     7021 aaaaaatcag gcaggactga aagatttgtt caaactgatt tcaatgtcga atgtcgaata
     7081 cttcgaacgc gtcccacgaa taccacgctc ccaattgaaa aagatgcgtg aaaatctatt
     7141 gatcggctca gcttgtgata agggagaaat attcgaagcc atgatgcaaa aaggggtaga
     7201 agaggcaaga aaccgagcaa aattctacga ttatattgaa gttatgccaa aagcggtcta
     7261 tgcgcctttg atcgagcaag aattggtaaa aaatgaacat gatttggaag aaattatcca
     7321 aaatttggta gaaatcggaa aaagtttgga taaaatcgtt gttgcaacag gtaatgtaca
     7381 ttatctgaac gaagaagatg ccatttaccg aaaaattttg atcaattcta tgggcggagc
     7441 gaatccgttg aatcgtcata gtttgccgga tgtccatttc cgaacaaccg atgagatgct
     7501 aactgcattt cactttttag gggaagaaac agcgaaagag atcgtagtag aaaatacgaa
     7561 taaaatcgca gatatctgcg aagaagtgat tccagtcaaa gatgaacttt atacaccgaa
     7621 gattccaggt tcagaagatg aaatatcaga attgagttac acaaaagcaa aacaaatgta
     7681 cggagatccg ttacctgaga tcattcaaaa acgattaaaa aaagagttga attctattaa
     7741 tggtaatggc ttctcggtta tttatctgat cgcccaaaaa ctagtgcata aaagtaatga
     7801 agatggctat ctagtaggtt cacgtggctc tgttggctct agctttgttg cgacgatgac
     7861 aggaatcact gaagttaatc cgttagcccc tcattattat tgtccagagt gccaatattc
     7921 cgagtttttt gaagatggta cgtatggttc tggatttgac atgccagaaa aacaatgtcc
     7981 aaaatgtggt gcacgcttaa acaaagacgg tcatgatatt ccatttgaga catttttagg
     8041 attccatgga gacaaagtgc ccgatattga tttgaacttc tcaggtgatt atcaagcaga
     8101 agcccataac tatacgaaag tattgtttgg cgaagactat gtttaccgtg ccggaacgat
     8161 tggtacagtc gccgacaaaa cagcatatgg ctatgtaaaa ggatacgaaa gggacaataa
     8221 tctgcagttt cgaagtgcag aagtcgacag attagcaaaa ggcgctactg gagtcaaacg
     8281 gacaaccggc cagcatccag gggggatcat cgttattccc gattatatgg atgtctatga
     8341 ttttacgccg atccaatatc cagcagacga tcaaaattcg gaatggaaaa cgactcattt
     8401 tgattttcac tcgattcacg ataatgtatt gaaattagat attctagggc atgatgaccc
     8461 aactgttatt cggatgcttc aagatttatc agggatcgat ccacaaacga tccctacgga
     8521 tgacccggaa gtgatgcgga tatttgccgg tcctgaagta cttggagtaa gtcaggaaca
     8581 aatttattcc aaaacaggga cattaggtat tccagaattc ggcactcgtt ttgttcgagg
     8641 gatgctagaa gaaacgcatc ctacaacttt tgcagaacta ctgcaaattt ccggactatc
     8701 tcatggtaca gacgtatggt taggaaatgc ggaagaattg attcgacgag gagatgcgac
     8761 actggcggaa gtaatcggct gtcgtgatga tattatggtt tatttgatcc atgctggatt
     8821 agatagcggg atggcattta aaatcatgga aactgttcgt aaaggacaat ggaacaagat
     8881 ccctgatgaa ttgcgagaaa cctatctatc ggcgatgaaa gaaaataatg tgcccgactg
     8941 gtatatcgat tcttgttcaa aaatcaaata tatgtttccg aaagcccatg ctgcagctta
     9001 cgtattgatg gctcttcgtg tcgcttattt caaggtttat ttccctattt tgtattactg
     9061 tgcatacttt tctgtacgtg cagacgactt tgatctagtc tccatgtgca aaggaaaaga
     9121 tgctgtgaaa caagcaatga aagaaatcac ggacaaagga ttagatgctt ccgtaaaaga
     9181 aaagaatcaa ctgacagtgc tggagttagc caatgaaatg ctggaacgag gattcaaatt
     9241 tggaatgatt gatttgtata aatcggatgc cgttaacttt gttattgaag gtgatacgtt
     9301 gatcgcacca tttagagctg tcccaagttt gggaaccaac gtggcaaaac aaatcgttga
     9361 agcaagaaaa gacggtccat ttttatccaa agaagatctt gccacccgcg gtaaagtttc
     9421 aaaaacattg atcgaatata tgaacgacaa tggcgtattg aaagatctac cagatgaaaa
     9481 tcaattatcg ttattcgata tgctataagc atctgttttc ctgtctgctt gtaattttaa
     9541 gcagagtatg gtatagttct tataggtaat gatactaact gtgagtgagc ggatttttcg
     9601 ctcactcttt ttaatggaat tacgcactta attttttaag gaggcgaaac ttttgagtag
     9661 cgtcgttgaa acagtcacag aaatggtgac accgatttta gatgaacaaa attttgaact
     9721 tgtcgaagtc gaatttgtaa aagaaggaaa aagctggttc ttacgagtat ttattgacaa
     9781 agaaggcggt atcgatatcg aagaatgcgc ctttgtcagt gagaaactga gtgaaaaact
     9841 tgatgcagca gatccagatc cgattcctca agcttatttc ctggaggtat cctccccggg
     9901 agcagagcgg ccattgaaaa aagaaagcga ctatgaaaag gcacttggtg attatgtcca
     9961 tgtctcctta taccaaccag tagatggcga aaaacaatat gaaggatttc ttcaatcatt
    10021 tgatgctgaa cagcttacat tgaaaatacg cataaaaaca cgagaaaaag aattgacttt
    10081 tgaccgaaaa aatattgcaa aagctcgctt agctatccaa ttttaatcac ggagaggaat
    10141 cagaaaaaat gagtaaagaa atgttaaatg cgttagacgc tttagaagct gaaaaaggga
    10201 tctcaaaaga aatcgtgatc gatgctttag aagcagcatt ggtttctgca tacaaacgtc
    10261 actatggcca agcacaaaac gtcgaagtgg aatttgatca gaaaaaagga aagatccatg
    10321 tctatgcggt aaaagaagta acggaagaag tgatggattc acagttggaa gtctcattga
    10381 aagatgcact tttaatcaat ccagcatatg agatcggtga tacgatccgt tttgaagtaa
    10441 caccaaaaga ttttgggcgg atcgctgcgc aaacggctaa acaagttatt ttacagcgtg
    10501 tacgtgaagc agaaagaacg attatttata acgaattcag tgcttatgaa aaagacatca
    10561 tgcaaggaat cgtcgaacgc caggataaac gttatattta tgtcaatctt ggtaaaattg
    10621 aggcagttct ttctaaacaa gatcaaatgc caaatgaatt ttatcaacca catgatcgaa
    10681 tcaaagttta tgtttcccga gtagaaaata cgtctaaagg tccgcaagta tttgttagcc
    10741 gcagccatcc agacctattg cgccgcttat ttgagcaaga agtaccagaa gtctatgatg
    10801 gaatcgttga aattgtcagc atcgcccgtg aagctggcga tcgttcaaaa gtggcagttc
    10861 gctcaaatga tccaaatatc gatgcagtcg gtacatgtgt cgggccaaag ggacaacgag
    10921 ttcaagcaat cgtcaatgaa ttaaaaggcg aaaacatgga tatcgtcgaa tggaatgaag
    10981 atccagctgt attcattgca aatgcattga atcctgcaca agtcatggat gtgatcttcg
    11041 atgagacaaa tccaaaggcc tgcacagttg tcgttccaga ctaccaatta tcgctagcaa
    11101 tcggtaaacg cggacaaaac gcacgtttag ctgctaaatt gactaaccat aaaatcgata
    11161 tcaaatcaga atctgacatg acggaatttt atgaaaatca agaacaacaa aaagaaacag
    11221 aagaactgca tgatgaagca atcgttcagt cagatttaac agacgatgaa tatgaaacaa
    11281 ttgcattcaa caatgaaacg gtcgaagaaa aaccagaagc ttaactttag taaggaggca
    11341 aagagatgaa acaaaggaaa attcctttac gcaaatccgt ggtttcaggt gaaatgaaac
    11401 ctaaaaaaga gatggtccga atcacccgtt caaaagaagg ggaagtatcg atcgatccaa
    11461 ctggaaaaat gcctggacga ggagcgtatg tctcacttga gccggaagaa gtacaacaag
    11521 cttgggataa aaaaattctc gatcgagtac tcgaaacaac attaacagat gagttttatc
    11581 aagaacttct tgactatgtc actcaccaaa aagcacgtaa agagttattt ggaaaatgaa
    11641 tacagttaat cggcaaaaag ccatgaatct catcggtcta gcgatgcgcg cgggaaaact
    11701 aatcacgggt gaagaactga ccattgctga aatccgcaaa caaaaagcaa aattggtgtt
    11761 tgtggcaacc gatgcaagcg agaatacaag aaaaaagata aaagataaga gttcatatta
    11821 tgaagttcct tgctttgagc tattttctga agcagaaatc acgcagatga tcggaaaacc
    11881 tcgcaaggta ttcggaataa tggatgccgg atttgcgaag aagaccaagg agctaattga
    11941 aggttaggaa ggtgattgca tgggcaacaa aagaatctat gaattagcaa aagaactcaa
    12001 aaaatctagt aaagacgtcg tggaaaaagc acaacaactt ggcatcgatg tgaaaaacca
    12061 tatgggtgcg attactagca atgatgagaa gaaattgcaa caagcgttca aaaacccaag
    12121 cacgaaacaa caagctgcac aacaagcaag cagaaaacca gccaatcaac agccgaacga
    12181 tcaacgtaaa aaagaaacaa cgaacaatca acaaaaaaat aaaaataacc gcagttatca
    12241 agatcgcggt caagggagcg gtcaagtgaa tcaaggaaaa aaaccaacaa caaaccaaaa
    12301 taatacggga aataccagca gccaaaatcg tcaaggaacg agccaaggcg gcaatcaaaa
    12361 ccgtactaac aataacaaca aaccaaacaa taacaatcgc ggcaaattta ataataataa
    12421 ccgtaatcgt tataataaaa aaggtaaaaa aggcaaacag caacaatcaa ctaaaccagc
    12481 tgtaccacca cgcaaattcc gtgaattgcc agatgtatta gagtatactg aaggtatgaa
    12541 cgtagcagat attgcgaaaa aaattcatcg tgagccagca gaaatcatta aaaaattatt
    12601 tatgcttggt gtcatggtca atcaaaacca agcactggat aaagatacga ttgaactgtt
    12661 agcgacggac tatggcatgg aaccacaaga aaaagtccaa gttgacattg ctgatatcga
    12721 taaattcttc gaggcagaag aagtcgatcc agataaatta gtctcacgtc cgccagtcgt
    12781 aacgattatg ggacacgtag accatgggaa aacaacttta ttggatacat tacgccattc
    12841 acgtgtaaca agcggcgaag ccggcggaat cacacagcat atcggtgcct accagatcga
    12901 tattgatggt aaaccaatca ctttcttgga tacaccagga catgcggcct ttacaagtat
    12961 gcgtgcacgt ggagcaagta tcacagatat cacgatctta gttgttgctg cagatgatgg
    13021 cgtcatgcca caaacagttg aagcaatcaa ccatgccaaa gctgcaggcg ttccaatcat
    13081 cgtcgctgta aataaaattg ataaaccagg tgcaaaccct caacatgtta tgcaagaatt
    13141 aagtgagtat gagttaattc ctgaagcatg gggtggagaa acgatctttg ttgagatctc
    13201 agcgaaattc ggccaaaaca tcgaagaatt gctggaaatg atcctgttag tcgcagaagt
    13261 agaagattta aaagcagatc caacacaacg cgcaatcgga acagtgatcg aagcacgtct
    13321 tgataaaggt aaaggtcctg tgtcaacctt acttgtccaa gaaggaacac ttcatgttgg
    13381 tgacccaatc gttgtcggta atacttacgg acgtgtccgt gtaatgacaa acgatcttgg
    13441 ccgtcgcgat aaagaagcag gacctgcaac gccagtagaa atcactggct tgaatgatgt
    13501 acctcaagca ggtgaccgtt tcgtagtctt cgaagacgaa aagacagcac gtgcagcggg
    13561 tgaagaacgc gcgaaacgtg cattattaga aaaccgtgct gccacaagcc gtgtgacttt
    13621 ggataactta tttgaaagcc tgaaagaagg cgaattgaaa gaagttaacg taatcatcaa
    13681 agcagatgta caaggttcag cagaagcact ggctgcatca ttgaagaaga ttgatgtgga
    13741 aggtgtccgt gtaaaaatcg tccattcagc tgtcggagca atcaatgaaa gtgatgtaac
    13801 cttagcagca gccagcaacg ctattatcat cggtttcaac gttcgtccta caccgcaggc
    13861 aaaagtgcaa gctgatactg aagaagtcga tattcgtctt caccgtatca tctataaagc
    13921 catcgaagaa atcgaaactg cgatgaaagg gatgctagac cctgaatttg aagaaaaaat
    13981 caccggtcaa atgatcgttc gtgaaacctt caaagtttct aaagttggta cgattgcagg
    14041 tgcctatgta acagaaggat atattcgtag agatagcggt gtccgtgtaa tccgtgacgg
    14101 aatcgtaatc tatgaaggcc aacttgcaag tctgaaacga ttcaaagatg acgtgaaaga
    14161 agtcaaaatg ggttatgaat gtggtgcaat gattgaaaaa ttcaatgaca tcaaagtaga
    14221 tgacgtaatt gaaggattta tcatggaaga agtcaaaaat gactaaaaac tgataaaagg
    14281 aggccaatca ttatggctaa ctaccgtgac cgccgagtcg gtcaagaaat cttaaaagaa
    14341 gtcaatgata ttttgcgcaa aaaggtccgt gaccctcgtg tggagaacgt aacgatcaca
    14401 gatgtgaaag ttacggggga cttacagcaa gcaacgatct attacagcat tttatctgac
    14461 ttagcatcag ataagaaaac agcacaagat ggattgaata aagcatcagg attgattcgt
    14521 cgagagttag ggcacaatct aagtatttat aagacacctg aattgttctt tgaacttgat
    14581 gaatcagttg tctatggcaa ccatatcgat gaattgcttc gcgacttgaa caaagactaa
    14641 tcaaaaactc ccgcaaatcc tgtgaaatca ggggttgcgg gatttttttg ttttcattaa
    14701 gacgtattac ctaagagttc catgacttta gtcttgcggc gtgtcagcta accaaaaaag
    14761 agaaaaagaa aagtaaaaaa ttcgactttc ttttttctag tagtatataa atttttgcat
    14821 atagataaag atcaagatac cgataacgga agctagattg taatctattt tagtgacagt
    14881 caggtatagc caagtaacta gaagatcagt ggaacctgca ccataaaagt ttgagtttgt
    14941 cggtccgttg acagttaaaa gaaaaatcac gttgaagtta ttgatatttc cgattacgcc
    15001 gcttcgattt ggtcacgatc caaattttgg ataatccctg tagaaattag catagtagct
    15061 gggataccaa ttcacatatt gacaaagata acagagactt ttacataaaa cggatcaatc
    15121 aggaaaggaa ttgctcgatc cagccccagt tttccagcaa tgtgttaact ggcccagcgt
    15181 cattcagcaa attatgcatg atgaggagcg aaatgaactg cggaacagcc attgtgataa
    15241 cgacaagttt tttggatcaa tgtaaaaaac gtcagcgaaa ggaaaaattt cgtggttttt
    15301 tatacctgtt catttggcta gatataaaat ccaaacaaat gaacagaagt gcttattagg
    15361 tttcaattgg gagtatctgc gctagaataa gagtaataat gaaaaggagt gttatgtatg
    15421 gattattcta gtaaagaata ctttgataaa atgacggctt ggtggcgtgc tgctaattat
    15481 ctttctgttg ggcaacttta tcttaaagat aaccccttac tgagacgtac actgaaaccc
    15541 gaagatgtca aaaaacatcc aatcggtcat tggggaacga ttcctggcca aaattttatt
    15601 tatgtccatt tgaaccgagt catcaataaa tatgatttga acatgtttta tattgaaggg
    15661 ccaggacacg gcggacaagt catggtatcg aatgcttatt tagatggaag ttacacagag
    15721 atctatccag aagtaacgga agatgagaca ggaatgcaaa aattattcaa acgattttca
    15781 tttcctggag gcattgcttc tcatgcagca ccagaaacac caggttctat ccatgaaggc
    15841 ggagagcttg gatactctct ttcacatgca gtcggagctg tattagacaa tcctgaagta
    15901 atctctgctg tcgtgattgg tgatggtgaa gcagaaaccg gtcctttagc tggaagctgg
    15961 ttctcaaatg tctttatcaa tccagtgata gatggagctg ttttaccgat ccttcattta
    16021 aatggtgcaa aaatagccaa cccaacgatc ttggcacgta aatcagatgg agaattagca
    16081 aattacttta acggacttgg ctgggaacct ttctttattg aaggaaatga ttcagaaaaa
    16141 ttaaatcctg ttatggctga aaaaatggat caagcaatcg agaaaattaa atccatccaa
    16201 aaagaagctc gcctgaagac agcaacagat gtcgtgatgc caaaatggcc agtattgatc
    16261 gtacgcacac caaaaggatg gaccggtcca aaagaatggg acggggaacc aattgaagga
    16321 accttccgag cacaccaagt accgattcct gtagatcaag aacatatgga tcatgcagat
    16381 gctttactcc gttggctgaa atcctatgaa ccagaaaaat tatttgatgc acaaggacgt
    16441 attttagaag aaatcagaga aatcgcgcct actggtgacc aaagaatggc aaaaaatcct
    16501 atcaccaatg gtggaatcga tccaagaccg cttattatgc cagattggaa aaaatatact
    16561 ttgcaatttg aaaaaccagg ctccgttaaa gcagaagata tgaccgaact tggtaaattc
    16621 gttcgcgaga tcattgagaa aaatccagaa aatttccgta tatttggacc agatgaaaca
    16681 aaatcaaacc gattgaatca agttttcaaa acgacaaatc gccaatggat ggaaaaaatc
    16741 gaaccagaaa atgatgaatg gctttcgcca tctggtcgcg tcatcgacag tcaattatcg
    16801 gaacaccaag atgaagggtt cttagaaggg tacgttctga ctggacggca tggttttttg
    16861 caagttacga atcattcttg cgagtggttg actctatgtt gactcagcac ttcaaatgga
    16921 tgcggaaatc acatgacctt tcttggcgaa atgattatcc ttcgctaaat ctcattgcaa
    16981 gctcaacagt cttccagcaa gatcacaatg gatatagcca ccaagatcca ggaatattga
    17041 cacatttggc agaaaagaag gcggagttca tccgtgaata tctgcctgcg gatgcaaata
    17101 cattgctcgc agtcatggat aaagcattcc gctcgtcaga aaaaatcaat ttgatcatct
    17161 cttccaaaca tccgcgtgct caattttact cagcagaaga agcagccgtc ttagtgaatg
    17221 aaggcttgaa aatcattgat tgggcttcga cagcaaaaga ggaggaacct gaattggtca
    17281 ttgctgctgc aggaacggaa tctaacttag aagcattagc agctgtaact ttgctgctgg
    17341 aagaattccc aaaactaaaa attcggttca tcaatgtcgt agatttattg aaattacgtc
    17401 atccaagtca agatccgcgt gggttgagcg atgaagaatt tgatcaatat tttacaaaag
    17461 acaaaccgat cttatttgct ttccacggtt atgaaacatt ggtccgcacg attttctttg
    17521 atcgtcataa ccatcattta atgattcatg gctacaaaga aaatggagat atcacgacac
    17581 catttgacat gcgtgtagta aatgaacttg atcgttatca tttggcaaaa gacgcagctt
    17641 taaaaatcaa aggaagtcaa gcagaagatt ttgcgaaaaa aatggatcag aaattacaag
    17701 aacatcaaaa ctatattcgt gaaaatggta ttgatctgcc agaagtattg gattggaaat
    17761 ggaaaaatct tgaccaataa aatgataaaa tcgatcccgc aaaatagaat tttttaca
//
LOCUS       DACDXO010000056        17457 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E23605_2017
            SAMN12639989-rid9881463.denovo.057, whole genome shotgun sequence.
ACCESSION   DACDXO010000056 DACDXO010000000
VERSION     DACDXO010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639989
            Sequence Read Archive: SRR10023389
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741914.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:42:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,972
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 33 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 17 of 73
            Pseudo Genes (multiple problems)  :: 10 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17457
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639989-rid9881463.denovo.057"
                     /isolate="E23605_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            76..1017
                     /locus_tag="IWM35_002081"
     CDS             76..1017
                     /locus_tag="IWM35_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HAP9919924.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            complement(1167..2063)
                     /locus_tag="IWM35_002082"
     CDS             complement(1167..2063)
                     /locus_tag="IWM35_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HAP9919925.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            2332..3672
                     /locus_tag="IWM35_002083"
     CDS             2332..3672
                     /locus_tag="IWM35_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP9919926.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            3890..4333
                     /locus_tag="IWM35_002084"
     CDS             3890..4333
                     /locus_tag="IWM35_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HAP9919927.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(4837..6255)
                     /gene="nhaC"
                     /locus_tag="IWM35_002085"
     CDS             complement(4837..6255)
                     /gene="nhaC"
                     /locus_tag="IWM35_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HAP9919928.1"
                     /translation="MGKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKRYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDVLEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPNLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(6658..7881)
                     /locus_tag="IWM35_002086"
     CDS             complement(6658..7881)
                     /locus_tag="IWM35_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HAP9919929.1"
                     /translation="MTTTISTNQLKDLIQYRLPEYQALALDIHEHPEVSNYEFYSSAA
                     LIKQLEKERFWVEKEVAGHRTAFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDETGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDVALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAAISTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSDKGLASIAIAAELLANTALDLLKEPKLLERIKQE
                     HQEIVARKKQEGATF"
     gene            complement(7912..8751)
                     /locus_tag="IWM35_002087"
     CDS             complement(7912..8751)
                     /locus_tag="IWM35_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HAP9919930.1"
                     /translation="MKKIIRFVVITFVAIIWLAGCSQGKADTTDKKETIVKLGIIGED
                     TDVWDNVKDRLKKENINLEYVKFTDYNQPNVALADGSIDLNSFQHQFFLDNYNKEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENRKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDDHARPYVNIIVARKEDENNKTYQKIVDAYQTEDTVKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(8768..9448)
                     /locus_tag="IWM35_002088"
     CDS             complement(8768..9448)
                     /locus_tag="IWM35_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9919931.1"
                     /translation="MTDFFKTYLPNVYLIPDEFVEATKQTLYMSFWTAFIGGMIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILILVFLSQFISN
                     QLIKKITH"
     gene            complement(9450..10523)
                     /locus_tag="IWM35_002089"
     CDS             complement(9450..10523)
                     /locus_tag="IWM35_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP9919932.1"
                     /translation="MIELRDVSVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEDGR
                     IVEKGSSIQIFSNPEEELTKDFIRTATHLDQALETIIAHSAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRKKALDYLIKS
                     GVKINILQKNDLDSRIKVIEGGR"
     gene            complement(11170..11841)
                     /locus_tag="IWM35_002090"
     CDS             complement(11170..11841)
                     /locus_tag="IWM35_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP9919933.1"
                     /translation="MKGAIFDLDGLLVDTEKTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVKAAGSPEKVQEVRAKREEYFYQQLTSGAIHLKPYAKEILADLKERHM
                     KLGIATTTVTKRATDILDHFKLADYLDTFTFGDEVSENKPSPIPYLTALERTGLEASS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYTVEEKEKLKLLAEGKDLRTVIEMLN
                     KKTEK"
     gene            complement(11928..14606)
                     /gene="mgtA"
                     /locus_tag="IWM35_002091"
     CDS             complement(11928..14606)
                     /gene="mgtA"
                     /locus_tag="IWM35_002091"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HAP9919934.1"
                     /translation="MSFGHKGEIEMEKKKDTQKIYAAFAGKTKQELFELFHASANGLT
                     EEQIERSRENYGENAISYGKKTPFIVEILKAYITPFTLVLIALGIISFITEYVLAAPG
                     DKDLFGVAIIFTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGKYTEIPMEE
                     IVCGDLVRLSAGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSY
                     ENLVFMGSNVISGSAEGIIVSVGNETLFGHVAETLSEKPIQSSFELGIHKTSMLLIKF
                     MSLMAPAVIVINGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGT
                     VIKNLNAIQNFGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQT
                     GLKNLLDVAIIDEATETLDTDKINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGA
                     IEEMLNISKFIDLGGTVTPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFS
                     VKDESDMVLIGYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLE
                     INELVTGEKISEMSDHELAQVAEDHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGIN
                     DAPAMKVADIGISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKAT
                     ASSNFGNMFSVLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKW
                     NASSIGEFMVYFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQRVIFIALFHAG
                     WFVESLWTQTLVLHALRTPKVPFIQSNATFAMFVITTLGIIVGSVLPFTSFGAELGLM
                     PLPGNYWLWLFATVIAYLALVTAVKKIYIRRFQELL"
     gene            15265..15702
                     /gene="ndk"
                     /locus_tag="IWM35_002092"
     CDS             15265..15702
                     /gene="ndk"
                     /locus_tag="IWM35_002092"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HAP9919935.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFETMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKAADAVPGTIR
                     GDYALPGTENIIHASDSRDAAVKEIARFFPPFDINKPSEKAAK"
     gene            complement(15724..16590)
                     /locus_tag="IWM35_002093"
     CDS             complement(15724..16590)
                     /locus_tag="IWM35_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP9919936.1"
                     /translation="MYDGELIKKLRVNKKLTQSQLAEGICFKTSLVGIETNSVKKMSF
                     VTLRSFLERMNMTLAEYEWMRNQLDEPIKLKKKRRMLDRVQEDSFDPYKEISNNRRHF
                     KKTEDLYYLVLNLHMVEKTEVSETEFEFLRYECRIIEEYLMQIPDYGQFELSILADFP
                     WIFSDRFIENNYLKIKKRMRRLAQLNNHEQYLFTFLMNLTSFYIENGRLKKARSVNED
                     MKRSLAHKERSTVIYETLMADYYQRLISAALGEEKTKDYQEFFTVLEYMLGKNQRRSL
                     EKKLLAIAEPSN"
     gene            complement(16750..>17457)
                     /locus_tag="IWM35_002094"
     CDS             complement(16750..>17457)
                     /locus_tag="IWM35_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HAP9919937.1"
                     /translation="YLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEF
                     DNAQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQ
                     HDQVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLS
                     DKLNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTT
                     NLYPVTRTDVEQQKNNQ"
BASE COUNT         5077 a         3577 c         3090 g         5713 t
ORIGIN      
        1 aaacaaaaca atgctatttt cttccatttt tctatatact aaaagggaac tcattgaata
       61 aaggagagaa aaaacatgaa aatcgcaata gctggggccg gagcaatggg cagccgaatc
      121 ggattgatgc tacatcaaag cggaaatgag gtcctcttga tcgatcgttg gcctgctcac
      181 attgaagcca ttcgtacaaa tgggttgatt gctgacttta acggtaaaga agtggtcgca
      241 aaactgccaa tctattcacc agaagaaatc atcgaaagca acgaacacgt ggacttgatc
      301 gtagcattaa caaaagccaa tcaattagat gatatgtttt gttcgatcca atccattatt
      361 actgacaata cttatgtttt gtgtttacta aatggattag gtcatgaaga tgtactggaa
      421 aaatatgtgc caaagaaaaa tatcttattt gggatcacga tgtggacagc tggtctagct
      481 ggtcccggaa aagtcacatt gttaggtgat ggagaaatcg aactagagaa cttggaacca
      541 gaaggcgaag catttacaaa aaaagttgta gaagtctttc aagaagcaaa tttaaatccg
      601 atctatagcc ataacgttcg ttattccatt tggcgaaaag cttgtgtaaa tggaacattg
      661 aatggtttat gcacgatttt ggattgtaat attgctgaac taggcgcgca aaaagcggca
      721 gagagcatgg ttcgaacaat cgtatctgaa tttgcttcta tcgcagcaaa agaaggaatc
      781 atactagatc aagaagaagt gtaccaacat attgcttcta cttatgatcc tgacaacatc
      841 gggttgcatt acccatctat gtaccaagac ctgatcaaaa accatcgttt gactgaaatc
      901 gactacatca atggtgccat ttggcgaaaa ggacaaaaat atgatattgc tacaccatat
      961 tgtgcctttt tgacccaatt agtccatgca aaagaaggaa tccttggcgc agaataaaag
     1021 gctctctgat gagaatcagc tgaatcagca gctggttctc tttttttaag cccaataaaa
     1081 gatcaaaaag aaaacacaac tctcatgatg ccataagaat tgtgcttgtt tgtttcagta
     1141 cataatctct tcaaaatgta ggttttttat ttgttttcaa cagaggagag gtcatattcg
     1201 tattgctcat aaatagtgga atcgttagtg ataaatcgtt ttgcccaatt ttctgcatca
     1261 taaaattcca atagctctga taggattggc ttaaatggtg tatgcagaaa ttccggtaat
     1321 tcatctaaat cacgttctcg aagcaaaaaa atattttcag gacgataaaa atgataatga
     1381 gcaatcgctt gattatccgt cactagtttc ttttgaaaat acatggattc aaatgtccgc
     1441 aaagtcactc cttgctgacc ctcccgcaac agttccagaa tcccgcgcga ttcccctgtt
     1501 tttttcaggt actcttcata agtaagatat ccaggttgtt cattccctcg atgtggaaga
     1561 atgaaatagt tggtcgtaac atctcttttt ataaattcct gcttatagct ttctgctcgt
     1621 tcttttctcc cattattcgt tgcaccaaaa aatagatccg tctttggttc gctagaagaa
     1681 aaattcatat gtttcgaata aaatgaacta ttatgtttca gcccaagttc ttttgcttcc
     1741 aaaaaatcaa aatgataaat ctcatcgatt gccggttcat cttgtgcctg cttcagcatc
     1801 gcctcgtgtt ctgctttgaa gtgattccaa aaaaataaca caatttttgc attggtaaat
     1861 gggcgcacgc gtcgagccat ctccatatca aatccataat ctaaaaaaac gatcagctga
     1921 taatttgcca actgttgtct agctgattta atgaattccg actgtagtaa atcttttccg
     1981 gaccaaacat ctaaagagcg gatggaaggc aaaaaataaa gtgatttatt ttttgcaact
     2041 agcagtacgt catttttgtt catcttttcc gcctcacttt ctctgtcact ttttcctatg
     2101 atagcctgtt taaatagtaa aaagccagta aaacgtttcc tatatttgta tgaaaattta
     2161 acatttgtat gaaaatatga tgaaataaat cattcttcta ttaaaattcg cctaaaagtt
     2221 ttctgaatag ttgtaattgt ctgaaaattt gttatcatta gtcattgtta aatattgcta
     2281 tttattctta atataaattt tgattgtgaa tttaaaagaa agaggtcatt tatggaagat
     2341 aaaaaattga aaaaacaact tagctccaga catatcacca tgttagcttt aggtggagca
     2401 atcggtgcag gtctatttaa aggaagtggc gaagcgattg ggatcgccgg cccttctgta
     2461 ttgattgctt ttttgattgg tgggctgatt ttatatatcg tgatgaaagg tctgggaaaa
     2521 atcgttttaa gcggtggcga cacgcatcac ggattatctg gtttgatacg cccttatcta
     2581 ggtgctcatt ctgctgattt tactgattgg gtctactggt ctatgtggat tatcaatatc
     2641 atcgctgaag ctgttgcagc tgcttccttt ttgcagttgt ggttcccgca cattccagcc
     2701 tgggtatttg tttttatgtt ggctgtttta actacgatca tcaatcttta ttcggtacgg
     2761 ttattcgcag aaacagaata ttggctggct tttgctaaaa tcagtgtcat tatcttactg
     2821 atcatttttg ctacttatct tgtcggtcag caaatgttgg gcacaggcgt tttccctacc
     2881 cttcagcaat tgactgacca tggtgggttt actcctcatg gtatgaaggg aatcatcagt
     2941 tctttgcttg tagttatcta ttcttacggc ggttctgaac tgattgctat cacggtaagc
     3001 gaagcagatg atcctaaacg tgcgattcct aaagcaatca aaggtgtcat gggaagaatc
     3061 atttcctttt acatcattcc tttgtttttg cttttgattc tttttccttg gaatacttta
     3121 gcaggcacga atgtcagtcc ttttgtgatg gtatttgaaa aaatgaatat cccatttgca
     3181 gcggatatcg tcaattttgt gattgtcctt gctttgttct catctatcaa ttcgggtgtg
     3241 tatgcatctt caagaatttt gttcttccgt ttgaaggatc gaaaaggttc ctctagaaaa
     3301 ctagctgttt taaacaagca tcaagtaccg caacgtgctg ttttattttg taccagtgtt
     3361 ctttatcttg gtgtagctct ttcttatttt gtcggtgaca aattatttgg ctatctggca
     3421 ggttcccttt cgtatacagt attattgatc tggatcatta tcagtgcggc atcctttgta
     3481 ctggcactaa gaaaaggaac attatgggat aaaggaatca gcttttttgc attagctgtc
     3541 ttaggattga ttttccttgg tatcctgttc acgaactcca ttggggtaac tgtattgact
     3601 ggcctgcttt acctgttcat ttatttcagt tatcaaaaaa agaatgatgc gttcgtattg
     3661 acgaatgaat gaaatcgata gaaaatttta ttttatttat gtggaataga tggagctatt
     3721 gaagaaagga gttgtgacaa aagtcttgtt gcaactcctt tttccgaata aacagtctca
     3781 tgacctcttg gtaaacggtg ttcaaaaacc ctctgtgcaa aataattgat tgttctccat
     3841 gctcatgaca tgatagaaga aaggaatgaa aaaaaggagt ttgttttaaa tgaaaaagta
     3901 ttggaagttt ttgttgatga tcggtgtttc aacggtagtt atgtttggta tgatgtactt
     3961 gaatgtctat gcgctcgatc atatcttctt cagtcgtaca cgagtattca tggcgttgat
     4021 gatgggtgca gtcatggcag tcatcatgct gttgttcatg tggaaaatgt atgaaaacaa
     4081 aaagataaat gttatgatct tagccgctag tcttcttgtt tttgccggtt ccttgtttat
     4141 ggtacgaagc caaactactg tcaacgacac agcttggatg aaggccatga ttcctcatca
     4201 ttctatcgct atcctggcaa gtaaacgagc tgatttaaaa gatcctgaag taaaaaaatt
     4261 agcggaagaa atcattcagg cacaagaaga agagatcgac aaaatgaaaa aaatgatcaa
     4321 agaaatggaa tgaccattat ccgtccaaat aaaaaaagag gatgttccag aagttatcag
     4381 cttcaagaaa taagaagaag ctacttctgg aacgccgtga ataaggggtt gtgaaaaagc
     4441 tgccctgcat caagtaataa gaacagccaa acaaaaatag cttctcctat ttttcgtttg
     4501 gttaggctta ttaccgcaga tgcaagcttt tgaacaccgt ttattaagag gttgtaaaat
     4561 cagcgttctg acctgagtat tagagagaca aatggaaaaa tagttcctca tattttgcca
     4621 ttttttgagc taataccgag ggtcagctga ttgaacaccg tttatcgaga ggttgtgaaa
     4681 aagtcgctta gtttcgcgta ttaagaaaaa ttttgtgaaa tggctttcca catttttgta
     4741 taaatttggc ttaataccga gaaactggct tttgaatacc gtttattcgt aaaagggatt
     4801 gtgacaaaag tcttgtcaca atcccttttt atgtttttat tgttctaatt gcgctgtctt
     4861 gcttactgct tcttgcggtt tcacaaattt ttttgagatt ccccaacctg tgaaggcact
     4921 gaccatcgaa aacacaggag aaagcaaact gaaaaatacg aatggcaagt aagtgagtgt
     4981 aggaatacca aatgtacttg ctacgaaact tccggcaatt ccccaaggca ctaagtagtt
     5041 gattactgtt ccaccatctt ctagcgttct acctaataca gttggatcaa atcctgcttt
     5101 ttcataagtt tctttaaatg cattaccagg cagaataaca gataggaatt gttcaccaat
     5161 aaaaatattt actccgatac ttgtcatcaa tgttgtaaaa atcaaatttg gtgtgttcgt
     5221 catctttctt gatactcgtt ccatcacggt tgagatcaag ccaaattcga tcaacaatcc
     5281 acctaatgac aaagttagaa taatcaatga cacagtaggc atcatacttt cgattcctcc
     5341 gcggctaagc agtgaatcga tttgttggtt tccagtattt gaaacaaacc ctgattcgat
     5401 gaccccagcc aacttcgtta aggaagtttg tgggttttga ataaaaatca ttcctactgc
     5461 tacagcgata ttcaacaaaa tcgttaaaat tgccggcacc tttttccatg cacacagtaa
     5521 cattaaaaat aaaggaatca gtgaccagat tgaaatagta aagtttgctt ctagaacatc
     5581 agtcacttca cgaatagatg aaagacttgc ggctttactt gtgtgtccta aaatcatgaa
     5641 taaaaagaaa gaaacaacaa atgccgggac agttgaccac atcatatgct tgatgtgttt
     5701 gaacaaatcg gcatcaacaa tagctgctgc tagatttgtt gattcggata atggagacat
     5761 cttatctcca aaaacggcac ctgaaacaat agctccaaca accagcgctg gatttaagcc
     5821 tagtgttgtc ccaataccga aaaatgcaat cccaatcgtt gacataacag taaaggcact
     5881 ccctacagcg ctacctacga ttgcacacac aacaaataca gaagggacaa accactggac
     5941 actgatcaat ttgaacccaa tgaccattag agttggaatg atccctgctt gtatccatac
     6001 agcaatcaat gaaccgacta aaataaaaat aaagattggg atgattcctg gtttgatgcc
     6061 attgatgatt ccttcgtgca gccaatctgt cgggtatctt tttatcactg catacaacat
     6121 gaccattccg atcgctgcta agatcgtgat attaggtgag acctttaatc cgacaacgcc
     6181 aatcgcaatg cctaaaacaa taaccattaa tacaagtgtt gcttctaaaa aacttggtgt
     6241 tccttttttt cccatgataa tctctccttt tttagttgtt cttatgttct aaaaaagtta
     6301 agatatcgga ttactccgcc gcaattcatc cgcgattcct cctttataaa aataaaaaga
     6361 cctgttttaa actaaggacg aatgactccg cggtaccacc ttaattaaaa acagatctgt
     6421 ttttcacttt caaactttta ttcaaatgta tggactttcc atgtaattcg tcaattaggc
     6481 ttgatcggtt cacagcgacc accgactttc tgaacaaaac acctaaccgc tactgcgtga
     6541 aaagttgact tgtacgaagt ttaccttcca taagatcatt tgtcaatgaa aaagaaaata
     6601 ttttttcatt tttctgaaaa ttatttttat aactttcaca cacaagttta ttttttacta
     6661 gaaagtagcg ccctcttgtt tttttcttgc tactatttct tgatgttctt gcttgattct
     6721 ttctaatagc tttggctctt tcaacagatc aagagctgta ttggcgagta gttctgctgc
     6781 aatcgcaata gaagcaaggc ctttgtcact tttagcagca gctttgaatt cttctgtgtg
     6841 tccggcaata tatgtctcag agattgctac cgtaggctgg attgttggta tgacttggct
     6901 gacatttcct acatcagaag aaccgagact tgttctttgt tccgtttcta tttcttcatc
     6961 tggaacacct gcttcttttg catgctggaa gaataagtca tcaaaagaag gagtaacgat
     7021 gacatcatca actgcatttt ggaacaatcc aaactcaaaa gtcgtgcccg tacttatcgc
     7081 agcacctcgt acgatatttt ccactttttc atatacttta tctaatgttt gccgatttcc
     7141 tgcacgtaaa tagaaacgtc cagcagcgta atctggaatg acgttggcag ctactcctcc
     7201 gtctgtaatg atcccatgga tattgacatc ttttggcaat tgcaagcgta gagcgttgat
     7261 tccattaaac acttggatca atgcttccaa agcatttact cctttttcag gtgctgctgc
     7321 tgcatgggaa gcgacaccgt aaaatttgat gtcgactgga tcattcgcta aagattctgt
     7381 cgttttccca taacgaaaag ctggatggac gcataacgct acatcgacat cctcaaaaaa
     7441 accttctcga acaaagctgc ctttagctga tccgttctcg cccccttctt cccctggggt
     7501 gccatataca cggatttctc caccagtttc atcaatgaat tgcttcacta tgctggcggc
     7561 taatacggag taagtaccaa acaaattatg cccgcatgca tgaccaatac ctggtaaagc
     7621 atcgaattct gccaaaaagg caattgtggg accgggttta ttggaaatat aacgtgcatc
     7681 aaaggctgtt cggtgtccag ctacttcttt ttctacccaa aacctttctt tttctaattg
     7741 tttaatcaac gctgctgagg aataaaactc atagttactt acttcaggat gttcgtggat
     7801 atctagagca agtgcttgat attctggtaa acgatattgg attagatcct tcaattggtt
     7861 tgttgagatt gttgttgtca taaaaaattc ctcctatgct agtcattttt cttattttct
     7921 cccgaatgtt tcccatgcag gcacgcttga tccttttgat gttttctcga ttactttgac
     7981 tgtgtcttct gtttgataag cgtctactat tttttgatac gttttattgt tttcatcttc
     8041 ttttcttgca acgatgatat tcacataagg acgtgcatgg tcatccactg gttctaagaa
     8101 gatggcatca gaagcaggat taaatccagc atctacagct acaccgctat tgatcactga
     8161 aatgtctaca tctgacaaag cacgtgctgt ttgggaagca tctaattcag aaatgtctag
     8221 attttttcta ttctcggtaa tatcagaaac agtgggcgct tgtccttttg ccttatccac
     8281 tttgatcaat ccagcagttt gcaataacag aagagctctg ccgccatttg taacatcatt
     8341 agggattgct acttttgctc cgtctttgat atcagctaca tccttcaaag tgttagaata
     8401 aattcccagc ggtgcattga cagtatttcc gatcgatact aaatcagttc cgtgttcttt
     8461 attgtaattg tctaaaaaga attgatgttg gaaagaattc aaatcaatcg atccatctgc
     8521 taaagccaca tttggttgat tgtaatctgt aaatttcacg tattccaaat taatgttctc
     8581 ctttttcaaa cggtctttca cgttgtccca tacatctgta tcttctccaa taatccctag
     8641 ttttacgata gtttcttttt tgtcagttgt atctgctttt ccttggctac accctgccaa
     8701 ccaaataatt gctacaaatg taatgactac aaaacgaata atttttttca ttttttctcc
     8761 tccactttca atgtgtaatt tttttaatca gttgattact gataaattga cttaaaaaaa
     8821 ctaagattag aatcaataat gtagccacaa tcgttacatc tgtttggaat cgattatacc
     8881 ctctcgatat agcaagattc cctaatccgc ctgcacctac tgctccagcc atggtagtca
     8941 gtccgatcac gttgatgata gtcaacgccg agacccgaat gataccaggc aaaccttcta
     9001 acaaatacac acgtaaaatg ataccgattg gactggttcc catcgcttct gccgcttcga
     9061 tcaccccagg atcaacttct agcagtgcgt tctcgatttg acgagcaaaa aacggaacaa
     9121 ctccaaaaat caatggcact aaagctgctt tcgcaccaat cgtcgttcca actaagaaac
     9181 gagtagtcaa tgctaaaaga gaaagcaaga taatgaaagg gatggaacga atgatattga
     9241 tcagcttatc aagtatttca aatagcagac ggttagctag taatccatta gggcgtgtca
     9301 ctactaacgt cacacctaag atgattccaa tcatcccgcc aatgaatgct gtccaaaaag
     9361 acatatatag tgtttgcttc gtcgcttcga caaattcatc cggaatcaaa taaacattgg
     9421 gcaaatatgt tttaaagaaa tcagtcattt tatcttcctc cttcgatcac tttgatacga
     9481 ctatctaaat cgtttttttg taaaatattt atttttacac cagatttgat caaataatcc
     9541 aatgcttttt tccgctgttt ggtttctcca gctaaagtaa cgatcaaact ccctaacggc
     9601 gtttcttgta ataactctac attgccatat aaaatattcg ctgttacttg atatttgctg
     9661 tacagatgag cgattagagg ttcgttcgtc tgatttccaa tataacttaa ttccaccagc
     9721 catttattgg cgatctgatc ggcaaaagca gaatgggcaa tgatcgtttc taatgcttgg
     9781 tcaagatgag tagctgttcg aataaaatct ttggtcaatt cttcttcagg attgctgaag
     9841 atctggatgc tgctaccttt ttcaacgatt cttccatctt ccataaccgc taccttgttg
     9901 cagatttctt tgacaacttg catctcatga gtgatcagga cgattgttaa acctaactgg
     9961 cgattcaatt tttttaacaa tgcaaggatc tgaagggtcg ttttcggatc aagtgcactg
    10021 gttgcttcat cacataataa taccttaggt tcattcgcca aagctcgtgc gattgctact
    10081 cgttgttttt gtccccccga cagttgttta ggataagcag atgctttttc ttccaaaccg
    10141 acaagttcca gtagttcatt cactttttga cgacgttcct gtttcgattt tccagaatac
    10201 tttaaagaaa aatcaacgtt gtcaaaaata gttcggcttt ccattagatt gaaatgctga
    10261 aagatcatac cgatggcctt tctttctttt cgcaattctt tgggagagag ctgactcaga
    10321 ttcgtctgat tgacaatgac ttctcctgct gttggtttct gcagcaaatt catcacacga
    10381 actaacgtac ttttccctgc acctgagtaa ccgacgattc cataaacatc gcctttttca
    10441 atcgttaaat ctacagaatc tactgcctgt atctcttttt ctttttgttt gaaacataca
    10501 gaaacatctc gcaattcaat cattaaaatt cctccttatc taaaaaaagc gtcctcaaga
    10561 aaatgaaaat gtcattttct tgaggacgct tttttgcgtg ttaccacctc atgttcaagc
    10621 tgtgttcaca cacaactcct cttcaagtac gatcatactc tagcaagata tcggtcgcag
    10681 ccgtcatcgc cttacatcta tgctcgggat gaattgctcg atggccattt tccaatagtc
    10741 gttccttgct tcttctcagc taccgaagct ctctgtcaaa ggttcttcta tcgtacttac
    10801 catttcaatg caaataatat tcaaaaaaaa agtcctttta ctaacataaa ctatgtcagt
    10861 aaaaggacga cgtatcgcgg taccaccttt tttcggaagt ttccttccct caagcccaac
    10921 atctcaaaaa gagagattgg cgttcgtttt aacggtcgaa cagttccgaa tttgcctact
    10981 tatcgacaaa ttattctcca aggccatttt cagcttttct tacttgactc ttctcaccag
    11041 ccagagcctc tctgcaaagt ttccaaactt actttccttt tcaacgaaat cgatattttg
    11101 ttgttgggat taatctacat gaggaaaact gaaaagtcaa gtacgaaatt caaattattt
    11161 tttctatatt tatttttctg tttttttatt taacatttca ataacagttc gaagatcttt
    11221 tccttccgca agaagtttca atttttcctt ttcttcgacg gtataatttc ggacaaaact
    11281 ggaatcaggt atcaaaacaa cgcctaatcc tgctcgtgaa gcagctgttg ctcctactaa
    11341 agaatcttcc actgcaaagg ctgaactagc ttctaatcct gttcgttcca aagctgtcag
    11401 ataaggaatc ggtgaaggct tgttctccga cacttcgtct ccgaatgtaa acgtgtccaa
    11461 gtaatcagct aatttaaagt gatccaagat atctgttgct cttttcgtaa cagtggtcgt
    11521 agcaatgcct agtttcatat gacgttcttt taagtctgcc aatatttctt ttgcatatgg
    11581 ttttaagtgg atggctccgc ttgtcagctg ttgataaaaa tattcttcac gcttcgctcg
    11641 gacttcctgt actttttcag ggctacctgc tgctttcact aaaatatcaa aactttgttt
    11701 ccagtttttt ccgccccaag cgtcaacgat ttcttttggg atatggatat caaattgctg
    11761 aaatccccaa agccagccat cacgatacgt tttttctgtg tcaactaaca aaccatctaa
    11821 atcaaatatt gcacctttca tctatacatt aatcctttcc aaaacaatac acatagttta
    11881 gcacgactgg tccaaaaaga acagaagcaa agaaccagcc ttctcgttta taagagttct
    11941 tggaatcgac ggatatagat tttcttcaca gctgtcacaa gcgccagata agcgatgact
    12001 gtagcaaata accataacca gtaattgcca ggaagtggca tcaagcctaa ttcagcacca
    12061 aaacttgtaa aaggaaggac agaaccgaca ataattccta atgtagtgat aacaaacatg
    12121 gcaaatgttg cgttgctttg gataaatggt actttcggtg tacgcagtgc atggagcact
    12181 aaggtttgtg tccatagtga ttcgacaaac catcctgcat ggaataatgc gatgaaaatc
    12241 actcgctgtg aactatccaa tgtatggaaa cttccgccca ctacagcagg acaaataaca
    12301 aaatacatca acaagtaagt agtaatatca aatatcgaac ttgttggacc aaagtaaacc
    12361 atgaattccc caatactaga tgcattccat ttttttggct cgtccaaata ttctttatcc
    12421 atccgatccc atggcaagga aatgcaggaa acatcataga tcagattcaa aaacaaaagt
    12481 tgtaaaggca gcattggtaa aaatggcagg aacgtacttg caatcagcac ggaaaacata
    12541 ttgccaaaat tagaacttgc tgtagccttg atatatttca tgatattccc aaaagtttct
    12601 cgtccagaaa gaaggcctct ttccaaaatc gtcaaatctt tttctaaaag gatcacgtct
    12661 gcagattctt tcgcaatatc cacagctgta tctacagaaa taccgatatc agccactttc
    12721 attgcaggag cgtcattgat accatctcct aaaaatccga cagtatgacc atttttacgt
    12781 aaagctgtcg tcaaacgtgc tttttgctga ggattcaatt ttacgaatac ttcatggtct
    12841 tcagctactt gtgctaattc atgatcactc atttccgaga ttttctcacc ggtaaccaat
    12901 tcattgatct ctagacctac ttgacggcac actgaacgtg taactaattc gttgtcccca
    12961 gtcagaactt ttacagctac accatgttct ttcagagctt tcaacgcttg tttcgttgtt
    13021 tcttttggtg gatcaaggaa agcaagataa ccaatcaaaa ccatgtctga ctcatctttg
    13081 actgaaaatt ctccaacgac gcttggattg gtcttttgtg cgacaccaat cactcgtaag
    13141 ccatcttcat taagtgactg tacttttgca agtacggctt cttttcgttc ttttgttaat
    13201 ggtgtaacag taccgccaag atcaataaat ttagaaatgt tcagcatttc ttcaatcgcg
    13261 ccttttgtga tcatttgtgt tttaccagct ggatcagcaa ccacaacact catacgacga
    13321 cgttcaaaat caaaaggaat ttcatccact ttttgataat tgatcttatc tgtatctaaa
    13381 gtttctgttg cttcatcaat gatagccaca tctaacaaat ttttcagccc tgtttgatag
    13441 tagctattta gatacgcatg gcgcaaaaca cgatcatctt catttccttc acaatccata
    13501 tgatattcaa gaatgatttt atcttgtgtc aatgtccccg ttttatccgt acataacaca
    13561 tcgattgcac caaagttctg gatggcattc aaatttttga tcactgttcc ttttttggcc
    13621 atcacacttg ctcctttgac caaattcgtc gtaacgatca ttggaagcat ttcaggtgtt
    13681 agaccaactg ctacagatag tccaaaaaga aaagcttcta gccaatcccc ttttgtaagc
    13741 ccattgatga cgataactgc tggagccatc aatgacataa atttgatcag tagcatggat
    13801 gttttatgga tgcctaattc aaagcttgat tgaattggtt tttcagatag cgtttctgct
    13861 acatgaccaa atagtgtttc atttccaaca gaaacaatga ttccttccgc acttccactg
    13921 atgacattac ttcccataaa gacaagattt tcgtaacttg tttcgctttg ctgatcgtct
    13981 tcacatactt tcgcatgctt ttctactgga tagctttccc ctgtcaatgc tgcttgggaa
    14041 atgaataaat cctttgcgga caataaacgc atatccgctg gtatcatatc ccctgctgaa
    14101 agccgaacaa gatctccaca gacgatttct tccataggaa tctctgtgta ctttccatta
    14161 cgttttacag ctgccgttac tttcacaagt gatttcagtt tttcagccgc ttgattggaa
    14221 cgtacggact gaatcaacgt cattgtcccg ctgacgataa ccatagtgaa gataatggct
    14281 acaccgaaaa gatctttatc tccaggagct gctaatacat attccgtgat gaatgaaata
    14341 attcctaatg cgatcaacac taatgtaaat ggcgtgatgt atgctttcaa tatttctaca
    14401 atgaatggtg tttttttgcc atatgaaatc gcattttcac cgtaattttc tcttgaacgt
    14461 tcgatttgtt cttctgtcaa tccatttgct gatgcatgga atagttcaaa taattcttgt
    14521 ttcgtttttc cagcaaaagc tgcatagatt ttttgtgtat ctttcttttt ttccatctca
    14581 atctctcctt tgtgtccaaa agacagctgt tctcgctgct tttggtcttt agctattcat
    14641 cggatcgata gagaaaaaac tgaaaggggc tgagaacaga tagataatag ccctggcctt
    14701 tcctctagat ccttgttttt cgaatgactg tcatccattt gttctcatct cctttactaa
    14761 gtaattttaa ttcatgactt ccaatcattt ttcgaatagt tatttttcca attagaccac
    14821 ctccttttta tttttcaccg atggggattt gcggcaacaa aaaaacctct cgccaaatac
    14881 tcagcgaaag gtttgaaaaa gcatattaaa aaaagcttct cagatctcat cgttgagttt
    14941 tgacactgta caacgtaaaa agcagatacc tgcttttagc taccttatga aaagccttat
    15001 ctcgacaatt cctgttctac ccattggcgt ctctcgacgt ttttgggcag tagcctatgt
    15061 ttgtatagga gcctcaccta acaaatctta ttcggttttc cttcgttaat ataacaacta
    15121 aaaaagagga tgtcaaatca attttccatg tttttcatac tttttttaaa atacacacgt
    15181 tatattcctg tctcacgcgg ttttatttgt ctcattttcc acctcccgct ataatagaaa
    15241 tatagtagga aacgaggtgc tgttatggaa agaacacttg ttattatcaa gccagacggc
    15301 gtgagacgcc atttagtcgg aagcatcatc caacgttttg aagcaaaggg actggccatt
    15361 gctgaaatga agtttgaaac tatgactcct gaattagcaa aagaacatta tcagcacttg
    15421 actgagcgca gtttttttga tgaactgatc gattacatga cttccggtcc tgtcgtttat
    15481 cttgtgctgg tcggcgagga agtgatcgat atcgtccgta agatggttgg agcaacaaaa
    15541 gctgccgatg ctgttccagg tacgatccga ggagactacg cgctgccggg gactgaaaac
    15601 atcattcacg cttctgattc ccgtgatgct gcggtaaaag agatcgcccg ctttttcccg
    15661 ccattcgata taaacaaacc gtctgaaaaa gcggcgaagt gatacaaatt ctaaactgct
    15721 ggtctagttg ctgggttcag caatggccag cagttttttc tctaaggacc tccgttgatt
    15781 ctttcctaac atatattcga gcactgtgaa gaattcttgg tagtctttcg tcttttcttc
    15841 ccctaaagca gcagaaatca atctttgata ataatctgcc atcaatgttt catagatcac
    15901 tgtacttctt tctttatgtg ctaagctccg tttcatatct tcattcacac tacgtgcctt
    15961 tttcaaccga ccattttcta tataaaacga agtcaggttc attaaaaaag taaagagata
    16021 ctgctcatga ttgttcaact gtgctaatcg tcgcattctt tttttgattt tcaaataatt
    16081 gttctcaatg aaccgatcac tgaatatcca aggaaaatca gctagaatag atagttcaaa
    16141 ttgaccatag tctggtatct gcatcaagta ttcttcaatg attcggcatt cataacgtaa
    16201 aaattcaaat tctgtctcag atacttctgt tttttcgacc atgtgaagat tcaaaaccaa
    16261 ataatacaga tcttctgttt tcttaaaatg cctacggtta ttgctgatct ctttataagg
    16321 atcaaagctg tcttcttgta cccgatctag cattcgtctt tttttcttta actttattgg
    16381 ttcatccagc tggtttcgca tccattcata ttctgcaagt gtcatgttca ttcgctctaa
    16441 aaatgatctc aatgttacaa atgacatttt tttcactgaa tttgtctcaa tcccgactag
    16501 agaagtctta aaacagattc cttccgctaa ttgggactgt gtcaacttct tgttgactcg
    16561 aagtttcttg atcaattcac catcatacat aatagttctc ctttttaatt taaatttttc
    16621 cacattcaat aaatagatac gttaaaaagg aaaaatacac gaaaaaaata aaaaaatgtt
    16681 tttttcataa aaaataccaa ccttcaagca ttagaatgtt gtctaactac tgaaggttgg
    16741 tttgattgtt tattgattat ttttttgctg ttcaacgtct gttcttgtta ctggatataa
    16801 gttggttgtt gttttattac cattttttga aaagacactt gtcgaatgtt cgcctttttg
    16861 cttttcgatt gcttcattac gttccgcttc atttttatag tcatattttt tcggatcaat
    16921 cgtctcaaat ccgtttggca cataaaaccg caagagattt tcctgattca gcttatctga
    16981 aagacttaac gcagtcttga ctttttcctg atctttggag atttcctcat cgattcctgc
    17041 tgctttcgta tcgatgattt ctcctgtctg attattgtaa gcttttcctc ctaggacggt
    17101 gtatttgggt gtcacaaagt tcccatttcg aaaagcaact acttgatcat gctgagaaga
    17161 taataagtct gtaccaaaat gaagataatc tttatcatcg attcccaata gatgtagtaa
    17221 tgtcggcaaa acatcgattt ctccaccgta ttcatgattg actgtccctt tagtataacc
    17281 aggcatatgg atcatcaatg gcactcgttg catctgtgcg ttgtcaaatt catcccatgt
    17341 atctggatct ttaccaagag cgcttgctaa atctttatta tctgtattcg agatcccaaa
    17401 atgatccccg taaatcacaa agattgaatt ttggtaaata ccagattttt tcaaata
//
LOCUS       DACDXO010000057        17150 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E23605_2017
            SAMN12639989-rid9881463.denovo.058, whole genome shotgun sequence.
ACCESSION   DACDXO010000057 DACDXO010000000
VERSION     DACDXO010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639989
            Sequence Read Archive: SRR10023389
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17150)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17150)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741914.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:42:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,972
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 33 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 17 of 73
            Pseudo Genes (multiple problems)  :: 10 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17150
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639989-rid9881463.denovo.058"
                     /isolate="E23605_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            138..1475
                     /locus_tag="IWM35_002095"
     CDS             138..1475
                     /locus_tag="IWM35_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HAP9919938.1"
                     /translation="MSDIIIGILLVISFFFMVWYCVKGYNLMVGFAIMATVWMGLALV
                     GNTFSPNPAMEGQGVIDVLTHIYTTGPAEYAKSILVNVFFGAFFGRVLVDSGIAATLI
                     RKVVELGGDKPRITMSLLCIVTAVIFMSMTGIGPVISIAVIVLPILMSLGISVPVALF
                     SFMGSIMAGIFANIVNFKQYQTIYAGFNPAAESYTYNDYFQIGMIGMIVSLVVVLTVA
                     NISMNKKKRYAMAANVPAEGGDAPMISWLAVLLPVLGVVLLDLPIILGFILAGIWALL
                     FTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGFLMTLAMFNNSAAYASPYFSAIFGDW
                     IPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSAILAVVLAVNPTMSPAFLFPLFAITT
                     IAPQHLDITQSWVAWGLGYTKVTSREYMKKSIPTGWIIGAILCLITFLLYGNA"
     gene            1523..3652
                     /locus_tag="IWM35_002096"
     CDS             1523..3652
                     /locus_tag="IWM35_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HAP9919939.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLLENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKHLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKSIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPQTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            3627..4316
                     /locus_tag="IWM35_002097"
     CDS             3627..4316
                     /locus_tag="IWM35_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9919940.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKKIE
                     KRCTEYDLDYK"
     gene            complement(4724..5410)
                     /locus_tag="IWM35_002098"
                     /pseudo
     CDS             complement(4724..5410)
                     /locus_tag="IWM35_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
     gene            complement(5790..6230)
                     /locus_tag="IWM35_002099"
     CDS             complement(5790..6230)
                     /locus_tag="IWM35_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HAP9919941.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELDQLFQAYASLDDVNS"
     gene            complement(6234..7079)
                     /locus_tag="IWM35_002100"
     CDS             complement(6234..7079)
                     /locus_tag="IWM35_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HAP9919942.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVVWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLENQSKEQWLVIDGSALSLLAANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAIYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            7228..8646
                     /gene="pepV"
                     /locus_tag="IWM35_002101"
     CDS             7228..8646
                     /gene="pepV"
                     /locus_tag="IWM35_002101"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HAP9919943.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
     gene            complement(8703..9650)
                     /locus_tag="IWM35_002102"
     CDS             complement(8703..9650)
                     /locus_tag="IWM35_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP9919944.1"
                     /translation="MEQQEKIAVLIPCYNEEATISTVIADFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAVHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVRNTITRMYKTKILDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMFKDYKPLLFFGIWTIFFFLFGLVTGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYHSRK"
     gene            complement(9873..10223)
                     /locus_tag="IWM35_002103"
     CDS             complement(9873..10223)
                     /locus_tag="IWM35_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAP9919945.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKSSFLKEGAIEGSWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLEISRKKGATD"
     gene            10423..11502
                     /gene="pepA"
                     /locus_tag="IWM35_002104"
     CDS             10423..11502
                     /gene="pepA"
                     /locus_tag="IWM35_002104"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HAP9919946.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            11646..11966
                     /locus_tag="IWM35_002105"
     CDS             11646..11966
                     /locus_tag="IWM35_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HAP9919947.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VTKG"
     gene            11988..12452
                     /locus_tag="IWM35_002106"
     CDS             11988..12452
                     /locus_tag="IWM35_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAP9919948.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKAVEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKEMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYEIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            12657..13262
                     /locus_tag="IWM35_002107"
     CDS             12657..13262
                     /locus_tag="IWM35_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 domain-containing protein"
                     /protein_id="HAP9919949.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVDSYGMICSARELHLPNAPAKKGILVLPEDAVVGEAFPK"
     gene            complement(13301..14590)
                     /locus_tag="IWM35_002108"
     CDS             complement(13301..14590)
                     /locus_tag="IWM35_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HAP9919950.1"
                     /translation="MDRKNVTPKMKKSKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVLMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            15018..15497
                     /gene="rlmH"
                     /locus_tag="IWM35_002109"
     CDS             15018..15497
                     /gene="rlmH"
                     /locus_tag="IWM35_002109"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HAP9919951.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYALAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
     gene            15700..16250
                     /locus_tag="IWM35_002110"
                     /pseudo
     CDS             15700..16250
                     /locus_tag="IWM35_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299296.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
     gene            16321..>17150
                     /locus_tag="IWM35_002111"
     CDS             16321..>17150
                     /locus_tag="IWM35_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEfa13 family transposase"
                     /protein_id="HAP9919952.1"
                     /translation="MNDFTTEIMEILINKGDLDDLFRRHLELAINALLQAELTVFLDY
                     EKYDRAGFNSGNSRNGNYSRSFKTEYGELNLVIPRDRNGDFSQQTLPAYKRTNDSLET
                     TIIQLVQKGITMSEISDLIEKMYGHYYKPQTISNMSKIVSEDVSAFKERTLEAKYSVI
                     FMDATHIPLKRQTVSKEAVYIVIGIRLDGTKEVLGFTIAPTESAYVWKEILQDLKDRG
                     LEEVLLVVTDGLSGIDDSIHSVYPNAQFQQCCVHISRNIAHKVRVSDRQEICSDFKLV
                     "
BASE COUNT         5459 a         3105 c         3467 g         5119 t
ORIGIN      
        1 cataattaag gttttttaat tgaaaactga acatttatgg tcacccttta atgaaagcgc
       61 taactacata agttagaatg agtttgactg atatattagt ttgcttatca gaaaaacaaa
      121 atggacggag gaataacatg agtgatatta ttattggtat tttgttagtt atctcattct
      181 ttttcatggt ctggtactgt gtaaaaggct acaacttgat ggtcggcttt gccatcatgg
      241 ctactgtatg gatgggatta gcgctagtcg gaaacacgtt ttcaccaaac ccggcgatgg
      301 aagggcaagg agtaattgat gtgttgacgc atatctatac gacggggcca gctgagtacg
      361 ccaaatcgat cttggtaaat gtcttctttg gtgccttttt cgggcgtgtt ctagtagata
      421 gcggaatcgc agctaccctg attcgaaagg tagttgaact tggaggggac aagccaagaa
      481 tcacgatgag cttactatgt attgtaaccg ctgttatatt catgtcgatg acaggaatcg
      541 gaccagtgat ttctattgca gttattgtgt tgccaatctt gatgtcatta ggtatttcag
      601 taccggttgc actgttctca tttatgggct cgattatggc tggaatcttt gctaatatcg
      661 tcaatttcaa acaatatcaa acgatttatg ctggtttcaa tccagcggct gaaagctata
      721 cttataatga ctatttccaa attggtatga ttgggatgat tgtaagctta gttgtggtac
      781 taacggtcgc taatatatca atgaacaaaa agaaacgcta tgcaatggca gcaaacgtac
      841 cagctgaagg tggcgacgca ccaatgatct catggttagc cgtattatta ccagttttag
      901 gggtcgttct tttagatctt ccaatcatct taggttttat tctagcaggt atctgggcat
      961 tgctgtttac tggaaaattg cgcggaggtt ataaagaaat ctgtcgtcag tttgcgaaat
     1021 tatttacaga tggcgcagta gatgttgcgc caatggttgg tttcttgatg accttagcga
     1081 tgttcaataa ttcagctgcg tatgcttctc cttatttttc agctattttt ggagattgga
     1141 taccgcaatc accattagtc ttggcaatcg tatttgctat attgacacca ctaggatttt
     1201 tccgcggtcc aatgaatcta gtcggttcag gctcagctat tttagctgtc gtattagctg
     1261 ttaatccaac gatgtcgcca gcctttttgt tcccgttatt tgcaatcaca acgattgctc
     1321 ctcagcattt agatattaca caatcttggg ttgcttgggg attaggttat acaaaagtaa
     1381 cttctcgaga atatatgaag aaatccatcc caactggttg gatcatcgga gcgatcttat
     1441 gcctgatcac gttcttattg tacgggaacg cctagtaaac aaaacgtcga taaaaacgat
     1501 tagatgaatg gaggaaaaga aaatgaaacg ttttgtgcga atgggaattg atgttggcgg
     1561 tacacatacg aaagcagtcg caatcgataa tgcgacgcac gaaatcatcg gaaaatcttc
     1621 agtgaaaaca acccacgatg atgtccgtgg agtagccgca ggtgtcgttc agtcttttca
     1681 aaattgtttg ctggaaaata atatcagtcc agaagatgtt gtgtttgttg cacattctac
     1741 cacacaagcg actaatgcac tgattgaagg agacgtagca aaagtcggcg taatcgggat
     1801 ggcaaaaggt ggattagaag gttttttagc aaaacgacaa actagattga acgatatcga
     1861 tttagggaat aagaaaaaaa tagagattgt caatgcattc ttacctgtca aacacttaaa
     1921 tgtagaccgt gtttcagaaa cgatttcttc tttagaaaga gaaagagcgg aggtcttggt
     1981 ttcttctatg gcatttggag tagataatgg ggaaccagaa cgagttgttt atgaagcagc
     2041 atcggtaaaa tcgatcccta caacgatggc ttctgacatc acaaaattat atggattgac
     2101 aagaagaact cggacagcag caatcaatgc atctatttta ccaaaaatgc tagatacggc
     2161 aacctctaca gaagattccg tacgagaagc aggggtcaat gtttctttga tgattatgcg
     2221 cggagacggc ggagtaatgg aaatcaatga aatgaaaaaa cgacctgttt taaccatgct
     2281 ttccggtcca gcagcatcag tcatgggctc tttgatgtac ttgagagcgt ctaatggtgt
     2341 ttattttgaa gtaggcggaa caacaaccaa tatcggggtc attaaaaatg gacgtccagc
     2401 aatcgattat tcaattgtag gtggccaccc aacatatatc tcttcattag atgttcgggt
     2461 tctaggtgtg gcaggcggtt cgatggtacg ggctaatcag tcaggaatca tcgatgtcgg
     2521 tccacgttct gcacatattg ccggtctaga ttatgcagta tttacggaga cagaaaaaat
     2581 caaaggtcct aaggttgaat ttttctctcc taaagaagga gaccctgctg attacgtcaa
     2641 ggtagtgatg gaagacggag aagaagtgac tattacaaat acatgtgcag ccaatgtcct
     2701 tggattagtg caagaagaac atttttctta tggaaatgtc ccatctgcac gaaaagccat
     2761 ccaagcatta gctgattatt gtcatacgac ggttgaagac atcgcagaac agatcatgga
     2821 aaaatcctat gctaagattg aaccggtgat tttagaattg gcagacaagt atcacttgga
     2881 gaaagatcag atttctcttg taggtgtagg gggcggcgct gcctcattga ttacttactt
     2941 tagtaataaa atgggtgtga aatattcgat tcctgaaaac gccgaagtga tttcttctat
     3001 tggtgtagca cttgcaatgg tccgagatgt tgtagaacgt atcatccctt cgccaagtaa
     3061 agaagatata cgatcgttga aaaatgaagc gatgaataaa gcgattgaat caggcgctac
     3121 accagaatcg atcgaagtcc acgtagaaat cgatccacag acttcaaaag taacggctat
     3181 cgctactggt tctacggaag tgaaagcaac cgatctaacc aaggagatca caacagaaga
     3241 agcactagag cttgctgcag aggatatgcg attaaataaa aatgaagttt gcttattgga
     3301 aaatacacct ttcttttatg tatgcggcga acagaatcgt tcaaaaaatg ctggaagtct
     3361 tcggatcatc gatcaaaaag gatttatcaa ggtccaacgt ggacatgctt catgtatgaa
     3421 aacaactgca gccaattata tgacagctgt cgaacaactt tgggaagata tggcagttta
     3481 tcaaactgaa ttgattgccc gaccagaatt ttatctctgc ttaggagcaa gagtctctga
     3541 ttttacagcg acggatctgg aacaactaca gcttttgatg gatctggaag tatctactat
     3601 ggaaccggag gaagaagtga tcgtcgttgc cggaaatatc aaacaaacat aagttagaag
     3661 agttactaaa cgaattacaa cagatccccg aagaaatttg gggattctac cagtttcggc
     3721 gagatttatt ttggaaaaag attcctttgt ccaagcagaa aattcttata caacaatcaa
     3781 tcgattgtgg tatcgaaacg gcatgtagca tcaaaaaaaa gtaccctttt gccgatgtag
     3841 gagaaatttg tgaacaaatg gctgttccaa tcgtttcctg tgaatcagag caaataaacg
     3901 aacgaatcac ttttgcaact tatgcagaag atgaagggat tcgattgatg acggagccgt
     3961 tagaaaaatt aaaatgttca ggactagctt cgatctctaa agaaacagcg caagctttga
     4021 ttattggaca tgaactattt caccacatcg aagcttctgt taagggaatt tatacgcaaa
     4081 gcgaaaaaat tgtgctgtgg cggttgcctt tttatacaca tcaatcaaac attcgtgcat
     4141 tgagcgagat agcagcgatg agtttttcaa aagagatgaa tcaaagcgat ttttcgcctt
     4201 atgtattaga ggcagtctta ctttggccat ataatgaaat tcgtagccaa gggatcttag
     4261 aagaaataaa aaaaatagaa aaaagatgta cggagtatga tttagattac aaataaagag
     4321 actgtgacaa aagtcgtgtc acagtccttt ttttccgaat aaacggtgtt ctcctgtcaa
     4381 ctcctcggtc acaagtcaaa atattcaaca aacatgagaa gctgtttttt gaaattgttc
     4441 cttgtgactc ggagatgaac ggcaggctca cgacctcttg gaaaacggtg ttcaaaagct
     4501 tgcatctgcg gtaataagtc cagccaagtt aaaaacataa gaagctattt ttacttggct
     4561 gttcttatta cttgatgcag ggcagctttt tcacaacctc tcaataaacg gtgtgcaatc
     4621 agctgaccct cggtattagc tcaaaaaaaa cggcaaaata tgaggaacta ttttgccgtt
     4681 tctttctcta atactcaggt caaaacgctg attccaaaac ctcttatttg atgggtctgt
     4741 cctgaatatc ttcaaaataa tcggggtaat tatgaatcac gtattcttgc tcctcgataa
     4801 tcatatgtaa atgcatcgtg caaagaaatc cgacttcatc aaagttatga tcaatcattg
     4861 attgaagcat tgtctgatgt tcatctaata ctcgacccca gtctaattct gaaatcgtca
     4921 aacgtaacca tctgaaacgg ttcaagtgcg tactataact ttctagccaa tcccaaattt
     4981 ctcgttttcc agcaatttca taacaagttc ggtgaaaatc agtatccaaa tcaaaaaaag
     5041 cttttttatt ttgagcgatt actgcctgat cggtttgttc tagtattttt tctagtttag
     5101 cttgatctta cttggtcatt ttcgcactgc attcttgcag gatcggtttt tccaactttt
     5161 cccgcgtata acgggctaat gaagcagatc ggatatcgat ttttgaaaca taagtgccgg
     5221 actgtggaat ggtttttaca agctcttgat tgcgcagttg gatcagtgct tctcgaatcg
     5281 gtgtacgccc gatagctagt tgttcttcta acactttatc ggagatttta cttccaggtg
     5341 ctaactcgga ataaatgatt tgtttacgta tagattcata agcttgctgt tgcaaattat
     5401 tgataaccat ttaaccttcc tttctatgca gatataatct attataacat aaatcgctta
     5461 caaatcgtgt gggcataaaa acacagtctg caaaaaaagc cctgataggc atgcctatta
     5521 gagcctcgaa aagaaaaata ttgaatcaat ctggtcgtac ctcttggatc ggtttgacac
     5581 gaccgccgat tcttttagca aaagcttcgg attctttttt gtctttgaaa atgagcagat
     5641 tgtctaagtt tttttcccga gtgcgacagc cagttttatc gatgtaaccg tgcattactt
     5701 taacgacata catggggatc acctgctttt ttcttttcat tatacgaact actcgacaca
     5761 atacaagaaa aatgcttaga cggtctttat taagaattta catcatccaa agaagcgtac
     5821 gcttggaata actgatctaa ctccggattg actttgctca gcttcactaa tcgttgcgaa
     5881 tcagaaagta aaaaacagtg tttactactg ccagtagttt ttaatttttt gtcttcagtt
     5941 cccacaattt cgtagctaaa atccaaccgt gtccccgtat acttttggat agttggatgg
     6001 atgctcactt tttctccgta atggatcatt tctttatagc tgcaatttac ttccagaacg
     6061 gggatgatga tcccagattc ttcgattttt ttatagggaa accctagatg taccaataag
     6121 gctacccgag cctcttcaaa ccagcggata taattgctgt gatgaataat ccccatttga
     6181 tctgtttcat aataaaaagg ttctcgcaca taacctgtaa atttctccat gactcatgcc
     6241 tcgttttcca ttgatttttt acaaaaataa ttcatccatt tcggaagtgc ttcgctgatt
     6301 tgtgtcggca aaacaatata ttgttctttt gccagctggt ctccaatgaa actatgaaga
     6361 taaatagctg ctgcaatcgt ttggtcattt tgaggaaact gggctaaaaa tcctgtgatc
     6421 atcccagcta aagtgtcacc catgccgccc gttgccatag cagcagaacc taaaggattg
     6481 gtgtagaagt gctggtgacc ataaattttt gtctcatgac ttttcaaaac gagcacagca
     6541 ccgatttcct gttgcttctt tttgttttct ttatcccgtt gttcttgtat cggtagagaa
     6601 ctcaaccgtt gccactccat ttgatgaggt gtaagtacag tttgttcggg atagggtagc
     6661 aaaagatgat tagcagcaag taaacttaaa gcagagccat caatcaccag ccattgttct
     6721 tttgattggt tttctagtac ttgtttgaaa cgttccaaac tcttagaaga agtacccaat
     6781 ccgggaccaa tcaagagtac atctgcttgc tttattacgg aaagaaactg gtcagtatcc
     6841 gtccaaacta caatcattgc ttctggcaga tgactgtgaa gagcactccg atttttttca
     6901 tcagttacta cagtcgtgag acccgctccg ctgtgtacgg cagctaaagc actcataatg
     6961 attgctccgc catattgttc attgccgcca atcaaaacga tacgtccaaa tgttccttta
     7021 tgtgaaattt ttggacgagg tgtgatgact cttttcacta gttgttcgtt tatcagcatc
     7081 taaaaacctc cttttactca ttatagcgta tatttttttt agagaaaaac agataagaaa
     7141 aggaattgtc agattttttg aactattttt ttctgtaatt tgtggtatca tgaacacgaa
     7201 gacttaaata agtggaggat taactttatg acaattgatt ggcaaaaaga agtagaagca
     7261 cgtaaagaag acttgcttga agacttgaaa aatcttttac gtgtcaacag cgagcgcgat
     7321 gactcaaaag taacaccaga tgcaccattt ggacctggac ctcgcgacgc attgaaacat
     7381 atgttagctt atggcgaacg tgatggattt aaagtaaaaa atgtcgataa ctatgcaggg
     7441 catattgatc ttggcgaagg agacgaaacg ctagggatat ttggacacat ggatgtcgtt
     7501 cctgcaggag atggctggga cacagatcct tatgaaccag taatcaagga tggaaagatc
     7561 tttgcacgcg gatcaagcga tgacaaaggt ccaagtatgg cagcttacta tgcgatgaag
     7621 atcattaaag aacttgactt gaaactatca aaaaaagttc gcttcgtagt cggcagcgat
     7681 gaagaaagcg gctggggcga tatggcttat tatttcgaac atgaagaaga accagatttt
     7741 ggtttctcac cagatgctga attcccaatc atcaatggtg aaaaaggaaa tgtttcttta
     7801 gctttacgct tcaaaggcga caatgcagga gattatgtct tgaaatcttt tgtttccgga
     7861 cttcgcgaaa atatggtgcc aggaacagca actgcagcac ttcaagtacc aagtgcagat
     7921 gcagctatcg cgatggaaga agcattttac caattcatcg aagcaaatcc agtaagcgga
     7981 acgattgaag cggacaatac gtatgtaaaa atcgaattag ttggtaaagg cgcacatggt
     8041 gcaagcccac aatctggtat caatgcaggt tctttcttgg cattgttctt agataactat
     8101 gaattcctag gatctgctaa acaatttatc catgtagcag ctgcttatgt ccatgaagat
     8161 ttttatggag aaaaactagg agtcgcttat gaagatgaaa aaatgggcaa attgacaatg
     8221 aatgctggac tatttgcttt tgaagaaaat ggtacagagg aagcgaactt catcaatatg
     8281 aatttccgtt ttccaaaagg tgtaacagta gacggattgc aaagtgacat cgaacaaaca
     8341 gtggggcaag aaggcgcaac agttactcga ggtgctcgcg taatggaacc acattacgta
     8401 ccaatggatg atccattggt ggctacatta ttgcaagtct atgaagacca cactggagaa
     8461 aaaggctacg aacaaatcat cggtggcgga acttacggac gtttgttgaa acgtggcgtt
     8521 gcttatggag cgatgttccc aggatatact gatacaatgc atcaagcaaa cgaatttatg
     8581 agtcttgatg acttgttcag agcaacagca atctatgcag atgccatcta tcgtttggcg
     8641 aaataattaa ttaaaaagag gatcaagaat tgcaaattat ttttgcagtc ttgatccttt
     8701 ttttactttc tactgtggta ttcatgataa acatgatata gattcaattc atattctttc
     8761 ttagcgttcg tgacgactgt atccaaaatc aaccctgtta ctaatgaaag aagtgctaaa
     8821 gtcatcaatc cagtacttaa aatcgcagaa gggatgcgtg taataaagct tgtcagcata
     8881 tattcgcgaa tcacaggaat ccccgtgacc aatccaaata aaaagaagaa gatggtccaa
     8941 atcccaaaga aaagcaaagg cttataatct ttaaacattt taataatcat catgatcact
     9001 ttgaatccgt cagagaatgt attcaatttt gattcgctgc cttcaggacg atcacgataa
     9061 tcgattggga tttcaacgaa cttgaatttt ttatctaaag catggatcgt caattccgtt
     9121 tcgatttgaa aaccagaact cataatagga aaacttttga caaagatacg attgaatcct
     9181 cgatagccgg tcatgacatc aagaattttt gttttataca ttctagtaat tgtatttcta
     9241 acaaggttat ttccaaaatc gtggaaaggt ctcttattct catcaaagta ggttccattt
     9301 gataggcgat caccaatgac catatctgct tcaccagaac gtaatttctc gagcaaacca
     9361 tgtacagctt ctgcaggata agtatcatcg ccatctacca ttaaataata gtcggcatcg
     9421 atatcaaaga acatttggcg gatcacattt ccttttcctt gacgaggttc tttttttacg
     9481 attgctccac cttgaacggc caattcgtat gttcgatcgg tcgaattatt atcatagaca
     9541 taaatatctg cttctggtaa ttctcgttta aagtctgcaa tgactgttga aattgttgct
     9601 tcctcattgt aacaaggaat cagaacagca attttttctt gttgttccat ttttcactct
     9661 ccttaactat agaaaacaaa agctaaatta aaaatacctc ataatcaaag gaaatgctag
     9721 tttttcccgc taaatatcat ttatttaaaa agaaaatact ggaatatatg tttggaaagc
     9781 tctatctaaa aaaccaccca ctacaaaaga aggacatgat acctctcttt tgaatagtgg
     9841 gtgcgtattc gttctctcac ttttttgacg tattaatctg ttgcaccttt ttttcttgaa
     9901 atttctaata cggttcctgt atgagcatct gctaaaaatt cataataaac agtttcgcca
     9961 tcttctattc gagaaagacc tccccgataa gcactcgacc gaacggcgaa tttcctaata
    10021 ggttcctttt caaaagagat ccaagaacct tcgattgcac cttcttttag aaaagatgac
    10081 ttgatatttt caagtacttg gtctgctgac aatacttttt tgcgatgata ccaaatcgtt
    10141 gaagctaccc cgctcaataa tcctagaccg attccaactg ctaacccgcc tttaaaatag
    10201 cttagttcgc tttcttcata catgtggaca tacctccttc ttctcacttg ttactccttg
    10261 tgcttatttt accacactct catattttct ttgaaataaa taaccgctac cagaaaaaat
    10321 aagctataat aaatacgatc ggtctgcaga aaaaatcata ttttatccgc agatcaatat
    10381 aagtctgaga ggagtttagt ctgaaattta gacgaatagc atatggaaga aaaaacattt
    10441 caacgaatca aagagttaac agaattacaa ggaacaagcg gttttgaaca agatgttcgc
    10501 gcttatatgg aaagtcatat ggaaccgctg gtagatgagt tacagttaga tggtttgggc
    10561 ggcatttttg gtctgcgcca tcatgaagaa gcggatgcac cccgtgtaat ggtcgcagca
    10621 catatggatg aagtcggatt catgctgaca caaatccaag aaaacgggct ttttaaagtc
    10681 gtacctctag gcggctggaa tccttacgtt gtttctgctc agcgttttac acttaaaacc
    10741 tctactggta agaattatcc atgtatttct tcttccgttc ccccacattt attgcgagga
    10801 acaagcggac aaaaatcagt agaagtaacc gatattttgt tcgatgctgg gtttgaatca
    10861 agagaagagg caatgagtta cggtgtcctt ccaggagaca caatcgttcc ttacgctgaa
    10921 acgatcaaaa cagccaacgg aaagaatatt atcagtaaat catgggacaa ccgctatggc
    10981 tgtacaatgg tattagaagc tttagaagcg ttgcaaaatg aaacattagg tcatacattg
    11041 attgcaggcg cgaatgtcca ggaagaagta ggcttgcgtg gttctaaagc ttctgtcaat
    11101 aaattcaagc cagatctgtt ctttgcagta gactgctctg cagcagatga tacagtgacg
    11161 aaaaatggca cattcggaca tttaggagaa ggtacattga tgcgtatcca agatccaggt
    11221 ttgatcatgc ttcctcgttt gcgcgagtac ttgctggata tcgctgaaac aaacaatatc
    11281 ccttatcaat actttgtttc aaaaggcggt acagatgcag gtgctgctca tacacaaaac
    11341 gaaggaatcc caagtacagt gattggcgta gtgggacgtt atatccatac gcatcaaacg
    11401 atgttcagta ttcgcgattt tgaagctgca cgagaaatgt tgattcaaac actgaaaggt
    11461 ttggataaat caacagtaaa tacgatcgta tacggaaaat aatgcgatag tgaaactata
    11521 aaatctggca aaagattgct ttttgccaga ttttttttat aaaaagagga tcaatgtttg
    11581 ttagttcgaa agaaaggtgt ataatttaat tgaaaaatac ataaacagaa agtagaggac
    11641 aaagaatgat tatcccaaat agtttagaag aattagcagg ctacgtagaa actggaaaga
    11701 gtgtattttt ctttactgcc ggctggtgcg gagactgccg ttttatcaaa ccgcaaatgc
    11761 ctgaaattga aaatgacttt tctgattggc aatttattga agtagaccgt gacaagtaca
    11821 tagatgtagc cgctgaatgg aacatttttg gtatcccaag ttttgtcgtg atccaagacg
    11881 gaaaagaatt aggtcgtcta gtgaataaag atcgtaaaac aaaacaggaa atcgaatcgt
    11941 tcctagaaag tgtaacaaaa ggctgacatc tgttaggagg ggtcaagatg aatcaagaat
    12001 ataagaatat tttagtaggt atcgacggta gtgaacaagc acttgctgct ttcaaaaaag
    12061 cggtagaagt ggctcgccgt aataatggga aagtatatgt agcaaacgta atcgatcagc
    12121 aattttataa ctttatgggc tatgcaccta ttgatcaaaa tctagtcgat cagcaaactg
    12181 aaacagccaa ggaaatgatc gaagaatgta aagaatatgg gaaatctgtt gattacacaa
    12241 atatagaagg aattattgca tatggttctg ttcgtgaatc catggcagtc aaattgccaa
    12301 aaaaatatga gatcgatcta atcatggttg ggcagtcagg tctaaatgca gtcgaacgct
    12361 ttatgacagg aagtgtagcc agctacgtga tacgtcattc accttgtgat gtcttgatcg
    12421 tttctgacga agaagaacag ccaagtaatt aagcaagtgg aacttgtacg acctttttaa
    12481 tcaattaact aaaacagtaa agggactgtg acaaaagtct tgtcacagtc ccttgttacg
    12541 aataaatggt gttccagaag aggcttcttc ggaaataaag catgaaaaaa atttcatgca
    12601 gtgaatgcaa ttttttgtta agataaatct cagcaaagga taatggagga aagaacatga
    12661 tttttgctta taacaaagca cacgtcggag acaccttgat ggttattata gccgacgata
    12721 aaggaacaga aaacactgtt gaacgcaaat ggaatgttgc tcggatcaaa gatgaacaag
    12781 gtcaagttgt tgcttggaac tttttccata tctccgatca tctaacaatc gaagggaacg
    12841 gccaagtaag cttaacagat gaggaattag cgaaattgaa tcgattgatc aaagaagcag
    12901 gattcgaaga acagcttgaa gctgatcatg aaccgaagat cgttgtcggg tacgtaaaaa
    12961 catgcgtccc acatcctgat tctgaccatt tatcgattac tgagacagaa gtagacaatg
    13021 gtacagtatt gcaaatcgtt tgcggtgcac ccaatatcga agctggacaa aaagttgtcg
    13081 ttgcaaaacc aggagctatg atgccagatg gaatgatgat ctggccagga actttgcgag
    13141 gggtagatag ctatggaatg atctgttccg ctcgtgaatt gcatttgcca aatgctccag
    13201 caaaaaaagg aatcttagta ttgcctgagg atgccgtagt cggagaagca tttccaaaat
    13261 aaagaggttg tgacaagtct ttgtcacaac ctctttttta ttaattttca ctattttgat
    13321 tttgttttaa tgccgattga tcgatcgtca attcaacagt agttgttttt tctttcgagc
    13381 cttcatagta agtgattttg acagaatctc ccactttttt cttatacaaa gcagattgaa
    13441 gttctacacc agaagaaact tctttgtcat cgatcttagt aatgacgtcg tattgtttca
    13501 atccagcttt ttcagccggt gtagctgttt ggacatttgt cacgatgaca ccgttagtta
    13561 cagatgaagg aatcttcaag atttgttctt gttgctgagt agaaacggca gataagtcga
    13621 ccattgtgat acctagagct ggacgtgtca cttttccgtc tttttccagt tgattgatga
    13681 tattcactac atcgttactt ggaatagcaa atcccattcc ttctacgctt acattagaag
    13741 tagattcaga agtacttgcg atcttacttg agttgatacc gataacttgt ccttcgatat
    13801 taactaaagg accaccagaa tttccagggt tgattgctgc atctgtttgg atcgcattga
    13861 tattgactgt ttcgttcgat tcattcgtac ttgtcacttg tcggttcaag gaagaaatga
    13921 ttcctgaagt gacagaattt gcatattcag atcctagtgg ggaaccaatc gcaatcgcag
    13981 gctcaccaac cttcagagca cttgagtcac caaaagaggc aactgtttcg actttgtctg
    14041 aattgatctt caacaccgct aaatctgaat aagcatctgt tccaactaat tcagctttta
    14101 ccttcgtacc gtctttcatc aagacttcaa gaccttgctg gccatctact acgtgattat
    14161 tcgtgactac ataagcagta ttcccgtctt ttttgtagat cacgccgctt ccttcactat
    14221 aagcttccaa attactatca tctgagctgc tactttcttc ttgctgcccg aatagtccgc
    14281 caaaaccgct tgattggttt tgactttgta agttgataac agaaacgaca gcgtcttgaa
    14341 ctttatctac cgctttggtg atatcagaat ctacattgac tttgacattt tccacaactg
    14401 tttcaccggc agaattctga ttgccgcttg tagcagtaga actattgttg ccagtgccca
    14461 tcgctgcata aaaaatgcca gctgtcagta gtcctccaac gatccctcca acaagcccca
    14521 gacctaactt acgccagagg ctattatttt tacttttttt catctttggt gtcacatttt
    14581 ttctatccat gtgttcctcc tccaattaca ttcatttatc tactgttagt atagtcttat
    14641 ttgttgactt tagtctactt tgagactgtg aactttttat gaaaaacttt aggaaaaatc
    14701 attgttttca gatgaaaata tttgattttc tttttccaca gatactgtgt aaaactacgc
    14761 ggataggatt ggaaaaataa gggataaaac atatccacag cctgtgtata aagtggataa
    14821 attcttataa aacgtatgtt tgcctaatga atgtgatgaa ataaggtaaa ataacactgt
    14881 tttctgtgga taaacctgtg gatgttgtgc ataactgttg gggaaatctt tttaacaaaa
    14941 atagccaaaa taaacatata gacgaaaaaa ttgattataa taaacaaagt gagcatgaag
    15001 accaaaggag agaaaacatg aatatcaaaa ttatctcagt aggaaaatta aaagaaaaat
    15061 acctgatcca aggtatcaat gagtatgtca aacggctgaa tgcctatgca aaaatcgaat
    15121 tgatcgaagt tcctgacgaa aaagccccgg agaatttaag cgaagcgcaa atgagacaag
    15181 taaaagaaaa agaaggggaa cggatcttag cgaaaatcaa agaacaagaa tacgtctatg
    15241 ccttagcaat tgaagggaaa aatcctacaa gtgaatcttt tgctaaacaa atcgaccaat
    15301 taggtatcca aggaaaaagc caccttgttt ttgttatcgg cggctcttta ggactcagcg
    15361 aagccgtcat gaaaagaagc aatgctcaga tctcttttgg aaaaatgacg tatccacatc
    15421 aattgatgcg tctgatcctt gtcgagcaaa tctaccgtgc cttccggatc aatgcggggg
    15481 ctccatacca caaataatga tattttttaa gagaaaaaat tatccctatt ttaaataata
    15541 gcaataggta gtggtaggga gaaatttata aataactttt ttatttgctt tggtagccaa
    15601 gtaagttgca aaagaaaaat agatactgtt aaaaagataa tgtagacata gaaacaaata
    15661 cgtaaaattt tagttagaat aatggcaagg agaaaaaaca tgatagatga aaaaaagtgt
    15721 ttaaaaaatc tattagagga tatggatgat attgaagaaa actatctaaa tggactggga
    15781 aaacaaatat atttcagata cttaagctat ctaaaaatga aataagacat agtaattttt
    15841 tagctttttt gtttgctcca aatgaaaccc acaatttatc tgatgagttt tttaaaatgt
    15901 ttttgaaaag gtatatagat tctaatgatg ataccaaagc tgctatcaat tattttgatg
    15961 ctttacttaa tagctatgaa gatttaattg tttacagaga aaataagaat attgatattt
    16021 tattggtatc ggaaaaaaat aaaatagtgg tgtgcatcga aaataaaatt ctgtcttcgg
    16081 agtctggagg gcaacttaat aaataccaaa tatatataga acgcaattat tctaactata
    16141 aaaaaatttt tgttttttta actccagatg gaaatgaacc cacaaatcct agttggggca
    16201 taattactta caagaatatc gttgaagaga gtgtaaaata ttttgtgtaa atagaaaaaa
    16261 ggaagtccct tctgtagaat agagttacca caacacattc acagaaaaga ggacttccat
    16321 atgaacgatt ttactacaga aattatggaa atactaatca ataaaggtga tttggatgat
    16381 ttgtttcgtc gtcacctaga attggctatt aacgctttat tacaagctga attaacggta
    16441 tttcttgact atgaaaagta tgatagagct ggatttaatt caggtaattc ccgtaatggg
    16501 aattattcgc gctcttttaa aacagagtac ggtgaattga atttagtaat acctagagat
    16561 aggaatggag acttttctca acaaacttta cctgcttata aaagaaccaa tgactcttta
    16621 gaaaccacta ttattcagct agttcaaaaa ggaattacta tgtccgaaat ctctgatcta
    16681 atcgaaaaaa tgtatggtca ttattacaaa ccacaaacta tttcaaatat gagtaaaatt
    16741 gtgtctgaag atgtttcggc atttaaagaa agaactttag aagctaaata ctcagtcatt
    16801 tttatggatg ctactcatat tcccttaaaa agacaaaccg tatcaaaaga agctgtctat
    16861 atagtaattg gtatcaggtt ggatggaact aaagaggttc taggatttac tatagctcca
    16921 actgaatctg cttatgtttg gaaagagatt cttcaagatc taaaagaccg tggtttagaa
    16981 gaggttttgt tagtcgtaac agatggttta agtggtattg acgatagtat tcatagtgtt
    17041 tatccaaatg ctcaatttca acaatgttgt gtccatatat ctagaaatat cgctcataag
    17101 gttcgtgtta gtgatcgaca agaaatctgt agcgatttca aattagttta
//
LOCUS       DACDXO010000058        17021 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E23605_2017
            SAMN12639989-rid9881463.denovo.059, whole genome shotgun sequence.
ACCESSION   DACDXO010000058 DACDXO010000000
VERSION     DACDXO010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639989
            Sequence Read Archive: SRR10023389
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17021)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17021)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741914.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:42:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,972
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 33 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 17 of 73
            Pseudo Genes (multiple problems)  :: 10 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17021
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639989-rid9881463.denovo.059"
                     /isolate="E23605_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            226..894
                     /locus_tag="IWM35_002112"
     CDS             226..894
                     /locus_tag="IWM35_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HAP9919953.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFSLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
     gene            complement(1094..1480)
                     /locus_tag="IWM35_002113"
     CDS             complement(1094..1480)
                     /locus_tag="IWM35_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9919954.1"
                     /translation="MRRTAKKFIDYNEYRDRPFGLKWGTAFAMEELVNGIKKNEEEAR
                     KQPLPKQQMTLQEIDSILTEAFLSRKAVIIQLNLKDEFGRLLDEVEGHFIGEAYEDYF
                     MIDHQPILWEDVRYIELKETKKWFVT"
     gene            complement(1452..2834)
                     /locus_tag="IWM35_002114"
     CDS             complement(1452..2834)
                     /locus_tag="IWM35_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HAP9919955.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            complement(2990..4177)
                     /locus_tag="IWM35_002115"
     CDS             complement(2990..4177)
                     /locus_tag="IWM35_002115"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HAP9919956.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            complement(4184..5215)
                     /locus_tag="IWM35_002116"
     CDS             complement(4184..5215)
                     /locus_tag="IWM35_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAP9919957.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGRFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVGIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            complement(5231..6685)
                     /locus_tag="IWM35_002117"
     CDS             complement(5231..6685)
                     /locus_tag="IWM35_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP9919958.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANVATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEELENKLAPADTMEDELHDAVVPSH"
     gene            6860..7765
                     /locus_tag="IWM35_002118"
     CDS             6860..7765
                     /locus_tag="IWM35_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP9919959.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     ENFKNIALDTLKKQLEKQTAVSWTT"
     gene            7856..8533
                     /locus_tag="IWM35_002119"
     CDS             7856..8533
                     /locus_tag="IWM35_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAP9919960.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            8814..9710
                     /locus_tag="IWM35_002120"
     CDS             8814..9710
                     /locus_tag="IWM35_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP9919961.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(9765..11135)
                     /locus_tag="IWM35_002121"
     CDS             complement(9765..11135)
                     /locus_tag="IWM35_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP9919962.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            11373..12119
                     /locus_tag="IWM35_002122"
     CDS             11373..12119
                     /locus_tag="IWM35_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAP9919963.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQPVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            12116..12955
                     /locus_tag="IWM35_002123"
     CDS             12116..12955
                     /locus_tag="IWM35_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAP9919964.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            12956..13906
                     /locus_tag="IWM35_002124"
     CDS             12956..13906
                     /locus_tag="IWM35_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HAP9919965.1"
                     /translation="MKTKKSLFLILAVSFLVLAGCGKQASDQADEGSKEKLSVVATNS
                     ILADMAKEVGTDQIDIHSIVPVGTDPHEYEVLPEDIKKASDADVILYNGLNLETGNSW
                     FDNLMETAKKEEGKDYFAVSKNVEPLYLTSGEEHTKADPHAWLDLSNGIKYVEEIARI
                     FSEKDAENATLYKKNAEAYVEKLKELDTQAKESFASIEENKKLLVTSEGAFKYFSRAY
                     DLPAAYIWEINTESQGTPDQMKAIIDQIRAKEVPVLFVETSVDSRSMERVAKETGLKI
                     YDKLFTDSIAKEGEQGDSYYQMMKWNIETIHEGLSQTKES"
     gene            14047..14799
                     /locus_tag="IWM35_002125"
     CDS             14047..14799
                     /locus_tag="IWM35_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9919966.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            14818..15969
                     /gene="queG"
                     /locus_tag="IWM35_002126"
     CDS             14818..15969
                     /gene="queG"
                     /locus_tag="IWM35_002126"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HAP9919967.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEVYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRKLK"
     gene            16353..16961
                     /locus_tag="IWM35_002127"
     CDS             16353..16961
                     /locus_tag="IWM35_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP9919968.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
BASE COUNT         5560 a         3172 c         3391 g         4898 t
ORIGIN      
        1 cgtccaattt cgtaaaacaa ccgtaacggt tgtgtaaaaa tatcatttta gagcataaaa
       61 aaaaggagtg gatttctcca ctccaaaaaa gtctaacttt tgggggtcac tacagtgtca
      121 cagtcctttt tttagtaaaa ggatacattt cttccttgta tattccatgc gtgttcgtga
      181 gaaagcatat ataatttaat taacagatag ttagggggag aaaaaatgat tttttcaatt
      241 acaatgaaac tggtgttcgg gttgatcggt ttattattag tggtacgttt gcttgggaag
      301 aaatccatgt cagaaatcac accgtttgat ttagtctata cgcttgtttt aggtgggatc
      361 ttagaagaat caacctatga tgacaatgtc catgtagggc atgtcctatt tgccattgcg
      421 ctttgggcag taatgattta tggtatcgaa cgtatcgtcc aaaaaaatga aaaggtcaat
      481 cgttgggtca aaggcgagcc atctgttcta atcaaagacg gcgtcatcaa catgacagag
      541 ttgacaaata accatatcga gatggagcag ttgcgagcaa tcttaagaca gcaagaatgc
      601 ttttctcttg aaaatgccaa acatgttatc ctagagaatg ctggtcaaat gagtgtattg
      661 aaaaaatcag atgaagacaa agctttatcg atcctgcttg tagatgaagg gcaaattcag
      721 cataaagtgc ttcagtccaa tcaattgact gaagcatggc ttatggagaa cttgaagaaa
      781 gaaggctatg cagatgtaaa acagctgatt tatgttgaat ggtcagaaga aaaagggctc
      841 tacatactaa ataaagaaga cgtcatgcat gaatcttacc gaatagatgg atagatagac
      901 agaggttgtg aaatcagcgc cttggcttgt tactcaaaac taagcgactt tttcacaacc
      961 tctgatatct aatatacgtt gttccagaag ttgcttcttc gaaaaataag ccaaaattcc
     1021 acaaaaattt aagaagcaat tttcgaaaaa tcctttctta ttttttgaag ttgatcactt
     1081 ctgtcacaac ctctcatgtc acaaaccatt tttttgtttc ctttaactca atataccgta
     1141 cgtcttccca caatatcggc tgatggtcaa tcataaagta atcttcatag gcttcaccaa
     1201 tgaagtgccc ttcgacctca tccaagagtc ttccaaattc atcttttaaa ttgagctgaa
     1261 tgattactgc tttacgagat aaaaaggctt ctgtcagtat actatcgatt tcttgcaatg
     1321 tcatttgttg tttaggaaga ggctgtttgc gtgcttcttc ttcatttttt ttgataccgt
     1381 tgactagttc ttccatcgca aaagcagtcc cccattttaa tccaaatggc cgatctcgat
     1441 actcgttata gtcaatgaat ttttttgctg tccgtctcat cttttccacc ttctaatcca
     1501 ctcataccac ctgcatgccc gccaacgaga gaactacgtg cgatggcgcg tcctccttgc
     1561 aagagactat gcgcatggat gattgattga aagccgtatt tttttctaat cgtatcgaca
     1621 acaaaatcaa ctttcgcttc atttatctgc ttttcaggat cttcaaaaag gttaagctgt
     1681 aaacctgtcg cgtatacgag gtcggaacag ttcacgccta cacgccgaat atcctggtag
     1741 tggtaatgct ggtggaaaat ggtcaataaa gcatctgcta gatctctgct agcatttgtc
     1801 gcttcgattt tcatttgctg gtgaaacccg gtcttgccat ttggatcaag atagcctaaa
     1861 gagtatccta cccataagct gactacttgt gtttttgctg cagatcgtct caagcgagtg
     1921 ccgacttggt cggccatttc tttgataacg atctcgattt cttctcgtct tgtataatct
     1981 ctatctagga tttgactatt tccgatactt tttgattttg gtgtatattc ttgtcccaaa
     2041 aaacttcgat cgatgcccca agcatgggca taaagctgag taccgatgac ccctagcttg
     2101 cttttcaagt gataataatc cgcgtgagct aaatcataaa cagaatgtat acccatttgt
     2161 ttcagacgtt ttgctgttcg tttgccgatt ccccaaaagt cagtgatatc tgagattttc
     2221 cataactttt ctggtacatc ttcataacgc cattcagcaa acatttgctt tccgtctttt
     2281 gagccattgt ctaaggctag tttagcaagt agcgggttat cgccaattcc tatagtcgtg
     2341 tagattccgg tctgctcaaa cacatcttta tggatcagtt tagccatgtc gtacgcattt
     2401 gttttaccaa agagtgccat gctatttgtt acatcaagga agctttcatc gacggaatag
     2461 acatgatggt tcgcttcgtc tgcatatttt ttatagatag aattgatttc tctgttcttt
     2521 tccatataat aggccatcct tggggcaaca atatataaat cacttggata ggggaaggga
     2581 agatctctgg cacggctgat attggtgatg ccgtaagctt tttttgctgc aggactggaa
     2641 gccaagatta atccgctacc ccgtgtttct gtcgaatcgg acggatagga catgactact
     2701 aatttagttg taaggggatc aagatccttt tcaacgcact ctacggaggc gtaaaaagat
     2761 ttgcagtcaa tacataaaat atctcttgaa ggttcttttt cataaacaaa taccggattt
     2821 tgattcgccc acataacaat cacctcttca acacaattag aacacacgtt ctctttccgt
     2881 gtcaataaaa aacagcaatt ttcaaatacg agcaaaaggc tcacatttgg aaattgctga
     2941 gaggttattt ttatctattt gtttttagct gagatcgttg atttttttat caaacatgaa
     3001 cagcttctgg atcgatcttt gttgtcccat ttccaatgtg tgcaacataa aattccggag
     3061 catagccgac aactttcatg tattcatcat agacattgtt tttgaaggca ggtatatttt
     3121 cttctttgac aagagcaatc gcacagccac caaatccagc tcctgtcatt cgtgcaccta
     3181 agacaccttc ttgtttttga gcagcatcta ctagtgtatc taattcgatc ccagttactt
     3241 cataatcata gcgaagagag tggtgagaat catttaacag ttgaccaaat gcttctaagt
     3301 tcccagcttc taattcagct tttgccttca aagtccgctg attttcagaa acggcatggc
     3361 gagctctgcg tatcaatgta tcatcgccga tttggtcaat agttgattca aattcttctt
     3421 catctaagtc tcctaaagaa gagataggca aagttttttg caaacgtgcc aaggcttctt
     3481 cgcattcgct acggcgttca ttgtattttg aatctgccaa ttcccgccgc ttattcgtat
     3541 tcatgatgac gattgcataa ccatctagtt tcactggaac catttcgtat ttcagcgtat
     3601 ttgtatctaa taagattgca tgatctagct caccgaagcc gatggcaaac tgatccatga
     3661 tacctgaatt gacaccgatg aattcatttt ctactttttt acctgtttgg actagttcca
     3721 agcgatctgt ttttaaatca aacagatctt ctaatacaac acctactaat aattctaatg
     3781 aagcgctact ggaaagtcct gcaccattag ggattgttcc ttccactaac aactcaaacc
     3841 catgatcgat tgagtgtcca gcttctttta atttaaggac cattcctttt acgtaatttg
     3901 cccagtttgc ttttttatca tattctagct catctaaagt aaaactaatg acaccgatat
     3961 cgtcaaagtt tgtggaataa actaatattt tattatcgtc tcgaggtgct gctacacctt
     4021 gcgttccata agtgatggaa gcggggaaga catggcctcc attgtaatct gtgtgttcac
     4081 caatcaaatt gatgcgtcct ggtgagaagt attgtgtagt ttttttaggt ccgaatgttt
     4141 cggcaaattt tgtgtttaag cttcttttga tttccatgat gagttattcc tttcttgttt
     4201 tgattttgaa ttctgtcact gtttcaaata ctgatccagc ttttaaatga atcgaaccaa
     4261 atgctgagaa gcgctctgca cctggagctg tttgagtctc aaatgtgatg ccaccgtgat
     4321 gtgccagttt tcggttgcgc atttctggtg tttctgtgcc gaaatttgca gtgaaaataa
     4381 ccacactaga agcatctgta gcaataccta cttggattct tttatcgggg ctagtcaagc
     4441 gagcggcttc tttacccaag ccggtttctt ttaagaaaaa tggatgatcg attccgtcaa
     4501 caagattttt ttgatccaaa tcactagcaa aaacggacgc taatttttta ggtatttgaa
     4561 aatcgaaagc acttcctgca gcattttctt ttacccctat aggaatacta tctgttcgca
     4621 aaggagcata agcttcgcta ttgagccaaa gctcatgttg atcgattgac tgagaagcat
     4681 ctcccgtcaa gttgaagtaa acatgatttg tcggattgaa taaagtatct tggtcactgg
     4741 tgccgcgaga ggtcactcgc cagatattat cttttgtcaa agtatagcgg atttccacat
     4801 ccattgtacc tggaaaacca tgttctccgt cgggacttgt agtagtaaaa atgacagaag
     4861 cttcattttc gccattcaaa atgacataag accatttttt taattcaaat ccaggagcac
     4921 caccatgcag attatgtcca gtttttggat ctgtggctaa ttggtaagtt tttccattga
     4981 gagaaaagcg gccattttcg attctccctg cggtgcgacc gatagaagca ccgatatacg
     5041 gatctttttc gatatattct tcagcggtgt caaatcccaa aactaattct cgatcatttg
     5101 attttaaact gacgattcta gcacctaaat cagagataga aagtgctagc ttgtttttgt
     5161 tctccaaggt tatcaagtga taaccttggc caaaatcttt ttctgtaatc ttcattttca
     5221 agatcctttc ttaatgggaa ggaactactg catcgtgaag ttcatcttcc attgtatcag
     5281 ctggagccaa tttgttttcc aattcctcaa cgatttgtgc atgttttttc tctgtcaatg
     5341 taatcttcca agcaaaaata atagcggaga aaatcatcaa agcagcagga gcatagaatg
     5401 cataagcttt gaaagtcgtt acgccatgag cagtgatatc agcagcagta gcattaccag
     5461 tcatcccaga agcgatagcg acaaacccta cgatcccgtt agacaaagca ccagcgattt
     5521 tatctagtaa agggcgaatt gatagagtaa cagcttcgtt acggatacca tttttccatt
     5581 gaccatattc aacagagtct gtgatcgtca tcaaagcgga taagaaaatc aattgttgag
     5641 ggaagtagaa caatactagc ccgatgataa cgattggcaa acttgaacct gcgatcgtaa
     5701 agaaaaagta agaagaaatc atcattgcga tccctccaag gtaaacgtag cgacgagaga
     5761 tgacgcgtgt aagcattggg aatagtggaa cagctactaa accaagtaag gcagcaacta
     5821 gaccaacgat ccaaaattca gttggttggc caagtacata tttgaagaag tagaataata
     5881 cggcagttgt tgctacattg gcaatcgcat aagcgatata agcaagggca agccacatca
     5941 actgatcatt ttgagcaact gctttgaaca catctttgat cccagctttt tcattttgct
     6001 gacggatcgc agtatcgact tcttttgtgc ccaaggcagt gataatcgca gtaccgcctg
     6061 aaataacagc agcaatcaca ccgaaccaga accagcctga agcgccttca atatgtttat
     6121 gagtaacaag ataggtaaag aaagtaacga tagggacaac gactagagta gtaccatttg
     6181 cacctagaga agagccgaaa cgagcaaacg tagcaaattt ttcacgttcc tttgtttccg
     6241 tactcaaagc tggaatcatt gaccaaaaag cgatatcctt aaatgaataa aaactatcca
     6301 ataagatgaa aacgattgca aataatatga taaataaagt cgtattatga gtagctaagc
     6361 caaagaaatt ggtaaagatt gctaccaaga gtacggaact aacaacgccg ccgataacta
     6421 accatggttt gaatttaccg tattttgtac gagtattatc gatgatgcca ccaatcaaag
     6481 gatcaaaaat aatttcaact aagcggatac ctacaactaa gcttgtaact gtagcaatca
     6541 tttttgcatt cgttgcctta tctgttcctg cgaacatctg gcttgtcacg aataccataa
     6601 aataagtgct taatgtaaca tagaatgtgt cgtgtccaaa agcgcctaaa gcgtaggaca
     6661 cacgttggat cattttgctt ttcataatct gtattcctct cctgttcaat atataatact
     6721 aagtaaaccg gttacaacga ataacaaaga tcttttaatg acttaaactt aacaagaaat
     6781 cgcttccttt ttaatatatt tctgtgactt ttatttaact ttttgttgca ttatactaaa
     6841 ggagtaagga gggatagacg tggagcaatc gattttttta aaaaaggaac atcaacaagt
     6901 agaaaaagaa ttgtattttg atttttgtgg tttctctaaa acgatgccgt ttcattcttt
     6961 tggtcctgct attcgcaatg attacatcct gcatgtcgtg atggaaggca aaggatctta
     7021 tcatgtgaaa aatcaacaat tccaattgaa aaaaggtgat ctgttcttga ttcgtccagg
     7081 agactcgacg ttttacttat cagatggaga agatccgtgg atgtattgct ggctttcatt
     7141 tggtgggagt gctgcagaag aaatcattcg gcagtcgctt ttcagagaag accaatacac
     7201 gatggtctct tctgacatac aaaaatatat cgacattatt ttagaatgca tgaaagatac
     7261 ggaagaaacc ttggaaaatg aattacagtt aacagcatta acctaccgtt ttttatcagt
     7321 ccttttaaaa gatggcggaa aggtcagcgg tggtgtacaa aaggttcatt cctctttagc
     7381 tgttgaaaca gtcaaataca tcgaggcaca ttatgctgaa aaattgtctg ttgaggaaat
     7441 cgcccagcat ttagcggtca atcggagcca tttgtcgcga gtattcaaaa atcatatggg
     7501 taccagtatc aaggaatatt tgatcggggt acggatcaat cgaggggcgt ttcttctttc
     7561 tctgacggat gaatccgtgg aaagtattgc ctatcaagta gggttcaata gtctagtcgt
     7621 atttagccga atgttcaaaa agagtaccgg agaaacggct acgaactatc gtaaacgtat
     7681 gaaaaacgaa aattttaaga atattgcttt ggataccttg aaaaaacagt tggaaaaaca
     7741 aaccgcagtt tcttggacaa cctgattagt ctggttaaaa tgacaagcat cggcaaaaga
     7801 aacttgtggc ggttatgcta cactgtaaag cgaagactaa aaaggaggaa aagggatgcg
     7861 tacacttatc atcattagcc atccaacgat cgaagaatct ttaaatcagc aatttttcaa
     7921 agaagcttct gctggtttac cagctacttg gcatcatttg gaatcaagtt atccagacgg
     7981 aaaaatagat atagagactg aactacagct cttggcccag catgatcgga tcattttcca
     8041 gtttccgttt tactggtata gttctcctgc tcatttaaaa atttggcaag ataatgtctt
     8101 ggaacgagca gcacatgtat taaaagggaa agaactagga atcgtattga caacaggtgt
     8161 ggcggaaaaa gaataccaag ctggaggaaa agaagaatat acgatttccg aatttttgag
     8221 gccgtatcag cgaatcgcaa ataaattcca tatgacctat cttccgccat tcgttttagc
     8281 acaatttatg tatttatccc aggaaaaacg ttgggagaag ctaattgctt atcagcagta
     8341 tctctcattg gaaggcaaac catcgctgac tcagcggatt gactggttta ttcaaagagt
     8401 acaggaaaat cagaaaatgc aggaagaaga atctgaaaaa caagcatata tcgtagaagc
     8461 actgactgag gcaaaagagc agatcgaaga cttgaccttt acactgcaag aaatgaaagg
     8521 aacttcatta tgacaaatga ttggctgatc caacaattag atgaattaag cgaaaaatca
     8581 gaatatcaag accgtgcgct cttatatgaa ataaagaaac tgatggctga gatagacgaa
     8641 cgaacaatgc agctgcaagg agaaatcgac ggacaatctt ggaatcatct caactggtga
     8701 aattttctta taaataacaa agataaaaat gtgcacagcg acaattattt gctacaataa
     8761 gaatatgaag cgtttgataa cgctattata cataaaaatt ggtggtgaaa aaaatgtatg
     8821 atgaaaatga gtggggagaa aatgttcgga tgacccgaag aagacagcaa aaacaggcag
     8881 ctcgtaagcg gatgatcatg attggggcag cagttgccgt gtttttgatc atagcaggag
     8941 gagttgcggg tctatatttt catggaaaaa attcgtcaga agcgaaaagt acgaccataa
     9001 cggaaacaac gacaaagaaa acagagacga aacaaacaag tgagtcatca actaaaacaa
     9061 aagagacaca aacttctgaa gaaaaaacag caaatggttt gccagagcaa cttcaagcaa
     9121 tcgtaaatga ttattcgaaa aagatcgaag aaaagacgcc tgcattgatt gaagaatacc
     9181 agacagaaat tcagggaaat caagagggaa tagctggttt atcagcagtt gctaaccaaa
     9241 aagcacgtga gcttcaagca atctccgacg aagggatcag aaaattacgt gcagcgtatc
     9301 aggcagcaga aaataaagac ggtgtagact tagatacttt gatcaatcaa ttatcggcta
     9361 attatacgaa tcatgtcgct aagatttccg atatttattt gcagactagc gcctctcttc
     9421 aagcagagtc aacaagcaca caagacacaa catcttctga ttctgagaca gcagagtcgg
     9481 tagaagcttc tgaaatgcaa gctcgaacaa cacaagacac aacagattcc tctgaaacta
     9541 ataccaacag cgcgacttca caggctgcga caaccgtagt tttggaagga gaaggtccaa
     9601 atcaaatagc cgaacgaact ggcgtaccag tagaaacgat cttgtcgctg aatgggatga
     9661 gcatggagaa ttatttcctg actcccggtg agacgctaaa attgaattga taaaagcaat
     9721 aaaaaagaac gaagcttcca ctaaggtgaa gcttcgttct ttttttattt tccggttctt
     9781 ttttcgcgta caaagtaatg gatcgtcatg taaagcaaga aaacgagtcc aataatcaat
     9841 gagattctcg tttcaggatt gataaacatg aaaatcaata cgatagctaa aaaagcaagt
     9901 gtcacataat tcgtataagg agatagtggc attttaaaag gatgatcatg cattttatct
     9961 gcgtgcttct ttttgaagcg aatctcgctg atcaaaatca ctacccaagg aaccatacca
    10021 ggcaaaatac ttgagctgta tacgaaaacg aaaatatttt ctgactggtt gatgaacatt
    10081 ggtagaagat aatttaagat cagaccaatt aagattccac ctgagattgc tacgacagga
    10141 taaaaaggcg caccgtgacg agatagtttc agaaattttt tagacatctt tccatttatg
    10201 gcaagtgtat agaccatacg acttgcgctg aaaatccctg aattgcaacc tgataatgcc
    10261 gctgtaatca cgacaaaatt aatcagtcct gcagcaaatg taatgccgac tttagaaaaa
    10321 gtttgtacaa aaggtgaacc aatctcactc aattgattcc acggatagat actcacaatg
    10381 ataaaaatag caccaatata aaaaatcaag attcgaccga tagtggactg gacagcttca
    10441 atgatcgtct ttttaggatt ctctgcttca ccagcagtca ttccgatcag ttcgattcct
    10501 tgataggaag caacaacgat cgataatgca aagaaaaatc ctttgacgcc tccagtgaag
    10561 aatccaccat ttttccaaag atttgaaatc cctactgcct gaccatgatt accaaatcca
    10621 aataagatta ccccaagacc tgcaatgatc atcaaaatga tcgttatgac tttgatgatc
    10681 gaaaaccaaa attctaattc gccaaacgcc ttgacagaaa taagatttgc cagcataaga
    10741 aacaagacga caatgattcc gggaataatt gccggcaaat caggccacca ataattcata
    10801 taagatccta cagcaatcac ttcacttatt cctacgacaa tatattgaaa aatattactc
    10861 caagcggtca agtagccagc cacaggatgg atgtactcgc tcgcgtaatt tgcaaaagaa
    10921 ccagtcgaag gatcaacgta aagcatttcg cctaaagcac gcataatcaa atacaaaaag
    10981 aatcctgcaa tcgcataggc tcccatcact gagggccctg tccaacgtat ggttgaggag
    11041 gcacccataa acagaccgac accgatcgtt ccacctagtg cgatcatttg gatatgacgg
    11101 gcactaagac ttcgagttaa cttttcttct tccatacaaa aactcctttc gttgttgagt
    11161 caacaagaaa aaataatcaa acgtttcaac acatgttttt cattattagg ttagccttat
    11221 tgaactcttg atgtgattct acagaaaatt ttcataaaat gcgaggaaaa ttcgaaaaaa
    11281 actttttcat cattctactt gaaaatgctt tccattttag ttaaactata tttaaataaa
    11341 aagttaggta aacctaaaaa ggagtgtaag atttggaacg tacggccatt gtaattcaag
    11401 acctttcagc cgcttatcaa gggaaaactg ttttgaaaac tatcaacttg acgataaatc
    11461 cacaaaaaat taccggtatt atcggaccaa acggtgcagg aaaatcaaca tttatgaaat
    11521 ctttgctaga gttgatacca gctactactg gtaaagtcac cttttctgga cagccggtaa
    11581 actcagtccg taaaaaaata gcctacgtgg aacagcgaag tgaattggat ctttcctttc
    11641 caattgatgt attaggcgtt gtacttttag gaacatatcc atctttacga attggacaaa
    11701 gacctgggaa aaaagaaaaa gaacgtgcaa gacaagcttt gaaaaaagta gggatggaag
    11761 aatatgccaa aagacagatc agcgaactat cgggtggaca gcttcagaga gtttttattg
    11821 caagagctct agcccaagga gcagaatgga tctttttaga tgaaccattc gtagggattg
    11881 atgcgttaag tgaacgaaag atctttgaca tcttgcagga attgaagaat tcaggaaaaa
    11941 cgattttgat cgtccatcat gatcttcata aagtagaaga atatttcgat gaggttattc
    12001 ttttaaataa acagctgatc gcttccggtc cagtacaaga agtctttaca tcgaagaacc
    12061 ttcaattggc ctatggtgag atcatcagac atttagtaaa aggaggagag aaaaaatgat
    12121 ccaatccttt attgatggat taatgaatta tcaattcctg caatacgctt tggtcacttc
    12181 catgcttgta ggacttgctt ctggagtcat cggttctttt attattttac gagggatgtc
    12241 tttgatggga gacgctatct ctcacgcagt tcttccaggt gtagccattt cttatatgtt
    12301 tggttttagt tacatctttg gagccactgc ttttgggatg ctaacagcag cagtgattgg
    12361 tttcgtgact cagcggagcc gattgaaaaa tgatacagcg attggaattg tctttagctc
    12421 cttttttgca ttagggatta tcttgatttc atatgcccag agtgcgacag atttgtatca
    12481 tattttattt gggaatgtct tagctgttag ggaaagcgat ttgttgctga cagcactcgt
    12541 tagtggaatc gtcttgatat ttgtcttttt cttttataaa gaattgaaga tcacatcatt
    12601 cgatccgaca atggcaaaag cttatgggct gaacacatcg ctgatccatt atcttttgat
    12661 gttctttttg acattagtgg ctgtagtcag tttacagaca gtaggaacaa tcttggtgat
    12721 tgccatgttg atcacaccag ccgccacggc ttacttgcta acgaaccatt tactgaaaat
    12781 gatcattaca gctgcaggaa tcggtatgct aagtgcagtt gtcggtgtgt ttttcagtta
    12841 tagttacaat tggccatcag gagctacgat cgtgttagca tgtaccgcat tttttatcct
    12901 tgctttttta ttttctccaa caaaaggaat tttatttcag aaaggaagca gatagatgaa
    12961 aacaaaaaaa tcactctttt taatcttggc agttagtttc ttggtattag ccggttgcgg
    13021 aaagcaggct tctgaccaag cggatgaagg aagtaaagaa aagttgtcag tcgtggctac
    13081 caattcgatc ttggcggaca tggcaaaaga ggtcggaaca gatcaaatag atatccacag
    13141 tatcgtacca gtcggaacag atccgcatga atatgaagta ctaccagaag acatcaaaaa
    13201 ggcaagcgat gcagatgtta ttttatacaa cggtttgaat cttgaaacag gtaacagctg
    13261 gttcgataac ttgatggaaa cggctaaaaa agaagaagga aaagattatt ttgcagttag
    13321 caaaaatgta gaacctcttt atttaactag cggtgaagaa catacaaaag cagatcccca
    13381 tgcttggcta gacctatcta acggaataaa atatgtggag gaaatcgcac gtatattctc
    13441 tgaaaaagat gcagaaaatg cgacactcta taaaaaaaat gcagaagcat atgtggaaaa
    13501 actaaaagaa ttagatacac aagcgaagga aagttttgct tctatcgaag agaacaaaaa
    13561 attattagta acaagtgaag gggcattcaa gtatttttca cgagcatatg atttgccagc
    13621 agcttatata tgggagatca atacagaaag tcaaggtacg cctgatcaaa tgaaagcgat
    13681 cattgatcag ataagagcaa aagaagtacc ggttttattc gtggaaacca gtgtagactc
    13741 aagaagcatg gaacgggtag caaaagaaac gggtttgaaa atctatgata aactgttcac
    13801 tgattccata gcaaaagaag gagaacaagg agattcttac tatcagatga tgaaatggaa
    13861 tattgaaacg atccatgaag gtttgtcaca aacaaaagaa agctgaaaag tgcaaggtct
    13921 agcttgagga gcagaccttg cattttttat aagagatatg aaaaaaaatt atgctgtttt
    13981 tcgtcgtaca gatacctttt ttgaagaaat tccgttatag ttagagtaag gaaggaaggt
    14041 gtggctatga attttacacg taaagataaa tatacgatta aagaagaagt ctttcgattg
    14101 atcagccagc aggaaaatac cgacaaggat caagaccttc aatttcttga agcagaatta
    14161 gctaaattat tcatagcaaa tcaaaatgag acaacagaac cagatgaaga gctgtacata
    14221 ggcaaatggt tgtttgatga atttcagcct ttagatgaaa ataccggtat cggttatttt
    14281 gtgttccttt ctccagtcgg cttcacacct ggcgtattag ttaaacaaaa cgacgaaata
    14341 accgtcaaag catggcctgc attattttca gtcatcaaag cagaaggagt aatcaaaaag
    14401 atccagtcgg cctattataa aagtcaattt attgccaaca gcaaattaat gaatatgttt
    14461 tataatcgtc tagtagaaat tgctaggcct gggaaaatcc cgcaaacatt cacgtacaca
    14521 cagcaaaaaa atctctatga agaagtattg tattcccgcg atctgtctga gttttcgcca
    14581 tgggaagagg taaggctcca tgaactgtta agtacaaaac cagtttcatt gaattttttt
    14641 aatgcattag ataacaaaag aaaattaatt tcaacaaatt attgggaagt atttgctgca
    14701 agaaaaaaat ggatggaaga aaagaagatt gccgatcagc agatccagtt tgtccagctg
    14761 aaaaaagaaa aactatatta tgggttatac agccgttaac agcaagcgag gaagaccatg
    14821 acattaaaag aagaaatcat acaagaaagc aaacgattag gaattgacaa aatcgggttc
    14881 accacggcgg aaccttttga ttcattaaaa gaatcgttgg aagaacaaaa agcagctggc
    14941 catacttctg gctttgaaca tccaaatatc gatgagcgtc tttatccaga aatgacattt
    15001 gatcagccga aatcgatcat ttccattgca cttgcgtatc ctactaggat ccatgaagaa
    15061 gtgcctaggg atgaaaaacg cgggcagttt gctcgtgctt cttgggggat cgattaccat
    15121 gatatactca gagaccgttt gaaccgattg atcgaattca tccaaacaag agcagaaaaa
    15181 tggcaaaaag aagaggaatg gcgtctagct ccgcaagtag atactggaga actgatcgat
    15241 gtcgctgtag cacagcgagc tggtttagga tttattggac gaaacggatt gttgatcact
    15301 gaagagttcg gttcatttgt ctatcttggt gagatcgtta ctaatattgt atttgagcct
    15361 gataaaccag gagaatttgg ttgcggtgac tgtatgcgct gcgtgactgc ttgtccgaca
    15421 aaagcattgc ttggtgacgg aagaatgaat gcccagcgat gtttgtctta tcaaactcag
    15481 acaaaaggga tgatgccgga agaatatcgt aaaaaaatgc gaaatgtcat ttatggatgt
    15541 gatatctgcc agttagtctg cccttacaat caaggaaaag attttcattt tcatgaagag
    15601 atggaaccaa aagtagacga agtatatcca aaattaaagc caatgctttc gatgtctaat
    15661 aaagacttca aacagcaatt tgggcattta gctggttcat ggcgtgggaa gaaacctttg
    15721 cagagaaacg cattgattgc tttagctaat ctaggtgatc gaacggcatt gcctgagatt
    15781 tcagaatgtt taacagatgt ccgccctgtc atcagaggaa cagcagcttg ggctattgga
    15841 cgtctaggaa caaaagaacc ggaaaaatgg cttgatgtat tagatgaaat attagctaaa
    15901 gaaacagaag aagtcgttat aaacgaattg gaacatgccc agaaattatt gcggagaaag
    15961 ttaaaataaa aaaatagtta acattattca aagtttttgt ccagtaattg ttgtaacaat
    16021 tactggtttt tttgtatttt ttacattttt ttaagagaat ttgataaaaa tcaaaaattt
    16081 ttttgctttt ttttatggat tattgcagtg aatgttacag aatctgcgtt tttcattcaa
    16141 aaataaaaaa acatagaaaa acgattactt tgcttataca acaaggcttt tatggttttg
    16201 tatcaaaaaa atatttgttt aaatgttaca aatattaaat aattcgttat tttgtcatta
    16261 aatgtcatgt ttcttttacc tatgaaatat ttctaaatga taaagttaat agcgtcgaaa
    16321 gaataaaaaa cggagaaaaa aaggagttta atatgaattc atttaagaaa attgttttag
    16381 gtacaacttt tgctgctggt gctactgcaa tgtttgtagg aactacaaat gctcacgctg
    16441 atgaagttta tacagtaaaa tcaggtgatt cattatctaa gatctcacaa aaatttgcag
    16501 gagacaactc aatgatcgat gcaattgctg aaaaaaattc gatcgcaaac atcaaccgca
    16561 tttatgttgg tgaacaatta acaatcccaa cttcaaatga ttcttcagca acaacagaaa
    16621 acaaaacaac agaaaataca gcatctacaa ctgaaacagc aacgcaagaa catacatacg
    16681 ttgcacctgt tgaaacagtt gaagtagctc ctgctgcccc agctgctgca acagcgccaa
    16741 cttcaagctc tgcaaaagaa tggatcgcac aaaaagaatc aggcggttca tacacagcta
    16801 ctaacggacg ttacatcggc cgttatcaat tagatgcttc atacttaaac ggcgactatt
    16861 cagctgccaa tcaagaacgt gtagcagaac aatatgtaac atctcgttat ggttcatggg
    16921 atgctgctaa aacattctgg ttagcaaacg gctggtacta agccgataaa aggaaaatga
    16981 tgaaaccgag aaacttgttt attcaagttc tcggtttttt t
//
LOCUS       DACDXO010000059        16665 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E23605_2017
            SAMN12639989-rid9881463.denovo.060, whole genome shotgun sequence.
ACCESSION   DACDXO010000059 DACDXO010000000
VERSION     DACDXO010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639989
            Sequence Read Archive: SRR10023389
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741914.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:42:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,972
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 33 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 17 of 73
            Pseudo Genes (multiple problems)  :: 10 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16665
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639989-rid9881463.denovo.060"
                     /isolate="E23605_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(157..864)
                     /locus_tag="IWM35_002128"
     CDS             complement(157..864)
                     /locus_tag="IWM35_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HAP9919969.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
     gene            complement(943..1281)
                     /locus_tag="IWM35_002129"
     CDS             complement(943..1281)
                     /locus_tag="IWM35_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HAP9919970.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            complement(1392..2354)
                     /locus_tag="IWM35_002130"
     CDS             complement(1392..2354)
                     /locus_tag="IWM35_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HAP9919971.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            complement(2369..3700)
                     /locus_tag="IWM35_002131"
     CDS             complement(2369..3700)
                     /locus_tag="IWM35_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAP9919972.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            complement(3708..4388)
                     /locus_tag="IWM35_002132"
     CDS             complement(3708..4388)
                     /locus_tag="IWM35_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HAP9919973.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            complement(4607..5086)
                     /locus_tag="IWM35_002133"
     CDS             complement(4607..5086)
                     /locus_tag="IWM35_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HAP9919974.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            complement(5213..6892)
                     /locus_tag="IWM35_002134"
     CDS             complement(5213..6892)
                     /locus_tag="IWM35_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HAP9919975.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            7440..8942
                     /locus_tag="IWM35_002135"
     CDS             7440..8942
                     /locus_tag="IWM35_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9919976.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESSETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVAVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            8955..9617
                     /locus_tag="IWM35_002136"
     CDS             8955..9617
                     /locus_tag="IWM35_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9919977.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(9718..9921)
                     /locus_tag="IWM35_002137"
     CDS             complement(9718..9921)
                     /locus_tag="IWM35_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HAP9919978.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
     gene            complement(10032..11216)
                     /gene="iadA"
                     /locus_tag="IWM35_002138"
     CDS             complement(10032..11216)
                     /gene="iadA"
                     /locus_tag="IWM35_002138"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HAP9919979.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            complement(11373..13112)
                     /gene="yfcC"
                     /locus_tag="IWM35_002139"
     CDS             complement(11373..13112)
                     /gene="yfcC"
                     /locus_tag="IWM35_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HAP9919980.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
     gene            complement(13885..14499)
                     /locus_tag="IWM35_002140"
     CDS             complement(13885..14499)
                     /locus_tag="IWM35_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP9919981.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
     gene            complement(15105..15482)
                     /locus_tag="IWM35_002141"
     CDS             complement(15105..15482)
                     /locus_tag="IWM35_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9919982.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
     gene            complement(15782..16663)
                     /gene="galU"
                     /locus_tag="IWM35_002142"
     CDS             complement(15782..16663)
                     /gene="galU"
                     /locus_tag="IWM35_002142"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HAP9919983.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
BASE COUNT         4829 a         3592 c         2846 g         5398 t
ORIGIN      
        1 ctgagccaga atgaggttca ttctgaccca gatttgattt tgtacaaccc atcagtcctt
       61 attgacaaag agcccttatt ccaaataaat ggtattcaat cagctaactc tcggtattag
      121 ctcagatcaa agcgctgatt tcacaacttc tttattttaa tctcttcggt tgccgccgaa
      181 caataagatc aagcgcaaca gctgcaggaa tgtcgaaagc gctgctgcaa cataagtcag
      241 tgctgctgca aataatacgt gtcttgccat cggtacttct tcttccgtca gtagtccgcc
      301 ttcactcaaa atttggattg ctcgccgaga cgcgttaaat tcgactggta atgtcaccaa
      361 ttggaaaagt aatgctaaag aaaaagcgaa aatcccgata ttgattaacg tagagttcca
      421 gctaagcaat acacctacca taatcaaggg gaacgaaatc atcgaaccaa agttagctgc
      481 tggaacaagt gctgcacgaa tcttcaatgg gacataaccc gaagcatctt gtaccgcatg
      541 tccgcattca tgagctgcta cacctatcgc agcaactgaa gtagactgtg cagttgcttg
      601 agacaaactc aacatcttat tacctgaatt gtagttatct gtgagatctc cggcaatttg
      661 ttgaacaccg acattattta tctgttgact ttgtaatata tattgtgctg cttgtgtacc
      721 agtcacattg tttttgcttc tcacttgatc gtattttcgg aaagtactat tgacgtacgc
      781 tgacgcaatt ccggaaataa ctaatccaag aatgaccaaa agaaatgtcg gatctaaaaa
      841 accatacatt ccataaccat acattttcgt ttcactcctt ttcgtttact ttattttatc
      901 attagtagta ggaaaagaat gtgaaaagac cttgaatatt ctttaattct ttttcacaaa
      961 ttccgatttc agtttcatcg gtccaaatcc ttctaccttg caatcgatat tatgatcgcc
     1021 ttctactaaa cggatgttct tgacttttgt tccttgttta atggcacctg acgcaccttt
     1081 tactttcaag tctttaatca ctgtcacgct atcgccctct aacagacggt ttccatttgc
     1141 atctaatacc actaatccgt cttctatgac agtttcttgt tcttctttgt tccattcgtt
     1201 cccgcattct gggcaaatca acaagcctcg atcttcatat gtgtactctg agccacattc
     1261 tgggcaattt ggtaatgtca tcttctttct cctttaaaat ctttagtgag cactattgta
     1321 acggaaaaag aagatcttga aaagatacat ttgtgatttt tactacaaaa ggcacgtttt
     1381 cgaacgaagt atcattctgt ttttctgctc ttatacgatt ggcatatatc ttggatctct
     1441 ttatagattt cctctacttc tgctaaatct tttgcattcg caccacttgc taatggatgg
     1501 cctcctccat gatggcgttt agccaattca ttgatgatcg gccccttaga acgtaaacgt
     1561 acgcggtaat accctgaagg ctgctgaaca aaaatccccc aagccagcac ttcgtcaatc
     1621 actccaggaa gtgaaacgat cgaagctgtc tctgaatcat cgattccata tttatcaaga
     1681 atttcttgcg gaaggattat tcttcctgca ccattttcat ccacctggat attttgataa
     1741 agataaccgg aaagtttagc gactttcatc ggcatttgtt caatctctct gttcaaagca
     1801 cttgcatcaa atggataatt tctcaatatc gaagcaatct ctaatgtacg ggacgtcgtg
     1861 gagggataca aaaagcgccc tgtatctccg acgattcctg cataaagtaa ccgtgcagcc
     1921 ttaacgttca tctttaaaga ttcaggaaac atcaaagcaa attctgcaat catctcacta
     1981 cagctacttg cttttgtatt tacccacaca agatctccat aagggtcatc attgggatga
     2041 tggtcgattt tgatcagttt ttctgccaac tgatatcttt gatcactgat tctgggtgaa
     2101 ttagctgtat cagtcacgat gactagcgct ccttggtaga agacatcttc aacttcttgc
     2161 atttctgcta aaaagcgaag cccttcaatc gtttcaccta cttgacgtat ttctttttct
     2221 gggaaacttt cccgtaatag ttcggctaat cctacttgtg atcctaatgc atcaggatca
     2281 gggcgcaaat gcctgtggat aatgatttta tcgtattttt ttattgtatc tactatttct
     2341 tgctgtactg tcatttcact cctgctttct aagaacgttc cattacttga caaacaacga
     2401 ttgctttgga aacgatggtg ttttcaataa acacttcgat atccagttta gcggaacgtc
     2461 tgccgatttc caatacacgt ggacgaatgt ctaattcgct ttccaattgg atcagccgca
     2521 aataatgtag gttcacttgt tcaatcaaga tatttcgttt ttggttcatc accattgttt
     2581 tttgtgcgac atcagaaatg atttcgctaa gtacgccaaa agaaatggtt ccaacgccat
     2641 tgaccatttg tggtgctact acgaatttga acttagggtt ttccaaacga tttccttcac
     2701 ggtcagtatc aatcgtatgg atatttccag atatctgatc cgaaatcgta tctgcaatct
     2761 gcggttgtcg ctggacaagc tgcatcgctt tcattacatc ttgccgggta atcaagcctt
     2821 ccaatgtcaa gtcatcggaa acgaccggca tgacttctag cccatcccag atcatttgat
     2881 ggctaacaga ggcaatactc attgttttct ttacactgat tggttctttc gtcatcaccc
     2941 gatcgatgat ctggtttggc gatttcccta aaacatcttt tgccgtaaca atccccatca
     3001 aacgatgatg gcggttgacg actggaaaac gcgaatgctg cgtttcttct gacaaacgct
     3061 gataatcaga aatcgtatct gtctgctgca aataatgagt cttttcagct ggcatataga
     3121 tatcactgac aagcatgata tcttttttga tcaattggtc gcttaacgct cggttgatca
     3181 tggttgcgac tgtaaatgta tcataagagg tgcgtaagat cggcatttca agatcatcag
     3241 ctagctttgc aatctcagca gtcgtattga atcctcctgt gatcaatact gcggcgccat
     3301 cttccaatgc taatttttgg acttccgccc ggttgccgac gatcattagt gatcctggcg
     3361 taatatagcg ttccatggca tgtttagtca tcgcgccgat gacgaattta ttcaagacct
     3421 tgtctaatcc tgccgccccg cctaaaacgt ccccttcaat gatcctgacg acctcgccaa
     3481 atgtcaaacg ttcaatatgt ttttttaatt tgcgttcgat acggatggtc cctactcgtt
     3541 gaattgttga aactagtccg acattttctg catctttgat tgcacggtac gctgttcctt
     3601 cactgacatt caattctttt gcgatgcttc ttacagaaat tcgattgcca acaggtaaac
     3661 tttcaatata ttctaaaatc aaatcatgtt ttgtcgccat tttctgccta cacttccagc
     3721 gtttccgaga cgctcattat ttttccttca ctatcttcca gtaactctac aaaagattcg
     3781 ggatcttgtt caatgacagg gaaagtattg taatggattg gaactacttt cttggcacga
     3841 attgtttgcg cagcctttgc tgcatcttct ggccccatcg tatagttatc gccaatcggc
     3901 aagaaagcga catcaatatc gaattcttct gcaagcaatc ctagatcact gaaataagca
     3961 gtatcccctg catgataaat cgtttttcct tctgcttgaa taataattcc gctaggctcg
     4021 cccatatact gaacgatacc gtctacttca tagcttgaac tatgctgtgc atgtacgaat
     4081 tttaccacgc cgaatggaaa cgcatgtttt ccaccgatat tcataccgaa tgaatctact
     4141 cctctgctgt tcgcataatc agcaatctca acaatactga taattgtggc ttgattttct
     4201 tttgcaatag gaatcatatc tcctatgtgg tcactatgac catgagtaat cagcaaccaa
     4261 tctgtcttta cttcttctgc agttaagtct gtcaatggat ttcctgtaat aaacgggtca
     4321 atcatcacgc gttggccatt ttccatttca attgttaaac aggaatgtcc atgatacgta
     4381 attttcatat tttacacctc gcgttttctc tgtatctttt ttattaaaaa cgatttcatc
     4441 acttgcacat caatgaaaaa atatagctgc taaatatatt ttcattggat tgatagtaca
     4501 gtatgtacaa agcaattgta acagataata caggatgcac caagtaccaa gcagatataa
     4561 tctcaagttt gaggtctctg ctctgcttag cttctcttct cgtcccttaa ctaattaaat
     4621 cagcgtaaat aggatggacg atcttttcta aatcggctac ttttatccga aggcttctcc
     4681 cgatttcacc gcttgacaca attacttctt ctttttcttt taaagaactg tctgctaaaa
     4741 aacaataatc aggatgtgcg agttttattc caataggggt gtttgctccg tggggatatc
     4801 ctgtatgcat taatacaaaa tccattccag gaaaaccaac tttacgatct ttcgtcactt
     4861 gtctcgtttt tttgtaatct aagtgatcgt tcaaaggaat caatgcgatg atcaccccag
     4921 ttttgtttcc tttcactacg agagttttac atatcaaagc tggatcaatc cctctttttt
     4981 gtagttcttc tgtgaaagat tcttctacca catgactaaa atcaagtggt tgatagctga
     5041 tcttttgttc ttctaaataa cgttcgactt ttgtttgact catcattgat tcactccctt
     5101 tattctatcg tttattataa agcgagggat gctgtattca aaaattattt gtttttcgca
     5161 aaaaaagagc aagacgagca ctctatagag aggttcatct tactcttttt gtttattttt
     5221 tatttcgacg ttgagtagct tccataatga ctggcaagat cactgggcgg cgttttgttt
     5281 gttcaaataa gtaacggcct aattgatctc ggatgtcttg ctttaatttg ctccactcaa
     5341 aatcattgct ttctaaatgt ttctcaacga ttgtttcaat catattgctg ctttctttca
     5401 tcagatcacg gctagctttt acgtaaacga atccccgtga agtaatttga ggttttgaaa
     5461 tgattttctt ttctcgccga ctgatagtga caaccgctac gaaaatcccg tcttctgaca
     5521 agatttttcg atcacgtaaa acaatgtttc cgatatcacc tatgcctaat ccatcaatca
     5581 ttacattttc tgcacttgta gttcctgcaa cactcatacg cttgttctta tattccagca
     5641 cgtctccgcg tccagtaata tagatatttt tgtatggcat ccctactgca tgagccaaat
     5701 ctgcatgagc agctaattca cgatactctc cttgaactgg cacaaaatat gttggtttca
     5761 tcaaattgat cattaattgc aagtcattag gattggcatg gccagaaacg cgcatattct
     5821 cagagatcaa cttcactgtt ccaccagctc gataaatgat atcttccgtt ttcgccacga
     5881 tggtttccat cgcgatgctt ggcgtagtag tgatataaac caaatctcct tcttcaatct
     5941 tgacattacg gtgtgtaccg tttgccattt tttgcagaga cttgatgggt tcacccattc
     6001 ggccagtttc taaaatgatc agttcttctg gatcatagtt tttcatgtct ttcaacgtaa
     6061 caagcagatc ttcacttggt aattgaagtt tttctaaacg catcgctgtg cgaataattc
     6121 gttcgaaatc ttgaccagtc aagacaactt ttcgttgtgc tttttctgct gcatccaata
     6181 cttgttgcac tcgttgcaaa ttacttgcta cacaagcgac gatgatacgc ccttcccaat
     6241 atttgatcgt atcaaatact tcatctgcta tttgggcttc agaagctaca ggctgaggat
     6301 tttctgcatt tgccgattca cttagtaagg ctaagactcc ttctttgccg atttccgcca
     6361 atcgtgcata atctgtttga tacattggga tagccgtttg gtcaaattta aaatctcccg
     6421 tataaacgat actaccttct tttgttttca gattgatccc cactgaatct ggtatcgtat
     6481 gagtggtacg gaaaaagctc actactgttt caccaaagtc tatttctgta tgctcatcta
     6541 ccacatggaa atccttaaat cctttggctg cttcattacg actgacattc aattttgcta
     6601 actcgatcgt caactctgtc ccgaatactg gtacagaaat ttttgacaac aagtaaggaa
     6661 gtgccccgat cgcatctgca tgtccatgtg ttaaaaagac accagctaca cgatcaatgt
     6721 tttcttctaa atacgtaaaa tcagggatta cgacgtcgat ccccaacaac tcattttcag
     6781 gatattttag cccacaatct aaaataaaaa tatcctcgcc cacttctgcg acatacatgt
     6841 tttttccatt ttcacgaacg ccgcctaatg ggataatttt aatggtactc acaaacttca
     6901 cctcttctta tgacatccaa gtttaaagat gtcgattttc ttctttatct aaatatatga
     6961 gccattccaa tgaagcatat gcaaaatcac tatttctcaa cttatggaga atcagcagga
     7021 aaattaccag cacatccgcg ttctgacagt tggatgggca ttttattttg gatatacttc
     7081 attaacaaaa tggatctttt gcgaaacgct atagccagaa tacttatata agtattcagc
     7141 tgttccccat tattatacaa caaataaact aaatcgctag tatcagcaat tgttagaaca
     7201 actatgcacg taaaaatccc tctgtagcat ctaggatttc ttttgctatt cctctttcat
     7261 ttcttcttcg tcggtttgaa acatactttt ttcattttac cacatttttt gcaataacta
     7321 tagtgacttt ttatatatac agaacctcat gttgatggtg tgcaaacagc ataagattta
     7381 agctagcttt aagataggtt gtctattatc aaatgcatct actaggaggg atgcaaaata
     7441 tgaattttat gaaacgtgca tggaaaagta caaaagtaaa atggggacgt tcattgttat
     7501 tgtttgccgt gttcacagcc attcttattt ttgtgctagc tggcctcacg attcgaagcg
     7561 ctgcagaaga agcagcgaat caagcacaaa aagaagttgg agcaacagtg actttaagtg
     7621 ccaatcgaga agcttcattt aaaaaacagg aacaaacttc ttcaagcggt tctaccactg
     7681 aagaacgtcc agatccaggc agtttttctc ttacaccagt ttccttaacg gacgcggaaa
     7741 aaatagctga attagataat gtaaagagtt attcctttga aagttccgcc tcagctatgg
     7801 caaaagatgg aataactgcc atcactagtt ctgattcaag tgaagacagt tcagaaacta
     7861 ctgatgcagg cggtgaacct gctggaggaa tgggcgggaa tccgcaaatg actcaagcag
     7921 actttcaaat cagtggtgtg tcagcaactg cacaaaatag taatttttct gatggaacag
     7981 caaaaatcac ggatggtgaa ggaatagatg acagcgacaa aggcacaaac aatgtagtca
     8041 ttgattctac tctagcagaa acaaatgacc tggcagtcgg agatactttc acaatcacga
     8101 gtactgaaga tgaagatacc acttatgaaa tgacgatcaa aggaatttat gaatccagcg
     8161 aaacaagcgg cagtatgggt atgaaattca acttcatgaa tccgtcgaat acactttaca
     8221 cttcttacac ttttgcaaat gaactaaatg ggacatctga cgataacacg atagattctg
     8281 ctatttacac tctatctgat ccagataaaa tggatgagtt tctcaaagaa gctgagaagc
     8341 tgatcgacac agatacattt agcttacaat caaatgattc catgtatcag tcaatgttgg
     8401 aacctttaaa caacgtggca agtttttcta aaaatgttgt tttactagtg gctgtagccg
     8461 gtattatcat acttactctg atcattatga ttaccattcg ggagcgtcgg catgagttag
     8521 gcgtcttgct atctttagga gaatccagaa gtaaaatcat tctccaattg tttaccgaag
     8581 tggctatttg tatgattctt gcattaggag ttgcttcgct tagcggaaac gtcgtcgcga
     8641 atgccgtggg acagcagcta cttgaccaac agacagaaac cacgacacaa aaccagcaag
     8701 ctcctggcgg tggacaaatg ccaggtaaca gacaagaaca aggcggtcaa cgcgatggtc
     8761 aacaaggaac cacgaataat ccatttgctg tttctgatca agtcagcgaa ttggaaatca
     8821 atgtcaaacc tgcacaattg ggactattag ccggagtagc atttggaatc agtctgtttt
     8881 ctgtcttttt agcttctata gggatattaa gattaaaccc aagaaaaatc ctattaaact
     8941 aaaggaggaa tttgatgtta cggacagaac aattgggatt ttggtatcaa gatccgcaag
     9001 atgcgcttta taaagatgta gaactgaatt tcgaagctgg aaaaatgtac gctattttag
     9061 gagcaagtgg ttctggaaaa acgacttttc tttcgcttat ttctggacta gatgcaccaa
     9121 aagaaggaaa ggtatactat caagatcgtt cactgaacaa aatcgggtta agaagttatc
     9181 gtaaaaatga tgtctcgatt attttccaag cttacaactt gcttccttat ttatcggctc
     9241 tagacaacgt catagcagca atggaaatca ctaagaccaa gcaagagaat tatcgacaag
     9301 ttgctttaga tcgactggct cgtgtaggaa ttgatgaaat attagcaaaa aaacaggtga
     9361 ccaaactttc gggaggtcag cagcagcgag tcgctatcgt tcgtgctatc tgctgtgaac
     9421 atcaactaat cgtagcggat gagcctactg gaaatctaga tgaaacaact tcacaagata
     9481 tcgtcaaact tttccaagaa atcgcccatg aacaaaatcg ctgtattatc atcgtgactc
     9541 atgagattga agtagcaaaa agctgtgatg aagtctttga actaaaaaat aaaaaatttc
     9601 aaaaaatcga agtttaaaaa acaggataat gatcaaataa aaaatccgaa ccatatctgt
     9661 ctcgcagata tggttcggat tttttatttg cttgattcaa ttagtaataa gtggttttta
     9721 ttttttttcg tcttttcgag aaaacttgtc tttgacttcg tcgcctaaat ttcgagcttt
     9781 ttctttgatg tcagcaccta gtgattgtgc tttcccttct gtttctttgc tcttgtcgtc
     9841 tgttgctttg ccgtatgctt cttttccttc accgactact ttatctttca tatctttaga
     9901 acgtcctttt aaatctgcca ttgtgcttcc tcctcttagt ttgtttattg atctaccttc
     9961 agggtaacat caacaacaga tacagacaaa cagaacgctc gttttctaaa aaagatagtt
    10021 agagctgttg attaaaaact tttttcgaaa gtcccccata ctttgacttc tttttcttgg
    10081 accattattt ctcctttagc gataactgta tcaatatcaa gcgtttcttt atccaagata
    10141 cacagatctg catcagcacc aatttcgatt tttccttttt tatccaattt caatatacgt
    10201 gctggattac tggttatagc tcgtaaggcg atttccaaag ggatactttc tttttgcaca
    10261 gcttctttga tccctactag caatgccttg gcacttccta ctcctaaccc taggaaatga
    10321 ttgttttcat caaaataagg caagcttcct tgtccatctg aggtcatcgt gaaattatct
    10381 aaagaaacat cttcctcgat caatcgtttc agtgctttgc tgaagcgaac ttcaccatct
    10441 gtcttctccc aaaaatcagg atcttcgcta gcagtgaagt caatcgtgcc accacgtttg
    10501 gcaaatgcca cgcaagcttc aaataattct ggtgtgcgat ttgcatgagt aggcaaaaat
    10561 tgtgtgatcg gaatttctgt ttcttctacg attcgtgtca atagctctag ctttcttttt
    10621 cctccgccta agtggacatt gatgatccca gcttttccgg ataacatgcc tcctacacga
    10681 gcatctgcta ctgcacgagc gaactcttca aaacttggct gagaagaacg gtgatcggat
    10741 actgcgattt ctccgattcc gataatttta tcaattgtca aaaagtcttt gatgatagaa
    10801 tctgttactg tttttaccgg caatcggtac gaaccttcgt atacataagt ggagattcct
    10861 tctgcttcca gacctttcgc tttgctgatc aacgcagtca tatctctacc ttctccgtct
    10921 gttcccaaac aaccgacaac tgtcgttaca ccagctgtag tcaattggct caatgtgact
    10981 tctggcgttc gattttgata accgccttct cctcctccac ctaaaatatg gaaatgacag
    11041 tcaatgaaac ccggggttag tacttttccc tcacctgaaa gttcttctac ttccactcct
    11101 tcgtagccgc cttctagctg ttcttctaca gccaaaatct tcccaccggc aatcagtaca
    11161 tccatttttc ctaagtaatc tggtgcaaac acttcaattt gtcgaatgat tttcatacat
    11221 ttttcctctt tctttaagta taactgatga taggatctta aaaaataatc caaacgatag
    11281 gatctttact cctttgattt taccgtaaaa tgacggttct tggagtcctt atctactgtt
    11341 tggattactt ttttatttac aggtctccag tactactgca gacctattgc aatggcaatc
    11401 atgatgaaaa tagttcctaa aacaaacagg aatccttgca tcttgatttg gaatcttgcc
    11461 cattttgccc attcgatccg tgcaacacct agcacaccaa tcaagctggc tgatacgggt
    11521 gtaaaagcgt ccataaaacc tgaaccaagc tgataagcaa gaactgctac ttgtctggat
    11581 acacctacta aatctgccag tggagccatg attggcatag tcaaagctgc ttgtcctgaa
    11641 tttgatgtaa caaccaagtt gaacaaactt tggaagatgt acatcgccca agcaccaatg
    11701 acagctggaa caccggtcaa cgccgtcccg atcccatgca aaatcgtatt caaagctgaa
    11761 ggaacagatg catcagatcc tcccaagaca agaagaatcc ctttggccat acctactacg
    11821 attgctgtac cagctaaatc agctgcacca gattggaaag aagaagcgat gtcattgact
    11881 ttcattccat ctaatttgaa aagaacagca atgattccag caactaatcc catcacaaag
    11941 aattgagaag cgatttctgg aatgtaatag cctttttgag tcacgcccca aacgatccag
    12001 acaaggacaa ctaacatctc taataagatc aatttatgcc caagcatgaa ttttttattt
    12061 tcatcagatg tctttttgat atgagaacgg aaataatcat ctgatttgta agtcaacgaa
    12121 ctttctggtt ttttacggat tttttctgca tatatcatca tataaccagc ggctaatgca
    12181 gtaacaacta cccacataat caatcggaat gtcgcaccag acaacacggg gattccagca
    12241 atcccttgag ccaccgctac gctaaacggg ctcatccaag aggtcgcatt tccgacttga
    12301 gatgcgacat aagtcacggt tactgctacg atcgagtcat atcctaacgc aatgacaaat
    12361 ggaaccatca ccattgagaa tggaattacc tcttctgcca tcccgaatgt agcgccaccg
    12421 aatgaaaaag caaagaataa tagcggtaaa gctaaacgct ccagtccctt agttttactg
    12481 ataaacgcat agatacctgc gtcgatcgct ccggtacgca taataatacc aaaagcacct
    12541 ccaacaacaa ggatcaatgc agcgatccct acagcagatc catacttatc tccagatact
    12601 agtccttcaa aaacaaaatt taagaaacca aagccgccaa aatcatctgt tccccatatt
    12661 ttggctgttt tatgcaattt tttagaagta tcatagatat tttcgccgta ctgatcgtaa
    12721 agcacatcat ctgtcaagga gatttcatct agtttttcct gcgtaagatt tttttctccg
    12781 tcagcaatga ctttttccaa tcctttttcc ggaacatcca gtttttcacg ttctgctgga
    12841 tgatcttgca gttcttctaa ttgatcaaac acatacgact tatccagctc gtaagcataa
    12901 cggaaggaat cttgacgtaa aacagtacga gtacttgttt cgccatttgc atctttgtat
    12961 tcaatatctt gcgtgctgaa ctttcctgct ggaacgacaa aagtcagtat ccatgcaaag
    13021 atgactacac caaaaataat gacatacgta tggggtgtcg ataatttagt gaagtcgcgt
    13081 ttcggttttc ctgaagaatt tgttttcttc aaatttattt cttcctttct tttttcagta
    13141 attttgatga ataaaaaatg gataatcaaa tgaattatac agtaaaaatg acatttccac
    13201 atgaataatt tattagaaaa tcactttttt ctattttgtt ttttttctcc tatgtatgat
    13261 tatttttttc tctttccatt tatattaagt attttatttc tctgtttttc cttaaaattc
    13321 tttttttatt tttagattag agaaaatctc gtcctgtttt tttcatttta caatcattga
    13381 aaaattgaat ttcattgcaa aaaaaataat aaacacttat aaatatccat tatctatcct
    13441 tcaatttttg tgtattttat ttctattaga aaaaggtatc ctgttggaca attttgttat
    13501 atcaggtaaa cttcattgca ataatgataa caaatgtact ataattactt tgtaaagagt
    13561 tttggggaag actctttcat caacgtgtat taagagacaa gaaagaatcc atatggattc
    13621 tttcttgtct ctttttccac tactttttat ttttctgaca ctacatattc ttataactca
    13681 tttctcataa ctgtgaaaca acttataaaa ataacaaact ttaaacaaaa caagcttaaa
    13741 atcaaagatg atccatctta aaataactaa aacaaaaaaa gtagtcgttg ttaatcctca
    13801 ttaataatcg ttttcttttt ttcaaacgct ccatttttat gtggaacatt atttttttaa
    13861 ggaactttgt caacatgtgg ataactattt tccacttgtg tcctggacta gctgttttgc
    13921 tgtaaaatac aaaacttgct gcagcaaaaa gatcgttgca agagaggctg ttccgcagat
    13981 cgctggatct agatttggca aagtcaagac tgcatcatgt aaattggcgt ggttgtccaa
    14041 aaccaaatca gcaaattcaa ataatcgcaa accactgcta tgttgcgatt tgatacttcg
    14101 tgacaaatca aatccagtaa caatgattaa tggatgacca ttttctttga tccactgagc
    14161 tagttcgatg attgccgggt ttcttccttc attcgaaaga agaagaaaaa tgtcttctga
    14221 tttgacagaa agctgatcaa taattgcctg tcctgcacct tctagcgttt catagacacc
    14281 tccggctgga ttttcaatca atatacttgg tacaagatcc ggggtttgat cccaaaattc
    14341 aaatgctaca ccacttagtg tgcggctagc atatatctgc aatctaccac cttgattaat
    14401 ttgtttcgcg atccaagtgc cagcttgaag catcagcggc tcttctatcc ttaatttctc
    14461 ttccaattgg tgataatagt caaaataaca taaactcatt tatctctccc gcctagctat
    14521 acttgacatg aatagaaaca gctgcctaat ctaaggaagt acataaaaaa acagtagtta
    14581 gacagtaaca catgtggtct ttgctgtttc aaacacaaaa aaggcattga acgaatacgc
    14641 ccaatacctt gacatagcaa ataaaacaac gtgcagacca tggaaaatat ctacataggg
    14701 gtagaatttt caccctagat taggtacaat aaattaggtc gatgcactgc attgactatg
    14761 gcaaaatgat cggtcagggt gtatgtgtgt gactggtcat tccctgattc ttagcgccaa
    14821 ctaagggtca gggagccttt ttttattcag ctgtttctat ctgaatttat tgtaagtaat
    14881 ctcttatcca agtgcaagca agaaaataca aaaaaacatt ggtatgattt atatatatat
    14941 aaataacata aaaaaaggaa tggcttatcc cgatacaaca agcattcctt taaaaaagat
    15001 tcttttatat aagtgaaaaa agcttttttc caccaattaa ataaatctct catctcaata
    15061 gtttatccat tattttatct ttttttgcgt atacctacta aatattaccg tcttcttctc
    15121 gttcttctac tttgttcgag agaatctgta ctagaaaaag tagttgtatg tctacctttt
    15181 ctgcctatcc gagactttct tttcatatat gggatataaa tgattagata cagtacagca
    15241 aaaaataaag caaatacgca agcttcccaa ctacctaacg ccaattcaac catcaaggta
    15301 gcaaccccaa cggatgctgc ataccagata aaaatcagaa acgtttcttt ttttctgacc
    15361 atgcgaaaca tgataaatag actaataagc aatagtggca acacaaatat ccaattgctt
    15421 ccttccatca gccacttgag tatatcaagc agcgactgtg caacagcgac taattttccc
    15481 atagtgaaca cacacctttt tttataatta aaaaattcgc ctttttattc tactatgaat
    15541 cttctggctt ggctttcatt tctcttatgt aatcattttc ttttatacaa cttaaaaaaa
    15601 aattaaacca ttagcataat attttctgaa tctattcacg aaagtctttt tctatctttc
    15661 gttttccacg actcaactga tctagagtca aaaaactaaa acgacagttg cgcaaatgac
    15721 gcacaagctg ccgctttaat aacaaatagc ctttcattct cagcaggcca cttatttcca
    15781 gttaactttc tgttcttagc tttttactca actggataat atatgcactt aatgaagtcc
    15841 caatttccga atgttgaagt ccatattcaa tatttgtctt catatatcct aacttatcgc
    15901 ctacgtcgta tctttttcct ttaaactctc tcgcatagac cgcctctgtc tggttaagag
    15961 tctgaagtgc atccgttagc tgtatctcgt ttcctgctcc tggttcttga gtttctaata
    16021 tctcaaatat tttaggagtc agtagataac gtccgataat ggctaaatta cttggtgctt
    16081 ttcccatttc aggtttttct acaaaccctt ttactctgca acgacccttt tcgctatctg
    16141 ttttaggatc aataattccg tatttagatg tttcattttc tggtacacgc atgacggcaa
    16201 cagcggaagc ttgtgtttct tcataatcca tcatcaattg ttgagtcaat gggatatcat
    16261 ctttcatcaa atcatccccc agcatgacga caaacggttc atttcctata aatgcttttg
    16321 cttgaagcac ggcatccccc agtcctcttg gatgagattg acggatgaag tgaagattga
    16381 cttgagtcgt ttcttcaact aaagcgagta attctgtttt ttccttttcc cgcaaattgt
    16441 tttccaattc gatatttgca tcaaagtgat cttcaatggg tcttttttct tttcccgtta
    16501 cgatcaagat gtcttcgatt cccgaatcca atgcttcttc cacaataaac tggatcgttg
    16561 gtttatccac gattggaagc atttcttttg ccatggcttt tgttgccggc aaaaaacgtg
    16621 ttcctagccc agcggcagga atgactgctt ttttgacctt cactt
//
LOCUS       DACDXO010000060        15997 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E23605_2017
            SAMN12639989-rid9881463.denovo.061, whole genome shotgun sequence.
ACCESSION   DACDXO010000060 DACDXO010000000
VERSION     DACDXO010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639989
            Sequence Read Archive: SRR10023389
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15997)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15997)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741914.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:42:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,972
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 33 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 17 of 73
            Pseudo Genes (multiple problems)  :: 10 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15997
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639989-rid9881463.denovo.061"
                     /isolate="E23605_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(434..856)
                     /locus_tag="IWM35_002143"
     CDS             complement(434..856)
                     /locus_tag="IWM35_002143"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HAP9919984.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
     gene            complement(870..2276)
                     /gene="atpD"
                     /locus_tag="IWM35_002144"
     CDS             complement(870..2276)
                     /gene="atpD"
                     /locus_tag="IWM35_002144"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HAP9919985.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            complement(2299..3201)
                     /locus_tag="IWM35_002145"
     CDS             complement(2299..3201)
                     /locus_tag="IWM35_002145"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HAP9919986.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMYQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            complement(3215..4771)
                     /locus_tag="IWM35_002146"
     CDS             complement(3215..4771)
                     /locus_tag="IWM35_002146"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HAP9919987.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEEALNSAIQEYKDMFLATKGNNSST
                     EDKLKSIQNA"
     gene            complement(4794..5336)
                     /locus_tag="IWM35_002147"
     CDS             complement(4794..5336)
                     /locus_tag="IWM35_002147"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HAP9919988.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNKAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYAYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMNYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLENMRNQLNR"
     gene            complement(5323..5847)
                     /gene="atpF"
                     /locus_tag="IWM35_002148"
     CDS             complement(5323..5847)
                     /gene="atpF"
                     /locus_tag="IWM35_002148"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HAP9919989.1"
                     /translation="MLNQLAIAEVGNAMLGNVIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            complement(5938..6153)
                     /gene="atpE"
                     /locus_tag="IWM35_002149"
     CDS             complement(5938..6153)
                     /gene="atpE"
                     /locus_tag="IWM35_002149"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HAP9919990.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVVALILVFGV"
     gene            complement(6206..6925)
                     /gene="atpB"
                     /locus_tag="IWM35_002150"
     CDS             complement(6206..6925)
                     /gene="atpB"
                     /locus_tag="IWM35_002150"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HAP9919991.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMVISLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            complement(7227..10070)
                     /gene="rnr"
                     /locus_tag="IWM35_002151"
                     /pseudo
     CDS             complement(7227..10070)
                     /gene="rnr"
                     /locus_tag="IWM35_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002418328.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
     gene            complement(10073..10819)
                     /locus_tag="IWM35_002152"
     CDS             complement(10073..10819)
                     /locus_tag="IWM35_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAP9919992.1"
                     /translation="MRQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKADYTV
                     YAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFAVRA
                     LEEESVIGGGFFCSPLSPVKTNVPENFEKYVRQVLKTAGKSEKEINEKAVAYRSLAEQ
                     QLMDIQDQAAIVESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLRWYPE
                     SGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            complement(10931..11167)
                     /gene="secG"
                     /locus_tag="IWM35_002153"
     CDS             complement(10931..11167)
                     /gene="secG"
                     /locus_tag="IWM35_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HAP9919993.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAIWMILLFVLAYLSSK"
     gene            complement(11285..11563)
                     /locus_tag="IWM35_002154"
     CDS             complement(11285..11563)
                     /locus_tag="IWM35_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAP9919994.1"
                     /translation="MEIKEEKNRLALFNDEQQEIGEMTWSDAGPDIMIIDHTFVDPAY
                     RGQKLAEKLVYTGVELARRDGKKIIPLCPYAKKEFEKKPEYQDVLRQS"
     gene            complement(11676..12644)
                     /locus_tag="IWM35_002155"
     CDS             complement(11676..12644)
                     /locus_tag="IWM35_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP9919995.1"
                     /translation="MVIFYFILILIIIGISYHFLSYFYAKKLVRAGVQRGYDWYEDTG
                     HRLMDPAAVTSVEKKRQKLEEECESFWEQGTPKTIKSYDGLKLAGQMFIHPNKQNKWV
                     ICVHDYRSTGKRDMSHIGKRYAEKGFNVLVPDLRAHGESEGEIIGMGWLDRLDLIAWI
                     QLILDEQPNASIILHGGSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSK
                     ITVFPKKMVMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAY
                     TIQNATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMN"
     regulatory      12859..12903
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            12927..13901
                     /locus_tag="IWM35_002156"
     CDS             12927..13901
                     /locus_tag="IWM35_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HAP9919996.1"
                     /translation="MRKVIIDCDPGIDDTLALSLAVKSPDIEVVAITVVCGNVPADIG
                     TQNVLKCLERCDRLDIPVYQGMEKPLKQPFISAQDTHGMDGLGETNFPMILRKQAEPL
                     HAVDFLADYFKEKTNTSVIALGPLTNIAFALKVNPNIGKHMDRFVSMGGTYKSHGNCS
                     PVAEYNYWCDPEAASYVFENLKQTIEMVGLDVTREIVLTPTILEYCCQMNPEEGEYLK
                     AITRFYFDFHWKQERILGCVINDPLAVAYFIEGAICEGFKSFTAVETQGISRGQTLVD
                     RYEFWQKPANSKIMTKVDTRLFFRKFLTVLLNAQEETIMKDLERLKMG"
     gene            13906..14481
                     /locus_tag="IWM35_002157"
     CDS             13906..14481
                     /locus_tag="IWM35_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP9919997.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQAGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
     gene            complement(14548..15687)
                     /locus_tag="IWM35_002158"
     CDS             complement(14548..15687)
                     /locus_tag="IWM35_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HAP9919998.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLAKHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHCIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
BASE COUNT         4372 a         3454 c         2861 g         5310 t
ORIGIN      
        1 atgttcttat ctcactctat cagtcctatt tgacaaagag ccaaaatctg agccagaatg
       61 tggttcattc tgactcagat ttgattttgc acaactcatc agtccttatt gacaaagagc
      121 ccaaaagtct tgtcgcagtc ccttattcca aataaacggt gttcaatcat ctgaccctcg
      181 gtattagctc aaaaaacggc aaaatatgag gaactatttt ccatttgttt ctctaatact
      241 caggacaaaa cgctgatttc acaacctccg atatacggtg ttccagaagt agcttcttcg
      301 aaaaataagc tgagatttca caaaaatttg agaagcaatt ttcgtaaatt ccttcttatg
      361 tcttgaagct gaccacttct gtcataacct ctttttactg ttcttaaaca cggataaaca
      421 tccgcatctc attttatgag tgcttagata ctttgatacg attgattgct cggtgcaacg
      481 caacagttgc ccgtttcaat tcgttcgtat cttgttttgc ttttgcttct tcgatttggc
      541 gttctgcgcg ttgttttgca cgttccgcac gagatacatc gatatcacgt tcacgttccg
      601 cactatcagc aacgattgaa acaacattat cacgaacttc catgattccg ccattaacag
      661 cgatccaatc gacgtgggta tctgaatctg ttcgttttac acgaacttca tcaatcgtca
      721 atggaacgat gattggtgca tgtttaggca aaatcccaag ctcaccagct gtcgtacgtg
      781 caacgacgat agctgcatgg tgatcataga ccaaaccggc aggagttacc acatttactg
      841 ttaaatattg atccatgggg cacccctttc tagtagccta gtgttttcgc tttttctacg
      901 acgtcttcga ttcggccaac acttcggaaa gcttcttcag gaagatcatc gtatttgcct
      961 tccaagattt cgcggaatcc tttcactgtt tcttctacag ggacatatga acctggaaga
     1021 ccagtaaatt gttcagccac gttaaagttt tgtgacaaga agaattggac tctgcgggca
     1081 cgagaaacta acactttttc ttgatcggat aattcatcca tacctaagat agcaatgata
     1141 tcttgtaatt cacgatatcg ttgcaatacg tgttggactt ctgttgctac tttataatgt
     1201 tcttctccca cgatttcagg tgctaaagca cttgaagaag aagccaatgg atctaccgct
     1261 ggataaatcc cttgttctgt taatttacgt tccaagttgg ttgtcgcatc caaatgggcg
     1321 aatgctgttg ccggcgccgg gtcagtgtag tcatcggctg gcacatagat tgcttggatc
     1381 gatgtgatcg aacctttttt cgtagatgtg atacgttctt gcaattgacc catttctgtt
     1441 gccaatgttg gttgataacc aaccgcagaa ggcatccgtc ctaacaaggc tgatacttca
     1501 gatccagctt gagtgaaacg gaaaatgttg tcgataaaca acagtacatc ttgaccttct
     1561 acatcacgga aatattccgc aatcgtcaaa ccagtcaaag caacacgcat acgtgcacct
     1621 ggtggttcgt tcatttgtcc gaacaccatg gctgtttttt cgataactcc tgaatctttc
     1681 atttcataat acaagtcatt accttcacga gtacgttcac ctacaccggt aaatacagaa
     1741 ataccaccat gttcttgggc gatattatgg atcagttctt ggatcagtac ggttttacca
     1801 acaccggcac caccgaatag tccgactttc ccacctttta aatatggggc aagcaaatcg
     1861 ataactttga tccctgtttc caaaatttct gtacttgtac ttaattcgtc aaaggctggt
     1921 gcttttttat gaatttcgct tctttccgca tcttctggga aaggtgtttc taaatcgatc
     1981 gtatctccta gtacgttgaa tacgcgacct aatgtttctt tacctacagg aacagagatt
     2041 gctttgcctg tatcaatgac ttccattcct ctttgtaaac catctgttga ttccatcgca
     2101 atggtccgga tcactccgtc ccctaattct aacgctgctt caagaacaac ttttgattta
     2161 ttttcgtcat ttttataaac aactaatgcg ttgttgatat ctggtaagga ttggtctaaa
     2221 gaaaattcca cgtcgacaac gggaccgatt acttgaacaa tcttgcctga actcatcttt
     2281 tttcctccaa tctcaatact attctagtgc cgaagctccg gcaacaattt cggtaatttc
     2341 ttgggtaata gccccttgtc ttgcacggtt gtaagaaacc gtcagctcat caatgatatt
     2401 agcagcatta tcagttgctg ttttcattgc tgtcatacca gcagcatgtt ctgctgtttt
     2461 ggcgtcaacg atcgcaccgt agatcaaact ttcagcatac tgaggcaata actgagacaa
     2521 gatttcttct tttgaaggtt caaaaatata ttcttgttcg aaagtagttg cttcttctgg
     2581 atctaagtct gagattggca gcattttttc tacacgaaac tgactagtta gcgaattgat
     2641 atgatggtta taacatacgt ataattcatc gaatacttcg ttttggtaca tagttgttgc
     2701 catatttacg attttgcgga cttcatcaaa gcttggctga tcagatagat tgcgcagctc
     2761 atatgctaga ttgataccgc gtgctttaaa gaagtctgca ccagttccgc caattgcgat
     2821 catcacgtaa tcatcgggtg attggtggtc ttcttctaaa atagacatcg tttgtttcaa
     2881 tatcgagctg ttatatccac cgactaaacc gccatctgca gtgatgacga tataacccgt
     2941 tttcttaaca ggacggctga gcaacatgct gttgtaattg atatcccctc taggattttc
     3001 tgaggcaata tcatttaact gagtagcagt caaatgcgta acgatttctc ttacttttgt
     3061 tgcataaacc tgaaacttct gtgaagaagc ttcagatttt gtcaacttag aagcagacac
     3121 catctgcatt gcccgcgtga tttggctcgt ttttttcgtt gaagcgatac gtgtcttgat
     3181 ttcatttaat gaagcaccca ttcatctcac ctcactatgc attttgaatg gatttcaatt
     3241 tatcttctgt agaggaatta tttccttttg tagctaagaa catgtcttta tattcctgaa
     3301 tcgcactgtt caacgcttct tcttctggaa gatcttttgt tgtacggatt gtttcaaaga
     3361 tatctgaatg attggtatcc agatattcga ataattcatg ttcgaagtct aaaatactgt
     3421 ctacagggat actatctaag aatccatgtg tcaaagcata caaaatcact acttgttttt
     3481 caacagctag cggtgcatgc aatttttgtt tcaagatttc tactgtacgg cgtccacgat
     3541 ttagttttgc ttgtgttgct gcatccaagt cagaaccgaa ttgtgtaaat gcttctaatt
     3601 cacggtaact tgctaagtct agacgtaaag tcccagcaac ttttttcatt gctttgattt
     3661 gcgcagaacc ccctacacga gatacagata gtccggcatc aaccgctgga cgagtacctg
     3721 cgtagaacaa gtcgctttct aagaagattt gtccatcagt gatcgaaata acgtttgttg
     3781 ggatataagc agagatatct ccagcttgtg tttcaacaaa tggtaaggca gtcattgaac
     3841 cgccacctaa atcatcactt aattttgctg cacgttccaa taagcgtgag tgtaagtaga
     3901 aaacatcccc tggataagct tcacgacctg gtggacggcg aagcagcaag gaaagttcac
     3961 gataagcaac agcttgtttt gaaagatcat caaaaatgat caagacatgt ttcccgttgt
     4021 acatgaactc ttcacccatt gcagtaccag catatggtgc gatataaagc aacggcgctg
     4081 gttgagacgc accggcattc acaacgattg tgtaatccat tgcgccatat tttttcaatg
     4141 tttcaacttg tgtacgaact gtagaatctt tttgtccgat tgctacatag atacagatca
     4201 tatcttgacc tttttggttg atgatcgtat cgatcgcaat ggaagttttc cctgttttac
     4261 ggtcaccgat aactaattca cgttggccgc gtccgattgg tacaagagcg tcgattgctt
     4321 ttagacctgt ttgcattggt tcgttaacag atttacgttg cataacgcct ggtgccattg
     4381 cttctactgg acgagctttg tcagtaacga tttcacctag tccgtcaatt ggctgaccta
     4441 gcggattaac tacccgtcca atcaaggcct ctccaactgg aacttccatg atctttcctg
     4501 ttcgttttac tttatctcct tcacgaatgg actcgaaatc gccaaggata atgataccta
     4561 catcatttga ttctaagttt tgcgccattc cgtatgagcc atttgaaaat tcaagcaact
     4621 caccactcat cgcgttttct aaaccatgtg cacgagcgat cccgtcacct acataagtta
     4681 cggtaccgat ttcttcgact gaaagcacgt tttgataatt ttcaatttgt tctttaatca
     4741 aggcactaat ttcttctgct ttgatggcca ttcgattcac ctacctcttt gtactatcta
     4801 tttaattgat tacgcatatt ttctaattgc gtgcgaatgc ttccgtcgat tactcgatgg
     4861 ttagcttcaa caatcgcgcc accaataata gaagaatcaa cgatttgttt taattcgact
     4921 gattgatagt tcattgtttt tgcaacattc ttttctaatt gtattcgttg ttcgtctgaa
     4981 agtggaactg ctgttgtgac acttcccaac agcaaacctt gatgttcatc atatcgacgt
     5041 tcatattctt cgatcatgaa caaaagatca tccattcggt tatacgcata aacaacttct
     5101 aaaaagtttt taacgattcc atcatagcca ctaacaagct tgtccatgat ttctcgtttt
     5161 tcatgaggtt ctagacgaac atcacttaac atattaccta atccaggtac ttgttcatag
     5221 atttgacgta agcttacgag atcttgataa atttcttctg ctttatttga atcgatcgcc
     5281 agttcaaata aagctttccc gtaacgtctt cctactgtat atttatctag tttcatttga
     5341 agaacctaga ccttcgatat attgattgat taatgattcg tgcatttctg gagataattc
     5401 tttgttcaag attttttccg cgatttgaag agatagttct gcaacatcgt cttttacaga
     5461 gttcagcgct gaatcacgtt ctacagtgat atcagcttgg gctttacttt ttagacgagc
     5521 tgcttcttct tgagcatcct tcaaaatatt ttggcggctt aattctccac tttctttcgc
     5581 atttttgatg atatcagctg catcagaacg agaggctaac aattgttgtt cgcgttcttg
     5641 ttccattttc gctgagttga tgcgagattg ttctgcagaa tccaaatcat tggcgatctt
     5701 gtcttcacgt tttttcaaaa tatcgctgat tggtccccaa gcaaaatgct tcaagagagc
     5761 cagtagtatc aaaaatgagc cgctgacaac gatgacattt cctaacatcg cattgccaac
     5821 ttctgcaatt gccaattgat tcagcatagg cgagccttcc tttctttgaa gtacttctag
     5881 ttttcatgta aaaaagcagt aacaaaccaa attgtttctg ccttaatact gattcaatta
     5941 aacaccaaaa actagaatca aggcgacaac tacccctaag ataggaacag cttcgactag
     6001 agctaccccg ataaacattg ttgtacggaa ttgaccagac atttcaggtt ggcgagccat
     6061 tgattcgatt gttttagaga taacttgacc gttaccataa ccggctccga ttgctgctcc
     6121 catgattgcg attgctgctg cgatataatt cataattgtt ttcctcctaa attagataaa
     6181 tagtagtttg tttttaaaac gaatattatt cttccacttc aatcttatgg ctcatataaa
     6241 ccatagataa agtaacgaag acaaatgctt ggatgcttcc gataaacaag gagaatgcga
     6301 tccagaccat ctctaatgga attgctaacg gcaatgagaa ccagcctaag cttattacca
     6361 tttttgcaat caaggttagc aaaacttccc ccgcataaat attcccataa agacgtaaag
     6421 caagtgttaa taagtttgta aattcttcaa tcagtttcat cggaaacata aaactatatg
     6481 gtttcaagaa cgaatttacg aaatatcctt tgaagcctaa ctttttcacc ccaaataagt
     6541 gagtaagtaa gatcattata aatgccaaag ttaaagtaac aaacgggtct gctgtcgggc
     6601 ttttccaaag tgttgtttcg ttaggtaaaa cgatttttgt tactaaaccg atgttgtttg
     6661 caacaaaaac aaataaaaac aatgtaaatg ccaacaaatg gaaattgctc aattcttttc
     6721 taggcagatt atctgttacg attcctcgac taaaatcaat gacccattct atcgcatttt
     6781 gtttgcctgt tggtttcaac ttaagatttc ttgtaaaaaa gtaagctaag aagaaaacaa
     6841 tcaaacaggt cagtaacacc attatacaaa ctgtaccatc aaaccaaatt ggtccaatgt
     6901 ggaacgttag cgagcgttca tccaatccat ttcacctctt ttccccgaaa ttcacataaa
     6961 atcaattcat gtgcgtatct ggttttttcc aatcggtttc taaccacacg gtatcatacc
     7021 accatgaaac agaaaattca aaacattttc atgaaaatta gacacttttt tatttcagtc
     7081 aaaaacgaag cgaatttttc tttatacacg gtgctcctga tctaagaaaa aacctatgat
     7141 aatcatgttt tatcagatta aatgctaaca aattttatca ttaagtaaaa gcaaaagcac
     7201 cgaatatgcg aaaaaggata atgaaattat ctagagaaat tttttaatgt tcgatcaatt
     7261 tgtcgatcga tatcttttcg tttcaaatct tcccgtttat cataagattt tttccctttc
     7321 gccagaccaa tcaatacctt ggcatagccg ttacgaatat agacttttaa aggaacaaca
     7381 gtgattccag tatttttcaa ttcattttca agccggatga tttgtttttt gtgtaataat
     7441 aactttctcg tgcgcaacgg atcatgatta aaaatatttc cttgttcata aggactgata
     7501 tgaacattat gcaagaaagc ttctccgttg cggacgcgaa taaatccatc ttttagattg
     7561 atccggctgt ttcgtatcga cttgatctcg gttccttgca agaccatccc tgcttccatc
     7621 gtgtcgatga tcgaataatc atggcgagct ttcttgtttt gtgcaattaa ttttccctcg
     7681 ccttttggca tagctgtttc ctccaactca ttttattttt tctttttctt ctttttaaca
     7741 cctttgtaaa atggcttctt cccttttttc ttaccgaacg tttgcttact atcgctatat
     7801 cgtttgttct tcttgccatt tctttctggg cgttttcctg cttgttttcc ttcgcgtttt
     7861 cctgattttc catttcgtgt ttttggccgt tccaacgctg taacctcttc tgcggaaaca
     7921 agttcaaaat ctactgctcg ttcatctgga tcagctttga ctacttttac tcgcactttc
     7981 tgtccgattt taaaagtcag accagttctt tcacctacaa gagctaaatg attttcaaca
     8041 aaatggaagt agtcctgttt caattcattg atatggatca atccctctac tgtattcggc
     8101 agttcgataa acaggccgaa tttcacaaca gagctgatga tcccatcgaa ttcttccccc
     8161 actttgtcca tcatgaattc tgctttcttc atggaatcta cttcacgttc tgtttctact
     8221 gcacgacgtt ccatcttaga actatgatct gcaatttcag gaagttccgt agcccacgaa
     8281 ctttgattgc tttcactttt atcttcactg taagaacgga tcagacgatg gacgattaga
     8341 tcgggatagc gtcgaatagg agatgtaaag tgtgtgtagt aatctgccgc taagccataa
     8401 tgcccaaaat tgtcttctga atagcgtgct tgttgcatgc ttcttagcaa catagtattg
     8461 ataactgcag cttctggttt gtcttctact ccttcgatca catgctgtaa atcttttggt
     8521 gtgatcgttc ctttcgttcc tcggaccaga atgcccaaag ctgaagcaaa atcaaagaag
     8581 cgctgcattt tttcttcttt tggctgttca tggattcggt aaataaacgg cagatttaat
     8641 ttattataat gttcagctac cgtttcattg gccgcaagca tgaaagattc gatcaagcgt
     8701 tcaccaattc ctctgcttct tagctggata tcatttgggt gtccttcagc atcaacaagg
     8761 atttttgctt cgcgatcctc aaaagaaatc gcgccacgtt tgattcgcat cgtttcaagg
     8821 attcgatgca attcacccat ttcttggaac ataggtacga gctgagcata gcgttgcatc
     8881 gtttcttcgt cttgatcttc taagatttga ttgacagctg tgtaggtcat ccgttctgtt
     8941 gtttggatca cacttgggaa aatatcatga gagactactt gtccatttgg atcaatctcc
     9001 atttcgcaac tcatggtcag ccttggaaca tggggattca gtgaacagat accattcgat
     9061 aaacgttgcg ggatcatcgg aatcactcga tcagtcaaat atacacttgt ccctctttca
     9121 tatgcttctc tgtccaattg actgccttgt gtcacatagt aagaaacatc tgcaatgtgt
     9181 actccaagaa aataattacc gttatctaat tttttaaccg taactgcatc atctagatct
     9241 tttgcatctt ctccatcaat cgtaacaatg agctgatttc ttaaatctct acgtccagtt
     9301 atatcctttt cgctgatttg atctggaact tcatctgctt cagacaatac atcatcagga
     9361 aatttcgtag ggataccatt agccacgaca attgagagga tatccatgcc agggtcattc
     9421 ttatgaccaa tcacttgctt gattatccct tctaaactag tcgcataccc tttttcaggg
     9481 taatgggtca gttcgatcag cacaatactt ccgtcaactg gacgaactcc ctctgctgct
     9541 gcatatactt taaattggtt gagctttcta tctttcggga taacaacgcc gtaaagatcc
     9601 gtttcttgta tttcttcttc tgaataagca acgaattccc ctgctaactg agtagtcgcc
     9661 cggtctcgaa tctcgactac tttcccctct gctccgcgat ccatatctgt atttgctcgc
     9721 ttcatgatat cgattgctac ggtgtctcca tccattgcaa aaccagtcgc ttcttttggg
     9781 atatagatgt catcttcttc gggatcaatc gttacaaatc caaatcctcg ttcatttgct
     9841 cgaaatgttc cttcgatcaa gattggtttt aaaggaagtt tcacttttcc ttttttattg
     9901 aaaactatac tctgttctct ttccatttga gcaatcgttt gtactagtaa tttaaaatca
     9961 tcgctttttt ggaaccctaa accttcagcg atttcttcca tagaaaaact gcgtttgcga
    10021 ctgctttcca tgaaaaagag aattttctct tttatcgttt cttttgtcat atttattcct
    10081 cattccaagg taatttttct aaaaattcca agacgtcttt ttctaattcg cgtcttgctg
    10141 taccaactgt aatcacatga ccactttcag gataccatcg aagtgtcact cgctgacggc
    10201 tcaacttgcg tgcagtttca aatacgccat tagggtcgat catttcatct ttcccagctt
    10261 gagctaaaaa tatcggctgt tggatatctg acaacctact ttctacgatt gctgcctgat
    10321 cttgtatatc cattaattgt tgttcagcta acgagcggta agcaactgct ttttcattga
    10381 tttctttctc tgatttacct gctgtcttca gcacttgacg tacgtatttt tcaaagtttt
    10441 caggcacgtt tgtttttaca ggagacaaag gcgaacagaa aaaaccgccg ccaatcactg
    10501 attcttcttc tagtgctcgt acagcaaaga tgccgcccat tgataatcct aatacggcaa
    10561 tatcagaaaa ctgctcagac tgtaaaaaat ggatggcttt tttagtgtct gcccaccagc
    10621 tttctgcttt ttcttgcaaa atatcatatg gctccattgt tccgtgtcct ttaaaaagcg
    10681 gcgcataaac tgtataatct gctttttcca aaaatcgtgc gagcattcga acatcgtttg
    10741 gacttcctga gtaagcgtga agcagcaaaa ccgctcgctt tccatgtttt gcataaaggg
    10801 gttttggtaa ttgtctcact cgtctcacct ctttcgcctc attttaccat agtttctttc
    10861 ataacttctt cttaagcgtt ctctttgcaa taaaaaaagc cgcccgccgt tttgcgagag
    10921 gcttattcaa ctacttcgaa gataaatatg ctaggacaaa taataagatc atccagatag
    10981 ctcccataac tgcagttgat cgctgcatga cagcttcaaa gccgcgagct ttttgtttgc
    11041 cgaatagttg atccgcaccg ccggtaaatg cactcgctgc actattttgc ttgcttggct
    11101 gcatcataat cgcaatgacc ataacaatcg ataaaacgat cacgattccc aaaattaaat
    11161 tatacatatc gcagcctcct tcccaaaaga tatcgtctct ttaactttat catagataaa
    11221 agtccgtgac aactctatat aaatggaata acagctgaaa gttcaaaact ttcagctgtt
    11281 attcttatga ctgacgtaag acgtcttgat attctggttt cttttcaaac tctttttttg
    11341 cataagggca taaaggaatg atctttttcc cgtctcgtct tgctaattct actccagtat
    11401 acactagctt ttcagctaac ttttgaccac gatatgctgg atctacaaat gtatggtcaa
    11461 tgatcatgat gtctgggccc gcatctgacc atgtcatttc tccgatctct tgttgttcat
    11521 cattgaataa agcaagacga tttttttctt ctttgatttc catatgccgc actcctcaaa
    11581 taactgatat ttttttattc ctaccttatt ttagcagtct cctgatctat aaacaattat
    11641 ttatcctcga attacttaca aaaaagaaga gaaaattaat tcatcgttat tctttgttca
    11701 ataaatgaaa aaatcactgt ccaataggtt ttcggatctt tcatcgatgc ttccaagtgt
    11761 tctgcttctg gaacaagcag cagtgcttta tcccctgccg tagcattttg aatcgtgtac
    11821 gctgcttctg tagggacaaa atgatctctt tcaccatgaa tgaccaataa aggcagatgg
    11881 ttgctgccaa gctgtctcgt agctgatgct tgctttaagg agtaaccggc gtatttctgg
    11941 gcataatgat tcgcatatct catgaccatt tttttaggaa agacagtgat tttacttaac
    12001 atatatcgga attctgcata aacagaaaca tatccgctgt ctaaaataaa tcctttgacc
    12061 gctgatggaa gtttttcacc acttgccatc atgatcgtag aagctcccat tgatccgccg
    12121 tgtaaaataa tggacgcatt cggctgttca tctaagatga gttggatcca ggcaattaaa
    12181 tctagccgat ctagccatcc cattccgatg atttctcctt cactttctcc gtgcgcacgt
    12241 aaatcaggta caagaacgtt gaatcctttc tctgcatatc ttttacctat atgagacatg
    12301 tctcttttcc ctgtgcttcg gtaatcatgt acgcaaatga cccatttatt ctgcttattt
    12361 ggatggatga acatctgacc agctagtttc aacccgtcat aacttttgat tgttttgggt
    12421 gttccctgct cccaaaagct ttcacactct tcttctaatt tttgtcgttt tttttctact
    12481 gacgtgactg cagcagggtc catcaaacga tgaccagtgt cttcatacca gtcatatcct
    12541 ctctgtactc ccgctcttac taatttcttt gcataaaaat aagaaagaaa atggtaactg
    12601 atccctataa ttatcagaat aagaatgaaa taaaaaataa ccattgtaca cccgccgctt
    12661 tctgccaaac tataatctaa ctattatcca atgtaccgat ccataggatt ctttattttc
    12721 ggcacacgta aattccattt ctttttcatt gtatcattta cttaaagttc tcctcaatag
    12781 atttacttgt aagagagcat ttacattcgc aataaaagcc agtatactaa tttcgtgcat
    12841 tataatcaag acgctcgtct tggactggtt cggaaacttc ccagaataaa aaactaagta
    12901 tctctaaaga aaggagattg gaaataatgc gaaaagtaat tattgattgc gatccaggga
    12961 tcgacgacac tttagcttta tcattagctg tcaaatcccc tgatatcgaa gtagttgcta
    13021 ttaccgtagt ttgtgggaat gtccctgcag atataggtac gcagaatgtg ctgaaatgcc
    13081 tggaacgctg tgatcgttta gacattcctg tttatcaagg aatggaaaaa cctttgaagc
    13141 aacctttcat cagtgcacaa gacactcatg gtatggatgg tttaggggaa acaaatttcc
    13201 cgatgatctt gagaaaacag gcagaacctc tccatgccgt tgattttttg gctgattatt
    13261 tcaaagaaaa gacaaataca tcagtcattg ctttgggtcc tttgaccaat atcgcttttg
    13321 ctttgaaagt caacccaaat attgggaaac atatggatcg ttttgtctcg atgggtggaa
    13381 cttacaaaag ccatggtaac tgttcgccag ttgctgaata caattactgg tgtgatcctg
    13441 aagcagcttc ttatgtattc gagaatctca aacaaacgat agaaatggtt gggttagatg
    13501 tgacaagaga aatcgtcttg acaccgacta tcctcgaata ctgctgtcaa atgaatcctg
    13561 aagagggaga gtatctaaaa gcgatcactc gtttttattt tgacttccac tggaaacaag
    13621 aaagaatttt aggatgtgtc atcaatgatc cgttagctgt tgcttatttt atcgaggggg
    13681 ccatctgtga aggtttcaaa agcttcactg ctgtggaaac tcaaggaata agtcgtggac
    13741 aaacattggt tgatcgttat gaattttggc agaaacctgc taacagtaaa attatgacaa
    13801 aagtagatac tcgtcttttt ttccgaaagt ttttaaccgt tcttttaaat gcacaagaag
    13861 aaacgattat gaaagatttg gaaaggttaa agatgggcta acaaaatgaa taaaaacaaa
    13921 atggcaactc gcgtacttac agtgatggct ttgtgtattg ggatcaatta tattggagga
    13981 acgattgctt tatggcttcg tttgccaatc tatctcgatt ctatcggaac gatttttgct
    14041 ggtgcattgc ttgggcctgt acctggaatg ctgacaggat tgtccagcgg tattttaagt
    14101 ggagtgacta cagatatttt ttctctgtat tattcgccaa tccaaattct cacaggcttg
    14161 cttgctggat tgattttgcc aagatatctc ggcaaagata aaaacagctt gaaactgtta
    14221 ttactcgctt tgattttgtc ggtacctgga acgatcgttt cttcgatcat taccgttcag
    14281 ttatttggcg ggatcacctc ctcaggatcc agtatgatcg ttcagctatt acatgggtta
    14341 ggtttaagtc agacaacaag tgtgatcttg gtacaggcag gtacagacta ccttgatcgt
    14401 ttgttgtcta tcgtcgttgt ttcattagtc atcactatgt tgccacaacg cttgatatat
    14461 tcaaaaagaa aaataggctg aataaacaga gctggattag caaaataatg ctaatctggc
    14521 tctgtttttt catttattaa aaaaatctta cgctaagtag gataaaataa ttccttgata
    14581 tttttcaatc atatccaaat aattatcgat ttcaacatac tcatctactt gatgaggcaa
    14641 agtaacgaca ccaggcccaa agacgacaaa gtcgaaggaa tggttagctt tcgtaaattc
    14701 ggctgcatcc gttgttgctg ccgctccaac taaaggtaat ggctggctga attgttgctg
    14761 aatgcaatga atcaaaggag aatctggatc agctttgaca ggtattttgt tgtaatcgat
    14821 catcaattcc aaatgatagt ctgtctcttg attcaattca ttgacgattg attgcagcaa
    14881 tgcaatgatt ttatcatttg ggtattctgg aatcgatcga atgttccctt ggagctgcgc
    14941 atggcttggg atgctgttga cttgattccc gccactgatc aatgtaacat tatggattgt
    15001 ttttcctagg acaggattct cgattgtttc agcgaggtga ttcatttcag catttgcttt
    15061 cgtaatgaat tcatttaggt gattgattgc attataacct tggtctggca tagagctatg
    15121 cgcttctttt ccgtgcgagg tgactgtata gttgattgat cccatgtgcg tatacatcaa
    15181 actgtaatta gttggctctc cgatgatcaa ggcatctaag tcatctacat agcctgcttt
    15241 cgtgagctgt tccccaccta gttcacccac ttcttctccg acagtggcta ataatttgac
    15301 tgttccgtta aatggttttc ctgactcttt cagttcgatc atggcaatca ccattgctgc
    15361 caagccactc ttcatatctg tagcaccacg tccgtacaaa cggttcccat ggatttctgc
    15421 tgcaaagggt gcataggtcc aagacgattc atcaccagcc gccacgacat ccatatgccc
    15481 agataggcct aacacttttc ctgattggcc cttttggtaa cgagcaatca aattatctct
    15541 accatctctg taagaaacaa tctctcctgt tatgtcatgc tttgccaaaa gcttattcaa
    15601 ataggctgct acttcacctt cgtttccatt cactgattta attcggatga tctcttgtaa
    15661 aatcgcgatt ttttcttctt ttttcatcta atcctctccc ttgccatttc tttttattgt
    15721 atttgagtgt gctcgaattt tcaaatagga gcaaccaaag taaaaatgaa gaaagagagc
    15781 cactttatga aaaatatgct gctacttccc tcctatttta tttatacgag aggacttgct
    15841 catagattcc tattctactg ttctatcctt aaaagctctt atatgatgct agttattgtt
    15901 agctttcctt tttcaaggat gatcttttct tgtaccattc tttgtaaaca tcatatacag
    15961 aagctagttg caaaaaaaac tctctacctg ctgatat
//
LOCUS       DACDXO010000061        15230 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E23605_2017
            SAMN12639989-rid9881463.denovo.062, whole genome shotgun sequence.
ACCESSION   DACDXO010000061 DACDXO010000000
VERSION     DACDXO010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639989
            Sequence Read Archive: SRR10023389
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15230)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741914.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:42:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,972
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 33 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 17 of 73
            Pseudo Genes (multiple problems)  :: 10 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15230
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639989-rid9881463.denovo.062"
                     /isolate="E23605_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            278..787
                     /locus_tag="IWM35_002159"
     CDS             278..787
                     /locus_tag="IWM35_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP9919999.1"
                     /translation="MVQGIERGVYVVDKIQFNQVIEEFIKVNNLMTHIQKRPIRITND
                     LKLSTSMIHLIDVIGNYPETSITELADRLGVTKGAVSQQIPTLEKMNLISISQKKGNK
                     KTKLVSLTKYGRKVYESHNYLHEELYSSINEALESFSPDQISTIKQILEKVSYTINEY
                     QVSLTRKED"
     gene            880..2042
                     /locus_tag="IWM35_002160"
     CDS             join(880..1156,1156..2042)
                     /locus_tag="IWM35_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAP9920000.1"
                     /translation="MSRRQRRTYSKEFKQQVVDLYLAGKPRAEIIRDYELTTSAFDKW
                     VKQAQTTGSFQEKDNLIPEQVELIALRKKNKQLEMENDILKQAALILGPKRQVIDTNK
                     HKYSISAMCKVLNISCQTYYYQAKPVEDESELEELVQEEFIRNRKAYGTRKLKQCLAN
                     LGLQVSQRRIGRMMKQRGLRSTYTLAHFKFHSSTCNEAKTANILDGTFLQEKSLEAIV
                     TDLTYVRVGKKWQYICLILDLFNREIIGYSCGEKKDATLVKEAFAKIPYSLNNVQLFH
                     TDRGKEFDNQTIDEILWAFNITRSLSKKGCPYDNAVAESTYKSVKVEFVHQYRFETLA
                     NLRLELFDYVHWWNTIRLHGTLGYETPISFRQQRLAQRILDNELGCDTGGEAP"
     gene            2322..3302
                     /locus_tag="IWM35_002161"
     CDS             2322..3302
                     /locus_tag="IWM35_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP9920001.1"
                     /translation="MKKIGIREVAKEANVSPTTVSRVLNDRGYLSEETKRKVFDAMKK
                     LEYYPNELARALFKNETYTVGLIFPTITNPFHAELIQDIEFELSSRGYKVLLCNSLNN
                     PTKEKEYLTMLRKNQVDGIIVGTHNKDIADYDIPGLPIVAIDRSLGENTVTVSCDNYR
                     GGELATQLLVNHGCQNILCIRGDSKIKLPANSRTLAYKDVLNKFMLEPLVLEVPFVKE
                     INEKNEIIKTYLETHPNIDGVFAGDDLLASLAINNLGSSGKRVPKEVKVIGFDGAQQT
                     LIYNPELSTIQQPIDQISKVAVKKLLNMIKGEKETDELSLPVKLIKKNTA"
     gene            3424..4842
                     /locus_tag="IWM35_002162"
     CDS             3424..4842
                     /locus_tag="IWM35_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-fructofuranosidase"
                     /protein_id="HAP9920002.1"
                     /translation="MEKLLIKSNNHQLYYQPKDVWVGDVMPYGKEGKFFIYHQRDTRN
                     PVPFGEPFGWALATTKDFVHFEDFGESLLRGDDNERDQFIYAGSVFEANGKVHAFYTG
                     YNREFLAQGKTSQVLLHAESDDFVHWEKSETALELEPQEGYDIRNWRDPFVVWDDEKE
                     EYLLILGARKGQDKHLQTGRLVEFTSKDLTNWEFKGDFWAPGLYTMFEMPELFKIGDW
                     WYLVYSEYSDGNKILYRMSQSLEGPWIKPKDEAFDGRAYYAGRTAYDGKRRVLFGWVP
                     TKDQENDKNNYLWGGTLVPHEVYQKEDFSLGVKPIDEVWNAFINLHDIADVEIETADT
                     REEVTLVKNSGTLFNLETTISFDENLSDFSLRLYKNLETDESYEYRFNLDEGRLTFDK
                     NPCYPWFQYMDKGLIRPISLKPNQSYKLKVIVDDNIMTLYIDGIALNARVHGCFGQAI
                     TITATNGSMTAINTKFSNEIKK"
     gene            4913..6382
                     /locus_tag="IWM35_002163"
     CDS             4913..6382
                     /locus_tag="IWM35_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9920003.1"
                     /translation="MDYKKIAEDVLENVGGKENISSATHCITRLRLILKDKSKANMEM
                     LEEIEGAKGVLFNSGQLQVIFGTGIIEKVYKEFVELTGAKEVSVSEMKDEGVNNSGNR
                     LQRAFKVFSDIFIPIIPAFIGAAMVLGIRSLITTPGMFGMDGSFADHNVFLNDLSKFL
                     GIIATTFDYLPVLVMYSATKRFGGNPILGLLVGFVMIHPGLANRNDFVMGTIKPDYWH
                     LFGLSIPAVAFQGGVFPAILTSWFLAKVEKITNKYSPQVLAYILVPTVTIVLANFALF
                     LVFGPLGNFIGNVLGGIIDFLYMKMGAFGAFVFAGALQPLVVTGTQHAIQGIEANLVA
                     TTGFNYIQPIWSVSIIAQGGGCIGMYLLFKKKSKDRDIAISSFIPTLVGITEPAIFAV
                     NLKYSIVPFVCSFIGAGFGGAFMKIFDVKAIGQGLTVLPGLTIANPVLPYIFGNLIAF
                     ILPIIFILAYDKYKVFIPEAKHRKFAEKTDSKKLPVQVK"
     gene            6392..6859
                     /locus_tag="IWM35_002164"
     CDS             6392..6859
                     /locus_tag="IWM35_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAP9920004.1"
                     /translation="MLNFFRKNEISVVAPATGVFEKLEKLSDPVFSKGMMGQGFAIDA
                     KNETIVSPIDGFVSSIFPTKHALGIKTKSGIEILVHVGIDTVELNGEGFDIKVQEGQK
                     VKAGDILMTINFVVFEKNHKTKDVIVVFPDYQDEIFIPKLEEVKQGQELFVLG"
     gene            6895..7767
                     /locus_tag="IWM35_002165"
     CDS             6895..7767
                     /locus_tag="IWM35_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP9920005.1"
                     /translation="MVFGAIEAGGTKFVCAIGDKDLNIFEKVSFPTTTPKETMNLVLD
                     FFNHHKDDLEGIGVGSFGPIDIHCESKTYGFITSTPKLAWQDFDFIGSLKKQLNIPTA
                     WTTDVNASCYGEYVSGNGRGLSSVVYYTVGTGIGGGAIQNGNFIEGFSHPEMGHMLVK
                     RHNKDNFSGTCPFHGDCLEGLAAGPAIERRSGKKGQEVTENDVTWEIEAYYIAQCAYN
                     TTLMLSPDVIIFGGGVMKQKHLKKKVQDKFIELINGYVAIPDVDNYIMTPKLLDNSGI
                     VGCLALARDVKEKS"
     gene            7848..10382
                     /locus_tag="IWM35_002166"
     CDS             7848..10382
                     /locus_tag="IWM35_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP9920006.1"
                     /translation="MKKKMKDEVLEYIKNNLKYYDFSAQDIAMKFCIKRNVASHYLNQ
                     LFSDGKLLKNDSVRPVMFKYNQQKPKDCFSKFIGADISLKSTIDKCKATVMYPPNGLP
                     LIIKGNSGVGKSFLASLIYQYALDRKVIHNDAKFVVVNCADYANNPELLSAVLFGYKK
                     GAFTGAEKDTAGMLSSANGGYLFLDEVHNLSAENQEKLFLLMDSQKYRKLGESVQWEY
                     ANVRLILATTEDKNTSLLATFRRRIPAEITLPDYNNRSTSEKIQLLFEFLKDEAITID
                     SKIFCSIELFTDLLSKTFEGNVGELRNEIKLLCAEGYLGNKRKSSIYLGDKLDSGFWI
                     DPEISIDLKKMFLKSLSEIDLIELFQNHISIEESMSQLRDRILKECAGSSEYNYLESD
                     EFIALRNYVVNKISPIIDSTGLCLLDEFLADISLFIMFIDVIEETSRNFLLSKFRKFR
                     MKNDKQKLLANEIWSSLEVEESKQELLLKWILFLVKKFYVKIPETHCLIVMHGKITAS
                     AIARETNKLLNTYVYEAFDMPIEGETSDLINLINNFCKSIDTTNGLILLVDMGSLEQM
                     YEKIESNVIGDLVILNNVSTALAIECGIQVCQKKPISHFYQMDFDSFQVKVQYYKGLS
                     QKKNVIVSCLSGEGISEKVKDILKRYLDNQVEILIFDFNALKKIAKEKDTMIFKNTIC
                     VLSTTEFYIQGIDCLNLENLINGNQTLEKLNKYMDSDSCEFCLNELVKLFTIEGASTK
                     LRFLDSKKVFNEIEKVLYLYEKYYDVKIPSFLRINLFLHLSGMIERIMIGDGITNEHL
                     KEGAQLFDTFLNVSKDFFSEIKDIYCIEIPKGEYELIYTIFEQTIF"
     gene            10538..11311
                     /locus_tag="IWM35_002167"
     CDS             10538..11311
                     /locus_tag="IWM35_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP9920007.1"
                     /translation="MAFWQTFLVVLYGFFINWDKEGPKIGTSQPVVAGFLTGLIMGDI
                     QTGLYIGGTLQLMTLGITSFGGASVPDYQTAALIGTYLAISTNQDASIGITLAIPVAM
                     LIVQLDVLKWSTNIFFQQKAEKFAQKEDYQKLEWMQYLASINTSFVSGIPVLLTVILG
                     PTLVGSAINYIPEWVTGGLKVAGGLLPAVGIGMLLKYLPTKDYFAYLLVGFVLAVYFQ
                     IPILGISLLGAAIALIVYKNRSNNTQVAPAATGGFDEDE"
     gene            11304..12095
                     /locus_tag="IWM35_002168"
     CDS             11304..12095
                     /locus_tag="IWM35_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP9920008.1"
                     /translation="MNKKLTKIDKQVIFRWILNGATSLNYEKMEGLGYAYSMLPFIKD
                     TYKDDPEGMKQSVLAHLQFFNTTPYTAPYIIGMNVGIEKEEKNKSLEAVSALKTGLMG
                     PLAGIGDSVFVVIPWTIFGAIAANMALEGSFVGIILWILASILLRITAYPLYLAGKES
                     GASLLTQIETKLKSITDAVSILGLMVVGALIATVVKVNVGFEFKQGDLTMTGNEIFDQ
                     VMPGLLPACVVAIVYYLLGRKVKPVYVILLILVVSIALRALNILK"
     gene            12105..12596
                     /locus_tag="IWM35_002169"
     CDS             12105..12596
                     /locus_tag="IWM35_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP9920009.1"
                     /translation="MDEKMKGIIHVRIDDRMIHGQVATQWTNQLSANRIMVINNEVAN
                     DDVKKAVVRLAAPPNVRTSIITREVAVKNILSGKYEGQKVLIVAVSPIDVKFLIDNGL
                     PITSVNVGNLSRRNGTERIRPTINITEEEREAFKELISDGVEVTVIQTPRDSKEFLKD
                     FIY"
     gene            12614..13024
                     /locus_tag="IWM35_002170"
     CDS             12614..13024
                     /locus_tag="IWM35_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP9920010.1"
                     /translation="MEFIITSHGNIAIGFVDTLKMFFGDNLNCHVVTLGDAGIEEFRE
                     NIEKIVSQVKNQEVMVFADLIGGTPFNEFAKRSEVFQNGFQLLGGMNLGILIEAVNLR
                     LQGKKSDDIIENLRNMNTIACLADMQRHTNEEDE"
     gene            13028..14428
                     /locus_tag="IWM35_002171"
     CDS             13028..14428
                     /locus_tag="IWM35_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-fructofuranosidase"
                     /protein_id="HAP9920011.1"
                     /translation="MQKIFYKPKDFWFGDCMPFYKDGTFYLFHQRDTRNPKPLTDPFG
                     WSLVTTTDFVKFEEYGEVIHRGGNDDIDQYIYAGSIVQGINEYLALYTGHNRKAKLDG
                     KTSEVLMIASSKDLIHWKKDGEAAKLVPQEGYDKNDWRDPFAIFDQDHGKYVLILGAR
                     KPASKKKPTGRLVYFESEDLKDWNFKGDYWANNEFNMIEMPDIFEIDGTWYLLFSEYC
                     EDKKTKYRVGAGLFSDWKSPVDEAFDGKAYYAARTVGTNETGRFLTGWVATKEGENDQ
                     NNWDWGGALVPHQIIQKTDKTLGVKLPEKIIENFKEITPFSSFKLEKEFGKKERVIVP
                     KIEDYFLFETTLTITSLTKEFALKLFVDSDTQEGYEFRISLINNQLTFGKTPNYPWPQ
                     MFDKGLQRPLFLEIGKEYELKLIVDDTIAVLYIDNVALSTRMYDKVGTALSMTVTEGA
                     IKVDKLDYSINYECGF"
     gene            14451..14825
                     /locus_tag="IWM35_002172"
     CDS             14451..14825
                     /locus_tag="IWM35_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HAP9920012.1"
                     /translation="MKLNGLQHIGIPTKDFGASKIFYESLGFSLINEEMNVGSKVGFF
                     ELNGTIIEIWEDKTTECRGAIDHLALDTDDIDEVFNSIQDLGYELLDKNIMELPFWEN
                     GIRYFNFYGPNHEIIEISQMKK"
BASE COUNT         5286 a         2135 c         3046 g         4763 t
ORIGIN      
        1 ttttttattt gtctcgctta attatacaaa cggcgtgata aaaaacgaga aatgaattat
       61 aggtttagac ctttatcatt tctcgtttta ttttattgta tcaaggctct atcataagtg
      121 gtaaaatcta cttttaaatc acatgtaatc attgataagt ccagtgtttt agcggataat
      181 cagcttttta tttatttttt cttataaatt tgataaatag aaattagtat agccctaaac
      241 aaattatttg tgttttattg tttagggcta tactatattg gtacaaggaa tagaaagagg
      301 tgtttatgtg gttgataaaa ttcagtttaa tcaagttata gaagaattca tcaaagtaaa
      361 taatttaatg acccatattc aaaaacgacc aatcagaata actaatgatc taaaactcag
      421 tacgagtatg attcatttga ttgatgtgat tggaaattat cctgaaacta gtattacgga
      481 attagctgat cgattaggcg ttaccaaagg agctgtatcc caacagattc ccactttaga
      541 gaaaatgaat cttataagca tttctcaaaa aaaaggaaat aagaaaacta aacttgtatc
      601 gttaacaaaa tatggtcgaa aggtgtacga atcacataat tatttacatg aagagcttta
      661 tagctcaata aatgaagcgt tagaatcatt ctcaccagac caaatttcaa ccattaaaca
      721 gattctagaa aaagtatctt atacaatcaa tgaatatcaa gtaagtctga caagaaagga
      781 agattgacat gaaaaaaatg atttttgaat cggctacact agactagaca gaaaaaataa
      841 ggtgtgtaaa ataaatgaaa acacactgga ggaattttta tgtcaagacg tcaacgtaga
      901 acctattcaa aagaattcaa acaacaagtc gtcgatcttt atctcgcagg aaagccccga
      961 gctgaaatca ttcgagatta tgagctgact acctcggcct ttgataagtg ggtcaagcaa
     1021 gcgcaaacta ctggctcgtt tcaggaaaaa gataacttga tccctgaaca agtagaacta
     1081 attgcattac gaaagaaaaa taaacagctt gaaatggaga atgacatttt aaagcaagcg
     1141 gcgctgatat tgggacgaaa agacaagtaa ttgataccaa taagcacaaa tactcaatat
     1201 cagcgatgtg taaagtgtta aacatctctt gccaaaccta ttattatcaa gccaaaccag
     1261 ttgaagatga atcagaactg gaagaactcg ttcaagaaga atttattcgc aatcgaaaag
     1321 cttatggtac caggaagctg aagcagtgct tggccaatct aggtcttcaa gtgagtcaac
     1381 gcagaatcgg ccgtatgatg aaacagcgtg gcttgcgttc aacatacaca ttggctcatt
     1441 tcaaattcca ttcttctact tgtaacgaag ccaagaccgc caatattcta gacggaacct
     1501 tcctacagga aaagtcatta gaagcgattg taaccgacct tacctatgtt cgtgttggga
     1561 agaaatggca gtatatctgc ctgattcttg atttgtttaa tcgagaaatc atcggctatt
     1621 catgcgggga gaagaaagat gcgacgttag tcaaggaagc ttttgcaaag attccgtact
     1681 cgttgaataa tgtacaactt tttcacacgg atcgtggaaa agaattcgat aaccagacga
     1741 ttgacgaaat tttatgggcc tttaatatca cgcgttcctt aagcaaaaaa ggctgcccct
     1801 atgataacgc cgttgcagaa tccacctata aatctgtcaa agtagaattc gttcatcaat
     1861 accgctttga gacattggct aatttgcgat tagagctatt tgattacgtc cattggtgga
     1921 ataccatccg gctgcacggc acgttaggct atgagacacc gatcagtttt cgacagcaga
     1981 gattggcgca gcgaatcctt gataatgagc tcggatgtga taccggtggc gaggcacctt
     2041 aattatttga atgagcttct gccttagaga atcagattcg aggaggctcg ttgtcaaggg
     2101 cagtcggaac accagcagca agcttacgca ataccttata atttttgtca aaaaaagtgt
     2161 tgccattcca catatatctt taaacagcta ttgccagata aaaataaatg atattatata
     2221 tataaatact tatccgaaaa ggggaatgag agagtcaagg ttcttggaat gtcgaatagt
     2281 cgagtaggta gattttctat ctacaacctt gttgtttaaa aatgaaaaaa attggaatta
     2341 gagaagttgc gaaagaggca aatgtttctc ctacgactgt gtcgagggta ttgaatgatc
     2401 gtggatattt gagtgaggaa acaaaaagaa aagtttttga tgccatgaaa aaattagaat
     2461 actatcctaa tgaactggcg agagcacttt ttaaaaacga aacatatacc gtaggtttga
     2521 tttttcctac aattactaat ccatttcacg ctgagttaat tcaagatatc gagttcgagc
     2581 tttccagcag gggatataaa gtgttgctgt gcaatagctt aaacaatcca acaaaagaaa
     2641 aagagtactt gacgatgttg agaaagaatc aggttgatgg aattatagta ggaacgcaca
     2701 ataaagatat tgcggattat gatattccag ggctacctat tgtagcgatt gatagaagtc
     2761 ttggtgaaaa tacagttaca gtatcttgtg ataattacag gggtggggaa ttagctactc
     2821 aactacttgt aaatcatggc tgtcagaata tactgtgtat tcggggagat agtaagataa
     2881 agctacccgc gaatagtagg actttagcat ataaagatgt tttgaataaa ttcatgctgg
     2941 aaccgttagt cctagaagta ccatttgtaa aagaaatcaa tgaaaaaaat gaaataatta
     3001 agacatatct ggaaactcat ccaaatattg acggggtttt tgcaggagat gatttattgg
     3061 cttctttggc gattaacaac ttaggaagtt cgggcaaaag agttccaaaa gaggttaaag
     3121 tgattggatt tgatggagct caacaaacat tgatttataa tccagaatta tcaactattc
     3181 aacagcctat agatcaaata tctaaagtag cagtaaaaaa attattgaat atgattaagg
     3241 gcgaaaagga gacggatgaa ctttcacttc cagtaaagct cataaaaaaa aatacagcat
     3301 aagttaaatc ggcgaaaaat tccttcgtcg atttaaaaat atttttatga aacgggttga
     3361 catatgaaac gggttgacat atattagttg tgaagataaa aaccttttca ctggaggaag
     3421 aaaatggaga aattattaat taaaagcaat aatcatcaac tttactacca acctaaggat
     3481 gtttgggtcg gagatgttat gccttacggg aaagaaggaa aattctttat ttatcatcaa
     3541 cgtgatacta gaaatccagt tccttttgga gaaccgtttg gctgggcttt agcaactacg
     3601 aaagattttg ttcattttga agactttggg gaatctcttt taagagggga tgacaatgag
     3661 agagaccagt ttatctacgc tggttctgta tttgaggcaa atggaaaggt tcacgcattt
     3721 tatacagggt ataatcgtga attcttagcg cagggaaaga catctcaagt gttgcttcat
     3781 gctgagagtg atgattttgt tcattgggaa aaatcagaaa cagcattgga gttagaacca
     3841 caagaagggt atgatattcg aaactggaga gatccatttg tcgtttggga tgatgaaaaa
     3901 gaagaatatt tgctaatttt aggggcgcga aagggtcagg ataaacatct acaaaccgga
     3961 cgtttagtgg aatttacatc aaaagattta actaattggg aattcaaagg tgatttttgg
     4021 gctccgggat tgtataccat gtttgaaatg ccagaacttt tcaagattgg tgactggtgg
     4081 tatcttgtct attcagaata ctcagatgga aataaaatac tctatcgaat gagtcaaagt
     4141 ttggaaggtc cttggattaa gcctaaagat gaagcttttg atggacgggc ttattatgca
     4201 ggtagaacag cttatgatgg aaaacgtcga gtcctatttg gatgggtacc aacaaaagat
     4261 caagaaaatg ataaaaacaa ttatttatgg ggaggtactt tggttcctca tgaagtctat
     4321 caaaaagagg atttttcact tggggtaaaa cccatagacg aagtgtggaa tgcattcata
     4381 aatttacatg atattgccga tgtagagata gaaacagcag acacaagaga agaagtaacg
     4441 cttgtcaaaa atagtggaac tttgtttaat ttagaaacaa caatttcatt tgatgagaat
     4501 cttagtgact tttcactgag attatataaa aaccttgaga cagatgaatc ttacgaatat
     4561 cgcttcaatt tagatgaggg gcgtcttaca tttgataaaa acccttgtta cccttggttt
     4621 caatatatgg ataaaggact tattagacct atatctttaa agcctaacca atcttataaa
     4681 ctaaaagtga ttgttgatga caatataatg acactttata ttgatggaat cgctcttaac
     4741 gctcgtgtac atggttgttt tggtcaagca attactatta cagcaacaaa tggatcaatg
     4801 actgcaataa ataccaagtt ttcaaatgaa ataaagaaat aataaacgtc aatcatgtgg
     4861 caggtctgaa aatcaaacat gacctgtcac atttaaaagg gaggataaat gaatggacta
     4921 caaaaaaatt gccgaagatg tattggaaaa cgttggagga aaagagaata tctcaagcgc
     4981 tacacactgc attactcgtt tacgtttaat tcttaaagac aaaagcaaag cgaatatgga
     5041 aatgcttgag gaaatagaag gtgcaaaagg tgttcttttc aatagtggac agttacaagt
     5101 aattttcgga acaggtataa ttgaaaaggt ttacaaagag ttcgttgaat tgacaggagc
     5161 taaggaagta tccgtttctg aaatgaaaga cgaaggagtc aataattcag gaaatagact
     5221 tcaacgagct tttaaagttt tctcggatat ctttattccg atcattccag cttttattgg
     5281 ggcagcaatg gttttaggaa ttcgttcgtt gattaccact cctggaatgt ttggaatgga
     5341 tggctcattc gctgatcaca atgtattcct aaatgattta tccaaatttc tgggtattat
     5401 tgcaaccaca tttgattatt taccagtttt agttatgtat tctgcaacaa aacgttttgg
     5461 cggaaaccct attttaggat tacttgtagg atttgttatg attcatccag gactagccaa
     5521 tcggaatgat ttcgtcatgg gaactataaa gcctgattat tggcatttat ttggattaag
     5581 cattccagca gttgcgtttc aagggggtgt ttttccagca attttgactt cttggttttt
     5641 agcaaaagtt gagaaaatta caaataaata ttctcctcaa gtacttgctt acattttagt
     5701 cccaactgtg acaattgttc ttgccaactt tgcactattt ttagtgtttg gtccattggg
     5761 gaattttatc ggtaatgttt taggtggaat cattgatttc ctatatatga agatgggtgc
     5821 ctttggtgct tttgtttttg caggcgcgtt acaaccatta gttgtaacag gaacacaaca
     5881 tgctattcaa ggtattgagg caaacttagt cgcaacaact ggattcaatt atatccaacc
     5941 tatttggtca gtttcaatta ttgcccaagg tggcggttgt attggtatgt atttactctt
     6001 taagaaaaaa tctaaagata gagatattgc gatttcaagc tttattccaa cactggtagg
     6061 tattactgaa ccggctatct ttgctgttaa tttgaaatat tcaatcgttc cttttgtttg
     6121 ctcgtttatt ggtgctggtt ttggtggagc atttatgaaa atttttgatg taaaagccat
     6181 tggacaagga ttgactgtat tgccaggtct gactattgct aatccagttc ttccttatat
     6241 atttggtaat ctcatagcat tcattttacc aatcattttt attctcgctt atgataaata
     6301 taaagtgttt atccctgaag ctaaacaccg taaatttgca gaaaaaactg actcaaaaaa
     6361 attgccagtt caagtaaagt gagatgagag catgttaaat ttttttagaa aaaatgaaat
     6421 aagcgttgta gcacctgcaa ccggtgtttt tgaaaaactg gagaaattaa gtgatccagt
     6481 cttttcaaaa ggaatgatgg gacaaggatt tgcaattgat gcaaaaaacg aaacgattgt
     6541 atctccaatc gatggttttg tttcaagtat ttttcctaca aaacatgcct taggaataaa
     6601 aacaaaatca ggaattgaga tacttgttca tgtaggaata gatacagttg agctaaatgg
     6661 tgagggattt gatattaaag ttcaagaagg acaaaaagtt aaggctgggg atattcttat
     6721 gaccattaat tttgtagttt ttgaaaagaa tcacaagact aaagatgtca ttgttgtttt
     6781 tccagactac caagacgaaa ttttcattcc gaaacttgaa gaagtaaaac aaggacaaga
     6841 attgtttgtt cttggctaat gataagtaat taataataga atcgaggaaa atgtatggtt
     6901 tttggggcta ttgaagctgg tggaacaaaa tttgtttgtg ccattggaga taaagatttg
     6961 aacatatttg aaaaagtcag ctttcccacg actacaccca aagaaacaat gaatttagtt
     7021 ttagattttt ttaatcatca taaagatgat ttagaaggta tcggagtggg atcgttcggt
     7081 cctattgata ttcattgtga atctaaaacg tatggcttca ttacatcaac gccaaaactt
     7141 gcttggcagg attttgattt tattggaagt ttaaaaaagc aacttaatat tccaactgct
     7201 tggacgactg atgtaaatgc ctcttgctat ggtgaatatg tttctggaaa tggcagaggc
     7261 ctttcaagtg ttgtttacta taccgtagga acgggcatag gaggtggcgc tatccaaaac
     7321 ggaaacttca ttgaaggttt tagtcatcca gaaatgggac atatgcttgt aaaacgacac
     7381 aataaagaca acttttctgg gacatgtcca ttccacggag actgtttaga gggcttagct
     7441 gctggtccgg caatcgaaag aagatcaggg aaaaagggac aagaagtgac tgaaaatgac
     7501 gttacttggg aaatagaagc ttattacatt gctcagtgtg catataatac aacattgatg
     7561 ttatcacctg atgtcattat atttggtggc ggagtaatga aacaaaaaca cttaaaaaag
     7621 aaagtacaag ataagtttat tgagttgatc aacggatatg tcgctattcc cgatgttgac
     7681 aattatatta tgacaccaaa attgttagat aattctggta ttgtaggttg tttagcttta
     7741 gcaagagacg ttaaagaaaa atcttaattt ggaaaaaaga tcgtctcgac tatttgtttg
     7801 gttgatgcga tctttttttc aattagtaat tgaagggacg cctaaaagtg aaaaaaaaaa
     7861 tgaaagatga agtattagag tatatcaaaa ataatttaaa gtattacgat ttttctgctc
     7921 aagatattgc aatgaaattt tgtattaaaa gaaatgtcgc ttcccactat ttgaatcagt
     7981 tattttcaga tggaaaatta ctaaagaatg attctgttag accagtaatg ttcaagtata
     8041 atcaacaaaa gccaaaagac tgcttttcta agtttattgg agcagacata tctttgaaat
     8101 ccactataga taaatgcaaa gctactgtaa tgtatccccc taatggcctc ccattaataa
     8161 taaaaggaaa cagtggcgtt ggaaaaagtt ttttagcaag tttgatctat cagtatgctc
     8221 ttgatagaaa agtaattcat aatgacgcaa aatttgttgt tgtgaattgt gcagattatg
     8281 caaataatcc agagctattg tcagcagttt tatttggata taaaaaagga gcctttactg
     8341 gggctgaaaa agatactgca gggatgctat cttctgctaa tggaggatac ctttttttag
     8401 atgaggttca caatctaagt gcagaaaacc aagagaaatt gtttcttctt atggattctc
     8461 aaaagtatcg taaacttggt gaaagtgtgc aatgggagta tgcaaatgtt cgccttatct
     8521 tagccacaac agaagataaa aatacctctt tgttagcaac ttttagaaga cgcatcccag
     8581 ctgaaatcac tctgccagat tacaataata gaagtacatc tgaaaaaatt caattattat
     8641 ttgaatttct taaagatgaa gcaattacga ttgattcaaa aatattttgt tcgattgaac
     8701 tttttactga tcttttatcg aaaacatttg aaggaaatgt tggagaattg aggaatgaaa
     8761 tcaagcttct ttgtgcagaa ggttatttag gaaataagag aaagtcttca atttatttag
     8821 gggacaaact agacagcggt ttttggatag atccagaaat tagtatagat ttaaaaaaaa
     8881 tgtttcttaa atcactttct gaaattgatt tgattgaact ttttcaaaat cacatttcaa
     8941 ttgaagaaag catgagtcaa ttaagagata gaatacttaa agagtgtgca ggttcttctg
     9001 agtataatta tttagaaagt gacgaattta tagcattaag aaactatgtt gttaataaaa
     9061 tatcaccaat tattgattcg acaggtctat gtttattaga tgaattcttg gcggatatca
     9121 gtctatttat aatgtttatt gatgtaattg aagaaacatc aagaaatttt ttgctgtcta
     9181 agttcagaaa atttcgtatg aaaaatgata aacaaaaatt attggcaaat gagatttgga
     9241 gtagtttaga agtcgaagaa agtaaacaag aacttttgtt gaaatggata ctttttcttg
     9301 taaagaagtt ctatgtgaaa ataccagaaa cccattgtct gatagttatg cacggtaaaa
     9361 taacagcttc tgcgatcgca agggaaacaa ataagttgct gaatacatat gtttatgagg
     9421 ctttcgatat gccaattgaa ggtgagacta gtgacttaat taacctaatc aacaattttt
     9481 gtaaaagtat agatacgacg aatggactca ttctgctagt tgatatggga tcattggaac
     9541 aaatgtatga aaaaattgaa tccaatgtaa taggtgattt ggtaatttta aacaatgttt
     9601 ctacagcttt agcgattgaa tgtgggattc aagtttgcca aaaaaagcca attagtcatt
     9661 tttaccagat ggattttgat tcgtttcaag ttaaagttca gtattataaa ggattatcac
     9721 aaaagaaaaa tgtaatagta tcttgtctct ctggtgaggg gatttctgaa aaagttaagg
     9781 atattttaaa acggtatctc gataatcaag tggagatttt gatttttgat tttaacgcat
     9841 taaagaaaat agcaaaggaa aaagatacta tgatttttaa gaatacaatt tgtgttcttt
     9901 caaccactga attttacatt caaggaatcg attgtttaaa tttagaaaat ctaattaatg
     9961 ggaatcaaac cttagaaaaa ttaaataagt atatggactc ggatagctgt gagttctgct
    10021 taaatgaatt agttaaactc tttactattg aaggcgctag tactaagctt cgctttttag
    10081 attccaaaaa agtattcaat gagatagaaa aagtactata tttgtatgaa aaatattacg
    10141 atgtcaaaat cccaagtttt ctgagaatta atctatttct acatctttct ggaatgattg
    10201 aacgaattat gattggtgat ggaataacaa atgaacattt gaaagaggga gcgcaactat
    10261 ttgatacttt tttaaatgta tcaaaagatt ttttttcaga aatcaaagat atttactgta
    10321 ttgaaatacc caaaggagag tatgagttaa tctatacaat ttttgagcag actattttct
    10381 aaatagcata ctagcgtcta aaaaaatgaa agggtgtcca aagtatattt agacgctcat
    10441 ttaattaata tttactgtat tttcccgctt tattggataa tgttagttgg cacagtaatt
    10501 gcttatataa aagtataaga agaaaggaga gaacaaaatg gcgttttggc aaacattttt
    10561 agttgtgttg tatggatttt tcattaactg ggataaggaa gggcccaaaa tcggaacctc
    10621 ccaacctgtc gttgcaggtt tcttgaccgg acttattatg ggagatattc aaactggact
    10681 ttatatcggt gggactttgc aattaatgac actaggtatt actagttttg gtggtgcatc
    10741 agtaccggat tatcaaacag cagcattgat cggtacttat ttagcaatct caactaacca
    10801 ggatgcttct ataggaataa ctttagcaat accggtagca atgttaattg tacaactaga
    10861 tgttctaaag tggtcaacaa acattttttt tcaacaaaag gcagaaaaat tcgcacaaaa
    10921 agaagattat caaaaattag agtggatgca atatttggca tcaattaata caagctttgt
    10981 atctggaatt cctgttttgt taactgtgat attaggccca acgttagtgg gaagtgcaat
    11041 caattatatt cctgaatggg ttaccggtgg tttaaaggta gcagggggac tattacctgc
    11101 tgttgggata ggaatgctac ttaaatattt acctacaaaa gattactttg cctatttgtt
    11161 agttggtttc gttttggcag tttatttcca aataccaatt ttagggattt cgttacttgg
    11221 cgctgcaatc gcactaatcg tatataaaaa tagatcaaat aatactcaag tagcaccagc
    11281 tgcaacagga ggttttgacg aagatgaata agaaactgac aaaaatcgac aaacaagtaa
    11341 ttttccggtg gattctcaat ggtgcaactt cattaaatta tgaaaaaatg gaagggttgg
    11401 gttatgcgta ttctatgctt ccttttatca aggacacata taaagatgat cctgaaggaa
    11461 tgaaacaatc cgttttagct cacttacaat tttttaacac tacgccgtat acagcgcctt
    11521 acattattgg tatgaatgtt ggaattgaaa aagaagaaaa aaataagtct ttggaagcag
    11581 taagtgctct gaaaacaggt ctaatgggtc ctttagcggg aattggagat agtgtatttg
    11641 tagttattcc atggactatt tttggagcaa ttgcagctaa tatggcattg gaaggtagtt
    11701 ttgttggaat tattttgtgg atactggcat caattttatt aaggattact gcttatcctt
    11761 tataccttgc tgggaaagaa tcgggtgcga gcctactaac tcaaattgaa acaaaattaa
    11821 agtctattac tgatgcagta tcaattttag ggttaatggt ggtcggtgca ctcattgcca
    11881 cagttgtaaa agtgaatgtt ggctttgaat ttaaacaagg cgacttaaca atgaccggaa
    11941 atgaaatctt tgatcaagtt atgcctggtt tattacctgc atgtgtagta gcaattgtgt
    12001 attatttgtt aggacgaaag gttaaaccag tttatgtaat tctattaatc ttagtagttt
    12061 ctattgcgtt aagagcgtta aatattttga agtaggtgtt gaatatggat gaaaaaatga
    12121 aaggtattat acatgttcgt attgatgatc gaatgattca tggacaagta gcaacacaat
    12181 ggacaaatca attgagtgct aacagaatta tggttattaa taatgaagta gccaatgatg
    12241 acgtaaaaaa agctgtagtt cgcctcgctg caccacctaa tgttagaact tcaataatta
    12301 caagggaagt agcagttaaa aatattcttt ctggtaaata tgaaggacaa aaagttctta
    12361 ttgttgcagt ttctccaatc gatgttaaat ttttgattga taacggttta ccaataacaa
    12421 gtgtaaacgt aggaaattta tcaagaagga atggaacaga acgcatccgt ccgacgataa
    12481 atattactga agaagagaga gaggctttta aagaactaat tagtgatgga gttgaagtga
    12541 cagtgataca aactcctaga gattcaaaag aatttttaaa ggattttatt tattaagaac
    12601 agaggatgag tggatggagt ttataattac atctcatgga aatattgcca ttggatttgt
    12661 agatacatta aaaatgtttt ttggcgataa tttaaattgt catgtggtaa cacttggaga
    12721 tgcaggcatt gaagaattcc gtgaaaacat tgaaaaaata gtttctcaag taaaaaatca
    12781 agaagttatg gtatttgctg acttgattgg tggaacacct ttcaacgaat ttgctaaaag
    12841 aagtgaagta tttcaaaatg ggtttcaact tcttggtggc atgaatttag gaattttgat
    12901 tgaggctgtc aatttgagat tacaaggaaa aaaaagcgat gatatcatcg aaaatctaag
    12961 aaatatgaat acaattgcgt gtcttgcaga tatgcaaaga catactaacg aagaggatga
    13021 gtgataaatg caaaaaattt tttacaagcc taaagatttt tggtttgggg attgcatgcc
    13081 tttttataaa gatggaactt tttatctatt tcatcaacga gatacgagaa atcctaaacc
    13141 tttaactgat ccttttgggt ggtcactagt tacaaccact gattttgtga aatttgaaga
    13201 atacggggaa gtaattcatc gtggtggcaa cgatgacatt gatcaataca tttacgctgg
    13261 aagtattgtc caaggaatca atgagtattt agctttgtat actggacata atcgcaaagc
    13321 taagttggat ggaaaaactt cagaagtgtt aatgattgca agttctaaag atttaattca
    13381 ttggaaaaag gatggcgaag cagcaaaact tgttcctcaa gaaggatatg ataagaatga
    13441 ttggcgcgat ccttttgcaa tttttgatca agatcatgga aaatacgttt tgatactagg
    13501 agctagaaaa ccagcaagta agaaaaagcc gacggggaga ttggtctatt ttgaatccga
    13561 agatttaaaa gattggaatt tcaaaggaga ctactgggct aataatgaat ttaacatgat
    13621 tgaaatgcct gatatatttg aaattgatgg aacgtggtat ttactttttt cagagtattg
    13681 tgaagataaa aagactaaat atcgagtcgg agcaggatta ttttcagatt ggaaatcacc
    13741 agtagacgaa gctttcgacg gaaaagccta ttatgcagca agaacagtcg gaactaatga
    13801 aactggccgt tttctaactg gctgggtggc aacaaaagaa ggggagaacg atcaaaataa
    13861 ctgggattgg ggcggtgcac ttgttccaca tcaaattatt caaaagacag acaaaactct
    13921 tggtgttaaa ctgcctgaaa aaataattga gaattttaaa gaaatcactc cattttcaag
    13981 ttttaagtta gagaaagaat ttggaaaaaa agaaagagta attgttccta aaatcgaaga
    14041 ttattttcta tttgaaacaa ctttgactat tacatcgtta acaaaggagt ttgctttaaa
    14101 attatttgta gatagtgata ctcaagaagg atacgaattt cgtatttcat tgattaataa
    14161 tcagcttact ttcggaaaaa ctccgaacta tccttggcct caaatgtttg ataaggggct
    14221 gcaacgacca ctatttttgg aaataggcaa agaatatgaa ctaaagttaa ttgttgatga
    14281 tacgatagct gttttatata ttgataatgt tgctttaagt actagaatgt atgataaagt
    14341 cggaacagca ttaagtatga ctgttacgga gggagcaatc aaggtagata aactagacta
    14401 ttcaataaat tatgaatgtg gattttaaaa caattgaagg gatgatgtaa gtgaaattaa
    14461 atggtttaca acatattgga attccaacaa aagattttgg agcttcaaaa atcttttatg
    14521 aatccttagg gtttagtctg attaatgaag aaatgaatgt gggaagtaaa gtcggctttt
    14581 tcgagttgaa tggaacgata atagaaatat gggaagataa aactactgag tgtagaggag
    14641 ccattgatca tctagcatta gatacggatg acatagatga ggtattcaat tcaattcaag
    14701 atttaggata tgaattatta gataaaaata ttatggaatt accgttctgg gaaaatggaa
    14761 tccgttactt taatttctac ggaccaaatc atgagattat agagataagc caaatgaaga
    14821 aataaataga taacgaagtg ctctaagttg gtatgaacga aaagtacttc attttttggt
    14881 atcagcattt caatagttta atgagaattt actatttcaa attaattcaa agtgacagta
    14941 ttttttactt atttggtttc aaattttgta atccttccta tttattggaa tacactccac
    15001 attttgtata caagtcatat catgttttag caaagctctt accaagcaaa gttataatga
    15061 tgttttccaa agtttgatct ttcgatggag gctaacacat attttttgca ggacagccaa
    15121 aattagatgt atagaataga gtctgtgagg cgtatagaca gtttattgtc tgtatgcctt
    15181 tttttatttt gaaaaatttt tagtattttt cgaaaaatgc tcactcctag
//
LOCUS       DACDXO010000062        14964 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E23605_2017
            SAMN12639989-rid9881463.denovo.063, whole genome shotgun sequence.
ACCESSION   DACDXO010000062 DACDXO010000000
VERSION     DACDXO010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639989
            Sequence Read Archive: SRR10023389
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14964)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14964)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741914.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:42:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,972
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 33 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 17 of 73
            Pseudo Genes (multiple problems)  :: 10 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14964
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639989-rid9881463.denovo.063"
                     /isolate="E23605_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(445..1173)
                     /locus_tag="IWM35_002173"
     CDS             complement(445..1173)
                     /locus_tag="IWM35_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9920013.1"
                     /translation="MLRIAIVDDDCTIVEGIRHYLLKKVPDIIIDHCYTDGDEAFKNL
                     QNQRFDLLITDITMGKISGLELIESLQQTSNRYFTSIVISAHQNFQYAKKGIDLSIKS
                     YITKPIDFPYLVKLVQEEKMRKSAANQNSLTDDSFNESIQQVIDYVQNNYSEKLTLKD
                     VATQFHYHPAYLGRAFKKETGYSFHDYLLDFRIKRASALLKDTFLSIDEISQKVGIDH
                     VSYFSVLFKKTTGHSPSNYRKKLL"
     gene            complement(1151..2725)
                     /locus_tag="IWM35_002174"
     CDS             complement(1151..2725)
                     /locus_tag="IWM35_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP9920014.1"
                     /translation="MKNDLSKVIMRFTYILIFFITLLACFTLFFSYINVIEKEKNQNL
                     QNIQQITHYMETYNRILSGIVTEIYQNYDFQENLNDYYLSSPAEYMEDILSKDRSPTY
                     LVDNLKKLVQDDEVKNISISLDYEKYLSAYAPTKNEKISFIRSLSFISEDMPDATLIL
                     DVSINALKKSLFPTHYNYSLSNGIDSVNLSEDISPSKWKKTQQIAIGNHTLQVYENPN
                     YSSQTLLGLAIMIFLLMSLFILAIHQLLNRSFRNYIQQYQEIITSLNHSSTGDYKSIN
                     IENKVGDLKEIAMHINQFYLDNATYQKTLYENNWLKQEAKYAALQYQIEPHFLFNNLE
                     FIRMTAILNDDEEISDSIYLLSKLYRNNTYQPDIIDLKEELINIDLSLQLHQRRYRHN
                     FDYQLIDHIDQLMLPKLSLQPFVENYIKHGFDSSKKENRLTVYATETTNHYVLKIVEN
                     GKGMDQDKLEHIQQKLNDDIPYSENIGISNSFRRIKHLYPGDTRFVIESQLHVGTTIT
                     IKIQKGGNMHVENCNC"
     gene            2954..3892
                     /locus_tag="IWM35_002175"
     CDS             2954..3892
                     /locus_tag="IWM35_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP9920015.1"
                     /translation="MKLKKGLLGNLKKNRALFIMLLPGALWLFIFCYIPMFGVVIAFK
                     DFRISSRGFFTSLFNSPWVGWENFKYLFASQDAWIITRNTVVYNVVGILLSTFCTVSL
                     AIILSQMANKRLLKKMQTFMIFPNFLSWVVISYFVYAFLSTDKGLINILLSNFGVEPI
                     NWYSEPKYWPFIIVFTSLWKGVGYGSIIYYASIMGIDTSYYEAALVDGASKWQQIRNV
                     VLPHLRSIIVIMLILSVGSIFRADFGLFYQLPRNSGALYDVTNVLDTYVYRGLMNSGD
                     IGMSTAAGLYQSSVGFVLILITNKIVNKLDPSSALF"
     gene            3904..4815
                     /locus_tag="IWM35_002176"
     CDS             3904..4815
                     /locus_tag="IWM35_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP9920016.1"
                     /translation="MISKLKRQMYFSLPVERFFTILLILFTLSCIFPFIFVIVISLTD
                     EKALQLNGYELLPAQWSTDAYQYLIQDGGQLLRSLGVTIMITVIGTLITVFMTGTYAY
                     VLSRASFPYRKFFTFYLFFTMLFAGGMVPSYLVMTKMLGLKNTIWALILPLAFSPYNV
                     IILRTFFKKSIPESIIESAKMDGCSEFRVFFQIVLPLAIPGVATIGLFSSLGYWNDWF
                     NALLYIDTNKLIPLQYLLMKIQNSMEFLANNNDITLAQQQAIQNSLPQESTRMAMVVV
                     ATLPIAIVYPFFQKYFVQGLTIGGVKE"
     gene            4839..6311
                     /locus_tag="IWM35_002177"
     CDS             4839..6311
                     /locus_tag="IWM35_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP9920017.1"
                     /translation="MKKNMQKYLIGGASVLTLGLLGACGSSNGEANNETGTTKVTWYQ
                     VGEEPKDFDEVMTKVNEKLKEEINVELDMRLIPDGDYQQKLGVMINSGEEYDICFVNG
                     TDYVNYGNKGAFISLNDHLDKELKDYAAELNEGFIEGGAINGETYGLPVNNDIATTEY
                     WTFNKDLVDQYQLDVENVEGVETLAPVLEKFAEKNTDTNITPIGMTRTSVCIPYDNII
                     GGGVPLAVAFEGDTQTVVNTYETPEMMTYLKAMHEFYKKGYILKDAATVTTDPFRIDS
                     KNWFLGRNSSGYQPLAQKKLEALAGHGLILKPAGKAFKTTGFARVAMHAVSASSKHPE
                     AALKLLNYVNTNQEVADLMVYGIEGKHYDRNEDGSITLTNSDDYYQGAWALVNTYLVS
                     KTSPITEESKKLEEESKKFQNEAPMSPILGFSFDASDFRTEIASVQNIVSQYEVLLSS
                     GTVDPEQYVPELLEKLEKAGLDKIQASAQEQYDEWRKSNK"
     gene            6362..6973
                     /locus_tag="IWM35_002178"
     CDS             6362..6973
                     /locus_tag="IWM35_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAP9920018.1"
                     /translation="MNENKWTKGFNWVFQLLLLNFTYFILICLGFGILGFFPATIALI
                     AGVVNRKKYGLQESVKFMSKIYKKNFVRYNLYCWSATLLLAGNITTIWLCWQQSAPLF
                     YLLSTLLSVITLFLVFAMLNWVVIKSLGLWMMGDRLIDYLLFTATMLPTVVMQGLVLG
                     IYFRILMILPVIFLFGGTAVTVILISKLTERALAKLPCQIQSH"
     gene            7003..8916
                     /locus_tag="IWM35_002179"
     CDS             7003..8916
                     /locus_tag="IWM35_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HAP9920019.1"
                     /translation="MKLTDKQVANIAFIQQLEIPLTQKDEEQIDLIFTDTKANVRQIK
                     ITKNEQQRICLSYSHEDLLYRGLFICLPRFLAQGGEMPYIEETSIIETSYSLDASRNG
                     VMNKETLKKFISYLALMGYNTLYIYTEDTYEVEDLPYFGYLRGAYTKEEVNEIVEFAS
                     GFGIEVVPSIQTLAHLTQFLKWYAQQELMDDQNTLLIGEEKVYAAIEKMIIACKEMYQ
                     TERIHLGMDEAYQAGLGRYLSLYGYCDRTTLMIQHMDRVLSIVDKYKMKPMIWSDFIY
                     KLLDETGKDRYYNPDFKLKALAAKGLPAGLTYVHWDYGSDEVSSYEKVIDNHLKFCDI
                     DHYVLASGAHIWGRLAPNHGKTEAIVSAAVKASKNKGVRSVMLTTWGDEGQETEHWHS
                     LIGALKLADYVYSEEEWNDFEEIYDRLLGTGSYQLMSELRYFDEVDTITNNNLQMASV
                     SKLLLWQDPLVGIYDFHVAAHNKIATRTIGTYYHELAQKLDMNKNWNPKQPILTTIRQ
                     RYILLAEILAQKAELGIYLQHCCLVEDLAGLMELANRCEALANRVNELASIHEIIWYQ
                     TYKTYGWEVLEQRYAALAHRLKTVARRIKNHLQGQDALEELLEERLPFCQYQVPLEIS
                     GFNYRQSSVSGYN"
     gene            9044..12025
                     /locus_tag="IWM35_002180"
     CDS             9044..12025
                     /locus_tag="IWM35_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 42"
                     /protein_id="HAP9920020.1"
                     /translation="MLSDYYQNLSVISVNTEPLRNYYIPYASKEEAKQELEREKTSQF
                     IDLNGTWDFQYYPSVQQLPENFFQEAFTESYDRISVPGVWQTEGFDLPQYINVSYPIP
                     FDPPYVPNDTPCGVYRRQFQIEQTDARFYLNLEGADSNCFVWINRNFVGYDQVSHSTS
                     EFDVTKYLQVGVNEIQILVMKWCDGTYFEDQDKWRMSGLFRPVYLLQRPTMHLTSYQL
                     RTLTDPKKGSGTLMVSFQRFTPIEVSLPISYELFHPDGRLIAKGECYSEQQINLDVED
                     CLFWTAETPKLYTLLLTCNGEYFKEKVGFRALTIKNRKLLLNGTPIKLYGVNYHDVNQ
                     QGTPVMTREQLRADLLLMKKLNVNTVRTAHYPKSPDFYELADEIGLYVINEADLECHG
                     VVNLYDTEANYQLIANDPTFEKPIMDRVIRSILPYQNRSSIIMWSMGNESGYGVNFEK
                     AQALARKLDDSRLIHNERSIEPVIDQVNDFSNLDVISQMYPPLEDVHRHCQRSDTEKP
                     FFMCEYSHAMGNGPGDLKTYFDLIQQYDSFLGGCVWEWTDHAINRGTLETPSYLYGGD
                     FGEKYHDGNFCVDGLLTPDRQLTVGALEYRNIHSPIQLIAVDEELQEVTLSNITAFTA
                     GSAFNLSLEWYIDGKKTVYEVMNPFILEPGKSCSLPITVPTTIHGAHTVAVSLSVYKN
                     DELYGQQQLFLKKEKMRLPRTAHHNDAPQISVKGTELIIKGESFIYIYQLAKAAFTSV
                     KINQKELLERPTQFSIWRAPTDNDRNLVNKWKAAGYAEMSLEIRKKDWKFVDDHLLIE
                     TSYRGVIPGLQPIFTMHIQLKINGAGVINQKVSVEKNPIFPDFPRFGMDWPLNDTFSK
                     VSYCGYGPYESYPDKHYAAMFGIYEMEIEEFDQPYIKPQEYGNRWQCDWVSIKNHQGS
                     TMTVYGNSNFSALPYSQAQLTEKSHHYELIKENRTWFNLSYKQNGIGSHSCGPYLEDR
                     FRFMEDTFDWNIVITFKGENR"
     gene            12022..13683
                     /locus_tag="IWM35_002181"
     CDS             12022..13683
                     /locus_tag="IWM35_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hyaluronate lyase"
                     /protein_id="HAP9920021.1"
                     /translation="MKETKINQVKKKLSRYQEVVYRNHCLKWSKNKLNVRFNGLFYRF
                     EQLEEVWKPVTNFQSCPVQLINDPKETYDLCITHLYNQHLKNDGFYLKVTNDNQLTIE
                     SANLRGFQYAMEALLESFFSVGDQLLLPELILKHEPSVKIRGIIEGFYGIPWTHEMRL
                     SLFSYMKDNQLNTFMYAPKDDELLRKKWRELYDAQELDKFKELLSAAEASNIDFWYLI
                     SPGNDIDITCEEDIQVLLKKLEQLIELGVFQFGLLMDDIDYQLKGAAKRRFREPAFAH
                     AYLVNRVEEYLSTRLSNHELVICPTEYDNRHGSRYLATLSQEIPEQLPFFWTGPSTLA
                     ASISTEELQEMASVYQRPMLIWDNIPVNDYLEDKELLFMSPYENRTPNLSKERYQVTG
                     VVSNPMAQLEASKFTINSMANYLWNCERFDPLETWTSVVEKVVGSKLQPAYLTLTNAF
                     PNHYTSSLLSDEELLLAKDPEWVTKEMAALVQAVKWIQQEQHTSRLRTELEPWLQAIT
                     ESWTFWQEWQLKKDPKEAEEWLAKKKTHRIGRDLVTAHLEQIIKS"
     gene            13723..14655
                     /locus_tag="IWM35_002182"
     CDS             13723..14655
                     /locus_tag="IWM35_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HAP9920022.1"
                     /translation="MVKRLISANYSDVKKMTSKELKQSIKASEGRVIVCETVANTLPQ
                     SGEITNAEVASAFGADLILLNGFDCYRPAVFGLPNVTIEKVFDLQQTIENPIPKLKEL
                     VGRPVGINLEPIAPKTNMMSEKVEISQGRASSVETIQKAEELGVDFICFTGNPGTGVT
                     NLEITKSVKMAKKYFSGLIIAGKMHSAGSEEPVVNKEAIISYAEAGADILLVPSVGSI
                     PGFLEQDLIEAVRLAKKYDLLTMSAIGTSQESARPETLRQLAIMNKSCGVDLQHIGDA
                     GYGGLAPAENIFETSVAIRGMRHTINCVSRSINR"
BASE COUNT         4863 a         2366 c         3005 g         4730 t
ORIGIN      
        1 gcaactttta taactttcgg gcacggatct atttacaaca aggtctgatc tttgaaaact
       61 aaaaaaatat gcggcgcaca gaacgcgccg catatcggac taagttttat tcaaatttgt
      121 cttcaccaac acgatttgac gaagagccta tttaaataac tacatccatt tattctatct
      181 tctaaaatag cctttttcat ttcagttaaa tagttcagcg ccatttttgc actcgacttg
      241 catagtagtt ttttccatat tcctcaacct cagcgggggt tgaagacata aggaaaatcc
      301 tatgcgttat gctcaactgg atctgattaa atattctaat gtatttttct ctaccatcat
      361 taaatggtta atagccaaaa atcaccactt ttcaatggct ccttgttgta taagtataac
      421 tagaagatga acacgtctga gaaattatag aagcttttta cggtaattac tggggctgtg
      481 tccagtagtt tttttgaaga gaacagaaaa atagctcacg tgatctattc ctactttttg
      541 actaatttca tcaatagata agaatgtgtc tttcagtaga gcacttgctc ttttgattcg
      601 aaagtctagc aagtaatcat gaaaggaata acctgtttct tttttgaagg cccgtcctaa
      661 ataagcggga tggtaatgga attgtgtagc tacatctttg agcgtaagct tttcactata
      721 attgttttga acgtaatcaa tcacttgttg aattgactca ttaaaagaat catcagttag
      781 agagttttga ttagctgctg atttacgcat tttttcttct tgtactaact taactaaata
      841 aggaaaatca atgggtttcg ttatgtaact tttaatagat aaatcaatcc ccttttttgc
      901 atactgaaaa ttttggtggg cactaatgac aatactagta aagtagcgat tggatgtttg
      961 ttgcaaagat tcgattaatt cgagtccaga aattttcccc atcgtaatat ctgtgataag
     1021 taaatcaaag cgctggtttt gcaggttttt aaatgcttcg tctccgtctg tgtagcagtg
     1081 atcgataatg atgtcgggga ctttcttcaa aagataatgc cttatgcctt ctacaattgt
     1141 acaatcatcg tcaacaattg caattctcaa catgcatatt cccccctttt tgtattttga
     1201 tagtaatcgt tgttccgacg tgtagttggc tttctataac aaaacgtgta tctccaggat
     1261 agagatgctt tatacgtcga aaagaatttg agatgccgat attctcacta taaggaatat
     1321 catcattaag tttttgttga atatgctcca gcttgtcttg gtccattcct ttcccatttt
     1381 caacgatttt taacacataa tggttggttg tttcagttgc atagacagtc aaacgatttt
     1441 ccttttttga tgaatcgaag ccgtgtttaa tataattttc aacaaatggc tgtaaagaaa
     1501 gttttggtaa cattaattga tcaatatgat caatcagctg atagtcaaag ttatgtcgat
     1561 aacgacgttg atgtagttgc aaggagaggt cgatattaat taattcctct ttcaaatcaa
     1621 ttatgtcggg ctgatatgta ttattgcgat acaacttgct caataaatat atactgtcgc
     1681 tgatttcttc gtcatcattc aatatggcag tcattcgaat aaattccaaa ttattaaata
     1741 gaaaatgagg ctcgatttga tattgaagtg ctgcatactt tgcttcttgc ttcaaccaat
     1801 tattttcata aagtgttttt tgatatgtag cattatctaa ataaaactga ttgatatgca
     1861 tagcgatttc tttcaaatca ccgactttgt tttcgatatt gattgacttg tagtctcctg
     1921 tagatgaatg atttaaggaa gtaatgattt cctgatactg ctggatatag ttacgaaaac
     1981 ttctatttaa taattgatga atagcaagaa taaacaaact cattaataag aaaatcatga
     2041 ttgccagacc taataaggtt tgtgacgaat aatttggatt ttcatatact tgtaatgtgt
     2101 ggttaccgat agctatctgt tgcgtttttt tccatttaga aggcgagatg tcttcagata
     2161 aattgacgga atcaatacca ttagacaaag aataattgta atgtgttgga aagagactct
     2221 tttttagcgc gttgatcgaa acgtctagaa tcaatgtagc atcgggcatg tcctcactaa
     2281 tgaaacttaa tgaacgaata aaagaaattt tttcgttttt tgtaggtgcg taggcactta
     2341 agtatttttc gtaatctaat gagatagata tatttttaac ttcatcatct tgcacaagtt
     2401 tttttaagtt atctactaaa taggtagggg agcgatcctt tgacaaaata tcttccatgt
     2461 attcagcagg tgatgagagg tagtagtcat taaggttttc ttggaaatca taattttgat
     2521 agatctctgt cacgataccg gagagaatac gattatatgt ttccatatag tgggtgattt
     2581 gctgaatgtt ttgcaggttt tgattttttt ctttttcaat cacattgata taggaaaaga
     2641 acaatgtgaa acaggcaagt aaagtaataa aaaaaatcaa tatgtatgta aaacgcataa
     2701 taacttttga taaatcgttt ttcattcaaa gtcctcattt tgataatctc taattttatg
     2761 aatcattata gcacatatta caagtgatgt aaacgctttg caaacagggc tacttatgat
     2821 tgtgaataca gtttccgttt aaaaactatt ctattgtaga ggtttaataa actaaaaata
     2881 atgtataaaa aattaatgta agcgattaac caagtgggta taatcaagga aacgctgaga
     2941 gaggagagac aaaatgaagc taaagaaagg gttattagga aatctaaaaa aaaaccgagc
     3001 attgtttatt atgcttttac caggtgcgtt atggttattt atattttgct atataccaat
     3061 gtttggagtt gtaatcgctt ttaaagattt cagaatttca tcaaggggat tctttacaag
     3121 tttgttcaac agtccttggg ttggctggga aaatttcaaa tatctctttg cttcgcaaga
     3181 tgcttggatc attactcgca atacggttgt ttataacgtt gtaggtattt tgctaagtac
     3241 tttttgtaca gtgtcattag caatcattct gagccaaatg gcgaacaaac ggttattgaa
     3301 aaagatgcag acatttatga tttttcctaa ttttttatca tgggtagtca tcagttactt
     3361 tgtttacgca tttttgagta cggataaagg attaattaat attttactaa gcaattttgg
     3421 tgtggaaccc ataaattggt atagtgaacc gaagtactgg ccttttatta ttgtttttac
     3481 gagtttatgg aaaggcgtag gctatggatc catcatttat tacgcgtcaa ttatggggat
     3541 cgatactagt tattatgaag ctgctttagt tgatggtgcc agtaaatggc aacagataag
     3601 gaatgtagtc ttgccacatt taagatcgat tattgtcatt atgttgattt tgtctgttgg
     3661 tagtattttt cgagctgatt ttggattgtt ttatcaattg ccaagaaatt cgggggctct
     3721 ttatgatgtg acgaatgtat tagatactta tgtctaccgt ggattaatga atagcggaga
     3781 tatcggaatg tctacggcag cgggattata tcagtcatca gttggctttg tcttgatttt
     3841 gattactaat aagatagtca acaagttaga tcctagttca gcactatttt aggaggaatc
     3901 aaaatgatat caaaattaaa acggcagatg tacttttcgc tgccagtcga acggtttttc
     3961 acaatattac taatcttatt tactcttagc tgtatttttc cctttatttt cgtaatcgtc
     4021 atttcattaa cagatgaaaa agcattacaa ctcaacggtt acgaattatt gccggctcaa
     4081 tggagcactg atgcatatca atacttaata caagatggcg gacagttact tcggtcatta
     4141 ggcgttacaa ttatgattac agtgattgga acgttgatta ctgtttttat gacgggaacc
     4201 tatgcttatg ttttatcaag agcctctttc ccttaccgga agttctttac tttttatctt
     4261 ttttttacca tgttatttgc gggagggatg gttccttctt acttagtgat gacgaagatg
     4321 cttggcttaa aaaatacaat ttgggctttg attctacctc ttgcttttag cccatacaat
     4381 gtcattattt taaggacatt ctttaagaag tcgattccag aatcgattat cgaatccgct
     4441 aaaatggatg gctgtagtga gtttcgagtt tttttccaaa tcgttttgcc tttagcgatc
     4501 ccaggggttg caacaatagg tttattttca agcttaggtt attggaatga ctggttcaac
     4561 gcattgctgt atattgacac caacaaattg attccattgc aatatttgtt aatgaagatt
     4621 caaaattcaa tggaattctt agcaaataat aatgacatta cattagcgca gcaacaagca
     4681 attcaaaata gccttcctca agaatcaaca cgaatggcaa tggtcgtagt agctacttta
     4741 ccgattgcaa ttgtttatcc gttcttccaa aaatactttg ttcaaggatt gacgatcggt
     4801 ggagtgaaag aataaaaaag aataggagat gttaaaagat gaaaaagaac atgcagaaat
     4861 atttgattgg tggtgcttct gtactaaccc ttggcctatt aggtgcttgt ggtagcagta
     4921 atggcgaagc aaataatgaa acaggaacca caaaagttac ttggtatcag gttggagaag
     4981 aacccaaaga ttttgatgag gtcatgacaa aagtcaacga aaaattaaag gaagaaatca
     5041 atgtagagtt ggatatgcgg ctgattccgg acggggatta tcagcaaaag cttggtgtga
     5101 tgatcaattc aggagaagaa tatgatattt gtttcgtcaa tggcacagat tatgtgaatt
     5161 atgggaacaa aggtgctttc attagtctca acgatcattt ggataaagag ttgaaagatt
     5221 atgcagctga attgaatgaa ggatttattg aaggtggggc aattaatggc gaaacttatg
     5281 gattgccagt caataacgat attgccacaa cggaatattg gacctttaat aaagatttgg
     5341 tagatcaata ccaattggat gtagagaatg ttgaaggtgt agaaacattg gctccagtat
     5401 tagaaaagtt tgcagaaaaa aatactgata cgaatattac gccaatcggt atgacccgta
     5461 ccagtgtctg tattccttat gacaatatta tcggtggtgg tgtaccgcta gcggttgcat
     5521 ttgaaggcga tacacaaaca gttgttaata cttatgagac tccagaaatg atgacttatt
     5581 tgaaggctat gcatgagttt tataaaaaag gatatatttt gaaagatgca gccactgtta
     5641 cgacagatcc atttcgaatt gatagtaaaa actggttttt aggtcgtaac tcaagtggtt
     5701 atcaaccatt agcacaaaaa aaattagaag ctctagcggg acatggatta attttaaaac
     5761 cagctggaaa agcttttaaa acgactggat ttgcaagagt ggcaatgcat gcggtatctg
     5821 cttcaagtaa acatccggaa gcagccttga aattgttgaa ttatgtgaat accaatcaag
     5881 aagtagcgga tttaatggtt tatggaattg aaggaaaaca ctatgatcgc aatgaagatg
     5941 gttcgattac tttaacaaac tctgatgatt attatcaagg cgcttgggca ttggtaaata
     6001 cttatctagt ttcaaaaact tcaccgatta cagaagaaag taaaaaacta gaagaagaaa
     6061 gtaaaaagtt tcaaaatgaa gcaccgatgt ctcctatttt aggtttctct tttgacgctt
     6121 ctgattttcg aacagaaatt gcaagtgtac aaaatatcgt gtcacaatat gaagtacttt
     6181 taagctcggg aacagttgac cctgaacaat atgtaccaga gttgttagaa aaactagaaa
     6241 aagctggatt ggataaaatt caggcatctg ctcaagaaca atacgatgaa tggcgaaaat
     6301 ctaacaaata attttttact aaatgaaaag aatagactaa gcagttagtg gggggattca
     6361 aatgaacgaa aacaaatgga ccaaaggatt taattgggtt tttcaattgt tgttactaaa
     6421 ctttacttat tttatactaa tttgtttagg atttggaatt ttaggttttt tccctgctac
     6481 gatagctttg attgctggcg ttgtgaatcg aaaaaaatat ggcttgcaag agtctgtgaa
     6541 gttcatgagt aaaatctata agaaaaattt tgttagatat aatctatatt gctggtcagc
     6601 aactttgttg ttggccggca atattactac gatctggctt tgttggcagc aaagcgcacc
     6661 attgttttat ttattaagta cattattatc ggtaattaca ctgttcctag tctttgcaat
     6721 gttgaattgg gtcgtgatca aaagtcttgg gttgtggatg atgggagacc gactgattga
     6781 ctatttgctt ttcacagcaa cgatgttgcc aacggtagtc atgcaaggct tggtgcttgg
     6841 aatttatttc cgaatattga tgattttgcc tgtgattttt ctgtttggcg gaacagctgt
     6901 aacggtaata ttgattagta aattaactga aagagcgtta gcaaagttgc cttgtcagat
     6961 acaatcgcac taattttttc acaaaaagga gtatttcttt ctatgaagtt aactgataaa
     7021 caagtagcta atatagcctt tatccagcaa cttgagattc cattgacaca aaaagatgaa
     7081 gaacaaatag acttgatttt tactgacaca aaggctaatg tgcgacaaat caagattact
     7141 aaaaatgagc agcaacgaat ttgcttgtct tattcacatg aagacttgct gtatcgagga
     7201 ttgtttatct gccttccacg ttttttagct caaggaggag agatgccata cattgaagaa
     7261 acctcgatca ttgagaccag ttattcatta gatgcttctc gtaatggcgt gatgaataaa
     7321 gagactctaa aaaaattcat ttcttactta gcgttgatgg ggtacaacac attgtatatt
     7381 tacacagaag atacctatga agtagaagac cttccatatt ttggttacct tcgcggagcg
     7441 tatacaaaag aagaagtgaa tgaaattgtt gaatttgctt ctggttttgg catagaagta
     7501 gttccatcga tccagaccct tgctcacttg acccaatttt tgaagtggta tgctcaacaa
     7561 gaactgatgg atgatcaaaa tactctactg atcggtgaag aaaaggttta tgcggcaatc
     7621 gaaaagatga ttattgcctg caaagaaatg tatcagacag agcgtattca tttgggaatg
     7681 gatgaagcct atcaagcagg tctgggacga tatttaagcc tgtatgggta ttgtgatcgg
     7741 acaactttaa tgattcaaca tatggaccgt gtgttatcaa ttgttgataa atataagatg
     7801 aaaccaatga tttggagtga tttcatttat aagctattag atgaaaccgg taaagatagg
     7861 tattacaatc ctgattttaa actcaaagca ttagcagcta aaggattacc agcaggatta
     7921 acctatgttc attgggacta tggttcagat gaagtttcct cctatgagaa agtaattgat
     7981 aaccatttga aattttgtga cattgatcat tacgttttag ctagtggtgc ccatatctgg
     8041 gggagactag ccccgaatca tgggaaaaca gaagcaattg tttctgcagc tgtaaaagct
     8101 tctaaaaata aaggtgtacg ttctgtgatg ttaactactt ggggagatga agggcaggaa
     8161 acagagcatt ggcattcctt aattggggca ttaaaattag cagattatgt ctattctgaa
     8221 gaagagtgga atgattttga ggaaatctac gatcgtttat taggaacagg tagttatcaa
     8281 ttaatgtccg aattacgata tttcgatgaa gtggatacca ttacaaataa caacttacaa
     8341 atggcttccg tgagtaaact gctactttgg caagatccgt tagttggtat atatgacttt
     8401 catgtagccg ctcataacaa aattgccact agaacaattg gtacttatta tcatgagtta
     8461 gctcagaaat tagatatgaa taaaaattgg aatccaaaac aaccaatttt aacaactatt
     8521 cgtcaacgat atatactttt agctgaaatt ttagcacaaa aggcagaact aggcatctat
     8581 ttacaacact gctgtctggt agaagacctt gcaggattaa tggaattagc gaatcggtgt
     8641 gaagcactag ccaatcgagt aaatgaattg gcatctattc atgaaataat ttggtatcaa
     8701 acatacaaaa cctatggttg ggaagtatta gaacaacgat atgctgcatt agctcatcgt
     8761 ctgaaaaccg ttgcgcgtcg tattaaaaat catcttcagg ggcaagatgc cctagaggag
     8821 cttttggaag aacgattacc gttttgtcaa tatcaagtgc cgcttgaaat ttctggcttt
     8881 aattatcggc aaagctcggt gtcaggctac aattaacgaa ggaatcttcc cgttgaacgg
     8941 accacttgct catctaatgg gaaagtggtt cgttttctat tcataagtag cttaccacta
     9001 actcttttat taagaagaga aaaaaagtag gaagtgagaa aatatgttat cggactatta
     9061 tcaaaatctg tcagttataa gtgtcaatac agaaccatta cgtaattatt atattcctta
     9121 tgcaagtaaa gaagaagcaa aacaagagtt agaaagggaa aaaacgagcc agtttatcga
     9181 tctcaatggt acatgggatt ttcagtatta tccttctgtc caacagttac cggaaaattt
     9241 ttttcaagag gcctttactg aaagctatga tagaatttct gttcctggag tttggcaaac
     9301 agagggattt gacttacctc agtatatcaa tgtatcttat ccaattcctt ttgatccgcc
     9361 ttatgtacct aatgataccc cttgtggtgt atatcggcgt cagtttcaaa ttgagcaaac
     9421 agatgcacgc ttttacttaa atctagaagg agcagatagt aattgttttg tttggataaa
     9481 tagaaacttt gtaggctatg accaagtgag tcatagtaca tctgaatttg atgtgaccaa
     9541 gtatttacag gttggggtta atgaaattca aattctagtg atgaagtggt gtgatggtac
     9601 ctactttgaa gatcaggata agtggcgaat gagcggtcta tttcgtccag tgtatcttct
     9661 tcaacgacca actatgcatc tgacaagtta tcaactacgg acgttgactg atcctaaaaa
     9721 aggaagcgga acattaatgg tttcttttca acgatttact cctattgaag tatcgttacc
     9781 tatttcttac gaattattcc atcctgatgg acgtctcata gcgaaagggg agtgttactc
     9841 ggaacaacaa ataaatttgg atgttgaaga ttgccttttt tggacagcag aaacgcctaa
     9901 attatataca ttacttttaa catgtaatgg tgaatatttt aaagaaaaag ttggctttcg
     9961 agcactgacg atcaagaatc gtaagttatt gctgaacgga acacctatta aattatatgg
    10021 agtcaactat catgacgtga atcagcaagg cacgccagtg atgacccgag agcagttacg
    10081 tgcagatttg ttattaatga aaaaattaaa tgtgaatact gtacgaacgg cacattaccc
    10141 taaatcacca gatttttatg aattggcgga tgagattggt ctgtatgtaa tcaatgaagc
    10201 agatttggag tgtcatggtg tggttaatct atatgatact gaagcgaatt atcaactaat
    10261 tgctaatgat ccgacattcg aaaagccgat tatggaccga gtgatccgtt ctattcttcc
    10321 ttatcaaaat cgaagctcta tcattatgtg gtctatgggg aatgaatctg gttatggagt
    10381 aaattttgaa aaagctcaag ctttagcacg gaaattggat gattcacggt tgattcataa
    10441 tgaacgctcg attgaaccag ttattgacca agtgaatgat tttagtaact tggatgttat
    10501 tagtcaaatg tatcccccac tggaagatgt tcatcgtcat tgtcaaagaa gtgatactga
    10561 aaaacctttt tttatgtgtg aatatagcca cgctatgggc aacggtcctg gagatcttaa
    10621 aacgtacttc gatttgattc aacagtatga ttcctttctt ggcggttgtg tctgggagtg
    10681 gactgatcat gcaatcaata gaggaacttt agaaacgcca tcttatttat acggtggtga
    10741 ttttggtgaa aaatatcacg acggcaattt ttgcgtagat ggtttattga ccccagatcg
    10801 gcaattaaca gtaggtgcat tggagtaccg taatattcat tcgcctattc aactgattgc
    10861 tgtagatgag gagctgcaag aagtaacctt gagcaatata actgctttta cagccggttc
    10921 agcatttaac ctttctcttg aatggtatat agatggaaaa aaaacagttt atgaagtaat
    10981 gaatcctttc atacttgagc ctggcaaaag ttgttcattg cccatcacag tacctacaac
    11041 gattcatggt gctcatacgg ttgctgtttc gctatctgtt tataaaaatg atgagcttta
    11101 tggacagcaa cagctctttc ttaaaaaaga aaaaatgaga ttgccaagaa ctgctcatca
    11161 taatgatgca ccacaaattt cagtaaaagg cacagagctg ataatcaaag gagaatcctt
    11221 tatttatata tatcaattag caaaagctgc gttcacttct gtaaagatca atcaaaagga
    11281 acttttggaa cgtcctactc aattttcaat ttggcgtgca ccaacggata atgatcgtaa
    11341 cttagtaaac aaatggaaag cagctggata tgctgaaatg tctttagaaa tcagaaaaaa
    11401 agactggaaa ttcgtagatg atcatctcct gatcgaaacg tcttatcgag gagttattcc
    11461 aggattacag ccaattttta caatgcacat acagttgaaa ataaatggtg caggcgtaat
    11521 caaccaaaaa gtatcagtgg agaaaaatcc gatttttcca gatttccctc gctttgggat
    11581 ggactggccg ttgaatgata ctttttcgaa ggtttcttat tgtggctatg ggccgtatga
    11641 aagttatcct gataagcact atgctgcgat gtttggtatt tatgagatgg agattgagga
    11701 atttgatcag ccatatatca aaccacaaga atatggtaac cgctggcaat gtgattgggt
    11761 atcaatcaaa aatcatcaag gatctaccat gacggtttat ggaaactcta atttttctgc
    11821 tctgccttat agtcaagctc aattaaccga aaagtcacac cattatgaat taataaaaga
    11881 aaatagaact tggttcaatt taagttataa acaaaatggt atcggctcac acagttgcgg
    11941 tccttattta gaagatcgtt tccgttttat ggaagatact tttgactgga atatagtgat
    12001 tactttcaaa ggagaaaata gatgaaagaa acaaagatca atcaagtcaa aaaaaaatta
    12061 tctcgttatc aagaagttgt ttatcgaaac cattgcttaa aatggtcaaa aaataagctg
    12121 aatgttcgtt ttaatggatt attttatcgt ttcgaacagt tagaagaagt ctggaaaccg
    12181 gtaacgaatt tccaatcatg tcctgtacag ctcataaatg atccaaaaga aacatacgat
    12241 ttatgtataa cacaccttta taaccagcat ttgaaaaatg acggatttta tttaaaggtt
    12301 actaatgaca atcaacttac gattgaatca gcaaaccttc gagggtttca atatgctatg
    12361 gaagccttat tagagagttt tttttcggta ggagatcaat tattattacc ggaattgatt
    12421 ctgaaacatg aaccatcagt gaaaattcga ggaatcattg aagggtttta tggtatcccc
    12481 tggacacatg aaatgcgcct ctctcttttt tcatatatga aagataacca attgaatact
    12541 tttatgtatg cccctaaaga tgatgaatta ttacgtaaaa aatggcgtga actctacgat
    12601 gcgcaagaat tagacaaatt taaggagctt ttatctgctg ctgaggctag caatatcgat
    12661 ttttggtact taattagtcc tggaaatgat attgatatta cgtgtgaaga agacattcaa
    12721 gttcttctga aaaagttaga acaactgatt gagcttggcg tatttcaatt tggtctatta
    12781 atggatgata ttgactatca attaaaaggt gctgctaaac gccgttttcg agagccagca
    12841 ttcgctcatg cctatttagt gaatcgagta gaggaatatc ttagcactcg gttgtccaat
    12901 catgaattag tcatttgtcc tacagaatat gataatcgtc atggttctcg ctatttagca
    12961 acgttaagtc aagaaatacc agaacaatta ccgttctttt ggactggccc aagcacgtta
    13021 gcggcgtcaa tttctacaga agagctgcag gaaatggctt ctgtctatca acggccgatg
    13081 ctgatttggg ataatattcc ggtaaatgat tatttagagg ataaagaact attgttcatg
    13141 agtccatatg agaatcgaac accgaatctt tctaaggaga gataccaggt tacaggagta
    13201 gtttcgaatc caatggctca attagaagct tcaaagttta cgatcaatag tatggctaat
    13261 tacttatgga actgtgagcg ttttgatcct ttggaaactt ggacgtcagt cgttgaaaaa
    13321 gtagttggaa gtaaactaca accagcttat cttaccttaa ccaatgcttt tccaaatcat
    13381 tacacaagca gtctgctttc agatgaagaa ttactgctag ccaaagaccc agaatgggtc
    13441 acaaaagaaa tggctgcttt agtccaagca gtaaaatgga tccagcaaga acaacataca
    13501 tctagattga gaacagaatt ggagccttgg ttacaagcaa tcacagaaag ttggactttc
    13561 tggcaagaat ggcaattaaa aaaagatcca aaagaagcag aagaatggtt ggctaagaaa
    13621 aaaacacacc gcataggacg tgatttagta actgcccatt tagagcaaat tatcaaaagt
    13681 tagatatctt aaaaaataaa aagcaagaaa aaggagaaca aaatggtcaa gagattaatt
    13741 agtgcaaact acagtgatgt caagaaaatg acgtcaaaag agttgaagca atccatcaaa
    13801 gcgagtgaag gacgggtgat tgtttgtgaa acggttgcta atactcttcc acaatctgga
    13861 gaaattacga atgcagaagt tgcgtcagca tttggtgcag atttgattct tttaaatggt
    13921 ttcgattgtt atcgtcctgc agtttttgga ctaccaaatg tgacgattga gaaggttttt
    13981 gatctacagc aaacgattga aaacccgatt cctaaattaa aagaactagt cggtcgtcct
    14041 gtgggaatta atttagaacc cattgcccct aaaacaaata tgatgagtga gaaagtagaa
    14101 atcagccaag gaagagcaag ctctgttgaa acgatacaaa aagcagaaga actaggagtc
    14161 gattttattt gctttaccgg taatccaggc actggagtaa ccaatttaga aattactaaa
    14221 tccgtaaaaa tggcaaaaaa gtatttttcg ggattgatta ttgctgggaa aatgcatagc
    14281 gccggctcag aagagccagt tgtgaataaa gaggcgatta tttcttatgc ggaagcaggc
    14341 gcagatattt tactagttcc ttcagtaggg tctatcccag gctttttaga gcaagaccta
    14401 attgaagcgg ttcggttggc aaaaaaatat gatttgttaa cgatgtctgc aatcggtact
    14461 agtcaggaaa gtgcccgacc agaaacattg cggcaattag ccattatgaa taaaagttgt
    14521 ggtgttgatt tacaacatat tggagatgct ggatacgggg gattggcacc agcagaaaat
    14581 attttcgaaa cctcggttgc aattagagga atgcgacata ccatcaactg cgttagccgc
    14641 tcgatcaatc gctaacaaaa ttaaataaaa aatggttaaa ttgattattt aagtgggaac
    14701 atacttgaga tatcagctaa cttctgtcac tttaaaaaag ggactgtgac agaagtgaaa
    14761 atagacaaaa aatatccgaa caagagacta ggatagcaga aaaactatcg ttttgcaacg
    14821 aggaccaact ggaccgtagg agcaattctc ttattgttcg gatatttcgc ttgttcaagg
    14881 acttatgtct gtagtgccct tttttggtag gaaaaatggt agggaaccat atcattttta
    14941 atgaaaatca agcatttcaa aagt
//
LOCUS       DACDXO010000063        14939 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E23605_2017
            SAMN12639989-rid9881463.denovo.064, whole genome shotgun sequence.
ACCESSION   DACDXO010000063 DACDXO010000000
VERSION     DACDXO010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639989
            Sequence Read Archive: SRR10023389
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741914.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:42:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,972
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 33 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 17 of 73
            Pseudo Genes (multiple problems)  :: 10 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14939
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639989-rid9881463.denovo.064"
                     /isolate="E23605_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..562
                     /locus_tag="IWM35_002183"
     CDS             <1..562
                     /locus_tag="IWM35_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAP9920023.1"
                     /translation="LKNEFGIEVKDYFIDLDLLQLINSVKRMYSGDDNGVDLYKFVTK
                     HIFVNLNIDEKNYPSIFKHYLFFINEVEDRIKNNSLSSSVSSFKKMFKSRDGITLTTA
                     HKVKGNEYDTVIAINLLNGKIPHWNEIFNVSDKGISSARKLLYVICSRAKNNLFLFSE
                     VGYFTSKGDLLYPTQLLDKIEFDYNI"
     gene            762..1076
                     /locus_tag="IWM35_002184"
     CDS             762..1076
                     /locus_tag="IWM35_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018705633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAP9920024.1"
                     /translation="MELKYVIPNMEKTFGQLEYAGEGNIEQRRINGRPTILSRSYNLY
                     STIQRADDIVVILPSEAGEKHFESDEKVKLINPHITAEGYKIGNRGFTNYIMHADDMV
                     KA"
     gene            1097..1474
                     /locus_tag="IWM35_002185"
     CDS             1097..1474
                     /locus_tag="IWM35_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAP9920025.1"
                     /translation="MRLAEGIVIEKDQTFGTLKFSALRREVRIQNEDGTVSEEIKERT
                     YDLKSKGQGRMIQVSIPASVPLKEFDYNAEVELINPVADTVATATFRGADVDWYVKAD
                     DLVLKGQTKPHQKYKKEASESEK"
     gene            1484..2257
                     /locus_tag="IWM35_002186"
     CDS             1484..2257
                     /locus_tag="IWM35_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9920026.1"
                     /translation="MTNTPLILKEIPKDEAISFIRQYHYSKILPRLCKYFLGIFSEEK
                     LLGVVELGWGTQPLQTIRKLFPDSSLQTTDYLEIGKMCFLPEMNQTNYFGSQALSALI
                     KWLKEHTDCHFLYTLADGIEGKCGYVYQASNFFYCGYFKTSVYRDKQSWEKIHPRSAR
                     LLLEENARFEQVEKKHWLSQAFCEYKGIEKINGRMFRYLYPLTKEAKKLLGHTLYRRH
                     YYPKEKNLRFEKRIAYQKYEAISQPTFDKQARIYNTQLF"
     gene            2279..3682
                     /locus_tag="IWM35_002187"
     CDS             2279..3682
                     /locus_tag="IWM35_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001130245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HAP9920027.1"
                     /translation="MPLLHDRGERIKANDKHLVYHFVLISLLVVFLVVLLPFHWRALQ
                     SVNWQAVQLDTFFYQLHTPYFWSSILTALFVCGLISWLYYHYRIDSIKQLEHRQKLAR
                     MILENHWYEAEETQSEGFFKDLPSKKTKQRIRHFPKIYYRMKDGLIHIHVEITLGKYQ
                     DQLLHLENKLETGLYCELVEKTLKDSYVEYVLLYDTIGNRIGIEDVVAQDGRVKLMES
                     VYWEFDSLPHMLIAGGTGSGKSYFILTLIESLLHTNAKLYILDPKNADLADLGAVMNN
                     VYYRKEDMLACINQFYEDMIDRSETMKQHPNYKTGENYAYLGLSANFLIFDEYVAMMD
                     MLGRESTTVIHKLKQIVMLGRQAGFFLILACQRPDAKYFSDGIRDNFNFRVALGRMSE
                     MGFGMMFGSDTQKQFFLKPIKGRGYVDTGKSVISEFYTPLVPKRYDFLGEIGKVIQKK
                     QSEPVPHEVKGTGSDLL"
     gene            3864..5048
                     /locus_tag="IWM35_002188"
     CDS             3864..5048
                     /locus_tag="IWM35_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000398284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAP9920028.1"
                     /translation="MENEKIWIKELKEKRLAYGVSQNKLAVASHITRPYLSDIETGKA
                     VPTQQVKEDLLNALERFNPDNPLEMLFDYVRIRFPTNDVAQIIGEVLRLNMDYMLHED
                     FGYYSYPEHYRFGDIVVLVSHDVSKGVLLELKGKGCRQFENFLLAQHRSWYDFFQDCM
                     EHKGIFKRLDLAINDKTGILNIPDLAKKCKQEECISVFRSFKNYRSGELVHRDEKPYM
                     GNTLYIGSLKSEVYFCIYEKDYEQYVKNDVPLEDAEVKNRFEIRLKNDRATQAMKDLL
                     AHQQAEKTAFEIINRYIRFADKDDTKRRSDWKTNERWEWFIGKNRGALRLTTQPEPYS
                     FERTLNWLHHQVAPTLKIASILDVLNGTTIISTMIQEAKLTEKHEKLIEQQHLAMEDL
                     IT"
     gene            5045..5335
                     /locus_tag="IWM35_002189"
     CDS             5045..5335
                     /locus_tag="IWM35_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000055367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9920029.1"
                     /translation="MSLLTKELKKLGFQCGIEFQAYIQNTGKYTSLIIEGKRQAGDTI
                     YTYDFYKVRFYNNYTNRVTVYGEHLTPFQLLRRVKSYIYYREKYLKERRTIT"
     gene            5332..5553
                     /locus_tag="IWM35_002190"
     CDS             5332..5553
                     /locus_tag="IWM35_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9920030.1"
                     /translation="MNFGQNLYNWFLDNAQSLVLLAIVVIGLFLGFKREFSKLIGFLI
                     IALIAVGLVFNAAGVKDVLLNLFNRIIGA"
     gene            5595..6374
                     /locus_tag="IWM35_002191"
     CDS             5595..6374
                     /locus_tag="IWM35_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000678782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="abortive infection family protein"
                     /protein_id="HAP9920031.1"
                     /translation="MKGFMNMFTNDQFKSLLIKTLERKSSSNYYEGGNEIVSKMKISE
                     VTLDETDDFTYYKGYKKGWNTLCTYLKIRVPFDDLDFFESHKDLITQTASSIYDKQGD
                     NVLVDTILVPLPENYEVINFSQLKISDVVSQAIEDAESFMSNGEYQRAFDRVHTAFHG
                     YLIEILKKYEITVPRDENLSKLYSRIQQLIEKEIQPTELADIVKTTIRSSNGMISSLN
                     EARNRHSLAHPNTNIIGKREAKLIIGISSTVTDYISGYLDK"
     gene            6441..6935
                     /locus_tag="IWM35_002192"
     CDS             6441..6935
                     /locus_tag="IWM35_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000870467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HAP9920032.1"
                     /translation="MKVYVANLGRYNEGELVGAWFTPPIDEEEMAERIGLNEDYEEYA
                     IHDFELPFDVDEYTPISEINRLCEAIQEIEGTPIYNELKEIQGMWFSSLEELLENKED
                     IHCYSDCDSMEDVARYYVEETGQLGEVPSNLQNYIDYQSLGRDMEIEGNYLVTSHGVF
                     EYCQ"
     gene            6991..7629
                     /locus_tag="IWM35_002193"
     CDS             6991..7629
                     /locus_tag="IWM35_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9920033.1"
                     /translation="MVNVFENLKLLKLDMKDKGWVIDSFYFRYKQQNYIVLVKLFEKE
                     EKVPEYALLKLEFLKENDFSDMLAVYANSVKLYTDTKTIREYFGIEYSSNLGDVLFQF
                     SQTLARFIPTEVSEKKNEDQKEAMCFSLSQSDSEDPRKKYCFSVRRNPLKGNGELGKR
                     SPFNDNKTRLYRPSLYERLGSDTNLSFRYSMNPDDEETDEGIIAKWTKNKIE"
     gene            7701..8096
                     /locus_tag="IWM35_002194"
     CDS             7701..8096
                     /locus_tag="IWM35_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP9920034.1"
                     /translation="MKKIRSYTSIWSVEKVIYAINDLQLPFPITFTQMTWFIVSLFVV
                     ILFGSLPPLSLIDGALLKYLGIPIVLTGFMCQKTFDGKKPYGFLKSIITYALRPKVTY
                     AGKQIRLKKETMNESITIVRSEQLDGISD"
     gene            8080..10533
                     /locus_tag="IWM35_002195"
     CDS             8080..10533
                     /locus_tag="IWM35_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAP9920035.1"
                     /translation="MAYPIKYMEDNLVFNHDGEVFAYYELIPYNYSFLSSDEKIQVHD
                     NFCQLIAQNRDGKIHALQLATESSIRATQEQSKKEITGRLKEIAYEKIDEQTEALISM
                     IGDNQVDYRFFIGFKLYLNEQEVSVKNVGKEIQKAFAEFFHGVNHKLMGDFVSMPMAE
                     IERFSKMEQLLESKLSRRFQIRKLEKDDFGYIIEHLYGQTGVPFDDYDYHLPKRYEDK
                     KVLVKKYDILKPTRCLIEENQRYLRIEQEDTTTYVAYFTINSIVGELDFPNSEIFYYQ
                     QQQFTFPVDTSMNVEIVANKKALSTVRNKKKELKDLDSHAWESDNETSTNVVDALESV
                     DELESNLDQTKESMYKLSYVVRVSAKNLDELKQRCDEVKDFYDDLSIKLVRPFGDMLG
                     LHNEFIPSSKRYINDYIQYVTSDFLAGLGFGATQMIGENSGIYVGYNLDTGRNIYLQP
                     SLASQGIKGSVTNALASAFIGSLGGGKSFSNNMLVYYSVLFGGQATIVDPKAERGEWK
                     ETLPDIAHEINIVNLTSENDNKGLLDPYVILKLPKDSESLAIDILTFLTGISSRDSEK
                     FPVLRKAIRSVTQSKQRGLLLVIDELRKEDTPISNSIADHIESFVDYDFAHLLFSDGS
                     VTQSISLEKQLNIIQVADLVLPDSNSSFEEYTTMELLSVAMLIVISTFALDFIHSDRS
                     IFKIVDLDEAWSFLQVAQGKALSMRLVRAGRAMNAGVYFVTQNADDLLDEKMKNNIGL
                     KFAFRSTDLVEIKKTLEFFGVDKEDENNQKRLRELENGQCLVSDIYGRVGVMQFHPIF
                     EELLHAFDTRPPVRNEVEE"
     gene            10530..12557
                     /locus_tag="IWM35_002196"
     CDS             10530..12557
                     /locus_tag="IWM35_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9920036.1"
                     /translation="MTQKQKKFLHYSWITLLCVGVILLLLGTLGNAVQATGLVDETID
                     TSNEYSKYGLNQYQLDYYVDNSWGWLPWNWSDGIGQSVMYGLYAITNFIWTISLYLSN
                     ATGYLVKEAYALDFISQTSQAIGENMQTIAGITPNGFSSSGFYVGFLLLFILIVGIYV
                     AYTGLVKRETTKVIRAVLNFVVVFILSASFIAYAPDYIAKINDFSKDVSTASLNIGTK
                     IVLPNTDSQGKDSVDMIRDSLFSVQVKQPWLLLQYGSTDVEALGEERVESLLSTSPDT
                     NNGEDRENVVIEEIEDHDNTYLTLPKTISRLGTVFFLFVFNIGISIFVFLLTGIMIFS
                     QVLFIIFAMFLPISFLLSMLPSFDGMGRHAITKLFNVIMTRAGITLIITVAFSISTML
                     YSLSASSPFFMIMFLQIVTFAGIYFKLGDLMSLFTLQSNDSQNMGRQVFRRPRQMMNR
                     QTRRFQRSMSKIFRGKAGSTKQKTMTTKQNQTNKANHTRQNERTTPKKETALSNVKKQ
                     QGGIGQRLGEKTAHVLDTKNRLVNKRKQAKQQIQATPTHVKYALHQGKENVTHNVTDF
                     KQSMADTKAQRKQQRMNQQEQKRKNIAQKRLELDKAKEKRRKENPSSPVQNNQQHVRK
                     RQQTVTPVLSQNTKRENHQRPLKKEQKQKTPPKQFNQRKLNKRTDKRGQQT"
     gene            12554..13576
                     /locus_tag="IWM35_002197"
     CDS             12554..13576
                     /locus_tag="IWM35_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase P60"
                     /protein_id="HAP9920037.1"
                     /translation="MKLKITVICSVVFSLFFFLLLFLVIFFADDTDSGENNKDSSIPQ
                     GGVTVSPEVLAHRPLIEKYGKEYGIEDYVSYILAIMQVESGGTAEDVMQSSESLGLPP
                     NSLSTEESIKQGVKYFSELLTSAEQQGVDIDSVIQSYNYGGGFLNYVRSHGKKYTYEL
                     AEQFSKEKSGGQKADYPNPIAIPVNGGWRYNYGNQFYVQLVSQYLTDTSPTEFDDETV
                     QVIMDEALKYEGFPYVFGGASPTTSFDCSGLIQWVYDKAGISLPRVAQDQYDATQEIS
                     MEEAQAGDLIFFHSTYNAGTYVTHVAIYLEGNRFYHAGDPIGYGDLSSRYWQDHLIGA
                     RRVIHN"
     gene            13593..14522
                     /locus_tag="IWM35_002198"
     CDS             13593..14522
                     /locus_tag="IWM35_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP9920038.1"
                     /translation="MIIKIERKEKKEKPKKSKKPSKQRKVPMIKVGTHKKLTFVLWVL
                     LIGSVGFGIYKNFTAIDTHTVRETEIIKQQIVDTNQVESFVKSFAKDYFSWQQSQEAI
                     DKRNEKLTHYLTEELQVLNEEMIRKDIPTSSSVNDIQVWQVSQVNENTFEVLFSVEQV
                     ITEDKDKETISSSFHVVVHIDESDNMVIIKNPTMSKKPQKSDYQPKQLESDHTVDTET
                     MDEITSFLETFFQLYPTATEKELTYYVSNHVLPMINKEYVFEELVNPIFTRKDNQVIV
                     NVAVKYLDQETKATQISQFELILEKQDNWKIVK"
     gene            14767..14883
                     /locus_tag="IWM35_002199"
     CDS             14767..14883
                     /locus_tag="IWM35_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HAP9920039.1"
                     /translation="MILKYPENICMLCIPMVMHKNPSDKSIYHWDFYALLGF"
BASE COUNT         5072 a         2485 c         2954 g         4428 t
ORIGIN      
        1 attaaaaaat gaatttggaa tagaagttaa agattatttt attgacttag atttattaca
       61 gctaataaac agtgtgaaaa gaatgtatag tggagacgac aacggtgttg atttatacaa
      121 atttgttacc aaacatatat ttgttaatct taatattgac gagaaaaatt acccgtctat
      181 ttttaagcac tatctatttt ttattaacga agtagaagat cggataaaaa ataattcatt
      241 atctagtagt gtatctagtt ttaagaagat gtttaaatct cgagatggta tcacacttac
      301 tacagcacat aaagttaaag gaaatgagta tgatactgtt atcgctatta atcttctaaa
      361 cgggaaaatt cctcattgga atgaaatttt taatgtttct gataaaggaa ttagttcagc
      421 gagaaaacta ctatacgtaa tttgttcaag agcaaaaaat aatttatttc ttttctcaga
      481 ggtgggttat tttacgagta aaggagactt gctttatccc actcaattat tagataaaat
      541 agaatttgat tataatattt aaaataccta ggtagtcgtt gttttaaata tagtaggaat
      601 cggacgctca gtacggagcg tctatttttt tacccaattt tgaaaggaag tgaacttatg
      661 aaaataaaaa atcaagaatc aaaaggtcgc tctcccccct ttaaaaccat cacacaaata
      721 agattcattc aaacataaaa acagaaagga taggtaaaaa tatggaactt aaatatgtca
      781 ttcccaatat ggaaaaaaca tttggacaat tagaatatgc tggtgaaggg aacattgaac
      841 aacgtcgaat aaatggcaga ccaaccattc tctctcgtag ttacaattta tactcaacga
      901 ttcaacgagc agatgatatt gttgtcattc ttccctccga agctggcgag aaacattttg
      961 aatccgacga aaaagtcaaa ctcatcaacc cacatattac cgcagaaggt tataaaattg
     1021 gcaatcgagg atttaccaat tacattatgc acgcagacga tatggtgaaa gcataagaaa
     1081 ggaaaggtga aaaaatatga gattagcaga aggtattgtg attgaaaaag accagacatt
     1141 tggcacattg aaattttccg ccttgcgtcg tgaggttcgt atccagaatg aagatggtac
     1201 ggtttccgaa gaaattaaag aacgtaccta tgacttaaaa tcaaaaggac aaggacgcat
     1261 gatacaagtc agtatccccg cttctgttcc tttgaaagaa tttgactaca acgcagaagt
     1321 ggaattaatc aatcctgtgg cagataccgt agcgacggct acttttcgag gggcagacgt
     1381 tgattggtac gtgaaagcag acgatcttgt attaaaagga cagacaaaac ctcatcagaa
     1441 atataaaaaa gaagcgtccg aaagtgaaaa ataggtgttg cttatgacaa acacaccatt
     1501 gattttgaaa gaaattccga aggatgaagc catttctttt attcggcaat accattattc
     1561 aaagatactc cctagattgt gtaaatactt tctagggatt ttttcagaag aaaaattatt
     1621 aggcgttgtg gaattaggct ggggtacgca acccctacaa accatacgca aactctttcc
     1681 cgacagttcc cttcaaacca cagattattt agaaattgga aagatgtgtt ttcttccaga
     1741 aatgaatcaa accaattatt ttggcagtca agctctttca gctctaatta agtggttgaa
     1801 agaacatacg gactgtcatt ttttatatac actggcagat ggaattgagg gaaaatgtgg
     1861 ctatgtctat caagcgtcca acttctttta ctgcgggtat ttcaagacaa gtgtctatcg
     1921 tgataagcag tcatgggaaa agatacaccc tcgaagtgct cgccttttat tagaggaaaa
     1981 tgctcgcttt gaacaggtag agaaaaagca ttggctttcc caggcatttt gcgaatacaa
     2041 agggattgag aaaatcaatg gacgcatgtt tcgctatctc tatccgttga caaaagaagc
     2101 gaaaaaactg ttaggacaca ctctctatcg acgtcattat tatccgaaag aaaaaaattt
     2161 acgctttgaa aagcgaatcg cttatcagaa atatgaagcc atttctcaac caacttttga
     2221 taaacaggct cgtatctaca atacacaatt attttgatag gtggaaagga ggatttttat
     2281 gccattgctt catgacagag gagagcgaat caaagccaac gataagcact tggtctatca
     2341 ttttgtacta atttcattgc tggtagtttt tctagtcgtc ctactccctt ttcattggag
     2401 agcgttacaa tcggtgaact ggcaagcagt tcagttagat acatttttct atcaacttca
     2461 tacgccatac ttttggagca gtattctcac tgctcttttt gtatgcggac ttatcagctg
     2521 gctttattat cactatcgta tagattctat aaaacagtta gagcaccgtc aaaagttagc
     2581 tcgtatgatt ttagaaaatc attggtatga agccgaagaa actcaatcag agggattctt
     2641 taaagattta cccagcaaga aaaccaaaca acgaatccgc cattttccta aaatatatta
     2701 tcgaatgaaa gatggactaa tccatattca tgtagaaatc accttaggga aatatcaaga
     2761 tcagcttttg cacctagaaa acaaactaga aacaggattg tattgtgaat tagtggaaaa
     2821 aacgttgaaa gattcttatg tagagtatgt cctactttat gatactattg gcaatcgaat
     2881 tggcattgaa gacgtggtag ctcaagatgg acgtgtcaaa ttgatggaat cggtctattg
     2941 ggagttcgat tctctccctc acatgttaat tgctgggggc actggtagtg gaaaatccta
     3001 ttttattctc acattgattg aatctctcct gcatacaaat gcaaagcttt atattcttga
     3061 ccctaaaaat gctgatttgg cagacttagg ggcggtcatg aataatgtct attaccgtaa
     3121 agaagatatg ttggcatgta tcaatcaatt ttacgaagat atgattgatc gcagtgaaac
     3181 catgaaacaa caccctaact ataaaacagg agaaaattat gcttatcttg gtttgtctgc
     3241 caattttctt atttttgatg aatatgtggc aatgatggat atgctaggtc gtgaaagtac
     3301 caccgttatt cataaactca aacaaattgt aatgttagga cgacaagcag gcttcttttt
     3361 aattcttgcc tgtcaacgtc cagacgcaaa atattttagt gatgggattc gggacaactt
     3421 taacttccgt gtggctttgg gtcgtatgtc tgaaatgggc tttggtatga tgttcggaag
     3481 cgatacacaa aaacaatttt tcttaaaacc catcaaggga agaggttacg tggatacagg
     3541 aaaaagtgtg attagtgagt tttatacacc ccttgtacca aaaaggtacg atttcttagg
     3601 agaaatagga aaagtcattc aaaaaaagca atctgagcct gtaccgcacg aagtgaaagg
     3661 tacaggttca gacttgcttt gattggtgtg gcgttagcca cgccaatcct ttagccccct
     3721 ctatctaaca ggggggcaca aatacaactg aaaacaggca attctcatca accaatgttg
     3781 atggtacaaa ggattgcgga aaatatgaaa ggtgtaaact caagggtcat agtttacatc
     3841 ttttttagat tggaggtttg cgaatggaaa acgaaaaaat ttggattaag gaattgaaag
     3901 aaaagcgact ggcttatggc gtttctcaaa ataaactggc ggttgcttct catattacca
     3961 gaccttacct atcggatatt gaaacaggca aggcagtccc tactcaacag gtgaaagaag
     4021 atttactgaa cgctttggaa cgcttcaatc ccgataaccc attggagatg ttattcgact
     4081 atgtacggat tcgattccct accaatgatg tggctcaaat tatcggtgag gttttacgtt
     4141 tgaatatgga ttatatgcta cacgaagact ttggctatta ttcttatccc gaacactatc
     4201 gtttcggtga tattgttgtc ctagtctctc atgatgtaag taaaggtgtt ttattagaac
     4261 tgaaaggaaa aggctgtcgg caatttgaaa actttctatt ggctcaacat cggagctggt
     4321 atgatttctt tcaagattgc atggaacata aaggtatttt caaacgcttg gatttagcta
     4381 tcaacgataa aacagggatt ttgaatattc cagatttggc aaagaaatgt aaacaggaag
     4441 aatgcatttc cgtctttcga agctttaaaa attatcgcag tggtgaattg gttcaccgtg
     4501 atgaaaaacc atacatggga aacacacttt atattggctc attaaaaagt gaagtctatt
     4561 tctgtatata tgagaaagat tatgagcaat acgtgaaaaa tgatgttccc ttagaagacg
     4621 cagaagtaaa gaatcggttt gaaattcggt taaaaaatga ccgagccacc caagccatga
     4681 aagatttatt ggcacatcaa caggcggaaa aaacagcctt tgaaattatc aatcgctata
     4741 tccgctttgc agataaagac gacactaaac gtcggagtga ttggaaaact aatgaacgtt
     4801 gggaatggtt tatcggtaaa aatcgtggag cattacgcct aaccacacag ccagagcctt
     4861 attcttttga acgaacattg aactggttac atcatcaagt cgcccctaca ttaaaaatcg
     4921 caagcatttt agatgttctc aacggtacaa ccattatctc caccatgatt caggaagcca
     4981 aactgacaga aaaacatgag aaattaattg aacaacaaca tctagccatg gaggatttga
     5041 ttacatgagc cttttaacaa aagaattaaa gaagttaggg tttcagtgtg gcattgagtt
     5101 tcaagcgtat atacaaaata cagggaaata tacgtctctc attattgagg gaaaacggca
     5161 agctggtgat accatctata cctatgattt ttacaaggtg agattttata ataattatac
     5221 caatcgagta accgtctatg gagagcattt aacccctttt caattattaa ggcgtgtaaa
     5281 aagctatatc tattatcgag aaaaatatct taaggaaagg aggacaatca catgaatttt
     5341 gggcagaatt tgtacaactg gttcttggat aatgcacagt cattggtatt gttagcgatt
     5401 gttgtcattg gactgttttt aggttttaaa cgagaatttt caaaactcat tggcttcttg
     5461 attattgctc taatcgccgt cggacttgtg ttcaatgcag ctggcgtgaa agatgtgtta
     5521 ttaaacttat ttaaccgaat cattggtgct taacagatat ttttcatgat aaaataggaa
     5581 gtatatattt tgttatgaaa ggatttatga atatgtttac aaatgatcaa tttaaatctc
     5641 ttttaattaa aacattagaa agaaagagct cttcaaatta ttatgaagga ggtaacgaga
     5701 ttgtttctaa aatgaaaata tcagaggtaa ctttagatga gactgacgat tttacttatt
     5761 ataaaggata taaaaagggc tggaatacgc tttgcacata tcttaaaata agagttcctt
     5821 ttgatgattt agactttttt gaaagtcata aagatttgat tacccagaca gcaagctcca
     5881 tatatgataa acaaggtgat aatgtattag tggatactat tttagtacca cttccagaga
     5941 attacgaagt aattaacttt agtcagttga aaattagtga tgtggtttca caagctattg
     6001 aagacgctga gtcttttatg agtaatggtg aatatcaacg tgcctttgac agagttcaca
     6061 ctgcttttca tggatattta attgaaatac taaaaaaata tgaaattaca gttcctagag
     6121 acgaaaatct atctaaatta tatagtagga tacaacaatt aattgaaaaa gaaattcaac
     6181 ctactgaact tgctgatata gtgaagacaa cgataaggtc atcaaatgga atgattagct
     6241 ctcttaatga agcaagaaat aggcattcac ttgcacaccc taatacaaat attattggaa
     6301 agagagaagc aaaactaata ataggtattt caagtacagt tacagattat attagcggat
     6361 accttgataa gtagcgtcat taactgattg ctactttttt tatgactatt tttataaaaa
     6421 ggatgtgata agaattggaa atgaaagtct atgtggcaaa tttaggacgg tataatgaag
     6481 gtgaattagt cggagcatgg tttactccac caattgatga ggaagaaatg gcagaaagaa
     6541 tcggtttaaa tgaggactac gaagaatatg caatacatga ttttgagctt ccttttgatg
     6601 tggacgaata cacgccgatt agtgaaatca accgtctatg tgaagccatt caagaaatcg
     6661 aaggtacacc tatttataat gaactcaaag aaattcaagg catgtggttt agtagcttgg
     6721 aagaattact ggaaaacaaa gaggatattc attgttatag tgattgtgat tcgatggaag
     6781 atgtcgcccg ttattacgtg gaagaaacag gacagcttgg tgaagtccct tccaatttac
     6841 aaaattatat tgactaccaa tctttgggac gtgatatgga aatagagggt aattacctag
     6901 ttacctctca tggggtattt gaatattgtc agtaagttta acaatccgtg ttataattaa
     6961 attaataaat agcacggaga ggtataaatt atggtaaatg tatttgaaaa tcttaaacta
     7021 ttaaaactag acatgaaaga taaaggctgg gtgatagatt ccttttactt tcgttacaaa
     7081 cagcaaaatt atattgtact tgttaagcta tttgaaaaag aggaaaaagt tccagaatat
     7141 gctctgttaa aactagaatt tttaaaggaa aacgattttt cagatatgct agctgtctat
     7201 gctaattcgg taaaattata tacagacacg aaaacaataa gagaatattt tggtatcgaa
     7261 tacagtagta acttgggaga tgttttattt caatttagcc aaactcttgc tcgatttatt
     7321 ccgacagaag ttagtgagaa gaaaaatgaa gaccaaaaag aagccatgtg tttctcatta
     7381 agtcaaagtg attcagaaga tcccagaaaa aaatattgtt tttcagttag aagaaatcct
     7441 ttaaagggaa atggtgagct gggaaaacga agtcctttta atgacaataa aacgagattg
     7501 tatagaccca gcttatatga acgattaggg tcagatacca atttaagttt cagatattca
     7561 atgaatccag atgatgaaga aactgatgaa ggtattattg caaaatggac aaaaaataaa
     7621 attgaatagt taaagcattg aaagagcaat cacctatttt gattgttctt tttctatttt
     7681 tacggaaagg acgtgaagac atgaaaaaaa taagaagtta caccagtatt tggtcagtag
     7741 aaaaagtgat ttatgcgatt aatgatttac aactcccttt cccaattacc tttacgcaaa
     7801 tgacatggtt tatcgtttct ttgtttgtcg tcatactctt tggcagttta ccaccacttt
     7861 ctctgattga cggagcacta ctcaaatact tagggattcc cattgttctc actgggttca
     7921 tgtgccaaaa gacctttgac ggaaagaaac cctatggatt tctaaagtct atcattacct
     7981 atgctctacg ccctaaagta acctacgctg gcaaacaaat acgattaaaa aaagaaacaa
     8041 tgaatgaatc gattacgatt gtaaggagtg aacaactaga tggcatatcc gattaaatat
     8101 atggaagata atctcgtatt taatcatgat ggcgaagtat tcgcttatta tgaattaatt
     8161 ccctataatt acagtttttt aagttccgat gaaaaaattc aagttcatga taatttctgt
     8221 caattgattg cccaaaatcg tgatggcaag attcatgctc tgcaactagc aaccgaatca
     8281 agtattcgag ccacacagga acaatcgaaa aaagaaatca ctggacgatt aaaagagata
     8341 gcttacgaaa aaatagatga acagaccgaa gcgttaattt ctatgattgg tgacaatcaa
     8401 gtagactatc gcttttttat tggttttaaa ctctacctca atgaacagga agtatccgta
     8461 aaaaatgttg ggaaagaaat tcaaaaagca tttgcggaat tctttcatgg tgtgaatcac
     8521 aaattaatgg gcgattttgt ttctatgcct atggcggaaa tcgaacgctt ttctaagatg
     8581 gaacagttgt tagaaagtaa attatccaga cgctttcaga ttcgtaaatt ggagaaagat
     8641 gattttggtt atattattga acatctttac gggcaaacag gtgtcccttt tgatgactac
     8701 gactaccatt taccgaaacg atacgaagat aaaaaagtgt tggtaaagaa atacgatatt
     8761 ctcaaaccca ctcgctgttt aatcgaagaa aatcaacgct atttacggat tgaacaggaa
     8821 gataccacta cttatgtggc gtattttaca attaatagta ttgttggtga attggatttc
     8881 cctaatagtg aaatttttta ttaccagcaa cagcagttta ctttccccgt tgataccagt
     8941 atgaatgttg aaattgtcgc caataaaaaa gccttatcta ccgttcgcaa taaaaagaaa
     9001 gaactgaaag atttagatag ccacgcatgg gaaagcgaca acgaaaccag tactaatgtg
     9061 gtggacgctt tagaatctgt cgatgaatta gaaagtaatt tagaccaaac caaagaatct
     9121 atgtacaaac taagctatgt ggtacgggta tcagcaaaga atttggacga gctgaaacaa
     9181 cgttgtgatg aagtaaaaga tttttatgat gatctgtcta tcaaattagt acgacctttt
     9241 ggcgatatgc taggcttgca taatgaattt attccctcaa gcaagcgata tatcaatgac
     9301 tatattcaat atgtcacgtc ggattttttg gctggtcttg gtttcggtgc tacacaaatg
     9361 attggtgaaa attctggtat ttacgtcggt tacaatctcg atacaggcag aaatatttat
     9421 ctccaaccta gccttgcaag tcaaggaatt aaaggctctg taacaaatgc attagcgtcc
     9481 gcttttattg gttcattagg tggcggaaaa tcattctcta acaatatgct ggtctattat
     9541 tcagtactct ttggcggaca ggcaaccatt gttgacccaa aagctgaacg tggtgaatgg
     9601 aaagaaacct tacctgatat tgcccatgaa atcaatattg ttaatctcac gagtgagaac
     9661 gacaataaag ggttacttga cccttatgtg attttaaaac tcccgaaaga ttcagaaagc
     9721 cttgccattg atattctcac gttcttaaca ggtatttcca gccgagatag cgagaagttt
     9781 cctgtcctgc gtaaagctat tcggagtgta actcaaagca aacaacgagg gttgttattg
     9841 gtgattgatg aattgcgaaa agaagatacg cccatcagca atagtattgc tgaccatatc
     9901 gaaagctttg tcgattatga ttttgcccat ctgttattca gtgatggcag tgtcacccaa
     9961 tccattagct tggaaaaaca actcaatatt attcaagtgg cagatttggt cttacccgat
    10021 tccaatagct cttttgaaga atacactacc atggaattat taagtgtggc aatgctgatt
    10081 gtcattagta cctttgcttt agactttatc cactctgacc gttccatttt taaaattgtt
    10141 gaccttgatg aggcttggag ttttttacaa gtggcacaag gaaaagctct atctatgcga
    10201 ttggttcgag caggacgtgc catgaatgca ggggtctatt tcgttactca aaacgcggac
    10261 gatttactag atgaaaagat gaagaataat attggcttga aatttgcctt tcgttccact
    10321 gacctagtgg aaatcaaaaa gaccttagaa ttttttggtg tggacaagga agatgaaaac
    10381 aatcagaaac gcttacgtga attagaaaat ggacaatgtt tagtttcaga catctatggt
    10441 cgtgtagggg ttatgcagtt tcacccgatt tttgaagagt tgcttcatgc ctttgatacc
    10501 agaccgcctg tgcgaaatga ggtggaagaa tgacacaaaa acaaaagaaa tttcttcact
    10561 atagttggat aacgctcctt tgtgtgggcg ttattttact tttattaggc acgctgggca
    10621 atgcagtaca agcgacaggc ttagttgatg aaaccattga tacaagcaat gaatactcca
    10681 agtatggttt gaatcaatat cagcttgatt attatgtgga caatagctgg ggctggcttc
    10741 catggaattg gagtgatggc ataggacaat cggtgatgta tggattgtat gccattacta
    10801 attttatttg gacaatttct ctttatctat cgaacgccac aggttatttg gtgaaagaag
    10861 cctatgcctt agatttcatt agtcaaacat cccaagcaat tggcgaaaac atgcagacga
    10921 ttgcaggcat tacaccgaat ggcttttcca gttcagggtt ttatgtcggt ttcctccttc
    10981 tttttattct cattgttgga atctatgttg cctatacagg attggttaag cgagaaacga
    11041 cgaaagtgat acgagctgtt ttaaactttg tcgtggtgtt tattctatcg gcttccttta
    11101 ttgcgtatgc acccgattat attgcgaaaa tcaatgattt ttccaaagat gtcagtaccg
    11161 ccagtctgaa tattgggaca aaaattgttc ttcctaacac ggatagtcaa gggaaagaca
    11221 gtgtggacat gatacgtgac agcttattct ctgttcaagt aaagcaacca tggctattgc
    11281 ttcaatatgg ctccactgat gtagaagcgt taggggaaga acgtgtggaa agtttattgt
    11341 ctaccagtcc agatacaaac aatggtgaag atagagaaaa tgtagtgatt gaagaaattg
    11401 aagatcatga caatacctat ttaaccctac cgaagacgat ttccagatta ggaacagtct
    11461 tctttttgtt tgtattcaat attggtatct cgatttttgt tttccttcta acaggaatca
    11521 tgattttttc gcaagtgcta tttattattt tcgctatgtt tttgccaatc agtttcctat
    11581 taagtatgtt gccgagtttt gatggcatgg gcaggcatgc cattaccaag ttatttaatg
    11641 tgattatgac aagggctggt attaccttga ttattaccgt tgcctttagt atttccacta
    11701 tgctttattc gctgtcagca agttctccct ttttcatgat tatgttttta caaattgtga
    11761 cctttgcagg gatttatttt aaactgggcg atttaatgag cctattcact ttacaaagca
    11821 acgactctca aaacatgggc agacaagtgt ttcgcagacc tcgtcaaatg atgaatcgac
    11881 aaaccagaag atttcaacgc agtatgagta agatttttcg tggaaaagca gggtcaacga
    11941 agcaaaagac aatgacaaca aagcagaatc aaaccaataa agctaaccat actcgtcaaa
    12001 atgaacggac tacaccaaag aaagaaacag cactttccaa tgtgaaaaag caacaaggcg
    12061 gtatcgggca acgattaggt gaaaagacag cccatgtatt ggacacgaaa aatcgtctag
    12121 tcaataaaag aaagcaggca aaacaacaga tacaggcgac accaacccat gtcaaatatg
    12181 cccttcatca aggaaaagag aatgtcactc ataatgtaac agactttaaa caatctatgg
    12241 cagataccaa agctcaacgc aaacaacagc gtatgaacca gcaggagcaa aaacgaaaga
    12301 acattgctca aaagcgtttg gagctggata aagcaaaaga aaaaagacga aaagaaaacc
    12361 catcatctcc tgttcagaat aaccagcagc atgttcgcaa gcgtcagcaa accgtcacgc
    12421 ctgttttgtc acaaaacact aaacgtgaaa atcatcagcg accattgaaa aaagagcaaa
    12481 aacaaaagac accaccgaag caattcaatc agaggaagct aaataaaaga acggataagc
    12541 gaggacaaca aacatgaagc taaaaataac agtgatttgt agtgtggtct tctctctctt
    12601 cttttttctc ttattattcc tagtcatttt ctttgcagat gatacggaca gtggcgaaaa
    12661 caacaaggat tcttctattc cacaaggcgg tgtgaccgtt tcaccagaag tgttggcaca
    12721 ccgtccgttg attgaaaaat atggtaagga atatgggatt gaagactatg tttcttatat
    12781 cttagccatt atgcaagtgg aatcgggcgg tacagcagaa gatgttatgc aaagttccga
    12841 atctttgggt ttaccgccca acagtttgag taccgaagag tccatcaaac aaggggttaa
    12901 atatttcagt gaattattaa ccagtgcaga acaacaagga gtggatatag attctgttat
    12961 ccaatcttat aattatggcg gtggcttttt aaattatgtg aggagtcatg ggaaaaaata
    13021 tacctatgaa ttagccgaac agttttctaa agagaagtcg ggtggtcaaa aagcagacta
    13081 tcccaatcct atagccatac ctgtgaatgg tggttggcga tataactatg gaaatcagtt
    13141 ttatgttcag ttggtttcac aatacctaac cgacacttcc ccaacagaat ttgatgatga
    13201 aaccgtgcaa gtcattatgg acgaagcctt gaaatacgaa gggtttccct atgtctttgg
    13261 aggggcgtcg cctaccacct cttttgattg tagtggctta atacaatggg tgtatgacaa
    13321 agctggcatt tctctccctc gtgtcgccca agatcagtat gacgctacgc aagaaatctc
    13381 tatggaagaa gcccaagcag gcgaccttat cttctttcat tcaacctata atgcaggcac
    13441 atatgtgact catgtggcga tatatttaga gggaaatcgc ttttatcatg caggcgaccc
    13501 aatcggttat ggcgacttaa gcagtcgtta ttggcaagac catctgattg gggctagacg
    13561 tgttattcat aactaagaaa ggaatgaatc aaatgattat aaaaatcgag cgaaaagaaa
    13621 agaaggagaa accaaagaaa tctaaaaaac cgagcaagca aagaaaagtt ccgatgatta
    13681 aagttggcac gcataagaaa ttaacctttg ttttatgggt tttattgatt ggtagtgtcg
    13741 gatttggaat ctataaaaat tttactgcca ttgacactca tactgtccgt gaaacagaaa
    13801 tcattaaaca gcagattgtc gataccaacc aagtagaaag ttttgtgaaa tcctttgcga
    13861 aagactactt ttcgtggcaa caatcccaag aagccattga taaacgaaat gaaaaattga
    13921 cccattattt gacggaagaa ttgcaggtat taaatgaaga aatgattcgt aaggatattc
    13981 ctactagttc tagtgtgaac gatatacagg tgtggcaggt ttctcaagtg aatgagaaca
    14041 cctttgaggt actgttttct gttgaacaag ttattaccga agacaaagac aaggaaacta
    14101 tatcgtctag ctttcatgtg gttgtccata tagatgaatc agataatatg gttattatca
    14161 aaaatccgac aatgagtaag aaacctcaaa aatcggacta ccaacccaaa cagctagaaa
    14221 gtgaccatac agtagataca gaaacgatgg acgaaatcac tagcttttta gaaacgttct
    14281 tccagctcta tcctaccgcc acagaaaaag aattgactta ctatgtaagt aatcatgtgt
    14341 tacctatgat taacaaggaa tatgttttcg aggaattggt gaatcctatc tttaccagaa
    14401 aagataacca agtaatcgtg aatgtcgctg tgaaatattt agatcaagaa acaaaggcaa
    14461 cccaaatctc gcaatttgag ttgatattag aaaaacaaga taattggaag attgtaaaat
    14521 aacaaatatt ggtacatgat tacagatact ttgtaatcat gtactctttt tgataaaaaa
    14581 ttggagattc ctttacaaat atgctcttac gtgctattat ttaagtgact atttaaaagg
    14641 agttaataaa tatgcggcaa ggtattctta aataaactgt caatttgata gcgggaacaa
    14701 ataattagat gtcctttttt aggagggctt agttttttgt acccagttta agaatacctt
    14761 tatcatgtga ttctaaagta tccagagaat atctgtatgc tttgtatacc tatggttatg
    14821 cataaaaatc ccagtgataa aagtatttat cactgggatt tttatgccct tttgggtttt
    14881 tgaatggagg aaaatcacat gaaaattatt aatattggag ttttagctca tgttgatgc
//
LOCUS       DACDXO010000064        14525 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E23605_2017
            SAMN12639989-rid9881463.denovo.065, whole genome shotgun sequence.
ACCESSION   DACDXO010000064 DACDXO010000000
VERSION     DACDXO010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639989
            Sequence Read Archive: SRR10023389
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741914.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:42:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,972
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 33 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 17 of 73
            Pseudo Genes (multiple problems)  :: 10 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14525
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639989-rid9881463.denovo.065"
                     /isolate="E23605_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(330..692)
                     /locus_tag="IWM35_002200"
     CDS             complement(330..692)
                     /locus_tag="IWM35_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9920040.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
     gene            complement(766..1146)
                     /locus_tag="IWM35_002201"
     CDS             complement(766..1146)
                     /locus_tag="IWM35_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HAP9920041.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            1228..2403
                     /locus_tag="IWM35_002202"
     CDS             1228..2403
                     /locus_tag="IWM35_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAP9920042.1"
                     /translation="MIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADMDFLSPG
                     GVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVLTSLAAA
                     VQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDMEKKIEEN
                     NVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHTFTSMLT
                     IDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEINTFGLI
                     GTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLDFTAYIS
                     DDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYALETID"
     gene            complement(2480..3064)
                     /locus_tag="IWM35_002203"
     CDS             complement(2480..3064)
                     /locus_tag="IWM35_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP9920043.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
     gene            3265..4263
                     /locus_tag="IWM35_002204"
     CDS             3265..4263
                     /locus_tag="IWM35_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP9920044.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            4375..4884
                     /locus_tag="IWM35_002205"
     CDS             4375..4884
                     /locus_tag="IWM35_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HAP9920045.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
     gene            complement(4914..5573)
                     /locus_tag="IWM35_002206"
     CDS             complement(4914..5573)
                     /locus_tag="IWM35_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HAP9920046.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQTKKKEL
                     V"
     gene            complement(5575..6339)
                     /locus_tag="IWM35_002207"
     CDS             complement(5575..6339)
                     /locus_tag="IWM35_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HAP9920047.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            complement(6347..7024)
                     /locus_tag="IWM35_002208"
     CDS             complement(6347..7024)
                     /locus_tag="IWM35_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HAP9920048.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            complement(7061..8446)
                     /locus_tag="IWM35_002209"
     CDS             complement(7061..8446)
                     /locus_tag="IWM35_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HAP9920049.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSYLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            complement(8415..8669)
                     /locus_tag="IWM35_002210"
     CDS             complement(8415..8669)
                     /locus_tag="IWM35_002210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9920050.1"
                     /translation="MSDYNETTTDFRVCFFYFYSTVKMNVPDFLARLIYRILYLYNLL
                     QGRKNSEIELVSMEEKAAYGMIKIKRGERVHGKNSDFTYE"
     gene            complement(8656..8740)
                     /gene="ffs"
                     /locus_tag="IWM35_002211"
     ncRNA           complement(8656..8740)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="IWM35_002211"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(8763..9278)
                     /gene="tadA"
                     /locus_tag="IWM35_002212"
     CDS             complement(8763..9278)
                     /gene="tadA"
                     /locus_tag="IWM35_002212"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HAP9920051.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            9385..10242
                     /locus_tag="IWM35_002213"
     CDS             9385..10242
                     /locus_tag="IWM35_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fis family transcriptional regulator"
                     /protein_id="HAP9920052.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYRYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDATEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            complement(10323..10658)
                     /locus_tag="IWM35_002214"
     CDS             complement(10323..10658)
                     /locus_tag="IWM35_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9920053.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(10725..12095)
                     /locus_tag="IWM35_002215"
     CDS             complement(10725..12095)
                     /locus_tag="IWM35_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NOL1/NOP2/sun family putative RNA methylase"
                     /protein_id="HAP9920054.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            complement(12173..12709)
                     /locus_tag="IWM35_002216"
     CDS             complement(12173..12709)
                     /locus_tag="IWM35_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP9920055.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
     gene            13018..14499
                     /locus_tag="IWM35_002217"
     CDS             13018..14499
                     /locus_tag="IWM35_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAP9920056.1"
                     /translation="MLIEARKKELLVLQMVIQKNNCSLQELSNDLHIPKRTIKELIRK
                     INVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLI
                     NYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLKKFHLRILSVKNCCTLEGNELSIR
                     LYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQ
                     QAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQ
                     ISFRQKQEIGFLFSTSKNLICRQVGQLMTYLTQQFPLNHLENKIPIYFYFLVLAVVFF
                     ELLGESSSSFSTLCFPEATSNHSSSFIQEKKLREKIDTIFSKNGERNQISQYLSQLIQ
                     HLLQTETKEQLLIHLQITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQHE
                     KISDETETFYLDTTHNTVLFGKLIKAVHELYMKKGYENKTKRM"
BASE COUNT         4475 a         2891 c         2358 g         4801 t
ORIGIN      
        1 actcagttat cttatcatgt cgttagtaac atgtcaataa ctatttttta aattttttag
       61 aaatgtgata taacatccac ttcagaatca acgctgttaa atttatcatg tatttcttcc
      121 ttggtcaact ataaattaag cattactttg tgaaatgatt tttcgatcaa ttattattat
      181 tcgttataca accgttttca acatattttg aaaataaaat gcactaaaaa actatcttta
      241 gcagaacatt tcattgtatt ttttggacca aacaataaaa aaagaagttt gaaaaaaatt
      301 caaacttctt tgttcgctta atctgtattt tagaagaaag gcactccctc tacttcgtaa
      361 gttccaagaa cagagcgcat ttgacctggt tcttcccaaa tatatccttc gcctaattct
      421 tctctttgat tcttttgttc agtttcaaag acaaacaacg gaacattttc ctctttaata
      481 tgtgtatttg tcaattccat caaactaatc ttgctaacat cgatatgaac tatttctttt
      541 aagaaattaa gtatattcgc taaaccagtc tttccttcag aaagttccgc taacgcaaat
      601 tccaacttgt catcaattga atggaccaaa aattttttag taccatcctc caaatgcaac
      661 ataattgtac cagcaactct tttgcttgcc aaaatttcca cctcgatcaa cttgttctat
      721 ggcttacata aaaattctag cacattttca gaaaaaatac actttttatt tcttattttc
      781 tttccgttct agttcagata atgcttcttc tatccaaata ggtaactgct ccgcaatagt
      841 ggtaaatccg atcttacgat ttttattcgt gaaatacctt tttctgcgat atggaacagc
      901 tggatttgtt ttttctaacg ttcttaaaga taaactgatt tttttggaat attcatcaat
      961 gtcgatgatc tgaactgtta ctggctgtcc gacttttaac aatgaatgga tatttttcgt
     1021 ataaccggat tgtacttctg aaacatgaat aagtccttgc gtctcgttat caagagaaac
     1081 gaatgcgcca taaggctgta tccctgtcac ttttccttca attatttgtc caattttata
     1141 agtcatctac ttccctctct tcgcatttat cattatactg taaatagctc aaaaaaagaa
     1201 gcgacgaaaa tgactggagg tcttaagatg atcgaatttg ataaacagat ttcaagaaaa
     1261 gatacagaaa gtgtaaaatg ggacgcaatt gctgagacat atcaaatgga tgacttactt
     1321 cctttgtggg tagcagatat ggacttcttg tctcccggtg gggtagctaa agcgtttagt
     1381 gaatacatca aacatggaat ctttggttat gccactctgt ctgataaact atatcaggct
     1441 atcattcatt gggaacgcca acaccatgct gtggagttga cgaaagaaaa tatcgttttt
     1501 acaagtggtg tgttaaccag tttagctgca gcagtacaaa cttttacaaa accaggcgat
     1561 tctattttga ttcatgaccc agtttatcct ccattttcct ctattataga aacaaatcaa
     1621 cgtcacatcg ttcgaagctc tttgctagaa aaaaataatc atttcatcat ggacttgtct
     1681 gacatggaga aaaaaatcga agaaaacaat gtaaaagcaa tgattttatg caatcctcac
     1741 aatcctggag ggcgtgtttg gagcaaagaa gaattgaaac agttaagcga attgtgttta
     1801 aaacatcaag tgattctgtt tagtgacgag atccatcaag atttaacttt attcgatcat
     1861 acatttactt ccatgctaac gattgatcct aatttagatg atctactagt tgcttttact
     1921 tccgcaacca agactttcaa tctagctgca atcaaaaatt caatggtatt tatcaaaaat
     1981 ccagagatga aagctgattt tgaaaaacat ttagtgatga accaacagca cgaaatcaac
     2041 acttttggac tgattggaac ccaagcggct tatgagacgg gtggagaatg gttaagccaa
     2101 ctgattcctt atctagaaaa aaatgttcaa acagttaatc agttttttga acagtatctt
     2161 ccgaaagtga gaataatgaa ccctgaagga acttacttaa tgtggttaga ttttaccgcc
     2221 tatatttcag acgaccaaga attggagaac actcttgttc ggaaaggaaa agtcgtatta
     2281 aatcctggca tcacgtttgg tccttccggt catgggcata tgcgcttgaa tattgcttgt
     2341 ccagaagaaa ctttactgga aggacttaat cgaatcaagt atgctttaga gacaatcgat
     2401 taaatcattt attcaaaata aaaggacgag accttactag gtttcgtcct ttttcataat
     2461 tactccgtac cataccaaac tattctgaaa tttcaattgt ttcaatcact acatcatgta
     2521 caggtcggtc ttgtggacca cgttgtacat ttgcgatttc atctactgtg tccatccctt
     2581 ctagtacatg tccaaagact gtatgtcgga aatctagcca aggagttccg ccacctttat
     2641 acgcttcaat gatttctgat gggaatccag caccttctag ttgtccaagc atattagctg
     2701 gcacatttgt gttatttaca ataaagaatt ggcttccgtt tgtgtttgga cctgaatttg
     2761 ccatagataa agctccgcga aggttgaaca gttcacgact gaattcatct tcaaaacttt
     2821 cgccataaat actttcgcca cccattcctg ttccagttgg atcgccgcct tggatcatga
     2881 aatcaggaat aacgcggtga aaaatcacgc catcataata accttttttg gaaagttcaa
     2941 tgaaattttg aaccgtcttc ggtgctagtt cagggaataa ttgaacagta atgtcaccac
     3001 ggtttgtttt gataacagct tttggtcctt tttcactttt taaatctagt tgtgggaatg
     3061 ccatattgtt tcctcctaat atttgcatac ttcataataa taataacata atacctaacc
     3121 tctcgtaaaa atactagatt tgttttcttt tatttttcat cactagatac attctataac
     3181 ttaccgtttc gatttcgaat tcaaacattt ttgagaagca ttctcaatta aattctgcta
     3241 tagtgtgaaa agggaggaat ttttatggaa atctatgaca ttacaatcat cggtgcaggt
     3301 cctgtaggga tgtttgccgc tttttacgca ggaatgcgcc aagctaaaac aaaaatcatt
     3361 gacagtctac ctcaacttgg tggtcaatta gcaactttgt atcccgaaaa atatatttac
     3421 gatattcctg ggtatccagc aataaaagca agtgaattaa tcgatcaatt agaaaaacaa
     3481 ttaactactt ttaaccatac attccattta aaagaagaag ttctctcgct tactcgtgaa
     3541 gacgaagtta tcgagattac tacaaataaa ggcatccatt attccaaagc agtcatttta
     3601 gctttaggaa atggttcatt ccagccaaga aaattgaatt tagataatgc tgaatcattt
     3661 gaaaaccacg gattggatta tttcgtgaat gatttgatga agtatgctgg aaaaaaagtc
     3721 gctattgctg gaggtggtga ttctgctatc gattggacgt tgatgttaga gcctattgca
     3781 agcgaagttt acttgattca tcgtcgcccg gaatttcgtg cgcacgaaca tagtgtatca
     3841 cgcttgaaat cttcatctgt caacttgctt actccctatc tcatcgatgg attatcaggc
     3901 aataatgggg aactgacaga tatccggtta aaaaaagtga aaagtgacga gacgattgat
     3961 ttgatgattg attctctgat cgtcaattat ggattctctt ctaatttaga acatctttct
     4021 tcttggggac ttgatagtac cagaaatgcc attactgtaa aatcagatat gtctacttct
     4081 attccaggcg tttatgcagc tggtgacatc tgtacctatg aaggaaaagt gaaactgatt
     4141 gctactgggc tcggtgaagc acctactgca gtgaacaacg ctttacacta tattaatcca
     4201 aaggaacgaa ctcagcctgg tcacagcaca agcttatacg ataaaaatct tcgtcctagt
     4261 tgatttttta tatctctttg catttcaagt taaacccgaa agcagtttct tgcatttttt
     4321 tcatgttccg ttaacatgaa atagagaaag aaaaaataaa gggggagcaa taatatggtc
     4381 gtcaaaactg agactttaga agaaaaggca agagaactat tgaaggaacg tggcgtaact
     4441 gtagaagata tcgcacaact tgtcatgttt ttacaaaaag actatatttc tgatttgact
     4501 ttggcggatt gtactgaaag tgtcaacagc gtgctaacaa agcgtgaagt acataacgcc
     4561 attatcactg gaatccaatt agatatatta gcagaaaaga aaaaattgat gagtccgttg
     4621 caacatatta tcgaagatga cgaaggcctt tacggtatcg atgagatcct agccttgtca
     4681 atcgtgaatg tttacggtac gatcggcttt acgaattatg gttatatcga caaggtgaaa
     4741 cctggtattt taaaagaact aaacagccac gatggcgttc atgtccatac cttcttagat
     4801 gatatcgtcg gagcgatcgc cgctgctgct gccagtcgat tagcacacaa agaaccagaa
     4861 aaaagaagtc gccttactca ataattcaac aaaaaaagaa agaaaacgtc tatttagacg
     4921 agttctttct tttttgtttg tttcaagcta ttttttccta agaaaagaaa aacagcaaaa
     4981 aataattcta ataatacaaa aaataagata aagctatgca tgaaaaattc ttctggaagt
     5041 acattgatga ttggatcagt aacgggatcg aataaccagt catcgttgtt gaaaaacgtt
     5101 tcatgaaaaa ggataaaaaa gcgatcaaat cctatcatca tgaaaaaacc gaaaacaact
     5161 ggcagtaaca tgccgatttg aaacggacga atcaaccgcc aaagccgatc attcttttgc
     5221 aaatatttga taaacaaaat actcgggatc aaagtgatga aaaacacagc ataatccact
     5281 aaaaacaaaa ttttcacttc ataaaagtga tgggctccgc tagcagaaac tgggaaatct
     5341 ggcaagctga ggatggtttt gaagggatta ttgagataat tcattaggtg atcaaaattt
     5401 ttcagtaacg tctcttgatc taaagaagta taatccaaaa tttgcaaatg tccgatatca
     5461 aatacgtata gtggtcgaaa gttgatcgtc acaccaactg ccagactgat cagtgtcaga
     5521 aataggctga acaatccagc atattccatc cactgccatc tcgaattttt catattagaa
     5581 gctccattca tccagtgatt gtttcacata ggtagctggg ataggcaacc caggtacatc
     5641 ttcttctttt gtaaaacctg ataaaacgag caaagtatct atattattgc gtatcccagc
     5701 ttggatatct gtttcatagt tatctccaac catgattact tcttcttttt gcagccctaa
     5761 tacttcaacg gctttatcca taatgatggc ttctggtttt ccgatgtaga tcggcggcgt
     5821 ttgggttgct gtctctacaa aagagatgac tgaccctgct ccaggaagaa ggccgcgttc
     5881 agtaggtata ttcttatctg ggttcgttcc aatgaaagta gcaccttttt gaatagcaag
     5941 tgttgcttta actacttttt cataggtcag ataattatct aagcctacca ctacatagtc
     6001 aggagcctct tcttcccatg taaatccggc agctaaaatc aagtcaatca atcctgcttc
     6061 tccgataaca tagacttttt tcccttttcc atctgctttc ataaaatcga ttgttgccaa
     6121 agtagctgtg tagactgtct ctggagctac atgaatgtca aactcatctg ctagacgatt
     6181 agctactgtt tctggtgact tagtcgtgtt attggtaaca aacaagaaag gtaattttct
     6241 tttttgcaat tcttccacaa atcgtttacc ggccggtatg ggttgtgtcc cacgataaat
     6301 cgttccgtct aaatcaatta aatatccttt atagtccatt catccactac tcttcccttt
     6361 ttctgatcgt aaatgctgtt tttgtttttt ctacttgttt aggttgttct tgttttttta
     6421 ctttttcaga ggtttttgct gactctgttt ttcgattttt aataaccggt tttttgcgat
     6481 tagatgcgac tggtcctttt ttctcatcga tatacgcacg attatggttt tttttctttt
     6541 ttcggcgctg agttgtcttt tcttttttac cgccgattct ctcgatgaca aaatacgcac
     6601 aaccaaaatt gcaatattcg taaagatagt cttccaagga atcgattcgc tgatctggat
     6661 gcgcccgtcg attatccgct tggaaaaatc cttttaggcg tagttgttca taaccccaat
     6721 ctcctacgat ataatcatat cgggctagta catcactgta acgttctcca agcttttctg
     6781 cattaaatcc ttcacgataa tcggacacaa gccgataatg tctttcacct atcaaaaaat
     6841 tactgtcatc taacagtgtc actagctcgc ctttgacttt ttctgcttta ggctcctcaa
     6901 cgatttcttc caaagcagct gtcagttctt cggtgactgt tgtttcctgt tcagctgatg
     6961 attttttctt agcaatttct gttttatgct caacgttctg ctgttcattt tgttttgcag
     7021 acatgcgttc accacctatc ttttttatta taccttgttt ttatttgatt gggtagtgag
     7081 ctttcaagta atctcctacc acacttcgga taaattcagg gaacaaaaat tcattttttc
     7141 cagcaatctc aatcgttgga aaaaatggaa caaacatcaa atgatctacg gtagtaaaag
     7201 tataattttt attcagatcg attggcttgt tcttccaaaa gacttgatgg ttaatagaat
     7261 catatgtgat tccattatag accatctgcc caaaaatctt accgcgaaat cccattccct
     7321 tcataggata gttacgtaaa aagttacgat ttttttctat ttctaaaact aagcggatca
     7381 gatcatttcc acccaatgtg acatttaaca agtgcatcgg atgcggcaac gcagtgtgaa
     7441 gctgatcctg attgacccgc ccttttggaa tttcagttaa aaacagacca ctgtttaata
     7501 ttgctgcttc cgtctttcct cgttctttca ccgcctcaaa ggctacttgg ataaacgaat
     7561 gctcattgaa gatatctaga ctcagagaaa atggcaaatc tgctacttct ttttctttta
     7621 ataattcatg cccacgttct agataactat ttatttcttg ttcatcttct gtaaaagctg
     7681 tcattgtttc ggtagggatg gctttggctg aatgcttaag gatttttttc tcttcatcga
     7741 tggttaaatg gacttcaccg acatactgcc cgtactttcc agcggctgct atttggacat
     7801 cattgattac gtgaccttct ctgaataaat gatgggtgtg cgatcctaaa ataacatcaa
     7861 ttgctggtat ttcttttgca atttggaaat catcctgaat cccgagatga ctcatcaaaa
     7921 ctaaaacatc cacttgtgaa cgtaaatctt cgactaattc tggcaaaata tcataaggat
     7981 tccgtatgtc ccagccattc ggattataag taagcggaaa tggagcagta aatgcaatta
     8041 gtccgatttt cgtctggtgt ttcgttgtaa tgattttata tggtttcgtc catgccggtg
     8101 gctggagcgt atttttatcg aataagttat ccaggatgat atcaaactca gcatgatcgt
     8161 ataaatgatt caactcttct ttggaatttc ctacgccttc attgtttcct atcgtcgctg
     8221 catcataacc aacttcattc atcagcaaga tattttcctg tccattcgtt gcttctgaca
     8281 atggatgcca acgatccaca aaatcaccta gatctactgc aatcgttgtc gttttttctt
     8341 tcttttcgtt ttctcgttta cgttgatcca aaaaccttct gatccttggc caattttcta
     8401 aatgagaatg cagatcattc gtatgtaaaa tcactatttt ttccatgtac cctctcgcct
     8461 ctctttatct ttatcatacc ataggcagct ttttcttcca ttgaaacaag ctctatttca
     8521 gagtttttcc tgccttgaag caaattgtat aagtacaata ttcgataaat cagtctggct
     8581 aaaaaatcag gaacattcat ttttacagta ctataaaaat aaaaaaaaca gacccgaaag
     8641 tctgttgtcg tttcattata atcagacaca tggcggaacc tgcttagtgc tgcttccttc
     8701 cagacctgac acgcttcaca agcccaccat tgtcctagcc tttcggcaaa gaatagtata
     8761 ccttattttt cattcgttga ccagtctttt tttaatttct tttttcgctg gcggagcttt
     8821 cggaaaaagt cagtcaaaat catcccacat tcgtcttcta aaatgccacc ttctacatat
     8881 gcttggtggt taaaccgctc gtcctccaaa agattcatca aggtaccggc tgttccgcct
     8941 tttggatcat acgcgccgaa ataaacttct gctacacgag acaaaatcat cgcgccgctg
     9001 cacataggac aaggctctaa ggtaacaaaa agtgcagcat cttccaagcg ccagcttccc
     9061 actttttcac aggcttcctt gattgcaagc atttctgcat gtgtagtagc atcttgcgtg
     9121 gtttctctaa ggttatatcc tcttccaatc acttttccat ccaatacaac cactgctcca
     9181 atcggaactt catgaagcat ttctgctttc tttgcttctg ccaatgcaag gcgcatccat
     9241 ttttcttttt ctactgcatc aagatttgta tttaccaatt acccttccct gcctttgttt
     9301 tttcttgttt tactttagca tatgataaaa aggctgtaac taactttttt ttctatggta
     9361 aaataaatac aagaggtgaa caagatgaaa atggataaat tacttgctct ttacccacaa
     9421 gcacagaaaa aaaactctcc agcatcagaa gcggatatcc tttcttttgc aattgatgat
     9481 gcttttatct ggatcaaaca agattcatta tctcaacaag aaaccagcct tttgaaagcg
     9541 ttattccctg taatcaacga tcaaaaaaaa cacccatggt atcgctatct ttttaaagat
     9601 gcttcttgtg ttcaagaaaa aagctttcgt ttgatccagc ttcacgtcga atcaaaagga
     9661 gaattcttaa aaaaagaatg gcaagatact attttggaat tatttcgcta ttctgctgat
     9721 ttcttttttt atacagacac agatgcgctc ttagtcgaac aaataaaccg tgattatctt
     9781 gatgctactg aaatcaacgg tatttttcta tctttagatg ctgattttga tacgacaacg
     9841 tctgtattcg ttggttcttt ccattcacct ggcaccgacc tcgtcccttt gtttgctgaa
     9901 gaaagaaaaa tttttttaac agagcaaaac aatctttata ctcacagccg tactttttct
     9961 atgcaagatg tggctttaca ttactataca aaagatactg taaaagatag taagttgatc
    10021 aaaagttaca tagaactgat caaacaaaat gaaatggaag tgatcattca agaattatgg
    10081 aatgaattag gcaatgtcag ctctgctgca aaaacactgt ttcttcatcg taatactcta
    10141 aaatatcgaa tcgaaaaatt tcaagaacag tctggtttca atctaaaaaa agccaacgat
    10201 ttactgtttt gctatctttt attgcttcat gaacataatt aaagatagat taaaggggtt
    10261 gtgacaataa ttttgtcaca accccttatt tcgaatgaat agtgttcaaa agctagtttc
    10321 tcttacatca ttttttcaac aaaatgcatt agccggtctt ttacatcttc ggttgaatct
    10381 ttgattgaat caccataaac agagattttc ttttttcctg atttgatttt tgctaacatt
    10441 cccatcaaag aatccaagtc actatctgaa agaccatcta cgatttctag taatttttca
    10501 tttccatcaa atttgtcata gacgaatttt tttactttcg tccgagtagt catatgatgg
    10561 atcttttcca ttacttttcc cgatacaacg actgctgtcg ctacactggc agcggctgct
    10621 acgcttaatc cgattgttac ttttgttgat gttttcataa gtatcagctc ctttttatct
    10681 atctccatca tagcatagac aaaaaaataa acgaactaat ccactcagtg aaatctcaat
    10741 cctttaggga aaaagttctt tactgttcct tttacttgtt tcccaaatcc gacaggtatc
    10801 ttatctaata ctagcaaaac ccatccttga ttcccatcac gttgaaatgt ttctcctgct
    10861 gtgtaagatt gccattcctt ttgagtgatc ggcaagcaag gtatattctc tatctttttt
    10921 gtagcaagcg ctaatgcata gctaggttca aatcgattct ttttgaaatc tcctaaatgc
    10981 aaacctgtac gtacgacttt taaaccatcg agagaagggg ctaactcagg aacttcccaa
    11041 agatggtcgt tgaacactaa aagtcttcca gtagcttcat aatgaaaatc atttgaaaac
    11101 tccgtccaga gtttttcctg ctcttttgtc atttgcactt tacttttttt tcgtgtcttc
    11161 ttttctttat gcatctgatt ttgtccatga aaagtcagct ttgcaacaaa atgtccttca
    11221 ccctgatctt tatgaggcca tatacgtatt gttttttcca aaccagctac gcttccccat
    11281 tcagaacgtc ctgaactaac ggattgagtc aaaggaatct cctcaatcgt tactggataa
    11341 ttttctacaa gccagctaat gatctcttca ttttcttctg gtgcaaacgt acaagtcgaa
    11401 taaatcagtt gtcctttatt ttttagcatt tttattgctg aagacaaaat ctcttgctga
    11461 cgtttttgac agtatagcgg agattcttct gtccactctt ttattgcatt tggatctttt
    11521 cggaacattc cttctcctga acaaggagca tcaaccacga tccgatcaaa aaagcccgaa
    11581 aaatgaggga caagttctgc gggtgcatga tttgtcacta ttgcgttcga aactccccat
    11641 cgttctatat tttctgataa aatcttcgca cgtttcggaa agatctcatt cgtcactaat
    11701 aaccctttcc ccttcatttg agcagctaat tgggttgatt taccaccggg agccgcacac
    11761 aaatccaaaa ctttttcacc gggttttgct gcagcagctg ttccaacgat cattgcactt
    11821 ggttcttggc tatattcata cccagcttga tggaggaaag actttccatt gacagtgcca
    11881 agaaatccct cattgctgta gggagcgggt tgtaattctt cgctatggta agtttggacc
    11941 atgtccaatc ctgccggttt tagtggattc caccgaaatc cttttttcac gcttccttgt
    12001 tcaagcgcag aaaaaaaatc ggatgcttct tcgcctaaaa gaagacgata ttttttgata
    12061 aattgctgtg gtaacgttgt cgcttcctcc ttcattccaa tgtttcgtta gacattttac
    12121 aatctttttt acgaaaaata aaggtggctg atctactttt tcatgttgct gcctaaggtg
    12181 actgttcctt taagatattt tccaagtatt catccgtcac ctcgttttta tcccaaacag
    12241 cgttcatcat ttcaagaaaa tcttgagaag aatcatctga gcgcaaactt cccaatgcat
    12301 taagccatac caattcaagc gacttttctt ctttgatctt cgtgtgacct ttgacatatg
    12361 agaaaaatgg ccgcttaaat agcgaatcca tgtctaaatc agattttgca atgatttcac
    12421 gaatactttc atggatagaa aaaaaatctt tcaataagta gacaatgctt aaaacatcat
    12481 cggaactgca ttcagctaag tagttttcaa aataaacagt gatttcttct aatatcttct
    12541 cttttaaccc gctgattgat ttgaaatgtg tataaatcgg gtatgtagaa caaccgcaga
    12601 actctgctaa ctctcttgca gtaattgact tcattccata ttcttccacg tataccatac
    12661 ttttgaacag caaatattct ttactataat ttgttactct ccccatcatt cttacctccg
    12721 tctcttttca tctacccata aataaataac ataaataatt ctaaaaatat tattgataat
    12781 tatcaaaaca tatttagtta tatccattaa aaatcaattt ttccgcctta aaaacaagaa
    12841 tatcttgaca aaatacccta ggaaataaac aatagaataa acaaggtaac aaaaaaatta
    12901 aataacgtta tataaaaaag aaggcgacac tctgtgagaa taacacagac tgaaatgact
    12961 ttccactcac agtttttgtc taactatata caaaaacaga ataaaaagga ggtatttatg
    13021 ctaattgaag caagaaaaaa agagttactt gttctacaaa tggtcattca aaaaaataat
    13081 tgttctttac aagaattatc caatgactta catattccaa aacggacaat caaagaactg
    13141 atccgaaaaa taaatgtatc actcaagcaa tttttattga ttgatgaatt tatttattct
    13201 aatcataaag gagagatcca aaccaaagaa accgatcaaa ctaaaaagtt agctgttttt
    13261 tctaagttaa aattatgcta cctaaaagaa tccaatcgtt ttaactattt gatgttacta
    13321 atcaattatc cacaaactgc tgtaccaaaa aaatatttac ttgaacaact ttatatatcg
    13381 ccctcctatc tcgaaaaact gacaaaacag ctcaatcatt ccttaaaaaa attccattta
    13441 cggatcctat ctgtaaaaaa ttgctgtact cttgaaggaa atgagttatc tatccgtctc
    13501 tatctttact tttttctttc agatacattt ggaggtaacg attggccatt ttcctcactt
    13561 gaattaaatg cactaaaaat gaacagtaca gatgagcaaa tagaaaaaat ttcaaaacaa
    13621 gaaaaaaata tctacttgct gttaacttta ttcatattac gaaacaaaca acaggcatat
    13681 tttccaaaaa cttcttctga aatcactgaa ttgatggact tattgcagca tacacatgat
    13741 tattcagaat actttacttc tttttcagtg agtcgccctt gtgaagagac agaaacagag
    13801 agagcctact ttaattattt tttctattgt ttttttccaa atcaaatctc ttttcgacaa
    13861 aaacaggaga ttggtttttt attttccaca agcaaaaacc ttatttgtcg acaagtcggt
    13921 caattgatga cttacttaac tcaacagttt cctctcaacc atttagaaaa taaaatacca
    13981 atttattttt attttcttgt gttggcagtt gtttttttcg aactgttggg tgaatccagc
    14041 agtagttttt ctacactttg ctttccagaa gccacgtcca atcattctag ttcttttata
    14101 caggaaaaga aattaagaga aaaaatcgat acaatttttt caaaaaatgg ggaaagaaac
    14161 caaatcagtc aataccttag tcaattgatc caacacttac tacaaacaga aacgaaagag
    14221 cagttactca tccatttgca gataaccaaa cagtttacag gaaattatca tcttcaaagc
    14281 catttgcagc agttatttcg caaagatgct gttaaaatta ccgcatattc agaacatgct
    14341 gatttgatca ttacagatca gcatgagaaa atttcagatg aaacggagac tttctatctg
    14401 gatacaacgc acaatacagt gttatttggc aagttaataa aagcggttca tgaactgtat
    14461 atgaaaaaag gttatgagaa caaaacaaag aggatgtgac aaaattcttg tcacatcctc
    14521 ttatt
//
LOCUS       DACDXO010000065        14392 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E23605_2017
            SAMN12639989-rid9881463.denovo.066, whole genome shotgun sequence.
ACCESSION   DACDXO010000065 DACDXO010000000
VERSION     DACDXO010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639989
            Sequence Read Archive: SRR10023389
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741914.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:42:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,972
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 33 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 17 of 73
            Pseudo Genes (multiple problems)  :: 10 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14392
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639989-rid9881463.denovo.066"
                     /isolate="E23605_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            4..552
                     /gene="hslV"
                     /locus_tag="IWM35_002218"
     CDS             4..552
                     /gene="hslV"
                     /locus_tag="IWM35_002218"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase proteolytic subunit"
                     /protein_id="HAP9920057.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            566..1966
                     /gene="hslU"
                     /locus_tag="IWM35_002219"
     CDS             566..1966
                     /gene="hslU"
                     /locus_tag="IWM35_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HAP9920058.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            1984..2775
                     /gene="codY"
                     /locus_tag="IWM35_002220"
     CDS             1984..2775
                     /gene="codY"
                     /locus_tag="IWM35_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HAP9920059.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            2857..3732
                     /locus_tag="IWM35_002221"
     CDS             2857..3732
                     /locus_tag="IWM35_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HAP9920060.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(3864..4502)
                     /gene="plsY"
                     /locus_tag="IWM35_002222"
     CDS             complement(3864..4502)
                     /gene="plsY"
                     /locus_tag="IWM35_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HAP9920061.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            4629..5063
                     /locus_tag="IWM35_002223"
     CDS             4629..5063
                     /locus_tag="IWM35_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HAP9920062.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            5229..7274
                     /gene="parE"
                     /locus_tag="IWM35_002224"
     CDS             5229..7274
                     /gene="parE"
                     /locus_tag="IWM35_002224"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HAP9920063.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            7286..9736
                     /gene="parC"
                     /locus_tag="IWM35_002225"
     CDS             7286..9736
                     /gene="parC"
                     /locus_tag="IWM35_002225"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HAP9920064.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDISIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            10013..12244
                     /gene="pflB"
                     /locus_tag="IWM35_002226"
     CDS             10013..12244
                     /gene="pflB"
                     /locus_tag="IWM35_002226"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HAP9920065.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            12488..13249
                     /gene="pflA"
                     /locus_tag="IWM35_002227"
     CDS             12488..13249
                     /gene="pflA"
                     /locus_tag="IWM35_002227"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HAP9920066.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            13334..14260
                     /locus_tag="IWM35_002228"
     CDS             13334..14260
                     /locus_tag="IWM35_002228"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HAP9920067.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
BASE COUNT         5049 a         2494 c         3124 g         3725 t
ORIGIN      
        1 aacatggtcg aatcaccatt tcattcaacc actatctgtg ccgtcgaaaa agacgggaaa
       61 tttgctatgg ctggagatgg ccaagtaaca atgggtgaat ctgttgtcat gaaaggcact
      121 gcaaagaaag tacgacgtat ctacaatgga gaagttgttg taggtttcgc cggaagtgtt
      181 gcagatgcgt ttactttaga agaaaaattt gaaggcaaac taaacgaata taacgggaat
      241 ctacaacgcg cagctgtcga attagctcaa gaatggcgga cacagcaatc tatgcaaaaa
      301 cttgaagcga tgctgatcgt aatgaatgat aaagaaatgc ttcttgtttc aggtacagga
      361 gaagttatca caccagatga tggtatctta gctatcggat caggagggaa ttttgcgttg
      421 gctgcagccc gagcaatgaa aaactatgct caaaacgaca tgagtgcgaa agaaattgca
      481 gaaaatgcgt taaatattgc cgcagatatc tgtatcttta ctaatcataa tattatcgta
      541 gaagaactgt aagaggtgag tagatatgta tgaattaaaa caaacaccaa gacaaatcgt
      601 aagtgaatta gatgaatata tcgtaggaca gcacgcagca aaaaaatctg ttgccgttgc
      661 tttgcgtaac cgctatcgcc gtttgcagtt agatgaaaaa atgcaacaag atattacacc
      721 aaaaaatatg ctgatgatcg gacctacagg ggtcggtaaa acagaaatcg cacgacgttt
      781 ggcaaaaatc gtcaatgcac catttgtcaa agtcgaagca acaaaattta ctgaagtcgg
      841 ttatgtcgga agagatgtag aatcaatggt tcgggattta gtagaaaatg cgatccaaat
      901 cgtagaaaaa gagcagtaca gccgtgtata cacaagtgct aaaaaaaatg cagaaaaacg
      961 tttggtaaaa atcttggtac ctggtatcaa aaaagaacag aaacaatcca ctaatcaatt
     1021 tgaacaaatg atgaatcttt ttaatgctag tcagcaaaat accgctgaac cgcaagaaga
     1081 agtgacagaa gatattcgaa tcaatcgccg tacagtcttg gaacaactac aaaagggttt
     1141 actggatagt cgtgaagtaa ccattgaagt tgaagaccaa aagaaacctg caccaatgat
     1201 gaacaatgga ttggagcaaa tggggatcga cctaaatgaa actctaggtg ctttgacacc
     1261 gaagaaaaaa gtggaacgga cggtaacagt caaagaagct ttagagttat tgatcaagga
     1321 agaatcagca aaattagtca aggatgctga tatccatagt gaggcaatca aattagcgga
     1381 agaaagtgga attattttta tcgatgaaat cgataagatc acttctaaat cacagcaatc
     1441 tggagaagta tccagagaag gcgtccaaag agacatattg ccaattgtcg aaggttcaca
     1501 agtaaacaca aaatacggga tcatccaaac agatcacgtc ctattcattg cttcaggagc
     1561 tttccatttg tctaaaccaa gtgatctaat tccagaactt caaggacgct tcccgatacg
     1621 ggtagaatta gatgatttga cagctgaaga ttttgtgaag attctgaccg aaccaaataa
     1681 tgctttagta aaacaatacg tcgcgttgat cggtacagaa aatgtgtcag ttacgtttac
     1741 aaaagaagca attgaacgga tcgctcaaat cgctttcgat gtcaaccgtg acacagataa
     1801 tatcggcgca cgcagactgc ataccatttt ggaaaaacta ctagaagatc tattatttga
     1861 agcaccagat atgcaaatgg gagaaataac gatcacagaa gcatatgtgg atgacaaact
     1921 tggctcaatt gcggcaaatg aagatttaag tcgctatatt ttataaaaaa tcggaggacg
     1981 acaatgacag acttgctaac gaaaacccgc aagataaaca cacttttgca gcaaaagaat
     2041 acttttgaca tgcaagcaga tttgccttac gataaaatgg ctgtcacact aggtggtatt
     2101 cttgacagca acacctatat cataggaagt gacggagtat tactaggtta taatgaacgt
     2161 cacgatgtga acaatgaacg aatcaaaaca atgtttgttg agaaacaatt tcctgaatct
     2221 tatactgata cagtggcaca gttgatgaaa actgaagcta atatcccaat tactagtgat
     2281 attactgcat ttccggtaga gtcaagagag aaatatccat ttggtctgac gactgtcgtt
     2341 ccgatctttg gagctggcga acgtttgggt acgattatct tatctcgtat ggaacaatcg
     2401 tttgcagatg acgatctagt cttagcagaa tatggagcta cggttgtcgg tatgcagatc
     2461 ctttatcaga agtctcgaag catcgaagca gatgtacgga gtgcaacggc tgtccaaatg
     2521 gcaatcaaca cattatctta tagcgaattg aaggctgtac aagctatctt tgaagcattg
     2581 gatggagatg aaggtcgtct gactgcttct aatattgccg atagtatcgg catcactcgt
     2641 tctgtgatcg tcaatgcttt acgaaaattg gaatcagctg gaatcattga atctcgttcg
     2701 ttaggcatga agggaactta tttaaaagta ttgaattccc gttttaaaga cgagttagca
     2761 aaacacagtt attagaagta aggggactgg ggcagataga aatgtttgtt ccagccttcc
     2821 cattttaaaa agtaagtcga agttggagga atggcaatga ctgttattat tcaaaatgac
     2881 cagctgatcg ccgaaatcgc tgaacacggt gcggaattga tcagtctgaa atcaaaagaa
     2941 catggtttag agtatatctg gcaaggtgat ccagcgtatt ggggcagaca tgcaccggtt
     3001 ttattccctt ttgttggacg tttaaaagat gatcagtatg tttatcaagg gaaaacatat
     3061 caaatggggc aacacggatt tgctcgggat atggattttg aagtcattga acatggacaa
     3121 gaaatggcta gtttcatctt aaggagtacg ccggagacat tagaaaagta tccatttgat
     3181 tttgaattag tgatcagcta tgaattagga ggagacggca tcacggttca ttatcaagtg
     3241 gcaaataccg gtgaaaaaga aatgttcttt tctatcggtg gacatcctgc tttcaatgta
     3301 cctcttgaag aaggattgtc atttgaagat ttctatctgg cattttctcc attgaaatca
     3361 agattacgcc taccattgaa aggcccattc atcgatatgg atcaaaagac gttaggtcag
     3421 acaaatacga atctggcttt gaatcgtgaa ctttttgagc aagatgcctt ggtttttgaa
     3481 acaaggggtc taaatgcgta cgccattcga agtgaaaaat cttcccatag cgtaacttta
     3541 agctataaag atatgcctta tgtagggatc tggtctcctt atccaaaaga agcgcctttc
     3601 gtttgtatcg aaccgtggtg cggaatcgct gacacggtga acagtagtgg aaacttgatg
     3661 gaaaaagtag ggatcaatca actaggtgcc catgaaatct tcaagacaaa atattccatc
     3721 acagtgaagt aagaggttgt gaccgagaag ttagcaggga acaccgttta ccaaggtgtg
     3781 taaaaaagct atcctgtaag taataaggga gtgtgacatc gatttgtcac actcccgttt
     3841 tatctctttg gaatgaaaaa gtatcaggat tttttcttgc tgctattcaa accaaacggg
     3901 attcgacttt cagtatggtt tctgatacgc ttgatattgt ctttatgccg ataaaagata
     3961 aaggaagaaa ctaaaaaggc aatgatcgtc agtaaccaat tgggcttagc caaaatcaca
     4021 ggagctacat aaggaaggac gatcgtagat aaagtgatca atactgcact gatcatactg
     4081 ctcaaactca ccatacttgt acaaaataaa ctaacgataa agatgataca agaataaaca
     4141 aaaaatacag gattaaatcc taaaagcatt cctgcacttg tcgcaacagc ttttcctcct
     4201 ttgaacccag caaaaatcgg aaatgtatgg ccaagaatgg cgcatacccc aaaccaaaga
     4261 gggttaatcc cagaaaggtg gaagatcatc ggcaaacaag tagccaatgt accttttaaa
     4321 atatccataa gcaggacaac aatcccggct gttttgccta atacacgaaa tgtattggtc
     4381 gttccagtat taccgctccc gtgttgtcgg atatcttttt tgaaaaacaa tttgccaatc
     4441 cagacaccgg aaggaataga acctaaaaga tatgctaaaa taagtaataa gatcaatttc
     4501 attttttacc ctctttcaag ttgaaaacta tcctttattt tagcatgaat aggtaacagg
     4561 aacaattgaa aaaatgaaag atacaagtat aatagatgga ataaaaagaa aagcaagggg
     4621 tggcagaaat gtctgtaaaa aatccaacac aagaaacgat tttcaactat ttggaagaag
     4681 caaaaaatat tgccgtcgta ggcttgagca ataaaccaga acgaacaagt tacatggttg
     4741 cacaggtatt gcaagacaat ggatataaaa tcataccagt aaatccatta ttggctggtg
     4801 aagaaattct aggcgagaaa gtctatgcga atttgacaga aatcgatgat ccaatcgata
     4861 tcgtagatgt atttagacgg agtgaatttt tacctgaaat agcaggtgat tttttgaaaa
     4921 ctgatgctaa agtgttctgg gcacaattag ggattgaaaa caaagaagca gctgagatgc
     4981 taagaaaagc aggacgtaca gatatcgtaa tggatcgttg tattaagatt gaacttggta
     5041 agttgaaaga aaaccaaaaa taaaaatgac cacaaagaag agatgatcga tagaggggaa
     5101 tgtcgaaaaa gcggttagga gataattaaa attgatttct cgtctaccct cttttttttc
     5161 acgacctttt ctagtatgat aatcgtggtg cggttttgcc gcaaagtatt attgaaggag
     5221 cgtttgcctt ggcaaaaaaa gtaaacaacg aatacaatga ctcgtccatc caagtattgg
     5281 aaggattaga ggcagttaga aaaagaccag ggatgtacat tggatccact gacagccgag
     5341 gcttgcatca tttagtctat gaaatcgtcg ataatgcagt tgacgaagca ttatcaggtt
     5401 atggcagtga gattgatgtg accatccatg aagacaatag tataactgtt gcagatagcg
     5461 gacgtgggat gcctgtagga atgcatgctt ccggaatacc aacagttgaa gtgattttta
     5521 ctgtgctgca tgctggcgga aagtttggtc aaggagggta caaaacttct ggtggactgc
     5581 acggtgtagg agccagtgtg gtaaatgcct tatcgaaatg gctaattgta acgatcgtta
     5641 gagatggcgt agagtatcag cagaaattta aaaacggcgg taaacctgac ggtactttga
     5701 aaaagatagg aaaaacaaaa aaagcaaacg ggacgactgt ccatttttta ccagatgata
     5761 caatattcag tacaacgaaa ttttcatatg aaattttagc cgaacggtta agagaatctg
     5821 cctttcttct aaaaggcgtt aaaatctctc tttctgatct tcgtggagaa gaaccagtca
     5881 aggaaatttt tcattacgaa gaaggaatca aagaatttgt tgattatctg aatgaagaaa
     5941 aagatacatt gacaccagtt gtttattttt caggagaaaa agaaggtatc gaagtagagg
     6001 tcgcttatca atacaatgac ggttattcag aaaatgtttt atcttttgtc aataacgttc
     6061 ggacaaaaga tgggggaacc catgaagctg gtatgaaagc ggcgatgacg aaatcttata
     6121 atgaatatgc gagaaaagtt ggtttgctga aagaacggga caagaatctg gaaggtagtg
     6181 atttcagaga agggttagct gctgtacttt ctatccgtgt acctgaaaat cttctgcaat
     6241 ttgaagggca aacaaaagaa aaacttggta cgccagttgc tcgtaccgta gtagataacg
     6301 tcatcagtga acaaatgggc ttttatttgc aggaaaatag cgaaatgagc cagatgttgg
     6361 tccgtaaagc aatcaaagcc agagaagcga gagaagcagc acgtaaagcc agagaagaaa
     6421 gccgaaatgg gaagaagcgt aaaaaaggcg aatctcttct ttcaggaaaa ttgacacccg
     6481 cacaatcccg aaaccctaag aaaaatgaat tgtatttagt cgaaggggat tctgctggcg
     6541 gatctgctaa acaaggaaga gacagaaagt tccaagcaat tctaccttta cgaggaaaag
     6601 tcatcaatac cgaaaaagct aaaatgcaag atatcttgaa aaatgaagaa atcaatacga
     6661 tgatctatac gattggtgca ggagtcggtc ctgagttttc tatcgaagac tgtaattacg
     6721 acaaagtaat catcatgacc gatgcggata cagacggcgc ccatattcaa gtattactgc
     6781 ttactttctt ttatcgttat atgaaaccgc tgattgaagc tgggaaagta tatatcgcgt
     6841 taccgccttt atataaagta tccaaaggac aaggaaagaa gcaagtcatt gaatatgcgt
     6901 ggacagatga cgaattggca gcaatgatta aaaaagtcgg caaaggatat atgctgcagc
     6961 gatataaagg acttggagag atgaatgcgg aacaattgtg ggaaacaacg atggatccga
     7021 cttcccgtac gttgatccgc gtccgtatcg atgatgctgc gcaagctgaa cgtcgagtaa
     7081 ccacactgat gggtgacaaa gtagaacctc gaagaaaatg gatcgaaaat cacgtgcaat
     7141 ttacgctcga agaagatggc agtatcttag ataaaaaaga ggatactgaa atctctcctt
     7201 cagtttctaa tgatttatta gatgaagaac gagcagataa aaatgaaaac aatcagttat
     7261 ttgaagtaga ataggaggtc acctgatgga aaatcgacaa gaagtacaag aattaacttt
     7321 agaagaagtg atgggtgacc gcttcggacg ttattccaaa tatattatcc aagaacgtgc
     7381 actgcctgat atccgtgatg gattgaagcc agtgcaacgc cggatattat ttgccatgaa
     7441 caaagatggc aatacgtatg acaaaggatt ccgtaaatca gcaaaatctg tcggaaatat
     7501 catggggaat tatcatcctc atggtgacat cagtatttat gaagcgatgg tccgcatgag
     7561 ccaagactgg aagttgcggg aagtactgat cgaaatgcac gggaataacg ggagtatgga
     7621 cggtgatcca cctgctgcga tgcgttatac tgaagccaga ttatccaagt taagtggaga
     7681 gatgctagca gatatcgaga aagaaacagt cgatctcgtg tggaattttg atgatacaga
     7741 aaaagaacca acagtccttc cagctcgata cccaaacctt ctcgtcaatg gatcgacagg
     7801 tatatctgct ggatatgcaa cagaaatccc gactcataac ttagctgaag tgatcgatgg
     7861 cacaatctat atgatcgatc atccacaagc gagccttgaa aaattgatgg aatatattcc
     7921 tggacctgac tttccaacag gagggatctt gcaaggaaaa gatgaaatca aaaaagctta
     7981 tgaaacagga cgaggaaaag ttattcttcg ttcgaaaaca aagatagagg caatgaaggg
     8041 taataaacag caaatcgtga tcacagaaat cccatatgaa gtaaataaag cgaccttggt
     8101 gaaaaaaatg gatgagatcc gtttgaataa gaaaatcgat ggaatagctg aagtacgaga
     8161 tgaaagtgat agaacaggtc tgcaaattgt cgtcgaattg aaaaaagacg ccaatgctca
     8221 aggaattttg aattatttgt tcaaaaacac tgagctacag atcaattaca atttcaatat
     8281 ggtcgctatc gaccatatga ctccgcatca agttggactg aaagacattt taagaagtta
     8341 tatcgagcat cgaaagcaag tcataacaaa acgtagtcaa ttcgatttag ccaaagctca
     8401 aaaaagacag catatcgtag aaggattgat gaaagcttta tcgattctgg atgaagtaat
     8461 cgcaacgatt cgtgaaagta aagacaaaaa agacgccaaa aagaatttag tcgacgtctt
     8521 tcaatttaca gaagaacaag cagaagcaat tgtcacattg caattatacc gtttgacgaa
     8581 cacggatatc acagagcttc aaaaggaatc cgaaagcttg attgcgcaaa tcacagagtt
     8641 gaataaaatc ctttcaaatg acaaagagct tttctctgtt atgaaaaaag aattgcgaga
     8701 agtgaaaaaa aattattctt ccgctcgtct aacgacaatc gaagacgaaa tcgaagaaat
     8761 caaaattgat acgcaagtct tagtggcaca agaagacgta attgtcagtg tgacgcgaga
     8821 aggctatgtg aagcgaacaa gccttcgttc ttatagtgct tccaaaccag aagaaatcgg
     8881 gatgcgagaa ggcgactact tgctatactc aggtgaattg agtacattgg accatgttct
     8941 tctgatcaca aataaagcaa atgtgatcta ccgccctgtc catgaactac ccgatttgaa
     9001 atggaaagat gctggtgagc acatttctca aacaatctcc aatctgtctg tagacgaatc
     9061 gattttagct gttttcccat atcaaaaaat tcaagcagag aaaacatttg tattcatcag
     9121 caagaatgga ttgatcaagc aaacacgaat gacagagttc gaaccatggc gcacctataa
     9181 gagccgtccg ctcagctgta tgaagctaaa ggcggaagaa gatgaactga ttgctgtgta
     9241 tctgaaagaa gatcagccag gcttagatgt tttcttagta acgcatcaag gaatgggttt
     9301 gcgctatcct ttaacagatg ttcccgttgt tggagcaaag gcagctggag tgaagtctat
     9361 gaatttgaaa gatggagata ggattatcaa cgggctgctt gtgttagcag aaggtgatac
     9421 gccgatcgta atcgttactc agcgaggagc tgttaaacgc atgcttgctc aagaaatcag
     9481 ccaaacttca cgtgcgaagc ggggagtgac ggtcatgcgt gagctgaaaa aaaatccgca
     9541 ccgtattatc tatatgtcgg aaggaagatc aaaagaaata accttgatga atcaaaaagg
     9601 gcagcagtta acgattgacc caactgattt ccctataggt gatcgaacat cgaatggatc
     9661 atttgcgatg gatgagaaaa aaggtggaga agtcatcgaa gttatcgatg ctcctgagat
     9721 tttcatcgag tcgtaactgt tgtagtattt taatgaagag acgccatctg tattaataca
     9781 ggtggcgtct ctttttcatt catttttaat aaatccttat acgtttgcaa aaaaagatga
     9841 aagtgttcac aatgcttaaa tgttggtatg tcaacgttga tttaaatata tgtaaataaa
     9901 acataaaaat tgttaaataa aaaacaagaa aacccttgca aagatagggt gctgtgttat
     9961 actaatcacg taaaaactgt tacataaaac agaaatttag gaggtccata caatggagca
    10021 atggaatgga tttaaaggta ccaaatggaa agaaggcgta gacactagag actttattca
    10081 aaataactac actgaatatc gcggtgatga cagctttttg gaaggcccaa cagaagcaac
    10141 agaaaaatta tgggatgaat tgcaaaagtt aaatgatatc caattcgagc gtaacggtgt
    10201 ctatgatatg gacacagaaa tcgtatcaac tattacttca cacgatgcag gctatttgga
    10261 taaagatttg gaaaaaatcg tcggtctaca gacagacaaa cctttgaaac aagcattcat
    10321 gccgttcggc ggaatcaata tggctaacaa tgcgctaaaa agcaatggct atacaccaaa
    10381 cgatgaattg acacatattt tcactgactg gcgcaaaaca cataatcaag gtgtatttga
    10441 cgcttatact gctgaaatgc gtgctgctcg taaaaacaaa atcattacag ggcttccaga
    10501 cgcttacggt cgcggacgta tcatcggtga ctatcgtcgt gtggcattgt atggaatcga
    10561 ctatttgatg gaacaaaaga aaaaagatca tgatatgact ggttatcata caatgagtga
    10621 agaagtgatt cgtttacgtg aagaaatcac tgaacaatat cgtgcattag cgcaaatgaa
    10681 agaaatggct gcgaaatatg gctatgatat ttctaaacca gctgcaaatg caaaagaagc
    10741 tgtccaatgg ctatacttcg gatatttagc agcaatcaaa tcacaaaatg gtgctgcgat
    10801 gtcaatcgga cgtatctcag cattccttga tatctatatc caacgtgatt tggataatgg
    10861 agtaatcaca gaaaaagaag cacaagaatt gatcgaccac ttagtaatga aattacgtat
    10921 ggtcaaattc gcgcgtacac ctgaatataa ccaattattc tcaggattcc ctatctgggc
    10981 tactttatcc attgcaggta tgggattaga tggtcgttca ttagttacaa agaatgattt
    11041 ccgtctgctt catacattaa caaatatggg accttcacca gaaccaaact tgactgtttt
    11101 gtattctgaa caccttccag aaggattcag aacatatgct agcagaatcg ctatcgaaag
    11161 ttcttcgatt caattcgaaa atgataatca gcttcgtgaa caatggggtt ctgatgattg
    11221 tgcgattgct tgctgtgtat caggaacagt tgtcggaaaa gacatgcaat tctttggcgc
    11281 tcgtgctaac ttggctaaaa cactattata tgccatcaat ggcggagtcg atgaagtgac
    11341 tggcgcccaa gttggaccag aatatcgtcc aatcacatca gaaatactag actttgaaga
    11401 agtgaaacat aacttcatga atatgatgga ttggttagca gaactatatg tcaatacgtt
    11461 gaacatcatt cattatatgc atgataaata ttcttatgaa gcagctcagc tggctttgat
    11521 ggattctgac ttgcaacgca catttgctac aggtattgct gggatctctc atgcggcaga
    11581 cagcttgtca gcgatcaaat atgcaaaagt aaaagcaatc cgtaatgaaa aaggaatagc
    11641 agttgatttt gaagtggatg gcgacttccc taaatacgga aatgatgatg accgtgcaga
    11701 tgaattagtt tcatggatct tggaatactt catgggacaa atcaaacgtc ataaaactta
    11761 ccgcaactca aaaccaacaa cttcattatt gacgattact tcaaatgttg tttacggtaa
    11821 aaatacagga aatactcctg atggccgtga agctggaaaa ccacttgcgc caggagcaaa
    11881 tccaagttac aatgcggaac aaagtgggtt attagctagc ctgaactcaa cagctaaaat
    11941 gaattacggc tgtgcattgg atggaatttc aaatacccaa acaatcaatc cttcaggtct
    12001 tggaaaagaa caagatacaa gaatcgaaaa cttgcgtaat gtcttagatg gatacttctc
    12061 aaaaggagct tatcatttga atgtcaatgt attcagtaat gatttattgc gtgatgcaat
    12121 ggaaaatcca atgaaatatc cgaacttgac gatccgggta tctggttatg cagttaaatt
    12181 ccgtgacttg acaagagaac aacaattaga tgtcttgatg agaacttcac acgacagaat
    12241 gtaaaaagtt cgtgaaaaaa gtttttcgac ttagcgtcga aaggacgttg aatagccatc
    12301 gcatcaaaat cgaataatca gtatgcttat ttgaaagaaa cgatttacag gggcaggact
    12361 tgtatgttcc gtccctgtaa attttacaca taacgaagcg gcaattgcga aaaattttgt
    12421 tcagaaagct tcgcttcgac agctaacttg tacttaaaat gaaatcaaag aaaagatgtg
    12481 aacttaaatg gaagaaaaaa caatcggcta tgttcattct atcgaaactt tcggttcagt
    12541 agacggacct ggactgcgct ttgtcgtgtt tatgcaagga tgccgcatgc gttgccaatt
    12601 ttgtcacaat ccagatactt ggaatattgg cggagggaaa gaatacacag cagatgaact
    12661 tttagataaa gcagaacgct tcagacctta ctggggagat aaaggtggta ttactgttag
    12721 tggtggagaa cctctattgc agatcgattt tttgatcgaa ctatttaaaa aagcaaaaga
    12781 gcggaagatg catactacat tggatacatg cggaaaacct tttacgtatg aagaaccttt
    12841 cttttcacgt tttcaagaat tgatgaagta tacagattta ctgttatttg acatcaaaca
    12901 catcgataat gaagagcata aaaaactaac gcattgggac aatgaaaata ttttagaaat
    12961 ggctcaatat ttgtcaaaaa tcaacaaacc ggtttggatt cgccatgtac tagttcctga
    13021 gcgaagtgat tatgatgaat atttgatccg actagacaac tttattcaaa ccttgtcaaa
    13081 tgtagacaga gtagagattc ttccttatca tacgatggga aaatacaagt gggaaacact
    13141 tggtttgaaa tatccattag aagggatcga accacctaca aaagaacgtg tcgaaaatgc
    13201 taaacgattg cttcatacag aagagtatac aaaatattta acgcgatagg tcgcatcgga
    13261 aagaagataa gtattgcttc tttccgtttt tttgctatta ttaaatagga gaaatcacaa
    13321 aggagcgaat gacatgtcaa aaattttagt ttttggtcac caaaatccag atacagatgc
    13381 aatcggggca gccattgcgt tttcacactt gcaaaatgaa ttaggtaaag aaacagaagc
    13441 agttgctcta ggtacaccta atgaagaaac acaatacgca ttagaccact ttcagctatc
    13501 tgcaccacga gtagtagagt cagctaaagc agaaaatgca gatgtcatgc ttgtggatca
    13561 taatgaattc caacaaagta tttcagatat cagcgaagta aatattttag ctgtagtaga
    13621 ccaccatcgt attgcaaact ttgaaacagc taatccattg tactatcgtg cagaaccagt
    13681 tggatgtaca agtacgatta ttttaaaact atataaagaa aaccaagtaa caattcctaa
    13741 agaaatcgca ggaatcatgc tctcagctat catttcagat acattgttgt tcaaatcacc
    13801 aacatgtacg cctgaagatg aagcagctgc aaaagaactt gcagcaatcg ctgaagtcga
    13861 tctaaacact tatggacttg aaatgttgaa agcaggaaca aatctaagtg ataaatcagc
    13921 tgcggttctt cttgatttgg atgcaaaaag tttcccaatg ggcagttcca acgttcgtat
    13981 cgctcaagtc aatacagtgg accttaatga agtgatggat cgacaagcag agctagaagc
    14041 agcaatggaa gatgaaatga aagcaaatgg ctacgatctg ttcgtattag ttgtcacaaa
    14101 catcttagac agtgattcag aattacttgt tgctggtaaa ggaaaagaaa aagtagaaga
    14161 agctttccaa acaactttga ccaacaaccg tgctttgctt aaaggtgtcg tttcacgtaa
    14221 aaaacaagtc gtacctcaat tgacggctgt atttagctaa tcaaatggta gtatgaagca
    14281 ttcaaaatga atggataagc gattgatata aaaagaaaca tagaacatac taaaggccga
    14341 agaaatggag gagaataaca gtcaattatg actcttgttc ttcctccttt tt
//
LOCUS       DACDXO010000066        14376 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E23605_2017
            SAMN12639989-rid9881463.denovo.067, whole genome shotgun sequence.
ACCESSION   DACDXO010000066 DACDXO010000000
VERSION     DACDXO010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639989
            Sequence Read Archive: SRR10023389
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741914.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:42:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,972
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 33 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 17 of 73
            Pseudo Genes (multiple problems)  :: 10 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14376
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639989-rid9881463.denovo.067"
                     /isolate="E23605_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            142..523
                     /locus_tag="IWM35_002229"
                     /pseudo
     CDS             142..523
                     /locus_tag="IWM35_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303242.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            654..1604
                     /locus_tag="IWM35_002230"
     CDS             654..1604
                     /locus_tag="IWM35_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002821555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAP9920068.1"
                     /translation="MLKTYSLTKDTLLVSSESNIKNSSVVYVYGDEREYIESSEHKYD
                     FKIGNILTFDDRVAYDTMIDKNDEKSLLVSFLFPEVHEGGHLDTLTTSVVFLVFKNKL
                     IIFTKQKLKFIPELLSNMVLRDVNHFVEEVLLKIMQKDFYMMLNDLRGVKEKIDDLDS
                     EISTSGSLKPTFGDLLTLQKYMIALSTTYKANHKALDFIKKNFTTIDDIDFSTKKIID
                     DLYDTSDTMDRIILSYSQYLDKLENMINNMISYQLNMIMKTLTEISIVLTIPAIIFGF
                     WGINVHVPFEKSSYGVLAVLIISAILSLICWLLMRRKTYL"
     gene            2122..2577
                     /locus_tag="IWM35_002231"
     CDS             2122..2577
                     /locus_tag="IWM35_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP9920069.1"
                     /translation="MVQQIVLPIKDSSVLKMVQDTLLDSFRAGRRNYTVFQVGKATLL
                     RVSDVMTLKKSDVYNPDGSVKNATFIHDKKTGKANTLYLKPVQQDLLQYHDWLAQENI
                     NSEWLFPSTAHYDLHITEKQFHKVMAHVGDLLGINLEDKEKKLRVRKPF"
     gene            2888..3244
                     /locus_tag="IWM35_002232"
                     /pseudo
     CDS             2888..3244
                     /locus_tag="IWM35_002232"
                     /inference="COORDINATES: protein motif:HMM:NF013878.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3257..3667
                     /locus_tag="IWM35_002233"
                     /pseudo
     CDS             3257..3667
                     /locus_tag="IWM35_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812298.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
     gene            3698..3946
                     /locus_tag="IWM35_002234"
     CDS             3698..3946
                     /locus_tag="IWM35_002234"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase I"
                     /protein_id="HAP9920070.1"
                     /translation="MQSKVNQLYKATASQTLKAMQGLYEAKLLSYPRTDTPFITENEF
                     AYLKANFGKYSGFLGLDLEMLLSLIMIRSNNSTQILTN"
     gene            complement(4097..5076)
                     /locus_tag="IWM35_002235"
                     /pseudo
     CDS             complement(4097..5076)
                     /locus_tag="IWM35_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332307.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(5081..5848)
                     /locus_tag="IWM35_002236"
     CDS             complement(5081..5848)
                     /locus_tag="IWM35_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP9920071.1"
                     /translation="MNKKRRLEKILDMLKIDGTITIKEIIDELDISDMTARRDLDALE
                     ADGLLTRTHGGAQLLSSKKPLEKTHIEKKSLNTKEKIDIAKKACSLIKDGDTIFIGPG
                     TTLEQLALELKGRKGYKIRVITNSLPVFLILNDSETIDLLLLGGEYREITGAFVGSMA
                     STNLKAIRFAKAFVSANAVTHNSIATYSDKEGVIQQLALNNAVEKFLLVDSTKFDRYD
                     FFNFYNLDQLDTIITDNQISPQHLEEFSQYTTILKAD"
     gene            6338..6763
                     /gene="lacA"
                     /locus_tag="IWM35_002237"
     CDS             6338..6763
                     /gene="lacA"
                     /locus_tag="IWM35_002237"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
                     /protein_id="HAP9920072.1"
                     /translation="MAIVVGADLKGTRLKDVVKNFLVEEGFEVIDVTKDGQDFVDVTL
                     AVASEVNKDEQNLGIVIDAYGAGPFMVATKIKGMVAAEVSDERSAYMTRGHNNSRMIT
                     VGAEIVGDELAKNIAKGFVNGKYDGGRHQIRVDMLNKMC"
     gene            6780..7295
                     /gene="lacB"
                     /locus_tag="IWM35_002238"
     CDS             6780..7295
                     /gene="lacB"
                     /locus_tag="IWM35_002238"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacB"
                     /protein_id="HAP9920073.1"
                     /translation="MRIAIGCDHIVTDEKMAVSEFLKSKGHEVLDFGTYDHARTHYPI
                     YGKKVGEAVVSGQADLGVCICGTGVGINNAVNKVPGVRSALVRDMTSAIYAKEELNAN
                     VIGFGGKITGGLLMNDIIEAFIEAEYKPTEENKKLIAKIEHVETHNAHQADEDFFTEF
                     LERWDRGDYHD"
     gene            7306..8238
                     /gene="lacC"
                     /locus_tag="IWM35_002239"
     CDS             7306..8238
                     /gene="lacC"
                     /locus_tag="IWM35_002239"
                     /EC_number="2.7.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
                     /protein_id="HAP9920074.1"
                     /translation="MILTVTLNPSVDISYPLETLKIDTVNRVKDVSKTAGGKGLNVTR
                     VLYESGDKVTATGFLGGKIGEFIESELEQSPVSPAFYKISGNTRNCIAILHEGNQTEI
                     LEQGPTISHEEAEGFLDHYSNLIKQSEVVTISGSLPSGLPNDYYEKLIQLASDEGVAV
                     VLDCSGAPLETVLKSSAKPTAIKPNNEELSQLLGKEVTKDIEELKDVLKESLFSGIEW
                     IVVSLGRNGAFAKHGDVFYKVDIPDIPVVNPVGSGDSTVAGIASALNSKKSDADLLKH
                     AMTLGMLNAQETMTGHVNMTNYETLNSQIGVKEV"
     gene            8241..9221
                     /gene="lacD"
                     /locus_tag="IWM35_002240"
     CDS             8241..9221
                     /gene="lacD"
                     /locus_tag="IWM35_002240"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HAP9920075.1"
                     /translation="MVLTEQKRKSLEKISDKNGVISALAFDQRGALKRLMAQYQDTEP
                     TVAQMEELKVLVADELTKYASSMLLDPEYGLPATKALDKEAGLLLAYEKTGYDTSSTK
                     RLPDCLDVWSAKRIKEQGADAVKFLLYYDVDSSDELNQQKQAYIERVGSECVAEDIPF
                     FLEILAYDEEISDAGSVEYAKVKPRKVIEAMKVFSDPRFNIDVLKVEVPVNVKYVEGF
                     ADGEVVYSKAEAADFFKAQEEATNLPYIYLSAGVSAKLFQETLQFAHDSGAKFNGVLC
                     GRATWAGSVEPYIKEGEKAAREWLRTTGFENIDELNKVLVKTASPWTDKV"
     gene            9249..9566
                     /locus_tag="IWM35_002241"
     CDS             9249..9566
                     /locus_tag="IWM35_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011669100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAP9920076.1"
                     /translation="MNREEMTLLGFEIVAYAGDARSKLLEALKAAENGDFAKADSLVV
                     EAGSCIAEAHNSQTAMLAREASGEELPYSVTMMHGQDHLMTTVLLKDVIHHLIELYKR
                     GAK"
     gene            9566..11272
                     /locus_tag="IWM35_002242"
     CDS             9566..11272
                     /locus_tag="IWM35_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9920077.1"
                     /translation="MHKLIELIEKGKPFFEKISRNIYLRAIRDGFIAGMPVILFSSIF
                     ILIAYVPNAWGFHWSKDIETFLMTPYSYSMGILAFFVGGTTAKALTDSMNRDLPATNQ
                     INFLSTMLASMVGFLLMAAEPAKEGGFLTAFTGTKGLLTAFIAAFVTVNVYKVCVKNN
                     VTIRMPEEVPPNISQVFKDLIPFTVAVVLLYGFELIVKGTLGVTVAESIGTLLAPLFS
                     AADGYLGITLIFGAYAFFWFVGIHGPSIVEPAIAAITYANIDANLHLIQAGQHADKVI
                     TSGTQMFIVTMGGTGATLIVPFLFMWICKSERNRAIGRASVVPTFFGVNEPILFGAPI
                     VLNPIFFVPFIFAPIVNVWIFKFFVDTLNMNSFSANLPWVTPGPLGIVLGTNFQVLSF
                     ILAGLLVVVDTIIYYPFVKVYDEQILEEERSGKTNDALKEKVAANFNTAKADAVLGKA
                     GVAKEDVAANNNITKETNVLVLCAGGGTSGLLANALNKAAAEYNVPVKAAAGSYGAHR
                     EMLPEFDLVILAPQVASNFDDMKAETDKLGIKLAKTEGAQYIKLTRDGQGALAFVQQQ
                     FD"
     gene            11414..12820
                     /gene="lacG"
                     /locus_tag="IWM35_002243"
     CDS             11414..12820
                     /gene="lacG"
                     /locus_tag="IWM35_002243"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HAP9920078.1"
                     /translation="MTKTLPKDFIFGGATAAYQAEGATHTDGKGPVAWDKYLEDNYWY
                     TAEPASDFYHKYPVDLELAEKYGVNGIRISIAWSRIFPTGYGEVNEKGVEFYHNLFAE
                     CHKRHVEPFVTLHHFDTPEVLHSDGDFLNRENIEHFVDYAAFCFEEFPEVNYWTTFNE
                     IGPIGDGQYLVGKFPPGIQYDLAKVFQSHHNMMVSHARAVKLYKDKGYKGEIGVVHAL
                     PTKYPYDPENPADVRAAELEDIIHNKFILDATYLGHYSDKTMEGVNHILAENGGELDL
                     RDEDFQALEAAKDLNDFLGINYYMSDWMQAFDGETEIIHNGKGEKGSSKYQIKGVGRR
                     IAPDYVPRTDWDWIIYPEGLYDQIMRVKNDYPNYKKIYITENGLGYKDEFVDNTVYDD
                     GRIDYVKKHLEVLSDAIADGANVKGYFIWSLMDVFSWSNGYEKRYGLFYVDFDTQERY
                     PKKSAHWYKKVAETQVIE"
     gene            12843..13115
                     /locus_tag="IWM35_002244"
     CDS             12843..13115
                     /locus_tag="IWM35_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3884 family protein"
                     /protein_id="HAP9920079.1"
                     /translation="MLSDTLEKVQHLFRPGFEKVYIVSFEDCPMIPELEATPLLKCGK
                     WYVSTGKEWICHSDLELSAFEWEFLQSLDVEIRETIHFEVNYLPFQ"
     gene            13136..14035
                     /locus_tag="IWM35_002245"
     CDS             13136..14035
                     /locus_tag="IWM35_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002889672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HAP9920080.1"
                     /translation="MTIELKNEYLTVQFKTLGGQLTSIKDKDGIEYLWQADPNYWNGQ
                     APILFPICGSLRNDWAIYRPQERPFFTGLIRRHGFVRKEEFILEEVNDNSVTFSIKPN
                     AEMLDNYLYQFELRVVYTLNGKSIRTEFQVTNLEMEKTMPYFIGAHPAFNSPLVEGEN
                     YEDYYIEFSEVESCSIPKSFPETGLLDLQDRTPFLENQKILDLDYSLFSHDAITLDQL
                     KSRSVTLRSRKSGKGLRVDFDDFPNLILWSTTNKSPFIALEPWSGLSTSLEEGNFLED
                     KRQVTKISPQETSRKIYDITILN"
BASE COUNT         4695 a         2371 c         2771 g         4539 t
ORIGIN      
        1 gcgtttgatt ttcaaacttc gcaacagaac cgatgacact atcgttcttc atcaacgtaa
       61 gttagatttg ttgaaagagc agaaaaaagg atttctacaa aaaatgtttg tttgatattt
      121 taaatagaaa agagagataa agtgaagttt ttaaattttt tgaactataa atatttaaac
      181 tgggtattat atggtattac attatttttt attatttatt acttttttac cgatgatgat
      241 atttttttaa ttttagcaat actttttctt gtttcatcta attttagtga taacttaatt
      301 gaagttctta cagaaccaga aattcggtct atgacggatg aagaacaaca gattattaaa
      361 gctatgattt cggaaaataa gaaagacgta gtagttatca aaaagattag agagtataca
      421 aatttaggtg ttttacaagc caaaaaagta tatgaaaaaa aaaaaaaggg tctataatta
      481 caaattagat aatacattag aagctggaga caagattgtc tagataatgt gtgagataaa
      541 atttttggca ggttatcata cataagacaa aatgttttta aaactgataa cgtatttatt
      601 ccacaacaat tacgcattta tttctgagaa aaatataaag caggaggatt acaatgctga
      661 aaacttattc cttaacaaaa gatacattat tagtttcgtc agaatcaaat atcaaaaatt
      721 cttctgttgt ttatgtttat ggtgacgaac gagaatatat cgaatcttct gaacataaat
      781 atgactttaa aataggcaat atactaacgt ttgatgatcg agttgcatat gacacgatga
      841 tagataagaa cgatgaaaaa agtttgctcg taagtttttt gtttcccgag gtacatgaag
      901 gtgggcatct agataccctg acgacatctg ttgttttctt ggtatttaaa aataagttga
      961 taatttttac aaaacaaaaa ttaaaattca ttcccgaact cttatccaat atggttttac
     1021 gtgatgtcaa tcatttcgtg gaggaagtac ttttaaagat catgcaaaaa gatttctata
     1081 tgatgcttaa cgaccttcgt ggagtcaagg aaaaaattga tgatttagac tcagaaatat
     1141 caacgtccgg ttcactcaaa ccaacgtttg gtgatttatt gactcttcaa aaatacatga
     1201 tagctttgtc aacaacttat aaagctaacc ataaagcgct tgatttcatt aaaaaaaatt
     1261 tcacaactat tgacgatatt gattttagta ctaaaaaaat aattgatgat ttatacgata
     1321 caagtgatac aatggataga atcattttaa gttacagcca atatttggac aaattggaaa
     1381 atatgataaa caatatgatt tcgtatcagc taaacatgat tatgaaaacc ctaaccgaga
     1441 tatcaattgt tttgactatt ccagcaatta tttttggctt ttggggaatc aatgtccatg
     1501 tcccgtttga aaagtcttct tatggtgttt tagccgtatt gattatctct gctatactaa
     1561 gtctaatttg ctggcttttg atgcgacgca aaacatattt gtaaagtatt tgtgataccc
     1621 taatatgatt taaagcagaa tttctttgtt gactttttaa aaggctgatt cctttcaata
     1681 atttaatagc ttcagcggct gctcagaagt caataaattg gactattttt attcagttac
     1741 ttaggcaaca tttgagatac gattgcgatt ataaaaattg atataccaac caatggctgc
     1801 ttggaagact gacatattag cctcgatatc atcgaataac taaatacaga aaaatacaat
     1861 aaatattatc aaactaggga ctatactaga aaacatcagt acattcctta actcagggtc
     1921 tataattaga aaatcgcccc tttgaaaact gattaaaaca gcccccatta tctagtgact
     1981 agataatggg ggctgttttt ttgttaagtg atataataaa ccataaaaga agtttagaaa
     2041 tacggtcctg gtcatcaata aataaacaat ttaattaatt atttatttaa ttatttaatt
     2101 tcagaaaggt atacatgaat catggtgcaa caaattgtcc tacccatcaa agactccagc
     2161 gtcttaaaga tggtacaaga tacacttctc gatagtttcc gtgctggtcg ccgaaattac
     2221 accgtttttc aagttggtaa ggccacgcta ctacgtgtga gtgatgtcat gacattaaaa
     2281 aaatcagatg tctataatcc agacggctct gtcaaaaatg caacctttat tcatgataaa
     2341 aagaccggta aagcaaacac gttatattta aagcctgttc agcaagactt gttgcaatat
     2401 catgattggc tggcccaaga aaatatcaat tctgagtggt tatttccctc gacagcccat
     2461 tatgatcttc atatcactga gaagcagttt cataaggtga tggcgcatgt tggtgatctc
     2521 ttaggaatca acttggaaga caaagagaaa aaattgcgag tcagaaaacc gttttgagat
     2581 atgaacagca tttgccttca cgccttagcg attttaaata atagccttga aagtgattat
     2641 ggaaaagccc tctacgctca atgcaaaatt gacgtagaaa ctatttttga tcgattgaaa
     2701 ggtgtatttg gtatgtgaag aacccatgtc agaggcaaga acgttgcacc tactgacatt
     2761 ggaatgatgc ttatgagcat gaatctaacc aaattatccc ttgaggctag aagaaaagcg
     2821 gaagcttttc aacacaaagc agccaaaaat aaaaaacgcg acgaagcaat cacatttatg
     2881 attattcatc gcgtttttta tatttagagc tagttatttg ctagacccac tatttacaga
     2941 tgaaacgttt atcacctttg gttttgggca tttagtggaa ttagcagaac caggtcatta
     3001 tgacgaaaag tggcaaaatt ggaaacttga atctttgccg atttttcctg atcgatacga
     3061 ttttgaagtt gcaaaagata agggaaagca gtttaaaatt gttgcagaac ttctcaaaaa
     3121 ggcaaataca attattgttg caacagatag cgacagagaa ggtgaaaata tcgcctggtc
     3181 gattatccat aatgcaaatg ccttttcaaa agataaaaca tttaaaagac tatggatcaa
     3241 tagcttagaa aaagatgtga tccgaagcgg ttttcaaaat ttgcaaccag gaatgaatta
     3301 ctatcccttt tatcaagaag cgcaaacacg ccaaattgcc gattggttga tcggcatgaa
     3361 cgcaagccct ttgtatacgt taaatttaca acagaagggc gtacaaggta cattttcact
     3421 aggacgtgtt caaacgccca ccttatacct tatttttcag cgccaggaag ccatagagaa
     3481 ttttaaaaaa gaaccttttt tcgaggtgga agctaagtaa aacaagggtc atttaagggc
     3541 gttctaagcc ccacacagcg ttttaaaacc caagaggagc tttttagctt ttgtttcttc
     3601 taaacaagct aaaataggca atcaagaggg gataattgct gatgttcaaa ccaaagagaa
     3661 gaaaacgaat agtccgagtt cgttttcttt aagtagtttg caatcaaaag tcaatcagct
     3721 ttataaagcg acagcgagcc aaactttaaa agctatgcaa ggactgtatg aagcaaaatt
     3781 attgagttat ccaagaacag atacaccatt tattacagag aacgaatttg cttatttaaa
     3841 agcgaatttt ggcaaatata gcggtttttt aggacttgat cttgaaatgt tgttatcatt
     3901 gataatgata cgttcaaaca acagcaccca aattttgacg aattagtgaa actctatgaa
     3961 aaagacgtag taaaacatgc aacatcctat tctaatcagc tggtaaagct taattaggtt
     4021 atataagctt gaaaataaag ggtttgtcca atatggacaa gcctttttct tatgttttgc
     4081 cacagatttg ccacacttat tgtgtaagag tatcaaggat aggtataatt tgatactttg
     4141 tttttttagt gatgtgcaaa taaatttctg ttacacgact acctctagaa tgccctacac
     4201 ggtcttgaat tgcttctagg ggtattcctt actctgctaa gaaactaata tgcgtatgac
     4261 ggaatatatg ggtcgtaata tgtttattaa aactaagttt attatcaata tcaattcctg
     4321 ctcgttttgg attacctttt cgatttaatt ttccattttc aggtgagttg aggaaattgt
     4381 taaggacact ataatgaaga ggtgttccat tatagtcggg tttatgacga cttttggaag
     4441 taaaaatcca tccattatct ttataatcag gattgaatct catatgttgg tgatttaact
     4501 caatttcctc tttaagaatt tctatagccc ttttagataa ttggacattt cggtaacttt
     4561 gtaagttttt tacattacct tcatagctat tttctgcagc tcctccgtga gtggactgac
     4621 taatgatgat attttgcttt tcaaaatatt gataggcacc aatattttgg agtttcgact
     4681 ctgtaagtca acagggccat tagttagaat acctattggg atgcggtttt tatgtagagc
     4741 cagcagtagt tgatgtatct caggaatcat cgtaatctga tcgagttcat attcatagtc
     4801 agcttggaag tctagagctt ctgtcctaga aatactatcg atgccaaatt cttccatagt
     4861 cttctgacag cgaaaaattc taagctgctc aatactgatg agttttttcg tgtatttagg
     4921 aaaagcaaca tcagaccaat atctgaaacg tttgtaaatg ttatctattt gttggatatc
     4981 aatcgttgga aaacatttct caacgctacg tttaaagggg agttgatggt cataaagagt
     5041 gtcatcaagg tcaaaaacaa tatttttata agtcataatt ctagtccgct tttaaaatag
     5101 tagtgtactg gctaaattcc tctaagtgtt gagggctaat ctggttatct gtaatgatgg
     5161 tatcgagttg atctagatta tagaagttaa agaaatcgta tcgatcgaat ttagtactgt
     5221 ctactaataa gaatttttct acagcattgt ttagggcaag ttgttgaatc acaccttcct
     5281 tgtcactata tgtagcaata gaattatggg taacagcatt tgcactaaca aaagctttgg
     5341 caaatctaat tgcttttaaa tttgtcgaag ccattgaacc tacaaaagct ccagttattt
     5401 ctctatattc accgccaaga agcaataaat caatggtttc gctatcattt agaatcaaga
     5461 acacagggag actatttgta atgacacgaa ttttataacc tttacgacct ttcaattcta
     5521 atgccagttg ttcaagtgta gttccaggtc caataaaaat agtatcgcca tctttgatta
     5581 aagagcaggc ttttttagca atgtcaattt tttcttttgt atttagactt ttcttctcga
     5641 tatgtgtctt ttcaagtggc tttttagagg aaagcaattg tgcaccacca tgagtacgtg
     5701 ttaaaagtcc atcagcttct agagcatcaa ggtctctacg ggctgtcata tcggaaatat
     5761 ctagttcatc tattatttct tttatggtta tggtcccatc aatctttaac atatctaaaa
     5821 ttttttctaa tcgtcgtttt ttgttcatat gaagaccctt ctttcataaa gtaatttttt
     5881 tccaaagata attctctttt aattgtatca taaaagacaa tattttcaag gtaaaacaaa
     5941 caatttcaaa caaaaacaaa tgttagatga tgaaataaga acagaggatt gacgtatatt
     6001 agcttaggtc agattttgta taagacgaaa ataaagtagg acctcttaat cagtaagtta
     6061 tagaaagtaa aagacttttg taatacttga atagatattt cacgtccatt ttgtgatgga
     6121 ttaaatgaac aaaaatgaac aataatttaa cggtgttatc tattttttaa aaaaacaaat
     6181 aaaaaaaaca aaaaataaac aaaaaaagtg ttgctttttg tttgaatgct tgatattata
     6241 taaacaaaga aatgatgaaa acgttttttc gaacattttg caaaatgttt tctacttcta
     6301 cgtagcattt ctttctaaaa tttaggaggt ggtcaaaatg gctattgttg ttggtgcaga
     6361 tctcaaaggt acgagattaa aagatgttgt gaaaaatttt cttgtagaag aaggttttga
     6421 ggtaattgat gtgactaagg acggacaaga ttttgttgat gttaccttgg cagttgcctc
     6481 tgaagtaaat aaagatgagc aaaacttagg tattgtaatt gatgcttatg gagctggtcc
     6541 attcatggta gcaactaaaa ttaaaggtat ggttgcagca gaagtttcag atgaacgctc
     6601 agcttatatg acacgtggac ataacaattc acgaatgatt acggttggcg ctgaaatcgt
     6661 cggtgatgag cttgctaaaa acatcgctaa aggtttcgtc aatggtaaat atgatggcgg
     6721 acgtcatcaa atccgagtag atatgcttaa caaaatgtgt taagaaaagg agttaaggaa
     6781 tgagaatcgc aattggatgt gaccacattg tcacagatga aaaaatggca gtatcagaat
     6841 tcttgaaatc aaaaggacat gaggtccttg atttcgggac atatgaccac gcacgtactc
     6901 actaccctat ctatggtaaa aaagtcggtg aagcagtagt aagtggtcaa gcagacttgg
     6961 gagtatgtat ctgtggtaca ggtgtcggca ttaacaatgc agtcaataaa gttccaggtg
     7021 ttcgctcagc gttggttcgt gatatgacat cagctatata tgcaaaagaa gagttaaatg
     7081 cgaatgttat cggtttcggt ggaaagatta ctggtggtct tcttatgaat gacatcattg
     7141 aagctttcat tgaagctgag tacaaaccaa cagaagaaaa taaaaaattg attgcaaaaa
     7201 tcgaacacgt tgaaacacat aatgcacatc aagcagatga agatttcttt acagaattcc
     7261 ttgaaagatg ggaccgtggt gactaccacg attaaggtat aacaaatgat tctgacagtc
     7321 acactaaatc cttcagtaga catctcttat cctctagaaa cattgaaaat tgatacagtg
     7381 aatagggtga aagatgttag taagacagct ggtggaaaag gcttaaatgt tactagagtc
     7441 ttgtatgaat ctggtgataa agttactgct acaggtttct taggtggtaa aattggtgaa
     7501 tttattgaaa gcgaattaga acaatctcct gttagtccag ctttttataa aatctcagga
     7561 aacactagaa actgtattgc tatcttacac gagggtaacc aaacagaaat tttggaacaa
     7621 ggaccaacaa tttctcatga agaagcagaa ggctttcttg accactatag caatcttatt
     7681 aaacaatcag aagttgtgac aatttcagga agtttacctt caggacttcc caacgattat
     7741 tatgaaaaac ttatccaact tgcttcggat gaaggagtag cagtcgtttt ggattgttca
     7801 ggtgcacctc ttgaaacagt tctaaaatcg tcggctaaac ctactgccat caaacctaat
     7861 aatgaggaac tttcacagtt gcttggaaaa gaagtaacaa aagatattga agaactcaaa
     7921 gacgttctta aagagtcact ttttagtggt atcgaatgga tagtggtctc attaggtcga
     7981 aacggtgcct ttgcaaaaca tggtgatgtt ttctataagg tagatattcc tgatatccca
     8041 gttgttaatc cagtcggatc aggggactca acggtagctg gtattgcatc agctttaaat
     8101 agtaaaaaaa gtgacgctga cttattaaaa catgcgatga cattgggtat gttaaatgct
     8161 caagaaacaa tgacagggca tgtgaatatg actaattacg aaacactaaa ctcacaaatt
     8221 ggagtaaaag aggtataaaa atggtactta cagaacagaa acgcaaatct ttggaaaaaa
     8281 tttcagataa aaacggtgtt atctcagctt tggcatttga ccaacgtggt gctttgaaac
     8341 gtttgatggc acagtatcaa gatacagaac caactgtagc tcaaatggaa gaactaaagg
     8401 ttttggttgc tgatgaactt acaaaatacg cttcttcaat gttgcttgac cctgaatatg
     8461 gacttccagc aacaaaagca ttggataaag aagcaggtct tcttcttgcc tatgaaaaaa
     8521 caggttatga tacatcaagt acaaaacgtt tgcctgactg cttggatgtt tggtctgcaa
     8581 aacgtatcaa agagcaaggc gctgatgccg ttaagttctt gctttactat gatgtagaca
     8641 gctcagatga acttaaccaa caaaaacaag cttatattga acgtgttggt tctgaatgtg
     8701 tcgctgaaga tattcctttc ttcttggaaa tccttgctta tgatgaagaa atctcagatg
     8761 caggttctgt tgaatatgca aaagtgaaac ctcgcaaagt cattgaagcg atgaaagttt
     8821 tctctgatcc acgctttaac attgatgttc ttaaagtaga agtaccagtt aacgttaaat
     8881 atgtcgaagg ttttgctgat ggtgaagtag tttacagtaa agctgaagct gctgacttct
     8941 ttaaagcaca agaagaagcg actaaccttc catacatcta cctaagtgca ggtgtatctg
     9001 ctaaattgtt ccaagaaaca ttgcaatttg ctcacgactc aggtgccaag tttaatggtg
     9061 tgctttgtgg acgtgctact tgggcaggat ctgtagaacc ttacatcaaa gaaggtgaaa
     9121 aagcagcacg tgaatggttg cgtactactg gattcgaaaa tattgatgaa cttaacaaag
     9181 ttcttgttaa aacagctagt ccatggactg ataaagtata gtaaaaacat aaaacggagg
     9241 atattgttat gaacagagaa gagatgactc tcttagggtt tgaaattgtt gcttatgctg
     9301 gagatgctcg ctctaaactt ttagaagcgc ttaaagcggc tgaaaatggt gatttcgcta
     9361 aggcagatag tcttgtagta gaagcaggaa gctgtattgc agaggctcac aattctcaga
     9421 cagctatgtt ggctcgagaa gcttctgggg aggaacttcc atacagcgtt accatgatgc
     9481 atggtcagga ccacttaatg actactgtct tattaaaaga tgtgattcat cacctcatcg
     9541 aactttataa aagaggagca aaataatgca taaactcatt gaacttattg agaaagggaa
     9601 accgttcttt gagaaaatct ctcgaaacat ctatcttcgt gctattcgtg atggatttat
     9661 cgctggtatg ccagtcatcc ttttctcatc tatctttatc cttattgcct atgtcccaaa
     9721 tgcttgggga ttccactggt caaaagacat tgagaccttc ttgatgactc cttatagcta
     9781 ttcgatgggt atcctagcat tctttgttgg tggtactacc gctaaagctt tgacggactc
     9841 aatgaaccgt gacctacctg cgaccaatca gattaacttc ttatctacga tgctagcgtc
     9901 tatggtaggg ttccttttga tggcagctga gcctgcaaaa gaaggaggct tcttgacagc
     9961 cttcacggga acaaaaggtc ttttgacagc atttattgca gcctttgtga cagttaatgt
    10021 ttacaaggtc tgtgtgaaaa ataatgttac tattcgtatg cctgaagagg ttccaccaaa
    10081 tatctctcaa gtatttaaag acttgattcc attcactgta gcggttgttc tcctttatgg
    10141 atttgaactc attgttaagg gaactcttgg tgtaaccgtt gcagaatcaa tcggtaccct
    10201 tcttgctcct ctcttctcag ctgcagatgg ttaccttgga attacactta tctttggtgc
    10261 ttatgccttc ttctggtttg ttggtatcca cggtccttca attgtcgaac cagcaatcgc
    10321 tgctatcact tatgccaata tcgatgccaa cttgcatctt atccaagctg gacaacatgc
    10381 agacaaagtt atcacttctg gtactcaaat gtttattgtt accatgggtg gaacaggagc
    10441 tacattgatt gttccattct tgttcatgtg gatttgtaaa tcagaacgta accgtgccat
    10501 cggacgtgcc tcagttgttc caacattctt tggggttaat gagccaatct tatttggtgc
    10561 accaatcgta ttgaatccga ttttctttgt accgttcatt ttcgctccga ttgtcaacgt
    10621 ttggatcttt aaattctttg ttgacacatt gaacatgaac tcattctctg ccaaccttcc
    10681 atgggttact cctggtccgt tgggaattgt actcggtact aacttccaag ttctatcatt
    10741 tatcttagcc ggactcttgg tagttgttga tactatcatt tattacccat ttgttaaggt
    10801 atacgatgaa caaattcttg aagaagaacg ttctggtaaa acaaacgatg ctcttaaaga
    10861 aaaagtagct gcaaacttca atactgctaa agcagatgcc gttcttggaa aagcaggcgt
    10921 tgctaaagaa gatgtagcag caaacaataa tatcacaaaa gaaactaatg tccttgttct
    10981 ttgtgcaggt ggaggtacaa gtggcttgct tgccaatgcc ttgaacaaag cagcagcaga
    11041 atacaatgtt ccagtcaaag cggcagcggg tagttacggt gctcaccgtg aaatgttgcc
    11101 agagtttgac ttggttatct tggctccaca ggtcgcttca aacttcgatg atatgaaggc
    11161 tgaaacggat aaattgggca ttaaacttgc aaaaactgaa ggagctcaat atatcaaatt
    11221 gacacgtgat ggacaaggtg ctcttgcatt tgttcaacaa cagtttgatt aataaatttg
    11281 taaaagttat ttgagacgag attattcttt caattcctta taatattgtt aaccatatca
    11341 ttgcatttca tttccttggt ttccggaact cagaatatcg tctcaaatgc tttttttgaa
    11401 aggatttata gttatgacta aaacacttcc taaagatttt atttttggtg gtgctacagc
    11461 tgcttatcaa gcagaaggtg cgactcacac ggatggtaaa ggcccagttg catgggataa
    11521 gtatcttgaa gacaattatt ggtacacagc agagcctgca agtgattttt accataaata
    11581 tcctgttgat ttagaattag ctgaaaaata tggcgttaat ggtattcgta tttcaattgc
    11641 ctggtctcgt attttcccaa ctggctatgg cgaagtaaat gaaaaaggtg ttgaattcta
    11701 ccataatctt tttgccgagt gccacaaacg tcatgttgag ccattcgtga ctttgcacca
    11761 ctttgacaca ccagaagttc ttcactcaga tggagatttc ttaaaccgcg aaaatataga
    11821 acactttgta gattatgccg ctttctgttt tgaagagttc ccagaagtaa actactggac
    11881 aactttcaat gaaattggcc caattggaga tggtcaatac ttggttggta aattccctcc
    11941 aggcattcaa tatgatcttg ccaaagtctt ccaatcacac cacaatatga tggtttctca
    12001 tgcacgtgct gtgaaattgt ataaagataa aggttataaa ggcgaaattg gtgtagtaca
    12061 tgcgttgcca accaaatacc cttatgatcc agaaaatcca gcagatgttc gtgccgctga
    12121 gttagaagat atcatccata acaaatttat cttggatgca acctaccttg gacactattc
    12181 agataaaacg atggaagggg tcaaccacat cctagctgaa aatggtggag aacttgatct
    12241 tcgtgatgaa gacttccaag cacttgaagc agctaaagat ttgaacgatt tccttggtat
    12301 caactactac atgagtgatt ggatgcaagc ctttgatggt gagactgaaa tcattcacaa
    12361 tggtaagggt gaaaaaggaa gttctaagta ccagattaaa ggtgttggtc gccgaatcgc
    12421 accagattat gtgcctagaa cggattggga ctggattatt tatcctgaag gcttgtatga
    12481 ccaaatcatg cgagtgaaaa atgattatcc aaactacaag aagatttaca tcactgaaaa
    12541 cggtcttgga tacaaagatg agtttgtaga taataccgtt tatgatgatg gtcgtattga
    12601 ttacgtgaag aaacacttgg aagttttatc agacgcaatt gcggatggtg caaatgttaa
    12661 aggttacttc atctggtcac ttatggatgt cttctcatgg tcaaacggat acgaaaaacg
    12721 ttatggacta ttctatgtag actttgacac gcaagaacgc tatcctaaga aatcagctca
    12781 ttggtataag aaagtagcag aaactcaagt gatagagtaa tattacttga aaatgtaaaa
    12841 aattgttatc cgatacttta gaaaaagtac aacacttatt tagaccaggc tttgaaaagg
    12901 tatatattgt tagtttcgaa gattgtccta tgattcctga gcttgaagct accccgttgc
    12961 taaaatgtgg taaatggtat gtttcaactg gaaaagagtg gatttgccat tctgatttag
    13021 agttatcagc atttgaatgg gaatttttac aatcactcga tgtagaaata cgtgagacta
    13081 ttcacttcga ggtaaattat ttgcctttcc aataataaaa agaaaggaat tagttgtgac
    13141 tatcgaatta aaaaacgagt atcttactgt tcagtttaaa acacttggtg gtcaactgac
    13201 ttcgattaaa gacaaggatg ggatagagta tctctggcaa gctgatccaa attattggaa
    13261 tggacaggcg cctattctat ttcctatctg tggtagttta cgaaatgatt gggctattta
    13321 tagacctcaa gaaagaccct tttttacagg tcttattcgt agacatggtt ttgttcgaaa
    13381 agaagaattt attctggaag aagtcaatga taatagcgtg acctttagta ttaaaccaaa
    13441 tgctgagatg cttgataatt acctatatca gtttgaactg agagttgttt ataccttgaa
    13501 tggtaaatca attagaaccg aatttcaggt aacgaacttg gaaatggaaa aaacaatgcc
    13561 ttactttatt ggagcccatc cagcattcaa tagtccttta gtagaaggtg aaaactatga
    13621 ggattattat attgaattta gtgaggttga gtcttgctcg ataccaaaga gttttcctga
    13681 gacaggatta ttagatttgc aagatcggac accttttttg gaaaatcaaa aaattctcga
    13741 tttggactac tcactttttt cccatgatgc cattacactt gaccagctca agtctcgtag
    13801 cgtaacgctt cggtcaagga agtctggtaa aggtcttcga gtagattttg atgattttcc
    13861 gaacttgatt ctgtggtcta caactaataa aagtccattc attgctttgg aaccttggag
    13921 tggtctctcc acttcacttg aagaaggtaa tttcttagaa gataaaagac aggtaacgaa
    13981 aatttcacct caagaaactt ctagaaaaat ctatgatatt actatcctta attaaaattt
    14041 gttattattt gagtcttcaa taagatttga atactcttac cttgtgatga tagattggtc
    14101 ttctccgtgc cagtcttaca tcgcaaggtt tttaagtttt ttgtgggtct atgtcaactg
    14161 tagtgggtga cgaaaagcta atatctaaag acagtctggt agtaaaaaat aagattattt
    14221 actacttctt gatttaaaat aatagtggag ccattaattt gtggcaaatt taatttaata
    14281 ttttgcctca aattagccac actttttata gaactaagag gaaaaatgaa attttcttcc
    14341 tattaatata gttgtttgaa atcttaaata tagcgt
//
LOCUS       DACDXO010000067        14188 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E23605_2017
            SAMN12639989-rid9881463.denovo.068, whole genome shotgun sequence.
ACCESSION   DACDXO010000067 DACDXO010000000
VERSION     DACDXO010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639989
            Sequence Read Archive: SRR10023389
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14188)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14188)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741914.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:42:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,972
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 33 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 17 of 73
            Pseudo Genes (multiple problems)  :: 10 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14188
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639989-rid9881463.denovo.068"
                     /isolate="E23605_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(143..1345)
                     /locus_tag="IWM35_002246"
     CDS             complement(143..1345)
                     /locus_tag="IWM35_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP9920081.1"
                     /translation="MKTKYPCVYQDKNGRFSYLVELGTDQVTGKRIQKKGTKDSLGKR
                     FSSAREAHKEVMRIKNEYMVVNGFANYDLTYKEFMEQSYVPHYKASVQKSTWSSRECG
                     LIQITEFFGDKKLREINVRDCENYRIWLLNDSGYSQAYSSLMYGMFRKSLDYAVTLQF
                     LNENISKRTKAIPKGKSIVPYWTKEEFEEVLAKIYKDDFYEHMCFLMIWLYYMTGVRV
                     SEGLALNWNDVDFKRKKLRVHHTLEMTNQKDFIRKPYTKTESGLRIISLDDDTIEVLK
                     KWKVIQKNHGVEQFILSYTDLPLYRSTVQRIIQRYAKLAKVPLIQGKGLRHSHVSYLI
                     NEFNADILVVSQRLGHSSPEITLKHYAHLWSRNDEGIAEQMTGNIKFTCATKSKVANF
                     NGNQAVKI"
     gene            complement(1348..1542)
                     /locus_tag="IWM35_002247"
     CDS             complement(1348..1542)
                     /locus_tag="IWM35_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAP9920082.1"
                     /translation="MKNTICRKDLEALGFKTHQATTIIRQAKTRMVNQGYPYYNNKRV
                     GVVPRQVIEEIIGVPLSQEE"
     gene            complement(1589..2437)
                     /locus_tag="IWM35_002248"
     CDS             complement(1589..2437)
                     /locus_tag="IWM35_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rep protein"
                     /protein_id="HAP9920083.1"
                     /translation="MQAYNQKIIETPTYIEIYDYEAIITPKRKTIENEVNLERKQKLA
                     WIYENDITLEKRKNYDELSAQGQYDSLKRKQKHYQSMRFEIARLVDTNFDNQTKFLTL
                     TFKENIQDIEYANNEFKKFIKRLNYDLYKTKKANLKYLATWEKQQRGAIHYHIILFDF
                     PFISFNRLTTIWGQGFVKINKIDVDSIENRGRYISKYFDKELELKEHKKKAFFKSRNL
                     KLPTVTKKMVKKPYDSTNQDILYTSEYIVRRPFFYNALALNELGSPEQVMDFQESTVH
                     YTKIKK"
     gene            complement(2642..2992)
                     /locus_tag="IWM35_002249"
     CDS             complement(2642..2992)
                     /locus_tag="IWM35_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9920084.1"
                     /translation="MPIKITDLSLPKETLEKEYQVVNVTRWQKEGEILGWSYECILPK
                     LRYEKISVKVKSEHPVITPEELTQQGLAIITFTNLVIVPWGRSNGQFVSYGLSATADT
                     ATLIPRKQGTTTQN"
     gene            3587..4033
                     /locus_tag="IWM35_002250"
     CDS             3587..4033
                     /locus_tag="IWM35_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HAP9920085.1"
                     /translation="MKDYVVQITKEVLEESKTLNKLSSKAKEEAVTILSSGIIDYIEM
                     SGEIKYGEKDRIAKMVKESIKTFDVKQDLTFNDSYLIGKLINILGTDEDTELFLSSLS
                     MIFTDKHFSTRFGGEELVEIFQSMRPALIKLYREKTPDEFYDWFEK"
     gene            4657..5649
                     /locus_tag="IWM35_002251"
     CDS             4657..5649
                     /locus_tag="IWM35_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP9920086.1"
                     /translation="MYWEEIELEKTLGPGGEIRAVQRDKKNISKWEKLKKQLRRDKWM
                     YLLLLPGIIMTFIFKYVPMYGAVIAFKDYNPMLGILESPWVGLDHFREFISSPNFTLL
                     LSNTLKISIYGLLLGFFPPIILALSLNQIINKKIKQKIQLILYAPNFISVVIIVGMLF
                     LFLSANGPINGLVEKVTGQPIMFMSNPAYFRTIYILSGIWQGIGWSSILYTATLAGVS
                     TDLYEAANIDGANILQKIWHIDLPALRPVMVISFILAAGGIMNVGYEKAYLMQTSMNI
                     PASEIISTYVYKVGLQSGDYAYSAAVGLFNMIINVVLLLIVNNITKRLNDGQGL"
     gene            5665..6549
                     /locus_tag="IWM35_002252"
     CDS             5665..6549
                     /locus_tag="IWM35_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018165023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP9920087.1"
                     /translation="MNSALSKIDRQILWLNRVIVTFLVVITIVPLIYVLVASFMDPAT
                     LINKGISFNPKDWTIQGYQRVFADDSILRGFVNSLFYSFSFSLLTVFITMITAYPLSR
                     PNLAGKKIIMTFFVITMFVGGGLIPTYLLVKNLGMLDSVWAIIVPGAINVWNIILART
                     YFSALPKELSEAATIDGANDLQILFKVMLPLAKPIMFVLFLYAFVGQWNSYFDAMIYI
                     KDPDLQPLQLVLRNILIQNQPSQDMVGANTAMAEMKQIAELIKYATIVISSLPLIIMY
                     PFFQKYFDKGVLVGSVKG"
     gene            6598..8205
                     /locus_tag="IWM35_002253"
     CDS             6598..8205
                     /locus_tag="IWM35_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP9920088.1"
                     /translation="MKAKWMLPIVFTSVLILGACGGSKEKSKSSPDYELENVSFPLKE
                     KVSLKMMSQSAPLAPNDPNDKLIFQRLEEETNLHIDWTNYSSDFAEKRNLDIASGDLP
                     DAIFNAAAGDYDLLNWAESGVIVPIEDLIEDYMPNLKKIFDENPEYRQMSTAPDGHIY
                     SMPWIEELGEGKESIHTVNGMAWINKEWLDNLGLEMPQTTDQLMTVLEAFKTQDPNGN
                     GEADEIPFSFINNGGNEDLKFLMGAFGLGDNDDHLVVDNDGQVQFTANKEGYKEAIKY
                     FNEMNNKGLIDKEAFEQDWNTYVAKGKEHKYGVYFTWDKANITGADDSYDVLPVLAGP
                     DGTKHVTRTNGMGFSRDRFVITSANKNLELTARWIDKMYEPLQSVQNNWGTYGDEAQA
                     NIFELKDGMLKHLPLEGTAPGELRQKTEAGGPLAVLDSYYGTVTTMPDDAKWRLDLMH
                     DTYLPYITEDNIYPRVFLEKEETDRLAKIETDMWDYIYRKRAEWITGGKIDEEWEEYL
                     AELERVGLEEWIQIKQTGYDNFLKGAQ"
     gene            8206..9690
                     /locus_tag="IWM35_002254"
     CDS             8206..9690
                     /locus_tag="IWM35_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016621147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HAP9920089.1"
                     /translation="MLVEETAILDKANRFIEMMRGKVNPQYRQNYHFSAPIGWINDPN
                     GFIYYKGHYHLFYQYYPYDSRWGPMHWGHARSSDLVHWEDLPVALAPDQPYDSGGCFS
                     GSAIERDGKLYLFYTGCQEIDGISHQHQCLAVSEDGIHFSKYSGNPVVAEKQLGENGL
                     IQEFRDPKVIVHNDGYYMVVATKSTQSTGRIVMFQSSDLYTWHFYSVLLEGTSEQGIM
                     WECPDLFHLDGRDVLIMSPIQIKKNGLEYHNISSTMACIGEVNWETGQMIVDNFHEMD
                     FGMDFYAPQTTRDPNGGRVMIAWMQMWDRTLPTHELGHLWAGSMSLPRELRVKKNRLV
                     QKPISTVYSHLVYQQGSENIAVTDTSVVFRNALSDSSYLHLVADLEKAEVFTIELLRS
                     KTDCLKLAYDTATQVFELDRQKLGRELTGAEEDPLNVRKVEVPLIDNQLVLEIFRDTS
                     SIEVFINGLEVMTATFYELEKARDILFHSIGESRIQAFETASVK"
     gene            9867..10859
                     /locus_tag="IWM35_002255"
     CDS             9867..10859
                     /locus_tag="IWM35_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005230454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP9920090.1"
                     /translation="MKATMKDVAKLAGVGVGTVSRVLNNGSVKASTREKVEAAMTELN
                     YQPDFYARGLKMKHTNTVALILPTVWHPFFGEFAYHVETALERQNYKLYLCNSSGNSE
                     KEQEYIQMVSQNKVDGIIGITYSDIDEYISSNLPFVSIDRYFTEEVIYVTADNAAGGR
                     LAANELYRRGCQNVAYIGGYQETPNETKSRRKYFEIECRQLGISYKALDMLEPLDHLS
                     ERVTNFLLENPDIDGIFTINDPMALAVIDMLEKIGKNVPEDVQVIGFDGQKMTEGDPY
                     LVSTIAQPTKQMAEVAVAKLIAVINGETVEKRTVLPIEFGEGDTTKPLVRNDFA"
     gene            10911..11780
                     /locus_tag="IWM35_002256"
     CDS             10911..11780
                     /locus_tag="IWM35_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP9920091.1"
                     /translation="MLYGGIEAGGTKFVCGIGNEQLQVIERKSFPTTTPEETMEQVFD
                     FFDKYKGNIASIGVGAFGPIDIQIESRTYGYITNTPKLAWQNYHFVGALENALSVPIY
                     WTTDVNAACYGEYMAGYGKGKNSVLYYTVGTGVGGGAVVDGKIVAGFSHPEMGHMLLQ
                     RHNEDQFEGICPFHHDCLEGLASGPAIEKRFGRKAQTISFDHPYWTIEADYLAQCVYN
                     TTLLYAPEVIILGGGVMKQPQLLELIRQSFKEKMGSYVDTPTLEEYLLTPRLADNAGL
                     IGCLALAIKKLNH"
     gene            complement(12200..13765)
                     /gene="guaA"
                     /locus_tag="IWM35_002257"
     CDS             complement(12200..13765)
                     /gene="guaA"
                     /locus_tag="IWM35_002257"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HAP9920092.1"
                     /translation="MSSPTRFDEEPKIIVLDYGSQYNQLITRRIREFGVFSELMSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDEKAFGIDEKIFDLGIPVLGICYGMQLITYKLGGK
                     VKPAKNREYGKAELEITSAEAALFQNTPEKQTVWMSHGDLVTQAPTDFDVVATSKDCP
                     IASIQNAERKMYGIQFHAEVRHSEYGNDLLRHFTLDVCQCTGDWTMDNFIDMEIAKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTCIFVDHVLLRKGEAEQVMESLGGK
                     FGLNIIKVDAKERFLSKLAGVSDPEEKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFKLIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGELTEEKLEIVRESDAILREEIANAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(13820..>14188)
                     /locus_tag="IWM35_002258"
     CDS             complement(13820..>14188)
                     /locus_tag="IWM35_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP9920093.1"
                     /translation="FKRPISQTEIVDELLSYNEELRTAYHFCQLLRYYFVKRGTEGFQ
                     ETLKTISSTLPQSFKKKFTIFHRYQSGISNAFKVSHSNGVTEGLNNKIKLIKRIAFGY
                     RNFYNFRARIYLQQGLIFEN"
BASE COUNT         4344 a         2405 c         2927 g         4512 t
ORIGIN      
        1 ttgaaaaacg ttgatttatc ggcattcccc atccatatta tatttacgtc ttattcccac
       61 tcaatatgag tagcctcgta aagaagctat tatatcagtg ctttttcagc ctattcctca
      121 ttttggtagg gaaatggtag ggttagattt ttactgcttg atttccatta aagtttgcaa
      181 ctttagattt tgttgcacag gtaaatttaa tgtttcctgt catttgttct gcaattcctt
      241 catcattacg gctccataga tgagcgtagt gctttaaagt gatttcaggg ctagaatggc
      301 ctaaccgttg cgacacgacc agaatgtctg cgttaaactc attgattagg tagctaacat
      361 gagaatgacg taatcctttt ccttgtatta ggggtacctt agctagtttg gcataacgct
      421 gaataattcg ttgaacagtt gaacgataga gtgggaggtc agtgtagcta agaataaatt
      481 gttctacacc atgatttttt tgaataactt tccacttttt tagcacttca attgtatcat
      541 cgtccagaga aatgatccgc aagccgcttt cagtttttgt gtaaggtttc cgaataaaat
      601 ctttttgatt tgtcatttcc aaggtgtgat gaaccctcaa ctttttacgt ttgaagtcca
      661 cgtcattcca attaagtgct aatccttcgc ttacacgaac accagtcatg taatatagcc
      721 agatcattaa aaaacacata tgttcgtaga aatcatcttt ataaattttt gccaaaactt
      781 cttcaaattc ttcttttgtc cagtaaggaa cgattgattt tccttttgga attgcttttg
      841 ttcttttgga aatattctca ttcagaaatt gcagggtgac cgcatagtct aatgatttac
      901 gaaacatacc gtacataagt gaactgtatg cttgtgagta tccgctatcg ttcaataacc
      961 aaattcgata attttcacaa tctcgcacat tgatttcacg aagcttctta tcaccgaaaa
     1021 attcagtaat ctgaatcaat ccacactcac gagaactcca agtacttttt tgtacgctag
     1081 ctttgtagtg tggaacgtaa gactgctcca taaattcttt gtaagttaaa tcataattcg
     1141 caaatccatt tacaaccatg tattcatttt ttatacgcat gacttctttg tgtgcttcac
     1201 gagctgatga gaatcgtttt cctagagaat cttttgttcc tttcttctgg atcctctttc
     1261 cagtcacttg atctgttcct aactcaacta gataagaaaa gcgaccattt ttatcttgat
     1321 acacacaagg gtattttgtt ttcatagtta ttcctcctgt gaaagaggaa cacctataat
     1381 ttcttcaatt acttgtcggg gtactacccc aactctttta ttattataat atggatatcc
     1441 ctgatttacc attcttgttt ttgcttgtcg gattattgtg gtagcttgat gtgttttaaa
     1501 tcctagagcc tctaaatctt ttcgacaaat tgtatttttc attgtataaa ctcactttca
     1561 aagaaaacac tatcattggg taatagtgtt atttctttat ttttgtataa tgaacggtac
     1621 tttcttgaaa atccatcact tgttcaggac ttcccaactc atttaaagct aaagcattgt
     1681 aaaaaaatgg acgtctaact atatattcgc ttgtatatag tatatcttga ttcgtggaat
     1741 catagggttt ctttaccatc tttttggtga cagttggtag tttgagattc cgggacttga
     1801 aaaaagcttt tttcttatgc tctttcaatt ccagctcttt atcgaaatat ttgctgatat
     1861 agcgcccgcg attttcgata ctgtctacat cgatcttgtt gatcttaacg aaaccctgac
     1921 cccaaatcgt agttaaccgg ttaaatgaga taaaggggaa atcaaacaag ataatatggt
     1981 agtggattgc accgcgttgc tgcttttccc acgtggcgag atactttaaa tttgcttttt
     2041 tagttttata gaggtcataa ttcagtcgtt tgataaattt tttaaattca ttatttgcat
     2101 actcaatgtc ttgaatgttt tctttgaaag tgagagttaa aaatttcgtt tgattgtcaa
     2161 aattcgtgtc tacaaggcgg gcaatctcaa atcgcatact ttgataatgt ttctgtttgc
     2221 gttttaaact gtcatattgt ccttgagcgg ataattcatc atagtttttt cttttttcaa
     2281 gagtaatatc attttcataa atccaagcta atttttgttt tcgttccaga tttacttcgt
     2341 tttcgatcgt tttacgtttg ggagtaatga ttgcttcata atcataaatt tcaatatacg
     2401 tgggagtttc aatgattttc tgattataag cttgcaaaaa tcatcactcc ttgttgcgta
     2461 ttcgattgtc aaagaaccag tcattttctc ctgatgttga tctataaatc aagttaaggg
     2521 ggaaggcctg cgtagcagcc cttctagcct atggggtgtt gggcggtctt aacggacaga
     2581 aacggctctg tgggcgctgc cccgccgttt cgtcctaacc acccaacact ccatagtata
     2641 gttagttttg tgtagtggta ccttgttttc tgggaatcaa agttgccgta tcagcggttg
     2701 ccgataaccc ataactgaca aattgtccat tcgagcgtcc ccaaggtaca atcaccaaat
     2761 tggtaaatgt aattatggct aaaccttgtt gggtaagttc ttccggcgtg atcacaggat
     2821 gttcagattt gactttgaca ctgatttttt cgtagcgaag ttttggtagg atacattcgt
     2881 agctccaacc tagaatttcg ccctcctttt gccagcgagt gacattgacg acttggtatt
     2941 ctttttcgag tgtttctttt ggtaaactga gatcggtaat ttttattggc atcacaattc
     3001 tcctttttta tacgaaagtt tatacgattt gattaaaaaa tatagatggc catctacgac
     3061 ttgtattata tacgagaact tatacgtagt caagagattt tctaaaaaaa tatatacgat
     3121 tttttatacg agtgttataa tgaaaaaaat gataaggagg aatttgaata tgtttggttt
     3181 agtacaacct aataaagtga aggtgggcca acgaatcaaa gaaataaaag acggaatgtc
     3241 tctctctttt acagaattag ggaatcgatt aggtttaaaa aagccgacga tcagttccta
     3301 cgttcaaggc tatgcgttgg ctcctaagag tgtgatcaac caattatcta gtatcagcgg
     3361 aaaacctgtc ggctggtttt attttgggca tgtggaagaa tatatccgag agtatttgtt
     3421 tctcattggt cagaaaaaaa tagtggacga ttatcctgaa atcattgaga aaataaagca
     3481 agaattttat acgggcgagt tcaaaaatcc tgcttgggag aatgagctag gctatccttt
     3541 ggaggaattt atccaagaat gttttgtaga atattcgagt gaaatcatga aggactatgt
     3601 cgtacagatt acgaaagagg tacttgaaga atccaaaacg ctaaacaaac tatcatcaaa
     3661 agcaaaagaa gaagctgtca cgattctttc atctgggatc attgactata tcgagatgag
     3721 tggtgagatc aagtatggag agaaagatcg cattgcgaaa atggtgaaag aatcaataaa
     3781 aacctttgat gtgaaacaag acctgacatt taatgattcc tatttgattg gaaagttgat
     3841 caatattctt ggtacagatg aagatacgga gctgtttctt tcttctttat caatgatatt
     3901 tactgataag catttttcca ctcgatttgg tggggaagaa ttagtcgaaa tatttcaatc
     3961 gatgcgacca gcgttaatca aactctatcg ggagaaaaca ccagatgaat tttatgattg
     4021 gtttgagaag taaaataaga aaagtggctt agattacaag ttggtgaaaa aatttttttc
     4081 agcataacat cagtaatcta taaaggggtt gttgattata ctgtagaagt taagtactca
     4141 agagattttt tgtacgtaaa caaagcaaaa aagggtgcag tttcactaga aaatgtactc
     4201 ttttttgtga tttttcttag ttatagagtt tttttgaaac tttaaaaatg agaaaacata
     4261 ttgattttaa agagtgtaga catagcttct tttgctaaat agattaaact tactggtgag
     4321 tggtcttgaa catactttta aatcacattt tactgagttt tattggattt tataattcat
     4381 tggaaacctc gtacttaatc tgtttttatc tatcgactag aatctgtata tattccagct
     4441 atcaatgagg ccgcagaaaa gggtgcttaa aaataatctg ttgtttaaaa tattattatc
     4501 agaatactta gatacaaaat tctggcacaa agtaaaaaaa atattgactt aaaaaaatgg
     4561 atatggtaat ctataaacga aaatagtggt aacgtttcca aaatgcatct tcgttaaatt
     4621 tttatttttt taaacgaaaa tggaaacgtt tctaaaatgt attgggagga gattgaattg
     4681 gaaaagacat tgggaccagg tggtgagata agggcggtac aacgggataa aaaaaatatt
     4741 tcgaagtggg aaaaattaaa gaaacagttg cggcgtgata agtggatgta cttgttgctc
     4801 ttaccaggta tcatcatgac gtttatcttt aagtatgtgc caatgtacgg tgctgtgatt
     4861 gcatttaaag actataatcc tatgctagga attttggaaa gtccttgggt aggactagat
     4921 cactttaggg aatttatcag ttcaccaaat tttacgcttt tgctgagcaa cacattaaaa
     4981 attagtattt atggtttatt gttaggtttt tttccaccaa ttattttagc actgagcttg
     5041 aatcaaatta tcaataaaaa aataaaacag aaaatccagt tgattctata tgcgccgaat
     5101 tttatctcag tagttattat tgtggggatg ctgtttttgt tcctatccgc aaatggacct
     5161 attaatgggt tagtagaaaa ggttacgggg caaccaatta tgttcatgtc taatccagct
     5221 tatttcagaa cgatttatat tttatcggga atttggcaag gaatcggctg gtcatccatt
     5281 ctttacaccg caacgctggc gggagtcagt accgatttat atgaggcagc aaatatcgat
     5341 ggggcaaata ttttacaaaa gatttggcat atcgatttgc ctgcattgag gcctgtgatg
     5401 gtgatcagct tcatcttggc cgccggcggt attatgaatg taggatatga aaaagcctac
     5461 ttgatgcaaa cttcaatgaa catccctgcc tcagaaatca tttccactta tgtatacaaa
     5521 gtcggcttgc aatcaggtga ttatgcctac tctgctgctg ttggtctgtt caatatgatt
     5581 attaatgtcg ttttattact gattgtcaac aatataacca agcggctaaa cgatggtcaa
     5641 ggcttgtaat gaaaggagaa aattatgaat agtgctttat caaaaatcga ccgtcaaatc
     5701 ttatggttga atcgagtgat tgtaactttt ttagtcgtca tcaccattgt tccgctgatt
     5761 tatgtactgg tagcttcttt tatggaccca gctactttga tcaataaagg tataagcttc
     5821 aatccgaaag attggacaat acagggatat caacgggtat ttgcagatga ttccatttta
     5881 cgcggttttg taaattcgtt gttttactct tttagcttca gcttattgac tgtatttatt
     5941 acgatgatca ctgcgtatcc tttgtctcga cctaacttgg cggggaaaaa gattattatg
     6001 actttttttg tcattacaat gttcgtgggt ggtggcttga tacctaccta tttattggta
     6061 aagaacttgg gcatgttaga tagtgtatgg gcgattatcg taccaggtgc tataaatgtg
     6121 tggaatatta tcttagcccg tacctatttt agtgctttgc caaaagaact ttctgaagct
     6181 gctactatcg atggcgccaa tgacttgcaa atactgttca aagtcatgct tccattagcg
     6241 aaaccaatta tgttcgtatt gtttttatat gcctttgttg ggcaatggaa ctcatacttt
     6301 gatgccatga tttacatcaa agatcccgac ttacagccgt tgcaactcgt attaagaaat
     6361 attttgatcc aaaaccaacc ttcacaagac atggtgggag ccaatactgc catggcagaa
     6421 atgaaacaaa ttgctgagtt gatcaaatac gcaacgatcg tcatttctag tctgccatta
     6481 atcataatgt atcctttctt ccaaaaatat tttgataaag gtgttttagt tggttctgtt
     6541 aaagggtaag tattgttcac tatttgtatg aataaaaatt ataaaggaga ttttaagatg
     6601 aaagcaaaat ggatgctgcc aatcgttttt acttccgtac taattttggg ggcttgtgga
     6661 ggctcaaaag aaaagagtaa atcaagccct gattatgaac tagaaaatgt ctcttttcca
     6721 ttgaaagaaa aagtatcttt aaaaatgatg tctcaaagtg cccctttggc tcccaatgat
     6781 cctaatgaca aattgatttt ccaacgatta gaagaagaga ccaatctcca tattgattgg
     6841 acaaactaca gtagtgattt tgccgaaaaa agaaatttgg atatcgctag tggtgattta
     6901 ccagatgcca tcttcaacgc cgctgcaggt gactatgatt tattaaactg ggcggaaagt
     6961 ggcgttattg taccgattga agatttgatc gaagattata tgccaaatct gaagaaaatt
     7021 tttgatgaaa accccgaata ccgacaaatg tctactgcac ctgatggtca catttactct
     7081 atgccttgga ttgaagaatt aggcgaagga aaagagtcga ttcatacagt aaatgggatg
     7141 gcttggatca acaaggagtg gttggataat ttgggtcttg aaatgccgca aaccactgat
     7201 cagttgatga ctgtgttgga ggctttcaag acgcaagatc caaatggtaa tggtgaagca
     7261 gatgaaattc cttttagctt tattaacaat ggaggaaacg aagacttaaa atttttaatg
     7321 ggggcattcg gtctaggtga taatgatgac cacttagtcg ttgataacga tggtcaggta
     7381 caatttactg ctaacaaaga agggtataaa gaagccatca aatatttcaa tgaaatgaat
     7441 aataaaggtc ttattgataa agaggctttc gaacaagatt ggaatacgta tgttgccaaa
     7501 gggaaagaac ataagtatgg cgtgtacttt acttgggata aagcaaatat cacgggtgca
     7561 gatgatagtt atgacgtctt gccagtctta gcgggaccag atggaacgaa acatgttact
     7621 cgtacgaatg gcatgggttt ctcaagagat cgtttcgtaa ttactagtgc gaataaaaac
     7681 ttggaactta cagctaggtg gatagataaa atgtatgaac cgttacagtc tgtccaaaat
     7741 aactggggaa cctacggtga tgaagcacag gcgaatattt ttgaattaaa ggatggtatg
     7801 ttaaaacatt tgcctttaga ggggacagca ccgggggaac taagacaaaa aaccgaagct
     7861 ggaggaccat tagcagttct tgattcttat tacggcacag tcactactat gccagatgat
     7921 gcgaagtggc gtttagattt gatgcatgac acgtatttgc catatattac agaagacaat
     7981 atctatccac gtgtcttttt agaaaaagaa gaaacagatc gattagcaaa aatcgaaacg
     8041 gatatgtggg attacatcta tcgcaaaaga gcagagtgga tcaccggcgg taaaatcgat
     8101 gaagaatggg aagaatacct tgctgaattg gaacgtgtag gtttggaaga atggattcaa
     8161 ataaaacaaa ctggctatga taattttttg aagggagcac aataagtgtt agtagaagaa
     8221 acggcgattt tggataaagc aaatcgattt attgaaatga tgcgaggaaa agttaaccct
     8281 cagtatcgac aaaactatca tttttccgca cccatcgggt ggataaatga ccccaatggg
     8341 tttatatatt acaaagggca ttaccatctc ttctatcaat actatcctta cgatagtcga
     8401 tgggggccca tgcattgggg gcatgcgcgc agtagtgatt tggtccattg ggaagatcta
     8461 ccagtagcgt tagccccaga tcaaccttat gacagcggcg ggtgtttctc aggaagtgca
     8521 attgaaagag acggaaaact atatttattt tatacaggct gtcaagaaat tgatggtatt
     8581 agccatcagc atcaatgttt ggcggtctct gaagatggta ttcattttag caaatacagt
     8641 ggtaatcctg tggtagcaga aaaacagcta ggtgaaaatg ggctaataca agaatttcgt
     8701 gatcctaaag taattgttca caacgatggt tactatatgg tagtagctac taaatctaca
     8761 cagtctactg gtagaatagt gatgtttcaa tcgtcagatt tgtatacttg gcacttttac
     8821 tctgtgctgc tagaagggac ttcagagcaa ggaataatgt gggaatgtcc tgatttattc
     8881 catttagacg gacgagatgt cttaatcatg tctccgatac aaatcaagaa gaacggactg
     8941 gaatatcata atatcagttc taccatggct tgtattggtg aggtaaactg ggaaacaggt
     9001 cagatgatag ttgataattt ccatgaaatg gattttggaa tggattttta tgcaccacaa
     9061 acaacaagag atcctaatgg tggacgggtg atgattgctt ggatgcagat gtgggatcgt
     9121 acattgccta cacatgagtt aggccatctt tgggcaggct ctatgagttt gccccgagag
     9181 ttaagagtga aaaaaaatcg attggtacaa aaacccatct caactgtata tagtcattta
     9241 gtttatcaac aaggcagtga aaatattgca gtgactgaca catcagtcgt atttcgaaac
     9301 gcgctgtctg attcttcata tttacatcta gtcgcagatt tggagaaagc tgaagttttt
     9361 acaattgaat tactgcggtc taagacggat tgtctcaaac tagcctatga cactgctaca
     9421 caggtttttg aattggatcg tcagaaacta ggaagagagc ttacaggggc ggaagaagac
     9481 cctctgaatg tacgaaaagt tgaagtacct ttgattgata atcagctcgt tcttgagatt
     9541 tttagagata cctcatcaat tgaagtcttt attaatgggt tagaagttat gacggctact
     9601 ttctatgagc tggaaaaagc tcgagatatc ttgtttcatt cgatcggaga aagtcgtatt
     9661 caagcttttg aaacagcgag tgtgaaataa atgtttgatt gcttaggcga tgaatcaatt
     9721 atttaatagg ttttgaagga taatgttttt agaaaagaac agatagctag atttcccatg
     9781 gatttgctat tagtattttg aaaaaattag taatttatga caaaattcat tatactaaac
     9841 ttgtgcagaa atgtgaggga tcaactatga aagcaacaat gaaagatgtc gcaaaattag
     9901 caggtgttgg agtaggaact gtttcccggg tattgaataa cggttctgtt aaagcttcta
     9961 ctagagaaaa agttgaagca gcaatgacag agttaaacta tcaaccagat ttttatgccc
    10021 gggggttaaa gatgaagcat acgaacacgg ttgctttgat tctacccacg gtatggcatc
    10081 cattttttgg cgaatttgcc taccatgtag aaacagcatt agaaagacaa aactacaagc
    10141 tttatttgtg taactccagt ggtaatagtg aaaaagaaca agaatatatc cagatggtca
    10201 gccaaaataa agtagacggc atcattggaa ttacttattc agatatcgat gaatacattt
    10261 cgagtaatct gccttttgtt agtattgacc gttatttcac tgaagaggtg atttatgtga
    10321 ccgctgacaa tgcggccggt ggacgtttgg ctgcaaatga actttatcgc cgaggatgtc
    10381 agaatgtagc atatatcggt gggtaccaag aaacaccgaa tgaaacgaag agtcgtcgaa
    10441 aatattttga aatcgagtgc cgtcaactgg ggatttccta taaagcgtta gatatgttag
    10501 aacctttaga ccatctctca gaacgtgtaa caaatttttt gttagagaat cccgatatcg
    10561 atgggatttt tacgatcaac gatccaatgg cattagctgt cattgatatg cttgaaaaaa
    10621 tagggaaaaa cgtaccagag gatgtccaag ttatcggatt tgatggtcaa aagatgacag
    10681 aaggagatcc ttatctagtc tcaacgattg cccaacctac aaagcaaatg gcggaagtgg
    10741 cagtggcaaa attaattgct gtcattaatg gagagacagt agaaaaacgt accgtattgc
    10801 cgattgaatt tggcgaaggt gacacaacga aacctttagt gagaaacgat tttgcttagt
    10861 gcaaatacgt ttcttttttt gaaaatgagt gaatgaaagg tgtagataaa atgttatatg
    10921 gtggaataga agccggtgga acaaagtttg tctgtgggat tggtaatgaa caattacaag
    10981 tgatagaacg gaaaagtttt cctaccacaa caccagaaga aacgatggag caagtgtttg
    11041 atttctttga taagtacaaa ggaaatatcg catcaattgg tgtaggagca tttgggccta
    11101 ttgatattca aatcgaatca agaacttacg gatatatcac caacacaccg aagttagctt
    11161 ggcaaaatta tcattttgta ggagctttgg aaaacgcctt gtcggttccc atctattgga
    11221 ctaccgatgt aaatgctgct tgttatggag aatatatggc agggtatgga aaaggtaaaa
    11281 atagtgttct gtattacacc gttggtacag gtgttggtgg tggtgctgta gtggatggga
    11341 aaatagtcgc aggttttagt catcctgaaa tgggacatat gctcttacaa cgtcataatg
    11401 aagatcaatt tgaaggaatc tgtccttttc atcatgattg tttggaaggg ttagcatcag
    11461 gacctgcgat tgaaaaacgc tttggaagaa aagcccagac tatctcattt gatcatcctt
    11521 attggacgat tgaggcagat tatcttgctc agtgtgtgta taacacaacg ttactatatg
    11581 caccggaagt aataatttta ggcgggggtg taatgaaaca gccgcaacta ctcgagttga
    11641 ttcgccaatc gtttaaagaa aaaatgggtt cctacgtgga tacgcctact ctcgaagaat
    11701 atctactaac tccaaggctt gctgacaatg cgggcttgat tggctgccta gctttagcaa
    11761 taaagaaact caatcattag aatagtattt attagaacta ttcataacat accggaagtt
    11821 taaaagggta ttctgaaact taaatagtct caattcattc ttctttcttg agaatctgag
    11881 tagagacttt tattctgttt caaattaata taattacgaa atgatattga gtacaagaaa
    11941 gttcttttta ccagatcttt taaatcgaaa aatctgatat atatatttat agtactaaaa
    12001 atctgattag ataaaaagct aattcaatag gaaaataaag gtagtgaata aaagcaaagc
    12061 ccttgttagt agttaatcta acaagggctt tggtagggaa aatggtagga aaccatctta
    12121 ttttttacga aaatcaggaa ctttagtaat tgaataatgt tgatttatag gtattcctca
    12181 tccatattat gttggctctt tattcccact ccactgttgc aggtggtttg ctggtaatat
    12241 catacacgat acggttaacg tgtgcgactt catttacgat acggacactg attttttgca
    12301 agacttccca agggatacgg gcaaagtcag cggtcatgcc gtcaatagaa gtgacagcac
    12361 ggatacctac agtgtagtca tacgtacggc catctcccat aaccccaaca gagcgaatgc
    12421 ctggtaggac ggtgaagtat tgccagatgt cgcgatctaa accggcattg gcgatttctt
    12481 cgcgcaagat agcgtctgat tcacggacga tttctaattt ttcttctgtc aattcaccaa
    12541 ggacacgaat acctaagcct ggtccaggga atggttggcg ccaaacgatg ctgtcaggca
    12601 tgcctaattg ggtacctaat tcacgtactt cgtctttgaa caatgtgttc aatggctcga
    12661 tcagcttgaa ttgcatgtct tcaggtaatc cacctacatt gtgatgtgat ttgatggttt
    12721 gggcagtttc agttcctgat tcgatcacat cggtataaag ggttccttgt gcaaggaaag
    12781 aaacgccttc ttcaccggca agtttggttg cttcatcatc gaagacatat acaaattcat
    12841 ttccaatgat tttacgtttt tcttctggat cagacacgcc agcaagtttt gataagaaac
    12901 gttctttggc atcgactttt ataatgttca agccaaattt tccgccaaga ctttccataa
    12961 cttgttctgc ttcaccttta cgcaacaata cgtgatcaac gaagatacat gttaattgat
    13021 cgccgatcgc tttttgtaaa agaacaccaa caacagagga atctactccg cctgaaaggc
    13081 ctaataaaac ttttttgtca ccgacttgtt cacgaatttt tgcgatctcc atgtcgatga
    13141 agttatccat tgtccagtca ccagtacatt ggcagacatc tagtgtaaaa tggcgcaaca
    13201 agtcatttcc atattcggaa tggcgaactt ctgcatggaa ttggattccg tacattttgc
    13261 gttctgcatt ttggatcgat gcgatggggc aatctttgct ggtagcaacg acatcgaaat
    13321 cagtcggtgc ttgggtaact aggtcaccat gactcatcca aacggtttgt ttttcaggag
    13381 tgttttggaa aagggccgct tctgcagaag tgatttccaa ttctgctttt ccgtattcac
    13441 ggtttttggc tggtttaact tttccgccta atttataagt aatcaactgc ataccgtaac
    13501 agattcctaa aactgggatc cccaaatcaa aaatcttttc atcaatacca aaggccttct
    13561 catcataaac actgtttggt ccgccagaaa ggatgattcc tttagggttc attgccttta
    13621 cttcttctgc ggtaatgcgg tggctcatca actctgagaa aacaccgaac tcacgaatac
    13681 ggcgagtgat cagttggtta tactgactac cgtaatcaag tacgataatt tttggctctt
    13741 cgtcaaatcg tgttggtgaa gacaaatttg aataaaactt agtccgatat gcggcgcgtt
    13801 ctgtgcgccg catatttttt tagttttcaa agatcagacc ttgttgtaaa tagatccgtg
    13861 cccgaaagtt ataaaagttg cgataaccaa acgcaattcg tttaatcagc ttgatcttgt
    13921 tattcagccc ctcagtgacg ccgtttgagt gggacacctt aaaggcattt gaaatacccg
    13981 attggtagcg atggaagatc gtgaatttct ttttaaacga ctgaggtaac gtagatgaaa
    14041 ttgttttaag ggtttcttga aagccttccg tacctcgttt aacgaaataa tagcgtaata
    14101 attgacaaaa atgatacgca gttcgcaatt cttcgttgta gcttaacagc tcatcaacga
    14161 tttctgtttg actaattggt cgtttaaa
//
LOCUS       DACDXO010000068        13943 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E23605_2017
            SAMN12639989-rid9881463.denovo.069, whole genome shotgun sequence.
ACCESSION   DACDXO010000068 DACDXO010000000
VERSION     DACDXO010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639989
            Sequence Read Archive: SRR10023389
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741914.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:42:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,972
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 33 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 17 of 73
            Pseudo Genes (multiple problems)  :: 10 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13943
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639989-rid9881463.denovo.069"
                     /isolate="E23605_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            949..1422
                     /locus_tag="IWM35_002259"
     CDS             949..1422
                     /locus_tag="IWM35_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HAP9920094.1"
                     /translation="MKFKTLLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTT
                     LFVSVIYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGL
                     LPKFMKVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            1431..1658
                     /locus_tag="IWM35_002260"
     CDS             1431..1658
                     /locus_tag="IWM35_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9920095.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
     gene            2294..2665
                     /locus_tag="IWM35_002261"
     CDS             2294..2665
                     /locus_tag="IWM35_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9920096.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
     gene            complement(2921..3163)
                     /locus_tag="IWM35_002262"
     CDS             complement(2921..3163)
                     /locus_tag="IWM35_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAP9920097.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
     gene            complement(3195..4097)
                     /locus_tag="IWM35_002263"
     CDS             complement(3195..4097)
                     /locus_tag="IWM35_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAP9920098.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            complement(4110..4298)
                     /locus_tag="IWM35_002264"
     CDS             complement(4110..4298)
                     /locus_tag="IWM35_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HAP9920099.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            complement(4312..4875)
                     /gene="amaP"
                     /locus_tag="IWM35_002265"
     CDS             complement(4312..4875)
                     /gene="amaP"
                     /locus_tag="IWM35_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HAP9920100.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            complement(4913..5806)
                     /locus_tag="IWM35_002266"
     CDS             complement(4913..5806)
                     /locus_tag="IWM35_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP9920101.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKDAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            complement(5884..6822)
                     /locus_tag="IWM35_002267"
     CDS             complement(5884..6822)
                     /locus_tag="IWM35_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAP9920102.1"
                     /translation="MRYYFVDEEKNVIETDEKKYNWLVLDSAKPEEIEQAIEEFELPD
                     DIFVGTEYPEEVSRMERLYETKLEHPFSLVVINLDQSDNRIEKKLTPISFVLSDNLLI
                     TCIDRKTEFVDRLLTKHGNHLNSFEKIITYTLLDIYTHYVKELREMKKRIDALDKAAR
                     KTTENEELYKQADLERDIVYIDHTLRDQRETMDQLWNSETFIKRLDDEQLLYDVHLRQ
                     RQTEKMIEIYRDLLESIGDLFNGMMDNNLNHIMKYLDSAALIISVPALFAGIWGMNTG
                     GLPGKSSALGFILVVFGSLIAAVVLGYHLYRKDYTN"
     gene            complement(6856..7206)
                     /locus_tag="IWM35_002268"
     CDS             complement(6856..7206)
                     /locus_tag="IWM35_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAP9920103.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            complement(7239..8141)
                     /locus_tag="IWM35_002269"
     CDS             complement(7239..8141)
                     /locus_tag="IWM35_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HAP9920104.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            complement(8134..8991)
                     /locus_tag="IWM35_002270"
     CDS             complement(8134..8991)
                     /locus_tag="IWM35_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HAP9920105.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
     gene            9325..10134
                     /locus_tag="IWM35_002271"
     CDS             9325..10134
                     /locus_tag="IWM35_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP9920106.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIAMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
     gene            10174..10671
                     /locus_tag="IWM35_002272"
     CDS             10174..10671
                     /locus_tag="IWM35_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP9920107.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
     gene            complement(11056..11129)
                     /locus_tag="IWM35_002273"
     tRNA            complement(11056..11129)
                     /locus_tag="IWM35_002273"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11093..11095),aa:Ile,seq:cat)
     gene            11316..11639
                     /locus_tag="IWM35_002274"
     CDS             11316..11639
                     /locus_tag="IWM35_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9920108.1"
                     /translation="MLGVIILPFIAILFDLVAAAAYFLQLNYQTSGILFIGMIFQGVI
                     TLLLLVMMLSYKGKRYARIQTKVFVKYVSIRYGIIILSFLVNAIVLFLYVLNYLDINP
                     LIFSR"
     gene            11803..12057
                     /locus_tag="IWM35_002275"
     CDS             11803..12057
                     /locus_tag="IWM35_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9920109.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
     gene            12127..12372
                     /locus_tag="IWM35_002276"
     CDS             12127..12372
                     /locus_tag="IWM35_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9920110.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            12448..12813
                     /locus_tag="IWM35_002277"
     CDS             12448..12813
                     /locus_tag="IWM35_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9920111.1"
                     /translation="MRLWHEKLISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFH
                     YSPYRLFQYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHD
                     EVYLAECLANLEQKGIYLT"
     gene            complement(12874..13437)
                     /locus_tag="IWM35_002278"
     CDS             complement(12874..13437)
                     /locus_tag="IWM35_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9920112.1"
                     /translation="MLYIKKGIIVISFFKEYLEIINFRLKTKKESVIILVYFQGGNLM
                     IKKLFLLSILTLAMAGCSDSGLATELITKSTEATTEELKCGNDHSDYRGLNRRCMGIL
                     GRSNCKHRCSNRRRCRRSNRKIKRKIKRSNLCCASYTDVEDAVLLNHVRRCSFMSREV
                     GDEPKLEYAFSFAREDEDNPKTYDILA"
BASE COUNT         4087 a         2577 c         2304 g         4975 t
ORIGIN      
        1 ttttcggaga attaaaaaat acaataaata actttagtta ctttaatgcg ttcttacttt
       61 atttttatat tatcattttt cttattcact tctttggttt atactattct tataaataaa
      121 aacgaggtat gtcctatgat gaaaagattg tactattcat tgatcataac gattggctat
      181 ttgattgtat caaatctagg aaatatggta tttggtatca gtaaagaatt cagttggact
      241 actacattat gggaatctct attctttttc atcttcgtat ttttgttaca aaattatcga
      301 aaaaaataaa catgtgccat ctatctgttg gcacatgttt taggatttat ggtacttttt
      361 attcggcaat ccattttatt tttctacttc ttcttaatac ctctaacact tttgatttat
      421 aaactaatcc aatgcatcta cttgcgctta ttaagctatt tcagctagat agtaccaccc
      481 catgcgtcaa tgcttgattt tccttttgta taacattgta taagcaataa aggacaaata
      541 agtggtgatt acaccaaacc attgaataag aaaaattctg atacgtaaat aaacatgaac
      601 ttgaaaaaat taatgaatcg gttagctact acgtagttgt cttttttctc atacagttct
      661 gttaacttac acttctcttc gctagataat aaaaaatcct ccaactgtat tgtccaatat
      721 aatcccaggc atctaaacta gcattataga agagacacac atagaatcta aaataactgg
      781 ttttaaatgt ttcttacaat caaaaaaagc tgttaaatgc actccacacc ttcatactct
      841 atcatttttt ataaacgata tatttttatt gtttttcgat aaaaaatagt tgttttacaa
      901 taattattct gctatatttt agctatcaaa gataaaagag ggataaaaat gaaatttaaa
      961 acattgctat taaaatttgt cgttttaggt ataagtgccg tatttatttt gttcggcgta
     1021 cttttttata ctcagatcgc tacaagcgaa aaaggattca attttgattg gcagaccaca
     1081 ttatttgttt ctgtcattta ctttgccctt ctcctaggga tcgttattgc ttatttctta
     1141 ttgaaactat tgaaaaaaat cgaagataac caagcttttt ccactcaaag cttaaacata
     1201 ctaaaaaaaa tcgaacgaac cattttgttt attggtttcg ccttttttgg cttacttcct
     1261 aaatttatga aagtagcgga tttagacgat gctccaggtt tgatgttggt aggaatcgct
     1321 gtcgtattca ttccttttgc agtatacaca ttaacaagcg tgctggaaaa actgcttgaa
     1381 catgcgattt tacttaagac agatcatgat ttgacagtgt gaggtgcgac atggcaatta
     1441 tcgttaattt agatgtagta ttagctcaaa aaaaaatgag tgtgactgaa ttgagtgaga
     1501 aagtgggcat tacgatggca aacatctcta ttctaaaaaa tggaaaagca aaagccatcc
     1561 gtttttccac tctcgaaaaa atctgccaag ttttggagtg tcaaccaggt gatttgttga
     1621 cctataaaaa tgactcgaat actataccag aagaataaaa taaagtagaa caaacagtaa
     1681 ataaaaaata atctgaatga tagatgtgtg agacttttag aagttattta cgtctattat
     1741 tcagattttt tatttcgatt gaataccgtt ttatctataa ataagatatg tactgactca
     1801 tacaaagggg gccttttctt ctccatgtac tatcactttt cctgatacaa ctggtttcct
     1861 gttacgttgt agttggatct gcttttagta taatacttta ttcattccac cagactgata
     1921 gcagtttgag cagtctaaat aatcatgtag tattacaggc cattctgttt cataccttga
     1981 agtgcttaaa acttcagcta atggcccatt gtgttaactc ttactccatt cttttttagt
     2041 aagctcccat atatactgta aagccttcta tatattatgt catgttgctt tttaacattt
     2101 actatgtagt tttgataaaa tgatttgaat aacagtgatg gacaattctt ttttattttt
     2161 aacattattt tgataataat gattatttat ttaaaaatac attagataac atttgttacg
     2221 acacgaaaaa gcttcacaaa caaatatcgt tattttaaaa tgagggcgaa ctcacacaat
     2281 taggaggaat agtatgccat ttattgttta cttttttctt tcattactcg tgatctatat
     2341 tccattacca acatttttcc tgctcgtcaa tcgtctagca gagcaacatg atacgattgc
     2401 tgctcttacg aaactttgtt tacttctatt tgtttgtcta gattacttta cgagctatta
     2461 ttggctaaaa cagacaagag taaaaaagag atattatttc ttccttgtct ttttcaatct
     2521 ctttgagttt ctgatccatt gctttttact tcatcagtat catacgatcc atttgtggat
     2581 catctgggtt ttcaaagtgg tcgtattatt ttgtatgttc acctttccta tctcaagaaa
     2641 atttagggag gcattgtttt attaaagaag cttctctgta tcaataaaga cacatttaag
     2701 gaaaaacttt taaaaatcta attgggaatt tgcccgcata aataagaggc tgtgacaaaa
     2761 gtcttgtcac agcctcttat tccgtataaa cagtgttcaa aagcttgcat cttcgataat
     2821 atgcccaacc aaatgaaaaa tatgagaagc tatttttgtt tggctgttct tattacttga
     2881 tgaagaacag ctttttcacg acctcttttt aaaagtcttt ttacagtttt cgagcaaaga
     2941 atgatacgat tagtactaaa atgatggcac caatcaatga tggcacgatt gccattcctg
     3001 caatacttgg tccccaagaa cctaataatg cttgaccaag tgctgatcca actaaacctg
     3061 ctacgacatt tgcaataatc cccatcgaag aacctttatt cgtgattgct cctgcaattg
     3121 ctccgataat tcctccgacg ataagtgacc aaataaatcc catgtttcgt tcctcctaat
     3181 gttttacttc cttattactg aacccgtggt tctgtatttt cttttacttt ttgtgcgccg
     3241 cttccagctg ctgattttac tttatctgtt tgattcgaag caaattcacc ggtttttttc
     3301 gctgcgtcag ttactttgtc ttgcaccgtt tcttgatctt tttcaaattc ttcttttgtt
     3361 ttgatgtcca ctacgttagc gttgacttcg atcacttcta aatgtgtcat attttttact
     3421 tctttgccga ttacttcttg catttcttca aagatttttt cgatatcttt gccatattcc
     3481 acaatgacat ccatatcaac tgctacttgt tttttaccga cttctgtttc gatcccagcc
     3541 gttacattat ctgtattcac aagttttcct gccacattag agaagaaacc accgtttgca
     3601 gataataaac cgtctacttg ttcaattgcg attccgatga ttttttgcac aactttatct
     3661 tcataattca gctcaccgcg cattgaatct tcctgttcag aatcatgctt tccttggtta
     3721 tttgtatgat cagaaccaag ttttccttgt ccgtttgtgt ggtcagaact aagtttttct
     3781 tgtgctttcg catggtcaga aggatgtttt tgctcttcca caggtttaga aggatcgatt
     3841 gcatcatgtt gttctggaac tttagattct ggagttcctg gatgatgagg tgcatgcttt
     3901 tctgcatcat gtttcttctc ttctactcct tgttgaacgt tagggactac tggatctgga
     3961 tgtgcagata ctggtgctgt tttgttcttt tcatcgttca caccatttga ttggtttggt
     4021 gtcgttgggt ctggatgtgc agacactggt ttttggttgt tcattttatt gttattattt
     4081 ttgttattgt tattcatgga tgtttcctcc taagatttaa aatagtgatc taagattttc
     4141 gtttgtttca gataaaaacc gatagcgacg ccgatcgctg ccatgatcaa gatcaataag
     4201 gtcttgaaaa agcccacggt gatccataaa acggctaata tcaatccaag taccccaaag
     4261 atgataggga gcttgtatgt cgaaaatagt tctttcattt ttggtccccc cctattctac
     4321 tcgtttgcgg tcattgcttc gtttagctgg ttcttcaaag cctgtgtatt tcaccgatac
     4381 tttcactggc tcttttgttc ccaatatgtg ttgtacttca ttttcgattt ctttcatcaa
     4441 tgttcctgtt ttaccaatga gtgctgaagt tcgtttcaac tcccctttta cattgacggc
     4501 gatcttgttt ttcgttgctt tgacatttac ttttggtgaa tggatgaatt catcttcatg
     4561 caagtgagaa cgaaccattc cttcaattgc ttttttatcc aacgtcaact ccccatcacc
     4621 atgtttcagt agaaagtttc cgattgtttt tggataaaac aggaaaacaa gtaacgtgat
     4681 aattgtcaaa atagcaaatg caacggcaag ccaaaataca tattgttgca cgtatgaatt
     4741 agtgatggaa aagaatcgaa acttctctag ctgaaaaggc aatggatagg ttgcacttaa
     4801 tagtatcgca aacaaaagca cagatagcaa aagtaatgtt ccgatcactc cgatcgtttt
     4861 tacaccttta ttcattttcg cacctcctca taggggtgaa acgatcaact tcttataagt
     4921 tgatcgcttc tccaccagtt actccgtaaa tttgggctgt aacataactt gctttattcg
     4981 atgctaagaa aacataaact ggtgctaatt ctcctggctg acctgcacgt tctagtaaac
     5041 tttgctgacc aaattcaggg atcgaatcag aaggttgtcc ttcgtctagt tgtaatggtg
     5101 tccagattgg accaggtgct acaccattta cacggattcc tttaggtgca agctgttttg
     5161 ctaaaccgat cgtaaagtta gcgattgaag cttttgttgc tgcgtaatct aataaatgtt
     5221 cgcttggatt gaatgcttga acagaagttg ttgttacgat tgctcctcct gctgggatat
     5281 gcggaacagc ttctttcact aaagcaaaca tagcgatgat gtttatcata aatgtgtctt
     5341 tgacttgctc aagtggtaac tcagcaatag actgacaaga aatttgctgt cctgcgttta
     5401 gtaccatcgt atctagcccg ccaagttcac tcacggcttt atctatgatt tctttaggtg
     5461 cttgttcgtc acgaagatca aaaggtagta agacaatctt acgtcctgca tctttcacgt
     5521 attttgctac ttcctctgca tctttttctt ctcctgggaa gaattgaaga gcgatatctg
     5581 ccccttctct agcaaatgca atcgctgctg cacgaccgat ccctgaatct ccaccagtga
     5641 ttagtacacg gcggttttct agttgattat tgccttcata ggattcttct ccacaatcag
     5701 gaacaggaat catattctgc tgtaaagctg gtgtatcctg atcttgttta ggaaatttct
     5761 ccgtatggta caagtttcgc gggtcgatta attttggttc tgtcattgct gtattcctcc
     5821 tagtttttaa attgtattgc caaaacaaat ttctttgttt gatacctgtt ttaaatagat
     5881 tctttaattt gtataatctt tccggtataa atggtaacct aaaactactg ctgctatcaa
     5941 agagccaaag actacgagta taaagcctag agcagaactt ttacccggta atcctcctgt
     6001 attcattccc caaatccctg cgaacaacgc cggaacggaa atgattaatg cagctgaatc
     6061 tagatatttc atgatatgat ttagattatt gtccatcatc ccgttgaata gatcaccaat
     6121 actctctaat aaatctcgat agatctcgat cattttttct gtttgtcgct gtctcaaatg
     6181 gacatcatag agtagctgtt catcgtctaa acgtttgatg aaagtttcag aattccataa
     6241 ttgatccatt gtttcccgtt gatctcttaa ggtatgatcg atatatacga tgtctctttc
     6301 aagatctgcc tgtttataca gttcttcatt ttccgttgtt ttacgagctg ctttatccaa
     6361 agcgtcaatt cgctttttca tctctcttag ttctttcacg taatgggtgt aaatatctaa
     6421 taatgtatac gtgatgatct tttcaaacga gtttaaatga tttccatgct ttgtcagcag
     6481 ccgatccaca aattcggttt ttctatcgat acaagtaatc aagagattat cggaaagtac
     6541 aaaagatatt ggcgttaatt ttttttcgat acgattatct gactgatcta gattaatcac
     6601 taccaaagaa aatggatgct ctagtttcgt ctcataaagg cgctccatcc gagaaacttc
     6661 ttccggatat tcggttccta cgaaaatatc atcaggaagc tcgaattctt cgattgcttg
     6721 ctcaatctct tcgggctttg cactatcaag tacgagccaa ttatattttt tttcatctgt
     6781 ttctattaca ttcttttctt catccacaaa gtagtatctc aatgtatacc tcctttttgg
     6841 actttctcga aactattagc tgaagaagca gttctttaat ttgatattat aatcgagtac
     6901 tgcgatttca ttttctaatg aaaagctgtc aagctgtttg ttgaagtcca tgttcaatcg
     6961 tttaccgaaa taattttgct ctaatttaga tgtatagatg tccaataatg gatgctcgat
     7021 tttagtcgca tattctccaa attccgaaac aaaaggaatg atttgaaaaa agctgccttt
     7081 tgcaaaagca cctttgtccc ttagaaatgg atcggaatca ttgataagcg cgagtgcgat
     7141 ttttgtattt tccccaacct tttcaattaa cgtctctact gcatttttag acattgtaat
     7201 ttccattcct actccccctc ttcagttgtt gtcttctatt atttaacaac tacatctgtt
     7261 tttggttgaa cttttgcata ttttggatat ttagggaatt tagctacaca acggaaagcc
     7321 gtcatccctg catatttttc gtaatatttc ttcgttggcc cttcaaaatc cttgctccac
     7381 ggtttcacat gtcctgaaaa atggataatg taaggattgt tgcgagtttc ttcgtattca
     7441 cgttcacctg ctgctgttgg atgttttttt gcttttgcca tgatatagcc ttgtgcattc
     7501 caacgcggat gaagtggtaa ccaacggtca tgtaagatag catttaatgc atcttggtca
     7561 tggaatcgta atttttctgg attatgttcg atgaaatcta atactttttg cgtaatattt
     7621 tcatctaacc attttttcac attgatcagc atcaaacctg agttgaaata acgcattgat
     7681 ttagctgaaa ttttcatttt ttccagtcgt tgatggaacc cagcgtcttc aacagctgct
     7741 acgattgaat caccgaagtc aagacgccat aatttagaga tatcttgtaa agcgatcatg
     7801 tcgcaatcca tatataagat acgttcaact tctgtgccgc ggaacaattc cggaatcgca
     7861 atacgataat aagctgtttc tggaatacga tcgctgatta ccacatttgt aaagaattct
     7921 ttattgattt tcaagaattc cacatctgag ttcattcgtg cgtttttcac tgagaagcgt
     7981 aaaagctgtt tactttcaaa atcgatatca tcgtcaatca catagaaccg aacaaattta
     8041 gacgggttgc aattatctaa tactgagaca aataatgcag ctaaatgtgg tacgaatttt
     8101 gtgttacaac ttgcgactac tgctatttct tttttattca ttgatctcgc ctactttcgt
     8161 tagagtactg tgtgctaatt tttttaagta taaattggta tacggatgat ctttcaacgt
     8221 attccaaggt ttatcatgtc ctgtaaagtg aacgatcgaa ggtttttcat ttccttcttt
     8281 atactgacgt tcatattttt catttggtgc tggatgacgt tcaaaaatca gcgaagtctg
     8341 catgttccat tttggatgaa gttgttccca atcttcataa agtacagcat tcaaagcatc
     8401 ttggtcgtga tagatgattc gatcgccatt ttcactgagg taatggatag ttttttctgt
     8461 aatttctttt ttattccatt gatcaatgtc aatcaccatc actcctgaat tgaagtacaa
     8521 gtcatcggaa tcgattccta atctttccaa agctttcact tggcctggat cgataatagc
     8581 gccgattgtt tttccattta acggctcatc atatagtttt acgatatcgt ctagtactaa
     8641 cacatctgaa tctaagtaaa ggactttctt ataatcctct ttagctaata agtttggtaa
     8701 agaaattctt aagtaagcgg tagttgtgat atggtcactg actaggaagt cttcgtagat
     8761 atctttttcc actgtcagga attggatact tgcattactt gaatatttat ttaccgtttc
     8821 ttccaatcct tctttactat attctgatag accatcgtca atcacataaa attttatgcg
     8881 tcttgtttta ttgcaatttt ctaatgcagt tgcgatcatc acgctcaagt aaggagcata
     8941 attttcgtct gaagcagtta ccacaggaac tacgccgtat ctcttctcca tcaaaatcac
     9001 tcctttttct aatctttttc taacctgttc tacatctaga atattcctca tttggtttta
     9061 aagcaaaata tatgctttgt tttcattgaa tattttaaaa tttttttatc acaaaacgca
     9121 tcggtaagcg ctaactattc gtttttcatg gctttttatt caaaggtatt gaccaaaaac
     9181 tactatatat agccaccttt ttcttaagaa tgttttttga aaaaattcat aatgacaaag
     9241 caagttagtt tcattttttt aaattctcat ttaatcttaa ggtaacagga aaatagatat
     9301 gtaaaaagaa agtaggaaga taaaatgaaa ctagcagcga ttgatttaga cggtaccttg
     9361 cttgatcgaa atggaaagat accagaagaa aatatccaag cacttcggaa atttgatgaa
     9421 aatggcggaa tcgtaaccgt cgctaccgga aggaacagca tttctgctaa agatattttt
     9481 tccgaattag gaatcggtgg ctatttgatt tcttccaatg gttcactgat tgctgaaatg
     9541 aaggatggaa aaatggcgaa agttctcaga cgttcaaaaa tagatagcga tacactgaga
     9601 aaagccttct tctatgctca agaagcgaaa atatccatca ttgctagccg agaaacccaa
     9661 gatgatcagc tgacattcga tgaatcaaat ctagtcaaag atgaccctta ttatgaacat
     9721 ttcaatttac agaaccattc ttttgatcaa attttaaata tgttggaaga tccttctctt
     9781 agctatctta aattagcatt aacagataag agtgaagaaa aactacagaa gctacaaaga
     9841 gatctgcaag cagatggtat ccaatcagta ttctctgatc cgcatttctt agaaatcacg
     9901 cctacaggta tcactaaagc tcattcactt gtttttctgg cggattacct tggaatcgaa
     9961 tcgagtgaca tcatggcttt tggcgatcaa gaaaacgaca ttgctatgtt gaattacagc
    10021 gggttgagta ttgcgatggg gaatgcacag cgacaagtaa aagaaattgc aaaccaagta
    10081 acactgacga atgaagaagc tggtgttgct tatattttaa aaaacttcta ttaaaaagaa
    10141 taaaatgacg atatgaatgt aggaggaaag aaaatgttag ttattattga tatgcaaaat
    10201 catattcttg atccaaacag cgaattttat attccagagt cagatcaatt ggcagaacgt
    10261 attgctcaaa gattagctaa agcacgccaa aataatgaat atattctctt cacacgggat
    10321 ataccagttg aactgaagga caaagaagaa gaacgagaag atttgcaatt gatccctctt
    10381 ctttctccac aagaaaatga acgagtgatt aaaaagtatt actttacgat tccacctgaa
    10441 actttagctg aaatccagga aactttattt gaaagcaaag aagaacaaaa agagatcgaa
    10501 gtagtcggga ttgaaacaaa cttgtgtgta ctctctaatc tgataggcct gcagagtgct
    10561 tttcctgaag cagacttttt tgttgatcca atgttagtct ccagtagaaa acacggagaa
    10621 tccgctttag agctgttgaa agatttcaac gtctctatcg tcgaatcata aaaatatcta
    10681 tcttcattta cgatatttaa cacacaataa aaaaagcacg cagaactgtt ttagtcctac
    10741 gtgctttttc tatcaaagta gattggcttt tttatgacac agagtgaaac ggcaggctca
    10801 cgacctcttt cttaaatggt gttcaatcag ctgaccctcg gtattagctt aacaaacggc
    10861 aaaatatgag gaactatttt tccgtttgtt aagctaatac tcaggtcaaa acgctgattt
    10921 cacaacctct tccttaaacg gtattcaaaa gcttgcatct gcagtaataa gaacagccaa
    10981 gtaaaaacat aagaagctgt ttttacttgg ctgttcttat tacttgatgc agggcagctt
    11041 tttcacaacc tcttatgtac cttgtaggac tcgaacctac gaccggacgg ttatgagccg
    11101 tctgctctaa ccaactgagc taaaggtaca tcataaataa aatttatttt tgttactttt
    11161 caactatagc acgaatgaca aactctcaca agtttcaaaa tataagtaaa ataatattgt
    11221 tattcttatt tcttttactt gtgtcataac tattttgtta ctttaatttt tatttgctta
    11281 ttttagaaaa aatgttattc ttttatttat tctcaatgtt aggagtgatt attttgccat
    11341 ttattgcgat tttattcgat ttagttgcgg cagcagctta ttttctccaa ttaaattatc
    11401 aaacctcagg aattctgttt attggaatga tttttcaagg tgtgattacc cttttgcttc
    11461 ttgtcatgat gctcagctat aaaggaaaaa gatatgcccg tattcaaaca aaagtatttg
    11521 ttaaatatgt ttccattcgc tatgggatca tcattctttc ctttttagtg aatgcgatcg
    11581 tcctcttttt atatgtattg aattacttag atatcaatcc attgattttt tctcgataga
    11641 taagacacat tcagcggaac agttcctgct tatcagcaga aaactgttct gctttttcat
    11701 ttttcattga tctattcttc ttttattttc ctagcatttc atttgtgatg tatgcaataa
    11761 catgttatct tttattatat gaatcgttga ggagtccgtt acatgattac gttggtgaga
    11821 gttttgtttt gggtgcctgc ggtcgcactg gtcgcaagta ttgtttatct tatgaattgg
    11881 aacaaagaac ggttttattt agctattttg actcttccgg ctatctactt tatgtggaaa
    11941 gtttttaatt acaactactt tgaacccgat agtgttttta tagaagaatt atcaggactg
    12001 gttttgtctt tattgatcgt cattctttat ttgatacgtt taaataaaaa acattgagta
    12061 ctattgattg catgtgtagc tttctatagt cattataact attctactat aatgaaaggt
    12121 gattaaatga gtatcgaaga agctatttta ctggatactt tacgttgtcg tctgaatttc
    12181 ttaagaactg gccgatcttt atctcaagta gctgtttcta gcgcgttaag tattccttta
    12241 ttcaaatatc gtaagtacga agaaggagaa gaaattccaa gcatcattga attaatctat
    12301 atcgctgact tttacgatat ttccgttgat tacctaattg gtcgctcaga aacagcaagc
    12361 aaactgaagt gattttttcc agccttggtg ctggtttttt catttaacgt ctaaggttaa
    12421 caatctatat ataaaggaga catttctatg cgattatggc atgaaaaatt gatttcaaag
    12481 cttcctcgtc aacagctttt aggacaacat cgggaatgct gtgcattaag aggaaatggc
    12541 tggggaaaga aacatgctac tgtggattat gtttttcact attctcctta tagattattc
    12601 caatatcatc agcttgtcat ggatgaaatg gaaaaacgtg gttacagccc agcaccagag
    12661 tggaaaaatc ctatgtatcg tggcaaatca tgcgaagcat tttttgattt acctactgta
    12721 gagcgcaacg tgccgattta tccggaacac gatgaagttt atctagcaga atgtttagca
    12781 aatttggagc aaaaaggaat ttatcttacc taatataact agaaaagcct tctatctcct
    12841 cttacttcat taaaaagctt taaccatctt agatcaagct aaaatgtcgt aagttttagg
    12901 gttatcttca tcctcacgtg caaaactaaa agcgtactct agttttggtt catcgccaac
    12961 ttccctagac ataaatgaac atctcctgac gtggtttaaa agtaccgcat cttcgacgtc
    13021 tgtatacgat gcacagcaca gattgcttct tttgatcttt cttttgattt ttctgttgct
    13081 tcttctacac cttcttcgat tactacatcg atgcttacaa ttgcttctgc ctaatattcc
    13141 catgcatcgt cgatttaagc ctctgtagtc actgtgatcg tttccacatt ttaactcctc
    13201 tgtcgtggct tcagttgatt ttgtgatcag ttcagttgct aagccagagt cagaacaacc
    13261 tgccattgct agcgttaata ttgatagtaa aaatagtttt tttatcataa ggttcccccc
    13321 ctgaaaatag actaaaataa taacgctttc tttttttgtt ttcagacgaa aattaataat
    13381 ttctaaatat tctttaaaaa agcttataac tattatgcct tttttaatat ataacacagt
    13441 tatttttaaa aataagaatg ctaaatcact ctacaataac ggcgaaagca caaaaaataa
    13501 aaaaattatt acgttattaa aaaaacagat gaaagatcag gttttattag atctcattct
    13561 ttcatcactt gaagggattt aatgaactac tttttcctct ttgtcgtttt tatatattcg
    13621 tattccttca aacagtagga tagcctcttt caatgcttga tatttgaact gtccgttact
    13681 taaaaattta gatgtgccgc cctcttccta gaacatattc ttttttactt tttcattaat
    13741 tgacagacca atcgaacgaa actgtaaatt tcgataggat aatgattggt caaactcatt
    13801 cattttcaag acatctcgat tcatattcaa gataggaatc tttttatcaa catcacttta
    13861 ttcgtatttt cctctaccat aaaagcttca cgctccacaa aattgtttta tacactacta
    13921 ttcagcccat tcaacgacca atg
//
LOCUS       DACDXO010000069        13429 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E23605_2017
            SAMN12639989-rid9881463.denovo.070, whole genome shotgun sequence.
ACCESSION   DACDXO010000069 DACDXO010000000
VERSION     DACDXO010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639989
            Sequence Read Archive: SRR10023389
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741914.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:42:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,972
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 33 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 17 of 73
            Pseudo Genes (multiple problems)  :: 10 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13429
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639989-rid9881463.denovo.070"
                     /isolate="E23605_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..914)
                     /locus_tag="IWM35_002279"
     CDS             complement(<1..914)
                     /locus_tag="IWM35_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HAP9920113.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIY"
     gene            1342..1905
                     /gene="efp"
                     /locus_tag="IWM35_002280"
     CDS             1342..1905
                     /gene="efp"
                     /locus_tag="IWM35_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HAP9920114.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
     gene            2391..3650
                     /gene="lysA"
                     /locus_tag="IWM35_002281"
     CDS             2391..3650
                     /gene="lysA"
                     /locus_tag="IWM35_002281"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HAP9920115.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            3637..4437
                     /gene="dapF"
                     /locus_tag="IWM35_002282"
     CDS             3637..4437
                     /gene="dapF"
                     /locus_tag="IWM35_002282"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HAP9920116.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDTDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            4440..5321
                     /locus_tag="IWM35_002283"
     CDS             4440..5321
                     /locus_tag="IWM35_002283"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HAP9920117.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            5318..6034
                     /locus_tag="IWM35_002284"
     CDS             5318..6034
                     /locus_tag="IWM35_002284"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HAP9920118.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            6040..6735
                     /gene="dapD"
                     /locus_tag="IWM35_002285"
     CDS             6040..6735
                     /gene="dapD"
                     /locus_tag="IWM35_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HAP9920119.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            6751..7752
                     /locus_tag="IWM35_002286"
     CDS             6751..7752
                     /locus_tag="IWM35_002286"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HAP9920120.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            7749..9110
                     /locus_tag="IWM35_002287"
     CDS             7749..9110
                     /locus_tag="IWM35_002287"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HAP9920121.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            9070..10221
                     /locus_tag="IWM35_002288"
     CDS             9070..10221
                     /locus_tag="IWM35_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HAP9920122.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSGAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            10196..11365
                     /locus_tag="IWM35_002289"
     CDS             10196..11365
                     /locus_tag="IWM35_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAP9920123.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            11540..12919
                     /locus_tag="IWM35_002290"
     CDS             11540..12919
                     /locus_tag="IWM35_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAP9920124.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
     gene            13200..13429
                     /locus_tag="IWM35_002291"
                     /pseudo
     CDS             13200..13429
                     /locus_tag="IWM35_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         4482 a         2340 c         2926 g         3681 t
ORIGIN      
        1 aagtaaatgt tttttgataa atagcggtct gctgcgtctg gaaaaatcgt aacgacacga
       61 tgaccaggtg gtaaacgacg aatttctctt aatgctgctg caaaagccgc accactcgaa
      121 cttccaacta atagtccttg ttctcgtgct aactgcctag tatagttaaa cccttctaca
      181 tcagagattg tctcgatctg attgattgaa aggggagaaa gaaacggcgg aatgaattct
      241 acaccgatac cctcaatttc gtgaggggct ggatcaccac catttaacac agagccctct
      301 ggttctacac cgattattcg aatgtcggga tatttttctt tcaaaaaggt agaagttcca
      361 gcaaaggttc caccactccc aattcctgct acaaaactat ggatattttc tttgatttca
      421 tgaaagattt ctggccctaa cgtatgatag taagcagcag gattatcttt attttcaaat
      481 tgaagaggca gatagctgtt tgatatctcc tctgcaagtt ttttactttt gctgatggca
      541 ccgatgattc cttgctcact aggagaatgg atgatctttg ctcccaatgc tttcatcaat
      601 tgttgttttt ctaagctaaa cttctctgga acaacaaaaa ccgttcttaa tttgtatgtg
      661 agtgcggcca gagcgatccc aattcctgta tttccggcag ttggttcgat gatcgtcgtt
      721 ttttcattga tttttccttg ggcaaatcct tctttgatca aataactgcc caatctatct
      781 ttgatacttc ctccaggatt taaatattct agttttgcat aaatagcaga acctttaggc
      841 atagaatagt gctgattgtc aaagcgataa agcggtgtgg aaccaatagc ttctgtaatt
      901 gtcgagataa tcatgtgggc ctcctaaaaa aagataataa aaaaaggagc tgactattaa
      961 atcagctcct tgaaagacat agagaaaaat tgagtagata aaaagataag ttacctggta
     1021 ctcattctat gaagggcaat taatagacat gacaaatagt atgctgcttt tatgcaccat
     1081 acaacagcaa cgattcaatt gtttttggtt gctgactgtc atgtgtatcc ctccaagtaa
     1141 aatttttatt aacgatagca tgaaaaaaaa taaaagtcaa aggcttgaaa gaaaaaaata
     1201 tttcgatagc atcgtttacc cgtgtttatt tttctgacaa ataggcatga gaccttaaaa
     1261 gatagagaat actctagttt tctggccggt tgtctgatac aataaagttg attatgataa
     1321 agaaaagaag gagcaaaaat aatgatctca gcaagtgatt taagagccgg tatgactttc
     1381 gtacaagacg gcaaactgat taaagttctg gatgcaagcc accataaacc tggtaaagga
     1441 aatacagtta tgcgtatgaa actgaaagat gtccgttcag gtgcaacata cgacacaacg
     1501 ttcagaccag aagaaaaatt tgaaaaagca catatcgaca caaaacctgt acaatatttg
     1561 tacacgatgg atgatacagc tttcttcatg gatttggaaa catatgagca atatgaaatt
     1621 cctgtagaaa cagtagaaga agaaatgaaa tatatcctag aaaacatgga agtaaaaatc
     1681 caatttttcg gtagcgaagt catcggcgtt caattaccaa caactgtcgt attacgtgtt
     1741 gtggaaacac aaccttccat caaaggtgca acagtcactg gttcaggtaa gccagcgaca
     1801 atggaaactg gcttagtagt caatgttcct gacttcatcg aaacagatga attgttggaa
     1861 atcaatacag cagaaggttc ttatgtaaaa cgtgcgtcaa aataattcgc taacggattg
     1921 ataagaattt gaaaacagag cttgacctct tgaaaggcag atcatcggat ctgcctttca
     1981 agaggttttt tgtggaatca gcaaattgaa aaataaaaaa gtatttgatt ttatttatat
     2041 tttgaaagat gaatggcaaa agctagagaa agaagaaact gcacttattt atgaagaaat
     2101 tgaaatgatt cataaaattg aaaatatccg aactattccg aactatattg aagcgaaaat
     2161 attttttcat cactaaatag tgttgaattt atgtttttat gtataaatag cttctaatag
     2221 ttcgggtatt ttgtttttat aaccattttt attcttattt ttaaaaaaat atttcgaaat
     2281 atactagaca attcagaaaa ttttgtttaa tattattcca ataaaattca aatctttatc
     2341 aaatgaaatt ccaataaaaa cggaaaaaaa gtgaaaagag gaatgaaaaa atgacagaat
     2401 tgataattgg cggagtcaca gcaagcacat tggcagaaaa gtataagaca cctctgtatg
     2461 tgtatgatga agaaaagatg agacaaacga tgctgtcatt caagcgaggc tttgtatcga
     2521 aacgttttga tacaaaagtg ctttacgcct ctaaagcatt ccaaacgatt gagatgctgc
     2581 atttagttcg tgaatatgga ttcgggctag atgttgtcag tggtggggaa atctataccg
     2641 ctttgcaagc tgattttcct agagatacta tctacttcca tggtaacaac aagacgccag
     2701 aggaactgat ctatgcactt gaaaatgatt tgttgcatat tgttgccgat aatttgatgg
     2761 aagttgaatt attagcagaa ttaagccaac gctatttaaa aaaaatctgt atcatgcttc
     2821 ggttgaatgt cggagtagaa gcacatactc atgaatatat cgtcactgcg catattgatt
     2881 caaaattcgg catgagctat gaaagtaaag agtgccaaca agcccttcag ttgataaaag
     2941 aaagcagcta tctagagtta gaaggatttc atgctcatat cgggtcacaa atttttgatg
     3001 ttactgcttg gttagctgaa atcgacaaat tgacgaatta cttaaccgat tttgaccaac
     3061 ctctttcctt gaatcttggc ggagggtttg ggattcgtta tacacaaaat gatcagccga
     3121 tcccagtcga agaggcacta aaaaaactga ttgaatatac ggaagaagct ttggaaaaac
     3181 gaaaactgac cattcgtcaa ttactgatcg aaccgggtag aagtattgtt ggagaagctg
     3241 gtacaacact atacacgatt ggtttcatca aaaagacacc acataaaaaa tattactttg
     3301 tagatggcgg gatgacagat aatatccgtc cagctctgta tcaggcagaa tacgattgtg
     3361 atttagcagc aaaactgtcg gaagaaaaaa cagagaaagt gacgattgca ggaaaaatgt
     3421 gtgaatcagg cgatgtggta attaaagaag cttatttgcc gaaagcagaa tcgggtgatc
     3481 ttttagctgt ttattccacg ggagcttatg gatactcgat gagcagcaac tacaatcgag
     3541 cgacacgacc agcagttgtt tttgtcaata atggccaatc aagactgatt gttcgcagac
     3601 aagcattttc tgatctacta aggggggaag tttcctatga tccttcataa atataatggc
     3661 tgcggaaatg attttatctt attagattac gcagaagaca cagattatag tcaattagcc
     3721 gcttctttat gcacaaaaga aaaatatgat acagacggat tgatcgccgt aaaaatcgat
     3781 ccgttggaaa tgatctatta caacaaagat ggcagccgcg cgccgatgtg cggaaatggc
     3841 attcgctgtt ttgctcgata cgtaaatgat cagaacatga tgaatgaaca ggaattcaac
     3901 gtagagacat tagcaggcac aagaaaaatt acggtgttgg atccctcacc tttttattgc
     3961 agcgtagata tgggagtgcc agatgattct ccggaaaaaa tcaaagctaa tcaaacttat
     4021 ccaataaaaa accaggaaat agagatcaat ggtgaaaaag taacgatcac tagtttattt
     4081 ttaggaacga ttcatacagt tgtttttgtt gaaaatgcgc gtgaggaagt ctggagaaaa
     4141 agaggtgaac tgatctgtca tcatcctttg ttcaaagaaa aaaccaatgt caactttgta
     4201 gaagtagtca atgaacaaga attgatcgtc cgtacctatg aaagaggagt aggttggaca
     4261 cttgcttgcg gtacaggttg ctgtgcctct tatgtggttg ctaaggaaac tgggaaaatt
     4321 tcagcagatc aggtagttgt tcacttggaa caaggagact tgatgatctc tggtgaggat
     4381 gcgatacaaa tggctggccc agcagttcat gaatggacaa gaatattgga ggaatgaaga
     4441 tgataaaagg ttcgatcgtg gcgctgatca cgccgatgaa tgaagaggga tcagtagatt
     4501 atgctggttt ggaaaaattg atccaattcc atttagatga acaaacagat ggattattag
     4561 ttttaggtac aacaggagaa tcttctacgc tcacacaatc tgaggaggag cagatccttc
     4621 aactgacagt aaagaaagtg gctggccgtg tgccggtgat tgcaggagct ggaacgaata
     4681 atacgaaaga aacgattgaa aaagcgaagc attttgcttc tttgggggca gacgcacttt
     4741 tggtcatcac cccttactac aacaaaacaa gtgatgcagg actagctgct cattttacag
     4801 caattgcaga agcctcaccg attccgctta ttttgtataa tgttccttct agaacaggca
     4861 tgtcgattcc aatccatgta ttagtaaatt tagcagaaca tcctaatatc attggtttga
     4921 aagaagcctc aggagatatg gcttatgtga tggatgccgc tcggttgatt ggagaagaat
     4981 tcttccttta cagtggaaat gatgatttga tcctaccagt aatgagtgta ggaggaagcg
     5041 gtgtaataag tgtatgggcg aatatccaac ctaaaatagt ccatgaatta gtaaaagata
     5101 cgcaggacgg aagatggcag caagcaaaag aaaagcaatt aaatgcactt gaactgatcc
     5161 atgccttgtt cagtgagacc aatcctattc ctgtcaaagc agccatgtca ttattagact
     5221 taccttccgg acctctacgc ctgcctttag tttctctttc aaaagagaaa aagaaacaat
     5281 tagctcagct tttgttaaaa gaaaggacgg ttgaaaaatg aaaatcggac tgattggcag
     5341 tggacagatg ggaaatagag tcgaagaagt tgcacaagaa agaggagata cggtccttct
     5401 tttacggacg gcacctaaag aagcaactac gcttgctttt acggaactgc cagaactgct
     5461 tattgatttt tctcatccag ataatttaga gaaaatcctt tcgtatagct tatcatatca
     5521 acttccgtta gtcattggca cgacaggtta tacacaatca cagttccaag aaataaaaga
     5581 acatgccaaa catgtacctg tgatgtatag tgctaatttt tcgttcggca tcatggtgat
     5641 gaatcaacta atcaagcaag cagctgcaat gttgcaaggc tggcagatcg aactgattga
     5701 aaaacatcat agtcgtaaaa aagatgcacc atctggtact gcaaatatgc tgcttcaaac
     5761 aatccaagaa gtgcaaaagc tgcagcccgt atacaactgg caagggaaaa aaagagaaga
     5821 aaaccaaatt ggtgtacaca gtattcgtgc aggttcttta cctggtgaac atgatgtatt
     5881 gtttgctgct actgatgaaa tatttacgat caaacatgaa tctttttcta atcgaatctt
     5941 tgctgcaggc gcagtagaag cagcggactg gttgaaagat cagtcaccag gttattacaa
     6001 attagaagat ttattaatgg acaaaggggt gtaaaaaacg tggaagctca agaaatcatc
     6061 cgctacattc aaacggcatc taaaaagacg cctgtgaaag tctatctgaa tgtaaccgag
     6121 ccaatcgaat ttaaagaaag taaagttttt ggtgaaggaa gcagcttcgt tgtttttggt
     6181 gactatgaga cgattgctcc ggttttagaa gctgaatcag aaaaaatcat tgaaatcgaa
     6241 atcgaaactg ctgcaagata cagcgcagtt cctttgctag atattaaaaa aattaatgct
     6301 cgtatcgaac caggtgctat tatcagagat caagtgtcaa tcggcaacaa tgccgtgatc
     6361 atgatgggag cgattatcaa tattggtgca gtaatcggtg aaaacacgat gattgatatg
     6421 ggtgctgtct taggtggacg agcgacagtg ggcaaaaact gccatattgg tgcaggagct
     6481 gtcttagcag gtgtgatcga accagcatcg gccaaaccgg ttatcgtaga agacggcgta
     6541 ctggtaggtg cgaatgcagt gattgttgaa ggtgtccata tcggaaaaga tgcagtcgtg
     6601 gcagcaggag cagtggtttt agaagatgtg gcagcagaaa ctgttgtcgg tggaatacct
     6661 gcacgtgtat taaaaataag tgatgatcag acaaaagaaa atacagcatt gattgctgcg
     6721 ttacgcgaat tatagaggag atggagaaaa atgcgcgaaa tagatacctg tattgtgggg
     6781 gcaaccggat tagtcggcca aacgatgctc aaagtgttag aagaatatga ttttccagta
     6841 ggacgattga aattattagc ttcaaaacgt tcagccggta aagtttgtgt attcaagaaa
     6901 aagaaacaca ctgtacagga attgacagaa acttcatttg aaggatatga tttagcgcta
     6961 ttttctgcag gtgcttctgt atccaaagag tttgcgccaa tcgcacgaag tcagggacta
     7021 tttgtcatag ataattcctc agcatggcgg gaagattcgg atattccatt aatcgttcca
     7081 gaggtcaatc tggaagacta tacattgaat cggctgattg caaatccaaa ttgttcaacc
     7141 attcaagcgg tacttccgct taaagcttta caagaagcct ttggtatcca gcgagtcaat
     7201 tattcgacct atcaagcagt ttctggttcc gggcaaaaag gcattgaaga tcttcatcgt
     7261 acgcaaaatg acgaggaacc agttttgtat ccttataata ttagccaaac ggtgattccg
     7321 gaaatcgatc agcctttgga aaatgggtat acaaaagaag aacagaaaat gataaatgag
     7381 acccgtaaga ttctgcatca gcctgctttg ccagtatcag caacctgcgt acgtgtgcca
     7441 atcgaaaatg gacatggagt ctctgtggca gttcaactgc aaaaagagtt tacgacggaa
     7501 gaaattagag agtgtctcag ccagtttcca gggattcaat tagaagatga tctagcagca
     7561 cataaatatc cgacgagcat tcttgctaga ggaacagata tggtttatgt tgggaggatc
     7621 agaaaagaca cttctttaga gaacggattg ttgttctata cgactgccga taacatacgc
     7681 aaaggagccg ctgcgaatgc cgtacagatt gctgcagctt taatgcagaa aatagcggag
     7741 gtggcaagat gagtaatacg ctgaaagtag cgaaattcgg cggaacatcg atgtctcatg
     7801 aagagcaatt caaaaaagtc cgtacaattg ttctttcaga tcccaatcgc caagtgatcg
     7861 ttgtttctgc tttaggcaaa cgtacgtcag atgatgacaa agtaacagac ttactttatt
     7921 tgatttatgc ttaccttcag catcatgttg attggacacc cctttggaaa aggatttgtg
     7981 atcgtttcat tcaagtaaga gatgttcttg ccctgcagtt acctattgaa gatgaactag
     8041 cacgcataca gaaacaactg gaagacgaaa agataactga agattattta gtaagtagag
     8101 gtgaatatct gactgctcaa ctgatggctg agtatcttgg ttaccagttt attgacgcaa
     8161 aagacctgat tttctttgac tatgatggga aaatcgatca ggaaaaaaca gcacaggaaa
     8221 tcacaaaagc atttaaaaga tatccgaaaa tggttgttcc agggttttac ggggcgaacc
     8281 ctgcaggaaa agaaaaatta ttgggccgtg ggggaagcga cattaccggc gcgatactgg
     8341 cgaagatgtt gcaagcggaa agttatgaga attggacgga tgtttcagga atcatgatgg
     8401 ctgatccgcg aatcatcgat catcctaaaa tcatcgagga aatctctttt agagaactga
     8461 gagagatggc ttatatgggg gctaacgttc ttcatgaagc agctgttttc ccagttcaag
     8521 aggctgatat ccccatccaa ataaaaaata ccaatgatcc tttcgctcaa gggacaaaaa
     8581 tatcgaatca cgaaatagaa aaggaaaatg gactgacagg aatagctgga cgcaaggatt
     8641 ttctttccat cactttattc aaacgtcata tgtccgatga aatcgggttt atctggaaag
     8701 caatgagcat ttttgctaaa catgagatat caatcgaaca tattccttca gggatcgaca
     8761 atatcggagt gatcgtctca gcagaagcga ttgccgataa gttatttctc atcacgaaag
     8821 aattaaaaga aacacttggc gtggaagaaa tcgtcgtgat cgaagactta gctttgatct
     8881 ctgtcgttgg gggtccgcat aaagaattga ttggattatc tgggaaagtg ttatcgatat
     8941 tgaacaaatt agaaattcgc acatcgatct tatcacaagg agcacaggag ttgaatctga
     9001 tcatcggtgt accgaataat caatatgaaa cagtagtgaa gggtatctat gaagggatgg
     9061 tgaatacgga tgctgaaaga atatcgaatg gcactccatc aaattcctga aatcgggttt
     9121 catgaattca agacaaaaaa atatttatat gaacaagtaa aaaacagcgg ggccgttatc
     9181 catgaaatcg gcgaaacagg actactcctt tattttgata atcagcaaga agaaacgatt
     9241 gcttttcgaa cagatatcga tgcactgcca gtcaaagaag aaacgaatct ctcttttgct
     9301 tctaagcatg aaggttatat gcatgcatgt ggtcatgacg gacatatggc gatgctttta
     9361 gggttgactg actttataac taaacacatt gaggatctta tttataatgt agtactgatc
     9421 tatcagccat cagaagaaat ttccggtggc gcacaaagcg ttattcagtc aggattgttg
     9481 gataaatatg aagtacaagc aatatttggc tttcatctat ggcctggact agctaaggga
     9541 gaagttttct ctcgaccagg acctttgatg gcacaaagca gtgaaacaga cattatcatt
     9601 aaaggccagt ctgcacatat tgcttctagt gaaaaaggaa tcgatagtct ggaggcagct
     9661 gtacgatttt tgaagaatgt ttatgatttt gaccggcaat taccggcaga gtggaatcat
     9721 ctaatgaaat ttggccaaat ccaaggtgga acgatccgca atgttttagc tggagaagtt
     9781 attgtatccg gaagtatccg ttcttacagt tcagatatgc aaaattattt gaagagggga
     9841 ttagaccatt tagcagaaca gtttcactct acagctacta tttcttttcg ctataatgac
     9901 ggctatccag ctgtaatcaa ccatagaggc ttatatgcag cactgttaga ttcaaacaaa
     9961 ctacatgaat tagatgctcc agttcttcaa gccgaagatt ttggcatata tacgcaacaa
    10021 tatccatgtg tatttttctt tttaggtgtc ggtgataccc ctgctttaca tgatccgacg
    10081 ttcgattttg atatggatgt tttagaaaat ggtgtagaat ggtacaaaac gattcttttt
    10141 acagaaaatc ttcttgtaaa aatcgatagc agttcaaaag aggagcgagt aaataatgat
    10201 gaatttatcc aatcgattta gtcctaggct ttctagaata gaagtatcaa aaatacgaca
    10261 attcgatcag caaatatcgt cgataccaga tgtgatcaaa ctcaccctgg gagaaccgga
    10321 ttttccaact cctgaacatg tcaagcaagc aggaatagca gcgattgaag aagacttttc
    10381 tcactatacc ggaatgagag gattagagga actgcgggaa gcggcttgta tatttcagca
    10441 acagcgttat ggattaactt atgatccgca aacggaagtt ttgacgacag ttggtgcaac
    10501 ggaagcgatt gcctctgcgt tgttatctgt tttggaagaa ggagacaagg ttcttattcc
    10561 agcgcctgct tattcaggat atcagccatt agtggaacta gctggtgctg aattgatccc
    10621 gatagatact tctgacacag ggtttgtctg tcagccggaa caatttgaac gagcatttga
    10681 acaatatgga tcagctgtca aagcagtcat tttaaactat cctaacaatc ctactgggac
    10741 gacattgtct gcaaaacaat tgaaagcaat cgctgaggtt ttaaaaaaat atccggtatt
    10801 tgttataagt gatgaagtat atgctgaatt gacttatagt ggaacgcata tgtccatagc
    10861 tacttatctt ccagaacaaa caatcgtgat aagcgggtta tccaagtcac atgcgatgac
    10921 tggttggcga gtgggcttta tttttgctca aaaagccctt attgacgaac taattaaagt
    10981 ccatcaatat ctggtgactg ccgctacaac gatgtcgcaa aaagctgcgg ctgaagcgct
    11041 aataaatggg gtgaaagata gcgagaatat gaaagagcaa tatcaaacac gaagagacta
    11101 tctggtccag caattggcgc ctttaggatt tgaagtgacc cagccaaacg gtgcatttta
    11161 cttgttttgc aaactcccag ctgccattca aactgactct tggcagtttt gtgtagaatt
    11221 agcggaacag gcaaaagttg cgtgtatccc aggaattgca tttggcccag aaggagaagg
    11281 ttatatccgg atttcttatg ctagcagcat ggaaaaatta catgaggcct gcaaacggat
    11341 caaagacttt ttaacagaac agtgattcat agattttatc aaaaaaacaa tcgttaattt
    11401 tgcggtatat tctgcaagga gacgattgtt ttttaaattt agttcaaatg aacgcgtttc
    11461 ttttgattca taactaatgt aatgttaagt ttaaattgtt tagaatgatt ttaaaagaat
    11521 attcaaggag gtttcatcaa tggaaaaaat caaagttgtc attgtaggtg catctcatgg
    11581 agggcatcaa tcgatcttgg aactattgac acgatacggt gaaaatgtag atatcacgct
    11641 atttgaagca ggagactata tttccttcat gtcatgtggt atggagctgt atttagaaga
    11701 caaggtaaca gatgtaaatg atgtacgtaa tttcagacca gaaaactttc cgcaatccaa
    11761 tgttcatatt ttaaataatc acgaagtaaa aacgatcgat gccgataaaa aaacagtaac
    11821 ggttactagc gcatcagata acgaaacaaa agaatatcct tatgacaaat tgattttgag
    11881 ttctggtgtc aaaccaaaca atctgccagt accaggagca gatctagaaa atgtctactt
    11941 gatgcgtgga tacaattggg caaccaaaat caaagaaaaa ttaaatgatc cggctattaa
    12001 aaaaatcgct gtcgtaggtg cgggttacat tgggatcgaa gcagcagaag caagccgaaa
    12061 agctgggaaa gaagtcgtat tgcttgatgt gatcgataga cctttaggca cttatttaga
    12121 tgctgagatg acagatattt tagaacaaca cctaaatgaa aacggtattg aagttttgac
    12181 aaatgcaaaa atcgaagctt ttactggtaa cggaaaagtt gaggcaatca aaacaagtga
    12241 aaaagaaatc ccagcagatc tagtcattca agctgccggt gtgaaggcaa atacagaatg
    12301 gttaaaaggg atcgtcgact tagatgaacg tggctggatc caaacaaatg aatacttgca
    12361 aacgaatctt ccagatgtgt atgcagtagg agatgctaca cttgcttatt ctatcccagc
    12421 acgcaaaaaa atgccgatcg ctttagcgac tgttgcacgt agagaagcac gttatgtagt
    12481 aaaacatctt tttgaagaag taccaaacaa accgttcggc ggagtcgtag gttcctctgc
    12541 attaagtgtc tttgactatc attttgcagc aagcggattg aatagcttta cagcagcaaa
    12601 agcaggagta gatgtacgag tgtcttatta tgaagatacg ttacgaccaa aatatgtacc
    12661 agaagaacaa ggaaatccaa aagtgtccat ccaattattc tcggatccaa tgacccatca
    12721 attgctagga ggagcagtat tatctaccta tgatgttaca ggtcaaggaa atgtattggc
    12781 actagcgatc caaaacaaac tgactttaga agaactagca gaagctgact tcttcttcca
    12841 gccaggcttt gaccgacaat ggagcatgtt gaatctagcc gctcaacaag cgttaggaga
    12901 agaaccattt gtagaataag aaaacgaaat atttgaaaag aggttgtgaa aaagctgcct
    12961 tgcatcaagt aataagaaca gccaagtaaa aacagcttct tatgttttta acttggctgg
    13021 acttattacc gcagatgcaa gcttttgaac accgtttatt ttcaaaaaag ggactgtgac
    13081 acgatttgtg taccgacccc caaaagttag accaaaaaat ctaacttttg gaggtcggta
    13141 ttttttatgg ttaaatatac ttttgaactt aaattaaaga tcgttcatga ttatttggat
    13201 ggcaaaggcg attggaccgt aaaaatccag atcaagagat tgaagcggaa atgcttaaaa
    13261 ttcgggagaa gcacaaggat tatggctgtt tacggatgac caatgaacta agaaaccgcg
    13321 gtttctctat caataaaaag aaagttcagc gcttgatcaa gaagctagga ataaaagtaa
    13381 cggcatacac gcgtaaatca cgtcgctaca attcttatcg aggaaaagt
//
LOCUS       DACDXO010000070        13404 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E23605_2017
            SAMN12639989-rid9881463.denovo.071, whole genome shotgun sequence.
ACCESSION   DACDXO010000070 DACDXO010000000
VERSION     DACDXO010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639989
            Sequence Read Archive: SRR10023389
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741914.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:42:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,972
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 33 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 17 of 73
            Pseudo Genes (multiple problems)  :: 10 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13404
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639989-rid9881463.denovo.071"
                     /isolate="E23605_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            79..1125
                     /locus_tag="IWM35_002292"
     CDS             79..1125
                     /locus_tag="IWM35_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HAP9920125.1"
                     /translation="MTKPNHIESSKKEAPKTEGQTKTFSTPQQIESTESFQSQPILSG
                     NEEIHFDKNESVENFIMKIGESARKIGQKYDLYASVMIAQAILESASGQSQLAQAPNY
                     NLFGIKGTYNGNFVIMVTNEDLGNGTLYTTQSKFRVYENYEESFEDYAKLLTKGISGN
                     KDFYAGALKANSKTYREATKFLTGRYATDTQYYLKLNELIKTYDLTNYDKEVKGPQMS
                     KTGYQVPIHNYSITSLFGNRGNEFHRGLDMAATQGEPIHAVKAGKVVKAEFHYSWGNY
                     VVIQHEDGSTALYAHQQQYLVKTGDNVLQGQIIGYVGSTGNSTGPHLHLEICKDSSLS
                     QSMLVDPKTLLFGD"
     gene            1474..2106
                     /locus_tag="IWM35_002293"
     CDS             1474..2106
                     /locus_tag="IWM35_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HAP9920126.1"
                     /translation="MKERYGHLTVVEEITHNGYKKYRCLCDCGNETIVYRSNLLSGRT
                     KSCGCGNYKNRHKYKNLTNKTFGKLVAIKPTKLRSEGTVVWECQCLCGRKKLVSAKRL
                     LKGDVKSCGCLKSKKHHIENKKFGLLTAKYPLDNNCSWKTKWVCHCECGSMCEISYSN
                     LFFGHTQSCGCLKRQETRTCVEGTVIECLTSKLPKNNTSGVKGVYRSKEK"
     gene            2694..4133
                     /locus_tag="IWM35_002294"
     CDS             2694..4133
                     /locus_tag="IWM35_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP9920127.1"
                     /translation="MYSYQEDAKVQKNRWWILIAISMFTFMSTLDGSIVNIALPTISD
                     DMGIPMNQAEWVVSVYLMIVCSCLLLFGKIGDSRGKIKVFRIGTLIFVLGSLLCGFNH
                     SLGILLFARVVQAIGASMTMATNAGIITEVFPIHERGRALGAIGAFVSLGSIAGPGLG
                     GLILSQFSWAYIFWINVPVGIVTMLIAEKFLPKDITMSGHTIDGGGFAAIAATIISFF
                     GAIFLGQESGFFHPVPLVLFVISVIAFVSFLKVEKKAASPLISFGIFKNKIFTMSLIT
                     ALLIFSSNFFVNVVIPFYLQNARGLSPSYAGLLMMVFPLMMVVGAPLSGYLTDRVGPE
                     ILVLVGLGLLSVTQVMYMFLSVDSPLWLYIVATAIMGAGNALFQSPNNTMVMSSVTIE
                     NLGLAGSMNSFARNLGMVSGISLSTTVLYQAMSAKMGQQVTNYIANRPDVFLYGMKIT
                     FLGSFVLCFVAFFLTIWRIKKQGGIQKNV"
     gene            4428..4646
                     /locus_tag="IWM35_002295"
                     /pseudo
     CDS             4428..4646
                     /locus_tag="IWM35_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174271.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            4895..5110
                     /locus_tag="IWM35_002296"
     CDS             4895..5110
                     /locus_tag="IWM35_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9920128.1"
                     /translation="MNAGIADMNLIKKTLNDFTSNSISKGTGINLSTIKKLKSGERSV
                     EKLNLLDAIKITEFAMKNGKAEIEIWR"
     gene            complement(5327..5583)
                     /locus_tag="IWM35_002297"
                     /pseudo
     CDS             complement(5327..5583)
                     /locus_tag="IWM35_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(5770..6681)
                     /locus_tag="IWM35_002298"
     CDS             complement(5770..6681)
                     /locus_tag="IWM35_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP9920129.1"
                     /translation="MKIKIERNQKEKSPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GVYKNFTAIDQHTTHEKVVIKEKVVNTSGVESFTEDFAKEYFSWKNNKEAIEKRMSNL
                     EQYLTENGLALIQDMVRADIPTSSEVQSVKILDVEKHSEEFVVSFLVEQHITEGKKTQ
                     SISSAYRVTIFEDENRNHLVTSLPTMIGKPDKAKYEAKQVENDSEIDAKTTEEITDFL
                     ETFFKLYPSASEKELEYYVENGAMRPINIDLKFSELENPVYLQNGKNFLVSTKIKYLD
                     NVSKTTSYFQYKLVLQKNGNWKIVDVK"
     gene            complement(6700..7704)
                     /locus_tag="IWM35_002299"
     CDS             complement(6700..7704)
                     /locus_tag="IWM35_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase P60"
                     /protein_id="HAP9920130.1"
                     /translation="MNLKKVGVISLTFLTISFMGILMCVGLLFGEDSEGNGSSGTSPG
                     GTSVSEEVLKHRAMVEKYAKESDITEYIPILLAIIQVESGGTMEDVMQSSESAGLPPN
                     SLSTEASIKQGCLYFASLVKRSKELGCDQDSIIQAYNYGGGYLDHVGKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENGGWRYNYGNMFYCLLVKQYLTTTQFDDKIVQEIF
                     DEAFKYEGTAYVFGGSSPETGFDCSGLTQWCYAKVDLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLVFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTENYWRQHLIGAGRYK
                     "
     gene            complement(7701..9824)
                     /locus_tag="IWM35_002300"
     CDS             complement(7701..9824)
                     /locus_tag="IWM35_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAP9920131.1"
                     /translation="MKKKLFRIVGIVFISILTLIVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAESIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRAVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALTLGTEIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEDRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKKAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRALAFGGGAAVGNKLAKEQSKSKFKPSGSSLRKNSRLPNDHEILSDLTKEN
                     PVSNNKKQSRMNLAGRKIGKVLDTQALVKDKTKQVKDQVRNTPTNLKYNLHKGIEKSK
                     KAPEEFKRGLFQEKADRVDLREKQRQRRNAKMDEKRKILSEATNRQGKRRANIPIDKA
                     AQPQKDMMLKKEAPKRIVRANLNPEIKRKLTSQEIISKGKSQSASKKNSFQQVKQRST
                     LPKQTNQKVQKRMNHRPKSRSGEKK"
     gene            complement(9829..12276)
                     /locus_tag="IWM35_002301"
     CDS             complement(9829..12276)
                     /locus_tag="IWM35_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAP9920132.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELIPYNYSFLSPEQKYQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLTEVAKQRIDLQTEALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKNLKKSFFSGFQEFVYGVNHHLMGDFVSLSNEEI
                     RRYTKLEKLMESKLARRFKVRRVTPSDLMYLIEHIYGEKGTPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPSRCLIEEKPRYLCMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKDSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEINILNLTSKPKNQGLLDPYVIMEKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSNQRGLLQVINELRKEGSPVAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDRQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDREDESNQKRLRDLENGQCLMQDLYGRVGVVQVHPVFE
                     ELFHAFDTRPPVQERME"
     gene            complement(12263..12652)
                     /locus_tag="IWM35_002302"
     CDS             complement(12263..12652)
                     /locus_tag="IWM35_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP9920133.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEGAFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPITLVRSVNYVPD"
     gene            complement(12712..13215)
                     /locus_tag="IWM35_002303"
     CDS             complement(12712..13215)
                     /locus_tag="IWM35_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HAP9920134.1"
                     /translation="MEQMRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELEGTPVGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCDDMSDVAYFLIVEAGDFGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFEYPY"
     gene            complement(13228..>13404)
                     /locus_tag="IWM35_002304"
     CDS             complement(13228..>13404)
                     /locus_tag="IWM35_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9920135.1"
                     /translation="QSLVLLAIVVIGLFLGFKREFSKLIGFLVIALIAVGLVFNASGV
                     KDVLLSLFNRIVGA"
BASE COUNT         4099 a         2642 c         2454 g         4209 t
ORIGIN      
        1 tcaagtgaaa gtagtacgat ttcaagtacc gaatcttcaa caacttcaag tagtacaagt
       61 gaaataactt cttcgaaagt gacaaaacca aaccatatag aatcttcaaa aaaggaagcg
      121 ccaaaaacgg aaggacaaac aaaaactttt tcgacaccac agcaaataga atcaacagaa
      181 tctttccaat cacaaccaat actatctggt aatgaagaaa ttcattttga caaaaatgaa
      241 tctgtagaaa attttattat gaaaattggt gagtctgcga gaaaaattgg tcagaaatat
      301 gatttatatg cttctgttat gattgcacaa gcgatcttag aatctgccag tggtcaaagc
      361 cagttagctc aggcaccgaa ttacaattta tttgggataa aaggaactta taatggaaac
      421 tttgtcatta tggtaacaaa cgaagatttg ggtaatggaa cgctttatac gacccaatca
      481 aaatttagag tgtatgaaaa ctatgaggaa agctttgaag attatgccaa attattaact
      541 aagggaatta gtggaaataa agatttttat gcaggagcat taaaagctaa tagtaaaaca
      601 tatcgagaag caactaaatt ccttacagga cggtatgcaa cagatacaca atactatttg
      661 aagctaaatg aattaattaa aacctacgat ttaaccaact atgataaaga agttaaaggt
      721 ccacaaatga gtaagactgg ataccaagtg cctattcata attattcaat aaccagtttg
      781 tttggaaatc gtggaaatga atttcaccga ggacttgata tggcagctac acagggtgag
      841 ccgattcatg cagttaaagc tggtaaggtt gttaaagcag agtttcacta ctcttgggga
      901 aattacgtgg tgatacaaca cgaagatgga tcaaccgcgt tatatgctca ccagcaacaa
      961 tacctagtaa aaactggaga taatgttttg caaggacaaa taattggcta tgtaggaagt
     1021 actgggaata gtactggccc acaccttcat ttggaaattt gcaaagatag tagtctttct
     1081 cagagcatgt tagttgatcc gaaaacctta ttattcggag actaaaaaag gtaatcaatc
     1141 tgacaaagta acaagaaaat ggttggattc ttagaccttt gatcaggata ttgagactgg
     1201 aattgatatc tcgattacga tgggtttgac aaataaaaca agtccattat cgaatatcga
     1261 gtgtattatt gccgtcttta tgctcatatt ttgagcaggt ttcactagat gaaaacaatt
     1321 tatctatctt gaggatttcg tgtacgtatc gttcagtctt gtattgtaaa ttagtcataa
     1381 aatcagacta agaaaaattg gaaatatttt tttctgattt attaaaagtt aattgaaatt
     1441 atatggtact tctttcttgg gagggtaggt gcagtgaagg aaagatatgg tcaccttact
     1501 gttgttgagg agattactca taatggatat aaaaaatatc gttgcctatg tgactgtggg
     1561 aatgaaacga ttgtatatcg atcaaactta ctatcaggta gaaccaaaag ttgtggatgt
     1621 ggaaattata aaaataggca taaatataaa aatttgacaa ataaaacttt tggtaagtta
     1681 gttgcaatca agccaactaa actgcgaagt gaaggaactg ttgtttggga atgtcaatgt
     1741 ctgtgtggga gaaaaaagtt agtatcggca aagcgacttc taaaaggtga tgtaaaaagc
     1801 tgtggatgtt tgaaatcaaa aaagcatcac attgaaaata aaaaattcgg attacttact
     1861 gcaaagtatc ctttggacaa taactgttca tggaaaacaa aatgggtttg tcattgtgag
     1921 tgtggcagta tgtgtgagat aagttatagt aacttatttt ttggacatac tcaaagttgt
     1981 ggatgtttaa aaaggcaaga aacaaggaca tgtgttgaag ggacagtaat agagtgtcta
     2041 acgtcaaaat taccgaaaaa taatacaagt ggtgttaagg gagtttatcg ttctaaagag
     2101 aaatagattg cgtatataac tttaaaaaaa aagaggtact atttaggatc gtatgattct
     2161 attaagagtg cagctcaagc tagaaaagat gctgaaaata aattgtttca acctttgatt
     2221 gacaaatatc gaggtaatca ttaattattg tggataataa ttaacagtga tatataaaca
     2281 tctataatat attattcata ctattgatat ttagtataat cctatggaat tgaatatcat
     2341 agcaatgtat ttgaaaagca aattggaaaa caaataggtg caacaccata aatatgaaaa
     2401 aattgggaaa aagtagaatg gaaatttgct tacgtctcac gagatagctc acactattaa
     2461 tctgaagaac aatgaagtcg acttttagaa attgctagaa gaaagtaaag cgaattatca
     2521 ctagttacta tagagtaagt tcaaataaag catctccaac ttacatggtt acatgaggat
     2581 atcaaatcga aattactcaa gcttcctatg gctagagtcg cgagaagttg ttgggcagta
     2641 gacataggtt atattgttct tagctacacg atcattagaa aggaaatcct attatgtata
     2701 gttatcaaga agatgcaaaa gtccaaaaaa atcgctggtg gattttgatt gctatatcta
     2761 tgtttacctt catgtccaca ttggatggca gtattgtcaa tattgcacta cctaccatct
     2821 cagatgatat gggcatacca atgaatcaag cggaatgggt agtgtccgtt tatctgatga
     2881 ttgtctgttc ttgtttgttg ctgtttggta aaattggcga tagccgtggg aaaatcaaag
     2941 tatttcggat tggtacgctg atctttgtct tgggatcgct gttgtgtggg ttcaatcatt
     3001 cgctaggtat tttattgttc gcacgggtgg tacaagccat tggtgctagt atgactatgg
     3061 ctacaaatgc cggaatcatc acggaagtct ttcccataca tgaacgtggt cgggcattgg
     3121 gagcaatagg tgcgtttgtt tcattaggct cgattgctgg accaggtctt ggcggcttga
     3181 ttttgtccca attttcttgg gcgtacattt tttggatcaa tgtaccggtg ggaatcgtaa
     3241 cgatgttgat tgctgaaaaa ttcttaccaa aagatatcac catgagcggc catacaatcg
     3301 atggaggggg ctttgctgcc attgcggcta cgattatcag cttttttgga gcaatctttt
     3361 tggggcaaga aagtggcttt ttccatccag tcccccttgt actgttcgta atttcagtga
     3421 ttgcctttgt cagcttttta aaagttgaaa agaaagcagc ctctccattg atttctttcg
     3481 gtatttttaa gaacaagatt tttaccatga gcttgattac agccttgttg attttttctt
     3541 ccaacttctt tgtcaatgta gtgattcctt tttatctgca aaatgctcgc gggttgagtc
     3601 ctagctatgc tgggctgttg atgatggtct ttcctttgat gatggtcgtc ggggcgcctt
     3661 taagcggcta cctgaccgac cgggttggcc cggagatttt ggtattagta ggcttgggct
     3721 tattgtcggt aacccaggtc atgtatatgt ttttgtcagt ggactctcct ttgtggctat
     3781 acattgtcgc tactgccatc atgggtgccg gcaatgcctt gttccagtcc cccaataata
     3841 cgatggttat gagctcggtc actatcgaaa acttgggact ggcaggctcg atgaattcct
     3901 ttgcccgaaa tttggggatg gtcagcggga tttcgctttc tacgacggtc ttatatcaag
     3961 cgatgtctgc taagatgggc cagcaagtaa ctaactatat cgcaaatcgg ccggatgtct
     4021 ttttatatgg aatgaagatt acctttttag gcagctttgt tttgtgtttt gttgcctttt
     4081 tcctgactat ctggcgtatc aaaaaacaag gaggtattca gaaaaatgtc tgaaaaaacc
     4141 actgttgaag aatttttgga ggcctgcgta tgggtgagtg ggatttccat ggatgatgga
     4201 agatgaagtg aacttctaaa agccgaaaaa ctcaagtatt atcttcaaaa tactagtgct
     4261 caatgagatg cttgatttga tcacaagaag actgcgaaag ccgctggcaa ccgcaagcta
     4321 ggtatcccac cgatggacta cgttttagag gctactggtt atgaatacgg agccaacgct
     4381 ctcatctgca taagaataca tcacccagat tatgtaaaga ttttcgatac ttctgtcaaa
     4441 aaatcattaa gatagataaa tgatttccgt ctagtcaaat tggttcagaa tgtagacaca
     4501 cagatggtaa gaagtcactc acaattcgag aatggacttg tcctatttgc cgtatacatc
     4561 ataatcgaaa cgtcaacgat agtatcaata ttttgaccaa gggctatgaa tacaagcatt
     4621 gccttagaaa acatcaagaa ccttaggaac aacggggata gcttggttaa taagagatac
     4681 cgctgtttag gcttgcaagt atactctttt atcaagaatt ccgtggcttt agtcatgaga
     4741 ggcttaatac tatgaaaatt ttctttagca tttttgattt aggttccttt tctaattata
     4801 ataagatgac cactacagta acctactgaa atatataaac agccactgta gtaccatttt
     4861 caaagtagtc tatgagagga gaatcttcat tcttatgaac gctggaattg cggatatgaa
     4921 tttaataaaa aaaaccctta atgattttac ttccaattca atatcaaaag gtactggtat
     4981 caatttaagc acaataaaaa agctgaaaag tggtgaaaga tcagtggaaa agctgaatct
     5041 tttagatgct ataaaaataa cggaatttgc tatgaaaaat ggaaaagctg agattgaaat
     5101 atggagataa ttatcgtcta gtgaattact tgccacttag ctgaatttaa cgatccttgg
     5161 tgcttgaagg gggagattct tgggaataga gcatacttgc aagcctatgt ctttactaac
     5221 aacgatatct cttattaacc aagctatccc cgtagttcta aggcttttac ttttatctaa
     5281 gcaataatcg tattcaaagg aaatttttga cccttagaaa gtctcatttt atcagacgtg
     5341 agcttaggca ttgctgaatt ttgttaattc ggttttaggt tgtgctttga gcataatatg
     5401 aaagtattct ttatcacgat ttcattcatc taaggtaatg tgatgtgatt ctgaaattct
     5461 ttcaaaggca gtttttgcat aagctgacat tacatcatca atgacatgtt tacaatgttt
     5521 cactactaaa ataagggggt aaccaagaag aaaaactgaa tgattatttg tatctaattc
     5581 catggtatat caaccatttc ctatatagac tgattatgac atggattaaa ataaaaaggc
     5641 actatgtctt aattgtcggt tttcgagtta ccgaatggca ttcatcaccc acctatagag
     5701 gatgggcgac ttctgcctga aataagttac actacttata acttctaata aaattagatt
     5761 gagtcattat tatttaacat caacgatttt ccagtttcca tttttttgta atactaattt
     5821 gtactgaaag tagctagtgg tttttgatac attatcaagg tattttatct ttgtactcac
     5881 tagaaaattt tttccgtttt gtagataaac tggattctct aactccgaaa actttaagtc
     5941 aatattgatt ggtcgcatcg caccattttc aacataatac tccaattctt tttcagaagc
     6001 tgatggataa agtttaaaaa atgtttctaa aaaatcggtg atttcttccg ttgttttggc
     6061 atcaatttct gaatcgtttt ctacttgttt tgcttcatat tttgccttgt ctggctttcc
     6121 aatcattgtt ggcaaactag ttactaaatg attcctgttt tcatcttcaa agattgtcac
     6181 tcgatatgca gaagaaattg attgcgtctt ttttccctct gtaatatgtt gctccactaa
     6241 aaatgaaacg acaaattctt ccgagtgttt ttcaacatct agaattttta cagattgaac
     6301 ttcggaactc gtgggaatat ctgctcgaac catatcttgg attaaagcga gaccgttttc
     6361 tgtaaggtac tgttcgagat tactcattct cttttcaatt gcttctttgt tatttttcca
     6421 tgaaaaatat tctttcgcaa aatcctctgt aaaactttca acgccagaag tattcactac
     6481 cttctctttt ataaccactt tttcatgagt tgtatgttga tcaatggcgg tgaaattttt
     6541 ataaacgcca aaagcaagac tacaagacaa taaaagccaa agggcaagca ccatttttcg
     6601 atgcttaccc acggatacaa ctcgttcttt tttctttttt gggctctttt ccttttgatt
     6661 ccgttcaatt tttatcttca ttctttttcc tcttttctct tatttgtatc ggccagctcc
     6721 gataagatgc tgccgccaat agttttccgt tagattcgtc caccccactg gatctcctgc
     6781 atgaaacatg cgattctccc ctgcgtaaat gcccacatgg gtcacataag taccggcatc
     6841 ataggttgaa tgaaagaaaa ctaaatcacc tggtttggca tcttttagat caatatgagt
     6901 catggcatca tattgatcct gagccacccg aggaagtttc aggtcaactt tggcataaca
     6961 ccattgggtc aagccactac aatcaaaccc cgtttctgga cttgagccac caaacacata
     7021 ggccgttcct tcatacttaa atgcctcatc aaaaatttct tggactattt tgtcatcaaa
     7081 ctgagttgta gttaggtatt gcttcactaa taaacagtaa aacatattgc cataattata
     7141 ccgccaccca ccattttcct taatcgcgat tgggttgggg taatcgactt tttgaccacc
     7201 tgatttttcc tttgaaaaag attcagctaa agcaaaacta tgcttcttcc cgtttttccc
     7261 gacatgatct agatacccgc caccatagtt ataggcctga ataatactat cttgatcaca
     7321 ccctagttct ttggaacgct taaccaaaga cgcaaaatag agacagcctt gtttgatgga
     7381 tgcctcggta cttaatgaat taggtggtaa tccggctgac tcagaacttt gcataacgtc
     7441 ttccattgtg ccaccagatt ccacttgaat aatcgccaaa agaattggaa tatactctgt
     7501 tatatccgat tctttggcat acttttccac cattgcacgg tgtttcaata cttcttctga
     7561 gacactagtt ccgccaggag aagtgccaga actaccattt ccctcactgt cttcaccaaa
     7621 caacaaaccc acacacatca aaatacccat aaaagagatg gtcagaaaag ttaatgaaat
     7681 aacacccact ttcttcaagt tcattttttc tcacctgacc ttgattttgg acgatggttc
     7741 attctttttt gtactttttg attggtctgc tttggcagag tggacctctg tttcacttgt
     7801 tggaaagaat ttttcttact tgctgattgg cttttccctt ttgaaatgat ttcttgacta
     7861 gttagttttc gtttgatttc gggattcaga tttgctctca caatccgctt tggtgcttct
     7921 tttttcagca tcatatcttt ctgaggttga gctgctttat ctattggaat attcgccctt
     7981 ctttttccct gacgatttgt tgcttcactc agtatcttgc gtttttcgtc cattttcgca
     8041 tttcttcgtt gtcgttgttt ttctcgcaaa tccactcgat ccgctttttc ttggaacagt
     8101 ccgcgcttga attcttctgg tgcttttttc gacttttcga ttcctttatg gagattgtat
     8161 ttcaaattcg ttggcgtatt ccttacctga tcctttactt gtttagtctt atctttcacc
     8221 aaagcttggg tatccaaaac tttgccgatt tttctcccag ccaaattcat tcgggattgt
     8281 ttcttattat ttgaaactgg attctcctta gttaagtcag atagtatttc gtgatcgttc
     8341 ggtaatcgtg aattctttcg tagagaagaa ccggatggct taaatttgga ttttgattgt
     8401 tcctttgcca acttatttcc gactgctgca ccaccaccaa aagccaaagc tcgtcccaca
     8461 tttcgattga gttgccgcaa ttttcgattc attaacattt gcggttttcg catgacgcgt
     8521 cttcccattg actggctatc attactctgg aggttgaaca tactcataat gtcgcccaat
     8581 ttgaaataga taccggcaaa ggtcacaatt tgcagaaaag caaccatgaa aaatggatag
     8641 gttgccgaaa tattgaaaaa catcgtggag atgctaaatg cagtcgtaat caccaaagta
     8701 acacccgctc gcatcataat cgtattgaat aaacgaataa tcgccttctt tcctaaactt
     8761 tcataagtcg gcaacatgga caataagaaa ctaattggca gaaacatcgc aaagataatg
     8821 aagagaattt gcgagaacaa cataatccct gttagaagaa aaacaaagaa ggaaatgcca
     8881 atattgaaca ataccaaaaa gaccacggtc cctaaacggt tcatggtctt ggtaatactg
     8941 agattcgcat tgtcattgtc ttcaatctct gccttgaccg cttcttcccg atctttgcct
     9001 ttgttttcat ctggactcac agatagaatc ttgttgaccc ggtcttcacc aatttcttct
     9061 atgttggaat catcaaattg cagtagtaac caaggttgtt ggacttgaat cgaaaacaaa
     9121 ctatcccgaa tcaaatccac actatctttt ccttggcttt ctgaattggg tacaacgatc
     9181 tcggttccaa gggtaagggc cgcttcacta acatctgagc taaagtcatt gatttttgtg
     9241 atataggtag gcgcataagc aatgaacgaa cccgatagta gaaaaatcat cacaaaatta
     9301 atcacggctc gaactgcttt cgtggtttct cgttttaata atccggtata cgccacatac
     9361 acccccagag ctaaaatcat caataataga aacccaaaat aaaatccgga ggtctggagt
     9421 ccattttcag taataccagc caatgtttgg atattctttc caatactttc agccgtatcc
     9481 gaaatgaaat ctaatttgta agcctcttga accacataac ccgtggcgtt cgacaaatac
     9541 aaactcaccg tccaaatgaa attagtaatg gcataaagac catacatcac gcttttccct
     9601 aagccatcac cccaattcca aggcaaccaa tcccaagagc tatccacaaa gaaatctagt
     9661 tgatagttgt tcagtgaata tttggaatat agatttcctg cttcaactgt atcatcaact
     9721 agccctgtcg cctcggctac cgtaccaact aaactcaata gaactatcag agtgagtatg
     9781 ctaataaaaa cgattcccac aatccgaaaa agtttctttt tcatcccctc actccattct
     9841 ctcttgaaca ggtggacggg tatcaaaggc atggaagagt tcttcaaaga ctggatgtac
     9901 ctgaaccacg cctacccgac catacaaatc ctgcatcaag cattggccat tttccaaatc
     9961 tcgtaggcgt ttttgattgc tttcatcttc tcgatccaag ccaaagaatt ccaaggtttt
    10021 cttgatttca tgaatatcgg tactgcgaaa cgcaaacttc aacccgatat tattcttcaa
    10081 gcgttcatcc agtaaatcat cactattttg cgtgacaaag taaaccccgg cattcatagc
    10141 ccgcccagca cgaacgagct tattcgataa tgttttccct tgcgctactt gaagaaaact
    10201 ccacgcctca tctaagtcta caattttgaa gatttctcga ttactatgga taaagtccaa
    10261 ggcaaaagtg gaaatgacga ttagcatgga aacactaagc aattccatgg tggtatattc
    10321 ttcaaaagtg gtttctgcat ctggtaaaac caaatccgct acttgaatga tatttaactg
    10381 cctatctaaa ctaatcgact gggtaatcga accatctgag aaaagcaaat gggcaaaatc
    10441 ataatcggta aaggactcga tgtgatccgc gatattttcc gcaactggtg agccttcttt
    10501 tcgaagctca ttaattactt gtaataatcc ccgttgattg ctttgggtaa ccgtccgaat
    10561 ggccttacgc aacactggaa acttctcccc atctcgacta gaaattcctg ttaaaaaggt
    10621 cagaatatca attgctagac tttcagaatc cttctttttc tccataatca cataaggatc
    10681 aagtaatcct tgattttttg gcttactagt taagttgaga atattgattt catccgcaat
    10741 ttctggcaag gtttctttcc agccaccccg ttcccctttt ggatcgacaa tcacagcttg
    10801 tccaccaaaa aggacggcgt aataaactaa gagattgtta ctaaaggact ttccgccacc
    10861 gagtgaaccg aggaatgccg ctgctagcgc attggtaact gaatccttga ccccttgtga
    10921 ggccaatgcc ggtttcaggt aaacattgcg ccccgtatct acgttataac caaagtaaat
    10981 gccttccaac tcccctaaca tctgagtggc gccaaaacca agtccggcga gaaaatcaga
    11041 cgtaacatac tggatataat cattcatgta tcgcttacta gaaggtaaga attcttcatg
    11101 tagccccatc atatcgccaa aaggccggac tagttttaca ttggtatcat cataaaaatc
    11161 tagcacttca tcacaccggc gctttagttc atctactgat tcggcactta ctcgaaccac
    11221 atagctcagt ttgtacatgg attctttgga ctgatccaag gtagtttcca attcatctac
    11281 ggaatccaat gcatctaaga cattggaatt tgtttcattg tcagattgat aggcatggtt
    11341 gtccaagtct tttaattcct ttttcttatt gcgaacggta gcaagtgcct ttttgttcgt
    11401 cacaatctcc acattcatac tagtgtcaat tgggaatgta aattgttgtt gctggtaata
    11461 gaaaagttct gaggagggaa actccatttc ccccacaatt gtattaattg ttaagtacgc
    11521 tacatatgat tcatgattct catgttccat gcataagtac cggggctttt cttcaatcaa
    11581 acaacgactg ggacgtaata agtcgtaacg tttcaccaaa gtttccgact tcagcttttt
    11641 ctttggtagc tgaaattcat attcttcaaa tggtgtaccc ttttcgccat agatatgttc
    11701 aatcaaatac atcagatcac tcggtgtgac tcgtctgact ttaaatcttc gagccaattt
    11761 actttccatt agtttttcaa gcttggtata tcggcgaatt tcttcattgg ataaggaaac
    11821 aaaatcgccc atcaaatgat gattcactcc atagacaaat tcttgaaagc cggagaagaa
    11881 agatttcttg agattcttta gattgacctc ttcatctgtc gcaatcagtt tgaagccaat
    11941 aaaaaagcga taatcaatct gactatcgcc aatcatggat actaatgcct ctgtctgtaa
    12001 atcaattcgt tgtttcgcta cttctgttaa tcgcccggta atttcctttt ttgatcgttc
    12061 ttgcgtggca cgaatactac tttctgtggc aatttgtaag gcatgaatct tgccttcacg
    12121 attttgcgca atcaattgac gaaagttatc gtggacttga tatttctgtt caggtgacaa
    12181 aaaggagtaa ttgtaaggta ttaactcata gtaggcaaaa cattctccct cttgattaaa
    12241 gacaagatta ttttcaatat acttaatcgg gtacatagtt cacactcctt accagagtaa
    12301 tgggttcgtc ccatcgtttc ttctgttctt tgactttctt tcctgcgaaa gtgaccttaa
    12361 atcgtaagtg atagctaata aaagatcgta aaaatcccaa tggtttctta ccgtcaaacg
    12421 ttttttgcga catgaaccaa gtaactgcca ccggtatacc taagtatttt aaaaaggccc
    12481 cctctatcat agatagtgga ggcacatggg caaatacgat gacaaacagc aaggataaaa
    12541 caaaccaagc catctggtta aaagtcacag gaaaaggcag ttgaaagtca ttaattgcat
    12601 aaatgacctt ttcaactgcc caaatactgg tgtagctttt tattttcttc atcaaatcat
    12661 tcctttaaaa agaaggatag tttagaaatc aactaccctt caataatttt gttaatatgg
    12721 atactcaaaa acacctcgac tcgttaccaa ataattgcct ccaatctcca agtctctacc
    12781 acacgcatca aaatcaatat acgtcttcaa gtgttctggt acttctccaa aatcaccagc
    12841 ttctacaatc aagaaatatg ctacatcact catatcatcg caatctggat aatagataat
    12901 atcatccaca tgctctacca tctcctcaaa agaattgaag aaagcatgtt gaatctcaga
    12961 agcaacttca ccaactggcg ttccttcaag ttcctccgct aagccacaca accgattaat
    13021 ttcctcaatt ggcgtatact catcaatctc aaagggaagt tcataatcat gaattgcata
    13081 ttcctcgtat tcgtcattta acccaattcg ttctttcact tcatcaaaat cgacaggtgg
    13141 ggtaaaccac gcccccacca attcaccttc gttatatttc ccgaggttcg caatataaac
    13201 acgcatctgt tccatcgtgc ttccccctta cgcaccgaca attcggttaa acaaactgag
    13261 taagacatcc ttcaccccag aagcattaaa gaccaatccg accgcaatca aagcaatcac
    13321 caaaaagcca atcagtttgg aaaactcccg cttaaatcct aaaaacaaac caatgaccac
    13381 aattgccagt aagactaagg attg
//
LOCUS       DACDXO010000071        13396 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E23605_2017
            SAMN12639989-rid9881463.denovo.072, whole genome shotgun sequence.
ACCESSION   DACDXO010000071 DACDXO010000000
VERSION     DACDXO010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639989
            Sequence Read Archive: SRR10023389
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741914.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:42:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,972
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 33 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 17 of 73
            Pseudo Genes (multiple problems)  :: 10 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13396
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639989-rid9881463.denovo.072"
                     /isolate="E23605_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(15..707)
                     /locus_tag="IWM35_002305"
     CDS             complement(15..707)
                     /locus_tag="IWM35_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP9920136.1"
                     /translation="MQIELQHTHDKETFEKNYKVYFELARDSAMKYGIPLTLDTPYNQ
                     PGIKSHLWVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDDNPTTSEDKPVIDP
                     TRAGAANSTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELAR
                     VRADGIYRPDVNQAYNTLGNVGYHVSFNMRDFPNKKVYVMMRATNDPEGNTKGGAQDF
                     HDKRWYLNIPQR"
     gene            792..968
                     /locus_tag="IWM35_002306"
                     /pseudo
     CDS             792..968
                     /locus_tag="IWM35_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348541.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
     gene            complement(1144..1713)
                     /locus_tag="IWM35_002307"
     CDS             complement(1144..1713)
                     /locus_tag="IWM35_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAP9920137.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYETLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(1787..2110)
                     /locus_tag="IWM35_002308"
     CDS             complement(1787..2110)
                     /locus_tag="IWM35_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9920138.1"
                     /translation="MLLLKTLQERGYLDVHPPEVKIVFFFRYEHQEARRLAGVLNKTL
                     RHRKRISIHVCTRNKPPRYADLVIIDHFFPLDHNEDQKTYLYHPSFGIERLLTDINYW
                     LGKNR"
     gene            complement(2264..3076)
                     /locus_tag="IWM35_002309"
     CDS             complement(2264..3076)
                     /locus_tag="IWM35_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9920139.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFCFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESESKSKLTKVLYQE"
     gene            3430..4278
                     /locus_tag="IWM35_002310"
     CDS             3430..4278
                     /locus_tag="IWM35_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HAP9920140.1"
                     /translation="MIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGTFSRVQCTPD
                     LLPSDILGFSVYNSQSKEFEFRPGPIFTTILLADEINRTTPRTQSALLEAMAENHATI
                     DNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDDELLLLLDKF
                     EKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAIQLGISPRGS
                     LSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKKQIIHTLVAQ
                     IPIPVR"
     gene            4423..5364
                     /locus_tag="IWM35_002311"
     CDS             4423..5364
                     /locus_tag="IWM35_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HAP9920141.1"
                     /translation="MELDFIKQLSKETFGYLYIAATRLSQKKYAGYFFHSKKENFFFT
                     SPSFTNRLPSNLCRKKQEILILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFR
                     KSAKREISFPVTVLPAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNR
                     IDWKLSAHTQEWLYREYDYQEEECSPLFCFWGLPSPKFEPLLDLYFSLWHLRKEKQPE
                     LLILGNRAFHGKDPGHTYFASLEAGDEKAFFAHLKKYAKKQIVLFVPENSPEVSEAIE
                     TLSKDRTVYLVYFVEKQLMVQEKDKICSLPGGEWIDD"
     gene            5354..7525
                     /locus_tag="IWM35_002312"
                     /pseudo
     CDS             5354..7525
                     /locus_tag="IWM35_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343611.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
     gene            complement(7637..9808)
                     /gene="copB"
                     /locus_tag="IWM35_002313"
     CDS             complement(7637..9808)
                     /gene="copB"
                     /locus_tag="IWM35_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HAP9920142.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHEHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKKQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILA
                     IPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIR"
     gene            complement(9828..12014)
                     /locus_tag="IWM35_002314"
     CDS             complement(9828..12014)
                     /locus_tag="IWM35_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HAP9920143.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKTKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGLIAVADQIKLEVKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMNSSLASIAQTIELSRLTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLCLNRHMKK"
     gene            complement(12014..12223)
                     /gene="copZ"
                     /locus_tag="IWM35_002315"
     CDS             complement(12014..12223)
                     /gene="copZ"
                     /locus_tag="IWM35_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HAP9920144.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            complement(12236..12676)
                     /locus_tag="IWM35_002316"
     CDS             complement(12236..12676)
                     /locus_tag="IWM35_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HAP9920145.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQEGKKFIYYPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            complement(12750..13289)
                     /locus_tag="IWM35_002317"
     CDS             complement(12750..13289)
                     /locus_tag="IWM35_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP9920146.1"
                     /translation="MKIIVIGQSGSGKSTLARKIKEITDFPLLPLDLLWHTTDYSIQA
                     KKWFLQEQQLFMVSNPSWIVEGNYTSTLEERIKEADKIIWLKVPRYLAIYRVVWRSLK
                     RKINKKSRPDMPESFSEKLNREYLEFLSFIWHFEENNEPKIQQLIQVANARNKLIILK
                     TRREKQEFLTKLKIESKNN"
BASE COUNT         3763 a         2807 c         2381 g         4445 t
ORIGIN      
        1 gaggggggga ttttttatcg ttgtggaata tttaaatacc aacgcttgtc atgaaaatct
       61 tgtgctccac ccttagtatt tccttctgga tcgtttgtcg cacgcatcat gacgtatact
      121 ttcttattag ggaaatcacg catattgaaa gatacatgat agccaacatt tcctaaagta
      181 ttataagctt gatttacgtc tggtctataa atcccatcag ctcttactct agctagttct
      241 tttccagtat tgtagtccat aatgaaaata tactcgtatt tataattagc aatgtgccat
      301 ccagctacat gcaagtttgc gttttcgatt tctccgaact gatcaatgtg ggcgtaattt
      361 gttccatctg tcagcgtaga atttgcagca cctgctcgag ttggatcaat tactggttta
      421 tcctccgaag tagttggatt atcatccgta aaaccatgag ccaaatcata ggctagtttt
      481 tctttactta cacccatttc agaaagataa ccgtaaggat ctgtatgatc gccccaaata
      541 ttttgtgtta cccataaatg cgatttgatt cctggttggt tataaggagt gtctaatgtt
      601 aatggaatac catatttcat tgctgaatct cttgccaatt caaagtatac tttatagttc
      661 ttttcaaaag tttctttatc atgtgtatgt tgtaactcga tttgcacagg acttttggct
      721 cacgaggttg tcctactcta catcttgctg atagatatgc tgaaaggaaa acaccaacat
      781 tatgttttgc tttttcagct agaacgagac aagcaggaat acctttagaa gatatcaaag
      841 gttttttagg tcataaagat gtttccacta cacaagtcta tgcccatatt tctcccgaag
      901 tgaaaaaaag atcaatgaat caactagaaa actatataga agagcaaata aaaaagcact
      961 caaattgagt gcttttttaa gctcactatc actaaaacta tcactaattt tatttataag
     1021 cgaacatgtt tctatcactg attttttaaa tctagcgaaa aacccttatc ttaaaggcgt
     1081 tcgttcaatt ctttggcaag gtcttcaaag cctggcttgc ctagaagtgc gaacatggtt
     1141 ttcttatagc tcatgtagaa aaattttctt gatgatgatt ttatccatca attgagattg
     1201 actgttttgc tctcctatga tttcagctat gattgtccga ttttgcggtt ttcttcttaa
     1261 ctggtagttg attttcccag tttccatcaa ctcgctaagc atatccacga ctccgccaaa
     1321 tccggtcttt ttcgtaataa aacctagtgc taccattcgt tcatctagat tttttgctgt
     1381 aatcgtcaca ccagtaatga gcgagtgctg taatgtttgg gccagctctt ccatcccttc
     1441 agcattggat acgacggaag ctgttttgtt aataggttct tcgtttattt cttttggttc
     1501 tttcctctcc actttcactg tttgacgaag gacatctagt tcttgcaaga gcgtttcgta
     1561 ttgttcttgg gtcagcatca cgccagctac tttttcacga ttaaatatat atacgccagt
     1621 tcccgcgtct cgcgcttgag caaatacatc catcggcgat cgtttcactt ctgtgatcga
     1681 agatgtggga acttctaatt tctttaattc caatggattt cctcctttac taatcgcttt
     1741 ttattatagc acagactgac ttgttttgat cttttttcca aaggttttat cggtttttgc
     1801 ctaaccagta attgatatcg gtaagaagtc tttcgatacc aaatgaagga tggtacagat
     1861 acgttttttg atcttcgtta tggtctagtg gaaaaaaatg atcgatgatc actagatctg
     1921 cataccgagg cggcttattt cgagtacata catgtataga gatccgtttt cgatgtctta
     1981 gcgttttgtt cagtacccca gcaagtcttc ttgcttcttg gtgttcatag cgaaaaaaaa
     2041 agacgatttt cacttcagga gggtggacat ctaaataccc tcgttcctgg agtgttttca
     2101 gtaaaagcat cccccactct actgcggggt gtgcaagtgc ttgttggatc gctagccgaa
     2161 attgatatag tttatctttg gaattgggaa atttttctaa tgtttcctga taataacgaa
     2221 cttcttctat cagccggtca tggataccaa ttacgtaacg aagtcattct tggtaaagta
     2281 cttttgtcag ctttgatttg ctttcgctct caaattcgct attcagccaa aacagcgaat
     2341 caagcttttc agtcacaatc gtcaatttcc gatagtcttg tgtctcttct atccgtttcc
     2401 accgcttttc agtaatctgg ttacagctca agcgcaaaat tgctagataa tttttttctt
     2461 ttattggcaa aatctgctcc tctctaaata gcaattcagg aaacaattca aagaatagtg
     2521 ttcgactgtc tcctaaaatc tcgatttctg ccggatcctt tctttgcttc aataattgga
     2581 tgaacatctc agaaaaggac tgctctccat catctgcaaa aaaatgcgga aaatctttca
     2641 aaaagcaaaa cagcagatat cttacttttc tttctgaacc aaagatctgt gcttttttat
     2701 aagaaaaaga aaggtggtac tcgccaaacc actttctcaa tcgctcttgc gtagaacgat
     2761 cgtacttctg ctcgtaaggt tcattcaata gcagattttt cagtaaacaa aacgggggtg
     2821 attggatcag aaaaacaaac aagagataag gttcgctata tccttgatca taaactaact
     2881 gaacggtttt cttatgaaga atcagctgaa tgggacagtc gctttccgcc aaacgtttca
     2941 gatgccgatc cactgtttta acatcgattt tcagttcttc agctaaatca gcttttcgcc
     3001 agatcaaatc tttcattaga ttgattagtg aaaataaatt ctgtgcattt tttgataata
     3061 gtttttgcca tctcatcgat catttctcac ctgatttcta aacatattgt ttgatcaaaa
     3121 tgggttcact cctaaaaaga taaaaaactc cctttctatt tatttaaata cgcaaatctt
     3181 cctcattatt ttttatttta ctcaatttaa tcgtattctc atttcaaaac gaatgtactt
     3241 taaaaaaaca taataagatc actttgcttg tatgatgtat tcccttccat tacaatgaaa
     3301 taaatagcga ttttatgaga aggtgatgtg aaaatggaag aaaaaataga agaatccaaa
     3361 caaaaaatcg atcagttgat ataagaatta gaaaaagcgg tcatcagcaa acgttcaact
     3421 atccaattga tgatcgtggc cctattagca ggtggtcatg tcttattcga agatattccc
     3481 ggtgtaggta aaacgttact ggtcaaagca ctagcaaaag ctgtacaggg aaccttcagc
     3541 agagtccaat gtactcctga tctgctgcct agtgatattc ttggtttttc cgtatacaat
     3601 tcacaatcaa aagagtttga atttcgtccg ggaccgatct ttaccacgat cttgttagca
     3661 gatgagatca atcgtacgac tcctcgtact cagtcagcac ttttagaagc aatggccgag
     3721 aatcatgcaa cgatcgacaa caatacatac cctctagaca atcatttttt tgtcttagct
     3781 acgcaaaatc caatcgagta cgaaggaaca tatcctttgc cggaagcaca gctcgatcgt
     3841 tttttattcc gactacaaat cggttatcct tcttttgatg acgaactttt gctacttttg
     3901 gataaattcg aaaaagtgtt ggataactta aatgaagtat tgaacagtta tgaaatagaa
     3961 gaaataaaaa caaatgtggc gaatgtcttt gtcagtcccg aagtggcatc ttatgccttg
     4021 cagttagtgt cagcaacaag aagtcatgct gccatccagc ttggcatcag tccacgagga
     4081 agcctttcct ttattcaggc agcaaaagct tatgctttaa tccatgggag aaattacgtc
     4141 acgcccaaag atttgcagga tttagttcct tacgtgtttt cccatcgcct gattttttac
     4201 gatcgttcat tgaaggaaga cgaaaagaaa caaatcattc atactcttgt tgcacaaatt
     4261 cctattcctg tgaggtagat tgaggtagac aatggaaaaa atcaaagatt atttcattaa
     4321 ttttgtgtta tttctggtat atttcatcat tgtgcttttt gctcttgtat tcaacaattc
     4381 tttaggctgg ttcttgttgg ccttttttac tctttatgta tcatggagct tgatttcatt
     4441 aagcagcttt ctaaagaaac tttcggttac ttgtacattg cagccactcg tttatcgcaa
     4501 aaaaagtatg caggttattt tttccatagc aaaaaagaga actttttctt tacctctccc
     4561 tcgtttacaa atcggcttcc cagcaatctt tgcagaaaaa aacaagagat tttgattctt
     4621 acaaataaac cgcaagaagt ccaattgctt tggcagccaa aagaaagagg atgccatgaa
     4681 acattaactg tcatttttac agcctatgat agtctaggac ttttcagaaa aagtgcaaaa
     4741 agagagatct cttttcctgt gactgttctg cctgcttttt gtaagattca agcagaaaaa
     4801 ttacagagag ttctggaaaa aaaacagcag ctgaacgcgt accaaagagg tcttgatttt
     4861 cgggaacatc gcgcttatcg tccaggtgat tctttcaacc gaatcgattg gaaattgtct
     4921 gctcatactc aagagtggtt ataccgtgaa tacgattatc aagaagaaga atgttctcct
     4981 cttttttgct tttggggttt gcccagtcca aaatttgaac ctctgctaga cttgtatttt
     5041 tctttatggc atttgagaaa agaaaagcag ccggaacttc tgatattagg caaccgcgcc
     5101 tttcatggca aagaccccgg tcatacctat tttgcttctt tggaagctgg cgatgagaaa
     5161 gcttttttcg cacacttgaa aaaatatgca aaaaaacaaa tcgtcctctt tgttcctgag
     5221 aattctcctg aagtctcaga agctattgaa acattgtcaa aagatcgaac ggtttatctt
     5281 gtttatttcg tagaaaaaca actcatggtc caagaaaaag acaagatatg ttcattgcca
     5341 ggaggtgaat ggattgacga ttaaacggga aaaatggctg cgtggcgttt actcctttgt
     5401 gatactaagt gtaggtgctc cgatatttgt agtagctaat caattgcaaa acagctacct
     5461 tcttatccta actattggtg tcctatgcct tattgtctgc tgtattccta aaaaatgggt
     5521 acgtttgctg ctttctttgc cagtaatgat aggttgtttg tttttatatt ttccttcgaa
     5581 gacattttcc atattatgga tcttacagtt tttttcgtca gtcacagagg aattcttcca
     5641 tctatcccaa ggtaaacttt attatttgcc agaaaagaca gctttttttc tcataatgtt
     5701 gctgatctat ttatttatta cattgaccgt ggattacgac tattggtacg caccctttct
     5761 ttctttgatg gcttacttca tgatgcttac cgtttttcgg aaaaaagatt tactgtacga
     5821 aatgattgcg gtagtaactg ttttgttcgt gtatctggca gcaaggcaat ttttctccat
     5881 ccggttgttg tcgggaaaca ctcgattcca gtcattgact acagttctgc ttatttgttt
     5941 cttaactttt agcgctatct tgcctttata tcttccgaaa gtacaccaat ctctcgagga
     6001 aacgagtgaa ccgatccgag aatatcttaa tgatgaaggg ctttatgaca cactccatga
     6061 gtatcaatta accggaagtg caagaacagg cttcagtgag aatgatgaac agcttggtgg
     6121 acctttatat gataatggcg aagttgtttt tacagcgaac caaaaaggaa atggctattg
     6181 gaagatcgaa aataaaaatc attacaccgg ttctggatgg atagaagatc aggacgcacc
     6241 catccaacca atccaagaat tccctgttga catcacgagc taccaaaccg ttatagaaga
     6301 caaagaaaac attacattga ccctaaaaaa acaagttttc ttaccttatc cttatggaaa
     6361 tactaccttg actgattttt cattagctag tggacaaacg ctttactatt tgcctgaaag
     6421 ccagcgattc attcctgtcc cgaatgaatc gacacgttct gcaactttac aaacagaaag
     6481 gttagttttt acgagagaag aattaaaaag cagtcagtta aaagctgatg atttagaaaa
     6541 tcagcagaca aatcttcaat tgccaaaaag tttgcctaaa cgtattgagg acttggcggt
     6601 cgaattgacc aaaaatgaag caactttaat tcgtaaagtc gaagctgtct aacagtattt
     6661 aaaaacaagc ggaaatttcc gctattccaa aacggatacc gggcatactc caaaagaaca
     6721 cgattatgtg gattatttcc tctttgattc aaaagtcggc tattgtgaca acttttcaac
     6781 ttctatggtc gtccttcttc gaacacttgg tatcccagct cgctggacaa aaggttttac
     6841 aaatgggctc agaacaggca cagataatca agggaatgag gaatacagca ttctcaatag
     6901 tcatgctcat tcttggccag aggtatactt tcctggattt ggctggcttc catttgaacc
     6961 aaccccttca tttgccaatc caatgggttc acaaccagtt gctgaaacaa ccgaaaccgt
     7021 ccagcctgct atcgaacaac caaatgaaag cgaaaatatg gcagaaacta tttccagtga
     7081 gagatctgag gtcattccat cagaagaaac aacactcaca ccagcttata aaatcgtctt
     7141 attcagcctt ggagtgtgtt tatccgtctt gttgttcttt ctattgttat tcagaaaatc
     7201 tctgcgctgg acaagttatc ttatctatct tcattttttt gcttctacta atacgattgc
     7261 atatcaaaaa cttcttaacc ctttggaaac attggtaccg cgcaaacccg cagaatcttt
     7321 gcaggaatat agcctgcgaa tagaacaaga attcaagcta gatctgcgtt tcattcgatt
     7381 aactactcaa tatgaagcag aaatatatgg acgacaagca gatccggtaa aattcgataa
     7441 aaaaacctat ttagaaataa cgcatataat aagaaaaatg gtatacaaaa aaattctgac
     7501 aaaagggagc cgcttcaaaa aataagataa aatttctgga gaatctactt ctggaatgcc
     7561 gagaaccagc tgattgagta tcgattactc gaaaaaaagg ttgtgacacg ttttttgtca
     7621 caaccttttt tcattattat ctgattttca atgtcaaagc atttagagct acaactaccg
     7681 tacttaacga catcaagaca gcaccaactg ctggacttaa tatgatacca attggcgcaa
     7741 gaattccggc tgctagcgga attgcaagaa tattgtaacc tgccccccac cagaggttct
     7801 ggatcatttt ctttcgcgtt tgcttggcta gatcaagaaa atgcaaaata tcttttggat
     7861 cgctgtttgt caatacgaca tccgcagaat cgattgcgat atctgtccct gcaccgattg
     7921 ccatcccgat tgaagcacgg gctaaacttg gcgcatcatt gatcccatca cccaccatga
     7981 ttactttttt cccttggtca aggtatttct ggacaacagc ttctttatca tctggtaata
     8041 aaccgccata atattcttcg atccctaaat aatctgctac tgcttgggca gcgtttttat
     8101 tgtcaccagt aagcatgaca ggagtgattt ttctttcttt caatgttcgg ataaattctt
     8161 ttgcttctgt ttttactacg tcacctaaag caacgattgc agctagatga ttttctaaaa
     8221 tcaggaagct gatggtattt ccttgttctt ggtaaggttt caataatgct tgttctacgt
     8281 ttaatccgag acgctcaact tctttttcat taactatttt cacttgttgt tttttgactg
     8341 ttgcctctag cccgactcct gaaagatttt ttagattttc agcttgataa ggcttgattt
     8401 catgttcttt caaataattc ataatgccaa cagccaatgg atgattggca ttcgcttcca
     8461 aggctcctag atattgaagg atttcatttt tattgaacgc atcatcaagt acttcgacac
     8521 ccgttacagt aaattttcct tctgttagtg ttcctgtttt gtccagcatg atcacatcta
     8581 gatcattggc ttgctccagt gcattgcggt ttttcaaaag taatccgttt ttagctgcta
     8641 ttgatgtgga acgagcaaca actaatggga tagctaaacc tagagcatga ggacaggcga
     8701 tgataaagac tgttaccata cgttctaatg cattaggaag atctgtcaag aacaaccagg
     8761 cgatgaatgc caggataccc acaattaatg cgacataaaa cagccatttt gccactttgt
     8821 cagataatga ctctagcttg gatttttctc cttgtgcttt tcgcaccatt tccataacct
     8881 tcgctagata accattttca ccagttccag ttacttcgat ttcgatcgtt ccatccccat
     8941 tgatagaacc gccaatcact ttgtcattgg cttgtttttt gattcctctt gattcgccag
     9001 tcacagctga ttcgtcaaca attgtttcac ctttcaagat tacaccatct gttggcattt
     9061 tatcaccaga ccggacgatc aaacgatcac cttcgtttac ttctttcaat gaaactgtct
     9121 cttcttttcc atctttcgtc aagcgtttca ctgattctgg cagtaattct gctagttttt
     9181 gtaaagcatt gctggcattc gacacagcat tcatttcaat ccagtgacct agtagcataa
     9241 taacgatcaa agtagccaat tcccagaaga aatccatgac gtgtgtatgt ggattgagca
     9301 gatttgcgat aaacgaatag acactataaa tatatgcaac tgtaatcccc atcgcgatca
     9361 gtgtcatcat agcaggactt ttttgtttca attccatctt agctccactt aaaaatggct
     9421 gaccgccata gataaatagt atagttgcta agatcaatac gagccaatca gaaccaggaa
     9481 acgttacttg aaatggaaat tccattccca tcattggtga aaaaagaatg attgggatag
     9541 ctaggataag cgatagccaa aatttttgtt tgaaattccc catgtgcatg gaatgatcca
     9601 tgccgccgtg ttcgtgatcc atgtgcatcg aatggtccat ttgtttgctg tttgctatat
     9661 ctccatggtt atgtcccatg tgcatggaat gatccatctg actatggtca tgtttcatct
     9721 ccatctgttt ttcatgagat tctgtacttt ctttgtttac tacttctttt ttatgctcat
     9781 gttttttctc catatcgttc ccattcattt ttactccatc ccttctctta ctttttcatg
     9841 tgtcgattta agcacaggga gttcaacaat acactgacgg aactaaacgc catcgctcct
     9901 cctgcaatga ttggattcaa aaagcctagt gcggcaaaag gaataccaat cgtattatag
     9961 acaaaagccc agaaaagatt ttgtttgatt tttctcaatg tcaacctaga caactcgatt
    10021 gtttgtgcaa tggatgccaa actgctattc atcagcgtca catcggctgt ttccatcgca
    10081 atgtccgttc cacttcccat tgccataccg acatcagcta atgctaaggc cggtgcatca
    10141 ttgattccat cgcctgccat accgactttt ttgccatctt tctgaagttt ttctacgtag
    10201 gctgctttat cctctggaag tacttcagca aagatatgct ttggatcaat accaacttgt
    10261 ttaccaatcg tttcggctgc cagtttgttg tctcctgtca gcataaaaac atctaatcct
    10321 ttattctgga gctgcttgat tgcttgtttg acttctagtt tgatttgatc agccacggca
    10381 atcaacccga tcacttgctt ttcatcagct aaaaacatga ctgtttttcc ctgctgttct
    10441 aattctaacg cgtgttcttg gtattcatca aatgataggt tcaattcaat taatcgttta
    10501 cgtgtacccg caaaatagcg tactccgtta atcgtaccgc tgattccagc acctgggtgg
    10561 gctgtaaaat caatcatatc gtatgttgct gcttcttgca gttttccgta ttccacgatt
    10621 gctttaccta gtggatgttc agaagcttgt tctaaagtat aaaaaataga taggacttct
    10681 ttagaaccga ctagatctgt aactttgggc tttccttctg taatcgttcc agttttatcc
    10741 aaaacaatgc tgtctaagtg agcagctgct tctaatgctt ctccgccttt aattaaaatt
    10801 ccatttcgtg caccaacacc cgttcctacc ataatagctg ttggagtcgc taaacctaat
    10861 gcacatggac acgcaatgac taatacagag acactatgga tcaatgcgag ttgccaatca
    10921 ccagtgatca aacctgtagc tatgagcgtg ataaacgcga tgactaatac gatcggaaca
    10981 aaaatccctg agatccgatc tgcgattttt tggattgggg ctttgctccc ttgagcatct
    11041 tccaccattt gaatgatttg cgctaatact gtctgcttgc cgacttgtgt aacttccatg
    11101 tgaagtagac cattcgtatt gattgtcccg ccaaagagcg tgtcatccgg attcttttct
    11161 acaggaagat tttcaccagt aagcatactt tcatctatcg cactgcttcc tgaaataatt
    11221 cgaccatctg ctggaatctg ctcaccagga cggatgacta gttgatcacc aacgactact
    11281 tcttcaatcg ctaacgtttc ttctttcccg tttcttatca cttgggcggt ttttgtttga
    11341 agagacatca attgtttgat tgcattacct gttttcgttt ttgctgtatg ttccaaatat
    11401 ttgccgagca aaatcagcgt gatgatcata ctgctgcttt caaaataaag atcttggggg
    11461 tgtccacgga aaaatccgtt gtaaatgctc aatgcaaagg cagccgatgt tccaattgct
    11521 accaatacat ccatatttgg tgctttcgtc ttgattgcat ggtacgcacc tttataaaaa
    11581 cgagcaccga cataaaattg gacaggagca gacagcacta actggacgat tggaaaatga
    11641 aaaaagtgaa caatcgccgc atggcttcct aacatcatag caatcattga caacatcaac
    11701 ggcaaagtca aaaccgtact caatataaga tcgaatagca tttttttcaa atacgcttgc
    11761 ttttcttctg caattttttg tttgtgggct tcatcgtaca aaattgcacc atagccgatg
    11821 ttttctacgc tttggattaa tttttcagtg gtcgtccctt catatttgac acttgctttt
    11881 tcagtggcca agttgactgt ggcattgacc acaccaggtt gtccgttcag ctctttttcg
    11941 atcctagcag aacaatttgc acaagtcata cctgtaatga cgaacgtatc cattttggtt
    12001 tctgctgttg ccatcaaatc acctctgctt tataacccaa tccattgatt gcttgacaaa
    12061 tcttttcgga ttgaactttt gtttcatcaa acttgaccgt accgtttgct tttttcaaat
    12121 ttactttcac tttttgcacg ccgtcaagtg cgcttactgt ttcttctact cttgctgcac
    12181 aatgattgca gctcatacct tcgattgaaa attgttgttt cattttttct tcctcctatt
    12241 gatgttgttt acattcacat tgacctggga tacagttaca ttcgatcgta tctactggtt
    12301 gcttttgttc cagttgtttc aaaatctcat tgatatcttc ttgagtcaaa gtggcttctt
    12361 ctactagatc cgcaatcgtc tctccaatcc tttttgcaca gatatgggaa aaaagatttt
    12421 ccgtcgcact gcgaactgtt tccatctccg agacagctgg ataataaatg aatttttttc
    12481 cttcttgttc tgtccagatc acttcttttt ttaccaaacg acccagcaaa gttttgatcg
    12541 ttgctggttt ccaatccatc gaatcagcta agatctgcgt gatttcttgg gctgtcgtct
    12601 gtccaagtgt ccaaataaca cgcatgatct cccactctga atcactgatc tttactggcg
    12661 ttttgacttt ttccatttat ttcacacctt tcgtttacaa atgtaatcat tatcttatga
    12721 atacagatta cacttgtaaa caaaaactgt caattatttt tgctctctat tttcaattta
    12781 gttaaaaatt cttgtttctc tcgtcttgtt ttaaggatga tcagtttatt tcttgcatta
    12841 gctacttgga tcagctgctg tattttcggt tcattgtttt cttcaaaatg ccagataaaa
    12901 gataaaaatt ctaagtattc cctgtttagt ttctccgaaa atgattcagg catatctggc
    12961 cgagattttt tgtttatctt tctttttaga gaacgccaaa ctactcggta gatggcgaga
    13021 taacgcggca ctttaagcca aatgatctta tctgcttctt taatcctttc ctctaatgta
    13081 gaagtataat ttccttcaac gatccatgac ggattagata ccataaacaa ttgctgttct
    13141 tgcaaaaacc acttttttgc ctgaatactg tagtctgttg tatgccataa aaggtctagc
    13201 ggcagtagcg gaaagtcggt gatttctttg atcttccttg cgagggtcga ttttcccgac
    13261 ccactttgtc caatcacgat gatcttcatt cttctccctc acttctcttt ggaatttggt
    13321 tcgatcatac cacttataac gccgcaaaaa aacaagggac tgtgacaaaa gtcgtgtcaa
    13381 agtcccttgt ttcgaa
//
LOCUS       DACDXO010000072        13342 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E23605_2017
            SAMN12639989-rid9881463.denovo.073, whole genome shotgun sequence.
ACCESSION   DACDXO010000072 DACDXO010000000
VERSION     DACDXO010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639989
            Sequence Read Archive: SRR10023389
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741914.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:42:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,972
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 33 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 17 of 73
            Pseudo Genes (multiple problems)  :: 10 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13342
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639989-rid9881463.denovo.073"
                     /isolate="E23605_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            202..1077
                     /locus_tag="IWM35_002318"
     CDS             202..1077
                     /locus_tag="IWM35_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9920147.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPQFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKTKETVKKDDFNEK"
     gene            complement(1222..1374)
                     /locus_tag="IWM35_002319"
                     /pseudo
     CDS             complement(1222..1374)
                     /locus_tag="IWM35_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
     gene            complement(1642..2973)
                     /locus_tag="IWM35_002320"
     CDS             complement(1642..2973)
                     /locus_tag="IWM35_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP9920148.1"
                     /translation="MGKSSRKDIVFEKFGIVATKLGNQIHLRTLRDAFATFMPFMMLA
                     GFVTLINSVILEPTGFMGEIVDTSTLTTLQQIGTSIANGTLSITTLLVVAAVSYHMCA
                     SRNYTNHIAAVLVAISTFVVLTPMQMMFTPENADKAVEVTGVIPISYTGASGMFVGIF
                     VGLAATELFIKLSNNKKLQIQLSGNIPPAVLKSFNVLIPIIITITSFALLSFIIIQLF
                     NMDINKLITTIVTEPLSKITTGLPGFLLITSIANLFFGFGIHQAVISGSLLDPFLIQN
                     MQENMAAYANKEQIPHIINMAFKDTFAVMGGSGNTIALLIAIFIFSRRKDYKDFAKLS
                     VTPAIFNISEPIIFGLPIVFNISLIIPFVLAPIFSLTVAYFATAVGLINHVVVQIPWT
                     TPPVISGFLATAGDWRAAVLQILIIAASVFIYLPFLRIDEHVTTKLSENET"
     gene            complement(3122..3835)
                     /locus_tag="IWM35_002321"
     CDS             complement(3122..3835)
                     /locus_tag="IWM35_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016611336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP9920149.1"
                     /translation="MKKIAKYMEIYLDIKKKIVNGEYKIGEKLPTGDELAVIYHTSKL
                     TVKKGVDLLVSEGVLRSRSGYGTEVVRTPIDNSRVLGPNEGLFSVVGEEHVDSEIHTF
                     SIELPSKKVADKLSISVKDYVYNIVRSRFIDCQPYSIEQTFMPLAVIPGLEPKHLKKS
                     VYAYITNELGLEIKTSHIRIKGDTANEFDAKILKIDPGEFMIEIDKVVALSSGKPFEY
                     SVSRHLYQDFVFEAVFVEK"
     gene            4010..5434
                     /locus_tag="IWM35_002322"
     CDS             4010..5434
                     /locus_tag="IWM35_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015508962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP9920150.1"
                     /translation="MTTMKKLTFPDDFWWGSAWSAEQAEGKGETGKAETIWDRWFKEQ
                     PYRFYQRIDSSITTDHIHQYKEDIELMKKTGHNSFRVSISWARMFPNGGTGKVNPKAI
                     SFYRKLFSEMNNNGIKVFANLYHFDMPATLQDKGGWESREVVDAYVHYAETCFKEFGD
                     LVCHWFTFNEPLGPILGTYLEDFHYPNLIDFKRGAQAAFFTILAHAKAIQVFKELALP
                     SKIGVILNLSPTFPRSQNPADVKAAEIADLFYTRSFLDPMVKGNFSKKLILLLQEYNQ
                     LPEDYTKEDLVCIYENTAQILGLNYYEPRRVKSRSSMVDTSSPFLPEWFFEPHIMPGR
                     RMNEYRGWEIYEKGIYDLCMDIKNHYGNIEAFISENGMGVADEERFMDDTGQIIDDYR
                     IEYIKDHLAYLWKAINEGCNIKGYHLWTFIDCWSWINAYKNRYGLVSLDLTTQKRTIK
                     KSGEFYKQLSDTNGFRYDVDLLVE"
     gene            5733..6440
                     /locus_tag="IWM35_002323"
     CDS             5733..6440
                     /locus_tag="IWM35_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HAP9920151.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLIYAGSAQFITVSMLASHSPLLSIVLSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVILV
                     VTLIGCGLGVWIKHAFF"
     gene            6427..6750
                     /locus_tag="IWM35_002324"
     CDS             6427..6750
                     /locus_tag="IWM35_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HAP9920152.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRILPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLVGILSLS
                     LIRLL"
     gene            complement(6839..7876)
                     /locus_tag="IWM35_002325"
     CDS             complement(6839..7876)
                     /locus_tag="IWM35_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HAP9920153.1"
                     /translation="MSEKELMKVVGFYEGLSIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGAGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHIL
                     GKVVVSGPFNG"
     gene            complement(8197..9180)
                     /locus_tag="IWM35_002326"
     CDS             complement(8197..9180)
                     /locus_tag="IWM35_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HAP9920154.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKEPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILDTFEKEHPTLFSEQ"
     gene            complement(9595..10365)
                     /locus_tag="IWM35_002327"
     CDS             complement(9595..10365)
                     /locus_tag="IWM35_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9920155.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMISGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            complement(10358..11293)
                     /locus_tag="IWM35_002328"
     CDS             complement(10358..11293)
                     /locus_tag="IWM35_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9920156.1"
                     /translation="MANALEINNLKKVYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDTDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYETKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            complement(11609..13258)
                     /locus_tag="IWM35_002329"
     CDS             complement(11609..13258)
                     /locus_tag="IWM35_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HAP9920157.1"
                     /translation="MGQREFIVLIIIAAVILLVALDIFSRLKLKETVRSKWEKIPYQP
                     RFDKEESLKEAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDQQLEKLIAFYQENPQLREKIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGLGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKLPTPLQEELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAILNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLDYPETLVETAKTEAALFDEKRQWHVLG"
BASE COUNT         4134 a         2688 c         2295 g         4225 t
ORIGIN      
        1 ataaataaaa aacaaaaaaa gttccatctt taaaacacgc tattctttta ctttttctca
       61 cattctctaa gaaaagaaaa ggagaatgaa tttttgacaa ttcgagcaaa agtgattatc
      121 aaatatgtaa aatcagctac ttttttctta aacgtttctt tatactaaat ataaataagt
      181 gtttcatgaa aggagaacta gatgaataat tggcttgagt attttcctga aaacgttttg
      241 gaacgcggtt acagctatca tctgcacggt tttgtccgtc atttgaacta tacaagcaag
      301 tacttgtccg caacagtttc tggcacggaa gattataagg tagtgatcac ttgggatgaa
      361 aagaccaaca tgacttgtga ttgtctatat gccatagaag gaaaaaaatg caaacacatg
      421 gctgctgtct tattcgctta cgaagaacgt ccaataaaaa aatccaacta ctcactttcc
      481 gaactctcgt cccttgtttc ctccgctagt tcctctcttg tacgggagct tttaacagag
      541 attcttatag aacatccgca atttatcgaa cgcttcaaag ttaagatgcc atttcatgca
      601 ataaattatt cagataaatt aacaactatc atccataaat acgatcatat tatcaagaaa
      661 aacaaaaaca ggaaaacagc gaagttcatc atggagatgc gaaagttcat ccaagaagca
      721 gttgaatcgc tcattcagca aaatgcctat cttcccgcat tcgaattgat caatgaagtg
      781 attgctactc tcgaaacatt ctattgggag cctgaggatg aacgaaccct tcttctgata
      841 gaggactgtt attatttatg gaaagaatta ttagcagaag cacctcatgc agaaaaaagg
      901 caaatgttct cttggttcgt ctgccaagtc gatcatactg acgcctctta ttcaaaacgc
      961 tactccataa aaatcctgaa agaagacttt cgggaaaaag aattttctaa ccaaaagaaa
     1021 aaaatagata aaaaaactaa agaaacggta aagaaagacg atttcaatga aaaatagcaa
     1081 gtaaacgata acaaccatcc taaatccaca agaaatacag cagaccttgc tttaaaaaca
     1141 cgaatcatgc aaaggatgaa gtaaagaaga cttaacgcgt acccaatatt agtcatttga
     1201 ctattaggta cgcgttttgt tttataagtc atgacaaatt tccttcgttg ctgggtagat
     1261 tttccggcat tgttgataga ttttatgata cttttctaca ttttgtaaga gaggttccac
     1321 tgcatctgta tattggataa aattctgaca acaactttca ggtgtgtcga accactctaa
     1381 tctttaaaga taaatattaa aataacgatt aataatatta acagtcttta tttttcacca
     1441 ccaaaaacct atctttgcat atactaaata gacgtatata cacagatagg tttgtcttgt
     1501 gaaaaataat gattatcctt accaaaaaat gtttgttttt ctacatgttc gtacttatca
     1561 aaaaatatga tccttattga aattactggc tatttatatt ccaacgactt cttgtgttaa
     1621 tttttgttat ctcatagtaa attatgtttc attttcactt aatttagttg tcacgtgttc
     1681 gtcaattcgt aaaaacggta aatatataaa cacacttgcg gctataatta atatttgtaa
     1741 aacagctgct cgccagtctc cggctgttgc taaaaatccc gatataactg gtggcgtagt
     1801 ccaaggaatt tgcactacta catgattaat taatcccaca gcagttgcaa aatatgctac
     1861 tgtcaaagaa aaaatcggtg caagcacaaa gggaataatc agactgatat taaatacaat
     1921 aggcaatcca aaaataatag gttcgcttat attgaatata gcgggagtta ccgataattt
     1981 agcaaagtcc ttataatctt ttctacgact gaagataaat attgcaatca ataaagcaat
     2041 cgtatttccc gatccaccca ttaccgcaaa ggtatcttta aaggccatat tgataatatg
     2101 tggaatttgc tctttgtttg cataagctgc catattttct tgcatattct gaatcaaaaa
     2161 cggatctaac aatgaacctg atattactgc ttgatggata ccaaatccaa aaaatagatt
     2221 agcaatagat gtaattaaca aaaatcctgg taaaccagtt gtaattttac ttaatggctc
     2281 tgtaactatc gtggtaatta gcttatttat atccatatta aacaattgaa tgattataaa
     2341 tgacaataaa gcaaacgagg taatcgtaat aatgattggg attaatacat taaaagactt
     2401 taaaacagcg ggaggaatat ttccggatag ctgaatctgt agtttcttat tattagaaag
     2461 tttaataaat aattctgtag ccgctaatcc aacaaatata cctacaaaca ttcctgaagc
     2521 tcctgtatag gatataggta tcacacccgt cacttcaaca gctttatccg cattttcagg
     2581 agtaaacatc atctgcatag gtgttaatac aacaaatgtc gaaatagcaa ctaataccgc
     2641 tgcaatatga tttgtataat ttctgcttgc acacatatga taagatactg ctgctacaac
     2701 taataatgta gttatgctta atgtaccatt ggcaattgaa gtaccaattt gttgtaacgt
     2761 ggtcaatgta ctagtatcga caatttcccc catgaatcct gttggttcta agatcactga
     2821 attaataagt gtcacaaatc ctgctaacat cataaatggc ataaatgtag caaacgcatc
     2881 tcgcaaagtt cttaaatgga tctgattccc caacttagtc gcaactatac caaatttttc
     2941 aaatacaatg tccttcctgc tacttttgcc catatttttt ctcctttatg cttattgtaa
     3001 tcgattactt tttccaccca aataatacat cacttatcaa aaaaagtaaa tacttttaat
     3061 taaatatata tatctttttt agtataaaaa aataagctct gaaaaaataa ttttcccgag
     3121 cttatttttc aacaaaaaca gcttcaaaaa caaagtcttg atataaatgt ctcgataccg
     3181 aatactcgaa aggtttacca gacgatagag caacaacttt gtctatctca atcataaatt
     3241 cacctggatc tatttttaat atttttgcgt caaattcatt cgcagtatct cctttgatac
     3301 gtatgtgcga agtctttatt tccagaccca gttcatttgt gatataagca tatacagatt
     3361 tctttaaatg ttttggttcc aagcctggta tgactgctaa tggcatgaat gtttgctcaa
     3421 tcgagtatgg ttgacaatca ataaaacggc ttcgtacaat attatataca tagtctttta
     3481 cactgatact taatttatca gctacttttt tcgatggaag ttcaattgag aaagtatgga
     3541 tttctgaatc tacgtgttct tcaccaacca cgctaaacaa accttcgttt ggaccaagaa
     3601 ctcgtgagtt atcaattggt gttctgacga cttcagtccc atacccactt ctacttcgca
     3661 aaactccttc ggaaacaagt aagtctacgc ctttctttac tgttaacttg cttgtatgat
     3721 aaataactgc caattcatct cctgtaggaa gtttctcacc aattttatac tctccattta
     3781 caattttttt ctttatatct aagtaaattt ccatatactt cgcaattttt ttcaatggtg
     3841 accctccaat caaaaacata tcttcatcta tactttaaca taaataaacc gataaaatcc
     3901 acatcaaaat tatacacaca ataaaaaaga tatatatatt taaacaaaac tatttacttt
     3961 aagttgtaag cggtgttatc attaagtgga aacaggaggt aaaaaaatta tgacaactat
     4021 gaaaaagcta acttttccag atgatttttg gtggggttcc gcttggtcag ctgagcaagc
     4081 agaaggaaaa ggagaaacag gaaaagcaga gactatctgg gatcgctggt ttaaagaaca
     4141 accttaccgg ttttatcaac gaatcgattc ctctattaca accgatcaca tccatcaata
     4201 taaagaagat atagaactaa tgaaaaaaac tgggcataac tcctttcgcg tttctatttc
     4261 ttgggcacga atgtttccaa atggcggtac cggaaaagta aatcccaaag ccatttcttt
     4321 ctatcgaaaa ttattttccg aaatgaataa taacggaatc aaagtatttg ccaacttata
     4381 tcattttgac atgcctgcta ctcttcaaga taaaggagga tgggaatcac gtgaagtagt
     4441 tgatgcttat gttcattacg ctgaaacttg ttttaaagaa tttggtgact tagtttgtca
     4501 ttggtttacc ttcaatgaac cacttgggcc tattttaggt acttatcttg aagattttca
     4561 ttatccaaac ctcattgact ttaaacgcgg agcgcaagca gcattcttca ctatacttgc
     4621 acacgcaaaa gcaatacaag tatttaaaga actagctctt cctagtaaaa taggcgttat
     4681 tctaaatttg agtcctactt ttcctcgtag tcaaaatcct gctgatgtga aagcagcaga
     4741 aatcgctgat ttattttata cgcggagctt tttagatcct atggttaaag ggaatttttc
     4801 taaaaagctt atcctgttat tacaagaata taatcagctt cctgaagatt atacgaaaga
     4861 agatcttgta tgtatctacg aaaataccgc tcaaattctt ggactaaact attacgagcc
     4921 acgtcgcgtc aaatctcgtt cttctatggt ggatacatct agtccatttt taccagagtg
     4981 gtttttcgaa ccacatatca tgcctggacg acgaatgaac gaataccggg gatgggaaat
     5041 ttacgaaaaa ggaatttatg atttgtgcat ggatatcaag aaccattatg gaaacatcga
     5101 agcatttatc tcggagaacg gaatgggcgt tgcggatgaa gaacgtttca tggatgatac
     5161 agggcaaata attgatgatt atcggattga gtatatcaaa gatcatcttg cttacttatg
     5221 gaaggcaata aatgaaggat gtaacattaa ggggtatcat ctttggacat ttattgattg
     5281 ctggtcatgg atcaacgcct ataaaaaccg atatgggctg gtttcactcg accttactac
     5341 tcaaaaaaga acaattaaaa aaagtggcga attttacaag caattaagtg atacaaatgg
     5401 atttagatac gatgtagact tgttagttga ataatattct aaaattccac ttgaagtgaa
     5461 cacattagaa aacaagaata atgagaaata ccaataacaa atactttttc ttcatacttc
     5521 atttatactt gtcagtataa gagactgtga caaatgtagt gatcctcaga agttaggtct
     5581 ggaaatctaa cttgagaaga tttttttaaa ctacgtccag tcttttttat ttgtttaaaa
     5641 ttgaagttga caagtcttca ttaaaaattt agaatattca caagtttgac gacaattatg
     5701 aaaatttatg tcgtgctaaa ggagttttgt tattggatga aaagttagat ataaaaactg
     5761 cgatcaaaga cacgttacct accgtttttg gttatatcgg tattggactt gcatttggta
     5821 tcgttgggaa agctgccggg tttcatccgc tggtcgtcac gttgatgtcc ctgctgatct
     5881 atgctggttc tgcccaattt atcacggtca gcatgcttgc tagccatagt ccactgctct
     5941 ccatcgttct ctcgaccttt ctagtcaatt cccgaatgat tctgatgagt atgacgattg
     6001 ctccttattt caagaaaaat cggctgcttc aaaatctgct gattggcaca ttgctgacag
     6061 atgaaagttt cgcactggga atgaataaat tgaactatac tggacaaaag ttaaactttc
     6121 gttggatgaa tacagctaat tggatttctt atttgacgtg ggtgggttct tctcttgttg
     6181 gcgcactttt agggaatttt attactgatc cgaaaaagtt tggtttggat ttcgccatcg
     6241 tcgctatgtt tatcgggtta ctttatcttc aagtcatttc tgatagaaac acctctaaac
     6301 gattacagtt gatcctgatt ggtctcactt tgattctggt atatgtcgga ctgattttta
     6361 tcccaagtaa cttagtgatt ttagttgtaa cactgattgg gtgcggattg ggggtgtgga
     6421 tcaaacatgc cttcttttga gtttacattg ctgactattt taggttgtac tttagcaact
     6481 tggctttcta ggatcctacc atttgtctta ctgaaaaaat tcgatttgcc gcaaccgttg
     6541 cttgagtatt taagttttgt cccgatcgtc attatgtctg ctttatggtt cagcagtcta
     6601 tttacccaaa atatcggaca tctgccgcag atcaatctcg aaaatgcttt ggcttcagtt
     6661 cctacacttt tagctgctat tctttcaaaa agcttattgg tgatcgtctt ggtaggaatt
     6721 ctttcccttt cgttgattcg cttattataa ataaaaaaac aagagcttat ggcaaattac
     6781 tgtcatgcgc tcttgttttt atcaaagtca aataagaaaa aaatcggcgt attcttgctt
     6841 atccgttgaa cggaccacta acaacgactt ttcctaatat atgtcctgtt tctacgtctt
     6901 tggttgcttg tttcaagttc ttcgcattca tcccatctga atagaccttg ctcaatgtac
     6961 tgcggataat cccttcattt gctaaagcag caatgtgcgc taaagcttct ccttgcgttt
     7021 cgatttcata gttatagtct gttttagcga acatatactc ccagtcgaag ctgatagatt
     7081 ggtttttcca aacacctata tctaacggct ctttgatccc tacgatcgtt cccacatgtc
     7141 caaatggtcg gacaaggtgt ttgatttgat caaagtagcg ggtaatatcg aataatacag
     7201 cgacatactc tacttgatcg attcctaatt cacgaaaact tttacttaag tcgtgatgat
     7261 aatcgattgg atagtctaca cctgtttttt tcagccattc aaaattcttc ggacttgcag
     7321 tcgcgtagac ttctaggcca gcccaatgcg ctagttgatt caaaatcgaa ccgacgcccc
     7381 cagctccgtt gatcactaag atttttttcc cgcggttggc attttcttct ggagtcagac
     7441 caaatttttc aaataacaat tcataagcgg tcaatgaggt caacggcaag gctgcggctt
     7501 cttcatctga aagatttttt ggtgcaagag cgacaatacg ttcgtcaacc agttggtatt
     7561 cttgattgct tcctgctctt gtcgttgtac ctgcgtaaaa aacgcgatcg ccgacagaaa
     7621 atttttgcac ttcatcacct acagcaacaa ctttcccgac tccatcaaac cctaagatac
     7681 gtaaagcatt tcttatcccg ttatcttggc gaagtttcgt atcgacagga ttaacagata
     7741 ctgcgttgac ttttaccaga agatcccgtg aactgggtac aggtatctgt tctttttcat
     7801 caataaagct ttttggatca tcgatcgaca acccttcata aaaaccgact accttcatta
     7861 attccttttc tgacatacga attcctcctt agcttcatca agttggagca tccatacagt
     7921 tttcatttca atatatatag tatgctattt gttccacttt tattcatcat aattattctc
     7981 ttacttaaag taaactattt gcactttttt ggttaaaaaa gaggttgcga caagactttt
     8041 gtcacaacct cttttaaacg gtgttcaatc aactgaccct cggtattcgc tcaaaaaacg
     8101 acaaaatatg aggagctatt tttgtttggc tgttcttatt acttgatgca ggacagcttt
     8161 ttcacgacct ctaattcacg gtgttcaagc aagaaatcat tgttcactga aaagcgttgg
     8221 atgttctttt tcaaaggtgt ctaagatctc tcgtacttca tcagtagatt tcgtattcat
     8281 caactgaact cgcaagtcat ttgctcctgg aaatcctttg atatagattt taaagaaacg
     8341 atgtagtcct gtgatggaac gcgggatgat ttctgaataa tgatcttgca gatctagttg
     8401 cagacgaagt agtccgatca gttctttttc tgtatgttcc ctcggttctt tttcaaatgc
     8461 ataaggattc ttgaaaatcc cccggccaat catgatgcca tctacgccat atttttcggc
     8521 caattcttct cctgtctggc gatctgggat atctccgttg atcgtgatca gtgtttgcgg
     8581 agcgatttgg tcacgtaaag caatgatttt agggatgact tcccaatgtg catctacttt
     8641 actcatctct ttacgcgtac gcaaatggac agaaagattg gctatatctt gatgaagtag
     8701 gtgactgatc cattcttcca tctcagacat ttccgtatag ccgatacgcg tcttgacgct
     8761 gacaggcaaa ccgccagctt tagccgcttc aatcaattgt gccgctactt ctgggcgaag
     8821 gatcaagcca ctgccttttc cacgttctgc tacattaggg actgggcagc ccatgttgat
     8881 atcgattcct tggaagccca tttctgccat agcaatgctc atttctctaa aaaattctgg
     8941 cttgtctccc caaatatggg caaccatcgg ctgttcatct tctgtaaagg tcaatcttcc
     9001 tcgtacactg tcttttccat ctggatgaca gaaactgtct gagtttgtaa actcagtaaa
     9061 gaagacatcc ggtgctgctg catgctttac gacatggcga aatacaacat ctgttacatc
     9121 ttccattggt gctaaaacaa aaaaaggctt tggcagctct ttccaaaaat tagtagtcat
     9181 ttatttgtta ctctccattc tatcaagcag tgaagtcgca atctttctca aaaacattgt
     9241 cattatacta ggaaacaaaa aaagaagcaa agtgaacagc ttaaaagatg tttctgattg
     9301 agcaaaaaaa gactgtgaca aaaatcttat cacaattcct tttccggaat aaatggtgtt
     9361 caaaagcttg catctgcggt aataagtcca accaaacgaa aaataggagg agctattttt
     9421 gtttggctgt tcttattact tgatgcagaa cagctttttc acaacctctc cactaaacgg
     9481 tgttcaatca gctgaccctc ggtattagct caacaaacga caaaatatga ggaactattt
     9541 tcccgtttgt ttctctaata ctcaggtcaa aacgctgatt tcacaacctc ttttctaact
     9601 tctcaatcct cttcctttat tgatcaaata ccaagtaacg gcgtacaaaa caacgatgaa
     9661 tagcacaagt acgatcatcg atgttgctac tggcacatcg attgttccaa ggaatccgta
     9721 acggaaaccg gagatcatat aaacgatcgg attgactttc gaaacagctt gccaaaatgg
     9781 cggcaacata gagatggcat agaaaacgcc acctagataa gttaacggct gcaagacaaa
     9841 tgtaggcacg atcgatacat cgtcataaga ctgagcaaaa atcccgttga gcagtcctgc
     9901 taacgaaaac aagattgccg tcatcaataa tgtaatgatg accatgaacc atgaatacac
     9961 atgaagtggt acaaagaaca aggaaatgat cgttacgaga gagccgacga gaatactgcg
    10021 acccactccg ccaatcacaa agccccaaat gatcacatgc gtaggaacag gcgctaccaa
    10081 gatttcttcg atgtttttct ggaatttttg agagaagaag gacgaagaaa cgttggcata
    10141 cgaactagtg atagcagaca tcataatcaa accaggcacg atgaattcca tatatgaaaa
    10201 accacccatg tcaccgatcc gaccaccgat catttttccg aaaataacaa agtacaagga
    10261 tgtcgtgatc acaggcggaa ccagtgtttg gacccagata cgcaaatagc ggttcgtttc
    10321 cttgatagct aaacttttta atgcagtaaa ataaagatta aacatttttc tcctccgtat
    10381 gattattttc ttctgtgatt ttcaaaaaca gttcttctaa acgattcgac ttattcctca
    10441 tagaaagaac tttgattcct tgtcttgtca gctgatcgaa gattccgttc accccttgat
    10501 ttcgttcaac ttcgacagcg attgtctgtt catcttcaaa tgtacaagcg tatccttcaa
    10561 tcacaggttt ggtctcatat ggcgctaaat caaagataaa tgtttcaaat tgcagtttgg
    10621 atagaagctc tttcatacta gtattctcga tcagttcacc tgattggatg atcccgatgt
    10681 tgcgacaaag catttcagct tcttctaaat aatgcgtcgt caaaatgatc gttgtaccat
    10741 tttcgttcaa ttctcggaga aactcccaca tctcgcgtct caattcaata tcgactcctg
    10801 ctgttggttc atccaagatc agcaaacgtg gttcatgcat caatgcccta gcaatcatca
    10861 gtcttcgctt cattcccccg gaaagcatac gtgcacggac atttctcttt tcccaaagat
    10921 tggactgctt caaatatttt tcgctgcgtt tcatcgcttc tttgcgagag acaccgtagt
    10981 agcctgcttg attcacgacg atttgctgga ccgtttcaaa gggattgaaa ttgaactctt
    11041 gcgggactaa tccaatctgt tgctttgctc ggactagatc cgtatccaga tcataaccga
    11101 agacattcac ttttccagat gttttattga ctaaagaagt aatgattccg atcgttgtag
    11161 acttcccagc tccatttggt cctaatagcg catagaaatc tccttctttc acggaaagat
    11221 cgattcctcg caatgcttcg accccacttc cataaacctt tttcaaatta tttatttcaa
    11281 gcgcatttgc catttatttc ctatctcctt atagacatga ttctttattg tatcatggaa
    11341 tacagcagat gaaaaaacaa cttggttcca gcgaaaaatc aggttgctgg gctcaggaaa
    11401 atctcctaat gaaagacatc aatcggacga agtcagacaa ccttttcaga aaaaacaaat
    11461 cgaagataaa ggattactat tcatatgtgc aacaaaagga gcgtgagaca aaagtaaaga
    11521 acacttttgt ctcacgctct aaacacgtat aaacggcgga agcaaaagcc aagatttttg
    11581 atccaataaa tactttcgat cacttcgcct atcccaatac atgccactgc cgcttttcat
    11641 caaataacgc ggcttccgtt tttgcggttt ctactaaagt ctctgggtaa tccaaaactt
    11701 tcagcaaggc aatcgcattt cgggtccttg aaggtccttc ttttaatttg aaatcaaaac
    11761 tgattccttt tccttctgtt acttgttctt caaagtggac attctcacaa taattcttta
    11821 aaatctctgt caattcgata tcatgtgtgg cgacaaatgc taaagatgga tactccgcaa
    11881 gccaccgaac gactgaagcg gaagaagcaa tccgttcgat cgtatttgtc ccttttaaaa
    11941 tctcatctac gaagcagtag cagcgttctt tggaagccac ttggtccaac aaacgtttga
    12001 tcgatttgat ttctgaaaca aaataactgt cgccttcaaa taaatcatct tcgattgcca
    12061 tggaagtcaa cacatgtcca ggttgcaggg taaactgttc tgcaatcgct gtttggatcg
    12121 tctgtgctag aatacagctg atggctatac ttttgacata cgtagatttt cctgatgcat
    12181 tggaacccgt gactaatgta ttcttctgcc agttgaccgg attcactacc gcttctttta
    12241 acaatggatg atagatatct ttcgctgtca caccgccctc agtaaactct ggtcggcaag
    12301 tgatgggcat ataagtacgg taattcaata ttgcagcggc tacttctaat tctcccagca
    12361 attcccacag cttgatggct tcagttgaat gcttttctaa tttagtgaga acgaaatgat
    12421 aggagataaa tggcagcatg aacatgatgt tgacatattc aaaaatcaac tctgcttcac
    12481 tcccactctt catacgaaat gaaaaaccta atttagggat gtctcttaat ggtttagttt
    12541 gctgtttcag ctcttcttga agtggtgtcg gcagttttgc tagctgattg gcggctgaaa
    12601 tcgttgaaac caaataactc atgctattca gctctgtttc tagtgtttgt ttcttcacta
    12661 gataatagac cgtattgaat actaacccac cgattgccaa atagattcct agtacttgtc
    12721 ctaaaagaag caaacccaaa cccaccagcg gaagcaagcc aagaaaaata aataaaccta
    12781 cgcgtccaat ctttttactt tttccatccg ctagatattg ttttgcaaaa ttatgatctt
    12841 tttttcctaa acgagcgaat tgatattgga ttttttctct cagttgagga ttttcctgat
    12901 aaaaagcaat cagtttttct aactgctgat cttcaccaaa atcaaagctg cgtaaacgtt
    12961 gatacaacgc ttctgaacct acactagaat aggtactgtt gattccttcg aatacttcga
    13021 acatatccag gtcataccaa gtaagatcat cgatttcgct atcccatgaa cggaacttct
    13081 tctccgtgag ccatgcttcc ttcaagcttt cttctttatc aaatctcggt tgataaggaa
    13141 ttttttccca tttgctacga actgtttcct ttaatttcaa acgtgaaaaa atgtctaaag
    13201 caactaataa aataacagct gcaataataa ttaaaacgat aaattctctt tgacccatat
    13261 ccctctccgt tcctttattt gttagcttta gtatagcact gttgcgctat ttgaaaagag
    13321 ggtaaaaaaa cacctttcaa aa
//
LOCUS       DACDXO010000073        12961 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E23605_2017
            SAMN12639989-rid9881463.denovo.074, whole genome shotgun sequence.
ACCESSION   DACDXO010000073 DACDXO010000000
VERSION     DACDXO010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639989
            Sequence Read Archive: SRR10023389
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741914.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:42:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,972
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 33 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 17 of 73
            Pseudo Genes (multiple problems)  :: 10 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12961
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639989-rid9881463.denovo.074"
                     /isolate="E23605_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            139..477
                     /locus_tag="IWM35_002330"
     CDS             139..477
                     /locus_tag="IWM35_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9920158.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(682..1257)
                     /locus_tag="IWM35_002331"
     CDS             complement(682..1257)
                     /locus_tag="IWM35_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF159 family protein"
                     /protein_id="HAP9920159.1"
                     /translation="MCGRYFFDLESELLQNYYREAQTKQPKQAAELAAGEVFPSNLVV
                     TLRKEEENILTPEIMKWGFTGFKKGQLMINARAETVEEKKTFHQPFLQNRCVFPMSGF
                     YEWNEEKDKFMFSNKEKIVYIGGFYRSHQDEKMAESIIMTTEPNRTVAPIHDRMPLVL
                     TEEQIEPWVTDISFARKIITQQMPELVMEKV"
     gene            complement(1989..2570)
                     /locus_tag="IWM35_002332"
     CDS             complement(1989..2570)
                     /locus_tag="IWM35_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP9920160.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(2753..3379)
                     /locus_tag="IWM35_002333"
     CDS             complement(2753..3379)
                     /locus_tag="IWM35_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP9920161.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(3401..4732)
                     /locus_tag="IWM35_002334"
     CDS             complement(3401..4732)
                     /locus_tag="IWM35_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HAP9920162.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(4887..5288)
                     /locus_tag="IWM35_002335"
     CDS             complement(4887..5288)
                     /locus_tag="IWM35_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HAP9920163.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(5374..5580)
                     /locus_tag="IWM35_002336"
     CDS             complement(5374..5580)
                     /locus_tag="IWM35_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9920164.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(5675..7177)
                     /locus_tag="IWM35_002337"
     CDS             complement(5675..7177)
                     /locus_tag="IWM35_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HAP9920165.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(7195..7650)
                     /gene="argR"
                     /locus_tag="IWM35_002338"
     CDS             complement(7195..7650)
                     /gene="argR"
                     /locus_tag="IWM35_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HAP9920166.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(7748..8365)
                     /locus_tag="IWM35_002339"
     CDS             complement(7748..8365)
                     /locus_tag="IWM35_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9920167.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKPFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQLVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            8586..9959
                     /locus_tag="IWM35_002340"
     CDS             8586..9959
                     /locus_tag="IWM35_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAP9920168.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLILSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            10034..11146
                     /locus_tag="IWM35_002341"
     CDS             10034..11146
                     /locus_tag="IWM35_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HAP9920169.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWSTEDYQKDISTYITKNPS"
     gene            complement(11218..12855)
                     /locus_tag="IWM35_002342"
     CDS             complement(11218..12855)
                     /locus_tag="IWM35_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HAP9920170.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAAVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
BASE COUNT         3506 a         2749 c         2311 g         4395 t
ORIGIN      
        1 actaaaaaaa taatccaaga aacattgaac ctatggatcc aaaagctacc caaatactat
       61 agctaacccc ccgctacctt caattttgca gataaaacgc tttgggagaa aatttcaaag
      121 acaacaggcc atcctatcat gcccgattat aaacaaaaag ctggctattc cttttgctct
      181 gacaaatttc aattgggaaa gttcacgaaa ctagcaggtg aagaagtttc aactgttcgg
      241 atcaaagaat gcccgcttca aatagaaaca ttggttcaac gaatttcaac tagacaagac
      301 tttgctattg tagaacgtga gatcgtagct atttttgtag aagaagggat tttacatgat
      361 ccaactcata tcaatgtgga taaatggcaa cctcttatct ataaattcag agaatataca
      421 actgccaacc agtcgttaac tttgaatttt agattccaag aattcaaaga gtcataatga
      481 taaacatcgt taggtataaa taaaaaagac tcgcagcacg agtaaaaaac aaatagaagc
      541 tatacattag aaaggtcgac tgtgacccta tagatgcagt atatgcagct tatgagcatt
      601 tcttctgttc tctttgtttt tgggatctgt gctgaagtct ttttcgtttt ttgccgctct
      661 ttctttgaaa aatcgattat gttatacctt ctccattact aattcaggca tctgctgagt
      721 aatgattttt cgcgcaaaag aaatatctgt tacccaaggt tcgatttgtt cttcagtcaa
      781 gacaagcggc attcggtcat ggattggtgc gactgtgcgg ttaggttcag tagtcattat
      841 gattgattct gccatttttt catcctgatg gctacgataa aaaccgccaa tatacactat
      901 tttttcctta tttgaaaaca taaatttgtc tttttcttca ttccactcat aaaagccact
      961 cattgggaaa acacaacgat tttgcaaaaa tggttgatga aaggtctttt tttcttctac
     1021 tgtctctgca cgggcattaa tcatcaattg tcctttttta aatccggtaa atccccactt
     1081 catgatttcc ggagtgagga tgttttcttc ctcttttctt aacgtcacaa caagatttga
     1141 cggaaaaact tctccagctg ctaattctgc tgcctgcttc ggttgtttcg tctgggcttc
     1201 tcgataataa ttctgaagga gctctgactc tagatcaaaa aaataacgac cacacatcct
     1261 aatccctccc aatctttttt tctatcatac actgcaacaa gaagttcgca ccaacaaaac
     1321 gctttcaatt tttgtagaac taaaaaagtt gtgacaagtg acaaactctc cagtgaatgg
     1381 tattctcctg ccaactcctc ggtcacaagt cacaatattc aacaaacatg agaagctgct
     1441 ttttgaaatt gttccttgtg actcggagtg aaacggcagg cttacgacct ctgggtaaac
     1501 ggtgttcaaa agccagtttc tcggtattaa gccaaattta tgcaaaaatg tagaaagcca
     1561 ttttcacaaa atttttctta atactcgaaa ctaagcgact ttttcacaac ctcttcctta
     1621 aacggtgttc aaaagcttgc atctgcggta ataagcccaa ccaaacgaaa aatatgagca
     1681 gctattttta tttggctgtt cttattactt gatgcagggc agctttttca caacctctcc
     1741 actaaacggt gttcaatcag ctaaccttcg gtattagctc aaaaaacggc aaaatatgag
     1801 gaactatttt gccgtttgtt tctctaatac tcaggtcaaa gcgctgattt cacaacctct
     1861 aatacacggt gttccagaag tagcttcttc gaaaaataag ccgaaatttc acaaaaattt
     1921 gagaagcaat tttcgtaaat tccttcttat ttcttgaagc tgaccacttc tgtcacaacc
     1981 tctaattatt aaagaacctt ttgcgaagtt aacaatcttt ctttgatcaa ttgaaaattc
     2041 tgcctaagta gtgatagatc ctgtgtgact ttcaacaata agatacttcc ctggatttca
     2101 gcaatcaacg ctttggcttg acggtctgct tcttcatcgg acagtttttc ttcagaaagt
     2161 gccaggatat tttgtttcgt atacttgacc cattgattga ctaattctag tagcaacaga
     2221 cggtattctt catccactgt actcaactca ataatcaaat tacctacagg acagccatga
     2281 taagtgatct gtgtctcatg atcagcgaaa atccagtcca acatttcttc taatgcttct
     2341 tctggctgtt ttccagcatt taagatgtct tcgaacaatt ctgtctgcca tacgtccata
     2401 tgcgcatgga tcaccgctag acaaacttct ttttttgaag aaaagtaata ataaaattga
     2461 ccttttcctg tatcagaagc tgctagaata tctcggattg acgtttcatg atagcctttt
     2521 ttataaataa gatctttggt cgcttccaag atcttttcct ttgttttcaa tgatattctc
     2581 ctttttcatt tctcatagtc ctatattata cagtaaattc atcatttttt cttcttaatc
     2641 gtttaaaaaa gactgtgcca aattatctga cacagccctc actataaaac tgttctcata
     2701 tagctagatg ttcattgatt caagaactgc gaggacaaaa gcaattccta tcttatgata
     2761 gttgctctaa tacttcctct gttgtcatca cttgatctgc aataaagcca aagttcgtca
     2821 acgctgcatc cattgcttct tggccaggag caccaattgc atctgtcgct accacaacat
     2881 tgaatccact ctctaccaat tcacgcatat gggaatcgct acacaagttt ccgttcattc
     2941 caccaagaat tactgtgtcg attccatttt ttctcaattg gagcgccaaa tcattgcttt
     3001 ctggtccaaa gattttatga ggagagctga tgatcgtatt ttcatccaaa taaggtttca
     3061 attcttcgat aaaatcggct cctgaacctt cttccactga ttcatattga ctttttctag
     3121 caaacatctg ctgctcaagc atcatatgtt ctcctgcacc gccaaattgc catttatggt
     3181 catgtgggta aaaataatga ggagaaataa agagtttgta tcctttttct tttgcttgtt
     3241 ttaccatttc aatcatattt tcgatcgtat tgtatttttc taagatatct tttgtcaaac
     3301 caaagccacc gcctgctggt ttcaagaact cgacttgcgg gtcagtgaga actactgctg
     3361 tatttttatt attgaacatg gtggattctc cattcgttat ttattttaat acatcattta
     3421 gagcttcact cattgtcgga tgagaataga tttgatctct caataacgta taaggaagtt
     3481 ttccattcat agctagcgaa atcaagttga tgacttcatg agaatcttcc gcatagatac
     3541 ttgcccctag aatcaagtca gtttcagggt cgactaaaac tttgaataca cctttaggat
     3601 cttctaatac ttgtgcttta gggacaccgc ttgtcatata cttgaataat ttgtaattga
     3661 cgccttgtgc tttggcttgt ttttctgtta aacctacatt ggataaagtc ggagtgataa
     3721 atacactgta aggcacagct ttccggttat tcgttgttcg tttattctcg cccttcaact
     3781 gatccataat gattcgataa tcatctagag agatataagt gaactgcaat ccgcctttga
     3841 cgtctccaat cgcccaaatg ttgtctgcat ctgtacgaag gtattcatcc acagcgattg
     3901 ctccgcgatc tgttagttta acatctgtat tttctaatcc aagtgatgct gtacttggtt
     3961 ttcttccagt tgcagccaaa acttttgacg cttggattgt tacttcttgg ccatcttctg
     4021 taaaggtgac ggcagctgtt gattcttggt ctgctacttg atctacagat acacctagat
     4081 ggaattcgat tcccgcattt gtcatgtctt ctaaaatcat ttgcgcgata tcttcatctt
     4141 cacgtggaag aaattcagaa tgggcatcaa gaacgacaac tttagatccg tattcattga
     4201 acattgatgc aaattccaag ccaatatatc cagccccgat gatcactaat ttttccggta
     4261 attgttcctg atccatcgct tgcgtagaat caatcaagta gcgacttgta ttcaatcctt
     4321 tgattggtaa aatgattggt tcagaaccag tattgatgaa aatccgttcg ccttcaacaa
     4381 tcactttttc accattattc gtcacttcga tttgatggtt agaaagaaaa cgtgctgtac
     4441 cgtccatcac tgtcccagtt gcctcatcgc tgatcatatg ataatttttg gcgcgaagca
     4501 tgcccgtcaa tttttctttc cgagcaacag cctctgtaaa gtcgattcct ctttctccat
     4561 tgaaaatcaa tgatttagaa ggaatacagc cgatgttgat acatgttccg ccatacatac
     4621 gtgttgactt ttcaacgact aaaaccgatt caccttttga agctaacgcc ttcgctaaag
     4681 tcttaccgcc ctttccaaaa ccgatcacaa tattttgata tttttccatc atttttccct
     4741 cgcttctttt ttacctaccg attggtacaa aaatatgata accgataatt gtctattctc
     4801 caaatcatct gctttatttg tcaaaaaaaa ccagacaaaa agcggtcctt tcacgttttt
     4861 agtctggttc gatttccttt attcttctaa acaatcgtta cttctacatc gatatttcct
     4921 ctagttgctt tggagtaagg gcagaattca tgcgcatctt ctgcaatttt ttgggcttct
     4981 tcttcgctga tgccatcgat atgcacttct aatactacac cgatctgata gccgccatca
     5041 tctaaattat aaagagagac acgtgctttt actgtggact ctgctgtcat tcctgcttct
     5101 ttgatcatat gttgtaccgc cccattgaaa caagaagcat aacctgctgc aaataattgt
     5161 tctggatttg ttccttctgc atggattcct ggaggagtga ttttcatatg catcgagcca
     5221 tttggtgcct cgacttctcc attgcgcccg ccatggttga tcattgctgt ttcgtatgct
     5281 tttttcattg tactgctcct ttcgattgac catttgctac ttggctataa ttatagtgta
     5341 tgacaagtaa ggcgctttct acaaaaattc tgcttattcg aataccagaa tgccgtcttc
     5401 tactattttt ccaaaaaaca aaaaaatact actcgtgaac aacagcgtca tatttgttcc
     5461 aaaataaata gcaatctttt ctacataatc ttttatatga ttgattttaa gatacatata
     5521 taccagaaag ttgaccccat aaaccaatac tgttaaaaaa ataaccatat tcgttaccat
     5581 tttttcctcc ttttgctggt ctccctacca gcactcgatt taaccatacc actttttttg
     5641 ccgtccaagc aactagcctt tcaaaaagac tggtttattc tgctgttaat gattcaagat
     5701 cagaaaaatg tccgaataat tttgctaatt ttactaacgc caacttgttc tttccgaatt
     5761 catcattttc tatctgtaat actagtaacg gctcatgtaa gcttaaacgg atcaaaaacc
     5821 agccgtttcc gtatggctct tctagatcat atcgaatacc ttcttcattt tccgggtcga
     5881 agttcatccc tttttcttct tccaacagtc gcttcatttc ttctaatatc ttgtttcctt
     5941 gttcgcgtgg ttcatcgctg aacaatttta atcggatttc ttgtacttct acgggctgtt
     6001 tcaactcttt gatttgttca tctaatgtga taccttttct ttttaattct ggcaaaagca
     6061 tcaggatctt tgcgatgaca tatgcaccat catccagaaa atagttttcc ttgaatgcag
     6121 cgtgtccgct tgtttcgatg gctagctgtg tatctttccc aagacggtta agttctaatg
     6181 ctttattgat tacatttcga tagccggaaa tataacgaac ttggatgcct cctttttctt
     6241 caatgaatct ttgtagatga gaagaagtag gggaattcgt cacgattctt gttcctggat
     6301 gctcttttaa tacgattgta cttaatactg cgatcaaatt atttcgattg atcactgttc
     6361 cagaagcact gacaactgca gaacgatcca catctgtatc gaaaatgatc ccaagatctg
     6421 cttcatttgc taaaacagcc tgcttgatac tttccatcgc ttctttatta tcaggatttg
     6481 ggatatgatt aggaaatcgg ccatctggtt ccaaaaattg ggaacctgtt gaatccgcac
     6541 ctaagctatt caatactttt tcagcaaaga atccaccagc accatttccg gcatcaacaa
     6601 tgattttcca accatttagt ggtttgcttt ctagtgttcc aatccccttt ttaatttttg
     6661 tgactaaatc ttgcgcataa ggtgtcaaaa ggtctgcttt ttctacgctt ccagtttgtt
     6721 cgctttttat tttttgctgc tgtgatagga taaaagcaat atcttctttt tctgctcctc
     6781 cttctgaggt aaagaatttt attccattat aataataggg aagatggcta gcagtcagca
     6841 taaccgcagc atcgcacgag aactgttcaa attgagtgga cataaacata gcaggcgtag
     6901 tagctaattc gaaatcgatg acatgtatcc cttgttcttt cagactttgg atcaacgcct
     6961 ctttcagtgc tggtccgctt atcctactgt catgaccaat cccaatcgtc cattttttat
     7021 ttggatcggt tgatttcttc tttttcttta tccagtttgc tagtccccag ccgatctttt
     7081 ttaccatgtc tgtcgtcaag tttacttcat atccttcaac tggtaatgcg attcctcgaa
     7141 tatccgatcc attttgcagc gctttcatat ctttcatata aaagtcctcc tcatttattc
     7201 cctcattttt tccagctttt ctgcaaatcg ctgtgcttct tcttctgagc ggcaaataat
     7261 aatgaatgta tctacaccgg ccatcgttcc gacaacttca gggtaagcca cttcatctag
     7321 ccaattcgtt accacatcgg caccatttcg ttcagtcaat acgataacca tgaactgaac
     7381 agactggata cgcaaaacct cgcgtctaat tgctgtacgc aaacgttctt catttaaaga
     7441 aacagatggc tggttaaata atgtatagcg tactattttg ttttcatttt gactttttac
     7501 taacttcatt tctcgaatgt ctcgagaaat cgttgcttga gtcacatcgg cgcctgcttc
     7561 atgcagccgc gcaagcagct cttcctgtgt cccaataggt tgttctgtaa tgatctggcg
     7621 gatcagtgct tgtcgctgtt tctttttcat attagacacc tctcaatgtt gattatagca
     7681 aaagataagt aatatttctt catattttgg tattttttac ttttattttt tgttttcatt
     7741 caggttgcta acctttttct attttaacac gcaaaatcgt cttcattttt tcaggtgctg
     7801 attttcttgg atcgctgatg taaatctctt tgtgttcttt agaagctctt tcatagcctt
     7861 cttttgcgat gaatgcttct aatttctcaa acgttgctgg ttcttcggca tagggcccga
     7921 cgtgtaagat ttgggcacaa agaccttctt ccatcttttt gaatactagt tgttctttca
     7981 agtcttccgg aagtttgcta gaacgctgaa tggcttcttg tgctatcgct tctgtaacaa
     8041 agtcaggctg tcggatcatc aattgataag aaaaatgttc ttttttcatc actggttcat
     8101 gcaaatactt ttcttgcagt ccccattgtc cttccagtgg atagacagta tacatctgat
     8161 aatcaggaat cagccaattt tttttaggcg acatgcgtat tgcatagctg actgcatata
     8221 gacaggctac tcttcgttta aagtcttcct gattcgggtc gccgatccct ctgatgctaa
     8281 agaatggttt tttaggaacc tctagtattt gcgggctttt tttcatacta tagctcttgt
     8341 ctttacgcca ttcaattttt tccatttttc ttcctccgcg aaattccatt tagttattta
     8401 ttatcatacc tctttttgag tgacttggct aatcgtgttg acactatttt tcagtttgtg
     8461 ctagaataag tgaagtaaat aaatcgtaca gataacacgt ttcggcgtgt cgagagagag
     8521 gcggtttcta tttttataat agaaatcact ctcttttttt acgaaatttt ttagaggtga
     8581 tgtaaatgac aaaacaaaaa cagctgcttt tagtacctgc agtaattgca acagggatga
     8641 tgtcttttgc gggcgtattg atcgagacgg ccatgaatgt aactttccca actcttatca
     8701 gacagtttga tttaacgact gcacaagtcc aatgggtaac aacgatttat cttttgatga
     8761 tttcgatcat tgttccgctg tctacttatt tgaatcgcaa ttttagtctc aaaaaactct
     8821 ttttagcttc aaatctgctt ttcttggcag gtataatgat cgatttcttc tctcctacct
     8881 tcatggtcct attgtttggt cgattgctcc aaggtgcagc gacaggtatc gctcttccgt
     8941 tgatgttcca tattatcttg acatttgccc ccctcaatcg acgaggcgcg atgatgggtg
     9001 tcggtacttt gacaacggcc attgctccag caattggccc cacttatgga ggcattatga
     9061 cttcacattt tacatggaat catatcttcc tgtttcttgt gcccgttttg attctttctt
     9121 tgattatcgg ctggtttgct attcccgaga tggcagtaca aaaaagtggt tatttggatt
     9181 ggataagcgt tttaggaatt tgtcttttgt ttagtggaag tctaacattt ttgagtacgt
     9241 tgggaacagt ttccagctgg attgctttaa tcatcggcgg tattggctgg ttcctttttt
     9301 actatcgaag taaaaaagtg gagcatccac tgatccgact gtctattctt tcaaataaaa
     9361 cattccgctt gtttttgatt ggcttcttag tttatcagtt tttattactt ggtgtatctt
     9421 ttgtcttgcc gaatttcctt cagatcgttc aaggaagtag tccttttgtc gctggattag
     9481 cgatgcttcc cggcgcagcg gttggtgctt tgctttctcc tttttccggg aaaatgctgg
     9541 atcaatatgg tccgaagaag ccaatttttg ctgggctgat tctttctttg attggttggc
     9601 tagcattatt cctactcata ggacacgctt cagtgggtgt attgattgct tgtcatgtgt
     9661 tttatatgat cggtatcgga ttatcttaca gcaatatgat gaccaccgga atgaatgcac
     9721 tgaatgaaga actacaagga gacgggaacg caatgttcaa taccttgcag cagttttctg
     9781 gcgctgtggc tacttcatta gtagcggtca ttatcaatta cgtccagcat catacttccc
     9841 ataactatga agtgtctact acattaggat caaaagcagc attaggcgtc ttgttgctgt
     9901 tgcttctcgt aagctttgct cgattcgcct attatctgtt ctttgctaaa aaagcgtaga
     9961 ttccatatac ggcaggtgtt gcatctgccg tttttttgtg tgaaaatggt tgtttgtaaa
    10021 aggaggcttt tttatggaaa aaattgccat tatcggtggc gggatcattg gtatgactct
    10081 agccagttac ttagatacat caaaattcaa tgttgcttta tttgatgaag ggacaggaca
    10141 ggcaactaaa gctagcgctg ggattatctc cccttggttg tcgaaacgca gaaacaaaca
    10201 atggtatcgt ctggctaaag acggcgctgc ttttttccct aaacttataa aagatttcca
    10261 acttgatcaa acgatttacg cacaatcagg gacacttttg cttcgacctg aaaaagattt
    10321 agctgaattg gctgttcttg ccgagaaacg aaaagaagaa gcgccagaaa ttggaaacat
    10381 cgaatggctt tcacctgaac agacatctca agcacttccg ttattgaaac cagcagtttc
    10441 actgcgtatc agtggtggtg gtcgtctgga tggaaaagca tatttagaac atcttggtca
    10501 gcgtttagca caaaaacaaa tggagcagat ccaggcaaaa gtcactttgc ataagaagag
    10561 cgattcttgg ctcatcaagg gaccgtttgg agaatacaca gctgatcgtg taattttgac
    10621 acctggcccg gcacttaagc ctttattaca agaattaggc tatgctgcag atatacgtcc
    10681 gcaaaaaggg cagctcttag catttcagac atcttataag atgagtcaag actggcctgt
    10741 tgctatgctt gacggagaag ctgatttcat tcctttcatg gatggaaaga tccttttagg
    10801 tgctacccat gaaaacgatc aaggctggga tttggctcct actagcgaag cttataaggg
    10861 attgactaag agtacagaac agttcttgac tgatccagat cagataaaaa actggccata
    10921 cgagttccgt gtaggtaccc gtgcttatac gtcagatttt tccccatttt tcggcgtact
    10981 gccagatgat ccaagcttac tggctgctag cggtctaggc tcttctggct tgaccacagg
    11041 tccttatatc ggctatctat tagccaactt cttaaatact ggtcattggt ctacggaaga
    11101 ttatcaaaag gacatctcca cctatattac gaaaaatcca tcatagaaaa acagcttttt
    11161 caagtaagga tcccgagcga tattgcgcat ggattacttc ttgaaaaagc tgttatttta
    11221 gttcaatttc tgaatcaaat tctccaaact ttcttttgct tcatctggca agagatcttt
    11281 tcctatctga gcttcaatga atcgtaaacg aatatcaagt cgttgtttca ccagattttt
    11341 ataggacgta tcctcaaaag aaacaggaaa atctggtata ggtaaatagt ggatgtcttc
    11401 aaatggacca aacgacttga attctgccgt ttgttgcacg attctttcaa cgattgacaa
    11461 tgtcgtttca ggtttgattt ttttatcatc gaaatatcca gtattcagga catagcaggc
    11521 tgtttgctgt ttcaaaaaca actctcgaaa agcttgatag tcttccctaa gaggatagac
    11581 acggaaagga ttagcaaatg gttcaatgac tagctcatct agctgtcttt ttgtagtgat
    11641 attttccgcg tctgatcgtt ttgttgctaa tgttgcacca aaagacgctg ctaaaactgg
    11701 gtcattgatc ttgataattg gcggaagcga atcatctttc atgatccaaa aaacagcatc
    11761 gatcttttcg ctaagatgat cgactcggtc gggtgtcgca taacgtgatt tgactgtccg
    11821 cccatttccg tttcgtatat ctcctgttac taggactttt tctcctttac ttgtacatgt
    11881 cacgcccaca ttctgacaag taaggaaaga agaaatggcc ggatcatcag acggatagtc
    11941 ttgtgtttta tcaaagtaag aaggttccaa ggcagtagca aaaccatttt ttcgagaaat
    12001 aacgaaagca tcgtcatgca agatcgaaac agagtgacct tccgtatgtt ttgctaaggt
    12061 aatcgttgat tttccagaac cggacaaccc aaatacagcc atcgtatagg tctggtcatt
    12121 actagcatat tgtttcatcc caccgtggca ggcaataaag ccattgcgat ttcccaatgc
    12181 ccaagcaagc gtcaaagtcg ctttttttaa ttctccaaag taacgtaatc ctagcacagc
    12241 cgctgtatta tggagagcat caaacaaaat gagtccattt ggaaatgttt catgtgtcca
    12301 ttcgggatca caaaagatat aaagatctcc ctcatcaaag gaaacagact tttgataagt
    12361 ctctaagatc ttcgaattcg ctggctgaaa attcagcaaa taagaataca aattgttctc
    12421 aaatccttct ggaatcagca aatgactttg cacagaaaat tcttcagata ggccaacgat
    12481 tacttttgta tggtaaaact ggcgcttgcg actttgatac acagcttctc ttaaaatttt
    12541 ctcgtatttg ctttgttcct tgtctaagtc ctcaaagatc cgccgagcac ttgctgttct
    12601 tcccacaatg cttccatcat tgaatactag tgtcctaaca tcagctggta aatctagttc
    12661 ttcagcatgt gagattgtta aatctgtgat gatcgttcct ggactccctt ttgctagttg
    12721 ataagctgtc ttcctgtcaa aaacttcatg aacattatta ccgtaaaatg tcgtttctac
    12781 aatcgatcga atactggata acagtggatt tccacttgtg atttcgttta attcgagact
    12841 ctcttttgta ctcatggctc ctcctcattc cctaaacaaa aaaacgtttt ctttattata
    12901 cattattcga gtttttattt gtactgctgc gctgtgggaa gcaaacgagt tatttttcac
    12961 a
//
LOCUS       DACDXO010000074        12734 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E23605_2017
            SAMN12639989-rid9881463.denovo.075, whole genome shotgun sequence.
ACCESSION   DACDXO010000074 DACDXO010000000
VERSION     DACDXO010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639989
            Sequence Read Archive: SRR10023389
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741914.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:42:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,972
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 33 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 17 of 73
            Pseudo Genes (multiple problems)  :: 10 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12734
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639989-rid9881463.denovo.075"
                     /isolate="E23605_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(85..264)
                     /gene="rpmF"
                     /locus_tag="IWM35_002343"
     CDS             complement(85..264)
                     /gene="rpmF"
                     /locus_tag="IWM35_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HAP9920171.1"
                     /translation="MAVLARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(322..891)
                     /locus_tag="IWM35_002344"
     CDS             complement(322..891)
                     /locus_tag="IWM35_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HAP9920172.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            1077..1979
                     /locus_tag="IWM35_002345"
     CDS             1077..1979
                     /locus_tag="IWM35_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP9920173.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            complement(2014..3798)
                     /gene="pyk"
                     /locus_tag="IWM35_002346"
     CDS             complement(2014..3798)
                     /gene="pyk"
                     /locus_tag="IWM35_002346"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HAP9920174.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(3872..4834)
                     /gene="pfkA"
                     /locus_tag="IWM35_002347"
     CDS             complement(3872..4834)
                     /gene="pfkA"
                     /locus_tag="IWM35_002347"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HAP9920175.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(5002..8316)
                     /gene="dnaE"
                     /locus_tag="IWM35_002348"
     CDS             complement(5002..8316)
                     /gene="dnaE"
                     /locus_tag="IWM35_002348"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HAP9920176.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSI
                     KGQLIYLLGEDNVVFK"
     gene            8547..8732
                     /locus_tag="IWM35_002349"
     CDS             8547..8732
                     /locus_tag="IWM35_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HAP9920177.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            complement(8794..9990)
                     /locus_tag="IWM35_002350"
     CDS             complement(8794..9990)
                     /locus_tag="IWM35_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAP9920178.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVARAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(10160..11596)
                     /locus_tag="IWM35_002351"
     CDS             complement(10160..11596)
                     /locus_tag="IWM35_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAP9920179.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
     gene            11889..12698
                     /locus_tag="IWM35_002352"
     CDS             11889..12698
                     /locus_tag="IWM35_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP9920180.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
BASE COUNT         3803 a         2674 c         2249 g         4008 t
ORIGIN      
        1 attttttatt taccgactac aaaaaaataa acctcaaggc tccatggtta gccttgaggt
       61 tttacatttt ctaattaaaa tgtattaagc ttcttttgac attacgtcct taccatcata
      121 gtaaccacaa tttgcacata cgtgatggct ttttttcatt tcaccacagt ttggacattc
      181 gtttaaccct ttgattgtca atttgtagtg tgtacgacgt tttgcttttt tagcttttga
      241 tgttcttcta gctagtactg ccattttgct acacctcctt gtaaagtctt gtatctaaat
      301 tagacacaat atattccaat cttacttgtt atcgtcgtct tttgatttag aatcaaaaag
      361 ttcagatagt tttgcaagac gaggatcaat tttattctcg gcttcttcca tttttctgcg
      421 ctggtattct tcttcggaaa caacttccca atcattgcct tttggaagtt ctgttgctgc
      481 ttcctcttct tttgtcaata cccgcattgg aatcgccaat aagatgttgt cttcaatcga
      541 ctctgctaaa ttcaataatg gaccttcaat tagcaaaatc tcatctgcag acatttgttc
      601 atctctactt tggaattgtt cttctgtcat gaatacttcg tctacagaaa aatccatggg
      661 cagttcaaca ggttctagtg aacgtgttga aggaactgtc aaagtcgtct gcacagtgta
      721 atgaacgata tagcctttct tatcaactga gaccaaacct tcgacttcca ctggcgcaac
      781 atcaagtatt aaattgtctc gcttcatgag agcggctttc aagtcgagcg tctcatgaaa
      841 agttaacggt gtctcctggt attttctcaa ttccaataaa gaccatttca tttttcatca
      901 ctcctaagca acaaaagcaa ttatacaggg caaaaagcat gttgtcaatg aaattttctt
      961 gacaccttgc agttcataaa aaaacagaaa aatctttatt agaaagcctc tgtttgctat
     1021 catcctcaga atcatttaca ataatcatta ttccattaaa ggagcggatg atcttcatga
     1081 acacgaaaga tatcgaatac tatgtccaaa tcgtcaaaca aaaaaatttt acgaaagtgg
     1141 ctaattattt ccgtgtgagc caacctacta ttacatatgc attacaacgt cttgaaaaag
     1201 agtttgacac cgtacttatt acaagagatc gatcgcatca cgaattaatc gtcacgcctt
     1261 ctggaaagca gctgtttctc catgcacaaa ccattctcca agagatttct actgcaagag
     1321 ctgaaatcac tcagttgcaa aagaaaaagc ttcgttttgg catgcctcct attatcggca
     1381 atgaatactt tcctaaactg tcaagttact ttatcaagca tgaattgatg aatcaaattg
     1441 agattaccga tggaggctct cgggacttat acgggatgat ccgacaaggg aggatcgatt
     1501 tagctgtatt aggctcaaca cagccaatcg tggatcaata tttagaaaca gagttacttt
     1561 tggacaaacg tttcatgatc gtcgtgccac ctacacatcc tctttccaag caggatactg
     1621 tctctttcaa agatatagca gatgaacagt ttgtactatt gaacgaacat tatgtccacc
     1681 ccgcagcatt cactaagctt tctaaacaaa cacatactca tccaaaagtt gtttatcaaa
     1741 gcaatgactt gacgatttta aagagtatgg tccgagaaaa tgtgggtatc ggttttttgg
     1801 cagagattgc tgtccatcct gaagatcatt tagtttgcct ccctctttca gatgaagttc
     1861 agcctcgctt cttgatttca ttggcaaaac gaacacagca gatcgtcacg cctcttcaag
     1921 aacaagtagt ccaagttatc catgaattcg ttacagcaca aaaaaacagc tcttcgtaaa
     1981 agaagagctg tttttatttt ttattaagct aaattagata gctgttgttt cgccacggta
     2041 aacgatgccg cgacgtgaat caacggtaat caattcacct tctgtaattg ttgttgttgc
     2101 gcctttagca ccaacgatta caggaatatc ttgtgcaatt cctacaactg cagcgtgaga
     2161 agtcaatccg ccttcttcaa cgataacagc agctgctttt tcgattgcag gcatatattc
     2221 tttgtcagtt gatggtacaa ctagaaccat tccgtctttt gcttttttta cagcttcttc
     2281 agcagatgaa gcaataactg catttgcaac gacagatttt tgtccaacgc cttgagcttc
     2341 taataattta gaaccaatca tttgaatttt catcacgtta gttgttccgc gttcaccaac
     2401 tggtacacca gcagtgatca agattaaatc gccttctgat gcgaaaccta attcaacagc
     2461 ttttttagct gctagttcaa acatttcatc agttgttgat ggtttttcag caactgttgg
     2521 gtaaacgccc cagtttaaca tcaaaccacg ttttgtgcgt tcgtcaaaag taacagccaa
     2581 gatatctgca tctggacgat attttgaaat catttttgca gtgtatcctg attcagttgc
     2641 agcaacgatt gttttcacgc ctagattttt tgcagcacga gctacagata aaccgattgt
     2701 ttcagttaca tctgttttat cgaattcgtt caattggaat gtacctaatt ctgataatgc
     2761 attttcagct tcttggtcga tacgtgccat tgttgaaaca gcttcaactg ggtagtcccc
     2821 gtttgcagat tcaccagata gcattgttgc atctgtgcca tcaaatacag cgttagcaac
     2881 gtcagaagct tccgcacgtg ttggacgtgg gttttcttgc attgattcta acatttgtgt
     2941 agcagtgata actgggatac cagcagcatt acattttttg ataatgcgtt tttgaaccat
     3001 tggtactagt tcagctggga tttcaacacc catatctccg cgggcaatca taacaccgtc
     3061 agaaactttg atgatttcgt caatgttgtc gataccttct tgagattcga ttttagggaa
     3121 gatttgaaca tgagtcatgt ctttttcttc taagatttcg cggatatcta gaacgtcttg
     3181 tgctttacgt acaaaacttg cagcgataaa gtcgatatcg ttgtctaaac caaaacgaat
     3241 atcgtcagca tcttttggag taatacctgg caaactaata gaaacgcctg gtgcatttac
     3301 ccctttacgt gatcctagca tacctgcatt tttaacttct acgactaatt cacggtttgc
     3361 ttcgtctttt tcaatgattt gcatatcgat caaaccatca tcaaataaaa tgtgtccgcc
     3421 aacgtgaaca tcgtcaaata atcctgggta agtaaccgcg attttttctt ttgaacctac
     3481 atgttcagga tccatagaga tgcgtgtttg atctcctact tcaaatttga tgtatcctgc
     3541 acgaccaaaa tcagcttctg ttgtgccttg aacagttgta cggatttcag ctccttttgt
     3601 atctaaaaga ataccgacat cttttcctgt ctttttaact gcttcacgga ccatagtcat
     3661 acgtgataat tgttcttcat ggtcaccatg agaaaaatta aagcggaaga catttgcccc
     3721 agattcaatc aattgagaaa taatttctac tgagttactt gctggtccta atgtactaac
     3781 gattttggtt tttttcattt aaaaaactct cccattcaat tttgtttatt taaagctgta
     3841 cacagcttaa atagcttggt ttctttcact cttaaaatga aagtgaatta tttagatcat
     3901 atagtgataa gtctggttta tgtttgttgt tttcaagtgt atcaatgatg tcagacgcaa
     3961 cgatttcgtt gtcatggata ccgacacata atccaccttt tccttcttga agcaattcga
     4021 cagcgtaagc accgaattta cttgccaaca cgcggtcgcg tgcgcttggt gctccaccac
     4081 ggacaacgtg tcccaagata gatacacgtg tatgatagtc accaaattct gaaagtttct
     4141 cagcgaattc atttccgccc attacacctt cagccaagat gatcaagcaa tgtttcttcc
     4201 cgcgatcgcg gccttcttgg atttttttag caactgaagc catatcaaaa tcatgttctg
     4261 ggatgatgat ttcatccgct ccgccagcca cacctgacca taaagcgatg tctcctgcat
     4321 tgcgtcccat tacttcgata acgaatgtac gaacatgaga agtcgctgtg tcacgaatac
     4381 ggtcaattga ttctagtact gtattgattg ctgtatcaaa accgattgtg aaatcagtcc
     4441 caggaatatc attatcgatt gttccaggaa tacctactgc tgggaaaccg cgttttgtca
     4501 aagccattgc gccatgataa gagccgtctc ctccgataac cactagacct tcgattccga
     4561 attttttcaa ttgttcgatt ccttttaatt gaccttcttc agttgcaaat tctggataac
     4621 gtgcagaata caagaatgta ccaccacgtt ggatcttgtc acctacatct gccacatcaa
     4681 gacgacgaat atctccagca actaaaccgg caaaaccata attgatcccg tatacttcca
     4741 gtccttcgta aataccttta cgaacaacgg cacgaacagc tgcattcatt ccaggtgcgt
     4801 ctccgccact ggtcaaaatt ccgatgcgtt tcataataaa cattcacctc attaaaatac
     4861 tttatcctaa gtatgactta ggtttacaac catttttgcg ttaaccacga ctaacattct
     4921 aacattattt ttctcacttg tcttgattta cggataaaaa aatgaaaaaa aatgaaagca
     4981 agtagtattt tttaccgtaa gttatttgaa aaccacatta tcttcgccaa gcagataaat
     5041 aagttgcccc tttatactct tgctgtcatc cacccaataa gcatctgaca gaactacttt
     5101 tcgttgcgtt ttttcgtgat acataactac tggtatatat cctgggtgtt tctgaaaaat
     5161 cacttgtaat tgtttcatta tctcgggatc ttcccgttct tctgtaatac gcaaatagca
     5221 agtttgtgaa ggatactggg cttctaactg gttaggatca gcaaagcttt ctccaattag
     5281 ttgcagttcc tgattatatt tgctgatttc gctccgccct tgaacaaaga tcactcgatc
     5341 aacggcaaac aaattacgat atttacgata aagagttgga aaaactgtga cagaaatttc
     5401 tccactactg tcggttcctt caacaaaggc catcagttct cctttttttg tccgaatttc
     5461 tctaacttcc ttgatcatca ctaataaatt gactggttga ttagggacga tatctgtaat
     5521 cagctgaact tgtttagcta aacgaagacg atcaaaccct tctgcaggat gacctgacaa
     5581 ataaatacct aagtactcct cttccagctt caagcgttct tctagtgtat agtcagcgac
     5641 ttcttcttct tttaatgcca tgattcccag caagtcaaga cttccaccac tgtaaacgat
     5701 attttggatt tttccttcga gatcaagcat caattgtttc ctgttaggag ataattcatc
     5761 aaaaactcct actgctgtca gtgattgcag taactctaat ttcagccaac gcttatcgat
     5821 ccgtatgagg aattgatcca ctgactggaa cgggccattt tgttttcttt cgtttaatat
     5881 atcttctaca aaatcacgcc gaataccttt tatcgctgtc agtccgaatc tgatttcttc
     5941 cttattgacc aactcaaaac tggaattact ttgattaatg gacggcgagg aaagtttgag
     6001 tttattttta cgtgcttctg caatatattc tttcaatttt gatgtgttat gccgaacaga
     6061 attcatcagg ctggtaaaga aagcacctgg atgatgggct tttagatacg ccatttggaa
     6121 tcccacgaat gaataagcaa atgcatgtga ccgattgaat ccatagtttg caaatttttc
     6181 gatatattca tatacggtcg ctgctgtttt ttccgaatat ccctgttgta atgctcctga
     6241 tacaaaatgc gacttttctt catctaatac gtcttttttc tttttactca ctgcccgtcg
     6301 cagaatatct gactgcccta atgtaaatcc agccatagtc gaggcgatct gcatgatttg
     6361 ttcctgatag acgatcactc catatgtgtt tttcaaaatc gggatcagtg aatcatctgg
     6421 gtagcgaatg gcttcttttc ctttcttcct ggcaatgaag gtatctatat tttgcatagg
     6481 gcctggtcga tacaatgcat tgactgcggc aatatcttcg atgttttctg gatctaatct
     6541 tcgtaaaacg ttccggatac cagcagattc aaattgaaat actcctgagg tttcgcctcg
     6601 ttgaaacaag agcagcgttt ttgcatcatt tagcgggatc tcttttaatc ggatctcata
     6661 cccttctact cgcttaacag ctttcaaagt aaaatcaata atcgataagt tgcgtagtcc
     6721 taaaaagtcc attttcaaaa gaccgattgc ttctacctca ttcatcgtat actgggtcag
     6781 aaaagcttca tcagaaccgc gttggagcgg aactagttca agtagattct tatcgctgat
     6841 aactacacct gctgcgtgag tcgaaacatg tcttggcaat ccttctaaaa tcactgcagt
     6901 tttgaataaa agttggtttt tttcgcttgc ctgaactaat tctactaatt gatgcgaagt
     6961 tttataagct tgggtcaatg tgattttcaa ttgattgggt attgcttttg accagcggtt
     7021 tgcttcactt tgcgataaac caaacactct agctgcatct ctaagaacca tttttgctgc
     7081 cattgtgcca aatgttgcga tttgtgccat atgatattgt ccatactttt ctcgaacata
     7141 attcaatact tcttcacgtc gattgtcagg aatatccaaa tcgatatctg gcatcgtaaa
     7201 gcgttcgggg tttaaaaagc gttcgaacag caaatcatat tctaatggat ccacatctgt
     7261 gatttctaac acataggcta ctaatgaacc cgcagcagaa ccacgacctg caccggtaac
     7321 gattttgttt ctatgagcaa aagccattac atcccatacg atcaagaaat aatcatcgaa
     7381 tcccatttga tggatgacag ataactcgta gtccaaacgt tcttggtatt cctttgtata
     7441 gtcagtgatt cggcgttcca cttgcgaata gcataactct cttaaatatt ctccagcttg
     7501 ttttccatta ggaactggat aatgcggaag taatttctga tggaactgga attcaaatga
     7561 aatcgattca gctaaacgct gggcgcctat tacagcttgg gaaagacccg ctttttcgaa
     7621 tcgtttgatc atttcttgtt ctgaaagaag atagacattt gctgttagtg gtaaagatgc
     7681 agataaattt tctaaaggta tttggcttcc ctctttgata tgtttcatca cttcaagtgc
     7741 aaaaagttct tcttgttgta aactagcgat gggatgtaat gcacaaagcg aataattttc
     7801 ttttatcaca tactgatacc attcaggcaa ttgatcaggc gtgttttctg tcaagccgat
     7861 ataaaaattt ctagtaaaaa cattttcaag taaaaactgc caatgaccga aaaagttatc
     7921 ctctttattg aaagcctcag aaacagaatg atttgccgga acaacaaacg ctagatggtc
     7981 ggtttctgtc atttctgcaa gttccaattt ttcttggatc atttttcggc tggaaagttt
     8041 catcagctgc tgataaccta tttcatcttt tgcgtacgca agcaagccat aatttttttc
     8101 ttcataagta taactaagat gtaatccaat gattggctgt actccttctt ctacacaaat
     8161 acgatagaat tcaggtgcac cgtataagac atctttatcc gttatggcta atgaagtgta
     8221 gccttgtttt tttgcttctt gtacatattc tcgaatacgt atcgtacttt gtaaaagaga
     8281 ataagaagta attgtataaa gttgtgggaa aaacatcttg cattccgcct tttttttcgt
     8341 taaaattcac tagcgttaaa aggtttgatc cttttacttt cattaagata gcaggcgctg
     8401 aagttgatta ttccacttca tttgcttgct tgtaatctgt aggaatgagt tctttcgcaa
     8461 gaactcaaat tttattgaga tttggtctcg tttctttaca ttcaaagaaa atctggtaaa
     8521 attaagatag aaaagaggtg ttctttatgc gatatatcgt tacattattc tgggctgtcg
     8581 ttctaggtca agtagtcggt tatattggtg ctgcattaac aagcggaaca tatgatttta
     8641 ctttgacaac aatcatttca ttcattgcgg gtgtcattat ccttttgatt ggtgcagttg
     8701 cgccaagaaa agaaacctct gcccattcgt aaaaaaacag ccaagagctt gagatttctc
     8761 aagctctttt ttattttcta ctcctcacca ccattataat ctgctgcgaa gaactttacg
     8821 aaagttgaat aaagaaagaa gaatatttga cagaacaaac aaacttgtca caataaaaac
     8881 agcactgtaa ttataagcac gcgcgactgt cgatcctagc atgggaccta aaacttgtcc
     8941 aaagttgcta aacatctgat tgtagctata gattcggctg actccttctt gaggcgtaat
     9001 cttactgatc agtgtattga cagaaggcat caaagcgcca gttgaaaagc ctaaaataaa
     9061 gcgaagtata cctaattgga gcggtgtagt gacgaatgcc ataggtaaat aacagcaaaa
     9121 tgataaaatg agtcctccaa ttagaacttt ttgattccca atcttatccc ctagttttcc
     9181 tagtttcggt gaagaaaaga cagcggaaac accagctatc gaaacaatca agccgctgac
     9241 aaaaaggata ttttctgtac ttccgctcaa ttcacgaata tataaggtca aaatagggct
     9301 gatcgtagtg attcctatct gtaaaatcaa ggtcgtaatg aagagaccga tcaaaataga
     9361 gagatgatcc atttttgaaa aaatttcttt ggtgcttatc agatcttttt tctcgattgg
     9421 atggaaatct tctttgacta agaaaatggt caataccgtt gtgatcatca acaaagcccc
     9481 tgtgatcaag aatacatttt ccataccaaa ccactgagcc agtgcaccgc ccattgaagg
     9541 tccgatcaga tttccagcaa tcgctccggt agacaatgtt cctaacgccc atccgctctt
     9601 ttctctaggt gcctgtgaag caatcatcgc tgtggcattt ggaatatatc cagacaaaat
     9661 cccattccaa aaccgcatga tcaaaagcca atacgcgttt ggtacaaaag ccaaagatcc
     9721 cattgttaat gtcatccctg ctgctgcacg gatcatcatg agttttcggc cttttcgatc
     9781 agctagattt ccccagattg gtgcaaccac ggcagatgca aaagcagtca cagagattgc
     9841 tagtcccgaa aacaattcga tttgatcttt tggtgttcct agttgctcaa cataaactgg
     9901 tatgaatggc ataactaaac taatacttgc accagtgaaa aaacagccaa tccacgcaat
     9961 cactaaattt tttctccaat taatctccat ctatcatccc tctttcgtct cttttatcat
    10021 acctattttc gaataaacaa gcaacgatct cgccagtaaa aagagaaaaa tcccgcaaag
    10081 gtcattttat atgtaacgtt ctttttatgt aaaacaaaaa gagatcagaa caaatactgt
    10141 tccaatccct tctctttagt taaattgttg ctagaatgat gaagttgata atgaataaaa
    10201 tatcaacgat ccacaaagca acagaaactt gattgtattt tcctttgatt actttcacaa
    10261 ttgtatagaa aataaatcct gctgcaatcc cataggaaat gctatagcac aatcccataa
    10321 agatcgatgc aaagaaggcc ggtaaagctt cttccatatc tagccatttg atgtctgcaa
    10381 aagaagccat catcattact ccaactaaaa tcaatgctgg cgcagttgct tgatttggta
    10441 caatggcgat cagtggagag aataaactac ttaatgcgaa tagcactgcc actactactg
    10501 aagtcaaacc agtacgtcca ccggccccga tcccagctgc agattcgaca taagttgttg
    10561 tattcgatgt accaaagata gcccccacag aagtagcgat cgcatcggca aatagtgctt
    10621 tatccatttt tgttttgaac ccacgaccat cttccaacgc gatctcgtct tcttttgaga
    10681 aaatacctgt gcggcgtcct gtaccgataa atgttccgat cgtatcgaaa gtatcagata
    10741 aactgaatgc gatgatcgtc atcaatactt gaggaatctt ggaagaatca ctgaataaag
    10801 attgcatgcc ttcagaacca aatgctgcac cgaaagttgt tcctaattct ttaaatgaat
    10861 tacctaacga attcgtttgc caatcaatgg aacttaaatc aacaacaccc ataaaaatac
    10921 caaggactgt ggtcgctacg ataccgataa gaatagcccc acggacattt tttacaacta
    10981 aaatggctgt cagtaccaaa ccaatcacag ccaataagat aggagcgttg tcaaagttgg
    11041 ctaacgcagg gacgattcca ccatttatcg taacatttgt cgcttttccg ccttcaacga
    11101 cagagctcga aatagctgac tggtctgctg aaaaagataa gaatcctgca tttttgattc
    11161 cgacatacgc aacgaaaata ccgatcccgc caccaatcgc atgttgaaga ctttcgggaa
    11221 tcgcccgaat aatcattttt cgaatatttg tgacagtaat aaaaatattg attaatccgc
    11281 aaataaacac cattgccaag gcttgttgcc aagaataacc aagcccaaaa acgaccgtaa
    11341 aagtgaaaaa tgcgttcaat cccataccag gtgcctgagc atatggtaca tttgcgaaca
    11401 atcccattac cagcgtacca ataatcgatg caatgattgt cgctagaaat actgcttgga
    11461 agggcatacc tgatgctgac aaaatcgtcg gatttacgaa taagatataa ctcatggcaa
    11521 agaatgtcgt tactcccgcc attatttcag ttgatacatt tgtattgttt ttctttaaac
    11581 cgaaaaactt ttccataaaa agttccactc cctgaatttt tttctgatgg aaaactataa
    11641 taataaaaaa taagtaaaat agggcaaatc catcgaacaa tttcaaatta tatagacgtt
    11701 tcattcgctt ttcaacgttt ttttaacaaa aaaagcgaaa ataatactta acgttcgtgt
    11761 ttagcttatg tttagctatt tatatagcca taactgtagt ttttgttagg aatagctgat
    11821 tgctcttgtt aattacctaa aatatgctag actaaaacaa atgctagttg aggaaggaag
    11881 gacccttatt gaataaaaaa ttaattgcca tcgatctgga cggaactacc ctcaatcaag
    11941 attcgatgat tacatctaaa acaaaagaga ctctaaaaaa agcaatcaat gccggtcatc
    12001 atgtcagtat tgctacagga cgcccattcc gaatgagcca tcagttttac caacaattag
    12061 aattaacaac accgatggtt aattttaacg gcgcattagt acacatccct aatcagcact
    12121 gggatggtga aagagaaacg ttgatcaatc gtgaaatcgc ttttgaaatc ttatcgcaaa
    12181 aaaagcagct aaatttagat tttatcgctg ctgaaaatcg tgatactttt tttattgaca
    12241 gttttgattt ctttgatgaa aaaatttttg cttcttctcg tccaggagag aagaatttgc
    12301 tttctcctaa aaatttgacg acaaatccta catctttgct tgtccgaaca gacaaacgtt
    12361 ttgcaggagc tgtatctgct gaattaacac gtcaatttgg cagttatgtc gatgttcgga
    12421 catggggtgg acctactgcc atcttagaga ttgtttcaaa aggaatccaa aaagcaaaag
    12481 gcgtgcaaga gattgctaac tacctgtcta tcgatcaaaa agacgtcatt gcctttggtg
    12541 acgagcataa tgatttggaa ttgttggatt atgcaggttg gggagtagct atggctaacg
    12601 gtactgacca actcaaaggc attgcaaatg atgtcactcc tttgagcaat caggaagacg
    12661 gtttagctgt ttatctggaa aaactactta aactataata gagaattaga ttccacaaaa
    12721 aagaggttgt gaca
//
LOCUS       DACDXO010000075        11668 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E23605_2017
            SAMN12639989-rid9881463.denovo.076, whole genome shotgun sequence.
ACCESSION   DACDXO010000075 DACDXO010000000
VERSION     DACDXO010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639989
            Sequence Read Archive: SRR10023389
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11668)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741914.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:42:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,972
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 33 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 17 of 73
            Pseudo Genes (multiple problems)  :: 10 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11668
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639989-rid9881463.denovo.076"
                     /isolate="E23605_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(37..216)
                     /locus_tag="IWM35_002353"
     CDS             complement(37..216)
                     /locus_tag="IWM35_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9920181.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESRIMCIALDLL
                     IAQEKAGFEVPALKK"
     gene            complement(597..1304)
                     /locus_tag="IWM35_002354"
     CDS             complement(597..1304)
                     /locus_tag="IWM35_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9920182.1"
                     /translation="MNKVVYQLTTEDVSQFLTNKEHLNEVIRRIQKDFSCMEEMKYFI
                     QRVEEQLYMESETSQTKVTVSHNRLDVYKRRPDTLKKGAELLRLETEKYIMNVYLERS
                     ELVENFVSYTICRKHYYSESEYYSIFESMELMEEEMEELNSKDATVDDYYLVFTRFLT
                     CGTESIVLMGMDYELTVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNE
                     LDRKRMPYQRFERKKGQ"
     gene            complement(1297..1734)
                     /locus_tag="IWM35_002355"
     CDS             complement(1297..1734)
                     /locus_tag="IWM35_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9920183.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYHETRVPKKHYKEKLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNGKWFFQALLCLEVGVKPSTIKPSEYQALE
                     LTYAKFAQIKKAKAMASDWLDTFENIEKNGAFYTIESEEKDNE"
     gene            complement(1749..2000)
                     /locus_tag="IWM35_002356"
     CDS             complement(1749..2000)
                     /locus_tag="IWM35_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9920184.1"
                     /translation="MKTQHKLLTVKEVVQILVDKEVISEKVAEALLIPRYWTIETPLV
                     EDAPTMLFEDLVKIAGYELHYQENMETYEIEYWLTKLEN"
     gene            complement(2000..2206)
                     /locus_tag="IWM35_002357"
     CDS             complement(2000..2206)
                     /locus_tag="IWM35_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HAP9920185.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGERAKCIKSYRLYQC
                     PTCGKKYTLSYGDYVLIDEKVKKR"
     gene            complement(2643..2903)
                     /locus_tag="IWM35_002358"
     CDS             complement(2643..2903)
                     /locus_tag="IWM35_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9920186.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIESMQIRNPDHFTRSMGI"
     gene            2978..3181
                     /locus_tag="IWM35_002359"
     CDS             2978..3181
                     /locus_tag="IWM35_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
                     /protein_id="HAP9920187.1"
                     /translation="MIKTVKFVTVAIRLKVRWCVCAYFQNLTERRSLLPVETALGLMI
                     SFGSLIATIIFGILAVVNNNQKK"
     gene            complement(3322..3807)
                     /gene="ssb"
                     /locus_tag="IWM35_002360"
     CDS             complement(3322..3807)
                     /gene="ssb"
                     /locus_tag="IWM35_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAP9920188.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQNGNR
                     EADFINCVIWRKPAETMATLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRAHADQSSSSPSPTTFEQRDVATPNNNGLNASQNPFGGQSIDISDDDLP
                     F"
     gene            complement(3810..4709)
                     /locus_tag="IWM35_002361"
     CDS             complement(3810..4709)
                     /locus_tag="IWM35_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HAP9920189.1"
                     /translation="MTPTQFAAKTRNLAKSTGLNAQLVQRHFMMDRLIEQISESKYKN
                     DFILKGGFLLGSKYGIDRRTTIDIDTTFRNSKLTEEKLKSIFNDLTRKPTKEGIVFSI
                     QGMKETREADFYPGFQIKLVANLEKVRVPFKMDVTTGDSIYPEVDTHYHQLMFEDKKV
                     EIPAYPTEQIIAEKLSATFSFGTDNSRAKDYYDLFTIPKLENIDTKELYKSVRNTFTK
                     RGDTKPLSFYYEREMDKIRGNDYLEKQWEKYRSVNTFANSISFTDTVNEIDHLMKSVI
                     KIENQERKRQFKRSQQMNQEMER"
     gene            complement(4722..5315)
                     /locus_tag="IWM35_002362"
     CDS             complement(4722..5315)
                     /locus_tag="IWM35_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="abortive infection protein"
                     /protein_id="HAP9920190.1"
                     /translation="MQDKLKELLAIRDGNLSMKEARKFGIAATTVQRLVNQGKLIKVD
                     RGYYVEDGHGIDDLFWLQQRFSRGIFSHETTLDIYQLSTNMPKFIHLTFPHGYNVDRS
                     ITKEQQLQFHFVKKEVYELGKVEGTSFQGNPITMYDKERTLCDIWNPRYEFEYEMKLE
                     ALKEYMEDPLRDPSKLRDYMTKLHVEKEMKYHMQSLY"
     gene            complement(5556..7760)
                     /gene="topB"
                     /locus_tag="IWM35_002363"
     CDS             complement(5556..7760)
                     /gene="topB"
                     /locus_tag="IWM35_002363"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAP9920191.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFSGETVIT
                     YGFGHLVELDSPDMYDENWKQWSLEHLPIFPTHYHYHVPKDKKKQFKVVKQQLQSADT
                     IIIATDSDREGELIAWTIIQQAGADHGKIFKRLWINSLEKEAIYQGFQQLRDAEETYP
                     KFEEAQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FQKEPYFEGKAQVIAQNGAFDAKLDPNETQATQEAFEDYLKEKGVQLGKQPGTIHQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLKAEAIPTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTAAAFEQ
                     LSPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKTVGKVPVDLGWKKLFGK
                     ESDRKEKEEEPLLPKVTKGEAVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGILLCQAVEKEPLLT
                     SAEMTAKWESYLLKIGEQKGTQATFLANIQKFVSHLLEVVPGQIQSTDFGSTLQEVKA
                     ASEKQEATRHLGVCPKCREQEVLLYQNVAACTSEACDFKLWTTIAKKKLTATQLKEII
                     QNGRTSQSVRGLKGQKGSFEATIVLKEDFTTGFEFSEKKKTNYKKRTRRTTK"
     gene            complement(7771..9231)
                     /locus_tag="IWM35_002364"
     CDS             complement(7771..9231)
                     /locus_tag="IWM35_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HAP9920192.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDPKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYAEKEGLIAELKPKHERQNF
                     LVRDDRLDHAVAFLWKDPQTNETVGASYQGTFIDYERFGERGTYKHIDKNSTANHGFN
                     LKIGDPKQLKFFESSIDLLSYAALNRDQLNDTWLVSMEGLKHHVISHYFGEAVSELRK
                     KQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDPFTNQKVRCERGIANDWQVPKEYKVI
                     YEEVAKEEKITPEAIMAIHKTENNLQLTNQLVSAHKVNASFGQQLSVNDSIEAINLKD
                     ICREVAKELKACERVDGTYDFDRFYQEKGDINAQILFSYKAEQYYKGYKNHEHEFVPE
                     VKKDWNDQLKHEIYQQEIRKQKRAMLFQQGRQQERE"
     gene            complement(9292..11664)
                     /locus_tag="IWM35_002365"
     CDS             complement(9292..11664)
                     /locus_tag="IWM35_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9920193.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNEQIEKMFEHQRKTNGQ
                     PMNDKRNGHSWFRQQERLENKVHSLREEIKQQEKQVEKLERQEELKEMGYNKYGGLDM
                     TIENIPRIKEEIERFEKGESTFSAATIRKYQRKLETLEQLKERSEKGKENLLPEVQAI
                     IDSGRVTQWKKNPTIYFLKDYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     APTDSLKEKENDHFYRVEFNENDPEKGLKSYGGEIVTLELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNQPIYLELAKLCEQVPEKEQKQTVKNEPVSKE
                     QMQSKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSPEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VRETEATPEDKKRIESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQL
                     DKKGQSELQAGIQAVANNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNT
                     RNTPTQHLATSIHELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTSEKAIPYI
                     AAWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQE
                     HKNTVETAEQKNIVPVGSLWIDKKDGKVMEEDTGRTLHLKDAAFIEDPQHNQVILKGK
                     SYGKEIQTALPSEEFALGNSFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
BASE COUNT         2892 a         2433 c         1957 g         4386 t
ORIGIN      
        1 tgctatcact tttagttttt aggataaaaa gtgacgtcac tttttgagtg ctggtacctc
       61 aaatcctgct ttttcttggg caatcaataa atctaacgca atacacataa tcctggattc
      121 acttttgccg tacttctgct ttagttgctg caattctttt tgtctctttt catccaacgt
      181 cacgggtaac cgtttatttc ctattgcaat tggcattagt actctctcct ttcatcgaaa
      241 taaccctatt aaaaatatgg ctgcaatcaa cttgaatatt agactcacaa gggttaacac
      301 aaagcgccaa aagatcaaac aaaataccag tattcctaag aatactacga agtttaaatg
      361 tagagctgca ccatgacgca taagtatctc gccaacaaac agtacgaaca atagaggaat
      421 gattttattc atatggatct ccttctttct cttaggttat cgggctttgc ccgcttattt
      481 cgcaccacct aagcggcggg ggcggttgga tcgattggtg cgaaataaga accattccct
      541 taatttcaag gttttttgtt cttatttcac acatcctcac ttttcggtat aaatggttat
      601 tgaccttttt tcctttcaaa tcgttgatat ggcattcgtt tacggtcaag ttcattttgg
      661 atttcttctg tatcgtcgta agtgtaggtg tctaaaaaat cagttaaagt cgtgtaccag
      721 tcgtcgcctg ggttatctac ttcttctaca tattccctca aaaatgcttc gggtacagtg
      781 agttcataat ccattcccat taacacgata gattccgtcc cacacgtcaa aaatctagta
      841 aatacaaggt aataatcgtc taccgtcgca tctttgctgt ttagctcttc catttcttct
      901 tccattaatt ccatgctctc aaaaattgaa taatattctg attctgagta atagtgtttc
      961 ctgcaaatcg tgtagctgac aaagttttcg accagctcac tcctttctag gtaaacattc
     1021 atgatatatt tttctgtttc tagtcgtaga agctccgctc ctttttttaa tgtgtcagga
     1081 cgacgtttat acacgtctaa acgattatgg ctgactgtaa ccttcgtttg actcgtttca
     1141 ctttccatat acaactgttc ttccactctt tgaataaagt acttcatttc ttccatacaa
     1201 ctaaagtctt tttgaatccg tctgattact tcattcaagt gttctttgtt tgttaaaaat
     1261 tgcgaaacat cttctgttgt tagctgataa accaccttat tcattatctt tctcctccga
     1321 ttcaatcgta taaaatgcac catttttttc gatattttca aaagtatcta gccagtcgct
     1381 agccattgct ttagctttct taatctgtgc gaattttgca taagtcagtt ctaaggcttg
     1441 atattcggaa ggtttgatag tgcttggttt gacacctact tccaagcata gtaaggcttg
     1501 gaaaaaccat tttccatttt gttttttcag ttcttccagt gttcgatagt acgtcgcaat
     1561 gagattcatt tcgacacctg attcaatcat ttcttccact gcgactgaca atttttcctt
     1621 gtaatgtttt ttgggtacac gtgtttcatg gtataggaac gtcatatcct ccattttttg
     1681 tgccatttta tccaaaggta atttgacaaa ttcgtcatag taattttgag ccatattttt
     1741 tctccttttt agttctctaa tttggttaac caatattcga tttcataagt ttccatgttt
     1801 tcttgataat gcagttcata tcctgcaatc ttcactaggt cttcaaatag catagtcggt
     1861 gcatcctcta ctaaaggggt ttctatcgtc cagtaccgcg gaataagtaa agcttctgct
     1921 actttttcag aaatgacttc tttgtccacc aaaatttgaa cgacttcttt tacagtcaat
     1981 aatttgtgtt gtgttttcat caccgtttct ttactttctc atcaatcaaa acataatcgc
     2041 cataagacaa ggtatatttt ttcccgcatg ttggacattg atacaaacga tagcttttga
     2101 tacatttcgc tctttctcca caatcgcatt cgacagtatg tggtgcatga tagtttggga
     2161 ataattcgct gaagggaaca ccccaataat caaatttctt tcgcatgatt gattctgcct
     2221 cctcttagga aaggttttat ttcctttcgt tagttctttc tttattgatt tgttggccag
     2281 aatgaatcaa cgaaaagcga caaggacaag ggcgcgcagc gtgccattta cccttgtcct
     2341 tgtcgctttt tggtgataaa aggcaacttg ttgccggtct ggcaaatcaa taaagagata
     2401 gcgaacagga acgaaccttt tggtgagagg aagttttttc tattttgtct tgctttgcaa
     2461 gacaccatat cttagtgaac gtgcaacgtt cacttccttt cttctcgaac ggcggtggcg
     2521 gttggagtga gagaaaggtt tttcaattga gaaggcactt gtgtcttcct aaaaaggtct
     2581 tttcctgttt ctatgtaaaa caaaaagagc tgcaatcctc atttaggact gcagctcttc
     2641 ttttatatgc ccatcgatcg cgtaaaatga tcggggtttc taatttgcat cgattcaatg
     2701 actaattgat tccgtttatt gaaatgacct gtgacggcaa tattgtattt tccatcatcc
     2761 ataattaaca atttatcgat aatttcaata tctgcaacta cacaattaat cgattcatga
     2821 attgtttgta aagtaaagcg aaccaataat ggttgtgttt ttattactcg gattttatca
     2881 agcaaaccaa catatttgtt catcacgatc acctcgctaa aattatagca aaaacagcta
     2941 aaattgagaa tttttattcg attttagata tgctataatg atcaagacag taaagtttgt
     3001 gacggtggct atccgattga aagtgaggtg gtgcgtatgt gcgtacttcc aaaatcttac
     3061 agaaaggaga agccttttgc ctgttgaaac ggcgctagga ttaatgatta gtttcggttc
     3121 tttgatcgca acaatcattt ttggaatctt agcagttgta aacaacaatc aaaaaaaata
     3181 accgtcagct ttagccggtc gacgattatt tttcaaaaat aatattaagc tggtcaccgt
     3241 cttaaacggt gctgttaggg gagttgttag cgcaactcct cttttcgtat ccattataac
     3301 aactttattg atcttttaca atcagaatgg taaatcatca tcactaatat cgattgattg
     3361 tccgccaaat ggattttgtg aagcgttcaa tccattgttg ttaggcgtag ctacatcccg
     3421 ttgttcaaag gtggtagggc ttggactgct ggacgattga tcagcgtgcg cacggctttc
     3481 tgtcgctgct ttactttcta atagttggaa attatcggcc acaacttctg tcacatacac
     3541 acgttgtcct tgttggttat cgtaggttcg ggtttgaatc cgtccaacaa cgccgatcaa
     3601 actgcccttt ttagctaatg tcgccatcgt ttctgctggt ttgcgccaaa tcacacagtt
     3661 aataaaatca gcttctcgat tgccgttttg attcgtgaaa tttcggttga cagctagggt
     3721 aaaagtcgct actgcagtac cactcgcggt ataacgtaaa tctggatctt tggttaagcg
     3781 tcctactaaa gttacattat tgatcatctt tatctctcca tttcttgatt catttgttgt
     3841 gatcttttaa actgccgttt tctttcttga ttttcaattt tgatcacact tttcattagg
     3901 tgatcaattt cgttgactgt atctgtaaat gaaatggaat tcgcaaacgt gttaactgag
     3961 cgatattttt cccactgctt ttccaaatag tcatttcctc tgatcttatc catttctctt
     4021 tcataataaa aggacaacgg ttttgtatcc ccacgttttg taaatgtatt tcgaacactt
     4081 ttgtataact ctttcgtatc gatattttct agtttcggaa tggtaaataa gtcgtagtaa
     4141 tcttttgctc tcgaattatc tgtgccaaag ctgaacgtag cacttagctt ttctgcaata
     4201 atttgttctg ttggataagc tggaatctcc acttttttat cttcaaacat taattggtga
     4261 tagtgtgtat ctacttcagg ataaatagaa tctcctgttg tcacgtccat tttaaacggt
     4321 acacgtactt tctctaaatt agccaccaat ttaatttgaa atccaggata aaaatcggct
     4381 tctcttgttt ccttcattcc ttgaatagag aaaacaatgc cttcttttgt gggttttcta
     4441 gttaaatcat taaaaatgct cttcaatttt tcttcagtta atttgctatt tctaaatgtc
     4501 gtatcgatat caattgttgt ccttctatcg attccatatt ttgatcctaa taaaaagccg
     4561 cctttcaaaa tgaagtcatt tttgtacttt gattcagaaa tttgttcaat tagacgatcc
     4621 atcataaaat gccgttgcac taactgtgca tttaatcctg tactctttgc taaatttctt
     4681 gtttttgctg caaattgtgt cggtgtcatc tttttcctcc atcaatataa gctttgcatg
     4741 tggtatttca tttctttttc tacatgtagt tttgtcatgt aatctcttaa tttacttgga
     4801 tctctcaatg ggtcttccat gtactctttt aatgcttcta atttcatttc atattcaaat
     4861 tcatatctcg gattccaaat atcacaaagt gtacgctctt tatcatacat agtaatagga
     4921 tttccttgaa aggaagttcc ctctactttt cctagttcat aaacttcttt cttcacgaaa
     4981 tgaaattgta actgctgctc ttttgtaatt gaacgatcaa cgttatagcc atggggaaaa
     5041 gttaagtgaa tgaatttcgg catattcgta gaaagctggt aaatatctaa agtcgtttca
     5101 tgagagaaga ttcccctgga aaatcgttgc tgaagccaaa ataaatcatc tattccatga
     5161 ccatcctcta cataataacc acgatcaact ttgatcaatt ttccttgatt caccaatctt
     5221 tgtacagtgg ttgctgcgat tccaaatttc ctggcttcct tcatagataa atttccatcg
     5281 cgaatagcca acaattcttt cagtttatct tgcatttact tcccctccct tcgttacaaa
     5341 tacactacat caaatatatc atagtagttt atttgtaaca agattaacgt ctatcgatct
     5401 aattcgattt ctttttgaat gcgctgctgg ttctttaatt gttgcagtct tcttttattt
     5461 gcacgtaatt tctcttgttc taaacgaatt tttgcttttt gattttcgat ttcttgttgt
     5521 ttgtgtgttg tatttcgcat aggataaccc cttcattatt ttgtagtgcg gcgtgttctt
     5581 ttcttatagt tcgttttttt cttttcagaa aattcaaagc ctgttgtaaa gtcttctttt
     5641 aaaacgatcg ttgcttcaaa cgaacctttc tgacctttta gtcctctgac tgattggctt
     5701 gttcggccgt tttgaatgat ctctttcagt tgtgtggctg tgagtttctt tttggcaatc
     5761 gttgtccata atttaaaatc acaggcttca gacgtacatg ccgccacatt ttgataaagc
     5821 aagacttctt gttcccgaca ttttggacaa acacctaaat gacgcgttgc ttcctgtttt
     5881 tcactggctg ctttcacctc ttgtaacgtt gatccaaaat ctgttgactg gatttgtccc
     5941 ggtacgactt ccaacaagtg agagacaaat ttttggatat tagctaagaa agtagcttgt
     6001 gttcctttct gctcaccaat ctttaataag taactttccc atttggcggt catttcggca
     6061 cttgttagca gcggctcttt ttccacggct tggcaaagta aaatcccttt atttgtcacc
     6121 acaagcttat ttttttctac cttgatatat tccttttgtt tcaaagtttc aataatattt
     6181 gctcgtgttg cttctgtacc gattccttct acttctttca atatggattg tgcttcttcg
     6241 ctatccacgg tttttccagc tgttttcatc gctgtgatca acgtaccttc ggtataaggt
     6301 tgtggtggct tcgtttcttt ttcaaggact tgtagatcta ccgtcaccgc ttctcctttt
     6361 gtcactttag gaagtagcgg ttcttcttcc ttttcttttc tgtcgctctc tttgccaaac
     6421 agctttttcc aacctagatc tactggtact tttccgactg tctttagctg aagctgctga
     6481 acttgcgtca agatcgttgt ttcttcatag gtatactttt ccgcaaacat ggccaccgtt
     6541 gtttttaaca cttgttcata aatagcctgc tgcagtggac ttaattgttc aaaagccgct
     6601 gccgtcatga ccgtctttgt aggaatgatc gcgtaatgtt cctggacttt cttattattc
     6661 acatatcgac tattcggtgt atgaatagga gtaggaatgg cttcggcctt taaaaagtgt
     6721 ttgtattcgt ctaagtgatc caacagatac gcatactccc cttctgtaat ataaggggta
     6781 tctgttcgtg ggtagcttag atatttccct tcatacaacc cttgcatggc ttccaaagta
     6841 tctttggcac tagctttcat gagctgattc attttagatt gaaggctgga tagagaaaaa
     6901 agacgtggac ttgccgcact ttttttctcg gtttcaactt ggtggatcgt acctggctgt
     6961 tttcccaatt gaaccccctt ttcttttaaa taatcttcaa atgcttcttg cgtcgcttgg
     7021 gtttcattcg gatcaagctt cgcatcaaat gcgccatttt gagcgattac ttgggctttt
     7081 ccttcaaaat atggttcttt ttggaagttt ctaatctttt cctgtaattg ataaatcatg
     7141 taaagcgttg gggtttggac tctgcctaag gaaaaacttc ctggtatgcc tttttgctgc
     7201 aagagtaaac tatacagcgg gctaccgttc atgccgatca accaatcggc gatctggcgt
     7261 gcttgtgctt cttcaaattt tgggtaggtc tcttctgcat ctcgtaactg ctggaatccc
     7321 tgataaatgg cttctttttc taaggagttg atccaaagtc ttttaaatat ctttccatga
     7381 tcggcgcctg cttgttgaat gatcgtccaa gctatcagtt ccccttcacg atcgctatct
     7441 gtcgcaataa taatggtgtc ggccgattga agttgctgtt tcaccacctt gaattgcttt
     7501 ttcttatctt taggaacatg atagtgataa tgtgtaggga aaataggcaa atgctctaat
     7561 gaccattgtt tccaattttc atcgtacata tcgggcgaat ctaactcgac taaatgacca
     7621 aaaccatacg tgatcacagt ttctcctgaa aacaatcgat cttggatctc aaaataccca
     7681 tcttttcggg tagctttaga aaagctatcg gcataagcct tcgcttgtga cggtttttct
     7741 gctaaaataa cggttttcat catgacgatt tcactccctt tcttgctgtc taccttgttg
     7801 aaacaacatg gcacgttttt gctttctgat ctcttgttgg tagatttcat gcttcaattg
     7861 atcattccaa tcctttttta cttcaggtac aaattcatgt tcatgatttt tatagccttt
     7921 gtagtattgt tctgctttat aagagaaaag aatctgagca ttaatatccc ctttttcttg
     7981 ataaaaacga tcaaaatcgt acgtaccatc cacgcgttca caagctttta attcttttgc
     8041 gacttctcta caaatatctt tcaaattaat agcttctatt gaatcgttaa cagaaagctg
     8101 ctgaccaaaa gacgcattta ctttgtgagc ggaaaccaat tggttcgtta attgcagatt
     8161 gttttctgtc ttgtgtatgg ccataatagc ttctggtgtg atcttttctt cttttgcgac
     8221 ttcttcataa atgactttgt attcttttgg cacttgccaa tcattagcga ttcctcgttc
     8281 acaacgtact ttctgattcg taaatggatc aactgcgccc attaattgtt ctttttcgta
     8341 gaaaatatgt cctgcacggt cattatccac acaaatctcg atggattgag gaaaggcttg
     8401 tttctttctc aattcagata ctgcctcacc aaaataatga ctgatcacat ggtgttttaa
     8461 cccttccatc gaaactaacc acgtatcgtt taactgatca cgatttagtg cagcatagct
     8521 aagtaaatca atactactct caaaaaattt taactgctta gggtcaccga tctttaggtt
     8581 aaagccatga ttcgctgtcg aatttttatc tatatgctta tatgtgcctc tctcaccaaa
     8641 acgttcataa tcaatgaacg ttccttgata actcgcccct actgtctcat ttgtctgtgg
     8701 atctttccat aaaaaggcga ccgcatgatc taaacgatcg tctcgaacta agaaattttg
     8761 ccgttcatgt tttggcttta gttcggcaat caacccttct ttttctgcat aatcaatcag
     8821 ccactctggt agttttcgtt cttccttcag ataattcctt cctttttctg tgagtggttg
     8881 ataatcaacg gggtgatctt tgtacttttc tgtctcaaaa tgaagatttt ccactcgttc
     8941 tgttttgtct tcattcttta gaataaaatc agtggctgct ttaaattgtt gcttagggtc
     9001 ttggatctct ttgtctatga aaaaaagttt agctaattca ataatgtcgc cactaatatt
     9061 ttgactgttc caataaaaac gttgttttct tcgatcaaaa acaaatgaat catggtcttc
     9121 taagcgatag tctcgtcctt tattgactac agccattcca ttgtttcgag caaaatccac
     9181 tatatcgatc tgtttacatc gatcaataag actcattttt ttctcggtca ttttaaaacc
     9241 tcccaaaaga aaaaagacta tccgtttttc agatagtctt ttttacttgt tttatacacg
     9301 ttctttttgt gacaattgtt gttcataata gatttcattt tttcgttttg ccacttctga
     9361 cactgtctca agtgaaaatc ggaatgaatt tcctagcgca aattcttctg aaggtaacgc
     9421 cgtttggatc tcttttccat aactttttcc ttttaaaatt acttgattat gctgtggatc
     9481 ttcaataaac gccgcatcct tcaaatgtaa cgttcgaccc gtatcttctt ccattacttt
     9541 cccgtctttc ttgtcgatcc aaagtgaacc aacaggaaca atattctttt gttctgccgt
     9601 ttctacagta tttttatgct cctgcttcac ttctgctggt tggatctctg gttctctttc
     9661 tctgtattga ttgatttcat tagaaatggt ttgaatgaat tcatttgcga cacggctgac
     9721 atcattcatg attttcccac gttccgcagc ttccttatca tccaacgtct gaccattctt
     9781 cgtccatgct gcaatataag gaatagcttt ctccgaagtg tccattccaa aatgtttgca
     9841 cacaatataa gaagtcattt ctgcctgcaa ttctttagtt gcggtatcta attctgaaaa
     9901 tttgtgcatt cttgcatggg ctaattcatg aatacttgta gctaaatgct gcgtaggagt
     9961 atttcgagta ttcatgacga tttcttcttc gccagtaaat tcgttacgaa catatgcccc
    10021 ccgtgcttgt cctaattctc tttgatagac ttcactttct gtcatatctc gaatttgtat
    10081 tccaatattg ttggctaccg cttgaatccc agcttgaagt tctgactgtc cttttttatc
    10141 caattgaaaa tcaaagacac ggttcggaaa gattttcgga tagtcttcag gttttaactg
    10201 tgtttgtgtg atatcaaaca ctttttcaat cgtgtagtaa ggttttttga tcacttcgat
    10261 ttgaccactt tcgattcgtt ttttatcttc tggtgtagct tctgtttctc gaacaaagcc
    10321 acgtcctttc tcataaaatc ctttgaccgt ttttctgaca aagatcttgt tcccttgttc
    10381 cccttttttg atatagattc cttctttttt caacgcagca cgacctaaac aagccgttgc
    10441 acctggaaat tgttcctcaa tcaataattg atttctaaag gatcgttctg gaaaacgaga
    10501 cataaaattc aaaagttctt tcacgtcttc aggagaatgg gtatgctttt tcaccgcatc
    10561 cagtgcctgt tgcgaaaggg atttgacttc ttgcttaatc tcttctgccg ttcgtttttg
    10621 atagcccttt tgttttgttt ttgtttcttt cgactgcatt tgttctttcg aaacaggttc
    10681 atttttgacc gtttgttttt gttccttttc aggtacttgt tcgcacaatt ttgctaactc
    10741 taaatagatg ggttgattac tgttgacgcc atcccctaga tccattcgga tctgatcgac
    10801 tacttctccg ttcttcactt cttcaaaata gaatttataa taacctgctt ctgcggcgta
    10861 ttggttatcc aacgttttga gttgatcgat caattctaat gttacgatct ccccaccata
    10921 acttttcaat cctttttctg gatcattttc gttgaattct actcgataga aatgatcgtt
    10981 ttctttctcc ttcaaagaat cagttggcgc agcctcttca agtaattcac gaacagctgc
    11041 tttttcctct tccgacgtgg cttggtattt ttttgattcc tcaaatcccc cgttttcatt
    11101 cagttctaaa gcaacttttc gatagtcttt caaaaaataa atggttggat tttttttcca
    11161 ttgcgtcact cgtccgctgt caataattgc ttgtacttca ggaagaagat tttcttttcc
    11221 tttttctgat ctttctttca gctgttctaa tgtttccaat ttccgttggt attttcggat
    11281 cgtagcggct gaaaaagtcg attcaccttt ttcaaagcgt tcgatttctt ctttgattct
    11341 tggaatattt tcgattgtca tatccaggcc gccgtacttg ttatagccca tttcctttag
    11401 ctcttcttgt ctttcaagtt tttctacctg tttttcttgt tgcttgatct cttctctcag
    11461 actatgcact ttgttttcta aacgttcttg ttgcctaaac caagaatgcc catttctctt
    11521 atcgttcatt ggctgaccgt tggtctttct ctggtgttca aacatcttct caatttgttc
    11581 gtttaattca ttttgcaatc gtactaattt tcttttggat tttcccaagc gactattgtc
    11641 tagtggttct ttttcttctg ccatgtcc
//
LOCUS       DACDXO010000076        10921 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E23605_2017
            SAMN12639989-rid9881463.denovo.077, whole genome shotgun sequence.
ACCESSION   DACDXO010000076 DACDXO010000000
VERSION     DACDXO010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639989
            Sequence Read Archive: SRR10023389
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741914.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:42:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,972
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 33 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 17 of 73
            Pseudo Genes (multiple problems)  :: 10 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10921
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639989-rid9881463.denovo.077"
                     /isolate="E23605_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..287)
                     /locus_tag="IWM35_002366"
     CDS             complement(<1..287)
                     /locus_tag="IWM35_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9920194.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEAE"
     gene            429..1547
                     /locus_tag="IWM35_002367"
     CDS             429..1547
                     /locus_tag="IWM35_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP9920195.1"
                     /translation="MFEKLRQSKLMFWSLELLIIAALIMISSEINFIFEPIGTFFSTL
                     FAPVLIAGFLYYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQI
                     SSLASNMPDFIKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSL
                     GSIVGTIASATVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTL
                     ANYISGQAIECLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLV
                     TVFNDPVKALLCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLG
                     IFLGVPFYAICKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            1675..3051
                     /locus_tag="IWM35_002368"
     CDS             1675..3051
                     /locus_tag="IWM35_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HAP9920196.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            3452..5032
                     /locus_tag="IWM35_002369"
     CDS             3452..5032
                     /locus_tag="IWM35_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HAP9920197.1"
                     /translation="MNNPKLKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            5230..5453
                     /locus_tag="IWM35_002370"
                     /pseudo
     CDS             5230..5453
                     /locus_tag="IWM35_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
     gene            complement(5658..5978)
                     /locus_tag="IWM35_002371"
     CDS             complement(5658..5978)
                     /locus_tag="IWM35_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HAP9920198.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPEH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
     gene            complement(6126..8360)
                     /locus_tag="IWM35_002372"
     CDS             complement(6126..8360)
                     /locus_tag="IWM35_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HAP9920199.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            8839..9105
                     /locus_tag="IWM35_002373"
     CDS             8839..9105
                     /locus_tag="IWM35_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HAP9920200.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            9105..10832
                     /gene="ptsP"
                     /locus_tag="IWM35_002374"
     CDS             9105..10832
                     /gene="ptsP"
                     /locus_tag="IWM35_002374"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HAP9920201.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDMMEEV
                     VELVHEYVK"
BASE COUNT         3474 a         1911 c         2214 g         3322 t
ORIGIN      
        1 ttttctgctt cttcaatcaa tatttttcct aacccttggt gtctgtattc agaaagaatc
       61 gccatccgct gtaatttcat ttttccgttt tctaaaggca aaagacgtac agttccttgc
      121 ggttcttttt tatctgagta caggacgaag tggatacaat gggcttcatc tttgtctatt
      181 tctcgagaca aaggcacacc ctgttcgact acaaaaactt gatgcctgat ccgtacagca
      241 tctaagtaaa tattgctcat tgtatctttt gtttgtacaa ctttcatggt ctagccacct
      301 tcctttcgta tagtatactg gaaaataaaa caaataaaaa gtatggcaag ttttcaaaaa
      361 taaaaaaaga ataaaagaat ttttaacgaa tataatatat actagtaaga aagaaaggag
      421 gaaatctatt gtttgaaaaa cttagacagt ctaaactaat gttttggtcg cttgaattat
      481 tgatcatagc tgcattgatc atgatttcat cagaaataaa ctttattttc gaaccgatcg
      541 ggacattttt ttccacgtta tttgcacctg tactgattgc aggctttctt tactatttat
      601 tgaatcctat tgtcaagctg ttgatgaaag caaaaatcaa aagaatatgg gctgtcgcaa
      661 ttgtgctgct ccttttagtt gcagcaatta tttggatttt acttagtgtc atcccaagtc
      721 tagtacagca aatttcctca ttggcatcta atatgcctga tttcatcaaa caggtagaaa
      781 gctggttaaa agaggtagcc gaattgcctt tgttcaagga agtagatatc aataaatatt
      841 ttgagcaatt agacatttct tacgggacaa tcattcagca attccttagt ggagtatcca
      901 atagtttagg ttcgatcgtt ggaacgatcg cttcggcaac cgtagtaatc attacagcac
      961 cattcatctt gttctatatg ttgaaagacg gagaaaaact agtgccaaac atccaacagt
     1021 ttttcccaga aaaaagaaga aaccaaattg tggagttatt ggggcaactg aataaaacat
     1081 tggctaatta cattagcgga caagcaatcg aatgtttatt cgttggtacc ttcacatttt
     1141 taggatatat ggtaattggt gtggattatg ctttcttatt tggggtcatt gcgggattga
     1201 caaacctaat tccatattta ggaccttatc taggactagc acctgcattt ctagtaacag
     1261 tcttcaatga tccggtaaaa gcattacttt gttgtgtggt tgtgctaatc gtccaacagt
     1321 tggatgggaa tatcatttat cctaatgtaa ttgggaaatc actgaaaatc catccattaa
     1381 cgatcattat cgttcttcta gtagctggaa atattgccgg attgcttggt atcttcttag
     1441 gtgttccttt ttacgcaatc tgcaagacga tcatttccta tataatcaaa atcgtcaaag
     1501 aagacaagca aaatgaaaac aagaagaaaa ttgctacgac ctcgtaggta gttttagtac
     1561 acttgttttt attgcaaaga aaattgcttt gtgataccat tttaacgtgg ctatagtgcc
     1621 acgttttttt ctatcgtaaa ctaaaaatta attttataag gagagaatgc gattatgaat
     1681 gctgaccctg agagtcagtc gctgttagcg cagattttat tattgatcgt tttgacattg
     1741 atcaatgctt ttttagcagc ttccgaaatt gctgttgttt cgatcaataa gaatcgaatc
     1801 gagcaaaaag ctgatgaagg agatgtgaaa tctcaaaagc ttttaaaaat attgcagaat
     1861 ccaaataatt tcctatcgac aatccaagta ggaatcactt tggtcaacat tttgtctggt
     1921 gcttcactag ctaatacttt atcggaacgg ttagctccgg tattaggtgg cggcacagcg
     1981 gcaaaaaata ttgcaaatat cattgtgctg gctattttga cctatgtatc gattgttttt
     2041 ggagaattgt accctaaacg tattgcttta aataaatcag aagaagtcgc aaagttcact
     2101 tcagggctga tacgcttgat tggtgtagta gctaagccct tcgtctggct gctttctgct
     2161 tccacaagtt tattggcgcg tatcacgccg atgacttttg atgatgaaga ttcaaaaatg
     2221 accagagatg aaatgcgtta tatgcttgaa aacgaaggcg tattgaataa tgaagaatta
     2281 gaaatgctcc aaggggtttt ttctcttgat acgaaagttg cccgagaagt catggtccca
     2341 cgaacggatg catttatgat tgatatcaac gacactgtgg aagaaaatgt gaatgaagtt
     2401 ttatccgaaa actattcaag aatccctgtt tataacgaag acaaagataa agtagtcgga
     2461 atccttcata caaagaattt actaaaagct gcccataaat tcggctttga taatttagat
     2521 atcaagaaaa tcatgcagga gcctttattc gttcctgaaa cggtctttat tgatgatctg
     2581 ttatatgaaa tgaaaaagac gcagaatcag atggctattt tattagatga atatggcggc
     2641 gtcgttgggt tagcaacact agaagacctt ttagaagaaa tcgtcggaga aatcgatgat
     2701 gaatcagatg aagttgagaa tctttatgaa aaaatcgatg aacacgaata tatcatccaa
     2761 ggacgtatgc tgattgatga gttcaatgaa gcttttgaca gtgatctgca tatgagtgat
     2821 gtagatacga tggctggtta tttgattaca gcattaggca tgattccgga tgaaggcgaa
     2881 aagttatcgt ttgatgtcga taatattacg ctggtttctg aagaaatgga aggttctcga
     2941 atccttaaaa tccgtgtcat ttttcatgat ccggaagaaa cagaagcgga acctgaagaa
     3001 gaacgaagat acttcagaaa agaattcgaa gatgacgagc caagaagata attggaaact
     3061 tggttatgaa agacgtgttg cttgaataaa caagcaacac gtcttttctt ttagtcagct
     3121 gacaaaaaaa tatttgactc ttatggtttg gtggcttttt cattcgctga atccatgcta
     3181 tactgattca gttgtagtag ttgacaaagg ttgtgaaaaa gctatcctgc attaagtaat
     3241 aagagcagcc aagcaaaaac agcttttcgt atttttggct tgggtgggct tattgctgat
     3301 aggctgtctc tagaacgccg tttactcgga aaaagagact gctacacggc ttttgtcaca
     3361 gccctatatg aaattgtctt tctttaagag atcggacaat ctgtgcctag agaaaatata
     3421 aataattgaa tggaagaaat ggagttaggt tatgaataat ccaaaattaa agcaacaagt
     3481 ggacaatcgc agaacttttg cgatcatttc ccatccggat gctgggaaaa caacgattac
     3541 agaacaatta ctattgtttg gtggggctat ccgtcaagcc gggaccgtaa aaggcaaaaa
     3601 aacaggtaat tttgccaaat cagactggat ggaaatcgag aaacaacgtg ggatttctgt
     3661 tactagttct gttatgcagt ttgattatca agggaaacga gtaaatatct tggatacacc
     3721 aggacatgaa gatttttctg aagatactta tcgtactttg atggccgtgg atagtgcggt
     3781 catggtaatc gatagtgcaa aagggatcga agcacagaca aagaaattat tccaagttgt
     3841 aaaaaaacgt ggcatcccga tatttacttt tatcaacaaa ctagatcgtg atggtcgtga
     3901 accattggaa ttgttagaag aattagaaga gctattagat atcgaatctt atccgatgaa
     3961 ttggccaatc ggtatgggta aagggctaga aggtctttat gatatctacc ataatcgtgt
     4021 agagttttat cgtccggaaa attatcagga tgaacgatta gtagaattgg atgaagatgg
     4081 actgcttccc gaaaatcatc ctctaactga aaattcctta tatgaacaag tcttagaaga
     4141 agtagaacta atcaaagagg ctggagatgc atttaatcaa gaaaaaattg ctcgtggcga
     4201 tcagacacca gtattttttg gttctgcatt gactaacttc ggtgtacaga cttttttaga
     4261 gactttcgtt gaattggcac cagctcctta tggacacaaa acacaagatg atcagatggt
     4321 aagtccttat gaggaagaat tttcaggttt cgtattcaag atccaagcaa atatgaatcc
     4381 agcccatcgt gaccgaatcg cttttgtccg aatctgttct ggaacattcg aacgaggaat
     4441 ggatgtctgg ttggaacgta caaataaaaa actgaaatta agcaatgtca cacaatttat
     4501 ggcagattcg agagaaaacg tcgaaaaagc agtagcaggc gatatcatcg gtgtatatga
     4561 tacaggcaat tatcagattg gggatacatt gttcgaagga aaactaaaag ttgcttatga
     4621 agaactccct tcatttactc cagagctgtt tatgaaagtg actgcaaaaa atgtcatgaa
     4681 acaaaaatcc ttccacaaag gaatctatca attagttcaa gaaggagcga ttcagcttta
     4741 taaaacatat ctaacggaag aatatatcat tggagctgtt ggtcagctac aattcgaagt
     4801 attccaatat cgtatgttaa atgagtataa cgccgaagta atcatgtctc caatgggaca
     4861 caaaatcgct cgctggatcg atccagaaga tttggatgag aagatgagtt caagtcgcaa
     4921 tatcttggct cgtgaccgtt tcgaccaacc gttgttctta ttcgagaacc aatttgcaga
     4981 acgctggttt gcggataaat atcccaatgt aaaattaaaa agtttgatgt aaagaagtct
     5041 tgacacaagc tgccctgcat caagtaataa gaatagccaa acaaaaacag cctctcatat
     5101 tttgtttaga gccgtgacgg attttccgtg acggctcttt ttactatatt aaaatcatag
     5161 aaatgtctaa tcaaaaaaat ataaaaatta ttttgcgtac atatgattaa agaaaggaga
     5221 gtgtgacaca tggaaacaga atggcaaaga cgtgaaagaa aaaaacagaa aaagaagaga
     5281 tgttacgatt atgtcgatta ttagatgtaa aagactggga caaaaacgag caaattgcac
     5341 aaaaagtacg atcaatcatc cataaagagg aaaaagttaa aaataaagac acgcgtagaa
     5401 ctgaaaatga aaagagaaaa gtacaaaaga aaagagaaaa atcaaaaagc tagaaagaaa
     5461 aagaggctga gacagaagcg tttaactcca agaaataagc tggaattcac aaaaatttga
     5521 aaagcaattt tcgtgaattt cagcttattt ccgaaggagt tgcttctgct ttcgccgttt
     5581 atacgtgttt agagcgtgag acaaaagtgt tctttacttt tgtcccacgc tctttaatta
     5641 ttatgttcaa aatttgttta cacggctaat gtagcgccta attcatgttt tggaatcgag
     5701 atttcaacaa ggcctgccaa ttcgattact ttgatttcat cctcgtggta aacggcgtct
     5761 ttgattttat ctaagaaata atttttagca tcttcgattt ctttcggctg gattttacgt
     5821 ttgtcattga tcagcagaat ttcattatgt tcaggggctt cagtgtagac aatagtggta
     5881 tttccggctg tgtagacctt gactagatgg gcatcagtgt tttcaagctg gtttaataca
     5941 agacgggaat ggctgtttgt tacatttacc aatttcatgg cgctcacctc ttttatgtat
     6001 ttttaattgc ttgtttctct tattataatt tcttttttat aaaatgaaca gtattttgac
     6061 ttttctgaaa aaagaatgag acggcatttc tcaaagaaat accgtctcac gctataaaac
     6121 tggatttatt cagccgtttc ttcggctgat tctttagcca aacgttctgg tttagaagta
     6181 acgatgattt ttccatcttt atccagtttt gctttcaact catggataga agggtgatca
     6241 agataaaatt cagcgatacc atcttcgatt tgttcttgga tcgtgcgtcg aagaggacga
     6301 gcacccatag atggatcata gcctaagtca actaattttt ctttgacatt cgtaggaact
     6361 tcgatgtgaa gttgctgtgc tgccagcatt tggttgacat cgtctagcat tagtgtgacg
     6421 attgtcatca agttttcttt tgataatgca ctgaattcga tgatcccgtc aaaacggttc
     6481 agaaattctg gtgtaaagta gttgtttaat tggttcaata cagaacgagt aacaccttcg
     6541 cgtgccgcac caaaaccaac gtttgcttct actttaccag ttccggcatt acttgtcata
     6601 atgatgatcg tatctttaaa gctgaccgtt cttccttgtg catctgtcaa tcgaccatca
     6661 tctaagattt gcaagaacat atgcaaaaca tctggatgag ctttttctac ttcatctaac
     6721 aagacaagac tgtaaggatt tctccgtact ttttctgtta attgaccagc ttcttcgtat
     6781 ccgacataac ctggaggaga accaatcaat ttagagacac tatgtttttc catatattca
     6841 gacatatcga aacgaatcat tgaatcttgt gaaccgaata gttcgtaggc aagttgtttc
     6901 gcaagctctg ttttacctac accagttggt ccaacaaata agaaggagcc gattggacga
     6961 ttctgtttgt ttaatccaac acggtttcgg cgaatcgctt tcgctactcg atctacagcg
     7021 ttatcttgtc cgatgacatg agctttcaag tcatctgcta agtttttcag ttgtgtctgt
     7081 tctttttctt ttaattcacc cactggaata cctgtccgtt gttcaacgat tttttccata
     7141 tctttttcgg taatcacagg tgtttcctct tcagtgagct gacgttcttt cattttttga
     7201 agtttattga tctgatcgcg ataataagct gctttttcga aatcttcttc tcgagaagct
     7261 aaaacttttt gttcttctgc ttcttgcagt tttttctcga ttgttttagg atcaacgatt
     7321 tggatcgtca agtttttctt agaacctgtt tcatctagca aatcgatggc tttatccggc
     7381 aagaagcgat cttggacgta acgattggat aaagttgcgg ctgcttcgat ggcctcatca
     7441 gtatatttta catgatgata gtcttcataa cgtttttgca aacctttcaa aatactgatc
     7501 gtttcttcga cagttggttc atctacccgt acaggttgca tacgacgttc taaagcagcg
     7561 tctttttcga tgatgcggta ctcatttaag gtagtagcac caaccatttg caattctcct
     7621 cgagctaaag ctggttttaa aatattacca gcatccatat ttccatcacc agcagaacca
     7681 gcaccgacga tttcgtgaac ttcatctatg aataggatca cattttctgc ttgacggatc
     7741 tcttcaatca atttttgcat acgttcttca aattgtcctc gtataccagt accttggact
     7801 aaagatacga catctaaacg aatgacttct ttgtccatta atttttgcgg tacatcacca
     7861 tctacgattt tttgtgctaa accttcaaca actgctgttt taccaacacc tggttctcca
     7921 atcaagacag gattgttctt ggttcggcga tttaaaattt caatgactcg tttgatttcg
     7981 tcatcgcgtc cgatcacagg atcgatatca ccttgtcgtg cttgttctgt gatattgatt
     8041 ccgtattctc ccaataaacc ttctgtattg ccttgaggtg gctgaccacc attgaagttg
     8101 ttgtttccgc caaattgtgt tggtggtgtt tgctcataat ttccttgctg ttgcatttgg
     8161 cgagacatgg aacggaaaag atcgtccaaa ctgccaaatc caaatggatc attttgggcc
     8221 atattagtca gacctccatt ttgttgattt ttgatttttt gataacagct ctgacagtaa
     8281 tcgagttgtg tccgctgacc attgacagtg gcatacaagt gaatcgttgc ctcattcttg
     8341 ccgcagtttt gacaaagcat gaatcattca cgtcctttca caaaaatggg tacatccata
     8401 ttttacatct cgtttttcat gaggtaaaag aataacccct agttttttaa gagatttatg
     8461 aggtagaaaa ggtgaaatct ctattgacca ttcctgacct ttgactctta ttatactaaa
     8521 aataaaaagg gaagcaagta attcgtttca tatgaaaaca aagagggtca aaggaaagaa
     8581 tgataaacgt caggagaaga gaaagcgaca agaaaaagga aataaacacg tttgttataa
     8641 aatgtgaaga aaaggatgaa aaagaatccg gaataatttg aatactttta tctgaaattg
     8701 aatgaaaact gttgtatcta acgaaaaaaa atgttaatct ttacagatga aagggtggca
     8761 tttgttagga ttctctgaat tactaaaaaa gaaaaccctc acataaaaac aacactcact
     8821 taaaggagac cgattaatat ggaaaagaaa gaatttcacg tagtagcaga aactgggatc
     8881 cacgctcgtc cagctacact attagtacaa acagcaagca aatttaactc tgatgtaaac
     8941 ttagagtata aaggtaaatc agtaaacttg aaatcaatca tgggcgttat gtctttaggt
     9001 gtaggtcaag gttctgatgt tactattact gcagaaggtg ctgacgaagc tgatgcaatg
     9061 gcagcaatcg ttgaaacaat gaagaaagaa ggcttatctg aataatggtt gaaatgctaa
     9121 aagggattgc cgctagtgac ggtgtagccg ttgcaaaagc ttacctgcta gttcaaccgg
     9181 atttaacatt cagtaaggca acagtagaag atactgcggc tgaagaagct cgtttggatg
     9241 ctgcattagc taaatctact gaagaattgc agcaaattcg tgaaaaagca gcgcaaagct
     9301 taggtgaagc agaagcgcaa gtatttgatg cccatttgat ggttctttca gatccagaaa
     9361 tggtaggtca aatcaagcaa aatattaaag acaacagcgt aaatgctgaa tcagcgctaa
     9421 aagaagtaac ggatatgtat atcggtatgt tcgaagcaat ggaagataat gcttatatgc
     9481 aagagcgtgc tgcagatatt cgtgacgttg ctaaacgtat tttagcgcac ttattaggcg
     9541 ttactttacc aaatccatca atgatcaacg aagaagtggt cgttgttgca catgacttga
     9601 ctccaagtga tacagcacaa ttagatcgca attttgtaaa agcgtttgta acagacattg
     9661 gtggacgtac ttcacattct gctatcatgg cacgttcttt agaaattcca gcaatcgttg
     9721 ggacaaaaga aattactgct aaagtcaaag aaggcgatat tttggctgtc aacgggatcg
     9781 aaggcgatgt aatcatcgat cctacagatg aacaaaaagc tgaatttgaa aaagcaggtg
     9841 cagattacgc agcacaaaaa gcagaatggg aaaaattaaa aaatgcagaa actgtcacag
     9901 ctgacggcaa gcattttgaa ttagctgcaa acatcggaac accaaaagat ctagttggtg
     9961 tccataataa tggtggagaa gcagttggtc tgtaccgtac tgaattcctg tatatggatt
    10021 ctccagactt cccaacagaa gatgaccaat atgaagctta caaagctgta ttagaaggta
    10081 tggaaggaaa acctgtagtt gttcgtacaa tggacatcgg tggagataaa gaactaccat
    10141 atctgcaatt acctcatgaa atgaatccat tcttaggata tcgtgcattg cgtatcagtt
    10201 tatctgaaca aggcgacgaa atgttccgta cacaaatgcg tgcattgctt cgtgcatctg
    10261 ttcatggtaa tttacgtatc atgttcccta tggtagctac actcaaagaa ttccgcgcag
    10321 caaaagcgat ctttgaagaa gaaaaacaaa aattgataag tgaaggcaag gaagtatctg
    10381 atacgatcca agtcggcatc atgatcgaga ttccagcagc agcagtctta gcagacaagt
    10441 ttgcaaaaga agtagatttc ttctctgtag gtacgaacga tttgattcaa tacacaatgg
    10501 cagcagaccg tatgaacgaa cgcgtttctt acttgtacca accatataac ccatcaattt
    10561 tacgtttgat caaaaatgta attgatgcag cacacgctga aggaaaatgg gcaggtatgt
    10621 gtggagaaat ggctggagat caaacagctg ttccattgct agtaggtatg ggtctagatg
    10681 agttctcaat gagtgcgaca tctattctta aaacgcgtag tttaatgaaa cgcttggaca
    10741 cagcgaaaat ggctgaactt gctgatcgtg cattgaaaga atgcgatatg atggaagaag
    10801 tcgttgaact tgttcatgaa tatgtaaaat aaacaacgta tcaaaagctg gtgtggcttg
    10861 taaaagagct acgccagttt ttttgttctt ttttaagaaa acgtttttgt cagtaagaat
    10921 a
//
LOCUS       DACDXO010000077        10191 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E23605_2017
            SAMN12639989-rid9881463.denovo.078, whole genome shotgun sequence.
ACCESSION   DACDXO010000077 DACDXO010000000
VERSION     DACDXO010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639989
            Sequence Read Archive: SRR10023389
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10191)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10191)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741914.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:42:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,972
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 33 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 17 of 73
            Pseudo Genes (multiple problems)  :: 10 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10191
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639989-rid9881463.denovo.078"
                     /isolate="E23605_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            119..625
                     /locus_tag="IWM35_002375"
     CDS             119..625
                     /locus_tag="IWM35_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HAP9920202.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            642..1541
                     /locus_tag="IWM35_002376"
     CDS             642..1541
                     /locus_tag="IWM35_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 and DUF4163 domain-containing protein"
                     /protein_id="HAP9920203.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            1628..2512
                     /locus_tag="IWM35_002377"
     CDS             1628..2512
                     /locus_tag="IWM35_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HAP9920204.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNHTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
     gene            complement(2704..2775)
                     /locus_tag="IWM35_002378"
     tRNA            complement(2704..2775)
                     /locus_tag="IWM35_002378"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2740..2742),aa:Arg,seq:ccg)
     gene            2835..4169
                     /gene="rpoN"
                     /locus_tag="IWM35_002379"
     CDS             2835..4169
                     /gene="rpoN"
                     /locus_tag="IWM35_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HAP9920205.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            4372..5409
                     /locus_tag="IWM35_002380"
     CDS             4372..5409
                     /locus_tag="IWM35_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SorC family transcriptional regulator"
                     /protein_id="HAP9920206.1"
                     /translation="MLDEIKMIESVAPDIIEVLQERYKILRNIYWMQPIGRRSLSESM
                     GITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSLENFMDQLLGTHQTEQQLA
                     EYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEGENIIAVMGGTTMAVVAEQ
                     LSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKANGNHRALYVPEQLSLATYN
                     SLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEKELVMLKQANAVAESFGYF
                     FNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAIRAYMKNAPKQTWLITDEA
                     AANEILKGVTL"
     gene            5450..6451
                     /gene="gap"
                     /locus_tag="IWM35_002381"
     CDS             5450..6451
                     /gene="gap"
                     /locus_tag="IWM35_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HAP9920207.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            6561..7751
                     /locus_tag="IWM35_002382"
     CDS             6561..7751
                     /locus_tag="IWM35_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HAP9920208.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            7855..8610
                     /locus_tag="IWM35_002383"
     CDS             7855..8610
                     /locus_tag="IWM35_002383"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HAP9920209.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            8726..10024
                     /gene="eno"
                     /locus_tag="IWM35_002384"
     CDS             8726..10024
                     /gene="eno"
                     /locus_tag="IWM35_002384"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HAP9920210.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
BASE COUNT         3565 a         1886 c         2111 g         2629 t
ORIGIN      
        1 tttgtcacac cttctttttt gtatataaaa atcccaagta aatacataaa aaataaaaaa
       61 attgaaactt ttagcgcacc ttgtacgtca tattaagtga ggcagaaagg agtggagaat
      121 gatttttact ggatataaga aaaaacggca gatcgaaaaa taccgtcacc tgctgaatgg
      181 actcatcgaa actgattatc aaaaaatgtt ccgtatcacg atgaattacg tacatgataa
      241 agaagaggcc ttagatgtga tgcaagatag ttttcataaa gccctgaact attttgaaaa
      301 agaaaaagag atcgagcatt ttagtgcatg gttttatcgg atattgatcc gcacggcatt
      361 agatagttgg cgcagacaaa aaaggacgcc tgtggactta tttgaaattc aagatatccc
      421 ccaacagtta cctttagaaa cttccgttgc agagctccat atgattttag ataaaataga
      481 cagtccggaa aaagagattc tgatcctcta cttttttgaa ggatttcgtt tgaaagaaat
      541 tgcgactatc ttggatatga atgaaaacac agttaaaacg aaaatgtacc gctcattgaa
      601 tgctttacgt caaatcctag aatgaaggag agaagagtgc catgtattcc caaaaagaaa
      661 atttaaaaaa actaaagaaa acttataaag aacaagcaat tcccaaaggg cttaaagagg
      721 agatccgcca gcgttttatt atcgaagagc aatcatttat aaaagcaaag cggagaaaga
      781 gacgtctcca agggttcgga gtatctttgg ctatcgtaat gattacaaca agtagccttc
      841 tttttaatag tcaagttcga agctttgcag aagatttacc catcttagga tcagtcattg
      901 aattgatctt aggcgaacgc tttactgatc gatctgaaaa aatcgatatt caggtaccga
      961 agatcaatac acaaaatgag aaagaaaata aaacaattca tggattaaat caaaaatact
     1021 ttcgcgaagg acaagcagat tttgaaaaag ctgaaaaaga atatggaaat tttgaaacgg
     1081 atcattacca agttttggga gattatcaga aaatcgtaga cgataaccgg tttctcgtga
     1141 ttgaaaggca gatcacccaa acagcagcag atagccatgt tgaaaaaaga tacgacacaa
     1201 tcgacaagaa aaacagtgtc cagctctcac ttccactttt attcaaagat gacacctatc
     1261 tatctgtttt gacaaaagaa gtcaaacggc agatggccga acaagtcaag gaagatccat
     1321 ctaagtacta ttggactgag caagatattc aagaagggac gatagaaaaa ccaacacttg
     1381 ttacgcctac acgaagtttt tatttgaaca aagagcatca attagttttg actttttctc
     1441 aatacgaaat tgctcctggt tatatgggta caccagaatt tgtcattcct aagtccgtga
     1501 caaaaacgat tttggcatcg gaagattatt tggatcatta gacgagaagt agttgcgact
     1561 agcagtttca attttcaagt aagtacaagt aaataataag aagagaaaaa gagaaagaag
     1621 agatatagtg aaaagaaatt atttagttat cagtattatt gctttatccg tgcttttgtt
     1681 tttcgaaatt acttcggtgg cctccttgat caccgaagta aaaggacaaa cacactcttc
     1741 ggagcattcg tcacaatctt tcaaacaatc agaacaatca accattaaaa gccaatcaac
     1801 tgaaacagct aacgtcaaag aagccattca agaatcagcg gaaaaggtac aaagtcagtc
     1861 atcacaatca acacaagaca gtacaaaaac gagccgagat ccggctgtca aaagcgtcgc
     1921 aatcagcttt gatgatggac caggagcgac cacaacaccg cagcttctgc gaatcttaaa
     1981 agaaaaaaac gtgcatgcca cttttttcgt cttaggagaa aatacggcgc agcatcctga
     2041 aatcgtgaag cagacagctg aagcagggca cgagattggc aatcacacat acgatcatca
     2101 gaatctggcc actctttctg cccagtcgat gacggaggaa gtgacgaaag cagacactga
     2161 gatcaaaaaa gtcactggca aaacccccac ttttgttcgt ccgccctatg gttctgtcac
     2221 gaatgtagga gcaacgatca tccagcgacc gatcatcgaa tggtctgttg attcagaaga
     2281 ttggaagaca agaaatcctg atttgatttt gcagaagatc caagcaacag tatacgatgg
     2341 ggcaattatt ttattccatg atatctatcc tgaaacgatt cgtgccgttc ctcaagtgat
     2401 cgattatctg aaagagcaag ggtatcgcat cacgacagtt ggggatttat tagggcatcc
     2461 aacggctgtc gagaactatt atggcagaaa cgatcatcgg ccagttcagt aagtgagaca
     2521 agtatcttta ataaataaca cattttaata agtagttgaa cacataacta ccttacttgc
     2581 aaaggcttaa caatatctat acaggtacaa caaaaaactg tccaccaatt ttctttggcg
     2641 gacagttttc acttaaaaat aaagaagcaa ctattcgtaa attatgctaa aatagcagta
     2701 gaacgcgtcc tgagggaatc gaacccccgt ctcaagaacc ggaatcttac gtgatatcca
     2761 ctacactaag gacgcaagca ctgggataat tgtaactaaa cagataaaaa attacaaggg
     2821 gattggacaa aaaaatgaaa tttgagcaaa atttttcaca aaaacagcag cagacacaga
     2881 aactagccat gacgcaacag ttgcaacagt ccatccaagt tttacaatat aacagtgagg
     2941 agctgttggc attcgttgag aatcaagcaa tggaaaatcc attggtggaa gttgttgagc
     3001 cggaatggca gccagattac ataaaagctt cttcatcttc ttatgaagga gaagagacaa
     3061 attacctaaa tcagattcct gatacaaaag ggtctttatt tgattcattg atcgaacaag
     3121 tccatttgaa ttaccgagat acttttttga ggaaaatcgt tctttatctt gtagaataca
     3181 tcgatttgaa cggatttttg acgattagtt tagaagaagc cggaaaagag attggagcta
     3241 cgcctatcca aatgctcgat gcgttgactc tgattcagca attagaacca gctggagtag
     3301 gtgcacgtag tttgcaagaa tgtctcatgc ttcaaacaga acgagatgat tatgcgccgg
     3361 aattagctta tatcgtgttg gaagaatgtt ttgaggaact ggtggagcga aaatggaaag
     3421 aaatcgcaca acgtttttct gttgatctac atgctgttca gcagatattc gactatttac
     3481 aaacgttgag tccaagccct ggccggattt tcgaccgtag cagcgaattg tacattattc
     3541 ctgatgtccg ggtattagtg gacgacgata aaaatgtaca agttatctca aaccgaaaaa
     3601 atcagccaaa tatacgcttt caagagagtt acttcaaaca aatgtcacaa caagcagata
     3661 aagaaactga aaattattta aaagaacgga aacaggaatt cgaatggttg aagaaaacga
     3721 tcctacaacg tggggataca attttgcggg ttgctcaagt cattgtttcc cggcaaaaag
     3781 ctttctttat cgacaaggaa cgtccaatca agcctcttac gttaaaggaa gttgcaactg
     3841 agatagatgt gcatgaatcc acggtcagtc gtgcagtcaa cgggaaatat ttggaaacag
     3901 acttcggtgt atttgaatta aaaaaatttt tcactacacg aattcctacg aatagtacag
     3961 aacagacaga agacctttca gcagatacag cgaagaaaaa gctgcaggaa ttagtcgatc
     4021 aagaagacaa aaacaagccg ttgtctgacc agaaattggt tgaattgctg aaaaaagatg
     4081 agatagcgat ctcaagacga acagtagcaa aatatcgtga tttattagga atcccgagtt
     4141 catcaaaaag aaaacgttat gataactaat cagtcaaaag actagaaaaa gtatttcact
     4201 ttttctagtc tttttagttg aaaaacgaga gcaaaactgt tagacttaca gtcgtgaggt
     4261 tgagatttcc ctttattgat gtgaaattcg aatagttaat ttcttttttt atcgtatatg
     4321 ggtcgtttta agtctacatt ggacgtaaag tgtccaacta aggagatcgc tatgctggat
     4381 gaaataaaaa tgattgaatc tgttgcacca gatattatcg aagtgttgca agaacgctat
     4441 aaaattttac gaaatatcta ttggatgcag ccgattggac gtcgaagttt atcggaaagt
     4501 atggggatca cagaacgagt attgcgaaca gaaacagatc ttttgaaaaa tctgaatttg
     4561 atcgacacct caaaaagcgg tatgacgctg acaactaagg gcgaagaagt ttatcaaagc
     4621 cttgaaaact tcatggatca gcttttaggt acgcatcaaa ccgaacaaca gttggcagaa
     4681 tattttggta accaacgctg tatcgttgtt tcaggaaaca gcgaagagca gaccaaagtg
     4741 gctgatgcat tcggcgaagc gctgagcgaa gccctagacc gactacttcc agaaggcgag
     4801 aatatcatcg ctgtgatggg aggaactact atggctgtgg tagccgaaca attgtctaat
     4861 ctggaaaata agaaacggca taacctgttt gtccctgcaa gaggcgggat tggtgaagcg
     4921 ataactgtac aggcaaattc tgtcagtgca agaatggctg ctaaagcaaa cggtaaccac
     4981 cgtgcacttt atgttccgga acaattaagt ctggcaactt acaattcttt actcaatgag
     5041 ccatctatcc aagaagtctt aaacttgatt agtgaagcga attgtgttat tcatagtatt
     5101 gggcgtgcat tacacatggc agcacgtcgt aagatgactg aaaaagaatt agtcatgctg
     5161 aaacaagcaa atgctgttgc ggaatctttt ggctactttt tcaatgaaaa aggtgaagtc
     5221 gtttataagg tccctagaat cggcatagaa ttgcaagatt tggaaaaagt cccgatcatc
     5281 atggcaatcg cgggcggaaa atcaaaagcc aaagcaattc gagcttatat gaaaaatgca
     5341 ccaaaacaaa cgtggctcat cactgatgaa gctgctgcaa atgagatttt aaaaggggta
     5401 accctttaaa ataaatattt ttttgatttt cataaggagg aaatcttaaa tgacagttaa
     5461 agtaggtatt aatggttttg gacgtattgg acgtctagca ttccgtcgta tccaagatgt
     5521 agatggaatc gaagtagtag caatcaacga cttgacagat gctaaaatgt tggcacactt
     5581 gttaaaatac gacacaactc aaggacgttt caacggaaca gttgaagttc atgaaggttc
     5641 attcaacgta aacggaaaag aagttaaagt attagctaac cgcaacccag aagaattacc
     5701 atggggcgaa ctaggcgtag atatcgttct agaatgtact ggtttcttca cttctaaatc
     5761 agcagctgaa aaacatttaa cagctggtgc taaacgcgtt gttatctctg ctcctggcgg
     5821 aaacgatgtt ccaacaatcg tttataacac aaaccacgaa acattaactg gtaaagaaac
     5881 agttatctca ggtgcttcat gtactactaa ctgtttagct cctatggcta aaacattgaa
     5941 cgacaaattt ggtgttgttg aaggattaat gacaactatc cacgcttaca caggtgacca
     6001 aatgactcta gacggacctc atcctaaagg tgacttccgc cgtgcacgcg ctgctgctgc
     6061 aaacatcgtt cctaactcaa caggtgctgc taaagctatc ggtttagtaa ttcctgaatt
     6121 gaacggtaaa ttagacggag ctgctcaacg tgttcctgta ccaacaggtt cattaacaga
     6181 attagtaaca gttcttgaaa aagaagtaac tgttgacgaa atcaatgcag caatgaaaga
     6241 agcttcaaac gaatcttacg gatacaacac agacgaaatc gtttcttcag atatcgttgg
     6301 tatgacttac ggttcattat ttgatgctac acaaactaaa gtaatgacag ttggcgacaa
     6361 acaattagtt aaaactgttg cttggtacga caacgaaatg tcatacactg cacaattagt
     6421 acgtacttta gaatacttcg ctaacttata agatagacaa acgtacaagt gtaaattata
     6481 gacatgtaca tttaaaagcg gggaagcaat cgcgcttctt ccgctttttt tgataataag
     6541 tttattcagg aggaaataaa atggctaaga aaacagtgaa agatatcgat ttaaaagaca
     6601 aaaaagttct tgtccgtgtt gacttcaacg ttcctttgaa agacggcgtg atcactgacg
     6661 acactcgtat caaagcagca ttgccaacaa tcaactacgt tctagaacaa ggcggaaaag
     6721 caatcctttt ctctcacctt ggacgtgtaa aaacagaaga agataaagaa ggaaaatctt
     6781 tagctccagt tgcaaaacgt ctaggcgaat tattaggtaa agaagtaaca ttcgtacctg
     6841 aaactcgcgg agaacaatta gaagaagcaa tccgcaacat gaatgacggc gacgttgtcg
     6901 tattcgaaaa tacacgattt gaagatattg acggtaaaaa agaaagcaaa aatgatacag
     6961 aactaggtaa atactgggct tcattaggcg atgtatttgt taacgatgct ttcggtacag
     7021 ctcaccgtgc acatgcttct aacgtaggta tcgcttcaac tggtatccca acagttgctg
     7081 gattcttgat ggaaaaagaa atcaagttca tcggtgaagc agtagaagaa ccaaaacgac
     7141 caatgattgc aatccttggt ggcgcaaaag tttctgataa aatcggcgta atcgaaaact
     7201 tgattcctaa agcagataaa atcttgatcg gtggcggaat gacttacaca ttctacgaag
     7261 caaaaggaat caagatcggt aactctctag tagaagctga taaagtggaa ctagcaaaag
     7321 aattaatcga aaaagcaggc gacaaacttg tcttgccaat cgacaatgtc tgtgcaccag
     7381 aattttcaaa tgatgttgaa acacaagtaa tcgaaggaga cattcctgat ggtttgatgg
     7441 ctttagacat cggcccagca tcagtaaaat tatttgctga tacattacaa ggtgcaaaaa
     7501 cagttgtttg gaacggacct atgggtgtat ttgaaatgag taactttgct aaaggtacaa
     7561 tcggtgtttg tgaagcaatc gctaacttag aaggcgcaac aacaatcatc ggtggcggag
     7621 actcagccgc agcagcagaa caattaggat ttgctgacaa attcacacac atctcaacag
     7681 gtggcggcgc aagcttggaa ttgcttgaag gtaaagaatt accaggacta gcagcaatca
     7741 acgataaata aacgaccaag tttgctcaat tctgacgagt aatcgatcaa aaggtgaagg
     7801 gagttttcat gactccctag ccatttttca ataaattaag gagtgtgttc ctcaatgcgt
     7861 aaacctatta tcgcaggtaa ctggaaaatg aacaaaactg catcagaagc aaaagaattt
     7921 gctgaagcag taaaaaacaa aatcccttca aatgacaaag ttgattcagt catcggatct
     7981 cctgcattat tcttacaaga attaatgaca tcagctgaag gtacagaact aaaaatctca
     8041 gcacaaaact gctactggga aaactcaggt gcgttcactg gtgaaacttc accagcagct
     8101 cttgctgatc taggtgttca ttatgtgatc atcggacatt cagaacgtcg tgaatatttc
     8161 catgaaacag acgaagacat caataaaaaa gcgaaagcta tctttgcaaa tggtatgaca
     8221 ccaatcctat gctgtggcga atctttagaa acctatgaag caggcaaaac tgctgaatgg
     8281 atcgaaggac aaattacagc tggtttagct gatctttcag ctgaacaagt gtcaaacatg
     8341 gttattgctt atgaaccaat ctgggcaatc ggtactggta aatcagctga cgcacaaatc
     8401 gctgacgaaa tttgtggagt tgtacgtcaa acagttgaaa aactttatgg aaaagaagta
     8461 tcagaagcag ttcgtatcca atacggcggt tctgtaaaac cagaaaatat tgctgaatac
     8521 atggcaaaag aaaatgttga cggtgcttta gtcggtggcg caagcttgca agcagattca
     8581 ttcttgtcat tgttagacgc tgtaaaataa ttgttgaaaa tttgcgaaaa atgcaagaaa
     8641 tgtttgcact aatgagcaaa attaactaaa atcgtatatg agggaaatcc cttaggaaaa
     8701 aacaaactca aaggagagac aaaacatgtc aattattact gatgtttacg ctcgcgagat
     8761 cttagactca cgtggtaacc caacaatcga agtagaagta tacacagaaa gcggagcttt
     8821 tggccgcgga atggttcctt caggtgcttc aactggtgaa tacgaagcag ttgaattacg
     8881 tgacggagac aaagctcgtt atggcggtaa aggtgtaact aaagctgtcg acaacgtaaa
     8941 caacatcatt gctgaagcaa tcattggcta cgatgttcgt gaccaaatgg ctatcgacaa
     9001 agctatgatc gctttagacg gaactcctaa caaaggtaaa ttaggtgcga acgctatttt
     9061 aggtgtttct atcgctgttg cacgtgcagc tgctgactac ctagaagtac ctttatacca
     9121 ctacctaggc ggattcaaca caaaagtatt gccaacacca atgatgaaca tcatcaacgg
     9181 cggatcacat gctgataact caatcgactt ccaagaattc atgatcatgc ctgttggcgc
     9241 tccaacattc aaagaagctc tacgtatggg tgctgaagta ttccacgcat tagcttcaat
     9301 cttgaaagct cgcggattag caacttctgt tggtgacgaa ggtggatttg ctccaaacct
     9361 tggttcaaac gaagaaggtt ttgaagtaat catcgaagca atcgaaaaag ctggctatgt
     9421 acctggtaaa gacgttgttc ttgctatgga tgctgcttct tcagaattct acgacaaaga
     9481 aaaaggtgtt tacgtactag ctgattcagg cgaaggcgaa aaaacaactg acgaaatgat
     9541 caaattctac gaagaattag tttctaaata tccaatcatc tcaatcgaag acggattaga
     9601 cgaaaacgac tgggacggat tcaagaaatt aactgacgta ttaggcgaca aagttcaatt
     9661 agttggtgac gacttgttcg taacaaacac tcaaaaatta tctgaaggta tcgaaaaagg
     9721 aattgctaac tcaatcctta tcaaagtgaa ccaaatcggt acattaacag aaactttcga
     9781 agctatcgaa atggctaaag aagctggcta cacagcagtt gtatctcacc gttctggtga
     9841 aacagaagat tcaacaatct ctgatatcgc tgttgcaaca aacgctggcc aaatcaaaac
     9901 tggttctcta tcacgtactg accgtattgc taaatacaac caattactac gtatcgaaga
     9961 ccaacttggt gaagttgcag aatacaaagg tttgaaatct ttctacaact taaaaaacaa
    10021 ataattcttt tctaatgaag atacatgatc gactattgaa agatagtcga gtatgatcac
    10081 ttagttaagt aatcgaactc tctacttgct gaaatatcgg ctctttgtca ataaggactg
    10141 atgagttgtg caaaatcaaa tctgagtcag aatgaaccac attctggctc a
//
LOCUS       DACDXO010000078        10154 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E23605_2017
            SAMN12639989-rid9881463.denovo.079, whole genome shotgun sequence.
ACCESSION   DACDXO010000078 DACDXO010000000
VERSION     DACDXO010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639989
            Sequence Read Archive: SRR10023389
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10154)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10154)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741914.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:42:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,972
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 33 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 17 of 73
            Pseudo Genes (multiple problems)  :: 10 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10154
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639989-rid9881463.denovo.079"
                     /isolate="E23605_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            140..460
                     /locus_tag="IWM35_002385"
     CDS             140..460
                     /locus_tag="IWM35_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9920211.1"
                     /translation="MKKRLLWCLLFLSLSLTSQPVFAEETQRSPITGTRLYKNVYVQT
                     TDLDSAIPETEERSLQPRFGYFWYCPTCDYESCWHFLHSTAAAFAFTHTQKYDGHHPV
                     IYEV"
     gene            912..1112
                     /locus_tag="IWM35_002386"
     CDS             912..1112
                     /locus_tag="IWM35_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP9920212.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            1259..4048
                     /locus_tag="IWM35_002387"
     CDS             1259..4048
                     /locus_tag="IWM35_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAP9920213.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIESGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSRHTGMDTSRFIYHVIEEMKEKPEPLDPSLEIVDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIIEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPEQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHSFFADIAHRGIGFLARNQSFYEQDFVRFLQKKIRQSNFLIVNHAFLIQETQRKEP
                     LLPASPFLLIDEVHHLPEIAEKVNDKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICMAEGLMSLVSGKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAIDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLHDFQAAMLPSTVWYPRYDRILYIGGTLKIGP
                     NRHYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLETALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAMEEI
                     YQDIHEFLKKNEEGS"
     gene            4095..4622
                     /locus_tag="IWM35_002388"
     CDS             4095..4622
                     /locus_tag="IWM35_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAP9920214.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEEADHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            4643..5833
                     /locus_tag="IWM35_002389"
     CDS             4643..5833
                     /locus_tag="IWM35_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAP9920215.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNPVIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYSLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            5873..7171
                     /gene="asnS"
                     /locus_tag="IWM35_002390"
     CDS             5873..7171
                     /gene="asnS"
                     /locus_tag="IWM35_002390"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HAP9920216.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKIAFLQLRDGTAFF
                     QGVVVKSEVSEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            8347..9021
                     /locus_tag="IWM35_002391"
     CDS             8347..9021
                     /locus_tag="IWM35_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAP9920217.1"
                     /translation="MKKRILFIGDSITDAGRDRAEATDLGKGYPLLIAGKLISQYPTM
                     KFECMNRGIGGDKIADLQVRWQEDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQTAEICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIAYGFSKYTREDGVHPTIGGHEIIAQSWIDAIAAVDFFSGDKNE
                     RKKSEK"
     gene            9018..9359
                     /locus_tag="IWM35_002392"
     CDS             9018..9359
                     /locus_tag="IWM35_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9920218.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERVDQRPSGKLIQHTLVTKENTEEIQKKFAEYLMLEAK
                     KRRIKSIFAQF"
     gene            9389..9748
                     /locus_tag="IWM35_002393"
     CDS             9389..9748
                     /locus_tag="IWM35_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HAP9920219.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     ASLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            9952..10131
                     /locus_tag="IWM35_002394"
                     /pseudo
     CDS             9952..10131
                     /locus_tag="IWM35_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308695.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
BASE COUNT         3392 a         1811 c         2174 g         2777 t
ORIGIN      
        1 aaaaagaggt tgtgacgtgg tcacgacctc tttttgacga ttatgataca aaaaacatca
       61 tttatgaaca aaatgtgaat aacgggcgtt cttgagatat gattttaatt gggaaaaact
      121 tatcgataag gaggtcttta tgaaaaaaag actgttatgg tgtttgctgt ttctatccct
      181 aagtttaaca agtcagccag tttttgctga agaaacacag cggtctccta ttacaggcac
      241 acgtctgtac aaaaatgttt atgtacagac aacagatctt gattctgcga ttcctgaaac
      301 agaagaacgg tctttgcagc caagatttgg ttacttttgg tactgtccga cctgtgatta
      361 tgaaagttgc tggcatttct tacattctac agctgcagct tttgctttta ctcatactca
      421 gaaatatgat ggacatcatc ctgttattta tgaggtatag atatgagcaa aaaagaaaaa
      481 ctaatctcgt tgatcgtatt ggtttgcctt gttcttctgg taactgtcat aggctatcag
      541 aaataccatg agaacatcag acctactaaa gaatcttccc cagcctcgac cgttgaaaca
      601 gaaccgataa gtagtcagaa agaataattt ttttagttta aagaagaaca tcagagcaaa
      661 aaaacaccaa ctgaacaata tgtcgtttat tttaaaaaaa gttcggtttt tcactttatg
      721 taatcgctga gaaaactttt tttaataaaa aaagtcgaaa aactctagct tttttgtaaa
      781 cggtatgata tactatagga gtagtttttg ttcggtaatc tgtggtagct tgtttcaaat
      841 aaacatcttc cagatataca ccattcaagt aattgcaaaa tatgtgttga tacacaagga
      901 ggatttttat tatggaaaca ggtacagtaa aatggtttaa ctcagaaaaa ggatttggat
      961 tcatcactgc agaaaacgga aacgatgtat tcgttcattt ctcagctatc caaggcgacg
     1021 gattcaaaac tttagaagaa ggccaagcag taactttcga tgttgaagaa ggccaacgcg
     1081 gacctcaagc aacaaacgtt actaaagctt aattaaccta tactaaaaaa taaacgccca
     1141 gacatttgcg tgtctgggcg tttttgtcta ttttctttaa aaagcgatga gagtattttt
     1201 ctgcactttg tagtataatt tcaaaagaac attagaggca ggagaagagg aagatatttt
     1261 gaaaccaaga acttatgcag tcgttgatat cgaaacgact gggacgaatc caaaagaaga
     1321 tcggatcatc caatttggct gcgtcatgat tgaatcaggt cggatcactt cacgtttttc
     1381 gatcgatatt catccagggc ggaagatttc aaaacaaatc cagcatttaa cagggatcac
     1441 gaatcaacgc gtccaaaaag cgccttattt cgaagatgtc gcccaaacga tttacaatct
     1501 tttggcagac accatatttg tcgcgcataa tatttatttt gattataatt ttttgaacca
     1561 tgaactcatg cgatgcggac ttcctagttt gaagatacca ggcatcgata cagtagaact
     1621 tgcacaaatt ttcttgccga cggaaccaag ctttcgctta gctgatttgt cagaaagcct
     1681 tggatttatt catgagaatc ctcatcaggc agatagtgat gccgaagtga cgggtcaact
     1741 tcttttgctg atcgaagaac ggatgagaaa attgccaatc atcacgttag agcagattgc
     1801 gcgattaagt agacacacag gtatggatac gagtcgtttc atttatcatg tgattgaaga
     1861 aatgaaagaa aaaccagagc cacttgatcc ctcattagaa atcgtcgatg ggctagcttt
     1921 gcagaaaaaa gaagtggaac tattcacatc cgtccattat ggcgagcgga catatccaag
     1981 aaaaaaacaa gcaaaagaaa aaatgtttgg taaaacactg atgtatcgaa aagaacagaa
     2041 cagactgatg aatgcggtct acgaccattt cacaaaagat gaatcaaaag atttgatgat
     2101 cgaagcagct acaggaatgg gtaaaacaat cggttatctg ctgccattga gttatttggc
     2161 tacacctgaa aaacccgctg tcatcagtac cgtttcgcta gttttgcagc agcagatcat
     2221 agaaaaagac attccattgc tgaatcaatt gctagatcag cccatacaac ctgtaattat
     2281 caaaagctat cgccactata ttgatttaca gcgttttaaa ggcacacttg tggaaccgcc
     2341 agaacaaaaa caatatgctt tgtatcaaat ggcggtctta gtttggctga cgcaaacgaa
     2401 gaccggtgat ttggatgaat tacatttaac aaatttgaac cattcattct ttgcagatat
     2461 cgcgcacaga ggtattggat ttttagcaag aaatcagtca ttttacgaac aagactttgt
     2521 tcgtttcttg caaaagaaaa tcaggcaaag taattttttg attgtcaatc atgcgttttt
     2581 aatacaagaa acacagcgaa aagaaccatt gcttccagct agtccatttt tgttgatcga
     2641 tgaagtacat catttgccgg aaatcgctga aaaagtaaat gacaaacgat taagtattaa
     2701 ctatttccat aaacaaatcc agcaattaga agaaccaaat cagctgtttg accgaataca
     2761 aagctattta ccgaaagaac atgaagcctt acgctggtta tcgatttatc gagaagaact
     2821 ttctgcgcta tgcgaagcac agatttgtat ggcagaagga ttaatgagtt tagtgagcgg
     2881 caagcaacct aacggacagt atccagaaga agtcatgatg acaaaagaga tgttggatca
     2941 gctgccactg gaatctggga cagccattga tcacctgcag cttttttatc aggagatcca
     3001 gcagctacag tcacaaatgc gtctgacttt tgagattatc caagaaagct ggacaaacca
     3061 gatgcggatc gatttagcaa gtttatatag cttatttgat caattagaca agcagtactt
     3121 gctgatggat caatggctgg aagactggcg agatcatttg atccattggt tcgttcctgg
     3181 gaacacagcg cagcagatga tttttcatct ccatgatttt caagcggcta tgttgccgtc
     3241 gactgtttgg tatcctcgat atgatcgtat tctttatatt ggaggaacgc tgaagatcgg
     3301 ccctaatcga cactatctct ctaagcggct tggcattgaa gcagcacctc ttaaagtgat
     3361 tgcctctcct tatgattatg agaaacaggc aaggatctat gtgccaaaag aggcacctgc
     3421 cgtccatcaa gtcagcaatc aagcatatat cgattattta gagacagcac ttacaggtct
     3481 gatcgaacaa gaagatcggc caatacttgt gttattcact tctcatgatg ttttgcagaa
     3541 ggtctatcag aaaatgcatc tgtctatgct agaaaaagga cgagaaatac tagctcaagg
     3601 gtttggcgga agccgggaga agcttttgaa acgcttcatt ttgtcagaaa aaagcgtatt
     3661 gtttggcgct gacagctttt gggaaggtgt agatttgcct ggggatgctt tgcagatcgt
     3721 tgtagtcaca cgtttaccct tcgataatcc gcttcgcccg atggtcaaag ctaggaacgc
     3781 ttatttagaa tcagagggga tcaatccttt ttaccaggaa tccgtaccta aagcagcttt
     3841 gaagttacgc caagcattag gtcgcttgat aaggacggag acagatcgtg gcgtattgct
     3901 tattttggat cgaagactag tcactgcaaa atatggtgca cggatcatcc atgcattacc
     3961 gaaaaaagta ccgatcaaag agctagctat ggaggaaatc tatcaggaca tccatgaatt
     4021 cttaaagaaa aatgaagaag gaagttagag gagctgtctt ttttcataat taaaatctgc
     4081 tataatgaaa aggaatgaag ataaaggagc gacgaccctt gaagaaaaaa cagacaaagg
     4141 aaaaaacgat taatcgcgtg ttacttggct tgatcgtatt tttattagcg gtcatcgtga
     4201 tcagctcgat tttttacttg cgttccagtc gtccggcggt gcaggcgaag aaagaagcag
     4261 tagaaatcgc aaagaaatat gcaaatcttg aggaagccga tcatttttat tggtttactc
     4321 gaaagaagac atacttttca ctgacaggta caaatgataa aggaaaagaa atcgccgtta
     4381 tcattcctaa atctggtgaa aaagtaaaag tattggatca aacaaaggga ctgactgaaa
     4441 acgaagcaaa acagacaatt gctgcggccc atcctgaaat ccaaatcgaa aaagctgctt
     4501 tagggatgta tgatgatcag cctgtgtggg aagtagtagg aaaagaaaca aatgggaaac
     4561 tgaattatta cctgattggc tttgaatctg gagatgaagt caagacaatc aagggaattt
     4621 aaaaggtaaa ggaatgaatg ggatggagtt atcaaaaaga acaaaacgat tagaaccgtc
     4681 tgtcactttg gcagctgcgg caaaggccaa ggcattaaaa gcaaaaggca gagatgtgct
     4741 gagtttgact gtgggagaac cggattttgc gacaccagaa aacatccaag aagcagcaat
     4801 cgaagcaatc cgcaacggga aagctagcta ttatacacca acagcaggaa tcccagagct
     4861 tcgacaggca atcgttgatt atctgaagaa aaactatgga cttgtctatg aaccttcaca
     4921 aacagtcgta acagacggag cgaaatttgc tttgtatacc cttttccaaa cgatccttga
     4981 cccgcaagat gaagtcatca ttcctgtgcc ttactgggtc agttacggtg agcaagtaaa
     5041 actggcagaa ggacttcctg tctttgtgaa aggggaagaa tccaatagct ggaaagttac
     5101 tgttgaacag ctagaacaag cacgttcaga aaaaacaaaa gccgtaatca tcaattctcc
     5161 atcgaatccg actggaatga tttatagcaa gaatgaactg caggcaatcg gtgaatgggc
     5221 agtaaaaaat gatattttga tagttgctga tgatatttat ggtcgtttag tttataatgg
     5281 caatgagttt actccaattg caacgatttc agaagcaatc aaaaatcaga ctatcatcat
     5341 caacggcgtg tctaaaacat atgcgatgac aggctggcgc attggttatg cggtgggaaa
     5401 tcctgtgatc atcaatggaa tgatcgctgt tgcttcccag tcaaccagta atccgacagc
     5461 agtcagtcaa tatgcagctg ttgaagcatt gaaaggcgag caagatacag tggaagaaat
     5521 gcggaacgcc tttgaagaac gcctgaatac gttatattcg cttgtggcag aactgccggg
     5581 tgtttctctt gaaaagccac aaggagcttt ttatcttttt cctaatgtaa aagaaacgct
     5641 acgcatgtgt aaatatgaaa atgtcacaca atgggtagaa gatcttttag aagaaacagg
     5701 ggtagcgctt gtgactggcg aaggctttgg ggcaccagaa aatgtccgca tgagttatgc
     5761 tactgatcgc gctacgttag aagaagcggt acgcagaatc aagcaattta ttgagagtaa
     5821 aagtcaaaat taatcctaaa gaaattcaag gaatataaaa ggagagtcat acgtggaaca
     5881 aattcaaatc atcgattcga aaaaccatgt gggagaaaca gtaacaatcg gcgcttgggt
     5941 cgcaaataag cgatcaagcg gaaaaatcgc atttttacaa ttaagagacg gaacagcctt
     6001 tttccaaggt gtagttgtaa aaagcgaagt gtcggaagaa gttttccaaa cagctaaaaa
     6061 tctacaccaa gaaacatccg tgttagtaac tggagaaatc cgtgaagaca gtcgttcaaa
     6121 atttggttat gaaatcggaa ttacaaatgt ggaagtggta agcgagagcc acgattatcc
     6181 gatcacacca aaagaacacg gaacagactt cttgatggat catcgtcatt tatggctgcg
     6241 ttcttcaaga caacatgcga ttatgcagat cagaaatgaa atcattcgtg caacgtatga
     6301 attctttaat caacatcatt ttgtcaaaat tgatccgccg atcttgactg gttctgcacc
     6361 agaaggcact acggatctat tcgaaacaaa ttactttgat caaaaagctt atttatctca
     6421 aagtggccaa ttatacatgg aagctgctgc tatggctttc ggaaaagtct tttcattcgg
     6481 tccgactttc cgtgctgaaa aatcaaaaac acgacgtcat ttgatcgaat tctggatgat
     6541 cgaaccagaa atggctttca ctcatcaaga agaaagttta gaagtacagg aacaatatgt
     6601 agcattctta gtacaaaatg ttttagatca ttgcgaatat gcattagatg tactaggacg
     6661 agacaaagaa gtcttaaaaa cttatacaaa attgccattc cctcgtatta cttacgatga
     6721 tgcaatcgaa ttactgaaga aaaatggatt tgatgacatc gaatggggcg aagactttgg
     6781 ttcacctcat gaaacgttca ttgcgaattc attcgaacag cctgtcttta tcctaaacta
     6841 tccaaaagcc atcaagccat tctacatgaa accacatcca acaagagaag acgtggtaat
     6901 ttgtgcagat atgatcgcac cagaaggtta cggtgaaatt attggcggaa gcgaacgggc
     6961 aacagactat gatttcttgc tagaacaaat ccgtaatcac ggtttggatg aaaaagaata
     7021 ttcatggtat cttgatctac gtaaatacgg ttctgttccg cactctggtt ttggtctagg
     7081 ccttgaacga acagtcactt ggattgctgg tatcgaccat gtacgtgaag ctattccatt
     7141 cccacgtttg ttgaaccgaa tttatccata agaggtgatg aaaaagctgt tctgcatcaa
     7201 gtaattagaa cagccaagca aaaacagctg ttcatgtttt tgaattggcg ggacttatta
     7261 ccgcagatgt aagcttttga ataccgttta gtggagaggt tgtgaaaaag ctgtttagtt
     7321 tcgagtatta agaaaaattt tgtggaaatg gctttccaca tttttgcata aatttggctt
     7381 aataccgaga aactggcttt tgaacaccgt ttaaggaaga ggttgtgaaa tcagtgcctt
     7441 gacctgagta ttagagaaac aaaacggaaa aatagcttct catattttac cgttttttta
     7501 gctaatatcg agggtcagct gattgaacac cgtttatatc aagaggttgt gcgcctgccg
     7561 ttcagctccg agtcacaagg agtaatttca aaaaacagtt tttcatgttt gatgactatt
     7621 gtgacttgtg accgaggagt tggccggaga gcaacgttta tttaaaaaaa agaactgcga
     7681 actctcgcag ttcttttttt aaaatacggg ataaaggaag tttccagcag acaagagaag
     7741 aaagcaaaag aaattgctat ttgattcaca aggttctata acgaaatcgc aatcaatcaa
     7801 acatatacaa agaaggttat tcaaatggaa atctgatttg atgatgcttc tgctgttgaa
     7861 ataaagcagc atctagtacc tgaaaacaaa ctaatagtaa cattcgaaga tggagtaggt
     7921 ccgtattttc agcatgcaat gatccatatg taagtttaat tcatccttga aatcatcgga
     7981 ccagataaag ttatgaccaa tctatcccaa gcaatctaga agagatatta gtaaaagaac
     8041 atttactgga agtcttagat gagcctataa atatatgttc tgatggcgtc atcagtggtc
     8101 tgattgacgg aagcttaggt gtgatcgatt tctctggacc aaacaacgaa ggaatcaagg
     8161 ggaatccggc acgttaagaa aaaaagtata aaaaactaga cacttgatga gcgttcattc
     8221 aattgattga agggactagg ctggtgtcat tttttataca tatattttct ctttcgtctt
     8281 gatagggctt atgggataat ataaggaaga aaatcatcgt tttattcaaa aaggagtttt
     8341 tgacaaatga aaaaacgtat attatttatc ggtgacagca ttacagacgc aggaagagac
     8401 cgagcagaag caactgatct aggtaaaggt tatccacttt tgattgccgg aaaactaatc
     8461 tcccaatatc caacgatgaa atttgaatgc atgaaccgtg ggataggagg agacaaaatt
     8521 gcagatctgc aagtacgctg gcaagaagat tgtttagctt tacaacctga ctatgtatcg
     8581 attttgatcg gaatcaatga tacctggcat aatactagcg atcaggctat attcggaact
     8641 gagaaatctg cgcagcaatt tgaatccgtt tatcgaaaat tactaaatga acttcaaaca
     8701 gcggaaatct gcaacatcat cttgatggag ccgtttgtac ttcctataac cgacgaccgt
     8761 catggctggc gtaaagactt agaccctaaa atccaaattg ttcgtaaact tgctaaggaa
     8821 tatggcgcat tgctggttcc tttagatggc atattcaatg ctttagggat tgcttatggt
     8881 ttttcgaaat atacaagaga agatggggtc cacccaacga ttggcggaca tgagatcatt
     8941 gcacaaagct ggatagatgc aattgccgca gtcgattttt ttagtggaga taaaaatgag
     9001 cgaaagaaaa gtgagaaatg atggaaatac ggtgggcagt aaaagaagat ttacctcaaa
     9061 tcaatcagct gattactgat catccagaat cattcgtaaa agcaaaaacg gcagctgacc
     9121 taaatgataa gaataaacta ttgctcgttg ctgtaacaaa agaaaaacgg attatcggat
     9181 ttgcgcaagc ggacgtgcaa cagtcagaag aacgggtgga tcagcgacca tcaggaaaac
     9241 taatccaaca cacattagta acaaaagaaa atacggaaga aatccaaaaa aaatttgcag
     9301 aatacttgat gctggaagct aaaaaaagaa gaataaaatc aatttttgcc cagttttaat
     9361 caaaaacagt aatagaagga gaaatacaat gagttgttta ggaaatgtca tctggtttat
     9421 attcggcgga ctaatcggtg gtcttagctg gttagcagcg gggatacttt ggtgtatcac
     9481 gatcataggt atcccaatcg gtatgcaatg ttttaagatc gcctcccttt cttttgcgcc
     9541 ttttggcaaa gaagtaattt atcaccagtc tggcatgtca ttgatcgtta atatcatctg
     9601 gctgatcgtt tcaggcctac caattgctct cgcacatgtc gtcagcggca tccttctatg
     9661 tatcacgatc atagggatac cttttgccaa gcaatctttc aaacttgctc gattggcgtt
     9721 gatgccattc ggtgcagaaa ttgtataaaa agagaataat aaaggttaga aaacgcatag
     9781 agtcaggtgg gatacatgta tttcacctga ctctatgtgt tttcctgtat aagttctccc
     9841 atgtgacttt tacgtctttt atttatatgg gattactgtt tatggaaagt tgaaataatt
     9901 ttcattaaag aactctttct atatttttga aagcgtagtg gagcttcgaa tatctgggaa
     9961 ggacattttg cagggattga aaaaagatat tgtcctatcg aaaaattaat gaatttagac
    10021 aaattgccag caacaggctt caaacttagt tgctatcctg tcaagatcaa gaaggcaagt
    10081 gcgggttgga taagagcagg ggcaatgatc gaagagaaaa agaaagaata agccaaaaca
    10141 tctgtatgga agaa
//
LOCUS       DACDXO010000079        10086 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E23605_2017
            SAMN12639989-rid9881463.denovo.080, whole genome shotgun sequence.
ACCESSION   DACDXO010000079 DACDXO010000000
VERSION     DACDXO010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639989
            Sequence Read Archive: SRR10023389
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741914.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:42:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,972
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 33 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 17 of 73
            Pseudo Genes (multiple problems)  :: 10 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10086
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639989-rid9881463.denovo.080"
                     /isolate="E23605_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(247..759)
                     /locus_tag="IWM35_002395"
     CDS             complement(247..759)
                     /locus_tag="IWM35_002395"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HAP9920220.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(773..3070)
                     /gene="recJ"
                     /locus_tag="IWM35_002396"
     CDS             complement(773..3070)
                     /gene="recJ"
                     /locus_tag="IWM35_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HAP9920221.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(3212..3646)
                     /locus_tag="IWM35_002397"
     CDS             complement(3212..3646)
                     /locus_tag="IWM35_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1049 domain-containing protein"
                     /protein_id="HAP9920222.1"
                     /translation="MKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPLILV
                     IIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQREFRN
                     ERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(3656..4444)
                     /locus_tag="IWM35_002398"
     CDS             complement(3656..4444)
                     /locus_tag="IWM35_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP9920223.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(4441..5382)
                     /locus_tag="IWM35_002399"
     CDS             complement(4441..5382)
                     /locus_tag="IWM35_002399"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HAP9920224.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(5559..6146)
                     /locus_tag="IWM35_002400"
     CDS             complement(5559..6146)
                     /locus_tag="IWM35_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HAP9920225.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(6367..7680)
                     /gene="obgE"
                     /locus_tag="IWM35_002401"
     CDS             complement(6367..7680)
                     /gene="obgE"
                     /locus_tag="IWM35_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003126661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HAP9920226.1"
                     /translation="MSMFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGG
                     DVILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAET
                     GALLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVL
                     ADVGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLE
                     RPQIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNAT
                     ADLLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTN
                     FDHDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            7968..8978
                     /gene="gap"
                     /locus_tag="IWM35_002402"
     CDS             7968..8978
                     /gene="gap"
                     /locus_tag="IWM35_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HAP9920227.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(9063..9998)
                     /locus_tag="IWM35_002403"
     CDS             complement(9063..9998)
                     /locus_tag="IWM35_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP9920228.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
BASE COUNT         2778 a         2298 c         1746 g         3264 t
ORIGIN      
        1 ttttacgggg gtgtgacaaa aatcggtcac atcctttttt tcgaataaac ggtgttctaa
       61 agccagtttt tcggtattaa gtcaaattgc ggaaaaagtg attttttaaa cttctactgg
      121 tctttgcttg tttccattta taaaaaaata gcgaccctca aaagtcagat agagaatctg
      181 acttttgagg gtcgctttaa aatcgcttct ttaagaataa catcaagaat agcttctttt
      241 ttctgcttaa tattccatta atgtaatgat gtcatatcct ttgattttgt cgcgtccatg
      301 gagatccatc aattcgatca aaaatgcgca gcctacgact acaccgccta attgttcgat
      361 cagttcgatc gtcgctttga ttgttcctcc agtagctagc aaatcgtcac aaatcagcac
      421 acgttgacct ggtttaatgg catccttatg caatgtcaat gtatctgttc catattctaa
      481 tccataagtc acttcgatgg tttctcgagg taacttccct tttttgcgga ctggggcaaa
      541 accgactcct aactcataag caacaggaca tcctacgatg aatccacggg cttctggccc
      601 aactaccata tcaattcgtt tttcttttgc ataatttacg atttgtttag tagcttcccg
      661 ataagcgtca ccatctgcca tcagtgggga aatatctcga aacacaattc cttttgacgg
      721 atagtcggga atactggcaa tataatcttt taaatccact ctttgtttcc tcctaggttg
      781 ttaaccattt ctttatggta ggaatatcgc tcaaaagcaa aaattcctca actttgattt
      841 tattgagccg tcgctggtat aatctgctct cagtcaaagg atgactttct gggctagcga
      901 ctttttgtaa aacaccgttc tctattgtaa caaatttcaa ttcaaaaaac acctgtatca
      961 taaaaattaa taatttttca gggatacgca aatattgagc tattgctttt attttatgac
     1021 gtatatcaat tcgttcttga gaatggatca atttaaacaa ttttgcgtac tgttctcttg
     1081 tgcctacccc attcaaatag gcctcatcag gtgaaatccc catcagataa acacgattga
     1141 atgaataaga ccgaaaaact tctttcagat ccgctaaatc atctggacaa tccataacaa
     1201 ctagagagtt tggttttctt ttttcaatta ttgcctttac agaatctagt ccttcgaaaa
     1261 caatcgcagc ttcctggata atctcaggca cgaatttcaa cgtcgaagaa tcaaatgcta
     1321 aataaaggct accttccgac gttcggctct gttcacggaa acgttttgcc cgccaatcaa
     1381 atacttgcag accacccaca gaaaaatcag ataccatcag ctgcggtttc ttacgcccgt
     1441 tccattcatt gatcgaaagc tttcctacca catctacctg attattcagc agttcttctt
     1501 cttgagtacc aaaaccaaaa gcgaccgcat caagctgact cgatgcatcg gaaaggacaa
     1561 atttcagatg ctgttggttt gctccgattc ttttgatatt ttctgcttgg acttgtcgaa
     1621 ataaaaactg aggcaaagga ttatccgtac caaatggcgc caatagtttc agctcatcaa
     1681 ttcgctctac tgttacttca tttggcagca atacttcatc aattctcaac tcaggccctc
     1741 tcatcaagtc aatttgatga tcgactatat attggttcat cttctcctgt agtatagtga
     1801 cattttcact tggcatcgtt aaaccgactg ctgcatgatg tcctccgaag taagtaaata
     1861 aatctcgcat ctggtcaagc atttcaaaaa gattcaaggc ttcaatacta cgtccagatc
     1921 ctttagctga tccatcttct tttaaagtaa ggacaagcgt tggtttaccc gtttcattca
     1981 ttattttccc agcaacaatg cccaaaacgc cttcatgcca tcctcggttt accaatagat
     2041 ggatatgatt ctccttattg accatcgcta atgcttcttg tgttatctgc tctacgattg
     2101 cttttctttc ttcattgatc tcattcaatt tttttgcttg aacggatgct tcctcttcat
     2161 caaacgtcgc aagtaacgag acggcaggac gtggatcgcc catccgaccg attgcattta
     2221 gtctgggagc aatcgaaaaa ccgatcgttg tttcgtctgc gtccctcatt tttacgccgc
     2281 tttcttcaaa tagcgcctga agtcctatcc gttctgaatg atgaatggca tcgattccta
     2341 atgaaacaag gattcgattc tcatcagtca atgaaactaa atcggcgatc gttcctatcg
     2401 ctactaaatc caaaaattct gcaggcggtt cttccaataa tgcagaagct actttgaatg
     2461 ctactccgac tcctgccaaa tctggaaatg gatagtgccc cgctggatga cgagggtgaa
     2521 caatcgcata agcgtctggt aaattctcag gtagttcgtg atgatctgta atgatcacgt
     2581 ctacaccagc ttgctgagcg tatgctaccg catcatttcc tgctaccccg ttatcaacag
     2641 tgatgatcaa ctggattcct tcttcgatct tttcttggta taccgattgg tttggtccat
     2701 acccatgctc aaatcgattt ggcaggaata cttctacttc tgcaccaagt aattccagtg
     2761 tttctttcat taccgttgca ctggtgatcc catctgcgtc ataatcaccg taaacgagga
     2821 ttttttctcc actgattaca gcttcttgga ttcggctgat tgctttatcc atatcgtgta
     2881 tcagatacgg atcatgtaat tcttgttcct ttggatgtaa aaatcggttg atatcctttt
     2941 cctcatggta acctctttgc cacagtaact taccgacaaa tggagataat ttcagttcac
     3001 ggatcatttg gagaaaggat tcagaaggct ctgtctcgct taacttccaa tcaaaatttg
     3061 cttgtctcac tttctcactc ctaacttagt tattatagca aaggaaatca gcaaaaaaaa
     3121 gcagaaagaa agaataaaaa cagatcagat ccgctagaca gatgttcagc aggacctgat
     3181 cttttgtacg accatttatg aaatatccct attagtcaaa ataattgaag ctttgtttgg
     3241 gcgattgagt tttttgttct tccggcgctt tcaattgact gatttgtgcg gttttcgctt
     3301 gtaactccgc ctgataagca gcttccaact ccgcacgttc gtttctaaac tcgcgttgct
     3361 gttcttcttt tgcttcttca attttctttt gaagttctgt ttgatattct gacatttcct
     3421 gagtcagctg tttgatttgc tttttttgct gccatagtgt tgccgaagca gtcaaaaata
     3481 caatcagtgc accaatcagc gcagaaccga tgatcactaa gatcaatggc gcagaaaagg
     3541 aagtgaagcc aaaacttaca ggaacatttt tattgttcaa tacagcaaaa agaacaataa
     3601 tcaaaactaa aacaaatcca agaatcacat tgctttgctt tttcaattcc ttcacctact
     3661 ttttgttaaa aattccgctg gctaaataat caccaatcgt tggaaacagc gtataaaatt
     3721 ttgatgcaac ttctaaaata aacggttgat tgatttcccg ttttggatgt ctcatattgc
     3781 ggacgatcat ttttgccaac ttggcaggtt ctaaaacgat ttgtccgact ttttccaaat
     3841 agcttccgct tggatcagcc ttatcgaaga aatttgtctc gattggacct ggattcactg
     3901 tcgtaacagc tacgccaagt ggtttcaatt ctaatcgcaa agcgttggag aagccaagga
     3961 ctgcaaattt cgtggcagaa taaaccgttg attttgcagt tgccattttt ccagccatag
     4021 aggcgatatt aatgatatga ccttgtcttc tttctgccat atcgatcgca aatttttgtg
     4081 tcaaaaccat cattcctaag atgttgactt caaacatatc gtatgctttt ccaaggtcga
     4141 atgtaaggaa gtcttcgaaa atcccaaaac ccgcattgtt taccagaata tcgacgcgcc
     4201 cgacttcttc catcaccttt tcatataatt cgtccacgct ttcaggattg ctgacgtcta
     4261 ccttgaaagc ataagcctct ttgccgctca agcgtctgca ctcgtctctg acaccttcta
     4321 caaatgccat tcttcgtgcg caacttataa cgattgctcc ttgctttgct gcttcataac
     4381 agatttgtgc gcccagaccg cctgaactac ctgttactaa aacaacttta tcttctaaat
     4441 tcatgcctct tcctcctcgc gaaatggaat ctcaatgcta tcaaaatctt tcataatcgt
     4501 cgtgttttca aacacttctt gtgcttcttt ttcaagctct aaagctgctt ttcctaaata
     4561 tctcgcactg atatgagtaa gtagcagccg ttttgcctgc gcttcttttg caacttcagc
     4621 agcttgatga gtcgtggaat ggaaatagtt gtgcgccatt cttgcttcat ctttattaaa
     4681 tgtgctttca tgtaccagaa tatcgctatt ttccgcaagc acaacggaat tatgtgtttt
     4741 tctcgtatcg cctaaaatag tcacgattcg tcctttttta tctggaccta cgaaatcttt
     4801 tccattgatc gtccgcccat cttctagttg gatcgtttca ccttgcttca gtttgccgta
     4861 cagtggtcca gctggaatac ctaattcttt tagacgatcc acctgcaatt ctcctttatg
     4921 atcatgttca accacacgat aaccaaaact gtcgatccca tgatttagtt tttttgtata
     4981 aacagaaaat tgctgatcag taaaaatcgg ggctgtttca tttagctcaa tgaatttcag
     5041 cggataactt aaacgcgttt gcgaaatacc taaagagact tttatgtatt cctctatccc
     5101 ttttggcccg taaatctcta atggcgtatc cccaccttga aatgaccggc tgctgatgag
     5161 cccgggaagt ccaaaaatat gatcgccgtg taaatgtgta atgaaaattt taccgatttt
     5221 tctcggacga atagtcgttc gcaaaatctg catttgcgtg ccttcgccac agtcgaacaa
     5281 ccaaacttca ttgcgctcgt ctaaaagttt caatgctata cttgtcacat ttctatgttt
     5341 tgcaggaaca cctgcacctg ttcctaaaaa ttgtaattcc ataatctacc tgactttctt
     5401 taagttcctc tttaatttta gggtaaaaag aagggataat caaccgtaag tcgagaatgg
     5461 gttcaaatta aaaataacgt tctaaagcga tagcgttttc tttgaaaatt actataaaaa
     5521 cagcagcgaa cccgtttgag atcctctgct gttttttatt aatttgaaga tccagaactt
     5581 tcattgtcgt tggaagtttg cggatgtttg tccttccaag tcttttgtgc agcttctttc
     5641 gcatgatttt cagcttcttt cgcatcatgc acatcttttt cgacatcttc ataatccaag
     5701 cgagtaattt ctccgcctaa ttccatcatt accaattgat tcagtgggcg attgtcctct
     5761 tcatcagcaa tcaagatcag tcctgtagct ccttcaccga ttttatttcc tacaaagtcg
     5821 aacacggtct gggcatcttt tatttctttg gcatcttgtg tcgcaccgat cattcctccg
     5881 gcaaaccatc ctaataaaat ccctagcgga ccacctaata caccaaccaa cattccgatt
     5941 aatccacccg tagaagattt gttattaccg gtaaagtcga taaaatcttc tattttgaat
     6001 tgatgtgtcc cattaggatc atggctgaca actgccatct gttctccttt aatcgaacga
     6061 acagcatgta actttttgat ttctgaaaat gcctgatatg cctggctttc tacttgaaaa
     6121 tttaaaatga ttactcgttt gctcattaaa aacgcccctt tcataccttc ttgttattta
     6181 ttttaccttt aattgaaaaa taagaaaaga aaagcactct ttttttccaa taaaataggg
     6241 ctgtgacaaa agtcgtatca cagctccttt ttccgaataa acggcgttct tgaagctgat
     6301 cacttctgtc acaacctccc ttaaatctga atctatattt tttggtattt gtttttattt
     6361 gcttgcctat tcgacaaatt caaattcaaa ctcaccgata cgtacaaggt cgccatcttt
     6421 cgcaccgcgt gcccgaagag cttcatccac acccatacca cgtaattgac gagcaaaacg
     6481 catcactgtt tcatcatgat cgaaattcgt catttggaat aatttttcga tcttctcacc
     6541 agataagatc cacgttgcat ctgaatcacg atcgatttgg aactcaggac cttctggatt
     6601 gaatccatag tgtacagttt cttcttccag ttcttcttcg tacaatggga attctggcgt
     6661 tacttctaac aaatctgctg tagcattcaa taacgcgtcc aatccttgtc gagtcactcc
     6721 agaaattgga aatactggga tgtcatcagc aaattcatct tctttttcct tgttcagctg
     6781 ttctttgaat ttcactagat tttccggtgc atccggcata tccattttgt ttgccacgat
     6841 gatttgcgga cgctctagca agcgtaagtt ataagtagat aattctttgt tgatggcaag
     6901 atagtcttca taaggatctc gtccttccat cccactcata tcgatcacat gtaaaattac
     6961 tcgtgttctt tcgatatgac gaaggaattg tgtacccagc ccaacacctt gcgaagctcc
     7021 ttcaatcaag cctggcaagt ctgctgcggc aaaacttctg ccatcacttg tagtaaccat
     7081 tcctagatta ggtacgagcg tcgtgaaatg ataagcgccg atttttggac gagcagatga
     7141 aataacggat agtaaagtcg atttcccgac agatggaaaa cctacaagtc caacgtctgc
     7201 caatactttc aattctagtt cgatttttcg ctcttgtcct ggttcaccgt tttcagcaat
     7261 ttccggtgca ggattccgag gagaggcaaa acggatattc cctcgtccac cgcgtccacc
     7321 ttttgccaca actagtgttt gaccattttc aattaaatct cctaaaagag caccagtctc
     7381 cgcatctcgg acagtggttc cttgtggtac tttaacatag gtatcttctg aaccacgacc
     7441 gtgcatccct ttactcatcc cattttcacc tggctgcgct ttgaaatggc gattgaaccg
     7501 aaaatccatc aatgtacgta acccttcatc gacgatcaag atgacatcgc cgccacgacc
     7561 accgtctccg ccagccggtc cgccgtcagg aacgtatttt tcgcgacgaa atgcaaccat
     7621 tccgtctccg cccttgccag ctttcacatc gatcgttact tgatctaaaa acatggacat
     7681 aattaagtcc tcctattatt catttattta tttgactgcc tgaatttcca caataaacaa
     7741 aagtcttact tattttatcg gtgaaatctg attttgtcta tcatttcagc aaaaagattg
     7801 tttaccttct tcttttttta tcatttggta aggtttccaa ccttttttct gctaccaagt
     7861 ataatttttt gacaaaatat tctttgcgta gcagaataac agtgtattaa ttgtattaat
     7921 tgaggatgcc gtttcacagc acccctaata aaggaggaac ttttgatatg acagtaaaag
     7981 taggtattaa cggatttggg cgtattggcc gtctggcttt ccgccgcatc aaagaagtct
     8041 cagatgatat tgaagtagta gcaatcaacg acttaacaag tccaactatg ttagcacaat
     8101 tattgcaatt tgactctact catggtactt atcctggcac agttactgca acagaagact
     8161 caatcgtagt ggatggcgaa gcaactcgtg tttatgcaga acccgacgca agcaaaatcc
     8221 cttgggcaaa agaaaacggt gtagatatcg tactagaatg tactggtttt tatacatctg
     8281 aagaaaaagc aaaagcgcat ttagatgctg gtgtcaaacg agtagttatt tctgccccag
     8341 ctggtgctat gaaaaccatc gtctataacg tcaatgatga tacattagat gaaaacgaca
     8401 aaatcatctc agcaggttca tgtacaacaa actgtttagc acctatggct tatttcttaa
     8461 ataatgaatt tgggatcgaa gttggtacaa tgacaacagt tcatgcctac acttccacac
     8521 aaatgctctt agatggacct gtaaaaggcg gtaatttgcg tgctgctcgt tcagctgctg
     8581 acaatactat cccacattct actggtgctg cgaaagcaat cggcttagtt atccctgagc
     8641 tacaaggaaa attacaagga cacgcacaac gtgttccagt cgttgatggt tcattaactg
     8701 aattggtttc agtattgaaa acaaaagtaa ctgctgatca agtaaacgaa gcaatcaaaa
     8761 aacacacagt tgacaatcct tcattcgggt atgatgaccg tcaaatcgtt tctagcgatg
     8821 tcattggaac aactcaaggt tcgatctttg acccaacgca aacagaagta acaactgctg
     8881 gtgatttcca acttgttaaa actgttgcat ggtatgacaa tgagtatgga tttacttgcc
     8941 aaatgattcg cttactagaa aaattcgcta acttgtaatt tgatagatca atcgataaaa
     9001 gaagcctttg actggatcca gtcaaaggct tctttctttt catcccctac ttttaaactc
     9061 gttcaatgaa catattggct taaaaattgt tggactactt gctgatattt ttcaggatct
     9121 gtcttgtatg cttccgcatg ctcggctcct tttacaacat acttttcttt tggtcccttc
     9181 gttgcacgat agaccttatc taacatttca aatggtacaa atgtatcttc gtctccatgt
     9241 ataaagagca ttgggcgctg gtttttcttc aattgttcca ctgcatttgc ttcaccgaaa
     9301 aagtagccag cacggacttt tgtcactaga ctcgttactt gcataagcgg aaatgctgga
     9361 agactaaaca tatcgtctag ctgataagca agctcatcat gaacggaaga ataaccgcaa
     9421 tcttcaatga ttgctttcac attatctggt agtttttctc cgctagtcat cataactgtt
     9481 gctgctccca tgctgacacc gtatagaacg atttcttgcg attttccatt gttttctagc
     9541 actttgttta tccactgtac atagtcttta cgctctggcc atccaaatcc aatgtaatcc
     9601 ccttcgcttt ttccatgtcc tcttgcatca ggaacaagga cattgtaacc catatcatga
     9661 aacatctttg cgtaattggt catcgtttca gcgtttccca tatagccatg agccacaaga
     9721 gctgtttttt cacttttcgt ttcagcagga agataaatcg ctgataattt cagaccgtct
     9781 tttgaagtga tggtccaatc gcttcggttt tcttttgaac taaaccattt atgtgcatcc
     9841 gtttctttgg atgtctgtgt tgtaccaggt gtatcttcgt ctaaaaagtc tttctcagaa
     9901 ggcacaacag cataattata aaaataattc cctgcaaaca ctaacgcaac aatcagagct
     9961 aaagcgacgc ctaaaaggat tttcatccaa agtttcataa atcatccatc tcatttctct
    10021 tttttcatat aaccttgaat tagtataatc aaagaatctc tactatgcaa tgttcatttt
    10081 tataac
//
LOCUS       DACDXO010000080         9986 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E23605_2017
            SAMN12639989-rid9881463.denovo.081, whole genome shotgun sequence.
ACCESSION   DACDXO010000080 DACDXO010000000
VERSION     DACDXO010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639989
            Sequence Read Archive: SRR10023389
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9986)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741914.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:42:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,972
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 33 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 17 of 73
            Pseudo Genes (multiple problems)  :: 10 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9986
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639989-rid9881463.denovo.081"
                     /isolate="E23605_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(11..2131)
                     /locus_tag="IWM35_002404"
     CDS             complement(11..2131)
                     /locus_tag="IWM35_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAP9920229.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLAKGKNVIYIHLESFQQFLIDYKLTDENG
                     LQHEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYTSAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHNG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            2361..3539
                     /locus_tag="IWM35_002405"
     CDS             2361..3539
                     /locus_tag="IWM35_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HAP9920230.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIVGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            3555..4313
                     /gene="aroD"
                     /locus_tag="IWM35_002406"
     CDS             3555..4313
                     /gene="aroD"
                     /locus_tag="IWM35_002406"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HAP9920231.1"
                     /translation="MVWVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFVSMPIITMSMGNLGKISRLAGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            4336..4821
                     /gene="ybaK"
                     /locus_tag="IWM35_002407"
     CDS             4336..4821
                     /gene="ybaK"
                     /locus_tag="IWM35_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HAP9920232.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSRIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(4892..6145)
                     /locus_tag="IWM35_002408"
     CDS             complement(4892..6145)
                     /locus_tag="IWM35_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP9920233.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            6263..7612
                     /gene="gdhA"
                     /locus_tag="IWM35_002409"
     CDS             6263..7612
                     /gene="gdhA"
                     /locus_tag="IWM35_002409"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HAP9920234.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNVQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            7724..9070
                     /locus_tag="IWM35_002410"
     CDS             7724..9070
                     /locus_tag="IWM35_002410"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HAP9920235.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(9378..9764)
                     /locus_tag="IWM35_002411"
     CDS             complement(9378..9764)
                     /locus_tag="IWM35_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAP9920236.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYPEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
     gene            complement(9813..>9986)
                     /locus_tag="IWM35_002412"
     CDS             complement(9813..>9986)
                     /locus_tag="IWM35_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HAP9920237.1"
                     /translation="TVETVFSSLEKLGCQNFNSRSVKGLESRFESILLAYSVLLSRAQ
                     RRFEGTLRYSLGY"
BASE COUNT         3190 a         1814 c         2038 g         2944 t
ORIGIN      
        1 atttattttg tcattcggaa gaacttgtgg tcgttccgtg aagttcttgg tacgtttgcg
       61 ttttataaag atcaacagtt gattgtccgt tgtgttcact aaagacacta gttgacttgt
      121 caccaagaga tttttcagct tcttttaatt tctctaagcc attcttgtag tcataatcac
      181 tggtatcaat tgggtctaat ccactattcg tatagaaccg aagaagatca ccattattga
      241 tttggtcaga aacagctaac tgtttatcaa ctttttcttt cagtgaatca atttcattct
      301 gtacttcctc agtaggttca gtaatagctt caccagtttc gttactatag atcgtaccac
      361 cgtagtaagt atattttggt gtaacaaaat caccatcacg gaaagaaaca agctgttcat
      421 gatctttcga gaatagatct tgtcctaact gaatataatt ttttgtatct acacctagta
      481 agtgaagtaa tgtaggcaag gcatcaactt gtccgccgta agtgtgattg attccgccct
      541 gtgtttgtcc agggatatgg atcatataag ggacacgctg catttgtgca ttgtcgtagt
      601 ttgtccaagt agaacttgtt tttccaacta attcagctag atttttattt cttgaattcg
      661 agacaccgta atggtcaccg taaaggacga tgactgaatt atcatacaag ccgctttctt
      721 tcaaataatt gaagaattct tgtacggcgg tatctaaata attggctgtc gcaaagtaac
      781 cgttgatcgt ttcatcattt gtattggcta acgggaatcc tgcttcgtca ttggtgaatt
      841 ttgaataagg ataatggtta gatacagcaa tgaattttga ataaaatggt tgttgcagac
      901 gttccaagta ttgtacggat tgttggaaaa atggtttatc atgcaacccg tattggaacg
      961 aattgtcatc attaacatca taataagaag aatcaaagaa ataatcatat cctaaattct
     1021 tgtatgtctc gtttcgattc cagaatgtac cagcgttccc atggaatacc gcactagtgt
     1081 aaccttgtgt ttgacttaaa atatcaggtg cagcttggaa agtatttttt ccgcctaatt
     1141 gtgtgaacaa ggagccttga ttcaagccaa acaatgaatt ttccatcagc gtttccgcat
     1201 cgcttgtttt tcctgctttt acttgatgga aaaagttgtc aaaactgaac gtgctgttgc
     1261 tgtggtaaag actattgatg aaaggtgtaa cttcatgctg gagaccattc tcatctgtca
     1321 atttataatc gatcaggaat tgctggaagc tttctaaatg gatataaatc acattttttc
     1381 ctttagcgag cccgaacaaa tcgtcatttg gtgctgcata atgttctttt acataatttt
     1441 ctacttcttt catatcattg ggacttgctt ctgcccgcac ttggtttgtc tggtaagttt
     1501 gaacaccgtc ataaaccatg aaagcattca agccaagata tttgaccaaa taatcacgag
     1561 agaatgttcg agtcagcaat tctggacgat cagcttccgc cataaaaaga tttcctgaga
     1621 aaatcataac tgctaatgta gagatcgcaa aagccattct tgctcggatc gggcgagggt
     1681 ccaacttgat tttttttgca atcagcagaa cagtcaagat aataaaatcg atccaataga
     1741 tcacatcata tgggcgaaac agtcgaatgg cactttctcc cagaccactc gctactttcc
     1801 cagcacctaa aatcgtgttg atcgtaatga agtccgtgaa ttctcgataa taagtgacat
     1861 tcgaaaaaac aagcaatgtc aaaagaaaat aaatgaccat catcgtgata taagcaactc
     1921 tagggcgtct gatataaaga gcaattccaa gtaataacaa tgtagttgca atagggttga
     1981 taaacaagat aaagtattga atgccacttt cgattccgag atggaaatca actaaatagg
     2041 caaaaatcgt tttcaaccaa agtaaaacga ctagtaaaga aaaaaatccg agcctcttat
     2101 tcaaaaaggc aggtgttttt atacatttca agataatctt cctttctgca cttcttaatc
     2161 acagaagtgt catgaaacca tgttacacag ctcacttatt ctcatttatt ttactagtat
     2221 gacaatatag tgtcaattga aaccagaagt ttatgctgtt tcattaaggt tttctttaag
     2281 cttcatgaaa aaatgagcag taagacaagt cctatgtttt ttgttatact tgggaagaga
     2341 aagctaaagc aggtgaagga atgaaagtaa aagtaaaaaa aagagcaagc aatcgattga
     2401 aaaaaggata tccactgatc caaaaagaag accttgctca tccccaaaaa gaaaccacgc
     2461 aatgggtgga attagttgat tcgcaaaacc aatttatcgc aaaaggatat ctaggaaagc
     2521 agaataaggg aatcggttgg gtattgagcc agcagaacat cccatttgat caatcttttt
     2581 ttgaagaatt ttttgtgaag tcaaaagaaa aaagaacttt ctttttttca gatgattcta
     2641 ctaccgcttt tcgtatattc aatggagaag gagacggaat cggcggtctg attgttgatt
     2701 attatgcagg ttatgctgtt ttttcttggt ataacgaaac gttatatgca catcgtgaat
     2761 tattgattca agcttttcgt gaaatctacc cggaaatcgt aggaggctat gagaaaattc
     2821 gatttgaatc agtaaatctg cctgagagcc agttcattta cggaaaagag gcaccggaac
     2881 ccttgatcgt aaaagaaaac ggagtaaaat ttgccgttta tttaaatgaa ggattaatga
     2941 ctgggatctt tttagaccaa aaagaagtac ggggatcatt agcagaaggt ttcgcttctg
     3001 ggaaaacagt tttgaacatg tttagttata ccggcgcctt ttctgttgct gccgcaatgg
     3061 gaggggcaat cgaaacaaca agtgtcgatc tagcaaaacg cagtttaaaa aaaacacagg
     3121 aacagtttga agtgaatggt ttggatgtcg atcagcaaaa aatcattgtt atggacgtat
     3181 tcgagtattt caaatacgcc aaaagaaaac aatttttata tgacatgatt gtattggatc
     3241 caccaagttt tgccagaaat aagaaaaaag tctttcgcgt agcaaaagat tatggggaac
     3301 tggtcaagga tagtctggat attttagcag aagacgggat acttattgcg tctacaaatg
     3361 ctgcgaatgt ctcgatagaa caattccaaa caatgattga agaggaattc catgccaaaa
     3421 atgtttcttt tgaacaactt gctttatatc gtctgccaaa agatttccag acgacggata
     3481 cctttttaga aggcaattat ctgaaagtgt ttgtttatca aattaaaaat aaccattaga
     3541 aggagcgatg cagaatggtt tgggtaaagg gattgaatat aggaagcggt agaccaaaga
     3601 tttgtgcgcc tcttatcggg gccgtccgag aggaagtatt acgtgaagca gttcttgcac
     3661 gtgaagcagg ggccgattta gtcgaatggc gagtcgatca ttatcgtgaa gttttttctt
     3721 ttgatgaagt agcagaaaca ttggttctta tccatgagat gcttgatgga ctgccgttat
     3781 tatttacgtt tcgtacctta gctgaaggcg gagaacaaga catatcgatc cgtcagtatc
     3841 gcgaccttta tgaacatgtg atacacacag gattagtaga tatggtcgat atcgagttgt
     3901 tcaaagtaga aagtctagga aaaagactga tagaggaaat caagtcttta aacattcctt
     3961 taattatcag tagtcatgat tttaaagaaa caccagcaga ccctgtcttg ctttaccgat
     4021 tgaatgttat ggaacatttt ggagccgata tcggaaaact ggcagtaact cccaataatg
     4081 aacgagatgt tttgcggttg atggagttga ctagacgggc aaatgctttt gtctccatgc
     4141 ctattattac gatgtccatg ggaaatcttg gtaaaatttc tcgtctggct ggcggaacga
     4201 cgggttccgt tatgacattt ggttccttaa gcagcgattc tacttctgct ccaggacaat
     4261 taccggtcaa agcattgaag caaattattc aattattaaa tgaaccgttg tagaacaagt
     4321 ttgaggtgat attttttggc aaagaaaaaa atagtaaaaa cgaatgcaat ccgtatggtc
     4381 gaacaaaaaa agattcccta tacagagcac gaatatgagt gggacgaaag ccacctaagt
     4441 gcgtcatcgg tcgctgaaca gcttccagaa agccaatctc gtatatttaa aacactggtt
     4501 gctgtgggca atgtgacggg gcctcttgtt gctgtgatac cgggagaagc cgaattgaat
     4561 ctaaaaaaat tagcaaaagt ttcagggaat aaaaaagtag aaatgctcca tttaaaagat
     4621 ttggaagcaa cgaccggata catacgtggt ggctgctcgc ctataggtat gaaaaaacta
     4681 tttcctactt atttagatca aattgcagaa agctatgaac aaatcattgt ctcggctggc
     4741 agaagagggc tacagatgga gttagcacct caagatatct gtgcattaac gagcggacaa
     4801 tttgcagata taaaacaata aatcatacgt ataaaagaaa gaaggaggcc gggatgttgt
     4861 ttgtccagtc tccttctttc ttttttatac attatgctct ttgcccggca ttcattcctg
     4921 ctacatgccc tgtagcgaaa gccgctgtga tattaaagcc gccagtataa ccattgacat
     4981 ccagtaattc tcccgcaaaa tacagaccat caatgatttt actctccaat gttttaggat
     5041 aaatttcctt tagagaaacc cctccgccag ttacaaatga tttctcaata ggaaaagttt
     5101 tgttgattga tagcttaaac tctttgcata gttgaacaaa atcttgaatt tgtttttgag
     5161 aggcttgttg tcctgtcaga tgatccattt caagccgctc aagaaagaaa actaacaatc
     5221 gctcaggcaa tataccatgc caagcattga ccaagttctt tttggttgct ttactttttt
     5281 ctgtcaatat atgaataagc tcttgtttgg tttgtgtagg gaaacaatcc agagaaagtg
     5341 tcactggttc tcccgttttc tctaattctt tattgacaaa acttgaacaa cgcagtgcag
     5401 ccggaccaga aataccgaaa tgggtgaaaa gtaaatccat cgtatgttca gtcaatacac
     5461 gacctttttt atttaaaacg cgtaaagtga tgtcttgtaa agaaagccct tgaagtgttt
     5521 tctcctgaat atacggttca tccgatatca agggggactc tgtcgcataa agaggcgtca
     5581 ccgtgtgacc cactcgcttc acgatcttgt acccatctcc agtagaacca gtagatggat
     5641 aagtccgtcc accagttgtt aagataatgg caggagcatg gaactcttcg aattccgtgc
     5701 gtataccgta aattttatgt tctttgtgga tcaatttctc tactcgagta gagaatagaa
     5761 gcgtcacatc tagttctttc agacgattga gcaatgcttc gataatcgtt tttgatttat
     5821 ttgttacagg aaacatccgt ccatgatcct cttcttttaa atgtacgcct tgtgattcaa
     5881 agaagttcat gatatcgaaa ttattccatt gcgcaaacgt actatataaa aactttccat
     5941 ttccaggaat atgtgcaatc aaatcatcaa caggacgatt gttcgtaaca ttgcaccgac
     6001 caccacctgt catcagtagt ttttttcctg cttttttgtt tttttcgatc aagagtacgc
     6061 gtgcgccttg ttcagctgca ctgatcgctg ccatcatccc gcttgttcca gcgccgacta
     6121 cgatcacatc atacagtttt tccattccct cacctcaagg aaagtgtagc atatctgaag
     6181 aaagaaaaca gaaaaaaaat gttttgagcc aaaagagaaa atgagctaaa atgagggaag
     6241 ataagcaaag gagcgtggaa cgatgacaaa cgcaacagaa tatgttcaag caattcaaga
     6301 aaagctacat caaaaggatc aagggcaaga agaattttta caagcaatcg atgaattcat
     6361 gcccacggtt atgtcttttt tggacactca tcccgaatat atcgaaaaga atatcttaga
     6421 acttttaacg gaaccagaaa gagttattca atttcgagtg ccttggcaag atgatgaagg
     6481 aaactggcga gtaaatagag ggtaccgtat ccaatacaac tcagctatcg gcccttataa
     6541 aggtggattg cgcttccatc ctagtgtcaa tctaagtatc ttgaaatttt tggcttttga
     6601 acagattttc aagaatagtt taaccggtct gccaatcggt ggaggaaaag gcggaagtga
     6661 cttcgatcct aaaggaaaat ctgataatga gatcatgcgt ttttgtcaaa gtttcatgct
     6721 ggaattgtca aaacatatcg gcccatcgat tgatgttcct gcgggagata tcggtgtagg
     6781 agctagagaa atcggctatt tgttcggcga atataaacga ttgaaaaaat atgatactgg
     6841 tgtgttgaca ggtaaacctt tggatttttg gggaagtaag atacgtacgg aagctactgg
     6901 ttatggatta gtgtattatg tcaaacatct attgaacgaa gaaaaagatt catttaatca
     6961 aaaaacagtt tttgtttccg gaagcgggaa tgtagccatt tatgcaatcg aaaaagtgca
     7021 agagctaggc gggaaagttg ttacatgttc agattctagc gggtacatct atgatccaga
     7081 aggaatagat gtgaaactat tgaaagaagt caaagaaatc aatagacaac gtttgacgga
     7141 atacgcaaac caacgtcctt ctgctattta tcatgctgga gaatctgttt ggacattgaa
     7201 agaaaaagcg gatatcgctt tgccatgtgc tactcaaaat gaaatagacg aaagcttggc
     7261 cgaaatctta gtagaaaatg ggatcaaaat cgtagcagaa ggcgctaata tgccatgtac
     7321 agtaaaagct gtttctgttc ttcatgaagc aggcgtgtgg tattgtccag gaaaagctgc
     7381 taatgctgga ggagtagcag tatctgcttt agaaatgagt cagaacgtcc agcgattgac
     7441 ttgggaaaga gagcaagtgg atgagcagct ggatcaaatc atggaaacga tttaccatac
     7501 gtgtcgccaa acagcagccg agtacggcga cgaaaaaaat ctgcttcttg gagcaaatgt
     7561 tgctggattt gaaaaagttg ccaaagcaat ggcagcagaa ggtttagttt gaaaaatgaa
     7621 actttcttgg aaatattgtc agcattttgt ttatttgctt gaaaaataat aaaaaaaaac
     7681 gtaaagtatt acctgtagag taaacctata aggaggagaa caaatggcac acattcattt
     7741 tgactattca aaagtagcac cgtttgtcaa cgaacaagag ttagggtaca tgcaaagcca
     7801 agtaacagca gcccacaacg aattacggga aggtactggc gcaggaagcg acttccgcgg
     7861 ctggatcgat ttaccaacga actatgacaa agaagaattt gcacgtatca aagaagcagc
     7921 taaaaaaatc caatctgatt ctgaagtatt agttgttatc ggtatcggtg gttcttattt
     7981 aggcgctcgt gcagcgatcg acttcttgaa tcacacattc ttcaatttat tagatagcaa
     8041 cacaagaaaa gcaccacaaa tcttctttgc cggaaattca atcagctcaa catatattgc
     8101 tgacttgatc gaagtaatcg gtgatcgcga cttttcagta aacgttattt caaaatctgg
     8161 aacaactact gaaccagcga ttgcgttccg tgtattcaaa gagcttcttg ttaagaaata
     8221 tggacaagaa gaagcaaaca aacgtatcta cgctacaaca gataaagcaa aaggtgcagt
     8281 taaagtagaa gcagatgcag aaggttggga aactttcgtg attcctgatg acatcggcgg
     8341 acgtttctct gtattaactg ctgttggttt gttaccaatc gctgttagtg gtgcagatat
     8401 cgatgcattg atgcaaggtg ccgctgatgc aagcaaagca tactcaagcg acaaactaga
     8461 agaaaacgaa gcttaccaat acgcagcaat gcgcaacatt ctttaccgta aaggcaaagt
     8521 aactgagttg ttgatcaact atgaaccagg aatgcaatac ttctcagaat ggtggaaaca
     8581 attgtatggt gaatctgaag gaaaagacca aaaaggcatc tatccttcaa gcgcaaactt
     8641 ctcaactgat ttgcactctt taggacaatt catccaagaa ggacgtcgca atatttttga
     8701 aacagttgtg aaggttgaaa aacctcgtaa atcaatcaca attcctgaac aagcagaaga
     8761 tttagacggt ctaggctact tgcaaggaaa agaagtggac ttcgtaaata caaaagcatt
     8821 tgaaggaaca ttgcttgctc atacagacgg tgacgtgcct aacttgctag tcaaaattcc
     8881 agaaatggat gcttatacat tgggttatac aatgtacttc tttgaaattg ctgtaggtat
     8941 ttcaggttac ttgaatggaa tcaacccatt cgaccaacca ggtgtagaag cttacaagaa
     9001 aaacatgttc gcacttctag gcaaaccagg ctttgaagat cttgctaaag aattgaacga
     9061 acgcctgtaa gataagggtt tttcgctaga tttaaataat cagtgataga aacatgttcg
     9121 ctttaaaatg aaaactggta cccatttagg tactcaaaca tactgcatcg tttattcaga
     9181 ataaggggct gtgacaagac gtttgtcaca gtcccttgtt tgtatagaaa tcgattgatt
     9241 gccttgctct ttagtttgtt ttaggacttt aagaacagtg aggctgactt cgaaggttaa
     9301 ttctgttttt ttgttttcta gaatcagtaa gcaaaaaagc actcacttcg agtgcttttt
     9361 tgcgatatat tcttagctta agataggcct aatttttctt ttacagatga gggtacctta
     9421 tcgctggtaa ttactttttt ctctatgatc cgcttttcgc ttgctttcaa ctgcaagatc
     9481 gttccttcgg ggtaagcaga accattggca gagtcaccag aagtaatgac atcgaaatct
     9541 aactgtcttt tttcaccgtt ttcagcataa gcatctacgt tgtatttaaa acctcttcca
     9601 attacttccc cattatctgc tttgatatca gtttctgcag gcattggagc tttgactacc
     9661 gcataataat ctttacctac ataagtagac tgatagtagt cccaaccttt ccatcctata
     9721 aaagcaatca atatgataac gatcaaccct aaaatttttt tcatgaaaga tctccttttt
     9781 tatttttatt atacccgtgg tgctagttaa ttttaatatc ctaaagaata tctcaaagtt
     9841 ccttcaaaac gtcgttgtgc tcgacttaat agaacactgt aagccagtaa tatgctttca
     9901 aatctactct ctagcccttt aacagaacgt gagttgaaat tttgacaacc taatttttct
     9961 aaggaagaaa aaacagtctc aactgt
//
LOCUS       DACDXO010000081         9623 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E23605_2017
            SAMN12639989-rid9881463.denovo.082, whole genome shotgun sequence.
ACCESSION   DACDXO010000081 DACDXO010000000
VERSION     DACDXO010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639989
            Sequence Read Archive: SRR10023389
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9623)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741914.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:42:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,972
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 33 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 17 of 73
            Pseudo Genes (multiple problems)  :: 10 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9623
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639989-rid9881463.denovo.082"
                     /isolate="E23605_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            125..1099
                     /locus_tag="IWM35_002413"
     CDS             125..1099
                     /locus_tag="IWM35_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP9920238.1"
                     /translation="MRQLHKEQIETAMAALKEKTIDHVYFTACGGSQAVLMAGQYLFD
                     KELTVPCHVYTANEFNYAIPKGLSENSLVITCSHSGTTPETVEATKKAREFGAMTICL
                     SNEEGSPLWEAAEYPVHYDWGKDVDASDLNKGILYGLLFNILLVLDGSKKWETCLNEF
                     DKLNDLVIAEKSKFEITAKEWGKNQKRDKIIYTIGSGINYGEIYSTAACWFMEMQWIN
                     SNAIHSGEFFHGPFEITDYDVPFILVKSIGDTRFLDQRVEDFTKKFSNKLMVLDQADF
                     DLESIDSVAKEYIAAVLSGVVIRQMVEAIAFERGHSLDVRRYMWQMKY"
     gene            1081..1542
                     /locus_tag="IWM35_002414"
     CDS             1081..1542
                     /locus_tag="IWM35_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAP9920239.1"
                     /translation="MANEILINRRLQKMANQLFHIVITTHSTLCEGYLKATKLILMND
                     ATGISAVPFKENTSPDDFEESMKEIISQHHLQPLIILTDLIGGTPNNVSIKQLNNNRI
                     QIIAGINLPLLLELLMAQSAGKSIEEIDFKELLNNSQKSMVHINSLLKDVS"
     gene            1592..2056
                     /locus_tag="IWM35_002415"
     CDS             1592..2056
                     /locus_tag="IWM35_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP9920240.1"
                     /translation="MIKLVRVDHRLLHGQVIFSWTKQYDINHIIVADNKVPGDPMSVM
                     ALTIAKPADCKLDIVKISDVKGIMETSPNDNYMILIKGPKEAFELVQNLPEIKEINFG
                     GVSKKSESKQYGKAVFLTPEELEATKKILDQEVKIFVQQVPSSSIENVDFSK"
     gene            2070..2819
                     /locus_tag="IWM35_002416"
     CDS             2070..2819
                     /locus_tag="IWM35_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP9920241.1"
                     /translation="MLLKAILLGLVGVVAVIDSRLIGRQNIGRPLILSTLAGLVLGDL
                     HTGIVLGASLELISMGFVSIGAAGPPNMQLGSIIATAFAILTNSSTETALTIAIPVAV
                     AGEFMSIIMRMIIAQFGHAADKAIENNKFRKAQIIHIYWSFIFNAFVYFIPIFLTVYF
                     GADVVADLVDKIPEMITDALTVSGNMLSALGFAMLLSTMLSKKLYPYFIFGFFIVAYS
                     GLSLIGVTIFAALIAFVMDQVKYGKREEAHG"
     gene            2819..3649
                     /locus_tag="IWM35_002417"
     CDS             2819..3649
                     /locus_tag="IWM35_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP9920242.1"
                     /translation="MENSTEKKRLITKKDLNKMCLRSWTIMCSWNYERQMHMGFMYGM
                     APILNKVYKNDEEKKREAYTRHMAFFNCTPQMTSFIMGLCASMEEQNAQAEEGNFNTE
                     SISMIKTSLMGPFAGIGDSFFQGTLRIITFGIGLSFAKQGSILGPILAVGLFAIPSIL
                     AVFYGGKLGYESGNKYLTKLYQEGLMDRVMQFASIIGLAVVGGMVASMVPIVTPLKFT
                     TGGTELVLQDLLDSIIPGLLPLVCTLGIYKLIKKNVSTNMLLLGCILAGFVLSIIGIL
                     "
     gene            3649..4296
                     /locus_tag="IWM35_002418"
     CDS             3649..4296
                     /locus_tag="IWM35_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP9920243.1"
                     /translation="MIKALIFDMDGVLIDSEIMYQKWVFNFLKQENLSYPLYSYQKKI
                     GTTASIFDDIEKYNEGCNSLELKGKFTKYWKSQKIDYLDIFPNILIEQLEWLKKKKIK
                     MAIASSSPMDTINEVVTTCKIESYFDCLISGRDLPESKPNPTIFLKASEQLRIPVEEC
                     LVIEDSYNGIKAGKRANMKVLAIKDKRFSQDVSLADDVIYDIKYLRQEVQVKFGI"
     gene            complement(4394..5170)
                     /locus_tag="IWM35_002419"
     CDS             complement(4394..5170)
                     /locus_tag="IWM35_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP9920244.1"
                     /translation="MNFQDNRPLYLQVEASIRNDIREKKYLPGEQLPTEEELCKIYGV
                     SKITIRKAFKLLAESGTVERLRGKGTFVTQKKESLKLAGVHGFSDSLSTRGHKIRYTV
                     LHSKIIKADEFLANKLQINVTDRVYNIKRLMWEDGAPMGIDNFFASEEKFPTLFEKGS
                     QYTSLYKLLEDDYDVKATSSIMEINGLSATPELSDLLQCIVGDPLFILEKVGKDKNNQ
                     PFHYSQSTVRCDRISYVIEVTENITLDTVHKNDSDPKVID"
     gene            5314..6273
                     /locus_tag="IWM35_002420"
     CDS             5314..6273
                     /locus_tag="IWM35_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP9920245.1"
                     /translation="MTYTHLTTDELVIIESYFNIEKPVALVAQSLNRSRQTIYNVYSF
                     LKQGKKVLDYYRQYKQNKSRCGRRSTVLSDEQSTYVQEKVAQGWSPDVIIGRGEFPID
                     CSVRTLYRMFKKGLFDTQSLPMKGKRKPNNHQEKRGKQAFRRTIHERNSLYPHLTKEF
                     GHLEVDTIVGVKHKSAVITLVECLSKVIITLKPTGRRAEDIENRLNDWFKSMPKHLFK
                     SMTFDCGKEFSNWRSISNSNDIAIFFADPGTPSQRGLNEHSNGLLRRDGLPKTMDFRD
                     TDETFIQSVASKRNHIPRKSLNYRTPLEVFLSCIDKDILSSLI"
     gene            6390..6698
                     /locus_tag="IWM35_002421"
     CDS             6390..6698
                     /locus_tag="IWM35_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HAP9920246.1"
                     /translation="MFQNKGQRYVTKGVMDSLPVELQALCWNLIDQNVQKQFHLDYLQ
                     IFEFSTEKGNQKLVHRQEEPEARKEYLISPKLRLKSVSQKIWVIDSGEYQTMLLPEEY
                     "
     gene            6725..7024
                     /locus_tag="IWM35_002422"
     CDS             6725..7024
                     /locus_tag="IWM35_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase"
                     /protein_id="HAP9920247.1"
                     /translation="MLETAIEVLEKCAQLVTTPEEWGYESVTMEKEEIEMGMLPEDVT
                     LPRLVMTHLYIYCAPEDGKDYVVYFITDITSQREFVRGLLVEGRLVWSQIGGTNE"
     gene            7198..7749
                     /locus_tag="IWM35_002423"
     CDS             7198..7749
                     /locus_tag="IWM35_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1643 domain-containing protein"
                     /protein_id="HAP9920248.1"
                     /translation="MIEKELVTTQTEVWRDPHHTHRYVFKRQWAIKGKEAEDKLAVVI
                     TIRPTDTEPFTNDLSMLLIEKNIRQLGFTGFIAVNLFSYTKAKSPRVFPRGNDEQSLE
                     ILTTVFTEKKINTIIFACGSITATSQVAYEQAKTIYDQLSAKQKKMTKVLINAEGKLS
                     HPLSIHVRNEWQLADSQLLFQEK"
     gene            7895..8188
                     /locus_tag="IWM35_002424"
     CDS             7895..8188
                     /locus_tag="IWM35_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9920249.1"
                     /translation="MEIDETCLSNSYKSMNDFFEEMELDSELLYQFYLAFRGQQISFP
                     MKMYDRELVRKRIENMIEQEKTVDIRQLTEVYGFSARWIQKVIKQKERRRVHG"
     gene            8181..8477
                     /locus_tag="IWM35_002425"
     CDS             8181..8477
                     /locus_tag="IWM35_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9920250.1"
                     /translation="MAKLIVLGGLIYGGYWLYKYMLAHPNIDKGKVIKTVGVIIVGLI
                     ALTKKNNTNSPKIMRTYRCRGCGREISTSFNPSESPQGRDRCHAGYRHSWERIN"
     gene            8548..9552
                     /locus_tag="IWM35_002426"
     CDS             8548..9552
                     /locus_tag="IWM35_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HAP9920251.1"
                     /translation="MKKWLLLLIGMGILFTGCTSENTRTKETVETDTTIEAKKSKTQS
                     TEQTTTEMKQSVEASTSESSIIESSSEEVKNTLWDSEKSTQLAIFVPQWGKTLGQEYK
                     SYNPQNNVSLYGTPLPSAVIDGNWKMAINEAPVTVQWSEDGTGHADFNLVAVYSDVET
                     GEYLGQHVYFFGFQNGQPKVYLTQQNQGNDKNYMYFNETQNQQLKQGFIDIVNGQTPQ
                     TPVVEESTQQATTTTTQSTSSSAISSWEAAKETVLNNKALWLYDSPDKNASNTNLIST
                     NEYMKDLMQDENGSYYSINIVTDDNGNTTMAGGKQFRVYTDGRISQRIGMTNNFQMIK
                     "
BASE COUNT         3410 a         1373 c         1797 g         3043 t
ORIGIN      
        1 atataaaatt gttataaaaa aatttttttg aaaaatagta ttgcaaaata aagcaatttc
       61 atagataata ggtattgtaa agttatatga ctttataata cttattagag tgagggataa
      121 agaaatgaga caactacaca aagaacagat tgaaacagca atggcagcgt taaaggaaaa
      181 gacaattgat catgtatatt ttacagcatg tgggggttca caagccgttt taatggctgg
      241 tcaatatcta tttgataaag aattaactgt accttgtcat gtttatactg ctaacgaatt
      301 taactatgct ataccaaaag gattatctga aaattcttta gttatcactt gttcccattc
      361 tggcacaaca ccagaaactg tagaagcaac aaaaaaagca agagaatttg gagcaatgac
      421 tatttgttta tctaatgaag aaggatctcc tctatgggaa gcagctgaat atcctgttca
      481 ctatgattgg ggtaaagatg ttgatgctag tgatttaaat aaaggaattc tttatggttt
      541 attatttaat attttacttg ttttagatgg ctctaaaaaa tgggaaactt gcttgaatga
      601 atttgataag ttaaatgatc ttgttattgc tgaaaagagc aaatttgaaa taactgctaa
      661 agaatggggt aaaaaccaaa aacgagataa aattatttac acaattggta gcggtatcaa
      721 ttacggagaa atttactcca cagcagcttg ctggttcatg gaaatgcaat ggattaattc
      781 taatgctatt cattctgggg aatttttcca tggtcctttt gaaatcactg attatgatgt
      841 accattcatc ttagtgaaaa gtattggaga tacaagattt ttagatcaac gggtagaaga
      901 cttcactaaa aaattcagta ataaattaat ggttttagac caagctgatt ttgacttaga
      961 atctatagat agtgtagcga aagaatatat tgcagctgta cttagtggcg ttgttattcg
     1021 ccaaatggta gaagcgattg cttttgaaag aggccattcc ttagatgtta gacgctatat
     1081 gtggcaaatg aaatattaat taataggagg ttgcaaaaga tggcaaatca attatttcac
     1141 attgtgatta caacacattc taccctttgt gagggttatt taaaggcaac aaaattaatt
     1201 ttaatgaatg acgccacagg tattagtgct gttccattta aagaaaatac tagtccagat
     1261 gattttgagg agagtatgaa agaaataatt agtcagcatc atttgcaacc tctaattatt
     1321 ttaacggatt taattggagg aacacctaat aatgtttcta ttaaacagtt gaacaacaat
     1381 agaattcaaa ttattgctgg gattaattta cctcttttac tggagttatt aatggcccag
     1441 tcagctggta aatctataga agaaatagat tttaaggaat tactgaataa ctctcaaaaa
     1501 agtatggttc atattaattc gcttttaaaa gatgtcagtt aaggtggcaa tttctattct
     1561 ataaatttta aaaattgaag gaggaaatag aatgattaaa ttagtgcgtg tagaccacag
     1621 attacttcat ggacaagtaa tattttcttg gactaaacag tatgatatta accatattat
     1681 tgtggctgat aacaaagttc caggagatcc aatgtctgtg atggctttga caattgctaa
     1741 acctgctgat tgcaaattag acattgttaa aattagtgat gttaaaggga ttatggaaac
     1801 atccccaaat gataattaca tgattttaat taaaggacct aaggaagctt ttgaattagt
     1861 tcaaaattta ccagaaatta aagaaattaa ttttggcggt gtgtctaaga aaagcgagtc
     1921 caaacaatat ggaaaggcag tctttttaac tcctgaagaa ttagaagcca caaaaaaaat
     1981 tcttgatcaa gaagtgaaaa tatttgtgca acaagtccca tcttcaagta ttgaaaatgt
     2041 agattttagt aagtaagggg ggaaaataaa tgttattaaa agccatttta ttaggtttag
     2101 ttggtgtggt agctgttatt gactccaggt taattggtcg acaaaatatt ggtagaccac
     2161 taattttgag tacacttgcc ggacttgtgc taggtgactt gcatacaggg attgttcttg
     2221 gggcatcttt agaattaatt tctatgggat tcgtctcaat tggggcggct ggacctccta
     2281 atatgcaact tggtagtatt attgccactg cttttgcaat tttaactaat agtagtacag
     2341 aaacagcctt aacaatagct atcccagtgg ctgtagctgg tgaatttatg agtattatca
     2401 tgcgcatgat aattgctcaa tttggacatg ctgcagataa agccattgaa aataataaat
     2461 ttagaaaagc tcaaattatt catatttatt ggtcatttat ttttaatgct tttgtttact
     2521 ttattccaat ctttttaaca gtatattttg gtgctgacgt tgtggcagat ttagtagata
     2581 aaattccaga aatgattaca gacgccttga ctgtctctgg aaatatgctt tccgcattag
     2641 ggtttgcaat gctattaagc acaatgttga gtaaaaaatt atatccttac tttatttttg
     2701 gattctttat tgttgcctat tcaggtcttt cactaattgg cgtgacaatt tttgcagctc
     2761 tgattgcctt tgtaatggat caagttaaat atggaaaaag agaagaggcg catggttaat
     2821 ggaaaatagt acagaaaaaa aacgtttaat cacaaaaaaa gatcttaata agatgtgtct
     2881 tcgttcatgg acaatcatgt gttcgtggaa ttatgaaaga caaatgcata tgggatttat
     2941 gtatgggatg gcgccaattt taaataaagt ttataaaaat gatgaagaga aaaaaagaga
     3001 agcttatact cgtcatatgg cattttttaa ctgtacgcct caaatgactt catttattat
     3061 gggactttgt gcttctatgg aagaacaaaa tgctcaagca gaagaaggaa actttaatac
     3121 tgaatctatc tccatgatta aaaccagttt aatgggtcct tttgctggaa ttggtgattc
     3181 atttttccaa ggtactttaa gaattattac atttgggatt ggattgtctt ttgctaagca
     3241 aggtagtatt ttaggaccga ttttagctgt tggccttttt gctatacctt ctattttagc
     3301 tgttttttac ggcggtaaat taggatatga gtctggcaat aaatacctta ctaaattgta
     3361 tcaagaaggt cttatggatc gagtaatgca atttgcttct atcattggtt tggctgtagt
     3421 aggtggaatg gtggctagca tggtgccaat tgtcacccct ttaaaattca ctacaggggg
     3481 aacagaactt gttttacaag acttacttga ttccattatt cctggattat taccacttgt
     3541 atgtactttg ggaatatata aattgattaa gaaaaatgtt agtacaaata tgctcttatt
     3601 aggttgtatt ttggcagggt ttgttttaag tataataggt atcttataat gattaaggct
     3661 ctaatttttg atatggacgg agttttaatt gatagcgaga ttatgtatca aaagtgggta
     3721 tttaattttt taaaacaaga aaatttaagc tatcctttgt attcttatca aaaaaagatt
     3781 ggaactacag caagtatttt tgatgatata gaaaagtaca atgagggatg taacagttta
     3841 gaattgaagg gaaaatttac taaatattgg aaatcgcaaa agattgatta cttggatatt
     3901 tttcctaata tactaattga acagcttgaa tggttaaaaa agaaaaaaat aaaaatggca
     3961 attgcttctt ccagtccaat ggatactata aatgaagttg ttaccacttg taaaatagag
     4021 tcatattttg attgtttaat tagcggaaga gatttgccag aaagtaaacc taatcctact
     4081 atattcctta aagcttcaga acagctcaga attccagttg aagaatgtct ggtgatagaa
     4141 gattcttata atggtataaa agctggtaaa agagcaaata tgaaggttct tgcaattaaa
     4201 gataaacgtt tttctcaaga tgtaagtcta gcagatgatg ttatttacga tattaaatat
     4261 ctgcgacaag aagtacaggt gaaatttgga atataaataa gttttttgat gtatttagag
     4321 aatattagta aaaataaaaa tcatgcccaa atctaatcaa aattgattgc tttgggtatg
     4381 atttctttta atatcaatct attactttag gatctgaatc atttttatgc accgtatcta
     4441 aagtgatatt ttctgtgact tcaataacat aactaattct atcacaacga actgtggatt
     4501 gagaataatg aaatggttga ttatttttat ctttgccaac cttttctaaa ataaatagcg
     4561 gatctcctac tatacattgc aataagtcgg aaagctcagg tgttgcagat aaaccgttta
     4621 tttccataat agagctcgta gctttgacat catagtcatc ttctagaagt ttatataatg
     4681 aagtatattg gctacctttt tcaaataaag ttggaaattt ttcttctgat gcgaaaaaat
     4741 tatcaattcc catcggggca ccgtcttccc acattaaacg tttaatattg taaactctat
     4801 cagttacatt gatttgtaat ttattagcta aaaactcatc agcctttata attttagaat
     4861 gtaaaacagt atatctaatt ttatgtcctc gggtagaaag agaatcgcta aatccatgaa
     4921 ctccagctaa ttttaaactt tctttttttt gagttacaaa ggtccctttc ccccttaatc
     4981 gttcaactgt tccactttct gctaaaagtt taaaagcttt tcgaatggta atcttactta
     5041 ctccataaat tttacataat tcttcttctg taggtaattg ctctcctgga agatattttt
     5101 tttcacgaat atcgttacgt atagaagctt ctacttgtaa atataatggt ctattatctt
     5161 gaaaattcat agtacactcc ctaaaaagtt atacttcatt atatcacaat ttattaaaac
     5221 gtcgattgta aaattaagct agacaactaa aaagatcatt tgtgctacac tcaaaatagt
     5281 tccgaccaaa gaattaataa ggagtgagta caaatgactt acacccatct tacaacagac
     5341 gaactagtaa tcatagagtc ttatttcaat atcgagaaac ctgtcgctct tgtcgcacaa
     5401 agtttaaatc gctctagaca aaccatttat aatgtttatt cgtttctcaa acaaggaaaa
     5461 aaagtgctag attattaccg tcaatacaaa caaaataaat ctcgttgtgg acgacgttca
     5521 accgttttat ctgatgaaca atcaacatat gttcaagaaa aggtagctca aggatggtca
     5581 cccgatgtga ttatcggccg tggcgagttt ccaattgatt gttctgttcg tacactctat
     5641 cggatgttta aaaaaggcct ttttgacaca cagtcgttac ctatgaaggg gaaacgtaaa
     5701 ccgaataatc atcaagaaaa gcgtggaaaa caggcctttc gcagaacgat tcatgaacgg
     5761 aacagtctct atccacacct gactaaagaa tttggtcatc tcgaagtcga caccattgtg
     5821 ggagttaaac acaagagcgc tgttattaca ttggttgagt gtttatccaa ggttattatt
     5881 actttgaaac cgacaggcag acgagccgaa gatatcgaaa acaggttaaa tgattggttc
     5941 aaatccatgc ctaaacactt gtttaaatcc atgacatttg attgtggaaa agaattttcc
     6001 aactggcgat ctataagtaa ctcgaacgat attgccatct ttttcgcaga tccaggaaca
     6061 ccttcgcaaa gagggctaaa tgagcactca aacggcttgc ttagacgaga tggcttacct
     6121 aaaacaatgg actttaggga cacggatgaa acctttattc aatccgttgc ctcgaaaaga
     6181 aaccatattc caagaaaatc attaaattat cgaacaccat tggaagtatt tttgagctgc
     6241 atagataaag acatattgtc tagcttaatt tgacaaatca gatagtataa aatgaatcaa
     6301 attgaatatc aaatatatat tatatccatg aagcccaatc ttctttaata gaagattggg
     6361 ctatttttat ggcaaaggag ggaaaagaaa tgtttcaaaa taaaggacaa cgttatgtca
     6421 caaagggagt aatggacagt ttgccggtgg aattacaagc tttatgttgg aatctaatcg
     6481 atcaaaacgt tcaaaagcaa tttcatttag actatcttca aatctttgaa ttttctacgg
     6541 aaaaaggaaa tcaaaagttg gttcatcgtc aagaagaacc agaagcaaga aaagagtatc
     6601 tgatttcacc taagcttcgt ttgaaatcag tgtctcaaaa aatatgggtc attgattcgg
     6661 gtgagtatca aacgatgtta ttaccagaag aatactgata tctaaaagaa aggaacgaaa
     6721 ggacttgtta gaaacggcta tcgaagtact ggaaaagtgt gcacagctag tcactactcc
     6781 tgaagagtgg ggctatgaat ccgttacgat ggaaaaagag gaaattgaaa tgggtatgct
     6841 tcctgaagat gttactctac cacgtttagt gatgacacat ctttatattt attgcgcacc
     6901 agaagatgga aaagactatg tagtttattt cattacagat attaccagcc agcgagaatt
     6961 tgttcgtggg ttactggtag aaggacgatt ggtttggagt cagattggag gaacgaatga
     7021 ataagatcga ttatttgaga atgattgcga aatgtttaga agatatcctt acagcgattc
     7081 atcaaattct gaagaaaaaa ggagaaagaa tatgaaatat tttcaactag tattgacact
     7141 tataacggca atggtgattg aattgcttga acaaaaatta gcataggaga aaaagagatg
     7201 attgaaaaag aattagtgac cacacaaaca gaagtttggc gagatcctca tcatacccat
     7261 cgctatgttt tcaaacgaca atgggcgatc aaaggaaaag aagcggaaga taaattagcg
     7321 gtagtcatta ctattcgtcc aacagataca gaaccattta cgaatgattt gagtatgtta
     7381 ttaatcgaaa agaatatccg acaattagga tttactggtt ttattgctgt gaatcttttt
     7441 tcgtatacaa aagcgaaaag tcctcgggta tttcctcgtg gaaatgatga acaatctttg
     7501 gaaatattaa caactgtttt cacagaaaag aaaatcaaca ccattatttt tgcttgtggt
     7561 tcgattacag cgacgagtca agtggcttat gaacaagcga agaccattta cgatcaattg
     7621 agtgcgaaac aaaaaaagat gaccaaagta ttgataaatg cagaaggtaa gttatcccac
     7681 ccactaagta ttcatgttcg taatgaatgg caattggcag atagtcaatt attatttcaa
     7741 gaaaaataac attttatttt cttatgttac ttataagtag ttttacatgc tttttgactt
     7801 taaaaataca tgtgatgata catgttatat gaattatcaa tgtacccagt aatatgctat
     7861 tttcattatg taaaaaataa cagggggtgt gtagatggag attgatgaaa cttgtttgag
     7921 taactcttat aaaagtatga atgacttttt tgaggaaatg gaactggatt cagaattgct
     7981 ctatcaattt tatcttgctt ttcgagggca acagatttct tttccaatga aaatgtacga
     8041 tcgtgagtta gttagaaaaa gaatcgaaaa catgatagaa caggaaaaaa ctgtagatat
     8101 ccgtcaattg acagaagttt atggtttctc tgcaagatgg attcaaaaag taattaaaca
     8161 aaaagaaagg agacgagtac atggctaagc tgattgtatt aggtggttta atttacggtg
     8221 gatattggtt atataaatat atgcttgcgc atccgaatat agataaagga aaagtaatta
     8281 agacagttgg ggtaatcata gttggactca tagctctaac taagaaaaat aatactaata
     8341 gcccaaaaat aatgagaact tatcgctgta gaggatgtgg aagggaaatt tctactagtt
     8401 ttaatccatc tgaaagccct caaggaagag atcgttgtca tgctggctat cgacattctt
     8461 gggaaaggat taactaaatg aacaccattg agattaattg actgataaaa taagtacgcg
     8521 agggagaaat tgaaaggggg aaataaaatg aaaaaatggt tgttattact aattggaatg
     8581 gggattttat tcactggttg tacatctgag aatacgagga ctaaagagac tgtagaaact
     8641 gatacaacaa ttgaggctaa aaaaagtaaa acgcaatcaa ctgaacaaac tacaacagaa
     8701 atgaaacaat ctgtggaagc aagtacaagt gaaagttcaa tcattgaatc ttcttctgaa
     8761 gaggttaaaa atactttgtg ggatagtgag aaatctactc aactagcaat ttttgtccca
     8821 caatggggaa agacattagg tcaagaatat aaaagctaca atcctcagaa caatgtaagt
     8881 ctgtatggta cgcctttacc aagtgcagtt attgatggaa attggaaaat ggcaatcaat
     8941 gaagcaccag ttaccgtaca atggtcagaa gatggaactg gtcatgcaga tttcaattta
     9001 gtagcagttt attctgatgt agaaactgga gaatatttag ggcagcatgt atatttcttt
     9061 ggctttcaaa atggacaacc taaagtttat ttaacgcaac aaaatcaagg caatgataaa
     9121 aactacatgt acttcaatga aactcagaac caacagttaa aacaaggttt cattgatatt
     9181 gtgaatggac aaactcctca aactccagta gtagaagaat ctactcaaca agcaacaact
     9241 acaacgacac aatctacaag tagctcagca atttcaagtt gggaagctgc taaagaaact
     9301 gtactaaata ataaagcttt atggttgtat gattctcctg ataaaaatgc atcaaatact
     9361 aatttaatta gcacaaatga atatatgaaa gatctaatgc aagatgaaaa tggaagctac
     9421 tattcaatta atattgtaac ggatgataat ggaaatacaa caatggctgg tggaaaacaa
     9481 ttccgtgttt atacagatgg aagaatttct caacgtattg gaatgactaa caattttcaa
     9541 atgattaaat aattgaaaat attatttatc aaagaaaaac tgattttgag aaaaattatt
     9601 taattttctt agaatcagtt ttt
//
LOCUS       DACDXO010000082         9360 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E23605_2017
            SAMN12639989-rid9881463.denovo.083, whole genome shotgun sequence.
ACCESSION   DACDXO010000082 DACDXO010000000
VERSION     DACDXO010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639989
            Sequence Read Archive: SRR10023389
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741914.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:42:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,972
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 33 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 17 of 73
            Pseudo Genes (multiple problems)  :: 10 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9360
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639989-rid9881463.denovo.083"
                     /isolate="E23605_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(91..759)
                     /locus_tag="IWM35_002427"
     CDS             complement(91..759)
                     /locus_tag="IWM35_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP9920252.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QLEH"
     gene            893..1180
                     /locus_tag="IWM35_002428"
     CDS             893..1180
                     /locus_tag="IWM35_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9920253.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            1596..3134
                     /locus_tag="IWM35_002429"
     CDS             1596..3134
                     /locus_tag="IWM35_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HAP9920254.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            3131..4096
                     /locus_tag="IWM35_002430"
     CDS             3131..4096
                     /locus_tag="IWM35_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9920255.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            4083..5405
                     /locus_tag="IWM35_002431"
     CDS             4083..5405
                     /locus_tag="IWM35_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP9920256.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     regulatory      5468..5572
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            5646..6389
                     /locus_tag="IWM35_002432"
     CDS             5646..6389
                     /locus_tag="IWM35_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9920257.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     gene            6404..7396
                     /locus_tag="IWM35_002433"
     CDS             6404..7396
                     /locus_tag="IWM35_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP9920258.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            7408..8145
                     /locus_tag="IWM35_002434"
     CDS             7408..8145
                     /locus_tag="IWM35_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9920259.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            8135..8857
                     /locus_tag="IWM35_002435"
     CDS             8135..8857
                     /locus_tag="IWM35_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HAP9920260.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
     gene            complement(8941..>9360)
                     /locus_tag="IWM35_002436"
     CDS             complement(8941..>9360)
                     /locus_tag="IWM35_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HAP9920261.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEQPVVQETVQTEK
                     QAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQLDSSYL
                     NGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT         3068 a         1607 c         1972 g         2713 t
ORIGIN      
        1 ttttttataa agaagatttt actgaataat atcattttag tttacataat gatatttctg
       61 taaattaaat aaagtagtga aaaataagaa tcagtgctct aactgttcaa gaggatcaag
      121 tggttcaaat gtttctgtag tctgttttgc ttccaattgg ggcaaaacag atccaatttc
      181 cagatacttc actttattca atgtataaga tagaatagaa cagctaagta atgtatacag
      241 aaaatgtgtg agatccaaat aaagcaagga gacaactaaa ataccaaaag taatcaagaa
      301 aagacttttg ccaatcttaa tatgaaaata tttttcaatg gcttgataaa tgatatctgt
      361 tccgcctgtg cttccgccga atcgaaagac gagaccgcaa ccaatgccag aaagaatacc
      421 tgtcatcaaa ctattgatca gtagactatc tacagcaaaa tgtatatgaa ggctctccca
      481 taaatgtaaa aagaaaacta gcgcaaatgt tccgacgatc gtccggagaa aagcgtgttt
      541 tccaaaaaga aacaatgaga taagcaataa aggtaaatta aaaagcagtg aagtgacaga
      601 aggagcgaat ccaaaaagat tatgcaaaag caaagcaata ccagtaaccc caccatctgc
      661 cagcttagat ggaatattga ttcctgtcac agctaatccg tagattcctg cgcctaaaat
      721 gattccgcac aattctgtaa ataaatttaa ttttttcatt tctagcctct tttcttttga
      781 aaattttcag caacttcttt attatactga cttatactct tacttcaaga ggaaaatgaa
      841 aaaaaattat ttttggctgt ttctttttat ttatcaaata aggagtggaa aaatgcgagc
      901 agcagactta ttgacaattc ttacaaaatc acccaaaaaa acagagtatc aaggagtttt
      961 tttggctcaa gaacaaaaac tggtgcctgt gacaatggtc cagataaata aagcaaatca
     1021 atttatcttt ttatttgaac acggcaaagc accacttgct atgaaagaca tctttctttt
     1081 tttgatgaag aaccgtgaga aagctgttta ttatcagtca aaagaagaac tcattcctct
     1141 ttatggagtg aaagagatag aaaataaagt ggtgatataa gtaaaaataa gaaaacaaga
     1201 cgtttatgat gtcaaaacaa ccatttttta catttttggc aaaaaagtgt catgatacag
     1261 tataatattc gttgaacagg ccaataaagg tgttctcccc cacgagattt ctactttatt
     1321 ggctgtttct ttttattaaa ataaaagttg tgtgtgtaaa aaagatatag ctgattccaa
     1381 gtgacaattt cgtatagatt tttatggtat tcgtagtgaa aaaacgagaa ccaaaaagtt
     1441 gatccgactt tgttgcttgg attctttttt tgtcagcata tttagaggta tttttttctt
     1501 ttttttgttc gtgctgtaag gttaaggcaa gacaagttag ttgggaaaaa ggaaagaaat
     1561 gtttatactt aaagaataac agaaaggaag gtcgcatgaa taattttata caaacattaa
     1621 gtgaaagaaa aggcgagttg ctggaagctg catttcaaca tctgtccatt tctctcattt
     1681 ctttgctgat tgcagcactg attgctattc ctttagcgat ttgggcagcc aatcataaaa
     1741 aaatggcaga agtcttattg cagatcacgg gtgtgctcca aacgattcct tcacttgcct
     1801 tattaggttt acttatccca tttgtaggga tcggaactgt tccagcattg attgcacttg
     1861 ttatttacgc tttgcttccg attttccaaa acacgtatat tggtctagct gaaattgacc
     1921 cgtctattga agaagcagcg gtagcatttg gtatgtccag aatgagacgc ttgctgaaag
     1981 tagagcttcc catcgcatta cctgtcatta tctcaggtat ccgaactgcc ttagttttaa
     2041 tcatcggtac ggctactttg gcagcattga tcggagcagg tggattaggg acatttattt
     2101 tgttaggtat tgaccgaaat acacctgttt tgacattgat tggtgcgatc agttctgcct
     2161 tgcttgcaat tatattcagc ggactgattc gctggttgca gcataagaaa cctcgatatt
     2221 ccatcattac tttagcagca gtggtgtgtt tatttggcgg cgtttcttta tatcagtctg
     2281 ccttgtttaa aaatgaaacc atcacgattg ctggaaaatt aggagcggaa cctgaaatcc
     2341 tgatccaaat gtataaagaa ttgattgaag acaacacaaa aacagaagtt gaattaaaac
     2401 cgaattttgg caagacgagt tttttgttca atgcactgga tcaaggtcaa atcgatatct
     2461 atcctgagtt cacaggtact gtcctagaaa gcttggtgaa ggtacctgaa gcaatgaaaa
     2521 gtgctgattt gacaaaagaa aaaacatatg acgaagcaga agaattattg aataagcaat
     2581 tcgatatgac attattaaaa ccaatggctt accaaaatac gtatgccttg gcagtcaaga
     2641 gatcttttgc gaacgaacat caacttaaaa agatttccga tttggcagct atctctgatc
     2701 agatcaaagc gggattcaca ctggaattta ttgatcgttc agatggatat aaaggaatcc
     2761 aagaagcata tggcttgaat ttttcaagtg tacaaagcat ggagccaagt ttgcgttacc
     2821 aagcaatcaa taatggagat gtcaatattg tggatgccta ttccacagat agccagctca
     2881 aggagtacga tctagtgact ctagaagatg atcgagggtt attcccagct tatcaaggcg
     2941 caccgctgat gaaaaaagat tttgctaaag aacatcctga aatcgttaaa gcactgaata
     3001 aattaagcgg aaaaataacg gaggatcaaa tgattgatat gaattaccaa gtcaatgtcg
     3061 aaaagaaaaa gccgacagaa gtagcacgaa tcttcctgaa aaatgaagga ttagtaaagg
     3121 aggaagatca gtgactaaat taatcgagtt caaacatgtg caaaaaaagt atgatggaaa
     3181 gtatgtcatc gatgacctaa atctggcaat caaaaaagga gaaatctttg ttttggttgg
     3241 tccttccggt agtggaaaaa caactacttt gaaaatgatc aatggattat ctaaaccatc
     3301 agcaggagac atctacttca aaggaaaatc gttaaatgaa tataatctgc agaaaatgcg
     3361 gtggaatatg ggatatgtgc tgcagcaaat cgctttgttc ccgaccatga cagtcaagca
     3421 aaatattgaa gtcatccctg aaatgttagg ttgggaaaag cagaaaagag cagaccgtgt
     3481 agatgaactt cttcaaaaag ttggcttatc tcctgatatt tatcgagatc gtatgcctag
     3541 agagttgtct ggaggagaac agcaacggat cgggatcatc cgagctattg ctgcaagccc
     3601 agatgtgatt ttgatggacg agccatttag cgcattagat ccgatttcaa gaaactcatt
     3661 acaagaattg gttttgtcct tacacgaaga attgggcacg accatcgttt tcgttacgca
     3721 caatatggaa gaagcaatca agctaggtga tcgtattgca tttatgaagg atggagaaat
     3781 tatccaatgt gacacaccag agcagttatt gatgaatcct aaaaacgact acgttcggca
     3841 tttctttgat gaaccgaaac agacgaaaga gtggcgagta gaagatttag tcgtcaatgg
     3901 atatttctta aatgaaatac cagaaaatgc tcgtcaacaa gtttgctttg atacgcctat
     3961 gaaagaagta tattcgcttc tttcggtgta tccaagcatt gtgatagtag aaaagcaaag
     4021 aagtattggg tcgctgacaa gtaaagaaat ctttgctttt ttaagtcgag aggaggaagg
     4081 aaatgaaaac atatgatgca atcatcatcg gttcaggtgt gagcggctta agtgctgctt
     4141 atggattgaa agaagcaggt aaaacagtac tagtggtcga agaagatcta tggggcggga
     4201 cttgtcctaa ccgtggatgt gatcctaaaa aagtcttgtt aagtgcagta gaggcacgta
     4261 atcgggtaaa acaattgagt ggaaaaggtt ttaacgagat cccaactgcc aactgggaag
     4321 aacttcaaaa attcaaacga acatttacgg atccagtccc agaaagccga aaaaaacaat
     4381 tagcagaagc tgaaatcgat catctttcag gaaccgctcg ttttttggat gattcatcta
     4441 ttgaagtcaa cgaagaagtc ttccatgcgg attacttagt tttggcaacc gggcaaagac
     4501 ctacgatctt accagtggag ggaaaggaat acttgaaaac tagtgcagac tttttatcat
     4561 tgcctgtact gcctaaagaa atcattttta tcggtggtgg ttatattgct tttgagctag
     4621 ctacgattgc caatgctgca gggagcaaag taaccattgt ccatcataat caacggccat
     4681 taaaagaatt tgaggcgtct ttggtagagg aagccgtcca tcaaatggaa gcatctggca
     4741 ttcagtttgc ttttggtgtt gaaacccaaa agatcatttc agaaggtact cgctatcgtt
     4801 tagtcggaaa agagacggaa ttagtagcag atatgatttt ttgtgcgact gggagacagc
     4861 cgaatacaga atctttagca cttgagcaag cgaatatcgt ctttgataaa cacggaattg
     4921 cagtcaatga ttatctgcaa acgagcaatc caaaaatttt tgcgtgtgga gatattgttt
     4981 caaggaaaac cccaaaattg acaccagtcg ccacttttga aggaaattat gtagctaagc
     5041 ggataacgga tgcaacaagc gaacctatca aatatccgat tatacctacg atcgtctacg
     5101 ccagtcctaa acttgctgaa gtaggagtta caaaaagtca cgcctcttcg tcagatcaag
     5161 tggtagagat ggatcttacc agttggttca cctatcatcg tgtcaatgaa ccagttgcta
     5221 aggctgaatt gacctttgac cagcaaaatt atttgattgg tgctgcagtg attagcgagc
     5281 aagcagacga attgatcgat gatttgacac tcgtcatcaa tcaaaaattg actaaaaaag
     5341 agttggatag ctatatcatg gggtatccga cattggcaag tgatctctcc tatttattga
     5401 aataatcagc atgataacta aagcgcttgt tgctttttcc ttcttgtgct acactttaaa
     5461 aaatgaatac ctgtttctgg ggatgtcgta gacgacatga gagtaagttg tgaacttaga
     5521 cccattgaac ctgtcagtta atactggtga agggagaaac atagcagacg cttgaagcgg
     5581 gttactatgt cgtgcccatt tcaagcgttt tttgttattc atattagaag aaaggaggta
     5641 acaaaatgaa gaaaaatctt tatccagcag tcagtatggc attcatttta attatttggc
     5701 agattgttgt catagtttgg aaaatacctg cctttatttt acccggacct gctgctgtat
     5761 tttctgcatt agcggcagat gcacaagtat tactttccca ttctgtcacg acattaaatg
     5821 aagcagtgct tggtttggta atagctagcg ttctttcttt tattactgcc ttggcaatgg
     5881 attattggaa gtttttagaa atgtcttttt atccgttact agtgatttca caaatgttgc
     5941 caatcatggt gttagggcca ttacttaccc tatggtttgg tttcggtatg cttccaaaag
     6001 tgatccttgt tgtcttgata agctattttc cgatcgtagt ttctttttcg gatacattga
     6061 ggaaagtatc aaaagagcaa atcatctttt tgaaaacgat gggggcagat acagggaaga
     6121 tttatcgaat atataaaata ccagcgggtt tatccggctt cttttccggt ttaaaagtgg
     6181 ctgctactta ttgtgtcagc ggagcagtcg ttggagaatg gctaagcgct caagcaggct
     6241 tagggtatta catgatacgc gtaaaaaaca gctaccagat cgataaagtt tttgctgcga
     6301 tcatttgtgt cattctattg agtttattgc tgaatggcgc ttgttcatta ttgaaaaaag
     6361 ggtattatcg tatgctttat attttttaag agaaggggat caaatgaaaa aattatggct
     6421 tttattaccg ctactgttgc ttttatccgc ttgtggcact gcaaaagaaa caagtagcaa
     6481 acaagaaata aaggatttga aaaaagtgac acttgttttg gattatgtac cgaatactaa
     6541 tcatactggc atctacctag caaaagaaaa aggctattat aaagaggctg gtttaaatgt
     6601 ccaaatcatc gaaccaggag ataattcgac aagtattggt ctagtaggtg cggacaaagc
     6661 acaatttggt gtaagttatc aagaagatgt cacttatgct cacgcagatg gacagaatat
     6721 accagtcaaa gcaattgcta cagtgatcaa acataatact tctggattcg ccaccctatc
     6781 agacagtaac atccattcac caaaagattt tgaaggaaaa acatatgcag gttggcaaag
     6841 tccaagtgaa gaagctgtct taaaagctgt gatggaaaaa gacggcgggg acttttcaaa
     6901 actgacaatg gtaggaagca atggagaagg gccagaaagc ttaggaaaat cctcagatat
     6961 ccaatggtac tttgaaggtt gggatatgat caaagcaaaa gaagcgggta ttgaactaaa
     7021 ctatatccca ttgaaagaat tagatgaacg gttagattac tatactccgg taattattac
     7081 gaatgatcaa ctgatcaaaa gtgatccaga attggtacag tcttttatgg atgccacgaa
     7141 aaaaggctat caggaagcta ttaaggatcc aaatgacagt gctaaactgt tgcaaaaata
     7201 tgcaaaagaa aatgatcgga cgtttttaga agaatcacaa gcattcttgt caaaaaatta
     7261 tacggatgat cctgagaatt ggggattgat ggaagaaaaa gtttggcgca attatacagc
     7321 ttttatgcag gaaaacggat tgatcaagca agatgtacct tcgcaagaac tattcactaa
     7381 tcagtttata aaataggagt gatagatatg tcaatccaaa tcagccactt gaatgtagtc
     7441 attgaaaacc tgaaaatatt agaagacatc catttcacta tccctaaaga tacctttaca
     7501 tcgattgtcg cgccaagcgg agccggtaag tcaacattgc tgaaaacttt aacaggagtc
     7561 cagcctatga cttctggaac gatcaaagta gacgatcaaa aagtaactgg tctccataca
     7621 gcatttagtt atatgccgca agaagatatg ttacttcctt ggctgagcgt ctatcaaaac
     7681 gttacgttat atcaaaaaat caatcatcta acaatcaatg aagaacaagt aagggattat
     7741 ctggatatct ttgggttaaa cggctatgaa cattttttgc cggaacaatt atctggcggg
     7801 atgaagcagc gaacggcctt gttacgaaca atcatgaatc cttcttctta cttgcttctt
     7861 gatgaaccat ttggagcgtt agatgctatg accagagggc agatgcaaga ctggatgttg
     7921 aagctgccta aaaaagcaaa gcggactact ttacttgtga cgcatgatat cgaagaagca
     7981 atttatttat cagacaggat tcttgtgctt tccgcccgtc cggcacatgt gattgctgag
     8041 attcaagtgc ctgaaaaacg acggtcacgt gaatggctac tgcagcaatc agagttgaag
     8101 caaacgattt atcagttatt ggcaggtgaa ttagatgtta agtgatgctc attgtcattt
     8161 agatgggagt cagagtttag cactccttca gcaggaacac acgattttga cgatcatcaa
     8221 ttgtgattcg ccagaagaat ggaaggaaaa cagacagctt gcagcaagta agactcaagc
     8281 cttaagttac ggtatccatc cttggaaagc agatagctat acgtttgaac aagttgagcc
     8341 ttttttgaaa aaagcaagga tcatcggtga aatagggtta gataatattt ggacgaatgt
     8401 accgatgact acacaaaaaa aagtatttga gcgacagctt gcttttgcag ccataaatga
     8461 gaaacctgtt gtactgcaca caaaaggatg cgaaaaagag atcttaaatt atatccaagc
     8521 ttaccctaat cactatttga tccattggta ttcttctttg gactatcaac aagactatat
     8581 tgatttaggc tgttatttca gcattggtct tgatttgaaa aagaatccag cagtctggca
     8641 actagctaaa gcagtcccaa tcgaccactt acttattgaa acggatggac tacatgcagc
     8701 agagtgggct ttagggggga ttttttcaga aaaagattat gtctcactgc ttgaagaaca
     8761 gttaaagaca atcgctgaga tcaaagagct atcagttgaa gaagttaaaa aaaatactta
     8821 tcaaaacttg gaacgtttca ttagattagg taaataaaaa agagatgtga cacaactagt
     8881 gtcgcacctc tttttggctt tctttgtata aaagtaaact gtttgattaa taaatgaatc
     8941 ttagtaccaa ccgtttgctt cccagaatgc tttcgccgct tcccaagaac cataacgtga
     9001 agtaacatat tgttctgcta ctctttcttg gtttgcagca gagtagtcac catttaagta
     9061 agaagaatct aattggtaac gtccgatgta acgtccgttc gttgctgtgt aagaaccgct
     9121 tgattctttt tgtgcgatcc attcttttgc agagcttgtg cttgctggag tagcagcagt
     9181 ttctgttaca gcaggagcag gttgtgtttc agcaacaggc gctgcttgtt tttccgtttg
     9241 aaccgtttct tgtacaacag gttgttcagc ttggactggt gcttcttgaa cggctgcttg
     9301 aggttcagca gctgcttgtg cagagccttc tgttgggatt gttaattgtt gtcctgagta
//
LOCUS       DACDXO010000083         9281 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E23605_2017
            SAMN12639989-rid9881463.denovo.084, whole genome shotgun sequence.
ACCESSION   DACDXO010000083 DACDXO010000000
VERSION     DACDXO010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639989
            Sequence Read Archive: SRR10023389
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741914.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:42:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,972
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 33 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 17 of 73
            Pseudo Genes (multiple problems)  :: 10 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9281
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639989-rid9881463.denovo.084"
                     /isolate="E23605_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..435
                     /locus_tag="IWM35_002437"
     CDS             <1..435
                     /locus_tag="IWM35_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HAP9920262.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEPVQAEQPVVQET
                     VQTETQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQL
                     DSSYLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
     gene            complement(759..842)
                     /locus_tag="IWM35_002438"
     tRNA            complement(759..842)
                     /locus_tag="IWM35_002438"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(806..808),aa:Leu,seq:cag)
     gene            1370..1870
                     /locus_tag="IWM35_002439"
     CDS             1370..1870
                     /locus_tag="IWM35_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9920263.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
     gene            complement(2060..2404)
                     /locus_tag="IWM35_002440"
     CDS             complement(2060..2404)
                     /locus_tag="IWM35_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HAP9920264.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
     gene            complement(2558..3478)
                     /locus_tag="IWM35_002441"
     CDS             complement(2558..3478)
                     /locus_tag="IWM35_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP9920265.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            3665..4156
                     /locus_tag="IWM35_002442"
     CDS             3665..4156
                     /locus_tag="IWM35_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HAP9920266.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            complement(4180..6555)
                     /locus_tag="IWM35_002443"
     CDS             complement(4180..6555)
                     /locus_tag="IWM35_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HAP9920267.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            6881..7507
                     /locus_tag="IWM35_002444"
     CDS             6881..7507
                     /locus_tag="IWM35_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAP9920268.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            7530..8234
                     /locus_tag="IWM35_002445"
     CDS             7530..8234
                     /locus_tag="IWM35_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAP9920269.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            8426..9259
                     /locus_tag="IWM35_002446"
     CDS             8426..9259
                     /locus_tag="IWM35_002446"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HAP9920270.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
BASE COUNT         2828 a         1791 c         1651 g         3011 t
ORIGIN      
        1 tactcaggac aacaattaac aatcccaaca gaaggctctg cacaagcagc tgctgaacct
       61 caagcagccg ttcaagaagc accagtccaa gctgaacctg ttcaagctga acaacctgtt
      121 gtacaagaaa cggttcaaac ggaaacacaa gcagcgcctg ttgctgaaac acaacctgct
      181 cctgctgtaa cagaaactgc tgctactcca gcaagtacaa gctctgcaaa agaatggatc
      241 gcacaaaaag aatcaagcgg ttcttacaca gcaacgaacg gacgttacat cggccgttac
      301 caattagatt cttcttactt aaatggtgac tactctgctg caaaccaaga aagagtagca
      361 gaacaatatg ttacttcacg ttatggttct tgggaagcgg cgaaagcatt ctgggaagca
      421 aacggttggt actaagattc attatttgaa cgtttaaaca cattcaaaat atagtaagag
      481 gtcatgcgat ttattctttc gcatgacctc tttttgagtt taagtttagt ctttattcct
      541 taactatcgt ttgccagtat ttcttgtatc agaatctata aatctactat ttgaatcaat
      601 ctattaggtt ttaaactgtc ccaccttcac acaaattttt tttgatttat ttgaatctgg
      661 ttccgttgtt taaaattttt cctatattct tttagataat atgttatttt ctttctcatc
      721 gctccaggtg taccttctct ttataaaaaa acaaattatg cggctaagaa gactcgaact
      781 tccacgaggt caccctcact agcgcctgaa gctagcgcgt ctgccaattc cgccatagcc
      841 gcatcacatc ttttagttta ggattacatg tgttttttgt caagaacata tttttgttct
      901 ttttcatttc tgtacgccct cccttatgcc atctacacaa ataattcatc cgagcaactc
      961 catcatctat tgagggttgg tgaaaaaatc tgttttatcc atatgatcga tttcaatgtt
     1021 aaacgaacga ctctgggatt agtaactatt ttattttttt cttcatttct tttttcctct
     1081 ttttctcgat tcagctatct tctttcattt ggatattgat tatttcttct gttgattact
     1141 atccttctgg aattctagac gctgaagtaa ttcacgtttt ctttcttcta gagatcataa
     1201 gtaacgaatg aacgaacttc aaataatttt tttattgtaa ttcatatttg tattgttcga
     1261 tgattcttaa tacgatatga cacaataaat aaaaataggc atggctattt acaacaatag
     1321 tgctatagta gaaaacaaat gaataaggtt acttaaagag gtgaatacga tgaaactgat
     1381 caaactatgg gaagcagatt taaagaaagc ttatcagcta caaactagct ttggaccaaa
     1441 tgagagtgga tttatcaaca atgcttatgg ctatacattt gctgaatttt tagattatgt
     1501 aaaaaaatgc gaaaaaacgt ctttaggaat cgatcttcct gagagttacg ttcccgcaac
     1561 cgtctttatt ttagaaagtg atgaacatga gtatgtagga atttttaatt tgcgccataa
     1621 actgaacgaa acattaagaa acggcgctgg ccatattggt tacggcatct cccctaatta
     1681 tcgtaaaaaa ggatatgcca cacaaggatt agcattagta ctacaagagg caaaaaaaat
     1741 cggcattgat gaggcacttt tgtctgtaaa taaaaaaaat cttggcagtc ttaaagcaca
     1801 acaaaaaaat ggggctgcga tttatcgtga agatgagaaa gaatactata cgaaaatcaa
     1861 actgatgtaa aaaagaagga ttccaaaagg aaagggattt gaatgtcctt tatcaataga
     1921 acacatttat ttaattaact tataatatag cagacaaaat tttataaaca aagaaacctt
     1981 ttatctcttt tcttctagaa aggataaaag gcttttttgt ttttcctatt tgcttctcat
     2041 agctagtcag ccaagatttc tatcgtatcc ccaacgcaat ccgtgcataa cggctcatct
     2101 tatctgtcgt ccatgctgga taccagacta gtttgacttc tgctttcgtt acttctggca
     2161 cttcttttaa tgcgtcatgg atagattctg ttaaaatatc tgctaatgga cagcccatag
     2221 ttgttagagt catcttaatg actgtttctc cattttcagg attgaattct atatcataaa
     2281 tcaagcctaa attgacgatg tcgattccta attcaggatc aatcaccatt tccaaggcag
     2341 caagaatacg ttctttgatt agttctattt cttgtccaga ccgttcttgg tttgcttcgc
     2401 tcatcgtacg acactcctta acacgatttt tatttatcat actctttttt tagctgcttg
     2461 taaatgagaa tttagaagta agattctcgt ttaaatttat aaaaatgttc ttattagatg
     2521 taatagatta aatttattct aaaataaacc tgattcctca ttttttttgt gaacgatttt
     2581 ttatttctgg caatacgcca gttgttcgat acattttttg ttcgttttca tttgcaaacc
     2641 ggccaaatac tgaatgagtt ggtaaaatat tgataaatgc atcgtcgtcg atttcatgaa
     2701 cgatctgatc aagatcatac atttcgtagc gggtgatgac catcatgatc atgttccctt
     2761 gtttttttga atagccaccg acagacggca gtaatgtcat tccacgtacc atccgttttg
     2821 ctagttcttc tgttacctct tctggcttga cggtaacgat cattgctgtt actttttgat
     2881 gactcgtgtg gatggcatca attacttgag tcattgcata aatggaaata atcgtataca
     2941 aggcactttc ccagttaaag aagaagcctg cgatgactac aatgatacca tttagaatca
     3001 gcatgtagtt tccgactgtt tttccagttg tttgtgaaag tacaagagag atgatatcca
     3061 tcccaccagt tgtaaagccc atttttaaag ataatcctac gccaagacct aataaaacac
     3121 cgcctactag ggcattcatc aaaatattat cagtgatctg tccgactgga agcagcatcg
     3181 tcatcaccga tacacataca acattcacaa aactaaggat cgttgctcgt tttcctactt
     3241 tgagaaaccc taatacaaac acagggatat tcaaaagaaa aataaatgtt cctgtgtcta
     3301 tcttgatatg aaaccattca tataaaagcg aggaaatgat ttgagcgatc ccattcatcc
     3361 ctgcagaaaa gacattggct ggaatcaaaa aataatttaa agcgaccgca gcggtcaatc
     3421 cggttatcaa aataaccact attttcttta atctttcatt tttatgatac gcagtcataa
     3481 ttttactcac aagcacctct cccctacttt ggtattccaa atgtaagaat aacagtttat
     3541 ctattaaaat aaaatacttc gaactcaaat gagaagaatt ttagcaaaca ggtaagaatc
     3601 tgccctaatc ctatgataag atagatgtat ggtaaatatt tatatgtata tcaggagggt
     3661 tcctatggca aaagatgcta gcttcgacat tgtatcagaa gtcaatacag aagaagtaaa
     3721 aaatgcgatt cagcaaacaa caaaagaatt gtccaatcgc tttgacttca aggattctac
     3781 agtagaaatc aaattagaga acagcaatct agttgtattg agcgatgatg atttcaaaat
     3841 cgaacagatc aaagatgtat tatttggtaa gctgatcaaa agaaatgtgc caatcaaaaa
     3901 tatccatttt tccgaatcag aacacgctct aggtggcaaa gctcgacaaa ctgccgattt
     3961 ggtcaacgga atcgatcgtg aaaacgcgaa gaaaatcact acagctatca aaaatgaaaa
     4021 actgaaagta aaagcacaaa tccaagaaga tcagattaga gtgacaggta agaaccgtga
     4081 tgatctacaa gctgtgattg ctttattacg taaacttgac ttaccaatcg agttgcaatt
     4141 cactaactat cgttaatcaa aaaacgaggc tgacgtctgt cattcctcgt ttttttgatt
     4201 tactgcttct tttgataacc aaaaacgctg tattccttct tccaattccc caacacttcg
     4261 tacttctctt gctgtttgcc aatgttgtgg gaataaacgt cgataagaaa aactagaaaa
     4321 tcgttgatct gccaatacaa cgattccttg atccgccatg ccacgaatca ctcgaccacc
     4381 agcttgcaag actttgttca ttccaggaag ctgataagcg taagcaaatc caaggtgttc
     4441 cttctcatca taatatgatt tgatcagttc ctgctcatga ttcatctgcg gcaaacctac
     4501 acctacgatc atactgccga tcaatcggtc ccctctcaaa tcgattcctt ctgaaaaaat
     4561 cccgccaagt acgcaaaatc caatcaatgt ttcagcggga tttgtttgaa attttgttaa
     4621 aaatcgttcg cgttcttcct cattcatgtc cgtttcttgg attagcacat tgctgtcggg
     4681 atattgctgt ctgaatgctt ccaccgcaag atctaaatat tgataagaag gaaagaaaac
     4741 gaaataattc ccgacctttc catcagcaaa agcatgaatc gtctctacta attttgccag
     4801 actgtgttcc ctttttcgat aagtcgtctc taggtaattt gcgatgaaaa cttgctggtt
     4861 tccttctggg aaaggactcg gcagtttgta agccagactt ttttgtcccc ccagtgtttc
     4921 ctgataataa gagagtggtg aaaaactagc agaaaaaaga atactgcttt ttcctttatc
     4981 caacgactgc tcaagaaaca agcttggatc aatacaaaac tcttttacta tcagatcgcg
     5041 atacgttttt tcgactgttg tttcgtagtg atcatcataa aattcactga ccttcaaaaa
     5101 atgaagcaaa tcaaaataat aaggaagcaa ttgttcttgt gatgggtgct caggaaattc
     5161 tgccagccat tctttaattt tttcgcttaa ttgataacct gcctttacta aagattcagc
     5221 cggtgctttt tgatgatgat aatcccaatg atcttctttt gctatttctc ggatcgaatc
     5281 aaattctttt aatagtttgt tgaaacggcg atgaagtttt ttgaattcct ttggtatagc
     5341 ttccttactt cgtttcgttt tttcataaga tagttctgcc gaatacatct ctcgggaacg
     5401 attcaccaaa ttatgtgctt cgtcaatcaa aaacagatag tcctcgtttt tttcatcttc
     5461 aaaaaaacgt cttaaataaa cagttggatc aaataaataa ttgtaatccc caatgattac
     5521 gtcacaccag atactgacat ctaatgatag ttcgaaagga caaagtgtat gctttcttgc
     5581 ataagtttcg atgacttcac gagtgatctg attctcatga ttcaaaagat cccataaacc
     5641 ttcattaatc cgattgtaat aaccattggc atatgggcac tgatctggat tacaggtcgt
     5701 ctcatctaaa aagcagatct tgtctttcgc cgtcaaagtg acacttttta cttcagctcc
     5761 tgtatctttt aatgctgtca tagcatcttc agctacttgt ctagttattg ttttagccgt
     5821 tagataaaag atcttttctc cctcttcttc ccctacagct ttcaaagcgg ggaataacgt
     5881 agagattgtt tttcctgttc cggtaggtgc ttctacaaac aatttctgtt tcgtacggat
     5941 cgttttataa gcagctaccg ctagttcacg ctgacctttc ctatactcat catgggggaa
     6001 tttcaaagcc atcaaagagg cattgcgtac agtgcgccag ttttcttgaa aaacaagcca
     6061 ttctttataa tcttcagtca gctttttaaa aaaagtttct aactgctctc ttgtctgatg
     6121 ttcaaccttt ctcgtgatca cttcttctgc cgtctggaag taagtcaatt gcagatttat
     6181 ttcttgtaaa tcatgttctt gactatagat ataggcatac acccttgctt gatgaaagaa
     6241 aagttccact tgttctggtt ctaaatcttc aaatcgtggt tcggaggttt tgatttcatc
     6301 tatataataa ataccgtctc ttttgaaaat accgtctgca cggccttcaa cgatcaggta
     6361 atattcatct aattgaactg ttgtttttaa aaagacttct ttttcatagt tttctcccgc
     6421 atctttttgg agcttgcggt ggattctcgc tccttctaat gcagtatggt tacttttttt
     6481 acgattgtcg atacttccac gtcgcaaaat aaattctact aagtgccgca cagatatttt
     6541 tgactgtttg atcatttcca ccacctcttt ctctctaaga ttacctgaaa aaacctagtt
     6601 tagcaaaaag agctctcttt ccaaacataa aaaaacaccc tgctgattag gcagggatat
     6661 aggatataga atttggattt ttggatgaat ctaatgtaac aaggacatat taaaatgcta
     6721 tgaaaactta ctttccttct ctttagaata tccaaatact tttcatatag tctgataatt
     6781 cagataagta aagagaaata agtacctgtt tttgtttcct agcaatgaat gcgttatact
     6841 aatggatgaa agaagttttg gctatcaagg aggaatgcac atgcatatcg aaagattaaa
     6901 aacaggaatg attgaagaaa actgttattt ggtctacaac gaagaagcac ttttgatcat
     6961 tgaccctgga gaagatgcgg aacgaatcaa acaagaaatc aaaaaaacaa accaacagcc
     7021 ggtagcgatt ttgcttaccc atactcacta cgatcatatc ggtgcagtgg aacctttacg
     7081 cgcgtattat caaatcccag tttatgtcag cccactagag caaaaatggc taggtgatcc
     7141 gattttgaat ctttctggac tcggtagaca tgatgatatt gctgatgtta ttgtctcgcc
     7201 tgcagaatat gaatttgaaa tgaaaactta tactttagga aacatgtctt ttcgcgtcgt
     7261 ccctacccca gggcattcta taggaagcgt cagctttatt tttgatgatt ttgtcgtttc
     7321 tggcgacgcc ctgtttagag gaagtatcgg tagaaccgat ctgtatacag ggaatttaga
     7381 acaattactt catagtattc ggacccagct gtttgtatta cctgatgaat ttgcagtcta
     7441 tccaggacat ggcgatgcaa cgacgattga acaggaaaaa aggacaaatc cttttttcaa
     7501 tcgttaaaac gacttaggag gagaataaaa tgacagaaac tacactttat atcgtacgac
     7561 acggaaaaac gatgttcaat acaattgaac gagtacaagg ctggtgtgat acacccctca
     7621 caaagcaagg acaagaaggc attcattatt taggtcgcgg gttaaaaaat gtcgactttt
     7681 catttgctta ttctagtgac agtggtcgtg caatcgaaac tgcccgtatt attctaggag
     7741 aacattctaa gggaaaagaa attccttatt ttattgaaaa aaggattaga gaatggtgtt
     7801 tcgggtctct tgaaggtgga tatgacatgg aaatgtgggg tgtgatccct cgtgtattga
     7861 attttaaaac atatgatgaa atgtttacca cagaagtttc ttttgaacaa attgctaatg
     7921 ctgtatacaa cttagatacc gctgggtggg cggaaactta tgaaacattg cgagaccgtg
     7981 tatggacagg atttgaagat atcgcccatc actgcgaaaa aaatggtggt ggaaatgttt
     8041 tagtcgtatc tcatggtttg acgatcgcct ttttgcttaa tctgatcgat cctaaacagc
     8101 ctgttcgtgc tgggctttta aatggcagcg taacaaaggt cacttatgca aacggtcgat
     8161 tttctatcca cggcatcaat gatacgtctt atatcgataa cgggaaaaca catagtcgcg
     8221 actcgtttct ataatgggga tattttaatt tttaccagag aatcataaaa ggcaaagtct
     8281 gaacgagggc tttgtcttct tttttctctt cgtgtactta tctgctttat ttctcaagtt
     8341 tttcttaata ttaaaaaaca gattgggttt ctcaaaaaat aacggtatac tataagatgt
     8401 atcttaattg ggaaggaagg aatttatgtt ttttgcaaat attatcaaag cagtgattct
     8461 cggtattatt gaaggaataa cggaatggct gcccatcagt agtactggtc atttgatcct
     8521 agcagatgaa ttcatcaaac tggatgccag ttcacaattt atgtcgatgt tcaatgtcgt
     8581 tatccaatta ggggcgatct tggctgtcgt cgttctttat tttcacaagt taaacccatt
     8641 ttctccaagt aaatcaacgg tagaaaaaaa agatacgtgg acactttggt ttaaagtgat
     8701 cgttgcttgc ataccagcca tcatcattgg tttgcctttg gatgattggt tagaagcaaa
     8761 tttccacaag ttcttaccag ttgccattgt tttgatcgtt tacggtattg cctttattat
     8821 tgtcgaaaaa cgcaacaaaa acaaaacacc tcgttggact tctctgaatg actttacgta
     8881 ccaagcagct cttcttgttg ggttattcca agtcttggca cttgttcccg gaacatcccg
     8941 ttctggcgca acgatcctag gatctatctt gattggtgct tcgcgttttg tcgctacaga
     9001 gttttcattt ttcttaggga ttcctgtcat gtttggcgca agcggcttga agatcgtcaa
     9061 atatttagct gaagggaatt cattcaaaat ggaagaaact gttgttcttc ttgtaggaac
     9121 attcgtatct tttgtggttt ccgtcttagc tatcaaattc ttactaagct atttgaagaa
     9181 aaacgacttt accgcttttg gctggtatcg tattatcctt ggtatcattt tgattggtta
     9241 ttggtttatt gcaatgtaga aaaaaccaga acacttctct t
//
LOCUS       DACDXO010000084         8904 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E23605_2017
            SAMN12639989-rid9881463.denovo.085, whole genome shotgun sequence.
ACCESSION   DACDXO010000084 DACDXO010000000
VERSION     DACDXO010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639989
            Sequence Read Archive: SRR10023389
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8904)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8904)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741914.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:42:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,972
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 33 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 17 of 73
            Pseudo Genes (multiple problems)  :: 10 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8904
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639989-rid9881463.denovo.085"
                     /isolate="E23605_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(305..661)
                     /locus_tag="IWM35_002447"
     CDS             complement(305..661)
                     /locus_tag="IWM35_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HAP9920271.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
     gene            complement(675..1085)
                     /locus_tag="IWM35_002448"
     CDS             complement(675..1085)
                     /locus_tag="IWM35_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAP9920272.1"
                     /translation="MIKVYTSPSCTSCRKARAWLQANQLEFEEKNIFAEPLTESEIKK
                     ILAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
     gene            complement(1432..3618)
                     /locus_tag="IWM35_002449"
     CDS             complement(1432..3618)
                     /locus_tag="IWM35_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAP9920273.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNIAVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
     gene            3836..4519
                     /locus_tag="IWM35_002450"
     CDS             3836..4519
                     /locus_tag="IWM35_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HAP9920274.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            complement(4600..5319)
                     /locus_tag="IWM35_002451"
     CDS             complement(4600..5319)
                     /locus_tag="IWM35_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAP9920275.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTNK"
     gene            complement(5631..5837)
                     /locus_tag="IWM35_002452"
     CDS             complement(5631..5837)
                     /locus_tag="IWM35_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9920276.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            6082..6684
                     /locus_tag="IWM35_002453"
     CDS             6082..6684
                     /locus_tag="IWM35_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory regulator AgrB"
                     /protein_id="HAP9920277.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFALEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            6825..8105
                     /locus_tag="IWM35_002454"
     CDS             6825..8105
                     /locus_tag="IWM35_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP9920278.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIDDFFVLKS
                     SVIEPGDSLQYFHIVLSAFLSALLAFCLKKSLETWFHTVDRRMIGTIGTLILFTYYII
                     IFYTRFSGETPDVLALNTSFFILYLCVGLIILVYYWKISNKKLADQLLEQQVRMQQDY
                     IKDLEKNYQDLREFKHDYQNLLFSLHSYISEGDLEGLRSYYDQTILPTRQMMNLFPAN
                     LSMLDNMKVPEIRSLLSLKLMMAQEKGLIVQLHFPEPIELDTKHTVNLVRMLGIILDN
                     AIEGASAAKKKKIELTILKKNHSVMIRVINTTTNTLPLNQLKQKGFSTKTNPKNEGLG
                     LFILDELTKTNPYLFLETSIENQRFSQTIYIQIK"
     gene            8118..8846
                     /locus_tag="IWM35_002455"
     CDS             8118..8846
                     /locus_tag="IWM35_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9920279.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
BASE COUNT         2587 a         1667 c         1463 g         3187 t
ORIGIN      
        1 actcagttat cttatcatgt catttctaac atgtcaacaa ctattttata tgttttttaa
       61 aatactattt tattgctttg ctacttaaaa caacagaaat gaatatatca tgtaaattta
      121 cacatgtcaa tatattttta actctatatc cccacctaga tttgaatttt gaccacataa
      181 attgagcatg tctctatagt agaaaaataa agtcaaaaaa agaacacata acacgcttga
      241 tgctaagtgt tcgactgtta caactgtatg aaatgctcag tgacttgatt aaaacagtta
      301 aagtctattt atttaagata agctgttcca tcaactgctc attgtatcca ataaatagaa
      361 cttcatcgat aataatcaaa ggacgtctga ggagattggt atgttgtgtg cataaaaaaa
      421 tcagttgttt ttttcctaca caggaaaggt caatcgcaat gttgtgaaaa gcctcagacc
      481 aagtcgcaaa tagctgttct gcttgcactt cttcaaaact gcataattct tctgcttctt
      541 ctgtacttag atcgcgttct tctaagtcaa caaattgata aggcaattga tggtgttcta
      601 accattgttt cgccttcaga cttgttacac agtgcgctga tccatagact ttcacttcca
      661 cgaaggctcc ctccctatga atttaagatg ttattgtttt gtaaatagat cagatttctg
      721 gcaacacatc gaatttcacg agggacaaat gaccggatct catcttcatt aaatccgatt
      781 agtattcttt tttcatcaat caaaataggt cgtctcaata atgaaggata ttctttgaat
      841 aagacgatca tttgctttaa agttaattcg ttaaaatcta tgcccagctt ttcgtaaatt
      901 ttcgaccgag tggaaataat atcttgaact cctccttcag tcgctgccaa gatctttttg
      961 atctcacttt ctgttaacgg ttcggcaaaa atattcttct cttcaaattc aagttgattc
     1021 gcttgcaacc atgctcttgc ttttctacag gaagtacagc taggcgaagt atatactttt
     1081 atcattttct cactcatttt taattataat ccaattctac agtaatttct tttaaaatac
     1141 caattattag ctaataatat ataaaaaaaa atcaaaataa ctgattattt acgtttgaat
     1201 aatgagaatt tttctatata ttctctattt tgataatagt attctcattt tcaaaataaa
     1261 taagggaggt gtgacaaaat tcttgtcaca cctccctttt ccgaataaac tgtgttccag
     1321 aaatagcttc ttcgaaaata agctgaaatt tcacaaaaat ttgagaagca attttcgtac
     1381 attccttctt ctttcttgaa actgaccact tctgtcacac cctctcttct atcactttcc
     1441 ttttttcaca cgactcccgg tttctttgat aaatctcgat actttcaatt ttcttgaatt
     1501 tttcatttga ggtacacata gatacagacg atttcttgct ctagtcatcg caacataaaa
     1561 tagtcttcgt tcttcttcta tcgtcgtcac tggttctgct tttatttcat taaagtcgat
     1621 tactttcttt tctgtcgctc cgttataagg caaacttcct tcgttacaac caatcaaaaa
     1681 gacatttcgg taactaagac ctttactttt gtgaatgctc tggatacata gtgcatcaag
     1741 ttttccttgt ctgtaagcaa tcaagcgggt ttttaattct ttagctgtcg cttgggtttc
     1801 ttgcgataag gcttggagtc ttctgaaagt agcatgctgc tttacttctt ctcgtatcca
     1861 ttcttgatca ctctggtcga tttccttagc tatacgtggc agatgaagat agctttgaac
     1921 aagcgaatag atttcttgat gctggtctgt tttacgcaac gcttcctcaa actctcggat
     1981 ttctgcagat ttcgatttta aattggggct tatcgacaaa atataatctg caacggctat
     2041 attttgatat ttaggagaaa agtattgatc gcattcactt tttttcaggt agcagtctgc
     2101 catcagctgc cggagcaatt gctggtaggc ctgatttttc ttttgcagat tcagtgctgt
     2161 ttgccaatga acaatttttt gatagaaatg ttggaaagac ttgataccaa agataccttc
     2221 atatggacta tctgcttcca gtacaaaagg aatattcgtt ttcagcagag attggacaag
     2281 ctgctgcgtt tgtgaagctg atcggtataa tatgattgat tccttatatt tcccattttt
     2341 ttgaataaat gtctgaattt gtttgattag ccactgactt tctgctttag tatcagaaaa
     2401 caaataggct tccataacac ctgcttcttt ttgtgctgga atcatcgcct ttggtatccg
     2461 atcattttta ttatagctga tcaatctatt tgctccttgg acaatagctg cccgagaacg
     2521 ataattagtc aataagaaaa gagttttggt aggatattct ttttcaaagt tcttgatgta
     2581 tgctggctct gatcctctga atccatagat actctgatca tcatctccaa caacaatcaa
     2641 cttgatcctg ttttcctttg tcatccgttt cacgatttcc cattgaagtg gattagtatc
     2701 ttgaaattca tcaatgaata gataatcata tttttggccg atataatgct ggatctcctc
     2761 attctccaaa gcgcttttca gattttggag tacatcattg aactctagca attgctggct
     2821 tcttttttgt tcttggagtt ccgtgagcaa tccatacagt cgttctgata tcggctgtcg
     2881 tgcattctta tcaatggaat catatttttc tttaactaac tgaaccattt cttctactga
     2941 aaggttcata ttctgccaat agtctaaccg agaagcaatc gttttttctc catattgatc
     3001 aaatatataa agattatatt tacgtatcag ctgttgcaaa acagcattca ctttttgtgc
     3061 agtatccttt gaaaagtctc cttgtattcc ttgcattgct acttcaggaa attttcgaca
     3121 aatttcattg tatatcctaa aaaagatact atggaatgtt ccgatatgga cagcggtttt
     3181 tctctggctg aaattatcta ctcgtttttt tagttcattt gcagcgttcc ttgtaaaagt
     3241 gatcattagt atgcgttctt cgtctacttg tttttgcgtc atcagataaa cagcttttgc
     3301 acagattgtt tgcgttttgc cgctcccagc tcctgctatg attcgaagat gtttcttcat
     3361 atcatatgtg atagctgctt tttgttcttg attgaagcga ttataggaac gtttctccat
     3421 cattttaaag aaagagctgt ttccttcact ttcttttact tgtacttctt cctcaataag
     3481 tccgggtgct tcgccataag ccgcttcata ggtagcaatg atttcttctt gtagccgaaa
     3541 gacttgttga agctttttat acttgttctc ccagcgtttc aattcttttt gtaatcggag
     3601 aatttctttt tcattcaact gattgatttc cttcccatct ttcttctttg aatttgttta
     3661 tttcaagttg gttatttaaa gtttcgttgt ctattttttc tttatactaa cagacctttc
     3721 taaaaatgaa aatgggaagc gatatgattt tagttttctg aaaatttttc ctttttttca
     3781 atcgtttatg ctaagataag gaagacaaaa atattcataa aaggtgatga aaaaaatgca
     3841 gacttccttt cacgaggggc gtacaatagg acttttgctg acatttatcg gaggagccat
     3901 ggacagttat acttatatcc aatatggtgc ttttgcctct gctcaaactg gcaatatcat
     3961 tctatcgatc atccaagcat ttgacggaga atggctaagt gttggtaaaa aagtattgtc
     4021 tacccttttc tttttcttag ggattcttct aacaaagtat ctaattgatt attttagaaa
     4081 aaaagaaaaa catttctggc gcttgtttgt tctatattat gaagcagtga tttttttctt
     4141 tgttagtttg acacccgtcc atgagcatcc cgcattagta acgattttga ttgcttttac
     4201 tgcatcgatc cagtgggtag cttttgataa aatcaacggg cgtgcctaca ctaatttatt
     4261 tacaactgga aatttaaaag gggtagcaac gaacttatat gattattgta tttctaaaga
     4321 aaaagcagat tttgatcgat ttttccacta tctaagtgtc gttcttgcct ttatcgcagg
     4381 agctattgtc cttgttttct gtcatcatct gtttggcgcc cattcgattt tgatcgtttc
     4441 cattttattt ttgatcatgg ccctcactca gacatttcat ttatggcaat tttatcatac
     4501 gaatcggttc actatttgat ctcaatgaat caaaaaaact aagcaagatg ctgctcacag
     4561 agaaatatct gcgagtgagc aatttgctta gtttttatat tatttatttg taaagattcc
     4621 ctttgctttc gggttatttg tagtgtagcg gatcgactgg gcatctgcct cttctgtcat
     4681 tttctttgcc cgcatcttga tttcttgtac gatttgttct ccacatgccg gatcggctac
     4741 gaaatcatat ttgtccccat cgatcactat tttgggacat atatcaaact gttcatacca
     4801 ctcttcgtat cgtcgattga gttctttata gtaggaatag agttcaggat cttgttctag
     4861 ctgttcatat tcccggcctc tttttttgat tctcgctaac atagtttcaa aggatacacg
     4921 aatataaacc agtagatctg gtttcttttg tggcgaaatt tcttctaact ccttcagcat
     4981 cgtttctaaa agaccttcat actgctttac ttctacttca gaaacacgcc ctaaatctgc
     5041 attcaaatga aacagcatac tatcttcgta gatggagcgg tctagtacgt tgtcatcatg
     5101 gctgaaagca tctttgattg ccaaaaagcg tttgttcaaa aagaaaatct gtaataaaaa
     5161 agtatacttt tctggattag aataaaatag cggcaatact tcattatctt ccacgttctc
     5221 ataaaaaggt tttgtttcta aatcttgggc aagcatttca gttagtgtgc ttttccctgc
     5281 accgatcgtt cctgcaacaa tgatcacaat tatcggcatc cttcctgtaa taaaatctgt
     5341 tcttcatttt actacttcat ttatagaaat aaaagccttg actttatctg ctccttaaaa
     5401 gctacttctc taaaagtagc caaaagtaac tcctattcat gaaaataaag gctgtgacaa
     5461 aagtcttgtc gcagcccttt tttcaaataa acagtattct cctgccaaca cctcggtcac
     5521 aaggcaatat tcataaaaca tgaaaagctg ttttttaaca ttattccttg taactcagag
     5581 ctaaacggga gactcacaac ctctttatgc tctcaacatt ttatctgttt ttattcttct
     5641 attgtacttg atttgcgtac aactaactgc cagtgcagat tatgagcggt atcattgtct
     5701 tctactgcgc atttcatgac acgtatcatc acactatttt catagatttt ttctattttt
     5761 ccttttattg gtgattcaaa taaatcagat ttaaatgcat atgtttcgcc gacttcaaac
     5821 aattgttcat tttccataag aatccctcca tcacctttta taaggtcagt aattttttaa
     5881 cataaatcac tcgaaaaagc acaaacttgt cataaaaagt acttttcgta tcaaaactgt
     5941 cacttttttt ggtaaggttc ttggaaaacg aagatttttt taggagatca tgacggtttt
     6001 gatgtcaaaa ccacttttta tgaaacaaaa gacgtttttt atttttttta gcataaaatt
     6061 tgtggtggag gtacgtatga gatgaaagat aaagttgtga acaaattatt agaagcctta
     6121 ttcagcggag acaaatcgga agaagatatg gtgtatgtcc aagtaaaatt tgccttagaa
     6181 gttttattga ataatcttgg aaaactagcg gtcgttctag tcttttctct ggtgacaggc
     6241 tcatgggcgg aaacaggaat cacctttctt tcttatatct gcatccgcag atatgcttat
     6301 ggattgcatt ccgactctga attcgtttgt ctgctgtgga cactacttta tttatggggc
     6361 gttcccttgg tgatgaaaca tctgcaattg actatctcct tcccaatgat ggtctttttg
     6421 ttaatcagct gctttttgtt actgcttcgc tatggatcaa gaggtacagc aatcaatccc
     6481 attgaacccg agaaacgtcc cccattatta aagaaggcga tcagcatgtt tctaattttt
     6541 tctctcatta ctttattttt ctcagcttct tacttttcta cctatttgct acttgggatt
     6601 gttttagaaa tagctacgtt gcttcctatt acaaactact tgatgaattt tggaggaatc
     6661 tttcatgaaa aaaacaatca ttaaacaatt aactaactta tctgactttt ttgtatctgg
     6721 ctgctgcttt ggtatgagtt ctgaattcaa aatccctgaa aaattaaaaa aataatacgc
     6781 taattgtgct ataatttggt aaaaatcaat gggagctgta ctttatgttt ttatggtcca
     6841 tagcgttgca atatttgatt tacacaattt gtatcttatt gattgaccat cacatccgat
     6901 acaaatatct gtttgcggga gtcattctga tttcttgtat tttgattgga tgggtctatt
     6961 cgattttagg gatttttact gctctaattg ctataggaat gctgatgctt acttttttct
     7021 tcttttcgac ttcgcgtcaa caattggttt acgcgcttcc ttttgggtta ttgacgtcgc
     7081 ttttaggaga tcacttaaca agcatcgacg atttttttgt tttgaaatct tcggttattg
     7141 aaccaggaga ttcattgcag tattttcaca ttgtcttatc cgctttttta tctgctttat
     7201 tagctttttg tttaaaaaaa tcgttagaga cttggtttca cactgtggac cgccgtatga
     7261 tcggtacgat cgggacattg atattattta catactatat cattattttt tatacccgtt
     7321 tttctggtga aacacctgat gtcttagctt tgaatacgag ttttttcatt ctatatctgt
     7381 gtgttggcct gatcatcttg gtttattatt ggaaaatctc taataagaaa ttagctgatc
     7441 agttattaga gcaacaagtc aggatgcaac aggattatat caaagatcta gaaaaaaact
     7501 atcaagactt gagagaattc aaacatgatt accaaaatct gcttttttct cttcacagct
     7561 atatttcaga aggcgactta gaaggattac ggtcttatta tgatcaaacg atattaccta
     7621 cacgccagat gatgaacctt tttccggcca atctaagtat gttggataat atgaaagttc
     7681 ctgagatccg tagtttactg tcattaaaac tcatgatggc acaggaaaaa gggttgatcg
     7741 tccagcttca ttttcctgag ccaatcgaac tggatacgaa acatacggta aaccttgtac
     7801 gtatgcttgg tattatcttg gacaatgcga ttgaaggagc tagtgctgca aagaagaaaa
     7861 aaatcgaatt gaccatactc aagaagaatc attccgtgat gattcgtgtg atcaatacaa
     7921 caacaaatac attaccgtta aaccagttga aacaaaaagg gttctcaaca aaaacaaatc
     7981 caaaaaacga aggccttggc ttgtttatct tggatgaatt gacaaagacc aatccttatc
     8041 ttttccttga aacatcaatt gaaaaccagc gtttttctca aacgatttat atacaaataa
     8101 aataaaggag taatgatatg attcccattt atatttgtga agacgatcca aaacaattag
     8161 aagttttatc aacggtgatc aaaaatagaa tcatgattga aaacttagac agttatgtcc
     8221 atatttctac ctctgatccg gaagaacttc tcaatgcagc caagatcaga acttcttctt
     8281 ttggtattta ctttttagat atcgaactag aagacagtga aatggatggt gtagcgatcg
     8341 gtaaattgat ccgtgaatta gatcctttag gaaaaataat ctatgtgact tcccatacgg
     8401 aaatgtcgat gaaaatctta aaaagcaaca tcgaaccgac tgattatatc gtcaaagaag
     8461 atctttatga tttaaaagaa aatgtcgagc atatcctgag caaaattttc gaagattttc
     8521 aaatgtccca aatccaaaaa gatatcttca aaatagagtt taatgatgag atcaaatttt
     8581 taccgatcaa agacatccaa tatttttcta cttcgctagg agcgccacat aaactggaag
     8641 tccatttgac tcatgcccaa ttacaattct atgagaagat caaagaaatc gagaaaatgc
     8701 atcggaattt tatccgttgt cataaatcct atgttatcaa tacacaaaat gtgcggtcga
     8761 ttaataaaaa aaccagagaa gtcacgttgg caaacggcga gaccatccca gcctctatcc
     8821 gtggattaaa gaaattgatt tcataaaaag aaccgaatag tttggaccga ctaaatgacc
     8881 agccgatcca aacccttcgg ttct
//
LOCUS       DACDXO010000085         8675 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E23605_2017
            SAMN12639989-rid9881463.denovo.086, whole genome shotgun sequence.
ACCESSION   DACDXO010000085 DACDXO010000000
VERSION     DACDXO010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639989
            Sequence Read Archive: SRR10023389
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741914.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:42:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,972
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 33 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 17 of 73
            Pseudo Genes (multiple problems)  :: 10 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8675
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639989-rid9881463.denovo.086"
                     /isolate="E23605_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..1101
                     /locus_tag="IWM35_002456"
     CDS             <1..1101
                     /locus_tag="IWM35_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAP9920280.1"
                     /translation="KKETIVRFEQYNHMPLVTRLKKQRYTCKNCRTHWTAQSYFVRLS
                     HSIADHVRYKIASLLTEKVSLSFIAKSCQVSLTTVTRTLKEFKSYLPKPSKKILPRVL
                     MVDEFRSHASIEDKMSFICADGETGNLIAVLPTRKLPRLTSYFLGCTNPEEVEFLVTD
                     MNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQALNELRIREMNELRKAGQKSQAEKLKK
                     NWRFLLKNRASINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSAPLKEAYPFFHELV
                     EAFRDKDPDLFFSLLAELPETLDDGFREKLQNLLTYEEGITSAMIYPYSNGKIEAKNT
                     HIKTMKRVSYGFKSFENMRIRIFLINQLINVR"
     gene            complement(1280..1615)
                     /locus_tag="IWM35_002457"
     CDS             complement(1280..1615)
                     /locus_tag="IWM35_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAP9920281.1"
                     /translation="MKMNTNDIATVYVAFTAAEGGKRRPVYILKNGLDTISFLSITSK
                     YHTKSDKIKLKYVEIRDWKQAGLKKESWIDVGTLNELPKNNKKVKWKKIGELSPSDLQ
                     RLEERMEEL"
     gene            complement(1599..1871)
                     /locus_tag="IWM35_002458"
     CDS             complement(1599..1871)
                     /locus_tag="IWM35_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HAP9920282.1"
                     /translation="MEKMAKLQISMDEKLKNEAESIIDELGMSPKTAVTVFYKQIVEQ
                     GKIPFSIELSEKSKRIHNIQRLAEKLPVQKLDTDEKIEEWFNEDEY"
     gene            complement(2322..3125)
                     /locus_tag="IWM35_002459"
     CDS             complement(2322..3125)
                     /locus_tag="IWM35_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HAP9920283.1"
                     /translation="MRFLHLSDIHFRENYQTNLFTAELFPTIKNPQLALEKLLQELSL
                     TVYDFILLTGDLVHEGGISDYQVLRKMLDQYFPTTPYYFCRGNHDRRSVFFEGMSVES
                     NETGEYIHCTNQNGLRIISLDSAQDTQHEGKISIGQMIQLQEWLGTPAEKGTILLVHH
                     PLSWEDPSIVTEVPEGFAELIAESDILGIFVGHIHQGSSAEFAGKKQFMAESLSFGVD
                     EYPDYSLFTNRTGFTSGSLIDGKIFTYQHYLTPHQIEIGRMKKPITGNH"
     gene            complement(3103..4227)
                     /locus_tag="IWM35_002460"
     CDS             complement(3103..4227)
                     /locus_tag="IWM35_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9920284.1"
                     /translation="MGETLELRNIGKKYGEHTILKEIDLAVAAGERIVLLGPSGSGKS
                     TLLRMIAGLEKITSGELFLGTQLANKLPCGEREIAMVFQNYALYPHMTVKENIIFALR
                     ANKIKTADIQHRLEEALKMLGLTPYQDRLPKDLSGGQRQRVALARAVVKRSNYFLLDE
                     PLSNLDVRLRLDARKELVKIHQRYKQTFVYVTHDQIEAMTLADRIVLLNEGRIQMIDT
                     PKNVYTKPKNTFTANFIGSPGMNIMPVTYDQGTLKVASQFLTLNPAWQKQLLQVSGSL
                     YLGIRPEHVTFSKETSDLHGQVKYRELLGQSYAYTVKVDGIEMIVLNEKPTWQIGDAV
                     NLRIEPERMHFFDYQTQENLGYPDYFIEERGNSHALFASL"
     gene            complement(4243..5058)
                     /locus_tag="IWM35_002461"
     CDS             complement(4243..5058)
                     /locus_tag="IWM35_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006739361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP9920285.1"
                     /translation="MKIKRWLPHLLFMFIIIIIIYPLLFIMANSFKPLKDAYQTITSL
                     IPQPFTLENFCYLFENLPIIKITRNTFFIATIITVVKLGVAFFAAYSFTYCRIKGKKI
                     WYFIIIGTMFIPFTVAMIPNYLLIAKLNLIDTTWGVIIPQFADAMGIFLLTQTMREIP
                     VSLLDVAALDNIKQTTVMSKIVFPLTRHAITSTGIWFFITSWNEYVWPVLILRTVDNY
                     TLPLAMQTYISSEGGTNFTVAMAISLITMFVPLVLYAIFQKYIIGTFVSAGIK"
     gene            complement(5048..5929)
                     /locus_tag="IWM35_002462"
     CDS             complement(5048..5929)
                     /locus_tag="IWM35_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006739050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP9920286.1"
                     /translation="MSEMKRYVGYLYLAPAIILLGVFVFYPLIYTIYLSFFDWNMVAP
                     TKEFVGFANYVKVLTDPVFHKVLKNTLSYTVLFVLLSCVFPYITSFLLGIVIEKGQNL
                     YKPLFFIPAVISLVVAGMVFTWILNPVSGPIAVLLGNFGLRLPMWMNLQGWVIAVIAG
                     ITAWKVFGYNFIVIFAAIVGIDQEMIEAAKLDRVPIWRIFFTIVLPMSSSTGLYLLVM
                     TIVQGLQYVFTPIKIITRGGPNNGSANVIYYAYQKAFEMYKVGESSAISVLTLLIFAV
                     FLGVIFKFVEKGVYYEN"
     gene            complement(6005..7294)
                     /locus_tag="IWM35_002463"
     CDS             complement(6005..7294)
                     /locus_tag="IWM35_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002581297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP9920287.1"
                     /translation="MLKKNLVIGVVSVLSVISLAACGSGNTKTTKKSEGRVTIEYWHV
                     NADTLGGKTVNELVDKYNQSQDKVKVVAKYNPDMYKGLMQNLQASVASKEIPDVVQVG
                     WAFKDYFAENFKYTNPQSLVEEVDPDNADYFKDHFLDNIMALSEKDGKQVGIPYSISN
                     PVLYLNQDILKAAGLDENGPKTWEEVQTFSKIIKEKTGKYGVYIQEPADNWAQQAILE
                     SNGASVIKDGKATFASKDGIAAYELYQRMVKDQTALHTTWEQGVQSFIDGNVAMVYTT
                     IAQRDNIQKNSTFTAKAVESPSWEGKKVKLPAGGAMLAVTSQTDKKKEATWDFLKYLY
                     SVESLAKWTEGTGYLPPRKDVADAQNGLKAFLAENQMMIPAIDQMDKMVSWSSFPGDA
                     GLEAEQKLLDMRDKILSGNSVNETMKATQKEINELLK"
     gene            complement(7522..8166)
                     /locus_tag="IWM35_002464"
                     /pseudo
     CDS             complement(7522..8166)
                     /locus_tag="IWM35_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303552.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
BASE COUNT         2691 a         1850 c         1371 g         2763 t
ORIGIN      
        1 aagaaggaaa cgattgtccg ttttgaacaa tacaatcata tgcctttggt tacgcgccta
       61 aaaaagcaac gctatacctg taaaaactgt cgtacccatt ggacggctca aagttatttt
      121 gtccgactca gccattcaat cgcagatcat gttcgatata aaattgcttc tttactgact
      181 gaaaaagtat ctttgtcttt tattgcgaaa agttgtcagg tatctttgac taccgttact
      241 cgtacattga aagagtttaa aagctattta ccaaagcctt ctaagaagat tctccccaga
      301 gtattgatgg ttgatgaatt tcgttcgcac gcttccatag aagataaaat gagctttatt
      361 tgcgcagatg gtgaaacagg aaacttaatt gctgtcttgc ctacgcgtaa attacctcga
      421 ttaacaagct atttcttagg ctgcaccaat ccagaagaag tagaattctt agtcacagac
      481 atgaacgccg cttacttcca gcttaccaaa cgtgttctgc caaatgcgaa agtagtgatt
      541 gatcggtttc atattgtcaa acacatgaat caggcactca atgagttgcg tatccgtgaa
      601 atgaatgagc ttcgtaaagc aggacagaaa agtcaggcag aaaaactgaa aaagaactgg
      661 cgctttctgc taaaaaatcg tgcaagcatc aaccattatg agtacaaaac atggaaaagt
      721 ttccgagcac caaaataccc atttctcact gaagcaatga tgattgatcg attgcttgag
      781 ttttctgcgc ctctaaagga ggcgtatccc ttttttcatg aattagtcga agcctttcga
      841 gacaaagacc ctgacttatt tttctcctta ttggcagaac ttcccgaaac gttagatgac
      901 ggctttcggg agaagcttca aaaccttctg acctatgaag aaggtatcac cagcgcaatg
      961 atctatcctt attccaatgg aaaaattgaa gcgaagaata cccacataaa gacaatgaaa
     1021 cgagtatcct acggatttaa atcattcgag aacatgagaa ttagaatctt tttgatcaat
     1081 caattaatca atgtaagata acaaaaaatc tgaaccagaa tgtggttcat tctgacccgg
     1141 atttgatttt gcacaactca tcagtcctta ttgacaaaga gccaaaaatc tgagccagaa
     1201 tgaggttcat tctgacccag atttgatttt gtacaaccca tcagtcctta ttgacaaaga
     1261 gcccttcttt ttagtattat tataattctt ccatgcgttc ttccagtcgc tgtaaatcgc
     1321 ttggacttaa ttccccaatt tttttccatt tgaccttttt gttgtttttg ggtaattcgt
     1381 ttagagttcc tacgtcaatc caagattctt tttttagtcc tgcctgtttc caatccctta
     1441 tttcaacgta tttcagcttt attttatcgg atttagtatg atattttgaa gtaattgaca
     1501 gaaacgaaat tgtgtccagt ccatttttta gaatatagac aggccggcgt ttaccgccct
     1561 ctgcagctgt gaaagcaacg tagacggttg ctatatcatt agtattcatc ttcattgaac
     1621 cattcttcaa ttttttcgtc agtgtctagt ttttggacag gcaatttttc cgctaatctt
     1681 tgaatattat ggattcgttt acttttttcc gacaattcaa tagaaaatgg gatttttcct
     1741 tgctcaacaa tttgcttata aaaaacggtg acggctgttt tggggctcat gcctagttca
     1801 tcaataatgc tttctgcttc atttttcaat ttttcgtcca tagaaatttg taacttagcc
     1861 attttctcca cctctttctg acaaacacat tatatcacgg aaagagttca aatggaatta
     1921 atacaactac ttttgaactc cttgaaatgc agtgtattta tctaaatata aggaaaggat
     1981 atagtgctaa atggccttga tagatagaaa agtttcaaaa tccgccacag cgataattga
     2041 agcgaacact caacgacaca cagaacagga aaagactcct aaaagaacca aattaactag
     2101 gacgtgtctg aaaactccac aaagatgtgg tatgctctgg taaaaaacga atggcacaac
     2161 cacgaagacc gatatggttc ttggaaaact ttctccaccc tttttgtaat tggagggata
     2221 gtggtttatt gatagcgatt ttccaagttc ttcaagtgga accagacatt gagcaccacg
     2281 gctaaaaagt gaatgaagtc tttattttta atcaataatt tttaatgatt accagtaatt
     2341 ggttttttca tgcgccctat ttctatttga tgtggagtta aataatgctg ataagtaaaa
     2401 atttttccgt caattaaact gccactagtg aatcctgttc tattggtaaa aagagaatag
     2461 tctgggtatt catctacacc aaatgataaa gattcagcca taaactgttt tttaccagca
     2521 aattcagcac tgctaccttg atggatatgt cctacaaaaa tacctaaaat atcactttcg
     2581 gcaatcaatt cagcaaaacc ttccggaact tcagttacaa tagatggatc ttcccacgac
     2641 aatggatgat gaaccaataa aatcgttccc ttttcagcag gtgtacccaa ccactcttgt
     2701 aattgaatca tctgtcctat cgatattttt ccttcgtgct gggtatcttg agcagaatcc
     2761 aaggaaataa ttcgtaaccc attttgattc gtacaatgga tatactctcc cgtttcattg
     2821 ctttcaacac tcattccttc aaagaaaaca ctgcgacgat catgattccc acgacaaaaa
     2881 tagtaaggtg tggttgggaa atattgatcc aacattttcc gtaagacttg ataatcagaa
     2941 atcccccctt catgtactaa atcacctgtt aatagaataa aatcgtacac tgttaacgag
     3001 agttcttgta acaatttttc caatgccaac tgtggatttt taattgttgg aaatagttca
     3061 gcagtaaaca agttagtttg atagttttct ctaaaatgga tatcagagag atgcaaaaag
     3121 cgcatggcta ttccctcttt cttcaataaa ataatcagga tatcctaaat tttcttgcgt
     3181 ctgataatca aaaaaatgca ttctttcagg ttctatgcgg agattgactg catcaccaat
     3241 ctgccaagta ggtttttcat taaggacaat catttctata ccatcgactt taacggtgta
     3301 cgcataactt tgacctaata gctcacgata cttcacttga ccgtgtaaat cgcttgtttc
     3361 ttttgaaaag gttacgtgtt ctggtcttat gcctaaatac aatgagccac taacctgaag
     3421 aagttgtttt tgccaagctg gattcaatgt taaaaattga ctggctacct ttaatgttcc
     3481 ttgatcataa gtaactggca taatattcat gcctggcgaa ccaataaaat ttgcagtaaa
     3541 agtattttta ggtttcgtat aaacattttt cggcgtatca atcatctgaa ttctaccttc
     3601 attcaataaa acaatccggt cagctaacgt catcgcttca atttgatcat gagtgacgta
     3661 gacaaaagtt tgtttataac gttgatgaat ttttaccaat tcttttcgtg catccaatcg
     3721 taaacgcaca tccaaatttg ataacggttc atctaataaa aaataattac tccgtttaac
     3781 caccgcacga gccaatgcta cacgctgccg ttgccctcct gacaaatctt taggaagtcg
     3841 atcttggtat ggcgttaaac ctaacatctt caaagcttct tccaaacggt gctgaatgtc
     3901 agctgtctta attttattcg cccttaatgc aaaaataata ttctccttta cagtcatgtg
     3961 aggatataaa gcataatttt gaaaaaccat tgcgatctct cgttctccac aaggtagctt
     4021 atttgctagt tgtgtaccta aaaaaagctc tccactcgta attttttcca aaccagcaat
     4081 cattctcagc aaagtagatt ttcctgaacc agatggtccc agtaacacaa ttcgttctcc
     4141 agcggccaca gctaaatcaa tttcttttaa aatggtatgt tctccatatt tttttccgat
     4201 atttcttaat tcaagcgttt ctcccatgat ttgtcctcca atttatttaa tacctgcaga
     4261 aacaaaagtc ccgataatat atttttgaaa aattgcatac aagactaacg gtacaaacat
     4321 tgtaattaaa ctaatcgcca ttgctacggt aaaatttgtc ccaccttcag aactaatata
     4381 ggtctgcatc gccaacggca aagtataatt atctactgtc cgtaaaatta ataccggcca
     4441 aacatactca ttccaagagg taataaaaaa ccaaataccg gtagaagtaa tcgcatgtct
     4501 agtaagagga aaaacaattt tactcatgac ggttgtttgt ttgatgttat ccaatgccgc
     4561 cacatctaga aggctcactg gaatttcccg cattgtttgc gtcaataaaa aaatgcccat
     4621 cgcatcagca aattgtggta ttatcacacc ccaagtagta tcaattaaat taagttttgc
     4681 aatcaacaaa taattaggaa tcatcgcaac agtaaaagga atgaacatcg taccaatgat
     4741 gataaagtac cagattttct tccctttgat tcggcaataa gtaaaactat atgccgcaaa
     4801 aaaagctacg cctaacttaa caaccgttat aattgtagca ataaaaaaag tatttctcgt
     4861 aattttgata attggaagat tttcgaacaa atagcaaaaa ttttccaagg taaacggctg
     4921 tggtatcaaa ctagtaatcg tctgataggc atctttcaat ggcttaaaag aattggccat
     4981 aataaacaat aatggataga taataatgat tatgatgaac ataaataaga gatgtggcaa
     5041 ccagcgttta attttcatag taaaccccct tttccacaaa tttaaatatt acccctaaga
     5101 aaacagcaaa tattaataaa gttaaaactg aaattgctga agattctccg actttataca
     5161 tttcaaaagc cttttgataa gcataataga tcacgtttgc actaccatta ttcggtccac
     5221 cacgagtgat gatcttaatc ggagtaaaca catactgcag accttgaaca atggtcatca
     5281 ccaataaata tagtcctgtc gaactactca taggcaacac aattgtaaaa aaaatgcgcc
     5341 agatgggtac acgatccaac ttagctgctt cgatcatctc ttgatcaatt cccacaattg
     5401 ctgcaaaaat gacaatgaaa ttataaccaa aaactttcca agcagtaata cctgcaatca
     5461 cagcaattac ccaaccttgt aaattcatcc acataggcag tcttaaacca aaatttccca
     5521 acaagacagc aattggtccc gataccggat ttaatatcca tgtaaaaacc atccccgcca
     5581 ctaccaaaga aatcacagct ggaataaaaa ataatggttt atacaaattc tgcccttttt
     5641 ctataactat tcctaataaa aaggatgtaa tatatgggaa cacacagctc agcaagacaa
     5701 atagaacggt ataggacaaa gtattcttca acactttatg aaagactgga tcagtcagca
     5761 cctttacata gttggcaaag ccaacaaatt ctttagtggg ggccaccata ttccagtcaa
     5821 aaaaactcaa atagatcgta taaatcaatg gataaaaaac aaacacgccc aacaaaataa
     5881 ttgctggcgc cagatacaaa taacctacat agcgcttcat ttccgacagc ctcctcatcc
     5941 ttatgtcaaa agcagacgac cttgtttttc ctttcttaca ggcgctgtcg tctgcttgct
     6001 ttgcttattt gagtaactca ttaatttcct tttgagtagc tttcatcgtt tcattgacgg
     6061 aattacctga taaaatttta tcccgcatat ccaacaattt ttgttcagct tctaatccag
     6121 catctccagg aaatgaactc caactgacca tcttatccat ctgatcaatt gctggtatca
     6181 tcatctgatt ttcagctaag aaagctttta atccattttg ggcgtctgca acatctttac
     6241 gtggtggtaa ataaccagta ccttctgtcc atttagctaa agattcaaca ctataaagat
     6301 attttaagaa atcccaagtt gcttcctttt tcttatccgt ttgtgatgta acagctaaca
     6361 ttgcgccacc agcaggtagt ttaacttttt tcccttccca acttggtgac tcaactgctt
     6421 tagcagtaaa agtactattt ttttgaatgt tgtccctttg agcaatagtg gtatagacca
     6481 tcgcgacgtt accatcaata aagctttgca caccttgttc ccacgtagtg tgtaatgccg
     6541 tctggtcttt taccatcctt tgatataatt cataagcagc gataccatct ttagaagcaa
     6601 aggtagcttt gccatctttt attacacttg ccccattact ttctaaaatt gcttgttgtg
     6661 cccaattatc agcaggttct tgaatataca cgccatactt tccagttttt tcttttatta
     6721 tcttagaaaa agtttgaact tcttcccaag tttttggacc attttcatcc aagcccgctg
     6781 cttttaatat atcttgattt aaatataaaa ccggattact aatggagtaa ggaataccca
     6841 cttgtttacc atctttttca gacagtgcca tgatattgtc taaaaaatga tctttaaaat
     6901 aatcagcatt atccggatcc acttcttcta ctaaagattg aggattagta tatttgaaat
     6961 tttctgcaaa ataatcttta aaagcccatc ccacttgtac tacgtccggt atttctttgg
     7021 aggcaacaga agcttgtaaa ttttgcatca agcctttata catatcagga ttatattttg
     7081 ctacaacctt cactttgtct tgagattgat tgtatttatc tactaattca ttcactgttt
     7141 ttccacctag ggtatccgca ttaacatgcc aatactctat cgttacacga ccctccgatt
     7201 tttttgttgt cttggtattc ccactaccac aagctgccaa agagatgaca cttaaaacag
     7261 acacaacccc tataacaaga tttttcttta acataacatt aacttcctcc taatttcttt
     7321 atggccattt ttctaatcaa gaaatattat gaactaccaa tgtaaatttc tagttaattt
     7381 tctgtaaata attcgaaatt tgtgcatttc ggggtacagg gtcactggac atatccatca
     7441 agctaagaat aaagccccca tatggtcatg tatcccatct ccaaatctct taactcctct
     7501 gttttgatta taaaatagtt tattctgtgc tcctttcttc cataaatagg cccatgcaca
     7561 acgtgaggtt atattataaa caccacagcg cgcaaggtac tcaatcgttt ctagttcatt
     7621 gactgtccat acagaaaatg tcaatcctag ctgctgcccc acttcacggg ccaacttagt
     7681 taaccatcct tcatgaatat tgacgatttg aacgctttga taggctaatt tctgtaaggt
     7741 atcgatcaaa tctgcttcgg taatatcccc aagttttttc ccttcgaact gattttcaat
     7801 attataaaat atatgctttc cccaaatcgc agcccaagat aattgtacat agccactaag
     7861 aacaatttgt ggccagattt gataggtttg tgccagcgaa aaaacggcca tttccaatcc
     7921 ctcttctttt aaatcacagt taatggtcat ggttgccttc tcattggcca ctgcttgaaa
     7981 cacctgctca agggttaccg aatctgggac aacttcttac ctgtcatggg ctagtattag
     8041 cgtaccatct gtttgctgac gcacatctac ttcaaaacaa agctggcgat ccaaacaaaa
     8101 gcgaagaaat gcccagctat tctcctccgt attgtcacta ccagagtgtg cagtgatcat
     8161 ggtcattttg acccctcctt taattccgtg attttctccc acacgtccac tataccgatg
     8221 aagcgttaat ttcttgttaa tttcttgtaa attccctgcg ttttttcagt aaactagttg
     8281 taaatcttgc atgctccttc ctacgaactc atgaatcgtc gattttgccc ttctagctag
     8341 tttcaccaat taagtagttc tacattcccc gcttttaacc tactacacct ttttttgttt
     8401 tcagctattc cttcttcttg ctgaaagtcc ttgaggttct ttcttacagg ataccatcaa
     8461 ggtgttcaat ggcttattcc cattgaatga ccgagactta ctatcagatt tctgctcgtt
     8521 gaaccaagtc tttgatacag gatttcttcc gtcatcttct cacaaatacc accttgcgtt
     8581 tctctagcga ttggtcgatg tgcaccctcc cagtggactg tcactacccc tttttgctat
     8641 gcctagccca caaaaaaagc tgtcctcaca gacag
//
LOCUS       DACDXO010000086         8572 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E23605_2017
            SAMN12639989-rid9881463.denovo.087, whole genome shotgun sequence.
ACCESSION   DACDXO010000086 DACDXO010000000
VERSION     DACDXO010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639989
            Sequence Read Archive: SRR10023389
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741914.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:42:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,972
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 33 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 17 of 73
            Pseudo Genes (multiple problems)  :: 10 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8572
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639989-rid9881463.denovo.087"
                     /isolate="E23605_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..776
                     /gene="menA"
                     /locus_tag="IWM35_002465"
     CDS             <1..776
                     /gene="menA"
                     /locus_tag="IWM35_002465"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HAP9920288.1"
                     /translation="YMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFVIG
                     VILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVYVN
                     TFGSDVYDLTVNFSSGQFDLVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRDIE
                     NHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKHLD
                     QFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
     gene            complement(1590..2936)
                     /gene="gor"
                     /locus_tag="IWM35_002466"
     CDS             complement(1590..2936)
                     /gene="gor"
                     /locus_tag="IWM35_002466"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HAP9920289.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFRGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
     gene            complement(3070..4311)
                     /locus_tag="IWM35_002467"
     CDS             complement(3070..4311)
                     /locus_tag="IWM35_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HAP9920290.1"
                     /translation="MTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAYGLRS
                     RAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISAVIVS
                     DPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVAEIRK
                     NTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFGQERN
                     SLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNVYKAA
                     VDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVYKFVG
                     EVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRAPNPM
                     MILTMPVAQPVKPGDMIRKRK"
     gene            complement(4329..5252)
                     /locus_tag="IWM35_002468"
     CDS             complement(4329..5252)
                     /locus_tag="IWM35_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HAP9920291.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            complement(5473..7965)
                     /locus_tag="IWM35_002469"
     CDS             complement(5473..7965)
                     /locus_tag="IWM35_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HAP9920292.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(7972..8439)
                     /locus_tag="IWM35_002470"
     CDS             complement(7972..8439)
                     /locus_tag="IWM35_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HAP9920293.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
BASE COUNT         2354 a         1814 c         1422 g         2982 t
ORIGIN      
        1 attacatgga ttatgaaaag gctcattctg aagtatataa gtatgaagaa aatgtcattg
       61 gccgcagaaa tctatctcct gtattgattc gaaacatgat tttagctatg attgcatttg
      121 tttttgttat cggcgttatt ctaacgatca atacgggttg gcttttactt ttgatgggat
      181 ttatctgttg ttttattgga gtattctata cattcggacc gattccttta tctcgaatgc
      241 ctttagggga aattttcagt ggggtgacta tgggccttgg aatttttgcc atggtcgttt
      301 atgtcaatac attcggttca gatgtctatg atctgacagt caacttttca tctggacaat
      361 ttgatttggt aggaaatctt tgggctatag cagcaatcgt attagcttcc ttacccttgg
      421 tgtttacgat tgccaatatc atgttggcta ataatttgcg ggatcttgac cgtgatattg
      481 aaaatcatcg ctatacacta gttttttata tcggacgtcc tgcgggaaca gtcttattcc
      541 agctattgat gtatgcttgc tatttcgtga ttttaatggg gttgttgagc ggtatctatg
      601 aatggccgat cttggtcacc ttcttgacct tgccaattgt gaagaagcat ttggatcaat
      661 tcaagcaaac gttaccccag cctatcagtt tcagctattc catcaaaaat atggtcttat
      721 tcaatgccag ttatgtattg gggctattac taacaatcat tatgcaagcc atttaacagc
      781 atgaagaggt tgtgaaaaaa ctgccctgca tcaagtaata agaacagcca aataaaaata
      841 gctccttata tttttcgttt ggttgggctt attaccgcag atgcaagctt ttgaacaccg
      901 tttatcagga ggttgtgaaa aagtcgctta gtttcgagta ttaagaaaaa ttttgtgaaa
      961 atggctttcc acatttttgc ataaatttgg cttaataccg agaaactggc ttttgaacac
     1021 cgtttatcag aggttgtaaa atcagtgttt tgtcctgagt attagagaaa caaatggaaa
     1081 aatagttcct catattttgc cgttttttga gctaataccg aaggacagct gattgaacac
     1141 tatttgttcg gaaaaagtga ctaactatga cacaattttt ttcacagtcc cttttttcac
     1201 ttgatttgat tcgtaccagc gaacttgacg agaactttct tggtttggta actatatggt
     1261 attcttttaa catcctcgtt tgcttcttct aatttattta tacaaagtca cactttttta
     1321 aacatctgtt cttcgataca tttctatttc ttttaaacat cgataccaat attttgaact
     1381 taagagtgca taccaaatgg attcatttac tcttcgtcag ttacgaagat aatcaaaatc
     1441 tccaacttcc ctacttattt cgttttgaga attatcgaag caatcttaca taaataacta
     1501 aaaaacaaaa aaccagaaaa tcagtatact gattttctgg ttttacatgg tttgttcaat
     1561 aaaaagctgt tggaactgtt aatattaaat tatcgcatcg ttacgaattc ttccgctcct
     1621 gttggatgga tggctactgt attgtcaaag tcttctttag ttgcacccat cttgatagcc
     1681 acggcaaaac cttgcagcat ctcatcgaca ccaacaccaa tagcatgtaa gccgacgatt
     1741 ctttcttctt ttccgacaca gatcaatttc atttcgcatt tttggcgata atcatttaat
     1801 gcaaagtaca ttggggtaaa gcgtgaacga taaactttca attcattttc gccatatttt
     1861 tccaatgctt cttcttctgt caaaccaatt gttgcaattg gtggatgcgt aaagacgact
     1921 gtcggaacaa gtttatagtc aagataagaa tctttctttc cattaaataa acgttctgat
     1981 aaacgtctac cagctgcgat tgcaacagga gtcagatcta atttaccgat cacatcacca
     2041 acagcaaaaa tatgttcatc cgttgtattt tggtatttat ctactttgat aaaccctttt
     2101 ttgtccaatt ccacatttgt attttccaga cctaattttt cggtattcgg tacacgtcca
     2161 ccggcaaaaa gtacacaatc accagttaag gtttcaccat tttcaaatag caccgtatat
     2221 tcgtcattgt ttttagtaat ttctttagca atatgatgtg cgtaagtttt tattccagct
     2281 tcttggtaca tttggatcaa attgtccgaa agcattttat caaacgttct aagtggtcgt
     2341 tcttttcgat atgcccacat gacttcacta cctaaaccac gaaatacacc tgaaagttca
     2401 gccgcaatgt atccagcacc taaaacgatt gctctcttag gtaactctgt taattcaaaa
     2461 aatccatttg aatcaattgc gtattctcca ccaggaatgg tcattttact tgcacggcct
     2521 ccagtagcga tcaagatttt aggagcacga taggtttcac cgtttacttc gatcgtttga
     2581 ctatcgataa aagtagcgta accttttata gcctcgacat gattgctatc taatcctctt
     2641 tgataagcac cgtgtagaaa atcaatatat ttttctcgtc tttctactag accggcgaaa
     2701 tcaacatttt ttaaatctgc ttggataccg taacttgagg catctcgttc aattgtttcc
     2761 aaaatcgtac ttgcttgcca cataactttt ttaggtacac aaccgacgtt gacacatgtt
     2821 ccgcccaatt cattcgcttc aatcagcaaa acttttgcgc catgcatccc tgctctgttt
     2881 gctgaagcaa taccgccaga gccgccacca attacgatgt aatcatatat ttgcattttg
     2941 ttcctcctta ttgaagagat caatcaaaac ctttatttaa cgatcctttt tctacagaaa
     3001 acaagtgtaa cacttttctt gaattttgta tgcgaagatg cttaaaacag ggatatatca
     3061 gctttttcct tatttacgtt ttcggatcat atctccaggt ttcaccggct gtgcgactgg
     3121 catagttaaa atcatcattg gatttggtgc tcggtcaatg gattcgcctt cttcattatg
     3181 cataacttca acggtttgac tgaaatgact gaatcctgga ccataaaatt cgatttcatc
     3241 tcctacagaa aacaagtttc tctgacgtat cgtagcaatc tgtgttgcct catcataaga
     3301 taatacttca ccgacaaatt tatatactgg gattttacga cgttcaccga ataattgttc
     3361 gttttcagtt ggtgtattgt agtaaaaacc tgtagctaat tctcgttgtg ctactttcca
     3421 taactcgtcg atccattctt gcttgcagac atagttttca ggatcttcca tataactgtc
     3481 tacagccgct ttatagacat ttgacaccgt agatacataa tggatcgatt tcattcttcc
     3541 ttcgatcttg aagctgtcta cgccgttttc aatcaattct ggaatatgtt cgatcattga
     3601 catatccact gcactcatcg agaattcttc ttccacttcg cctgtatcag ttaaactgtt
     3661 tcgttcttga ccaaaaggca tatcgaacaa tccatacttc cagcgacaag attgagaaca
     3721 tccgccacgg tttgcatccc gcatcgacat atggttggac aatgtgcatc gaccagaata
     3781 agagatacac atggcaccat ggataaatgc ttcgatttcg gtatccgtat ttttacgaat
     3841 ctctgccact tcctccattg aaacttcacg agctaaaacg acacgttcca acccttcttc
     3901 tttccagaat tccaatgtct caaaattagt tgctgaagct tgagtggata aatggatcgg
     3961 cagacctggg gcttctgtcg cacaaatttc gatcaaagct ggatcagaaa caatcactgc
     4021 agaaataccg acatcacgca aactgcggaa aaattcccct gctccatctt catttccttc
     4081 atgggtgacc atattagcag caacgtatac cttcgcatca tgttcttttg caaaagcaac
     4141 tccttcagcc atttcttcat aagtaaaatt acctgcacgg cttcttaaac catatgcatt
     4201 cccgccaata tatactgcat ctgctccata atggatcgcg gtttttaact tttccagtgt
     4261 tcctgctggc gctaggattt ctggtcgttt caattttctt tttactgtca tttctttcat
     4321 catactcctt atttgatagc agaaggatca atataaaaga atcctgtatc taattcacgg
     4381 ttttcaggat gcaatttact gatttcgcta gtcatttttt ccgctttttc ttgatcccag
     4441 ctaccttcta aaatttgttt ttttgcttcg gcgaatagtc gagtgatgtt tacgaatttt
     4501 tcgcctggag taaataatcc gtccaatttc catgtacagt aatgatgctg ataaagttca
     4561 ttcaattcat tcgagagatt cagatcattg ctggcaaaaa tatgtgtgcc atgactatct
     4621 tcataaatag aataatgtgt ctcttctttt ttaggttccg agataaaaag acctctttca
     4681 cgatcttttt gttcgtcttg tttcgtatag ttatagtaat tctgtagcag tggacgtttg
     4741 gattgatgga tgcatgtagc accatacaca agggtttcta caggaatatc gagtttttct
     4801 tccatcgcct tcatctcttc aaaaggaact tcacgagcaa gtactgctcc aactgcgcct
     4861 tttttcgccc agaaattgat ttgacgtgcg ctcgttacta atgtttcagc atcataaata
     4921 tacggtatac gcaattcagg attttttttc atcgtataaa taatacctgt atcaccaact
     4981 gtgatcacat ctacgtttat ttcttttaaa aaagccaaat attcaggtac atttttcatt
     5041 ttttcaggat gaatgatccc atttacagca atattcgctt ttttaccata cttgtgtgct
     5101 aattctacca attgtcgctg ttcttctcgt gtaaatgaag caggcagacg caatccaaat
     5161 tgctcttcac cgaaatatag tgtatctgca cctgcagcaa gcaactgttc tgcttgttca
     5221 atagtttcta ctgtggttat gagttcgatc ataattttct cctcctacaa cttcataata
     5281 gtagcatagc tctaaaaact ttccaatact tcttctattt aaagtcggat ttttgaaaat
     5341 tatttcacaa aaaatcaaaa taaagttcga tatattctgt ccaaatataa aaagagattg
     5401 tgacaaaaat cttgtcacaa cctcttaatc aagtcttact attataaaaa ttcagctaca
     5461 caggccaagt aactataggg aacctactgt ttccttttta ggtccgcgaa cattcaatgt
     5521 gatcttacct tttgaagcac ctactgttac gtggtcacct aaacgaattt ctccgccaag
     5581 caaagcctcg ctcaatcgat cttcaatttc tttttgaagt gcgcgacgga ttggacgtgc
     5641 accatattca ggatcaaatc cagctttccc gatcacatcg attgcagatg gtgtgatttt
     5701 cacttgaata tcttgttctt tcaatcgttt gataatcgct ttgctcatga tttggacgat
     5761 tgtatggatt tcatctttac tcaatgaatg gaagacgaca gtctcatcga ttcgattcaa
     5821 gaattcaggt cggaaagctt tctttaactc ttctaagata cgtttttgca tggctttgtg
     5881 atctttagta acatcttgga cattgaatcc gacatttttt tcttctcgaa tttgtgtcgc
     5941 tccgatattt gaagtcataa tcatgattgt atttctaaag tccacttttc tacctttaga
     6001 atctgttaaa tgaccatcat ccaatacttg cagaagtaaa ttgaaaacgt ctggatgtgc
     6061 tttctccact tcatctaaga gaatcactga atatggtttt tgacgaattt tttctgtcaa
     6121 ctgtccacct tcatcatacc ctacgtatcc tggaggagac ccgattaatc ggcttgtact
     6181 gtatttttcc ataaattctg acatatcaac acggatcagg gcatcttcac ttccaaacat
     6241 gacttctgaa agtgctttgg ccaattctgt ttttccgaca ccagttggtc caaggaacat
     6301 gaatgaacca attggacgat caggatcttt caatccgctt cgtgcacggc ggatcgcacg
     6361 tgaaactgct ttgacagcct catcttgacc aacgacacgt tcatgcagaa gtccttccag
     6421 ttctaacaag cgttcgcttt cttttttctc caactgctgt agagggacac cagtccactc
     6481 cgatacaact gtagcaacat cttcttctgt tacacgatca gaataacctt gctcctgttt
     6541 tgcttcaatt gcagaaactt tttgcagctt tttacgtagt gctttttctt gtctgcgtaa
     6601 ttgagcagca ttttcaaagt cttgtttttg gatcgctgct tccttttctt gtactagttc
     6661 atcgatttct agtttgatca cagttgattt tgttaagtga tcggtttgat ccaaacggac
     6721 tttcgctgct gattcatcga tcaaatcaat tgctttatcg ggtagttgac gagaagtgat
     6781 ataacgaaca gaaagattga ctgctgctcg tacagcttca tctgtgatct ccactccatg
     6841 gtgctcttca tatcttgtac gtaatccttg aagaatcact tctgcttctt caggagttgg
     6901 ctcatctact tgaatgcgag caaaacgtcg ctctaatgca gaatcttttt cgatatattt
     6961 ttgatattca tctagggtcg ttgctccgat cgtttgtaat tctcctcgag ctaacgccgg
     7021 tttcaagata ttcgaagcat caattgctcc ttctgctcca cctgcaccaa ttaaggtatg
     7081 caattcatca atgaataaga ttacttgacc atcattatag atttcgtcga tgaccttttt
     7141 cattcgatct tcaaattcac cgcgatattt cgtaccagca accaaagatc ccatatcaag
     7201 catcatcagg cgcttttctt gcatgtcttc tggcacttca cctaaaatga ttttttgagc
     7261 taacccttca gcaatggctg ttttgcctac accaggttca cccaccagta cagggttatt
     7321 ctttgtccgt cggctaagaa tctggataag ccgtttcact tctctcgaac gaccaacaac
     7381 tgggtccagc ttgttttctt ttgctaattt cgtcaaatca cgtgctaatg aatctagcgt
     7441 aggtgttcct tgcggtgttt gccgtttatc ttgtacattt acatttctac gacgattcat
     7501 tccttttcca gcagaatcac cagctagccc cattttttgt ttcagaactt gacgggcttt
     7561 tgaaaggctg atacctaagt tgatcatgat tcgagtagcc atgatacttt catcttttaa
     7621 taagctcatc aaaatatgtt ctgttccaac tttttgagaa ccagttttct tcacttcgct
     7681 gctagctaaa gcaaaaactt gtttcattct tggtgaatat ggaagaaaga ctcctttagg
     7741 gtaagaacgg ataccaccat aaccaatcag atgttcaatt tcttcataaa tttccgtttc
     7801 attcaaatcc atttcacgta atacttttcc agcaattcca ttttgctcga ccactaaggc
     7861 tagtaaaaca tgttctgaac taactgattg atgtttcaac cgttttgctt cttcttgtgc
     7921 aatttctaat actgcttggg ctctttcagt aaaaagttct tccatctaat ctcacttccc
     7981 atcttcataa cttaaacgtt ccagaaatgc tcggagaatc cgagcacgtg tatgatcttc
     8041 atattcattg aaattcaatg tgttttttgc aattgcactc aacatcaagt ttccttcttt
     8101 ttttgtaatt atttggtctt cataaagttt ttgaataata gcaaaagcat ttttctcact
     8161 gattgtatca tcaaataact gatttatctg ctcaagtaat tgttttttat ctgaaatcct
     8221 tacttttgca attcgtatat aaccaccacc accacgtttg ctttctacta gataaccacg
     8281 ttggatggta aaacgggtat taatcacata gttgatttgt gaaggaacac agttaaagag
     8341 gtctgccatt tcaatccttc gaatctcgat catttcacta ttttccagaa ttttctttat
     8401 atatgattca attaaatccg aggtattctg atgtgccatc ttattcattc cctttgacta
     8461 atattgacct taattataac gaatttgata atagaaagaa agaagaacgc tttttgttaa
     8521 ataaatcctt tagaatcaac aaaaaaaaat acggaatata ctattccgta tc
//
LOCUS       DACDXO010000087         8494 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E23605_2017
            SAMN12639989-rid9881463.denovo.088, whole genome shotgun sequence.
ACCESSION   DACDXO010000087 DACDXO010000000
VERSION     DACDXO010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639989
            Sequence Read Archive: SRR10023389
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741914.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:42:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,972
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 33 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 17 of 73
            Pseudo Genes (multiple problems)  :: 10 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8494
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639989-rid9881463.denovo.088"
                     /isolate="E23605_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..173)
                     /locus_tag="IWM35_002471"
     CDS             complement(<1..173)
                     /locus_tag="IWM35_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009325336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9920294.1"
                     /translation="MATKIADELENHVLETGMQKITTHASITAKPFFEKRGYKVINEQ
                     TVELRGQLFTNFL"
     gene            complement(593..1714)
                     /locus_tag="IWM35_002472"
     CDS             complement(593..1714)
                     /locus_tag="IWM35_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HAP9920295.1"
                     /translation="MDKYNVKTSFQKWFSAINFSGLSENSQSLISDFDFYSKKLDFQT
                     TLKVLLHAVYEELPSYREIDRAFLDQRLCKELGIDSLCYSSLSRRAPEIKQEVLMEIF
                     TQLVARISAQQPSSKTTSLQLIDSTTIPLNKAWFPWAKFRKTKSGIKLHLNLCYLDKT
                     NQYPESFTMTNASEHDRNHLEVLVDKTQATYVVDRGYFDYKLLDKLNRDGYFFVTRTK
                     SNTKITILDQIEVADTTTRDGTIISDQQVILVGGVNHVTERFRLVTVLTKGQKILRMV
                     TNLFDVSPNEVADMYQARWQIELLFKHLKQNLTIKRLYSHSEQGAINQVILTLIATLL
                     TYVIKIELNTTATLFQLKRTFHYLMFEPAEEWFKRYRPG"
     gene            2042..3451
                     /locus_tag="IWM35_002473"
     CDS             2042..3451
                     /locus_tag="IWM35_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HAP9920296.1"
                     /translation="MDDIKTIKHLRNNRDQSIDQIRKAMNINWRTAKKYADSDQLFIT
                     DPTRKNKSMMDDGWGEIIGSWLEEDLRLRRKLRRTAKAIHEQLKPLGFPGSYRTVCVY
                     ISQWKKQRYDETNYQAVDRLDHPIGEAQVDFGTMEVMHEDHMKDIQLLIMSFPYSNAT
                     SASAMPAQNQECFLEALKQLFNQCGGVPKTIRIDNLTPAVKKTKSKFEEAQLTDEFLR
                     FTIHYGCDVQVCNPRSGHEKGHVENKVGYVRYNFFSTSPIMDSFESLNKQLEEQLKAD
                     RKRTHYSKHQLIEDLWKKENKALHSLPQEDYPIFKEVEAKLNRYHEMKLDQTVIHIPK
                     AYGYSQLTVVLTWNKFKVLTPHGEILMNEYRPYMNKKRLIPWSDIMKQWSNKLNVVSY
                     SRYWKYLPARLQNYLAIDNNHLKYRRIKQLLSLLATYDIQQINEQFYELVVQEDAPSD
                     FDVNWSNYDALTKTGGVQK"
     gene            3448..4176
                     /locus_tag="IWM35_002474"
     CDS             3448..4176
                     /locus_tag="IWM35_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAP9920297.1"
                     /translation="MIGQTLEDYCKKLRLSHIITNIPRASFEAPEQFITDLFRLELET
                     RQQIKLERMEKLAKFPVPKSLKNYEWHEDIFLPSHLDKETLVSLDFLKRQENIVCVGS
                     PGTGKTHLAIALGRKACSEGVSTKFFRVNNLVEQLENALKKQQLASFKKRFETTELII
                     LDEMGYLPLTKEGAELLFQLISDWYEQKSIIITSNLEFSQWNRVFMDSRLTAALVDRL
                     IHHAHIISFKGESFRLKNSLSKSY"
     gene            complement(4304..5215)
                     /locus_tag="IWM35_002475"
     CDS             complement(4304..5215)
                     /locus_tag="IWM35_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP9920298.1"
                     /translation="MKIKIERSNKEKIPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GIYKNFTAIDQHTTHEKVVVKEKIVNTSGVENFTVDFVKEYFSWKNNKEVIEKRMTNL
                     EQYLTEEGLALSQDMVRADIPTSSEVVSVKILDVEKHSEEFVVSFVVDQKITEGKKTQ
                     SISSAYRLMIFEDEKGNHIVTSLPTMIGKPEKAKHKVRQVKSDSKIDSKTTEEITEFL
                     ETFFRLYPAASEKELEHYVEKDAMQPINTNLKFVELVNPIYKQKGKAIQVIVGVEYLD
                     ELSKTTSVFQYRLKLYKNENWTISNCE"
     gene            complement(5232..6239)
                     /locus_tag="IWM35_002476"
     CDS             complement(5232..6239)
                     /locus_tag="IWM35_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAP9920299.1"
                     /translation="MNLKKLSIISLTFLTISFVGILMCVGLLFGEDGEGSGSSGTSPG
                     GSSVSEVVLKHRSMVEKYAKESDITEYVSILLAIIQVESGGTMEDVMQSSESAGLSPN
                     SLSTEASIKQVCLYFSSLVKCSKELGCDQDSIIQAYNYGDGYLDYVAKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENSGWRYNYGNMFYCLLVKQYLTTTQFDDKTVQGIF
                     DEAFKYEGTAYVFGGSSPGTGFDCSGLTQWCYAKVGLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLIFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTDSYWQQHIIGAGRYK
                     Q"
     gene            complement(6236..8365)
                     /locus_tag="IWM35_002477"
     CDS             complement(6236..8365)
                     /locus_tag="IWM35_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAP9920300.1"
                     /translation="MKKKLFRIVGIVFISIVTLTVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAKSIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRTVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALSLGTKIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEGRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKRAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRTFAFGGGAAVGGKLATSSTKGKGSSKINRVSNRKNHSARSINELNSDLKK
                     QEQPVGTKKESRINLVGRKVGKVLDTKEIVKDKAKQAKNQVTDAPTNLKYSLHKGMEK
                     TKKVPEDFKRGPFEEKANRGELREKQRQRRDAKMDEKRKVLNEAWNRHEKGRENVPIK
                     KEIKPQKNELPENRSLKPRVQVHSNPEIKRKLSSQEVIPNGSTQPLSKNNSFQQVKQR
                     FTFPKRTNQQVQRKKNTRPKPKPGEKR"
     gene            complement(8370..8477)
                     /locus_tag="IWM35_002478"
     CDS             complement(8370..8477)
                     /locus_tag="IWM35_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9920301.1"
                     /translation="MQDLYGRVGVVQVHPVFEELFHAFDTRPPVQERME"
BASE COUNT         2598 a         1728 c         1487 g         2681 t
ORIGIN      
        1 attaaaaagt tagtaaataa ttgccctcta agttccacgg tctgttcatt aataacttta
       61 tatcctcgtt tttcaaaaaa tggctttgcg gtaatggaag cgtgggtagt gattttttgc
      121 ataccagttt ctaaaacatg gttttccaat tcatctgcaa ttttagtcgc aatacctttt
      181 ccttgatggc tatgtttttg tcacaacttc agtattcctg taacaatacg gatttcagta
      241 tacaacaaca gcttttgaaa agatacttgc aaaaggatta agatatctat gacctgctat
      301 gctcactgat gttgaaaaga atgttttatt aggattgagg taagcaagat agtgaatatg
      361 tccggaaagc ctgtaaaata aggcttttcg gacatatgtg aagttataaa aagatagcga
      421 aaaatctatt tagtatcact aatgttgcga acatatcgtc agaatagtct gacatttatt
      481 tcttgctaaa aatttcaaaa aagtcaagtg ctgacgcagc ggatgactat tttccagaaa
      541 attttagtat gtattcaaaa atcaaacgtg acaacagcta ttaactacat cctcatccag
      601 gtctgtaacg tttgaaccac tcttctgcgg gctcaaacat taagtaatgg aaggtacgtt
      661 tcaattgaaa cagcgtcgct gtcgtattca actcaatttt tatcacataa gtgagaagcg
      721 ttgcgattaa ggtgaggatg acttgattta tcgccccttg ttcactatgt gaatatagtc
      781 gtttaatggt cagattttgt tttaagtgtt tgaacaacaa ttcaatctgc caacgggctt
      841 gatacatgtc ggccacttcg tttggcgata catcaaagag attggtcacc attcgcagga
      901 ttttttgtcc cttggtaaga accgttacca acctaaaacg ctctgtcaca tggttgacac
      961 cgccgacaag aatcacttgt tggtcactga ttatcgtccc atcacgggtt gttgtatctg
     1021 ctacttcaat ttgatccaag atggtaattt ttgtattaga ttttgtccgt gtaacgaaga
     1081 aatagccgtc acgatttagt ttgtctaaca acttgtaatc aaagtagccg cgatccacca
     1141 catacgttgc ttgcgtttta tcaacaagaa cttcaaggtg attacggtca tgctcactcg
     1201 catttgtcat cgtaaaactt tcaggatact gattggtttt atcaagataa cagagattca
     1261 aatgtagctt gatgccagac tttgttttcc gaaacttggc ccatggaaac catgctttgt
     1321 tcaacggaat agtcgttgaa tcaatcaatt gtagactggt tgtttttgag cttggctgct
     1381 gagcagaaat tctggcgact aattgcgtaa aaatttccat caatacttct tgcttgattt
     1441 ctggcgctct acgagataac gagctgtaac acagggaatc aatgccaagt tccttgcaga
     1501 ggcgctgatc taaaaaggcg cgatcaatct cgcgatagct tggcaattct tcataaaccg
     1561 catgaagtaa cactttaagc gtcgtttgga agtctagttt tttactgtag aaatcaaagt
     1621 cgctaattaa gctttgagag ttttctgata aaccagaaaa atttattgca gaaaaccatt
     1681 tttgaaatga ggtttttacg ttatacttat ccatgcgtga gtccttttta ttggtcttgg
     1741 ataagtcatc cgagaatagt ataaaggact tttttgcgtt tggatagctt tgtattaggc
     1801 ttttgatggg ttaaaaagtc tttgcaacac tagtaattta gtatttatgt ataaattgaa
     1861 agatatttta ccaccactta gtcttcttaa aagtgatcca acattttgtt tgtggcagaa
     1921 tttaactaca cactaatgca gccgaaagta cacaactatg cagcagtttg actgcgaact
     1981 ggtttccttc catcataagt ttgaatgact tagtgatgga ggtacagaaa ggttgttaac
     2041 tttggatgat attaaaacaa taaaacattt gagaaataat cgtgatcagt caattgatca
     2101 gataagaaaa gcaatgaata tcaattggcg aacagcgaaa aaatacgccg atagtgatca
     2161 actattcatt acagatccca ctcgaaaaaa caaatccatg atggatgatg gttggggaga
     2221 aatcatcggt agttggctag aagaagacct acgtttacgc agaaaacttc gtagaacggc
     2281 caaagctatc catgaacaat tgaaaccttt gggtttccct ggctcctatc gaacagtttg
     2341 tgtatacatt tctcaatgga aaaagcagcg ttacgacgaa acgaattacc aagctgtcga
     2401 tcgtcttgat catccgatcg gagaagcaca ggtcgatttt ggaacaatgg aagttatgca
     2461 tgaagatcac atgaaagata tacaacttct tatcatgtca tttccctata gtaatgcgac
     2521 gtccgcaagt gccatgccag ctcaaaacca agagtgtttc ctggaagcat taaaacaact
     2581 atttaatcaa tgcggcggcg ttccaaaaac gatcagaata gataacttaa ctccagcggt
     2641 gaaaaaaaca aaatcaaaat ttgaagaagc acaactaacc gatgaattct tacgtttcac
     2701 cattcattat ggttgtgatg ttcaagtctg taacccacgt agtgggcatg aaaaaggaca
     2761 tgtcgaaaat aaagtgggtt atgtccgata caatttcttc tcgacttcgc caatcatgga
     2821 ctcttttgag tccctaaata agcaacttga agaacaatta aaagcagatc gaaaaaggac
     2881 gcattattca aaacatcaat taatcgaaga tttgtggaag aaagaaaata aagcgttaca
     2941 ttctcttcca caagaggatt acccaatttt taaagaagtg gaagctaaac taaatcgtta
     3001 tcacgaaatg aaattggatc aaacggttat tcatattcct aaagcatatg gctattctca
     3061 attgactgtt gttttgactt ggaataaatt caaagttctt acgcctcatg gagaaatatt
     3121 aatgaacgaa tatcgcccct atatgaacaa aaaaagattg atcccatgga gtgacatcat
     3181 gaaacaatgg agtaataaat tgaatgtcgt tagttattcg aggtattgga agtacttgcc
     3241 tgcacgcctt caaaattatc tagcgattga taataatcac ctgaaataca gacggataaa
     3301 acaattacta agtttattag cgacctacga tatccaacaa attaatgaac aattttacga
     3361 attagtcgtt caagaagacg ccccctctga ctttgatgtg aattggtcaa actatgatgc
     3421 attaacaaaa acaggaggtg tgcaaaaatg attggacaga cgttagaaga ttattgtaaa
     3481 aagcttcgat taagccatat catcacgaac ataccaagag cttctttcga agctccagaa
     3541 caatttatta ccgatttatt ccgtttagaa ctggagacca gacagcagat caaattagaa
     3601 cggatggaaa aattagccaa atttcctgta ccaaaatcgc taaagaacta tgaatggcac
     3661 gaagatattt ttctaccatc tcatctagat aaggagaccc ttgtctctct tgattttcta
     3721 aaaaggcaag aaaacattgt ttgtgttggc tctcctggta cagggaaaac acatctagcg
     3781 atcgcattag ggagaaaagc ctgtagtgaa ggtgtttcaa ccaaattttt ccgagttaac
     3841 aatctcgtag agcaattaga gaatgcatta aaaaaacaac agctagccag tttcaagaag
     3901 cgattcgaaa caacagaatt aattattctt gatgaaatgg ggtacttacc attgacaaaa
     3961 gaaggagctg aacttctctt ccaactgatt tcagattggt atgagcaaaa gagcattatt
     4021 attacctcta atttagaatt cagtcaatgg aatcgagtgt ttatggattc tcgattaaca
     4081 gctgctctcg ttgatcggct aatccatcac gctcacatta tcagcttcaa aggcgaaagt
     4141 ttcagattga aaaattcatt atctaaatca tattgaagga aaaaactagt tgcagcaaaa
     4201 ctctgcactt tcggctgcat ttttttgtaa aaagctattg cacaacacaa acatttgaac
     4261 gacgtaattc caaatgttgg attgctttta gataatcttt tatttattca caattgctaa
     4321 tagtccaatt ttcatttttg tatagtttta atctatactg aaaaacactt gttgttttgg
     4381 acaactcatc aagatactca acaccaacaa tcacttgaat cgctttaccc ttttgtttgt
     4441 aaataggatt cactaattcc acaaatttca aattagtatt aattggttgc atcgcgtctt
     4501 tttcaacata atgctctagt tctttttctg aggctgctgg ataaagcctg aaaaacgtct
     4561 ctaaaaactc ggtgatttct tccgttgtct ttgaatcaat ttttgaatca ctttttactt
     4621 gtcttacttt atgttttgcc ttttctggct taccaatcat agtgggtaaa ctcgttacaa
     4681 tatgattgcc tttttcatct tcaaagatca tcagacgata tgcagaagaa attgattgtg
     4741 tctttttccc ttctgtaatc ttttgatcta ccacaaatga gacgacaaat tcttccgagt
     4801 gtttttcaac atctagaatt tttactgata caacctcgga actagtggga atatccgctc
     4861 gaaccatatc ttggctcaaa gcgagccctt cttctgtaag gtactgctca agattagtca
     4921 ttctcttttc aattacctcc ttgttattct tccatgaaaa atattctttc acaaaatcta
     4981 cagtaaaatt ttctactcca gaggtattca ctattttctc tttcacgacc actttctcat
     5041 gagttgtatg ctgatcaatg gccgtgaagt ttttgtagat tccaaaagca aggctacaag
     5101 acaataaaag ccaaagggca agcaccattt ttcgatgctt gcccacggat actactcgtt
     5161 ctttcttctt ttttggaatc ttttccttgt tacttcgctc aatttttatc ttcattttcc
     5221 attcctcttt tctattgttt ataccgacca gcaccaataa tgtgctgttg ccaatagcta
     5281 tcggtaagat tggtccaacc caccggatct cctgcatgaa acattctgtt ctctcctgca
     5341 taaatgccca catgagtcac ataagtaccg gcatcataag ttgaatgaaa gaaaatcaaa
     5401 tcgcctggtt tggcatcttt taagtcaata tgagtcatag catcatattg atcctgagcc
     5461 acccgaggga gtttcaaacc aactttggca taacaccatt gagttaaacc actacaatca
     5521 aacccagttc cgggacttga cccaccaaat acataagccg ttccttcata cttgaacgcc
     5581 tcgtcaaaaa ttccttggac tgttttatca tcaaactgtg ttgtagttag gtactgcttc
     5641 actaataagc aataaaacat attgccatag ttataccgcc acccactatt ttccttaatc
     5701 gcgatgggat tgggataatc gactttttga ccacccgatt tttcttttga aaaagattcg
     5761 gctaaagcaa aactatgctt ctttccgttt ttcgcgacat aatccaaata cccgtcacca
     5821 tagttataag cctgaatgat actatcttga tcgcatccta gttctttgga acacttaacc
     5881 aaagacgaaa aatagagaca cacttgtttg atggatgcct cggtacttaa tgaattcggt
     5941 gataaacctg ctgattcaga actttgcatg acatcttcca ttgtacctcc agattcgact
     6001 tgaataatcg ccaaaagaat cgaaacatac tctgttatat ctgattcttt ggcatacttt
     6061 tccaccattg aacggtgttt caaaactact tctgagacac tactacctcc aggagaagtt
     6121 ccagaactac cacttccctc accgtcctca ccaaacaaca aacccacaca catcaagatg
     6181 cccacaaaag agatggtcag gaaggttaac gaaataatac tcaatttttt taagttcatc
     6241 ttttctcacc tggctttggt tttgggcgag tgttcttttt tctttgtact tgttgattgg
     6301 ttcgctttgg aaaagtgaat ctctgtttca cttgttgaaa ggaattgttc ttgcttagtg
     6361 gctgagtgct tccgtttgga atgacttctt gactagaaag tttccgtttg atttcaggat
     6421 tcgaatgtac ttgtaccctt ggttttaaag acctattttc tggtagttca tttttctgtg
     6481 gttttatctc cttttttatc ggaacatttt cccttccttt ctcatgacga ttccatgctt
     6541 cgttcaatac tttgcgtttt tcatccattt ttgcgtctct tcgttgtcgt tgtttttctc
     6601 gcaactcccc tcgatttgct ttttcttcga aaggccctcg cttaaagtcc tctggtactt
     6661 ttttcgtctt ttccatgcct ttatgaaggc tgtatttcaa gttagttggt gcatcggtta
     6721 cttgattttt tgcttgtttc gctttgtcct tcacgatttc ttttgtatcg agtacttttc
     6781 caacttttct accaactaaa tttattcgtg attctttctt cgtgcccact ggttgttctt
     6841 gttttttcag atcagaattt agctcgttaa tgcttctcgc ggaatggttc tttctattgg
     6901 acactctatt gatttttgaa ctacctttcc cttttgtaga actggtagct agtttcccac
     6961 caactgctgc accgccacca aaagcaaaag ttcgacccac atttcgatta agctgtcgca
     7021 attttcgatt catcaacatt tgcggtttcc gcatgactcg ccttcccatt gactgactat
     7081 cattactttg aaggttaaac atgctcataa tgtcacctaa tttgaaatag ataccggcaa
     7141 aggtcacaat ttgtagaaaa gctaccataa agaagggata ggtggccgaa atattgaaaa
     7201 acatcgtgga gatactgaat gcagtcgtaa tgactaaggt aactcccgct cgcatcataa
     7261 tcgtattgaa caaacgaata atcgcccttt tgcccaaact ctcatacgtc ggcaacatcg
     7321 acaataagaa actaatgggc agaaacatcg caaagataat gaaaaggatt tgtgagaaca
     7381 acataatccc tgtcagaaga aacacaaaaa aggaaatccc aatattaaac aacactaaaa
     7441 agaccacggt tcctaaacgg ttcatggtct tggtaatact gagattcgca ttgtcattat
     7501 cttcaatctc tgctttgact gcttcttccc gatccttacc cttattttca tccggactca
     7561 cggataaaat cttattaacc cgaccctcgc caatttcttc tatattggaa tcatcgaatt
     7621 gtaatagtaa ccagggttgt tgaacttgaa tcgaaaataa actgtctcga atcaaatcca
     7681 cactatcttt tccctggctt tctgaattag gtacaacaat cttggttcca aggctaagag
     7741 ccgcttcact aacgtctgag ctaaagtcat tgatttttgt aatataggtg ggcgcatacg
     7801 caatgaaaga acccgagagt agaaaaatca tcacaaaatt aatgacggtt cggactgctt
     7861 tggtagtttc tcgttttaat aatccggtat acgccacata aactcctaga gctaaaatca
     7921 tcaataatag gaacccaaag tagaatcccg aagtctgtaa tccattttca gtgataccgg
     7981 ctaaagtttg gatattcttt ccaatacttt tagccgtatc cgaaatgaaa tccaacttgt
     8041 aagcttcttg aaccacatag cccgtggcgt ttgataaata cagacttact gtccaaataa
     8101 aattggtaat ggcataaaga ccatacatca cgcttttccc taagccatcc ccccaattcc
     8161 aaggtaacca atcccaagaa ctatccacaa agaaatccag ttgataattg ttcagagaat
     8221 atttggaata aaggtttccg gcttcaactg tatcatcaac cagacctgtc gcctcagcta
     8281 ccgtaccgac taaactcaat agaactgtca gagtgacgat gctaataaaa acgattccca
     8341 caatccgaaa aagtttcttt ttcatcccct cactccattc tctcttgaac cggcggacga
     8401 gtgtcaaaag catggaagag ttcttcaaag actggatgaa cttgaaccac acctactcgc
     8461 ccatacaagt cttgcatcaa gcattgaccg tttt
//
LOCUS       DACDXO010000088         8290 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E23605_2017
            SAMN12639989-rid9881463.denovo.089, whole genome shotgun sequence.
ACCESSION   DACDXO010000088 DACDXO010000000
VERSION     DACDXO010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639989
            Sequence Read Archive: SRR10023389
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8290)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8290)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741914.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:42:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,972
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 33 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 17 of 73
            Pseudo Genes (multiple problems)  :: 10 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8290
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639989-rid9881463.denovo.089"
                     /isolate="E23605_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(45..1511)
                     /locus_tag="IWM35_002479"
     CDS             complement(45..1511)
                     /locus_tag="IWM35_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HAP9920302.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
     gene            1898..2494
                     /locus_tag="IWM35_002480"
     CDS             1898..2494
                     /locus_tag="IWM35_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAP9920303.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAGEKEYMNDADRFRVFG"
     gene            complement(2552..3034)
                     /locus_tag="IWM35_002481"
     CDS             complement(2552..3034)
                     /locus_tag="IWM35_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9920304.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(3140..3337)
                     /locus_tag="IWM35_002482"
     CDS             complement(3140..3337)
                     /locus_tag="IWM35_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9920305.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
     gene            3632..4390
                     /locus_tag="IWM35_002483"
     CDS             3632..4390
                     /locus_tag="IWM35_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C39"
                     /protein_id="HAP9920306.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
     gene            4450..4878
                     /locus_tag="IWM35_002484"
     CDS             4450..4878
                     /locus_tag="IWM35_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9920307.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            4924..4996
                     /locus_tag="IWM35_002485"
     tRNA            4924..4996
                     /locus_tag="IWM35_002485"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4957..4959,aa:Gln,seq:ctg)
     gene            complement(5146..5517)
                     /locus_tag="IWM35_002486"
     CDS             complement(5146..5517)
                     /locus_tag="IWM35_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAP9920308.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
     gene            5732..5917
                     /locus_tag="IWM35_002487"
     CDS             5732..5917
                     /locus_tag="IWM35_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9920309.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            complement(6000..6188)
                     /locus_tag="IWM35_002488"
     CDS             complement(6000..6188)
                     /locus_tag="IWM35_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9920310.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(6212..6475)
                     /locus_tag="IWM35_002489"
     CDS             complement(6212..6475)
                     /locus_tag="IWM35_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9920311.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            6633..6890
                     /locus_tag="IWM35_002490"
     CDS             6633..6890
                     /locus_tag="IWM35_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HAP9920312.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLITHIIELTLSVIFLIIWIYFLYKNQN"
     gene            complement(6961..7152)
                     /locus_tag="IWM35_002491"
     CDS             complement(6961..7152)
                     /locus_tag="IWM35_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9920313.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(7293..7613)
                     /locus_tag="IWM35_002492"
     CDS             complement(7293..7613)
                     /locus_tag="IWM35_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9920314.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            complement(7636..8070)
                     /locus_tag="IWM35_002493"
     CDS             complement(7636..8070)
                     /locus_tag="IWM35_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAP9920315.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
BASE COUNT         2628 a         1635 c         1314 g         2713 t
ORIGIN      
        1 aggaactgct gcaaataagc aattcctgtt tttatgttca ttgattaatg aatagagccc
       61 attagttttt tgattggttc tttcacagta aatagcaaga taccaccgac gattgctact
      121 gcaccgataa taccgaagta agcagattct gttcccggtg tgtagaagcg tgcaatttgt
      181 gcattgattg cttgtgaaga agcatcagct aagaaccaaa tagcgattgt ttgtgattca
      241 aatgctcgtg gtgctaattt tgttgtcaca gacaaaccaa ctggtgaaag acacaattca
      301 gcaataacca cgatgaagaa acttcccaac aaccacaatg gacttacttt tccacttgta
      361 ccataaagca tacccggcaa catcaatagc aagtacgata atcctgcaaa taaaagtcca
      421 agagagaatt tcactactgt agatggttga cgttcaccaa gtttcgtcca catcgttacg
      481 aatactggtg tcagaataaa gatgaaaatt ggattcaatg attgatacca gcttgcttgg
      541 atcgttacac caaatagcgt agattgtgta cgttcactag caaataaagc taagatagat
      601 gaaccttgtt cttgtaatga ccagaaaatg attgctgcaa ggaataatgg gatataagcc
      661 catactcttg gtttttcttc agcagttaca tcttttgaag tcagcatttt gataaagtaa
      721 tacaacggta agataatccc caatacactg attgtattga caaaaaagtc aattgtcaga
      781 tgaccagtta gttgtgcccc gccgaatact gcagcgataa caacaagtgc gatgatcaag
      841 ttacggatga atcctttacg ttcttcagtt gtcaaaggat tcggcggtgt tgtacccact
      901 ccgtctaatg tcttgcgtcc ttggatgatg tattgtaata atccaaagaa catcccaata
      961 gcagctaaag agaaccctaa atgatagtta tattcttgac cgattgtacc aacgattagt
     1021 ggtgctaata atgcccctaa gttgacaccc atgtaaaaaa tcgaaaatcc tgcatctctt
     1081 cgcaagtcag tctttgaata aagatgtccg accatgccag aaacatttgg tttcaaaaag
     1141 gcggttccga taataatcaa tgccatcgaa acaaacaatg tacttacacc aaatggtaaa
     1201 gccaagacga tgtggccaag cataatgaaa actccaccga agaatacagt gcgttttgct
     1261 cctaacactc ggtcagatac ccatccgccg acgatactag acatgtagat caaagatccg
     1321 tagatcgaca tgatagctaa tgctgttgct cgtggtaatc caagtccccc gttggcaact
     1381 gtatcgtaca tataatagat aagtaatgca cgcataccgt aataactaaa tcgttcccac
     1441 atctcagtga agaacaaagt tgacaatccc ttcggctgcc cgaagaagcc cttgtcttgc
     1501 tgtttttcca taatctatac cccaattctt taaattttca gaaaatatta tacatcttta
     1561 ccgaaaaatt taactaaaaa acttatttaa gaaatataaa ctaattttct ataaaagcaa
     1621 gtaattttta atggcaatat cataaaaaaa agaagataga gatttaataa ttatatatca
     1681 gctgtttttt tcaaataagc aaaaattcct aaaacacaac aatgaaagcg ataactaaga
     1741 aaatccttac ataacgattt ttcttattaa aattgttttt tgaacttcgc ttattttttg
     1801 tgagcatatc tgtttactta ctttttgtaa ggtgctatac ttcatttcgt aatcaattaa
     1861 aaaaatgcaa aacgcaaatc aaaggagaaa ttttattatg acaactttta ctgacacttt
     1921 gaaaaaccgc cgttcaatct atcatctagg acgtaatgtt tcactttcta atgaagaatt
     1981 aacaactttg atcaaagaag caattaaaga aagcccaact gctttcaatg ctcaatcaac
     2041 acgcgcagtt atccttttcg gagatgcaca tgaaaaactt tgggaaatca ctgaagaagc
     2101 acttcgccca ttgactccag cagaagcttt cccaaataca caaaacaaat tagctggttt
     2161 caaaaatggt tatggtacag tattattctt caaagatact gacgtggtaa aaggtctaca
     2221 agaacaattt gaattatatg cagacaactt cccagattgg tcagaacaat caaacggaat
     2281 cgcaacagct aatacttggg tagcattagt tgataaagga ttgggcgcaa acttgcaaca
     2341 ttacaaccca gttattgacg aagcagttgc aaaagaatgg aatatccctt caaactggaa
     2401 actacgttcg caattagtat tcggttcacc agaaacaccg gctggggaaa aagaatacat
     2461 gaacgatgcc gatcgcttcc gcgtatttgg ataattttat ttgaaataaa aaacagaggt
     2521 tgtgacaaac atcttgtcgc aacctctgtt tttattctgg atcaatcata atatcaatct
     2581 ctgcctcatc gtttaagcgt tgggacaaaa atggaactaa ttcttctaat gatgcagaaa
     2641 atttcagcat cggtcctcgc catccgtttc ggaaaaaatt catttttacg attttagaac
     2701 cttttggctg atataataca attttattga tctcagaaaa aggaacacct tttttgtcaa
     2761 agctattcaa aacaagtcct tcttctgtga ttcctctact gtccaacaaa aaactaataa
     2821 aaatcattgc tgacaagatc ccttttgcca attcatctgc tgtaggatga ctgatgagtg
     2881 tataccaaat gactagtgac actaagaagg gtatcaccaa gtagacccat cgatttcgca
     2941 cagtccgata aataatttct tttcgaacca gccaaagaaa taaaaacatc acaacaacga
     3001 ataaaaacaa tagaaccgta aagaatgtta ccatgacaac tcgcctcgct ttttcttctc
     3061 cagaaaatag gaaaagaata ctgatttacc ttctcaaaga aattggactt gcagcacgcc
     3121 gcacgtccaa ttgttttctt tattcaaatg tatcatattt tgcaacaacc atgcttgctt
     3181 tttcatctaa taattcttgg tcctctgcag cacattgggc aactttgacc acggcgcagt
     3241 ttgtcatttt tgatacaact tcaccaatga ccgtttttgt aaaaccaatt ggtttgcatg
     3301 catattgttc tcctaaatta atcatttctt ccgtcatcaa aatcctccta aactgctgga
     3361 acttttctcc acattaccac tgctatttct ttggagattt aactcattga tttttattta
     3421 cagttaaaaa tcaaccttac aggcattata gcgtactgtt ttcgttttca caattaaaac
     3481 agctgttgta tttagaagaa tttttttcaa aaaattaaaa catattcctt aaaaactaat
     3541 tatttcctac atattaaaat ataatacttc catttccaat aaaaatacat ttataatagg
     3601 aaagataaac agctgaaaag ggagatttca aatgaagaaa aaaacgttca aaaaactatt
     3661 gtttctaggg ggcacagctg ctcttttatt aagcggtaca agttattacc tacatatcca
     3721 ttctccagaa catacaatga acactgcaca aaaaagttca acacaaaaaa cgaaaaagac
     3781 aaaagagaaa gcagaagttt tactagatgt tccattagag agccagtttg attctccttc
     3841 tttagaaaat ggctgtgaag tcacttcgct ttctatgctt ttatcttttt atggttatga
     3901 aacaactaaa aaccagttag ctgagcaatt ggattacgtc cctgttttca atgcagatgg
     3961 gactcatggc gatcctaatg aaggatttgt tggtgatatc agtggcggag actgggctat
     4021 ggtcgtctat gttcccccag ttgcctccct tgctcagcaa atcgtccaaa cggattatca
     4081 cacttttcca aaaaccgatg ctcaactcga tgatatcaaa aaagcactat ctaaaggaaa
     4141 tcctgtctgg acttctgtga ctatcaattt tgaagtccca gatgaaactg attttatgac
     4201 ttggaccaca aataatggag aagtaagagt cactcctctt gttcatgctt gtgtagtcac
     4261 tggatatgac aaagaaaata tttatgtcaa cgatccctat ggtgtaaaaa atcgagcagt
     4321 accgatcaac gatttttcgg cgattttcac tgcaatgggt ggacagatgc taactttgga
     4381 gaaaagctag taattcaaac aaaaatccct tatactcaag ataccaaacc tttgaaggga
     4441 gcgtagataa tggaaaaatt cacacataaa aaaatggatc caaatgaaat tccgattatt
     4501 tttgtccgtg actgcaaggg aaatgttcaa ggtaaagtct ctattaatga gtggaacgag
     4561 cgtcgcagac ctgctaccct caatgaactg gaaatcaaat tgtatcgtca atctcttgtt
     4621 tattacgcag atcaagagta cgagaaagcc accgatttat tgaaatttct aattgctcga
     4681 acagagtata ctcgctttga atatattgaa cgtttagcaa acatctatca tattatgaat
     4741 gaacctgtca aagaatatca gttattggac accgtcttgt cagtagcaga actgattgcc
     4801 cttcctgctg gattagaaaa gaaactagta cgccgccttt tacgagtaaa gcaacaacta
     4861 tccgatcaag aaaaataact tgcgtttttt taggaaagtg ctatgattat tttgttgaat
     4921 aaatgggatg tagccaaact ggtaaggcac ttgactctga atcaagcaat tgtaggttcg
     4981 aaccctgcca tcccaatttg atgtgcttgc tgaaaaaatg aaccccctgt aaaagaacaa
     5041 tctttttgca gggggattat tgtattattt ttactacttg tatttagttt acataattaa
     5101 attatgaata ataaaatcgg gcgtattctt aatacaaaaa ctgatttacc caataattgc
     5161 taatgctttc tccggaactt tttctctagg aacttcctca tacgtttcta tgtgtgctcc
     5221 tttattgcta attttcaaat aatgattttc ttttaacaca gaaagaccat taaattcaac
     5281 cgttcgttct ttcccattgg catcagcagc tttctgcaca taccttgctg taccatattc
     5341 atttacttcc tctggttttt gtgtctttac atagacctct cctttttcaa ctaatggatt
     5401 tagctgatca agtataccag ggatttctcc ataacttcct tcagtataat atttcagtcc
     5461 aaatagcaaa ataccactaa tcgccgccag tccaatcaaa tatttaatca ctttcatctt
     5521 tctacctcct tgttatatat tcagtatatg ggtcagttaa agaaattcca tccgtcccaa
     5581 gttggagatt tttctcctac tttagaagcg agaatcgagt gtttttttag taaaaatgat
     5641 cgttatttga tattattgac gtgtggataa atcagttttt ctcgttttgt ccatgtaaaa
     5701 aatcatgttg atagcaaagg atgtgttcaa aatgaatcaa gaagaattat ttcaaaaagt
     5761 caaggaaatg attaagaacg gcaatttcga tggcgcaaaa cgatttatcg aagaacataa
     5821 agaacaatta ggaccttata aagagaaagc ccaaaatctt ttaaaagatg taaatatcga
     5881 cagtgtcaaa aacaaattta aaaatctatt caaataagta aaactttcac ttttgtaaaa
     5941 tcaaaggagg acaatgctgc tgcatcgtcc tcctttggtt cttttctgaa tacttcatac
     6001 taaaataaac gtgtcggatc gtaatgataa gtcacattgc cgttcagtga ggcttttccc
     6061 accacatatt tacgattctc caacttcagc cacgcttctc gaaaagccat taattcttct
     6121 ctttttacat caatcttttc gatttttcca tcgatcaaat cttgaataag ttgttcatat
     6181 ggattcattt ttttcctcca tctgctgtca atcaataagt tgtaaaaata ggttcatatg
     6241 cttttttggt atctagatct tctctgactt tgactgtcgt ataaataatt ttttctactg
     6301 ggatactata ataacttcct tcaccattat tgaatcggtg aacgtctttc tttgctaaaa
     6361 taccatttat tttattcgtc agtacacgtt gttcgacact gttacttaca acatagtaaa
     6421 tcacccgaat attattgatc aaatgcaaat cgatttctaa cactacatga tccatgaaaa
     6481 tcccactcct tctttcactt tgattatagc aagtttctcc tctaatttca aaatcgatac
     6541 agattttacc ggataaatcc acaaaactgc ttgcgccatt tcgcacaaag tgttattttg
     6601 tatcatggac tttatttatc ggaggcaata gtatgatcgc gatcggtcaa tttgtttttt
     6661 atattccttt ttttattatg ataagcattc ttttctatta cataaaatgg acaaaaaaga
     6721 agttttcagt tttactggcc tctttacctg ctgtatattt tacttatcaa attttttctt
     6781 ttaggcattg ggaaacaacg tctgtattaa taacacacat catcgaattg acgctttcag
     6841 taatttttct tattatttgg atctattttt tatataagaa tcagaattga atccctcgca
     6901 tgtttaaaaa gaaaacgatt aaagatgcag caaatttctg ccgatcttta atcgtttttt
     6961 ctattctgtt ataccaaaaa aatgtttgaa tcggtgaagg atcgttcctt gttttaattc
     7021 tataaattct tgtgcgatcg ggtacttttc ttccaaattt tctttgttct tttcgatcag
     7081 gcttttcgct tcttcatatt ttttatctgc acatagtgct tttacttcct gtaatacttt
     7141 tcctgcttcc atttgcctca ccctcttgcc attttattgc tttaagtttt atttaagtta
     7201 tacgcattat tctagtttca tcccaaataa ccttttttca tttttactcc tcgaactaat
     7261 cagttttcta aaggctgatt ggttcgtctt ttttattttt cctccatttc attctgctct
     7321 tctgttaatt tatctaatgc tttgattgat gctgcggctc ttgcataatc atagctgtat
     7381 aattccataa atttatcagg cagaacaaac cacgctggat gataaccgtt agctgtgaca
     7441 gggaatcctt tatcttgttg gttatctcca tcccatctca gagcaacacc tttttttcct
     7501 ttccacttta ctaaagcgat cgagtatccc ttctttgttt gtgtgccgtc ttcaataatt
     7561 gctaaaattt ctattttttc ttttggcgca cggacttctt gaggacgaac cattttctat
     7621 tcctccctta tctcattact tcacgaccaa cacactgcaa ggggcatgtg atacgacata
     7681 agaagcggtc gatccaacta atgcttgttg tatagcacct tttcctgtcg ctcccataac
     7741 gatcaaatca atattttctt gtccagggat gacattggca atcaaatttc ttggatctcc
     7801 agtttcaact attgcgtgga tctcagcaat gccgtactct ttggcatcat tgattttttt
     7861 cagcatggct acttctgttc tttgtttttc atctgcatag atttttgaaa aggcataggc
     7921 actatgggtc agctcaactt tgttgatgat tgacacgata aataagtggg cttcattgtc
     7981 ctttgctaag tcaagggctt cagcgaacgc tttgatcgat tgtcttgatc cgtcaactgc
     8041 taccatgatg ttctggtatc ttttactcat tggaaccgcc tcctttttct tttattgtaa
     8101 catctcaagc aaaaatacaa aaaatatacg caatatgtaa ataaaatgat gaacaaaagc
     8161 aatttctttc aagttattta tacatttctt ttctcatcag aacacctgtt ctaatcccat
     8221 tatttctgca ttatgtgaaa ttcctcatca tttttcacat cgagcgaaca tcattctgtt
     8281 acaaaaaacg
//
LOCUS       DACDXO010000089         7926 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E23605_2017
            SAMN12639989-rid9881463.denovo.091, whole genome shotgun sequence.
ACCESSION   DACDXO010000089 DACDXO010000000
VERSION     DACDXO010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639989
            Sequence Read Archive: SRR10023389
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741914.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:42:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,972
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 33 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 17 of 73
            Pseudo Genes (multiple problems)  :: 10 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7926
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639989-rid9881463.denovo.091"
                     /isolate="E23605_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..199)
                     /locus_tag="IWM35_002494"
     CDS             complement(<1..199)
                     /locus_tag="IWM35_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002395661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9920316.1"
                     /translation="MKKMGKCILCEKYTELTKEHVPPKKSGNTGGKKTRTGNLDDFLK
                     SDFTKGDFPKGIKRKPQGNVYY"
     gene            complement(362..1996)
                     /locus_tag="IWM35_002495"
     CDS             complement(362..1996)
                     /locus_tag="IWM35_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP9920317.1"
                     /translation="MEMIQTKRVALYIRVSTQEQALEGHSIQAQEQNGRQFAERMGYE
                     VVEVYADEGISGKSTKNRFAYQRMMNDARNHRFDLLVIWKLTRLGRNMLDVLQTVEEL
                     LRYDVGLHSISEQFDVTTSTGKLMLQLLGSFGEFERNQIAENVQMSMQSLVKDKKRYA
                     GGRRLGYVSGIDTNGQKQLVIEPEEAKIVRLIYAKYLGGAGYRAIANFLNRQGYQTVK
                     KNAFSTTAVKDILRNKIYAGYLEYARYINWETKRRKGKNPRPILVKGTHEPIISEEDY
                     QKVQERLALEGKQPKWNHTGENVLTGLLRCPECGAPMAASNVTNTLKDGTKKRIRYYS
                     CSVFRNKGASVCHANSIRAEQAETFVADRLKEIVQLPEVLPRLVAALNEEIVRQSQPL
                     EQELVVLLERKEELKTKIEKWEAALEDSPELFPMLKDRLDELTEKRRQLHIRENEILG
                     IFQQGEPIQVKDVQRILTNLDRFLAQSEKKQIKALYRTFIEKITFDPNHKEMLEITMK
                     FTHAVVQQLNEQYQIAVSKTKDTAIFVLKTPFTLTI"
     gene            complement(2000..3373)
                     /locus_tag="IWM35_002496"
     CDS             complement(2000..3373)
                     /locus_tag="IWM35_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP9920318.1"
                     /translation="MRVACYDRTSVYEEAKISNDAQYDAVKEYVLEKYEQIPQRFSDK
                     GESGEKAERPAFRQLRAWVCASEEEGVIYVWRYDRLARQAKIALEFIELCQQHQVQVY
                     SISEPLPASFSHASTNKLFVQLLFTFAEQQRNVIIENVRAGLSYKKQQRQYLSSAVPY
                     GYRLVNGKIQQEVHEARIVRYIFQLYLTKKYGYKKLCQRLTQQKLFFRERPFQPYHIY
                     SILKNPLYYGEIKGGSLGKYLGTFEPILSKATFLQAQEIRQSRCTAKKDTYLYLLRQK
                     IRCPFCGRHLSSKYQWNTKKTKTLHYYHCTDRSCQGIFLRAERVESEVLEKIQAFFQY
                     EGVYQALIAQLTQQLAEKRKDEKRKVIQQKKQEAQWIKAFEEGNCSLEELKQQLQALQ
                     KPMSTSSFLSLQQCEAKVKEWLQLKDLSSRQLLCDYVCRIEVTKDKTVCGIYFRQLTQ
                     NILERNE"
     gene            complement(3389..3628)
                     /locus_tag="IWM35_002497"
     CDS             complement(3389..3628)
                     /locus_tag="IWM35_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9920319.1"
                     /translation="MTKNKMTLKAEVLLYIQEHFSNQAFFTKPIYLDFEIRGVSAGSI
                     GGTLQALKNEGYLENHFVQRSFNRRVVKKWYLVHS"
     gene            complement(3810..6485)
                     /locus_tag="IWM35_002498"
     CDS             complement(3810..6485)
                     /locus_tag="IWM35_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF927 domain-containing protein"
                     /protein_id="HAP9920320.1"
                     /translation="MKMNHTSIQKHLQLLNTLGISYVNLRTFSTLNKKTVSHIFSTAD
                     SQAIINYLEKQKLSEGTVNITYNPIKQEVITQKKHSVRDNDIEKIRFVFIDIDPKRKE
                     GSATDSEKKKAENVMEQVERYLKEKRIESFVKVDSGNGYHIFLPINEQPNNHETILTI
                     QNFLQLLHRRFGVEDEVDIDTSVYNPSRLCKLVGTPAVKGKSTKERPHRLSSFLSIPD
                     NIDRSNCFKILEQIVEENSMMSTSRVKSHPSFREVLPFIQADYKQWLAHYPELTYTIK
                     EGDHKGVTLLIFDECPLRKHTNNSNGSCLSVTADNKIRFHCLHASDKNKDIQDFVKKY
                     PLPKPVNPSVESLESNQEYYFDQFKLNQEGVFLVKEGQEEKVACPMFIKQIRKNIEQK
                     VITLVLAYYVDHCWDEIEIEGGCLQTNNFKQLAKHGLIIKARREAEVVDFLQIQRENA
                     PIFFEHESLGWKKENEAMIFQLQDSFGREIPSVLTKNSCYQLTTNGTEAEFDALVHKY
                     VLGTNLELAWSIGIASIVLGYLNVTKANTFLSLLINLQGKSTTGKTTTLHFIASLFGN
                     PTTVLRNMNATNKAIIKLASNNQGGVPLILDELGAATQNNFSSLIYQLASGEERLRLD
                     SNCQLMPQETFSVLTLTSSEERLENYFNEKLTGLKVRYIEFSDIKFTKDANHAEKVKS
                     LSSNTFGWGIKLFLLKLFEQGITKIEATFVEAQKKLWAVLPEHSLKQRIGNNLALIYS
                     GALLIQDILGYPLRLEHIQTVLINSYEQLIVDTSLGSEQTVFDRVKTLLVMNSHKFLT
                     NSDNKHTPSTIWGKVSIQKNGDIKVNIFPIPFEDLLRREFQVKDLTYLFRQLLDSGNM
                     CAEKGRRTKRIHMNRQVLPTYEILLPSSLKSYFRL"
     gene            complement(6668..7591)
                     /locus_tag="IWM35_002499"
     CDS             complement(6668..7591)
                     /locus_tag="IWM35_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9920321.1"
                     /translation="MATNYAKKINFDVDKLYKSLIVVPKNKSEDGNYELEVLQFKIEQ
                     KSSERFMSNQLVKIKKWLPMIDQLVSNNKEAKNLCIFVLLLHGRVINLVNRKSTTKFA
                     NSFWFLKNINKTVEKNGVYKGDFHFQNAETIEREHHYFDLLVNTVVELAKWAKSNETY
                     SQVLKRIMSQETSFIYELSKSNLKYTYNEDELEILDNPENSQWIKELKREDFIETLRI
                     LKSLFDLKLAKATIMGIERDSYYWEEIGLYAPQIEGEDKEKRIDNIINYINSLSEPLK
                     CRGLDFIGTLGWTETYYEKIYKEIIEQYFPK"
BASE COUNT         2328 a         1560 c         1198 g         2840 t
ORIGIN      
        1 tataataaac atttccttga ggtttccttt taatcccctt cggaaaatca ccttttgtaa
       61 aatcgctctt aaggaaatca tctaaattac cagttctggt ctttttacct ccagtattcc
      121 ctgatttttt aggtggtaca tgttctttag taagttcagt gtatttttca cataaaatac
      181 acttacccat ctttttcata tctatcccca ctctttttaa ttcttatata caattatatt
      241 tcaaatattc attaataaat atccctattt atgataagga cctccctaac tacttctcta
      301 acgatttcaa atcgctagag aagcgttagc gcccctctaa cgttgtttga tataacctag
      361 actagatcgt taacgtaaag ggtgttttta acacaaaaat agccgtatcc tttgttttgg
      421 atacggctat ttgatattgc tcattcaatt gttgtaccac ggcatgcgtg aatttcatcg
      481 taatttccaa catctcctta tggttaggat caaaggttat tttctctata aaggtacgat
      541 acaaggcttt gatttgcttc ttctcactct gggcaagaaa acgatccaaa ttagtcaata
      601 tccgttggac atctttgact tgaatcggct ctccttgttg aaagatccct aagatttcat
      661 tttctcgaat gtgcaactgt cgtcgttttt cagtcaattc gtctaagcgg tcttttaaca
      721 tcggaaacaa ttcaggacta tcttccaaag cagcttccca tttttcaatt ttagtcttca
      781 attcttcctt tcgttccagt aacaccacga gttcttgttc caatggttgg ctttgtcgca
      841 ctatttcttc atttaacgct gccaccaatc ggggaagaac ttctggtagc tgaacgatct
      901 cctttaatcg gtcggcgaca aatgtttccg cttgttcggc acgaatcgaa ttggcatgac
      961 acacacttgc tcctttattc cgaaagactg aacaagaata atatcgaatc cgtttctttg
     1021 tgccatcctt tagggtattt gtgacattac tcgctgccat cggtgcgcca cattctggac
     1081 aacgtaacaa gcctgtcaat acattttcac ctgtatggtt ccatttgggt tgttttcctt
     1141 ctaaagcaag gcgttcttgt actttttgat aatcttcttc cgaaataatc ggttcatggg
     1201 ttcctttcac taaaatcggt ctcggattct tccccttccg tcgtttggtt tcccaattga
     1261 tgtagcgcgc atattctaaa taccctgcat aaattttatt gcgtaagata tctttgactg
     1321 ccgtggtaga aaaagcattc tttttgactg tttgatatcc ttgacgattc aaaaaattgg
     1381 caatcgctcg ataccctgct ccgcctaaat attttgcata gatcagacga acaattttcg
     1441 cctcttctgg ttcaatgacc aattgttttt gaccattcgt gtcaatacca ctgacatagc
     1501 ctaaacgtcg gcctcctgca taacgctttt tatctttcac taagctttgc attgacattt
     1561 gaacattctc cgcaatttgg ttgcgttcaa attcaccgaa actcccaagg agttgtaaca
     1621 tcaatttacc tgtcgaagtg gtcacgtcaa attgttccga gatcgaatgt aatcctacgt
     1681 catatcgcaa taattcctcg actgtttgta agacatctaa catattgcgt ccgagacggg
     1741 tcaatttcca aatcactagt agatcaaaac gatggttccg agcatcgttc atcattcgtt
     1801 gataggcgaa ccgatttttt gtggatttcc cactgattcc ttcatcggca taaacttcta
     1861 ccacttcata gcccatccgt tcggcaaact gtcgtccatt ttgttcttgt gcttgaatac
     1921 tatgtccttc taaagcttgt tcttgtgtac tcacgcgaat atacaatgcg actcgtttgg
     1981 tttgtatcat ttccatagat cattcgttcc tttctaaaat attttgtgtc agttgtcgga
     2041 agtaaatccc acaaactgtt ttatcctttg ttacctcaat ccgacaaaca tagtcacata
     2101 ataattgtcg actagagagg tcttttagtt gaagccattc tttcactttt gcttcacact
     2161 gttggagtga aagaaacgaa gaagtagaca tcggcttttg aagagcttgc agttgttgct
     2221 tgagttcttc cagtgaacaa ttgccctctt caaatgcctt aatccattgt gcttcttgtt
     2281 tcttttgttg gattactttg cgtttttcat cttttcgctt ttcggctaat tgttgcgtta
     2341 attgggcaat caaggcttga tagacgcctt cgtattgaaa aaaagcctgt atcttttcca
     2401 atacttctga ctcaaccctt tctgcccgaa gaaaaattcc ttggcagctt cggtcggtac
     2461 aatgataata atgaagcgtc ttcgtcttct ttgtattcca ttgatacttc gaactcagat
     2521 gacgaccaca gaagggacac cgaatttttt gacgtaataa gtacagatac gtatcttttt
     2581 tagctgtaca ccttgattgg cgaatctctt gggcttgtaa aaaggttgct ttcgagagaa
     2641 tcggttcaaa ggttcctaga tacttcccca aagatcctcc tttgatttcg ccatagtaca
     2701 atggattttt taggatacta taaatatgat agggctgaaa aggacgttcg cggaagaaaa
     2761 gcttttgttg cgtcaatcgt tgacaaagct ttttgtagcc atattttttg gtcaaataaa
     2821 gttgaaaaat atagcgaaca atccttgctt cgtgtacttc ttgttggatt ttcccattca
     2881 ctaaacgata cccatatggt accgcagaag acaaatattg ccgttgttgc tttttatagg
     2941 aaagtccggc acgaacattc tcaataatga cgtttcgttg ttgttctgca aaagtgaaca
     3001 ataattgcac aaatagttta ttcgttgacg cgtgtgagaa agaagcagga agaggttcag
     3061 aaatcgaata cacttgaacc tgatgttgtt ggcacaactc aataaattct aaggctattt
     3121 tggcttgacg tgccaatcga tcataacgcc aaacatagat caccccctct tcttctgacg
     3181 cacaaaccca agctctaagc tgtcgaaaag ctggacgctc cgctttttcc cctgattctc
     3241 ctttgtctga aaatcgttgg ggaatttgtt catatttctc taaaacatac tctttcactg
     3301 catcatattg agcgtcatta ctaattttcg cttcttcata aacagacgtt cgatcataac
     3361 aagccacacg catgcttgat tcccctctct atgaatgaac taaataccat tttttcacga
     3421 ctcttctatt gaacgaacgt tgcacaaagt gattttctaa atatccttcg ttcttcaacg
     3481 cttgaagagt acccccgata gatccagcag atacccctct tatttcgaaa tccaagtaaa
     3541 ttggttttgt aaaaaaggct tgatttgaaa aatgttcttg aatatacaga agaacctctg
     3601 cttttagtgt cattttattt ttagtcatta gaattttcct ttctttcatg tttgattgaa
     3661 tgaagtaaat gaagcataga tcaagcttca tttacttccg tttgggtttg ctcgctatca
     3721 tacagataag taatcagttg ttttgctaaa tcaataagaa atatttccat aacatggtgc
     3781 ctcactttca tactttggtt tattctaaat tacaatcgaa aatacgattt gagtgaagaa
     3841 ggtaatagaa tctcgtatgt tggaagaact tgacgattca tgtgaatacg tttcgttctt
     3901 ctcccttttt ctgcacacat attacccgaa tcaagcaatt gcctaaaaag ataggttaga
     3961 tcctttactt gaaattctcg ccttaacaag tcctcaaacg ggataggaaa aatatttacc
     4021 ttaatatccc catttttttg aattgatact tttccccaaa tcgtactggg cgtatgtttg
     4081 ttgtcactgt ttgttaaaaa tttatggcta ttcatcacca acaatgtttt cactcggtca
     4141 aataccgttt gctcacttcc taaactagta tctacaatta actgttcgta cgaattaata
     4201 agcaccgtct gtatatgctc taatcgtaaa ggatagccca gtatatcttg aattaggagg
     4261 gctccactat aaatcaaagc tagattatta ccaatgcgtt gcttcaaaga atgttcaggt
     4321 aaaacagccc atagtttttt ttgagcttcg acaaaagttg cttcaatttt tgtaatccct
     4381 tgttcaaaga gtttcaaaag aaataacttt ataccccaac caaaagtatt agaagaaaga
     4441 cttttaactt tctctgcgtg attcgcatcc tttgtaaact tgatatcaga aaattcgata
     4501 tatcgaactt tcaaaccagt taatttctca ttgaaataat tttccaaacg ctcttcagaa
     4561 gaggtaaggg tcaaaacaga aaaagtctct tgtggcataa gctgacaatt agaatctaat
     4621 cttagccttt cttctcctga agctaattga tagatcaacg agctaaagtt attttgagtt
     4681 gctgctccta actcatctag tattagtgga acacctccct gattgtttga tgctagcttt
     4741 ataatggctt tattggtagc attcatattt ctaagaaccg tagttggatt tccaaataaa
     4801 gaggcaataa agtgtaacgt agtggtttta cccgttgtgc tcttgccctg taggttaatt
     4861 agtaaactca aaaaagtatt agccttcgta acgtttaaat agcctaacac aatagaagca
     4921 ataccaatcg accaagcaag ttctaagttc gttcctaaaa catacttatg aaccaatgcg
     4981 tcaaattccg cttctgtacc attagttgtt aattggtagc atgagttttt agttaataca
     5041 gaaggaattt cacgtccaaa agaatcttgt aactgaaaaa tcattgcttc attttctttt
     5101 ttccagccaa gagattcatg ttcaaaaaag attggagcat tctcccgttg aatctgtaaa
     5161 aagtcaacga cttcagcttc tcttctagct ttaatgatta agccatgttt tgctaattgt
     5221 ttaaaattat tagtttgtaa acatccacct tctatttcta tttcatccca acaatgatcg
     5281 acataatagg caagaacaag tgtaatgact ttttgttcga tatttttgcg tatttgctta
     5341 ataaacatcg gacaagcaac tttttcttct tgaccttctt tcaccaaaaa tactccttct
     5401 tgattcagtt taaactggtc aaaataatat tcttgattgg attctaatga ttctacagaa
     5461 ggatttacag gttttggcaa cggatatttt tttacaaagt cttgaatatc tttattttta
     5521 tccgaagcat gcagacaatg aaatcgaatc ttattatcag ccgtaacaga caaacaagaa
     5581 ccattagaat tattcgtgtg ttttcttaat ggacattcgt caaatataag taaggtaact
     5641 cctttatgat cgccctcttt tatggtataa gttaattcag gatagtgagc taaccattgt
     5701 ttatagtctg cttgaataaa aggtaacact tctctaaaag atggatggct ttttactcta
     5761 gaggttgaca tcatggaatt ttcttcaact atctgttcaa gaattttaaa acaattactt
     5821 ctatctatgt tatcgggaat agaaaggaaa ctagataaac gatgcggacg ctcttttgtg
     5881 gactttcctt tgaccgctgg tgtaccaact aatttgcata atcttgaagg attataaaca
     5941 gaagtatcaa tatcaacttc atcttctact ccaaatcttc ggtgaagtaa ttgaagaaaa
     6001 ttctgaatag taagaatcgt ctcgtgatta tttggttgtt catttatggg aagaaaaata
     6061 tggtagccat tcccactatc aacttttaca aatgattcta ttctcttttc tttcaaataa
     6121 cgttctactt gttccataac attttctgcc tttttctttt cgctatctgt tgcacttccc
     6181 tctttccttt tagggtcaat atcaataaat acaaaccgaa ttttttcaat atcattgtct
     6241 ctcacagaat gctttttttg tgtaatcacc tcttgtttaa taggattata agttatgttt
     6301 actgtacctt cagacaattt ctgtttttct aaatagttaa taattgcttg agaatctgca
     6361 gtagaaaaaa tatgagaaac ggttttcttg tttaatgtag aaaaagttct caaattaaca
     6421 taagaaatcc ccagtgtatt taacagttgt aaatgcttct gaattgaagt gtgattcatt
     6481 ttcattaatt accactcact ttctaaaaaa tttttatttg tatgactaca ttacctccaa
     6541 cctctcataa tttcatgatc ttcagttcta caaaaatata tcccactcaa aatcttgaca
     6601 aaagaaaaaa gttgtgattt tggaaatcac aacttttttc ttttacgata aatcaatcct
     6661 tattaaatta tttaggaaaa tattgttcaa ttatttcttt atatattttc tcatagtatg
     6721 tttctgtcca tcctaatgtt cctataaaat caagtcctct acattttaaa ggttcactta
     6781 aactattaat atagttaata atgttgtcta tccttttctc tttatcttca ccttcaatct
     6841 ggggagcata tagtccaatt tcctcccaat aatatgaatc tctttcaatt cccatgatgg
     6901 tagctttggc taatttcaaa tcaaataacg attttagaat tcttagtgtc tcaataaagt
     6961 cttctctctt caactcttta atccattgtg aattttctgg attatctaaa atttcaagtt
     7021 catcttcatt ataagtatat ttcaaatttg acttgcttaa ctcatagata aatgaagttt
     7081 cttgactcat aattctttta agtacttgac tataagtttc gtttgacttt gcccatttcg
     7141 ctaattctac cactgtattc actaataaat cgaaataatg atgttctcgt tcaatagtct
     7201 cagcattttg aaaatgaaaa tcacctttat aaactccatt cttttccact gttttattaa
     7261 tattttttaa aaaccagaat gagtttgcaa atttagtcgt actttttcga ttaactaagt
     7321 taataactct tccatgtaat aataagacaa aaatacataa atttttagct tccttattat
     7381 tagatactaa ttgatcaatc atagggagcc attttttgat tttcactaat tgattgctca
     7441 taaatctttc actggacttt tgttcaatct taaattgcaa aacttctaac tcataattcc
     7501 catcttctga tttatttttg ggaacaacta tcaaactttt ataaagttta tccacatcaa
     7561 aatttatctt ttttgcataa tttgtagcca ttaatacatc tccttattca taataaattt
     7621 catatgacgc tcctctcaaa atttaccggt agactagaaa aaagaataaa agatacgtca
     7681 attcacactt tattcttaaa aaaaaactgg tctctctacc ggtaatatcg attgagtccg
     7741 cctttatcga ctttatcctt tttttatatt aaattttttc accggtagac agggagagaa
     7801 agaataaact aaaaagccct taattcttaa tgttttcacc tccttaccgt tcctaccgct
     7861 aaaatttaat ataactaaat tctataacat tagaagaaat tttaaatacc tttgataaaa
     7921 aatatt
//
LOCUS       DACDXO010000090         7601 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E23605_2017
            SAMN12639989-rid9881463.denovo.092, whole genome shotgun sequence.
ACCESSION   DACDXO010000090 DACDXO010000000
VERSION     DACDXO010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639989
            Sequence Read Archive: SRR10023389
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741914.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:42:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,972
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 33 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 17 of 73
            Pseudo Genes (multiple problems)  :: 10 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7601
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639989-rid9881463.denovo.092"
                     /isolate="E23605_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(138..428)
                     /locus_tag="IWM35_002500"
     CDS             complement(138..428)
                     /locus_tag="IWM35_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9920322.1"
                     /translation="MGEKKIKGAGILLVMSKALFLLPCTFMFIVLCYFKRRKKWCWID
                     VLLFLPFFPLIYFLEEIWDDMACDSAFLFSHSLGLMISLTIFAIVLFFLNKK"
     gene            complement(546..806)
                     /locus_tag="IWM35_002501"
     CDS             complement(546..806)
                     /locus_tag="IWM35_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9920323.1"
                     /translation="MGVDHLIVLSKLLFWIPFIIMTSAFFYLFKRPNKYLLLSACPMI
                     YFIHKIRGFYYYDSGISLFCYEIGLLASAIFFIFVLEYFRKK"
     gene            complement(943..1356)
                     /locus_tag="IWM35_002502"
     CDS             complement(943..1356)
                     /locus_tag="IWM35_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HAP9920324.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
     gene            complement(1812..2297)
                     /locus_tag="IWM35_002503"
     CDS             complement(1812..2297)
                     /locus_tag="IWM35_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9920325.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            complement(2436..2651)
                     /locus_tag="IWM35_002504"
     CDS             complement(2436..2651)
                     /locus_tag="IWM35_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HAP9920326.1"
                     /translation="MKTLDAIALTLLIVGGLNWLLVGLFELDLVAMIAGGSTTIFAKI
                     IYIVVGICAIYCLKFFPMITRKVDERY"
     gene            2875..3675
                     /locus_tag="IWM35_002505"
     CDS             2875..3675
                     /locus_tag="IWM35_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP9920327.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIEEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            3694..5562
                     /locus_tag="IWM35_002506"
     CDS             3694..5562
                     /locus_tag="IWM35_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP9920328.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRGYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELCDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            5555..6046
                     /locus_tag="IWM35_002507"
     CDS             5555..6046
                     /locus_tag="IWM35_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HAP9920329.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            6034..6588
                     /gene="srlA"
                     /locus_tag="IWM35_002508"
     CDS             6034..6588
                     /gene="srlA"
                     /locus_tag="IWM35_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174078.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates along with their translocation across the cell
                     membrane; the IIC domain forms the PTS system
                     translocation channel and contains the specific
                     substrate-binding site; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HAP9920330.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            6606..7601
                     /locus_tag="IWM35_002509"
     CDS             6606..7601
                     /locus_tag="IWM35_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HAP9920331.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVSTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
BASE COUNT         2707 a         1212 c         1444 g         2238 t
ORIGIN      
        1 tttgttacta tgaataacat aataagaaag taaagtggat tgatttgaga tctttttata
       61 cttcaaaaaa gataacaccc aactttcaaa aatatgtttt ggaagttggg acaaacgagt
      121 taatacattt taatacatta ttttttattc aaaaaaaata gcacaatggc aaagatcgta
      181 agagagatca taagccccaa tgaatgacta aaaagaaaag cagaatcaca tgccatatca
      241 tcccatattt cttcaagaaa ataaataagc gggaaaaagg gaaggaatag tagaacatcg
      301 atccagcacc atttttttct tcttttgaaa taacataaaa caataaacat gaacgtgcag
      361 ggaagtaaaa acaaagcttt actcataact aacaaaatac cagctccttt tatttttttc
      421 tcccccacat tcaaagagta acaatggtat gtacttaata atagaaaaat ggatcaactt
      481 gtatgaataa cagctgaaaa gatctgcacc aaataaaaaa cttccagcca aaactgactg
      541 gaagtttatt tttttctgaa atattctaac acaaatatga aaaagatggc cgaagctaat
      601 aagccgattt cgtagcaaaa caatgagata ccagagtcgt aataataaaa tcctctaatc
      661 ttatggataa aatagatcat cggacaagca gataagagta agtatttgtt aggccttttg
      721 aataagtaaa agaaagcact ggtcattatg ataaatggta tccaaaacaa aagtttactt
      781 aagacaatca aatgatcaac tcccatattt tttaccaaga gagatcataa catctttatc
      841 gtaaaaacaa aatatttttt tgctcatttt gaaaatttaa acatactcgt tcttatccaa
      901 atatataaaa atcgtccctc attcatcgag aggcgataag ttctaattta actcaatttt
      961 ttttgtttct tttggctgat aagctgtttt aggtaatacc aatttcagca caccgttttt
     1021 aaaggttcca gtgatttttt cttcatcaac attaggaaga tagaatgaac gtttgtaaga
     1081 gaccgaatgt ctttctttcc gtacatagtt gccatcttct ttatcttctg tatgggactc
     1141 atgattggcg gagatcgtca aagtattatc tgaatattca actgtcgtgt cttctttgtc
     1201 acagccaggc atatcagcag ttagtttgta ttctttttca ttttcgacta aatctacttt
     1261 tggataactt gaaacaccta aaaaatcatt gaatgctgga ctaaacacgt cattgaaatc
     1321 agggaacatg tcgcgaatgc taggtacatt tgccattttt gatcatcctt tcaataagta
     1381 ttttttattt atccctattt acgtaataac tctattttga aaaaaatgca agtataatgg
     1441 ttagctttgt gaatatttca tctaatacga tacaaaggct atctttctaa tttgaaaaag
     1501 ggatcattaa aataataaga agaaaatatg tcaaaaggta aaaaagtaaa ttcttctatc
     1561 caaatataga aaaattgaat ctagaaaacc gcccgacaat aaatttcatt aactcagttg
     1621 accgaaaatg aagtagatac caaaaattta tcaagagagc aagtagagaa gtgagtatat
     1681 tcataagaga aatagctgta acgaattatt aggttaatgc aaatgcttat ttatactccg
     1741 tataactttc tcagaaaaaa tgataccaat aatgacttgc tagtactgta aatccgtata
     1801 atatcatcta atcaaaatgg tgttccattt gcatctgtca tcgtaatcga tccttcgggt
     1861 gacactcgat aaaaaccaac gcttccagat cctccttggg aacggatttg tttgctggaa
     1921 gctgtgaaca aataatctcg atcaattttt tgataaaagc taataacaat atcgtcactt
     1981 tgtaattcgg aacggtgggc aataagaaat agaagtgctt tgtcagagtt ttctaatgga
     2041 agagaaggta cagtgctttc ttgcatttca gatgttgaag aaatgctaaa cgactgaata
     2101 cttgattggc tatgttgcga atctacatta ccagtagaag tattagtaat ggaagtagta
     2161 gaagtacttt tagttgaacc aacctcagtt gttgaaatgg ctgctgaaga ttctttaaca
     2221 gaattctttg ttttatatcc acaggcagaa aaaagtatta cagttcccaa caagcctgcg
     2281 taaatccctt tatgcatgat agctcctcct aaaatagatt atctttattc agtatatcac
     2341 ttaaacaaag aaaagcagat ggtttaaaat gaaaaaacct tcccaataaa gggaaggttc
     2401 atgaagataa taactttttt aataattata gctttctaat atctttcatc aacttttcta
     2461 gtaatcattg ggaaaaattt caagcagtag atcgcacaaa tacctacgac gatataaatg
     2521 atcttcgcaa aaatagttgt tgaaccacca gcaatcatag ctactaaatc aagttcgaac
     2581 aagcctacca acagccagtt caaccctccc acaatcaata aagttaatgc aatagcatct
     2641 aaagttttca ttagaattct cctttcttaa atgataaaac aattatatat caaaaagaga
     2701 aacattgctt atataacgct caggaatttt agcaatctat gcaaaaagat gaaaattttt
     2761 tttgctcttc cattagtaca cattttttag ctgaagctgt aaatttcatg tcttaatttt
     2821 ttttagtgag cttttatact ggatttgtaa gaaaaaagaa ggagaggatc acatatgtcg
     2881 gattggttgg atattgcagg aaaagtagta attgtaactg gaggttcctc aggaattgga
     2941 agaagtatcg tcgaaaattt gttgaagcag aatgcacaag tagcgaattt tgatgtaact
     3001 gaatgccgta tacagcatga aaacctacta tctttaaaag tcgatgtgtc ttctaaaacg
     3061 gacatagaag aagggatata taaagtaatg aaacatttca agacgatcga tggtctagtc
     3121 aataatgcag gaataaatat tccttctctt ctaatagata gaaatcatcc aaaaagcaag
     3181 tatgaattat cagaacaagt ttttgataag atgatcgctg tcaatcaaaa aagtgtatat
     3241 ttgatgagcc aagcagttgg aagaatcttg gtgcaaaagg gatcaggagt gatcgtgaat
     3301 ctttcttctg aatcaggatt ggaaggatca gaaggacaga gctgttatgc tgctacaaaa
     3361 gcagcgatga acagttttac tcgttcttgg gcaaaagagt taggaaagag gaatgttcgt
     3421 gtggtaggtg ttgcaccagg gatactggaa gaaaccggtt tgagaacaca agaatatgaa
     3481 gaagctcttt cttatacgag aggtataagc gtcgaacagc tgagaaacgg ttacagtcga
     3541 acaagtacga tccctctagg aaggagtggt aagctgcaag aagtggcgga tctagtctgc
     3601 tattatcttt cagaaagatc aagttatatc actggagtca cgactaatat ttcaggaggt
     3661 aaaacaagag gataaaggat aggggggacg cacgtgacgc tagtaaacag atggtaccaa
     3721 ttattgaaat tactagtcac tcacaagaaa gtttcaatcc aggaagtgca aaaagaaatc
     3781 aaaaccagta atcaaaccat aaaaaaagat attggggagt tgaatgaaga aattgccggt
     3841 attgcaaaaa tcatccaaaa aaacaaacat tttgaacttg aaatttatga ttttgatttt
     3901 tttgatgaga tcatgcaagg aaaattgaaa acagccagtg atttcaattc ggcatccaaa
     3961 agaatcgctt atttactaaa taggctgatc gaaacagagg atttcttacg tatggatgat
     4021 ttgtctgaag aagtcggtgt cagcagagga acagtggtca aggatctaaa gacattaaaa
     4081 gagatgatca atgattttga tgtaaagatt acaggcacac caaataaagg actaagaata
     4141 ataggagatg agctagaatt acgactactt ttattttatt ttgtttctcc ctacttttca
     4201 gagacgaaaa cagaacagtt tctagaagaa aaacaagcaa tcaaacattt tcagaagaag
     4261 acgaatgcta gtaaacaaga aatcaccctt ttaacgaaag ttatcggtat ttctttgaat
     4321 agaatctcta gtaactatct gttgaagaag cccatcaatc gttacagtgg gtgttttgaa
     4381 tatatccaag aattcaatga actgatcaat cagttggaag aaatctatga actaactttg
     4441 agccaatatg aaaaagattt tatcagtttt cctttgaaca tcatcaacac aagtataacg
     4501 atgataagga aagctaactc tgatttgcgc ttatatttcg accccatgat gatgcgtatt
     4561 cgacgattag atctcattga tctggatgag tccttcttat atcaagagat gaaacagcac
     4621 ctctctttat tgataaatcg ggtaattttt cgttatcgat tgactgattt attttatggt
     4681 gagatagaaa aaaagtatcc tttttcttac caaatcgcta gcgaatgcat acaagcatta
     4741 gaagctatct tgcaacataa gatctctaaa gtagagatca gctacctaac gttgtatttg
     4801 gagttagcat ttcgatatca aaaagagcat ttttcctcta aaaaagtagc cattgttcat
     4861 acgacaggaa aaggaacagc attaatgatc caaagacaaa tcaaaaggat attggggagg
     4921 gatatacaaa taacacatgt ttcagaagaa caatatgaaa aagtagatct aaacaaatat
     4981 ttcgctgttt ttacaacgat tccgttaaaa aatgtaaatg aagagacacc ggtgatccat
     5041 ttgactaatc tttttgatga ctcttggtta agggcacaat ggcaaaaagc aaaaaaaaag
     5101 cgattagccg ggtttcattc tactgttttt ttctatcaaa tatggaaaga aaaaaagacg
     5161 tatcaagaat atttaaacaa attagtcgct cctttgatca gaggctatta tgtggatgaa
     5221 gaatttgaaa aaaggatctt gaaaagagaa tatcagcaaa gcacatttgt taacaaagaa
     5281 tttgcttttc ctcacacaac caaccagcta tctcaacaca ttgttttatc agtagggcta
     5341 ataactgatc aaggtttagc agaaacagga ggaaatggag ttattttttt atttgccata
     5401 cctgaagaaa tgacggctga agttgagaat gaattatttg agctatgcga tatcctattt
     5461 gaactcataa aaatgggaat agatgagttt attgaattag caccgacaca agaatcattt
     5521 ttaaaactaa tcaaaagtaa aggagagaga cttcatgaat gatatgtatc tattcatagt
     5581 tttcgcagct tcagcttatg tgctgcaagc agttatcagt tggaagcaac taaagcattt
     5641 caatcaaaca tatagcgaac tgcgaagaaa aggaaaagta gcgattggta aacgacctgg
     5701 gaaaatccgt tcaggcacga ttgttatgct agctatagac gatcatgatc aagttttaga
     5761 agtcagaaaa atgcaagggg ttacatcttt ggcaaaattt cattcattac ctgtctatgc
     5821 gggtgaagat atccattaca tcgatcgtta ccatccctca gttcgagcgg aagatcgttt
     5881 gacacaagca gctttagaaa atgccagaga agtatactta cgaatagagg caggaaatga
     5941 aaaaagtgaa caaccttcat tgccttttat tgctatgctc aataattttc ggatatggaa
     6001 aagtacgatc aaagaaaaaa ggagtgacaa acgatggata tcataacaaa atttgctgag
     6061 ggatttatga ggctgttcca aacaggtgca gaaacattca taagctggat gacaggaatc
     6121 gtaccagttg tcttgatgct gatggttgca atgaatacgc tgattgcttt tttaggtgaa
     6181 gaccgagtaa cgaaagtagc caaattatca gcgaaaaatc cgcttactcg ttaccttgtg
     6241 ttaccgttct tatctgcttt tatgttggga aatcctatgt catttacaat ggctcgtttc
     6301 cttccagaat actacaaacc aagctattat gctgctcaag ctcaattttg tcatacgagc
     6361 aatggtgtat tcccccatat taatcctgga gaattgtttg tatggatggg gattgctcaa
     6421 gggatcgaaa ccttagggct aaattcgatg gagctggcta tccgatactt gcttgtggga
     6481 ctgctgatga actttattgg tggttggata actgatttta ctactggatt tgtatgcaga
     6541 cagcaaggaa tcgttcttag caaaaaagta gagctttcag ttgattagag aaaggagagt
     6601 tttaaatgta tggtgatatt aagatcgtaa gaggaaacgg cgggtatgga ggtcctttga
     6661 ttattaggcc gacaaaagat aaacacaagt tcatctatgt cacaggtgga ggtgagaggc
     6721 caatgatcgt agataaaata gaagaactga caggtatgga gtctatcaat gggttcaaaa
     6781 catcaatacc tgatagtgaa atcgcgcttg caatcgttga ctgtggaggg actcttagat
     6841 gtggaattta tcctaaaaaa gggataccaa ccatcaatat cgtttccaca ggaaaaagtg
     6901 gcccattagc acaatatatc actgaagata tttatgtttc agcagtaggg ttagaacaaa
     6961 tacagccact aatggaaact gatgataaca aagaaacagt aaatactgca tctccagata
     7021 aaaagtacaa atatgatact tctaaaaaga taaccgagca aaaagcagag cagcaaaact
     7081 ggatagcaaa aattggaatg ggcgcgggaa aagtagttgc tacattcaat caatcagcaa
     7141 gagaagcagt agatacgatg atccggacag tgatcccttt tatggctttc gtttcccttt
     7201 tgattggtgt gatacaaggt tctgggatcg gtaatgtatt tgctaacatt atggcacctt
     7261 tagcaggaaa tgcagttgga ttaacaatca ttggctttat ttgttcactg ccatttttat
     7321 ctcctttgct tggtccaggg gcagttatta gccaagtgat tggaacattg atcggtgtag
     7381 agattgggaa aggaaatatt cctccccaat tagcattacc ggcactattt gctatcaata
     7441 ctcaaaatgc atgtgatttt attccagtcg gtctaggttt ggaagaagca aaagctgaga
     7501 cagtagaagt aggtgtacca tcagttcttt attcaagatt tcttaacggc atcccacgag
     7561 ttatcattgc ttgggtagca agttttggat tatatagctg a
//
LOCUS       DACDXO010000091         7159 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E23605_2017
            SAMN12639989-rid9881463.denovo.093, whole genome shotgun sequence.
ACCESSION   DACDXO010000091 DACDXO010000000
VERSION     DACDXO010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639989
            Sequence Read Archive: SRR10023389
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7159)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7159)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741914.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:42:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,972
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 33 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 17 of 73
            Pseudo Genes (multiple problems)  :: 10 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7159
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639989-rid9881463.denovo.093"
                     /isolate="E23605_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..179)
                     /locus_tag="IWM35_002510"
     CDS             complement(<1..179)
                     /locus_tag="IWM35_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HAP9920332.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAIN"
     gene            complement(192..1295)
                     /locus_tag="IWM35_002511"
     CDS             complement(192..1295)
                     /locus_tag="IWM35_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HAP9920333.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            complement(1385..2320)
                     /locus_tag="IWM35_002512"
     CDS             complement(1385..2320)
                     /locus_tag="IWM35_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HAP9920334.1"
                     /translation="MKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSESSTAQSST
                     AKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKYDNVNENG
                     ESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSSESFQNYA
                     QQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGKVTESNFD
                     YIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPANVEVVTGA
                     THSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            2622..4556
                     /locus_tag="IWM35_002513"
     CDS             2622..4556
                     /locus_tag="IWM35_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP9920335.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
     gene            5013..5429
                     /locus_tag="IWM35_002514"
     CDS             5013..5429
                     /locus_tag="IWM35_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HAP9920336.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            5443..6009
                     /locus_tag="IWM35_002515"
     CDS             5443..6009
                     /locus_tag="IWM35_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HAP9920337.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKQDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            6032..7018
                     /locus_tag="IWM35_002516"
     CDS             6032..7018
                     /locus_tag="IWM35_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HAP9920338.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
BASE COUNT         2083 a         1381 c         1467 g         2228 t
ORIGIN      
        1 ttattgatag cggtagtcgc taaatcaaaa actagcattc caataaaaaa taaaaaagtg
       61 tttagccaat gtatctcatg aaaaaaagcg atagagaata atacccctac cgcaaaagga
      121 aatacactag ccacctttgt tcgtatttca actacttcta aaaagatttt tagtgtcatg
      181 gttcgtactc cttattttag ttcagaacgg tcacccatcg tgtaatggga gtcctccgat
      241 aatttgaagg tgtctttgat tggatcggtc acatatactt tatcgtcttt tgtcacgaag
      301 atcgcttccg tcccgttatt caattcgctt tcgacgtatt ccagtccttt tttcacaccc
      361 attgaaaaga ctgctgtaga caacccgtca ccatcaattg atttatctgt tatgatcgtg
      421 acacttgcga tatcattgtc gaacggataa cccgtttttg gatcaaacaa atgatggtat
      481 tttttgccat ctacttctaa ataacgctcg taaattccag aggtaactaa tgttttgttc
      541 cgttctttaa tagaacctaa aacagatccg cgagccatgt tcggatcttg gatgccgact
      601 gtccagtctt ggttttcgcc tcttgggctg tggcctaaga catagacatt accgcccaag
      661 tcaacgatgg cagtggtcac tccttgtttt ttcaagactt tcactacttc gtccgtaatg
      721 taacctttgg cgattgcacc taaatcgatg atcattcctt tttcttttag ataaacagtt
      781 ttttctttgt catttagttc gatcttatga taatccacta atttcaatgc ctgatcgatt
      841 tcttcttggg aaggttttct ggcgtcatca aaaccgattc gccataattg tgtgattgca
      901 ccaatcgcca tatcgaatcc gccttgagaa tcttggctat attcgtaggc ccgcttgact
      961 aacgtgtaga cgtcatcaga aacttttact ggtttgatcc cagcttgctc attcacttca
     1021 tcgatctcag aacctttttg attgatcgtg attttatcac caagttcttt tactcgatcg
     1081 aaggcaggtt tcaatgctga ttctttgcct tcatcataca cacggatacg aacgtaagtc
     1141 cctaacaaga actgttcatc agaatatgga tctttcaata tcttggtagc tgctgaactt
     1201 tcagtggttg ttttatttgc tgagttgctg cagccagtta gcaccataaa agagatgccc
     1261 agtgtgatta aggaaaatag tagttttttc cgcatctttt tcctctcttc attttgaaag
     1321 gtgagacgac tgcctaatct ggctatcgtc ccaccctcca acttttatta gtatgattat
     1381 gtgattattt ttctgttacg atattatcta cttcgattgt ttgagtgtcg cctttttcag
     1441 ctgcattcac taattgttgt gcgtacatga tgaatgaatg tgagctgtgt gtagcacctg
     1501 taacaacttc tacattagca ggtgagccat cttcttcgcc cattgctttg acgaattcgt
     1561 cgttcagagt agggatgtaa gtttttggtt ctgtaccgct ttttgctttc atattttcat
     1621 tatattctgt atcatcttgt tttgatttac cgtctttgtc gatataatca aagtttgatt
     1681 cagttacttt gccgccagca actgtcattt cgaattgcac acggtagccg ttagagtagt
     1741 tcttttcttc aagcttgtaa gtgccgtctt tcaaatcagc accattgtcg atttcgattg
     1801 tatctgtgtt accagcttga gcagcttgga tcaattgttg tgcgtagttt tggaagcttt
     1861 cagatgagtg agtagctcca gttacgactt ccacaccgct tgcgctttgt gcttttaaga
     1921 attggtcatt caactcagga atgtattttt ctgggtttgt gccagatttt gcttccatgt
     1981 ttttgttgta ttctgcatct tttgtttttg attcgccgtt ttcattgacg ttatcatatt
     2041 ttgattcagt gattttgccg tcttttacaa ccatttcaaa gactgcacgg taaccatttg
     2101 aataattttt ttcttctaat ttatatgtgc cgtcttgcaa atctccgcca gctacaactt
     2161 ttgcagatga ttctgttgtg ctttttgcag tcgaagattg tgctgtagat gattcagaag
     2221 aagagtttga gcttccgttg tcatctgcgc cgcaagcgcc taacactaga gctgaaaatg
     2281 ctaatacggc aaatccagtt acaaaacgtt ttttcttcat tttaataccc caccattcca
     2341 tttctagtcc acaatttttc tttgtgattc attatacatt ctaatctttt tagcctataa
     2401 gtcaactatt tttgataaat aacccatgta atatttcaaa cgttttcttt cagcaaattt
     2461 cgtgaaaaaa cgctttattt taaaatttga aaaaaatcca aaaggagtta gacaaaagaa
     2521 cagaacatct atataatata tggagattgt gtaataagta tcaaactaaa agaattatca
     2581 tatattcatt atatagatag tctataagga gcggatgatc aatggcaaag caaaatattg
     2641 tcgttgtagg agcaggatat gccggtgttt cagctacaaa atacttggca aagaaactga
     2701 aaaaagaaga tgtagcaatt actctgatcg atcgtcattc gtaccatact atgatgaccg
     2761 agttgcatga agtagctggc ggacgtgtag aaccagaagc aatccaatac gatctgcagc
     2821 atttgtttgc tcgtcaaaag aatgtacaac ttgtcacaga cactgtaatc ggtatcgata
     2881 aagaacgaaa aatcgtcaag accaaattag gttcctatcc ttttgatcag ctgatccttg
     2941 gaatgggcgg agagccgaat gactttggta cacctggtgt caaagcacac ggattcaccc
     3001 tttggtcatt tgaagattct gtaaggattc gggaacatat tgagaagaca gtagcaaaag
     3061 ctgcattgga accagatgat gaactgcgta aagccatgct gactttcgtt gtctgtggtt
     3121 ctggatttac cggtattgaa atgattgggg aactgatcga ctggaaaaat cgcctagcca
     3181 aagattataa attagatcca aacgaaatca cactgatggt cgtagaggca atgccgacga
     3241 tcttgaatat gttggatcga aatgatgctg caaaagctga acgctatttg cgtaagcaca
     3301 acgtggaatt attattgaat gcaccaatcg tagaagtggc tgctgatcat atcaaattga
     3361 aagacggacg caacattccg actcacacac tgatttggac agctggtgtc aaaggaactt
     3421 ctgacgctgc tgactttgga ttagaatcag cacgcggaca gcggttgatc gccaatgaat
     3481 atatgcaggc aaaaggctac gaagataaaa acatctatat cattggtgat ttagtctttt
     3541 atgaagaatt cccaggaaca ccgacaccgc agatcgttca agctgctgaa caaacaggac
     3601 acacagccgc agcgaatatc gtggcttcga tcaaaggcgg agaaaagcat aaatttaaag
     3661 gaaactatca aggatttatg gtttctattg gtgccaaatg gggggtagct aacctatttg
     3721 ataagatcca tttaagcgga tttttagcta tcgtaatgaa acatatcgtg aatttgaaat
     3781 acttctttga tattcgttct ggttattata tgttccaata tttaatgcat gagtttttcc
     3841 atatcaaaga tgatcgcaac gtgactcgag gacattcttc tcgttacggc aatgtcttat
     3901 ggagtgtgcc acttcgtatt ttctatggtt tagtttggtt cgttgaagct tccaaaaaaa
     3961 tcattgggaa tggtgaagtg ttcaaaccaa gtacttggtt tggtgaagga tcatggttca
     4021 ctggaaacag tgtgttccct tggacatggg aatacaatgt aacagatgtt gccagtggtg
     4081 cttctgctgc tggtgatgca accacagctg ccagcggagc aggggcaagt gccggagaag
     4141 cagcgactca agctgcacat tttggtttga gttatgcata cggtgaagaa ccaatggctg
     4201 tgattgagaa gtcaccagac tggttcacaa agatcatgca agtgatcatg ccaaacaatg
     4261 atgtagcaat gttcttccaa aaattcatgg tattctttga aattgccttg gcattagcat
     4321 tgattgctgg attattcaca tggctatgca gtgcgacaac gattgtattg gtgatcacgt
     4381 tctgcttatc aggcatgttt tactgggtaa acatttggtt catttttgtt gctttcgcat
     4441 tgatgaacgg ttccggacgt gcgattgggt tggatcgctg ggtaattcca tggctacaac
     4501 gtacactagg taattggtgg tacggtagac caagttccca gtacggcaag aaataaatag
     4561 ataaagaacg ttatttgaat gcaagatttc aatagaaagg ggctgagaca aaccgtcaca
     4621 gcctcctttt tatgtaaaat taaaggaata agatgcccga gataagcctc catgagcaag
     4681 acacaggtgt cacaacctct gttacaacct cttgttataa acggtgttcc agaagtagct
     4741 tcttcgaaaa ataagccgaa atttcacaaa aacttgaaaa gtaattttcg taaattcctt
     4801 cttatttctt gaagttgccc acttctgtca caacctcttg ttataaacgg tgttccacct
     4861 gctgcttctc ggtaataagc ccaaagaatt agaaaacatg gaaagctgtt ttctattctt
     4921 cgttcttatt actcaaagca agacacaggt gtcacaacct cttgttttta tgctaaaatt
     4981 gggtgagtta atctggaaaa aggagaacaa aaatgagcct taaagaattt gctgaaaaaa
     5041 gtctgctcaa accttgggat gtttttatta ttttgctttt gactgttctt tcttttttac
     5101 cagtcatgat ttttggtctt cagcaaaaca ccgcaacagc agaaaaagaa gcggtactgc
     5161 gtgtggatgg cacagaaatc aaaacattcc ctttgatggc tggagaagag agcaacacat
     5221 atacttacaa agatgaacac ggagattata acttgatcga gatagatggc gatcgagtac
     5281 ggataaaaga agcagattgc gatgaccaga tctgcgtacg tcgaggctgg gcaacgaaaa
     5341 atggcgaaac catcgtctgt ttgccacata aattagtgat cgaagtccgt tcaacagatg
     5401 gaggtgacga agacagtctg atttattaga ccttcacaca ccatgagtaa attacaaaaa
     5461 atgatttata tttcgctact tgttgcacaa ggagtgatca ttggtttatt ggaaaatatg
     5521 atcccttatc ctttttcttt tgcgcctggt gcaaaactag gcttagccaa tctaatcacg
     5581 atcgttgcca tttttaccat gaaaaaacaa gacagcttct tactactttg gctacgacta
     5641 ttcctgacta ctttgctagg cggaacgatt tccacattcc tttatagtat gagtggtgct
     5701 ttgcttagtt attttggaat gttattggtc aagcaattag gaccaaaacg tgtcagtatc
     5761 atcggaatta gtgcggccgg cggtttcatg cataatgtcg gacagctttt gatggcttct
     5821 ttcattgcac aatcatggac agtcatgttg tacttgccag tcctttcagt tttagggatt
     5881 ttatcaggga ttgcaatcgg gatcgcagcg aattatcttt tacgacatgt gcagacactt
     5941 caacgattcc aaaaagatta tgagcaggga accaaacaaa aatggagccc aatgtttacc
     6001 aaaaaataat cgtcacacaa ggagtttttt tatgaagcta catccaatgt gggaaaacta
     6061 tcctgcttta agtaaagaat tgacagaaac actaaaacgg atggaagaaa ctgtccgcct
     6121 gaaaaacaaa cctgtcgaac aagccatcaa agaaacgatc catgccggcg gaaagctgtt
     6181 gcgtcctgcc tatcagctat tgttctcaca atttggacca gagcaagatc gagacaaagc
     6241 catcgctttg gcagcttcta tcgaaatgct ccatacggcc acactgatcc atgatgatat
     6301 cgttgatgaa gcggatctgc gacgtggtca gcctaatatc cgtagtcagt ttggtaatgt
     6361 cgtttctgtc tatgcaggag actatttatt tgtctgctgt tttaaactat tgtctgatta
     6421 ctccacttcc ttgaaaagtc tccaattgaa ttctcgcagt atggaaaaag tattgaatgg
     6481 cgaattaggg cagatggatg atcgctataa ttatgaactt tctgtaaaag aatatttgga
     6541 aaatatttca ggtaagacag ctgaactgtt ccaattaagt tgttctgttg gcgcttttga
     6601 aagtggaaca agcgagcgct tcgcaaaaaa agcaggagat atcggacttt ctatcgggat
     6661 ggcctttcag attatcgatg atattttaga ttatacaaaa gagagccagg aaatcggcaa
     6721 acctgtacta gaagacatgc gtcaaggggt ctattcttta ccgttgatct actctttgca
     6781 aaaaaacaaa ccaaagctac taccttattt agagaagaaa gcggcaatga cagaagaaga
     6841 tgtcgacgcc gtacgcaaaa tcgttgaaca tacaggaggc gtggaagaag ctaggaagtt
     6901 agcggcctct tacacagaaa aggctttgaa ggaaataaag aaattacctg ccacctctct
     6961 tcgaacaaaa gagaatctct tttcccttac ccaactgatt ttggatcgca aagactgaat
     7021 agggaaaggt tcgatacgta aataaatttc tcagaaggaa atctacgtgt tttttgacaa
     7081 attgaattgt agttcttgta tttatgataa aaagcaaaaa taaagagcaa cgtccgttag
     7141 cgacgttgct cttttttat
//
LOCUS       DACDXO010000092         6643 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E23605_2017
            SAMN12639989-rid9881463.denovo.095, whole genome shotgun sequence.
ACCESSION   DACDXO010000092 DACDXO010000000
VERSION     DACDXO010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639989
            Sequence Read Archive: SRR10023389
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741914.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:42:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,972
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 33 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 17 of 73
            Pseudo Genes (multiple problems)  :: 10 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6643
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639989-rid9881463.denovo.095"
                     /isolate="E23605_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(1..859)
                     /locus_tag="IWM35_002517"
                     /pseudo
     CDS             complement(1..859)
                     /locus_tag="IWM35_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            1102..1557
                     /gene="tnpA"
                     /locus_tag="IWM35_002518"
     CDS             1102..1557
                     /gene="tnpA"
                     /locus_tag="IWM35_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HAP9920339.1"
                     /translation="MSNDDKSLAYTRWNCKYHIIFTPKYQRKVIYGQLRKDLGKILRK
                     LCEMKDVEIIEAHAMLDHIHMLVRVPPKICISSFMGYLKGRSAVLIHEQHANLKYKYG
                     NKSFWSKRYYVSTVGLNQKTNEKYIREQEADDRIRYIISKREYVEPFKK"
     gene            1822..1920
                     /locus_tag="IWM35_002519"
                     /pseudo
     CDS             1822..1920
                     /locus_tag="IWM35_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(2143..2373)
                     /locus_tag="IWM35_002520"
     CDS             complement(2143..2373)
                     /locus_tag="IWM35_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="resolvase"
                     /protein_id="HAP9920340.1"
                     /translation="MDEAMDHPLLNKFIKDLILQIFSMIDEQEPTESKRHRAQGIIKI
                     AKRMFSIKGVLSYTVLIQKIPNHLVYKNNVED"
     gene            complement(2603..3421)
                     /locus_tag="IWM35_002521"
     CDS             complement(2603..3421)
                     /locus_tag="IWM35_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP9920341.1"
                     /translation="MSYIYLIKENYPFLTKSEKKVADFILNSGKTIIYSTMSDIKKNT
                     KVGDATIIRFCQKIGFSGFSDLKIEIAKEDFSQKKEHPTSVKYYDEIANSLTEALYST
                     IRLLDEEKLNEAIKMITQSKNIYIFGVGSSGNTSLDLENMFLRVGVQAKAVLDPHFQA
                     QVASLLTVNDLVIIFSLSGKTKDTYDSLKIAKKNGAKIIAITNYIHSPIGKSADLVLQ
                     TAIEEFLNGGSLAGKISQLYICDLLVHGYEQNNNIDSVELREKVLRSIIDKSIE"
     gene            3582..4271
                     /locus_tag="IWM35_002522"
     CDS             3582..4271
                     /locus_tag="IWM35_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HAP9920342.1"
                     /translation="MDKKRFLDLISKGLIVSCQALPGEPFYKEEESLMPYFALAAQRA
                     GAVGIRANSVRDIIEIRKCVSLPMIGIIKRDYPPESPFITATMREVDELVETGVEVIA
                     LDCTLRNRHDGLSINSFITKIKEKYPHQLFMADISTFEEGKNAYKAGIDFVGTTLSGY
                     TEESKKQDGPDIDLIKKLVEEGICVIAEGRIHTPEQAKEIKKIGVAGIVVGGAITRPQ
                     EIASRFVKALS"
     gene            4285..5787
                     /locus_tag="IWM35_002523"
     CDS             4285..5787
                     /locus_tag="IWM35_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010759843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9920343.1"
                     /translation="MFKKFSQLGRAFMLPIAILPVAGLLLGLGGALTNESAINAYPFL
                     DQPWLHTILSIMSYAGNAVFANLALIFAIGIAVGLANGDKGTAGLAGGVAYLVYTATI
                     SGFLELFSAKDATLDTGVVGSIVVGGTVAFLHNRYRKIELPQFLGFFGGSRFIPIVSS
                     LAAIIIGSFFYLIWPPIQNGLVVAGEQIAQMGSLGTFLYGFLLRLTGAVGLHHTIYPL
                     FWYTSLGGTETVAGSTIAGAQNIFFAQLADPNHTGLFTYGTRFFAGRFATMMFGLPAA
                     CYAMYRAIPKKNRKKNGGLYFSGALTSFLTGITEPVEYMFLFVAPWLYVIHAFLDGLS
                     FYFADILNIRVGNSFSGGLIDFLLFGVLQGNDKTNWLKLIPFGIAWAFIYFFVFTFFI
                     KKFKVAIPGMEEESEVHSEKVINKNTSLHEESLIIIEALGGKENIESVEACATRLRVA
                     VTNGEIIQKQTIKDTGATAVFEVKGGIQAVFGGKADLLSQEINQILGKDN"
     gene            5800..6276
                     /locus_tag="IWM35_002524"
     CDS             5800..6276
                     /locus_tag="IWM35_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAP9920344.1"
                     /translation="MFEIFKKKKQETFYAPCSGKIISITEVNDPVFSQKLLGDGFAVI
                     PDGSKIYAPLAGRVTNIFPTKHAINIKSNSGIDYLIHIGIDTVELQGEPFSICVQEND
                     EVTPETSLVDVDFDKIIKSGKDTSVIVVFPEKNQFNDLIIDNGRIAHGNSCGKITF"
     gene            complement(6373..6483)
                     /locus_tag="IWM35_002525"
                     /pseudo
     CDS             complement(6373..6483)
                     /locus_tag="IWM35_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         2172 a         1032 c         1204 g         2235 t
ORIGIN      
        1 acttgtcctc gataagaatt gtagcgccgt gatttacgtg tgtatgtcgt tacttttatt
       61 cctagcttct tgatcaggcg ctgaactttc tttttattga tagagaaacc gcggtttctt
      121 agttcattgg tcatccgtaa acagccataa tccttgtgct tctcccgaac tttaagcatt
      181 tccgcttcaa tctcttgatc tggattttta cggtccaatc gtttttgcca gtacataaag
      241 attgccttag ggaactttaa agtttctaga agctcaacta atttaaacgg tcctcggagg
      301 cttgcgatga ttcgtgcgat tgcttcattc gtcgttgttg ggcttcctgt tttcgcagct
      361 tcctcaattc ttttaaaaag gcattttcaa tttgaaggga acggactcgt ttctccaact
      421 cctcgatgcg ttcacgttct ttagacgttg ctttagttgt agattttttc ttatttttgg
      481 gttcattttt cttagacaag ataggtggac atcctttcgt ttttgagagt ccatcgatcc
      541 caccctcacg gaattgacgc atccaactag cgatcaaatt agggttgttc atgttaagtg
      601 tgttggcaac ttcacgatag gacagctcac ttgttagata taactctatc gcatccagct
      661 taaattgaac agaatatttt ttcttttgtc gctttcttac tagaccctct tcaccaaatt
      721 cttgataggc attgatccag cgcttcactt gtgatggagc cttaatggaa tatttctttg
      781 ccagataatc acttccgcct ttaccatcca agtaatcatg aacgatcttt aatttaagtt
      841 caaaagtata cttagccata aaaaataccg acctccaaaa gttagatttt ttggtctaac
      901 ttttgggggt cggtacaaaa cttaaaagtt ttagaccctt ttattttctt aatgacttca
      961 gtcagttttg tgcgtaaagc gtgcgaaaca agaaaaccgc gcgtttctac gcgtggaaga
     1021 agtatcttta gcgataaaaa ctcctttcga tatactagtg agtggctacc aaccgctcat
     1081 cagatcgaaa ggagtttttt catgtcaaat gacgataaaa gtttagcata tactcgttgg
     1141 aattgtaagt atcatattat ttttacacca aaatatcaac gaaaagtaat ctatgggcaa
     1201 ttacgaaaag atcttggaaa aatacttagg aagttgtgcg aaatgaaaga tgtggaaatc
     1261 atagaagcac atgcaatgct agatcatatt cacatgcttg ttcgagtacc accaaaaata
     1321 tgtatatcca gttttatggg gtatctgaaa ggtcgtagtg cggtactaat ccacgagcag
     1381 cacgcaaatc tcaaatataa atacggaaac aaatcctttt ggtcgaagag atactatgtg
     1441 agtacagtcg gattgaatca aaagacaaat gaaaaataca tccgtgaaca agaagctgat
     1501 gatcgcattc gatatattat aagtaaaaga gagtacgtag aaccatttaa aaaataaaga
     1561 gaagtaagtg agtgaagatg agcagttggc ggtcttttaa aaatcccctt ttaaggggca
     1621 gcgagtgtcg accccttcta gggggattaa aaatacaccc gtcctcacgg gtgtatgggt
     1681 tactgataga cttgatttta actggtgtca gaaacttatg tccagaaagg gtgatgaaat
     1741 tttggcaatt tacggctatt caagaattag cacaagcaac caagattaca aaacacaaat
     1801 tcaaacatta gaaaatgctg gcgcagaaat aatcttccta gaaaatcact ggattatcga
     1861 acacctttgg aagtattttt gagttacgta agtattgatg atctgtctaa cttaatttga
     1921 caattaagag tatatttact tcagatggta aggcaacaaa ataaaataca ataggcagat
     1981 gaactaataa ataaaatctg tattaaatac agataaatta atataaaaat caaaaggatc
     2041 ataaactttt aacaagtgat gaaatgggtg gaaattgatt cttatagaca gtttgtctca
     2101 taacattgaa gtattttgca attttagcat tgaaacactt ttttaatctt caacgttgtt
     2161 cttataaaca agatgattgg gaatcttttg aattaacact gtatagctta gaacaccctt
     2221 tatagaaaac attcgcttag caatcttaat aattccttgt gcccgatgtc gtttagattc
     2281 agtaggttcc tgctcatcga tcatggaaaa aatttgaagg atcagatcct taataaattt
     2341 atttaagaga ggatgatcca ttgcttcatc catactcgat aaactagtga taatgagcta
     2401 cacgtttttc tttcaagttg atcaatagct tccacgataa aaatatcttt agataaaatc
     2461 aagaaccgta ggaactacag ggatatttgg tcaataagag ccaccgctat tagtaaaaaa
     2521 taagctatca agtatgctct catcccaaga attgcctact tcaatcgtta aaaaccgtta
     2581 ggtttagcta agtgggggta gttcactcaa tacttttatc aataatagag cgcaaaactt
     2641 tttctcttaa ttcaactgaa tcaatattat tgttttgttc ataaccatga actaatagat
     2701 cacaaatata aagttgtgat atttttccag ctaaagatcc accattaaga aattcttcaa
     2761 tggctgtttg taaaactaag tctgcagact ttcctatagg tgaatgaata tagttagtta
     2821 tagcaataat ttttgcgcca ttttttttag ctattttcaa tgaatcataa gtatctttcg
     2881 ttttcccaga taaagaaaaa atgattacta aatcattaac tgttaataaa gaagcgactt
     2941 gggcttgaaa atgagggtct agtacagctt ttgcttgaac tcctacgcga agaaacatat
     3001 tttctaaatc aagactagta tttccactag atcccactcc aaatatatat atgttttttg
     3061 attgagtgat cattttaatg gcttcattca atttttcttc gtctaaaagc ctaatagtgg
     3121 aatagagagc ttctgttaaa ctattagcaa tttcatcata gtactttaca ctagtcggat
     3181 gttctttttt ttgactaaag tcttcctttg caatttcaat ttttaaatca gaaaaaccag
     3241 aaaaaccaat tttttgacaa aagcgaataa tagtagcatc gccaactttt gtgtttttct
     3301 taatatcact catcgtacta taaataatag ttttcccaga atttaaaata aaatcagcga
     3361 cttttttttc agacttcgtt aaaaatggat aattttcttt aattaaatag atataactca
     3421 tacaaactcc tctacaattt caacttttct ccattatact ccaaaataat aaaaaaaact
     3481 cctaaatact ccatttttat ctttactttt atgaaagcgg atgctatatt gtttttggag
     3541 ttgttatcca aaaataataa aaaattggag tgtttaggat aatggataaa aagaggtttt
     3601 tagatcttat ttcaaaaggt ttgattgttt cctgtcaagc attacctggt gaaccatttt
     3661 acaaagagga agaaagttta atgccttatt ttgctttggc tgctcaaagg gctggagcag
     3721 ttggtatacg tgcaaattct gttcgagata ttattgaaat tagaaagtgt gtatcattac
     3781 caatgattgg aattattaaa agagattatc caccagaatc tccattcatt actgccacta
     3841 tgagagaagt agatgaatta gtagaaaccg gtgttgaggt aatcgctctt gattgcactc
     3901 ttagaaatcg acatgatgga ctatctataa attcttttat tactaaaata aaagaaaaat
     3961 atcctcatca gttatttatg gcagatattt ctacatttga agaaggcaaa aatgcatata
     4021 aagcaggaat agattttgta ggaactactt taagtggata tactgaggaa agtaaaaaac
     4081 aagatgggcc agatatagat ttgattaaaa aattggtaga agaagggatt tgtgtaattg
     4141 ctgagggaag gattcatact ccagaacaag caaaagagat aaaaaaaata ggagttgcag
     4201 gtattgttgt gggtggtgct attactcgac cacaggaaat tgcatcaaga tttgttaaag
     4261 cattaagtta gggggagaaa taaaatgttt aaaaaatttt cacaattagg acgcgctttc
     4321 atgttaccaa ttgctatatt accagtagcc ggattactat tgggactggg aggagcgtta
     4381 acaaatgaga gtgctataaa tgcttatcca tttttagatc aaccgtggtt gcatacaatt
     4441 ttaagtatta tgagttatgc tggaaatgca gtttttgcaa acctagcact aatttttgca
     4501 attggtattg ctgtaggact tgcaaatgga gataaaggaa cagctggttt agctggtgga
     4561 gtagcttatc tagtatatac tgcaactatt agtgggtttc ttgaactttt ttcagcaaaa
     4621 gatgcaacac tagatacagg tgtagtggga tcaattgttg ttggtggtac tgttgcattt
     4681 ttacacaatc gttatagaaa aattgaatta ccacaattct taggtttttt tggaggatca
     4741 agatttattc cgattgtttc tagtttagct gcaatcatta ttggttcttt cttttattta
     4801 atttggccac caattcaaaa tggtttagta gttgctggtg aacaaattgc ccaaatgggt
     4861 agtttaggaa cttttttata tggtttttta ttgcgtttaa caggtgctgt tggactacac
     4921 cacaccattt accctttgtt ctggtataca tctttaggtg gtaccgaaac tgtagctgga
     4981 tcaacaattg ctggagccca aaatattttc tttgcccaac tagctgatcc aaaccacact
     5041 ggtctgttta cttatggtac tagatttttt gcaggaagat ttgcaacaat gatgtttgga
     5101 ttaccagcag catgttatgc aatgtatcgt gcaattccta agaaaaatcg taaaaagaat
     5161 ggtgggcttt attttagcgg ggcccttaca tcatttttaa ctggaattac tgaaccagtt
     5221 gaatatatgt ttttatttgt tgctccttgg ctttatgtga ttcatgcatt cttagatgga
     5281 ttatcttttt attttgctga tatcttaaat attcgtgtag ggaattcctt ttcaggtggt
     5341 ttgatcgact ttttattatt cggtgtactt caagggaatg acaaaactaa ttggttaaaa
     5401 ttaattcctt ttggtattgc atgggcattt atatatttct ttgtttttac attctttatc
     5461 aaaaaattta aggtagctat tcctggaatg gaagaagaat cagaagttca ttctgaaaaa
     5521 gtaataaaca aaaatacatc attacatgaa gagtctctta tcattattga agctcttggt
     5581 ggaaaagaaa atattgagtc tgttgaagct tgtgcaactc gtttgcgtgt tgctgttacg
     5641 aatggggaga ttattcaaaa acaaacaata aaagacactg gtgcaactgc tgtcttcgaa
     5701 gtgaagggtg gcatccaagc tgtttttgga gggaaagctg acttattaag tcaagaaatc
     5761 aatcaaatat taggaaaaga taattaggag gtttttaata tgtttgaaat tttcaaaaag
     5821 aaaaaacaag aaacttttta tgcaccttgt tccggaaaaa tcatttccat cacagaagta
     5881 aatgatcctg tcttttcaca aaaattacta ggtgatggtt ttgccgtcat acctgatggt
     5941 tcaaaaattt atgctccatt ggctggtcga gtaacaaata tatttcctac taaacatgct
     6001 attaatataa aaagcaactc aggcattgat tatttgattc atattggaat tgatacagtt
     6061 gaattacaag gagagccttt ttcgatttgt gttcaagaaa atgatgaggt tactcctgaa
     6121 acctcgttgg ttgatgtgga ttttgacaaa ataataaaat ctggtaaaga tacttctgtt
     6181 atagttgttt ttccggagaa aaatcagttc aatgatttaa taatagataa tggtcgaatt
     6241 gctcatggga attcatgtgg aaaaatcact ttttaaaaaa gtgtatataa aaagctataa
     6301 aataagctaa ataggatatt tgtcaaatga agttggtgaa cagaaagaac tgttttcttt
     6361 ctgttcattt ttttatttta ttagtaatga ttccccatgc caaatcatga tccgtaattt
     6421 aaagttataa aagcttcgaa atccattggc atttcgttgg aataccttga tatgattatt
     6481 caaatataaa atatagagag aagagagaga gggaagatat ttataaaggg caaagttatg
     6541 tttactcaac ttaggaaata gaaatagatc aatttatatt ctcttgtcaa tcgaatatga
     6601 accttagcac tatataccaa atatagtaaa atgctgaaag atg
//
LOCUS       DACDXO010000093         6570 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E23605_2017
            SAMN12639989-rid9881463.denovo.096, whole genome shotgun sequence.
ACCESSION   DACDXO010000093 DACDXO010000000
VERSION     DACDXO010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639989
            Sequence Read Archive: SRR10023389
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741914.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:42:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,972
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 33 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 17 of 73
            Pseudo Genes (multiple problems)  :: 10 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6570
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639989-rid9881463.denovo.096"
                     /isolate="E23605_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            127..579
                     /locus_tag="IWM35_002526"
     CDS             127..579
                     /locus_tag="IWM35_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9920345.1"
                     /translation="MKKTILSIFSVFTILIGGYFLLNLDSVNSNAQIKDQRVAEHNNK
                     KMSLTKNIYSMSDEELYKEFGDLSGEQLEESIPGISDSDKIVVLSAGGQLMGDGKSEL
                     VSYKQNHRQFIDVNTDSNAISIKDLKELLNSKRNELKQIKETNSVELE"
     gene            593..1282
                     /locus_tag="IWM35_002527"
     CDS             593..1282
                     /locus_tag="IWM35_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9920346.1"
                     /translation="MKKIFGVSVGLLMMLTFGNAVYAAEKEVISDPEQYASRYFADFE
                     EEDKVLALPEGGFLKGEAKIVSMDNPDIIQGSYNSETDPASVSVTEAKKIVAEKFKND
                     IETTPIMPRGAFPPDVGTSGHLLVLAGGGSYKSQPFSGAGWRFGGMGVKPADGTGNFL
                     RWKSYIDDGRVGNVNQANATKSGSIQGMALYAREGFKYFAPNGPGWTMNIGIYYTYNP
                     VAGTYYVVQNI"
     gene            1407..3758
                     /locus_tag="IWM35_002528"
     CDS             1407..3758
                     /locus_tag="IWM35_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1906 domain-containing protein"
                     /protein_id="HAP9920347.1"
                     /translation="MDEMVLSTQKWLNKKYSNVTGFDKVPENGRTGWPTIYGLIEGLQ
                     VELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGINPGGFDGVY
                     TPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSKIRKMQQWLN
                     VNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGSYGNGTTSKTYPVSEGN
                     SGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTEIADVVVIKG
                     LLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGKDERNKYLTT
                     EELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIPLTTVIYFAV
                     DLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYASFAFVSDMS
                     TGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNELSNDTNNDNY
                     IAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIISSPVTEHDRK
                     IYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTINDSVSDFAGWL
                     GDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYTLAKRNSDST
                     ALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLENDETVDILGVGIISFFI
                     DKIVNNSLGTDFTYLDFIGDEGKVANGFSEKIIQFQENRSKLN"
     gene            3838..4233
                     /locus_tag="IWM35_002529"
     CDS             3838..4233
                     /locus_tag="IWM35_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9920348.1"
                     /translation="MMIMEEIEWNNLTQNNKNNSGCGIIFMFVIVLGIVVLLITVKIV
                     DYFANTPEEQIQRKLPEFERVPPYDPRDNSFQVKKLKESPNEMWFEVTVESSDFKEKK
                     KEYNYIVKAKVSKRFIFNPKLEIYSVVIE"
     gene            4558..5175
                     /locus_tag="IWM35_002530"
     CDS             4558..5175
                     /locus_tag="IWM35_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP9920349.1"
                     /translation="MRKFFYIRVSSYDQSIERQLISAKELGIPEEYIFVEKASGKDFK
                     RPEYQLMKRMFRKGDLLYLQSLDRLGRNKQMILEEWRELTKEKQIDISVLDMPLLDTA
                     SYKQLDGLETLISDLILQLLSYMAEDERKRINSRQREGVEAAKQKGVKFGRKKIALPP
                     EFPAIYSDWKAGKITAVYAMDQLGMKRNTFYRRVKEYEHTLHHFS"
     gene            5216..5515
                     /locus_tag="IWM35_002531"
     CDS             5216..5515
                     /locus_tag="IWM35_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9920350.1"
                     /translation="MDLTNQRVVFTGTLQQMTRTQAIGLVHSLNGHCQSSVTSKTNFL
                     VCGQYSKSLFDDSLYTKKEQTARDLIALGHEITILSEVEFYALISQQLKEWQRYL"
     gene            5549..5716
                     /locus_tag="IWM35_002532"
     CDS             5549..5716
                     /locus_tag="IWM35_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP9920351.1"
                     /translation="MKRKSYDRWGIEITSFNRNIAFHVECDCGELAKSVYQKNYFYCP
                     KCHRRYGVHGT"
     gene            complement(5761..6366)
                     /locus_tag="IWM35_002533"
     CDS             complement(5761..6366)
                     /locus_tag="IWM35_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP9920352.1"
                     /translation="MGKIGYIRVSSQDQNLDRQLAMMEGLNIDKLFQEKASGKDTERP
                     EFQKLLNYIREGDCVIVTSLDRLGRDYEDIKNTVAFMKQKKVALKILDAQFLDFNTGN
                     ELLDTAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAQKAGRYKGRPIEYSINSTDPQ
                     KRLVFKTVVDMLKQGIPVAEIAKENGISRPTIYKIKKANDL"
BASE COUNT         2265 a          910 c         1285 g         2110 t
ORIGIN      
        1 attcctcctg ctatcttttt ctattattct atcttatttg atagtagatt taaaactttg
       61 caacagaacc aattatctta tcgtagatat atattataaa tttaataaga aagcgaggtg
      121 agaatcgtga aaaaaacgat actaagtatt ttttcagtat ttacaatatt aattgggggc
      181 tattttcttt tgaatttaga ttctgttaat tcaaacgctc aaataaaaga tcaaagagta
      241 gctgaacata ataataaaaa aatgagtttg accaaaaata tctattctat gtcagatgaa
      301 gaattgtata aagaatttgg tgatttatcc ggggaacagt tagaagaaag tattccagga
      361 attagtgact cagataaaat tgttgtcttg tctgctggtg ggcaattaat gggagatggc
      421 aaatccgaat tagttagtta taaacaaaat cacagacagt ttattgatgt taatactgat
      481 tccaacgcta tttcgattaa ggatctaaaa gagcttttaa atagtaaaag aaatgaatta
      541 aagcagataa aagaaactaa ttcagtggaa ttagagtaaa ggaagattca ttatgaaaaa
      601 gatttttggt gtttctgttg ggctacttat gatgttaaca tttggaaatg cagtatatgc
      661 tgctgagaaa gaagttattt cagatccaga gcagtacgcc tcacgatact ttgctgattt
      721 tgaagaagaa gataaagtac ttgcattgcc agaaggtggt tttttaaaag gggaagctaa
      781 aattgtatct atggataatc cagatataat tcaaggttca tataattcag aaactgatcc
      841 tgcttcagtt tcagtgacag aagcaaaaaa aattgttgct gaaaaattca aaaatgatat
      901 tgaaactact ccaattatgc ctcgaggagc atttccaccc gatgttggta ctagcggaca
      961 ccttcttgta ctagcaggag gaggaagcta taaatcacag ccatttagtg gtgcgggatg
     1021 gcgttttgga ggtatgggtg taaaaccagc tgatggtaca ggcaactttt tacgttggaa
     1081 atcctacatt gacgatggaa gagtaggaaa tgtaaatcaa gccaacgcaa ctaaatctgg
     1141 ttctattcaa ggaatggcat tgtatgctcg tgaaggattc aaatactttg caccaaacgg
     1201 gcctggttgg acaatgaata taggtattta ttacacttat aatcctgttg caggtacgta
     1261 ttatgttgtt caaaatattt aataaaacag cacaaagaga tttctttgtg ctgttttatt
     1321 atttttagtt gttgacaaac actaaaatat aaaatataat caacacataa acgaacgtat
     1381 gttcggcaag aaagcagggg atttttatgg atgagatggt tttaagtact cagaaatggc
     1441 taaacaagaa atacagtaat gttacagggt ttgataaggt tccagaaaat gggcgaacag
     1501 ggtggcctac aatctatgga cttattgaag gactacaagt agaacttggt attactaatc
     1561 tagtagctaa ttttggacct accactgaaa aaatgtacga taatcaggtt actcctaaat
     1621 ggggcaaaaa tctacctaaa aatattgtat ttcttattca aggtgccttt tggtgtaagg
     1681 ggattaatcc aggtggcttc gatggggtat atactccata tttagatact gctgtaaaag
     1741 agttgcagac tgatgcaggg tttaatggaa gcgctgtaac tggagttttg gatgcgaaat
     1801 gggccaaagc gttatttgat atgagtgctt ttgttcttgt tcccggcgga gatagtaaaa
     1861 ttagaaaaat gcagcaatgg ttaaatgtaa attatttaga atacacggga ataatgcctt
     1921 gtgatggtat ttatcaaaga aattcaaatc aagcattaat ttttgcatta caagctgaac
     1981 taggatattc tcccagtgaa gcaacagggt cttatggtaa tgggacaact agtaaaactt
     2041 atcctgtgtc tgaaggtaac tcgggaaact atgtaagaat aattcaatat ggattatatg
     2101 ttaatggtta ttttgaagac ggtacattcg atggtatttt cactaaatat atgggtttag
     2161 aagtactggc attccgaaaa tttatggtat tgcctgaata tactgaaata gcagatgttg
     2221 ttgtaataaa aggactattg tctagcgctg gagatattag aagatctgca gatggcgcag
     2281 atacttctac gcaattaact agatctcaaa tccaaacatt ggttgataat gatataaaaa
     2341 tagttggtag atatcttact ggtactgttg gaattggaaa agatgaacga aataagtatc
     2401 taactacaga ggaattgaat aatatttttt ctaaaaattt atctgtgttt cctatctatc
     2461 aagatggagg tgcagcatta gcttatttta cttatgatcg tggcttatca gatggtaaga
     2521 aggctataga tgcagcaaaa aatctaaata taccattaac aacagttatt tattttgctg
     2581 ttgatctaga catgttaggg gaagaaattc ttgcatatgc tggagaatat tttaaggggg
     2641 tatctgctgt aatgtcatat agcgggtatc aaacgggtgt ttacggaaca agaaatgttt
     2701 gttctcaaat aatcaataac gggtatgctt cgtttgcatt tgtttcagat atgtcgactg
     2761 gttatagtgg gaatttaggt tttcctatgc ctagacaatg gtcttttgat caatttgttg
     2821 aatttaccat aggaagtggc aacggagctg ttggtatcga tttaatcgct acatcgggta
     2881 gagatagcgg atttaatgag ttatcaaatg atactaataa tgataattac attgccaaat
     2941 acaatcagaa ggttataaat cagatggtac gtgcgtatca atatctatca caagctggtc
     3001 ttgataatcc ttggaaccct catttgacat tttatagata tgtaaattat tctggtttgt
     3061 catgggacat catttcttct cctgtaacag agcatgatag aaaaatttat gatgaatata
     3121 ggaataaatt aactaatagc gagggcctgt ataattattt tatagatcct aatactggtt
     3181 caataatagg tttgcctcac ttaattgtga cgttacaatc acagatgttt ctagccgaca
     3241 caatcaatga tagtgtttct gactttgcag gttggttagg agatttaatg acttgctggg
     3301 gtgaagttaa gaaattagga atacaaatgg agcaaggtgt atttgcatta gtaggtacgg
     3361 ctgcaggtgg aacatttagt ctagaagatt tataccaaga tgttgacgcg gtaaatgttt
     3421 ataccttagc aaaaagaaat tctgattcaa cagctttatc ggctttaaaa acgtactatt
     3481 gtgataaggg tgctattgat agatttaatc aatttgttaa gaacagattt ggttcttcag
     3541 ataaaatcgc ttcagtaaca aatagtttat tagaaaatga cgaaacagtt gatatcttag
     3601 gtgtagggat aatttcattt ttcattgata aaatagttaa taattcactt gggacagatt
     3661 ttacttatct tgattttatt ggagatgagg ggaaagttgc aaatggattt tctgaaaaaa
     3721 ttattcaatt tcaagaaaat agaagtaaac ttaattaaga atcgtttact atttaaacag
     3781 tgtccttttc ctttttaaat atatacgata aaataagtat atgaattgta aaaggacatg
     3841 atgattatgg aagaaataga atggaataat ctgactcaaa ataataaaaa taattctgga
     3901 tgtggaatta tctttatgtt tgttatagtt ttgggaatag tagtactttt aataactgtc
     3961 aagatagtag attactttgc gaatactcct gaagaacaaa tacagagaaa acttcctgag
     4021 tttgaaagag taccacctta tgatccaaga gataattcat ttcaagtaaa aaaattaaaa
     4081 gagtctccaa atgaaatgtg gtttgaagtt acagttgaat caagcgactt caaagaaaaa
     4141 aagaaagaat acaattatat tgtaaaggca aaagtatcaa aaagatttat ttttaaccct
     4201 aaattagaaa tatatagtgt tgtaatagaa taatagtgtt aaaaaaattg taaaagttaa
     4261 agaagtcaaa ttctgatgtg aaaaataaaa tataccatgg aagacataaa agaaaaactg
     4321 gataactagt ttatttaatg gcagaccgtt aaggcctgcc atttttatgt attttagcgg
     4381 aataaatata atcatgcggg agtgtaaact tactgtttca aaaagtatac tttttgacat
     4441 gccttgattt tctttgttta tgtatggcat aataaccaca taatacaatt tgaaacgttt
     4501 ctgatttaat ctgattgttt tgaaacaatt ttagtgaaaa aagggtagat aaaaacgatg
     4561 agaaaatttt tttatatccg ggtcagtagt tatgatcaat cgatcgaacg acaactgatt
     4621 tccgcgaagg aattaggaat ccctgaagaa tatatatttg tagaaaaagc cagcggcaaa
     4681 gattttaaac gccctgaata tcaattaatg aaacggatgt ttcgaaaagg agatttactc
     4741 tatctacaat cattagatag actaggcaga aataaacaaa tgattttaga agaatggcga
     4801 gaattaacta aggaaaagca gattgatatt tctgtgttag acatgcctct gttagatact
     4861 gctagttaca agcaactaga tgggttagag acgcttatct ctgacttaat tctgcaatta
     4921 ctcagctata tggccgaaga cgagcggaaa cgaattaata gccggcaaag agaaggcgta
     4981 gaagctgcga aacaaaaggg tgtaaaattc ggcagaaaga aaattgcttt acctcctgaa
     5041 tttcctgcga tctattctga ctggaaagca ggaaagataa ctgcagtcta tgctatggat
     5101 caattgggca tgaaacgaaa taccttttat cgacgggtga aagaatatga acataccttg
     5161 caccactttt cttgatagat ataacagcta ttagacggga agggagagtt tgaaaatgga
     5221 cttaacgaat caaagggtcg tttttactgg aacgctgcag caaatgacac gaacccaagc
     5281 catcggtttg gttcactctt taaacggcca ttgtcaatca agtgttactt caaaaacaaa
     5341 tttcctggtt tgtggtcagt attctaagtc gttgtttgat gattctctct atacaaaaaa
     5401 ggaacaaacc gcgcgggact tgattgcgtt aggtcatgag attaccattt tatcagaagt
     5461 cgaattttat gcgttaatta gtcaacagtt gaaagaatgg caacggtatt tataatcgtt
     5521 agtaaaattt acaaggagga aaatttttat gaagcgaaaa tcttatgatc gttggggcat
     5581 tgaaataact agttttaatc gaaatattgc ttttcacgtg gagtgtgact gtggggaatt
     5641 agcaaaaagt gtttatcaaa aaaattactt ttactgtcct aaatgccatc gccgatacgg
     5701 ggtacatgga acatagaaac gaaatagtgc ggtagaaaag cgaatgaatg tagtatattt
     5761 ctacaaatca ttcgctttct ttattttata aatagttggt cgagagatcc cgttttcttt
     5821 tgcaatctct gcaactggaa tgccttgctt taacatgtcc accacggttt taaaaactaa
     5881 gcgtttttgc ggatctgttg agttaataga atactcgatt ggtcgtccct tatatcggcc
     5941 ggctttttga gccaataata ctccttgccg ctgacgttca cgaattttct cacgctcgtt
     6001 ttgtgcaata taacttagca gtgataaaaa catatcgaac atagcggtat ccaggagttc
     6061 atttccagta ttaaaatcta aaaactgtgc atctaatatt tttaacgcta cttttttctg
     6121 tttcataaaa gcaactgtat tttttatatc ttcataatct cttcctaaac gatctagtga
     6181 tgtaacgatg acgcaatcac cttcacggat ataatttagt aatttttgaa actcaggacg
     6241 ctcagtatct ttaccactgg ctttttcttg gaataattta tcaatgttaa gtccttccat
     6301 cattgccaat tgtcgatcca aattttgatc ttgtgaagat actcgaatat agccaatttt
     6361 tcccataaaa aactcctttt taaaagtgat ataatttaca tgtaaaattt acttgtagtt
     6421 ttactgctat catagcattt ttaaaaataa attttatgta gacttaaaat ttacttcatg
     6481 gagggtttaa atggatgaaa tcaaaagtaa caaaaaagca tttggcattt atttaggaga
     6541 acctagtaca gaacaattaa accaattttt
//
LOCUS       DACDXO010000094         6401 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E23605_2017
            SAMN12639989-rid9881463.denovo.098, whole genome shotgun sequence.
ACCESSION   DACDXO010000094 DACDXO010000000
VERSION     DACDXO010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639989
            Sequence Read Archive: SRR10023389
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741914.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:42:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,972
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 33 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 17 of 73
            Pseudo Genes (multiple problems)  :: 10 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6401
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639989-rid9881463.denovo.098"
                     /isolate="E23605_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..177
                     /locus_tag="IWM35_002534"
     CDS             <1..177
                     /locus_tag="IWM35_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9920353.1"
                     /translation="QSLVLLAIVVIGLFLGFKREFSKLIGFLVIALIAVGLVFNASGV
                     KDVLLNLFNRIVGA"
     gene            190..690
                     /locus_tag="IWM35_002535"
     CDS             190..690
                     /locus_tag="IWM35_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HAP9920354.1"
                     /translation="MEQIRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELKGTPIGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCNDMSDVAYYLIVEAGDLGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFELM"
     gene            787..2634
                     /locus_tag="IWM35_002536"
     CDS             787..2634
                     /locus_tag="IWM35_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HAP9920355.1"
                     /translation="MVGNKWYKCDLHIHSTASQCFQDKKVTAKEWVEECLNKGLDCVA
                     LTDHNTGENIDEYKKVAIENGLDFFPGVELTCGENGTHLLVLFDKSACTNDISDFLTK
                     MGIKREDWASAAACSMKSVLEVIDFAEHEGLLVIPAHIDEFNGISSAKFGNLGKIFES
                     ENIYAVQTVQKEFFENRSQMIPSSKRDTIYDSVNARYDGRVGKDTLESWYKSVVEAEK
                     NNMTFLSFSDNPHSKGNSKHGLWGIGTRYSYIKMKDEPDLGSLRDALMLGETRVHSDF
                     SNFSINENEVLLEKLSFSGTTLSTKEVVVEFSDNLTSIIGGRGTGKSCITRFLVYVLG
                     KEAELDQFSEIQSDYQNFAQIEHNGSGIFLKDTIVKLNIFYKGTKYQIIRTQDRHSIY
                     EFTQENGLVEKETERLRMISDKVSIYSQKQIYEISKNQTSILELLDGYNSDLISEYKN
                     EIETCVNEIRKLNWDIVSVKKEIQDKAKVELEIEDLKKQVEKLSHKSYKDVYDEYSKE
                     TDIYRELKRDVEALKEIPKNLTDSVDKIDFGNGIKVTDEIDEHRGELIKNLVTNRAKI
                     QTIINEMSDEIDSYRAELNKSDWKVNYNEVSKRYQRGIKNLVKSYLKMN"
     gene            2637..3533
                     /locus_tag="IWM35_002537"
     CDS             2637..3533
                     /locus_tag="IWM35_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP9920356.1"
                     /translation="MLQNLDSLYQSLSKTEERLEEIIQQEIKLKDYQVEVDSAMSRMN
                     KSYQNLYSERSSSSREIFSDITGIEATIQPNRDFNNYVKELRACINKEDTFDSEFGSL
                     IDKLTKNSITNQDIYNDILDIRVSGESSIFSDKKLINSIRKLNDAQVLELQTLKPNDL
                     VVIRLELNGKKVSLSNASAGQKTSAILSMILAYGNSPLILDQPEDDLDSQLINNLIVR
                     SIITKKENRQILIVTHNANIPVNGDSEWLISMGDSKEISADQCGSVDEPNIKPRICTV
                     MEGGEDAFTNRAKRYGFKQIVE"
     gene            3583..3972
                     /locus_tag="IWM35_002538"
     CDS             3583..3972
                     /locus_tag="IWM35_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP9920357.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEEVFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPSTLVRSVNYVSD"
     gene            3959..6304
                     /locus_tag="IWM35_002539"
     CDS             3959..6304
                     /locus_tag="IWM35_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAP9920358.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELVPYNYSFLSPEQKFQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLKEVAKQRIDIQTDALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKSLKKSFFSGLQEFVYGVNHHLMGDFVSLSNEEI
                     RCYSKLEKLMESKLARRFKVRRVTPSDLTYLIEHIYGEKGIPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPNRCLIEEKPRCLRMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKGSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEISILNLTSKSENQGLLDPYVIMKKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSDQRGLLQVINELRKDGSPIAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDKQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDQEDQSNQKRLRDLENGITSI"
BASE COUNT         2161 a          950 c         1370 g         1920 t
ORIGIN      
        1 caatctttag tcttactagc gattgtggtc attggtttgt ttttaggatt taaacgcgag
       61 ttttccaaac tgattggctt cttggtgatt gctttgattg cggtaggtct ggtctttaat
      121 gcttctggtg tgaaggatgt cttactcaat ttgtttaacc gaattgtcgg tgcctgaggg
      181 ggaatcacaa tggaacagat acgtgtttat attgcgaacc ttggaaaata caatgaaggt
      241 gagttggtgg gggcatggtt taccccaccc gtcgattttg atgaagtgaa agaacggatt
      301 ggattaaatg acgaatatga agagtacgca attcatgatt atgaactgcc ttttgagatt
      361 gatgagtata caccaattga ggaaattaat cggttgtgtg gtttagcgga agagcttaaa
      421 ggaacgccaa ttggtgaggt cgcttcagag attcaacatg ccttcttcaa ttctttcgag
      481 gaaatggtag agcatgtgga tgatatcatc tattatcccg attgtaatga catgagtgat
      541 gtagcgtatt acttgattgt agaagctggt gatttaggcg aagtaccaga acacttgaag
      601 acgtatattg attttgatgc gtgtggtcga gacttggaga ttggcgggaa ttatttggtt
      661 acgagtcgag gggtttttga actaatgtaa atcatttgtt agaattgacc agaaatttct
      721 atcgtacttt tacttttcta tttgatatac tgaaatagaa aatatttgtt gataggggtg
      781 ttactaatgg ttggcaataa gtggtacaaa tgtgatcttc atattcattc aactgcgagt
      841 cagtgctttc aggataaaaa ggttactgct aaggaatggg tagaagaatg tttaaacaaa
      901 ggactcgatt gtgttgcatt aactgatcac aatacagggg aaaatattga tgaatataag
      961 aaagtggcta tcgaaaatgg cttagatttt tttcctggag tggaattaac gtgtggagaa
     1021 aatggaactc atttactagt cctatttgat aaaagtgcat gtacaaatga tatctctgat
     1081 ttcttaacca agatgggtat aaaaagagag gattgggcca gcgctgcagc ttgctcaatg
     1141 aaatcagttt tagaagttat tgattttgct gagcacgagg gcttgctcgt tattcctgca
     1201 catatagatg agtttaatgg aatttcttca gcaaaatttg gaaatctagg aaagatattt
     1261 gaatcagaaa atatatatgc agtacagacg gttcaaaaag aattttttga aaatcgctca
     1321 caaatgattc cctcatctaa gcgtgacact atctatgata gtgtgaatgc cagatatgat
     1381 ggtagagttg gaaaagatac tttggaatct tggtataaat cagttgttga ggcagaaaaa
     1441 aataatatga catttcttag cttttctgat aacccacact cgaaaggaaa ttcaaaacat
     1501 ggactttggg gaataggcac aagatattcc tatataaaaa tgaaggacga acctgacttg
     1561 ggatcacttc gcgatgcttt aatgctaggc gaaactcgag ttcattctga tttttctaat
     1621 ttctccatta atgaaaatga agttttattg gaaaaattaa gcttttctgg gaccactctt
     1681 agcacaaaag aagttgtagt cgaattcagc gataatttaa cttcaattat tggaggaaga
     1741 ggaacaggca aatcttgtat tacaagattc ttggtctatg tccttggaaa ggaagcagaa
     1801 cttgatcaat tttctgaaat acaaagtgat tatcagaact ttgcacaaat tgaacataat
     1861 ggctctggaa tatttttgaa ggatacaatt gtgaaactga atattttcta taaggggaca
     1921 aaatatcaaa taataagaac acaggataga cattctattt atgaatttac acaagagaat
     1981 ggtctggttg aaaaagaaac agaacggttg agaatgattt ccgataaggt gagtatatac
     2041 tctcaaaaac aaatttatga aatttcaaaa aatcaaacta gtatactaga attactagat
     2101 ggatataact ctgatttgat ttcagagtat aaaaatgaga ttgaaacatg tgtaaacgaa
     2161 ataagaaaat taaattggga tattgtttct gtgaaaaaag aaatacaaga taaggcgaag
     2221 gtagagcttg aaattgaaga tttaaaaaaa caagtagaaa agctatctca taagagttat
     2281 aaagatgttt atgatgagta ttcaaaagag acagatattt atagggaact gaaacgtgat
     2341 gtggaggcac ttaaggaaat tcccaaaaat ctaactgata gtgttgacaa aattgacttt
     2401 ggcaatggta taaaagttac agacgaaatt gatgaacacc gcggagaatt gattaagaat
     2461 ttagtgacca atcgggctaa gattcaaaca ataattaatg aaatgtcaga tgaaattgat
     2521 agttatagag cagagctgaa taaatcagat tggaaagtta actataatga ggtttcaaag
     2581 aggtatcaaa gaggtatcaa aaacttagtg aagagttatc tcaagatgaa ttaaaaatgt
     2641 tacaaaacct tgattcttta tatcaatctt tatcaaaaac tgaagagcga ttggaagaaa
     2701 ttattcaaca agaaataaag cttaaagatt accaagttga ggttgactca gcaatgtcaa
     2761 gaatgaataa atcatatcaa aatctctatt ctgagagaag tagttcctcg cgcgaaattt
     2821 tttctgatat tacaggaata gaggctacta ttcaaccaaa tagggatttc aataattatg
     2881 ttaaagagct cagagcttgt attaataagg aggacacttt tgacagtgag tttggatctc
     2941 ttattgataa gctgacaaaa aattccatta ctaatcaaga tatatataat gacatacttg
     3001 atataagagt tagtggggaa agtagtattt tttcagataa aaaattaatc aattctataa
     3061 gaaaattgaa tgatgcacaa gttcttgaat tacaaaccct taagccaaat gatttagtag
     3121 ttatccgact tgaactaaat ggaaaaaaag ttagtttatc gaatgctagt gctggtcaaa
     3181 agacatctgc aattctttct atgattttgg cgtatggaaa ttcaccactc atattggatc
     3241 agcctgaaga tgatttggat agtcaattaa ttaataactt aatcgtaaga agtatcatta
     3301 caaaaaagga aaacagacaa attttaattg taactcataa tgcaaatatc ccagttaatg
     3361 gtgattcaga atggttgatt agcatgggag attcaaaaga aatatcggct gatcagtgcg
     3421 ggtcggtaga tgaacccaat atcaagccaa gaatatgtac tgtgatggaa ggtggcgagg
     3481 atgcttttac aaatagagcg aaacgctatg gttttaaaca aattgtagaa taacactaga
     3541 gggtgagaca tattattatg tccgcccttt ttaggaggaa aaatgaaaaa aataaaaagc
     3601 tataccagta tctgggcagt tgaaaaagtc atttacgcaa tcaatgattt tcagttgcct
     3661 tttcctgtga cttttaacca aatggcttgg ttcgttttat ctttactgtt tgtcatcgta
     3721 tttgcccatg tgcctccact atctatgata gaggaggtct ttttaaaata cttaggcata
     3781 ccggtggctg tcacttggtt tatgtcgcaa aagacgtttg atggcaagaa accactggga
     3841 tttttacgat cctttatcag ctatcacttg cgatttaagg tgacttttgc aggaaagaaa
     3901 gtcaaagaac agaagaaacg atgggatgaa cccagtactc ttgtaaggag tgtgaactat
     3961 gtatccgatt aagtatattg aaaataatct cgtctttaac caagaaggcg agtgttttgc
     4021 ctactatgaa ttagtgcctt acaattactc ctttttgtca cccgaacaga aatttcaagt
     4081 gcatgataac tttcgccaac taattgcgca aaatcgtgaa ggcaagatcc atgcgttaca
     4141 aattgccaca gaaagtagta ttcgcgccac acaagaacga tcaaaaaaag aaattaccgg
     4201 tagactcaaa gaagtagcga aacaacgaat tgatatacag acagatgcat tagtatccat
     4261 gattggcgat agtcagattg attatcgctt ttttattggc tttaaactaa ttgcgacaga
     4321 tgaagaagtc aatctaaaga gtctcaagaa atctttcttt tctgggcttc aagaatttgt
     4381 ctatggagtg aatcatcatt tgatgggcga ttttgtttcc ttatccaatg aagaaattcg
     4441 ctgctattct aagcttgaaa aattaatgga aagcaaattg gctcgaagat ttaaagtcag
     4501 gcgagtcaca ccgagtgacc tgacgtattt gattgaacat atttatggcg aaaaagggat
     4561 accgtttgaa gaatacgaat ttcagctacc aaagaaaaag ctaaaatcag aaacactggt
     4621 gaaacgttat gacttattac gtccaaatcg ttgtttaatt gaagaaaaac caaggtgctt
     4681 acggatggaa catgagaatc atgagtcgta cgtagcgtat ttaacaatta atacaattgt
     4741 gggggagatg gaatttcctt cctcagaact tttctattac cagcaacaac aatttacatt
     4801 cccaatcgac acaagtatga atgtagagat tgtgacgaat aaaaaggctt tagctaccgt
     4861 tcgtaataag aaaaaagaac tgaaagattt agacaatcat gcttaccaat ctgacaatga
     4921 aacgaattcc aatgtcttag atgctttgga ttccgtggat gaattggaaa ccaccttgga
     4981 tcagtcaaaa gaatctatgt acaaactgag ttatgtggtt cgagtgagtg ccgaatcagt
     5041 ggatgaacta aagcgacgct gtgatgaagt gctcgatttt tatgatgata ccaatgtaaa
     5101 actagtccgg ccctttggcg atatgatggg gctacatgaa gaattcttac cctcaagtaa
     5161 acgatatatg aacgactata ttcagtatgt tacgtctgat tttttggcgg ggcttggttt
     5221 tggggctact cagatgctag gagaattaga agggatttac tttggctaca atgtggatac
     5281 aggtcgtaat gtttacctga aaccggcatt ggcttcacaa ggagtcaagg gttcagtaac
     5341 caatgcgctg gccgccgctt tcctgggttc tcttggtgga ggtaaatcct ttagtaacaa
     5401 tctcttagtc tactatgctg ttttgttcgg tggtcaagct gtgattgtcg atccaaaagg
     5461 ggagcgagga gggtggaaag aaaccttgcc agaaattgcg gatgaaatca gtatcctcaa
     5521 cttgacgagt aagtcagaaa atcaaggtct gcttgatcct tatgtgatta tgaagaaaaa
     5581 gaaagactcg gaaagtttag caattgatat tctgactttt ttaacaggaa tttcaagtcg
     5641 agacggggag aagtttcctg tgttacgtaa agccattcga acggtgactc aaagtgatca
     5701 acgggggtta ttacaagtta ttaacgaatt gcgaaaagac gggtcaccaa tcgcggaaaa
     5761 tatcgccgat catatcgagt cctttaccga ttatgatttt gctcatttgc ttttctcaga
     5821 cggctcgatt acccaatcga ttagtttaga taaacagcta aatatcattc aagtggcgga
     5881 tttggtttta ccggatgcag aaacgacttt tgaagaatat accaccatgg aattgcttag
     5941 tgtttcgatg ctaatcgtca tttccacttt tgccttggac tttatccata gtaaccgaga
     6001 aatcttcaaa attgtcgatt tggatgaggc ttggagtttt cttcaagtgg ctcaaggaaa
     6061 aacgttatcg aataagcttg ttcgtgctgg tcgggccatg aatgctgggg tctatttcgt
     6121 cactcaaaat agcgacgatt tattagatga acgtttaaaa aataatatcg ggttgaagtt
     6181 tgcttttcgc agtaccgata ttcatgaaat caagaaaacc ttggaattct ttggtttgga
     6241 tcaggaggat caaagcaatc aaaaacgcct gcgagatttg gaaaacggga ttacgtccat
     6301 ataattgtgt aaaagtgaag catcaaaaaa gccattgatc tcagttagga gtacaatgtt
     6361 ttttgcagaa aacatactcg aataggactg atatcaatga c
//
LOCUS       DACDXO010000095         6303 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E23605_2017
            SAMN12639989-rid9881463.denovo.099, whole genome shotgun sequence.
ACCESSION   DACDXO010000095 DACDXO010000000
VERSION     DACDXO010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639989
            Sequence Read Archive: SRR10023389
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741914.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:42:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,972
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 33 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 17 of 73
            Pseudo Genes (multiple problems)  :: 10 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6303
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639989-rid9881463.denovo.099"
                     /isolate="E23605_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            334..714
                     /locus_tag="IWM35_002540"
     CDS             334..714
                     /locus_tag="IWM35_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HAP9920359.1"
                     /translation="MSEQHQNLASDHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVAPD
                     DAQRLSENLKIMQKELQKIWQQLK"
     gene            696..1610
                     /locus_tag="IWM35_002541"
     CDS             696..1610
                     /locus_tag="IWM35_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HAP9920360.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     TLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPNHQIAIYTHTDKDHIHN
                     HIVINSVNLETGKKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKTEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYTQFCDSLKTFGIHCLNRGKNFTYELVHKKK
                     KVRSNKLGKDYEKETILHELDRRERTYNQQRTNELRRARASIRETAEEVRREQRTFTP
                     EQSEIRPTRRNHEQHVERGTDHQQELSL"
     gene            1855..2544
                     /locus_tag="IWM35_002542"
     CDS             1855..2544
                     /locus_tag="IWM35_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9920361.1"
                     /translation="MAFYLPYLLISASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLV
                     GVIFALSIVLSGLFGVLEGNKRVSRSILLGDITQKYEAACDKKKKEQALAQKMEEFTA
                     CYEDMNNIFVDQEKRLTDKNMKKLTRFYQNLPEKQQKEVQEKYEQVKKDVQYFKDTQT
                     EEACCNLFSDTIPFSTSEQERKERQQSVTYERYETLLQQATNIQNPTKKETALNYLES
                     VKEWLDQQQQN"
     gene            complement(2665..2751)
                     /locus_tag="IWM35_002543"
     CDS             complement(2665..2751)
                     /locus_tag="IWM35_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011302749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HAP9920362.1"
                     /translation="MFERFICPLLVEVCVSLFSYWLNNRNKK"
     gene            3427..4377
                     /locus_tag="IWM35_002544"
     CDS             3427..4377
                     /locus_tag="IWM35_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HAP9920363.1"
                     /translation="MANEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMREKGDKTVR
                     LTFDQLKDLSNYKATANNRFINDLEMTYDKLMDLRFGKRSANGLDIDRFVMFSEFKIR
                     GTAKDPYVDIQVYEKALPLLNNLDEWVRYSLQQFNELQSSYAKTMFRLLKQYRTKGFA
                     YFSKEDFHELLDIPKSYWNNQANVDNRVIKPIRQELTAIFKGLSIKKKYGKGRGKPVV
                     GYQFTFKPEIKKADDFNKHAPTKEEQNKALALADKQKEYEEPATTINEKIKQKKRELE
                     LLQQIKQLEKEKEALEKQTESALSKKEKKSLLSDLDNLFQ"
     gene            4398..4931
                     /locus_tag="IWM35_002545"
     CDS             4398..4931
                     /locus_tag="IWM35_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HAP9920364.1"
                     /translation="MQPQETFTIKELSELFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLGNQMLLDRLHEYEQDNDKNVNIPSKNVVTNKLLDQLREENEFLRE
                     QLALREQNNSHIQKLLDQQQQLTLQANKQIEKLQEKLQLTYTEEAPENDSTTLSEATE
                     TIDKQIDTEKKWWQLWK"
     gene            5567..6100
                     /locus_tag="IWM35_002546"
     CDS             5567..6100
                     /locus_tag="IWM35_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9920365.1"
                     /translation="MKKFTLGVALLTIGAVVLPSTTIVYASENSQVVSLNENNATENV
                     VIEYTEDGMVVTKYEDGYSAEDFANSSDIMTRAAVARWGKKEYTNIAVTTGVAANAIN
                     AAFWAGGKASTSMFGIPGWAIGALLTGASWTKKGASPGNAVAKEWDKNGNGWIGFYYQ
                     RGYDAAGRVVATRYSTL"
BASE COUNT         2323 a         1102 c         1174 g         1704 t
ORIGIN      
        1 aaaaaaaaat aaatcgatac attttagaag aatagggttg tttgaatatt acaaagcaac
       61 aatttcacct tcggtgaaat aagcgaaact ttacaaaaag tggctagcaa atgctagcct
      121 ttttttgtat tgtggagcgt ggattttgag ggggattcaa gggggagctt gcgctccttt
      181 gacaagcggt tatttgcacc aaatacggag gtatttggct agcaaatata gagcttgcca
      241 aaccctttta gtgagcttta ctcattgtac ctttttaggt acaatggtat tgcgaacgat
      301 acgtccgtag gacgtactga aagggtggga cttatgagcg aacaacatca aaatttggct
      361 agcgatcatt ctaaaaaata tacctatcga tccgagccaa aacaaatcag ttttcgagtg
      421 agcgaatccg aatttgcaaa gctgaagcag tcagctgaag ctttgcaaat gagtgtgccg
      481 gctttcgtga aagccaaggc acaaaacgcg cgtctggtga caccgaaagt ggcgccagac
      541 attgcccagg caatcgcgcg cgatttagcc aaagctggag ggaatatcaa tcaaattgcc
      601 aagtggtgca atacacacca acacgacgtt gctcctgatg atgctcaacg tttatccgaa
      661 aacctaaaaa tcatgcaaaa ggagctacaa aagatatggc aacagttaaa gtaagtcgaa
      721 ccacttcatg tagccgagca atcaattacg cagaaccacg agcaaccgtc aaaacaggaa
      781 taaactgtga cattgattat gccaaaagtg aaatgaaaca aatacgcacg ctttatggaa
      841 aagacgatcg tgtacaagca cacttattga ttcagtcctt tcgaccagga gaaattaccg
      901 ctgaaaaagc caatcaacta ggggcagagt atgcgaaaaa aatagcccct aaccaccaaa
      961 tcgcgattta cacacataca gacaaagacc atattcataa tcacattgtc attaactccg
     1021 ttaatcttga aactgggaaa aaatttcaag cccatgggca agcattttta aacaaatgtt
     1081 atgacctaaa tgacgagatt tgtcttgaac atgacttaac aatcacggaa cgaggaaaga
     1141 aaaccgaaaa acgaaccatg tctgaaatta aattaaaaga aaaaaatgaa ccggtttgga
     1201 aagacgaaat tcggtttgcg attgatcaaa caatgaaaaa caaaaaaact cgaacgtata
     1261 cccaattttg tgattcactt aaaacattcg gcattcattg cttgaatcgc ggaaaaaatt
     1321 ttacttacga attggtccac aaaaagaaaa aagtacgatc caacaaacta ggaaaggatt
     1381 acgaaaagga gacgatttta catgagctgg acagacgaga acgaacctac aatcaacaac
     1441 gaaccaacga gctacgaaga gctcgagcta gcattcgcga aactgcagaa gaagtacgaa
     1501 gagaacaacg aacttttacg cctgaacaat cggaaattag accgactcga agaaaccatg
     1561 aacaacacgt tgaacgagga acagaccacc aacaagaatt atcgctctaa aacgttaaaa
     1621 gacatcgaaa acgaaataaa gagacaacgt gcctttcttg cgccttatac ccaagaaagg
     1681 atacaaacta cgatggattc acaagtcaaa aaactccacc aagcgctatt agaccagcga
     1741 aaccaagaac aaaaaaaggc tttaattcaa cgatcgatgg atattcttgc ccccttatgt
     1801 tgcttagtac taacattatt agccttttgg ttggtatggg gagaataaac agacatggca
     1861 ttctatttac cctatctact catttctgcc agtattttag gaattatttg gcttagctat
     1921 aaggcttatc aaacaagata ttcattaaaa ggctctaaaa ttcgatttaa gcgattttta
     1981 ttggtcgggg ttatatttgc cttatcaatc gttttaagtg gtttattcgg cgttttagag
     2041 ggtaataaac gtgtttcaag aagcatttta ttaggggata tcactcaaaa gtatgaagca
     2101 gcatgtgata aaaagaaaaa agagcaagca ttagcacaaa aaatggagga atttaccgct
     2161 tgttatgagg acatgaacaa tatttttgtg gatcaagaga aacgtttaac agataaaaac
     2221 atgaaaaaac tcactcgttt ttatcaaaac ttaccagaaa aacagcaaaa agaggtacaa
     2281 gaaaaatatg agcaagtaaa aaaagacgtg caatatttta aagatacgca gacagaagaa
     2341 gcttgttgta acttatttag cgatacaatc cctttttcaa ccagtgaaca agaacgaaaa
     2401 gaacggcaac aatcggtcac atacgaacgt tatgaaacac tgcttcagca agcaacaaac
     2461 atacaaaatc caacgaaaaa agaaacggca ttaaattatc tagaaagtgt caaggaatgg
     2521 ttggatcagc aacaacaaaa ttgaatttga ttagcaatca tgttaaactg aacctgcaaa
     2581 tgatcaaaca ttagatacac gaaagcccca gagaggtcgg attctctggg gctttttttc
     2641 aggttgatgt cgcctttatt gattttactt cttgtttcgg ttattcaacc aataactgaa
     2701 taaagagaca caaacttcta caagaagggg gcaaatgaac cgttcaaaca ttagatacac
     2761 ctccttccag tcgcaagact gtcggatagg cgacaagtta ttatacctta caaaataaaa
     2821 tctgtgtggt gtttttaagt atcccttatt ttcgggttgt tagggcttgc cctgaccgcc
     2881 tgtaaggcgc tttaagaaca agataaggtt ttttacccaa gactgtctta aaacgactta
     2941 gacgagctat cagaggcttc ccccacctcc gttactgact tgtttttagc aaaggagcct
     3001 ttaggcgact gcgcagtaca atgtgagagc atagcgatca cactttttta gcgaccgtag
     3061 ggagcaaggt tttgaattgg cagttttgaa aaatgcgcga acctgttggt agaaaaaaga
     3121 agtggttttt aggcgggaaa ttttgtcacc ctcttttata atctcttttt aaaccttttt
     3181 ttaaacctct ttaccgtcgg ctgtgtcctt actccttcaa tggatacagc gttttataaa
     3241 ctacattttg tctgctttta aactacattt tgtctgcttt taaactacat tttgtctgct
     3301 tttaaactac attttgtctg cttttaaact ctttttttgc actcacgtat gttataatat
     3361 gcattatcac agacaaaacg ttgtttataa cgtctgtatc cattgaagga gtaaggagga
     3421 atatctttgg caaatgaaat cgtgaaatac catcatgaat taaataccat tccgttgcgg
     3481 aaatttacac cagttgaaat gaatctattt ttctctattg tgtctagaat gcgcgaaaaa
     3541 ggcgataaaa cagtccgtct tacttttgac caactaaaag atttaagtaa ttataaagca
     3601 actgctaata atcgtttcat taatgattta gaaatgactt atgataaatt aatggattta
     3661 cgttttggta aaagaagtgc taatgggctc gatatagata gattcgttat gtttagtgag
     3721 tttaaaatac gtggaactgc taaagatcca tatgtagaca tacaagttta cgaaaaagct
     3781 ttacctttac taaacaattt agatgagtgg gttcgctatt cactccaaca atttaacgaa
     3841 ttacaaagta gctatgcaaa gactatgttt cgattactaa aacaatatcg aactaaaggt
     3901 tttgcttatt tttctaaaga ggattttcat gaactattag atattccaaa aagttactgg
     3961 aacaaccaag caaacgttga taatcgtgtt ataaagccta tacgacagga attaaccgca
     4021 atcttcaaag gattatcaat caaaaagaaa tacggaaaag ggcgaggaaa acctgtcgtt
     4081 ggctatcaat ttacgtttaa accagaaatc aaaaaagcag atgattttaa taaacatgca
     4141 ccaaccaaag aagaacaaaa taaagcattg gctttagctg ataaacaaaa agaatacgaa
     4201 gaacctgcta caacaattaa cgaaaaaatc aaacagaaga aacgagaact tgaactcctc
     4261 caacaaatca aacaactaga aaaagagaaa gaagctcttg aaaaacaaac agagtccgca
     4321 ctttcgaaaa aagagaaaaa aagtttatta tctgatttag ataatttatt ccagtagcaa
     4381 cctaggaggc aaccagtatg caaccacaag aaacctttac aataaaggaa ttgtcagaac
     4441 tttttaagat gagtaggcaa gcaatatcga aacatatcca aaaattagat tcttccatga
     4501 tagctaaaaa tgagcgtggt tataaggttg tattgcgttc tggagtattg caacttgctc
     4561 gcaacttagg caaccaaatg ttgctagata ggttgcacga gtacgaacaa gacaacgata
     4621 aaaatgttaa tataccaagc aaaaacgtgg ttacaaacaa gttgctagat cagttgcgtg
     4681 aagaaaatga atttttacga gaacaacttg ccctcagaga gcaaaataat tctcatatac
     4741 aaaaactact cgaccagcaa caacaactca ccctacaagc caacaaacaa atcgaaaaac
     4801 tgcaagaaaa actacagttg acttatacag aagaagctcc agagaatgac tcaacgactc
     4861 tttctgaagc aacagaaaca atagacaaac aaattgatac tgaaaaaaaa tggtggcaac
     4921 tttggaaata actggaggga acattcatga caagaaaaaa tcttctattc ggttctattt
     4981 tactaggaat tctaggattc agtccgatgt tattgtcagt gatcaataaa gggaataaac
     5041 atggtgaatt gaaattgaat ccaaattatt acaacagtcc taacgaagaa ttgtttgtaa
     5101 aagaaacaga taaataataa aaaagactgc tctttcgagc agccagttaa aaaataatga
     5161 gtgtttattc ttgaaaaagc aacgcgctta tgaagcgcta ccaaaaattt atcatatttt
     5221 attaaaaata acaacccagt aaaaaatgac tggtaaaaat tcacaaagaa acggaaagaa
     5281 gcaaccgcag aaaatcaagc ttaaaaagag cttttgacct agaccaatcg aaaatcacaa
     5341 agtgagcgcc tgtatctatt gctacaggcg ctacttggca tagtgtacat tatgtcaaca
     5401 agaatttaat ataacatatt ttcctaatgc attctaacta taccacatta tcaaaaatat
     5461 aaattatctt tactaaataa aatattgaca agcctatacc ttttgtttta tgattttcac
     5521 aaagaaaacg ttttctatat atttttctgt atcaggagga aattctatga aaaaatttac
     5581 tttaggggta gctttgttaa ctattggggc agttgtttta ccgtcaacta ctatagttta
     5641 tgcttcagaa aattcccaag tggtatcatt aaacgaaaac aatgctactg aaaatgtagt
     5701 tattgagtac actgaagatg gtatggtcgt aactaaatat gaggatggtt atagtgcgga
     5761 agattttgca aattcttcag atatcatgac aagagctgca gttgcaagat ggggcaaaaa
     5821 ggaatataca aatattgctg taaccactgg agtagctgct aatgctatta atgctgcctt
     5881 ttgggcagga gggaaagcta gcacttctat gtttggtatc ccaggttggg caattggtgc
     5941 tttgttaaca ggtgcaagtt ggactaaaaa aggtgcttct ccagggaatg cagttgcaaa
     6001 agaatgggac aaaaatggta atggctggat tggattttat taccaaaggg gatatgatgc
     6061 agctggaaga gttgtagcta ctagatattc aacgttataa gttaaggagt gatgtttttg
     6121 atatctaaat cattaaaatc attaaaaaca tttactaaaa agaatccgca aatagctact
     6181 attcgtattt ttatatttgc tatagctgtt gctataattg ttaatatgct aagcggaggg
     6241 caattataca aatctggttt atttttagtg ttaatcttag ttgtcttaga cataataaag
     6301 tct
//
LOCUS       DACDXO010000096         6173 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E23605_2017
            SAMN12639989-rid9881463.denovo.100, whole genome shotgun sequence.
ACCESSION   DACDXO010000096 DACDXO010000000
VERSION     DACDXO010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639989
            Sequence Read Archive: SRR10023389
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741914.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:42:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,972
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 33 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 17 of 73
            Pseudo Genes (multiple problems)  :: 10 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6173
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639989-rid9881463.denovo.100"
                     /isolate="E23605_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            725..811
                     /locus_tag="IWM35_002547"
     CDS             725..811
                     /locus_tag="IWM35_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011302749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HAP9920366.1"
                     /translation="MFELFICPLLIEVCVSLFSYWLNNRNKK"
     gene            complement(932..1621)
                     /locus_tag="IWM35_002548"
     CDS             complement(932..1621)
                     /locus_tag="IWM35_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9920367.1"
                     /translation="MAFYLPYLLIFASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLP
                     GVIFTLSIVLSGLFGVLEGNKRISRSILLGDITQKYEAACDKKKKEQVLTQKMEEFTT
                     CYEEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYFKDTQT
                     EEACYNLFSDTIPFSTSEQERKERQQSVTYERYKALLQQATNIQNPTKKETALNYLKS
                     VKEWLDQQQQN"
     gene            complement(1628..1906)
                     /locus_tag="IWM35_002549"
     CDS             complement(1628..1906)
                     /locus_tag="IWM35_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9920368.1"
                     /translation="MKEEQTTNKNYRSKTLQDIENEIKKQRAFLAPYTKERIQTTIDS
                     QVKKFHHALLNQRNKEQKKVIMQRSMDILAPLCCLIMTLLAFWLVWGE"
     gene            complement(1866..2780)
                     /locus_tag="IWM35_002550"
     CDS             complement(1866..2780)
                     /locus_tag="IWM35_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HAP9920369.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHN
                     HIVINSVNLETGNKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYNQFCDSLKTFGIHCLNRGKNFTYELIHKKK
                     KVRSNKLGKDYEKETILHELDRRERAYNQQRTNELRRARASIRETAKEVRREQRTLTT
                     EQPEIRPTRRNHDQHVERGADYQQELSF"
     gene            complement(2762..3142)
                     /locus_tag="IWM35_002551"
     CDS             complement(2762..3142)
                     /locus_tag="IWM35_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HAP9920370.1"
                     /translation="MSEQNQYLASNHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVDPG
                     DAQRLSENLKIMQKELQKIWQQLK"
     gene            complement(3592..3879)
                     /locus_tag="IWM35_002552"
     CDS             complement(3592..3879)
                     /locus_tag="IWM35_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HAP9920371.1"
                     /translation="MDFTKEEKLLNAISKVYNEATIDDYPDLKEKLFLYSKEISEGKS
                     VGEVSMKLSSFLGRYILKHKFGLPKSLIELQEIVSKESQVYRGWASIGIWS"
     gene            complement(3899..4123)
                     /locus_tag="IWM35_002553"
     CDS             complement(3899..4123)
                     /locus_tag="IWM35_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin hiracin-JM79"
                     /protein_id="HAP9920372.1"
                     /translation="MKKKVLKHCVILGILGTCLAGIGTGIKVDAATYYGNGLYCNKEK
                     CWVDWNQAKGEIGKIIVNGWVNHGPWAPRR"
     gene            complement(4578..4793)
                     /locus_tag="IWM35_002554"
     CDS             complement(4578..4793)
                     /locus_tag="IWM35_002554"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9920373.1"
                     /translation="MEKMVAAMEMKKEILMTKKHLLFGSILLGILSFSPMLLSVANKG
                     NKKGELKLNPNYYNSQNEEVFVEKLDK"
     gene            complement(4763..5296)
                     /locus_tag="IWM35_002555"
     CDS             complement(4763..5296)
                     /locus_tag="IWM35_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HAP9920374.1"
                     /translation="MQPQETFTIKELSDLFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLDNQRLLDKLHKHEQNNDKNAYIPNKNVVANKLLDQLREENEFLRE
                     QLALKEQNNSHIQKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENSSTTLSEATE
                     TMDEQTKNWKKWWQLWK"
     gene            complement(5317..6159)
                     /locus_tag="IWM35_002556"
     CDS             complement(5317..6159)
                     /locus_tag="IWM35_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HAP9920375.1"
                     /translation="MREKGDKTVRFTFDQLKDLSNYKATANVRFVEDLKATYEKILSL
                     RFGRRSESGLSYDMFVMFTEFKINGDVENPYVDIRIYEKALPLLNNLDEWVRYSLQQF
                     NELQSGYAKTMFRLLKQYRTKGFAYFSKEDFLELLDIPKSYKQPDIDKRVIKPIRQEL
                     TAIFKGLTIKKKYGKGRGKPVIGYQFTFKPEIKKADDFNKHAPTKEDQNKALALADKQ
                     KEYEEPATTINEKIKQKKRELELLQQIKQLEKEKKALEKQTESALSEKEKKSLLSDLD
                     NLFQ"
BASE COUNT         1633 a         1151 c         1042 g         2347 t
ORIGIN      
        1 aaaaaaacaa attcatttca accggtgtaa attttcttaa aggaattgta tttaattcat
       61 ggtggtattt tacaatctca ttagccatat aatcacctca atccaattat gaatgacatt
      121 ttttaaatgt caagacattc atatttgacg ttcaaacaga caaaatgtcc ttcataaaca
      181 gacaaaatgt ccttcataaa cagacaaaat gtccttcata aacagacaaa atgtccttca
      241 taacattctg tatccattgg tagagtaagg acacagctta cggtaaagag gtttaaaaag
      301 aggtttaaaa agagatataa aaagaggggg gctaattttc ccgcctaaaa accacttctt
      361 ttttctttaa acaggttcgc gcatttttca aaactgccaa tccaaaacct tgcttcctac
      421 ggtcgccaaa aaggtgtgat cgctacgctc tcacattgta ctgcgcagtc gcctaaaggc
      481 tcctttgcta aaaacatgtt ggtaaagagg cctttgagcg gttctgaatg cttatctaag
      541 ccgttttaag acagtcttgg ataaaaatcc ttatctagct ttaaagcgcc ttacaggcgg
      601 tcagggcaag ccctaacaac cctaaaataa gcgatactta aaaacaccac acaagtttaa
      661 ttttttaaga tataataact tgtcgcctat ccgacagtct tgcgactgga aggaggtgta
      721 tctaatgttt gaattgttca tttgccccct tcttatagaa gtttgtgtct ctttattcag
      781 ttattggttg aataaccgaa acaagaagta aaatcaataa aggcgacatc aacctgaaaa
      841 aaagccccag agaatccgac ctctctgggg ctttcgtgta tctaatgttt gatcatttgc
      901 atgtttagtt taacatgatt gccgatcaaa ttcaattttg ctgttgctga tctaaccatt
      961 ccttgacact ttttagataa tttaatgccg tttctttttt cgttggattt tgtatgttcg
     1021 ttgcttgctg aagcagtgct ttataacgtt cgtaggtaac tgattgttgc cgttcttttc
     1081 gttcctgttc actagttgaa aaagggattg tatcactgaa taaattataa caagcttctt
     1141 ctgtctgcgt atctttaaaa tattgcacgt cttttttggt tcgctcatag ttgtcttgaa
     1201 cctctttttg ctgtttttct ggcaagtttt gataaagacg agtgagtttt tccatgtttt
     1261 tatccgtcaa acgtttttct tgattcacaa aaatatcgtt catttcctca taacaagtgg
     1321 taaactcttc cattttttgt gttaacactt gctccttttt ctttttatca catgctgctt
     1381 catacttttg agtgatatcc cctaataaaa tacttcttga aatacgttta tttccctcta
     1441 aaacgccgaa taaaccactt aaaacgattg ataatgtaaa tataacccct ggcaataaaa
     1501 acctcttaaa tcgaatttta gagcctttta atgaatatct tgtttgataa gccttatagc
     1561 taagccaaat aattcctaaa atactggcaa aaatgagtag atagggtaaa tagaatgcca
     1621 tttctattta ttctccccat accagccaaa aggctaacag tgtcatgatt aagcaacata
     1681 agggggcaag aatatccatt gatcgctgca taattacttt tttctgttct ttatttcgct
     1741 ggtttaatag cgcatggtgg aattttttga cttgtgaatc aattgtagtt tgtattcttt
     1801 ctttggtata aggtgcaaga aaagcacgtt gtttctttat ttcattttct atgtcttgta
     1861 acgttttaga acgataattc ttgttggtag tctgctcctc tttcaacgtg ttggtcatgg
     1921 tttcttcgag tcggtctaat ttccggttgt tcagtcgtaa gagttcgttg ttctcttcgt
     1981 acttcttttg cagtttcgcg aatgctagct cgagctcttc gtagctcgtt ggttcgttgt
     2041 tgattgtagg ctcgttctcg tctgtccagc tcatgtaaaa tcgtctcctt ttcgtaatcc
     2101 tttcctagtt tgttggatcg tacttttttc tttttgtgga tcaattcgta agtaaaattt
     2161 ttcccacgat tcaagcaatg aatgccgaat gttttaagag aatcacaaaa ttggttatac
     2221 gttcgagttt ttttgttttt cattgtttga tcaatcgcaa accgaatttc gtctttccaa
     2281 acaggttcat ttttttcttt taatttgatt tcagacatgg ttcgtttttc gggtttcttc
     2341 cctcgttcag tgattgttaa gtcatgttca agacaaatct cgtcatttag gtcataacat
     2401 ttgtttaaaa atgcttgacc atgggcttga aatttgttcc cagtttccag attgacggag
     2461 ttaatgacaa tgtggttatg aatatggtct ttgtctgtat gtgtgtaaat cgcgatttgg
     2521 tggtcagggg ctattttttt cgcatactct gcccctagtt gattggcttt ttcagcggta
     2581 atttctcctg gtcgaaaaga ctggatcaat aagtgtgctt gtacacgatc gtcttttcca
     2641 tacagcatgc gaatttgttt catttcgctt ttggcataat caatgtcaca gtttatccct
     2701 gttttgacgg ttgctcgtgg ttcggcgtaa ttgatcgctc ggctacaaga agtggttcgg
     2761 cttactttaa ctgttgccat atcttttgta gctccttttg catgattttt aggttttcgg
     2821 ataagcgttg ggcgtcgcca ggatcaacgt cgtgttggtg tgtgttgcac cacttggcaa
     2881 tttgattgat attcccgcca gctttggcta aatcgcgcgc gattgcctga gcaatgtctg
     2941 gcgccacttt cggtgtcacc agacgcgcgt tttgtgcctt ggctttcacg aaagccggca
     3001 cactcatttg caaagcttca gctgactgct tcagctttgc aaattcggat tcgctcactc
     3061 gaaaactgat ttgttttggc tcggatcgat aggtatattt tttagaatga ttgctagcca
     3121 aatattgatt ttgttcgctc ataagtccca ccctttcagt acgtcctacg gacgtatcgt
     3181 tcgcaatacc attgtaccta aaaaggtaca ataagtaaag ctcactaaag ctcactaaag
     3241 ggcttggcaa gctctatatt tgctagccaa atacctacgt atttggtgca aataaccgct
     3301 tgtcaaaggg gcgcaagctc ccccttgaat ccccctcccc cccactctcc acaatacaaa
     3361 aaagactagc aaatgctagc cactttttgt aaagtttcgc ttgtttcacc ttcggtgaaa
     3421 ttgttgcaaa tcaataatta ggtagagcta catttttttc gttgttcaaa gattcttatg
     3481 ccatttacta ttaataaatc tataaatatg ataatttttc ggctctataa tttatgggac
     3541 tgatgaatca gaattgattc ttcagtccca ttttcatttt attccaaaga attaactcca
     3601 aataccaata gaagcccatc ctctatatac ttgagattcc ttactaacaa tttcttgtaa
     3661 ttctattaaa gatttaggta atccaaattt atgttttaaa atatatcttc caagaaaact
     3721 acttaatttc atactaactt caccaacact ttttccctca ctgatttctt tagaataaag
     3781 aaagagcttt tcttttaagt caggatagtc atctatagtt gcttcattgt atactttact
     3841 aattgcattt aaaagttttt cttctttagt aaaatccatt ataataacct ccctaccact
     3901 accttctagg tgcccatgga ccatgattaa cccaaccatt aacaataatt tttccaattt
     3961 ctcctttagc ttgattccaa tctacccaac atttttcttt gttacaataa agaccatttc
     4021 catagtaagt agctgcatca acttttattc ctgtaccgat gccagctaga caagttccta
     4081 atattcctag aataacacaa tgttttaata ctttcttttt catgattgtt tttccttttc
     4141 gtttttttaa aattgcttgt tatattaaat aatcttagta attttattat ataacatttt
     4201 attatataac aatgattaac gtcataaaaa gtagttttga attcataatc gtttaaataa
     4261 aattataaga tcagttattt aaacagctag tagacataat gtgtatacta ccaagtaacg
     4321 cctgtaccaa gggatccagg cgctgatttt gtgaattttt attggtctag gttaaaagct
     4381 ctttttaagg cttggttttc tgcggttgtt tctttctaat tctttgtgaa tattgatcgg
     4441 tcatttttta aatacttgtt atttttgttt gtgtttgtta tgctttttac atatctcata
     4501 ttcatttatt attcttttct aaggttaata ataggataat ctctttcaga aagactgctc
     4561 gtacgagcag tcttttctta tttatcaagt ttttctacaa atacttcctc attttgactg
     4621 ttgtagtaat taggattgag ttttagttcc ccttttttat ttcctttgtt ggccactgat
     4681 aataacattg ggctaaagct taatattcct agtaggatag aaccgaatag caagtgtttt
     4741 tttgtcataa gtatttcctt cttcatttcc atagctgcca ccattttttc caattctttg
     4801 tttgttcgtc cattgtttct gttgcttcag aaagagtcgt tgaactattc tctgaagact
     4861 cttccgtata agtcaactgc agttgctcct gaagtttttc gatttgttgg ttggcttgca
     4921 gggtgagttg ttgttgctgg tcgagtagtt tttgtatatg agaattattt tgctctttga
     4981 gggcaagttg ttctcgtaaa aattcatttt cttcacgcaa ctgatctagc aacttgtttg
     5041 caaccacgtt tttgtttggt atataagcat ttttatcgtt gttttgttca tgtttatgca
     5101 acttatctag caacctttga ttgtctaagt tgcgagcaag ttgcaatact ccagaacgca
     5161 atacaacctt ataaccacgc tcatttttag ctatcatgga agaatctaat ttttggatat
     5221 gtttcgatat tgcttgccta ctcatcttaa aaagatctga caattccttt attgtaaagg
     5281 tttcttgtgg ttgcatactg gttgcctcct aggttgctac tggaataaat tatctaaatc
     5341 agataataaa ctttttttct ctttttccga aagtgcggac tctgtttgtt tttcaagagc
     5401 ttttttctct ttttctagtt gtttgatttg ttggaggagt tcaagttctc gtttcttctg
     5461 tttgattttt tcgttaattg ttgtagcggg ttcttcgtat tctttttgtt tatcagctaa
     5521 agccaatgct ttattttggt cttctttggt tggtgcatgt ttgttaaagt cgtctgcttt
     5581 tttgatctca ggtttgaacg taaattgata gccaataaca ggttttcccc gtccttttcc
     5641 gtatttcttt ttgattgtta gtcctttgaa gattgcagtt agttcttgac gtataggttt
     5701 tataactcgt ttatctatat caggttgctt ataactttta ggaatatcta ataattcaag
     5761 aaagtcttct ttggagaaat atgcgaaacc tttggttctg tattgcttta acagacgaaa
     5821 catagtcttt gcatagccac tttgtagctc gttgaattgt tgtaaagaat agcgtaccca
     5881 ctcgtctaaa ttatttaaca aaggcagtgc tttttcgtaa attcgtatat ctacgtaagg
     5941 attttctacg tccccgttga ttttaaattc tgtaaacatt acaaacatat cgtaagaaag
     6001 cccagactca cttctacgac caaagcgtaa agataagatt ttttcataag ttgcttttaa
     6061 atcttctaca aatcgtacat ttgcagttgc tttgtagtta cttaaatctt ttagttggtc
     6121 aaacgtaaaa cgaactgtct tatccccttt ttcacgcatt ctagacacga tgg
//
LOCUS       DACDXO010000097         6133 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E23605_2017
            SAMN12639989-rid9881463.denovo.101, whole genome shotgun sequence.
ACCESSION   DACDXO010000097 DACDXO010000000
VERSION     DACDXO010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639989
            Sequence Read Archive: SRR10023389
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6133)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6133)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741914.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:42:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,972
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 33 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 17 of 73
            Pseudo Genes (multiple problems)  :: 10 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6133
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639989-rid9881463.denovo.101"
                     /isolate="E23605_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(207..1169)
                     /locus_tag="IWM35_002557"
     CDS             complement(207..1169)
                     /locus_tag="IWM35_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018375965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP9920376.1"
                     /translation="MVAKLTDVAELAGVSPTTVSRVINNKGYLSEKTKQKVHEAMKTL
                     GYKPNNLARGLQGKSPQLIGLIFPNISNIFYSELIEYLEIELFKHGYKTIICNSQNDP
                     AKEREYIEMLEANQVDGIISSSHNLGIADYERVRAPIVAFDRNLAPNVSIVSSDNFEG
                     GKLAAKTLQKNGCQNTIMITGNDNTDSPTELRALGFSFQNPNGKIFKIPNNLSTIRRE
                     MEIKSTIINHKPDGIFVSDDLTAILTMKIAHQLKLNIPEDLKIIGYDGTSFIEKFFPQ
                     LTTIRQPIDEIASLIVDILLKKIKGEKTNKDYILPISILSGGSI"
     gene            complement(1171..2610)
                     /locus_tag="IWM35_002558"
     CDS             complement(1171..2610)
                     /locus_tag="IWM35_002558"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HAP9920377.1"
                     /translation="MNLPREIRYRAYSDWTKDEMDKIKDNVKRSPWRASYHIEPKTGL
                     LNDPNGFSFFNGKYTLFYQNWPFGAAHGLKEWVHNESDDLVHFHVTGAELLPDTKRDS
                     HGAYSGSAYEIEGNLFLLYTGNVRNENWERDTLQIGAWMDKDYNITKGENVLIHKPAD
                     VTDHFRDPQMFNYEGQLYAIVGAQNLEKSGFIKLYKADDNNVETWEEVGNLDFGGTGS
                     EYMIECPNLVFVDKKPVLIYCPQGLDKSELNYGNIYPNTFKICQSLDIEKVKLVGASE
                     IQNLDYGFEAYATQGFNTPDGRALIVSWIGLPDIDYPTDKYDYQGAMSLVKELSIKDG
                     KLYQYPVEAITSLRASSEQFTAKAETNNTYELELTFPPNQKSELLLFSDSKGNGLSLI
                     VDTKEGKIILDRSKAGIQYATDFGTIRECSIDTKETTANIFVDNSIIEIFINKGEKVF
                     TSRVFPENHQNGIQIKSGEPTGKYFELKY"
     gene            2795..4741
                     /locus_tag="IWM35_002559"
     CDS             2795..4741
                     /locus_tag="IWM35_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIBCA"
                     /protein_id="HAP9920378.1"
                     /translation="MNYSKVASDVIQAVGKDNLIAAAHCATRLRLVLKDDTKVNQKAL
                     DENPDVKSTFKINGQYQVIIGAGDVNFVYDELIKKTGLSEISTDDLKQIASKDKKFNP
                     IMALIKLLSDIFVPIIPALVAGGLLMALQNFLTAAGLFGPKSIEEMYPAIEGISSMIQ
                     LMSAAPFMFLPILVGISAAKRFGANQFLGAAIGMIMTTPDLGGSTEYWNIFGYHVSQT
                     NYAYQVIPVLAAVWILSILEKFFHKKLPSSIDFTFTPLLSVMITGFLTFTVIGPVMLL
                     LSNGITDIIVWLYNTTGFIGMGIFGGTYSLIVMTGLHQSFPAIETQLLSAWTNGIGHG
                     DFIFVVASMANVAQGAATFAIWFLTKNSKTKSLASSAGVSALLGITEPALFGVNLKYR
                     FPFFCALIGSGIATAVAGLLKVVAVSLGSAGFLGFLSINATSIPFYVLCELISFVVAF
                     AITYFYGKTKAAGIFKAEEIAEQTPVNSSKVATTDPISTDVESTQTKVSSETIVSPIS
                     GKVISLSSVNDPVFSSEAMGKGIAIKPNGNTVHSPVDGTIQLTFETGHAYGIKSNNGA
                     EVLIHIGIDTVSMGGKGFTQKVTTNQKVRKGDILGAFDRSMIEKEGLDDTTMIIVTNT
                     SDYSEVNIVAENTVTEGTDLLEIK"
     gene            5005..5877
                     /locus_tag="IWM35_002560"
     CDS             5005..5877
                     /locus_tag="IWM35_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP9920379.1"
                     /translation="MLYGAIEAGGTKFVCAIGDEEMTIKERVSFPTTTPEETMPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPKLAWQNFDFIGTMKKEFPIPIS
                     WTTDVNAAAYGEYVFGSGKGLSSVVYYTIGTGVGGGALQEGRFIEGFSHPEMGHMLVV
                     PHPKDDFAGSCPFHGNCLEGMAAGPAIEKRLGKKGQEVAEDDPYWEIEASYIAQCAYN
                     TTLMFSPDVIIFGGGVMKQRHMLQNVHDAFAKLVNGYVETPELSKYIVTPALEDNAGT
                     LGCLALAREAVLHS"
BASE COUNT         1954 a         1056 c         1080 g         2043 t
ORIGIN      
        1 cgtccaattt cgtaaaacaa ccgtaacagt tgtgtaaaaa tatcatttta gagcatgaaa
       61 aaaggagtgg atttctccac tccaaaaaag tctaactttt gggggtcact acaagttgac
      121 aactcctttt tgtttacaaa tcaattggat atctacaaat tgtattttct aacgaatttt
      181 atagacatta aaaaattaat ttaacgttaa atacttcctc cagaaagtat tgagattggt
      241 aaaatataat ccttatttgt cttttcgcct ttaatttttt tcagaagaat atcaacaata
      301 agacttgcaa tttcatcaat tggttgtctt attgttgtca attgtgggaa aaatttctca
      361 ataaaagagg taccatcgta tccaataatt tttaaatctt ctgggatatt aagtttaagt
      421 tgatgagcaa ttttcatagt gagaatggct gttaaatcat cagaaacaaa aataccatct
      481 ggtttgtgat ttataatagt tgactttatt tccatttcac gacggatagt agacagatta
      541 ttaggaattt tgaaaatttt tccatttgga ttttgaaaag aaaaacctag tgcacgtaat
      601 tctgtgggag aatccgtgtt atcatttcct gtaatcataa tggtattttg acaaccattt
      661 ttttgtaaag tcttagctgc aagttttccc ccttcaaagt tatctgaaga aacaatggaa
      721 acattaggag ctaaatttct atcaaaagca acaataggtg ctctcacacg ctcataatca
      781 gcaattccaa gattatggct tgatgagatt atgccatcaa cttggttggc ttcaagcatt
      841 tcaatgtatt ctctctcttt agcaggatca ttttgactat tgcaaataat cgttttatat
      901 ccatgtttaa ataactctat ttcgagatac tcaatgagtt cagagtaaaa gatattacta
      961 atattaggaa aaataagacc aataagctga ggtgactttc cttgaagtcc acgtgctaaa
     1021 ttatttggtt tataaccaag agttttcata gcttcatgaa ctttttgttt tgttttttct
     1081 gataaatatc ccttattatt aatgacgcgt gaaacggttg ttgggctaac tccagctaat
     1141 tcagcgacat cagttaattt tgctaccatt ttaatatttt aattcaaagt attttccagt
     1201 aggttcgcct gatttgattt gaataccatt ttggtgattt tcagggaata cacgactagt
     1261 aaacactttt tctcctttat taataaaaat ctcaatgatt gagttatcta caaaaatgtt
     1321 agcagttgtt tctttagtat caattgagca ttcacgaatc gtaccaaagt cagtggcata
     1381 ttgaatgcct gctttgctgc gatctaaaat aatttttcct tctttggtat ctacaatcaa
     1441 gctaagtcca tttcctttac tatcagagaa aagaagtaac tccgatttct gatttggagg
     1501 aaaagttagt tcaagctcat aagtattatt tgtttctgct tttgctgtaa attgttcaga
     1561 actagcacgt aatgatgtaa tagcttctac tggatattgg taaagtttac catcttttat
     1621 tgaaagttct tttacaagac tcattgctcc ctgataatca tacttatcag ttggataatc
     1681 gatatcaggt agtccaatcc aactgacaat cagagcacga ccatcaggag tattgaatcc
     1741 ttgagtcgca tacgcttcaa atccataatc aagattttga atttctgaag cgcccacaag
     1801 ttttactttt tctatgtcaa gcgattgaca gatcttaaaa gtattcggat agatattccc
     1861 ataatttagc tcagatttat ccaacccttg aggacaataa atgagaacag gttttttgtc
     1921 tacaaatact agatttggac actcaatcat gtattctgaa ccagtgccgc caaaatcaag
     1981 gttgccaact tcctcccatg tttccacgtt attgtcatcc gctttgtaaa gtttgataaa
     2041 gcctgatttt tctaaatttt gtgctccaac aatcgcatac aattgtcctt cataattaaa
     2101 catttgtgga tcacgaaaat gatcagttac atcagcaggt ttatgaataa gaacattctc
     2161 acctttcgta atgttgtagt ctttatccat ccatgctccg atttgaagtg tatcacgttc
     2221 ccaattttca tttcgaacgt ttcctgtata gagtaagaat agattcccct ctatttcata
     2281 tgcagaaccg gaataagcgc cgtggctatc tcgttttgtg tctggaagaa gttctgctcc
     2341 tgttacatga aaatgaacta aatcatctga ttcattgtgt acccattctt tcaacccatg
     2401 agcagctcca aatggccaat tttgataaaa aagcgtatat tttccgttaa aaaaagaaaa
     2461 accattagga tcgtttaaca atcctgtttt aggttcgata tgatagctag cacgccaagg
     2521 tgatcgtttt acattatcct taattttatc catctcatct ttagtccaat cagaataagc
     2581 acgatatcgt atttctcgag gtaagttcat attttactcc ttcaatttat ctttttacaa
     2641 ctagtatggt aaacgttttc cgttgaaaaa tcaatcattt catttaaaaa aataaactat
     2701 atgacaaacg tttgacatat ttttaagata tgctaaaata aatttgcatt tcagtgaacc
     2761 gttttcacgt aaaaataaaa aaggagtgat tcgaatgaat tacagtaaag tagcttccga
     2821 cgtaatacaa gcggtcggaa aagataacct tattgcagct gcacactgcg ctacacgtct
     2881 tcgtctcgta ctaaaagatg acacgaaagt gaatcaaaaa gctttagacg aaaatccaga
     2941 tgtgaaaagc acatttaaaa ttaatggaca atatcaagtg attatcggtg caggtgatgt
     3001 taatttcgtt tatgacgaac ttatcaaaaa aactggactt tcggaaattt caacagatga
     3061 tttaaaacaa attgctagta aagataaaaa attcaacccc attatggcat taattaaatt
     3121 attatcagat atttttgttc caattatacc tgcccttgtt gcaggtggtt tattaatggc
     3181 attacaaaac tttttaacag ctgctggact ttttggacca aaatcaattg aagaaatgta
     3241 tcctgcgatt gaaggtattt cctcaatgat ccaactgatg tccgctgcac cattcatgtt
     3301 cttaccaatt ttagtaggta tttcagctgc taaacgtttt ggagcaaacc aatttcttgg
     3361 tgctgcaatt ggtatgatca tgacaacacc agatcttggt ggctcaactg aatattggaa
     3421 tatttttggt tatcatgttt cacaaacaaa ctatgcttat caagttattc cagttttagc
     3481 agctgtttgg atattatcta tattagaaaa attctttcat aaaaaattgc catcatctat
     3541 tgactttaca ttcacgccat tattatcagt aatgattact ggatttttga ctttcacagt
     3601 tattggccct gtaatgttat tgttgtctaa tggaattaca gacattattg tttggctata
     3661 taacactact ggatttattg gaatgggtat ttttggagga acatattcac ttatagtcat
     3721 gactggttta catcaatcat ttccagctat tgaaactcaa ttactttcag cgtggacaaa
     3781 tggtatcgga catggagact ttatctttgt agttgcatct atggcaaacg ttgctcaagg
     3841 tgctgcaaca tttgccattt ggttcttaac taaaaattct aagacaaaaa gtcttgcttc
     3901 ctctgcgggt gtctcagcac ttcttggaat tacagagcct gccttgttcg gagttaactt
     3961 aaaataccgc tttccattct tttgtgcttt aattggttca ggaattgcta cagcagtagc
     4021 aggattactt aaagtagttg ctgtttcact tggttcagca ggtttcttgg gatttctttc
     4081 aattaatgca acatctattc cattttacgt attgtgtgaa ttgattagct ttgtagttgc
     4141 ttttgcaatt acttacttct acggtaaaac aaaagctgct ggtatcttta aagcagaaga
     4201 aattgctgaa caaacaccag ttaattcttc aaaagtagca acaacagatc caatatctac
     4261 tgatgttgaa tcaacacaaa caaaagtttc atcagaaaca attgttagtc ccatttctgg
     4321 taaagtgatt agtctttctt cagtaaatga tccagtcttt tcatcagaag ctatgggaaa
     4381 agggattgcc attaaaccaa atggaaacac ggtccattct ccagtagatg gtactattca
     4441 actcacgttt gaaactggac atgcttatgg tattaaatca aataatggtg ctgaagttct
     4501 aattcatatt ggtattgata ctgtatcaat gggaggaaaa ggatttacac aaaaagttac
     4561 tactaatcaa aaagttagaa aaggcgatat tcttggagca tttgaccgtt caatgattga
     4621 gaaagaaggt cttgatgata caacaatgat tattgtgacc aatacttcgg attattcaga
     4681 agtaaatata gtagctgaaa atacagtcac tgagggtact gatttacttg aaatcaaata
     4741 aatatatttc cctgagtaaa gatggaattt tctttccaaa atcaaataat atagattaat
     4801 caaatatttg agaataacat tttatttaat cttttcatat atcaattgta tgaaaaatta
     4861 ttagaatttt aaaaaatgat aaaaattcaa aaagaaaaaa tagtagttat ttacttgtca
     4921 tgttactaaa taaaaaaata atttaaatat gatatatagt catttattta gtgatttcat
     4981 tctaaaataa agggagaaat gtctatgcta tacggtgcaa ttgaagcagg cggcacaaaa
     5041 tttgtgtgtg ccattggcga tgaagaaatg acgatcaagg aaagagtgag ctttccgaca
     5101 acgacacctg aggaaacaat gcctttagtc attgacttct tcaaacaata ccaagcagat
     5161 ttagcaggga ttgggattgg ctcatttggg cctatcgata tccatcgcga ttccgccact
     5221 tatgggtaca ttacctcgac cccgaaattg gcatggcaaa attttgattt catcggtacc
     5281 atgaaaaaag aatttccgat cccgatttct tggacaacag atgtcaatgc ggctgcctat
     5341 ggtgagtatg tcttcggcag tggcaaagga ttgagcagtg tcgtttacta tacgatcggt
     5401 acgggtgttg gcggcggtgc cttacaagaa gggcgcttta tcgaagggtt tagtcatcca
     5461 gaaatggggc atatgctcgt cgtccctcat ccaaaagatg actttgctgg cagctgtcca
     5521 tttcatggca actgtttaga agggatggcc gctggacccg ccatcgaaaa acgcttaggg
     5581 aaaaaaggac aagaggttgc agaagatgat ccttattggg aaatcgaagc ctcctatatt
     5641 gctcaatgtg cctacaatac aaccttgatg ttttctccag atgtgatcat ttttggcggt
     5701 ggtgtgatga aacaacgcca tatgctgcaa aacgtccacg acgcatttgc aaaattggtc
     5761 aatgggtatg tcgaaacacc tgagttaagc aaatatatcg tgaccccagc attagaagac
     5821 aacgctggaa cgctcggttg tttggcattg gctcgagaag cggtcttaca ttcataaacg
     5881 aaaaaaggct cttcgacatt tagtgttggt agagaaagat acaactgaaa atttagtcat
     5941 aaccagttag gcagagtgct taactggttt ttgttttact tggaagatca agccttgaat
     6001 gagataaatt cttgctcgaa agttatggaa atttcgatag ccgtaggcca cttgtattgt
     6061 gcaatagttt tttacacgcc gtttgtataa ttaagcgaga caaataaaaa agccatttat
     6121 gttacactca aat
//
LOCUS       DACDXO010000098         6093 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E23605_2017
            SAMN12639989-rid9881463.denovo.102, whole genome shotgun sequence.
ACCESSION   DACDXO010000098 DACDXO010000000
VERSION     DACDXO010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639989
            Sequence Read Archive: SRR10023389
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6093)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6093)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741914.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:42:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,972
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 33 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 17 of 73
            Pseudo Genes (multiple problems)  :: 10 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6093
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639989-rid9881463.denovo.102"
                     /isolate="E23605_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            50..943
                     /locus_tag="IWM35_002561"
     CDS             50..943
                     /locus_tag="IWM35_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HAP9920380.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEMAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            1077..1202
                     /locus_tag="IWM35_002562"
     CDS             1077..1202
                     /locus_tag="IWM35_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9920381.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(1336..1968)
                     /locus_tag="IWM35_002563"
     CDS             complement(1336..1968)
                     /locus_tag="IWM35_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9920382.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
     gene            complement(2116..2658)
                     /locus_tag="IWM35_002564"
     CDS             complement(2116..2658)
                     /locus_tag="IWM35_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HAP9920383.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            2793..3182
                     /locus_tag="IWM35_002565"
     CDS             2793..3182
                     /locus_tag="IWM35_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9920384.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            3186..3950
                     /locus_tag="IWM35_002566"
     CDS             3186..3950
                     /locus_tag="IWM35_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAP9920385.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIPLMEEASTFLAKNLIKKYK"
     gene            4079..5026
                     /locus_tag="IWM35_002567"
     CDS             4079..5026
                     /locus_tag="IWM35_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP9920386.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
     gene            5156..5896
                     /locus_tag="IWM35_002568"
     CDS             5156..5896
                     /locus_tag="IWM35_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9920387.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
BASE COUNT         2144 a          987 c         1192 g         1770 t
ORIGIN      
        1 acgtaaaagc cttttaagta aaataaaacg gatagaaaag agaacatata tgaactatta
       61 ttcaaaaaat aaggaaaact tttatcaaaa actgaccgat gatccgcttt tttcttcatt
      121 gaccgattat ctatatgaac atcgagaaca agaaacgatt ctaagagaat tgaaaaaaga
      181 attttcgcaa aataaatttt cccattttct tgatttattg atcgatgcag gattaatcaa
      241 gagggaagaa cgaagatatc atttgaactt tccaatattt gatcctaaag attacttgca
      301 acaagccact tcggctgctg aaaccatagc tgaacaattg aagagactct ctgttgatga
      361 acaaaaatta gcgatgggag aagttatttg ggcgtattgt ttcgaagatg aacggaaaga
      421 agcgtatttt tatggagtaa gaaacagccg agagacagaa ttgctgagga caactgcagg
      481 aaatgagaag taccgtttta tcacactttc ctctatagaa cacttcccgc ttactttggc
      541 taattacttt tttgtacaga agaatcagct tcctgtaacg aaggcattca aagaattagc
      601 cgaacttatt ggagacgtaa atgaatcgta cttttttgat caaatagaag ttatcgttga
      661 caggatacgt aagaataaat ataaaaatcg ccggcctagt attttccatc agtctctact
      721 tgtgacgaat actataaagg aagaagaatc attcacatta gaattaccta ttgtcgaaaa
      781 aaacaatttt gagattgaat taccaacatt ggatccttca ttgacaatgg aggaaatggc
      841 ctttcttaaa cgacaaatct tttctgagct gagcaagaaa tttataccgc atgcattttc
      901 ttatatcaaa gaatatggaa cagttttagt gagtaaaacc taagagagca gtaagcaaaa
      961 attatactga ctcttgcagg aggagccttt ctcttccttt atggttaaaa gaattatttg
     1021 agtattttgc ctatctacca cctattagat tcactacatg gtctggaaac aaaggaatgc
     1081 aaaaaaatgg tgagaaatgt ggaatgacaa aggaagtagt gataagaaaa gttagatttt
     1141 taaataatca atattatgat tcagtcaagt atggcatttt atgggaagaa ctagctgatt
     1201 agattaggaa gtccatttga taatccagaa tcactaagaa acgaatggcg agtcattaat
     1261 aatttatcca atcttgggtt attcttttag attgaagtgt ttaaaactaa aaaagcattt
     1321 atgaagtcgt aatagtcagg cttcaaaatg ctttttattt tgtttgtttt ttctttgcca
     1381 cataaataca cctagactga cgatgaagag catatccaac agatttcctg cgacttggat
     1441 cgtcgaaggg ttggtcagtg gcattaaagc aaggacaaaa aaggaaaaca agctgtaaat
     1501 aaaacaggtg acaggaacta atcgaaagaa gagcggacat ttcaattggc taaaaatgag
     1561 tgaaaaaata aaataagtaa aaatggataa aatcacgact aaaccattca tgataaagaa
     1621 gaaaagcgta ttgtgattaa acgaataaaa aagaattgct aaaaaagaaa acgttttaaa
     1681 tgcaatcaat acgcttgaag cttcgtttaa cgtaccagga tgtcgatgaa aaaaataggc
     1741 accgaaataa acagaaaaca gtaagccgca aagagaagac aaaatcaaaa aaaacggatt
     1801 ttcttcggct tctgtgatat cagaatgatc caaaagcaac cagcctaatg cactagaagg
     1861 cgcatgtgag gtcttaaggt atgaataaca tagaaaaaat aagatcgtca aagttgcaaa
     1921 aaaagagcct aaatgtaaaa aattagtggt tctacgttga atttccataa tatctccaaa
     1981 ttaaattgat aagtatataa agtataataa caaattgatg agtatatgtc gatgtttttt
     2041 aatcaaaata ataaataaaa actgtctaac atttaaaatt taatttagat attagacagt
     2101 ttttaagttt gtgagttatt ttccttgtcg ttttctcact tttttagtcc agaaaccacc
     2161 tgaaatatat tttggctcat aagaaatgat aaaagcacgc tcttcgattt ctttgatcag
     2221 gcgatacagt tcacgttcgc tttttctagg agaaagtatt tcgagaacta gccgctcacc
     2281 ttccagcccc attgcagaag tctgtgtcac accataaccg tgttctctca gtatacccgg
     2341 caaactttta tcttctgcgc tatttgctgg caaaatcgtt gtgaccatga tgtatcctaa
     2401 tgccaatttg tcttcaatca aaattccgac actgatacct actgcgtaac ctaacgcata
     2461 gacaaataaa ttcagtggat tatctaaacg atctaaaaca agggataagc cgacgatata
     2521 aatcgtgatc tcgaccatac tgacaagtgg agcaatcagt cgatagcctt tcattgtcaa
     2581 catgaaacgt aacgtgttca gagtgatata agcaaaatta atgaggaaga tcatccccaa
     2641 catttttgga tcaatcattt gtaaaactcc tttcatttat ataacaacaa tatcaaaaaa
     2701 aatcgtaagt gaagaaaaat tgaaacttta tacatacaaa aaaatgataa aatgattata
     2761 atagaattat aaagataggt taggtgatta aaatgaaaac ggtcaatcgt gtgttttgtg
     2821 ccagccacgt gctaagtctc cttctcagta tatacttagg tgtctattcg attcaacagc
     2881 caattgaggt agagaacatt gtttttcaga tgtcgctgac agatggttta attttaagcg
     2941 tttttttctt tttgatactg tttattggta atattttcgg tagcgttgcc agttttttga
     3001 atttttctat ttatccactc ctttcgctag cattaggagt ggtgggactt tgttctctgt
     3061 gcttatttcc cgagccattc agtcctgcat ttatattatt tggtacactg aacctgttcc
     3121 agacaactgt tggagcatgg ttgctatggc gatcaggaaa tttaatgaaa ataggtgagt
     3181 aacaattgaa tccaattata ttgaaacgtt ctgtccatca tattccagtg ttagaagttg
     3241 ttctgaaaga attaagagag gaacgtattc ccgttgtcat ctattatcac ggatggcaga
     3301 caagtaagga gttagttttg acgcaaggaa gaaaaattgc gcagcagggc attcgtgtga
     3361 tcctgccaga tgcgatgaat catggggagc gcaaacagcc ggtttcttct attccatcct
     3421 atacattttg ggatagtatc tacggaaatc tgtttgaatt tgacacactt atcaagcatt
     3481 tagaaaaaag aggtcttcta catgatcagc ttgcagttgg cggggtttcg atggggggaa
     3541 tgacaacatg cgccttgttg gctaaacatc ctcatatagt agcgggcgtg tgtttgatgg
     3601 gttcccctgc acctttggaa tatggaaaag aaatcgtaag gcgcgcatcg gaatataatt
     3661 atgtcgtacc aaaagactat tttgatttga tttcatgggt taagagctat gatttgtcat
     3721 tgcagccgga aaagttagct ggacgtcctc tgttcttttg gcatggacaa gctgacgaaa
     3781 aaatccctta tatacaaacg gaacgatttg tagaaaaaaa tcggcataag tcttacggaa
     3841 aagaaattgt ttttcgttcc tcaaaaacac agcgacatat ggtacaaata ccactaatgg
     3901 aagaagcttc cactttttta gcaaaaaatt tgataaaaaa gtataaataa ttgaaacgga
     3961 aaatcactca tgacgataga ggattttccg ttttttattt tgggctataa taaactctgt
     4021 taggaaaatt tggtataatt gatagtaaga atgacaacat gttagaaagg aagagagcat
     4081 gcccacttta gcagatgttg caaaacgtgc caatgtttca aaaatgacgg tctctcgagt
     4141 catcaatcat ccggaacagg tgactgacga attgaaagag ttagttttct cagcaatggc
     4201 tgaactggat taccgtccaa atattgctgc aaaagcactt gtcacgaatc ggtcgcagat
     4261 catcaaatta tttatactgg aagaaatcga cacaactgag ccctattaca tgaatttgct
     4321 gatgggaatc gctaaacgga tcggtaaagc gcattattct ctccaattag ttacaaatga
     4381 tgccttcgac gtcggttctt gtgatggata catcatcaca ggtgtcagaa aaaaagactt
     4441 cgattggatc gaccgtttgg aaaaaccagt tgtcttgttt ggggaaaatt ctgaaggata
     4501 tgattatgta gacagcgata atgaatatgg tacagcaaaa gcaacgcaat atgccttaga
     4561 aagagattat caaacgatta tttatatcgg tatcgaagtg gacgagcctt ttgaaatttc
     4621 aagggaaact ggttatttat ctgtgatgaa ggaagttgat cttcctagta aaatcttgcg
     4681 gtttccaaat cattcgacag aagtagaaag atatatgaca gaaaactggg cagattttac
     4741 agataaaact tgttttgtat gtagttcaga tcggctagca ctaggagcag taagagggat
     4801 tataaatgct ggaggacact tgcccgaaga atttggtgtg attggatttg atggggtatt
     4861 tttagaccaa atcagttcac ctaaattgac aacgatcaag caagatatta tacgtatggg
     4921 tgaagtatgt ggagaaatgt tgttaaaaaa aatagaagaa aaaggggcga gtcaaggtta
     4981 tcgtcgtttt tttcctgaac tagttatcag agagacaacg aattaagtct tattactata
     5041 tatatattta tgaaattcat cgctattttc ttaaaataat cagcgcattg ctactgaaaa
     5101 caatgaatct gtgctattat tgagaaaagt tccatttttg agaaggatgg tttgaatgaa
     5161 agaaatcaga cgaaaacatt tccgttttcc tgcgtacgat gatgagttag gcgtaaaatt
     5221 aaccaacgac aagaagcgag tattatttga cggacaagaa gatcttttga ctgaacagaa
     5281 tcagtccccg tttgaacaga tgaaagaatt tcatttcacg aacacgaaca ccgagaaaat
     5341 ccgtcgcaag cgtcgtgcaa caaatggatt gaagtcagct ccgcgtaagg aaaatttgga
     5401 aaagcataaa ggaaaattac cggattatac cggccattac caatcagaaa tcacatcgac
     5461 aggacgaaaa aaactttttg gtgattcctc tcgttctgtc agacaaagtg aaacaaggac
     5521 agagtccaag catactacac gaaacgcatc tgttgacacg aaggtgcggg agcgttctta
     5581 ttttgttcct aaatatgtac ccgcatcgat tatcccagac cctagagaaa gcaaaatttc
     5641 tgaggatcat ttaatcgaat cgatgaaaaa aacggatgac tcttatctgt tgttcgatac
     5701 agaaccgacg ccgtatcaag aaaaaagaaa tggtgagccg tctgtacgaa cattcaatcg
     5761 aacaaaaacg ttagaggaaa caaagcagtc agaagaaaaa aaatcgaaaa aaagccatgg
     5821 tatcttagaa cgctcattac aaggattgat cgatgatcaa ggaaactctt tgggagaaaa
     5881 tagctatttt aaataagtga tatacaaatt tatattcggc tacagttgga aaggaaacgc
     5941 tgcggcgttt ccttatttta tactctatcc gaaagtttat atcgtatcct caaatttttt
     6001 tgagtttaag cgaatttttt ctcccaataa gtaagaaaat aatggcaaaa taaaaaaaca
     6061 aactattttc gcagatcgtt tgcaaaaata gtt
//
LOCUS       DACDXO010000099         5904 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E23605_2017
            SAMN12639989-rid9881463.denovo.104, whole genome shotgun sequence.
ACCESSION   DACDXO010000099 DACDXO010000000
VERSION     DACDXO010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639989
            Sequence Read Archive: SRR10023389
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5904)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5904)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741914.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:42:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,972
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 33 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 17 of 73
            Pseudo Genes (multiple problems)  :: 10 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5904
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639989-rid9881463.denovo.104"
                     /isolate="E23605_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..986)
                     /locus_tag="IWM35_002569"
     CDS             complement(<1..986)
                     /locus_tag="IWM35_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006701269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS1542 family transposase"
                     /protein_id="HAP9920388.1"
                     /translation="MTQVHFTLNNEEVQSIIEHSVKDDVSKNILTTVFNQLMENQRTE
                     YIQADDYERSESRQSQRNGYYERDFTTRVGTLELKVPRTRDGEFSPTVFERYQRNEKA
                     LLASMLEMYVSGVSTRKVSKIVEELCGKSVSKSFVSSLTEQLDPMVNEWQNRSLSGTN
                     YPYLMTDVLYIKVREDHRVLSKSCHIAIGITEGGDREIIGFMIQNEESDDTWSIFFEY
                     LKERGLKGTELIISDAHKGLVSAIRKSFTNASWQRCQVHFLRNIFSSIPKKNSKPFRE
                     AVKAIFKFTDIELARTAKNALVGEYIDQPKYTKACEILDNGFEDAFQYTVIG"
     gene            1138..1833
                     /gene="vanR"
                     /locus_tag="IWM35_002570"
     CDS             1138..1833
                     /gene="vanR"
                     /locus_tag="IWM35_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HAP9920389.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            1811..2965
                     /locus_tag="IWM35_002571"
     CDS             1811..2965
                     /locus_tag="IWM35_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HAP9920390.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
     gene            3180..4148
                     /locus_tag="IWM35_002572"
     CDS             3180..4148
                     /locus_tag="IWM35_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HAP9920391.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            4141..5172
                     /gene="vanA"
                     /locus_tag="IWM35_002573"
     CDS             4141..5172
                     /gene="vanA"
                     /locus_tag="IWM35_002573"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HAP9920392.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALVVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            5178..5786
                     /locus_tag="IWM35_002574"
     CDS             5178..5786
                     /locus_tag="IWM35_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HAP9920393.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPKRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
BASE COUNT         1782 a         1149 c         1335 g         1638 t
ORIGIN      
        1 tttccgataa ccgtgtattg aaaggcatct tcgaagccat tatccaatat ttcgcaggct
       61 tttgtatatt taggctggtc gatatattca ccgactaagg cattcttagc tgttcgagcg
      121 agttcaatat ccgtaaactt aaagatcgct tttactgctt ctctaaacgg ttttgaattc
      181 ttttttggaa tggaactgaa gatgtttctt aaaaaatgga cctggcatct ctgccaactt
      241 gcgttggtaa atgacttacg aatcgcagac actaggcctt tatgggcatc agaaatgatg
      301 agttctgtcc cctttaggcc gcgttctttt aagtattcaa agaagatgga ccatgtgtca
      361 tcactttctt cattttgaat catgaagcca atgatttcac ggtcgccacc ttctgttatc
      421 ccgatcgcaa tatggcagct tttagaaagc actcgatggt cctcacggac ttttatgtag
      481 agaacatcag tcatcagata aggataattc gtacctgaga gtgaacggtt ctgccattcg
      541 ttgaccatcg ggtctaactg ctcagtcagg ctagaaacaa aagatttcga tacagatttt
      601 ccacatagct cttcaacaat ctttgaaact ttacgtgtcg aaacgcccga aacatacatc
      661 tcaagcattg aagcgagcag tgccttttca tttcgctgat aacgctcaaa caccgtcggt
      721 gaaaattcac catcacgtgt tctaggcact tttaattcga gtgtacccac acgagtcgta
      781 aagtctcgct cataatagcc atttctttga ctctgacgac tttctgaacg ttcatagtca
      841 tcggcttgaa tatattctgt tcgttgattt tccatcaatt ggttgaaaac agtggttaaa
      901 atatttttag aaacatcatc ttttaccgaa tgttcaataa tactttgaac ctcttcgttg
      961 ttcagtgtaa aatgtacttg ggtcatgtaa cgtcctcctg ggtatgtttt tgtggttaaa
     1021 aacattgtac cgtaaaaggg ctgttacatg gccttttatc ttttacacaa ttatatggac
     1081 tttatcctat aatctgataa attattaagg gcaaacttat gtgaaagggt gataactatg
     1141 agcgataaaa tacttattgt ggatgatgaa catgaaattg ccgatttggt tgaattatac
     1201 ttaaaaaacg agaattatac ggttttcaaa tactataccg ccaaagaagc attggaatgt
     1261 atagacaagt ctgagattga ccttgccata ttggacatca tgcttcccgg cacaagcggc
     1321 cttactatct gtcaaaaaat aagggacaag cacacctatc cgattatcat gctgaccggg
     1381 aaagatacag aggtagataa aattacaggg ttaacaatcg gcgcggatga ttatataacg
     1441 aagccctttc gcccactgga gttaattgct cgggtaaagg cccagttgcg ccgatacaaa
     1501 aaattcagtg gagtaaagga gcagaacgaa aatgttatcg tccactccgg ccttgtcatt
     1561 aatgttaaca cccatgagtg ttatctgaac gagaagcagt tatcccttac tcccaccgag
     1621 ttttcaatac tgcgaatcct ctgtgaaaac aaggggaatg tggttagctc cgagctgcta
     1681 tttcatgaga tatggggcga cgaatatttc agcaagagca acaacaccat caccgtgcat
     1741 atccggcatt tgcgcgaaaa aatgaacgac accattgata atccgaaata tataaaaacg
     1801 gtatgggggg ttggttataa aattgaaaaa taaaaaaaac gactattcca aactagaacg
     1861 aaaactttac atgtatatcg ttgcaattgt tgtggtagca attgtattcg tgttgtatat
     1921 tcgttcaatg atccgaggga aacttgggga ttggatctta agtattttgg aaaacaaata
     1981 tgacttaaat cacctggacg cgatgaaatt atatcaatat tccatacgga acaatataga
     2041 tatctttatt tatgtggcga ttgtcattag tattcttatt ctatgtcgcg tcatgctttc
     2101 aaaattcgca aaatactttg acgagataaa taccggcatt gatgtactta ttcagaacga
     2161 agataaacaa attgagcttt ctgcggaaat ggatgttatg gaacaaaagc tcaacacatt
     2221 aaaacggact ctggaaaagc gagagcagga tgcaaagctg gccgaacaaa gaaaaaatga
     2281 cgttgttatg tacttggcgc acgatattaa aacgcccctt acatccatta tcggttattt
     2341 gagcctgctt gacgaggctc cagacatgcc ggtagatcaa aaggcaaagt atgtgcatat
     2401 cacgttggac aaagcgtatc gactcgaaca gctaatcgac gagttttttg agattacacg
     2461 gtataaccta caaacgataa cgctaacaaa aacgcacata gacctatact atatgctggt
     2521 gcagatgacc gatgaatttt atcctcagct ttccgcacat ggaaaacagg cggttattca
     2581 cgcccccgag gatctgaccg tgtccggcga ccctgataaa ctcgcgagag tctttaacaa
     2641 cattttgaaa aacgccgctg catacagtga ggataacagc atcattgaca ttaccgcggg
     2701 cctctccggg gatgtggtgt caatcgaatt caagaacact ggaagcatcc caaaagataa
     2761 gctagctgcc atatttgaaa agttctatag gctggacaat gctcgttctt ccgatacggg
     2821 tggcgcggga cttggattgg cgattgcaaa agaaattatt gttcagcatg gagggcagat
     2881 ttacgcggaa agcaatgata actatacgac gtttagggta gagcttccag cgatgccaga
     2941 cttggttgat aaaaggaggt cctaagagat gtatataatt ttttaggaaa atctcaaggt
     3001 tatctttact ttttcttagg aaattaacaa tttaatatta agaaacggct cgttcttaca
     3061 cggtagactt aataccgtaa gaacgagccg ttttcgttct tcagagaaag atttgacaag
     3121 attaccattg gcatccccgt tttatttggt gcctttcaca gaaagggttg gtcttaatta
     3181 tgaataacat cggcattact gtttatggat gtgagcagga tgaggcagat gcattccatg
     3241 ctctttcgcc tcgctttggc gttatggcaa cgataattaa cgccaacgtg tcggaatcca
     3301 acgccaaatc cgcgcctttc aatcaatgta tcagtgtggg acataaatca gagatttccg
     3361 cctctattct tcttgcgctg aagagagccg gtgtgaaata tatttctacc cgaagcatcg
     3421 gctgcaatca tatagataca actgctgcta agagaatggg catcactgtc gacaatgtgg
     3481 cgtactcgcc ggatagcgtt gccgattata ctatgatgct aattcttatg gcagtacgca
     3541 acgtaaaatc gattgtgcgc tctgtggaaa aacatgattt caggttggac agcgaccgtg
     3601 gcaaggtact cagcgacatg acagttggtg tggtgggaac gggccagata ggcaaagcgg
     3661 ttattgagcg gctgcgagga tttggatgta aagtgttggc ttatagtcgc agccgaagta
     3721 tagaggtaaa ctatgtaccg tttgatgagt tgctgcaaaa tagcgatatc gttacgcttc
     3781 atgtgccgct caatacggat acgcactata ttatcagcca cgaacaaata cagagaatga
     3841 agcaaggagc atttcttatc aatactgggc gcggtccact tgtagatacc tatgagttgg
     3901 ttaaagcatt agaaaacggg aaactgggcg gtgccgcatt ggatgtattg gaaggagagg
     3961 aagagttttt ctactctgat tgcacccaaa aaccaattga taatcaattt ttacttaaac
     4021 ttcaaagaat gcctaacgtg ataatcacac cgcatacggc ctattatacc gagcaagcgt
     4081 tgcgtgatac cgttgaaaaa accattaaaa actgtttgga ttttgaaagg agacaggagc
     4141 atgaatagaa taaaagttgc aatactgttt gggggttgct cagaggagca tgacgtatcg
     4201 gtaaaatctg caatagagat agccgctaac attaataaag aaaaatacga gccgttatac
     4261 attggaatta cgaaatctgg tgtatggaaa atgtgcgaaa aaccttgcgc ggaatgggaa
     4321 aacgacaatt gctattcagc tgtactctcg ccggataaaa aaatgcacgg attacttgtt
     4381 aaaaagaacc atgaatatga aatcaaccat gttgatgtag cattttcagc tttgcatggc
     4441 aagtcaggtg aagatggatc catacaaggt ctgtttgaat tgtccggtat cccttttgta
     4501 ggctgcgata ttcaaagctc agcaatttgt atggacaaat cgttgacata catcgttgcg
     4561 aaaaatgctg ggatagctac tcccgccttt tgggttatta ataaagatga taggccggtg
     4621 gcagctacgt ttacctatcc tgtttttgtt aagccggcgc gttcaggctc atccttcggt
     4681 gtgaaaaaag tcaatagcgc ggacgaattg gactacgcaa ttgaatcggc aagacaatat
     4741 gacagcaaaa tcttaattga gcaggctgtt tcgggctgtg aggtcggttg tgcggtattg
     4801 ggaaacagtg ccgcgttagt tgttggcgag gtggaccaaa tcaggctgca gtacggaatc
     4861 tttcgtattc atcaggaagt cgagccggaa aaaggctctg aaaacgcagt tataaccgtt
     4921 cccgcagacc tttcagcaga ggagcgagga cggatacagg aaacggcaaa aaaaatatat
     4981 aaagcgctcg gctgtagagg tctagcccgt gtggatatgt ttttacaaga taacggccgc
     5041 attgtactga acgaagtcaa tactctgccc ggtttcacgt catacagtcg ttatccccgt
     5101 atgatggccg ctgcaggtat tgcacttccc gaactgattg accgcttgat cgtattagcg
     5161 ttaaaggggt gataagcatg gaaataggat ttactttttt agatgaaata gtacacggtg
     5221 ttcgttggga cgctaaatat gccacttggg ataatttcac cggaaaaccg gttgacggtt
     5281 atgaagtaaa tcgcattgta gggacatacg agttggctga atcgcttttg aaggcaaaag
     5341 aactggctgc tacccaaggg tacggattgc ttctatggga cggttaccgt cctaagcgtg
     5401 ctgtaaactg ttttatgcaa tgggctgcac agccggaaaa taacctgaca aaggaaagtt
     5461 attatcccaa tattgaccga actgagatga tttcaaaagg atacgtggct tcaaaatcaa
     5521 gccatagccg cggcagtgcc attgatctta cgctttatcg attagacacg ggtgagcttg
     5581 taccaatggg gagccgattt gattttatgg atgaacgctc tcatcatgcg gcaaatggaa
     5641 tatcatgcaa tgaagcgcaa aatcgcagac gtttgcgctc catcatggaa aacagtgggt
     5701 ttgaagcata tagcctcgaa tggtggcact atgtattaag agacgaacca taccccaata
     5761 gctattttga tttccccgtt aaataaactt ttaaccgttg cacggacaaa ctatataagc
     5821 taactctttc ggcaggaaac ccgacgtatg taactggttc ttagggaatt tatatatagt
     5881 agatagtatt gaagatgtaa ggca
//
LOCUS       DACDXO010000100         5879 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E23605_2017
            SAMN12639989-rid9881463.denovo.105, whole genome shotgun sequence.
ACCESSION   DACDXO010000100 DACDXO010000000
VERSION     DACDXO010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639989
            Sequence Read Archive: SRR10023389
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5879)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5879)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741914.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:42:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,972
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 33 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 17 of 73
            Pseudo Genes (multiple problems)  :: 10 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5879
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639989-rid9881463.denovo.105"
                     /isolate="E23605_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(44..3193)
                     /locus_tag="IWM35_002575"
     CDS             complement(44..3193)
                     /locus_tag="IWM35_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HAP9920394.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWSNLRQ
                     KIISNNQAELNDSPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIERENPQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIIQIELK
                     QVTAKDGFYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNKKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKTLMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGVSSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVVDECHRALSAENMEEIKKMFPKSTWFGFTGTP
                     IFPENRKQAKGQLARTTHDQYGEVLHTYTIKNALEDGSVLGFQVEHENTVEPTSLENK
                     IYRKLKEVETYAEYSPEQINRMIDQMESVKKESYLDPTVFEAEEHIQKVIHKMFRPDN
                     AYTKFDFRNGRPTKSAILTTSSIDMAKKYYRAIKEMTKESDWLIKEFPNQPIREGRTM
                     EDPDFPRIAITYSLEENSRDSSAQQEEMQKIIEEYNQYYDTAWSLADIERYNGDINNR
                     LARKRAEFKQFGKQIDLVIVVDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTY
                     PEKTKGLIVTFRKPATMEKNVEDATKLYSEAKEESGLIYPSYQESKKRFKQAYGKLKE
                     VSSMESIDEHTPLEIRIEYVKAFQELNRAYESLVTYDDYNDDMVQSSTLNNQIQLLEE
                     QIGVYETVKGSLMEEEPEKEGEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSD
                     AREEIEKALAKLNKTEGVKEVYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDNLIHA
                     FSNEWFVSEKELHASNLQYMPGEDPIPNMKAIINSKDYEGYKAKHPEAKPFKYPQEMK
                     RAWRKMLDDELIPLENELR"
     gene            complement(3207..4424)
                     /locus_tag="IWM35_002576"
     CDS             complement(3207..4424)
                     /locus_tag="IWM35_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HAP9920395.1"
                     /translation="MSNDTRPEIRFPGFTEDWEERKLGDMMDVTSVKRIHQSDWTNSG
                     IRFLRARDIVSAAKNEEPSDYLYISEEKYNEYSKNSGKVSQGDLLVTGVGSIGVPLLI
                     TDDNPIYFKDGNIIWFKNEHKIDGNFFYYSFINNKIQKYIRDVAGIGTVGTYTIDSGK
                     KTPISLPTYDEQIKIGSFFKQLDHTIMLHQHELDLLKETKKGFLQKMFPKNGAKVPEV
                     RFPGFTGDWEERKLGEVVVFLNGRAFKQQELLDSGKYRVLRVGNFNTNDRWYYSNLEL
                     EEDKYANKGDLLYLWATSFGPEMWNEERVIYHYHIWKLKFLSKELDKQYLYTWLLTDK
                     EKIKESTNGTTMVHVTKGGIEQRTFQFPPDINEQQKIGAFFKQLDDTIALHQRKLDLL
                     KETKKGFLQKMFV"
     gene            complement(4414..>5879)
                     /locus_tag="IWM35_002577"
     CDS             complement(4414..>5879)
                     /locus_tag="IWM35_002577"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HAP9920396.1"
                     /translation="QVVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDIL
                     GYSISPEYLFNVLADQAKQATFQLNDLNKAFVQLASTYNQFNGLFDDVDLQSKKLGTD
                     EQQRNVTITEVIKKLNDVEVLGHDGDVIGDAYEFLISQFASEAGKKAGEFYTPHMVSD
                     MMAQIVTLDQKERRFFSVFDPTMGSGSLMLNVRNYLTHPDNVKYHGQELNTTTYNLAK
                     MNLILHGVDAEEMNLRNGDTLNKDWPTDEPYTFDAVVMNPPYSANWSADTTFLDDSRF
                     NRYGKLAPKSKADFAFLLHGFYHLKETGTMAIVLPHGVLFRGAAEGVIRQKLLEDGSI
                     YAVIGMPANLFFGTSIPTTVIVLKKNRQTRDVLFIDASREFVKGKNQNKLSEENIQKI
                     LETYAERKDVEKYAHLATFDEIKENDYNLNIPRYVDTFEEEEPIDMVHVGNDIKKIRQ
                     EQQVLEKELLEAISSLQTTPENEAWLQGALEVFKHEQ"
BASE COUNT         1591 a         1252 c          987 g         2049 t
ORIGIN      
        1 tactactatc tttgaatcgt acaattttct taactcttct cgtttacctc aattcatttt
       61 caagtggaat caactcatca tctaacattt tacgccatgc tcgcttcatt tcttgtgggt
      121 atttaaatgg tttcgcttct ggatgtttgg ctttatatcc ttcgtaatcc ttactgttga
      181 taattgcttt catattagga attggatctt cacctggcat atattgcaga ttagaagcat
      241 gtaattcttt ctcagaaaca aaccactcat tggaaaaggc atgaatcaaa ttatcacttg
      301 cttgtgtaaa atagcgacgt ttaatgacaa agatatcttc attactatct aaagtcccca
      361 catcaaatgc atttaaaatt tgtcggtaaa cttctttgac tccttctgtt ttattcaatt
      421 tagccaaagc tttttcaatt tcttctcgtg catctgaact attcgcagca taagttccta
      481 gtaattgatc aatataagta gaatcaatat catacaactt agtggtattt tcactataaa
      541 attcgatcgt tgaaaaatct tctccctctt tttctggttc ttcctccatc aatgaaccct
      601 tcacggtctc gtacacgcca atttgttctt ccaaaagttg aatttgattg ttcaaagttg
      661 aagattgcac catgtcatca ttgtaatcat cataagtcac taacgattca tacgcacgat
      721 tcaattcttg aaatgctttg acgtactcga tacggatttc aagtggcgta tgttcatcaa
      781 tggattccat ggatgaaacc tcttttaact taccatatgc ttgcttgaat ctttttttgg
      841 attcttggta acttggataa atcaaacccg attcttcttt agcttctgaa tacagttttg
      901 tcgcatcctc gacattcttc tccattgtcg ctggctttcg gaaagtaaca attagtcctt
      961 ttgttttttc aggatacgta cggttcgtac gtgaaaaggc ttggatcaat cctgcatact
     1021 ctagattacg atccacaaat agggtttgaa tcgtaggggc gtcaaaacct gtcaatagac
     1081 gatccactac gattactaaa tcgatttgtt tcccaaattg tttgaattcc gctctttttc
     1141 gtgccaaacg attattgatg tctccgttat aacgctcaat gtctgctagt gaccaggcag
     1201 tatcataata ctgattatat tcctcaataa ttttttgcat ttcttcttgt tgggcacttg
     1261 aatctcggct attttcttct aatgaatagg taatagcgat tcgtgggaaa tcaggatcct
     1321 ccatcgttcg tccttcacgg ataggctgat tgggaaactc ttttattaac caatcagatt
     1381 ctttcgtcat ctctttgatg gcccgataat actttttggc catatcaatg gaactcgtgg
     1441 ttaagatcgc agacttcgtt ggacggccat ttcgaaaatc aaattttgta taggcattgt
     1501 ctggacggaa cattttatga atgacttttt ggatatgttc ctctgcttca aaaacagtag
     1561 ggtccaaata actttctttt ttgacagact ccatttgatc gatcatacga ttgatctgtt
     1621 ccggtgaata ctctgcatat gtttctactt cttttaattt ccgatagatt ttattctcta
     1681 atgaagtcgg ctccacggtg ttttcatgct ctacttgaaa tcctaaaacc gagccatctt
     1741 ctaatgcatt cttgatggta taggtgtgca agacctctcc atactgatca tgggtcgtac
     1801 gtgccaattg ccctttggct tgtttccgat tctcagggaa aatcggtgta cctgtaaagc
     1861 caaaccaagt ggatttcgga aacatttttt tgatttcttc catattttca gcacttaacg
     1921 ctcgatgaca ctcatcaact acaaacacga tatgttgccc cattaatttt tggaagcgat
     1981 tggttccttt tttctcttcc tgtttctttg catatttcaa cgccgcatct agtttttgac
     2041 gggttgtaat gatcacaaca ttcgcattgg catctgccaa cagagtttca cttaactctt
     2101 tggcacttcc tgttcccacg atcaagctat tcgctttggc attaccagaa gaaaccccag
     2161 tattaaactc cgaagcaaat ttcgtaaact ctgtggtggt ctgattatct aaatctttgc
     2221 ggtccactaa catgatcgtt cgatcgatcc ccgattttcg tgctaaaagc ttagtagata
     2281 caaaactagt caacgtcttt cctgaaccag tcgcatgcca cacataccct gattgatgtt
     2341 tatttgcggc aataaacaag gcttgaatcg catgcacttg ataaggatgt aacaccatga
     2401 gggttttatt gtcttgatct tcactgacaa tcgtataatc cgcaatcaag cgatgggcat
     2461 ctgggatatt tagcacttgt ttgcaaaact catataagtt ttctaccttt ttattatctt
     2521 tcgttcgcca actaaataaa aacttaggat gcaaatcctt cggtaaggca tttgcaaaat
     2581 accgagtcgt ttgttcattg gaaaccacaa acaactgtaa ggtcgaaaag atattgttcc
     2641 gaaacatgcc ttcttcagca tattttttga tttgattaaa cgcctgataa aaaccatctt
     2701 ttgcagttac ttgttttagc tcgatttgga taatcggtaa gccattgatc aataaggtga
     2761 cgtcaaaacg tcgatcccgc gcttctatgt tacttccacg ttttgcaatt tgatggacca
     2821 cttcataagt agagattcct ccaccaatgt cttgattgga ataaaggata agtgagacag
     2881 aacccaattg cggattttcc cgttcaatcg tgatccgagc catcccattt tcacctttta
     2941 accatttcgc agcatcaaat ggggttttcg tccgtagcaa taattcggtt ttgatcgttt
     3001 caaactcttt atcggtcaaa ggagaatcat tcagttctgc ttgattattt gagatgattt
     3061 tttgacgtaa attgctccaa agatcttctt ctgattttag atccggacga tacgtccact
     3121 gattatgtcc ttccccgagg acacgaatca actgttcttc gacttccgct tcctcacggt
     3181 gactaatttt cattcttctt ccctccctaa acaaacatct tttgcaagaa gccttttttc
     3241 gtttccttca gcaaatctaa cttacgttga tgaagagcga tcgtatcatc tagctgcttg
     3301 aagaaggcac caattttttg ttgttcattt atatccggtg gaaactgaaa tgtcctttgt
     3361 tctataccac ctttagtcac atgtaccatt gtcgttccgt tagttgattc tttaattttc
     3421 tctttatcag tcaatagcca agtatataga tactgtttgt ccaattcctt agataaaaat
     3481 ttaagtttcc aaatatgata atggtagatt acacgttctt cattccacat ttcaggaccg
     3541 aagcttgttg cccataaata taagagatct cctttattag catacttgtc ttcctctaat
     3601 tctaagtttg aataatacca tctatcatta gtattaaaat tacctactct taaaacccta
     3661 tattttccag aatcaagtaa ttcttgttgt ttaaaagctc taccattcaa aaaaactacc
     3721 acttccccca acttccgctc ttcccaatct cccgtaaatc ctggaaaacg aacttctggc
     3781 acttttgctc cgtttttagg gaacattttt tgtaagaatc cttttttcgt ttccttcagc
     3841 aaatctaatt cgtgctggtg aagcatgatg gtgtgatcaa gttgtttgaa aaatgaaccg
     3901 atcttgatct gttcgtcata agttggaagt gagataggtg tctttttacc actatcaatt
     3961 gtataggttc cgacggttcc aatgcctgca acgtcacgga tatacttctg aattttgtta
     4021 tttatgaacg agtagtaaaa gaagtttccg tcaattttat gctcattctt gaaccaaatg
     4081 atgtttccat ccttaaagta aattgggtta tcatcagtga ttaataaggg gacacctatt
     4141 gatccgacac cagttacaag caagtctcct tgagaaactt tacctgaatt tttcgaatat
     4201 tcattatatt tttcttctga aatgtaaaga taatcggatg gttcctcatt ttttgcagct
     4261 gagacaatat cacgagcacg taaaaatcta atacctgagt tagtccaatc tgattgatga
     4321 attcgcttca cagaagtaac atccatcata tctcctaact tacgctcttc ccaatcttcc
     4381 gtaaaaccag gaaaccggat ttctggtcgt gtatcattgc tcatgtttga acacctccag
     4441 cgcaccttgt aaccacgctt cattttcagg tgttgtttgc agggaagaga tagcttctaa
     4501 aagttctttt tctagcactt gctgttcttg tcgtatcttc ttgatgtcat ttccgacatg
     4561 aaccatatca attggttctt cttcttcgaa agtatcgaca tagcgtggga tatttaagtt
     4621 atagtcattt tctttgattt catcaaaggt tgccaaatga gcgtatttct cgacatcttt
     4681 tctttcagcg taggtttcga ggattttttg aatgttttct tccgataatt tattctgatt
     4741 tttcccttta acaaattcac gactagcatc aatgaataat acatctcgag tttgacgatt
     4801 cttcttcaat acaatcaccg tcgttggaat cgatgtacca aagaacaagt tcgcaggcat
     4861 accgatcaca gcataaatac tgccgtcttc taataatttt tgacgaatga ccccttctgc
     4921 agcaccacgg aataaaaccc cgtgtggcaa gacgatagcc atagtccctg tttccttcaa
     4981 atgatagaaa ccatgaagaa gaaacgcaaa atctgctttg gattttggcg ctaatttccc
     5041 gtagcgatta aaccgagaat catctaagaa ggtcgtatct gctgaccaat tagccgaata
     5101 aggtggattc atcactactg catcaaatgt gtaaggttca tctgttggcc agtccttatt
     5161 caacgtatcc ccattgcgga gattcatttc ttcggcatct accccatgta aaatcaagtt
     5221 catttttgct agattgtatg tcgttgtatt tagttcttgc ccatgatatt taacgttatc
     5281 tggatgagtt aagtagttac gtacatttaa catcaaggaa cctgaaccca tggttgggtc
     5341 aaagacacta aagaatcgtc gttctttttg atccaacgtc acgatttgtg ccatcatatc
     5401 agaaaccata tgaggcgtgt aaaattctcc ggctttcttc cctgcttctg aagcaaattg
     5461 actgatcaga aattcatacg catccccgat cacatcccca tcgtgaccta aaacttccac
     5521 gtcattcaat ttcttgataa cttcggtaat cgtcacattt cgttgttgct catctgttcc
     5581 tagttttttt gattggagat ccacatcatc aaataaccca ttaaattgat tgtaggtaga
     5641 tgccaattgc acaaaggctt tgtttagatc gttcaattgg aacgttgctt gttttgcttg
     5701 atccgctaag acgttgaaaa gatactctgg tgagatcgaa taccccaaaa tatcaacgat
     5761 tgtggcaatc aggtcttctt ttgattcttc atctgaaagt aattcacgat acaacattgt
     5821 ttgtttgctt actgtgtcat attcctctaa ggattcatct gctaaaagaa ccacttgtt
//
LOCUS       DACDXO010000101         5809 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E23605_2017
            SAMN12639989-rid9881463.denovo.106, whole genome shotgun sequence.
ACCESSION   DACDXO010000101 DACDXO010000000
VERSION     DACDXO010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639989
            Sequence Read Archive: SRR10023389
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741914.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:42:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,972
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 33 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 17 of 73
            Pseudo Genes (multiple problems)  :: 10 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5809
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639989-rid9881463.denovo.106"
                     /isolate="E23605_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            450..1604
                     /locus_tag="IWM35_002578"
     CDS             450..1604
                     /locus_tag="IWM35_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C47"
                     /protein_id="HAP9920397.1"
                     /translation="MIILKPKNKIYLGLLFSLMIGIFSIGITNTYASENSNFNINSQP
                     IEEQAINFAKTDFEHSITSILLMENNTETDINYTLGSPFKMLDGENTANSSIFFPVFS
                     DADNTIKYVYTVKKNTNGEYSGSISQFLAKELQNLVDSNITDTTLFFQDNSIYYSDNG
                     QIKLLWKSPLSTEKNGVSSFTEKNDLISVNIKNKIKNFSKKQNFPAVKRVKSVSSGDN
                     FIMINWRIAETQTSFPWCAAYVEAAILCNKTDVRATSARAIMHYTYPNLNNSQIESKS
                     LNQDQIIKYANYCGVYPKKVARILNLSEVQTQLRKGNAIYMGTAGYGNYSNSRHALAL
                     FGWVNQGGYQTYYVWNPWYNYPTVISANAGPVTIPVPGGGFTWDNTITNW"
     gene            1598..2020
                     /locus_tag="IWM35_002579"
     CDS             1598..2020
                     /locus_tag="IWM35_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9920398.1"
                     /translation="MVNKPIVLVMGLVLVLFLTSCSKKQEIQKTELIPFLGTWQTKDH
                     SERLVITNLNTSKNIEQYTILVKENNHKIQIIDKNLNINEQVFIYKATNNLLTLRSNK
                     KQLDYTFSLDSPKKLVFILTTNDGTEGTAKPIEFFKAD"
     gene            3143..4075
                     /locus_tag="IWM35_002580"
     CDS             3143..4075
                     /locus_tag="IWM35_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAP9920399.1"
                     /translation="MANEIVKYKPELNTIPLRKFTPIEMNLFFSIVSRMRDKGNQKIR
                     FYFDQLKDLSAYKQTAGEVFIKDLTRTYDHLMDLRFGKKSNGGLTIERFTMFTDFEIH
                     GEADNPYVDIKVHEKALPLLNNLESWVRFSLTEFRDLNSSYAKTMFRLLKQYRTQGWA
                     EFSKEDFLELLDIPKSYRQTNINQFVLKPIKEELTPLFKGLTIRKKYGKGRGKPVIGY
                     RFTWKAEINHADDFSKGKQEDLRTKLFNIEHNGELTQEEKWRAKDRILDLPLGTHEAN
                     FKKQQQTEKEEVEKQTLSNELKQDLLGNLQNLFD"
     gene            4092..4607
                     /locus_tag="IWM35_002581"
     CDS             4092..4607
                     /locus_tag="IWM35_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HAP9920400.1"
                     /translation="MADKTIKDLAEELGVSKTAINKKVSDENRKLWFAKIGNKFVINE
                     IGQKAIKSMFFTENENSNQQPVGEKSKTENHKNANQVSELELFFFLEKQLNQKDIQLQ
                     EKDEQLKQMQKLLDQQQQLTLQANQQIKKLHEQLQLTYAEESSEKEPLSVTEETQKGQ
                     NKEKKWWQFWQ"
     gene            4903..5184
                     /locus_tag="IWM35_002582"
     CDS             4903..5184
                     /locus_tag="IWM35_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9920401.1"
                     /translation="MDILVRNINPKIITRVDKRCQELTTKTGEKWSRNKYLKVLIESD
                     FDRQLMNYKKEQFDLLLEKFIDIQTKNTETLNDYVHTNNQLIETLIGGI"
     gene            5186..5602
                     /locus_tag="IWM35_002583"
     CDS             5186..5602
                     /locus_tag="IWM35_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9920402.1"
                     /translation="MRKTSVQKHVTLYPSHIDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     EKQLNPSQRNQPKNRQKETSQTFTSKNFDPTNFLKR"
BASE COUNT         2228 a          961 c          930 g         1690 t
ORIGIN      
        1 aaaaaaaaag actgctcgaa agagcagtct caagcaaaaa ataaacgaac aaacgcaatt
       61 actcacgaac acaaacaaat acaataaaat acctaaataa tgaaaataag tgataaaaaa
      121 caagggtatt tatagtttaa caagcgctca taaaaataac aagtctttaa aaaatgacag
      181 ataaaaatgc acaaagaatc ggacacagat aaccgcgaaa aatcaagctt tagaaagttg
      241 tttttagcta gaccaatgaa aaatcacaaa gtccgcccct gtatccgttg gtagaggggc
      301 tacttggttt agtatacatt atgttaacta gctcgttaaa taacgattat gttcccaaaa
      361 caatatttta tgaactataa caaaaatttg ttatcgcata ttgtatattt taattatagg
      421 tgtatatcac ttaattttaa cacaaaggcg tgattatttt gaaacccaaa aataaaattt
      481 atctaggtct cctcttttcc ttgatgattg gaatttttag tataggaatt acaaatacct
      541 atgcttcaga aaattctaat ttcaatatca actcccaacc aattgaagaa caagcaatta
      601 attttgcgaa aactgatttt gaacactcca taacttcaat tcttttaatg gaaaataaca
      661 ctgaaactga tattaactat actcttggtt cgccatttaa aatgctcgat ggagaaaata
      721 cggcaaatag ttctattttc tttccagtat tttcagacgc tgataatacg attaaatatg
      781 tttataccgt caaaaaaaat acaaatggag aatattcagg aagtatttca caatttttag
      841 caaaagaact tcaaaatcta gtagatagca atataacaga tacaacctta ttttttcaag
      901 ataatagcat ttattattcc gataatggcc aaattaaact actttggaaa tcacctttaa
      961 gtacagaaaa aaatggtgtc tcttccttta cagaaaaaaa tgatcttata tccgtaaata
     1021 taaaaaataa aattaaaaat ttctctaaaa aacaaaattt tcctgcggtt aaacgtgtaa
     1081 aaagtgtctc ctccggagat aactttatta tgattaattg gcgtatagca gaaacccaaa
     1141 caagttttcc gtggtgcgct gcttacgtag aagctgctat cttatgtaat aaaacagatg
     1201 tacgcgctac ttcagctaga gccattatgc attataccta tcctaattta aacaacagtc
     1261 aaattgaatc aaaaagtctt aatcaagatc aaataataaa atatgcaaat tactgtggtg
     1321 tttatcctaa aaaagtagca cgaatactaa acctttcaga ggtccaaaca caactaagaa
     1381 aaggaaatgc catttatatg ggaactgctg gttatggaaa ttatagtaac agccgtcatg
     1441 cacttgcatt atttgggtgg gtcaatcaag gtggttatca aacttactat gtgtggaatc
     1501 cttggtataa ctacccaact gtaataagtg caaatgcagg gccagttaca attcctgtac
     1561 ctggaggtgg ttttacttgg gacaatacca ttacaaattg gtaaataaac ccattgtttt
     1621 agttatgggt ctagtgttag ttcttttctt aactagttgt tcaaaaaaac aagaaataca
     1681 aaagactgag ctgattcctt ttctaggaac ttggcaaact aaagatcact cagaacgtct
     1741 agtaatcact aatttaaata cttcaaaaaa tatagaacaa tataccatct tagtaaaaga
     1801 gaacaatcat aaaattcaaa tcattgataa aaatttaaat ataaatgaac aagttttcat
     1861 ttacaaagca acaaataatt tactaacact gcgctctaat aaaaaacaat tagattacac
     1921 ttttagtttg gattctccaa aaaagctcgt atttattttg acaacgaatg atgggacaga
     1981 agggacagct aaaccaattg aattttttaa agctgattga tgaatagttg tttgatatta
     2041 caaaacaata atttcaccga aggtgaaaca agcgaaactt tacaaaaagt ggctagcatt
     2101 tgctagcctt ttttgtattg cggagcgtga gttttgaggg ggattcaagg gggagtttct
     2161 cccctttgac aagcggttat ttgcaccaaa tacgaaggta tttggctagc aaatatagag
     2221 cttgccaaac cctttagtgt gttttgtaga aagggacctt atagtcactt tcacattaca
     2281 aacgatacgt cctatggacg tatcaaaagg gtgggactta tgagcaaatt aaagcaaaat
     2341 ttggctagca atccttctaa aaaatacacc tattgatcag agtcaaaata aatcagtttt
     2401 cgagtgagcg aatccgaatt tgcaaatctg aagctttgca aatgagtgtg ccggcttccg
     2461 taaaagtcta ggtaacatta gtcgatatat acataaggct ctaatttgcg ttttaaggcg
     2521 ttttaaacgt tctggtatgt aatttatacc aaagcgactt taaaacgcct taaaacgaat
     2581 tttataggtc ttgtaggacg tttcagggtt gttagggctt gccctgaccg cctgtaaggc
     2641 gctttaaaaa caagataagg gattttatct agacttgtct taaaacgccg tagatgagcg
     2701 ctcagagctt cttaaaggac tccttacgaa ctgtttttaa gcaaaggagc ctttaggcga
     2761 ctgcgcagtt caatgtgaga gcgtagcgat cacaccttta aagcgaccgt agggagcaga
     2821 ttcccagttt aggaaatttc gcgaacctgt ttggagaaaa aaagtaggaa atttttaggc
     2881 gggaaatttt gcccccctct tttataatct cttttataac ctctttttaa acctctttac
     2941 cgtcggctgt atccttactc tcccaaggcc tgtagctttt ttagggcatg aaattatctg
     3001 tttataggca tgaaattatc tgtttatagg catgaaatta tctgtttata ggcatgaaat
     3061 tatctgtttt aggtattgtt ttaagacata atatacctta aaattatgtc aataaaatta
     3121 gttagactgg agtatcaaat aaatggcaaa tgaaatcgta aaatataaac cagaattaaa
     3181 taccattcct ttaaggaaat ttactccaat agaaatgaac ttgttttttt ctattgtttc
     3241 tagaatgcgt gataaaggta atcaaaaaat acgcttttat ttcgatcagc ttaaagattt
     3301 aagtgcttat aagcaaactg ctggagaagt ctttataaaa gatttaacaa gaacatacga
     3361 tcatttaatg gatttacgtt ttggaaaaaa aagcaatggt ggattaacca tagaacgatt
     3421 tacaatgttt actgattttg aaattcatgg agaagccgat aacccttatg tggatataaa
     3481 agttcacgaa aaagctttgc ctttacttaa caatttagaa agttgggtac gtttctcttt
     3541 aacagaattt cgagatttaa atagcagtta tgctaaaaca atgtttagat tactaaaaca
     3601 ataccgaaca caaggatggg ctgaattttc caaggaagat tttttagaac tattagatat
     3661 tcctaaaagc tatagacaaa caaatataaa tcagtttgta ctcaaaccca taaaagaaga
     3721 actcacacca ttatttaaag gattaacgat tcgtaaaaaa tatggtaaag gccgaggcaa
     3781 acctgttatt ggttatcgtt ttacttggaa agctgaaatt aatcatgctg acgatttttc
     3841 aaaagggaag caagaagatt tacgtacaaa attatttaat attgaacaca atggagaatt
     3901 aacgcaagaa gaaaaatggc gcgctaaaga tcgaatacta gatcttccgt taggtactca
     3961 tgaagctaat tttaaaaaac aacaacaaac tgaaaaagaa gaagtggaaa aacaaacact
     4021 ttcaaacgaa cttaaacaag atttattagg aaatttacaa aacttattcg attaaagtat
     4081 aaggagtatt tatggcagat aaaacgatca aagaccttgc tgaagaatta ggagttagta
     4141 agacagctat taacaaaaaa gtttctgacg aaaatcgaaa actttggttt gcgaaaatcg
     4201 gaaacaagtt tgtgattaac gaaatcggtc aaaaagctat aaaatcaatg ttttttaccg
     4261 aaaacgaaaa ctcaaatcaa caaccagttg gcgaaaaatc gaaaaccgaa aaccataaaa
     4321 acgccaacca agtttccgaa ttagaactct tttttttcct agaaaaacaa ttaaatcaaa
     4381 aagatataca gctacaagaa aaagacgaac aattaaaaca aatgcaaaaa ttactcgacc
     4441 agcaacaaca actcacctta caagccaatc aacaaatcaa aaaacttcac gaacaactcc
     4501 agctaaccta tgcagaagaa tcttcagaaa aagaacccct ttctgttact gaagaaactc
     4561 aaaaaggaca aaacaaggag aaaaaatggt ggcaattctg gcaataaccc aacaattttt
     4621 attcataaaa attgttgggt tactttcttt ttttgctttt acaagcatag cgacatgatc
     4681 tcacgtcgct attttttaaa gagcataagg aaacgcggta tcactttctt acgaaaggtg
     4741 ctaggtaggc tgtgcctacc acgtttctaa gaaaaaaatt tgctatcact ttttcgccat
     4801 tgtggctagc ttatgtatag tatttcggta aaaaacagct atacaaatac tatatttgta
     4861 tgatatacat aaattataca cacataagaa aggacgtttt ctatggacat tttagttcga
     4921 aatatcaatc caaaaattat tacaagagta gataaacgtt gtcaggaact tacaaccaaa
     4981 acaggagaaa aatggagtcg aaataaatat ttaaaagtgc taatcgaaag tgattttgac
     5041 cgtcaactta tgaattacaa aaaagagcaa ttcgatttat tactcgaaaa atttatcgat
     5101 attcaaacaa aaaatactga aacattgaac gactatgttc atacaaacaa ccaactcatt
     5161 gaaacactta tcggaggaat ataacatgag aaaaacttct gttcaaaaac acgttaccct
     5221 ttatcccagt cacattgacg ttttagattc catcatcgaa aaaaatccag atatcaataa
     5281 ctactcggaa gccattcgct ttttatgtat gagttatgaa cctgatacca atacaacaac
     5341 taccagtaac caagccaaac taaatgcgat gggaaaagaa atttctatca tgaccgaaat
     5401 tatcgcagaa tttgctgatt tgtatttaaa agatacaggc gttcttatcg gtacaaactc
     5461 gaaagtatat caagaagtaa aagaaaaaat tgaaaaacag ctaaatccct cccaacgaaa
     5521 tcaaccaaag aatcgtcaaa aagaaacgag tcaaacattc acttctaaaa atttcgaccc
     5581 aaccaacttt ctaaaaagat aaaaaaataa accaggaaat ttttaatttc ctggtttatt
     5641 ttctagagag cggtcttcca ataagatttg caaatcgatc ccaatttgcg taaaaaaaaa
     5701 gctgctcgta cgagcagcta aacaagaaaa agtcagcttg ttttaagggt aaaataagtg
     5761 taacaaattt tgcgaatgca acgatctttt ttattatacg caaacaatc
//
LOCUS       DACDXO010000102         5790 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E23605_2017
            SAMN12639989-rid9881463.denovo.107, whole genome shotgun sequence.
ACCESSION   DACDXO010000102 DACDXO010000000
VERSION     DACDXO010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639989
            Sequence Read Archive: SRR10023389
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741914.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:42:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,972
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 33 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 17 of 73
            Pseudo Genes (multiple problems)  :: 10 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5790
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639989-rid9881463.denovo.107"
                     /isolate="E23605_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(99..1229)
                     /locus_tag="IWM35_002584"
     CDS             complement(99..1229)
                     /locus_tag="IWM35_002584"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HAP9920403.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDKLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKG
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
     gene            complement(1877..3079)
                     /locus_tag="IWM35_002585"
     CDS             complement(1877..3079)
                     /locus_tag="IWM35_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP9920404.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
     gene            complement(3139..3342)
                     /locus_tag="IWM35_002586"
     CDS             complement(3139..3342)
                     /locus_tag="IWM35_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAP9920405.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            complement(3484..4716)
                     /locus_tag="IWM35_002587"
                     /pseudo
     CDS             complement(3484..4716)
                     /locus_tag="IWM35_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173388.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
     gene            4879..5691
                     /locus_tag="IWM35_002588"
     CDS             4879..5691
                     /locus_tag="IWM35_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9920406.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
BASE COUNT         1717 a         1189 c          921 g         1963 t
ORIGIN      
        1 aaataaataa tataaaaggt aaataaaaaa gagtggattt ctccactcca tgaaaagccg
       61 actttttggg acgctttttt ttgccaagcc tcacattttt atagccaaac ttcttttgcg
      121 acttgctgca ataattggat tttcttccat tgttcttctt ctgttaaatg gtttccttct
      181 tctgttgaag caaacccaca ttgaggactg atacttagat tttccaatgg tacaaattgt
      241 tgtgcttctt ttattcgttc ttttattttc tccgtatctt ccaaatctgg aaacttagaa
      301 gtaactaatc ctaaaacaac tttactttct ttgttactcc aatgtcttaa aggagtaaaa
      361 tctcctgctc ggtcagaatc gtattccaaa aagaaaccat cataatttgt tgaaaacaac
      421 tcttctgcaa tgggttcata tccaccggaa aacagccaag aagaagcgtg attcccgcgg
      481 cagatatgtg ttgtgatcgt caaatcttct ggtttgtctt ccagtgcgtt attgataatc
      541 tctgtacaaa tatgggccat ttctttcgga tcaattcctt tttgaagaag tgcttctctt
      601 ttagtttgat cagctagaaa cgcccaattt gtatcatcga attgcaggta acggcaacct
      661 ttttcataaa aagcatgaat cgctgctttg taagtagagg acaagtcctc ataaaaatca
      721 gatagatcag gataaatctc cttgattttt gaagaaccat cattagatag gatctcttgt
      781 cgtaaaatca tcgtgggact agggatcgtt gcttttgcaa caaaagtatc atctgtttcc
      841 actacttctt tcagaaaagc aaaatctttt aaaaatgggt gatttggatt gaaagataat
      901 ttatctacta cccgaatatt ataagctggt gctgcttttc cgtggaatgt ttgattatat
      961 ccagtttctg gaacatagcc ttcgatacca tttagttctt ctaaaaaatc aagatgccaa
     1021 aatccacgcc gaaattcacc atctgtaatt cctttgatgc cgactgcttt ttggtcttcc
     1081 actaattttt ttatttcctg gtcttcgatt gcacgaagtt cttcttttga aagtaagcct
     1141 tcttgaactt tttttcttgc ttctttgatc gattgcgggc gtaagaaact tccgacatga
     1201 tctactgtaa atggtctatt tttgctcatt tcactcactc ttttctttgt tttttgtaaa
     1261 aaaaatcctt ttactagcaa atactagtaa aaggacgatt atactcgtgg taccaccttt
     1321 tttcagaaaa aacattcttt ctctcagcct gacaataaaa tcagtggtct tggtaacgaa
     1381 agactgttcg tttttgccta cctttcgaca aaaaatcctc taaggccatt ttcgacagtc
     1441 aacttctagc tttttacacc aaccaaagcc tctcttgaac cattttctgt ctactttcct
     1501 tctcatcgga tactattaga ataaaattag aatagcatgt ttgaaagaaa agtcaatagg
     1561 gaataagaac aaacaaaaac taatttgtta ttcttagtaa tttgtttgtg gaatacaata
     1621 aagcttttct aatatgataa ttgttcagtc gtcctttcat cttagatcac ttccttaaaa
     1681 tgatcatacc cgtttcaacc acataaaaaa ggacttaatt ttgaaaaatt tcccatcaaa
     1741 actaagtcct ttttattata gttatgcaaa tttgcttatt ttctcgctat ttccgataca
     1801 tcttgaacag gagcaattcg actgaaaaca ctggtgcaaa agcatttatt tgtcttatgg
     1861 tagaaccgtg gaaaactcag actggtaata aattgtcatt ttttgaaaga atagcgccct
     1921 cttgaacatt atcaatcgca cttaccactt gttgatctga ctttgcatat aagtgaccgt
     1981 aagttcctaa agtgatttga atattcgcat ggcccaatcg ttcttgaata gatagcggtt
     2041 gaatattctg ctcaattaga aatgcaacat gagaatgtcg tagcgcgtga agcttaatcg
     2101 gtttgacacc cgctttttta gcagcttctt ttaaccagtt cgcaaaagaa taaggtggcg
     2161 taaatgttcc gtccaactga gcaacatatt caaaatttcc aatacgtttt tgcaagtcat
     2221 accactgtct gagttttaag gaaagcttct tccccaaccc aatcgttcga taagcacatt
     2281 tagttttggg agtcgaaaag taccaatcat cttttgtatt ataaatcaat gtcttctcaa
     2341 tcgttatcgt gtttttctca aaatctacat tcgaccaaga caaagcttca agttctccaa
     2401 tccgaatgcc agtataaaag agcatttcat acacaatctt ccgatggtgg gtttgaatct
     2461 tatcatcctt caattcattc atcacttttt gaaactcact gtgttcccaa aactcaactt
     2521 tcttattctc agaaggaagc gaagaaacat cttcaacggg atttttagca atcaccccct
     2581 ctttaacagc ccgatccaat acaattttta ggtgccctcg aatccgattg atataagatt
     2641 ttgataacgt ttcgccatta acttttttcc gtgaattacg tgactctgtc tggcttagtt
     2701 caaataacca atcctcaata tcgtgacttg taatatctcg aacacgaaaa tgatagaaaa
     2761 actgcatacg cttttcgaaa atgaattgcg cattctcata tgtttgactt ttgacctgtc
     2821 tctcgtacca ttttacaaac ctatccttgt aaaactcttc gaaagtcatt tgattggtag
     2881 aacacagttg attaggaatt gtcttgttaa aatcatgtgt ttccatgtac ttgtcataag
     2941 caattttggc atctttttgt gaagcataac ctcgtttagt cttctgaacc tttcttccat
     3001 tttcatctat acctagatac gctcggaaga accatttttt tgtcttttta tcttggtaaa
     3061 tatttttaat ttttggcatc gttccattcc tccctgtact tgagaaaagc tactttaata
     3121 gtaccgcgct tgtactgttt attccgcaac ttgaatgtga aaaagatgtt caattactcg
     3181 ggatggcacc acaccaactt ggcgattatt ataaaaatca ataccttcaa tccttgcaag
     3241 atatgccttg ctctctttaa tcattcgtgc agcttgacta ggtttaaagc ccatctctat
     3301 caaaattgca cgatttacca tgaaatcaca ttttgtttcc attctctcac ctcctgattc
     3361 tttactcctt ttaaaataat cctctttata atctattgtt tctcattcga tggttgtgtt
     3421 atactcgtct tgtcgaggcg gagtacgttg cacaacttgt tgtgtaagca gtactttgct
     3481 tttttagtag cttgttttct catttgatat actttgtaat tttcgttcat aatactcaac
     3541 ctcctcctga taatactccg ggtctgcctt catattttgg agaagtttga tctgtcttgg
     3601 attcatttct ccttgttcaa taatcaaagg caacaaggaa gaatgaaacg tttgatcgac
     3661 ctgtgcaata aaaactaatg atggtgaaac accatgaatt aaccaactca tagttttctc
     3721 aaaactttga ggaattgtat ctaaagaaat ggtgattgcg ccataattgc taataaaggc
     3781 tgcccatttg gcatcaactt ttgcacctag atcatttgga tcacggtcat aaaaacaaat
     3841 tgctccatta agtaactcaa agaacagttt ggttaaatca tgggtttttg aaataatctt
     3901 tgccaagctt tgcgctttct cttttcgata ccgtagttca aagcgattct ttacttttgc
     3961 ctcttctaaa ggaattcctc gtcgcttccg ttgttcatgg tccttctgat aaaagcaaaa
     4021 acgacactga ctcttctttg agcctaaata caaggtctca ccatttgatt cattgtttat
     4081 gatatccgta ccgccatgag attcactcac ttgaaaagtg gtccacacat ggcctagtat
     4141 taccttatgc ttaagttctg gaatgctgag actccccaca aaatcatcta aagtaaaatc
     4201 aatccgtgta aagttcccct gataatctaa taccttacga aaaaactgtt gccaatttgt
     4261 ttttcgtgtt tttagccatc ttgctaagtg tttacagcct tgacctgaaa actccatcac
     4321 agttccttta accgcatcgt caatggaata ccgaatcgtg attacttgat tccacacgta
     4381 ttggccaata tagccctttg gcgcataatc cagttcctca aaacgttcaa tgggcatttc
     4441 aataatctct tcaattagct ttaaatgttg attcgagaaa aagtgaatgg ttagataatc
     4501 aatcaaaaca cggttaactg gaacgacctc ttcttgcaaa ctatccaaca caagattcaa
     4561 tttttcttca cttaattgtc tcgttccttt ttctactttc gataataact atctcgaaat
     4621 ccccactttt tctgctaaat atcgttgtgt ccaccccctt tcttctcgag aaactcgaat
     4681 ggcttcaccg agatcatgtt gattaacttg gttcatcgac aaacctcctt ctgctattta
     4741 cttgtcaaag aacctattga acttatttta agtacatttt tattgtacgc ctgtatttat
     4801 tttaagtcaa tatgttttga ctttttttaa gtacacacgt atactaaatt taagaactta
     4861 tcctgaggag gagttttcat ggagatagac aagcaagcag tcggcaatcg aattcggcag
     4921 attcgccaag aattaaagtt gagtatggag aaatttggca aactaattgg tgatttaccg
     4981 cgaagtacag tcaataactg ggaacgtggg atcaaccttc caaaaaccga aacccttcac
     5041 caaatcgcag agataggtca tgtcaccaat gaatatcttt tgtatggtga tcaagagaat
     5101 caatacattt tagaaatgct ccaaaaaaag gcaggtaagc ttgatcctaa gattgaaggt
     5161 ctcatagtag acgaaatcaa acacgctgac cttgttagtg aaaaagaaat ggatcgaatg
     5221 attgaatttt ttattacaaa tctcattcca ccaactgaac aagaccagtt tactttccaa
     5281 tgtatcgatg aaaaaaagca gctttatctt ggttcaacta attttggtaa ggaagcccag
     5341 ctctatttgc aacatgataa tcaaaatcat attctgcaca tgatgccctt taccttctcc
     5401 aactttagtg tggatcgcat actcgtctat ttagcaaatc aaaaatccta ttcctatttt
     5461 gggaaacatt tacccaagga actaggtgag aaagccatcc ttctatactc aattaatcaa
     5521 ttgaccagcg atgtgcgaat tgccccgttg gtctattcag aagaaaccgc ttcttatcag
     5581 tacacagagg acaatcaata tctactagaa cagcaatatc tttatctacc attcgtgatg
     5641 gaagtcgaga aagaacgcct gttaaatgca gcgtatccat catcaaaatg aggtgacaaa
     5701 attgataagc acgtgacaaa gaaaatactg ctaggacact ccccaatggg tagttgtcac
     5761 agtttcctgt tctttgttag ccccctgtta
//
LOCUS       DACDXO010000103         5614 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E23605_2017
            SAMN12639989-rid9881463.denovo.108, whole genome shotgun sequence.
ACCESSION   DACDXO010000103 DACDXO010000000
VERSION     DACDXO010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639989
            Sequence Read Archive: SRR10023389
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741914.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:42:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,972
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 33 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 17 of 73
            Pseudo Genes (multiple problems)  :: 10 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5614
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639989-rid9881463.denovo.108"
                     /isolate="E23605_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(59..2008)
                     /locus_tag="IWM35_002589"
     CDS             complement(59..2008)
                     /locus_tag="IWM35_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HAP9920407.1"
                     /translation="MENSTEFFKNIQTEFNHALTPMKPPKHKLVGRDDELHELSLILN
                     RPETPQAILLGEAGTGKTALASEWAERQRQDGKKVQVYKLSIGQLRVGGDDVLQTRLE
                     TLIPRLKEYERILKAEDKDNEFVLFIDEFHKVVSSFGSGNKIGGDSLKEPLANESLQM
                     ITATTRKEYENYIKRDDALGRRLQPLELNELKSDVVVDILRQWLNTSSVKDDPQASFR
                     LSQRIPDELLQEIVQANKLYREDLSEPAKSIAVMEDLIAKDRVDGNAYNRESLLQIFG
                     KYNIQLDSDFDMEKLQEELKSIKGQPLAVYTITMLFKRMKLGFDRFKGRKQPKATALF
                     TGSTGVGKTAMAKAVARGLYGDENKVLLVNMPDYKTKDDAMRFRRLIGTHVRHDPSAI
                     IVCDEYEKAGVETLDAFLSILDEGRISFTERGWDDREVHDTVSLSNTILFATTNAGAE
                     MFDSLNRYSSLSRVTNKENSNEYSTELKREYRKVASGIKQALIGEGLKPEVINRFGRI
                     VPFWALSEQTLLEIAYNEMLKSAELVRKHLNIDLRLPPRKERVYGQNFYADDLSMHLV
                     FDMASQEDSKSGGARNILHLVESEFEEAITEAIITYPNAKIFDVKLSEAGAFEGKGDS
                     NAEIIAKPSVAESKENKIVFSIGGA"
     gene            complement(2050..3108)
                     /locus_tag="IWM35_002590"
     CDS             complement(2050..3108)
                     /locus_tag="IWM35_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliS"
                     /protein_id="HAP9920408.1"
                     /translation="MEKNVNSTSTKDQYSIIGGLLGDKDKRTRHGLRIMLATFLGVLF
                     LLTGLGSYIYQLGNYNYYLVNNTTPIGSSLEFARSGAEITISDVWTDQNRDVTVVKLG
                     YSQNARKLLSTQGKNYNLYMATKEKDRPKKLEMSYGMLSTDGDAFLFLKGKLEERAYQ
                     VFIANQVSLISGDDTADDDTKVNVNEADSVTKALSQYSMNDVNNEGVISFGKKAKPVN
                     TPDNINFRINPYSDSTNVYQGSFLTKDGDIDYSKVVAVTSKNNVVNKLEDQLNASNTN
                     IKKIEATKAEYLERLQQNPDDKDSQESIRQLDSRMETEIKAINDTQKLIDQYKNADFN
                     KDSFGDMKTTFKYLTLTD"
     gene            complement(3227..3682)
                     /locus_tag="IWM35_002591"
     CDS             complement(3227..3682)
                     /locus_tag="IWM35_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9920409.1"
                     /translation="MNAINWHEFVNYMESLLDDLKSFSIADAVKHGLPVSTTMKLRGL
                     KIPDEIIKLRTKTIIETAKVVDNMNHELKGKKTTYEKDFERFLKSVIGKYLGKKSFSE
                     LRDEEIVPTPMVSKIRKNKAEGNYYLGTIAKIIDYLENKEQKKGTKKGR"
     gene            complement(4266..5069)
                     /locus_tag="IWM35_002592"
     CDS             complement(4266..5069)
                     /locus_tag="IWM35_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAP9920410.1"
                     /translation="MSQEVTEKIIDSDVDLVSEQTNAIVRYNNGLNTTALRGFKPVEM
                     DVFWAILSKMKRQGTAEVTFDFNLFRYLVKYDRREKDSFYSVLKALSDKLGTLTYRFE
                     DSDVYEQLWLFQKFRIDKQKETVTIKANEEFEFILNSIGKNFTRFELDNFTKLRSSYT
                     KELYRHLMQYRNKEWRNGYWVVKVEDFRRALSIPSSYRMTNIDKRILEQAKEEFLAPD
                     ENGIQVFETFDYEKIYAKKGRRVDRLRFTFSEPESNLPTISMHNWLEED"
BASE COUNT         1653 a         1108 c          777 g         2076 t
ORIGIN      
        1 cttacgaccc atcgttttat tctaaggttt tgtagtaaat atttcttgaa tttcatagct
       61 acgcccctcc aatcgaaaat acaatcttgt tttctttact ttcagcaacg gaaggctttg
      121 caataatttc agcattagaa tcaccttttc cttcaaacgc tccggcttca ctcaacttaa
      181 catcgaatat ttttgcgttt ggataagtaa ttattgcttc agtaatcgct tcttcaaact
      241 cagattcaac taaatgcaag atattccttg caccccctga tttagaatcc tcttgtgaag
      301 ccatgtcaaa aactaagtgc atggagagat cgtctgcata aaaattttga ccatatactc
      361 tttctttcct aggtggaaga cgtaaatcta tatttaaatg ttttcgtact aattcagcac
      421 tcttcaacat ttcattataa gcaatttcca gaagagtttg ttcggataaa gcccaaaatg
      481 gaactattcg tccaaatcgg ttaataactt caggctttag accttcacca atcaaggctt
      541 gtttgatccc tgatgcgact tttcggtatt ctctttttag ttcagtgctg tattcattag
      601 aattttcctt gtttgtaaca cgagatagtg agctatatcg gtttaacgaa tcgaacatct
      661 ccgcacctgc atttgttgtc gcaaataaaa ttgtatttga caatgaaacg gtgtcgtgaa
      721 cttctcgatc atcccatcct ctttcagtaa aacttatcct accttcatct aaaattgata
      781 aaaaggcatc gagtgtttct acaccagctt tctcatattc atcacacaca ataatcgcag
      841 aaggatcatg ccttacatga gtacctataa gtcgtctaaa acgcattgca tcatctttgg
      901 ttttgtagtc cggcatatta actaagagaa ctttattttc atcgccataa agtcctcttg
      961 ccacggcttt agccattgca gttttaccga cccctgttga tcctgtaaac agagcagttg
     1021 cttttggttg ctttcgacct ttaaaacgat caaatccaag cttcatacgt ttaaacaaca
     1081 ttgttatagt ataaactgct aacggttgtc cttttatgga ctttaattcc tcttgaagtt
     1141 tttccatatc aaaatcagaa tctagctgaa tattatactt accaaatatt tgcaatagac
     1201 tttctcgatt ataagcattt ccgtctactc gatcttttgc aatcaaatcc tccattacag
     1261 caattgattt agccggttcg gacaaatcct cacgataaag tttgtttgct tgtacgattt
     1321 cttgaagtaa ttcatcagga atcctttgcg ataatctaaa agaagcctga ggatcatctt
     1381 taacagagga tgtatttagc cattgtctca aaatatcgac gacaacatcg ctttttagtt
     1441 cattcaattc taacggttgt agccgtctcc ctaaggcatc atctcttttg atatagtttt
     1501 catattcttt tcttgtagta gcggtaatca tttgaagtga ttcatttgct aacggttctt
     1561 ttagcgaatc acccccaatt ttattgccac tcccaaaaga gctaacaact ttatgaaact
     1621 cgtcaatgaa gaggacaaat tcattatctt tgtcctcagc ttttaaaatt cgctcatatt
     1681 ctttcaatct tggaattaat gtttctaaac gtgtttggag gacatcatct cccccaacac
     1741 gcaattgtcc gattgataat ttatatactt gtactttctt tccatcttgt cgctgtcttt
     1801 cagcccattc acttgcaagt gctgttttac ctgttccggc ttcacctaac aagatagctt
     1861 gcggtgtttc cggtctattc aagatcagtg acaactcatg gagttcgtca tcacgtccca
     1921 ctaacttatg ttttggtggt ttcattggtg tgagagcatg attaaattcg gtttgaatat
     1981 tcttaaaaaa ctcggtacta ttttccatct gtttgtgttc acttcctcta aaataagaat
     2041 aatttacttc tagtcagtca aagttaggta cttaaatgtc gtcttcatgt ctccaaaaga
     2101 atctttgtta aagtcagcat ttttgtattg atcaatcaat ttttgtgtat cattgattgc
     2161 tttaatttcg gtctccattc gactgtctag ttgacgaata ctttcttgcg aatctttatc
     2221 gtcaggattt tgttgtaaac gttccagata ctcagctttt gtggcttcaa ttttcttgat
     2281 gttagtattt gaagcattta actgatcttc tagcttattt acaacattat tcttacttgt
     2341 aactgctaca acttttgaat aatcaatatc gccatctttg gtaagaaagc ttccttgata
     2401 gacattagtt gaatctgaat atggatttat tctgaaattg atattatcag gagtattaac
     2461 aggttttgct tttttcccaa aagaaattac tccttcatta ttaacatcat tcatagaata
     2521 ttgagaaagt gcttttgtaa ctgaatctgc ttcattcaca ttgactttgg tatcatcgtc
     2581 agctgtatca tcaccagaaa ttagtgatac ttggtttgca ataaaaactt gataagctct
     2641 ttcctctaat ttacctttta ggaataagaa agcatcccca tcagtactta acatcccata
     2701 gctcatttca agcttcttag gacgatcttt ttcttttgtt gccatatata agttatagtt
     2761 ttttccttgc gtagataaca actttctagc gttttgggag taaccaagtt ttactactgt
     2821 tacatctcta ttttgatctg tccacacatc tgaaatagtg atttctgctc ctgatcgagc
     2881 aaactccaat gaagacccta taggtgttgt attgttcacg agataataat tatagtttcc
     2941 taattgataa atgtagctac ccaagcctgt taataaaaaa agcactccta gaaaagttgc
     3001 aagcataatg cgtaatccgt gacgtgttct cttatcctta tcaccaagca accctccaat
     3061 aatactgtat tgatctttgg tagatgttga attgacgttt ttttccatca gtttgtctgc
     3121 cccctttcat aaaagagcaa acaatcagac gttttttcgg taattctagg ttcgggattt
     3181 tgaatcattt cggtatattc ttccccatta atatttaaca caaaaattat cttcctttct
     3241 tcgttccttt cttttgctct ttattttcca aataatcaat gatttttgct attgtaccaa
     3301 gatagtaatt cccctcagct ttgttttttc ggattttgct aaccattgga gtaggtacaa
     3361 tctcttcatc tctcaattca ctgaaagatt ttttacctag atattttcca ataacagatt
     3421 ttaaaaaacg ttcaaaatct ttctcgtaag ttgttttctt gccttttagt tcatggttca
     3481 tattgtcaac gacttttgcg gtttcaataa ttgttttagt tctcagctta ataatttcat
     3541 ctggaatttt caatcctcgt aatttcatcg ttgtactaac tggtaaccca tgctttactg
     3601 catcagcaat actaaaactt tttagatcat ctagtaagct ctccatatag ttaacaaatt
     3661 catgccaatt aatagcattc attgtctcac cccttcattt ttttaaaatg aggttcgggc
     3721 gtaacacatt tatgttatta taatatcata ataacataaa taaatcaatc agcaaattta
     3781 gatatccctt aaagtatcag caattattca acaagatatt tattttagct atttcagtca
     3841 tcataaaaga ctaattagca ataaaataac taatattatc catttatcag acgaaaagaa
     3901 aaaacttatc taattactct atattaactt ttaacaaata gttgacttgt ccaaaaatgt
     3961 taccttcaat ctcacaaaga tattaagatg tttcctacca aaactgatat caccggactt
     4021 tttattctgt gttgtccaaa agtgttacct ttagtcgatc aacaaattaa aaaaaattgt
     4081 ttttttcttg acttgtccaa aagtgttacc catagttgtt aaaatttgta tagattagcc
     4141 attctttcga atattattta gactgaaact tgcttacaaa aaaacttctt gcatttattg
     4201 tccaaaagtg ttacccatac ttttttaaag aagcaggttg tattaaaatt acaacctgct
     4261 tcttctcaat cttcttctaa ccaattatgc attgaaatgg ttggcaagtt tgattctggc
     4321 tcactaaaag tgaatctgag ccgatcaact cttcttcctt tctttgcata gattttttca
     4381 taatcaaaag tttcaaatac ctgtattccg ttttcatcag gagctaaaaa ttcttctttc
     4441 gcctgctcta gtattctttt gtcaatattt gtcattctat aacttgaagg tatacttaat
     4501 gctcttctaa aatcttcaac tttgactacc caataaccat ttcgccactc tttatttctg
     4561 tattgcatga gatgacgata caattctttt gtgtagctac ttcttagttt tgtaaaatta
     4621 tcaagttcaa aacgagtaaa gtttttgcca attgagttta agataaattc aaattcctca
     4681 tttgccttaa ttgtaactgt ttccttctgt ttatctatcc ggaatttttg aaaaagccac
     4741 agctgttcat agacgtctga atcttcaaaa cggtaggtta aagtacctag tttgtcactc
     4801 aaagctttta acacagaata aaaactgtcc ttttctcttc tgtcgtattt gaccaaataa
     4861 cgaaagaggt tgaaatcaaa agttacttct gctgttcctt gacgtttcat ttttgataaa
     4921 atagcccaaa acacatccat ttcaacaggc ttaaatcctc gcaaagctgt tgtattcaga
     4981 ccattgttat aacgcacaat tgcatttgtt tgttctgata ctaaatctac atcactatca
     5041 ataatctttt cggtaacttc ttgagacatt gattactctc caatcttctt tttgcgctcc
     5101 tttaatcatg ttaatctggt actaattaaa ggaaacaatt ttttctgctt gttacctaaa
     5161 ttaaatcaca actaaaggta acagtcaaca aagatttgtt actaatctaa ttttctattt
     5221 tttcgccttc caaatcaaga aaaccgaagg taacgtctta tcctagtgtg atacctcaaa
     5281 tgtccacttt tgttaccttt gctgtccaaa agtgttaccc ttactgtcca cttttgttac
     5341 ctttactgtc caaaagtgtt acctttatac ccctttaata cttactctcc caagagatta
     5401 agcctcccct aacagatata ttaacagata tttaacagat atattaacag atatttaata
     5461 aaggaggatt actcctctct tatttcaaaa tcttaaaaat aaaaaatctt aaaaaaaact
     5521 aacactatct aggagtttat tttgcttgtg taactttgaa taattgaaaa aaaggaaaag
     5581 gaggaagctt cagcttcccc ttttatttat attt
//
LOCUS       DACDXO010000104         5515 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E23605_2017
            SAMN12639989-rid9881463.denovo.109, whole genome shotgun sequence.
ACCESSION   DACDXO010000104 DACDXO010000000
VERSION     DACDXO010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639989
            Sequence Read Archive: SRR10023389
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741914.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:42:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,972
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 33 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 17 of 73
            Pseudo Genes (multiple problems)  :: 10 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5515
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639989-rid9881463.denovo.109"
                     /isolate="E23605_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(54..420)
                     /gene="ssrA"
                     /locus_tag="IWM35_002593"
     tmRNA           complement(54..420)
                     /gene="ssrA"
                     /locus_tag="IWM35_002593"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(591..1364)
                     /locus_tag="IWM35_002594"
     CDS             complement(591..1364)
                     /locus_tag="IWM35_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP9920411.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMGNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            1508..1858
                     /locus_tag="IWM35_002595"
     CDS             1508..1858
                     /locus_tag="IWM35_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HAP9920412.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            1839..2555
                     /locus_tag="IWM35_002596"
     CDS             1839..2555
                     /locus_tag="IWM35_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9920413.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAAVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            2555..4003
                     /locus_tag="IWM35_002597"
     CDS             2555..4003
                     /locus_tag="IWM35_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP9920414.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(4073..4582)
                     /locus_tag="IWM35_002598"
     CDS             complement(4073..4582)
                     /locus_tag="IWM35_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9920415.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(4572..5297)
                     /locus_tag="IWM35_002599"
     CDS             complement(4572..5297)
                     /locus_tag="IWM35_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9920416.1"
                     /translation="MKIIRYATARLIYYKKQTILYCLFSSFSAFLLIACLTLYHLQSA
                     LANQVQDRLSFIGASASTELLPALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNK
                     QELINWRLTGLSKTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVYEALLQRINFLVL
                     KTTELPDIRDILTSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLI
                     LSLFPFTFFIHHLQSRRPGRNET"
BASE COUNT         1867 a          964 c         1096 g         1588 t
ORIGIN      
        1 aacgttgatt taacaacgtt tttcatacat gtttatctgt ctcaaccata catatatgga
       61 gacggcggga gtcgaacccg cgtccaaaca catcgccact taaaaatcta cgttcatagt
      121 caactcattt aaggattcac tttacagctt gccgagtgac aggcattctg tatcgctagt
      181 ctgatgttct ctttttcaac ttacagacgg aaagttgaaa cgtatcccac taaagttagg
      241 acccttactc gagcacatgg gcgatgccga gaggatctac gctaactgtt tttaggcagc
      301 taaagcgtaa gagttgtttt cgtttttagc agttatattt aactgtaacg tttttacgta
      361 gacgtaacct acgaaacgca attcaagctc gactgtgcct gtcgaatccg taacgtcccc
      421 gttagctaac aagggttacg tgtatttttc atacaaaatt agtatagcat atattgggat
      481 aaattcaata aaaaagcgaa tcatatccca ttatttatat aataactgcc cctttcttaa
      541 gaaaaaataa gcgattttct taagaaaaga ggtagcttca aaaaaagatc ttaaatcaga
      601 tcaaaatact tcaatgcgtg gacaattccg ccttctgtat ttttctttgt gataaaggta
      661 gaaagttctt ttaatccgtc ataggcgttg cccatagcga ttttgttgtc gcaagcttcc
      721 aacaaggcaa aatcattcgg accatcgcca aatccaaaag taggaacttt ttccagctga
      781 agttcttttt gcagacgttt gacacctgcc ccttttgaga ttcctttctt cactgtatcg
      841 atcgagtaag gaccattccg ataaaaagtt agctcaggaa aatgttcgtg atagtattcg
      901 tcacctgatt gagataagat cagcaacata tttactgtgt tttctttaaa gcctaaagga
      961 tcaatttccg gaacatctga atgaatgaag tcgtaagcat tgatcattgt ttggctatga
     1021 ccggtacacc agattttctg atcgttatag taagaaagtt catgaccgtt caactgtaca
     1081 tgatcataca ttttttgaca ttcttctgaa gaaaatgttt gagaatagat ttcttcgccg
     1141 tctactcgaa tgaaagaacc attcatggtg accgcagaag taatgcccgc atcttccatg
     1201 atagagtgga tttctgcttc tgtacgtcct gtagcgatga cggggagaac attgttttct
     1261 tttaatgcgt tcatagcttt tgccacttcg ggtgtgattt ttgatttcgc atctagtaat
     1321 gttccatcaa gatcaaagaa agtgattgca cgataatttg acacaaataa aactcctttt
     1381 ttattggaat aagtttatct taacaaagaa tgaacaaaag acaaaccttt cgtgaagaat
     1441 tcctgaacat attggttcag tcaacaattt agagtatact ataagaagag aatttaggag
     1501 ggaaagaatg atttttttca gtattggtat cttattctta ataattggca gtctttttcg
     1561 gattctgccc tcaaaaggaa atctgccttt ttatgggtat cattcgcctc ttgccgcaaa
     1621 aacagacgct cattggcggc tggctcaaaa gacaagcgga aactggtttt ttcttatggg
     1681 cttactgatg gcattgatcg gctattattt aaagacatct gggcatacga attattttct
     1741 gattgaaatg cttctgctag tatttccaat tatgcctatt tttattatga cagaaaagaa
     1801 attacagaaa tatgacctgg aaactggagg gaacgataat gaatatttta atgattgaag
     1861 ataatcaatc tgtttctgaa atgatgcaga tgtttttctt aaatgaaggt tgggaagcaa
     1921 ctttcaagta tgatggaaaa gaagggctcg atgccttttt agaaaaccca caacattggg
     1981 atatgattac actagacctg aatttgccca cactggacgg tatgctagta ggaagagaga
     2041 tccgcaaagt ttcaagtact gtgccgatta tcatgctgac agctagagat tctgaaagcg
     2101 atcaggtgat cggcttagag atgggggcag atgattatgt cacaaaacct ttcagtcctc
     2161 tgacgcttat tgctcgaatg aaagctttgc atcgccgtag tgagttggca gaagcaagag
     2221 aagaagcagc agtttcagat gatcattttg atatcgagac tagtcatttc aagatgaata
     2281 cgaaaacacg agaagcgtat ctggacaacc ggccaattga aggactgacg cctaaagagt
     2341 tcgatctatt gtttacttta gcaaaaaaac cacgtcaagt attctctcgg gagcagttgc
     2401 ttgaattagt ctgggattat cagtactttg gtgatgaacg gacagtggac gcccatatca
     2461 agaaattacg acaaaaaatc gaaaaggtcg gaccacaagt catccaaact gtttggggag
     2521 tcggctacaa attcgatgat tctggtgttg cctaatgcgt tatttgtatc agcaactctt
     2581 agctttttgc ggaatggtcg cgttgatctt attgatcgtt ggagtttctt ttacacaact
     2641 gacaaaacaa acgattgaag aaaacaatta ccagcaactg tttggatatg ctgaatcagt
     2701 tgaaaagaca acgcaaacct atgccgatac gctcccgcag tttagtcaag atcaggcgtt
     2761 tcaaaatgca ttgctgctga cagagcaagt actgacagaa caaaatgtga actttatatt
     2821 tatcgataaa aacgaacgtg tcatttatcc gactaataca gctttactga attttacctt
     2881 aacagctgat caatggaaaa atctgaggaa tggacgtcaa gaaaaattca catcgaataa
     2941 aaatatttta ggacaagcac aggcgacctc ttacgcactt gtgccattta atttgaatca
     3001 tgaattctat ggagcacttg tagttagtca acctgcgcga aatatcgata acagcgttcg
     3061 tccaattaca ctgaacctgt tcaaaggatt tattttttcc agtatcgttg cagttattgc
     3121 cagctatgct tttgcagcat tccaagttaa gcgtatcaat cggttaagga atgcgacgaa
     3181 agaagtgacg aatggtaatt ttgatgtaca gctccccgta catgacaaag atgaatttga
     3241 tgagttagca gatgatttca ataaaatgac gaattcattg aaagaatctc aagcagaaat
     3301 tgaagaacaa gaaaatcgtc gaagacaatt tatggcagat gcttctcacg agatgagaac
     3361 acctcttacg acaatcaacg gacttctgga agggttagaa tacaatgcaa tccctgaaaa
     3421 tcagcgagaa aacgcgatta aattgatgaa aaatgaaaca gagcgtctga tccgtttggt
     3481 gaatgaaaac ttggattatg aaaaaatccg tacgaatcaa atcagtatgg tcattaaaaa
     3541 attcaatgga acagaaacat tgaaaaatat tgttgcacag ttagaagcta aagcagaagc
     3601 agcaggagat acattgacat tgaaagccgt tgatgatatt gatgtttacg ctgattatga
     3661 ccgatttgtc caaatcatgg tcaatattat tcaaaacgcg atacagttta ctgaaaacgg
     3721 ccaaatcacg attactttag aaaaaggcta tcttgaaacg atcatcacga ttgaagatac
     3781 gggaattgga atgtctgaac aacaaatgaa aagtatctgg gatcgttatt ataaagtaga
     3841 tccttcaaga aaaaatacaa aatatggtga atcaggatta ggtttaccaa tcgtgcagca
     3901 acttgtacgc ttgcataaag gaaaattaga agtagaaagc gaactaggaa aaggaacaaa
     3961 attcgctgtt cgtttgcctg acgtagaaat caaaaaagag taaaaaacaa gcggtataaa
     4021 agatcatgaa tcgatcttct ataccgcttg tttttactat aaaatgattg atttagcccg
     4081 cgggctctaa acgatcaata taaggacttt gagcaatagt agcatcatga gtaaagagta
     4141 agataccttg attttcttca tgagctaaat aagaaagcaa gtggagtagc tcttggatct
     4201 ccaatacact aagttcatca aatgtatctt caataacaat attgtttcgc ttggaaagca
     4261 aggcatgaag taattgcagc tttacttttt caaaagacgt caactcagct gccgcacaat
     4321 taagcaagga aggagagata tcaagttttt cttgataggc caaacgatct tgtttgattt
     4381 ctttttccgg tacatccaac aggacatggt ctttaagatt cagataagga aacatcggcc
     4441 aatttttttg gatgatcgca aaattatcca gttttgcaac aagaacctct tttttcaagt
     4501 gctccaatat tttatgctgt ttttcgagag gacacaaaat aacggtcaag tgttcagcat
     4561 gaatcaaata ttcaggtttc attacgtcca ggcctcctac tttgcaaatg atggataaaa
     4621 aaagtgaaag ggaatagact aagaatgaga cagcatccac tgagcccaat aagagtttgg
     4681 gcaaatccct taagtatatc tgtgaaaaaa agatcatttt ggaaatcgat ggtaaaaaaa
     4741 gtctgttgat ccatcggaat tgtaagtccg cttaccgaag tcaagatatc acggatatct
     4801 ggcagttcag tagtctttaa gacaagaaaa ttgatccttt gaagaagcgc ttcataaact
     4861 gactggaagc ttacgatcca caataaaacc aagatacaac aaagaaagag aggaaagaac
     4921 agctgccaga aaataaaaag gaataatttg gttttagaaa gaccagtcag gcgccaattg
     4981 atcaattcct gcttgttttg cctgactgaa tagtaaaaga aaaagataaa aaggaaggca
     5041 aatagaaaaa ataaactgaa tatgcctttt atataaaatc ggctagctaa ttctaaagcc
     5101 ggaagaagct ctgtagatgc agaagcgcca ataaatgaaa gccgatcttg gacttgattg
     5161 gctaaagccg attgcaaatg gtaaagagtc aggcatgcta tcaataaaaa agcagaaaag
     5221 cttgaaaata aacaatagag tattgtctgc tttttgtaat aaattaatcg agcagtagca
     5281 tagcggatta ttttcatact tttcacctct ataaaaagta tagaaaatga atatgaataa
     5341 agtatgaaat tcctttagtg aaatcacgaa ttaaaaccag aaaatatttt gtaaaaagca
     5401 agaggttgtg aagaagctgc cctattctat taccgtagat gcaagctttt gaacactgtt
     5461 tattcgaaaa gaggagcagc gacaataatt ttgtcgctgc tccttttaaa tttat
//
LOCUS       DACDXO010000105         4788 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E23605_2017
            SAMN12639989-rid9881463.denovo.111, whole genome shotgun sequence.
ACCESSION   DACDXO010000105 DACDXO010000000
VERSION     DACDXO010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639989
            Sequence Read Archive: SRR10023389
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741914.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:42:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,972
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 33 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 17 of 73
            Pseudo Genes (multiple problems)  :: 10 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4788
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639989-rid9881463.denovo.111"
                     /isolate="E23605_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            564..989
                     /locus_tag="IWM35_002600"
     CDS             564..989
                     /locus_tag="IWM35_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9920417.1"
                     /translation="MICKNKKKIIAIIASFSLIFSTACTVLLTNNANVEAASSSYKHN
                     HAGYRCYKMSYYVSPSKCKQLARQTNKLNTVSAIANFIGLSGLTPGIISLVFGSAVSA
                     NNVFVTAARRGKGVQLNYIAHFSNTTTYNYSSNQNYVIK"
     gene            2141..3118
                     /locus_tag="IWM35_002601"
     CDS             2141..3118
                     /locus_tag="IWM35_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAP9920418.1"
                     /translation="MANEIVKYHHELNTIPLRKFTSVEMNLFFSIVSRMRDVGDKKVQ
                     FTFEQLKDLSNYKATANVRFIDDLETTYDKLMDLRFGRRSADGLQRERFVLFNQFKID
                     GKADIPFAEIQVHEKALPLLNNLEEWVRYSLQQFNELESSYSKTMFRLLKQYRTKGIA
                     YFSKEDFHELLDIPKSYRESDINKKIIKPIRQELTAIFKGLTIKKKYGKGRGKPVIGY
                     QFTFKPEIKKADDFNKHAPTKEEQNKALELVNQQIYSEKDPTSLQEKIKEQKRKLELL
                     QQLEELERKQNNLNKENKEINEQIPDNNSDIPPELKKGLLDNLQNLFKE"
     gene            3128..3625
                     /locus_tag="IWM35_002602"
     CDS             3128..3625
                     /locus_tag="IWM35_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9920419.1"
                     /translation="MTDKTIKELADILGVSKDKVKYQLRKLPSNFTYKKGNIIYLKKE
                     AVTTITKVLSGNEPGNLPSELPDSLPTDYLLKEIETKNNHIEEKDRQIEKLQLALETQ
                     QKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENKSTTLSEETESIEKQSKTEKKW
                     WQFWQ"
     gene            4001..4282
                     /locus_tag="IWM35_002603"
     CDS             4001..4282
                     /locus_tag="IWM35_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9920420.1"
                     /translation="MDILVRNINPKIITRIDKRCQELTTKTGKKWSRNQYLKILIEND
                     FDHELMNYKKDQFDLLFEKFIAIQAENTNILNDYVHTNNQLIETLIGGI"
     gene            4284..4700
                     /locus_tag="IWM35_002604"
     CDS             4284..4700
                     /locus_tag="IWM35_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9920421.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDVNNYSEAIRFLCMSY
                     EPATNTTTTSNQAKLNAMGKEISIMTEIMAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     ENQLNPSRRNQPKNRQKETNQTFTSGNFDPTNFLKR"
BASE COUNT         1757 a          816 c          786 g         1429 t
ORIGIN      
        1 aaaaaaaaaa gaaaaatccg cttctgcgaa ttcttctttt ttcattttgg ggcgctgccc
       61 taatccctaa gtcccggtta ttcaatagca tggaaactca aagaataaga cgttccctgg
      121 aataaaaaaa aggctgctcg ttcgagcagt ccatacatag gaaaatgaaa aaacgacgtc
      181 ccttactgag aaacgctatt aaacttttaa caaatcctct aaaaaataac aagtctttaa
      241 aaagtgaccg gtaaaaatgc acaaagaact aaaaaagaga aattgcatca aatcaagcat
      301 tagaagctag taactagcta gaccaattga aaatcacaaa gtgagcgcct gtatctattg
      361 ctacaggcgc tacttggcat agtgtacatt atgtcaacaa gaatttaata taacatattt
      421 ttctagtttg ttataacaat gccactctaa caaaaatgta agcattatcc ttattaaaag
      481 ctccttgaca aggactatac cttttatttt atgattttta catagaaaac gttttctcta
      541 ttttttgttt agaggtgatt tttatgattt gcaagaacaa gaaaaaaatt attgctatta
      601 ttgctagttt ctctttgatt tttagtactg catgtactgt cttattaaca aataatgcta
      661 atgttgaagc tgcaagctct tcttacaaac ataatcatgc tggttataga tgttacaaaa
      721 tgagttatta tgtaagtcct tctaaatgca aacaattggc tcgccaaact aataaactta
      781 ataccgtctc agctattgca aactttattg gactatctgg attaacaccg gggattatat
      841 cattagtttt cggatcagca gttagtgcta ataatgtatt tgttacagcc gctagaagag
      901 gaaaaggcgt tcaattaaat tatattgccc attttagtaa taccactacc tataattata
      961 gttctaacca aaattatgtt ataaaataat taaaacttta aactaggagg aatgctttat
     1021 gaaattaaca aaaattgctg taattttagc tgttattgct ggcattattt tatattttgt
     1081 attgcctgct aatcaattct tcctttactt tggaatacct gcgattttaa tagtaaatgg
     1141 cgtaagaatc tttgaacaac gaaaaaaatg tagctctacc taatcgttac aaaacaacaa
     1201 tttcaccttc ggtgaaataa gcgaaacttt acaaaaagtg gctagcaaat gctagccttt
     1261 tttgtattgt agagcgtgaa ttttgagggg gattcaaggg ggagcttgcg cccctttgac
     1321 aagcggttat ttgcaccaaa tacctccgta tttggctagc aatccttcta aaaaatacac
     1381 ctatcgatca gagtcaaaac aaatcaattt tcgagtgagc gaatccgaat ttgcaaagct
     1441 gaagctttgc aaatgagtgt gccggcttcc gtaaaaatac atatggctat aatttgcgtt
     1501 ttaaggcgtt ttaaacgttc tggtatataa ttataccaaa acaactttaa aacgccttag
     1561 atctaatttt agaggtctta tagaacattt tagggttgtt agggcttgcc ctgaccgcct
     1621 gtaaggcgct ttgagaacaa gataagggtt ttatactaag actgtcttaa aacggcttaa
     1681 acaaacgctc agaacttttc aaaggagttc tttatgaact gtttttaagc aaaggagcct
     1741 ttaggcgact gcgcagtata atgtgagagc gtagcgatca cacattttta gtgaccgtag
     1801 ggagcgagtt tcccagttta gaaaaattcg cgaacttgtt tgaggaaaaa agcaggagtt
     1861 ttttaggcgg gaaattttgc ctcctctttt ataatctctt tttaaacctc tttttaaacc
     1921 tctttaccgt cggctgtatg cttactctcc caaggtgtgc agaatctttt gggggtcttt
     1981 ttgtctgttt ttaggggtct ttttgtctgt ttttaggggt ctttttgtct gtttttgggg
     2041 gtctttttgt ctgtttttag gggtctttaa ttaggggctt gacccctatt agaaagctct
     2101 ttataatttt gttaagtagt taatcttgaa ggaggatttt atggcaaatg aaattgtgaa
     2161 ataccaccat gaattaaata cgattccttt acgcaaattt acgtcagtag aaatgaattt
     2221 attcttctct atagtatcta gaatgcgtga cgtaggcgat aaaaaagtac aatttacatt
     2281 cgaacaatta aaagatttaa gcaattataa agcaactgca aatgttcgat tcatagatga
     2341 tttagaaaca acgtatgaca aattaatgga tttacgtttt ggtagaagaa gtgctgacgg
     2401 tttgcaacgt gaacgatttg ttttatttaa tcaatttaaa atcgatggaa aagctgatat
     2461 tccttttgct gaaatacaag tccatgagaa agccttaccc ttacttaata atcttgaaga
     2521 atgggttcga tattctctcc aacaattcaa tgaattagaa agcagttatt ccaaaaccat
     2581 gtttagatta ttaaaacaat acagaaccaa aggaattgcc tatttttcaa aagaagactt
     2641 tcatgaatta cttgatatcc ctaaaagtta tagagaaagt gatataaata aaaaaattat
     2701 aaagcctatt cgccaagaac taaccgcaat cttcaaaggt ctgacaatca aaaagaaata
     2761 cggaaaagga agaggaaaac ctgtcattgg ctatcaattc acgtttaaac ctgaaataaa
     2821 aaaagcagac gactttaata aacacgcacc aacaaaagaa gaacaaaata aagctttaga
     2881 actagttaat caacaaattt actctgaaaa agaccccact tcattacaag aaaaaatcaa
     2941 agaacagaaa cgaaaattag aacttttaca acaattagaa gagctagaaa gaaaacaaaa
     3001 taatctgaat aaagaaaata aggagattaa cgaacaaata ccagataaca attccgatat
     3061 tcccccagaa ttaaaaaaag gcttactaga taatcttcaa aacttattta aagaataagg
     3121 ggtttttatg acagataaaa ctatcaaaga attagcagat attctaggtg tttcaaaaga
     3181 taaagtgaag tatcaactga ggaaattacc tagtaatttt acctacaaaa aaggaaatat
     3241 tatctacctg aaaaaagaag cagtaacaac aattacaaag gttttatcag gtaatgagcc
     3301 gggtaactta cctagtgaat tacctgattc attacctacc gattacctac tcaaggagat
     3361 tgaaactaag aataatcata ttgaagaaaa agataggcaa attgaaaaac ttcaactagc
     3421 tttagaaaca caacaaaaat tactcgacca gcaacaacaa ctcaccctgc aagccaacca
     3481 acaaatcgag aaactgcagg aacaactaca gttgacttat acggaagaat cttcggaaaa
     3541 taaatcaacg actctttctg aagaaacaga atcgatagaa aaacaatcaa agacggagaa
     3601 aaaatggtgg caattctggc aataaaacca gcaatttaaa ttaataaaaa ttgctggttt
     3661 tattcttctt tttgcttttg cgagcagagc aacatgggcc catgttgcga tattttggtg
     3721 gcgccccaat ccccgttttt tcgttgaatc ttactcggac tgcactcgtt ttacagctga
     3781 tttttctgaa ttttgaaaga gcataaggaa acgtggtatc actttcttac gaaaggtgct
     3841 agataagctg tgcctatcac gtttctcaga aaaaaatttg ctatcacttt tttgccattg
     3901 tggctaggct atgtatagtg ttttggcgaa aaatagctat acaaatacta tatttgtatg
     3961 atatacataa attatatact gatacgaaag gaaaatttcc atggacattt tagttcgaaa
     4021 tatcaatccc aaaattatta caagaataga taaacgttgt caggaactaa caaccaaaac
     4081 gggcaaaaaa tggagtcgaa atcaatacct aaaaatttta atcgaaaacg attttgacca
     4141 cgaacttatg aattacaaaa aagatcaatt cgatttatta ttcgaaaaat ttattgccat
     4201 tcaagcagaa aatacgaaca tactaaatga ctatgttcac acaaacaacc aactcattga
     4261 aacacttatc ggaggaatat aacatgagaa aaacttctgt tcaaaaacac gtaacccttt
     4321 atcccagtca cgttgacgtt ttagattcca tcatcgaaaa aaatccagac gtcaacaatt
     4381 attcagaagc cattcgcttt ttatgtatga gttatgaacc tgctaccaat acaaccacaa
     4441 ccagtaacca agcaaaactc aatgccatgg gaaaagaaat ttctatcatg acagaaatca
     4501 tggcagaatt tgctgattta tatctaaaag atactggtgt tcttatcggt acaaattcta
     4561 aagtatatca agaagtgaaa gaaaaaattg aaaatcaact caatccttcc cgacgaaatc
     4621 aacccaaaaa tcgtcaaaaa gaaaccaatc aaacctttac ttctggaaat tttgacccaa
     4681 caaactttct aaaaagataa aaaaagaaac cgaaaaataa aaaaattcct cgatttcttt
     4741 ttagggatcg gtcttccagc aagatttgca agttggtacc aacttgcg
//
LOCUS       DACDXO010000106         4744 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E23605_2017
            SAMN12639989-rid9881463.denovo.112, whole genome shotgun sequence.
ACCESSION   DACDXO010000106 DACDXO010000000
VERSION     DACDXO010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639989
            Sequence Read Archive: SRR10023389
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741914.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:42:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,972
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 33 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 17 of 73
            Pseudo Genes (multiple problems)  :: 10 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4744
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639989-rid9881463.denovo.112"
                     /isolate="E23605_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            25..696
                     /locus_tag="IWM35_002605"
     CDS             25..696
                     /locus_tag="IWM35_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP9920422.1"
                     /translation="MNFLVVLKDTLIERSTFLYVQLQENKTLLHFSPEFHLEGLTLGE
                     IYQAEGLSAVLHFFKVELELPVKDYIELSLESWDRAVVELFPEGLMIDTNDGKIHLTA
                     AELQKQMRYQIDDENSFSIFRRQQKILKSFLKVISKKRNLIKTTQLLKKYPGLLHTSI
                     QFPQLITMIHRFIRMQQLPSKKLFLPLTDTFRVVNREDKKKVIVIDFTRNRNVLHQVA
                     MEKAN"
     gene            755..1195
                     /locus_tag="IWM35_002606"
     CDS             755..1195
                     /locus_tag="IWM35_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9920423.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            1207..1875
                     /locus_tag="IWM35_002607"
     CDS             1207..1875
                     /locus_tag="IWM35_002607"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HAP9920424.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            1939..2562
                     /locus_tag="IWM35_002608"
     CDS             1939..2562
                     /locus_tag="IWM35_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HAP9920425.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            2618..3004
                     /locus_tag="IWM35_002609"
     CDS             2618..3004
                     /locus_tag="IWM35_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HAP9920426.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            3217..4608
                     /locus_tag="IWM35_002610"
     CDS             3217..4608
                     /locus_tag="IWM35_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HAP9920427.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
BASE COUNT         1580 a          782 c         1011 g         1371 t
ORIGIN      
        1 aatgatgaaa aaagggggag aaaaatgaat tttttagttg ttttaaaaga tactctgatt
       61 gagcgatcta catttctata tgttcaattg caggaaaata aaacattgct gcatttttca
      121 ccagagttcc atctagaggg gctaacatta ggagaaatct atcaagcaga agggctgtcg
      181 gctgttttgc acttttttaa ggtagaattg gaattaccag taaaagatta tatcgagctt
      241 tctcttgaat cttgggatcg agcagtggtt gaactgtttc cagaaggttt aatgatcgat
      301 acaaacgatg ggaagatcca tctgactgca gcagaactgc aaaaacaaat gcgttatcaa
      361 atagatgacg aaaattcttt ttctattttt agacgtcagc aaaagattct aaaaagcttt
      421 ttaaaagtga tcagtaaaaa acggaatctg atcaaaacga cacagctgtt gaaaaaatat
      481 ccaggtctgc ttcacacgtc tattcaattt cctcaattga ttacgatgat ccatcgtttt
      541 atacgtatgc aacagctacc atctaagaag ctgtttcttc cattgacgga cacatttcga
      601 gtagtcaaca gagaagataa aaagaaagtg attgtgattg actttaccag aaatcggaat
      661 gtccttcacc aagttgcaat ggaaaaggcc aattaaggct ttttcttttt ggattttttt
      721 gtcataataa atattagatg atggaggaga taatatgatg caaacatggt tagaaaggct
      781 aatacgattt cagcagaact atcacaaact tatgcaggga agatatgcga aatttgatca
      841 gttgaatcgc ggtctattga tcgctgcgct tgtctttagt ttgctaagtc gttggttgcc
      901 ctatagaatc ggacaattgt tgttcatcgt ctttttcgga tggatgatat ttcgtttcct
      961 atcaaaaaaa atctacccca gactaaatga gaatcaacgt tatttgaaat atcttgatag
     1021 agtcaaagaa aaatatcatc agataaaaaa caaaaagcaa gtcaaaatgg atcgaaaaag
     1081 ttatactttt tttgagtgcc cgaattgtca tcaaaagcag cgtgcaccta aaggaaaagg
     1141 acgaatacgt gtgacatgca aaacatgtgg tataaaattc gaaacgaatg tttaagggag
     1201 aaaagaatga aattagtatt attacgtcat ggcgaaagcg aagcaaattt tgaaaattac
     1261 tggacaggat ggctagatgt cgccttaaca gaaaaaggaa aagaacaagc agcagttgcc
     1321 ggtcaaaaaa tgcttgaagc cggacttcaa ttcgatgccg tttatacctc tgttttgaaa
     1381 cgtgcgattc ttaccggaca agtcgcacta gaagaaatgg gtcagttatg gcttcctata
     1441 ataaaaacat ggcgtctaaa tgaacggcat tatggggatc ttgtgggtaa aaacaaagat
     1501 aagatgaaag aaatatacgg aaaagatcaa gtaaaaacat ggcgcagagg atatacggaa
     1561 gttccgccgt taaccgcaga aaatagattt gaccgcagat atgatcactt ggatccgcat
     1621 ttgatcccat acggagaaag tctggaaatg acagtgaatc ggatcattcc cttatggcaa
     1681 gatcatctag caccaaaact aaaagatcgg caagatttac tagttgtggg tcatggtaat
     1741 agcatccgag cactgacaaa atatttagaa gatgttcctg aagatcaaat ggatacgatc
     1801 gatatcccaa atgcacaacc aatccagtat acattagcag atgatttgac aattctggac
     1861 aaaaaaatac tttaaagaac acctttatct aaaagtgaga gatagaaaaa agaagcaaag
     1921 gaatggaaca aaaggaacat gaaaaattat atatgggact ttgatggtac attgtttgat
     1981 acgtatcccg caatggtaga tggtgcatgg caagcactaa aagattttgg tatcagtatg
     2041 gacaaaaaag aaatctactt caaaatgaaa aagtattcta ctagttattt gatcaatgaa
     2101 tcaaacttga atgccagaga gtttaatgaa ttatttcacc gatatgaaaa agaaagtacg
     2161 gaagtttccc gtccatttcc agaaacgaaa caagtactgg aaatgctaaa agacaacggc
     2221 ggtcgtcatt tcatcctgac ccaccgtttg acagaatcaa catggggcct gttaaaagaa
     2281 catcgattgg cacatctgat tgaggaagta gtagggatcg atcaggactt tcctcgtaaa
     2341 cctgatccag catctttaaa ttatttgatc gatacttttc atttagaaag aacagacacg
     2401 atgatgatcg gcgatcgtcg tttagatatc gaagcaggga aaaatgcagg agtcgctact
     2461 tgtctttacg acatcgatca ttttctgggt gagatcccgg cagattatgt ggtaggaaat
     2521 ctgaatgaaa tccttacctt gcagagcatc attaaaaagt agtatcccct ttcaaaggag
     2581 atatgttata attttcataa aggagtgata aaattatatg gaaatcagaa gacaaaaaga
     2641 agttgtagaa gcatttcatt atgatatgag attgccgaac caagaagtag aaacagattt
     2701 gaaagtaggc ttctcgcctt taaaagaaac gatcgaaaac tatccgaaag aacatagcat
     2761 cgtaggagca cgcttagagt tccgtttagt gtttgataca tatgttttat ctggaagtgt
     2821 gagtcagatc aatcatatcc tcaatcggaa aattgaaggt caaaatgatg tcacacaaga
     2881 agaagtggac gagctagtag caccgttatt cgatatcgta cgccgaatgt cttatgaggt
     2941 gacagagatc gccttagatc agcctggggt aaaattgaat tttcaatcag aaaaacaaga
     3001 atagacagta aaaacatacc tgcgatagat taaagctatc acaggtatgt ttttttatta
     3061 ctaattgtaa gcaaaaagaa tgtcatttcg ataaaaatcg tctaaaatat aaaagtcact
     3121 gaaaaaaatt ttttcaggag gatggttgaa gaatcattct cgggaacctt tacttccttc
     3181 gatggacttg tcattaataa tatgaggagg agaaaaatga caacactcat cacagtttta
     3241 gtggttctag tttttgcggc attattatat gtgttctatc gtatgcaaaa gcgccataca
     3301 aaattttcaa cacgcgtctt tacagcatta ggagttggta tcgtctttgg cggtctgctt
     3361 caattgatct ttggtgtaga cgacaaagtc gttactcaat caatggaatg gatcgggatt
     3421 gtaggtcaag gatatgtatc attcttgcaa atgctagtta tgcctttagt gtttatttcg
     3481 atcattggtg cattcacaaa aatgaaagtt tctgaaaagc tagggaaaat tagtgcgact
     3541 gtattgactt ctttacttgg aacaacagct attgcagcat tgatcggaat tggttcaaca
     3601 atgctgttcg gactccaagg agctaaattc acacaaggag ctgcagaaac agcacgaatc
     3661 tctgaactgg caacacgtca ggaaacagtt gaaaacttat cgattccaca gcagatttta
     3721 tcctttatcc ctaaaaacgt ttttgctgat ttagcaggta gtcgtgcaac aagtacgatt
     3781 ggagtcgtta ttttcgcggc atttgtcgga atcgcttatc taggcgtgaa aagaaaagcg
     3841 ccaaaagaag cagcattctt ttcagattta gtcgacagtc tgtataaaat cattatgcgg
     3901 atcgtgacgc ttgttttgcg tttgacacct tatggagtat ttgctttgat gacaaacgtt
     3961 ttggcaacaa gtgattttgc agctatcgtc aatttaggta aatttgtcgt tgcttcttac
     4021 gtagcattgc ttgctgtttt gattgtccac tcactgattt tgattggtgc aaaagtcaat
     4081 ccaatcaact atttcaaaaa agcctttccg gtattgagct ttgcgtttac ttcacgttca
     4141 agtgctggag ctttgccatt gaatatcgag actcagacaa aagctttagg agtagatgat
     4201 gcaacagcta atttctcagc aagttttggt ttgtctattg gacaaaacgg ctgtgctggc
     4261 gtttatccag ctatgttggc aacgatcgtc gcaccagcag ctggagtgaa tgtctttagt
     4321 ttggaattta ttttgatgct tgtggcaatt gtaacaatca gttcttttgg tgtagctgga
     4381 gtcggtgggg gcgcaacatt tgcttcactg atcgttttag gggcaatgaa cttgccagta
     4441 gcaatcgttg ggttagttat ctctgttgaa ccattaatcg atatggcacg tacagcagta
     4501 aacgtcagcg gtagcatggt agctggtatc gtgacaagtg cacgcatcaa agatttagat
     4561 cgcgatgtca tcaatgatga ttctaaagtg atcgatgtga atgcatagtc aaaaagaggt
     4621 tgtgatagaa gtggtcagct ttaagaaata agaagaagtt tcctaaaaat agtttctcaa
     4681 atttttgtga aatttcggct tatttccgag aagctacttc tgaaacaccg tgaataagag
     4741 gttg
//
LOCUS       DACDXO010000107         4720 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E23605_2017
            SAMN12639989-rid9881463.denovo.113, whole genome shotgun sequence.
ACCESSION   DACDXO010000107 DACDXO010000000
VERSION     DACDXO010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639989
            Sequence Read Archive: SRR10023389
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741914.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:42:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,972
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 33 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 17 of 73
            Pseudo Genes (multiple problems)  :: 10 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4720
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639989-rid9881463.denovo.113"
                     /isolate="E23605_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..1097)
                     /locus_tag="IWM35_002611"
     CDS             complement(<1..1097)
                     /locus_tag="IWM35_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAP9920428.1"
                     /translation="MARKKRNPDAEKLAESILNTYQPESVEDMQDVLKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKKHDNRRNGYGNKTLKTSFGKVNIDVPRDREASFEPELIP
                     NRKRDVSDIEGKVLSMYARGMSQCDIAATVEDIYGFDISHEMISDITDAVLPELEEWQ
                     ARPLAKCYAFLFVDCMYVTLRENYEAKEYAVYTILGYDLKGNKEILGLWLNQTESKNR
                     WMQVFDELKVGGVEDVFFISMDGVSGLKEGAKAIFPSVIVQRCIVHLVRNALRYLPSK
                     DYKEVCRDMKKFYSASSLNAAHAAFDSFQNRWSHYSGAVDVCKRNFSHVEQLFDYGSA
                     IRKIMYTTNAVESIHSSFRKVTKKGVFSNENA"
     gene            complement(1227..2603)
                     /locus_tag="IWM35_002612"
     CDS             complement(1227..2603)
                     /locus_tag="IWM35_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9920429.1"
                     /translation="MIKNAFAYVTRKSLKSLIIILVILSMSTLSLISLSIKDATDRAS
                     KETFANITNSFSMEINRQVNPGTPRGGGNVKGEDIKKISQTDSIDSYVKRINSVADLV
                     DYDIIETQETLANQSPERAKNFKRTVMLTGVNDSTKETKFVSEAYKLVEGKHLENEDK
                     NKILMHKDLAEKNNLKVGDKIKIKSNLFDADNEKGADETVEVEIKGLFDGHNSGGVSA
                     AQELYENTLITDIHTAAKVYGNTEDTAVYQDATFFVKGDKNLDSVIKDLGKLDINWRE
                     YNLIKSSSNYPALQQSISGIYSISNKLFFGSLIFAGVVVSLLLFLWMNARKKEIAVLL
                     SLGISKLEIFGQFLIEMVFISIPAFVGSYFLAQYTADKLGNNILNKVTGDIAKQIAKQ
                     SASSQLGGGAEAEGFNKTLSSLDINVLPKFMIYVVIFMSLVLLVSLIISSFNILRRNP
                     KELLIDNN"
     gene            complement(2603..3259)
                     /locus_tag="IWM35_002613"
     CDS             complement(2603..3259)
                     /locus_tag="IWM35_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9920430.1"
                     /translation="MDILEIKNVAYSYANSKEKVLSGVNQKFELGKFYAIVGKSGTGK
                     STLLSLLAGLDKPQTGKILFKNEDIQNKGYSNHRKNNISLVFQNYNLIDYLSPIENIR
                     LVNKSADESILFELGLDKKQIKRNVMKLSGGQQQRVAIARALVSDAPIILADEPTGNL
                     DSVTAGEIINILKTLAKDRNKCVIVVTHSKEVADSADIILELSGKKLKKVNKMNLEVE
                     "
     gene            complement(3269..4546)
                     /locus_tag="IWM35_002614"
     CDS             complement(3269..4546)
                     /locus_tag="IWM35_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9920431.1"
                     /translation="MIKNAIAYITRKKNRTLIIFIILTIVLSCLYSCLTIMKSSNEIE
                     KALYESSNSSISITKKDGKYFNVNQFKDIEKLKEIEEKIIQYDGLAKLKDAKVVSGEQ
                     RINREDLSDEFKNVVSLEATNNTKRNILFSSGVFTIKEGKNIEENDKNSIIVHEEFAK
                     QNNLKLGDEVDLELLDIEKSGKIKSHKFKIIGIFSGKKQETYTGLSSDFSENMVFVDY
                     STSQEILNNSENNEIANKILMYSGSAESTDLALNKLKELKIDESKYFVEKDSNAFEES
                     LESVSGIKYIIKVMTYSIMLGGMVVLSLILILWLRERIYEIGIFLSIGTSKIQIIMQF
                     IFELIFISIPSIISSLFLGNVLLKVIVDGFINSEDSMISGGSLINNSSFMSNITTLGQ
                     SYLILISIIVLSVVFASSLMLIKKPKEILSKIS"
     gene            complement(4565..>4720)
                     /locus_tag="IWM35_002615"
     CDS             complement(4565..>4720)
                     /locus_tag="IWM35_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4Fe-4S)-binding protein"
                     /protein_id="HAP9920432.1"
                     /translation="DKDKCINCGKCKKVCQMNKDSVENCNHLECIRCGRCKNACPVDA
                     ISYGVRK"
BASE COUNT         1477 a          858 c          566 g         1819 t
ORIGIN      
        1 gcattctcat tagagaacac tccctttttt gtgacttttc tgaagctaga gtgaatactt
       61 tcaactgcat tagtagtgta catgattttt ctaatggcgc tgccataatc aaagagttgt
      121 tcaacatgag aaaagtttcg tttgcataca tctactgcac cagaataatg agaccaccga
      181 ttttgaaaac tgtcgaaggc agcgtgtgca gcgtttaaag aagaagcact gtagaatttt
      241 ttcatgtctc gacagacttc cttataatcc ttacttggga ggtatcgtaa cgcatttcga
      301 acaagatgaa caatacagcg ctgcacaatg acagaaggaa aaattgcttt tgcgccttct
      361 ttgagaccgg aaacaccgtc catcgaaatg aaaaaaacat cttctacacc acctactttc
      421 agttcatcaa atacttgcat ccagcgattt ttagattctg tttgattgag ccataggcct
      481 aaaatctcct tgtttccttt aagatcatag ccaagaatgg tgtacacagc atattcttta
      541 gcttcatagt tttcgcgtaa agtgacgtac atacaatcaa cgaatagaaa tgcataacac
      601 ttggctaatg gccgagcttg ccattcttcc aattcaggaa ggacggcatc cgtgatatca
      661 gaaatcattt cgtgggaaat atcaaagccg taaatatctt caacggttgc ggcgatatcg
      721 cattgactca ttcctcgtgc atacatggaa agaaccttac cttcgatgtc ggagacatct
      781 cgttttctat taggaatcaa ctcaggttca aaagaagcct cccggtctct gggaacatca
      841 atgtttactt taccaaagct cgttttaagc gtcttattgc cataaccgtt tcgtcgattg
      901 tcatgtttct ttggctcttt agaatgggcg tcatacccta aatggttatt caattctcct
      961 tgaagcattt tttcaaataa gggaccaaac acatctttta aaacatcttg catatcctct
     1021 acagattcgg gttgataagt attcaaaatg gattcagcta acttctcagc atcaggattt
     1081 cttttctttc tagccatcgt gtaatcacct cttgatctta ttttagaaaa ataaaaaccg
     1141 tgaagcaacc acccgcctag gattgatggt tacttacacg gtttatatta cactctcatt
     1201 tttggtgcta aaaagcaccg aaaattttaa ttattgtcaa ttaataattc ttttggattc
     1261 cttcttaata tattgaatga agaaataatc aatgatacaa gaagtactaa actcataaat
     1321 ataactacat aaatcatgaa tttaggtaat acattgatat ctaaacttga taatgtcttg
     1381 ttaaatcctt cagcttctgc cccaccacct aattggctag atgcagattg tttagctatt
     1441 tgtttagcta tatcaccagt aaccttattc aatatattat tcccaagctt gtcagctgta
     1501 tattgagcta agaaatagga accaacgaat gcggggattg atataaatac catctcaatt
     1561 aggaattgac caaaaatttc taattttgat atacctaatg atagtaatac tgctatttct
     1621 ttctttctag cattcatcca taagaataat aataatgaaa ctacaactcc tgcaaatatt
     1681 aatgagccaa aaaataattt atttgaaatt gagtaaatac ctgatataga ttgttgtaat
     1741 gcagggtaat ttgatgaact tttaatcaaa ttatattctc tccaatttat atctaatttc
     1801 ccaagatctt ttattacact atcaagattc ttatcacctt ttacaaagaa tgttgcatct
     1861 tggtatacag ctgtatcttc ggtattacca taaactttag cagctgtatg aatatcagtg
     1921 attaatgtat tttcgtatag ttcttgtgct gcacttactc caccgctatt atgcccatcg
     1981 aatagacctt taatttctac ttctactgtt tcatctgcac ctttctcatt atcagcatca
     2041 aataaattag attttatttt tatcttatct ccaactttaa ggttattttt ttcagctaaa
     2101 tctttatgca ttaagatttt atttttatct tcattttcta aatgctttcc ttctactaat
     2161 ttatatgctt ctgatacaaa ttttgtttct tttgttgagt cgttaacccc agttaacata
     2221 actgttcttt taaaattctt tgctctttca ggtgattgat tcgcaagagt ctcttgagtt
     2281 tcaataatat cataatcaac taaatctgca acactgttta ttctttttac ataggagtct
     2341 atactatctg tttgagatat ctttttaata tcttcacctt ttacatttcc cccacctcta
     2401 ggtgttcctg gatttacttg tctatttatc tccatagaaa aactatttgt tatattagca
     2461 aatgtttctt ttgaagctct gtccgtagca tctttaatag ataaactaat gagacttaaa
     2521 gttgacatag ataaaataac taatatgata attaatgatt ttaaactttt tctagttaca
     2581 taagcaaatg cattttttat cattattcaa cctccaaatt cattttattt acttttttca
     2641 actttttacc acttagttct aaaatgatat ccgcagaatc tgctacttcc ttactatgtg
     2701 ttacaactat tacacattta tttctatctt tagctaatgt ctttaatata ttaattattt
     2761 ctccagcagt aacactgtct agattaccag taggctcatc agctagtatt attggagcat
     2821 ctgataccaa tgctctagca atagctactc tttgttgctg tccaccagat aatttcataa
     2881 catttctttt tatttgtttt ttatctaaac ctagttcaaa caagatactt tcatctgctg
     2941 atttatttac taatctaata ttttcaattg gtgataaata atctattaaa ttatagtttt
     3001 gaaatactaa agatatatta ttttttctat gattactata tcctttattt tgtatatctt
     3061 cattcttaaa caatattttt cctgtttgag gtttatcaag tccagcaagt aaggaaagaa
     3121 gtgtagattt tcctgttcct gactttccta ctatcgcata aaacttccca agttcaaatt
     3181 tttgatttac tcctgacaaa actttttctt tagaatttgc ataactatat gctacattct
     3241 ttatttctaa tatatccatt attttctcct aacttatttt tgataagatt tctttaggct
     3301 tcttgattaa cattaatgaa gaagcaaata caactgataa aacaataata cttattaata
     3361 ttaaataact ttgtccaagt gttgttatat ttgacataaa actactatta tttattaaac
     3421 ttccaccgga aatcattgaa tcctctgagt taatgaatcc atctacaatt acttttagta
     3481 atacatttcc taaaaataag gaagatatta tacttggtat cgatatgaat attaactcga
     3541 atataaattg cattataatt tgtatcttag atgttccaat agataagaat atacctattt
     3601 cataaattct ttcccttaac catagaatca agattaatga aagaacaacc atcccaccta
     3661 acataataga ataagtcatt acttttatta tatattttat tccactcact gactctaaag
     3721 actcttcaaa cgcattagaa tctttttcaa caaaatactt tgactcatca attttaagct
     3781 ctttcaattt gtttaaggct aagtctgtag attctgcact accagaatac attaaaattt
     3841 tatttgcaat ttcattattt tctgaattat ttaatatctc ttggcttgtt gaataatcta
     3901 caaacaccat attttcacta aaatcagaag ataatcctgt atatgtttcc tgttttttac
     3961 cagaaaagat ccctataatt ttaaatttat gactttttat ttttccactt ttttcaatat
     4021 ctagtaattc aagatcaact tcatcaccta attttagatt gttttgttta gcaaattctt
     4081 catgaacaat aattgaattc ttatcatttt cttctatatt tttaccttct ttaattgtaa
     4141 atactccact actaaataaa atatttcttt tagtattatt tgtagcttcg agtgaaacaa
     4201 cattcttaaa ttcgtcagat aaatcttctc tatttattct ttgttctccg ctaactactt
     4261 tagcatcttt tagttttgct aatccatcat attgaattat tttttcttca atttctttta
     4321 atttttcaat atctttaaat tgattaacat taaaatattt accatctttt tttgttattg
     4381 atattgaaga attagaactt tcatataaag ccttttctat ttcattgctt gatttcatta
     4441 ttgttaaaca agaatacaag caagaaagaa ctatagttaa tatgataaaa attattaaag
     4501 ttctattttt ctttcttgta atatatgcta tagcattttt tatcactttc ctaactactc
     4561 ctctctattt tcttactcca taactaattg catccactgg acaagcattt ttacatcgtc
     4621 cacatctgat acactccaga tgattgcaat tctcaactga atctttattc atttggcaaa
     4681 cttttttaca ttttccacaa ttaatacact tatccttatc
//
LOCUS       DACDXO010000108         4711 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E23605_2017
            SAMN12639989-rid9881463.denovo.114, whole genome shotgun sequence.
ACCESSION   DACDXO010000108 DACDXO010000000
VERSION     DACDXO010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639989
            Sequence Read Archive: SRR10023389
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4711)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4711)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741914.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:42:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,972
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 33 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 17 of 73
            Pseudo Genes (multiple problems)  :: 10 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4711
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639989-rid9881463.denovo.114"
                     /isolate="E23605_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            105..356
                     /locus_tag="IWM35_002616"
                     /pseudo
     CDS             105..356
                     /locus_tag="IWM35_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            449..1243
                     /locus_tag="IWM35_002617"
                     /note="aph(3')-IIIa"
     CDS             449..1243
                     /locus_tag="IWM35_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HAP9920433.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            1653..1764
                     /locus_tag="IWM35_002618"
                     /pseudo
     CDS             1653..1764
                     /locus_tag="IWM35_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008787626.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
     gene            1785..1868
                     /locus_tag="IWM35_002619"
     CDS             1785..1868
                     /locus_tag="IWM35_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuator leader
                     peptide ErmL"
                     /protein_id="HAP9920434.1"
                     /translation="MLVFQMRYVDKTSTVLKQTKNSDYADK"
     gene            1993..2730
                     /gene="erm(B)"
                     /locus_tag="IWM35_002620"
     CDS             1993..2730
                     /gene="erm(B)"
                     /locus_tag="IWM35_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HAP9920435.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            complement(2993..3856)
                     /locus_tag="IWM35_002621"
     CDS             complement(2993..3856)
                     /locus_tag="IWM35_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin zeta"
                     /protein_id="HAP9920436.1"
                     /translation="MANIVNFTDKQFENRLNDNLEELIQGKKAVESPTAFLLGGQPGS
                     GKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELVKLYEKDVVKHVTPYSNRMTEAI
                     ISRLSDQGYNLVIEGTGRTTDVPIQTATMLQAKGYETKMYVMAVPKINSYLGTIERYE
                     TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETP
                     SISPKETLEKELNRKVSGKEIQPTLERIEQKMVLNKHQETPEFKAIQQKLESLQPPTP
                     PIPKTPKLPGI"
     gene            complement(3858..4130)
                     /locus_tag="IWM35_002622"
     CDS             complement(3858..4130)
                     /locus_tag="IWM35_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HAP9920437.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELNKNDSQLLEV
                     NLLNQLKLAKRVNLFDYSLEELQAVHEYWRSMNRYSKQVLNKEKVA"
     gene            complement(4147..4362)
                     /locus_tag="IWM35_002623"
     CDS             complement(4147..4362)
                     /locus_tag="IWM35_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000260238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HAP9920438.1"
                     /translation="MIVGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIET
                     AKNGGNVKEVMDQALEEYIRKYLPDKL"
     gene            complement(4454..>4711)
                     /locus_tag="IWM35_002624"
     CDS             complement(4454..>4711)
                     /locus_tag="IWM35_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015543629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partitioning protein ParA"
                     /protein_id="HAP9920439.1"
                     /translation="MIGFVPYLVDTDSATIKSNLEELYKQHKEDNLVFQNIIKRSNKV
                     STWSKNGITEHKGYDKKVLSMYKNVFFEMLERIIQLENEKE"
BASE COUNT         1446 a          796 c          865 g         1604 t
ORIGIN      
        1 tcttgggagt tttgagacta ctatatgcgt ttttttgttg atttaccaac atgtttagac
       61 attttcctct aggaaaaaat taactagcac cacgggttat cttttatata gaagatatcg
      121 ccgtatgtaa ggatttcagg gggcaaggca taggcagcgc gcttatcaat atatctatag
      181 aatgggcaaa gcataaaaac ttgcatggac taatgcttga aacccaggac aataacctta
      241 tagcttgtaa attctatcat aattgtggtt tcaaaatcgg ctccgtcgat actatgttat
      301 acgccaactt tgaaaacaac tttgaaaaag ctgttttctg gtatttaagg ttttagaatg
      361 caaggaacag tgaattggag ttcgtcttgt tataattagc ttcttggggt atctttaaat
      421 actgtagaaa agaggaagga aataataaat ggctaaaatg agaatatcac cggaattgaa
      481 aaaactgatc gaaaaatacc gctgcgtaaa agatacggaa ggaatgtctc ctgctaaggt
      541 atataagctg gtgggagaaa atgaaaacct atatttaaaa atgacggaca gccggtataa
      601 agggaccacc tatgatgtgg aacgggaaaa ggacatgatg ctatggctgg aaggaaagct
      661 gcctgttcca aaggtcctgc actttgaacg gcatgatggc tggagcaatc tgctcatgag
      721 tgaggccgat ggcgtccttt gctcggaaga gtatgaagat gaacaaagcc ctgaaaagat
      781 tatcgagctg tatgcggagt gcatcaggct ctttcactcc atcgacatat cggattgtcc
      841 ctatacgaat agcttagaca gccgcttagc cgaattggat tacttactga ataacgatct
      901 ggccgatgtg gattgcgaaa actgggaaga agacactcca tttaaagatc cgcgcgagct
      961 gtatgatttt ttaaagacgg aaaagcccga agaggaactt gtcttttccc acggcgacct
     1021 gggagacagc aacatctttg tgaaagatgg caaagtaagt ggctttattg atcttgggag
     1081 aagcggcagg gcggacaagt ggtatgacat tgccttctgc gtccggtcga tcagggagga
     1141 tatcggggaa gaacagtatg tcgagctatt ttttgactta ctggggatca agcctgattg
     1201 ggagaaaata aaatattata ttttactgga tgaattgttt tagtacctag atttagatgt
     1261 ctaaaaagct ttaactacaa gctttttaga catctaatct tttctgaagt acatccgcaa
     1321 ctgtccatac tctgatgttt tatatctttt ctaaaagttc gctagatagg ggtcccgagc
     1381 gcctacgagg aatttgtatc gataagaaat agatttaaaa atttcgctgt tattttgtac
     1441 atttaacttg acggtgacat ctctctattg tgagttatta gtggtacagt tttcaaccgt
     1501 tttaattata aaaaagtggt gcatttttaa attggcacaa acaggtaacg gttattgcag
     1561 gtgtatttct tatctatggg tttaacatgg attttatcat taaaatcatg agtattgtcc
     1621 gagagtgatt ggtcttgcgt atggttaacc ctaaagttat ggaaataaga cttagaagca
     1681 aacttaagag tgtgttgata gtgcattatc ttaaaatttt gtataatagg aattgaagtt
     1741 aaattagatg ctaaaaattt gtaattaaga aggagggatt cgtcatgttg gtattccaaa
     1801 tgcgttatgt agataaaaca tctactgttt tgaaacagac taaaaacagt gattacgcag
     1861 ataaataaat acgttagatt aattcctacc agtgactaat cttatgactt tttaaacaga
     1921 taactaaaat tacaaacaaa tcgtttaact tctgtatttg tttatagatg taatcacttc
     1981 aggagtgata acatgaacaa aaatataaaa tattctcaaa actttttaac gagtgaaaaa
     2041 gtactcaacc aaataataaa acaattgaat ttaaaagaaa ccgataccgt ttacgaaatt
     2101 ggaacaggta aagggcattt aacgacgaaa ctggctaaaa taagtaaaca ggtaacgtct
     2161 attgaattag acagtcatct attcaactta tcgtcagaaa aattaaaact gaatactcgt
     2221 gtcactttaa ttcaccaaga tattctacag tttcaattcc ctaacaaaca gaggtataaa
     2281 attgttggga atattcctta ccatttaagc acacaaatta ttaaaaaagt ggtttttgaa
     2341 agccgtgcgt ctgacatcta tctgattgtt gaagaaggat tctacaagcg taccttggat
     2401 attcaccgaa cactagggtt gctcttgcac actcaagtct cgattcagca attgcttaag
     2461 ctgccagcgg aatgctttca tcctaaacca aaagtaaaca gtgtcttaat aaaacttacc
     2521 cgccatacca cagatgttcc agataaatat tggaagctat atacgtactt tgtttcaaaa
     2581 tgggtcaatc gagaatatcg tcaactgttt actaaaaatc agtttcatca agcaatgaaa
     2641 cacgccaaag taaacaattt aagtaccgtt acttatgagc aagtattgtc tatttttaat
     2701 agttatctat tatttaacgg gaggaaataa ttctatgagt cgcttttgta aatttggaaa
     2761 gttacacgtt actaaaggga atgtagataa attattaggt atagggcacc tctaataact
     2821 accaattaat tcacctcaaa atgaaattag aaaaaaacag cgaaattttt aaatctattt
     2881 cttatcgata caaattcctc gtaggcgctc gggaccccaa catgaggaaa tgtttttata
     2941 aaaaaagagt agttaccgtt ttttggtaac tactcttttc cttttttaag gtttaaatac
     3001 ctggaagttt aggtgttttg ggtattggtg gtgtaggtgg ctgcaagctt tccaattttt
     3061 gttgaattgc tttaaattca ggtgtctctt ggtgtttatt tagaaccatt ttttgctcta
     3121 ttcgttctaa agtcggttga atttctttcc ctgatacttt acgattcaat tctttttcta
     3181 aggtttcttt tggactaata gaaggtgttt ccaaacttga atagagtttt actccttctc
     3241 tgttatatag ccttatatcg ctaaataagc ccgttttatg aagggtttct aaattggtcg
     3301 gtaagttttt gacaacaata tcatgcgctt gttttggtgt tgccctggct gtcattggat
     3361 catctgcata catggtttca tatcgttcaa ttgttcctaa atatgagttg attttaggta
     3421 ctgccatgac atacattttt gtttcataac ctttggcttg aagcattgtt gcggtttgaa
     3481 taggaacgtc tgttgttcgt cctgtacctt cgatcaccaa attataccct tgatcgctca
     3541 aacggcttat gatcgcttct gtcatgcgat tagaataagg ggtaacgtgt tttactacgt
     3601 ctttttcata aagtttcact agttcatcaa aattagggtg ctgttgttta aaggtatcat
     3661 tatcaatgac aataacattc ccttgtgttt cttcaaaaat tgctgatcgc aaactggttt
     3721 tccctgaccc tggttgccca ccaagtaaaa aagcggttgg cgattcaacc gctttttttc
     3781 cttgaatcaa ttcttctaaa ttatcattta agcgattctc aaattgtttg tcagtaaaat
     3841 tgactatatt tgccatatta agccactttc tctttattca aaacttgttt tgagtaacga
     3901 ttcattgacc gccaatactc atgaacggct tgtaattctt ctaaagaata atcaaaaaga
     3961 tttacacgtt ttgcaagctt taattggttt aataaattga cttccaataa ttgagagtca
     4021 tttttattta attcatggtt caaaacatag tttaatactc gattataaac gcttgccgat
     4081 aattcgttaa tgatctctat ttcaaatgtt ttttcatacg taactgccat ttcattttca
     4141 ctccttttaa agtttgtcag gtaaatattt ccgtatatat tcttctaagg cttgatccat
     4201 aacttctttt acgtttccgc cattctttgc tgtttcgatt tttatgatat ggtgcaagtc
     4261 agcacgaaca cgaaccgtct tatctcccat tatatctttt tttgcactga ttggtgtatc
     4321 atttcgtttt gctttttgtg cgcctaaatt tcccacaatc actcacttct ttctatttct
     4381 tcttactctt attttatcat caacaatcac aaatcacaag tgatttgtga ttaatcactt
     4441 gtgatttgtg attctattct ttttcgtttt ctaattgaat aattcgctca agcatttcaa
     4501 aaaatacgtt cttatacatg gataaaactt ttttgtcata gcctttatgc tctgtaatgc
     4561 cgtttttaga ccaggtactt actttattac ttcgcttgat aatattttgg aaaaccaagt
     4621 tatcttcttt atgttgctta tacagttctt ccaggtttga ttttatcgtt gcgctgtccg
     4681 tatcaactaa ataaggaaca aaaccgatca t
//
LOCUS       DACDXO010000109         4650 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E23605_2017
            SAMN12639989-rid9881463.denovo.115, whole genome shotgun sequence.
ACCESSION   DACDXO010000109 DACDXO010000000
VERSION     DACDXO010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639989
            Sequence Read Archive: SRR10023389
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741914.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:42:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,972
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 33 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 17 of 73
            Pseudo Genes (multiple problems)  :: 10 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4650
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639989-rid9881463.denovo.115"
                     /isolate="E23605_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            162..392
                     /locus_tag="IWM35_002625"
     CDS             162..392
                     /locus_tag="IWM35_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HAP9920440.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            489..1790
                     /gene="murA"
                     /locus_tag="IWM35_002626"
     CDS             489..1790
                     /gene="murA"
                     /locus_tag="IWM35_002626"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HAP9920441.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNRPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            1803..1979
                     /locus_tag="IWM35_002627"
     CDS             1803..1979
                     /locus_tag="IWM35_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HAP9920442.1"
                     /translation="MSNQRYILLTLIKILVVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            2114..2410
                     /gene="yidD"
                     /locus_tag="IWM35_002628"
     CDS             2114..2410
                     /gene="yidD"
                     /locus_tag="IWM35_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HAP9920443.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFQLTRNKKDKKEPDYKKVVKNTSSTER"
     gene            2474..2941
                     /locus_tag="IWM35_002629"
     CDS             2474..2941
                     /locus_tag="IWM35_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9920444.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVAPTYQGKGIGGKLLETAFQKLSQLASSQTQIIIRTGSTS
                     SAALHLYQKMGYVEIGRVKDYFIHHYSEPIFENGDQLRDQVILAIHLPKNSFN"
     gene            3026..3910
                     /locus_tag="IWM35_002630"
     CDS             3026..3910
                     /locus_tag="IWM35_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP9920445.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKAQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLN
                     GDKTFSKLGTAVQMAWRYCERKGLDKNSYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLVGAAANYLAVRQGEN"
     gene            4027..4260
                     /locus_tag="IWM35_002631"
     CDS             4027..4260
                     /locus_tag="IWM35_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HAP9920446.1"
                     /translation="MSKKNKDVEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYGP
                     KEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
     gene            4348..4428
                     /locus_tag="IWM35_002632"
     tRNA            4348..4428
                     /locus_tag="IWM35_002632"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4382..4384,aa:Tyr,seq:gta)
     gene            4435..4506
                     /locus_tag="IWM35_002633"
     tRNA            4435..4506
                     /locus_tag="IWM35_002633"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4467..4469,aa:Gln,seq:ttg)
BASE COUNT         1560 a          728 c         1016 g         1346 t
ORIGIN      
        1 ttttgtctca gtcccttttt ttgcatattt ttagcattta tttcctaaaa aatgtaaggg
       61 gattcctata tttttaagta tacttgtttt tccttttata cattttatgg tattatcatt
      121 ttattagtta ttataaaaca atcgttagaa aggacgtcat tatgcaagta tacggaattg
      181 atgcggtgct tcgaatcgtg agtcatttgg cgtttatcta cttagccttc tggtctttac
      241 gttcgttacg cattgaaact tttttcagac caatgcataa tacgcaaatt agaatggcaa
      301 tcgtactttt ttcaattttt ttagggtaca ctgcaagtaa ttttttctta gaagttatcg
      361 ctttatgccg aaatcttttc gttagcctgt aaattttaaa aaaaggtact attttaaaag
      421 caaggatgtt ataatagaaa agattttgag taagtgtatg ctcattggaa ttttttggag
      481 ggaacaacat ggaagagatc atcgtaagag gtggcaatca attaaatgga acagtaagaa
      541 ttgaaggagc taaaaatgct gttttgccta ttttagctgc tagtttgtta gcagaagaag
      601 gtattacgac attagacaac gttccgattc tttctgatgt cttcactatg aatcaagtga
      661 ttcgtcactt aaacgtagat gtagattttg atgaacaaaa gaatcaagta acaatcgatg
      721 cttcaagaca attagaaatc gaagcgcctt atgagtatgt tagtcaaatg cgtgcttcaa
      781 tcgttgtgat gggtccgcta cttgccagaa atggacatgc aaaagtagct atgcctggtg
      841 gatgtgcgat tggtaaacga ccaatcgatt tgcatttaaa aggatttcaa gctttaggag
      901 caaaaattat tcaaaaaaac ggctatatcg aagcaattgc ggatgaattg atcggtaaca
      961 cgatttattt agatttccct agtgtaggtg ctactcaaaa tatcatgatg gctgccgtaa
     1021 aagcaaaagg aacaaccatc attgaaaacg tagctcgcga accagaaatc gttgatttag
     1081 caaacatttt gaataaaatg ggcgctcaag tctatggagc tggtactgaa acaatgcgta
     1141 ttgaaggcgt ggatcattta catgcagtca accactcaat cgtacaagat cgtatcgaag
     1201 caggtacctt tatggttgct gctgcaatga cacaaggcaa tgtgctgatt gcagatgcta
     1261 tctctgaaca taaccgccca ttgatttcta aattgatcga aatgggtgca gaaatcattg
     1321 aagaagaagg cggagtccgt gtcatcggac caaaacatat cttacctaca gatgtaaaaa
     1381 cgatgcctca tcctggtttc cctacagata tgcaagctca aatgacagct atccaattag
     1441 ttgcagaagg tacaagtgta gtgactgaaa cagtgtttga aaatcgtttc caacatttag
     1501 aagagatgcg ccgtatgaac gctcatgtca aaatcgacgg aaatgtagca atcatggatg
     1561 gaaaccatga attgcaaggt gcagaagtat atgcaacaga cttgcgggca gcagcagcat
     1621 tagttttagc tggcttgaaa gcaaatggaa tcacacgtgt gcgtaacttg aattatctag
     1681 accgtggata ttataatttc catatcaaac tacagcaatt aggtgcagac gttgaacgtg
     1741 tagacatgga tcaaacttct gctgaaaaaa cagcacaaac gattgcttaa ggaagtggct
     1801 gaatgagtaa ccaacgatat atcttattaa ctttgataaa gattttagtg gtgatcttat
     1861 tgcttatcct tctttttgtg gccggaacga tgatcggata tggtgtcatc ggtggcggca
     1921 atccatttaa agtattccag ccaagtttgt ggattcatat tcgagacttt tttcattgat
     1981 aaataagaat cacagtggtt ctagtcgata tgaagtgagc catgaagcag aacatgacaa
     2041 aagaaaacag aagggaacgt tgacgttagt cgcgatccct ttttttagta aaagaaaaga
     2101 aggaatctaa ttgatgaaga agatattgat ttatttagtt agaggatatc agcgctttat
     2161 ttcgcctgca tttccaccta gctgccgtta ttacccaacc tgttccaatt atatgataaa
     2221 agcaattcaa acccatggtg cactaaaagg gacggtaatg gggactgcac ggatcttacg
     2281 ctgtcatcct tttgtcaaag gcggtatcga ttatgtacca ttgacattcc aattaacgcg
     2341 aaataagaaa gataaaaaag aaccagatta taaaaaggtt gtgaaaaata cgagtagcac
     2401 tgagcgataa agaaaaagag gaaaattatt gtcaaagtaa caactccgca actacttata
     2461 tgggggacaa aaaatgatcg aagaactaaa caagaaagat ttaccatggt ccttgttatt
     2521 agatgcagat cctgataaag aaaaagttca agtatatgtt tccagaggga gtggctggat
     2581 atggaaagaa aaagaaaaaa caataggtgt actgatctat gtggcaaggg aaaaagaata
     2641 cgaaatcgtc aatgtagcag tagccccaac gtatcaagga aaggggattg gcggaaaatt
     2701 acttgagaca gcttttcaga agctttcaca actcgcttcc agtcaaactc agatcatcat
     2761 acgaaccggc agtactagca gtgcggcgtt acatctctat caaaaaatgg gctatgtgga
     2821 gattggtcga gtcaaagact attttatcca tcattactct gagcccattt ttgaaaatgg
     2881 agatcaatta agggatcaag taattttagc aattcactta cctaaaaata gctttaacta
     2941 ataaactttc aattcgagaa aatgctttca aatgctaaat agatacatta caatagcttt
     3001 agcaaacaaa aaggagaata tttttatggg aatattagtt tttgattttg gtggatcggc
     3061 tgtcaaatat ggttgttggg atggcaaaga gattaaaggt aagggacaat ttgcaacgcc
     3121 tgaatcttgg gaagaaatga aagcgcaatt atttcaagtt tataaaaaaa tactcgtttc
     3181 ttttgaagtc gaaggaattg gaatcagctc tcctggagta gtgaataatg agaaacaggt
     3241 gattgaggga atcagtgcga ttccttatat tcatggattt aatattttcc gagatttgga
     3301 aactctgttt caactcccag taacgattga aaatgacgcc aactgtgccg gaatggctga
     3361 attttatgaa ggtgctgccc gtgattatca agatgttgct tttgttgtgg caggaactgg
     3421 gattggcggg gcgttgttca cgaaaggaca aatcaataaa ggggcacatc tttatggtgg
     3481 agaatttggg ttgatgttct taaatggcga taagacattc agtaaacttg gtacagcggt
     3541 acagatggct tggcgttatt gtgaacgcaa aggtctcgat aaaaattctt ataccggaaa
     3601 agatgtcttt gaattagcag aaactggaga tgcaatagca aaagaagaag ttgagagttt
     3661 ttatacgtat cttacaaagg gattattcag tattcaattt tcgtttgatc cagaagtgat
     3721 cgtattaggc ggcggtgttt ctgcaaaaga agggctcatt gatgagatca aaagccgaat
     3781 gaagaagctg acagaacaat tcgatctgca tgatttcgaa ccgcagattt tattatgtga
     3841 atacagaaac gatgctaatc ttgtaggtgc agcagcaaac tatctcgctg tacgtcaagg
     3901 ggagaattaa gagctttaga gaaggtgcag cttgcataaa agaagtgaat gaaatgaata
     3961 ggagattttt acctaaggaa tgttttattt gctgcagtat gataaaatat ctgagaggtg
     4021 aaaatgatgt caaaaaaaaa taaagatgtt gaagtgcgta tagaagaagt gaaaaaagat
     4081 atcaaaggta agaattatga agtcactcag ttatttatag ggaaaaaaat gattggtgag
     4141 atcttagcct atgggccaaa agaatatgaa attttctttg gtgaagaaga ttttggaaaa
     4201 gaaaaaagtc ttgaaaatgc gatagaaaca gtgattcgac actggaatct gcacgaataa
     4261 aaaaatatca aaaaaacttt caataaaggg ttgcaaaaat ctggaaaatt aggtattata
     4321 actaagtcag gttgatacaa acctttcgga ggagtagcga agtggctaaa cgcgacggac
     4381 tgtaaatccg ttccttaggg ttcagtggtt cgaatccact ctcctccatt tcattggggt
     4441 atagccaagc ggtaaggcaa gggactttga ctccctcatg cgttggttcg aatccagcta
     4501 ccccagtata ccacaatcgt aagctctcgt tagttcgaga gctctttttt ttgctttgct
     4561 accttgttcc ttgaagattc tgctgattta agatagagtt aagttaacta caagaattgg
     4621 agggatccac atgaaatgga aagaaaaagt
//
LOCUS       DACDXO010000110         4632 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E23605_2017
            SAMN12639989-rid9881463.denovo.116, whole genome shotgun sequence.
ACCESSION   DACDXO010000110 DACDXO010000000
VERSION     DACDXO010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639989
            Sequence Read Archive: SRR10023389
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4632)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4632)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741914.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:42:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,972
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 33 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 17 of 73
            Pseudo Genes (multiple problems)  :: 10 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4632
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639989-rid9881463.denovo.116"
                     /isolate="E23605_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            174..518
                     /locus_tag="IWM35_002634"
     CDS             174..518
                     /locus_tag="IWM35_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HAP9920447.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVGWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            519..938
                     /locus_tag="IWM35_002635"
     CDS             519..938
                     /locus_tag="IWM35_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HAP9920448.1"
                     /translation="MTTKHEEIHNGVIFPAGEKNEAYAQVFTGQSYLNMLVNDPEVSV
                     GVGNVIFEPGCRNHWHVHKDGYQILLVTGGEGWYQEEGKPARRLMAGDVVVTHKGIKH
                     WHGATKDSWFEHLAITAGNSEFLEPVTDEVYEGLENQ"
     gene            complement(1030..1380)
                     /locus_tag="IWM35_002636"
     CDS             complement(1030..1380)
                     /locus_tag="IWM35_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9920449.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(1346..2566)
                     /locus_tag="IWM35_002637"
     CDS             complement(1346..2566)
                     /locus_tag="IWM35_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HAP9920450.1"
                     /translation="MLSNTPTDNWSGLLTILTSPSNLLTDYFALGGFGSAFINVGILT
                     LLSVLLAYRHKVILNGPLFASILTVTGFSFFGKNLYNSISIILGVYLYAVFVNKPFSQ
                     YIMIGLFGSALSPVVSYITFGMRFPLLIGILLGNLAGIAIGLLLPPLAAQTLVFHRGF
                     TLYNIGFTSGLIAMTFTAVLRLFSYSIVENTLVFNEYHFPLIWIIFGFFSLTVGIGFY
                     YNSFRLLGIREIFDSSGKLTTDFIANSGIGATLINIGLVGLMLSSYVLLVGGQLNGPV
                     IGAILSAVGFSAFGCHLKNSFPILVGIFIASLFGTFHEITSTGMLVAAVFGTGLAPIS
                     GFYGSFYGVIAGVLHIALVHNVSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKE
                     KKTLGKRIIKKNHR"
     gene            2932..3498
                     /locus_tag="IWM35_002638"
     CDS             2932..3498
                     /locus_tag="IWM35_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP9920451.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            3643..3730
                     /locus_tag="IWM35_002639"
     tRNA            3643..3730
                     /locus_tag="IWM35_002639"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3675..3677,aa:Ser,seq:cga)
     gene            4064..>4632
                     /locus_tag="IWM35_002640"
     CDS             4064..>4632
                     /locus_tag="IWM35_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HAP9920452.1"
                     /translation="MCTAITYATKDHYFGRNFDYEMSYNEVVTITPRNYRFDFRKVKN
                     LDKHYAMIGIAAGVSNYPLYYEATNEKGLSMAGLNFPGNADYKELQEGKDNVAPFEFI
                     PWILGQCSTIDEAKELLATINLVNIDFSEKLPLSPLHWLLADKEKSIVIESMKDGLHL
                     YDNPVGVLTNNPPFDYQLFNLNNYRSLSNG"
BASE COUNT         1682 a          778 c          891 g         1281 t
ORIGIN      
        1 aaataaaaaa agcgttagca actatgctta aaaagcatat cgactgaata aactaagcat
       61 tggacaggct gaaagactac atttaaaatg aaatgcgaag aaacaataga aaataaagtt
      121 gccttagaac catagttgaa tcacctaaca gtcgtgaagg gagaataaga gaaatggcag
      181 aaaaacaaac cgcaggacga gatcaattag gagattttgc accaaaattt gcagaattga
      241 atgatgatgt tttatttgga caagtatggt ccagagaaga attatctgct catcagcgta
      301 gtatcatcac catatctgca ttaatttcag gaggaaattt tgaacaattg ccagctcatc
      361 tgaagattgg caagcaaaat ggcattacta gtgaagaaat agcagaaatc attacccatt
      421 tagcttttta tgtaggatgg ccaaaagctt ggtcagcttt tagtttagca aaagaaatct
      481 atttagaaga aaatagcgaa gataagaaag aggcgtaaat gacgacaaaa cacgaagaaa
      541 tacacaatgg cgtgattttt ccagcaggtg aaaaaaacga agcttacgct caagtattta
      601 ccgggcaaag ctatctgaat atgttagtga atgatcccga agtttctgtt ggtgtaggta
      661 atgtaatttt tgaaccaggt tgtcgcaatc actggcatgt ccataaagac ggctatcaaa
      721 tcttacttgt gaccggagga gaaggctggt atcaggaaga aggaaagcca gcaagacgat
      781 taatggctgg agatgtcgtc gtaacgcaca aagggattaa acattggcat ggtgcgacaa
      841 aagacagttg gtttgaacat ttggcgatca ctgctggtaa ttcagagttt ttagaacctg
      901 taacagatga agtgtatgaa ggattggaaa atcaataatg tcaagcttga acagctcctg
      961 aaagtagatt ttaatcgaac tttcaggagc tgcttttttg attcagtctt ttataaatag
     1021 aaaatagggt taataatagg aatgagaagc acgtcgaaag atcttaatat tgataatcgg
     1081 ctgggcatac atgatttcca cgtcatcaat caaactacct aacagatgat aatcttcccg
     1141 atcatgtcgg ggattaccta atagttcttg atagtagatt tccatactag ggtcccgagg
     1201 attgttcagc aaatcagcaa gtacatctaa atcttctggc tgagtttctg ccgtaccctg
     1261 tatctctatg aaaagccctt cctcagcaaa ttctactcca atacgtattt cagttatttc
     1321 atgggaatat aaataagtaa gaagctcatc gatgattctt tttgataatt ctttttccaa
     1381 gtgtcttttt ttcctttctg acagcggtaa agttatctag tatcggtaca aggatacctg
     1441 caacgaaacc agttgaaaaa ccactgttat atagatttag tcctccgtgc aaagtgctga
     1501 cgttatggac aagagcaata tgtagtacac cagctattac accgtaaaaa ctgccgtaaa
     1561 aaccgcttat gggcgcgaga cctgtcccaa acacagctgc aacaagcatg ccagtactgg
     1621 tgatctcatg aaaagttcca aacaaagaag caataaatat tccgactagt atcggaaaac
     1681 tgttttttaa atgacaacca aaagcactga aacccaccgc agataaaata gcaccgatga
     1741 caggtccatt taactgaccg ccaaccagca aaacataaga agacagcatc agaccaacta
     1801 agcctatgtt gatcaaagta gcaccaatac cagaattagc aatgaaatca gttgtcaatt
     1861 ttccagatga atcgaaaatt tctcgaatcc ccaaaagccg aaaggaatta taatagaaac
     1921 caattccgac tgtcagcgaa aaaaagccaa aaataatcca aatgagagga aagtgatatt
     1981 cattgaaaac caatgtattt tcgacgattg aatagctgaa taacctcaaa acagcagtaa
     2041 aagtcatcgc aatcaaccca gaggtgaatc caatattata aagtgtgaat ccacgatgga
     2101 aaactaaagt ttgagcagct aagggcggaa gaagtagacc aattgctatg ccagctaaat
     2161 tccccaataa aatacctatc agtaaaggaa aacgcatacc aaaagtgata taactaacga
     2221 ccggtgataa tgcgctacca aacagtccga tcatgatgta ctgagaaaaa ggtttattga
     2281 caaatacagc gtatagatac acacctaaaa tgattgagat agaattataa aggtttttgc
     2341 caaaaaaaga gaaaccggta actgttagaa tcgaagcaaa taagggaccg tttaaaatca
     2401 ccttgtgccg ataagctaaa agcacagaaa gtaaggtcaa aatccctaca tttatgaatg
     2461 cgcttccgaa accgcctaaa gcaaaataat ctgtcaaaag attgcttggc gaggtaagaa
     2521 tagttagcaa tccactccaa ttatcagtag gagtattaga aagcaagcca ataaaaagaa
     2581 aaaaacacct gtaaaaccaa gataaaaata ctttcttttc tgtgaaacga ttcgtttttg
     2641 aggatgaata taaataacgt cagtttcgtt ttccattaca caccttcttt ttcattttct
     2701 ttaactataa cttgaaatga aagatttgga caactatcat ttaggtcaaa actaaaaatg
     2761 taagggaacc cattgaattt aaccaaattt ttttaatttt gcaaaaaaaa gttaaagaag
     2821 actgaaaaaa aatttaaaat ataacgtttt gagtaaaatg tttgcaaaaa atgctaaaaa
     2881 tagggtacaa taaaacaaca tatactgcag taaagaagtg ggggagtttt aatgagaaga
     2941 aaagtctata ccaaggatca aattctaaaa gctgcttatg atgttgttgc aaaggaaggg
     3001 tttaaaggat ttactgcgag aaatattgct aaaaagatgg ggatttcgac acagccgatt
     3061 tatctggaat tcaaaaacat ggaagattta aaaaatacgt tgctggattc tatctttgaa
     3121 caactaaaag aagatgtttt ccctattgaa cgtactggtg ataaaatcat tgatctcggt
     3181 ttgaactata tctactttgc aaaaaagcat catagtcttt acatggcatt gtacattgat
     3241 gaacatgggg acggaaagaa aatgcatgat ttttcataca attatttccg tgaaatagtt
     3301 aaagaagatg aaagatacaa tcaattatcg gatgaatatg tcgatgctct gcatactggt
     3361 acatgggtag cagtgactgg cttagcgtca ttgatgtcat caaacatcat gcatccaaca
     3421 gaagaacaaa tcattgcact gatcaaacgg acaattgact cgattttagc attagataat
     3481 cctgaaataa tcagatagtg ttctatcagt atgaatttga tcggtaagtc cgttggctgc
     3541 aaactgtcaa tggacttttt tgctaaaaaa agcgatttct gttatttatc tattagaaat
     3601 aggcttgaca tttcgtgaaa aaagtttaaa aatagtactt acggaaggtt ggcagagtgg
     3661 taatgcaccg gactcgaaat ccggcgaacc ggctaatacc ggcgcgcagg ttcaaatcct
     3721 gtaccttcct tatatcaacg attagaggtt gtgaagaagc tgtcctgcat caagtaataa
     3781 gaacggccaa acaaaaatag cttttcctat tttttcgttt gattgggctt attaccgcag
     3841 atgcaagctt ttgaacaccg tttgttcgga ataaggggct gtgacaagac ttttgtcaca
     3901 gccccttatt gttgtacttt taacctacaa tattttttga ttccccctga gtactatttt
     3961 ttataaatgc tagtaaatca atcttctttg gccttttttt gataaaaaaa atactagatg
     4021 taagataaaa gtgtaaaaac gatttgtttt aggaggggaa acgatgtgta ccgcaatcac
     4081 ttatgcaacg aaagaccatt attttggaag gaattttgat tatgagatgt cttataatga
     4141 agtggtcact attactccaa gaaattatcg tttcgatttt agaaaggtaa aaaatttaga
     4201 taaacattat gcaatgatag gaatagcagc tggggtatcc aactatccac tctattatga
     4261 ggctacaaat gaaaaagggt tgagtatggc aggactgaat tttcctggca atgctgatta
     4321 taaagaatta caagaaggaa aagataacgt tgcaccattt gagtttattc catggatact
     4381 agggcaatgc tcaaccatag acgaagctaa agaattattg gctactatca atctagtcaa
     4441 tatcgatttt agtgaaaaac tacccttgtc acctttacac tggttattag ctgataaaga
     4501 aaaatcgata gtaattgaaa gtatgaaaga tggacttcat ttatatgata atcctgtcgg
     4561 tgtgttgaca aataatccgc catttgatta tcaattattt aatctgaaca attatcgctc
     4621 gctatcaaat gg
//
LOCUS       DACDXO010000111         4611 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E23605_2017
            SAMN12639989-rid9881463.denovo.117, whole genome shotgun sequence.
ACCESSION   DACDXO010000111 DACDXO010000000
VERSION     DACDXO010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639989
            Sequence Read Archive: SRR10023389
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741914.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:42:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,972
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 33 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 17 of 73
            Pseudo Genes (multiple problems)  :: 10 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4611
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639989-rid9881463.denovo.117"
                     /isolate="E23605_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(86..340)
                     /locus_tag="IWM35_002641"
     CDS             complement(86..340)
                     /locus_tag="IWM35_002641"
                     /inference="COORDINATES: protein motif:HMM:NF024673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAP9920453.1"
                     /translation="MTATEIRNNKLKKKISTIFFTNKQRYGATKIHQVLLKEGISVSL
                     KHVQKLMKQLNLRSIVVKKYRPQRSNKPIMDRLQLTRQKN"
     gene            complement(451..705)
                     /locus_tag="IWM35_002642"
     CDS             complement(451..705)
                     /locus_tag="IWM35_002642"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP9920454.1"
                     /translation="MTRYEENFKQMIVELNQTGRSVRGLAKEYGLSEATIYKWKNLYL
                     PDQSTGLTGKEVAELRKENARLNEELEILKKAAAIFSRKT"
     gene            complement(898..1173)
                     /locus_tag="IWM35_002643"
     CDS             complement(898..1173)
                     /locus_tag="IWM35_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgO"
                     /protein_id="HAP9920455.1"
                     /translation="MALLPTNKDKKKIGAEDISIKDDINVELGERKYTAKERKPVQVD
                     PPVLKTIRDLSYAKDIPMYEIVKLAMDAYVDTLNDEERIIYNRRSKD"
     gene            complement(1145..2098)
                     /locus_tag="IWM35_002644"
     CDS             complement(1145..2098)
                     /locus_tag="IWM35_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAP9920456.1"
                     /translation="MENLRTAFKTSDKLGKVVMLTGRKGGIGKTTDNDLLAIVSSQLF
                     EKDVLLIDYDQQRNTTSNIGSTYQITSFDRSMSAAIKKGDWVSGITQVSPHLYIMAGS
                     PGSEELNEYLSEKYPDRRKRSLAFIKPLEELRKNFDYIFIDCPPSTDNVVRAFLTAAD
                     YIIAMQELKRYAMEGTEDFINKVLVPIVTNFEESHLQIIGILPVLFSVRRSSQHANYQ
                     KTIDKYGESNIFKSIVKGSDRLEMYGENGIQLNDYVDRRWWAIFADIFTELEERIEYY
                     EQHGDIEGFDYTPKYADSMANHILPLGKEIKLNGIITNEQR"
     gene            2710..4203
                     /locus_tag="IWM35_002645"
     CDS             2710..4203
                     /locus_tag="IWM35_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein RepR"
                     /protein_id="HAP9920457.1"
                     /translation="MLSEIYSAILKNGLRTYKYRNSTMKPAGHIDSSRSGAIFAYRSK
                     QLMNVGRGMVITSEEAILENEKKLTHWTPNTYRFGTYADDYRTVVKGHSEKNLSQINT
                     FVVDIDSKENHQGEIILACLDQVGYMPTLILESDHGYQVYFVLKTPGYVTKKSNFKVI
                     DVAKKISKTIRLQLAEKLSGVDLGCNHFGIARFPQENNIVFKEMNYQYSFADWIQWSM
                     KLESDQKTKEDRERKLIVFPEKKEIRQVDEPWFDMLLHKANIVGGEGRLGRNNVIFTL
                     SLAYYSSGYALDTCEYNMFTFNERLQEPLSEAELVKIVRSAYSGNYQAASREFIIELC
                     QQWVSPTLKNNDLFIQRRGWWKFKKPRDQRKYSHKNEWKEDLLAYLSEKSLASEPYLN
                     LSKKELASQLTMPKRTLDKILNELREENKVFYKVTRGRNGGMIIASVKTLFARVIQLS
                     KEKRLDYSTRITEVFGLNESLVEETFRQLSTPLNPLKEVNLFEVDTG"
     gene            4338..>4611
                     /locus_tag="IWM35_002646"
     CDS             4338..>4611
                     /locus_tag="IWM35_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HAP9920458.1"
                     /translation="MSLKNYVYSHPVNVFIIGRLGLSVEVFCELYGFKQGTLSSWVTR
                     EKTVASLPIEFLHGLSLASGETMDQVYDCLCVLEQEYIEYKIANELR"
BASE COUNT         1492 a          774 c          802 g         1543 t
ORIGIN      
        1 ttgcttgaat tcttgtgaaa aagttcttcg tgttctctta gacataaaaa ttcctcctgg
       61 tatgttttct tctagtctac acaccttaat ttttctgtct agttaattgt agcctatcca
      121 tgatcggttt attagacctt tgaggtctat attttttaac tacaatcgac cttaaattta
      181 attgtttcat tagcttttgg acatgtttaa gagatactga aatcccttct tttaataaaa
      241 cttgatggat ctttgtggca ccatagcgtt gtttattcgt aaaaaaaatt gttgaaatct
      301 tctttttcaa cttattattt ctgatttctg tagctgtcaa cggtttattc ttataaaaat
      361 aatagacact tctagggatt tctaataacc gacacaacaa agaaacagtg taatccttac
      421 accatttttc aataaattga acaagcgaat ttaggttttc cgagagaata tggctgcggc
      481 tttttttaag atttcaagtt cctcatttaa acgagcattt tcttttctca gttcagctac
      541 ttcttttcca gtcagtcctg tggactgatc aggtaaatat aaattcttcc atttgtaaat
      601 cgttgcttca gataagccat attctttcgc taaccctcga acagaacgtc cagtttgatt
      661 cagttcaaca atcatttgtt taaaattttc ttcatatcgg gtcataaaaa ttcatcctct
      721 cgtagataag tttaatttat tcttgtctct acgaaaagct gtctaagatt tgatactaac
      781 accaaatgtt gtataccccc aaacaataca catattttga aggattagga ttaataaagc
      841 taaaaatata gtaagtatac taaatatatt tactatatct agtatactta ctatatttta
      901 atcttttgat cgtctattat atataatccg ttcctcgtcg tttaaagtat caacataggc
      961 gtccattgca agtttgacta tttcatacat aggaatatct tttgcataac taagatcccg
     1021 aatggttttt agtacgggag gatctacttg aactggtttt ctttctttcg cagtgtactt
     1081 tcgttcccct aactcgacat taatatcatc tttaattgaa atatcttcag caccaatttt
     1141 cttcttatct ttgttcgttg gtaataatgc catttagttt aatctccttt ccaagaggta
     1201 ggatatgatt ggccattgag tcagcatatt taggtgtata atcaaatcct tcaatatcac
     1261 catgttgttc atagtattca attctttctt ctaattctgt aaagatgtct gcaaaaatag
     1321 cccaccatct tcgatctaca taatcattta attggatacc attctcacca tacatttcta
     1381 accgatccga gcctttaaca atgcttttaa aaatgtttga ttcaccatac ttatcaattg
     1441 ttttttgata gtttgcgtgt tgagaggaac gtcgcactga gaaaagaaca ggcaaaattc
     1501 caatgatttg aagatgagac tcttcaaaat tcgtgacaat cggaactaaa actttattaa
     1561 taaaatcttc tgttccttcc attgcgtacc gttttaattc ttgcattgca ataatataat
     1621 ctgcagcagt taagaatgca cgaaccacat tatctgttga tggtgggcag tcgataaaga
     1681 tataatcgaa attttttctc aattcttcta aaggtttaat gaaggccaat gatctcttac
     1741 gacgatcagg atatttttcg gacaagtatt cattaagttc ttcagatcca ggagagccag
     1801 ccataatata taaatgtgga cttacttgag taatacctga aacccagtcc ccttttttga
     1861 ttgctgcaga catggatcga tcaaagctag taatttgata cgttgatcca atgttggaag
     1921 tagtatttct ttgttggtca taatcaatta gcaacacgtc tttttcaaaa agttgactag
     1981 aaacaatcgc taataaatca ttgtctgtag tcttaccaat gccgcctttt ctacctgtaa
     2041 gcattacaac ttttcctagt ttatctgatg ttttaaatgc agtccgtaag ttttccatag
     2101 ttccctccaa tttcctatac taaatatatt tagtatactt catatactta atatattcga
     2161 tatatctaat atactccaaa ttgataagaa ttcagacttt ataaggaata tagtggatat
     2221 actaaatata tttgctatat tcagtatatc agatatattc aaaatgtcaa aatatatttg
     2281 tgcgttttgc gttgacaatt tagagtgaaa agaggtaaac ttaattctat agaaaccaca
     2341 gataattcac tatttacaaa gtagcaaaag cagtggtact ctttaaacag aaaaattaca
     2401 taaaaaaaag agacggaaat aattgatttt agcagaatca attatttctg aatcacatgg
     2461 ttaggacatg tgttcacgct cgtctcaaga atatgtaagt agctaccaac tacttatatt
     2521 aaaagttgat cggatacctt ttccgattgc ctttattcta ccaaaaaaag gcgagcaact
     2581 caacagaaga acgtttattt agtgtactta aattaaatat tcttaaacac tgaagagaga
     2641 acacatacta tggctgagat taaagctaaa ctgtgttctc tttttgtttg agaaaggacg
     2701 ttacaatcga tgttgagcga aatttattca gctattctta aaaacggctt acgtacatac
     2761 aaatatcgaa acagcaccat gaaacctgca ggtcatattg attccagtag gagtggtgct
     2821 atctttgcgt accgtagcaa gcaattaatg aatgttgggc gtggtatggt cattacttcg
     2881 gaagaagcta ttttagagaa cgaaaagaaa ctaacgcatt ggacacctaa tacctatcgc
     2941 tttggtactt atgcggatga ttatcgaaca gtggttaaag gtcattctga gaaaaactta
     3001 agtcaaatca acacatttgt agttgatatc gattcaaaag agaaccatca aggcgaaatt
     3061 attttagcct gtttagatca agtgggctac atgccaacgc taattttaga atcagatcat
     3121 ggttatcaag tttattttgt actaaagaca ccaggctatg tcacaaagaa aagcaatttc
     3181 aaggtgatcg atgtagcaaa aaaaatttca aaaacaattc gtctacagtt ggctgaaaaa
     3241 ttgtccggag tggatctagg atgtaaccac ttcggaattg ctcgattccc acaagaaaat
     3301 aatattgttt ttaaagaaat gaactatcag tattcttttg ctgactggat tcaatggtcg
     3361 atgaagcttg aatcagatca aaaaacaaaa gaagatcgag aacgaaaact aattgtcttt
     3421 cctgagaaaa aagaaattcg tcaagtagat gaaccgtggt tcgatatgtt acttcataaa
     3481 gcaaacatcg tcggcggaga aggacgtctt ggtcgaaata atgtgatttt cacgctttct
     3541 ttagcttatt actcttcagg ttatgcattg gatacttgcg aatacaatat gttcacattc
     3601 aacgaacggt tacaggaacc gctatctgaa gcagaattag taaagattgt acgtagtgct
     3661 tattctggta attatcaagc agcaagcaga gaattcatta ttgaattgtg ccagcaatgg
     3721 gtttcaccaa ccttaaaaaa taatgatctg ttcattcaac gacgtggctg gtggaaattt
     3781 aaaaagccac gagatcaaag aaaatatagt cataaaaatg aatggaaaga agatctattg
     3841 gcttatctaa gcgaaaaaag tcttgctagt gagccttatt tgaacctgtc aaaaaaagaa
     3901 ttggcttcac aactaacgat gcccaagcga acacttgata agatcttaaa tgagttacga
     3961 gaggagaata aagtcttcta caaggtaaca cgaggaagaa atggcggaat gatcattgct
     4021 tcagtcaaaa cgttgttcgc gagggttatt caactaagta aagaaaagag actagattat
     4081 tcaacaagga tcacagaggt atttggactc aacgagagct tagtagaaga gacatttaga
     4141 caattatcaa cccccttgaa ccctcttaaa gaagtgaatc tttttgaagt agatacaggt
     4201 tgaaaaataa aaggtgcaaa ttgccttata caatatatct atgatacttc tgtaggtgtt
     4261 gctccctttg cctagctacg tgtaggtggt atacttataa ctgcgtatgc agtcttatgt
     4321 atcatggagg aagttgtatg tcgttaaaaa attatgtcta ttcacatcct gttaatgtgt
     4381 ttatcattgg tcgtttggga ttatctgtgg aagtgttctg tgagctttat ggttttaagc
     4441 aggggacttt gtcttcttgg gttaccaggg aaaaaacagt tgcttctttg ccgattgagt
     4501 ttcttcatgg gttgtcctta gcttctgggg agactatgga tcaggtatat gattgtttgt
     4561 gtgtgttaga gcaggagtat attgagtata aaatagctaa cgagttaaga a
//
LOCUS       DACDXO010000112         4606 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E23605_2017
            SAMN12639989-rid9881463.denovo.118, whole genome shotgun sequence.
ACCESSION   DACDXO010000112 DACDXO010000000
VERSION     DACDXO010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639989
            Sequence Read Archive: SRR10023389
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4606)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741914.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:42:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,972
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 33 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 17 of 73
            Pseudo Genes (multiple problems)  :: 10 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4606
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639989-rid9881463.denovo.118"
                     /isolate="E23605_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(93..677)
                     /locus_tag="IWM35_002647"
     CDS             complement(93..677)
                     /locus_tag="IWM35_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP9920459.1"
                     /translation="MKIGYARVSTGLQNLDLQEDSLNKFGCEKIFTDHMSGAKSNRPG
                     LEMAIDFVRSGDTLVVWRLDRLGRNMEDLISIVNRLNERGVSFHSIQENITMDKSSST
                     GQLMFHLFAAFAEFERNLILERSAAGRAAARARGRFGGRPEKLSEQDLELLKTLVDSG
                     TPIKTIAERWNVSRTTIYRYLAKMEEKNGKTNSH"
     gene            complement(888..1091)
                     /locus_tag="IWM35_002648"
     CDS             complement(888..1091)
                     /locus_tag="IWM35_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9920460.1"
                     /translation="MPEFVNTPNFYQDSDILSKINLPKEESALQVQRTLQETQKIQKS
                     ISNLEDSEEKRKKRDQKTYQKEK"
     gene            complement(1101..1523)
                     /locus_tag="IWM35_002649"
     CDS             complement(1101..1523)
                     /locus_tag="IWM35_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9920461.1"
                     /translation="MKNKFILSLGILTIGISVTGTTIPTFADSTNEPTTVVDNNQELT
                     PLDRDGIAIGNPTNDLTVTENYLIVSPLLALAASKTIRVTGTMIFDKVGWPSRPPQSR
                     NWKEKRNGYWYYGKLYLQSYKSAGNNWIATYSGTLTRK"
     gene            1761..2375
                     /locus_tag="IWM35_002650"
     CDS             1761..2375
                     /locus_tag="IWM35_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP9920462.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDYVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVKLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSADAKDPQ
                     RRLVYKSIVEDLKNGVAIAKIAKDYNVTRQTVYRIKKDSMVNDK"
     gene            complement(2540..3364)
                     /locus_tag="IWM35_002651"
     CDS             complement(2540..3364)
                     /locus_tag="IWM35_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9920463.1"
                     /translation="MIERLKDFMISVWDVAMLADESNYTSSIYRLEDSNWIEISLELK
                     TLPNSVNYIIRDITSEAQQLVNYMHGHESIPFSLILLKRSKNISNTRDRFISIITACE
                     IGVKEFYQQEKPDLTLIFENLQSPPVDKLLGSMFKGYFNIEFPKEIRKEIKNFIEKRN
                     KLVHSPQGSKPTIEECFECYMAVLKTFNFFYKINDSFMYRDFYEESIELTPTEPNKSQ
                     ITLSKELERKVESQELAIGISITNVSTYKELYEAKSQDNKDFVENIDKEPTNTVGD"
     gene            complement(4002..4259)
                     /locus_tag="IWM35_002652"
     CDS             complement(4002..4259)
                     /locus_tag="IWM35_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HAP9920464.1"
                     /translation="MIKAWSDDAWDDYLYWHEQGNKSNIKKINKLIKDIDRSPFAGLG
                     KPEPLKHDLSGKWSRRITDEHRLIYRVENETIFIYSAKDHY"
     gene            complement(4252..4521)
                     /locus_tag="IWM35_002653"
     CDS             complement(4252..4521)
                     /locus_tag="IWM35_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAP9920465.1"
                     /translation="MEAVAYSNFRQNLRSYMKQVNEDAETLIVTSKDVEDTVVVLSKR
                     DYDSMQETLRTLSNNYVMEKIRRGDEQFSKGAFKTHDLIEVESDD"
BASE COUNT         1513 a          811 c          623 g         1659 t
ORIGIN      
        1 ataagttatc agtagacact tacaaaaaat tctcatacta ttgtcacttt tctagataaa
       61 tggcaaaaat tttcagcatt aaatgacaaa aactaatgag aatttgtctt tccatttttc
      121 tcctccattt ttgccaaata acgataaatg gtggttcttg agacgttcca ccgttccgca
      181 atagtcttaa taggtgtacc gctatccact aaagtcttca gtaattccaa atcttgttca
      241 gacaattttt caggacgacc accaaaccgt cctcttgcac gagcagctgc tcgtcctgct
      301 gcggatcgtt ccaggatcaa attacgctca aattctgcaa atgccgcaaa caaatgaaac
      361 atgagctgtc ctgtagaact tgatttgtcc atcgtaatat tttcttgaat actatgaaaa
      421 ctcacaccac gctcattcag tcgattgacg attgaaatca aatcttccat atttctacct
      481 aaacgatcta agcgccaaac gacaagtgta tcgccagagc gaacaaaatc aatggccatt
      541 tctaatcctg gtcgattact ttttgcccca ctcatatgat cagtaaaaat tttttcacaa
      601 ccaaatttat ttaggctgtc ttcttgtaaa tccaaatttt gtaaaccagt agatacccgg
      661 gcgtatccta ttttcaaaaa aatcactcct tcattaattt tattgtaaca taactttatc
      721 catatagttt aactgaacat agatattgtt atgggtttat gaacatgaaa tgaattgttt
      781 ttttgaaaaa taaagcaaaa aaagaaagtg tccagaaatg ggtgatttta tgaacacttt
      841 caattagaaa aaaagaatgt cgactagaca ctcttttttt aaaatcatta tttttctttt
      901 tgatatgttt tttggtctct tttttttctt ttttcttcag aatcttctag attagaaatt
      961 gatttttgaa ttttttgtgt ctcctgtaaa gttcgctgaa cttgtaaagc tgattcttct
     1021 ttcggtaaat taattttact tagaatatcg ctatcttgat aaaaatttgg tgtgttcaca
     1081 aattctggca taaaaaatcc ctactttcta gttaaggttc ctgaataagt cgcaatccaa
     1141 ttgtttccag ctgatttata actttgtaaa tacaattttc catagtacca ataaccattt
     1201 cttttttctt tccaattccg tgattgagga ggtctagaag gccaaccaac tttatcaaaa
     1261 atcatcgttc ctgtcactcg tatcgtttta gaagctgcta gagcaagtaa tggagaaact
     1321 attaaataat tttcagtaac agttaagtcg ttagttgggt taccaatggc tattccgtcc
     1381 ctatctaaag gagttaattc ttggttatta tctactacag tagttggttc atttgtacta
     1441 tcagcaaatg taggaattgt agttccagtt acactaattc caatagttaa aatacctaaa
     1501 gataaaataa acttattttt cattgtctat cacctcgaaa tcaaaactaa gaaaacgttt
     1561 tcttaataaa tcttaacaga taaatcaagt tatatactag aaaattcata aaatatattt
     1621 ttaatgtcat aatcgtttaa attttcttga ttgacattta tttacaaaaa atattgccat
     1681 ttaaaaagaa aagtgacata tggcttcctc gtgtatgatt agggtgtatt ttaaatagtt
     1741 gcagaaagta ggtaaaagtt ttgagagttg catacatacg tgtctcatcg atcgatcaaa
     1801 atgaacaacg ccagattgaa gaaatgaaaa aattcggtgc agagagaatt ttcatcgaga
     1861 aacaatctgg cgcaacgatt actcatcgac ctgttttcca agaagcatta gattacgtcc
     1921 gagatcaaga tatttttatt gtggaagcta ttgatcgact tggaagaaat tatgacgaga
     1981 tcatcgcttc agtcaattac ttgaagaaga aaaatgtgaa gctgatcatt actagtttac
     2041 cgatcatggc tgaagcaatt ggcaatcctc tcctggataa attcattaag gatctgatca
     2101 tccaaatttt ggccatgatc gctgaacaag aacggactga atcgaaacgt cgccaggcac
     2161 aaggaattaa gatcgcaaaa gcaaacggcg tctacaaagg gcggcccaaa ttatacagtg
     2221 cagacgcaaa agatccccaa cgtcgccttg tttacaaaag catcgttgaa gatcttaaaa
     2281 atggtgttgc gattgctaag attgctaaag actacaatgt tacgagacaa accgtgtata
     2341 gaataaaaaa agacagcatg gtcaatgaca aatagttgat catgctgctt ttattatctt
     2401 ctcatcttta atttcctaca tataattact atctataaaa ttaagtctta ctcagaacag
     2461 ataaagcata aaattccaac aaaccatttt ttacttaact aaataaagag tctgttaata
     2521 taatcttcac ttatttacgt taatccccta ctgtatttgt tggttcctta tctatatttt
     2581 ctacaaaatc cttattatct tgtgatttag cttcgtataa ttctttgtat gtcgacacat
     2641 tggttatact aattcctata gctagttctt gagactctac ttttctctct agctcctttg
     2701 acaaagtgat ttgagattta tttggttccg ttggagtcaa ttctatagat tcttcataaa
     2761 aatctctata cataaagcta tcattaattt tataaaaaaa attaaatgtt tttagcactg
     2821 ccatataaca ttcaaaacat tcttctatag ttggtttact tccttgagga gaatgaacca
     2881 atttattacg tttttctatg aaatttttaa tttcctttct tatttctttc ggaaactcaa
     2941 tattaaagta ccctttaaac atgctcccta ataatttgtc tacaggggga gattgtaaat
     3001 tctcaaatat tagagttaaa tcaggtttct cttgttgata aaattccttt acccctattt
     3061 cacaggctgt aattatcgaa ataaacctat ctctagtatt tgatatattt ttacttcttt
     3121 taaggagtat aagagaaaag gggatacttt cgtgtccatg catgtaatta actaattgtt
     3181 gagcttcaga agttatatct ctaattatat agttaacgga attaggaagt gtctttaact
     3241 ctaatgaaat ttctatccaa ttactatctt ctaatctgta tatgctcgaa gtataatttg
     3301 attcgtcagc aagcatcgct acatcccaaa cactgatcat aaaatctttt aaacgttcaa
     3361 tcatttcagc aatattgcta aaatcagcta atttttcttt agaataaaaa aaacatttat
     3421 atcagaatca ataaattcta ttttttttat tactatatcg tcatcattat cttgaccatt
     3481 tatgtttatt gttccctgag tggggagtac ttctttcaaa ttcaagttaa atttatattc
     3541 cattacttct cctccaatat ataaaaatta cttaactgta tccaaattta atatgtaaat
     3601 aaatcattaa tttttagcta aattctatga gaagtttatt tttgttgttt ttgctaatta
     3661 actaaaacta gtacaaaaaa tttaaaatag attattaaac ctattgctta ttctattcag
     3721 tcatatatat aagcgtcatc ctttagtaac taacatccca cactacaata gtattataac
     3781 atatctattc aacttcaaat tgttgaaatt taagccattt atatttactt ttcctcaact
     3841 ttagaaatga ttttactaac cttatatccg taatacgcgt aacaaacaca ttcattaata
     3901 acaatcaaca ctaagctata ttagcttttt aaatgactag ggtaaataaa ataaggatca
     3961 aaaactttta agtttctgac cctttcctta cttccgattg gttaatagtg atcttttgca
     4021 gaataaataa atatcgtttc attttcaact ctatatatca gtctatgttc atctgtaatt
     4081 cttctggacc attttccaga taaatcatgc tttaatggct caggttttcc taatccagca
     4141 aagggggaac gatcgatatc ttttattaac ttgttaatct tttttatatt gcttttgttt
     4201 ccttgctcat gccaataaag ataatcatcc caagcatcat cagaccaagc cttaatcatc
     4261 agattcaacc tcgattaagt catgtgtttt aaatgcacct ttggagaatt gttcatctcc
     4321 tcgacgaatt ttttccatga cgtaattatt agaaagtgtt ctcaacgttt cttgcataga
     4381 atcataatct ctttttgata atacaacaac tgtatcttct acatctttac ttgttacaat
     4441 aagtgtttca gcatcctcat taacttgttt catataacta cgtaaatttt ggcggaaatt
     4501 tgaataagct actgcttcca ttcctttcac cccttattta tttctataaa acaattgtac
     4561 attatattgt acaattaagc aaatgattaa tattctaact tcttat
//
LOCUS       DACDXO010000113         4477 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E23605_2017
            SAMN12639989-rid9881463.denovo.119, whole genome shotgun sequence.
ACCESSION   DACDXO010000113 DACDXO010000000
VERSION     DACDXO010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639989
            Sequence Read Archive: SRR10023389
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4477)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741914.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:42:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,972
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 33 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 17 of 73
            Pseudo Genes (multiple problems)  :: 10 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4477
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639989-rid9881463.denovo.119"
                     /isolate="E23605_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            323..1054
                     /locus_tag="IWM35_002654"
     CDS             323..1054
                     /locus_tag="IWM35_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAP9920466.1"
                     /translation="MMKKEIVLTAAMMLFSMVASTTFVSATEVYPKEYNTEGTITFEA
                     GDEGVTPPVDPENPDPNKPVDPSDPPSPGTGGALSIDYGSKFKFGTQKISTADKTYYA
                     AADVMNDGSRKPTYVQVTDRRSTLSGWKLSVSQPEQFKTASGDELVGAQLKFTKGQAV
                     SLVDPTYTPQTVNSELTLTPGGNNTLAINAKSGTGVGTWVYRFGANENENQDAVQLSV
                     PGKSVKLAQQYSTKLVWTLEDTPNN"
     gene            1199..3226
                     /locus_tag="IWM35_002655"
     CDS             1199..3226
                     /locus_tag="IWM35_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lectin"
                     /protein_id="HAP9920467.1"
                     /translation="MKKRRIVLILSSLLVTNIFPIIGYSLELNQLEHGNRRDEESKLT
                     STFDSLIESNQTQESSEDLSISSSGETTDNVEAEGSDKQGLEDAPISAVELPDDPNII
                     SIDKIFQTPIGTSTSILENGKLLQLNPAERSQRGAIWSNKKISLLSDFTFKSYLYLGN
                     QRDRAGDGMTFTLTGDPRMASNPSQVIGSPGMGIGSYSTRSGQPYIANALSIEFDTYK
                     NSGSSDRMDREITADNRGHGHVAFVTPKPNNNNYSGEHTGVTVAPTFLSNGTWRLLII
                     KWDAEQRSLTYDLEGVGHSSRIIPDLIRQFGSTEVNWGFTSSTGTFFQENAVAVTRLP
                     SNVTSTADVKVNDSDYGAIAEAGGNDKITIRNTLKVEGDFLEYKKMQTSIDLPQELTI
                     PEHILINGNQIDAKDILITGNRVTLNLTDYFIAGDTLIIELETTLQEVIPEKVLTMHF
                     EYLENGNLIEQSNEINLKIAKLKEIDIHVYYKDKDSQIDIAEMKTITGKIGEKYKETP
                     IDIEGYVFDSDSANTEGIFSENTEDIYFYYRKGKLYLSTAPQYVNFGKHKISSRPLSE
                     FGQFSGGLKIIDERAFGSWKLQLKQEKVLTNGDFELPEALSFVTSENTTVIGTSAVTI
                     FESDQKGESDLSSLLDSTAHRGIRIDIPVENQRIGTFEGKLSWILADVPGN"
     gene            3334..4431
                     /locus_tag="IWM35_002656"
     CDS             3334..4431
                     /locus_tag="IWM35_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HAP9920468.1"
                     /translation="MIKGAIFLRPKQQLFITLLVILGALFNNISVSASAFNFSVTPIT
                     SENQIDKRKTYFDLQLDPDQEVEVKAELRNDTEKEVKIDISVNSATTNSNVMVEYGKN
                     EIEKDMSLIFDLVDYVSYPQIVTLKPKSVQTVAFHVRMPNERFDGVLAGGITFQEIQT
                     EKDQTETKDQSLSIENEYTYIIALLMQQTLNEVAPNLTLHEVKPDQINARNVILANVQ
                     NDQKTYINQVVIETKITKKGHSEVLYQEEKEGLQIAPNTNFSFPTALNGQPLTPGEYH
                     LTMTVLGNENENGKFSRKKGNTTINYTNQWVFEKDFTIDGKVAKELNTTDVTIKKDNN
                     WIYILVASLFLLFILLCILLFVLRKKEEKEK"
BASE COUNT         1574 a          717 c          925 g         1261 t
ORIGIN      
        1 aaaaaatata atatacattt tttatttaat tataattaat attgattgaa gtctaggcag
       61 tcaagtcaca ttttgataga taggaggggg acttacgaat tattttaaag gctatgacag
      121 ctgtatttct agattgtgtt tgcatcaagt tgcaataaaa actagaaatc aaatcgggct
      181 gataatcatt tataactaat cgatacatag gaccaaatat aggtctatgt tcttttattt
      241 cataagtatc gtaaaacaga attttgctat ggattaatcc aatcgactga tatgtttagt
      301 aaataaagaa agaggaatgt caatgatgaa aaaagaaatc gtattaacag cagcaatgat
      361 gttattcagc atggtagcaa gtacgacttt tgtttctgca actgaagtat acccaaagga
      421 atataacaca gaaggaacga ttacatttga agcaggagat gagggtgtaa ctcctcctgt
      481 tgatccagag aatcctgatc ccaataaacc tgtcgatcca tcagatcctc caagccctgg
      541 aacaggaggg gcattatcga ttgattatgg atcaaaattc aaatttggga ctcaaaaaat
      601 ttctactgcg gataaaacgt attatgcagc tgctgatgtg atgaacgatg ggtctagaaa
      661 accgacatat gtccaagtga cagataggag aagtacctta tctgggtgga aattaagtgt
      721 ctctcaacca gaacagttta aaactgccag tggggatgaa ctagttggag cacagctgaa
      781 atttacgaaa gggcaggctg tttctttggt tgatccaaca tatacgccgc aaacggtgaa
      841 ttctgaatta acgttgacac cgggaggaaa caatacgtta gcaattaatg ctaaatcagg
      901 aacaggtgtg gggacttggg tctatcgatt tggtgcaaat gaaaatgaaa atcaagatgc
      961 ggtacagctt tcggtacccg gtaaatctgt caagttagcg caacaatatt ccactaaatt
     1021 agtttggaca ttagaagata cacctaacaa ctagtttact gaaaagaact gttaaaaata
     1081 aatacgtagt catattgtct tgcgaggtaa tcgagcaaaa attgtcaaag gctaggctaa
     1141 atttgtttgg atacgaatag ttcatctagg aatttaacga tcaggaggaa aagatatatt
     1201 gaaaaagagg aggatcgtgc tcattttaag cagtctctta gtgactaata tctttccgat
     1261 catagggtac tctttggaat tgaatcagtt ggagcatgga aatagaagag acgaagaaag
     1321 caaactcact tcgacattcg acagtttaat agaatccaac cagacacaag aaagcagtga
     1381 ggatttaagt atttcgagta gtggagagac cactgacaac gtagaagctg agggttcgga
     1441 caaacaagga ctagaagatg caccaatttc tgcggttgag ctgcctgacg atcccaatat
     1501 tatttctatc gataaaattt ttcaaacacc tatcggtact agcacgtcca tattggaaaa
     1561 tgggaagtta ctgcagttga acccagcaga aaggtctcaa agaggagcta tttggtcaaa
     1621 taagaagatt agtttactct cggattttac atttaaatct tatttatact taggaaatca
     1681 acgagataga gcgggagatg gcatgacctt tacattgact ggcgatccta gaatggctag
     1741 taatcccagt caagttattg gatcgccagg tatgggaatt ggctcatatt ctactcgttc
     1801 tggccaacct tacattgcga atgcgctctc gattgagttt gatacgtata aaaatagtgg
     1861 ttcttctgat cgaatggatc gagagataac tgctgataat agagggcatg gacatgtcgc
     1921 ttttgtcacg cctaaaccaa ataataataa ttattctgga gagcatactg gcgtgacggt
     1981 agctcctaca tttctttcta atggcacatg gcgcttgtta ataattaaat gggacgcgga
     2041 gcaaagaagt cttacttatg atttggaagg tgtcggtcac tcttctcgga ttatacctga
     2101 tctcattagg cagtttggtt ctacagaagt aaattggggg tttacttctt caacaggtac
     2161 tttttttcaa gaaaatgctg tcgctgtcac gcgacttcca tcgaatgtca catcaacagc
     2221 tgatgtaaaa gtcaacgata gtgattatgg ggccatcgct gaagctggag gaaacgataa
     2281 gataactatc agaaatacat tgaaggtcga gggggatttt cttgaatata agaaaatgca
     2341 gactagtatc gacctcccac aagaattaac tattcccgag catatcttga tcaatggaaa
     2401 tcaaattgat gccaaagata tcttgataac cggaaatcga gtcacgctaa atctgacaga
     2461 ttattttatt gctggagata cgttaataat cgaattggag acaactttgc aggaagtaat
     2521 tcctgaaaaa gtactcacta tgcattttga ataccttgaa aacgggaatt tgattgagca
     2581 atcgaatgaa ataaacctaa agatcgcaaa gctgaaggag atagatatcc atgtttatta
     2641 taaagataaa gacagtcaga ttgatattgc tgaaatgaaa acaatcactg ggaaaatagg
     2701 agagaagtat aaagaaactc ctattgatat tgaagggtat gtttttgact cggattcagc
     2761 gaatacagaa ggcatcttct ccgaaaatac agaggatatc tatttttatt acagaaaagg
     2821 caaactttat ctctcaactg ctcctcaata tgtcaatttt ggaaaacata aaatatccag
     2881 tagaccatta agtgaattcg gacagttttc aggtgggttg aaaattatag atgaacgggc
     2941 ttttggaagt tggaaattac aattgaaaca agagaaagtt cttacaaatg gagattttga
     3001 gcttccagaa gctctttcct ttgttacctc tgaaaataca acggttatag ggacatccgc
     3061 tgttacgatt ttcgaaagtg atcaaaaggg tgaaagcgat ttgtcctctc ttttagattc
     3121 tacagctcat cgtggaatca gaatagatat tcctgtagaa aaccaaagaa tcggaacctt
     3181 tgaggggaaa ctatcttgga tacttgcaga tgtcccagga aattaacagg aagagaagaa
     3241 tataacaaaa ttattgagtt tcaagaaggg cacaggtacc ataggctctt gtgtcccaca
     3301 gagacaagat tttgttatag tcttctctta catatgatta aaggagcgat tttcttgaga
     3361 ccaaaacaac aattatttat aacactccta gttattttag gggcactttt taacaatata
     3421 tcagtgtcag ctagtgcatt taatttttca gtgacgccga taacttcaga gaatcaaatc
     3481 gacaagagaa aaacttactt cgatcttcaa cttgatccgg atcaggaagt agaagtaaaa
     3541 gcggaattga gaaatgatac agaaaaagaa gtgaaaatcg atatttctgt gaatagcgca
     3601 accaccaatt caaatgtgat ggttgaatac ggaaaaaacg agattgaaaa agacatgagc
     3661 ctgatctttg atctggtaga ttatgtgagc tatccacaga tagtgacgct aaaacctaaa
     3721 agtgtccaaa ccgttgcttt tcatgtcagg atgccgaatg agcgatttga tggtgtattg
     3781 gctggtggga tcacattcca agaaatccag acggaaaagg accaaacaga aactaaggat
     3841 caaagcttat cgattgagaa tgaatacacc tatattattg ctttgttgat gcaacagaca
     3901 ctgaacgaag ttgctccgaa ccttactctt catgaagtta aaccagatca aatcaatgca
     3961 agaaatgtca tcttagcaaa cgttcaaaat gatcaaaaaa cctatatcaa tcaagtagtt
     4021 attgaaacaa aaattacaaa aaaaggacat tctgaagttt tatatcaaga ggaaaaagaa
     4081 ggattgcaga ttgcgccgaa tacaaatttt tcttttccaa ctgctttaaa tggtcaaccg
     4141 ctaaccccag gtgaatatca ccttacaatg actgttttag gaaatgaaaa tgaaaatgga
     4201 aaattttcga gaaaaaaagg aaatactacg atcaactata ccaatcaatg ggtatttgaa
     4261 aaagatttta caatagacgg aaaagtagcc aaggaattga acacgacgga tgttacgatt
     4321 aaaaaagaca ataattggat ttatatctta gtagcttcac ttttcttatt gtttatttta
     4381 ttatgcatac ttttgtttgt tttgagaaaa aaagaagaga aagaaaagta aggttaagat
     4441 aacaaatagt aaatttttat tattgtagga tggttgg
//
LOCUS       DACDXO010000114         4461 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E23605_2017
            SAMN12639989-rid9881463.denovo.120, whole genome shotgun sequence.
ACCESSION   DACDXO010000114 DACDXO010000000
VERSION     DACDXO010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639989
            Sequence Read Archive: SRR10023389
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741914.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:42:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,972
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 33 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 17 of 73
            Pseudo Genes (multiple problems)  :: 10 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4461
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639989-rid9881463.denovo.120"
                     /isolate="E23605_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            574..1008
                     /locus_tag="IWM35_002657"
     CDS             574..1008
                     /locus_tag="IWM35_002657"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9920469.1"
                     /translation="MKNKIIKIITCLFLFGMIVGASTPLNLFPFSARGIKVEAASSRY
                     NHNHRGFTCYKMNYYVTNKMCRDLKKNYNKLKTPAQIASFIPIGGVGTWVITNAFSSA
                     VDAMNVFVRAANQGKGVQLTYNAHFSNTTSYQYNDYARYVIK"
     gene            2298..3221
                     /locus_tag="IWM35_002658"
     CDS             2298..3221
                     /locus_tag="IWM35_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAP9920470.1"
                     /translation="MANEIVKYENRLNHIPLRKFNSREMNLFFSIASRVREKGVDEII
                     FSFHDLKKLSGYTERGEQFIKDLSSTYDKLLSINAWNDDGNTLTKFVAFTKYSIIRDK
                     QLVIISVNPEFKGLFNQLKNWTRFNLEQFTNLDSTYSKTMFRLLKQYRTQGWTEFSKE
                     KFFDLLDIPKSYWNSPSNIDKFILRPIKEELTPLFKGLTIRKKYGKGRGKPVIGYRFT
                     WKAEINHADDFSKGKQEDLRIKLFNIDHNGELTREEKWRAKDRILNLPLGTHEADFKQ
                     QQQTEEKQAEKRELSNKLKQDLLGNLQNLFD"
     gene            3237..3776
                     /locus_tag="IWM35_002659"
     CDS             3237..3776
                     /locus_tag="IWM35_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HAP9920471.1"
                     /translation="MADKTIKELAEELGVSKTAINKKVSDKNRKLWFAKNGNKFVINE
                     TGQKAIKSMFLVDNANLNRKLVGEKSKTKNHKNTNQVSELKLLSFLEKQLNRKDIQLQ
                     EKDNQIKQMQKLLDQQQQLTLQANQQIENLQEQLHLTYTEETPTTESTAFSEKKADNT
                     QPLIEKKWWQIWKSNRTNL"
BASE COUNT         1663 a          665 c          760 g         1373 t
ORIGIN      
        1 aaaaaaaact gtgttggtac tgggttctat ttctctagga atattaggtt ttagccccat
       61 gctattatcc gtaattggaa aaaacaattc caacagagaa cttaaattga attcaaatta
      121 ttacaacagt cctaacgaag aattgtttgt aaaagaaacg gataaataat aaaaaagact
      181 gctcttccga gcagccagtt aaaaaataat gagtgtttat tcttgaaaaa gcaacgcgct
      241 tataaagcgc taccaaaaat ttatcatatt ttatgaaaaa taacaatcca gtaaaaaatg
      301 actggtaaaa attcacaaag aaacggaaag aagcaactgc agaaaatcaa gccttaaaaa
      361 gagcttttta cctagaccaa ttaaaaatca caaagtgagc gtctctatcc attggtagag
      421 gggctacttg gcatagtgta cattatgtct actaaccttt aaaataacac gttttataaa
      481 ataaaccctc ttgacaatat ttaattttct gattattcta aatatataca gaaagcgctt
      541 tcctaggatt ttatatctaa ggaggaagta acaatgaaaa ataaaattat taaaattatt
      601 acttgtctat ttttgttcgg tatgattgtt ggagcaagta ctccgttaaa tctgtttcct
      661 tttagtgcta gaggtataaa agtggaagct gcaagttcac gatataatca taatcatcgt
      721 ggatttactt gctataaaat gaactattat gttactaata aaatgtgtag agatttgaaa
      781 aaaaattata ataagctaaa gactcctgct caaattgcat cttttattcc tattggtgga
      841 gttggaacat gggttattac aaatgctttt agtagtgctg ttgatgcaat gaatgtcttt
      901 gtaagagctg caaatcaagg aaaaggtgta caattaacat ataatgctca ctttagcaat
      961 acaacatcat atcaatacaa tgattacgct cgttatgtaa taaaataatc agaaagtgaa
     1021 actatctcat gaataaaaag attttagctg taattatttc aataatagtt ttaatcacta
     1081 tgttcttcat atttaggcta attttcaata taagcttaca aaaaagtatt ctttatctaa
     1141 ttcctatagc atttatctta gcaattttta gaagtattta cggaaataaa aactctaaat
     1201 agctaaaaat aaatcatact aaattttaca aaaagtggct agcattttct agccactttt
     1261 tgtattgtgg agcgtgggtt ttgaggggga ttcaaggggg agcttgcgcc cctttgacaa
     1321 gcggttattt gcaccaaata cggaggtatt tggctagcaa atatagagct tgccaaaccc
     1381 ttttagtgag ttttgtagaa agtaccttta aaggtacttt cacattacga acgatacgtc
     1441 cgtaggacgt actgaaaggg tgggacttat gagcaaatta atgcaaaatt tggctagcaa
     1501 aaaagctaaa aaatatatgc taataatcga gcgaaataaa cactttttca agtgagcgaa
     1561 tccgccatga caaagctgaa gcagtttatt tcagctttgt catggcgtgt gccgactttt
     1621 ttaaaagtct aggcacacga gcaaatatct ttatcaagct ctaatttgcg ttttaagacg
     1681 tttgaaacgc ctaagtatat aaatatataa gaataggttt aagagtgctt aaatcgaatt
     1741 ttagagggca tttagacaat tttagggttg ttagggcttg ccctgaccgc ctgtaaggcg
     1801 ttttaagaac aagataaggg atttatacta ggactgtctt aaaatgtctt aaacgaacgc
     1861 tcagagcttc tcaaagggtt ccttatgaac ggcttttagc aaaggagccg gaggcgactg
     1921 cgcagtataa tgtgagagca gagcgatcac acctttttag cgaaccgtag ggagcaaggt
     1981 ttggaattgg cagttttgat aaattcgcga acctgtttag agaaaaaagg agtgattttt
     2041 taggcgggaa aattagcctc ccctcttttt atatctcttt ttatatctct ttttatatct
     2101 cttttatgtc ggctgtgtcc ttactctccc aagcaattca gagttttttc aagtaggttt
     2161 tctacgcttt tcaagtaggt tttctacgct tttcaagtag gttttctacg cttttcaagt
     2221 aggttttccg acattattga gtatttttat aaagtatgtt acttttattt catactttat
     2281 aaaagtagga gaagcttatg gcaaatgaaa ttgtaaaata tgaaaataga ttaaatcata
     2341 ttcctctgag aaaatttaat agtcgtgaaa tgaacttatt tttttcaatt gcttcacgtg
     2401 tacgtgaaaa aggtgtagat gagattattt tttcatttca cgatttgaaa aaactaagcg
     2461 gttatacaga acgtggggaa caatttataa aagatcttag tagcacgtat gacaaattat
     2521 taagtattaa tgcttggaat gatgatggaa atactttaac taaatttgtt gctttcacaa
     2581 aatatagcat tatacgagat aaacaacttg ttataatttc agttaatcca gaatttaaag
     2641 gactttttaa tcaattaaag aactggacaa gatttaatct agagcaattt actaatttgg
     2701 atagcacata ttcaaaaact atgtttcgat tattaaaaca atatcgaact caaggctgga
     2761 ctgaattttc caaagaaaaa ttttttgatt tactagatat tcctaaaagt tattggaata
     2821 gtccgtcaaa tattgacaaa tttatcttaa ggccaattaa agaagaactc actcccttat
     2881 ttaaaggatt aactattcgt aaaaaatatg gtaaaggtcg aggtaaacct gttatcggtt
     2941 atcgttttac ttggaaagct gaaattaacc atgctgacga tttttccaaa gggaaacaag
     3001 aagatttacg tataaaatta tttaatattg atcacaatgg agaattaaca cgagaagaaa
     3061 aatggcgcgc taaagatcga atactaaatc ttcccttagg cactcatgaa gctgatttta
     3121 aacaacaaca acaaactgaa gaaaaacagg cagaaaaacg agaactttca aacaaactca
     3181 aacaagattt actaggaaat ttacaaaact tattcgatta agcttaagga gtatttatgg
     3241 cagataaaac aatcaaagaa cttgcggaag aattaggagt tagtaaaaca gcaattaaca
     3301 aaaaagtttc tgacaaaaat cgaaaacttt ggtttgcaaa aaacggaaac aagtttgtga
     3361 ttaacgaaac cggtcaaaaa gctataaaat caatgttttt agtcgataac gcaaacttaa
     3421 atcgaaaact agttggcgaa aaatcaaaaa ccaaaaacca taaaaacacc aaccaagttt
     3481 ccgaattaaa actcctttct ttcctagaaa aacagttaaa tcgaaaagat atacagctac
     3541 aagaaaaaga taatcaaata aaacaaatgc aaaaattact cgaccagcaa caacaactca
     3601 ctctacaagc gaaccaacaa attgagaacc tacaagaaca gctccattta acttatacag
     3661 aagaaactcc aacaaccgaa tcaactgcct tttctgaaaa aaaagcagac aatacacaac
     3721 cacttattga aaaaaagtgg tggcaaattt ggaaatctaa tagaaccaat ctctaaaatt
     3781 ttctagaagt tagttcggat agtagaggaa aaagttgtac tacctgatgg ggagatcact
     3841 caaattttta tgagaaagaa aaaatagttt acaactgtaa acaatcaatg tttatagttg
     3901 taaactatca aactatatgg tgacttattg cttaaaatag gttgaaatct cactcaattt
     3961 ttggtggaaa aacaaaataa attctaatcg attttgttga aaaagtcata aaaaaacaaa
     4021 tgtgatttta acaaaatttc aaaaaaatcg tttgaaattt cgtttatttt ttttgagaat
     4081 tttgatcatc gtagggtcat tgtagaatca ttatgagatc atcgtgggat catcgaataa
     4141 agtatgggaa aggcttgcgg tgcttggtat taagggcatt ctccgcctcc caggttcttg
     4201 accattttct cttataagtg ggatttttac cacgttgaga gaaaatggtc aagaacctgg
     4261 gaggcggaga aaacatacca agcaccacaa gcaaaaaaag ttagagtaac ttccaaaaaa
     4321 gaaaaatttt ttagaacaac ttttgaaaat ttaattttga tttatatccg ttcaatttaa
     4381 acatttgata aactgaaggc tagtatttaa aatgtgtata caagtggtag cgttaaaaaa
     4441 agtggaggta taacctatgt t
//
LOCUS       DACDXO010000115         4433 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E23605_2017
            SAMN12639989-rid9881463.denovo.121, whole genome shotgun sequence.
ACCESSION   DACDXO010000115 DACDXO010000000
VERSION     DACDXO010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639989
            Sequence Read Archive: SRR10023389
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741914.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:42:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,972
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 33 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 17 of 73
            Pseudo Genes (multiple problems)  :: 10 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4433
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639989-rid9881463.denovo.121"
                     /isolate="E23605_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(543..2048)
                     /locus_tag="IWM35_002660"
     CDS             complement(543..2048)
                     /locus_tag="IWM35_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP9920472.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDSFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDK
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
     gene            complement(2045..2731)
                     /locus_tag="IWM35_002661"
     CDS             complement(2045..2731)
                     /locus_tag="IWM35_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9920473.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            complement(2924..4345)
                     /gene="gndA"
                     /locus_tag="IWM35_002662"
     CDS             complement(2924..4345)
                     /gene="gndA"
                     /locus_tag="IWM35_002662"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HAP9920474.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDGAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
BASE COUNT         1239 a          919 c          729 g         1546 t
ORIGIN      
        1 tctttttgtt ttaacaactc tttaatctta tcagattcag aagagaatgt caacaacttc
       61 tttgtttatt ttttctaaac gtctttgcca ctcattagca gcgacttgat tatcatacca
      121 tctttattgt tatttgtcaa taactttttc gaagtttttt tcgataaaat caagctttat
      181 tagctatctc atccaaacga cttcgttatt ataacatgac atcaaaagtg cgtcaacact
      241 attttttata atttttagtg acaccttaaa ggaatcattt agaacaaata ataatatagc
      301 aatctcttta taaaattgca atagaaaatt ccctattttt atttattatt tttaaacacg
      361 aatattacag cattttattg ttttttcgtt catctaaaag atacttacat ttaattaacg
      421 gatgttattc tatttaattg ttcgctagtt tttttataat gacatccctg aatttctcac
      481 ttgcttgtat tttgtacctt attctttgtt ggatatataa aaaaacaagt aaagcacatg
      541 ctttacttgt cttctgcctt tgcgatagga ataaagattc taaaaattgt cccttgccct
      601 actacacttt ctgctaagat acgtccttta tagttttcaa ccagttgttt agcgatagac
      661 aaccccagac catttccacc cttcgttcta gctcttgctt tatccactcg ataaaaacga
      721 ttgaagatct tattaaggtc ttctttgggt attccttcgc caaagtcctg aatcgcgatc
      781 tcaaattcat tcagagtgga agaaatagag atatggactt cttttcgtgt agttgaatac
      841 tttactgcgt tatccaatat gataatgatt aattgttcaa agtgattacg ataaatttgc
      901 agggtcactt cttttgtcaa atcatcatct aaagtgatca catagtctgg atataatatc
      961 ttgaaattat tataaacttg ataggtgact tcttttcctt tagttgtttc attcgcataa
     1021 tgaacatcta cttgttctgc tcttgataag tctagcatct cttgaactaa gcttttcata
     1081 cggctgattt cttgcatgct ggcgcttaat gattcttcta atatctcagg atcatctttc
     1141 ccccaacgat ttagcagact aagatgccct tcaataattg caacaggtgt ccttaactca
     1201 tgagatacgt cttccacgaa ttgctcttgt tgttcaatat acaaacgcat acggtcaagc
     1261 atctcattga atatttcagc taaatctgcc aattcatcat ttgtgtcgat ttcaggcatg
     1321 tggatatctg attgcggatc tttacgtata gtatccattg tatctctgag taccttgagt
     1381 ggttttaaga aataagaaga taaaaagaaa cctaaaatac tacttaagat caaagaaaca
     1441 acttctaaaa caataagagt caataatagc ttatttcgaa tatcatagaa agaagataat
     1501 tcatagaagc tttgcgcata gccgatcttt tctcttgtac ctttagaata tataggctgg
     1561 atagataaaa agccagtttt tccttctagt gtaacgatcg ttggaacttt acgtgtagtc
     1621 tgaatcaggt ttcgactgtt ttcatgggtt ttaaaaatca agttctcgtc caaatcataa
     1681 acataaagat ccattgatgg ctgccctagc tcagctataa aggaatcgat cttcattaag
     1741 tttccttcga ccagtgaagt atgatctacg acctcatctc cgtcatttgt cgctttcgtc
     1801 agattacgat acgtatttac taatgtcagt tcttcatccg agttagccaa acgagaagct
     1861 acttctgaaa tcgtacgctc tacattgttt ctttcttttg ccacaatcag attgatagat
     1921 gatttatagg taatgacagc gaaaattgta aacacaacga atatgaagaa agaactcgca
     1981 aaggcccact tgattgtcaa ggagggccct ttcagttctc ttttttccga ttttttcggc
     2041 tttatcacga gcgcatcaca taacctgttc cgcgtacagt ttggatatag ctttcctccc
     2101 cgggaacgtc aattttgttg cgaagataac gaatatatac gtccactacg tttgtttcaa
     2161 cttctgtttc gtatccccac actttattta gtaaaacgtc acgtgctaat accacgttca
     2221 cgttttccat taaagtaagc agcaactcgt attctctttt tgttagttca attacttcat
     2281 ttccgcgacg tacgacacgg ttttcttttt cgatagtcaa atcgcgataa gtaatagtag
     2341 tttgtttagc cacattttta tcgccttcaa tatcgattcg acgcaacaat gcacgaagac
     2401 gtgctaataa ttcttcgata gcaaatggtt tgacgatgta gtcatctgct ccgtggtcaa
     2461 gtccagatac acgatcgatc acagaatctc ttgcagtcat cataatgatt ggtgtatttt
     2521 tgacttggcg tacacgtctg cagacttcta aaccgttcaa ttctgggagc atcagatcca
     2581 acaatatggc atcccactca ttatttaatg ccgcatctaa accagttcgg ccgttgtagt
     2641 ggacttctgc gttataccct tcatgcttca actctagctc gacaaatcgt gccaagttct
     2701 tttcatcttc aatgattaga atattgctca tttattattt tatcctttct attctactgg
     2761 ctttttaaaa actctatcat tataccgata atatgcttaa aaatctatag tcattttatc
     2821 atggatagca attttaaaaa agagctgaag tctactcata acaatcgact tcagctcttt
     2881 cgtcctcttt ttattttctc atttcctaac agccgaaaga ctattattct tcatggtacc
     2941 atgagtaatg gaagattcct tctttgtcga cacgctcata ggtatgtgcg ccaaagtaat
     3001 cgcgttgggc ttggattata tttgcaggca gacgttctgc tctgtatgaa tcgaagtaag
     3061 cgatggctga tgagaaagtc ggtacaggta cgccagcttg gacagccaag gcaacgacgt
     3121 ctctgactga ttgctgatat tttttcgtga tttcaacgaa gtaatcatct agcaataaat
     3181 tttcgatctc aggatttttg tcataggcat ccgtaatttt ttgcaagaat cgagctcgga
     3241 tgatacatcc agcgcgccag atttttgcga tttcaccaaa tggcaagtcc caatcaaatt
     3301 cttttgaagc gacacgtaat tgagcaaatc cttgggcata actcatgatt ttactgaaat
     3361 acaacgcttc tcggattttt tcgaccaatt cttttttgtc tcccgtgaac tcaaaatcgt
     3421 tcgtacgaga caaaatcttg cttgcttgta cgcgttcttc tttataagca gaaatatagc
     3481 gggcaaatac agattcagtg atcaatggaa gtggcacacc taagtcaagc gcgctttggc
     3541 ttgtccattt acctgttcct ttattcccag ctgcatcaag gatcacatct actactggtt
     3601 tacctgttcc ttcatcgtct ttacgtgtca gaatgtcagc agtgatttct atcaaatagc
     3661 tgtctaattc gccttgattc cattctttaa agatatcagc catttcttct actgaaaggt
     3721 ttaaaatatt cttcattaag tcataagatt cagcaatcag ttgcatatct ccatactcaa
     3781 taccgttgtg taccattttc acataatgtc cggctccatt tgggccaata tatgtgacac
     3841 atggtgctcc gtcttctgct tttgcagaaa tttgctctaa gattggtgca actagcgcat
     3901 aggcttcttt ttgtccacca ggcatgattg aaggtccttt taaagctcct tcttcaccac
     3961 cggaaacgcc tgtaccgata aagttgatgc cagagttggc taactcttca ttacgacgca
     4021 tcgtatcttt aaagaatgta tttcctccgt caatcaaaat atcgccttta tcaaggtgag
     4081 gaagtaactc ttggattgtt gcatctgttg ccggtcctgc tttgaccatt agcaagatcc
     4141 gacgtggttt ctcgatagat tctacaaatt cttcgattgt atatgttgct ttgaaatttt
     4201 tatctgggtg ttcttctact acagcggttg ttttagcccc tgtacgattg aataaagcca
     4261 ctgaatagcc gcggctttct atgttcaagg ctagattttt acccataacg gccataccga
     4321 caacgccgat ttgttgtttt gacatatgaa ctcctcctac atacgggatt tctccctaat
     4381 ctcttacatt atagcttaac acggctacat tgcaaactaa agaacaacat att
//
LOCUS       DACDXO010000116         4425 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E23605_2017
            SAMN12639989-rid9881463.denovo.122, whole genome shotgun sequence.
ACCESSION   DACDXO010000116 DACDXO010000000
VERSION     DACDXO010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639989
            Sequence Read Archive: SRR10023389
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741914.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:42:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,972
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 33 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 17 of 73
            Pseudo Genes (multiple problems)  :: 10 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4425
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639989-rid9881463.denovo.122"
                     /isolate="E23605_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            690..1658
                     /locus_tag="IWM35_002663"
     CDS             690..1658
                     /locus_tag="IWM35_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP9920475.1"
                     /translation="MDVAKTVNAIVEKNDGEISGIYLVACGGSLVDMYVSDYFLKAEA
                     SKCITGLYTANEFNHVTPKQLDDKSVVILCSHGGNTAETVKAGEVAQARGAVTVGLTH
                     NKTADLLKVSDHSFLYEWGDDAKIVNNPMAIILDLTVSLVNVTETFEHYGKFREGMKV
                     IDTVIENGIKQVQDRIKVFADKYKDETMFYILGSGPSYGHAYGFSICSLMEMQWLDST
                     SVHSGEFFHGPFEVTDKDTNFILLMSIGRTRALDERAKRFLDKYAERVEIVDAKELGL
                     DMISDEVSEFFNPILFYSILSEYRSALADVRNHDLDVRRYMGKVEY"
     gene            1669..2484
                     /locus_tag="IWM35_002664"
     CDS             1669..2484
                     /locus_tag="IWM35_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HAP9920476.1"
                     /translation="MKVLGLGDNVVDKYENLGIMYPGGNSLNFAVFAKKLGHESSFMG
                     VFGSDEEAAHVLSAIKEIGLDNSHSRFEKGENGCARVKIDEGDRIFLGSNSGGVTREY
                     PIQLTEEDREYLNQFELIHMGLYSHVNHLLEELQNVSGKISYDFSDDFTEEEVQRAID
                     KVDFGFFSIEDFSDEEVKQFLMKHFCERNDVLIATRGEKTAIGYDGAKFYEVNPVLEE
                     PVDTMAAGDSFLTAFLLYFLEGDDIVTAMKKGNEFAGKSCLVDGSFGFGIHYD"
     gene            2552..3262
                     /locus_tag="IWM35_002665"
     CDS             2552..3262
                     /locus_tag="IWM35_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP9920477.1"
                     /translation="MLDTNAQLPLYLQLKEILKNKINTGEYPENTKIPAESELIESYE
                     VSRITVRKAVEELVEEGYLLKKQGKGTFVNRHKVFRKIEYIMGFSESCKSNGFTPTSI
                     VLERKIIPATEGMAKKLDIAQGDDVLYIQRKRMADNIPIMLENNYFSYERYSFLMEEE
                     LTDSLYEVLKVQGTLAINPGETALEIVTADDQIAKIMEVAVGTPFFFMDTLINDQNNI
                     PIHVGNQYYLGEVYRFYL"
     gene            3298..>4425
                     /locus_tag="IWM35_002666"
     CDS             3298..>4425
                     /locus_tag="IWM35_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HAP9920478.1"
                     /translation="MKQILRSKMIFDGEQPPFKGYIVINEGIIEKVENNWNYSDLLNE
                     NTELIAYDDQFVMPAIHDNHVFFSGYLAMNAGIDLSDTSSANEAVERIKAVMNHKKAD
                     EPIYAHGWDAALWKTDPSEELLNEINHFGPITAIDKNRSHCWMNAIAKQTYGFTEKET
                     SAESRVLLIKEMLANKKLLAEVYAEFEALLLSKGVISMKEIVFDDAEFLSNIPQRKIL
                     SNLYVQAVQEPLQHELLYEYKEKVFPEKVRFGGVKIMVDGVVADRSGDIYGVYQSGVA
                     SPEIDYEAIKAEVAAFNEVEIPCCLTTEGDKAGYKAASILVEFGKRLPEGVFNSISDL
                     EMVTQKTAEKMNEGQVVAEVYPQILGLNQTLEEAYMSTVIKN"
BASE COUNT         1490 a          661 c          921 g         1353 t
ORIGIN      
        1 atacaaataa ttcaccaaaa aggtgagtaa tgccattttt gaaaattgag cttgttttat
       61 gtctacatct gtatccgtat ataatccaaa ggattataaa ctcagaagag tctttatttt
      121 ttctgaatat aactactgta tcaaaaacaa tcgattatga gacaaaacaa aatgacctct
      181 tttatttaag aaaggaggtc attttttgta tcataaattg tattaaaatc aatgtatcac
      241 tttttaatca gttttaagac cataaattgc gtactaaaag aaattctgca attcaaaata
      301 aagcatttta agccattttc aaatttaagt gcacccatct acccgtcaaa tcactttatt
      361 ttccactcca gtagtttcct aattcaactg tgtgcaaaaa cgatttcttc taaacaaatc
      421 aagacaaact tataaacctt catcccagca ccataattat ttaaatcata ggcatttttc
      481 gaaattagta tatgtactca agtttcactg cagattattt atatttttta gagaaataaa
      541 tttttcctat tgacaaacac aaagaaagcg gatacaatgc aattatgtca catgacgtta
      601 tatgacgtta tgtgatatct ttacatttaa tcagttactt ttattttgga taaaattaac
      661 atttttaaaa ttttaaggag gaaatgttga tggatgtagc aaaaacggtt aacgcaattg
      721 tagaaaaaaa tgatggagag atttctggaa tttatttggt ggcttgtggc ggttcgctgg
      781 tagatatgta tgtctcagat tattttctta aagccgaagc atcgaagtgt attacaggct
      841 tatatacagc gaacgaattt aaccatgtaa ccccaaagca attagatgat aaatctgttg
      901 tcattctatg ttctcatgga ggaaatacag cagaaacggt aaaagcagga gaagtcgctc
      961 aagctagagg agctgtgact gttggtctga ctcataataa aactgcggat ttattaaagg
     1021 tttctgatca tagtttcctt tacgaatggg gagatgatgc aaaaatagtc aataatccta
     1081 tggccatcat tcttgattta actgtatctt tggtcaatgt taccgaaaca tttgaacatt
     1141 acggtaaatt cagagaaggc atgaaggtta tcgataccgt aattgaaaat ggcattaagc
     1201 aggtgcagga tcgtattaaa gtgtttgctg ataagtacaa agatgaaaca atgttttata
     1261 tcttaggtag tggaccatca tatggacatg cttatggttt ttcaatttgc tctttgatgg
     1321 aaatgcaatg gcttgattca acttctgttc attctggaga attcttccat ggaccttttg
     1381 aagtaacaga caaagatacg aattttattc tcttgatgag cattggacgc actcgtgctt
     1441 tagatgaacg ggcaaaacga ttccttgata aatatgcaga aagagtagaa atcgttgatg
     1501 caaaggaact aggattggat atgatatcag atgaagtttc tgaattcttc aatcccattt
     1561 tattctacag tattctttca gaatatcgtt cagctttagc agatgtacgc aatcatgatt
     1621 tagatgttcg tcgctatatg gggaaagtgg agtactagga ggattaaaat gaaggtatta
     1681 ggcttaggtg ataacgtcgt cgataaatat gaaaatctgg gaattatgta tcctggagga
     1741 aattcattga attttgcagt ttttgcaaaa aaactgggac atgaaagtag ctttatggga
     1801 gtttttggct cagatgagga ggcagctcat gttttatcag caataaaaga aataggactg
     1861 gacaacagtc actctcgctt tgaaaaaggc gagaatggct gtgccagagt gaagattgat
     1921 gagggcgacc ggattttcct tggcagcaat tcgggaggtg ttactcgtga atacccgatt
     1981 cagcttactg aagaagacag agaatattta aaccagtttg aattgattca tatgggatta
     2041 tatagccatg tcaatcatct attagaagag ttacaaaatg tttctggaaa gatttcttat
     2101 gatttttctg acgactttac ggaagaagag gttcagcggg caattgataa agttgatttt
     2161 ggcttctttt ctatagaaga cttttccgat gaagaagtaa agcagttttt gatgaagcat
     2221 ttttgtgagc gaaatgatgt attgattgca acccgaggag aaaaaacggc tattggttat
     2281 gatggagcaa agttttatga agtgaacccc gtgctagaag aaccagttga tacaatggca
     2341 gcgggagatt ctttcttaac agctttccta ctttattttc tcgaagggga cgatatagtc
     2401 accgcgatga aaaaggggaa tgaatttgct gggaagtctt gtctggttga cggttcgttc
     2461 ggctttggta ttcattacga ttgagcttgt tttaaattcc tgcatttgat aacctaaaga
     2521 aagaaatccc ggtaaagtgg aggttaaaaa aatgctggat acaaatgcac aattaccatt
     2581 gtacttacaa ttaaaagaaa tattaaaaaa taaaatcaat acaggggagt atcccgagaa
     2641 tacgaaaatt cccgctgaat cagagctgat agaaagttat gaggtcagcc gaatcacagt
     2701 gagaaaagca gtagaagaac tggtagaaga agggtaccta cttaaaaaac aagggaaagg
     2761 aacttttgtc aaccggcata aagtttttcg taaaattgaa tatattatgg gtttcagtga
     2821 gagttgtaaa tctaatggtt ttacaccgac tagcattgtt ttggaacgca agatcattcc
     2881 agcaactgaa gggatggcaa aaaaactcga tatcgctcaa ggtgatgatg ttttatatat
     2941 ccagagaaag cgaatggcgg ataacatccc cattatgtta gaaaacaatt atttttctta
     3001 tgaaagatat agtttcttaa tggaggaaga gctgactgat tctttgtatg aagtacttaa
     3061 agtccaaggt accttagcca tcaatcctgg ggaaactgca cttgaaattg tcacagctga
     3121 tgaccaaatt gccaaaataa tggaagtagc tgttgggacg ccctttttct ttatggatac
     3181 tttaatcaac gatcaaaaca acataccaat tcatgtaggt aatcaatatt acctgggaga
     3241 agtatatcgt ttttacttat gatttcctgt ttggatttca aggagtagat tttatttatg
     3301 aagcaaatat tgcggtcaaa aatgattttt gatggagagc agccaccttt caaaggttat
     3361 attgtaatca atgaagggat cattgaaaag gtggaaaaca actggaatta cagtgatcta
     3421 ttaaatgaaa acacggaatt aattgcttat gatgatcaat tcgttatgcc agcaattcat
     3481 gataatcatg tatttttcag tggttatttg gctatgaatg ctggcataga tttaagcgat
     3541 acatcttcag ctaacgaggc tgtagagcgt attaaagctg taatgaacca taaaaaagca
     3601 gatgaaccaa tttatgctca tggctgggat gctgccctgt ggaaaactga cccatcagaa
     3661 gagctattga atgaaataaa ccactttggt ccaatcacag cgatcgacaa aaatcgcagc
     3721 cattgttgga tgaatgctat tgctaagcag acttatggtt ttacagaaaa agaaaccagt
     3781 gctgaaagtc gagtattatt aattaaggaa atgctagcaa acaaaaagct tttagcagag
     3841 gtttacgctg aatttgaagc attactgctt tcaaaaggtg ttatatcaat gaaagaaatc
     3901 gtttttgatg acgcagaatt tctttctaat atacctcaga ggaaaatact tagtaaccta
     3961 tatgtgcagg cagtccaaga acctctacag catgaacttc tatatgagta taaggaaaaa
     4021 gttttccctg aaaaggtccg ctttggtgga gtgaagatca tggtagatgg tgttgttgcg
     4081 gatagaagtg gagatattta cggtgtttat cagtcaggag tagcttcacc cgagattgac
     4141 tatgaggcaa ttaaagcaga agttgctgca ttcaatgaag tagagattcc ttgctgtttg
     4201 acaacggaag gagataaagc gggttataaa gctgcgtcaa ttcttgtcga atttggaaag
     4261 cggttacctg aaggcgtttt taattcgatt agtgatttgg aaatggttac tcaaaagaca
     4321 gcagaaaaaa tgaacgaagg acaagtagta gcagaggtat atccacaaat tctcggcttg
     4381 aatcaaacgt tagaagaggc ttacatgtca actgttataa aaaat
//
LOCUS       DACDXO010000117         4345 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E23605_2017
            SAMN12639989-rid9881463.denovo.123, whole genome shotgun sequence.
ACCESSION   DACDXO010000117 DACDXO010000000
VERSION     DACDXO010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639989
            Sequence Read Archive: SRR10023389
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741914.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:42:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,972
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 33 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 17 of 73
            Pseudo Genes (multiple problems)  :: 10 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4345
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639989-rid9881463.denovo.123"
                     /isolate="E23605_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(387..1853)
                     /locus_tag="IWM35_002667"
     CDS             complement(387..1853)
                     /locus_tag="IWM35_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP9920479.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKKITEPETVVQP"
     gene            complement(2144..4228)
                     /gene="cadA"
                     /locus_tag="IWM35_002668"
     CDS             complement(2144..4228)
                     /gene="cadA"
                     /locus_tag="IWM35_002668"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HAP9920480.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQETKKVVKELEPDVKVTEIDKEKVSEHGNEKERNTLFRILFSLAGIALL
                     LLFDFNEPIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTTEGLMTVNPEDVQIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     GAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDTKIDPKKFMQYIASAEQFSTHPIAQSVLEGYAGPLLP
                     TTNIQEFAGEGILAEVDGKQVLAGNHKLMERFEISFPLSQEIGTLLYLAIDQSYSGYL
                     VIADTLKEDAVDALVQLKQAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDKV
                     QRLEEILQRNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQPS
                     KIAEAIHLAKKTLKIVKQNIVFAIGIKILVLALGAFGFASMGDAVFADVGVTVLAVLN
                     AMRSLHVKKEDE"
BASE COUNT         1310 a          927 c          696 g         1412 t
ORIGIN      
        1 aataaatggt gttcaatcag ctgaccttca gcattagctc aaaaagcggc aaaatatgag
       61 gagctatttt tccgtttgtt tctctaatac tcaggtcaaa tcgctgattt tacaacctct
      121 tccttaaacg gtgttccaaa gcttgcatct gcggtaataa gcccagccaa acgaaaaata
      181 tgaggggcta tttttgtttg gctgttctta ttacttgatg caggacagct ttttcacaac
      241 ctctgattca cggtgttcca gaagtagctt cttcgaaaaa caagccgaaa cttcacaaaa
      301 atttgagaag caattttcgt aaattccttc ttatttcttg aagctgacca cttctgtcac
      361 aacctcttgt aacaagtatt tctattttaa ggctgaacca ctgtttctgg ttcagtgatt
      421 tttttgtttt tgtctcgacg agcaattgct ggcaagatca tacctaataa gatcaagacg
      481 attggtgtga tgatattcgt aaataattgg aaccaccatg ctttaggatc agcagtataa
      541 tctacttttg gcaccattcc taggatacat gcaaacgcag tgaaggcaaa gcaccaagca
      601 ccaaagataa aaccgatttt tggattttta acaaatttat attcagaatt gaatttctta
      661 taagctttat tcaacatcat atatgccaag aacacccata ggtaacgcat tggcatgaca
      721 actgagttca agttcgtcaa ccattttact aattcattca tatctttgat accaaaaatc
      781 ggcaacaaaa taatgattcc tactagtata cctgtcagaa gataaccatt caccaatgta
      841 cctttacgat tacgtttacg tagccagttt ggtacaaatt ctggatccgc atctgctaac
      901 aagattttta gtggtgcatc aattgaaaag gctaatgcgg agatttgtcc aatcatgttt
      961 gtcaatgcgt aaatgatcat caatgagtta ccgataccat agtaattacc taaaatttga
     1021 aaagcactgt atgccccatt tgccatcaag tctttaggaa tattatcact agcaaacagc
     1081 atacccattg cgactgagcc taatacagca caaatcccta ccattgctgc taagaagatc
     1141 atccctttag gaaattcctt tgcaggattt cttgtagagt tgacataagg ggatattttt
     1201 tccgcccctc caactgcaaa tactagcatc gaaacggttg tgaaataact gaaatcaaat
     1261 gttggaatat atgttttcac ttgattcata tgtggtgtgg caaattccat cgttggattg
     1321 atgaacggtg ccgttacagc taaaagaata aataagatcg acatgacgaa catcgctgtt
     1381 ccggccagtc cgccaatcac tttcaaagta gaaagtcctt ttgttgataa atataaaaag
     1441 gcaagaaata taataagtga aataactgct accatttgta ctgacatcgt atttacgata
     1501 tttccatttc cttggactgc ccaaccacca gcaatcagaa ttgcttgagg cttttgtgcc
     1561 aaataaggaa tatggacgac ccagtaagtc caagcggcat agtatgccag tcgtttatta
     1621 gatgtcgtat tttcgaccca tgaactgacg cctcctttac tatccttgaa ggctgatcct
     1681 aattgtccaa cgatcaatgc gtatggaata aaataaatgg ccaaaatcaa tagccaagag
     1741 gtgacaactg atactccttg ctgagcatag ttattaacta cattccccat gccccaaacc
     1801 atgttaaatg caattaacgc aacagtaaac cagcgtaatt gtttgttgtt cattcgttct
     1861 ctcctttatg ataaaaattt gttatccaga tgtaatcata ttcaattttt aacaaactta
     1921 caagacagtg ttattttatg tgtgtattct tttatttaat acacatcgtt ttttcatatt
     1981 ttttccatca aaaaaataag ttattttctt ttatttcatt gaccttattg aatttcaagc
     2041 tattttgaat tttctgcaaa attaaaaaca ttagttattt tataatccta tctattttct
     2101 ttacattttt cattaagaaa aggatataaa aaaaagagga cgttcattcg tcctcttttt
     2161 ttacatgcaa gcttcgcatg gcattcaaaa cagctaatac tgtcacccca acatctgcaa
     2221 atactgcatc tcccattgat gcaaaaccaa aagcacctaa ggcaagtacc aaaattttta
     2281 tcccaatagc aaagacaatg ttttgtttga cgattttcaa tgttttcttt gctaaatgga
     2341 ttgcttctgc aatcttcgat ggttgatcat tcatgatgac tacatctgct gcttcaattg
     2401 cagcatcgct tcccaatcca cccattgcga ttcctacatc tgctcttgct agcaccggtg
     2461 catcgttgat tccatctccg acaaatgcgg tctttttatt gtttctttgt aagatttcct
     2521 ctaaccgctg cactttatct tcaggaagca attcgctata tatcttgtcc acgcctactt
     2581 gcttcccgat atgatcagca atttttttcg agtcgcccgt caacataact gtgttcttca
     2641 caccagcctg ttttaattga accaatgcgt ctacggcatc ttctttcaag gtatctgcaa
     2701 taacaagata accactgtaa ctttgatcga tcgctagata aagcaatgta ccgatttctt
     2761 gacttaaagg aaaagagatt tcaaatcttt ccatcaattt gtgattacct gctaatactt
     2821 gtttgccatc aacttcagct aagatccctt cacctgcaaa ttcctggata ttagttgttg
     2881 gtaaaagcgg acctgcgtag ccttctaaaa cagattgagc aatcggatgt gtagaaaact
     2941 gttctgcact cgcaatatat tgcatgaatt tctttggatc gatttttgta tcgatcgtct
     3001 ggatcgaaaa gtcacctttc gttaatgtgc cggttttatc gaataccact gtctcggttt
     3061 gcgcaagcaa ctccaagtaa tttgaacctt ttactaatac accgatttta cttgcgcctc
     3121 cgataccgcc aaagaaacta agtggcacgg aaatcactaa tgcacatgga catgagatca
     3181 ctaaaaatgt caatgctcga tagatccatt cagaaaacgc cgctccagaa acaacaagtg
     3241 gaggtaaaac agctaataaa acggccaatc ctacaacgac tggtgtgtaa tagcgcgcga
     3301 atttagtaat gaaattttca gctggtgctt ttttactgct ggcattttct actaattcta
     3361 ataatttgct gatagtagaa ttttcataag atttttctac ttgaactaaa aatggtttat
     3421 ttttattgat aaatccgctt aatactgttt caccaacata tacagattta gggactgatt
     3481 ctcctgtaag tgctgacgta tcgactaatg attctccttc gatgatcgtt ccatctaacg
     3541 gtacacgttc accaggcttc acaagtacaa actgaccgat ctgcacgtct tcagggttca
     3601 cggtcatcaa tccttccgtc gtctgcacgt gagctgtttc tggacggatc gccatcaatt
     3661 cgcgaatcga ttttcttgaa tgactgactg ccaatccttg gaaatactcg ccgatttgat
     3721 aaaatagcat cactgctacg gcttctggat attcaccgat caacatagcg ccaatcgtag
     3781 caacagacat aagaaaattc tcatcaaaga tttttccttt gacgatattc atcactgcct
     3841 tcttcactac atcatatcct atcagcagat aaatcaataa ataccctatc aagcgaattg
     3901 gttcattaaa atcaaataat agcaataaag cgatacctgc aagggaaaat aaaatccgga
     3961 acagcgtatt tctctctttt tcatttccat gttcagatac tttttcctta tctatttctg
     4021 ttactttgac atctggttct aattccttca ctactttttt tgtttcttgt tcaatcgtag
     4081 ccaaatgatc ttcctctgcg tcaatcgtta gttttccaga agtgaagttt accgttgctt
     4141 ccttgactcc attgatcttc tgaactcctt tttcgatttt cattgcacag ttcgcacaat
     4201 ctaaaccttc taatctgtaa cttttcattt taatgacctc ctcgcctcaa catatgaaca
     4261 tctcttcata actaattata aatgaatgat tattcatatg tcaaggtatc cgaaagaaaa
     4321 aatgaaaaac atctgcatcg gaaaa
//
LOCUS       DACDXO010000118         4282 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E23605_2017
            SAMN12639989-rid9881463.denovo.124, whole genome shotgun sequence.
ACCESSION   DACDXO010000118 DACDXO010000000
VERSION     DACDXO010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639989
            Sequence Read Archive: SRR10023389
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741914.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:42:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,972
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 33 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 17 of 73
            Pseudo Genes (multiple problems)  :: 10 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4282
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639989-rid9881463.denovo.124"
                     /isolate="E23605_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(37..1257)
                     /locus_tag="IWM35_002669"
     CDS             complement(37..1257)
                     /locus_tag="IWM35_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAP9920481.1"
                     /translation="MKLIGIVGSAAELSYNRKLLTFIQKQYHELFNLEIIEIKDLPLF
                     NQDDDQTNSPAIQQLNRKILMADGVIIATPEHNHTVPAGLKSALEWLSFKIHPLENKP
                     VMVVGASYYDQGTSRAQLHLRQILDAPGVNALVMPGNEFLLGKAKEAFDQEDRLIDPR
                     TVDFLGSTLASFVKFIKVVSTLAGPKKLPPEDLHATGTIATTIPDVDMSDDHWLEKAS
                     EAVGAVSGNTYVKLNRGLLTVDQINYFLDSMPMELTFADNNNQFLYYNLRQEAKEMLA
                     ARVPGQVGNPLANCHPEKSYKNVEWVIQQLRSGATDAVRVHVPKQVPEKYIVHNYQAM
                     HDENGEYAGINEYILDFKPIIEWYLQQTGQQLVGNEDAVSSASVHGGKTDAVTSASTH
                     DETAVDAVSSASVK"
     gene            complement(1277..1882)
                     /locus_tag="IWM35_002670"
     CDS             complement(1277..1882)
                     /locus_tag="IWM35_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005234653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAP9920482.1"
                     /translation="MKFIGLVGTNSTRSTNRELLQFMKNYFVNEAEIEIVEIKELPLF
                     NKPENTELPNVVKEIAGKIEQADGVIISTPEYDHAVPASLINALNWLSYGIFPFVDKP
                     VMITGASYGTLGSSRAQAHLRQILDSPELKARIMPSSEFLLGHSLQAFDEQGRLIDEE
                     QVAKLRELFKDFLLFVTITSQLQHAHQANKREAESFSWETI"
     gene            complement(1931..2863)
                     /locus_tag="IWM35_002671"
     CDS             complement(1931..2863)
                     /locus_tag="IWM35_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HAP9920483.1"
                     /translation="MNQVIEETKIWQMMGTTITLQVGHEEPSRLLAELGEWLHVYEHR
                     FSAHDATSELMAINQAAGRQAVIVHPELFELIKLGKAHSCARNSYLNIAIGPVVQKWH
                     IGFEDALVPTKAEITELLLLTDPHQIQLDEKMKTVFLTKPGMMIDLGALAKGYIADKL
                     ITHCRERNAAFALVNLGGNIVVFGESPRRSDGAWRIGLRNPKGSREESLAVVKLNNQS
                     LVTSGIYERYLQNGSSVYHHIIDPKTGFPVKNDVASLTILSDSSVDGEIWTTRLFGAS
                     SLEVLKALEEQPGIEGAVVTKENQFLYTKGFEKI"
     gene            complement(3284..4144)
                     /locus_tag="IWM35_002672"
     CDS             complement(3284..4144)
                     /locus_tag="IWM35_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9920484.1"
                     /translation="MNIEKFITARKAANLSQKEVSEGICTQATLSKFENNGQVPSLKI
                     LIKLCNRINLPLVELFSSMNVINTQLDEQLEKAEFSLITSEYEQLTQILAAIDPKVID
                     ESELIARYYYLKGFEMIFTNAPITDVLFHFDQLLMAETANTLFRLLAYTGIGMVYARE
                     KELEKAEVYFNRVFEKIYTYPIRTTEDIWRVLNIVFHCGVFFAQDGDLETSDALLNYA
                     IDICSDNHLTYYLARAAAQLAKNAQAKNKSKAEVLELIQDALAFAKINKNKKLIDELN
                     HMKKMILLIN"
BASE COUNT         1219 a          861 c          701 g         1501 t
ORIGIN      
        1 tttattcttt atttaaaact ttgcaacaga acctttttat tttacagaag cactcgaaac
       61 tgcatctact gcagtttcat cgtgtgtgct ggcgctggtt actgcatctg tttttccacc
      121 atgaacagat gcacttgaaa ccgcatcttc atttccaaca agttgttgac ccgtttgttg
      181 taaataccac tcaatgattg gtttgaaatc aagaatgtat tcattgattc cagcatactc
      241 tccattttca tcatgcattg cttggtaatt atgcacaata tatttttctg gcacttgttt
      301 tggtacatga acacgtaccg catctgttgc tccgcttcgc agttgttgaa ttacccactc
      361 tacattttta tacgattttt ctgggtgaca gttggctaat ggattcccta cctgaccggg
      421 cacacgagca gctaacatct ctttcgcttc ttggcgcaaa ttataatata aaaattggtt
      481 attattatct gcaaaagtta attccatcgg catcgaatct aaaaagtaat tgatttgatc
      541 cactgtcaat aacccacgat ttaatttgac gtaagtattt ccagaaacgg caccgactgc
      601 ttcagaagct ttttccaacc aatgatcatc tgacatatca acatcgggta ttgtcgtagc
      661 aattgttcct gtcgcatgca aatcttctgg tggaagcttt ttaggccctg ctaaggtaga
      721 cacgacttta atgaatttta caaaacttgc taatgtactg cctaaaaagt caacagttcg
      781 tggatcaatc aagcgatctt cttggtcaaa tgcttctttt gcttttccca ataaaaattc
      841 atttccgggc atcactaatg cattcacgcc tggtgcgtcc aaaatttggc gcaagtgtaa
      901 ttgcgcgcgt gaagttcctt gatcgtagta agaagcaccc acaaccatta caggtttatt
      961 ttcaagtgga tggattttaa atgatagcca ttcaagtgca cttttcaatc ctgctggaac
     1021 cgtatgattg tgttctggcg tagcaataat taccccatca gccattaaga ttttgcgatt
     1081 tagctgttga atagcaggac tatttgtttg atcatcatct tgattgaaca gaggtaaatc
     1141 tttgatttca atgatttcta aattaaatag ttcatgatat tgtttttgga taaatgttaa
     1201 taatttacgg ttatatgaaa gttcagctgc tgatccgacg atacctatta atttcatctt
     1261 tgtttgctcc cctcgattaa atcgtttccc atgaaaagct ttcggcttca cgtttatttg
     1321 cttggtgcgc atgttgcaat tgactagtga ttgtgacaaa gagtaagaaa tctttgaata
     1381 gttcacgtaa ttttgcgact tgttcttcat caatgagtcg tccttgctca tcaaaggctt
     1441 ggagtgaatg acccaataaa aattctgagc ttggcataat tcgtgccttt aactctggtg
     1501 aatccaatat ttggcgcaaa tgtgcttgtg cgcgtgaaga acctaacgtg ccataagacg
     1561 ctccggtaat catcactggt ttatccacaa acggaaaaat accatatgaa agccaattta
     1621 atgcgttaat caatgaagca ggaaccgcat ggtcatactc tggtgtgctg atgattacgc
     1681 catctgcttg ttcaattttt ccagcaattt ctttaacaac atttggtagt tctgtgtttt
     1741 caggtttgtt aaatagtggc agctccttga tttcgacaat ttcgatttct gcctcattta
     1801 caaaataatt tttcataaac tgtaataatt cacggtttgt tgaccgcgtt gaatttgttc
     1861 ctactaatcc aataaatttc atcttcattc tctttccttt ctaacatcga tttctgactg
     1921 ttcgcacgta ctaaattttt tcaaatcctt ttgtatacaa gaattgattc tcttttgtta
     1981 caactgctcc ctcgataccc ggctgttctt caagcgcttt caacacttct aaagaagatg
     2041 ctccaaataa acgtgtagtc cagatttctc catctacaga agaatcagac aaaatcgtca
     2101 ggcttgctac gtcattttta actggaaagc cagttttcgg atcaatgatg tgatgataca
     2161 ctgacgaacc attttgtaga taacgttcgt agattccaga ggtaactaaa gattggttgt
     2221 ttagtttaac caccgcaagc gactcttcac gtgaaccttt aggatttcgc aaaccaatac
     2281 gccaagcacc atccgaacgt cttggtgatt caccaaaaac aacgatattt cctcctaagt
     2341 taactaaagc aaacgctgca tttcgctctc ggcaatgtgt aattaattta tctgcaatgt
     2401 aaccttttgc taacgccccc aagtcaatca tcattcctgg tttggttaaa aaaacggttt
     2461 tcattttttc atctaattga atttggtgtg gatccgttag cagaagtaac tcagttatct
     2521 ctgcttttgt tggaaccaat gcatcttcaa atccaatgtg ccatttttgt acaaccggac
     2581 caatagcaat attcagataa ctatttcttg cacaactatg tgcttttcct aatttgatga
     2641 gctcaaaaag ttcaggatgt acaatcacag cttgtcgtcc agcagcttga ttgatggcca
     2701 ttaattcaga agttgcatca tgtgcgctga agcgatgttc ataaacgtgc aaccattcgc
     2761 ctaactctgc tagtaatcga ctaggctctt catgcccaac ttgtaatgta attgttgtcc
     2821 ccatcatttg ccagattttc gtttcttcga tcacctgatt cattttgtac cttctttact
     2881 tgaactacgg attacatgag taattagttt ttatttttgt tactgttttc acaaccagat
     2941 gatacgcctg ctagatttat ttgtcaaccg attttatata tttttttata taataaggta
     3001 attttaatat aaaattttat attgtaaaaa aacaaataag ggaaacaaac aacacatttt
     3061 tgtgtgttgc ttatttccct ctaactatcg ttttctattt gatggttaac ctacttaaaa
     3121 taaatactgc aacgacaaac ggagatcaag catgctttgt aacgtatttt acgataatta
     3181 cgactttatt ttattactct atcttgctgc catacccgtc tatccctaat catctttggt
     3241 gaattttttc aaagttagca taacaaaaat aagtattttt tccttagtta atcaatagta
     3301 tcattttttt catatgattt aattcatcaa ttaatttttt gtttttatta attttagcaa
     3361 aagctagtgc atcttgaatc aattcgagca cttctgcctt cgacttattt ttagcctgtg
     3421 cgttcttcgc taattgggca gcagctcgtg ctaagtaata agtcaaatgg ttatccgaac
     3481 aaatatcgat tgcgtaattt agtagtgcat cacttgtttc caaatcaccg tcttgcgcaa
     3541 aaaaaacacc acaatgaaag acgatattta acacccgcca gatatcttct gttgtacgaa
     3601 tcggatacgt ataaattttt tcaaacactc gattgaaata aacttctgct ttttccaact
     3661 ctttttctct agcatagacc attccaatac ctgtatatgc caataaacga aacaacgtgt
     3721 tggctgtttc tgccatcaac aactgatcaa aatggaacaa cacatccgta attggtgcat
     3781 tcgtaaaaat catttcaaat ccttttagat agtagtaacg tgcaatcagc tcagactcgt
     3841 caattacctt gggatcaatg gctgctaaaa tctgtgtcaa ttgttcatat tcactcgtaa
     3901 tcagtgaaaa ctctgctttt tctagctgtt catcaagttg tgtattgatc acgttcatcg
     3961 aagaaaaaag ttcaactaac ggaagattga ttcgattaca taatttgatt aaaattttta
     4021 acgaagggac ttgaccattg ttctcaaatt tacttaatgt tgcttgtgta caaattcctt
     4081 cagacacttc tttttgagat aagttggcag cctttcttgc cgtaataaat ttttcaatgt
     4141 tcattatttt ctctccaatc agacctcaca cgaaaatcat ttctgtctcg attttagcat
     4201 gttttctttg ggaatgcttt aggttctgtt gcaaagtttt aaataaagaa taaagtccct
     4261 tacggtatct atgatttaag ct
//
LOCUS       DACDXO010000119         4203 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E23605_2017
            SAMN12639989-rid9881463.denovo.125, whole genome shotgun sequence.
ACCESSION   DACDXO010000119 DACDXO010000000
VERSION     DACDXO010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639989
            Sequence Read Archive: SRR10023389
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741914.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:42:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,972
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 33 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 17 of 73
            Pseudo Genes (multiple problems)  :: 10 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4203
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639989-rid9881463.denovo.125"
                     /isolate="E23605_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(1..354)
                     /locus_tag="IWM35_002673"
                     /pseudo
     CDS             complement(1..354)
                     /locus_tag="IWM35_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348804.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(614..1162)
                     /locus_tag="IWM35_002674"
     CDS             complement(614..1162)
                     /locus_tag="IWM35_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9920485.1"
                     /translation="MADIKGLDIFGNNKGLAKIKKEAEAKKKQQAITPSENKQLEVHQ
                     VQEIKTSSNVERQSRGRTKKVGAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETN
                     MTRDELLRKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTT
                     SQIKQDTEDSLRKGWGMNNKVR"
     gene            complement(1163..2020)
                     /locus_tag="IWM35_002675"
     CDS             complement(1163..2020)
                     /locus_tag="IWM35_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAP9920486.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYSKSAMYIA
                     DWAIVVLQTQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVDQEMYKQ
                     AQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFISILKELEE
                     HENWYAEVE"
     gene            complement(2306..2592)
                     /locus_tag="IWM35_002676"
                     /pseudo
     CDS             complement(2306..2592)
                     /locus_tag="IWM35_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292679.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(2582..2911)
                     /locus_tag="IWM35_002677"
     CDS             complement(2582..2911)
                     /locus_tag="IWM35_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAP9920487.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
     gene            complement(2988..3260)
                     /locus_tag="IWM35_002678"
     CDS             complement(2988..3260)
                     /locus_tag="IWM35_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HAP9920488.1"
                     /translation="MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLPTQ
                     SFEKLKPTHEGRYSRRLNRQHRVVYKVDEDEHVVEIYSARTHYESQ"
     gene            complement(3260..3523)
                     /locus_tag="IWM35_002679"
     CDS             complement(3260..3523)
                     /locus_tag="IWM35_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAP9920489.1"
                     /translation="MAKTVLNPSSARKNFYKLLKDVNENHTEIEIISERSENNAVLIG
                     LDDWKAIKETLLLEQIGTLGVVRKREKDDSGFTNVDEIDWDNL"
     gene            complement(3629..4045)
                     /locus_tag="IWM35_002680"
     CDS             complement(3629..4045)
                     /locus_tag="IWM35_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9920490.1"
                     /translation="MEFREGAFDFQTSQQNRKFIPEDFQNLQLENMRLEDLFRLADDD
                     KYRFEVLKIKKNELLNKLYPENAIKAHFGDANHMYSWFIPKRFRGKINAGDQVKVLNR
                     NSTDVVMITDIYVEDEVIIKYMKPVIKLVNKNKGNG"
     gene            complement(4051..>4203)
                     /locus_tag="IWM35_002681"
     CDS             complement(4051..>4203)
                     /locus_tag="IWM35_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HAP9920491.1"
                     /translation="NKFYQSLRTASSTIKGMETIRGIYKKNRRNGTLFSFSVSTEIKV
                     LMGITA"
BASE COUNT         1225 a          851 c          533 g         1594 t
ORIGIN      
        1 taaatgttct tactgcctct tccacactaa tttcttcgtt caatttcact aataatactt
       61 gatctcgctt attataatcg ctttcattta tcctaagagt caaatctttt tccaccataa
      121 cttttacacc caataaattt tgcggtttat ttttctctgt tgaaattctt tgtgtagcct
      181 ttaaactgac aattagctca ttgggtacat acttatttga gagtcatcta attggctcat
      241 aatcctttct ttttggttag tatctgcagt tacttgttta gtatgagcca tgaacccaaa
      301 catactcacc ccaacaacca atcctaaata tattttcttt ttaaactttt tcatctacat
      361 ctcctcctaa tagttttatt ttagagttag ctctattact tatttactaa ctcaacaaca
      421 gattatcata cacttgttaa tgaaatcaca atatctcatt aaaacacacc ctaaaatcat
      481 aaccgttcct atgctcattt tttggatttt ttaatcacgt aaccagaaaa ataaatcccc
      541 tctatttcac gcaaatcaaa gaagaactaa gtaactaccg tttaaaggta gttacttagt
      601 tcttctttga ttattatcta actttattat tcattcccca ccctttgcgc agtgagtctt
      661 cggtatcttg ttttatctga ctggtagttc ttattatttc tccacgatca tctaattctg
      721 gaacaattgg atatttttct ctgaacaagt ctagttcttt caccacgtca ttaaacaaat
      781 cttgcttatc ttcttgcgta agattctgtt taatatactc atctaaagct tttctaagca
      841 attcatctct tgtcatatta gtctcatact tttctactaa aactctggaa gtcgcattta
      901 acaaagtcga cagttttaaa ggatgcttgg tggaataaac cttatcaaac tttctgattg
      961 gtgcgccaac tttttttgtt cttcctctac tctgtctttc aacattggag gaagttttta
     1021 tttcttgaac ttggtgcact tctaattgct tattttcaga tggtgtaatt gcttgctgct
     1081 ttttctttgc ttctgcttct ttttttattt tggcgagtcc tttgttgttc ccaaaaatat
     1141 caagtccttt gatgtcagcc aattactcca cctccgcata ccaattctcg tgttcttcca
     1201 attctttcaa aatactgata aataattcgt ggcttttgcg atcccattgt tttaagagac
     1261 ctttctttgt cttctccatc gtaatacctg atccgtcgta tcgttttaag cgcgcatttt
     1321 tgagtacaac atttttaatc aaatgttccc cataaatctc ttgggcttgt ttatacattt
     1381 cctgatctac tgcgtcaccg ctttccaaca tgaaaggaat aacaccagca acatataaat
     1441 ttgtatcaaa ttctttcaca aaaaagtcca tatactcaag atattgcatc gcatttctca
     1501 tcgattttac ttgggtctgt aagacgacga ttgcccaatc cgcaatgtac atcgcggatt
     1561 tactatatac agaaatagaa ggtgggacat ctaaatagat gacgtcaaac tcatcaaata
     1621 atggttctag cttttctttc atatatttga attgggataa tgaatcatta tattggtctt
     1681 tgatccaatc atcatacatt tcaaaggaca tgtctgctgg aagtatatag aggttattga
     1741 ttactttata gacagcctct ttaggtggaa gattgtttat caacacgtca gtcacagtat
     1801 tttcgtaaac aatcggttca ccttctccgc ctccttcttc gttagtgaag ttcatatgcg
     1861 ttaagttcat cacatcagaa gtatttcctt gcatatccaa gtcaattatc aaggttcttt
     1921 ttccttcatt aactgctgat tcaaacccta acatttgcgc gcacgtactt ttacctacgc
     1981 cacctttaaa attaccgatt acatatttct tcgctaccac gttcctacct cttttctttt
     2041 agcaatattc atttgatact attataacat atttattttt agttactacc aaaaaaaaga
     2101 tatatatgta ctaccttata attaataagt aaaaggtagt acataaataa ggaaaagctg
     2161 aaatatattg atttatagct ttcctcaaag taatgacgtt atttaattta tcgagaagca
     2221 gagaatactc gcgactttag tcatgtagac gaatcagatt ttcgataaaa aagaagccgt
     2281 ccttcttaag aacgacaaca aatcactaga ttaatttttt caactgttca taaaagtttt
     2341 catgcgttcc acataacaat actggaataa ccttgttttc tataatctcg taagctactc
     2401 tatatgttgt gcccgcatat ttgaacccac tagaccaaat acctgataaa tccccagttt
     2461 tttgttcacc cttatatgga tcaatcgcaa tttcatcata aattaaattc aaaaattttt
     2521 ctttcaatgg cttctctttt agctttttaa atacttaatg acatgttttg aaggaacgat
     2581 ttcataagcc aatttctgtt gccaactcct ctttagtcat tactttagaa ttagtaagtg
     2641 tatcttcagc aatatctcta aaggaagaat gcattttgtt ttttctattc ttaaattcta
     2701 caagaatctc atctttatcg tagccttctg caattatatc agacaaaata tcttcgctaa
     2761 aatcataaaa ttctttttgt ggttcaacaa attcataaag aataccattc tctaccaact
     2821 ttgcttcaac ttcgactcca ttaggaacat taaaatcttt aggtactgtc aacatgattg
     2881 aattgccttg ttttcttgtt tttgtaatca tttaaatccc tcatttctaa cagtatgtaa
     2941 ttacttagta aatacattat accacgagat aatgtattta caagtaatta ctgtgactcg
     3001 taatgcgtcc ttgctgaata aatttcaacc acgtgttcat cttcatctac cttataaaca
     3061 acccggtgtt gtctattcaa tcttctagaa tacctacctt catgtgtagg ttttaacttc
     3121 tcaaatgatt gggtagggag atagggatct tcctttaaag tctgaataac cttctcaaat
     3181 tgaggtttca aattagactg tttgattttc tttaaatcag ctttagccga gtttttaatc
     3241 atcacagaat agttattcat tataagttat cccaatcaat ttcatcaaca ttagtaaatc
     3301 cactatcatc tttttctcgc ttacgaacaa cacccaaagt gcctatttgc tcaagtaata
     3361 gcgtttcttt gattgctttc caatcatcca aaccaataag caccgcatta ttttcactgc
     3421 gctcactaat gatttcaatt tccgtatgat tctcatttac atctttcaac aacttataaa
     3481 aatttttccg tgctgagcta ggatttaaga ctgtttttgc catatctact ccccctctaa
     3541 tttatatctc cattctaacg tacttaatta agtacgtaaa ttcgattgtt ttgtttaaat
     3601 tggagtacca aaacagcatt gtaaagtgtt atccgtttcc tttattttta ttaacaagtt
     3661 ttatcacagg cttcatatat ttgattataa cttcatcttc tacgtagata tctgttatca
     3721 ttacgacatc tgtactattt cgattgagta ctttcacttg atctccagca ttgattttcc
     3781 ctctaaatct tttaggaatg aaccaactat acatatgatt agcatcacca aaatgtgctt
     3841 ttatcgcatt ctcaggatat aacttattca atagttcatt ctttttaatt ttcaaaactt
     3901 caaatctata cttatcatca tctgctaatc taaataaatc ttctaaacgc atgttttcta
     3961 actgtaagtt ttgaaaatct tccggtataa attttcggtt ctgttgcgaa gtttgaaaat
     4021 caaacgcgcc ctctctgaac tccaaatatc ttaggctgtt attcccatta ataccttgat
     4081 ttcagtagac accgaaaagc tgaagagcgt tccatttctt cggttctttt tatatattcc
     4141 tcgaatggtc tccatgccct taatcgtgga agaggctgta cggagacttt gataaaattt
     4201 att
//
LOCUS       DACDXO010000120         4153 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E23605_2017
            SAMN12639989-rid9881463.denovo.126, whole genome shotgun sequence.
ACCESSION   DACDXO010000120 DACDXO010000000
VERSION     DACDXO010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639989
            Sequence Read Archive: SRR10023389
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741914.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:42:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,972
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 33 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 17 of 73
            Pseudo Genes (multiple problems)  :: 10 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4153
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639989-rid9881463.denovo.126"
                     /isolate="E23605_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..106
                     /locus_tag="IWM35_002682"
     CDS             <1..106
                     /locus_tag="IWM35_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP9920492.1"
                     /translation="ASKRNNIPRKSLDYRTPLEVFLSYVSIDDLSNLI"
     gene            970..1182
                     /locus_tag="IWM35_002683"
     CDS             970..1182
                     /locus_tag="IWM35_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9920493.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPAELVS"
     gene            complement(1460..3259)
                     /locus_tag="IWM35_002684"
     CDS             complement(1460..3259)
                     /locus_tag="IWM35_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HAP9920494.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRPFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
     gene            complement(3383..3979)
                     /locus_tag="IWM35_002685"
     CDS             complement(3383..3979)
                     /locus_tag="IWM35_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAP9920495.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
BASE COUNT         1490 a          728 c          707 g         1228 t
ORIGIN      
        1 cgcatctaaa cgaaataata ttcctagaaa atcactggat tatcgaacac ctttggaagt
       61 atttttgagt tacgtaagta ttgatgatct gtctaactta atttgacaat taagattata
      121 ttaaatatta tacagataat aacggaatat tatcatcgaa acagctaaca aaattgtcat
      181 aaaaaacgag atggttgata ttagaacttg tgtttttgtt taggtctaga gataaagaga
      241 gacaatttcc aaatatatgt aatgaataca aaaagacgtt tgtcgcggat taccgttaca
      301 aacgtctttt attgattttc cgagatctca tcagtcgcca tttggaatgg ttcactcctt
      361 atctagattc gtttaatgct cggactgctt gttcgattgt aataccaaac gcaacgttat
      421 tttcgttttg tgcatcaatc gccataaaac ggttagaagt tgtatcaaaa gctacgcggt
      481 attgcatatt tgtttttgta aacgtcataa aattcatctc ctttgtaatg taatttagtt
      541 cttgaaagtt tcctttaaac tttcgaactt ataccaagtt tagcatcgta cgttttttga
      601 tgcaattaaa aagcatttat cattttcctt atttattttt tcttaaatgg tttagctatt
      661 ttcttttaat ccgataagta gcccaatcat caaggttaca aatactattg cttgaatata
      721 aatcatatgc tcacctcttt ataaataact tagcaatata aagaaaaaaa cgcacgaaca
      781 acataggaat ataaacaaac gtttttgtca caacgtctaa gatcgtgtta aaaaatagtt
      841 atctaaaaag cagaaagata ctcaaaataa aaaataaaaa aatggataaa aacgttttca
      901 aatacaaggt aatttgttat tataaaataa agaaaaaaca aaataaaaat aaagaaaaga
      961 gggaatgaaa tggtaaaagc agaagtagga agatcttata tatgcaaacc gatcggagta
     1021 tctcatgaac taatcggctg tattgaacgt acatatatca acacggctgt cgtgtatgtt
     1081 gaaagttatg accaacgaga ctatcaactt atcaatgaaa gcaagtatcg catgctcgta
     1141 aaattctgtg atattagcgc acctgctgaa ttggtttctt aaacagtatt tttgagaatt
     1201 cgcaagaact cccttataat ataaataaag acagaaggga gcgagaagat atgccaaaaa
     1261 gaaaaagttc agggaaaatg ggaattattc ttgcagtgat ctctgcaatt cctgctattt
     1321 tagctattgt tcgtgcgatg aaggaatcaa gagaaagtta agcacacatt atgagcgtca
     1381 aacaaaaaac gcatatacct gcggtggcag gttatgcgtt ttttgttgta taaagacttt
     1441 tttcattaac gatttttgat tagccgactc ccaaaacaaa atttagcagt ttatctgaat
     1501 aagtgatttc ttcatctgtt tgtttcacac tttcatttag tagtcccatc tcatcggtta
     1561 tgataccatc cacaccataa aacatcatgc gactcatagc atctgaatcg ttgattgtcc
     1621 atacatatac ttcttttccg tcttggtggg cagaattaat gaagttacgg ttgatcgtac
     1681 tatattccat tgtcagaaaa tcagcaggcg tcacaggggg cccaacaata ttgaaaggta
     1741 agatgtagcc cacataaaag tttggttctg cttcttttaa atctgaaacg gtttggtacg
     1801 tcaaactttg caaaatatga tggttctcta agataacttt gcggtattta tggacaaatc
     1861 gttcgacaag gtcatccgaa tcttttttag aggtcttgat ctcaataagt aatttttggt
     1921 ttagcttatg tgctttttcc aaataagaat caaaagaaca aacaggggct tgcactccgt
     1981 tttcagtaac tgtaagattt gtcaattctt tcaatgtaag atcctgtggt gttccattta
     2041 cccctgttaa gtgacgaaga tttgcatcat gcatcactac gaaatgacca tcttttgttt
     2101 cctgaacatc catctctaca taatcaggat gatagtctct gcttgttttt tcaagtgctt
     2161 ctaaagtatt ttgcgcccca ttttgggtac tcacacctct gtgagagaca gtcactggtt
     2221 ttgtctgaac agctgaagtc agataatcca tattgtaaag acagacgcca ataccgaaaa
     2281 aaactgcaaa aagagtcaaa ccagtgtttt tcagcgcaga gaaacgcaag ttgggggctt
     2341 gtgggataaa ccacttaggg atctcaggca agaagccttc gtcatccatg aaatcaacaa
     2401 tgatataaaa aatccctacg gtagacatca cgatattgaa taataaaatc ccttgtaaaa
     2461 gcgtcatagc aaacacagcg ctgatcaatg catagtctgg gaaaaattgt tcaaccatac
     2521 tttgtgccaa aataacaacg atatatccta aagaagaaag gagtaaaatc gtaccgccaa
     2581 tcacaataaa ttggcccgtg atggccaaaa gacgcgattt ggtcaatgac cagctttccc
     2641 gaatggctgc tcgaaaagga cgatctcgta gaatcatttc cggtaaagca aaaatcagtc
     2701 gaatcccaat atagccaagg aaaatataaa cgagtaaaaa ggaactgacg atgatccaac
     2761 gattagcaaa aataaaatcc ataatgaaag cggggatttt tatttttgac agtagatcag
     2821 aattaaaact taatccgctg atcggtagaa tcaataaaaa ataagcaagg aaaaacaaaa
     2881 aagtaatggg tctcactttc ttcagctgaa gtatcgtcaa gtgtaagagt tgcttcaaag
     2941 agattggttc tttcttttta ataaaaaaaa cactcatcaa aagaaaagta aattcgaaat
     3001 aaactaacag taaaatcgtc atcaaaacta cgattaacga gagtagaacc ccaggatgtt
     3061 gagaaaaaat tgaaggaata ttgtcataag acaaatagtc aattgcccct cggtgtaaaa
     3121 taaaacgggt cgtactgctc aataagggta aacaaataaa taaaataaag ccatgcatca
     3181 gcaagacatc acggaaataa gcttgtgttc cttttaaaaa gtcccaagca ttacctaagc
     3241 tacgtttcaa ataattcatc ctatcacctg ctcttcacat tgtagcgcaa atctaatgaa
     3301 aaaggaaagg gtgcagtgtg ttttgcagac cctttcccag atttaacatt gtcaatcaat
     3361 gactaatttt acaaaataaa tattatccaa gaagaccact tagatattga cctccataaa
     3421 tcaaggccat actatataag aaaatcgaca gtggtttagc aatcaaaatg attagtgtat
     3481 atcgtttcac tgacatgttt gtcaatccag ccatcaaaca taacgcatcg tcaggagcaa
     3541 ctggaagaaa aatagctaga gcaaacagct tctcaaaacg gttttgatta tccagccagc
     3601 caatatattt gttatatgtc ttatcgctaa tcatggaaag tatgaatggc tttccataac
     3661 gacgccctag cagaaaaatg ataatcgaac caatgcagat tccaatatag ttatagataa
     3721 atccagcata agggccaaat atcaggacac cagctgcagt actgatccct ccaggaatga
     3781 ttggaataac tacttgcagg atttggatga agacaaaaat aatcggtcct aaaatgatgg
     3841 aatcacctac taatcctctt aacgcattca aatctttgaa aacacctaaa ttaataaaat
     3901 aaatcgtcag cgcgattgat aggcctaatc cgataatgga tatgaaattt atcagttttc
     3961 gagaggcagc aatattcatt attcatcaac tccttagagg ttctttgtta actatacttt
     4021 actagaaaag caactaactg cgccattttt tgatatgaca gaaaacaaac aattttgtaa
     4081 gttcatcata agtataaaag attgatctca aaaaaacatc caactaaaga cttatttaat
     4141 aacttgtttt ttt
//
LOCUS       DACDXO010000121         4029 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E23605_2017
            SAMN12639989-rid9881463.denovo.127, whole genome shotgun sequence.
ACCESSION   DACDXO010000121 DACDXO010000000
VERSION     DACDXO010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639989
            Sequence Read Archive: SRR10023389
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4029)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741914.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:42:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,972
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 33 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 17 of 73
            Pseudo Genes (multiple problems)  :: 10 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4029
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639989-rid9881463.denovo.127"
                     /isolate="E23605_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            201..680
                     /locus_tag="IWM35_002686"
     CDS             201..680
                     /locus_tag="IWM35_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9920496.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNMDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
     gene            713..2941
                     /locus_tag="IWM35_002687"
     CDS             713..2941
                     /locus_tag="IWM35_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HAP9920497.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKERTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            3070..>4029
                     /locus_tag="IWM35_002688"
     CDS             3070..>4029
                     /locus_tag="IWM35_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HAP9920498.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKHALSAWQTSLDGIGWNALYMENHDV"
BASE COUNT         1455 a          692 c          861 g         1021 t
ORIGIN      
        1 attccaacat catcttggac tttctacatg ttactaatga gcgtgtaaaa gaacgagatg
       61 gtgtttttat ttctatcaat gaagctcttt ttctttgcaa tcctcacttc aatcctctat
      121 aatgtcgcat atagtttagt aaatgagtaa actactaatt tacttattta ctaaaaataa
      181 aaggatgagg agttaaaata atggaaatac caacaaattt aaaacataaa ccagtaatcg
      241 ttgtagaaga ctatgataaa gttgatggaa gaaatgcatt aaatatggat gcaaaagggc
      301 tttcactggg ccttgctcag tggaatgatc gtggaaaagt cgatatctca ggcaaggtat
      361 ggcgacatac tggagaaaaa tggtcacgac agtcggaaga attaccaatc acacgtattt
      421 tagatttagc gatcttagct gcgcaaggaa gtttatactt ccaagatgct tatcgttatg
      481 aaaaatttta tgatccagaa aatccagtcg tagacatcat tggattacaa gggaaccgaa
      541 tgacggtaga agtagatacc aaaaatccaa agatcgatga agacatcatt ttgtattacg
      601 atacactaca aaaagacgga gaactgattg gggaacgatt ccgtatttta aaacgtcttt
      661 tagatgagct aggatattaa tagacagaga tgatcaaaag gggaagagaa gcatggagaa
      721 tttctcacta acagaaaacc gaaccttttc acggctagaa aagcaaatga tctggaagaa
      781 accgaaaagc catcggacaa gtgcagaaga atggcggatc gcctccgaaa tcaaagggaa
      841 ttggcaagat cctgaaatga aaatcagtaa cgtacttttg gaaggagatg ccgggtcggg
      901 gaagacacaa ttagccaaag cactatctgc tgatctgcaa cttccctata caaaagtgac
      961 ctgttttgca gatatggaca aatcagatgt tttcggagcc ttattacctg tagtagattc
     1021 taataaggaa caagaccaag aattactaga agctatttac caaacagata cacttgaagc
     1081 tgtactgaca ctgatccaga accattacgg cattgatcct ttttctgcta aagaaaaatt
     1141 agcagagcta gttcaaagga tcgaagctga ttatccagat gcaatccatt acagctatta
     1201 cccttctgaa atcgttcgag cgattgaaaa aggctattta ttagagattc aagagccgac
     1261 tgtcatccga gatgcctctg ttcttgtggc acttaactca gcgctcgaac caaatgggat
     1321 gttgaattta ccgacaggaa tcattcgtcg ccatccagat tgcgtcattg tgatcactac
     1381 aaatcgaaat taccaaggaa atcgcccatt aaatgaatct ttaagagatc ggatgcaaca
     1441 tgcggaaaaa atggatcttc ctgccttaga agtcatggca gaacgtgcga tggcgaaaac
     1501 tatgatccaa aaacaagaac ttctcttaaa gatggcagag attatccgtt tgttggatga
     1561 aactgcaaaa gctaacgcca tcaaaggtgt agcaggaatg cgttcttatt tttattgggc
     1621 aaatacctat aagcaaggac aagatttact ccagtcgatt tatccaaaag ttttgtataa
     1681 attatccact gattcagatg aattggcaat tcttgaacaa gctttgtctg acagtggatt
     1741 gctactggaa cttcaggaca tattgagaaa agaaaaatgg ggtcatcctc aatctgagca
     1801 tacaaaagaa agaaccatta gcgccgaaga agcaaatgaa cgaaacatca ctgaaaatgc
     1861 tgaagaaatt ctgcgcttag atgaaaagaa atcgttgtct gctgaagaac caaatgaggg
     1921 agaagagaaa caagaagaaa caatagaaaa aaacgagatt cctcctcaaa tgcaaaaaga
     1981 acgcagtagc gatatcgaag gaaaaaatca aaaagaacaa caaacaggag atgattctca
     2041 agaaatgtct gcttctgaca tggaagcaca tgataaggaa atcaagaagc aactgaataa
     2101 agaagcgcga caaatcatga aggaaacgat tcatgaaaaa gaaggtctca tcgttcaccg
     2161 accagctttc caaggaaaaa cgccggaagc acgtgagcta cacatgcaag taatgccaat
     2221 agtagaatca cttagccggc aaattttaga gttactggat aatgaacaaa cagagacgta
     2281 ccaaaaagga aaatacgaag gtcagcggtt caatgcaagc aaagtagcat atggtgattt
     2341 gagaaatttt gacaaaaaga acccgccaca tgagcaacct tctttagcgg tagctgtacg
     2401 gatcgatgaa tctgggtcaa tgatacggga tgatcggatc gaagcggcaa aaaaagcagc
     2461 gattgccatt gccgaatttt ctaagaaagt cgatatccca ttgttgattt acggagatac
     2521 agcagaccgt tcgtcaagag aaaagacttc tttgttttct tataaagaat ttgaagacgg
     2581 atttcaatgg ttaaatgaaa aattagtcac tatgaaacca aggcaaaaca atcgtgatgg
     2641 tgctgcactt cgtttagcag cagaaaaatt aaacagacaa tccgcaacaa ccaaactgtt
     2701 gcttaatatt agtgacggtc agccaaaagc attgccggat tatacaggca aaaaagcaaa
     2761 agaagatatc cagtctgttt tgaaagaata tgaacgtcaa ggaatcctat ttgtctccgc
     2821 agctattggc caagacaaag aagaaatcaa gtcgatctac ggtgaaagtc gttttgtaga
     2881 tattactgac ttgaatgaat ttcccaagca aatgattcaa ctgattgccc gctatctatg
     2941 ataaaataaa gctcaacgaa cgcacttagc agattcgttg agcttttcgt ttctagttac
     3001 cggtaacaag gatacttcta aaatagaatc ggttataatc aagagggacc gttaaaggag
     3061 tgaaacgata tgatacaaga acaaaaatgg tggaaagaag cagtaggcta tcaaatctat
     3121 cctgctagtt tcaaagatag caatggcgat ggtcaaggag acatcaatgg gatccgtgaa
     3181 aaacttaatt atttgaaaga tcttggtatc ggctttatct ggatcaatcc gatctatcaa
     3241 tctccatttg tcgataatgg ttatgacgta agtgactatc aagatataga agagagattc
     3301 ggaacgatgg aagattttga tttattgcta aaagaagcac atgaattagg gatcaaagtg
     3361 attatggatc ttgtcatcaa ccatagttcg gatcagcatc aatggtttga agaatcgaaa
     3421 aaatctaaag aaagcccgta tcgcgattat tacatttggg tcgacggggt agatggcaaa
     3481 gaaccgaata attggacatc gattttcggc ggttctgctt gggagtattc tcatgaaact
     3541 ggacagtatt atctgcatgt ttttgcaaaa gaacaaccag acttaaattg ggaaagcgag
     3601 aaattgaaag aagaactatt caatatgatc cgatggtggt tggataaagg gatagatgga
     3661 tttcgtttag atgcgatttc tcatgtaaaa aaagatgaat attcagtaaa agccacagag
     3721 aaccctttct caccctttca aaatgtctca ggaatcgaag aacatttgac cgatttgaaa
     3781 catgtttttg aagagtacga tatcatgact gtcggagaag caagtggtgt cactgcagaa
     3841 gaaggaccac aatgggtagg gaaagacggt tatttcgata tgatattcga atttgatcat
     3901 atccatatct ggcagcaaga aaaagaaggg caattagatg tccttaaatt gaaacatgca
     3961 ttaagcgctt ggcaaacttc attggatggt atcggatgga acgctttata tatggaaaac
     4021 catgatgtt
//
LOCUS       DACDXO010000122         4008 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E23605_2017
            SAMN12639989-rid9881463.denovo.128, whole genome shotgun sequence.
ACCESSION   DACDXO010000122 DACDXO010000000
VERSION     DACDXO010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639989
            Sequence Read Archive: SRR10023389
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4008)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4008)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741914.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:42:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,972
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 33 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 17 of 73
            Pseudo Genes (multiple problems)  :: 10 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4008
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639989-rid9881463.denovo.128"
                     /isolate="E23605_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            759..1799
                     /locus_tag="IWM35_002689"
     CDS             759..1799
                     /locus_tag="IWM35_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein RepA"
                     /protein_id="HAP9920499.1"
                     /translation="MTDFKFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCRVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFVPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAEIESGISIVFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNYE
                     SSPSTITKDWLE"
     gene            2623..2955
                     /locus_tag="IWM35_002690"
     CDS             2623..2955
                     /locus_tag="IWM35_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HAP9920500.1"
                     /translation="MSYTGILSLKDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIG"
     gene            3574..3915
                     /locus_tag="IWM35_002691"
     CDS             3574..3915
                     /locus_tag="IWM35_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 domain-containing protein"
                     /protein_id="HAP9920501.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQTLTDNPIGFGLLPEDDEGNEWFKMTLMNDNGDELSVEDTWSYLSDYIVSF
                     EIIEFVADKEE"
BASE COUNT         1467 a          510 c          721 g         1310 t
ORIGIN      
        1 agattaattt ttttatgact tgtacaaatt tatctgatat tctcttatta agaactacct
       61 aatttgggta gttcttattt tttacatttt aagtagtatt taattgcaat ttggtagtat
      121 ttttttatta tcttattctt agaacggtag tacttaatat aaatataggt agtatctaat
      181 ttaagtatta atggtagtat ctaaattaac aggtagtatg taactacttt aaagtaattt
      241 ttttcagatt aacacagtag tatcttatta aataagttaa ttttaatggt agtacataat
      301 tattatatat atctgacatt ggtagtactt attgtatttt ttagtagtat ataatctatt
      361 gttttctagc ctaaagcctt gtcttataag aataatataa tatatactac tgaaataagg
      421 tagtatatat tactagtaat ggtagtatgt tttatatgca taaggtagtt cttaatttat
      481 tttttgtttt gacactggta gtacttaatt atctttaatt ttaaaactaa attttattca
      541 tacgtttttc tggtagtatt taattataaa taatatttat ttaaagtagt tcttaatgac
      601 tttaaaggta gtacataatt cattttattt ctgattttag gtagtattta attaaaaaac
      661 agttttaatg cgtttgcatt ttttatttat ctttgctatt ttcgaaaaga gtaggtaatt
      721 ttacataaat aaaacaacgt gaaagtagag gatctacaat gactgatttt aaatttttta
      781 aggcggatag agtttacaac gaattatttt atcaatttcc aaaagtcttt attgtttctg
      841 acgaatacaa aaaaatgaaa gattcaacta agattgccta tatgctttta aaagcaagat
      901 tagagatcgc aatcagcaaa cggcaaatcg atgaagaagg taatgtttat tttacttata
      961 cgacaaatga actatgtaga gtattaaact gccaaaaaca aaaagcgata gcaatcaaaa
     1021 aagagttgga atcctttggt ttattattac aaaagcagat gggatttaac aaacagttag
     1081 ggaaaaataa tcctaataga ctatatctag cagaattaaa agtctcagaa aatgatatct
     1141 acttactcga aaaatttgat agagagaata gggaaaacgt tgataaatca gagggtatga
     1201 aaatcatacc caccctcgac gaaaaatcag acgctgaatc ccttggggct caagagggta
     1261 tgaaaatcat accgtgccaa aacgttgata aatcagaggg tatgaaaatc ataccagaac
     1321 ttaataataa tatattagac actaatagac acaatataga cactgaaaaa gaccgcctac
     1381 aagatcaatt gttgttagac aattttgaga caattatgac aaacgacagc attgctacgt
     1441 ttgtccctga acgagtatta aatttgataa aaacattttc ttcaagttac agtgaagctc
     1501 aaaaaaccgt ccagactatt cataatgcaa agaaaaaagc tgaaatagaa agtggtattt
     1561 cgatagtttt tgaagaactc gatagttatt atgtcaatgc agaacaagaa ttatacacga
     1621 cactgttaaa agcctatcaa aaattaaaaa ccgaaaaagt cgaaaatatc cagaacctga
     1681 tttttgtcta tgtaaaaaat tggtttatcg aaaaaccaat agctgctaaa gtatcaagtg
     1741 aaaaacgttt gaattatgaa agctccccaa gcactattac gaaagactgg ttagagtgaa
     1801 actttactga caaagtgatt gttgcttcat ggagtaacat taaaattaaa aattttttat
     1861 tattctaatt atattacgaa tattacaaaa taatagctat tttaatagaa tttataagtt
     1921 aaaatattat gataaaaatc acaaacgtag ccttttacgt cataagtgtt aaaagaaata
     1981 attaatttca aatatagaaa agggaacgta tgtttgcata ctcctttttt ttgagttata
     2041 ataatacgcg aagagagata atttcattat ttaaacaagc taaaaatctt gatattactt
     2101 cttttcaggc aatacgaata ttattcgctt acattttttg tataaattta attgtctgct
     2161 atgcttgtga tgaattgatt ttcgttctgc acttttggat gataaatttg gacaattgaa
     2221 aatacaaaaa aaagaaaggc ggtatgaact cggaaggctg gtaaccttcg tcagcgttca
     2281 tacacggcat taagatttgg cccttaatgc gttaaccttt cagtgttacg gtattacccg
     2341 caatggattt agtataacag atattcaaaa gtgataccac tttttttggc tgtgttaagg
     2401 ttaaaaatac gtgatattac gatgaatcta tgcctaagca caatgttata aaaatgtccg
     2461 tgtggctctt tgccgtatga cgaacgctga aattagaggg aacgcatgtt tggatgcgtt
     2521 ccctcttttt tgtttgtcat gaacgaaaat caattctcta tttttgaaaa ataacaagaa
     2581 aaacttgtta aaaatattta aagaaattgg gtggtttatt ttatgtcata tacaggaatt
     2641 ttgagtctca aagatatttg tcactacggt aaacggtgta ccgctacaga gaaaatcacg
     2701 aagaaactat caactggcca aagtaaaacc gttgtgcagt gtaagaaata tataatccag
     2761 aaagacaaag tctcagagga aatgatttat tacattggga aacaaaagca aataatttta
     2821 aaagatccta tccccttaaa agagctatat ccaacaatca aacatgttta tgaccaaaac
     2881 ggtgtactga ttggtcgaag aaaaaatgga gtcttgcgtt gtaccgcaaa agggatgggg
     2941 cgtttgatcg gctgaattga tcattctcat aacttgtatt ttatcgtttt tcgcttgctt
     3001 gaaaatattg tcgagacttc taaaggaact tagagtcact agaaaaataa ggagtagact
     3061 tgatctatca acaattccct tcggggagtg acggggtagg ttttaacgat tggtaacgta
     3121 aaaaattggt caatcgagta aatgcgcttg tttggatagt ggtgaaatga atttgtgtaa
     3181 ataataagcg cagagtaaac aagagggaat ttagaagtta gacaagttag aatagcgtct
     3241 agattaatca tgagcagcta ctaagggatc tagactttga cgcaaactca tggaataagg
     3301 agtaggtctt ctcgtaatgg ggaggacgca caagaaattg tgtcgtgtgc tgcagtacat
     3361 gtagatactt gcgaggacgt tgattattgg atcaatcgag catttaccgc tgtctactag
     3421 ggtttctcgt ttcggacgcc ataaacgagt cgaaattttt tatgtatttt taagtaaacg
     3481 aaaaacgata aaatatgaac aagtcctgaa taaagtgaaa aaattgatat aaaacataga
     3541 gaaaatgaaa tttacgtaat ggaggaatga caaatgaaaa attatgatcc gaatatacga
     3601 ttgggcacac atactattaa ggtttctttc caacgttggg attacaaagg atttgtgacg
     3661 tttagaagag gcggaaattg taaaggatta gatgttttag cattagacga ggatgattta
     3721 tacgatcaaa cattgactga taatccaatt ggattcggct tgctacctga agatgatgaa
     3781 ggtaatgaat ggtttaaaat gacgcttatg aatgataatg gagacgaatt atctgttgaa
     3841 gatacttggt catatttgag tgattatatt gtcagctttg aaatcattga atttgtagcg
     3901 gataaagaag aataaaaact ggaggaataa attttacaga taaaggctta gaaggagtca
     3961 cgatgattaa taaaaaggta gtattacctt aggcattgag taaggaat
//
LOCUS       DACDXO010000123         3866 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E23605_2017
            SAMN12639989-rid9881463.denovo.129, whole genome shotgun sequence.
ACCESSION   DACDXO010000123 DACDXO010000000
VERSION     DACDXO010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639989
            Sequence Read Archive: SRR10023389
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3866)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3866)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741914.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:42:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,972
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 33 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 17 of 73
            Pseudo Genes (multiple problems)  :: 10 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3866
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639989-rid9881463.denovo.129"
                     /isolate="E23605_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..135
                     /locus_tag="IWM35_002692"
     CDS             <1..135
                     /locus_tag="IWM35_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP9920502.1"
                     /translation="FNQVNQGFISSVASKRNHIPRKSLNYQTPLEVFLSYVNGKFCLA
                     "
     gene            complement(313..2310)
                     /gene="tkt"
                     /locus_tag="IWM35_002693"
     CDS             complement(313..2310)
                     /gene="tkt"
                     /locus_tag="IWM35_002693"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HAP9920503.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2412..2663)
                     /locus_tag="IWM35_002694"
     CDS             complement(2412..2663)
                     /locus_tag="IWM35_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HAP9920504.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2810..3433
                     /gene="lexA"
                     /locus_tag="IWM35_002695"
     CDS             2810..3433
                     /gene="lexA"
                     /locus_tag="IWM35_002695"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HAP9920505.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
     gene            3613..3831
                     /locus_tag="IWM35_002696"
     CDS             3613..3831
                     /locus_tag="IWM35_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9920506.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
BASE COUNT         1082 a          827 c          707 g         1250 t
ORIGIN      
        1 ttcaaccaag tcaatcaagg attcatctca tccgttgcgt ccaaaagaaa ccatatccct
       61 agaaaatcac taaattacca aacaccatta gaagtttttt tgagttacgt aaatggaaag
      121 ttttgtctcg cttaatttga caaataatat tctcaagaaa cacaaactaa tccgaagaat
      181 tctttgtttt gtcatttaaa tgtaacatag ccgaaaacga aaagtaatat tgatttattc
      241 ttctaacaca taaagaacag ctgtctccaa cttgcagaca gctgttctta tcagtcaaac
      301 aattgcttgg atctatgata gttgattgta cgtattcacg acattttcta ctgtaaatcc
      361 aaattcttct aaaattttag tgccaggggc agatgcaccg aaatgatcga ttgtaatcgt
      421 tgcgccctct gttcctacgt agcgttccca tccaaatgat gcagcagctt cgatagccac
      481 acgtttcttc actgatttag gaagtacact ttctttatat tcagaagatt gtttttcaaa
      541 gaggtcaaag cttggcatag aaacaacaga aacatctttg cctttttcag ccaattcttt
      601 ttgagctttc acagccaaat caacttcaga acctgttgca atcaaaatac cttctggtgt
      661 ttctccttgt gatggagaaa gaacatacgc gccttttttc accatttcat ctgctacttc
      721 ttttgtagaa ggcaatactg gaaggttttg acgactcaat acaaggatag taggcgcatc
      781 tgttgtctcc atcgctactt tccaagcagc acgtgtttca tttccgtctg ccggacgaat
      841 cacttgaaca ccaggcatgc agcgaacaga tgctagttgt tcaatcggtt cgtgcgttgg
      901 tccgtcttct ccgacagcaa ccgagtcatg tgttaagacg taaacaacag gagtatgttg
      961 gattgctgct aaacgaactg ctggtcttag ataatcgaca aagacaaaga atgttccacc
     1021 gtagatacga gttccgccat gaagttggat accattcata gccgaagcca ttgcaaattc
     1081 gcggacaccg aaccagatgt tgcgtccttc ataatgttca ggagtgaaat ctttatctgc
     1141 agccaccatt gtattattgg aacctgacaa gtccgctgaa ccaccccaga agcttggtat
     1201 agctttcgat aattcttgga tgacttcttt acttgaaaca cggcttgctt gactgcttcc
     1261 aacttcataa gttggcaatt ctgcatccca gttttctggt agtttgccat caaaagcatc
     1321 ttcaaattgt tgtgctaatt caggataagc ttttttataa ttggcaaaca tttcattcca
     1381 tgcatcttca gctttttgtc cttcttctat catcgtttga tggaagcggg cagctacttc
     1441 ttctggaaca gtgaaatctg gatactccca accataaact tctttagcca tcttgatgcc
     1501 atcttcacca agaggagcac catgaacagc agatgttcct tctttaggag caccatagcc
     1561 gatgactgtt tttacttcga tcaatgttgg tttgtctgtt tcagcttttg cttcttcaat
     1621 cgctttagag attgcttcta ggtcatttcc gtctttcact aagatatgtt gccagccata
     1681 tgcttcatag cgagcaccaa cgttttctgt gaaagctttt gaagttggac cgtctagtga
     1741 aatatcgttt gaatcgtata aaacgatcaa tttccctagt ttcatatgac ctgccatcga
     1801 acttgcttct tgagataccc cttccatcaa gtctccgtca ccgcatattg catacgtata
     1861 gtgatccatc acattaaagt tttctttgtt atacgttgct gcaaggtgcg cttctgccat
     1921 tgccataccg acagccattg caatcccttg tcctaatggc cctgtagtgg cttctacacc
     1981 atctgtatga tgtacttcag gatgtcctgg tgttttagaa tcccattgac ggaattgttt
     2041 caaatcatcg attgtcactt gatagccagc taaatgaagc aagctataca acattgctga
     2101 cccatgtcct gccgaaagaa cgaaacggtc gcggtcagcc cagttttttg atgtcgttgg
     2161 atttactttc aggtgttttg tccataacgc atatgccata ggtgctgcgc ccatcggcaa
     2221 tccaggatgt cctgagtttg ctttttggac tgcttcaata cttaatgtac gaattgtgtt
     2281 aacgccaagt tggtcaattt tgtcaaacaa aataacccct cctattaatt taggtttttt
     2341 ttacctttat ttcttacact ttcattctat aagatgtcac acgaaaaagc aatctttttc
     2401 agtatttttt actaaaagtt gtcatttcgg ttgtgtaagc cttttttctt ttggatttct
     2461 tttaattttt ccggggtcac gtcattgccc tcaggatcga cgactttcat tccttcgata
     2521 tgatgacgca taccggaacg aaaagcttcc aaatactctt tgcgcaagtt gatttgttct
     2581 tgttcttcgg cttttgagag accattttcc ttctttttac gtgcaagttc attgatccgc
     2641 tctatttttt caggtgacag catgctcagc cctcctttta ctctccttca tattatataa
     2701 aaactgaaaa aaaaacaact agataaacta aataaaatac gaacattcgt ttgatttagc
     2761 gctttttata tggtactatt tatttatcaa gtgaaagaag gtgtaccttg tggcccgaca
     2821 aactgaatca agacaaatcg aagtcttacg atatatccat gaacaagtaa cccaaaaagg
     2881 atacccgccg actgtccgtg aaatcgggga agctgttcag ctttcttcta cttctactgt
     2941 tcacggtcat cttgcacggc ttgaaaaaaa agggtttatc caaagagatc cgacgaaacc
     3001 tcgtgcaatc gagttgacaa aacaaggttt agaaaagatt gggattcgtc caacaactat
     3061 tccagtactt ggcgtcgtaa cagctggaga acccatttta gcagtagagg atgcttcaga
     3121 cttctttcca atccctccta atctttcttc cgaagaaggc agtctcttta tgctgacaat
     3181 tcgtggagat agtatgatca atgctgggat attggatggt gacaacgtga tcgttcggaa
     3241 gcaagaaact gctcaaaatg gcgatattgt cattgcaatg actgccgagg acgaagcgac
     3301 ttgcaaacga ttctataaag aaaaaaactt tttccgtctg cagcctgaaa acgacttgct
     3361 cgaaccaatc atcttagatc aagtatctat attaggacga gtagtaggac tttatcgtag
     3421 tcatatctat taattgctac taattaaaag aaaacttttt aaaaagagtt tgagacgttg
     3481 gtctcaaact cttttttcgc ccgggttctt cttctttcaa atttttatca cttccctctt
     3541 tacaaaccga acaaatattc gtatactaaa tatacgaaca attgttcgca taaattaaag
     3601 aggtgagaat tcatgaaatc aatgagacgt cttggcttag tattaactgt tttgttttta
     3661 ggtattatta ttggtaattt cggcatacgt ccagctttac ttgtgtttgt tccgttattt
     3721 actttgtggt tcatgttgtg ggatgaaaag aagtaccgtc aatctgagcg gcgcagaatt
     3781 catgaagatc atttctatca tgagccctac tacagatcca ggcaatcata ataaggattg
     3841 actgctaatt attcaaaaga caagaa
//
LOCUS       DACDXO010000124         3648 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E23605_2017
            SAMN12639989-rid9881463.denovo.132, whole genome shotgun sequence.
ACCESSION   DACDXO010000124 DACDXO010000000
VERSION     DACDXO010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639989
            Sequence Read Archive: SRR10023389
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3648)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3648)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741914.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:42:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,972
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 33 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 17 of 73
            Pseudo Genes (multiple problems)  :: 10 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3648
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639989-rid9881463.denovo.132"
                     /isolate="E23605_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..235
                     /locus_tag="IWM35_002697"
     CDS             <1..235
                     /locus_tag="IWM35_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAP9920507.1"
                     /translation="SGKSLEALELEQEESIRFQNCSLFPLYHGSAKSNIGIDNLIEVI
                     TNKFYSSTHRGPSELCGNVFKIEYTKKRQRLAY"
     gene            410..706
                     /locus_tag="IWM35_002698"
     CDS             410..706
                     /locus_tag="IWM35_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9920508.1"
                     /translation="MQIGVLKWLQFTKQKGVHFPMQFLEPKNKNAKSVDWEISEQVRV
                     IVKQYAEYAERTESEAVDEFLLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            713..2665
                     /locus_tag="IWM35_002699"
     CDS             713..2665
                     /locus_tag="IWM35_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HAP9920509.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(2737..3234)
                     /gene="dfrG"
                     /locus_tag="IWM35_002700"
     CDS             complement(2737..3234)
                     /gene="dfrG"
                     /locus_tag="IWM35_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HAP9920510.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            3464..>3648
                     /locus_tag="IWM35_002701"
     CDS             3464..>3648
                     /locus_tag="IWM35_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HAP9920511.1"
                     /translation="MKYILSFCTPKLNYLLAYIRLYSGVLHLRDSVRVSEKEKIKVTE
                     MYTSINGELCKIDRAYSG"
BASE COUNT         1297 a          589 c          633 g         1129 t
ORIGIN      
        1 gtccggtaaa tcattagaag cattggaact cgaacaagag gaaagcataa gatttcagaa
       61 ttgttctctg ttccctcttt atcatggaag tgcaaaaagt aatataggga ttgataacct
      121 tatagaagtt attactaata aattttattc atcaacacat cgaggtccgt ctgaactttg
      181 cggaaatgtt ttcaaaattg aatatacaaa aaaaagacaa cgtcttgcat attaagtagt
      241 tcagttttgg agtacaaaaa gtgaaaatgt aaacctaaat gagtatcaaa ttgcaactga
      301 atttgatact cgttttttat tgttaaatca acgtttgttg ttaatttaca ccacaaaatg
      361 taagttaaaa tgtatataaa aatgcgaacg tgaaataatt attgtaggaa tgcaaattgg
      421 tgtgttaaaa tggttacaat ttactaagca aaaaggggtg cattttccta tgcaatttct
      481 tgagccaaaa aataagaacg ctaagtctgt ggattgggaa atatcagagc aagtaagggt
      541 gatagttaag caatacgctg aatatgcaga gcgtactgaa agcgaagcag tggatgaatt
      601 cttattgaac attttagacg ataagaagtt tattgaatgg attgccaaca agcgaagtaa
      661 taagcggatt gttgaaaaaa tgggtatcaa agatagagtg ggttgataga caatgaccaa
      721 gcttaaaaga ttagccacga aggaagatga aattgtagat gttaaaattc cgatttcaaa
      781 cgaagaatta aaagataggg ctaaacaata tgatttatta actccaaaac gatttgcaac
      841 aaggtataac aaaatgttgt tcttgccaac gtcttttaaa tggaacggtt ctgaatatcc
      901 cattcaatat aattactgca tcaatccgtt ttgttgtaat tttgggaaag aacagcacaa
      961 attcaaagat gtaaaaggga aaccaagtcg atataaaatg actggtagtt ccaaagataa
     1021 aggtcataaa ggtatgtact gcaatgacaa tcctattggc agaggtgtat cgcaaaactg
     1081 tacagtaacc cctttatcta attggtctgt tgttgaagaa attaaacgcc ttatagaaat
     1141 caatagcatc caagatgttg agccagatta tcaatttcat aaagaaggct gctcggaaga
     1201 agaatcaacc ccattcaatg agccgaagca attttacaaa agggggaaaa gtaggggcaa
     1261 atctcaacgc tatcaatgta aggcgtgtaa gaagttcact aatgttctac caaaaagaga
     1321 agaaaccact acttatcacc aacaaaagaa caccattctt ccaatgttcg ctaagatggt
     1381 tgttggaagg gtttcagtca gtcgtacttg tgatatttta ggaattggag ttggaaccta
     1441 ctatcataag ctggagtggc tgtataggcg ttgtttggag tttttagaac gatatgagac
     1501 acaacccctt caaacaaaga aatttaacga gatgtggctt aatacggaca agatgcacta
     1561 ctacctaaat aacgtccgta agaaaggaca aggttcaaag aaatatacgg ggtttgaaga
     1621 ccttaatatg caaacgtata tagttgtatc tgctgaggtc ttatcaagat atgtgttccg
     1681 ttctgatgtt gcctatgatt ggaatatttc aatggatgaa ttaaatgaag atacaagaaa
     1741 attcaaagaa gaccatctca atacttttag tcgtaaaaat gataggttag attggtctta
     1801 ttatccacaa gaaccatccg ctaacgatag cgaaaatagg aatgcttatc ttcacgaatt
     1861 agggaaaatt actaatagga gtagatttgt cgatggttta aatgttgatg ctccttatac
     1921 tacaactgct cactattggt taattaaaca aatggttaat gccgacgaat ggcgaatgat
     1981 tagtgatgat gacttctcta ttagaaacgc cttttataga gtattcacga aagaattaag
     2041 gttgagcgat gcacatcatt ttatttgcca agttaacaag acgaaaagca gaaaacaatg
     2101 cttaaaagaa ttcggacaag caaaagcaga actgctcgat tggggagata tcagaggatt
     2161 caaaacaaag tttctacgga ctttggcatc tcattatcta actgaattac taacaagtca
     2221 tcaattccac gaagaggcta ttagtaaaga tggagaaaga tatcgcaaat atgccgataa
     2281 ccctatcaaa catccattag ccactaagga taaaggattt tattcagttg attgtagaac
     2341 ggatttatct gctctggaac caaacgaaat cgcaaagatg ctattaaatg ttaatgacca
     2401 ttcgaccaat agctttattc aacagataag acgttatata tcatctcttg aaagaccatt
     2461 aacaacagct cgtggtgata agaagagcta tatttacgct aacttcaatc cgaagtatgc
     2521 tcaatttgcg attacaattc taagaactta ctacaacttt tgtcgccctt ttaaatcagc
     2581 ggataaaaag gttttgacac ctgctcaaag attagggatt acagataaac aatttgattg
     2641 gaaagatata atttatttca aatgacaaaa agagcttgga gtaatcccaa gctcttgtgc
     2701 tttatcttta ctgttcttct tcaattaacg cacccgttaa ctcaataagt tttttctttc
     2761 atatacatga aaatagtagt tatacggatt tttatcattc tttatccctt tttgggcaaa
     2821 tacctcattc cattcctcat aattcacttc tggaaaaaaa gtatctcctt cgaattcatg
     2881 atgtattttt gtgatgtaca ttttctcaac ataagggaaa aacaaattat aaatctgttc
     2941 tcctccgaaa ataaaaattt cttcttcgtt tttacataac tcaaaaacat cttctattga
     3001 atgaacaatt tcacaaccat taaaggtaaa ccccttatct ctcgtcagaa taatatttct
     3061 tctgtcaggt aaggctcttc cgattgattc aaggttcttc ctacctaata ttatcggatg
     3121 tccctttgta gtatttttaa catattccca gtccttggga atcctccaag gaatgtcatt
     3181 ctctttgcca atcactctat tcttatccat cgcagcaatc aaagaaactt tcatttataa
     3241 cacagccttt ttaactaatt taccatctaa tttcttattt ctatcaaaaa accttttctc
     3301 attatcctgc ccgttagttg aataagcaat tttttctata agttatatta tcataatacc
     3361 acaaaatcac aaattttcat tcaattatca ttttcaattg cattttataa atcattttgt
     3421 atgtaatttg gtatgtaagt taaaatgaaa aaagtagtcc attttgaagt acattttgtc
     3481 attttgtact ccaaaactga actacttact tgcatatata cgcctttata gtggagtact
     3541 acatttacga gattcggtta gagtatcaga aaaagaaaaa ataaaagtta cagaaatgta
     3601 tacttcaata aatggtgaat tatgtaagat tgatagagct tattctgg
//
LOCUS       DACDXO010000125         3568 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E23605_2017
            SAMN12639989-rid9881463.denovo.133, whole genome shotgun sequence.
ACCESSION   DACDXO010000125 DACDXO010000000
VERSION     DACDXO010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639989
            Sequence Read Archive: SRR10023389
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741914.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:42:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,972
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 33 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 17 of 73
            Pseudo Genes (multiple problems)  :: 10 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3568
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639989-rid9881463.denovo.133"
                     /isolate="E23605_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..683
                     /locus_tag="IWM35_002702"
     CDS             <1..683
                     /locus_tag="IWM35_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019117172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetracycline resistance protein"
                     /protein_id="HAP9920512.1"
                     /translation="KEPTVIYMERPLKKAEYTIHIEVPPNPFWASIGLSVAQLPLGSG
                     VQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYSPVSTPAD
                     FRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIVDTQLKNN
                     EVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRPNSRIDKV
                     RYMFNKIT"
     gene            802..969
                     /locus_tag="IWM35_002703"
     CDS             802..969
                     /locus_tag="IWM35_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP9920513.1"
                     /translation="MCPVCGNKTRLKIREDTELKKFPLYCPKCRQENLIEIKQFKVTV
                     ITEPDAKTQSR"
     gene            complement(1029..1382)
                     /locus_tag="IWM35_002704"
     CDS             complement(1029..1382)
                     /locus_tag="IWM35_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9920514.1"
                     /translation="MRKKEDKYDFRAFGLAIKEARLKRGLTREQVGALIEIDPRYLTN
                     IENKGQHPSIQVLYDLVSLLHVSVDEFFLPANNLVKSTRRLQIEKYMDSFTDKELSLM
                     ESLASGINEARNIED"
     gene            1887..2210
                     /locus_tag="IWM35_002705"
     CDS             1887..2210
                     /locus_tag="IWM35_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002414693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAP9920515.1"
                     /translation="MKPSSFQTTIENQFDYICKRAMEDERKNYMLYLSRIAKREVSFS
                     DVGDYLVSQFATTDNYSTDFQIFTLNGLSVGVENDLLSEALRELPDKKREILLLFYLW
                     EFRYF"
     gene            2189..2518
                     /locus_tag="IWM35_002706"
     CDS             2189..2518
                     /locus_tag="IWM35_002706"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP9920516.1"
                     /translation="MGISLFLELPAIVSYWVVTVMCFVAMGVSTMFVVQIYTLVQTQT
                     PPQLVGKIMATLVAVAMCGQPIGQMVYGILFDVFIQETWVVMFVASIASIMISIYSKR
                     IFQKLEI"
     gene            2745..3164
                     /locus_tag="IWM35_002707"
     CDS             2745..3164
                     /locus_tag="IWM35_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009269612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAP9920517.1"
                     /translation="MRTSSFEHIVRLQFNSLMLIVIKNKVKSRNRQLARHSKREVLFC
                     EIAETKQVERGTIDTCFYDCVSFKVLHFTIYVSDETLGTVLHRLSEKQRSAILLRYFQ
                     GMNDRKISELYHVSRSAICSRRTRGLRKLKMLLSEGK"
     gene            3169..3408
                     /locus_tag="IWM35_002708"
     CDS             3169..3408
                     /locus_tag="IWM35_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008788617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9920518.1"
                     /translation="MSKDYGYPSFELICKASSGDEMAVKEILKFYDGYISKLCLRPFY
                     HSESGKIIMQVDEELKGEIHTDMMKAILKFEIGVK"
BASE COUNT         1164 a          541 c          700 g         1163 t
ORIGIN      
        1 taaaagagcc tacagtcatt tatatggaaa gaccgttaaa aaaagcagag tataccattc
       61 acatcgaagt tccaccgaat cctttctggg cttccattgg tctatctgta gcacagcttc
      121 cattagggag cggagtacag tatgagagct cggtttctct tggatactta aatcaatcgt
      181 ttcaaaatgc agttatggag gggatacgct atggctgtga acaaggattg tatggttgga
      241 atgtgacgga ctgtaaaatc tgttttaagt atggcttata ctatagccct gttagtaccc
      301 cagcagattt tcggatgctt gctcctattg tattggaaca agtcttaaaa aaagctggaa
      361 cagaattgtt agagccatat cttagtttta aaatttatgc gccacaggaa tatctttcac
      421 gagcatacaa cgatgctcct aaatattgtg cgaacatcgt agacactcaa ttgaaaaata
      481 atgaggtcat tcttagtgga gaaatccctg ctcggtgtat tcaagaatat cgtagtgatt
      541 taactttctt tacaaatgga cgtagtgttt gtttaacaga gttaaaaggg taccatgtta
      601 ctaccggtga acctgtttgc cagccccgtc gtccaaatag tcggatagat aaagtacgat
      661 atatgttcaa taaaataact tagtgtattt tatgttgtta tataaatatg gtttcttgtt
      721 aaataagatg aaatattttt taataaagat ttgaattaaa gtgtaaagga ggagatagtt
      781 attataaact acaagtggat attgtgtcct gtatgtggaa ataaaacacg attaaagata
      841 agggaagata ctgaattaaa aaaattcccc ctctattgtc cgaaatgcag acaagaaaat
      901 ttaattgaaa taaagcagtt caaagtaact gtgattacag agccagacgc aaagacgcag
      961 agccgataaa atgagattaa tacaatctca ttttatcggc tctttccgtt atgtatggat
     1021 tcttttaatt agtcttcgat gtttcttgct tcgttgatac cgctggctaa agattccatt
     1081 aaggatagtt ctttgtctgt aaagctatcc atgtatttct ctatctgtaa tcgtcgggtg
     1141 ctttttacca agttattagc aggtaagaaa aattcatcaa cggaaacatg aagtaacgat
     1201 acaaggtcat aaagaacttg tatgctgggg tgttgccctt tattttcaat attagttaag
     1261 taccgtgggt caatttcaat caatgctccc acttgttcac gagttaaacc tcgtttcaat
     1321 cgagcttctt taatggctaa accaaaggct ctaaaatcat atttatcttc ttttttacgc
     1381 atagtagacc acctctatac attttattgt tcctactgaa ttaaaaacag gtatagaaaa
     1441 acgtgttata tggtttatag gtttatattt aataaaaagc actactaaac gccaataaaa
     1501 aaaaccgtta tatggtagtg ctatttacgc tgttaaaata ttgtatatta cttccaaatg
     1561 gcggtttgtt ggaggtcaac gtcgccatga agtacatcat atacaataaa tttccttaca
     1621 ttgggttctt gtcaaaaaaa gtcgtctatc tgcaatagat aagtacgtcc accaatgtgg
     1681 ttttataaat catatagata gaataacaga agcatgtaaa cagagaaata aatctgttta
     1741 tatgcttttt tggctattca gaactttttt acaaagttta tttatcagta atgcaacaaa
     1801 tccccctttc acattgggac taagagtgaa aggagataaa cgagcaaggc tcacttcctt
     1861 tcctagacag aaagggggtg agaaacatga aaccatcttc ttttcagacc acaatagaaa
     1921 atcagtttga ctatatctgt aaacgtgcta tggaagacga gcgaaagaat tatatgcttt
     1981 atctttcaag gattgcaaag cgtgaggtgt ccttttcgga tgttggcgat tatcttgtta
     2041 gccagtttgc gacaacagat aactattcaa ctgactttca gatttttaca ctcaatgggt
     2101 tatcagtagg cgttgaaaat gatttgttga gtgaagcatt acgtgagttg ccagacaaga
     2161 aacgtgaaat tctactgctg ttttacttat gggaatttcg ctatttttag aattgcctgc
     2221 aatagtttcc tactgggtag ttacagtgat gtgctttgtg gctatgggtg tatctactat
     2281 gtttgttgtt caaatttaca ctcttgttca gacacaaaca ccaccgcaat tagtaggaaa
     2341 gataatggct acgctggtag cagttgctat gtgcggtcaa cctattggac aaatggttta
     2401 cggtatttta ttcgatgtat ttatacaaga aacatgggtt gttatgtttg tagcttctat
     2461 tgcttcaatc atgatttcaa tatattcaaa aagaattttt caaaagttag agatataata
     2521 caaggggtta aatagtatgt taggctaaaa acaatatata aaagaaaatt tgaaatctgc
     2581 taaaaggata gtgttataaa agaaagagta atattgcagt atttaaggct cgcacaaata
     2641 tatgttttgt gtgggctttt ttcttaccat ttttctgtat ttctaaaggg tataaacggt
     2701 attccgttta tatatttcag cattagcaga aagggggtga gattatgagg acatcttctt
     2761 tcgagcatat cgtcagatta cagttcaatt ctttaatgct gattgtcatt aagaacaaag
     2821 tgaaaagtcg aaacagacag cttgcaagac attctaaacg tgaagtattg ttttgtgaaa
     2881 tagccgaaac gaaacaagtt gaacgtggaa cgattgatac ctgtttttat gattgtgtat
     2941 catttaaagt cttgcacttt acaatttatg tatctgatga aacacttggt acagttttac
     3001 atagactgtc agaaaaacag cgtagtgcga ttttattacg ttattttcaa ggaatgaacg
     3061 acagaaaaat ttcagaactg taccatgtat cacgttctgc tatttgcagt agaagaacta
     3121 gaggactaag gaagttaaaa atgcttttga gcgaagggaa gtaaagaaat gagtaaagat
     3181 tatggctatc cctcttttga attgatttgc aaagcgtcaa gcggagatga aatggcagtt
     3241 aaagagattt tgaagtttta tgacggttat atttctaaat tatgcttgcg tcctttttac
     3301 cactctgaaa gtggtaaaat cattatgcag gttgatgaag aactaaaagg agaaattcat
     3361 acagacatga tgaaagcaat attgaaattt gaaatcggag tgaagtaatc aactgtataa
     3421 aaaaatcggg agatacacag tttcgtgcta tctcccttag tagttttacg gtcatatcaa
     3481 ggctcattca atcgtggtaa attcgtggta agatgagtgt ttttctatac ttcaaaatgc
     3541 ttgaatgtat agtgtttatg cggataat
//
LOCUS       DACDXO010000126         3128 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E23605_2017
            SAMN12639989-rid9881463.denovo.134, whole genome shotgun sequence.
ACCESSION   DACDXO010000126 DACDXO010000000
VERSION     DACDXO010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639989
            Sequence Read Archive: SRR10023389
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741914.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:42:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,972
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 33 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 17 of 73
            Pseudo Genes (multiple problems)  :: 10 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3128
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639989-rid9881463.denovo.134"
                     /isolate="E23605_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            103..1347
                     /locus_tag="IWM35_002709"
                     /pseudo
     CDS             103..1347
                     /locus_tag="IWM35_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729806.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
     gene            1360..1992
                     /locus_tag="IWM35_002710"
     CDS             1360..1992
                     /locus_tag="IWM35_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP9920519.1"
                     /translation="MTMGVIFDMDGVLIDSETFYFERRMNFFQELEIDPGSNQIDDYV
                     GKTEAAIWETLVPEDKSLREELYPKYVDYRKTHPIDFAKALRKEVKPLLDFLKKRDIP
                     LALASSSPAVEIESMLIQCELTEYFSFVISGESLKQSKPHPEIYLKAKQQLDCDFFIA
                     VEDSTIGIKSAKAANIYTAALKQPMQIDQSEADIQIESLQELEQIVLEKG"
     gene            2197..2424
                     /locus_tag="IWM35_002711"
     CDS             2197..2424
                     /locus_tag="IWM35_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009320234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HAP9920520.1"
                     /translation="MLQQNQNTNSYQAAILTAVNLGGDTDTTAAVTGALAGILYGGEV
                     IPENWRKSLQNDQLLKTISQRFCDTVLSQRI"
     gene            2493..2759
                     /locus_tag="IWM35_002712"
     CDS             2493..2759
                     /locus_tag="IWM35_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HAP9920521.1"
                     /translation="MLVQMMNGGRIMSQSTITFRIPDDEKELVLEYAKAHNSSLTELY
                     RNAVLDKIEDEIDLRTLRSAIEVSKEKKESGISQEEMEKLLNEL"
     gene            2749..3018
                     /locus_tag="IWM35_002713"
     CDS             2749..3018
                     /locus_tag="IWM35_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAP9920522.1"
                     /translation="MNYKVIYMPQAQKQLKKMDRNQAKIIVAWIRKNLENTTDPRQHG
                     KGLTGNKSGEWRYRIGNYRILANICDSEIEIQIFSIGHRSTIYKR"
BASE COUNT         1101 a          466 c          617 g          944 t
ORIGIN      
        1 tgaaattgct ttcctttaaa atgactcatc gtcattcctc ctgctatctt tttctattat
       61 tctaccttat ctgatagtag atttaaaact ttgcaacaga accagaaaac caaagatcag
      121 cttaacgaat tttttgctaa tgttcttgaa accccttatt ggtatgcaaa aaaaatctta
      181 agccttttat ctgagtgttt agatcaacct gtgtatgaag aggacatttt ctttattacg
      241 ctatattttt atagtttgaa tgtcacgaat gttaaaacag gtattaagag tattgtgcta
      301 gctcatggtt attctacagc tagtagtatg gcgaatgttg ccaaccgtat gctgaataag
      361 aacgtttttc aagcttatga tatgccttta gatattacat tagagaaaat tgaagcaaaa
      421 ttgattcaat tccttggaga ttatcgaaca gattcaggct tgattttatt ggtagatatg
      481 ggttcactaa accaactagg cggtttatta gtgaatcatc taaatggacc actgttaatc
      541 attgatcata tcaatacacc gttattattg gaagtcggcc aacaagtatt aaacaataaa
      601 agtatcaccg aaataggagc agaaattgaa acaagtctta ctttcaaaaa gcagttacta
      661 cttcccaaga aaaagaaaaa gaaagccatc attacttgtt gttatacagg gattggtagt
      721 gcgacccaga ttcaggagat tttaacgaaa tgcttgaaag atgcagttag agaattagta
      781 attattccgt atgattacaa aaagctgtta gaaaacaaga attttgagac tccttttcaa
      841 ttatacgatg tcatcatgat tgtaggtaca gaagatccca aaattaatca gattccatat
      901 attggattag atcgcctgat taatggggga agcattggcg aatttgttca ttacttggaa
      961 aaagagctga ctattgaagt gaacgattta caaaaagaat taattttcaa tttttcaatt
     1021 agtaaaattg tcgaaaattt aaccattttg gatccagata aggtgctatc ttttgtacaa
     1081 aaagcaatta aagaattatc gaagttatta aattttgatt tttctaataa tcagctgttt
     1141 ttgttgtaca ttcactgtag ttgtatgatt gaacgaattg tacgtaaaga aagtgtggat
     1201 gagcaagagg acattgaaga atatatgcaa gcaaatggag aaataatgga taaaattcat
     1261 agcgcttttt ttgagattga aagggaatac acaattgaaa taccagattt agagttgcga
     1321 ttattgagcg atattgttca aggataggag tgaattcgaa tgacaatggg agtaattttt
     1381 gatatggatg gggtactcat agatagcgag acattttatt ttgagagaag aatgaacttc
     1441 tttcaagaac tggagataga tccaggatca aaccagattg atgattatgt ggggaaaaca
     1501 gaagctgcaa tatgggaaac attagttcct gaggacaaat cattaagaga ggaactttat
     1561 ccgaaatatg tagattatcg aaaaacgcat ccaattgatt tcgctaaagc tttacgaaaa
     1621 gaagtgaaac cgttacttga cttcctcaag aagcgggata taccattggc actggcatct
     1681 tcttctccag ctgtcgagat agagagtatg ctcattcaat gtgaacttac tgaatatttt
     1741 tcctttgtta taagtggtga gtcattaaaa caaagcaaac cacatcccga aatatattta
     1801 aaagcgaaac agcaattaga ctgtgacttc tttattgcag tagaggattc aacgattggt
     1861 attaaatcag caaaggcagc aaatatctat acggcggcac taaaacaacc aatgcaaatc
     1921 gatcagagtg aagcagacat acaaattgaa agtctacaag aactggaaca aattgtttta
     1981 gaaaaaggtt agtgaattaa tggaaataag gctatatatc tgtgctagct attttgacta
     2041 accaaaaaaa cttgctataa ttcaggtgta gtaaaaggtg ctggtaacac cttttacgaa
     2101 aatgcttcgg cagtgataat gggattggct ttttaagttt tttattttgt tcgcaatccc
     2161 taaggtcatg tgaatgatcc tagggattgc tagaatattt tgcaacagaa ccaaaatacg
     2221 aatagttatc aagcagccat attgactgcg gtcaatttag gaggggatac tgatactact
     2281 gctgccgtaa ctggtgctct tgcgggtatt ctttacgggg gtgaagtgat ccctgaaaat
     2341 tggagaaaat cgcttcaaaa tgaccagctt cttaaaacga tcagtcagag attttgcgat
     2401 accgtgctat ctcaaagaat ttgaataatg gatatgatgc aaatcttgtt tgtactaacg
     2461 ttgtattgac aaaaaggatt ttatttagta caatgttagt acaaatgatg aacggaggta
     2521 gaatcatgtc tcaatcaacc attacattta ggattcccga cgatgaaaaa gaactggtat
     2581 tagaatatgc aaaagcacac aattcttcat tgaccgaact ttatcgaaat gctgtactgg
     2641 ataaaatcga agatgaaatc gatctacgga ctttacggag tgcaattgaa gtttccaaag
     2701 agaaaaaaga gagtggtatt tctcaagagg aaatggagaa actgctaaat gaattataaa
     2761 gtcatctaca tgccgcaagc ccagaagcaa ttaaaaaaaa tggacagaaa ccaagctaaa
     2821 ataattgtcg cctggatcag aaagaattta gaaaacacga ctgacccgcg tcagcacgga
     2881 aaaggtttga ctggaaataa gagtggggaa tggcgctatc gaattggaaa ctatcgaata
     2941 ttggctaata tttgtgattc tgaaattgaa atccagatat tctcaattgg acatagaagt
     3001 acaatatata aaagataacc gtttttggaa cgaagtagag tttcgaaagt caatgtataa
     3061 tcttttgaat tttgcataat accacaggta gaaactaccc gatactatcg gttggtttct
     3121 gctttttt
//
LOCUS       DACDXO010000127         3102 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E23605_2017
            SAMN12639989-rid9881463.denovo.135, whole genome shotgun sequence.
ACCESSION   DACDXO010000127 DACDXO010000000
VERSION     DACDXO010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639989
            Sequence Read Archive: SRR10023389
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3102)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3102)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741914.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:42:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,972
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 33 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 17 of 73
            Pseudo Genes (multiple problems)  :: 10 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3102
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639989-rid9881463.denovo.135"
                     /isolate="E23605_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..143)
                     /locus_tag="IWM35_002714"
     CDS             complement(<1..143)
                     /locus_tag="IWM35_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAP9920523.1"
                     /translation="MSNEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMRDVGDKKVQ
                     FTF"
     gene            complement(1484..2053)
                     /locus_tag="IWM35_002715"
     CDS             complement(1484..2053)
                     /locus_tag="IWM35_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAP9920524.1"
                     /translation="MTIKWIISIAYLVLTFAIPSLGVPSNIYFLFEHSDIFFLVLIII
                     LFHSTFIREFKEVNLKKLFKYSFFSFSVLFLINILNTSFSEGINPNEVNGSLLLFFLN
                     AATYGAFLEEGIFRFCMIDPQANKKQQYISILISSFLFSIVHGGGLSIFFIGIILSFV
                     YIQTKNIWYSIVAHGFYNTIGILIYLISI"
     gene            complement(2053..2508)
                     /locus_tag="IWM35_002716"
     CDS             complement(2053..2508)
                     /locus_tag="IWM35_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9920525.1"
                     /translation="MKKQLFKTFLLSTSILTFGSAITPSGAVFANENNISDIATYSDD
                     EYYYVLDEPVIQTRTWSKPQSWSIAKGSRTYLGIETHSRGTKLSVSANVFGMKIEIAR
                     NTSASGKFKKYRQNARITVTYRVYRKVDNKYIRTQTSTANTQYIDYIPA"
BASE COUNT         1041 a          537 c          496 g         1028 t
ORIGIN      
        1 tcgaatgtaa attgtacttt tttatcacct acatcacgca ttcttgatac gatagaaaag
       61 aacaaattca tttcgactgg tgtaaatttt cgcaacggaa ttgtatttaa ttcatgatga
      121 tactttacga tttcattaga catatttaaa atagctcctt tcttactcgc acaagagata
      181 cacgttatga aatacaaaat gcctatttat ctacatctta tattaacatc gtcgatgtac
      241 taattcaatg tacaaacaga caaaacgatg tagataaaca gacaaaacga tgtagataaa
      301 cagacaaaac gatgtagata aacagacaaa acgatgtaga taaaagcgtc attcccttgg
      361 gagagtaagg atatagccga cggtaaagag gtttaaaaag aggtttaaaa agagattata
      421 aaagaggggg acaaaatttc ccgcctaaaa aaccacttct tttttctcca aacagattcg
      481 cgcatttttc tcaactggga atcttactcc ctacagtcgc ttaaaaaggt gtgatcgctc
      541 tgctctcaca ttatactgcg cagtcgcttc cggctccttt gctaaaaata ttcataagga
      601 actctttgag aagctatgag ccttcgtttg agacgtttta agacagtctt agtataaatc
      661 ccttatcttg ttcttaaagc gccttacagg cggtcagggc aagccctaac aaccctaaag
      721 ttgtctgaat gccctctaaa attcgattta agcactctta aacctattct tatatattta
      781 tatacttaga cgtttcaaac gtcttaaaac gcaaattaga gcttgataaa gatatttgct
      841 cgtgtgccga cttttttaaa agtctaggca cacgccatga caaagctgaa ataaactgct
      901 tcagctttgt catggcggat tcgctcactt gaaaaagtgt tcatttcgct cgattattag
      961 catatatttt ttaacttttt ttgctagcca aattttgcat tagttcgctc ataaatccca
     1021 ccctttcagt acgtcctacg gacgtatcgt tcgtaatgtg aaagtacctt taaaggtact
     1081 ttctacaaaa ctcactaaag ggtttggcaa gctctatatt tgctagccaa atacctacgt
     1141 atttggtgca aataaccgct tgtcaaaggg gcgcaagctc ccccttgaat cccccctcaa
     1201 aacccacgct ccacaataca aaaaaggcta gcaaatgcta gccacttttt gtaaagtttc
     1261 gcttgtttca ccttcggtga aattgttgct ttgcaacgat caggtagagc tacatttttt
     1321 ttcgttgttc aaatattctt acgccattta ctattaaaat cgtaggtatt ccaaagtaaa
     1381 gaaaaaattg attagcaggt aatacaaaat ataaaataat accagcaata attaccaaac
     1441 ttatagtaag ttttgttaat ttcatgaagt atgccacctt atcttatata gatatcaaat
     1501 agattaatat gccgattgta ttataaaatc catgagcaac tatggaatac catatatttt
     1561 ttgtttgtat ataaacaaaa cttaaaataa taccaataaa gaaaatactt aggcctcccc
     1621 catgaacaat agaaaacaga aaagaagaaa ttagtattga aatatattgt tgttttttgt
     1681 ttgcttgagg atcaatcata caaaatcgaa aaattccttc ttctaaaaaa gctccataag
     1741 tagcagcatt taaaaagaat aaaaggagag aaccatttac ttcattagga tttatacctt
     1801 ctgaaaaaga tgtatttaga atattgatta ggaataaaac agagaatgaa aaaaaactat
     1861 atttaaagag tttttttaga tttacttctt tgaattcacg aatgaaggta gaatgaaata
     1921 gtataattat tagcactaaa aaaaatatat cactatgttc aaataaaaaa tatatatttg
     1981 acggtactcc taatgatggg atagcaaagg tcaaaaccaa gtaagcaata ctaattatcc
     2041 attttattgt cattaggcag gaatataatc aatatattgt gtatttgctg tagaagtttg
     2101 agtacgaata tatttattat ctaccttacg ataaactctg taggttacag ttattcttgc
     2161 attttgacga tattttttaa attttccact tgcagaagtg tttctggcta tttcgatttt
     2221 catcccaaat acatttgcac tgacagataa ctttgtacct cgactatgag tttctatacc
     2281 taaataagta cggcttcctt tagcaattga ccagctttga ggtttagacc aagtccttgt
     2341 ttgaataact ggttcgtcta acacatagta gtactcatca tcagaataag tggctatatc
     2401 agaaatgttg ttctcattag cgaataccgc tccgctagga gtaatagcag aaccaaaagt
     2461 taaaatagaa gtagataata aaaaggtttt aaataactgt tttttcatca aaatcacctc
     2521 gaatataaga tacttaagaa aacgctttct ttatataatt agaataatca ggaaatttaa
     2581 tgttgtcaag agggtttatt ttataaaagc tgttatatta aaggttagta gacataatgt
     2641 acactatgcc aagtagcccc tctaccaatg gatagagacg ctcactttgt gatttctaat
     2701 tggtctagct taaacacttc agccaatgct tgattttatg cacttttctt ttttcatttc
     2761 tttgtgcatt ttgaccggtc attttttaga gatttgttat ttttgtgagc atttgatatg
     2821 ctctttttat acctcaatct cttcttttct aatgttaatg aataggataa tctcattttt
     2881 agctgctcgt gagagcagtc tttttttgtt tcagggtacg tcctattctt tggatttccg
     2941 cgctattaaa taaccgatac ttggggattg gggtagcgcc ccaaaataca aaaagaagaa
     3001 gttgcataag cggatttttc ttttttttcg caagttggta ccaacttgca aatcttgctg
     3061 gaagactgat ccctaaaaag aaatcgagga atttttttag gg
//
LOCUS       DACDXO010000128         3013 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E23605_2017
            SAMN12639989-rid9881463.denovo.136, whole genome shotgun sequence.
ACCESSION   DACDXO010000128 DACDXO010000000
VERSION     DACDXO010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639989
            Sequence Read Archive: SRR10023389
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3013)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3013)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741914.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:42:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,972
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 33 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 17 of 73
            Pseudo Genes (multiple problems)  :: 10 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3013
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639989-rid9881463.denovo.136"
                     /isolate="E23605_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            177..359
                     /locus_tag="IWM35_002717"
     CDS             177..359
                     /locus_tag="IWM35_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HAP9920526.1"
                     /translation="MINLFLLVTASTLFFTGISLIISYLIYKKAENHTSKAIGLAVSS
                     FLTLLTLFVTLSFSFV"
     gene            462..746
                     /locus_tag="IWM35_002718"
     CDS             462..746
                     /locus_tag="IWM35_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9920527.1"
                     /translation="MLTATLQQPTHVAIAESYTINAGIVKQLIKDREFKKFNSRQAYN
                     RQLKKAILKHKDSFIGNLESVDVKTVSTAFSVVEVTIDGRLISLFAFGIN"
     gene            762..998
                     /locus_tag="IWM35_002719"
     CDS             762..998
                     /locus_tag="IWM35_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9920528.1"
                     /translation="MEGKTFAEIWTEFIEVPTRIDPITSEKVLDADFYNLKAGTSLEC
                     VWMYLEEHFKGVTLGHVLYHNGAEWDAIGNIHFS"
     gene            1020..1274
                     /locus_tag="IWM35_002720"
     CDS             1020..1274
                     /locus_tag="IWM35_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9920529.1"
                     /translation="MNYTDEEQIALEYVFTNEEGEEIEKVLIISSKDLEKALAYEPYE
                     STVTVIEFLDTYTSNDTWEIMNICNANNIQFAEKTNKSFY"
     gene            1356..1541
                     /locus_tag="IWM35_002721"
     CDS             1356..1541
                     /locus_tag="IWM35_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HAP9920530.1"
                     /translation="MPKTAREIVKILKSNGFKKTGQTGSHAKFYNSKTKRTVVVPIHN
                     GDVPLGTEANIWKQAGL"
     gene            1608..2003
                     /locus_tag="IWM35_002722"
     CDS             1608..2003
                     /locus_tag="IWM35_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin HicB"
                     /protein_id="HAP9920531.1"
                     /translation="MKTKNIFYPAIFEKEGEAYNIVFPDLPEISTFGNSLEEAYLNAK
                     DALGLALYSVPDAEFPKPSAVEELTLKANQVVVIIQLNIKLFRRSLNTKTIRKNVSVP
                     EWLVLLGKEKNINFSQLLQKALEEELLEK"
     gene            1993..2370
                     /locus_tag="IWM35_002723"
     CDS             1993..2370
                     /locus_tag="IWM35_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9920532.1"
                     /translation="MKSKKETEPMKATHLNYFSRTLINNLLKDTTGISVSTKHFANDL
                     IIFEDNNLRIEMFIEQATPNSIPEFCYTHTEKMKHAKSVKDVEALSESLTNALNLVKK
                     AKLLFEFQTDYHNELLAQSEILD"
     gene            2371..2766
                     /locus_tag="IWM35_002724"
     CDS             2371..2766
                     /locus_tag="IWM35_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9920533.1"
                     /translation="MKQEKRKMKQTQTETVNDTLADQLAGYYKLFEMGKAQRLIHYLW
                     KYKILFLFWESTLTFSVLFYKSDFYNQHLVLGSFLLIYTILMLNLYTYITLEEHLVIS
                     DAGIERNIKRVSFKITLNKPTDKKLSEAF"
BASE COUNT         1058 a          507 c          523 g          925 t
ORIGIN      
        1 tttatgttta tgtatatgta tatgaatata aacgtattag gtacgaacac ttttaaattc
       61 ttttctgggc tttgttttta gggtagggct aaaaaaaaca tcaccatctg atccaaaaca
      121 gctcagaaac gcattttaac gtatctgata ccaatattac agaaggagtg tatcgtatga
      181 ttaacctatt tcttttagtg acagctagta cattattctt tactggaata tcactgataa
      241 tttcctattt gatctataag aaagccgaaa atcacacaag caaagcaatc ggactagccg
      301 tttcatcctt tttaactttg ctgactttat ttgttacttt atcgttttct tttgtttaga
      361 acagctgatc cattagcacc tttttctctt tacattatac acactgttgt gtataatgta
      421 tttgtaaaca agctaattaa cttttaggaa ggagtgacaa gatgctaaca gctactttac
      481 agcaacctac tcacgttgct attgcagaaa gttatacaat taatgcaggg atcgttaaac
      541 agctgatcaa ggatcgagaa ttcaaaaagt ttaacagtcg gcaagcgtat aacagacagc
      601 taaagaaagc tattctaaag cacaaagatt ccttcatcgg gaacttggaa agtgtagatg
      661 ttaaaactgt ttcaacggca ttttcagttg ttgaagtgac tattgacggt cgcttgattt
      721 ctctatttgc ttttggaatt aattgatagg gagtgtgaac tttggaaggt aagacttttg
      781 cagaaatttg gactgaattt attgaggtac caactcgcat tgatccgata acttctgaaa
      841 aggtattgga tgcagacttc tacaatctca aagcaggtac tagccttgaa tgcgtttgga
      901 tgtatttaga agagcatttc aaaggcgtaa cgctcggtca cgtcttatac cacaatggtg
      961 ccgagtggga tgcaattgga aatatccatt tttcttaata taagaaagga gaacacaata
     1021 tgaattatac cgatgaagaa caaatcgctt tggaatacgt tttcacaaat gaagaagggg
     1081 aggaaattga aaaagtcttg atcatttcat caaaagactt ggagaaggct ttggcttatg
     1141 aaccatacga atcaaccgta actgtcattg aattcttaga tacctatact tctaatgata
     1201 cttgggaaat tatgaatatt tgcaacgcta ataacattca atttgctgaa aaaaccaata
     1261 aatcatttta ttagttttgc aataaaacat cctctttttc tattgattcc atacacaaca
     1321 atgtgtataa tagattttgt aaagggaggg tagctatgcc taagacagca agagaaattg
     1381 taaaaattct caaatctaac ggcttcaaaa agactggaca aactggcagt catgcaaaat
     1441 tttataattc caagactaaa agaacggtcg ttgttccgat ccacaatggt gatgtaccac
     1501 taggaacaga agctaatatc tggaagcaag cagggcttta acagccttgc cttcctattt
     1561 ggtattaggc atactatcct ctcttacact agaaaaggag cgaggaaatg aagacaaaaa
     1621 atatttttta tccggcaatc ttcgagaaag aaggagaagc ttataacatc gttttccctg
     1681 acttaccgga aatatctacc tttggaaata gtcttgaaga agcatattta aacgctaagg
     1741 atgctttagg attagcgcta tatagtgtac cagatgcaga atttcctaaa ccatcagcag
     1801 tggaagaatt aactttgaaa gccaaccaag tggtagtaat catccaacta aacattaaac
     1861 ttttccgaag aagcttaaat acaaaaacaa ttagaaaaaa tgttagtgtt ccggaatggt
     1921 tagttctttt agggaaggag aaaaacataa acttctctca actacttcaa aaagcactag
     1981 aagaagagtt gcttgaaaag taaaaaggag accgaaccta tgaaagctac acacttaaac
     2041 tatttctctc gaacgttaat caataatcta ttaaaagata caactggcat tagcgttagt
     2101 accaaacact ttgccaatga tctaattatt tttgaggata ataacttgcg aattgagatg
     2161 tttattgaac aggcaacccc taactcaata cctgaattct gctatacgca cactgaaaaa
     2221 atgaaacatg caaaatcagt taaagatgtt gaagcattaa gtgaatcact aacgaacgct
     2281 ttaaacctcg ttaagaaggc aaaattgtta tttgaatttc aaaccgacta ccacaatgag
     2341 ttgcttgctc aatctgaaat tttagattaa ttgaaacagg agaagagaaa gatgaaacaa
     2401 acacaaacag aaaccgttaa cgatacgctt gctgatcaat tagcaggtta ttataaatta
     2461 tttgagatgg gtaaagcaca gcgcttgatc cactatcttt ggaaatacaa aattttgttt
     2521 cttttttggg aatcaacact aacattctca gttctttttt ataagtcaga tttttataat
     2581 caacacttag ttttagggag ttttttgctt atctacacca ttttgatgct taatttatat
     2641 acctacatca cacttgaaga acacttagta attagcgatg cagggatcga gagaaacatc
     2701 aaaagggtat ccttcaagat cacgttaaac aagcctaccg ataaaaagct ctcagaagcg
     2761 ttctgagagc ttttgacatt ttcttgtata attttatcct aacacattac aaagcaccta
     2821 attcgagctt atatcaagca aatttgctat ttattactca atacatatct aaaaattact
     2881 tgatggaaaa ttagatacat ggtaagcatg aaaagaaaat caatttgagc agatgcagta
     2941 caatacatag caatttaaaa gctctcagaa ggcttctgag agcttttaat atattctttg
     3001 ataatttgtc ttt
//
LOCUS       DACDXO010000129         3007 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E23605_2017
            SAMN12639989-rid9881463.denovo.137, whole genome shotgun sequence.
ACCESSION   DACDXO010000129 DACDXO010000000
VERSION     DACDXO010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639989
            Sequence Read Archive: SRR10023389
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741914.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:42:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,972
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 33 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 17 of 73
            Pseudo Genes (multiple problems)  :: 10 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3007
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639989-rid9881463.denovo.137"
                     /isolate="E23605_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(276..692)
                     /locus_tag="IWM35_002725"
     CDS             complement(276..692)
                     /locus_tag="IWM35_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9920534.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     EAKLNPSNRNRPKNHQKETSQTVTSRNFDPTNFLKR"
     gene            complement(695..979)
                     /locus_tag="IWM35_002726"
     CDS             complement(695..979)
                     /locus_tag="IWM35_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9920535.1"
                     /translation="MDILVRNIDPKFVATIDKRCQELTNKTGEKWSRNKYLKVLIESD
                     FDRQLTTYKKDQFDLLLDKFIAIQTENTQILSEYVYTNNQIIEMLIGGMI"
     gene            complement(1679..1774)
                     /locus_tag="IWM35_002727"
     CDS             complement(1679..1774)
                     /locus_tag="IWM35_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HAP9920536.1"
                     /translation="MCFVFTNFICSLLVGLLSALFSYWLNNRNKK"
     gene            2427..>3007
                     /locus_tag="IWM35_002728"
     CDS             2427..>3007
                     /locus_tag="IWM35_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAP9920537.1"
                     /translation="MDEKTELTVKYQNELNLVPLKNFNAKQMDLFFALCARMKDRGLK
                     KVRFSFEELKELSDYKMTATKAFVGDLEKLYKDMLNLTYRTEDEDSIRYFVLFTGFEI
                     NKTKKFVEVSVNPDLEYIINGLTAEFSRFELSAFTSLRSTYAKTLFRLLMQYRSTGYY
                     VVRIEEFRSLMDIPEYYQMGNIDQKVLNPALREL"
BASE COUNT          957 a          478 c          565 g         1007 t
ORIGIN      
        1 aaaaaaatac ttcaatgatc ttaaagtcac aaaaataaaa gcaaaaaagg gcaataaaat
       61 cgccaaatta gaatttactt tttcaagcct aacgtcagat ttgccaaaga tttctctgcg
      121 taactggtta gaagaataaa taaaaaagaa ctcgcccccc ttacattaag caagtagaca
      181 agtcccttac aaccgactaa gattgctagt acattctagc atattgtatg gggcttctaa
      241 ataaaaaaca gataggaata actcctatct gttttttatc gttttagaaa atttgttggg
      301 tcgaaatttc tagaagtgac tgtttgactg gtttcttttt gatgattctt tggtcgattt
      361 cgatttgatg gatttagttt agcttcgatt ttttctttca cttcttgata tactttagaa
      421 tttgtgccaa taagaacgcc tgtatctttt agatataaat cagcaaattc tgcgatgatt
      481 tcagtcatga tggaaatttc ttttcccata gcatttagtt ttgcctgatt gctggtagtg
      541 gtcgtgttgg tatcgggttc ataactcata cataaaaagc gaatggcttc cgagtagtta
      601 ttgatatctg gatttttttc gatgatggaa tctaaaacgt caacatgact agggtacaaa
      661 gtaacatgtt tttggacaga agtttttctc atggttatat cattcctccg ataagcattt
      721 caatgatttg attatttgtg tagacatact cgcttaatat ttgagtattt tctgtttgga
      781 tagcaataaa tttatcgagc aataaatcga attggtcttt tttgtaagtc gtaagttgac
      841 ggtcaaaatc actttcgatt aaaactttta gatatttatt tcgactccat ttttctcctg
      901 ttttattcgt gagttcttgg caacgtttgt ctattgttgc aacaaacttt ggatcaatat
      961 tccgaactaa aatgtccata aaaaatctcc ttttgcaatt atgtataatt tatgtatacc
     1021 atacaaatac tatatttgta tagctgtttt ttgccaaaat gctatacata agctacccac
     1081 gatggcgaaa aagtgatatc aaattttttt ctgagaaacg tgataggcac agcctatcta
     1141 cccacttttt ttagaaagtg ggtacacgtt tccttatgct ctttaaaatt cgggggggag
     1201 tgctgcacgt gtaatcgata accaaattta acagacgaaa aattgttgga atataaaaat
     1261 atgacacccg aaagaaaacg atcgttggaa aaattaatgc aactagtgaa cgaagacggc
     1321 ggaatttcag acgcagcttt tgacgcatgg agcgaatttc atggagaata taattaagtg
     1381 ggtttaaatg gctctaaaat cgcttctaag agttttttag cttggaccaa tgaaatacac
     1441 caaaaactaa ataaaacgtc ttaaatcgaa aaatacgagc tctcagagcg actttatttg
     1501 taaatgttgc atataagaaa taattacgga ttttagactt ttgtaggata tttttgaaat
     1561 aaatatttat ttctgttata atttgattgc aaataagtga acacgaaaca cacgaaatcc
     1621 ccagagaggt cggattctct ggggcttttt ttgtgggctg agtcgcctta attaagtttt
     1681 acttcttgtt tcggttattc aaccagtaac tgaacaaagc gcttaaaagc ccaacaagaa
     1741 gggagcaaat aaagttagtg aacacgaaac acacctcctt ccagtcgcaa gactgtcgga
     1801 taggcgacaa tcaattatac aataaaaaag caaaaataag cctaagtttt ttattgcctt
     1861 ggggttgtta gggcttgccc tgaccgcctg taaggcgctt tagaacgaag gacatagttt
     1921 ttatcgaatc ttgtcttaga acggcttaga cgagctctca ggacgccaca aaggagtccc
     1981 taatgaatta tttttggcga aggagcctaa aggcgactgc tgcaagtaaa atgtgagagc
     2041 gtagcgatca ctccttctta cagcgaccat agggagctag attggcagtt taatatcatg
     2101 cgcgaacctg tttattttaa aaagtgggtg attttttggc gggaaatttt gctcgcgcgc
     2161 gcgcgtttat tttatttatt ttttaaagat tttttagaac cctttttagg gggcagttga
     2221 gccttactct cccaacggat acagaagggt actaacaact ttttatctga gtactaacaa
     2281 ctttttatct gagtactaac aactttttat ctgagtacta acaacttttt atctgagtac
     2341 taatatcctt ttgtataata acaaccaacc tatttacgag ttggttgtta aaggttatta
     2401 tttatttgta aggggggaag aaaggaatgg atgaaaaaac ggaattaaca gtcaaatatc
     2461 aaaatgaatt gaatttagta ccgctgaaaa atttcaatgc taagcagatg gatttatttt
     2521 ttgcattatg cgctcgtatg aaagatcgtg gtttgaaaaa agttagattt tctttcgagg
     2581 aattaaaaga gttaagtgat tataagatga ctgctacaaa agcctttgtc ggtgatttag
     2641 aaaagctcta taaagatatg cttaatctta cttaccgtac tgaagatgag gattctattc
     2701 gctactttgt tttgtttact ggttttgaaa tcaataaaac aaaaaagttt gttgaagtca
     2761 gtgttaatcc ggacctagaa tatattatta atggtttaac agctgaattt agtagatttg
     2821 aattatctgc tttcacgtcg ttgcgttcta cgtatgcaaa aactctattc cgactgctta
     2881 tgcagtaccg ttctactggt tattatgtag ttcgtattga agagtttagg agcttaatgg
     2941 atatcccaga atactatcaa atgggtaaca ttgatcaaaa agtattaaat cctgcattac
     3001 gagaact
//
LOCUS       DACDXO010000130         2947 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E23605_2017
            SAMN12639989-rid9881463.denovo.138, whole genome shotgun sequence.
ACCESSION   DACDXO010000130 DACDXO010000000
VERSION     DACDXO010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639989
            Sequence Read Archive: SRR10023389
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741914.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:42:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,972
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 33 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 17 of 73
            Pseudo Genes (multiple problems)  :: 10 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2947
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639989-rid9881463.denovo.138"
                     /isolate="E23605_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(449..865)
                     /locus_tag="IWM35_002729"
     CDS             complement(449..865)
                     /locus_tag="IWM35_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9920538.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIERNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMSEIIAEFADLYLKDTGILIGTNSKVYQETKEKI
                     EKQLNPSKRNQPKNRQKETNQTFTSKNFDPTNFLKR"
     gene            complement(867..1148)
                     /locus_tag="IWM35_002730"
     CDS             complement(867..1148)
                     /locus_tag="IWM35_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9920539.1"
                     /translation="MDILVRKIDSKVIAKIDKRCQELTNKTGKKWSRNRYLKVLIEND
                     FDHALMHYKKDQFDLLLEKFIAIQEKNTETLNEYVRTNNQLIETLIGGR"
     gene            1948..2922
                     /locus_tag="IWM35_002731"
     CDS             1948..2922
                     /locus_tag="IWM35_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HAP9920540.1"
                     /translation="MSKRGTIINEWEKLNRISNRRLNAQTMCTPLKMQWSIDRITIVG
                     TLRENIYYHTPHDVLILDFEQLMRLNEGNGYLKAVGNNGWQLLDQHEENIAYIEILKF
                     QEGKGRIDFNPNKISQFLAGSMKNFIHDLFLEPHFSRADIACDIIDVPDDFITQYRVV
                     DPVSFKPIYGRSGKLETAYWGSRASERQIRMYNKKLEQERKRKVVPKEIDTWWRLELQ
                     LRRGKATDWHAMVHESLDSFASPHYLPEDVKVTDRMMITALITEHSYWGQIHRNSKYK
                     YRELLKQESQNDELTNHLRETFSESADDLKRELDTWLQGLDVTEVGAE"
BASE COUNT          913 a          438 c          603 g          993 t
ORIGIN      
        1 aaaaaaactt gctgatgaat tgggtgtatc gaaaaatacg gtgcgcaatc aactggtcaa
       61 ttcgggattt gaccttgctc agtataaaga aaaaaatgca gtggtcaatt gaccgaaatt
      121 tatgtctttg ggttttgggg ttttaaaacg aaaaaaaggg ggcgattttc gccccctttg
      181 ctctatgtga aaaatttgtt atacttattt tatccttaaa acataccaat ctttttctta
      241 tttagctgct cgtacgagca gcttttttta cgattatgaa ttcaaatcat gtatttataa
      301 tttttttgtt tttatctttt tttctgttat ttcttctgaa attcgttttt ttcattcaat
      361 tggttactat cttattgtaa atgttgctat taagatggtg atcctaccca aaaaataaac
      421 caggaattta aatctcctgg tttatttttt atctttttag aaaatttgtt gggtcgaaat
      481 ttttagaagt aaatgtttga ttggtttctt tttgacgatt ttttggttga tttcgtttgg
      541 agggattaag ttgtttttca attttttctt tcgtttcttg atacacttta gaatttgtgc
      601 caataagaat acctgtatct tttagatata aatcagcaaa ttctgcaatg atttcggaca
      661 tgatggaaat ttcttttccc atagcattta gtttggcttg gttgctggta gtcgtagtat
      721 tggtatcagg ttcataactc atacataaaa aacgaatggc ttccgagtaa ttgttgatat
      781 ctggatttct ttcgataatg gaatctaaaa cgtccacgtg actgggataa agggtaacgt
      841 gtttttgaac agaagttttt ctcatgttat cttcctccga taagtgtttc aatgagttgg
      901 ttgtttgtac gaacatattc attcagcgtt tcagtatttt tttcttgaat agcgataaat
      961 ttttcgagta ataaatcgaa ttgatctttc ttgtaatgca taagtgcgtg gtcaaaatcg
     1021 ttttcgatta ggacttttaa gtagcgattt cgactccatt ttttgccagt tttatttgtg
     1081 agttcttggc aacgtttgtc tatttttgca ataacctttg aatcgatctt ccgaactaaa
     1141 atgtccatag aaaaactcct ttcgcatttg tgtataattt atgtatatca tacgaatata
     1201 gtatttgtat agctgttttt tgctaaaata ctatacataa gctagccacg atggcgaaaa
     1261 agtgatatca aatttttttc tgagaaacgt gataggcaca gcctatctac ccactttttt
     1321 tagaaagtgg gtacacgttt ccttatgctc tttataaaat ggtgatgtgc gacttttttc
     1381 tgttcgtaaa aataaaaaaa gtttacgaaa atgaaactaa actgaacgat tatgacgggc
     1441 atgtaatcaa gatgaaatga ttttataagc gcgtattttg cgttctaaga cgttttagcg
     1501 ttcatttcgt ttagttatcg gcacaatcgt taaaacacgt aagagaagcg agaatggagc
     1561 ctgtaaggcg actggtagcg aaacggcgtg ttagattgtg caagccgaga actgaatgaa
     1621 atgaagagcg gaagcgtgaa ataatttcgg caggaggagg ctcaaggggt tcggggaatg
     1681 aaattccctg aggtcttttt atttttttaa gaggagtgct ataatcaaag aaaacgagaa
     1741 aaaagagcaa aataaaagcc gaacggtgtt ggaagcaccc ttcggctaat tacggaaact
     1801 actcacctag ttccgtaact caaattcatt atacgaatat ttttaggttt cttcaagcta
     1861 gagatttttg gatatggatt tttagagtta cgtgaactct ggaaattctt tccaaaaatc
     1921 tcttttttct ttgaggagga acaaaatttg agtaaacgtg gcacgatcat aaatgagtgg
     1981 gaaaaactta accgcatatc taacaggcgg ttaaatgcac aaactatgtg caccccctta
     2041 aaaatgcaat ggtcaattga ccgaattacc attgttggta cattaaggga aaatatttat
     2101 tatcacactc cccatgatgt gttgatttta gattttgaac agcttatgcg tctaaacgaa
     2161 ggtaacggat atttaaaagc ggttggaaat aatggctggc aattactaga ccaacatgag
     2221 gaaaatatag cttatattga gattcttaag tttcaagagg gaaaagggcg gattgacttt
     2281 aaccccaata aaattagcca atttctggcg ggctcgatga agaactttat ccatgattta
     2341 tttttagaac cacatttctc acgtgcagat attgcttgtg acattattga cgtgccagat
     2401 gatttcatta cccaatatcg ggtggttgat cctgtttctt tcaaaccaat ttacggtcgg
     2461 tctgggaaac tggaaacagc atattgggga agtcgtgcta gtgaacgaca gattcggatg
     2521 tataacaaaa aattggaaca agaaagaaaa cggaaagttg ttccgaaaga aatcgatact
     2581 tggtggcgac tagaattgca attgcgaagg gggaaagcaa ctgattggca tgcaatggtt
     2641 catgaaagtt tagatagctt tgcaagtcct cactatttgc cagaagatgt gaaagtaact
     2701 gacagaatga tgattactgc tcttataacg gaacatagtt actgggggca aattcataga
     2761 aattcaaaat ataaatatcg tgaattgtta aagcaagaaa gtcaaaatga tgagttgacg
     2821 aatcatttgc gagaaacatt ttcggagtcg gcagatgatt tgaaacgaga attagataca
     2881 tggcttcaag gacttgatgt gacggaagta ggtgctgaat gagtgattcg atagaaaaaa
     2941 tgaccct
//
LOCUS       DACDXO010000131         2944 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E23605_2017
            SAMN12639989-rid9881463.denovo.139, whole genome shotgun sequence.
ACCESSION   DACDXO010000131 DACDXO010000000
VERSION     DACDXO010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639989
            Sequence Read Archive: SRR10023389
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2944)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2944)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741914.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:42:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,972
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 33 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 17 of 73
            Pseudo Genes (multiple problems)  :: 10 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2944
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639989-rid9881463.denovo.139"
                     /isolate="E23605_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..132)
                     /locus_tag="IWM35_002732"
     CDS             complement(<1..132)
                     /locus_tag="IWM35_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAP9920541.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     "
     gene            542..1687
                     /locus_tag="IWM35_002733"
     CDS             542..1687
                     /locus_tag="IWM35_002733"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HAP9920542.1"
                     /translation="MHIQTKLIHGGISEDPTTGAVSVPIYQTSTYRQDGVGQPKQYEY
                     SRSGNPTRFALEELIADLEGGVRGFAFSSGLSGIHAVFSLFQAGDHILLGDDVYGGTF
                     RLFDKVLTKNGLEYTIIDTSNLDKIEQSIKPNTKALYLETPSNPLLKITDLEKSATLA
                     HQHGLIVIADNTFATPYFQRPLDLGSDIVVHSGTKYLGGHSDVVAGLVTSNHKDLADQ
                     IGFYQNAIGAVLGPQDSWLLQRGIKTLSVRMEEHQKNAFVVADFLFSHPAVEKVYYPG
                     LPDHELHGVAKQQMSGFSGMISFTLKNEESAIPFVESLQLFTLAESLGGVESLVEIPS
                     VMTHASIPKEKREEAGIKDGLIRLSVGIEYGQDLINDLAQAFDRIKN"
     gene            1858..2316
                     /locus_tag="IWM35_002734"
     CDS             1858..2316
                     /locus_tag="IWM35_002734"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HAP9920543.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            2475..>2944
                     /locus_tag="IWM35_002735"
     CDS             2475..>2944
                     /locus_tag="IWM35_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HAP9920544.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
BASE COUNT          997 a          547 c          554 g          846 t
ORIGIN      
        1 tcgtatgaat gaagcacctt cgatgtcatt ggcaacagag atttcagata agatagacaa
       61 ttgattgtcg tctgttaaaa aacaaacatc tagtttttca gagctaacgt caataccgac
      121 aaataatttc atggaagaag gacctccttt cgatataaat tttggaaaat gcttcgatac
      181 tgtgggattt cccagaaact cactgtatag ccacaacctc gcctaataga atacggtgat
      241 aaaagagcta gaagctgcct tccaaactac tatttgaaag tctagtccat tatcgaaaca
      301 gctaatgagt tggtagtgat aacagtggtt cgggtaacag actaaacagg gtagacaaac
      361 gctacaggag gaaatagcaa attcccgttg gatcctttcc atacatccat tgtactctga
      421 gaaatcacac aatatcgaaa caaattccgt taggaatgtg ttattcaata aattttataa
      481 tctagaaaca ttttgaataa aaactattag acaaatgata ttttacgagg aggaaagaaa
      541 catgcacatt caaacaaaat tgatccacgg aggcatcagt gaagatccca caacaggagc
      601 tgttagtgta cctatttatc aaacatccac ttatcggcaa gatggcgtcg gccagcctaa
      661 acaatatgaa tattcaagat ctggtaatcc tactcgtttt gcattggaag aactgattgc
      721 tgatttggaa ggcggtgttc gaggattcgc tttttcttct ggtctttctg ggattcatgc
      781 cgttttttct ctttttcaag ctggcgatca tatcttatta ggagatgatg tctatggagg
      841 aacattccgt ctatttgaca aagttcttac taaaaatggt ctcgagtaca cgatcataga
      901 tacgagtaat cttgataaaa tagaacaatc catcaaaccc aatacaaaag cactttatct
      961 tgaaacacct agcaatccgc ttctaaagat tactgattta gaaaaaagtg ccactctggc
     1021 tcatcaacat ggattgatag ttattgctga caatacattt gctactcctt attttcaaag
     1081 acctttagat ctcggttctg atatcgttgt ccatagcggt accaaatatc taggcggaca
     1141 cagcgatgta gtagctggtt tagtgacttc taaccataaa gatctagccg atcagatcgg
     1201 attttaccaa aatgctatcg gagccgtact tggtccccaa gatagctggt tacttcagcg
     1261 cggaatcaag acactaagtg ttcgtatgga ggaacatcaa aaaaatgctt ttgtggtagc
     1321 tgattttcta ttttctcatc cagcagtaga aaaggtctac tatccaggat tacccgatca
     1381 tgaactccat ggagttgcta agcagcagat gagcggcttt agcggaatga tttcttttac
     1441 tttaaaaaat gaagaatctg ctattccatt tgttgaatca ctccaactat ttactttagc
     1501 agaaagttta ggtggcgtag aaagtttagt ggaaattcct tctgtcatga ctcatgcgtc
     1561 tattccaaaa gaaaaacgag aagaggctgg cataaaggac ggcttgatcc gtctttctgt
     1621 aggaattgag tatggtcagg atctcataaa cgatttagct caggcatttg acagaatcaa
     1681 gaactagaag tcccaataaa cgacttagca gaaaaatcta aaaaatattt cagattgcta
     1741 caccttggaa acgataagtg ttagaatcaa gtagaaagac gtttttgtaa atttcactaa
     1801 ataattcttt agtgaaaagc atcaaaacga atgaacaaac agataggagt cttacaaatg
     1861 agaatgaatg tcgaaagttt taatcttgac catacaaaag tcaaagcacc ttatgtacgt
     1921 gtagctgatc gaaaaacggg tgcgcatggc gatgtcatca ttaaatacga cgtccgcttt
     1981 aaacaaccaa ataaagaaca catggacatg ccaagtctac attcacttga gcacttgact
     2041 gccgaactaa tccgcaatca cgcagattac atcgtagact ggtctccaat gggatgccaa
     2101 acaggctttt acttgactgt attgaatcat gaaagctacg aagacatttt agatgttctt
     2161 gaagcaacaa tgaaagatgt tcttgaagct actgaagtac ctgcaagcaa tgaaacacaa
     2221 tgtggctggg ctgccagcca tacactagaa ggtgcacaag ctttggctcg tgaattttta
     2281 gcaaaacgcg atgaatggtc tgaagtagaa gcatagtttc taaaagaacg taaagatgag
     2341 aagcaatata gctatcttat cttcgtattt tattaaatat ttattgtctg aaaagagttc
     2401 taaacgggaa aacaaatagc tagttttcag tttagcgctc tttttattat ataaaataga
     2461 aaggagaaaa ctttatgtct atttataatt ttgaaactac tttagaagac ggcactgtct
     2521 attcattgaa aaaatatgca ggcaaaccga taatcattgt taacactgca acaaaatgcg
     2581 gtctagcacc tcaatttaaa caattagaag aactttacca agcttatcag acacaaggtc
     2641 tggttatctt aggctttcct tccaatcaat ttaaacaaga gctagattca gcgactgaag
     2701 cagcagaagc ttgtaaaacc acttatggcg ttaccttccc tatgcatcaa ttgattgacg
     2761 taaatggaga aaatacagat cctcttttta cttatcttaa aaatgaagct cctggtactg
     2821 tcactaaagc aataaaatgg aattttacta aatttttgat tgatagaaat ggcaaagtaa
     2881 tcgaacgatt tgcaccacaa acttctccat tgaaaatgac cgctgcaatt gaatccgttc
     2941 ttta
//
LOCUS       DACDXO010000132         2942 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E23605_2017
            SAMN12639989-rid9881463.denovo.140, whole genome shotgun sequence.
ACCESSION   DACDXO010000132 DACDXO010000000
VERSION     DACDXO010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639989
            Sequence Read Archive: SRR10023389
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2942)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2942)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741914.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:42:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,972
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 33 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 17 of 73
            Pseudo Genes (multiple problems)  :: 10 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2942
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639989-rid9881463.denovo.140"
                     /isolate="E23605_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..1344
                     /locus_tag="IWM35_002736"
     CDS             <1..1344
                     /locus_tag="IWM35_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP9920545.1"
                     /translation="LPALPEGTTVTDVTPGGTIDTNTPGNYEGVIEVTYPDGTKDTVK
                     VPVEVTDNRSDADKYEPTVEGEKVEIGGTVDLTDNVTNLPALPEGTTVTDVTPGGTID
                     TNTPGNYEGVIEVTYPDGTKDTVKVPVEVTDNRSNADKYNPEGQKVTTDLNKEPDASE
                     GIKNKEDLPKGTTYAWKEKVDVSTAGNKKGTIVVTYPDGSSDEVEVDVTVTDTRSDAD
                     KYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYTWKEKVDVSTAGNKKGTVVVTYP
                     DGSKEEVEVTISVVDKKAPNKPQVDPITEGDQIVTGKTEPNAEVTVTLPDGSQHHGTA
                     DKNGNFTVKVPKLEAGTKVIVTATDESGNTSEPTNVVVSSNKKDSGKNGSKGSKTDNQ
                     GSNSNQDKNRGKSQSSKVFPKTGESDSNIFTISGGLILLGTLGLLGYKNRKKENE"
     gene            1478..2821
                     /locus_tag="IWM35_002737"
     CDS             1478..2821
                     /locus_tag="IWM35_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAP9920546.1"
                     /translation="MEKIVIIGSNHSGLAAARFLNLSDSNYEVILIDKKSEISYLECG
                     TSLFIKGEVSNLEDLFYQNRMTLNSEIKKLFLETEVDKIDIQNKKISCVKKDGTRFSQ
                     EYDKVILATGSKQIMLDIPGNNLANIFSIKNFPNAKEIFKALDDSTIKKVGIIGAGYI
                     GVEIAEAIRKRGKEVYLFDVADRVLSTYYDRSFSDKVEEILSKNGIHLCLSESPKKYL
                     GRKKIEKIVTTSGTQYSMDMVVQAIGFLSNSPIGEKELLRDVSGAYLTNEYQQTNIKD
                     IYAIGDCATTFFTSINRSSVDFSISNALRTAYIATQHINNQDFTPSGSQFTNGFSIFN
                     YNFYAAGLNLKTARLLSESIDYIEIEDFIRPAFLRKNSRVKLRISFEKNTKRIVGVQL
                     CSQEDLSGLLSMFSLAIEEQITLDKLKMLDYLFHPSFSQPYNFILKAVTGLNIET"
BASE COUNT         1168 a          396 c          561 g          817 t
ORIGIN      
        1 ttgccagcgt taccagaagg aacaacagta acagatgtta ctcccggcgg tacaattgat
       61 accaacacac caggtaatta cgaaggtgtc attgaagtaa cgtatccaga cggtacgaaa
      121 gatacggtga aagttccagt agaagtgaca gacaatcgtt ctgacgccga taaatatgaa
      181 ccaacagtag aaggcgaaaa agtagaaatt ggaggcacag tagatttaac agataatgtt
      241 actaacttgc cagcgttacc agaaggaaca acagtaacag atgttactcc cggcggtaca
      301 attgatacca acacaccagg caattatgaa ggtgtcattg aagtaacgta tccagacggt
      361 acgaaagata cggtgaaagt tccagtagaa gtgacagaca atcgttctaa tgcagataaa
      421 tacaatcctg aaggacaaaa agtgactaca gacttaaata aagagccaga tgcatctgag
      481 ggaattaaga acaaagaaga tttaccaaaa ggaactactt atgcttggaa agagaaagta
      541 gatgttagca cagctggaaa taaaaaaggt actattgtag tgacatatcc agatggatca
      601 tctgatgaag ttgaagtaga cgttacagtg acagatactc gttccgatgc agataaatat
      661 aatcctgaag gacaaaaagt gactacagac ttaaataaag agccagatgc atctgaggga
      721 attaagaaca aagaagatct accaaaagga actacttata cttggaaaga aaaagtagat
      781 gttagtacag ctggaaacaa aaaaggtact gtagtagtaa catatccaga tggatctaaa
      841 gaagaagtag aagttactat ttctgtagta gataaaaaag caccgaataa acctcaagtt
      901 gatcctatta cagagggtga ccaaattgtt actggtaaaa ctgaaccaaa tgcagaggtg
      961 acagttacat taccagacgg aagtcaacac catgggacag ctgataaaaa tggtaatttt
     1021 acagttaaag ttcctaaatt agaggcaggg acaaaagtga tagtaactgc aactgatgaa
     1081 tctggtaata ctagtgaacc tactaatgta gttgtttctt ccaacaaaaa agacagtgga
     1141 aaaaacggta gtaaaggtag taagactgac aatcaaggta gcaacagcaa tcaagacaaa
     1201 aatagaggca aaagccagtc ctcaaaagtt tttcctaaga cgggagagag tgacagtaat
     1261 atctttacta ttagcggtgg tttaatactg ttaggaacgt taggattatt agggtataaa
     1321 aatcgaaaaa aagagaatga atagaaaatc aatttcttaa gtcttctctg agaagaggaa
     1381 gaatcttttt cttcctcttt ttgctttttt atgatgggaa ataagtgtta gttaaagatg
     1441 aatatacttt ttatagaaag gaaaagaatg taattaagtg gaaaaaatag taattattgg
     1501 ttcaaaccat tcagggctag ccgcagctcg atttctaaac ttaagcgatt caaattatga
     1561 agttattcta atcgataaaa aatcagaaat tagttacttg gaatgtggga catcgttatt
     1621 tataaaagga gaagtaagta atttagaaga tcttttttat caaaatagaa tgacactaaa
     1681 ttctgagata aaaaagttat ttctagagac agaagttgac aaaattgata ttcaaaataa
     1741 aaagattagt tgtgtaaaaa aagatggtac aagattctct caagaatatg acaaagttat
     1801 attagctaca ggttcaaagc aaattatgct agatataccg gggaataatt tagctaatat
     1861 tttttcgata aagaattttc caaatgcaaa agagattttt aaagctttgg atgatagtac
     1921 tattaaaaaa gtaggaatta ttggagcagg atatattgga gttgaaattg ctgaagccat
     1981 aagaaaaaga gggaaagaag tatatctttt cgatgttgca gatagagtgt tgtcgactta
     2041 ttacgaccgt agtttttcag ataaagttga agaaatacta tcaaaaaatg gaatacatct
     2101 ttgtctatca gaaagtccta aaaaatattt aggaagaaaa aagattgaaa aaatagtcac
     2161 tacaagtggt acccaatact ctatggatat ggtcgtacaa gctataggat ttctatctaa
     2221 tagtccaatt ggggaaaagg aactcttgag agatgttagt ggagcatatc taactaatga
     2281 ataccagcaa acaaatataa aagatatata tgctattggg gattgtgcta caactttttt
     2341 tacgtccatt aatagaagta gtgttgattt ttctatttct aatgctctaa gaacagctta
     2401 tattgcaact caacatatta acaatcaaga ttttacacct tctggttccc aatttacaaa
     2461 tggcttttct atatttaatt acaattttta cgcagcaggt ttgaatttaa aaacagcacg
     2521 attattaagt gagtctatag attatataga aatagaagat ttcattagac ctgctttttt
     2581 gagaaaaaat agtcgtgtaa aattaagaat tagctttgaa aaaaatacaa agagaatagt
     2641 aggtgtacaa ctttgtagtc aagaagattt atctggacta ttatcaatgt tttctcttgc
     2701 tatagaagaa caaattacat tagacaaatt gaaaatgtta gattatttat ttcatcctag
     2761 ttttagccaa ccctataatt ttattttaaa ggcagtaaca ggattaaaca ttgaaacata
     2821 atcgttacta aatgaaatca agaaatatac tgtattactc aattcattga aaatatttat
     2881 cataattttt aatagaaaac actattaaag agaaaaaaga gaggtaaatc aaatgaaaaa
     2941 at
//
LOCUS       DACDXO010000133         2886 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E23605_2017
            SAMN12639989-rid9881463.denovo.141, whole genome shotgun sequence.
ACCESSION   DACDXO010000133 DACDXO010000000
VERSION     DACDXO010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639989
            Sequence Read Archive: SRR10023389
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741914.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:42:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,972
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 33 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 17 of 73
            Pseudo Genes (multiple problems)  :: 10 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2886
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639989-rid9881463.denovo.141"
                     /isolate="E23605_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            798..1064
                     /locus_tag="IWM35_002738"
     CDS             798..1064
                     /locus_tag="IWM35_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9920547.1"
                     /translation="MAKITKNEQENLVENYTKKPAYSNSRLDAHFVCTSNPKLSFDAM
                     TIVGNLNKNSAKKLSDFMSLDPQIRLWDILQTKFKAKALQDPLC"
     gene            1122..1634
                     /locus_tag="IWM35_002739"
     CDS             1122..1634
                     /locus_tag="IWM35_002739"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003321317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP9920548.1"
                     /translation="MTNKNNERYSTELKATAIKKMMPPENRGIRQLSQTLGIPEATLY
                     SWRKKARIQGNTTPGTKKNVEKWSSEDKFLIVMESFSMNQVELAEYCRKKGLFKEQIE
                     AWKKTCLSANEQEENRTRELATELKEEKKQARQLEKELRHKEKALAEAAALLLLRKKA
                     HAIWGDQEEE"
     gene            1631..2668
                     /locus_tag="IWM35_002740"
     CDS             1631..2668
                     /locus_tag="IWM35_002740"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAP9920549.1"
                     /translation="MISSSDRKQAVELIQEANRNGARLTYACNELNISVRTYERWTRE
                     GTIAHDQRPLAKRPVPKNKLTDQEREKIIETVSKKEFMNLPPSQIVPKLADCSIYIAS
                     ESSFYRVLREKNMQHHRGRSQVSQKRIPPSHLATKPNEVWTWDITWLKGPIKGLFYRL
                     YLIIDLFSRKMVGWEIWESEEARHAEKLIKQTVLKERIQGQPLVLHSDNGSPMKAATF
                     QVLLEKLGIQSSYSRPRVSNDNPYSEAAFRTLKYRPEYPNNGFKTIDDARKWTKEFVD
                     WYNDSHLHSGIQFVSPAACHEGRHLAILEKRKQVYEDAKKRHPERWSKGVRNWTPHQS
                     VALNPLKETES"
BASE COUNT         1041 a          513 c          574 g          758 t
ORIGIN      
        1 ttgatgacca cgaaagtgca tggcgatatt tgatgttgat actttatgca gtaccctatg
       61 tattcttcgc tatcattctg tggctcatag cattgttttt tagagtattt aatgaccttt
      121 aggtagattt atatataaaa agggctaaat acgcaaaata cccccttaaa atgcaaaata
      181 aatgcaaata agggggtaac tccttatgaa aaaacaacta atagtattat aacacgtttt
      241 tatgccgaga aaacttattg gttggaatgg gctatgtgtt agctaacttg ttagcgagtt
      301 ggttggactt gaattgggat taatcccaag aaagtaccaa ctcaacaaca cataaagccc
      361 tgtaggttcc gaccaataag gaaattggaa taaagcaacg aaaggagttg agtaaaatga
      421 gtgagaaaac gaagaaaatc attcgagtat cattagcttt ttcacaagta gtagtgcaat
      481 cattgatagt ttattatact taccagcaat ataaattatc taaagagaat taagttttga
      541 ttatgtaata agttttggta ctaaaaaaga cttgatctaa ttagaccaag tcttttgtta
      601 gtgttatatt aataacaaaa taaaaataag aagtcgctca ctccccgacc aaagtttgtg
      661 agcaaaataa acagtcgatg tttgtttctt aattgtatat cttgatatta aacgatttaa
      721 gattattctt caagatatat attcgcaaat gtaattggaa aagcgacttc ttaaacaaaa
      781 ttaaggagtc gctttttatg gcaaaaataa cgaaaaatga gcaagaaaat ttggtggaaa
      841 attacacaaa aaaaccggcg tactctaata gccggttaga cgcacatttc gtgtgcactt
      901 ctaatccgaa attgagcttt gacgctatga cgatagtcgg aaaccttaat aaaaacagcg
      961 ctaaaaagct atccgatttt atgagtttag atccacaaat tagactttgg gatatacttc
     1021 aaacaaaatt taaagctaaa gcactacaag accccctatg ttaggctagt tgtcgtatag
     1081 gataataagt tataataaag actatacgga ggatgattac catgacaaat aaaaacaatg
     1141 aacgttactc taccgaactg aaagcgacag ccatcaagaa aatgatgcca cccgaaaata
     1201 gggggattcg ccaattaagt caaactttgg gcattccaga agcgactctt tattcttggc
     1261 gtaaaaaagc tagaattcaa gggaatacta cacccggaac gaaaaaaaat gtcgaaaaat
     1321 ggtcaagtga agataaattt ttaattgtga tggaatcatt ctcaatgaac caagtagaac
     1381 ttgcggaata ttgccgaaag aagggcttat ttaaagaaca aattgaggct tggaagaaaa
     1441 cgtgcttatc tgcgaatgaa caggaagaaa atcgaacgag agaacttgca actgaactaa
     1501 aagaagagaa aaaacaagcg cgtcaattag agaaagaatt acgtcataag gaaaaagcat
     1561 tagcggaagc cgctgctctc ttattactaa gaaaaaaagc acacgcgatt tggggggacc
     1621 aagaggaaga atgatttcat cttcagatcg caaacaagct gttgaactta ttcaagaagc
     1681 caatcgcaat ggggcccgtc tcacctatgc ctgtaatgag ctgaatatta gcgttcgaac
     1741 ctacgaacga tggacgcgtg aaggtacgat agcgcatgat caacgccctt tggcgaaacg
     1801 tcctgtaccc aaaaataaac tgacggatca agaacgcgaa aaaataatag aaaccgtttc
     1861 aaaaaaagaa tttatgaatt tgccacctag ccaaattgta ccaaaacttg cggattgctc
     1921 gatttatatt gcatcagagt ctagcttcta tcgtgtattg cgagaaaaaa atatgcaaca
     1981 tcaccgtggc cgaagtcaag tttcacaaaa acgcataccg ccaagtcacc ttgcgacgaa
     2041 gccaaatgaa gtttggactt gggatattac ttggctaaaa ggtccaataa aaggactctt
     2101 ttaccggtta tatctcatca ttgatttatt tagccgtaaa atggtaggct gggagatttg
     2161 ggaatcagaa gaagctcgtc atgccgaaaa acttattaaa cagacggtgc ttaaggaacg
     2221 tatccaagga cagccgttgg ttttacactc cgataatggc agccctatga aagccgcgac
     2281 ttttcaagtc ttactcgaaa aattaggaat tcaaagttct tattcaagac cacgagtgag
     2341 taatgacaat ccttattcgg aagcggcttt ccgaacgtta aaatatcgac cagaatatcc
     2401 caataacggc tttaaaacaa tagatgatgc tcgaaaatgg acgaaagagt ttgtcgattg
     2461 gtataacgat tctcatttgc atagtgggat tcaatttgtg tctcctgcag cgtgtcatga
     2521 aggacgacat ctcgctattt tagagaaacg aaagcaggtt tacgaggacg ctaaaaagcg
     2581 ccatcctgaa cgctggtcaa aaggtgttcg aaattggact ccacatcaat cggtggcgtt
     2641 gaatccttta aaagaaacag aaagttaaca ggagcataat ctttcaagat tatcaaataa
     2701 ccctcgtcac ccaatagggg aaaaagttat tcttccccta ttggataacg aggccaaccg
     2761 accataggga ggttctgctt tttgataggt ggaaaataaa aatacgacaa ctatattgac
     2821 aaacaccgac catcgtccag taaagagacg caataaattc tatcgaagtt tacgcactgc
     2881 ctctac
//
LOCUS       DACDXO010000134         2739 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E23605_2017
            SAMN12639989-rid9881463.denovo.142, whole genome shotgun sequence.
ACCESSION   DACDXO010000134 DACDXO010000000
VERSION     DACDXO010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639989
            Sequence Read Archive: SRR10023389
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2739)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2739)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741914.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:42:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,972
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 33 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 17 of 73
            Pseudo Genes (multiple problems)  :: 10 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2739
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639989-rid9881463.denovo.142"
                     /isolate="E23605_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            700..2610
                     /gene="ltrA"
                     /locus_tag="IWM35_002741"
     CDS             700..2610
                     /gene="ltrA"
                     /locus_tag="IWM35_002741"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAP9920550.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMTNGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWQENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKAKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKNQKDKLVSQAKKI
                     ASPSKGKTELEETRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFTNRLGTNKRG
                     RLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKPMAKVYLANCYTLEG
                     RKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGKCAITLEEFQQANEI
                     HCHHIVPTGAGGNDDYKNLILVKEAVHRLIHAKTEETIQKYIALLKLNKVQLIRLNKY
                     RVLAGNQELNLI"
BASE COUNT         1072 a          425 c          558 g          684 t
ORIGIN      
        1 aaaagctaag gcactaataa aagaacgaaa aagtttggtt attaggagaa ctgataatct
       61 gacaggtgga atgatgaagt aacgctctga aacgcctgcc ctgagcctcc gacaagcatt
      121 gtatgaggaa actcagaatg tttgaagctc ggtaaagacg gctgaaagat accgtaatag
      181 ttgatgaaca tatatcttct atcgaaaact atccagaggt gggtagcgta tccaacaggt
      241 cgggggtcta taaaatatct atggttagaa tgttctataa ggaactgacg aaacagcgaa
      301 tgtacgggtc taaatttgaa ctttgtagaa atgcaaaggt acgagaaatc gtagctggtg
      361 tggcaaagta agaatatagg ttatgaaatg ccatataacg ttacaggcgg tatcaagcca
      421 gcaggcttat agctagtgac ctaaggatat atatgtatag ataagattat cggaacgagg
      481 aaaggtattg ggttctcatc aagagaataa actgacgaag aacaataagc agttgaacta
      541 atgctgaaaa gcagagggcg aacctaagaa attcttgtaa tgagaatgga ggaatgccct
      601 caagtcgtgt atatattaga aaagtattat gttgtttcaa taaggattac gagtaagata
      661 aaaaagctca ctcaatttca agtaaaggag tgaagcctta tgcctaaaaa taaaaatgaa
      721 acattgtgtg tagaagattt aagacacgct gaatattacg aaatgcaaaa cacctttgat
      781 gatttatatg ccaaaagtaa gaatggagat attttcactc atcttatgga tattatccta
      841 tcgagagaaa atatattact ggcatatcga aatatcaaag caaatgcagg cagtaaaaca
      901 gcaggaacag acggtacaat aattaaagat attggcaaat tgccagcaga aacagttgtc
      961 aaaaaagtaa gatacattgt tgcaggcagt ccacatgggt atcgacctaa gccagtgagg
     1021 cgaaaagaaa taccaaaacc aaatggtaaa accagacctc taggtattcc ttgtatgtgg
     1081 gacaggctta tacaacaatg tataaaacaa gtattagaac caatctgcga agctaaattt
     1141 agtgaaaaca gctatggttt tagacctaat aggtcagttg aaaatgcaat aaaagcaacc
     1201 tataaccgcc tacaaatcag ccagctacat tatgtgattg aatttgatat taagggtttc
     1261 tttgacaacg tgaaccattc caagctgata aaacagatat gggctatggg aatacgggac
     1321 aagcacttga tattcatttt aaaaaggata ttaaaagctc caatcaaaat gactaacggt
     1381 acaataacat atccagagaa aggaactccg caagggggaa taatatcccc actactagca
     1441 aacattgttc ttaatgagtt agaccattgg gtagaaagcc aatggcagga aaatcctgtt
     1501 acaaaaaact atgtggttca tatcaataaa agcggtagcc catgtaaaag caacgcttac
     1561 aaagaaatga agaaaacaaa gttaaaggaa atgtatatgg ttcgttatgc agatgatttt
     1621 agagttttct gccgctacaa ggaaagtgct gaaaaagcca aaatagcaat cactcaatgg
     1681 attgaacaac gattaaaatt ggaagtatca caagaaaaaa caagaattgt aaatgttaga
     1741 aaaagatact ccgactttct tggtttcaag attaaaatga taccgagaag aaagaagtta
     1801 gttgttaaat cacatataag tgataaacaa ttcaaaaacc aaaaagacaa gctggtatca
     1861 caggcaaaga aaattgcttc tccctcaaaa gggaaaacgg aattagaaga aacaagactt
     1921 tataattcaa tggtattagg tatgcaaaat tattatcaga ttgcaacgaa tgtaaatctg
     1981 gattgtatga aactgaatag agctgtaatg gctatcttca ctaacagatt aggtacaaac
     2041 aaaaggggca gattgagaaa aacaggcaga caacttacga caaaggaggc aacaagatat
     2101 ggtaaatcta aaatgcttcg gtatgttgct ggcgagggcg aacctatttt ccctgttgga
     2161 tatatccaac acagaaaacc aatggcaaag gtgtatttag cgaattgcta cactctagag
     2221 ggaagaaaat ttatccacac caatctaagt gttgataaat tactgatgta ccagttaatg
     2281 aaattatctt tggaaaatcg cacaatagaa tacgcagaca atcgaatttc gctattttct
     2341 gctcaacatg gaaaatgtgc tataactctg gaagaatttc aacaagctaa tgaaatacat
     2401 tgtcatcata ttgtaccaac tggagctggt ggaaatgacg attataaaaa tcttattttg
     2461 gtaaaagagg cggtacaccg tctaatacac gctaaaacag aagaaacaat acaaaaatat
     2521 atagctctat tgaagctaaa taaagtccag ctcataagac taaataaata tagagtatta
     2581 gctggcaatc aagaattaaa cttgatatag gtctactaca taaatacttt tcaatataaa
     2641 cacgatggaa cgcggagtgc ggtgaaagtc gcacgctccg tgtgaatggg gggaaaagtt
     2701 tgagatattt ataatatcag gaacttacct atccatata
//
LOCUS       DACDXO010000135         2668 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E23605_2017
            SAMN12639989-rid9881463.denovo.143, whole genome shotgun sequence.
ACCESSION   DACDXO010000135 DACDXO010000000
VERSION     DACDXO010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639989
            Sequence Read Archive: SRR10023389
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2668)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741914.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:42:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,972
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 33 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 17 of 73
            Pseudo Genes (multiple problems)  :: 10 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2668
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639989-rid9881463.denovo.143"
                     /isolate="E23605_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..318
                     /locus_tag="IWM35_002742"
     CDS             <1..318
                     /locus_tag="IWM35_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9920551.1"
                     /translation="KAIIPENLDEVKLYLWEDNGEIVGFSGVSAEELVMLFLDPDFIG
                     KRYGSQILIELIETEDIKRIDVNSQNKYAKHFYLSHGFEIESEEDLDGFGKPYPITHL
                     VKK"
     gene            732..1550
                     /locus_tag="IWM35_002743"
     CDS             732..1550
                     /locus_tag="IWM35_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAP9920552.1"
                     /translation="MNKNPHNLKLVAVDMDGTFARTDYTYDIPRFKAVLSRMKAVDCQ
                     FVVASGNQYYQLRSLFPNYYDELSFVAENGAFVKDKTEVLFSADMPRKAIEVVLDLCK
                     DYPEVKNVMCGLNSAYCERGKVSQAFFDLTNIYYHRLEWVADFAEVQDKILKFAPTMP
                     VEKTEFYVELFREKLAGLLVPTSSGHGSVDLILSECHKTFGLKMLVERLGINPDQCVA
                     FGDGGNDIEMLEYCGLSYEMDNATEAVKQVAKHQCPSNDEDGVLVTLDRLFPNT"
     gene            complement(1623..1982)
                     /locus_tag="IWM35_002744"
     CDS             complement(1623..1982)
                     /locus_tag="IWM35_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAP9920553.1"
                     /translation="MSDNLFLEVQPEVFKQVPRLKDGFLLTLNDGSNQFSSAEGCCMI
                     GDHFLLVSTQHYIPAYDLTIPCEEATVKTSEYDTTFINGHLVLEVTPSNQTIRLKNES
                     GILDSNVQVYFDSSDAS"
BASE COUNT          924 a          372 c          471 g          901 t
ORIGIN      
        1 aaagcaatca ttcctgagaa cttagatgag gttaaacttt atttatggga agataacgga
       61 gaaattgtcg gttttagtgg tgtaagtgca gaagaattag ttatgctatt tcttgatcca
      121 gattttattg gtaagcgata tggtagtcaa atattaattg agctgattga aactgaagat
      181 ataaaaagaa ttgatgttaa tagtcaaaat aaatatgcaa aacattttta tttaagccat
      241 ggatttgaaa ttgaatctga agaagactta gatggttttg gtaaaccata ccctataacg
      301 catttagtaa aaaaataatt catgaatggt ttattacctg attgaacatg aataaaagta
      361 atatttttat tttgatggcc tatttctagg ttatgaatta tccagatcaa atttgtgtat
      421 tacattcagt aataaggtgc ttatcttagg atcaacttat gatactagct gaccaaggat
      481 gtaccagtta tagaaaatca gttaaagtaa aaatgataaa aatcttatgt caacactgat
      541 ttaggctgac ctgtgggtta ggagaaagac aggtcggtag cttttaatac aaaaaatggc
      601 ttgtatgtat ctagatagtt aactgaaatt agaaatgttc ttatttttaa cggagtaaga
      661 aaaataggct gctttaggtc tgaatacttt ttcaatataa tgaagatatc aaaggcgaca
      721 ggagtgtttt tatgaataag aatcctcata atctaaagtt ggtagcagta gatatggatg
      781 gaacatttgc tcgtactgat tacacatacg atatcccaag gttcaaggct gttttatcgc
      841 gaatgaaagc tgttgattgt caatttgttg ttgcaagtgg aaatcaatac taccaattga
      901 gatcgttatt tccaaattat tatgatgaat taagttttgt tgctgaaaat ggtgcttttg
      961 ttaaagataa aacagaggta ctattttcag ccgatatgcc tagaaaagcg atcgaggtgg
     1021 tccttgattt atgtaaagac tatcctgaag taaaaaatgt gatgtgtggt ctgaatagtg
     1081 cctactgtga aagaggaaag gtgagtcaag cattttttga tctaaccaat atctattacc
     1141 atagattaga atgggtagct gattttgcag aagttcaaga taaaatttta aaatttgctc
     1201 cgactatgcc tgttgaaaaa accgaatttt acgttgaatt gttccgtgaa aaattagccg
     1261 ggctattagt acctaccagt agtggtcatg gttctgtaga cttgattctt tctgagtgcc
     1321 ataaaacttt cggcttgaaa atgttagttg agcgcttggg gattaatcct gaccagtgcg
     1381 ttgcttttgg agatgggggc aatgacattg agatgttgga gtattgtggt ctgagttatg
     1441 aaatggataa tgcaacggaa gctgttaagc aagttgctaa gcatcaatgt ccttcaaatg
     1501 acgaagacgg tgttttagtg acgctcgatc gattgttccc taacacgtaa acactaggta
     1561 attcatcaaa aaacctcgat ataagaaata ttcgaggttt taaaactata tttagaacag
     1621 atttacgaag cgtctgatga gtcaaaatag acttggacat tgctatctaa aataccactt
     1681 tcattcttta aacgaattgt ttgatttgat ggagttacct ctaatactaa atgcccatta
     1741 ataaaggtag tatcgtattc agatgtttta actgttgctt cttcgcaagg tattgttaaa
     1801 tcatatgctg gaatataatg ttgtgtggaa actaataaaa aatgatcacc aatcatacag
     1861 catccctctg ctgaagaaaa ttgattagag ccatcattta aagttaacaa gaatccatcc
     1921 tttaaacgag gtacttgctt aaatacttct ggttgtactt ctaaaaataa attatcgctc
     1981 attttcacga ccaccatcga cttccacagt tgaaattgac attttattta acacacctat
     2041 tcccccttaa ctataactca attctatata aaaaatattc tattctcaaa taattgaact
     2101 tttcaaatcg ccaatttact tcggctttat cagagctcat tcatatcaag agcgattaac
     2161 gctgtaaaaa ttaaaccgaa gatctgataa attttctcta ttttctacaa tatttaaaac
     2221 gcttccttag tctattatat atctatttct cttaggagta tgtttagatg tttgaataat
     2281 agttgcccaa ctttaaaggt tattcttata atattttact tcattttcca atagaaaatt
     2341 caaaggatta ctggtttttc taaaatagtt tagtttaaat acataagtat cctccgacac
     2401 tgtattagtt cataatcatc taaatacaaa attttcaaat aaaaatattt caagctctat
     2461 cgttgacgaa tgtaaacgat acttataaag taatagaaat tgattacatt tgtaaactta
     2521 aaatggttaa tttatctcac ataaaactaa gaataaaata atgattaaaa gtggttagtt
     2581 tttatacgat ttagcctatc ggtaaaaatg aattagagag tgtaaaatat tttgtgtaaa
     2641 tgaaaaaatc catacaaaaa aggaagtc
//
LOCUS       DACDXO010000136         2505 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E23605_2017
            SAMN12639989-rid9881463.denovo.144, whole genome shotgun sequence.
ACCESSION   DACDXO010000136 DACDXO010000000
VERSION     DACDXO010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639989
            Sequence Read Archive: SRR10023389
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741914.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:42:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,972
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 33 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 17 of 73
            Pseudo Genes (multiple problems)  :: 10 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2505
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639989-rid9881463.denovo.144"
                     /isolate="E23605_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            39..656
                     /locus_tag="IWM35_002745"
     CDS             39..656
                     /locus_tag="IWM35_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9920554.1"
                     /translation="MGFQVLFGSLILTIVFLKWKTSNKKSMWSLVGIVLIIFTVTAAT
                     EANYSHKIQSYKGRLVMTKKDVTRYTSSRTGGLYDYAPLDALKYKDHLKKHEVKVGDR
                     WENASHKAYNSKIVYTVEDADEQNITLPVFNYWGTVVKVNGKKSTIENDNGLVSIKGT
                     GKRTIVEVSSTYPTTMILSLLISSITFIFLFTRYLIRCCGEMKID"
     gene            1676..>2505
                     /locus_tag="IWM35_002746"
     CDS             1676..>2505
                     /locus_tag="IWM35_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAP9920555.1"
                     /translation="MPNFTTEIMETLINKGDLDELFRCHLELAVNSLLQAELTAFLDY
                     EKYDRAGFNSGNSRNGNYSRSFRTEYGELNLVIPRDRNGKFSQQTLPAYKRTNDSLET
                     TIIQLFQKGITMSEISELIEKMYGHHYTPQTISNMTKIVSEDIIAFKERSLESRYSVI
                     FMDATHIPLKRQTVSKEAVYIVIGIRLDGTKEVLGFSLAPTESAYVWKEILQDLKDRG
                     LKEVLLVVTDGLSGINDSIHSVYPNAQFQQCCVHISRNIAHKVRVSDRQEICSDFKLV
                     "
BASE COUNT          914 a          334 c          403 g          854 t
ORIGIN      
        1 gcgtttgatt ttcaaacttc gcaacagaac cgagaacctt gggttttcaa gtgctttttg
       61 gttcattaat cttaaccata gtgtttttaa agtggaaaac aagcaacaaa aaaagtatgt
      121 ggagccttgt aggaatagtt cttattattt ttactgtgac tgcagcaaca gaagcaaatt
      181 attcgcataa aattcagtcg tataaaggtc gtttagttat gaccaaaaaa gatgttacac
      241 gttatacatc ctcaagaact ggaggcctat atgactatgc accattagat gcattgaaat
      301 ataaagatca tttaaagaaa catgaagtaa aagttggtga tcggtgggag aatgcatctc
      361 ataaagcata taattccaaa atagtctata cagtagaaga tgccgatgag caaaatatca
      421 cattacctgt attcaattat tggggaacag ttgtgaaagt aaatgggaaa aagagcacta
      481 tagaaaatga caatggttta gtgtctatta aaggtacggg gaaaagaact atagttgagg
      541 tttcatctac gtatccaact acgatgattc tatctttatt aattagttca ataactttta
      601 tattcttatt tacacgttat ttaataagat gctgcggtga aatgaaaata gattagatag
      661 atattataaa tatgttgatt ctattttcat agcaatggag aaaatacatt agcaatatat
      721 aaaagaaggt atcaagagtc acagtgaatt tattccgaaa agtgaaaaga gcaaatataa
      781 aatttaatga aaatatagaa ctaaatagga aaacgtagac gtaattttct acgttttcct
      841 tttcatatat attaaatatt acggaaaatt atataaacta atttagtgtt ttgtttgtaa
      901 ttcctaattt tgctccactt ttagataatt tttaggtcta tttaactcat cagacattct
      961 atgctcattt ttctccaaat ttgtttcaac tctttcgtac ttaattttgc gtatttgaaa
     1021 attcttcttt tagagtactt ttattacgat actagtatga tatatatcta tagcaaacag
     1081 ttaaaatctt cctaaaataa gcgaatagca ttcctaaaac ctataaaatt tttagatgaa
     1141 gcgacaagtg gacaattaat aaactacttg aacatgaaat tatggatcac ctttcaaatt
     1201 tagaaaatgg aattcttata cttattgcgc aacatttatt gattgcaaaa ttatgtgaca
     1261 aaaaaaacta gtagaagaaa gaataatggc ttgataataa agtactacct attaaaggta
     1321 gtactttatt taatttagta taaattaaca tactactata ttttaggttt catattatat
     1381 aaattaataa tatactacca tttgttggta gtataattta aaggtaataa gttattcttt
     1441 tattactatg tttgggtttt atagcaaaga aagtttaata agagtttgga cctagcaaac
     1501 aatattaatg aggtcattaa atcttattat ctttagtgat ataacgaaat atctacacag
     1561 acaaaatgat agcgtaaaat attttgtgta aatagatatt tagggtagaa aaaaagaaag
     1621 tccattctgt aaaattaaag ttaccacaac cgaaatttta taggaggact ttctcatgcc
     1681 caattttact acagaaatta tggaaacact aatcaataaa ggtgatttag atgaattatt
     1741 tcgatgtcat ttagaattag ctgttaattc attattgcag gctgaattaa cagcttttct
     1801 tgattatgaa aaatatgatc gtgctgggtt caattcaggt aattcccgta atgggaatta
     1861 ttcacgttca ttcagaacag agtatggaga attaaatttg gtaattccta gagatagaaa
     1921 tggtaagttt tctcaacaaa ctttacctgc ttataaaaga accaatgact ctttagaaac
     1981 cactattatt cagctatttc aaaaaggaat tactatgtct gaaatctctg aattgattga
     2041 aaaaatgtac gggcatcact atacgccaca aaccatttca aatatgacta aaatagtttc
     2101 tgaagatatt attgccttta aagaaaggtc cttagaatcc agatactctg tcatttttat
     2161 ggatgctact cacattcctt taaaaagaca aactgtttca aaagaagcag tttatattgt
     2221 aattggcatt cgattagatg gaaccaaaga ggttcttggg tttagtcttg ctccaactga
     2281 atctgcttat gtttggaaag agattcttca agacctaaaa gaccgtggtt taaaagaggt
     2341 tttattagtc gtaactgatg gtttaagtgg cattaacgat agtattcata gtgtttatcc
     2401 aaatgctcaa tttcaacaat gttgtgtcca tatatctaga aatatcgctc ataaggttcg
     2461 tgttagtgat cgacaagaaa tctgtagcga tttcaaatta gttta
//
LOCUS       DACDXO010000137         2453 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E23605_2017
            SAMN12639989-rid9881463.denovo.145, whole genome shotgun sequence.
ACCESSION   DACDXO010000137 DACDXO010000000
VERSION     DACDXO010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639989
            Sequence Read Archive: SRR10023389
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741914.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:42:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,972
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 33 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 17 of 73
            Pseudo Genes (multiple problems)  :: 10 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2453
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639989-rid9881463.denovo.145"
                     /isolate="E23605_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            132..344
                     /locus_tag="IWM35_002747"
     CDS             132..344
                     /locus_tag="IWM35_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014834536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9920556.1"
                     /translation="MAVWQIQLKERGAYGLISLSDVLCRKEMPDLFSLDQIIAIIGAA
                     AVVITAVAKASDSILAWLKFKKEQKK"
     gene            506..1024
                     /locus_tag="IWM35_002748"
     CDS             506..1024
                     /locus_tag="IWM35_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000357244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HAP9920557.1"
                     /translation="MDKFNRLTLINQFEILKALNPNEEKYYAEKIEILTEGYEYHYSE
                     IFENISDPLPEEDSRFVLDILNMYRDITFSKNKLKDINSIDNYYIQFKGFDFNSSTEF
                     KLALYAQFFIEKLNRFQEIVEDSDFNTFNSHKPMRGTYIKFLENYNDLKSTNNYQFGN
                     LSYEMISKVLSL"
     gene            1031..1543
                     /locus_tag="IWM35_002749"
     CDS             1031..1543
                     /locus_tag="IWM35_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9920558.1"
                     /translation="MKLNSINDLFAIIGVIANILFSCLNFRFTQKNKKNKLSIEQMKK
                     DIETDLKNDHKERRKIEEYTSLMEKCKATDAIFSFLRKSDEIITRENIDLENEISKLE
                     SIFSDINSLKYLIVEENSDSFLEFEKYLTDLIDNRNNELMRQNIARFLTKLVSEWKSI
                     ISQNMDKLRY"
     gene            1814..2437
                     /locus_tag="IWM35_002750"
     CDS             1814..2437
                     /locus_tag="IWM35_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP9920559.1"
                     /translation="MYLAYLRVSSDDQSLARQHKLIENWSVKNNIAEDDLKIFEEKVS
                     GKNIENRPELQELISFIRERDTIIIPSLDRLARNSKDTKDLLQLFRSKGASIEILDLP
                     SFNGVTDPALRDLLTNLVIEVFSYVAENERKKIKERQKQGIEIAKKNGRYTGKKIQYH
                     ANATGKNKLIYDEIVDGLKKKETISKIAKNAGVTRKTVYRVKRDLGM"
BASE COUNT          943 a          310 c          408 g          792 t
ORIGIN      
        1 aaaacactat atattccgaa aagtgagtat ctccattcat aaaataatca acatatttaa
       61 aaaatctaat tagctttttt agtcaatcta aagttcttta ttctatgata taatgttact
      121 gtagaaagtt tgtggcggtt tggcaaatcc aattgaagga gcgtggtgcc tatgggctta
      181 ttagcttatc ggacgtactc tgtagaaagg aaatgcctga tttgttctca ttggatcaaa
      241 tcattgctat tatcggagca gcagcagtag ttattactgc cgtagcgaaa gctagcgatt
      301 cgattttagc ttggttaaag ttcaaaaaag aacaaaaaaa atagagccgt ctcaactttt
      361 ggcgaggtag acgagctcta aagctttatt tacatgccat ccgctttgaa cggactacac
      421 tgggcatgtg acagcatgcc ctttcattta tattatatct cattctttta tataatgaaa
      481 gtaaataaat catggaggat aaaatatgga taaatttaat agattaacat taataaatca
      541 gtttgaaatt ttaaaagctt taaatcctaa cgaagaaaaa tactatgctg aaaaaataga
      601 aatactaaca gaaggttacg aatatcacta tagtgaaata tttgaaaata tttctgatcc
      661 attgccagaa gaagattcgc ggtttgttct agacatatta aatatgtata gagatattac
      721 ttttagtaaa aataaattaa aagatataaa ttcaattgat aattattata tacaatttaa
      781 aggttttgac tttaatagct ctaccgaatt taagttagca ctttatgcac aattttttat
      841 tgaaaaatta aataggtttc aagaaatagt tgaagatagt gattttaaca cttttaatag
      901 ccataaaccg atgagaggta cttatataaa atttctcgaa aattataatg atttaaaatc
      961 taccaataat tatcaatttg gaaacttgtc ttatgagatg ataagtaaag ttctaagttt
     1021 gtgatgataa atgaaattaa atagtataaa tgatttattt gctattatag gtgttattgc
     1081 taacatactt ttttcgtgtt tgaattttag atttactcaa aagaacaaaa agaacaaact
     1141 cagtatagaa caaatgaaaa aagacattga aactgattta aaaaatgacc ataaagaaag
     1201 aagaaaaatt gaagaatata cttctttaat ggaaaagtgt aaagcaactg atgcaatttt
     1261 tagcttttta agaaaaagtg atgagataat cacacgtgaa aatatagatt tagaaaatga
     1321 gattagtaaa cttgaatcta tcttttccga tataaattct cttaagtatt taatcgtcga
     1381 agaaaattcg gatagctttt tagaatttga aaaatatcta acagatctta tagataatag
     1441 aaataatgaa ttaatgagac aaaacattgc aagattcctt actaaattag tttctgaatg
     1501 gaaaagtatt atttctcaaa atatggataa attaagatat taaggatgtt agttttaaaa
     1561 taaactaatg tccttatttt tgtgttcaca tttctgttac ctaagaaata cttaaaggag
     1621 gtgatgtagc cagcaacgac tacaacttga acccgctatc tacagtttat ttgttgtttc
     1681 ttttgggtat actctaatgg tacaaatcca tatttgctta aagcgttgat ttttcagcgg
     1741 tttttcttta ttgcaaagtg taccctaaga cctgtatact aatagtaaat agattggata
     1801 ggaatgatgg caaatgtatt tagcttattt aagagtttct tccgacgatc aaagtttagc
     1861 tcggcaacat aagctaattg aaaattggtc agtcaaaaat aatattgctg aagacgattt
     1921 aaaaattttc gaggaaaaag tctctggaaa aaatattgaa aacagaccag agctgcaaga
     1981 gcttatttct ttcatacgtg aacgagatac gattattatt cctagtcttg atcgattagc
     2041 aagaaatagt aaagatacaa aagacttact tcaattgttt cgtagtaaag gtgcttctat
     2101 tgaaatatta gacctaccgt cgtttaatgg ggtaactgac cctgcattaa gagacttgct
     2161 aacaaattta gtcatagaag tgtttagcta tgttgctgaa aacgaacgga aaaaaatcaa
     2221 agagcgtcaa aaacaaggca ttgaaattgc aaagaaaaat ggtcgttata ctggtaaaaa
     2281 gattcaatac catgccaatg caacaggaaa aaacaaattg atttacgatg aaattgttga
     2341 tggtctgaag aaaaaagaaa ctatttctaa aatagcaaag aacgcaggag ttacaagaaa
     2401 aaccgtttat cgagtaaaaa gagatttagg aatgtaatat gttccagtta gaa
//
LOCUS       DACDXO010000138         2398 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E23605_2017
            SAMN12639989-rid9881463.denovo.146, whole genome shotgun sequence.
ACCESSION   DACDXO010000138 DACDXO010000000
VERSION     DACDXO010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639989
            Sequence Read Archive: SRR10023389
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741914.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:42:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,972
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 33 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 17 of 73
            Pseudo Genes (multiple problems)  :: 10 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2398
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639989-rid9881463.denovo.146"
                     /isolate="E23605_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            207..2069
                     /locus_tag="IWM35_002751"
     CDS             207..2069
                     /locus_tag="IWM35_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP9920560.1"
                     /translation="MQQLTKRERQLLIYLVEMSEPVTIKKLAEKENVSVRTIKYDLDD
                     IREWLNERKQVLYSKRSQGIWMNISSSDRIKLKSELMDVNRFEIFADQSLRIKRLLIM
                     LLVTKEAITGMKMANALDVSKDTIMNDLDVLENNFLKEGLTLNRQARKGFWIIGEERL
                     VRLTIEEILQKEFTDYDIYKLMSLLLNGGETEYFEMYSATATPIQEVFNQVIIRMRHL
                     LEFENLEKLNYAELLNILIRVTIATVRLRKEATIGRYQLVAEQELDKNDLSYRVMQQV
                     FDYYQLPLFEDEYHYIYSDTFDTIHQQDVMKLVENVIYKVSKAINHSFYQDPQLLTNL
                     YAHLSMRLSRKKKFVNEYNPFKEDIKQKYPELFDAIKQAVRQEISEQNILINDSFVAY
                     IALHFLVSYEREEDLRSVRAVYICSTGLGVTSLIKQKINEELNNITIVAFASVLNAQE
                     MIATKDPDLVISIFPINGIDRPFIKVHPLPTEQDLKKIQQIIKKILASSNQQERHNLN
                     LRETSINKDTLEDFSKELILKAYTIYERLLVILEAELIQEYREAFLLHVFLMVHRITF
                     DQQYMAEGRQVDQIILDKGTLVGKIEAVFAENNLGINQSEISALFSYIKEGASS"
     gene            2069..>2398
                     /locus_tag="IWM35_002752"
     CDS             2069..>2398
                     /locus_tag="IWM35_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP9920561.1"
                     /translation="MKMSDEALKIIEESQNREKLKQVIDLIEERLVEHAIVPTELQWT
                     ILINHLNEMLKRSQRKETIPVVDRDLFKEVSKEAITISDEIVRSIGNLTDDEIYVLSI
                     HFETAKNN"
BASE COUNT          865 a          353 c          466 g          714 t
ORIGIN      
        1 aaaaatttga aaaagcgttt gattatttat attacgactt agtgcaataa aaaacgcttt
       61 ttaacagtac atgtagtcgt ctttataata gataaactct gtaatattag ttaaaaagct
      121 ataagttgca cttagttaag tgcaaacatt tcatattgtc gttagtttag aaaacgctta
      181 tattagcaat gtaaggagag atgataatgc agcagctaac aaaaagagaa aggcagcttt
      241 tgatttactt ggtggaaatg tctgaaccgg taacaatcaa aaagttggct gaaaaagaaa
      301 atgttagtgt tcggacgata aaatacgatc tagatgatat aagagagtgg ctgaatgagc
      361 ggaagcaagt cctatatagc aaacgtagtc agggaatttg gatgaatatt agtagctctg
      421 atcgaatcaa attaaaaagc gaattgatgg atgttaatcg ttttgaaata tttgcagatc
      481 aaagtctccg aatcaaacgt ttactaatta tgcttttagt aacaaaagaa gctattactg
      541 gaatgaaaat ggccaacgca ttagatgtca gcaaagatac gatcatgaat gatttagatg
      601 tgctggaaaa taattttctt aaggaaggat tgaccttgaa tcgtcaggcg cgaaagggtt
      661 tttggatcat aggagaagaa cgtctagttc gattaacgat tgaagagatt cttcaaaaag
      721 agttcacgga ttatgacatt tataaattaa tgtcattgtt attgaatggc ggtgagactg
      781 aatactttga aatgtattct gctactgcta cacctatcca agaggtattt aatcaagtaa
      841 tcattcgaat gcgacactta ctggaatttg aaaatctaga gaaactgaat tacgcggaat
      901 tactaaatat tctgattcga gtgacaattg ctaccgttcg cttacgcaaa gaggctacga
      961 ttggtcgtta tcaattggtt gctgaacaag aattagacaa aaacgatctt tcttatcgcg
     1021 tcatgcagca ggtctttgat tattatcagt tgccactgtt cgaggatgaa tatcattata
     1081 tctatagtga tacattcgac acgattcacc agcaagacgt catgaaacta gttgagaatg
     1141 tgatttataa agtaagtaaa gcaatcaacc attcttttta tcaagatcca caattgttga
     1201 cgaatctgta tgctcattta tccatgcgat tatcgagaaa gaaaaaattt gtcaatgaat
     1261 ataatccttt taaagaagat atcaagcaga aatacccgga attatttgat gcgatcaagc
     1321 aagcagttcg acaagaaata agtgaacaaa atattctgat caatgattct ttcgtggcat
     1381 atattgcctt acactttttg gtttcctacg aaagagaaga agatctacga agcgttagag
     1441 ccgtctacat ttgttcaaca gggttgggtg tgactagtct gatcaagcaa aagatcaatg
     1501 aagaattaaa taatatcact atcgtggcat ttgcttcagt attgaatgcg caagaaatga
     1561 ttgctaccaa agatcctgat ttggtgatta gtatttttcc aattaatggc atcgatcgtc
     1621 ctttcatcaa agtccatcca ttacccactg aacaggactt gaaaaaaatt caacaaataa
     1681 taaaaaaaat acttgcttct tccaatcagc aggaaaggca taacttgaat cttagagaaa
     1741 cgtccatcaa taaagatact ttagaagact ttagcaagga attgattttg aaagcttata
     1801 cgatttatga acgtttactt gtcattttgg aagcagaatt gatccaagaa tatcgagaag
     1861 cttttttgct tcatgtattc ttaatggttc atcgtatcac atttgatcaa caatatatgg
     1921 cagaaggtcg tcaagtagac cagattattt tagacaaagg aacactggta ggaaaaatcg
     1981 aagcagtttt tgcagaaaat aatttaggaa tcaatcagtc agaaatcagt gcattattct
     2041 catatataaa agaaggagcg tcaagttagt gaaaatgtca gacgaggctt taaaaattat
     2101 cgaggaaagt caaaatcggg aaaagttaaa acaagtgatt gatctaattg aagagcgatt
     2161 agtagaacat gctattgtac caaccgagtt gcagtggacc attctaatta atcacttgaa
     2221 tgagatgctg aaacgttctc aaagaaagga aacgattccc gtagtcgatc gggatttgtt
     2281 caaagaagta tccaaagaag ccataactat ttctgatgaa atagttagaa gtatcggtaa
     2341 tttaaccgat gatgaaatat atgttttgtc gattcatttt gaaacagcaa aaaataat
//
LOCUS       DACDXO010000139         2395 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E23605_2017
            SAMN12639989-rid9881463.denovo.147, whole genome shotgun sequence.
ACCESSION   DACDXO010000139 DACDXO010000000
VERSION     DACDXO010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639989
            Sequence Read Archive: SRR10023389
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741914.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:42:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,972
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 33 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 17 of 73
            Pseudo Genes (multiple problems)  :: 10 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2395
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639989-rid9881463.denovo.147"
                     /isolate="E23605_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..832)
                     /locus_tag="IWM35_002753"
     CDS             complement(<1..832)
                     /locus_tag="IWM35_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP9920562.1"
                     /translation="MKKLKIFPKMFIQIFSVLGIIIILVHSLIFFIFPKTYLETRKEK
                     IHNMANEISSNMNGKEIKYIEQTLEFYSKSSEIKVFIKEKNNKNEIQIKDNINVNLES
                     DSNSLIIEEREIKLNDGKKTNLQFVSTADMQKDAKDLSLKFLPYSLLISILFSAIISL
                     IYAKSIKNNIQEIKIVTDKMMKLDKKMSLKVSSNDEVGELKQQINDLYSTLLRTIDDL
                     EFKNKEILKLEKLKYDFFKGASHELKTPLASLKIILENMKYNIGKYKERDIYINECIE
                     I"
     gene            complement(829..1482)
                     /locus_tag="IWM35_002754"
     CDS             complement(829..1482)
                     /locus_tag="IWM35_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9920563.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFS
                     LPVLKARIDSLIKKNYGHLEKFEYKDLSVNFNSYTAKINGEEIDVNAKELEVLKCLLD
                     NNGQVLTRMQIIDYVWKDSEETPYDRVIDVYIKELRKKLQLDCITTIRNVGYKLERK"
     gene            1770..>2395
                     /locus_tag="IWM35_002755"
     CDS             1770..>2395
                     /locus_tag="IWM35_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP9920564.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGRLDDSP
                     SHCEHCGSIRIIRNGSYTTRTQILKVKEKLTILELKRTRFLCYDCGQTFSAKTDLVDE
                     HHQLTKELKQAILIELYENQSRKLIAKKYFVSDGTVTRILREATKHYQPRMNFLPTVL
                     CMDEFKSMKSVSGSMSFICVDGTTHQLFTILEDRRLYKLTQYFLRFPR"
BASE COUNT          768 a          401 c          293 g          933 t
ORIGIN      
        1 cgatttcaat acattcatta atataaatat ctctttcttt atattttcca atattgtatt
       61 tcatgttttc cagtattatt ttaagactag caagaggggt tttaagttca tgggatgcac
      121 ctttaaaaaa gtcatatttt aacttttcta attttaaaat ttctttattt ttaaattcca
      181 aatcgtcaat tgttcttaat aaagtagaat acaaatcatt aatttgttgt tttaattctc
      241 caacttcatc attagagcta acttttaaac tcattttttt atcaagtttc atcattttat
      301 cagtaacaat ttttatttct tgtatattat tttttattga ttttgcataa attaaagaaa
      361 taatagcaga aaataaaatt gatattaata aagaatatgg taaaaattta aggcttaaat
      421 ctttcgcatc tttttgcata tcagctgtag aaacaaattg tagatttgtt tttttaccat
      481 cattaagctt tatttctctt tcttcaatta tcaaagaatt actatcactt tctaaattaa
      541 catttatatt atctttgatt tgtatttcat tcttattatt tttttctttt atgaatactt
      601 ttatttcact actcttggaa taaaactcca aagtttgttc tatatatttt atttcttttc
      661 cattcatatt actagaaatt tcatttgcca tattatgaat cttttctttt ctcgtctcca
      721 aatatgtttt aggaaagata aaaaaaatta atgaatgaac taatataatt attattccaa
      781 gaacagaaaa tatttgtatg aacatttttg gaaatatctt taattttttc attttctctc
      841 caatttatat cctacatttc ttatagtagt tatacagtct aattgtaatt tttttctaag
      901 ttcttttata tatacatcta ttactcgatc ataaggagtt tcttcgctat ctttccatac
      961 ataatcaata atttgcattc ttgttaacac ttgtccatta ttatccaata aacattttaa
     1021 tacttctagt tcttttgcat ttacatctat ttcttcacca tttattttag ctgtatagct
     1081 attaaaatta accgatagat ccttatattc aaacttctct aaatgtccat aattcttctt
     1141 aattaaagaa tctattctag cttttaagac cggcaatgaa aatggctttt ctacatatcc
     1201 gtctactaaa ttagtaaatg catcaatttt atattcttca tcactaaatg cagtcaagat
     1261 tagaattggt aaattgcttt tctttctaat ttctttcaac acttctaaac catttataaa
     1321 aggtatctga atgtctaaga taaccaaatt tatatcgcta ttaaattttg acaaggcttc
     1381 tcttccatct ttagcttgaa taacagtata tccaaattct gaaagatatt cacttattcc
     1441 ctctcttatc atattatcat cttcaacagt taatattttc atatatacct ccacttaagc
     1501 ctatacacta gatattatac agcatattat ggctctataa tatctagtgt tggtgacacc
     1561 ttctgttgcc aatacttttt cttttttcta ataaatgcaa acagacacct tgaaatcctt
     1621 tagaataaag ggttctataa tattttgtgt tgttggaaaa tcgaaggcga ttttctacta
     1681 acactttttc ttcgtttcaa ctcaaaaaga gactcacttt tggtaaaatt tagtcgacca
     1741 aaacatcacc ctaccaaagg agtcgctcta tggataatca tactagaaaa ttactcggat
     1801 taacagataa acatttattt tttgatgaag aatggctgat tgaaaaagaa tataaaggcg
     1861 ttcaagcgca gtttattaaa gggagattgg atgactcacc ttcccattgt gaacactgtg
     1921 gatcgattcg tattatccgc aacggttcct atacgacacg tactcagata ttgaaagtca
     1981 aagaaaagtt gactatttta gaattaaaac gtacccgttt tctatgctat gattgtgggc
     2041 agaccttttc tgcgaaaacg gacttagttg atgaacatca tcaactaaca aaggaactga
     2101 agcaggcgat tctcatagaa ctgtacgaaa atcaatcacg taaactaatc gctaagaagt
     2161 acttcgtctc ggatgggacg gtgacacgga tcttgcgtga agcaacgaag cattaccagc
     2221 cacgtatgaa cttccttccg acggtgttgt gtatggatga gttcaaatca atgaagtctg
     2281 tttctggttc catgagtttt atttgtgttg atgggacaac ccatcagcta tttacgatcc
     2341 ttgaagatcg cagactttac aaactaactc aatacttcct gcgctttcct agaaa
//
LOCUS       DACDXO010000140         2246 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E23605_2017
            SAMN12639989-rid9881463.denovo.150, whole genome shotgun sequence.
ACCESSION   DACDXO010000140 DACDXO010000000
VERSION     DACDXO010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639989
            Sequence Read Archive: SRR10023389
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741914.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:42:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,972
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 33 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 17 of 73
            Pseudo Genes (multiple problems)  :: 10 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2246
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639989-rid9881463.denovo.150"
                     /isolate="E23605_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(10..81)
                     /locus_tag="IWM35_002756"
     tRNA            complement(10..81)
                     /locus_tag="IWM35_002756"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(46..48),aa:Glu,seq:ttc)
     gene            complement(93..166)
                     /locus_tag="IWM35_002757"
     tRNA            complement(93..166)
                     /locus_tag="IWM35_002757"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(130..132),aa:Ile,seq:gat)
     gene            complement(179..249)
                     /locus_tag="IWM35_002758"
     tRNA            complement(179..249)
                     /locus_tag="IWM35_002758"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(215..217),aa:Gly,seq:tcc)
     gene            complement(254..326)
                     /locus_tag="IWM35_002759"
     tRNA            complement(254..326)
                     /locus_tag="IWM35_002759"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(291..293),aa:Phe,seq:gaa)
     gene            complement(330..402)
                     /locus_tag="IWM35_002760"
     tRNA            complement(330..402)
                     /locus_tag="IWM35_002760"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(367..369),aa:Asp,seq:gtc)
     gene            complement(421..494)
                     /locus_tag="IWM35_002761"
     tRNA            complement(421..494)
                     /locus_tag="IWM35_002761"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(458..460),aa:Met,seq:cat)
     gene            complement(507..596)
                     /locus_tag="IWM35_002762"
     tRNA            complement(507..596)
                     /locus_tag="IWM35_002762"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(558..560),aa:Ser,seq:tga)
     gene            complement(619..690)
                     /locus_tag="IWM35_002763"
     tRNA            complement(619..690)
                     /locus_tag="IWM35_002763"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(655..657),aa:Glu,seq:ttc)
     gene            complement(712..799)
                     /locus_tag="IWM35_002764"
     tRNA            complement(712..799)
                     /locus_tag="IWM35_002764"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(763..765),aa:Ser,seq:gct)
     gene            complement(824..897)
                     /locus_tag="IWM35_002765"
     tRNA            complement(824..897)
                     /locus_tag="IWM35_002765"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(861..863),aa:Ile,seq:gat)
     gene            complement(911..981)
                     /locus_tag="IWM35_002766"
     tRNA            complement(911..981)
                     /locus_tag="IWM35_002766"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(947..949),aa:Gly,seq:tcc)
     gene            complement(983..1058)
                     /locus_tag="IWM35_002767"
     tRNA            complement(983..1058)
                     /locus_tag="IWM35_002767"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1023..1025),aa:Asp,seq:gtc)
     gene            complement(1077..1150)
                     /locus_tag="IWM35_002768"
     tRNA            complement(1077..1150)
                     /locus_tag="IWM35_002768"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1114..1116),aa:Met,seq:cat)
     gene            complement(1163..1252)
                     /locus_tag="IWM35_002769"
     tRNA            complement(1163..1252)
                     /locus_tag="IWM35_002769"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1214..1216),aa:Ser,seq:tga)
     gene            complement(1276..1349)
                     /locus_tag="IWM35_002770"
     tRNA            complement(1276..1349)
                     /locus_tag="IWM35_002770"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1313..1315),aa:Ile,seq:cat)
     gene            complement(1363..1436)
                     /locus_tag="IWM35_002771"
     tRNA            complement(1363..1436)
                     /locus_tag="IWM35_002771"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1400..1402),aa:Met,seq:cat)
     gene            complement(1464..1536)
                     /locus_tag="IWM35_002772"
     tRNA            complement(1464..1536)
                     /locus_tag="IWM35_002772"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1501..1503),aa:Ala,seq:tgc)
     gene            complement(1556..1629)
                     /locus_tag="IWM35_002773"
     tRNA            complement(1556..1629)
                     /locus_tag="IWM35_002773"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1593..1595),aa:Pro,seq:tgg)
     gene            complement(1639..1712)
                     /locus_tag="IWM35_002774"
     tRNA            complement(1639..1712)
                     /locus_tag="IWM35_002774"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1676..1678),aa:Arg,seq:acg)
     gene            complement(1723..1808)
                     /locus_tag="IWM35_002775"
     tRNA            complement(1723..1808)
                     /locus_tag="IWM35_002775"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1772..1774),aa:Leu,seq:taa)
     gene            complement(1825..1896)
                     /locus_tag="IWM35_002776"
     tRNA            complement(1825..1896)
                     /locus_tag="IWM35_002776"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1862..1864),aa:Gly,seq:gcc)
     gene            complement(1908..1980)
                     /locus_tag="IWM35_002777"
     tRNA            complement(1908..1980)
                     /locus_tag="IWM35_002777"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1945..1947),aa:Thr,seq:tgt)
     gene            complement(1998..2079)
                     /locus_tag="IWM35_002778"
     tRNA            complement(1998..2079)
                     /locus_tag="IWM35_002778"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2043..2045),aa:Leu,seq:tag)
     gene            complement(2087..2159)
                     /locus_tag="IWM35_002779"
     tRNA            complement(2087..2159)
                     /locus_tag="IWM35_002779"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2124..2126),aa:Lys,seq:ttt)
     gene            complement(2163..2235)
                     /locus_tag="IWM35_002780"
     tRNA            complement(2163..2235)
                     /locus_tag="IWM35_002780"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2200..2202),aa:Val,seq:tac)
BASE COUNT          598 a          627 c          535 g          486 t
ORIGIN      
        1 tatcttccat gacccgtacg ggattcgaac ccgtgttacc gccgtgaaag ggcggtgtct
       61 taaccgcttg accaacgggc caataaaata aatgggccta aatggactcg aaccatcgac
      121 ctcacgctta tcaggcgtgc gctctaacca gctgagctat aggcccataa aaactaaaag
      181 cgggtgacga gaatcgaact cgcgacaaca gcttggaagg ctgtggtttt accactaaac
      241 tacacccgca taatggcgcg ggacagaatc gaactgccga cacatggagc ttcaatccat
      301 tgctctacca actgagctac cgagccaaac ggttccgacg ggaatcgaac ccgcgatctc
      361 ctgcgtgaca ggcaggcatg ttaaccccta caccacggaa ccaatgtttt tagtttttaa
      421 ttgcgggagc aggatttgaa cctacgacct tcgggttatg agcccgacga gctaccagac
      481 tgctccatcc cgcgataata tgaaaaagga ggataaggga ttcgaaccct tgcacggttt
      541 tacccgcctg acggttttca agaccgttcc cttcagccgg acttgggtaa tcctccatgt
      601 aaaactatat taaattaatg acccgtacgg gattcgaacc cgtgttaccg ccgtgaaagg
      661 gcggtgtctt aaccgcttga ccaacgggcc aggtaaaaca aaatattgaa acggagaagg
      721 agggatttga accctcgcgc cggtttcccg acctacaccc ttagcagggg cgcctcttca
      781 gccacttgag tacttcccca aaaccaataa gattttgtac taatgggcct aaatggactc
      841 gaaccatcga cctcacgctt atcaggcgtg cgctctaacc agctgagcta taggcccata
      901 aaaaactaaa agcgggtgac gagaatcgaa ctcgcgacaa cagcttggaa ggctgtggtt
      961 ttaccactaa actacacccg catggcggtt ccgacgggaa tcgaacccgc gatctcctgc
     1021 gtgacaggca ggcatgttaa cccctacacc acggaaccag tgtttttagt ttttaattgc
     1081 gggagcagga tttgaaccta cgaccttcgg gttatgagcc cgacgagcta ccagactgct
     1141 ccatcccgcg ataatatgaa aaaggaggat aagggattcg aacccttgca cggttttacc
     1201 cgcctgacgg ttttcaagac cgttcccttc agccggactt gggtaatcct ccatatgaaa
     1261 cttacaacgc cacaatggac cttgtaggac tcgaacctac gaccggacgg ttatgagccg
     1321 tctgctctaa ccaactgagc taaaggtcca ggctcttaaa aatcgcggcg gaggggatcg
     1381 aacccccgac ctcccgggta tgaaccggac gctctagcca gctgagctac accgcgaaaa
     1441 agatattcat tatttaaatg aaatggagcc tagcgggatc gaaccgctga cctcctgcgt
     1501 gcaaagcagg cgctctccca gctgagctaa ggccccgtgt ttaaatttat aattatcggg
     1561 aagacaggat tcgaacctgc gaccccttgg tcccaaacca agtgctctac caagctgagc
     1621 tacttcccgt ttaattaacg cgcccaagag gagtcgaacc cctaaccttt tgatccgtag
     1681 tcaaacactc tatccagttg agctatgggc gcatatttga aatgccgagg accggaatcg
     1741 aaccggtacg gtgatcactc accgcaggat tttaagtcct gtgcgtctgc cagttccgcc
     1801 accccggcgc gattgctttg gcaaagcgga aaacggggtt cgaacccgcg acccccacct
     1861 tggcaaggtg gtgctctacc actgagctat ttccgcgtgt ttaaagatgc cggctaaagg
     1921 acttgaaccc tcgaccctct gattacaaat cagatgctct accaactgag ctaagccggc
     1981 atgtaaaact taaattatgc gggtgaaggg acttgaaccc ccacgccgta aggcgctaga
     2041 tcctaaatct agtgcgtctg ccaattccgc cacacccgca aaaatatgag ccgtacaggg
     2101 ctcgaacctg tgaccctctg attaaaagtc agatgctcta ccaactgagc taacggctca
     2161 tatggaggtt aacgggatcg aaccgctgac cctctgcttg taaggcagat gctctcccag
     2221 ctgagctaaa cctccatgga taactt
//
LOCUS       DACDXO010000141         2232 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E23605_2017
            SAMN12639989-rid9881463.denovo.151, whole genome shotgun sequence.
ACCESSION   DACDXO010000141 DACDXO010000000
VERSION     DACDXO010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639989
            Sequence Read Archive: SRR10023389
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741914.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:42:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,972
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 33 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 17 of 73
            Pseudo Genes (multiple problems)  :: 10 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2232
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639989-rid9881463.denovo.151"
                     /isolate="E23605_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..543
                     /locus_tag="IWM35_002781"
     CDS             <1..543
                     /locus_tag="IWM35_002781"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAP9920565.1"
                     /translation="ISKENLLNQDFSTETICEKWAADITYIPTKKNGWCYLSSIMDLH
                     TKRIISYTFSKRMTVDCVIQTLNKAKIHYHIPEGMILHTDLGSQYTAREVEQWLKTNK
                     IRHSYSRKGTPYDNAGIESFHASLKKEEVYTTSYSDFEEANRALFSYIEGFYNRNRIH
                     SSIHYLTPQEFEELAKEKMA"
     gene            complement(899..1471)
                     /locus_tag="IWM35_002782"
     CDS             complement(899..1471)
                     /locus_tag="IWM35_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP9920566.1"
                     /translation="MTKYGYARVSTASQSTKEQIQQLLENCVEKKNIFSEKFTGTKRD
                     RPKFNQLLAGLKPGDEIVVTKLDRFARNTREALEIIEPLLDDNVTIKVLNLGTIENTS
                     MGRMVTRTLLSVAEMERDMIVERTQEGKMFARKNNPNFREGRPKATITPKKRHAYELL
                     TSGKSYKEVEAITGFSRSTLFRIKKKIEES"
     gene            complement(1487..2092)
                     /locus_tag="IWM35_002783"
     CDS             complement(1487..2092)
                     /locus_tag="IWM35_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell filamentation protein"
                     /protein_id="HAP9920567.1"
                     /translation="MILENKLGLTNQVELAKVEEKLSKQKAKELYDCGKINEIEVGTF
                     KGLSEIHEFLFSDIYDFAGKIRSVNIAKGNFRFAPVMYLEHSLQHIDQMPQTSFEEII
                     KKYVEMNIAHPFREGNGRSTRIWLDLILKEELQKVVDWNLIDKADYLSAMERSPINDL
                     EIRYLISNALTDKIDDRELYMKGIDVSYFYEGYSEYTIDDL"
BASE COUNT          701 a          453 c          338 g          740 t
ORIGIN      
        1 atttcaaaag agaatctctt aaatcaggac ttttctactg aaacgatctg tgagaaatgg
       61 gcagctgaca ttacgtacat tcctactaag aaaaatggtt ggtgttactt atcttcaatc
      121 atggatttac acacgaaaag aattattagt tatacatttt caaaacgaat gactgtggat
      181 tgtgtcattc aaacattgaa taaagcaaaa atacattatc acattccaga aggaatgatt
      241 ctacacactg acttgggcag ccaatacaca gcaagagaag tggaacaatg gcttaaaacc
      301 aacaaaataa ggcattctta tagtagaaaa ggaacacctt atgataatgc tggaatcgaa
      361 tctttccatg cctcattgaa aaaagaagaa gtctacacga ctagctactc agattttgaa
      421 gaagcaaatc gagcgctatt tagctacatt gaaggatttt ataaccgaaa tcgaatccat
      481 agctcgattc actatctaac cccacaggaa tttgaagaat tagcaaaaga aaaaatggct
      541 taaccgtctc tactaaagac aatcaacacc gtagggatat ggagataaaa tcaatagagt
      601 caatagatta tttatcgata ataacactaa aaaagccacg ttcctaaacg tgacttacgc
      661 tgaacgatat gtgttgtttg gtagacgaac aaatcgttca ttataaatat atgatggtat
      721 cctaatatag taagcgtggc tagcatacct ttaattactt taccttttac agttaccata
      781 cacatttttt gagcagaaat caagtaaata aaaaaatatt atatttcaca acatgctaat
      841 agactcatat ctagaacgca ttaagtattt acctgtctgg ttcgctgaga cttctttttt
      901 aactttcttc aattttcttc tttattctaa aaagcgtact gcgactgaaa ccggttatag
      961 cttcaacttc cttatacgat ttacctgaag taagcagttc atatgcgtgc ctcttcttcg
     1021 gtgtgattgt tgctttcgga cgtccttctc tgaaattagg attattcttt cttgcaaaca
     1081 tttttccttc ttgggtacgt tccacaatca tatcacgttc catttctgct acactcaaca
     1141 acgtccgtgt caccattcgc cccatggacg tattttcaat cgttcctaga ttcaaaacct
     1201 tgatcgttac gttatcatcg agtaaaggtt caatgatctc taatgcttct ctcgtgttac
     1261 gagcaaatcg atcaagcttc gtaacaacta tttcatctcc aggcttcaga ccagccaaca
     1321 gctgattgaa ttttggccga tcccgttttg tacccgtaaa tttttctgaa aaaatattct
     1381 tcttctctac gcagttctca agcaactgtt gaatttgttc ttttgtactt tgagaagcgg
     1441 ttgagacacg tgcatatcca tatttcgtca tttggtccac ctacttttac aaatcatcta
     1501 tcgtatactc agaataccct tcatagaaat agctgacatc gattcctttc atatataact
     1561 cgcgatcgtc aattttatca gtcagagcat tggaaattag atagcgtatt tccaaatcgt
     1621 ttatgggact tcgctccatt gcagaaagat agtctgcttt atcgattaag ttccaatcta
     1681 caactttttg caattcttcc tttaaaatga gatccagcca aattctagta cttcggccat
     1741 ttccttctcg aaatgggtga gcgatattca tctccacata tttcttgata atctcttcaa
     1801 aagaagtttg tggcatctga tcaatatgct gtaacgaatg ttctaaatac ataactggtg
     1861 caaatcgaaa atttcctttt gcaatattga ccgatcggat ttttccggca aaatcgtaaa
     1921 tatccgaaaa tagaaattca tgaatttcag ataaaccttt aaatgtacca acttcgattt
     1981 catttatttt tccacaatcg tacaattctt tcgctttttg tttactgagt ttttcttcaa
     2041 ccttagccaa ctcaacttga ttggttaatc ccaatttatt ctctaaaatc atgcgaaatc
     2101 tccttaactt tatgacataa gtttatgaaa cactctcaac cccattatat cgcgattttg
     2161 tttatgtgtc aaaaactatt aagtttctga cccccgatag atttttcaag tgtctctatt
     2221 ttgcacagtc ta
//
LOCUS       DACDXO010000142         2104 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E23605_2017
            SAMN12639989-rid9881463.denovo.152, whole genome shotgun sequence.
ACCESSION   DACDXO010000142 DACDXO010000000
VERSION     DACDXO010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639989
            Sequence Read Archive: SRR10023389
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2104)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2104)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741914.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:42:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,972
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 33 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 17 of 73
            Pseudo Genes (multiple problems)  :: 10 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2104
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639989-rid9881463.denovo.152"
                     /isolate="E23605_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            169..1539
                     /locus_tag="IWM35_002784"
     CDS             169..1539
                     /locus_tag="IWM35_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4357 domain-containing protein"
                     /protein_id="HAP9920568.1"
                     /translation="MMDKSNEEQTTLYTKYWRRLEEVFDNSKGMEDFFRYYLAAITGE
                     YSAKHILYQAFKDYWHKERDVSSDAELLQKLVRYAGYFARLYYNKPEGKYSEVLSDFQ
                     SMESMMPAPFVLGLSEWYYHDQFITEDQYIDAIKVVNDYQLRRYFNGDDTSRVSKAFP
                     SYLKSVRKYAKANGYENIVDIVIYVLVTRNQSNNMALPSDKALKSNLLNANAYAMRLA
                     RWLLEKIENRENSATLDMSNLSIEHIMPQTSTSYWEEKAGTSGEEYTGLVNTIGNLTL
                     VTNPDNSAAGNKDFETKKKIFEDTLHIRMNKDLYELTEWTSSDISARSEALINELITM
                     YPYLRSSGDYEHDGNREIFLEAQGIKATGYLNEDETVIIHSGSEIYSKIKDIASDSLD
                     ETRQELLDNGIIEETIGGLQFVQDYTASSVSNAAALLLGGSRNGWDYWKYDNGISIND
                     SLRNKK"
     gene            1560..1844
                     /locus_tag="IWM35_002785"
     CDS             1560..1844
                     /locus_tag="IWM35_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9920569.1"
                     /translation="MDKNKLRYAILKLRDEKKNPFIELKGQVPEEDILEQTKFLSRNG
                     LLESMVWADNTVHIWGSVSLEGEKYLVENSGLAKAYALAKEVKNWIPFWG"
BASE COUNT          731 a          308 c          444 g          621 t
ORIGIN      
        1 ttgatttacc aacatgttta gacattttcc tctaggaaaa aattaactag caccacgcgt
       61 attgtttata ttaaattaga tgaccgtgat aatgcacagc aaatttttga aagtatcaat
      121 tctaccggtg aaagattgac tgcctcggat ttgattcgaa acttcatcat gatggataaa
      181 tctaatgaag agcagactac gctttatact aaatattggc gtaggttgga agaagtcttt
      241 gataattcta aaggaatgga agactttttc agatactacc ttgctgcaat aactggggag
      301 tattcagcaa agcatattct ttatcaagca ttcaaagact attggcataa agagcgcgat
      361 gtatcaagtg atgccgaatt gctccaaaaa ttagtgcgat atgctggtta ttttgcgaga
      421 ctttactata acaaacctga aggaaaatac tcagaagtat tgagcgattt ccaaagtatg
      481 gaaagtatga tgcctgcacc ttttgttttg ggattatctg aatggtacta tcatgaccaa
      541 tttatcactg aagaccaata tattgatgcg attaaggtag tgaatgatta tcagcttagg
      601 agatatttta atggggatga tacaagcaga gtgtcaaaag catttccttc atatttgaaa
      661 agtgttagga agtacgctaa agctaatgga tatgaaaata tcgttgatat tgtaatttat
      721 gttcttgtta ctagaaatca atcaaataat atggcacttc catctgacaa ggcattgaaa
      781 tctaatctat tgaatgccaa tgcttatgcg atgagattgg cccgttggtt acttgaaaaa
      841 atagagaatc gagaaaattc agcaacactt gatatgagta acttgtccat tgaacacatc
      901 atgccacaga ctagtacttc atattgggaa gagaaggctg gtacatcggg ggaagaatac
      961 acaggattag taaataccat tggtaattta acactcgtta caaatcctga taattcggcc
     1021 gcgggaaata aagactttga gaccaaaaag aaaatctttg aagataccct tcatattcgt
     1081 atgaataaag atttgtacga attgactgag tggacatcat cggatatttc tgccagaagt
     1141 gaagctctaa tcaatgaact gattactatg tatccctatt tgagatcgtc gggcgactat
     1201 gaacacgatg ggaataggga aattttctta gaggcgcaag gaataaaagc aactgggtat
     1261 ttgaacgaag acgagaccgt gattatacat tcaggttcag aaatttattc taagattaaa
     1321 gatattgcat ctgattcgct agatgaaact cgccaagagc tgttggataa tggaatcatt
     1381 gaagaaacaa ttgggggact acaatttgtt caagattaca ctgcctcatc tgtttcgaat
     1441 gcagcagctc tgctattagg gggtagccgg aatggttggg actactggaa atatgataac
     1501 ggaataagta ttaatgattc gctaagaaat aagaaataga gcatagaggt ggtgttaggt
     1561 tggataagaa caaactaaga tatgcaattc ttaaattgcg tgatgaaaaa aagaatcctt
     1621 ttattgagct taaagggcaa gttccagaag aagatatttt agaacaaact aaatttcttt
     1681 cgcgcaatgg cttactagaa tccatggttt gggcggataa cactgttcat atctggggga
     1741 gtgtttctct ggaaggggaa aaatatttag ttgaaaactc aggtcttgct aaggcgtatg
     1801 cattggcaaa agaagttaaa aattggattc cattttgggg ctaagataag tttttaaacg
     1861 gagaaggtag caaatcataa ttggtttgtt gccttttttg attgcccaaa aagaaaaatg
     1921 acaaagtata gagataaatg acatagtaca cgggaaaaat gacattgtat caccgacaac
     1981 gtctaaatag atgttgatat atatacgttc cgtacaaata attataaaaa tttaaatgat
     2041 ttgagtagaa gtatcagtga atatctcaag gggaatagct atctcaagat tcgattttca
     2101 cata
//
LOCUS       DACDXO010000143         2012 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E23605_2017
            SAMN12639989-rid9881463.denovo.153, whole genome shotgun sequence.
ACCESSION   DACDXO010000143 DACDXO010000000
VERSION     DACDXO010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639989
            Sequence Read Archive: SRR10023389
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2012)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2012)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741914.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:42:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,972
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 33 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 17 of 73
            Pseudo Genes (multiple problems)  :: 10 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2012
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639989-rid9881463.denovo.153"
                     /isolate="E23605_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            628..>2012
                     /locus_tag="IWM35_002786"
     CDS             628..>2012
                     /locus_tag="IWM35_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAP9920570.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVGKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPAILGQKQGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFT"
BASE COUNT          616 a          364 c          408 g          624 t
ORIGIN      
        1 tatcaagagg tcttgagcct gccgtttaac tctgagccac aaggagcaat ttcaaaaaat
       61 agctccttat atttttcgtt tggttgggct tattaccgca gatgcaagct tttgaacacc
      121 gtttaccaag aggttgtaaa attagcgtcc tgacataatc taataccgag ggtcagctga
      181 ttgaacaccg tgtatcaaga ggtcttgagc ctgccgttta actctgagcc acaaggaaca
      241 atttcaaaaa acagcttctc atgttttata aatattgtga cttgtggccg aggagttggc
      301 aggagaacac cgtttaagga agaagttgtg aaaaagctgt cctgcatcaa gaaataagga
      361 gctgcgacaa gaattttgtc acagcctctt tttttgtacg aataccgaga aactggcttt
      421 tgaataccgt ggagtgaagc ggtcaccaca agacttttat ctcttcatta gtttaatcgc
      481 ttacatcgca gcggttttat gctgtgaaaa gtggacggaa taatctttag aaagaataaa
      541 aaaatcttaa gataacaact tttttaggaa attttcattt ttcaggagca aaataagaaa
      601 aaatgtgaat agagagaagt gatatacgtg acacaactta aaaaattgtg ccaaaatcgt
      661 ttagcaatcg ttttgacagc tgttttcttt ttctggctga agacgatcat tgcctactat
      721 gcagattttt cattaggtgt ggaaggtacg atccagtact ttatcttgtg gatcaaccca
      781 attgcgacta ccttattgtt ttttggcctt tctttgtata ttaaaaagcc aaagccagcg
      841 ttagctattc ttttgatcat tgatatttta aatacgcttt tgttgtactt gaatattatt
      901 ttttatcggg aattcacaga tttcattaca gtcaaatctg tattaggctt ttcgaaagtg
      961 tcgcaaggat tgtcaggaag ctccttttct ttgatgaagc cccatgatgt catctactgg
     1021 ctggatatcg ctgtgttcat tgggctactg gtttggttga aggtcaagaa aatacctatc
     1081 aaaagcaacc ctgtcggcaa accgatggct atcgctgtta cctgtctttc agggcttatt
     1141 ttttcaggaa atctagcgct gagcgaagcc aaccgtccac aattgctgca gcgaacattt
     1201 gaccgttcat atattgtcaa atatctagga atcgatgcgt atactattta tgacggaatc
     1261 aaaacaggaa tgactagcag tgttcgcgcg catgcaagca gtaatgggat tgatgaagtt
     1321 ttagattata caaagaaaca ttatgcagag ccaaatcccg aaacatttgg gatcgctaaa
     1381 gggaaaaatg tgatcgttct ccatttagaa agtttccagc agtttttgat taatatgaag
     1441 gtagacggac aagaagtcac accgttttta aatagtattt tccagaatca agcaacgatc
     1501 agctttgaca atttctttca tgaagtagga cagggaaaga caagtgatgc tgaaaatatg
     1561 ttggaaactg gcacatttgg tttacctcaa ggctcattat tcacagaact tgggtcagac
     1621 aatgtctttc aagccgcacc agcgatcctt ggacaaaagc aaggctatac aagcgctgtt
     1681 ttccatggga atgtagctag tttttggaat agagatcatg tatataaaaa cttaggttat
     1741 gacaatttct ttgatcgatc ttattttgat gaatccgacg aaacgttagg ttatgggata
     1801 ttagataaag atttattcag agaatctgcc caatatcttg agcatctaca acagccgttt
     1861 tacacgaaat ttctttccgt aaccaaccac acgccttatt atacggatga caagaatttc
     1921 gactttccat ctttgaatac tggcaatagc acagtagata attatgttcg aacggcacat
     1981 tatctggatc aatcattgga acaatttttt ac
//
LOCUS       DACDXO010000144         2003 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E23605_2017
            SAMN12639989-rid9881463.denovo.154, whole genome shotgun sequence.
ACCESSION   DACDXO010000144 DACDXO010000000
VERSION     DACDXO010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639989
            Sequence Read Archive: SRR10023389
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741914.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:42:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,972
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 33 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 17 of 73
            Pseudo Genes (multiple problems)  :: 10 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2003
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639989-rid9881463.denovo.154"
                     /isolate="E23605_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..378
                     /locus_tag="IWM35_002787"
     CDS             <1..378
                     /locus_tag="IWM35_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HAP9920571.1"
                     /translation="KNVSTEDFFNYCRLEESGVTVTSGTDLPLFITDIPESIIRAVFR
                     RFPEGEEVWEKETSFTTTELLKSFTSNSFKVNGLSEYGEIKVGKSASLAVFSEDLCTD
                     DWQKVMAAEVIATYLDGDLVYKK"
     gene            426..1826
                     /gene="yfcC"
                     /locus_tag="IWM35_002788"
     CDS             426..1826
                     /gene="yfcC"
                     /locus_tag="IWM35_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HAP9920572.1"
                     /translation="METMKRKFTFPHSYTLIFILIILAAILTWIIPSGQFETVVENVA
                     GMEKTTVVPGTYHQIDKADYGQGFSSILMAPAEGIISAVEVVAFVLIVGGAFGIILKT
                     GAIDRGLFTLAKVLGDKGILVIPMSMILFSLGGSTFGMSEEVIPLFAVFISLMFSLGY
                     DSMTAILILFLSTQAGYVGSTINPFNVLIAQGVAGVYGNPLLWFRMLSWIVITGISIV
                     FTMLYASRVKKNPLSSIVYESDLKVKHKFQNFDTEKEFSTQDKLIIAGFIVGLGIMIW
                     GIISKGWYMVEISALFLGLGLFAGGIARFSQKEIAEAFVEGCSDFAYAAVIIGLARGV
                     LVILEGGMIIDTILNSLANLLGGLPKFLFTTILLISQVIVSFFVPSSSGAAALTMPVM
                     APLGDLMNVSRDVVVLSNQFGNGLMNIISPTSGVLLAGLAIAEINFSKWFKVGFKIFA
                     ILTLVSAVLLYIATLF"
     gene            1859..>2003
                     /locus_tag="IWM35_002789"
     CDS             1859..>2003
                     /locus_tag="IWM35_002789"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAP9920573.1"
                     /translation="MIKINQLAGMNIHYLFYSLKEFFAYQQKIGISSVELWGGAPHFF
                     IDAF"
BASE COUNT          555 a          339 c          432 g          677 t
ORIGIN      
        1 aaaaatgttt ccacagagga cttctttaat tattgtcgat tagaggaaag tggtgtaaca
       61 gtcactagcg gtacagattt accgctgttt attactgata ttcctgaatc gattataaga
      121 gcagtattcc gtcgttttcc tgaaggagaa gaagtctggg agaaagaaac aagcttcacg
      181 acaaccgagc tgctgaaaag ttttacaagc aacagcttca aagtaaatgg tttatcggag
      241 tacggagaaa taaaagtagg gaaaagcgcc agcttagctg ttttcagcga ggacctctgt
      301 actgatgact ggcaaaaagt gatggctgcg gaagttatcg ctacctatct tgatggtgac
      361 ttagtttaca agaaataatg cttttcgata taacgttata aaacgaatta aagagaggtt
      421 aaaaaatgga aacaatgaaa agaaaattca ccttcccaca tagttataca ttgattttta
      481 tcttaattat tttggctgca attttaactt ggataattcc tagtggtcag tttgagacag
      541 ttgttgaaaa cgtcgcaggt atggaaaaaa caacggttgt tccgggaacc tatcaccaaa
      601 tcgataaggc agattatgga caaggttttt cctctatact aatggcacca gctgaaggga
      661 ttatttcagc tgttgaggtt gttgcttttg ttctcatcgt tggtggtgcc ttcgggatta
      721 tcctaaaaac gggagccatc gatcgagggc tgtttacctt agcaaaagtt ctgggagaca
      781 aggggatctt agttattccg atgtcgatga ttctattcag cttaggagga tcaacctttg
      841 gcatgagcga ggaagtcatt cccttatttg cggtgtttat ttctctgatg ttttctcttg
      901 gctatgattc catgactgcg atcttgattc tatttttaag tacacaagcg ggatatgttg
      961 ggtcgacgat taatcccttc aacgttttaa tcgcacaggg agtagctggc gtttacggaa
     1021 atcccctgct ttggtttcgg atgctatcct ggattgtcat tactgggatt tcaattgttt
     1081 tcaccatgct ttatgctagc agagtcaaaa agaatccact gtcctctatt gtttatgaga
     1141 gtgatttaaa ggttaagcat aagtttcaaa attttgacac tgaaaaagag ttttcaacac
     1201 aagataaact aattatcgcc ggttttatcg taggtttagg aatcatgatt tggggtatca
     1261 tttccaaggg atggtatatg gttgaaattt ctgcactatt cttaggatta ggtctttttg
     1321 ctgggggcat cgctagattt tctcaaaagg aaattgctga agcttttgtc gaaggctgtt
     1381 cagatttcgc ctatgctgct gtaatcattg ggctagctag aggagtcttg gtaatcttgg
     1441 aaggtggaat gattattgac actattctaa acagcttggc taacctttta ggtggtcttc
     1501 caaaattcct gttcactacg attctactca tttctcaagt aattgtttcc ttttttgttc
     1561 catcctcctc aggagctgcg gcattaacga tgccggttat ggcaccattg ggtgatctaa
     1621 tgaatgtaag tcgtgatgtt gttgttctaa gtaaccagtt tggaaatggt ttaatgaata
     1681 ttatctcccc aactagtggt gtattactgg caggattagc catcgcagag attaatttct
     1741 ctaaatggtt taaggttggc ttcaaaatat ttgccatctt aacccttgtt tctgctgttt
     1801 tgttatacat tgctacactc ttttagtctt aatcatccat taataggagg caatagttat
     1861 gattaaaata aatcaattag caggaatgaa tatccactat ttattttatt ctctcaaaga
     1921 atttttcgct tatcaacaga aaattggtat ttcttctgtt gaactttggg gaggtgctcc
     1981 gcattttttc atagatgctt tta
//
LOCUS       DACDXO010000145         1994 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E23605_2017
            SAMN12639989-rid9881463.denovo.155, whole genome shotgun sequence.
ACCESSION   DACDXO010000145 DACDXO010000000
VERSION     DACDXO010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639989
            Sequence Read Archive: SRR10023389
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741914.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:42:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,972
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 33 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 17 of 73
            Pseudo Genes (multiple problems)  :: 10 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1994
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639989-rid9881463.denovo.155"
                     /isolate="E23605_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            274..840
                     /gene="amaP"
                     /locus_tag="IWM35_002790"
     CDS             274..840
                     /gene="amaP"
                     /locus_tag="IWM35_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HAP9920574.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
     gene            853..1041
                     /locus_tag="IWM35_002791"
     CDS             853..1041
                     /locus_tag="IWM35_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HAP9920575.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            1054..1611
                     /locus_tag="IWM35_002792"
     CDS             1054..1611
                     /locus_tag="IWM35_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAP9920576.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            1643..1885
                     /locus_tag="IWM35_002793"
     CDS             1643..1885
                     /locus_tag="IWM35_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAP9920577.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
BASE COUNT          725 a          308 c          406 g          555 t
ORIGIN      
        1 aaataaatag aatcttgtaa taataactga gcataatctt tgcaattttt aatcttgaat
       61 aatatccttt aagtatagcc agcaatcaaa agtaaataga gaataagtaa gcaattgtta
      121 tttcagtatc agactggcaa taaaacgccg ttatgatcca gcaatgtttg gtagcgcttt
      181 atttactgat gctatgctaa ctgttcgcta tctatactgt gattgtcggt agagttattt
      241 acagggtaaa aataagttta taggaggtgg aaaatgaata aggggttaaa aataatcggt
      301 gttatcatga gtttactgct actttcagta tttctagcga ttgcgttgat caatgcgccg
      361 tatattatgc ctgaacaatt ggaacgattt cgcttcttta ctttaacgaa ttactacatg
      421 caacaatata ttttctgggc agctgtagta tttgccgttc ttgctattat catcttgcta
      481 gtcgttcttt tctatccaaa gtcaagagga actttcgtta tgaaacggga agatggcaaa
      541 ctaacaatcg acaaaaaagc gatcgaaggg ttggtacgtt cccatttaca cgaagaagag
      601 ttcattcatt cacctaaagt acggatacgt tcaacgaaaa atcgaatcca tatccatgtc
      661 aacggagact tgaaacgaac ttcttcatta gtagggaaaa caggtgtatt gatgcaagat
      721 atcgaagaag aagtaactaa agtactcgga acaaaagaaa cggtcaaagt ggcagtcaca
      781 tacagcggtt atgaagaaca agaagataca agagactaca agcactctcg agtggaatag
      841 gagggccaaa taatgaaaga actattttca gcatacaagc tccctattat ctttgggggt
      901 cttggactac tgctagctgt tttatggtta acgattggat ttttcaagac attgttgatc
      961 ataatcatga cagcaattgg cgcaggaatc ggtttttatt tgaaacagtc aggaatagtt
     1021 gatcaatttt ttaaatcata ggaggaaaca aagatggaaa acaaaaacat gaatacaaca
     1081 aatggatcag ttttggcaaa tgaaaaagga gaaaaccgca tgatgaatgg tgaattgact
     1141 tatgaagaca aagtcatcca aaaaatcatc ggttatgcat tgaaagatgt agatggtcta
     1201 ttgacattag atggcggatt tttctcaaat atggctgaaa aattagtcaa cacagataac
     1261 gtaacagcag gtatcaatac agaagtaggt aagaaacaag tagctgtgga tctagatgtg
     1321 atcgtagaat acggtaaaga catcgaagat atttacaacc aaatcaaaga attgatcagt
     1381 actgaagtaa acaatatgac acatttagat gttatcgaag tgaatgtgaa tgttgcagac
     1441 atcaaaacac aagaagaata ccaaaaagat caagaaacag tacaagacaa agtaacagaa
     1501 gcagcaaaat ccacaggaca atttgcttca cgccaaacag ataaagcaaa atctgctgta
     1561 ggcaaaggtg cacaaaaagt caaagaaaat aacgaaccac gtgtacaata agaagtaaga
     1621 agtcagttag gaggaattta aaatgggatt tatttgggca ttaatcgtcg gcggtatcat
     1681 tggagcaatt gcaggagcaa tcacgaataa aggatcatca atggggatca tcgcaaacat
     1741 cattgctggt ttagtaggtt cagcaattgg acaagcttta ctaggaactt ggggaccaac
     1801 aattgcagga atggctatca ttccatcatt gatcggtgca atcattttag tattagtcgt
     1861 ttcattcttt gcacgtaaat tataagcaaa ttgaagaaaa acttatgttg aaatgaagaa
     1921 caaatttgag aaccgtagcg acactcgctg cggttctttt ttatatattt tatatgaagt
     1981 attctttatt cctt
//
LOCUS       DACDXO010000146         1796 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E23605_2017
            SAMN12639989-rid9881463.denovo.156, whole genome shotgun sequence.
ACCESSION   DACDXO010000146 DACDXO010000000
VERSION     DACDXO010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639989
            Sequence Read Archive: SRR10023389
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741914.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:42:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,972
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 33 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 17 of 73
            Pseudo Genes (multiple problems)  :: 10 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1796
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639989-rid9881463.denovo.156"
                     /isolate="E23605_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            29..1381
                     /gene="brnQ"
                     /locus_tag="IWM35_002794"
     CDS             29..1381
                     /gene="brnQ"
                     /locus_tag="IWM35_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HAP9920578.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
BASE COUNT          503 a          309 c          353 g          631 t
ORIGIN      
        1 ttttaaaaaa accgtaaagg agttatgtat gacaaaaaaa ttatcattta aagactatct
       61 ttttataggc tccatgcttt tcggtctatt ttttggtgct ggaaatctta tctttcccgt
      121 tcatttgggt caagaagcag gcgctgctac cttttgggcg aatctaggtt tcttggttac
      181 cggtatcggt cttcccttct taggggtgat tgccattggt gtatcgaaaa gctcaggtgt
      241 atatgaatta gcaaaacgga taaatcaaac atatgcgatg gtttttacca tcttgcttta
      301 cttagttatc ggaccgtttt ttgctttacc tcgccttgca accacatctt tcgagattgg
      361 tttagcacca tttatcgatc ctagcagaca gacgctatat ctagcaattt tcagtattgt
      421 attttttgtg ctggcttggt ggttttcaag aaaaccgaca aagatattag attatgttgg
      481 aaagttcctg aatcctgcat ttttggtttt attaggtgct ttgcttctcc ttgctttttt
      541 gaatccacta ggttctgtaa atcaagcgcc tattcagcca aattatcaag agcatgcttt
      601 tttcacaggg tttactcaag gatataacac attagatgcg cttgctgctt tagcatttgg
      661 gatcatcatt gtcacaacaa tccaaaacat gggtgtaaca aaacctgctg aaatcgctaa
      721 agacacgatc aaatctggtg cgatcagcat tatattgatg ggaatcattt atactttgct
      781 cgcttattta ggtgctatga gcttaggtag ctttgctcta agtgaaaatg gcgggatcac
      841 gctggctcag attgctgatc attatttagg tacttatggc agtatcttat tagcatttat
      901 cgttatatta gcttgcttga agaccggtat tggtttgatt acagcttttg cagaaacatt
      961 cacagatttg tttccgcaaa gaaattacgc attctttgtt gcacttgcta gtatccttcc
     1021 ttgtttagcg gcaaacgtag gcttgacgaa tatcattcaa ttttcgttgc ctgttttgat
     1081 gttcatctat ccattagcaa tgacactgat tttactagtg ttagttggtc ctttgtttaa
     1141 gcagcgtacg gctgtttatc gaatgactac ttattttaca ttgattgctt cgatttttga
     1201 tgggttgaac gcttgcccag aaactatcaa acaaacacct attgttcaaa atcttttata
     1261 tgttgcggaa agctacttgc cgtttttcaa attggggatg gggtggatcg taccagcagt
     1321 tatcggtttt gttatcggac tgatttggag tttcgctaag aaagaagaag tagcagattg
     1381 ataaaagagg ttgtgaaaga gacgttcagc cttgagcaat aaaaagtaat tttgaaaaat
     1441 ggcttctcac atttttgcat aaatttgact taataccgag aaactgactt ttgaacaccg
     1501 tttattgaga ggtcgtgaaa aagccgctta gtttcaagta ttaagaaata ttttgtgaaa
     1561 atggcttctc acatttttgc ataaatttga cttaataccg agaaactggc ttttgaacac
     1621 cgtttattga gcgtctataa actgacctga gtattagaga aacaaaacgg caaagtagct
     1681 ccatatactt tgccgttttg ttttgaacta ataccgaaga gaagctgatc aaacaacatt
     1741 tcttttataa tatagatttt agacatatat atgatataaa aatagttttc gcaaaa
//
LOCUS       DACDXO010000147         1642 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E23605_2017
            SAMN12639989-rid9881463.denovo.157, whole genome shotgun sequence.
ACCESSION   DACDXO010000147 DACDXO010000000
VERSION     DACDXO010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639989
            Sequence Read Archive: SRR10023389
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741914.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:42:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,972
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 33 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 17 of 73
            Pseudo Genes (multiple problems)  :: 10 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1642
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639989-rid9881463.denovo.157"
                     /isolate="E23605_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(23..107)
                     /locus_tag="IWM35_002795"
     tRNA            complement(23..107)
                     /locus_tag="IWM35_002795"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(71..73),aa:Leu,seq:caa)
     gene            complement(125..196)
                     /locus_tag="IWM35_002796"
     tRNA            complement(125..196)
                     /locus_tag="IWM35_002796"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(162..164),aa:Gln,seq:ttg)
     gene            complement(206..278)
                     /locus_tag="IWM35_002797"
     tRNA            complement(206..278)
                     /locus_tag="IWM35_002797"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(243..245),aa:His,seq:gtg)
     gene            complement(340..424)
                     /locus_tag="IWM35_002798"
     tRNA            complement(340..424)
                     /locus_tag="IWM35_002798"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(388..390),aa:Leu,seq:caa)
     gene            complement(445..515)
                     /locus_tag="IWM35_002799"
     tRNA            complement(445..515)
                     /locus_tag="IWM35_002799"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(481..483),aa:Cys,seq:gca)
     gene            complement(561..632)
                     /locus_tag="IWM35_002800"
     tRNA            complement(561..632)
                     /locus_tag="IWM35_002800"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(598..600),aa:Gln,seq:ttg)
     gene            complement(644..716)
                     /locus_tag="IWM35_002801"
     tRNA            complement(644..716)
                     /locus_tag="IWM35_002801"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(681..683),aa:His,seq:gtg)
     gene            complement(719..792)
                     /locus_tag="IWM35_002802"
     tRNA            complement(719..792)
                     /locus_tag="IWM35_002802"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(758..760),aa:Trp,seq:cca)
     gene            complement(799..879)
                     /locus_tag="IWM35_002803"
     tRNA            complement(799..879)
                     /locus_tag="IWM35_002803"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(843..845),aa:Tyr,seq:gta)
     gene            complement(881..956)
                     /locus_tag="IWM35_002804"
     tRNA            complement(881..956)
                     /locus_tag="IWM35_002804"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(921..923),aa:Phe,seq:gaa)
     gene            complement(992..1064)
                     /locus_tag="IWM35_002805"
     tRNA            complement(992..1064)
                     /locus_tag="IWM35_002805"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1029..1031),aa:Asp,seq:gtc)
     gene            complement(1074..1146)
                     /locus_tag="IWM35_002806"
     tRNA            complement(1074..1146)
                     /locus_tag="IWM35_002806"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1111..1113),aa:Val,seq:tac)
     gene            complement(1162..1233)
                     /locus_tag="IWM35_002807"
     tRNA            complement(1162..1233)
                     /locus_tag="IWM35_002807"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1198..1200),aa:Glu,seq:ttc)
     gene            complement(1248..1337)
                     /locus_tag="IWM35_002808"
     tRNA            complement(1248..1337)
                     /locus_tag="IWM35_002808"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1301..1303),aa:Ser,seq:gga)
     gene            complement(1377..1450)
                     /locus_tag="IWM35_002809"
     tRNA            complement(1377..1450)
                     /locus_tag="IWM35_002809"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1414..1416),aa:Asn,seq:gtt)
     gene            complement(1460..1575)
                     /gene="rrf"
                     /locus_tag="IWM35_002810"
     rRNA            complement(1460..1575)
                     /gene="rrf"
                     /locus_tag="IWM35_002810"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT          457 a          430 c          372 g          383 t
ORIGIN      
        1 aaagttgcta tattgataat tataccggcg gccggggtcg aaccggcacg tcctcaacgg
       61 acactggatt ttgagtccag cgcgtctgcc aattccgcca cgccggcaaa tttaaaaata
      121 ataactgggg tagctggatt cgaaccaacg catgagggag tcaaagtccc ttgccttacc
      181 gcttggctat accccaagaa taaagggcga atgatgggaa tcgaacccac gaatgccgga
      241 gccacaatcc ggtgcgttaa ccacttcgcc acattcgcca tcataaaaga tgttatttaa
      301 tttctcaata aaaaactaag atgaagacat cttagtcaat accggcggcc ggggtcgaac
      361 cggcacgccc tcaacgggca ctggattttg agtccagcgc gtctgccaat tccgccacgc
      421 cggcaagttg atttattagt actaaggcgg taaccggatt tgaaccggtg atgaaggttt
      481 tgcagacctc tgccttacca cttggctata ccgccattat actattatca atctataaaa
      541 taaagaataa attatagaaa ctggggtagc tggattcgaa ccaacgcatg agggagtcaa
      601 agtcccttgc cttaccgctt ggctataccc caagaataaa aagggcgaat gatgggaatc
      661 gaacccacga atgccggagc cacaatccgg tgcgttaacc acttcgccac attcgccatg
      721 gcaggggcag caggaattga acccacacta acggttttgg agaccgctgt tctaccttta
      781 aactatgccc ctataaaatg gaggagagtg gattcgaacc actgaaccct aaggaacgga
      841 tttacagtcc gtcgcgttta gccacttcgc tactcctcca tggtggcgcg ggacagaatc
      901 gaactgccga cacacggagc ttcaatccgt tgctctacca actgagctac cgcgccatat
      961 tttaaaatta ttaattgaat aacaagaacg acggttccga cgggaatcga acccgcgatc
     1021 tcctgcgtga caggcaggca tgttaacccc tacaccacgg aaccagttca ttatggaggt
     1081 taacgggatc gaaccgctga ccctctgctt gtaaggcaga tgctctccca gctgagctaa
     1141 acctccaaca ttcaattaaa atgacccgta cgggattcga acccgtgtta ccgccgtgaa
     1201 agggcggtgt cttaaccgct tgaccaacgg gccaggtaaa tttgctacgg agagtaaggg
     1261 attcgaaccc ttgagacagt ttggcaccat ctacatgatt tccaatcatg ctccttcggc
     1321 ctctcggaca actctccaga gtgaaagaag ctcaatactc tttctcaaaa tgagaactcc
     1381 ggcagtagga ctcgaaccta cgacatcatg attaacagtc atgcgctact accaactgag
     1441 ctatgccgga ataacatcag cgtggcgacg tcctactctc acaaggggca acccctcact
     1501 acaatcggcg ctaagaagct taacttctgt gttcggcatg gttacaggtg tatccttctc
     1561 gctatcgcca ccacactgtg gtgttatctt ttattgagta aattttgttc actcaaaact
     1621 ggatttgaag tcacataagt tt
//
LOCUS       DACDXO010000148         1553 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E23605_2017
            SAMN12639989-rid9881463.denovo.158, whole genome shotgun sequence.
ACCESSION   DACDXO010000148 DACDXO010000000
VERSION     DACDXO010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639989
            Sequence Read Archive: SRR10023389
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741914.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:42:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,972
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 33 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 17 of 73
            Pseudo Genes (multiple problems)  :: 10 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1553
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639989-rid9881463.denovo.158"
                     /isolate="E23605_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            191..445
                     /locus_tag="IWM35_002811"
     CDS             191..445
                     /locus_tag="IWM35_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAP9920579.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDIIQAKQEDNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            446..763
                     /locus_tag="IWM35_002812"
     CDS             446..763
                     /locus_tag="IWM35_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAP9920580.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
     gene            complement(1397..>1553)
                     /locus_tag="IWM35_002813"
     CDS             complement(1397..>1553)
                     /locus_tag="IWM35_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HAP9920581.1"
                     /translation="SSLEKLGCQNFNSRSVKGLESRFESILLAYSVLLSRAQRRFEGT
                     LRYSLGY"
BASE COUNT          552 a          231 c          293 g          477 t
ORIGIN      
        1 gatgttaacg gaagtgtatg gtgagtagtt cactcgtatt ttgatatcgt ttgtatatgt
       61 tttcagcgtt ctaagtggct tattttgtcc aataataata tggagtggaa tcccttaaga
      121 aacaccgttg gcaattgttg ttataacatg ttataattta ttcatcaaaa actaaaagga
      181 gtgtgataac atgggaaaag aactaaaggt tagaaagatc ggcaatagcg tgggagtgat
      241 ccttccaagc tctcttggat taaaaagcgg agacattatc caagccaaac aagaagacaa
      301 cttattcata ttagatacta ctcaaatagc aaaagaacac gatcgcaaat tgattgaaga
      361 aagtttccaa gactttgaga aaggtttaac agtttcagaa attgaaatgg taaaagcttt
      421 cggaaaatac ggctggagtg aatagatgaa agagtacacg ctagtttatt ctaaaagttt
      481 tcagaccagc cttgaaacga taatagaaga atgggaaaat gagcttgctc tttctccagc
      541 cagcataaaa agatttgtag gtgcgatcca gaaggcttta gaactaacca aaaattttcc
      601 aactatgcat gaggaagttt ccgaaatcta tggaatggat atcccaacat atcggatcgt
      661 gatcggcaaa agctatgcct tattttatcg gatcgaccat gaaaaaaaag agatacttgt
      721 aggacgtatt ttccaatcta aacaaatgaa actaggcttt taacaataat taggaaaaag
      781 tcgctcatcc tctacaggtg ggtgattaat gccgctcgat atggttgtta accgtcggtt
      841 actcgaagat tgatatataa gacttagtac ctttttattt tgctccatgt tatggccgaa
      901 ggtctaaaga tacaatcatt agtctagaga aaaattaaga accttaggaa ctacggggat
      961 cgcttggtga ataagagaca ctgctattag tgaaaaaata agatatcaag tacgctctat
     1021 tcccaaaaat ttcgtgactt tactcatgag aggttcaagt agtatatttt taatcaaaat
     1081 tgacgtaact tttaataaat actacctaaa taaggtagta tttatttttg tgttttaaaa
     1141 gaggaagtcc aatatatcta tgggaaactt cttaaatatt atgttggtac atgaaaatat
     1201 acttccctgc tagactattt acaacatttt actttgtacg tattcttgtt ttaaaaggaa
     1261 tgggataaaa tataggtaca ataactattt gatgctaaat ataattaatg gaggagagat
     1321 agtgaacata cttaatgaaa aaagatatgt atggtttgct ggtaaaaaat atattaaccc
     1381 gtggtgctag ttaattttaa tatcctaaag aatatctcaa agttccttca aaacgtcgtt
     1441 gtgctcgact taatagaaca ctgtaagcca gtaatatgct ttcaaatcta ctctctagcc
     1501 ctttaacaga acgtgagttg aaattttgac aacctaattt ttctaaggaa gaa
//
LOCUS       DACDXO010000149         1509 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E23605_2017
            SAMN12639989-rid9881463.denovo.159, whole genome shotgun sequence.
ACCESSION   DACDXO010000149 DACDXO010000000
VERSION     DACDXO010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639989
            Sequence Read Archive: SRR10023389
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741914.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:42:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,972
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 33 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 17 of 73
            Pseudo Genes (multiple problems)  :: 10 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1509
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639989-rid9881463.denovo.159"
                     /isolate="E23605_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            101..868
                     /locus_tag="IWM35_002814"
     CDS             101..868
                     /locus_tag="IWM35_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAP9920582.1"
                     /translation="MKKDELTKYNEEFLATIDMKMDLKSYELYQLLFSYISPTKLKKT
                     GVVDLPVKAIINYMDVDQNLETELKTIVNTLQRQAVYEISNLDKKYKTYTTVSAIDRI
                     HWDLEEHSVSFSFSEDVIPLIVSIKKKYPFFVFEDLASFQKKYSIVLYKYLAMYYKKY
                     LIAKNKGDFEKAEIYKNPLIEIVKIREITKTENEYKTISNFDRRVLRESVEEINEKSM
                     LNIDFAKVRKGRNYNFAVQFNISSEFDEKTYREKKLG"
     gene            1060..1458
                     /locus_tag="IWM35_002815"
     CDS             1060..1458
                     /locus_tag="IWM35_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin HicB"
                     /protein_id="HAP9920583.1"
                     /translation="MKYFFCAVFEWKGNEYAVKFPDFPDLYTHGETLEDAFEMAQEVL
                     EGYLVLLELQEKEIPSPTPVQELNARIKRSENVNLFVVKANTKVAKARNKQNYDRKSV
                     TVEHYLNVLAAEENINLSKALNDLLREKLI"
BASE COUNT          561 a          189 c          282 g          477 t
ORIGIN      
        1 aatactgtta aatatctcga aaaatcaatt acgagttatt tgccttatgt gaacctttat
       61 gattaacaac tgaaaaaatg cgtgaaggaa ggaaaaactt ttgaaaaaag atgaactgac
      121 aaagtacaat gaggagttct tagcaacaat tgacatgaaa atggatttaa agtcgtatga
      181 actttatcaa ttgctgttca gttatatctc acccacaaag ctaaagaaaa caggcgtagt
      241 agatttacct gttaaagcga ttattaatta catggatgtt gatcaaaatc ttgaaacgga
      301 actaaaaacc atagttaaca ctttgcaaag acaagcagtt tatgaaattt cgaatctaga
      361 caaaaaatat aagacgtata ctactgtatc tgctattgat cggattcatt gggatttaga
      421 agagcatagt gtatctttca gtttttcaga agatgtaatt cccttaattg tctcaatcaa
      481 aaagaagtat ccgttctttg tttttgaaga cttagcatct tttcaaaaaa aatatagcat
      541 cgtgttatac aaatatttgg cgatgtatta taaaaaatat cttatcgcca aaaataaagg
      601 agattttgag aaagctgaaa tatataagaa tcccctgatt gaaatagtta aaattcgtga
      661 gattacgaaa acagaaaatg agtataaaac aatatcgaat tttgatagac gtgttttaag
      721 ggaatcagtt gaggaaatta atgaaaaatc aatgctgaat attgattttg ctaaagttag
      781 aaaaggcaga aattacaatt ttgctgtcca gtttaatatt tctagtgagt ttgatgaaaa
      841 aacttataga gaaaaaaaat taggttaatg caaaatatat cttactcgtt tgaagatgct
      901 tcagacgtat gctaagatat atagtgcctt gggagcaagg tattaatctc ctttagaggt
      961 taattagctt gtgatcgcct atcaattttt ttggtaggcg attctttttt ttgaaacaaa
     1021 aaagcaaaaa ttatttttta attagagagg tgcaatgcga tgaaatactt tttttgtgca
     1081 gtattcgaat ggaaaggaaa tgaatatgca gttaaattcc cagattttcc agacttatat
     1141 acacatggtg aaactttaga agatgctttt gagatggcgc aagaagtatt ggaaggatac
     1201 ttggtgcttt tagagttgca agaaaaagaa attccatcgc ctacgcctgt tcaagagtta
     1261 aatgcaagaa tcaagcgatc tgagaatgtt aatttatttg tcgtaaaagc taatacgaaa
     1321 gttgctaaag ctcgaaacaa gcaaaattat gatcgaaaat cagtgactgt ggaacactat
     1381 ttaaatgttt tagcggctga agagaatatt aatctgtcga aagctttgaa tgatttgttg
     1441 agagaaaagt tgatttgatg tctctcagat gatcatatta agcaaaaaca gcttgtttta
     1501 ggacgtatg
//
LOCUS       DACDXO010000150         1451 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E23605_2017
            SAMN12639989-rid9881463.denovo.162, whole genome shotgun sequence.
ACCESSION   DACDXO010000150 DACDXO010000000
VERSION     DACDXO010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639989
            Sequence Read Archive: SRR10023389
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741914.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:42:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,972
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 33 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 17 of 73
            Pseudo Genes (multiple problems)  :: 10 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1451
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639989-rid9881463.denovo.162"
                     /isolate="E23605_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            105..1406
                     /locus_tag="IWM35_002816"
     CDS             105..1406
                     /locus_tag="IWM35_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAP9920584.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTY
                     IVDRNCFIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWL
                     PRCLLMDEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKV
                     KVIVTDMNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQA
                     KYYRQLKALSRLLIKRQDKLVYDKWTKWRNFGWAYLTESEVVDRLLSISDELRIAYFY
                     YQEILQAFHDKEADTFFKLVRTMPNSVPKELHSIKKAFINYESGIRLALELPYSNAKI
                     ENLHTHIKALKRVAYGFRSFRKMKTRIFLLNNLITYESKNI"
BASE COUNT          476 a          293 c          264 g          418 t
ORIGIN      
        1 gatagaaaat cattctatcg gtcccttttg agcatgaaaa aacaaaacat acagaagctc
       61 cgttagaatt gaatcaccac aacacaatca aaggagcgtt ctgtatgcct gatcttcatc
      121 atatcaaact actacttgga atcaaagata aaaacatttt catcacaaat gtggaatcga
      181 agagcattaa gaatatcaag agtcttgttg tttctgcgac gttaagtaaa gaaattcgtc
      241 gctgtccttt gtgtaaacag atgaatcatg aaggcatgat cgttaagaac ggaaagaaaa
      301 aatcgctcat tcaacttaat aaatgtgcga accaactgac ttatcttgct ttagcgaaac
      361 aacgctacca ctgtcgtggc tgccacacct attttacggc gaatacctac attgtcgata
      421 gaaactgctt tatcgctaaa caagtgcgct ataaaatttt agaagagcta actgaaaaac
      481 aagcaatgac gacaattgct aagcattgcg gcgtttcttg gtcgaccgtt tctcggacac
      541 tcgcttctct gatccccatg acaaaagtaa aaagaaattg gttaccgcgt tgtttattaa
      601 tggatgaatt tcgttcactg aaaaatcaag tcggtcccta ctccttttct tgtatggatg
      661 gcgatactgg aaaattactg gatattcttc cttcacggaa gaaaaaggct ctcgtttctt
      721 attttatgca atttgaacga cgcgcacgat tgaaggtaaa ggtcatcgtg acggacatga
      781 atgtttctta tgcctctctg ataaaggaat gttttccaaa ggcaaaactc gttgtggatc
      841 gttttcacat tgtcaaacat ctcattcgta aatttgagga tattcgcgta cgaatcatga
      901 aaagctttga tcgaaacgat ccaacacaag ccaaatatta tcgacaatta aaggcgttgt
      961 ctcggcttct gatcaaacga caggacaagc ttgtttatga caaatggacc aaatggcgca
     1021 attttggttg ggcgtatctc accgaaagtg aagtggtgga tcgtctgctt tcgatctctg
     1081 acgaattacg aatcgcctat ttttactatc aagaaattct tcaagcgttt cacgacaaag
     1141 aggcggacac tttctttaaa cttgtaagaa cgatgcccaa ttccgttcca aaagaactgc
     1201 attctataaa aaaagctttt ataaactatg aatccggcat tcgtttagcc ctcgaattgc
     1261 cttattcaaa tgccaaaata gaaaatctgc atacccacat caaagcattg aaacgagtcg
     1321 cttatggctt tcgaagcttt cggaaaatga agaccagaat tttcttacta aataatctga
     1381 ttacctatga atcaaaaaac atctaaagta gaaaaggaat tctaccttag atgttcttat
     1441 ctcactctat c
//
LOCUS       DACDXO010000151         1451 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E23605_2017
            SAMN12639989-rid9881463.denovo.163, whole genome shotgun sequence.
ACCESSION   DACDXO010000151 DACDXO010000000
VERSION     DACDXO010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639989
            Sequence Read Archive: SRR10023389
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741914.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:42:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,972
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 33 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 17 of 73
            Pseudo Genes (multiple problems)  :: 10 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1451
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639989-rid9881463.denovo.163"
                     /isolate="E23605_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..625)
                     /locus_tag="IWM35_002817"
     CDS             complement(<1..625)
                     /locus_tag="IWM35_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HAP9920585.1"
                     /translation="MKTINLIKVFIASPGDIAAKRNEVEETIYRWNIENTDNTKVILM
                     PIRWETNAIPTYINNSSGQSVINKQIVENSDILLAIFGHKLGSPVDNYRSGTIAEIDY
                     FYKNSKGEVGVFFLDEPVPQVLAEEYIEVKKYRDSLEDKGFYGTYSKDNIRRFLTKKV
                     NYLIEKAQNNLESKRFDLDSISYKTPSVINKKVTGTDKDFDDNLDIFD"
     gene            complement(784..1050)
                     /locus_tag="IWM35_002818"
     CDS             complement(784..1050)
                     /locus_tag="IWM35_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9920586.1"
                     /translation="MMIKKLIEEKQKDKDFAEAYDREGRKLETAVALYHAREEAGLTQ
                     AELAQKAHTTQSTIARIERGDNVSFDKLDDIARALGKKLVVEFA"
     gene            complement(1040..1396)
                     /locus_tag="IWM35_002819"
     CDS             complement(1040..1396)
                     /locus_tag="IWM35_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAP9920587.1"
                     /translation="MEKISFETFKRPNGHDEFLEWLETLPKKDSAKLLRTIEETEKNG
                     MLVAQRLKWVKKLDTNLYELRSKVGSNIQRAIYFHVVNGRYVITHGFTKKTEKTPISE
                     LKHAKELREEWFTNDD"
BASE COUNT          405 a          279 c          181 g          586 t
ORIGIN      
        1 gatcaaaaat atctaagttg tcatcaaaat ctttatcagt tccggtaact tttttgttaa
       61 ttactgaagg tgtcttgtaa gaaatagaat ctaaatcaaa gcgttttgat tctaaattat
      121 tttgtgcttt ctcaattaaa taattcactt tttttgtgag aaaccttctg atattgtctt
      181 tgctatatgt tccataaaaa cccttatctt ctaaagagtc cctgtacttc ttaacttcta
      241 tatactcttc agccaaaact tgtggaactg gttcatctaa gaaaaaaacc cctacctcgc
      301 ctttgctatt tttataaaag taatcaattt ctgctatagt tcccgatcta taattatcaa
      361 ctggagaacc caacttatgg ccaaaaatcg ctaataatat atcactattt tctacaattt
      421 gtttattaat aacactttgc ccagagctat tatttatata agtaggaatc gcattcgttt
      481 cccatcgaat tggcatcaaa attacttttg tattatctgt attttctata ttccatctat
      541 atatggtttc ttctacttca ttcctctttg ctgctatatc acctggtgat gctataaata
      601 cttttattag atttatggtt ttcatttatt gtacctttct ctttatgtct ctttaaagta
      661 atttttgtat aaaatttatt gacttttaca ctataggaca acttttatct atttagtaca
      721 aaaaggccca ctaaactaat tgattagtta ggagcctatt acaggtatat tttcaactcg
      781 cttttaagca aattctacta ccagcttttt gcctaatgcc ctagcgatat catcaagttt
      841 atcgaaagat acgttatccc ctctctcaat tcgagcaata gtagattgag ttgtatgtgc
      901 tttttgtgca agctccgctt gagttaggcc tgcttcctcc cgtgcatgat aaagagcaac
      961 cgcagtttcg agttttcttc cttcacggtc atatgcttct gcaaaatctt tgtctttttg
     1021 cttttcttca atcaattttt taatcatcat ttgtgaacca ctcctctcta agttcttttg
     1081 catgtttaag ttcacttatt ggtgttttct ccgttttttt agtaaatcca tgggtaatga
     1141 cataccttcc attgaccaca tggaaataga tagctctttg gatgtttgat cctaccttac
     1201 ttcttaattc gtacaaatta gtgtctaatt ttttcaccca tttcaaccgt tgagctacta
     1261 gcatcccatt cttttctgtt tcttctattg tacgtaaaag cttcgcacta tctttttttg
     1321 gtaatgtttc caaccattct aaaaattcat catgaccatt aggtctttta aatgtttcaa
     1381 aagatatttt ttccataccc aattatatca tatattgatt ttatattata gtatataaca
     1441 taaaaattcg a
//
LOCUS       DACDXO010000152         1368 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E23605_2017
            SAMN12639989-rid9881463.denovo.165, whole genome shotgun sequence.
ACCESSION   DACDXO010000152 DACDXO010000000
VERSION     DACDXO010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639989
            Sequence Read Archive: SRR10023389
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741914.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:42:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,972
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 33 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 17 of 73
            Pseudo Genes (multiple problems)  :: 10 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1368
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639989-rid9881463.denovo.165"
                     /isolate="E23605_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            82..>1368
                     /locus_tag="IWM35_002820"
     CDS             82..>1368
                     /locus_tag="IWM35_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HAP9920588.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRA
                     CRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSK
                     KILPRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEE
                     VEFLVTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSPPLKEAY
                     PFFHELVEAFRDKDPDLFFSLLAELPETLDDSFREKLQNLLTYEEGITNAMIYPYSNG
                     KIEAKNTHIKTMKRVSYGFKSFENMRIRIFLINQLIN"
BASE COUNT          467 a          263 c          257 g          381 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcaccac aaaataaaca
       61 actgagagga cgatacgcta tatgaatgat tctatcaaaa aaatgctgag aataacagag
      121 aaagatttga tgattacaga ggtctcttac gagacccttc agaagaaaaa gacgttggtc
      181 gtcgatgctg ttctctcgcc tactcctcgt gcttgtagaa gttgtggttc tactgtggta
      241 gatggaaacg ggaaagcaat tatagtgaaa aatggaaaaa aggaaacgat tgtccgtttt
      301 gaacaataca atcatatgcc tttggttatg cgcctaaaaa agcaacgcta tacctgtaaa
      361 aactgtcgca cccattggac ggctcaaagt tattttgtcc aacccagaca ttcaatcgca
      421 aatcatgtta gatataaaat tgcttcttta ctgactgaaa aagtatcttt atcttttatt
      481 gcgaaaagct gtcaggtatc tttgaccacc gttattcgta cattgaaaga gtttaaaagc
      541 tatttaccaa agcaatctaa gaagatcctc cccagagtat tgatggttga cgaatttcgt
      601 tcgcatgctt ccatagaaga taagatgagc tttatttgcg cggatggcga aacaggaaaa
      661 ttaatagatg ttttgcctac gcgtaaatta cctcgattaa caagctattt cttaggttgt
      721 accaatccag aagaagtaga attcttggtg acagacatga acgccgccta cttccagctc
      781 accaaacgtg ttctgccaaa tgcgaaagtc gtgattgatc ggtttcatat tgtcaaacac
      841 atgaatcaag cgttcaatga gttgcgtatc cgtgaaatga atgaacttcg taaagccgga
      901 cagaaaagcc aggcagaaaa actgaaaaag aactggcgct ttttgctaaa aaatcgtgca
      961 aacatcaacc attatgaata caaaacatgg aaaagtttcc gagcaccaaa atacccattt
     1021 cttactgaag caatgatgat tgatcgattg cttgagtttt ctccgcccct aaaggaggcg
     1081 tatccctttt ttcatgaatt agttgaagcc tttcgagaca aagaccctga cttatttttc
     1141 tccttattgg cagaacttcc cgaaacgtta gatgacagct ttcgggaaaa gcttcaaaac
     1201 cttctgacct atgaagaagg catcaccaac gcaatgatct atccttattc caatggaaaa
     1261 atagaagcga agaataccca cataaagaca atgaaacgag tatcctacgg atttaaatca
     1321 tttgagaaca tgagaattag aatctttttg atcaatcaat taatcaat
//
LOCUS       DACDXO010000153         1355 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E23605_2017
            SAMN12639989-rid9881463.denovo.166, whole genome shotgun sequence.
ACCESSION   DACDXO010000153 DACDXO010000000
VERSION     DACDXO010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639989
            Sequence Read Archive: SRR10023389
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741914.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:42:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,972
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 33 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 17 of 73
            Pseudo Genes (multiple problems)  :: 10 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1355
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639989-rid9881463.denovo.166"
                     /isolate="E23605_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..>1355
                     /locus_tag="IWM35_002821"
     rRNA            <1..>1355
                     /locus_tag="IWM35_002821"
                     /product="23S ribosomal RNA"
BASE COUNT          362 a          295 c          415 g          283 t
ORIGIN      
        1 caagctgtga tggggaggga aataatagta ccgaagttcc tgatgtcaca ctgccgagaa
       61 aagcttctag tgagaaaaca gcggcccgta ccgcaaaccg acacaggtag tcgaggagag
      121 aatcctaagg tgagcgagag aactctcgtt aaggaactcg gcaaaatgac cccgtaactt
      181 cgggagaagg ggtgctgatc atacgatcag ccgcagtgaa taggcccaag cgactgttta
      241 tcaaaaacac aggtctctgc aaaatcgtaa gatgaagtat aggggctgac gcctgcccgg
      301 tgctggaagg ttaagaggag tgcttagcgc aagcgaaggt acgaattgaa gccccagtaa
      361 acggcggccg taactataac ggtcctaagg tagcgaaatt ccttgtcggg taagttccga
      421 cccgcacgaa aggcgtaacg atttgggcac tgtctcaacg agagactcgg tgaaatttta
      481 gtacctgtga agatgcaggt tacccgcgac aggacggaaa gaccccatgg agctttactg
      541 tagtttgata ttgagtgtct gtaccgcatg tacaggatag gtaggagccg tagaaatcgg
      601 aacgctagtt tcgatggagg cgctggtggg atactacccc tgcgttatgg ccactctaac
      661 ccgcaccact aatcgtggtg ggagacagtg tcagatgggc agtttgactg gggcggtcgc
      721 ctcctaaaag gtaacggagg cgcccaaagg ttccctcaga atggttggaa atcattcgaa
      781 gagtgtaaag gcagaaggga gcttgactgc gagaccaaca agtcgagcag ggacgaaagt
      841 cgggcttagt gatccggtgg ttccgcatgg aagggccatc gctcaacgga taaaagctac
      901 cctggggata acaggcttat ctcccccaag agtccacatc gacggggagg tttggcacct
      961 cgatgtcggc tcgtcgcatc ctggggctgt agtcggtccc aagggttggg ctgttcgccc
     1021 attaaagcgg cacgcgagct gggttcagaa cgtcgtgaga cagttcggtc cctatccgtc
     1081 gcgggcgttg gaaatttgag aggagctgtc cttagtacga gaggaccggg atggacttac
     1141 cgctggtgta ccagttgttc tgccaagggc attgctgggt agctatgtag ggaagggata
     1201 aacgctgaaa gcatctaagt gtgaagccca cctcaagatg agatttccca tttctttaag
     1261 aaagtaagat ccctgagaga tgatcaggta gataggtcag gagtggaagt acagtgatgt
     1321 atggagcgga ctgatactaa tcgatcgagg actta
//
LOCUS       DACDXO010000154         1340 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E23605_2017
            SAMN12639989-rid9881463.denovo.168, whole genome shotgun sequence.
ACCESSION   DACDXO010000154 DACDXO010000000
VERSION     DACDXO010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639989
            Sequence Read Archive: SRR10023389
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741914.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:42:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,972
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 33 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 17 of 73
            Pseudo Genes (multiple problems)  :: 10 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1340
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639989-rid9881463.denovo.168"
                     /isolate="E23605_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..1310
                     /locus_tag="IWM35_002822"
     CDS             <1..1310
                     /locus_tag="IWM35_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional aminoglycoside N-acetyltransferase
                     AAC(6')-Ie/aminoglycoside O-phosphotransferase
                     APH(2'')-Ia"
                     /protein_id="HAP9920589.1"
                     /translation="SLKKHYTEPWEDEVFRVIIEYNNVPIGYGQIYKMYDELYTDYHY
                     PKTDEIVYGMDQFIGEPNYWSKGIGTRYIKLIFEFLKKERNANAVILDPHKNNPRAIR
                     AYQKSGFRIIEDLPEHELHEGKKEDCYLMEYRYDDNATNVKAMKYLIEHYFDNFKVDS
                     IEIIGSGYDSVAYLVNNEYIFKTKFSTNKKKGYAKEKAIYNFLNTNLETNVKIPNIEY
                     SYISDELSILGYKEIKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMHGLDYTDISEC
                     TIDNKQNVLEEYILLRETIYNDLTDIEKDYIESFMERLNATTVFEGKKCLCHNDFSCN
                     HLLLDGNNRLTGIIDFGDSGIIDEYCDFIYLLEDSEEEIGTNFGEDILRMYGNIDIEK
                     AKEYQDIVEEYYPIETIVYGIKNIKQEFIENGRKEIYKRTYKD"
BASE COUNT          570 a          102 c          226 g          442 t
ORIGIN      
        1 aatcattaaa aaaacattat acagagcctt gggaagatga agtttttaga gtaattattg
       61 aatataacaa tgttcctatt ggatatggac aaatatataa aatgtatgat gagttatata
      121 ctgattatca ttatccaaaa actgatgaga tagtctatgg tatggatcaa tttataggag
      181 agccaaatta ttggagtaaa ggaattggta caagatatat taaattgatt tttgaatttt
      241 tgaaaaaaga aagaaatgct aatgcagtta ttttagaccc tcataaaaat aatccaagag
      301 caataagggc ataccaaaaa tctggtttta gaattattga agatttgcca gaacatgaat
      361 tacacgaggg caaaaaagaa gattgttatt taatggaata tagatatgat gataatgcca
      421 caaatgttaa ggcaatgaaa tatttaattg agcattactt tgataatttc aaagtagata
      481 gtattgaaat aatcggtagt ggttatgata gtgtggcata tttagttaat aatgaataca
      541 tttttaaaac aaaatttagt actaataaga aaaaaggtta tgcaaaagaa aaagcaatat
      601 ataatttttt aaatacaaat ttagaaacta atgtaaaaat tcctaatatt gaatattcgt
      661 atattagtga tgaattatct atactaggtt ataaagaaat taaaggaact tttttaacac
      721 cagaaattta ttctactatg tcagaagaag aacaaaattt gttaaaacga gatattgcca
      781 gttttttaag acaaatgcac ggtttagatt atacagatat tagtgaatgt actattgata
      841 ataaacaaaa tgtattagaa gagtatatat tgttgcgtga aactatttat aatgatttaa
      901 ctgatataga aaaagattat atagaaagtt ttatggaaag actaaatgca acaacagttt
      961 ttgagggtaa aaagtgttta tgccataatg attttagttg taatcatcta ttgttagatg
     1021 gcaataatag attaactgga ataattgatt ttggagattc tggaattata gatgaatatt
     1081 gtgattttat atacttactt gaagatagtg aagaagaaat aggaacaaat tttggagaag
     1141 atatattaag aatgtatgga aatatagata ttgagaaagc aaaagaatat caagatatag
     1201 ttgaagaata ttatcctatt gaaactattg tttatggaat taaaaatatt aaacaggaat
     1261 ttatcgaaaa tggtagaaaa gaaatttata aaaggactta taaagattga ttatataata
     1321 tatgaaaagc tattataaaa
//
LOCUS       DACDXO010000155         1334 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E23605_2017
            SAMN12639989-rid9881463.denovo.169, whole genome shotgun sequence.
ACCESSION   DACDXO010000155 DACDXO010000000
VERSION     DACDXO010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639989
            Sequence Read Archive: SRR10023389
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741914.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:42:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,972
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 33 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 17 of 73
            Pseudo Genes (multiple problems)  :: 10 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1334
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639989-rid9881463.denovo.169"
                     /isolate="E23605_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(61..279)
                     /locus_tag="IWM35_002823"
                     /pseudo
     CDS             complement(61..279)
                     /locus_tag="IWM35_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391850.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(545..739)
                     /locus_tag="IWM35_002824"
     CDS             complement(545..739)
                     /locus_tag="IWM35_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9920590.1"
                     /translation="MMGFFLRFYIIELTGRLTVFFGNFAIKPFAEVTAHYICKWLDCK
                     NKIKKVFHYISSSPCQIRPG"
BASE COUNT          476 a          219 c          179 g          460 t
ORIGIN      
        1 aggtattttt attatatata gatttaacat atgtcggtcg gttgattgat atagctaaag
       61 ttataactca accgaagccg attcatctac acgaataaag ttgcgagtgt tctcggctaa
      121 ttcataaaaa ttgttatgtg ttccatatag caatactgga ataactttat tttcttgaat
      181 ctcataagca actctataca cggtgaccgc atacttaaat ccattagacc aattacctga
      241 taaacctcca gttttttgct cttacttaaa tggatcaatg gcaatttcat cacaaaccta
      301 tagaggatga gtgacttctg cctaattacg ttaaaaaata ttgaaagtaa ctctatgtag
      361 caaaaaatat ctagaatttc atttcctcaa aactgatcga tattgtatct aaaaatatat
      421 actacctaaa ataggtagta tatattaaaa tatgtgcttt tgttagaaaa atcaatacat
      481 aaattatcat atctgtatat gtcatttagg tatttcagtt atgttataat agttttgctg
      541 tctgctaccc cggcctaatc tggcaagggg aggaggagat ataatgaaat acctttttga
      601 ttttgttttt gcaatcaagc cacttgcaga tgtagtgtgc agttacctct gcaaatggct
      661 tgattgcaaa attgccaaaa aagacagtaa gcctacccgt taattctata atatagaaac
      721 gtaaaaaaaa ccccatcagc ctgcacgctg atggggtttg gtgatataat gaaatacctc
      781 tttgttactc ataattgtac cataattagt cacgaatgca accaatagaa aaaactactt
      841 agaacggtag atcatcgcta ataacaatgc attgtccacc aaatggattt tgtgaagcat
      901 taaatacatt gttattaatt gtcgctgctt ttaactaggc ctctcccctt ctattataaa
      961 agaagatata tatcatttct taattaccta ctaccttaaa ccaaaattta agtactacct
     1021 aatttaagta ttacttaaac aatctaaaaa atagttatcc caattgttat gcttttataa
     1081 tacgattatt tgtaacaaaa tgaatattat ataattaaac actaccatat ttaggtagtg
     1141 tttaattatt agcaaaacaa aaaatattta ttctattctc ttaacttcaa ctaagttatg
     1201 ctgcacacct ttatataaat catatgcaac tcattagcta gacaaactac aagcatgtag
     1261 ttttatctgt taattatgta ctacctaaat aaagtagttt ataattagca agtatatata
     1321 tttaaaaatt tcat
//
LOCUS       DACDXO010000156         1324 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E23605_2017
            SAMN12639989-rid9881463.denovo.170, whole genome shotgun sequence.
ACCESSION   DACDXO010000156 DACDXO010000000
VERSION     DACDXO010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639989
            Sequence Read Archive: SRR10023389
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741914.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:42:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,972
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 33 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 17 of 73
            Pseudo Genes (multiple problems)  :: 10 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1324
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639989-rid9881463.denovo.170"
                     /isolate="E23605_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            278..820
                     /locus_tag="IWM35_002825"
     CDS             278..820
                     /locus_tag="IWM35_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9920591.1"
                     /translation="MLLKVAIKCYCVKNKGGIIYEKIVPRGACLLLALCLSLSAVGCQ
                     KSNENNKIKEGQSISSEEEGMSINANGKNETFKPSDYTLEAKKEYVYEYLGLKFKLSD
                     KFRNYIADKKIAMLDDQSPIDKELKYAILTFEKMTEEQKNAVIEKMGDGYKNWQNELE
                     RIGTIGIFEKNTSEEKNLKL"
     gene            910..>1324
                     /locus_tag="IWM35_002826"
     CDS             910..>1324
                     /locus_tag="IWM35_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HAP9920592.1"
                     /translation="MDELKRTEIQIIDKKERPKNGFVLSEKTDLENTEAFNKESVKDL
                     RKLPTKDINGKDFTSKDFEKYDLTMVNVFATWCTACVKEIPDLVEVQNEMKSKGVNIV
                     GVVTDPVDDNGENKEAIEKSKLIHEKTKASYPFLMP"
BASE COUNT          533 a          157 c          248 g          386 t
ORIGIN      
        1 catgaaaaat cctccagtgt gttttttatt attctacaca ccttattttt tctgtctagt
       61 ctagtgtagc cgattcaaat tgatgaagca gatcccaaag ggctgcttca tcaatttttt
      121 cgtaagtgtc cgtacactta ctgtgcttgc tcatatatta atcgatcata tactaatcgt
      181 gtaagaacaa ctttaagcat aatacttata ttgttatacc atagtttgga aaaataaaaa
      241 attatatttc atcaacgttt cataaaaggt gtgtagaatg ttattgaaag tagctataaa
      301 atgctattgt gtaaaaaata aaggaggaat aatttatgaa aaaatagtac caagaggagc
      361 atgcctatta cttgcattat gtttgagctt aagcgcagtt gggtgtcaaa aaagtaatga
      421 aaacaataaa ataaaagaag gacaatccat aagttctgaa gaggaaggca tgtcaattaa
      481 tgcaaatggc aaaaatgaaa cttttaagcc atcagattat acattggagg caaagaaaga
      541 atatgtttat gaatatttag ggcttaaatt taaactctca gacaaattta gaaattatat
      601 agctgataaa aagatagcga tgttagatga tcaaagtcct atagataaag aactaaagta
      661 tgctattttg acatttgaga aaatgacaga agaacaaaag aatgcagtta tagaaaagat
      721 gggcgatgga tataaaaatt ggcaaaatga gcttgaaaga attgggacta ttggtatatt
      781 tgaaaaaaat acatcagaag aaaaaaatct aaaattataa aatgtgatac tcatactaaa
      841 ataggagttt caagtgatgg aaaatacgat tgctatttaa gcacaaatag cggagctgaa
      901 agtaatcttc tggatgaatt aaaaagaact gagattcaaa ttatagataa aaaagaacgt
      961 cctaaaaatg gttttgtgct atcagaaaaa actgatttag aaaatacaga agcatttaac
     1021 aaagaatcgg ttaaagattt acgtaaacta ccaacaaagg acataaatgg caaggatttt
     1081 acaagtaaag attttgagaa gtatgatctt actatggtaa atgtatttgc aacttggtgt
     1141 acagcttgtg taaaggaaat tcctgatttg gttgaggttc agaatgaaat gaaaagcaag
     1201 ggtgtaaata tcgttggagt tgttacagat cctgttgatg ataacggtga aaacaaggaa
     1261 gcaattgaaa agtcaaagtt aatacatgaa aagacaaagg cttcatatcc tttcttaatg
     1321 ccag
//
LOCUS       DACDXO010000157         1314 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E23605_2017
            SAMN12639989-rid9881463.denovo.171, whole genome shotgun sequence.
ACCESSION   DACDXO010000157 DACDXO010000000
VERSION     DACDXO010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639989
            Sequence Read Archive: SRR10023389
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741914.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:42:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,972
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 33 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 17 of 73
            Pseudo Genes (multiple problems)  :: 10 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1314
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639989-rid9881463.denovo.171"
                     /isolate="E23605_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..848
                     /locus_tag="IWM35_002827"
     CDS             <1..848
                     /locus_tag="IWM35_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAP9920593.1"
                     /translation="EIADLIEKMYGHHYTPQTMSNITKSFTEEVTAFKGRELHDRYAA
                     IYMDATYIPLKRKTVAKEAIHIAVGIRPDGSKEVLSYAIAPTESITIWEEILLDLQER
                     GLKNVLLFITDGLKGMVGAISRFYPKARFQHCCVHVFRNISHKVRVNNRKEVCDDFKM
                     VYQASSKEVALEARGAFAKKWKTSYPKVVESILSNDHLLTFYDFPLAIRKSIYSTNLI
                     ESFNKQIKKYSHRKEQFQNEESMERFLVSSFDTYNQKFLGRSHKGFQQAEGELEQMLS
                     QPMEN"
BASE COUNT          429 a          240 c          254 g          391 t
ORIGIN      
        1 ctgaaattgc tgatttgatc gaaaaaatgt acggtcatca ctatactcca caaaccatgt
       61 ccaatataac aaaatcattt acagaagagg taacggcgtt taaagggcga gagcttcatg
      121 accgttatgc tgctatttat atggacgcaa cgtatattcc gttaaagcgg aaaaccgtcg
      181 ccaaggaagc tattcatatc gcagttggca ttcgcccgga cggatcaaag gaagtattga
      241 gctatgcgat tgcaccgact gaatccatca cgatttggga ggaaatttta ttagaccttc
      301 aagagcgcgg tttgaaaaat gtcctcctgt tcatcacgga tggcttaaag gggatggtag
      361 gagcgattag tcggttctat cccaaagctc gttttcaaca ttgttgtgta cacgtttttc
      421 gtaatatcag tcacaaagtg cgtgtcaata atcgtaagga agtctgtgat gattttaaaa
      481 tggtgtatca agcctcatct aaagaggtgg cgttagaagc acgtggtgct tttgcgaaaa
      541 aatggaaaac cagctatcca aaagtggttg aatcgattct ttcgaacgat cacttgctca
      601 ctttctatga tttccccttg gccatacgca agagtattta ttctacgaac ttgattgaat
      661 cctttaataa gcaaatcaag aaatacagcc accgcaagga acagttccaa aacgaagagt
      721 cgatggaacg tttcttagtc tcgtcttttg atacttacaa ccaaaaattc ctaggtcgca
      781 gtcataaagg tttccaacag gccgaaggcg agctcgaaca aatgctgagt caaccgatgg
      841 agaattagaa acaatctaca gtagggaaga accatttaca caaaattatt gacgctccca
      901 agctaaaact cctattaatc gatctactaa aaataaataa aaaaattctt ttcaattttt
      961 atttatggta tgacataaat aaagtaaagt tattcttcta ggttaatctt attaatactc
     1021 tttctcacta gtgttgcatg caacactagt gagaaagagt attaaatata ctgcaaggca
     1081 agacatttgg taattgaaga ataacaagca aaaaaatagc agtcaggtct cttgacaaag
     1141 tatagttgct attctttttt taggccctac tttaatgaaa tttttgtttg gagggaacga
     1201 cttcattcct ttgattattt cagttgaatt taatagagaa gcctggcgtc tattaagaaa
     1261 tacttttctt gatgagcgtc aattttttta acggaaatct atcctatact aaat
//
LOCUS       DACDXO010000158         1310 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E23605_2017
            SAMN12639989-rid9881463.denovo.172, whole genome shotgun sequence.
ACCESSION   DACDXO010000158 DACDXO010000000
VERSION     DACDXO010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639989
            Sequence Read Archive: SRR10023389
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741914.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:42:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,972
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 33 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 17 of 73
            Pseudo Genes (multiple problems)  :: 10 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1310
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639989-rid9881463.denovo.172"
                     /isolate="E23605_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(150..>1310)
                     /locus_tag="IWM35_002828"
     CDS             complement(150..>1310)
                     /locus_tag="IWM35_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HAP9920594.1"
                     /translation="SEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQYIQANAYER
                     SEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKALIAAMIEMV
                     ISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGSEYPFFMCDA
                     LYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQSFVQRGLFG
                     VKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVKDELKSIFKA
                     SELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRRRIRTTNVLE
                     RLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNSNNI"
BASE COUNT          380 a          290 c          232 g          408 t
ORIGIN      
        1 taaaagttca acctcatcaa aagcacaatg ttttgcgttg tgaacctaac tgccatgcct
       61 ttctttctga ggagagaggt caggagcagt agcgcagcga ctcttgatgt ctctcctcag
      121 aaagaagaga ctcgcacagg ttcatggtgc taaatgttat tcgaattaaa ttctaaatat
      181 tttcgtggag aagccagcca ctcagtgtct ttttccatca aaagtgttcc aattatccga
      241 gtgatggaat tgatatttgg aaaaatccga atcactcgtt ctctccggcg aatctcttcg
      301 ttcaaccgtt ccaacacatt ggttgtacga atccttcggc ggatattttc aggaaaagac
      361 aaaatttgaa tagcatcttc aaagccgttt tctaaaatgt cgcaagcagc acttaatttt
      421 gaatcacaac catatttttc caagaagtgc tcttttcttt cacgtgtcaa ttcaagttca
      481 gaggctttaa agatactctt caactcgtct tttacatcag aagaaacttt ctttggcagc
      541 ttatcaaaaa tatttctcaa aaagtgggct tggcatcttt gccagctcgc acctaaaaag
      601 ttcttgcgaa cagccttaac taaccctttg tgtgcatcag aaatgaccag ttttacaccg
      661 aacaaacctc gttgaacgaa cgattgaaaa acggtatccc aattatcttc cgattcgccg
      721 tcttgaacat caaaaccaag aatcgtacgt cgtccatcag aatcaatgcc gataccaata
      781 tatacacctt ttgagacaat acgatgattt tcccttactt tcatatacag ggcatcacac
      841 atgaaaaatg gatattccga gccttctagg ctccggtttc tccactcaaa aacaaatgga
      901 tccaactgtt tcatcagatt tgaaacgaat gatttagaaa cagtcgcacc atccgttagc
      961 agttcaactg ttttagttac tttgcgagtg gagacaccag aaatgaccat ttcaatcatg
     1021 gcagcgatca aagctttttc gcttcgctga tatctttcaa agatagtagg actaaactta
     1081 ccgtctctgg ttcgtggaac atgaagttct aacgtaccaa ttcgagttgt aaacgaacga
     1141 tcgtagtaac catttctttg tgattgccgt tcttctgaac gttcataggc atttgcctga
     1201 atatattgat ttcgttgttc ttccatcagc tgattaaaca tgatagtcat aagtgttttt
     1261 gcggtatcat tcgcaccgct ttcgtttata attgcttgaa tttcttcgct
//
LOCUS       DACDXO010000159         1306 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E23605_2017
            SAMN12639989-rid9881463.denovo.173, whole genome shotgun sequence.
ACCESSION   DACDXO010000159 DACDXO010000000
VERSION     DACDXO010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639989
            Sequence Read Archive: SRR10023389
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741914.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:42:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,972
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 33 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 17 of 73
            Pseudo Genes (multiple problems)  :: 10 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1306
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639989-rid9881463.denovo.173"
                     /isolate="E23605_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            24..>1306
                     /locus_tag="IWM35_002829"
     CDS             24..>1306
                     /locus_tag="IWM35_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAP9920595.1"
                     /translation="MSYSEFTKELLDILDLNLTFHEDAFRKERINDETCFVFDGTLTY
                     QPEECFHCHYQNKQTIIKWGWKKVSILLNDVSNYKTIVRINKQRFKCKHCGKTFLAED
                     SVSDRRCSIARRVKQAILELLSEPISMSLIARMKHISPTTVIRILRSLRPKTVSLNQP
                     LPEVVCFDEFKSVKNVSGAMSFVMMDGKTHQLIDIVENRQLNPLRDYFLRYPRKVREQ
                     VRLVVSDFYTPYRTLVKECFPNARIVADRFHISQHIGRAFTNHRIQVMKTFKKGDRRS
                     KHLKKYWRLLQKNAWELNGQHRYWRPSFRDHLTEAEIVDRLLYYDDSLKRGYEVYQVF
                     LSAIRRQDVPEFVALLKEDYKELPEHYQPVFTTFKKYRTEIKRALRVPYSNGPIECLN
                     NHIKVLKRIAYGFRNFQNYRERIFLYRGKYFKKTK"
BASE COUNT          407 a          285 c          261 g          353 t
ORIGIN      
        1 accattcaaa ggagtgtttc aagatgtcct attcagagtt taccaaagag ttactagata
       61 ttctagacct aaatcttacc tttcatgaag atgcctttcg caaagagcgc attaatgacg
      121 aaacatgttt tgtttttgat ggcacattga cctatcaacc agaggaatgc ttccattgcc
      181 actatcaaaa taagcaaacg atcattaaat ggggctggaa aaaagtatcc atcttattga
      241 atgatgtcag taactacaag accattgtgc ggattaacaa acagcgcttc aaatgtaaac
      301 actgtggaaa gactttttta gcagaagatt cagttagcga tcgtcgctgc tccattgccc
      361 gcagagtcaa acaagccatt cttgagctat taagtgagcc tatttctatg tctttgattg
      421 cacgtatgaa gcacatttct ccgaccactg tcattcgaat acttcgcagc cttcggccta
      481 aaaccgtttc tttgaaccag ccgttaccag aagtggtgtg tttcgatgaa ttcaaatcgg
      541 tgaaaaacgt ctcgggagcc atgagctttg tcatgatgga cggcaagacc catcaactga
      601 ttgatattgt ggaaaatcgg caactcaatc ctttacgcga ctatttcttg cgctacccta
      661 gaaaagtccg tgaacaagta cgtctagtgg tttcagattt ctatacaccc tatcgcacat
      721 tggtgaaaga gtgttttccg aatgcccgaa tcgtcgccga tcgctttcat attagccaac
      781 acatcggtcg ggcctttacc aaccaccgaa ttcaagtaat gaagaccttt aaaaaagggg
      841 atcgtcggtc gaagcactta aagaaatact ggagactact tcaaaagaat gcgtgggaat
      901 tgaatggtca gcatcgctac tggcgtccgt catttcgaga tcatctgaca gaagcggaga
      961 tagtcgatcg gctattatat tatgatgact ccttaaagcg aggctacgaa gtctaccagg
     1021 tttttctttc cgctatcaga agacaagatg ttcctgaatt cgtcgcgctc ctaaaagaag
     1081 actacaagga gttgccagag cactatcaac cagtgttcac gaccttcaag aaatatcgaa
     1141 cagaaatcaa acgagcgttg cgagttccct attccaacgg ccctatagaa tgtttgaaca
     1201 accatatcaa agtactaaaa cgaattgcct atggctttag aaactttcaa aactaccgag
     1261 aacgaatctt tttatatcga ggaaaatatt tcaagaaaac taagaa
//
LOCUS       DACDXO010000160         1296 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E23605_2017
            SAMN12639989-rid9881463.denovo.174, whole genome shotgun sequence.
ACCESSION   DACDXO010000160 DACDXO010000000
VERSION     DACDXO010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639989
            Sequence Read Archive: SRR10023389
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741914.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:42:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,972
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 33 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 17 of 73
            Pseudo Genes (multiple problems)  :: 10 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1296
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639989-rid9881463.denovo.174"
                     /isolate="E23605_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            141..398
                     /locus_tag="IWM35_002830"
     CDS             141..398
                     /locus_tag="IWM35_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9920596.1"
                     /translation="MSNLENKEEKVVNKIVSVVNKLDKELDELDTLSENPEKKHNLKK
                     WLVERKAIHEIKKVLHEADKYEKYDEKELDKEFKEINDLLL"
     gene            complement(630..1043)
                     /locus_tag="IWM35_002831"
     CDS             complement(630..1043)
                     /locus_tag="IWM35_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014834562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4231 domain-containing protein"
                     /protein_id="HAP9920597.1"
                     /translation="MDQKAFIESIDSTIDRLKKEIRFYNRVIGIGNIIKIVLSASIPI
                     LIDQASEHRSLLLIVSIASAIITIIQSGMSAFNYQDKVQTSTELLMKIEKEKLLYITK
                     TSPYNKTDEENFHLIVTTLQTELNDIISDFNQVNN"
BASE COUNT          489 a          165 c          162 g          480 t
ORIGIN      
        1 ttctgtccac gttcatttta ctccaaagtt ttcctcattt caaaagaaaa tcacagaata
       61 cacgaagaag cgtttctatt tgtataaact atatcaaagt tctatactta aattatcaaa
      121 taaaaaggac gtgaagataa atgtcaaatt tagaaaacaa agaagaaaaa gttgtaaaca
      181 aaattgtttc tgttgtcaat aaactggata aagagttaga cgaacttgat acgttatctg
      241 aaaatccaga aaaaaaacat aacctaaaaa aatggttagt tgaacgaaag gctattcatg
      301 aaattaaaaa agttcttcat gaagctgata aatatgaaaa atatgatgaa aaagaacttg
      361 ataaagaatt taaagaaatc aatgacttat tactataatt gatttatacg actcattttt
      421 gagtcgtcag actgcaaaca acactctaaa tttaggagtt tgtctacagt ctgaatagat
      481 tttaaattta ttgtccataa tgttattatg aatttatagc attaagaaaa gagataagac
      541 tttacatttt tttaggaatg taaagtctta tctcttttct taattgtaca attagtatta
      601 tctgcactac tttctaatta aataaatttt tagttattaa cttgattaaa atcagagata
      661 atatcattta attcagtttg aagggttgta acgattaaat ggaaattttc ttcatctgtt
      721 ttattatatg gggatgtttt cgttatatat aacaactttt ctttttcaat tttcattaat
      781 aactctgtcg aagtctgtac tttatcttga taattaaatg cagacatacc actttgtata
      841 attgtaatta ttgctgaagc aattgaaaca atcaatagta aagacctgtg ttcggaagcc
      901 tgatcaatta atattgggat actagctgat aagactattt ttataatatt tccgatacca
      961 attactctat tataaaatcg aatttctttt tttaatctat cgattgtaga atcaatagat
     1021 tcgataaacg ctttttgatc catcattttc tctccaatca ttttattaaa atcattaaca
     1081 aaaatattat aataggattt aagtaagttc taactaatca catataaaaa taaaaaaaca
     1141 tttgtatttt atcaaagttt tattatactt gtcaatttgt gtacatccta aaatataagc
     1201 tatttgttta cttctgtaga atatatactt tgttgttaga taagttgcac atgtttatag
     1261 aatctaaata ataaattttt atgtaccgcc cctcaa
//
LOCUS       DACDXO010000161         1294 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E23605_2017
            SAMN12639989-rid9881463.denovo.175, whole genome shotgun sequence.
ACCESSION   DACDXO010000161 DACDXO010000000
VERSION     DACDXO010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639989
            Sequence Read Archive: SRR10023389
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741914.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:42:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,972
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 33 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 17 of 73
            Pseudo Genes (multiple problems)  :: 10 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1294
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639989-rid9881463.denovo.175"
                     /isolate="E23605_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..1244
                     /locus_tag="IWM35_002832"
     CDS             <1..1244
                     /locus_tag="IWM35_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HAP9920598.1"
                     /translation="ITEKEHLLPLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCL
                     THYQHTYCCKSCEKEGLSSFKKAIVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQE
                     NYWKQTHGLDISRDNITNWHIKAVQNALDPLGERLRVYLNQEEILHGDETSYRVIESA
                     KTDTYYWQFCTGKDSQHPIVYYHHDESRAGDVPKTFLKEFTGYLHCDGYSGYNAVESV
                     RLVYCFAHVRRKFFEAIPKGKKNTDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQ
                     QELRPLFIAFYEWMATIDPVAKSKLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERN
                     IKELVIGRKNWLHSTSLEGARTSGIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVLS
                     AETLDQLLPWNKDVQQHFSRN"
BASE COUNT          423 a          233 c          277 g          361 t
ORIGIN      
        1 ctataacaga gaaagaacat ctccttcctt tggaagaaca attttgtgaa cattgcggtt
       61 cacagatgaa agatatcgga cggacaaaag tacgagaaga aattcgtttc catcaggcta
      121 tgttggattg tctcacccat tatcaacata cctattgttg taaaagctgc gaaaaagaag
      181 gactttcttc ttttaaaaaa gcgatcgttc ctaaaccttt gatttctaat agcttaggtt
      241 ccaatagttt agtggcggaa acgatccgga tgaaatttgg tcagaaagtt cctgcctatc
      301 ggcaagagaa ctattggaaa caaacacatg ggttggatat ttcgagagat aatatcacca
      361 attggcatat taaagcagtg caaaacgcgt tagacccttt aggagaacga ttaagagtct
      421 atcttaatca agaagagatt ctacacgggg atgaaacaag ttatcgtgtg attgaaagtg
      481 ccaaaacaga tacctattac tggcagtttt gtacaggaaa agacagtcag catcccatcg
      541 tttattatca ccacgacgaa agtcgtgctg gagatgttcc gaaaactttt ctcaaggagt
      601 ttacagggta tttacactgt gacggctata gcgggtataa tgccgtggaa agtgtacgac
      661 ttgtctattg tttcgcgcat gttcggcgga aatttttcga agcaattcca aaaggaaaga
      721 aaaacacgga tattcccgca gctcaagcag tcaaacaatt ggataaatgg tttgtcctag
      781 aaaagaaatg gaaagacttt tctcctgaga agcgattaag ctgtcggcaa caagaactgc
      841 gtccattatt tattgctttt tatgagtgga tggcaacgat agatcctgtt gcaaaatcca
      901 agttggatgc agccgttcaa tatgcgtgta aactacgaag tggttttgaa cctatttttg
      961 aagatggtcg tttagaactc acaaataatc gtgcggagcg aaatatcaaa gagctggtga
     1021 taggacggaa aaattggttg cattccacaa gtcttgaagg agcacgaact tcaggaatca
     1081 ttctgagtgt atataaaaca gcagaactta acggattgaa tcctgtaaag tatctagagt
     1141 ttctatttga taagatacca aatcttcccg tactttcagc agaaacttta gatcaactgt
     1201 tgccttggaa caaggatgta caacaacatt tttctcgtaa ctaaaaacgc caaacattcc
     1261 cgattctatt ctataggtcg ggttatttga cgta
//
LOCUS       DACDXO010000162         1269 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E23605_2017
            SAMN12639989-rid9881463.denovo.176, whole genome shotgun sequence.
ACCESSION   DACDXO010000162 DACDXO010000000
VERSION     DACDXO010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639989
            Sequence Read Archive: SRR10023389
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1269)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741914.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:42:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,972
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 33 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 17 of 73
            Pseudo Genes (multiple problems)  :: 10 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1269
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639989-rid9881463.denovo.176"
                     /isolate="E23605_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            24..1103
                     /locus_tag="IWM35_002833"
     CDS             24..1103
                     /locus_tag="IWM35_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP9920599.1"
                     /translation="MTQTHDNTVSRKGKHLSYSERCQIAILKKEGYSNRQVAKVLGRV
                     PQTINNEIDRGTITQLKRQTQNGKVYDTYYSVYDADAGQAFYEKQRLNCGRRPKWANT
                     NAFIDWADDKMLNEKWSPDVVVGFARKHELFDCAIIPGTTALYQWIDRGIMRTKNMDL
                     LEKLSRKIKAVRPGHRPNKRVLGTSIEERPKEVETRETFGHWEIDTVVGNKTKSDAVL
                     LTLAERQTRFEVILKLNGKDTESVDQAVQQLRERAGANFDQLFKTVTSDNGSEFSGLH
                     DALQDVADVYFSHPYASWERGTSENQHKLIRRFVPKGSPISEVSENQILRIQRWMNDY
                     PRKILGYATPHDTFVQAFKQERLVA"
BASE COUNT          378 a          302 c          310 g          279 t
ORIGIN      
        1 tcaatagaca aggatgaatt cacatgacgc aaacccatga taacacagtt tcacgcaagg
       61 gaaaacatct ttcctactca gaacgttgcc aaatcgctat cctgaaaaag gaaggctatt
      121 ccaaccgtca ggttgccaaa gtgctgggac gtgttccgca aaccataaac aatgagattg
      181 atcgcggaac catcacccag ttaaaacgcc aaacgcaaaa tggaaaagtg tatgacacct
      241 actattctgt ttatgacgct gatgcaggac aggccttcta tgaaaagcaa cgactgaact
      301 gtggcagacg ccccaaatgg gcgaacacaa atgccttcat tgattgggca gatgacaaaa
      361 tgttaaatga aaaatggtcg ccggacgtcg tggtcggatt tgccagaaaa cacgagctgt
      421 tcgattgtgc catcattcct ggcacaaccg ctctttatca atggattgac cgcggaatca
      481 tgcgaacaaa gaacatggac ttgcttgaaa aactgtcccg taaaatcaaa gcggtccgcc
      541 caggacaccg tcccaacaag cgtgttctcg ggacgtcaat tgaagaacgc cctaaagaag
      601 ttgaaacacg ggaaacgttc ggtcattggg aaatcgacac ggtagtggga aacaagacca
      661 agtcggatgc cgtgctgctg acgctggctg aacggcagac acgctttgaa gtcatcctaa
      721 agttgaacgg gaaggacacc gaatcggtgg accaagcggt tcaacagcta agagaacggg
      781 ctggcgctaa cttcgatcaa ttatttaaga ccgttacatc cgacaatggc tcggagtttt
      841 ctgggctaca cgatgctctg caagatgtag ctgacgtcta tttcagccac ccctatgctt
      901 cttgggagcg tgggacgagc gaaaaccagc acaaattaat tcgccgtttc gtccccaagg
      961 gaagcccgat tagtgaggtg agcgagaacc agattttgcg gatccagcgt tggatgaacg
     1021 attacccgcg gaaaattttg ggttatgcga caccacatga tacattcgtc caggcattca
     1081 agcaagaacg gttggtggct tgacccatta ttccttgata acgatcctca ctcgggacat
     1141 ggcttcatgt ctggaaagct ggcctggggc cagaatcgtc tgaagactat catacctcag
     1201 ttcattatca aggcctcact gatttagaat aacgaggcag ttacggtatc aaacaaatga
     1261 gttacctgt
//
LOCUS       DACDXO010000163         1264 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E23605_2017
            SAMN12639989-rid9881463.denovo.177, whole genome shotgun sequence.
ACCESSION   DACDXO010000163 DACDXO010000000
VERSION     DACDXO010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639989
            Sequence Read Archive: SRR10023389
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741914.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:42:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,972
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 33 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 17 of 73
            Pseudo Genes (multiple problems)  :: 10 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1264
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639989-rid9881463.denovo.177"
                     /isolate="E23605_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            24..1103
                     /locus_tag="IWM35_002834"
     CDS             24..1103
                     /locus_tag="IWM35_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006908283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP9920600.1"
                     /translation="MTQNHDNTVSRKGKHLTYSERCQIAILKKENYSNRQVADVLGCV
                     PQTINNEIKRGTVTQLNRQNQNGKVYDYYHDVYDADAGQAAYEKNRLNCGRRPLWADT
                     DAFIEWADDMMLLEKWSPDVVVGFARKHELFDPAIIPGTTTLYQWIDRGIMKTKNMDL
                     LEKLSRKPKSSRHGNRPNKRVLGKSIEERPEAVGTRKTFGHWEVDTVVGNKTKSDAVL
                     LTLVERQTRFEVILKLAGKDAESVNQAVRQLRERAGAYFGQLFKTVTSDNGSEFAGLH
                     DALQDVANIYFSHPYASWERGTSENQHKLIRRFIPKGSPIGNVSENQILRIQRWMNDY
                     PRKILGYATPHDTFVQAFRQERLTA"
BASE COUNT          335 a          343 c          355 g          231 t
ORIGIN      
        1 tcaatagaca aggatgaatt cacatgacgc aaaaccatga taacacagtt tcacgcaagg
       61 gaaaacacct aacctactca gaacgttgcc aaatcgccat cctgaaaaag gaaaactatt
      121 ccaaccgtca ggttgcggat gtcctgggct gcgtgccgca gaccataaac aacgaaatca
      181 aacgcgggac cgttactcag cttaaccgcc agaatcagaa cgggaaggtc tacgactatt
      241 atcatgacgt ctacgacgcc gatgccgggc aggcggccta cgagaagaac cgcttgaact
      301 gcggcagacg gcccttatgg gccgacacgg acgccttcat tgaatgggcg gacgacatga
      361 tgctgttgga aaaatggtcc ccggacgtgg tggtgggctt tgccagaaaa cacgaattgt
      421 tcgacccggc cattatccct gggaccacca ccctctatca gtggatcgac cgtggcatca
      481 tgaaaacgaa gaatatggac ctgctagaaa aactgtcccg caaaccgaaa tcgtcccgtc
      541 atggcaaccg cccgaataaa cgggtgctag ggaaatccat tgaagagcgc ccggaggcgg
      601 ttggtacccg gaaaaccttc ggccattggg aagtcgacac ggtcgtagga aacaagacca
      661 agtcggatgc cgtgctgctg acgttggtgg aacggcagac acgcttcgag gtcattctga
      721 agctggccgg gaaggatgcc gaatcggtca accaagcggt ccgacagctg agagaacggg
      781 ctggtgcgta cttcggccag ctgttcaaga cggttacctc ggacaacggc tcggagttcg
      841 cgggactgca cgatgccttg caggatgtgg caaacatcta cttcagccac ccctatgctt
      901 cctgggagcg cgggacgagc gaaaaccaac acaagctgat tcgccgtttc atccccaagg
      961 gcagcccaat cggcaacgtg agcgaaaacc aaatcttgcg gattcagcgt tggatgaacg
     1021 attacccgcg aaagattttg ggctatgcaa cgccgcatga cacgttcgtc caggcattta
     1081 ggcaggaacg gttgacggct tgacacgtgg gcccttgaca acgagcctcc ctcggcatgg
     1141 cttgtgtctg gaaagctggc cggggccagc cagtcgggag actaccatgc ctcagctcat
     1201 tgtcaagggc ccactcgatt agaaaaatta gacggttggt ggtaacgagc aaccgggtca
     1261 ccat
//
LOCUS       DACDXO010000164         1223 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E23605_2017
            SAMN12639989-rid9881463.denovo.178, whole genome shotgun sequence.
ACCESSION   DACDXO010000164 DACDXO010000000
VERSION     DACDXO010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639989
            Sequence Read Archive: SRR10023389
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1223)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1223)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741914.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:42:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,972
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 33 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 17 of 73
            Pseudo Genes (multiple problems)  :: 10 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1223
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639989-rid9881463.denovo.178"
                     /isolate="E23605_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            422..955
                     /locus_tag="IWM35_002835"
     CDS             422..955
                     /locus_tag="IWM35_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HAP9920601.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
BASE COUNT          390 a          226 c          229 g          378 t
ORIGIN      
        1 tggttggact tattaccgca gatgcaagct tttgaacacc gtttattgag aagttgtgaa
       61 atcagcgtcc tgacctgagt attagagaaa caaatggcaa aatagttcct catattttgc
      121 tattttttga gctaataccg agggtcagct gattgaacac cgtttagtgg agaagttgtg
      181 aaaaagtcgc ctactttcga gtcgtaagaa atttttttga aaatggttcc tcacaacttt
      241 gcataaattt gacttaatac cgagaagtca tgatccccat ctaactccaa atcacaagga
      301 atgagttcaa aaactagctt ttctcaacaa atattacata aaaaattcta tgttttttgt
      361 taaatttatt tgcttttttc aaaagtatgt tacagtaatt tcccaaggag gttttacaga
      421 aatgaatttt gttgccatgg attttgaaac tgccaatcat cagccttata gtgcttgttc
      481 tcttgcgcta gtcatggtaa aaaatagcca gatcgttgat gaattctata cattgataca
      541 gcctgagact ccttttttct ggcgaaatgt ccaaattcac ggaatccatc aagaagacgt
      601 acgcaatgcg ccgaaattcc cagaagtctg gcaaactatc caaccatatt ttcaaaaaaa
      661 tcgtttagtc gttgcacata atgctgcttt tgatacaaag gtcttagcgg gatgcttaga
      721 ttattatcat cttgaacagc ctaattatct ttctttatgt acagtgaaaa gtagccgtcg
      781 cctgtttcct gaatttccaa atcatcgatt gaacactgtg tgcgaaaatt tgaacatcca
      841 attagatcat caccatgatg cattggaaga tagtcgtgca tgtgcggaaa tcctgcttta
      901 tcaagaaaaa aaattcggga cagatccatt gaaaaaactt gtgactataa agtaaaagaa
      961 tacttatgaa acacacaaag aggtcgtgag cctgccgttt cactccgagt cacaaggagc
     1021 aatttcaaaa aatagcttct catgtttatt aaatattgtg actggtgacc gaggagctgg
     1081 caggagaaca ccgtttattg gagaggttgt gaaaaagctg ttctgcatca agtaataaga
     1141 acagccaaac aaaaatagct tctcatattt ttcatttggt tggacttatt accgcagatg
     1201 caagcttttg gacaccgttt acc
//
LOCUS       DACDXO010000165         1193 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E23605_2017
            SAMN12639989-rid9881463.denovo.180, whole genome shotgun sequence.
ACCESSION   DACDXO010000165 DACDXO010000000
VERSION     DACDXO010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639989
            Sequence Read Archive: SRR10023389
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741914.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:42:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,972
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 33 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 17 of 73
            Pseudo Genes (multiple problems)  :: 10 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1193
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639989-rid9881463.denovo.180"
                     /isolate="E23605_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..1076
                     /locus_tag="IWM35_002836"
     CDS             <1..1076
                     /locus_tag="IWM35_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAP9920602.1"
                     /translation="VIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFSR
                     MFDENKTDRNDALRIADFLRIQRFTTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQHF
                     LENLTYKCNTLAREMRDESTSLFSATLISLMTEDFTLDELAELPLEAFCDLLQEKGKG
                     RFKQPEKIAKAIQRAITMSYRLGALAQESINVVLSVLVREIRALEKNIKELDKAIEQV
                     IVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIERFEDQTKLAKYAGLSWKVNQSGNYQS
                     QNTPLTKQGNRYFRYYLVEAANSVKNYLPEYKAFYQSKYKEVPKHQHKRALVLTARKF
                     TRLVDTLLRKHQLYTPPRSVIEK"
BASE COUNT          418 a          234 c          210 g          331 t
ORIGIN      
        1 ttgtgattgg catggagtca acgtccatgt acagcttcca tccttccatg ttttttcatg
       61 aagatgagaa actaaagaag ctcaacacac ttgtaacgat tgaaaatcct tttcgggtga
      121 aacaatttag tcgcatgttt gatgagaata aaactgatcg aaacgatgca ttaagaatcg
      181 ctgatttctt gcgtattcaa cgattcacaa cttcccctat caaagaagag aaatacatgg
      241 ccttacagcg tttaacacgt actcgctatc aacttattaa gcaattgatt cgtacaaagc
      301 aacattttct tgaaaatcta acgtacaaat gtaatacgct tgctcgagaa atgcgagacg
      361 aatcgacgag tctttttagt gcaaccctca tttcactcat gacggaagat ttcacattgg
      421 atgaacttgc tgagcttcct ttagaagctt tctgtgattt acttcaagaa aaaggaaaag
      481 gccgtttcaa gcaaccagaa aaaatcgcta aagcaattca acgtgcaatt accatgagtt
      541 accgcctagg tgctcttgca caagaatcaa ttaatgttgt tttaagtgtt ctcgtacgag
      601 aaatacgagc acttgaaaaa aatatcaaag aattagataa agccatcgaa caagtaattg
      661 ttgtcattcc agaatatcaa tgcttaacca gtattccagg cgttggtaaa gtctatgctg
      721 ccggtttaat cgctgaaatt ggtcaaatcg aaagatttga agatcagaca aaattggcaa
      781 aatacgctgg attaagttgg aaagtgaacc aatctggaaa ctatcagtcg caaaatacac
      841 ctctgacaaa acaaggaaat cgatatttta gatactactt agttgaagct gccaactctg
      901 taaaaaatta tcttcctgaa tacaaagctt tttatcaaag taaatataag gaggttccaa
      961 agcatcaaca caaacgtgca ctcgtcctaa ccgcaagaaa atttacgcgt ctggtggata
     1021 cgctactacg taaacaccaa ctctatacgc caccaaggag tgtgatagag aaataacaat
     1081 ccgttattga cgcaaatcct tgaatcaacc cgaaaaaaat ttgattttga tcgggtctag
     1141 tttcgtgcgc ttcaaaacat attactcaat aaagaaaatc ttaaatttca tct
//
LOCUS       DACDXO010000166         1176 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E23605_2017
            SAMN12639989-rid9881463.denovo.181, whole genome shotgun sequence.
ACCESSION   DACDXO010000166 DACDXO010000000
VERSION     DACDXO010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639989
            Sequence Read Archive: SRR10023389
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1176)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1176)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741914.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:42:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,972
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 33 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 17 of 73
            Pseudo Genes (multiple problems)  :: 10 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1176
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639989-rid9881463.denovo.181"
                     /isolate="E23605_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            153..938
                     /locus_tag="IWM35_002837"
     CDS             153..938
                     /locus_tag="IWM35_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SinI family restriction endonuclease"
                     /protein_id="HAP9920603.1"
                     /translation="MPNKNRTFESILNEGLTTEPASIIFDEVIDDVEDSKYHKNLKEL
                     FYFALNNGPSLFTGISVPDSDNPLIMAKIYLTKWCNKYIRDRNNPALKKPLKTFGEKD
                     AALTTRVAANANIDDQKVLNDYLRGHFLYMSAENMNGSILEEYLAEVLEPEGWIWCAG
                     SVYRAVDFCYLGTSPILLQVKNKYNTESSSSSAIRVGTTIRKWNRLNKSTKISGLDSP
                     IPNWKALIEMTEASKELAAKLTENSYLAYINEKSTRELWTLDD"
     gene            931..1173
                     /locus_tag="IWM35_002838"
     CDS             931..1173
                     /locus_tag="IWM35_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9920604.1"
                     /translation="MTNNILISIYPRYMEKIINKQKNFEFRNFYLTSDPYNIFWIYET
                     IPTKALKYKLIVKNPITKLKKNQHYFLGDDEFEQLV"
BASE COUNT          454 a          181 c          183 g          358 t
ORIGIN      
        1 ataatttttg atttaattct gcacccaaaa agatacactc ctatctctta tttaaagttg
       61 ctatataccc tcttatttta gcatactccc ttttagaaaa agcaaaaaaa aaagaaaaaa
      121 tcttattctt gataacaagc tacgtgacgt atatgcctaa caaaaataga acttttgaat
      181 caatcttaaa tgaaggttta accacagaac cggcatccat tatttttgat gaagttatcg
      241 atgacgttga agatagtaag tatcataaaa atttaaaaga acttttttat tttgcactca
      301 acaatggacc aagtttgttc actggtatat cagtacctga tagtgataat ccattaatta
      361 tggcaaaaat ttacttaaca aaatggtgta ataaatacat cagggatagg aataacccag
      421 cattaaaaaa acctcttaaa acatttggcg aaaaagatgc tgctttaact actagagtag
      481 cagcaaatgc taatattgat gatcaaaaag tattgaatga ctacttgcgt ggacattttt
      541 tatatatgag cgctgagaac atgaacggaa gtattttaga ggaatattta gccgaagtgt
      601 tagaacctga aggttggatt tggtgcgccg gctctgtata tcgagctgtt gatttctgtt
      661 atttaggaac ctcaccaatt cttttacaag tgaaaaataa atacaataca gaaagcagtt
      721 cgagttcagc tataagagta ggaacaacaa ttagaaaatg gaatcgttta aataagtcga
      781 caaaaatttc tggtttggat agccctatcc ccaattggaa agcattaata gaaatgactg
      841 aagctagtaa ggaactggca gctaagttaa ctgaaaatag ttatttagct tatattaatg
      901 aaaaatccac acgagaatta tggacactcg atgactaaca atatccttat tagtatctat
      961 ccacgataca tggaaaaaat tataaacaaa caaaaaaact ttgaatttag aaatttctat
     1021 cttacctcag atccttataa tatcttttgg atttacgaaa caatccccac caaagcttta
     1081 aagtataaat tgattgtaaa aaatccaatt actaaactta agaaaaatca acactacttt
     1141 ttaggagacg atgagtttga acaattagtt taaaag
//
LOCUS       DACDXO010000167         1167 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E23605_2017
            SAMN12639989-rid9881463.denovo.182, whole genome shotgun sequence.
ACCESSION   DACDXO010000167 DACDXO010000000
VERSION     DACDXO010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639989
            Sequence Read Archive: SRR10023389
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1167)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1167)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741914.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:42:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,972
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 33 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 17 of 73
            Pseudo Genes (multiple problems)  :: 10 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1167
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639989-rid9881463.denovo.182"
                     /isolate="E23605_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..>1167
                     /locus_tag="IWM35_002839"
     CDS             <1..>1167
                     /locus_tag="IWM35_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ISL3-like element ISEfa5 family transposase"
                     /protein_id="HAP9920605.1"
                     /translation="QKKKTLIVDAVFSPAPHTCRNCGSTVVDGNGKVIVVKNGKKETI
                     VRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQSYFVQPRHSIANHVRYKIASLLTEKV
                     SLSFIAKNCQVSLTTVIRTLKEFKSYLPKQSKRILPRVLMVDEFRSHASIEDKMSFIC
                     ADGETGQLIDVLPTRKLPRLTSYFLGCANPEEVKFLVTDMNAAYFQLTKRILPNAKVV
                     IDRFHIIKHMNQAFNELRIREMNELRKVGQKSQAEKLKKNWRFLLKNRANINHYEYKT
                     WKSFRAPKYPFLTEAMMIDRLLGFSTSLKEAYPYFHELVEAFRDKDPDLFFSLLAELP
                     ETLDDGFREKLQNLLTYEEGITNAMIYPYTNGKIEAKNTHIKTMKRVSYGFKSF"
BASE COUNT          383 a          230 c          227 g          327 t
ORIGIN      
        1 ttcagaagaa aaagacgttg atcgtcgatg ctgttttctc acctgctccc catacctgta
       61 gaaattgtgg ttctactgtg gtagatggaa acgggaaagt aattgtagtg aaaaatggga
      121 agaaggaaac gattgtccgc tttgaacaat acaatcacat gcctttggtt atgcgcttaa
      181 aaaagcagcg ctatacctgt aaaaactgtc gaacccattg gacggctcaa agttattttg
      241 ttcaacccag acattcaatc gcgaatcatg tcagatataa aattgcttct ttactgactg
      301 aaaaagtatc tttatctttt attgcgaaaa attgtcaggt atctttgacc accgttattc
      361 gtacgttgaa agagtttaaa agctacttac caaagcaatc taagaggatt ctccccagag
      421 tattgatggt tgatgaattt cgttcgcacg cttccataga agataaaatg agctttattt
      481 gcgcagatgg cgaaacagga caattaattg atgtcttgcc tacgcgtaaa ttgcctcgat
      541 taacaagcta tttcttaggc tgcgctaatc ctgaagaagt aaaattttta gtcacagaca
      601 tgaacgccgc ctacttccag cttaccaaac gtattctgcc aaatgcgaaa gtagtgattg
      661 atcggtttca tattatcaaa cacatgaatc aagcctttaa tgagttgcgt atccgtgaaa
      721 tgaatgaact tcgtaaagta ggacagaaaa gtcaggcaga aaaactgaaa aagaactggc
      781 gctttttgct aaaaaatcgt gcaaacatca accattatga atacaaaaca tggaaaagtt
      841 ttcgagcacc aaaataccca tttcttacgg aagcgatgat gattgatcga ttgcttggct
      901 tttctacgtc cctgaaggag gcgtatccct attttcatga attagttgaa gcctttcgag
      961 acaaagaccc tgacttattt ttctccttat tggcagaact tcccgaaacg ttagatgacg
     1021 gctttcggga aaagctccaa aaccttctga cctatgaaga aggcatcacc aacgcgatga
     1081 tctatcctta caccaatgga aaaatagaag cgaagaatac ccatataaag acaatgaaac
     1141 gagtatccta cggatttaaa tcatttg
//
LOCUS       DACDXO010000168         1143 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E23605_2017
            SAMN12639989-rid9881463.denovo.183, whole genome shotgun sequence.
ACCESSION   DACDXO010000168 DACDXO010000000
VERSION     DACDXO010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639989
            Sequence Read Archive: SRR10023389
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1143)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1143)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741914.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:42:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,972
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 33 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 17 of 73
            Pseudo Genes (multiple problems)  :: 10 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1143
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639989-rid9881463.denovo.183"
                     /isolate="E23605_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(18..923)
                     /gene="xerC"
                     /locus_tag="IWM35_002840"
     CDS             complement(18..923)
                     /gene="xerC"
                     /locus_tag="IWM35_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HAP9920606.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
BASE COUNT          338 a          232 c          194 g          379 t
ORIGIN      
        1 tgttcttttc cttattttta cgctcttgga tggaaagaac gatagttttt ttgtaaactt
       61 tcttttgtta catgggcata gatctgggta gtcgataaat tggcatgtcc tagcaattct
      121 tggactgtcc gcaaatctgc cccattattt aataaatgag tagcaaatgt atggcgcagc
      181 atgtgaggat ggatatcact atccaaactg ctttttttga tcatttgatt caagacatat
      241 tcaattccag taggcgtgat tggttttcca tgatgattca caaaaacgat atcatgctct
      301 tcgtgatatt ttgtcatcaa tacctggcgg ccttccctga aatattcttg cagagcatct
      361 tgagcaaaag aacctagagg agcatatctc tctttgttcc ctttaccatg gatcaacatg
      421 acctctgaat caaaatcaat gtctgatacc gacaaactgc tacactcact taaacggatg
      481 cctgacccgt aaaggacttc cagtaaagcg cggttcctca aatccaaagg actatctcct
      541 gctacgctgt caaaaagtgc ttgcatttca ttttcataaa aaaaacgtgg cagtctcaaa
      601 tttttctttt tgagatgaac gtaagaaaat ggattttctt ttatcacttc ttctttgaga
      661 agatactgat aaaacgaacg taggctggct atcttgcgac tgatagaatt tctgctatag
      721 ctaggacgat gatcagctaa tgcactcaaa tagacacgaa ccgtaaaata ttccgccttc
      781 agcagatcag cgtctccaga ttcctccaaa aaatgtttga aatggagaat gtcttcttca
      841 tatgcttctt tcgttttttc tgaatatcct ctttcgacaa tcaaataacg caaaaatttt
      901 tgttcccacg tggcttctgt cattttctca ccttcctttt gtcccagaga ataatttagc
      961 acagcgttta taaaaagaca aataaattgt cagaatttaa ccaatatttc taagtttgtt
     1021 cacaattggc gcaattcctc acattttgtt tgagaaattc ttaagaaaaa cgcaagaaaa
     1081 agggctctat aatttatggg actgatgaat cagaattgat tcttcagccc cattttcatt
     1141 tta
//
LOCUS       DACDXO010000169         1114 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E23605_2017
            SAMN12639989-rid9881463.denovo.184, whole genome shotgun sequence.
ACCESSION   DACDXO010000169 DACDXO010000000
VERSION     DACDXO010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639989
            Sequence Read Archive: SRR10023389
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741914.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:42:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,972
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 33 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 17 of 73
            Pseudo Genes (multiple problems)  :: 10 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1114
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639989-rid9881463.denovo.184"
                     /isolate="E23605_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..897)
                     /gene="vanY"
                     /locus_tag="IWM35_002841"
     CDS             complement(<1..897)
                     /gene="vanY"
                     /locus_tag="IWM35_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanY-A/VanY-F/VanY-M family D-Ala-D-Ala
                     carboxypeptidase"
                     /protein_id="HAP9920607.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHT"
BASE COUNT          328 a          228 c          159 g          399 t
ORIGIN      
        1 agtatgtgtt gatccgggaa acactgtcac aattacaccg tctatattgt ttcctgatat
       61 ctcataacga agattagtcg gcacatgaat ggtggtattt ttagtaacag gataataaaa
      121 gatctcatat ttttccccat ttacactaac agaaatggtt ttttcttctt ttaggtaatc
      181 catatattcc tcgagaacga aattcttttc tttcataatc gcactatgtg gtaaaccaac
      241 atagcgaata tgccatggtt catattgaat tcctgttaac tctgttttgt cctctggata
      301 acgtaaaatg aacccgtatt tccaagcatt ttcttctatc cactttcctt caggggctcg
      361 ttccattttc gtcaagcttg atcctacatc tagtgataaa cctgaattat gctcactata
      421 acctgctggt aaggcatact cagcccccat ttcttggtaa agcacacttt gctcatcaaa
      481 gtctcgatag ccactattaa taataaaatg actaacgcca ccctttacag catcattgac
      541 catctctgaa aatttttgtg ctatttcttt tgacatataa atattactat caagcaaccc
      601 gtatccattt attaattcgt catgtttaga taaattcacg atatctgact tcacactttc
      661 ttggcgaaca ggatatttac tattgattaa tagcagattt ccttgataaa cctgttcttc
      721 tgtaattgtt ttctcttggg tattttcaga agtcccacta ttttctaaat gttctttggg
      781 attttgatca taattttgaa attcgacttt ttcctgagaa aacagtgctt cattaacgta
      841 gtcataacct aagtatatta agaataacaa taacaataaa aaaaacaact tcttcatttt
      901 cagtctcctc cttaactata tagttggaag tatgaaatat ttctagaatc ttccccttaa
      961 acggcacaat ctcgtttgag attgtgccgt tttgccccgt aaattacagt caattatttc
     1021 gccctactgg atttactatt atcaccaatg tagatgaatt ttcataagtg atacccatgc
     1081 tgaatttaac gaggctgaac tgcctgttga caat
//
LOCUS       DACDXO010000170         1064 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E23605_2017
            SAMN12639989-rid9881463.denovo.185, whole genome shotgun sequence.
ACCESSION   DACDXO010000170 DACDXO010000000
VERSION     DACDXO010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639989
            Sequence Read Archive: SRR10023389
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1064)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1064)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741914.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:42:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,972
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 33 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 17 of 73
            Pseudo Genes (multiple problems)  :: 10 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1064
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639989-rid9881463.denovo.185"
                     /isolate="E23605_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            81..782
                     /locus_tag="IWM35_002842"
                     /pseudo
     CDS             81..782
                     /locus_tag="IWM35_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
BASE COUNT          351 a          176 c          181 g          356 t
ORIGIN      
        1 acctccagac ttcttgatag ctcaagtata tcttgaggtg catttgttag taaggtactg
       61 ttctatgggg cgcttacaat tattccgtta tatttatgga tgcgacgcat atccctgtga
      121 agcgacagac tgtttcgaag gaagcagtgt atattacgat tggtattcgt ttggatggaa
      181 ccaaagaagt tcttgggttt actattgccc caactgagtc tgcttatatt tggaaagaag
      241 ttcttcaaga tcttagaaaa cgtgggttag aagaagtttt attagtagtg acagacggat
      301 taagctgtat tgaagaaagt atccatagtg tgtatccgaa tgcccaattt caacaatgtt
      361 gtgtgcatgt atctagaaat atcgctcata aagttcgtgt tcgaggtcga aaagaaattt
      421 gtgaggattt caaattggtt taccaagcga attcaaaaga agaggcattg gatcacatcg
      481 actttatgac taggaaatgg aaaaagcagt atccaagagt cgtcaattta ctcttgaatc
      541 ctgccctatt aaccttttat aatttccctc acgccatcag acgaacaatt tattcgacga
      601 acctgattga aggctttaat aagcagctaa aacgatatac tcgaagaaaa gaacaattcc
      661 ctaatgaaga atctctagag agattcttca tttctcaatt taatcaatat aaccaaaaat
      721 ttttaggtag aattcacaag ggatttaaag aaattcagga tacattagag tcgatgattt
      781 aactgtaatt aacagaatgg attttccatt tacatataat tcttgacgct accataatct
      841 aaataaccat catattcatc tactacttat ataattgagt tttattaaac tttttaagtt
      901 tctattctat caaatattta aacaatctct gcatccgaaa agactacctc gtatagtttt
      961 actaacaata tttttattag cgtgttcaaa tcctgatttc ttgtatcaat tattttctgt
     1021 tcactttcaa taagaaaaaa gtctttactt ccatggataa taat
//
LOCUS       DACDXO010000171         1061 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E23605_2017
            SAMN12639989-rid9881463.denovo.186, whole genome shotgun sequence.
ACCESSION   DACDXO010000171 DACDXO010000000
VERSION     DACDXO010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639989
            Sequence Read Archive: SRR10023389
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741914.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:42:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,972
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 33 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 17 of 73
            Pseudo Genes (multiple problems)  :: 10 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1061
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639989-rid9881463.denovo.186"
                     /isolate="E23605_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(36..992)
                     /locus_tag="IWM35_002843"
     CDS             complement(36..992)
                     /locus_tag="IWM35_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9920608.1"
                     /translation="MELYTLPIIIKTEKMLKDLKQYYPLSAYEKKKLFHFLAINLLAE
                     QADSLDIASNQSLFFSDIGFYHLKGVLGEKSVIFINKTLSWLYLHDKLDQKYLRKNED
                     ETIEWLNERFIESFENQFNLLREETSQLIRRELTKIHFKLLHYTINKADEFPLDVSFF
                     RENYPAFYCYLIEYINDLTSKRKELLQDKFFLFFSYLLLLINHIPIQLISEPVKIIID
                     FSYGAAYNQFIKKNLSLYINLNTEVIEAGKPEFPDVIITNRNNLYEESASQVVVWLDP
                     PRAVDWGNLTKILLTIQEEKYKDSKIDKQIDDFPEEIIDRIE"
BASE COUNT          346 a          177 c          160 g          378 t
ORIGIN      
        1 tagaaaaagc agtcggactt tctaaaagaa gttcactatt cgatcctgtc gatgatttct
       61 tcaggaaaat cgtctatttg cttgtcaatt ttgctgtctt tgtatttctc ttcttggata
      121 gtcaacaaaa tctttgtaag attcccccag tctactgctc ttggcggatc gagccaaacc
      181 actacttgag atgcactttc ttcatataga ttattcctat tcgtgattat aacatccggg
      241 aactcaggct ttcctgcctc tatcacttcc gtgttcagat taatgtacaa gcttaaattt
      301 ttcttgataa attgattata ggcagcacca taagaaaaat cgatgatgat ctttactggt
      361 tcagaaatca gttgaatggg tatgtgattg atcaataata ataaataact gaaaaaaaga
      421 aaaaatttat cttgaagcaa ctctttgcgt tttgacgtta gatcatttat atactctatc
      481 aaatagcaat aaaatgctgg atagttttct ctaaaaaaag aaacatcaag tggaaattca
      541 tcagctttat taatagtgta atgcaaaagt ttaaaatgta ttttggttaa ctctcttcgg
      601 atcaattggc tcgtctcttc tcgcaacaga ttaaactgat tttcaaaact ttcaatgaat
      661 cgttcattca accattcgat tgtttcatct tcattttttc tcaagtattt ttgatccaat
      721 ttatcatgaa gataaagcca agagagtgtc ttattgataa aaataacgga tttttctcct
      781 aatactcctt ttaagtggta aaatcctata tccgaaaaaa atagcgactg attgctagca
      841 atatctaaag aatcagcttg ttcagccaac aggttgattg ctaaaaagtg gaataatttc
      901 tttttttcat aagcagacag aggataatac tgttttaaat cttttaacat tttttctgtt
      961 tttataatta taggtagcgt atacaattcc acaaaactta tctctgttga gttaaaaaga
     1021 ttatataaaa aaagccgaat gtttttagga ttccctgtca a
//
LOCUS       DACDXO010000172         1051 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E23605_2017
            SAMN12639989-rid9881463.denovo.187, whole genome shotgun sequence.
ACCESSION   DACDXO010000172 DACDXO010000000
VERSION     DACDXO010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639989
            Sequence Read Archive: SRR10023389
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1051)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741914.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:42:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,972
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 33 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 17 of 73
            Pseudo Genes (multiple problems)  :: 10 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1051
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639989-rid9881463.denovo.187"
                     /isolate="E23605_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            6..980
                     /locus_tag="IWM35_002844"
     CDS             6..980
                     /locus_tag="IWM35_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase family protein"
                     /protein_id="HAP9920609.1"
                     /translation="MCTSITYVTSDHYFGRNFDYEISYNEVVTVTPRNYKLNFRKVND
                     LDTHYAMIGIAAGIADYPLYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFI
                     PWILGQCSTVGEAKKLLKNINLANINYSDELPLSPLHWLLADKEKSIVIESMKDGLHI
                     YDNPVGVLTNNPSFDYQLFNLNNYRVLSSETPKNNFSNQISLNAYSRGMGGIGLPGDL
                     SSVSRFVKATFTKLNSVSGDSESESISQFFHILGSVEQQKGLCDVGDGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAIDMNKEDLDSHKLISYPIIEKQQIKYIN"
BASE COUNT          377 a          132 c          182 g          360 t
ORIGIN      
        1 aaactatgtg tacgtctatt acttatgtaa caagtgatca ttattttgga aggaattttg
       61 attatgaaat atcttacaat gaagtagtta ctgttactcc aagaaattat aagttgaatt
      121 ttcgaaaggt aaatgatttg gatactcatt atgcaatgat tggtattgcc gctggtatag
      181 ctgactaccc tctttattac gatgcgacaa atgaaaaagg attgagtatg gctgggctaa
      241 atttttctgg gtatgctgat tataaagaaa tacaagaagg gaaagacaat gtatctcctt
      301 ttgaatttat tccttggatt ttaggacaat gctcaacagt aggagaagct aaaaaattgt
      361 taaaaaatat caatttagca aatataaatt atagtgacga acttccttta tcccctttac
      421 attggctatt agctgataaa gaaaaatcaa ttgtcattga aagtatgaaa gatggacttc
      481 atatatatga taaccctgtg ggcgttctta ccaataatcc ttcatttgac tatcaattat
      541 ttaatttaaa caattatcgt gtcttatcga gtgaaactcc taaaaataat ttttcaaatc
      601 aaataagttt gaatgcctat agccgcggta tgggagggat aggcttgcct ggagatttat
      661 cctcagtatc tcgttttgtt aaagcgactt ttacgaagct gaattctgta tctggagatt
      721 cagagtcaga aagtattagt caatttttcc atatcttagg ttcagtagaa caacaaaaag
      781 gtttgtgtga tgtaggtgat ggaaaatatg aatatacaat ttattcttct tgttgcaatg
      841 ttgacaaagg aatctattat tatcgaacat atgaagacag tcaaattact gcaattgata
      901 tgaataaaga agacttagat agtcataagt taattagtta tccaattata gaaaaacaac
      961 aaattaaata tataaattag ttaatgtgtt gtgattgatt atttaatata gatataatac
     1021 aaaaaggcaa agatattttc aaaacatttt t
//
LOCUS       DACDXO010000173          994 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E23605_2017
            SAMN12639989-rid9881463.denovo.188, whole genome shotgun sequence.
ACCESSION   DACDXO010000173 DACDXO010000000
VERSION     DACDXO010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639989
            Sequence Read Archive: SRR10023389
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741914.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:42:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,972
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 33 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 17 of 73
            Pseudo Genes (multiple problems)  :: 10 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..994
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639989-rid9881463.denovo.188"
                     /isolate="E23605_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(25..912)
                     /locus_tag="IWM35_002845"
     CDS             complement(25..912)
                     /locus_tag="IWM35_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP9920610.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLIGTSLLVGPMMVTAVP
                     QHVHADEQGSQTVADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLAS
                     APNYNLFGVKGSYNGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTS
                     FSTGDYHYAGVWKSNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTG
                     SATTTTSTTTTTTTTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMA
                     KNGMSMSSYSLLVGQELNV"
BASE COUNT          288 a          207 c          179 g          320 t
ORIGIN      
        1 aatttctttg cctttttatc taaatcaaac gtttagttct tgaccaacta ataaagaata
       61 actggacata gacatgccat ttttcgccat tagatcatca acagacatgc caaatttttg
      121 tgcaatatcc catagcgtat cacctgacac gactgtatat gtctgaccat tatttgtatt
      181 tacatctgtt gttgaagatg atgacgttga agtcgtcgta gtagtcgttg ttgttgtact
      241 tgtggttgtt gtagccgaac ctgttgatgg cgtatcaaaa cgggttaaat tataagtatt
      301 aatcaatgag ttcaatttgg ccccataatt tggatcggta gcataacgac ctgtcaagta
      361 cgctgttgca tcataaaaac ttgttgtatt gcttttccac acgccagcat aatgataatc
      421 gcccgtggaa aaactggttg aacgaagaac attcgcatgg tcttggaagg attcccaata
      481 agaagaatat tttcggaatg gctcattcat cgttacccat tgtccgttta catattcttt
      541 ggttgccata taaacgacct gtccgttata gcttcctttc acaccgaata gattataatt
      601 tggcgcacta gctaatccgg aagtgccata tgcactttcc aacaacgctt gagcgatcat
      661 gactgaagca tacaagtcat tacttgcagc aacttctgac gcagaccagc cgatttgatc
      721 gataaacgct tgaggatcag cgactgtttg agatccttgt tcatctgcat gaacatgttg
      781 cggtaccgct gtaaccatca tcggcccaac taaaagactc gttccaatta aaggagcaat
      841 cgctttttta ttgatcacca tttttctttt aggtctatgg tgccgggcac tgcgggtctt
      901 cagctcttgc attcaaattc ctcctcaaaa taaaaaaact taataaaaag ttctcttctt
      961 aaaacaaact taattaatta aattatactt ttaa
//
LOCUS       DACDXO010000174          994 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E23605_2017
            SAMN12639989-rid9881463.denovo.189, whole genome shotgun sequence.
ACCESSION   DACDXO010000174 DACDXO010000000
VERSION     DACDXO010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639989
            Sequence Read Archive: SRR10023389
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741914.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:42:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,972
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 33 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 17 of 73
            Pseudo Genes (multiple problems)  :: 10 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..994
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639989-rid9881463.denovo.189"
                     /isolate="E23605_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..>994)
                     /locus_tag="IWM35_002846"
     rRNA            complement(<1..>994)
                     /locus_tag="IWM35_002846"
                     /product="16S ribosomal RNA"
BASE COUNT          209 a          307 c          225 g          253 t
ORIGIN      
        1 agcacgtgtg tagcccaggt cataaggggc atgatgattt gacgtcatcc ccaccttcct
       61 ccggtttgtc accggcagtc ttgctagagt gcccaactga atgatggcaa ctaacaataa
      121 gggttgcgct cgttgcggga cttaacccaa catctcacga cacgagctga cgacaaccat
      181 gcaccacctg tcactttgcc cccgaagggg aagctctatc tctagagtgg tcaaaggatg
      241 tcaagacctg gtaaggttct tcgcgttgct tcgaattaaa ccacatgctc caccgcttgt
      301 gcgggccccc gtcaattcct ttgagtttca accttgcggt cgtactcccc aggcggagtg
      361 cttaatgcgt tagctgcagc actgaagggc ggaaaccctc caacacttag cactcatcgt
      421 ttacggcgtg gactaccagg gtatctaatc ctgtttgctc cccacgcttt cgagcctcag
      481 cgtcagttac agaccagaga gccgccttcg ccactggtgt tcctccatat atctacgcat
      541 ttcaccgcta cacatggaat tccactctcc tcttctgcac tcaagtctcc cagtttccaa
      601 tgaccctccc cggttgagcc gggggctttc acatcagact taagaaaccg cctgcgctcg
      661 ctttacgccc aataaatccg gacaacgctt gccacctacg tattaccgcg gctgctggca
      721 cgtagttagc cgtggctttc tggttagata ccgtcaaggg atgaacagtt actctcatcc
      781 ttgttcttct ctaacaacag agttttacga tccgaaaacc ttcttcactc acgcggcgtt
      841 gctcggtcag actttcgtcc attgccgaag attccctact gctgcctccc gtaggagttt
      901 gggccgtgtc tcagtcccaa tgtggccgat caccctctca ggtcggctat gcatcgtggc
      961 cttggtgagc cgttacctca ccaactagct aatg
//
LOCUS       DACDXO010000175          960 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E23605_2017
            SAMN12639989-rid9881463.denovo.190, whole genome shotgun sequence.
ACCESSION   DACDXO010000175 DACDXO010000000
VERSION     DACDXO010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639989
            Sequence Read Archive: SRR10023389
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 960)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 960)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741914.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:42:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,972
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 33 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 17 of 73
            Pseudo Genes (multiple problems)  :: 10 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..960
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639989-rid9881463.denovo.190"
                     /isolate="E23605_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            376..>960
                     /locus_tag="IWM35_002847"
     CDS             376..>960
                     /locus_tag="IWM35_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HAP9920611.1"
                     /translation="MSKRGTIVNEWEKLNRISNRRLKYQSLTLPLKMQWSIDRITIVG
                     KLKENIYYHTPNDVLILNFEQLMRLNEGNGYLKAVGNNGWQLLDQHEENIAYIEILKF
                     HEGKGRIDFNPNKINQFLSGSMKNFIHDLFLEPHFSRADIACDIVDIPDDFVSQYRVV
                     DPVSFKPIYGRSGKLETAYWGSRASERQIRMYNKK"
BASE COUNT          310 a          149 c          190 g          311 t
ORIGIN      
        1 cctattaagc tgacatttgc taaaaaatgg ccagtccatt ttttggccaa cctagcgtta
       61 gcgaagtttg ggggcgccct tttcaaaggg tgcggtttta ttttttggat atttgcatag
      121 ctaaagagaa gtgctattat tgagttagca aacaaatgaa ctgaaaaaag agtaaaaaaa
      181 tagtcgagca acgctaccaa cgttctcgac ctttacggaa actaatcgca ctagctccgt
      241 aacttcttaa tcattatacg aatattttta gatttcttca agactagaga ttttttggta
      301 atgaattttt aagagttacg tgaacttttg aaaattcttt ccagaagtct ctttttcttt
      361 gaggaggaac aaaatttgag taaacgtggc acgatcgtga atgagtggga aaaacttaac
      421 cgcatttcta acaggcggtt aaagtatcaa agtttgaccc ttcccttaaa aatgcagtgg
      481 tcaattgacc gaattaccat agtcggaaaa ctgaaagaaa atatttatta tcacactcca
      541 aatgatgtgt tgattttaaa ttttgaacag cttatgcgtt tgaacgaagg caatggttat
      601 ttaaaagctg ttgggaataa tgggtggcaa ttattagacc aacacgagga aaatatagct
      661 tatattgaga ttcttaaatt tcatgaagga aaagggcgga ttgactttaa tcctaataaa
      721 atcaatcaat ttttatcagg ttccatgaaa aatttcattc atgatttatt tctagaacct
      781 catttttcac gtgcggatat tgcttgcgat attgtagata ttcccgatga ctttgttagt
      841 cagtatcgtg tagttgatcc tgtgtctttt aaaccaattt atgggcgttc tggtaagtta
      901 gaaacggcat attggggtag tcgtgccagt gaacgacaaa ttcgaatgta caataaaaaa
//
LOCUS       DACDXO010000176          931 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E23605_2017
            SAMN12639989-rid9881463.denovo.191, whole genome shotgun sequence.
ACCESSION   DACDXO010000176 DACDXO010000000
VERSION     DACDXO010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639989
            Sequence Read Archive: SRR10023389
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741914.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:42:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,972
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 33 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 17 of 73
            Pseudo Genes (multiple problems)  :: 10 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..931
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639989-rid9881463.denovo.191"
                     /isolate="E23605_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..>931
                     /locus_tag="IWM35_002848"
     CDS             <1..>931
                     /locus_tag="IWM35_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAP9920612.1"
                     /translation="HCFISQKVRWSVIARLKENTSMTEIARQKNISTSSVYRVMKRFY
                     RPLNPFKQTLPKVLCFDEFKSVRSVASAMSFIMMDGQSHALLDIVENRRLPYLERYFS
                     RFSLATREAVQLIVIDMYAPYVSLVKKLFPNAQLIIDRFHIVQHIGRTFLNHRVKETT
                     VRLKGNSHSQRGLGKKLKRYWKLLQKEEAKLNYEKRVWRASFKDYLTESEIADRLLAA
                     CPNLRQGYQLYQDILYAVKKRDQSLFEDCLTRDISGLPETYTTTLRTFKKFLPQIQNA
                     LHYSYSNGPLECLNNHIKVLKRNAYGFRNFYNFK"
BASE COUNT          286 a          190 c          180 g          275 t
ORIGIN      
        1 acactgtttc attagtcaaa aagttcgttg gtctgtcatt gctcgcttaa aagagaatac
       61 ttccatgacg gaaatcgctc gtcagaaaaa tatttccact tcttcggtct atcgtgtcat
      121 gaagcgattc taccgcccat tgaatccttt caaacagact ttgccaaagg tcttatgctt
      181 tgatgaattc aaatcggttc gaagtgttgc cagtgcaatg agttttatca tgatggacgg
      241 acaaagccat gcattattgg atattgtcga aaatcgacgc ttgccttatt tagaacgcta
      301 tttttctcgg ttttcattag cgactcgtga agccgttcaa ttgattgtta ttgatatgta
      361 tgcgccttat gtttcgttgg tgaaaaagct tttcccgaat gctcagttga tcatcgatcg
      421 tttccacatc gtccaacata ttggcagaac tttcttgaat catcgagtga aagaaaccac
      481 ggtacgactt aaaggaaatt cccactctca acgagggtta gggaaaaaat tgaagcgcta
      541 ttggaaactc ctacaaaaag aagaagcaaa gttaaactat gaaaaacggg tttggcgtgc
      601 cagttttaaa gattatttga ctgaaagtga gattgccgat cgactgttag ctgcttgtcc
      661 caacttacgc caaggctatc agttatacca agacattctt tatgcggtaa agaaaagaga
      721 tcagtcacta tttgaggatt gtttaactag agacattagc ggtctgccag aaacctatac
      781 aaccacttta cgaacgttta aaaagtttct gccacagatc caaaatgcgc ttcactacag
      841 ctattcaaac ggaccattgg aatgcttaaa caaccacatc aaagtattaa aacgcaatgc
      901 gtacggcttt cgcaactttt acaatttcaa a
//
LOCUS       DACDXO010000177          915 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E23605_2017
            SAMN12639989-rid9881463.denovo.192, whole genome shotgun sequence.
ACCESSION   DACDXO010000177 DACDXO010000000
VERSION     DACDXO010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639989
            Sequence Read Archive: SRR10023389
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 915)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 915)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741914.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:42:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,972
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 33 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 17 of 73
            Pseudo Genes (multiple problems)  :: 10 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..915
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639989-rid9881463.denovo.192"
                     /isolate="E23605_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(243..464)
                     /locus_tag="IWM35_002849"
     CDS             complement(243..464)
                     /locus_tag="IWM35_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9920613.1"
                     /translation="MEEEKIFEKRWELASVEQRARYHNLMSSYRNIDWTYKEKKYLLW
                     LCQLDVNTFETFEVILDKIKNSNEKRADL"
     gene            complement(562..807)
                     /locus_tag="IWM35_002850"
     CDS             complement(562..807)
                     /locus_tag="IWM35_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9920614.1"
                     /translation="MDFPEVVKEVQEIGATISDSRSSPMNYQDAHGIKIIDQERNVRK
                     YTTINECVINENLCRAPITKHIKNRSKAKDGRTFTTF"
BASE COUNT          274 a          178 c          113 g          350 t
ORIGIN      
        1 actatatact tcttcccaga acttgtccgt ctccttcaaa ttatctctac ctctcgagtt
       61 cattgggtgg tttgcccatt tattaaacat ggaatcacct ttctcatact catatattta
      121 tgtcctattt caattatatt tatatatgct taaataaatt tgtatcctga aatttattgt
      181 aatatgaagc tgtattttac atatagtaac caaagagtta ataaaataaa aagagcgaac
      241 cattatagat cagctctttt ttcattgctg tttttaatct tatctagaat cacttcaaat
      301 gtttcaaagg tatttacatc taactgacat aaccaaagca agtacttctt ttctttgtaa
      361 gtccaatcaa tattacggta agaggacatt agattatggt accttgctct ttgttcaact
      421 gaagccaatt cccagcgttt ttcaaatatt ttttcttctt ccattattga tccctcgcaa
      481 gacgctacct caaacttaaa taatgggcag cgaaagtcta attccttaga tctaagtata
      541 gcaaacaaat atgaaaaaaa cttagaaggt agtaaaagtt cttccgtctt ttgctttgct
      601 tctattctta atgtgtttcg ttatcggtgc cctacaaaga ttttcattaa ttacacactc
      661 atttattgtt gtgtattttc tgacatttct ctcttgatca attattttaa ttccatgggc
      721 atcctgatag ttcataggag aggagcggct atcagaaata gttgcaccta tctcttgaac
      781 ttcttttaca acttctggaa aatccatcca tcctgcttca gctgtcaaaa gtaataatct
      841 ataatattta tctttccttt tcaatctaag aaatcgtcac cccattcttt ttcttctatt
      901 ataaactatt taact
//
LOCUS       DACDXO010000178          897 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E23605_2017
            SAMN12639989-rid9881463.denovo.193, whole genome shotgun sequence.
ACCESSION   DACDXO010000178 DACDXO010000000
VERSION     DACDXO010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639989
            Sequence Read Archive: SRR10023389
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 897)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 897)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741914.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:42:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,972
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 33 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 17 of 73
            Pseudo Genes (multiple problems)  :: 10 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..897
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639989-rid9881463.denovo.193"
                     /isolate="E23605_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(91..>897)
                     /locus_tag="IWM35_002851"
     CDS             complement(91..>897)
                     /locus_tag="IWM35_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEfa8 family transposase"
                     /protein_id="HAP9920615.1"
                     /translation="TYYYQAKPIENESDLEEIVQEEFIRNRKAYGTRKLKKCLAKRGL
                     QLSRRRIGRIMKRRGLTSTYTIAHFKGQRTACNEAKTANVLDRTFTQEQPLEAIVTDL
                     TYVRVGKKWHYICLILDLFNREIIGYSCGEKKDASLVKEAFGRIPYSLTDVKLFHTDR
                     GKEFDNQTIHEILNGFGITRSLSRKGCPYDNAVVESTYKSVKVEFVHQYQFETLAQLR
                     LELFDYVHWWNYLRLHGTLAYETPIQIRQQRLAKRILDNERGSDTSGEAA"
BASE COUNT          236 a          211 c          173 g          277 t
ORIGIN      
        1 ttgtgaatgc tgcggtgtta tggctacgat tgcccttgac aatgagcatc ctcggatctg
       61 attttctccg gcagaagcac aatcattcaa ttacgctgcc tctccagagg tatcagatcc
      121 gcgctcatta tcaaggattc gcttcgccaa tctctgttgt cgaatttgga tcggtgtttc
      181 atacgccaac gtgccatgta agcgtagata gttccaccaa tgcacataat caaacaattc
      241 tagacgtagc tgtgccagtg tctcaaattg gtattgatgc acgaattcta ctttgacaga
      301 tttataggtt gattccacaa cggcattatc atacggacaa cccttcctac tcaatgaacg
      361 agtaattcca aaaccattca gaatctcatg aatggtttgg ttatcaaatt cctttccccg
      421 gtctgtatga aaaagcttga cgtctgttaa agaatacggt atccgtccaa aggcttcttt
      481 taccaatgag gcatctttct tctcaccaca ggaataacca ataatttctc gattaaacaa
      541 atcaagtatt aagcagatat aatgccactt tttccccacg cgaacataag taagatccgt
      601 aacgatggct tccaatggct gttcttgtgt aaaggtccga tctaatacat tcgctgtttt
      661 cgcttcatta caagctgttc gttgcccttt aaaatgagcg atcgtatagg tagatgtcaa
      721 tccgcggcgt ttcatgattc gaccgattcg gcgccgactg agttgaagcc cacgctttgc
      781 taaacacttc ttcaattttc gggtaccgta agcctttcgg tttcgaataa actcttcctg
      841 aacgatctct tctaagtcgg actcattttc gatcggtttc gcttgataat agtaggt
//
LOCUS       DACDXO010000179          844 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E23605_2017
            SAMN12639989-rid9881463.denovo.194, whole genome shotgun sequence.
ACCESSION   DACDXO010000179 DACDXO010000000
VERSION     DACDXO010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639989
            Sequence Read Archive: SRR10023389
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 844)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 844)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741914.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:42:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,972
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 33 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 17 of 73
            Pseudo Genes (multiple problems)  :: 10 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..844
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639989-rid9881463.denovo.194"
                     /isolate="E23605_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            13..>844
                     /locus_tag="IWM35_002852"
     CDS             13..>844
                     /locus_tag="IWM35_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP9920616.1"
                     /translation="MTYTHLTTDELVIIESYFKMNQSVAKTAHCLNRSRQTIHKVYLF
                     FKQGKSALEYYQQYKKNKSNCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPIS
                     CSARTIYRMFKKGLFDSSDLPMKGKRKPNGHQERRGKQTFRRSIHEREKDYSQFSNEF
                     GHLEGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAIDIEKKLNQWFESVPKNLFK
                     SITFDCGKEFSNWKQISNVNDIAIYFADPGTPSQRGLNENSNGLLRRDGLLKSMDFNS
                     V"
BASE COUNT          289 a          147 c          163 g          245 t
ORIGIN      
        1 gagtgagtac aaatgaccta tacccatctt acaacggatg aacttgtaat aatagagtct
       61 tatttcaaaa tgaatcaatc tgttgctaaa actgcccatt gcttgaatcg ttcaagacaa
      121 acgatccata aagtatatct gttcttcaag caaggaaaat cagccttaga atattatcaa
      181 cagtataaga aaaacaaatc aaactgtggt agacgtccgc ttgttttacc cgaggaacaa
      241 tcagaatata ttcaaagaaa ggttgttcaa ggatggacac ccgatgtgat tgttggtcgt
      301 gcagcgtttc ctattagttg ttctgctcgt accatttatc gtatgttcaa aaaggggcta
      361 tttgattctt ctgacttacc gatgaaaggc aagcgtaaac cgaatggaca tcaagaaaga
      421 cgtggaaaac aaactttccg ccgctctatt catgaacgtg aaaaggatta tagccaattc
      481 tcaaatgagt ttggtcacct tgaaggtgac actatcgtag gtctgaaaca taaaagtgct
      541 gtaattacct tagttgaacg attatcaaaa gttatcatca cattgaaacc gtgtggtaga
      601 caagcgattg atattgaaaa aaaattaaat caatggtttg aatctgtacc gaaaaaccta
      661 ttcaaatcca tcacttttga ttgtggaaag gaattttcaa attggaaaca gatcagtaat
      721 gtcaatgata ttgccattta tttcgctgat ccaggaacgc cgtctcaaag aggcctaaac
      781 gagaattcta acggattgtt acgtagagat ggtttattga aatctatgga tttcaattca
      841 gtag
//
LOCUS       DACDXO010000180          836 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E23605_2017
            SAMN12639989-rid9881463.denovo.195, whole genome shotgun sequence.
ACCESSION   DACDXO010000180 DACDXO010000000
VERSION     DACDXO010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639989
            Sequence Read Archive: SRR10023389
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 836)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 836)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741914.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:42:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,972
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 33 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 17 of 73
            Pseudo Genes (multiple problems)  :: 10 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..836
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639989-rid9881463.denovo.195"
                     /isolate="E23605_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..693
                     /locus_tag="IWM35_002853"
     CDS             <1..693
                     /locus_tag="IWM35_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAP9920617.1"
                     /translation="FVLFNQFKIDGKSDIPFAEIQVHEKALPLLNNLEEWVRYSLQQF
                     NELESSYSKTMFRLLKQYRTKGFAYFSKEDFHELLDIPKSYNQGTIDQRVFNPIRQEL
                     TAIFKGLTIKKKYGKGRGKPVIGYQFTFKPEIKKSDDFNKHAPTKEEQNKALELANQQ
                     VQSEKAPTSLQEKIKEQKRKLELLQQLEKLEREQNTQNKENNAQKSEINSDIPSELKK
                     DLLDNLQNLFKD"
BASE COUNT          352 a          137 c          121 g          226 t
ORIGIN      
        1 tttgttttat ttaatcaatt taaaatcgac ggaaaatctg atattccttt tgctgaaata
       61 caagttcatg aaaaagcctt acccttactt aataatcttg aagaatgggt gcgctattct
      121 ctccaacaat tcaatgaatt agaaagcagt tattcaaaaa caatgtttcg attactaaaa
      181 caatacagaa ccaaaggttt cgcttatttt tcaaaagaag actttcatga attacttgat
      241 attcctaaaa gttacaacca aggtacgatt gatcaacgtg tgttcaatcc aatacgacaa
      301 gaactaactg caatctttaa aggtttaaca atcaaaaaga aatacggaaa aggacgggga
      361 aagcctgtca ttggctatca atttacattt aaacctgaga taaaaaaatc agacgacttt
      421 aacaaacatg ccccaacaaa agaagaacaa aacaaagcat tagaactagc taaccaacaa
      481 gttcaatctg aaaaagcacc tacttcatta caagaaaaaa tcaaagaaca aaagcgaaaa
      541 ttggaacttt tacaacagtt agaaaagtta gaaagagaac aaaataccca aaataaagaa
      601 aataatgcac aaaaatcaga gataaattcc gatattcctt cagaactaaa aaaagactta
      661 ttagacaatc ttcaaaactt attcaaagat tgaggggttt ttagatttta tgacagaaaa
      721 aacaatcaaa gacggagaaa aaatggtggc aattctggca ataaaaccag caatttttat
      781 taatttaaat tgctggtttt attcttcttt ttgcttttgc gagcagagca acatgg
//
LOCUS       DACDXO010000181          825 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E23605_2017
            SAMN12639989-rid9881463.denovo.196, whole genome shotgun sequence.
ACCESSION   DACDXO010000181 DACDXO010000000
VERSION     DACDXO010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639989
            Sequence Read Archive: SRR10023389
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 825)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 825)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741914.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:42:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,972
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 33 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 17 of 73
            Pseudo Genes (multiple problems)  :: 10 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..825
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639989-rid9881463.denovo.196"
                     /isolate="E23605_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..216
                     /locus_tag="IWM35_002854"
     CDS             <1..216
                     /locus_tag="IWM35_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9920618.1"
                     /translation="FADFERWLSSISDDQQHEKSVDNEWLADEKSAPSERDYPDESDG
                     LFGVIPNKKSQMSESKFTSIFNDVNLR"
     gene            437..>825
                     /locus_tag="IWM35_002855"
     CDS             437..>825
                     /locus_tag="IWM35_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
                     /protein_id="HAP9920619.1"
                     /translation="MGFSISFKKGTKNTSISHNNRELTDSQKINDWHKHIDFTKSDEN
                     IYLEQTNIREKYEELFGEAVEKYNAKQKRADRKIEDYLAKVRKDKKLEPQREFIVQIG
                     TLDDFRTTRDDGSSTGISEQQAEQNRV"
BASE COUNT          291 a          130 c          183 g          221 t
ORIGIN      
        1 tttgctgatt ttgagcggtg gctatcgtcg atttctgacg atcagcaaca cgaaaaatca
       61 gtggataatg agtggcttgc tgatgaaaaa tcagcgccaa gcgaacgaga ttatcccgat
      121 gaatctgacg gcttattcgg agtgataccg aataagaaaa gtcagatgag tgagagtaaa
      181 tttacgtcaa tatttaatga cgtgaattta cgataagcga ttattttgcg tgaagcaaaa
      241 taaagcgtaa aaggagttga gagcgtgaca cgctttcggc tccttttttc gttgtcactt
      301 gtgacagcga ataccctcgg agagcatttg gaattgtgac agccatgtca caattaggaa
      361 aatgttactc tttgacaaag agtaacagaa aggtttacac tctttttatt agcgacagcc
      421 gaaggagggt tttaacttgg ggttcagtat ttcgttcaaa aagggaacaa agaatacaag
      481 catttctcat aataatcgtg agttaactga tagccaaaaa atcaatgact ggcataagca
      541 tattgatttc acaaaatcgg atgaaaatat ctatttagaa caaacaaata ttcgtgaaaa
      601 atatgaagaa ttattcggtg aagccgtaga aaaatataat gcaaagcaga aaagagccga
      661 tagaaaaatc gaggactatt tagcaaaggt tcgcaaggat aaaaaattgg aaccacagcg
      721 agaatttatc gtgcaaattg gtactcttga tgattttcgt actactcgtg atgatggttc
      781 ttctactggc atatcggaac aacaagcaga acaaaatcga gtgat
//
LOCUS       DACDXO010000182          811 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E23605_2017
            SAMN12639989-rid9881463.denovo.197, whole genome shotgun sequence.
ACCESSION   DACDXO010000182 DACDXO010000000
VERSION     DACDXO010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639989
            Sequence Read Archive: SRR10023389
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741914.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:42:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,972
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 33 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 17 of 73
            Pseudo Genes (multiple problems)  :: 10 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..811
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639989-rid9881463.denovo.197"
                     /isolate="E23605_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..672
                     /gene="tnpB"
                     /locus_tag="IWM35_002856"
     CDS             <1..672
                     /gene="tnpB"
                     /locus_tag="IWM35_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family element transposase accessory
                     protein TnpB"
                     /protein_id="HAP9920620.1"
                     /translation="SGKYYISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDN
                     NKFTSKMEKKLKREQRKLSRRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTD
                     FLNKLSTEMIKNHDIICIEDLNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRK
                     IIKVDKWFPSSQICSECGHKDGKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLH
                     SMGLA"
BASE COUNT          298 a          126 c          152 g          235 t
ORIGIN      
        1 agcggtaaat actatatttc tttgttgtgt aaagaagaaa ttatcgaatt acctaaaact
       61 aattccacaa ttggtattga cttaggtatt atggactttg cgattctttc tgatggacaa
      121 aaaattgata ataataaatt cacgtccaaa atggaaaaga aactaaagcg tgaacaacgc
      181 aaattgtcta ggcgtgcttt attggctaaa aataaaggta ccaatctatt tgaagctaaa
      241 aattatcaaa aacaaaaacg caaagtagct agattgcatg aaaaagtaat gaatcaacgt
      301 actgattttc tgaataagtt gagtacagaa atgatcaaaa atcacgatat tatctgtatt
      361 gaagacttaa acacaaaagg tatgttgcga aatcataaat tagctaaaag tatttctgat
      421 gtttcttggt caaagtttgt gactaaatta caatacaagg ctgactggta tggacgaaaa
      481 atcatcaaag tagataaatg gtttccgtct agtcagattt gttcagagtg cggacataaa
      541 gatggcaaga aatctcttga aattcgagaa tggacttgtc ctgtttgtca tactcatcat
      601 gatcgtgata tcaatgctag tatcaatatt ctgaccgaag gtctaagact acattctatg
      661 ggattggctt agatccaatc atcaagaacc gtaggaacta cggggatagc ttggtaaata
      721 agagacaccg ctgttagtaa agaaatacgc tatcaagtat gctctattcc caagaagctc
      781 ccacttcaag cgttaagaac cgtcaggttt a
//
LOCUS       DACDXO010000183          765 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E23605_2017
            SAMN12639989-rid9881463.denovo.198, whole genome shotgun sequence.
ACCESSION   DACDXO010000183 DACDXO010000000
VERSION     DACDXO010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639989
            Sequence Read Archive: SRR10023389
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 765)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 765)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741914.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:42:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,972
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 33 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 17 of 73
            Pseudo Genes (multiple problems)  :: 10 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..765
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639989-rid9881463.denovo.198"
                     /isolate="E23605_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..276)
                     /locus_tag="IWM35_002857"
     CDS             complement(<1..276)
                     /locus_tag="IWM35_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9920621.1"
                     /translation="MTYKHLTIDELTMIESYYLQHNKPVEIANRMGRAIQTIYNVVNK
                     FKQGKTALDYWHQYKENKKKCGRKVIQLPAHEVDYIKEKVTLGWTPDV"
BASE COUNT          259 a          129 c          128 g          249 t
ORIGIN      
        1 gacgtcaggc gtccaaccaa gagtgacttt ctctttaatg taatctactt catgagcagg
       61 taattgaatg acttttctac cacatttttt cttattttct ttatactggt gccaataatc
      121 aagagcagtc ttgccttgct tgaacttatt gactacatta taaatagttt gtatagcacg
      181 tcccattcgg ttagcgattt caaccggctt attatgttga agataatatg attctatcat
      241 tgtcagttcg tctatggtaa gatgtttgta ggtcatttat ggttacactc ctttgttttc
      301 tttcgtcgga aatacaattt gagtgtacca taaatgcctt tttatttttc taacttaatt
      361 ttacaattcg cgatatatta aacaacgttg tcgcgcttca ttgaaacatt ttatgaaaat
      421 tccaaaaaag agttgacaca tgtttttaga cgtgataaga taactaagtc gttaaaaaga
      481 accacttgcg aagcttgatt ctatcgaaaa aaaacttcaa aaaagttctt gacaaataac
      541 aaacgataag ttatgataaa tgagttgctg aaagatttcg aaaaacgaaa tcgaaaaaaa
      601 cttttaaaaa agagttgaca atcgcttgtc tctctgatat gatataaaag ttgctacggc
      661 agccaaaaac gaatcgggaa acactcccga tgaagtagac ctttgaaaac tgaacaaagt
      721 aagacaaacc aaatgtgtag ggcgtcttga ttcaattcaa gacaa
//
LOCUS       DACDXO010000184          751 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E23605_2017
            SAMN12639989-rid9881463.denovo.199, whole genome shotgun sequence.
ACCESSION   DACDXO010000184 DACDXO010000000
VERSION     DACDXO010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639989
            Sequence Read Archive: SRR10023389
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 751)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 751)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741914.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:42:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,972
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 33 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 17 of 73
            Pseudo Genes (multiple problems)  :: 10 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..751
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639989-rid9881463.denovo.199"
                     /isolate="E23605_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(77..436)
                     /locus_tag="IWM35_002858"
                     /pseudo
     CDS             complement(77..436)
                     /locus_tag="IWM35_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          215 a          138 c          153 g          245 t
ORIGIN      
        1 tgtagtgacc cccaaaagtt agactttttt ggagtggaga aatccactct ttttttcatg
       61 ctctaaaatg atatttttac acaaccgtaa cggttgtttt acgaaattgg acgggacttt
      121 gccagtttaa tttctgtttt atccgttcat tattgtagta atagatatag ctatcgatgg
      181 ctgattttaa ttcctcgaaa cagttataaa ctttcccgtg aaaaatctct tgttttaata
      241 agccaaaaaa attctccata agtgaattat ctaagcagtt ccctttccga gacatgcttt
      301 gaaagatttt gtactccttc aatttgtttc tataagcttt catttgatac gaccaacctt
      361 gatctgagtg aaacgtacga cgatagggac aatcgttagt ggtctggata gcttcctcca
      421 aaccttccat taccgcacgt gtttctgaat ggtggtagcg accgatttga acggctaggg
      481 cggaaatacg gtgcaaatgc acggtggttt catgcaatgc ttacagggcg tattttgtaa
      541 gggtatgtaa cggagataat cccgacggta atggatgtga gaggccaagc cgacgctgac
      601 ggtagggcaa acaaacatag ttgcactcta gcgatcgtta tttattttta acggtcaaac
      661 tagggtacaa ctatgcttaa aacacttcgc ctcaacttct ggtagcaaaa gtgattttga
      721 aagtcaaaat attttttatg agttatttat a
//
LOCUS       DACDXO010000185          733 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E23605_2017
            SAMN12639989-rid9881463.denovo.200, whole genome shotgun sequence.
ACCESSION   DACDXO010000185 DACDXO010000000
VERSION     DACDXO010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639989
            Sequence Read Archive: SRR10023389
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741914.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:42:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,972
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 33 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 17 of 73
            Pseudo Genes (multiple problems)  :: 10 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..733
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639989-rid9881463.denovo.200"
                     /isolate="E23605_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            2..>733
                     /locus_tag="IWM35_002859"
     rRNA            2..>733
                     /locus_tag="IWM35_002859"
                     /product="23S ribosomal RNA"
BASE COUNT          226 a          146 c          216 g          145 t
ORIGIN      
        1 attgtgtgaa tacatagcac attcgaggta gacgcagaga actgaaacat ctaagtacct
       61 gcaggaagag aaagaaaatt cgattccctg agtagcggcg agcgaaacgg gaaaagccca
      121 aaccagcaag cttgcttgtt ggggttgtag gactccaata tggtagttct ttcagatagt
      181 cgaatgactt ggaaaagtca gtcaaagagg gtaaaaaccc cgtagacgaa atgtggaaga
      241 cacctaggag gatcctgagt acggcggaac acgagaaatt ccgtcggaat ccgggaggac
      301 catctcccaa ggctaaatac tccctagtga ccgatagtga accagtaccg tgagggaaag
      361 gtgaaaagca ccccggaagg ggagtgaaat agaacctgaa accgtgtgcc tacaacaagt
      421 caaagcccgt taatgggtga tggcgtgcct tttgtagaat gaaccggcga gttacgattg
      481 catgcgaggt taagttgaag agacggagcc gcagcgaaag cgagtctgaa tagggcgttt
      541 gagtatgtag tcgtagaccc gaaaccatgt gatctaccca tgtccaggtt gaaggtgcgg
      601 taaaacgcac tggaggaccg aacccacgta cgttgaaaag tgcggggatg aggtgtgggt
      661 agcggagaaa ttccaaacga acttggagat agctggttct ctccgaaata gctttagggc
      721 tagcctcgga att
//
LOCUS       DACDXO010000186          712 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E23605_2017
            SAMN12639989-rid9881463.denovo.201, whole genome shotgun sequence.
ACCESSION   DACDXO010000186 DACDXO010000000
VERSION     DACDXO010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639989
            Sequence Read Archive: SRR10023389
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741914.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:42:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,972
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 33 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 17 of 73
            Pseudo Genes (multiple problems)  :: 10 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..712
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639989-rid9881463.denovo.201"
                     /isolate="E23605_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..446)
                     /locus_tag="IWM35_002860"
     CDS             complement(<1..446)
                     /locus_tag="IWM35_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAP9920622.1"
                     /translation="MNYEFFLSKEDHRSYSLLKYLEASSNLSESISNVQEELSLSTFL
                     LKKTIDKLKHDLQKFDLEKNLRVTVSELDICLEINGKCSSKALLSRYITDSLSMKMVL
                     AFFQEKYISVENFAKQHHVSYSVAYKVLQRLKRNLKKYQIFFEKRK"
BASE COUNT          216 a          123 c           95 g          278 t
ORIGIN      
        1 aactttctct tttcaaaaaa aatttgatat ttttttagat ttctttttag tctttgtaat
       61 actttataag ccacagaata actcacgtga tgctgtttag caaaattttc tactgagata
      121 tatttttcct gaaagaaagc caataccatt ttcatagata aagaatccgt tatataacga
      181 gataacagag ctttgctaga acattttcca ttaatttcca gacatatatc caattcactg
      241 acggttactc gcaaattttt ctctagatcg aacttttgta aatcatgttt cagcttatcg
      301 atcgtttttt tcaagagaaa tgtcgatagc gacaactctt cttgaacatt cgatattgac
      361 tctgataaat ttgagcttgc ttctaaatat ttaagtaatg aataacttct gtgatcttct
      421 ttgcttaaaa aaaactcata attcattagt catcagctct ttctacgtat attactttcc
      481 atgcattcac caactagctg gggataaaac tgatttcctg gatccgagtc actgttgtca
      541 gcgattcttg tttgtataat atacatcgtc ctgtcttttt tttaacattt tggagagcgt
      601 tttatttggc ctttctcgcg atcaaacctg ttattagaat tatatcattt actagtatgc
      661 atttttatta ataaaatggt atattttata ttgttataaa aagagcccaa cc
//
LOCUS       DACDXO010000187          646 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E23605_2017
            SAMN12639989-rid9881463.denovo.202, whole genome shotgun sequence.
ACCESSION   DACDXO010000187 DACDXO010000000
VERSION     DACDXO010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639989
            Sequence Read Archive: SRR10023389
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741914.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:42:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,972
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 33 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 17 of 73
            Pseudo Genes (multiple problems)  :: 10 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..646
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639989-rid9881463.denovo.202"
                     /isolate="E23605_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..>646
                     /locus_tag="IWM35_002861"
     CDS             <1..>646
                     /locus_tag="IWM35_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019117172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein"
                     /protein_id="HAP9920623.1"
                     /translation="SGKLLEALELEQEESIRFHNCSLFPVYHGSAKNNIGIDNLIEVI
                     TNKFYSSTHRGQSELCGKVFKIEYSEKRQRLAYIRLYSGVLHLRDSVRISEKEKIKIT
                     EMYTSINGELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLPQRKKIENPHPLLQTTV
                     EPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLGKVQMEVISALLQE"
BASE COUNT          232 a           96 c          129 g          189 t
ORIGIN      
        1 gtctgggaaa ttattggaag cattagaact cgaacaagag gaaagcataa gatttcataa
       61 ttgttccctg ttccctgttt atcacggaag tgcaaaaaac aatataggga ttgataacct
      121 tatagaagtg attacgaata aattttattc atcaacacat cgaggtcagt ctgaactttg
      181 cggaaaagtt ttcaaaattg agtattcgga aaaaagacag cgtcttgcat atatacgtct
      241 ttatagtggc gtactgcatt tgcgagattc ggttagaata tcggaaaagg aaaaaataaa
      301 aattacagaa atgtatactt caataaatgg tgaattatgt aaaatcgata aggcttattc
      361 cggggaaatt gttattttgc aaaatgagtt tttgaagtta aatagtgttc ttggagatac
      421 aaaactattg ccacagagaa aaaagattga aaatccgcac cctctactac aaacaactgt
      481 tgaaccgagt aaacctgaac agagagaaat gttgcttgat gcccttttgg aaatctcaga
      541 tagtgatccg cttctacgat attacgtgga ttctacgaca catgaaatta tactttcttt
      601 cttagggaaa gtacaaatgg aagtgattag tgcactgttg caagaa
//
LOCUS       DACDXO010000188          627 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E23605_2017
            SAMN12639989-rid9881463.denovo.203, whole genome shotgun sequence.
ACCESSION   DACDXO010000188 DACDXO010000000
VERSION     DACDXO010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639989
            Sequence Read Archive: SRR10023389
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741914.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:42:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,972
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 33 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 17 of 73
            Pseudo Genes (multiple problems)  :: 10 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..627
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639989-rid9881463.denovo.203"
                     /isolate="E23605_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..578
                     /locus_tag="IWM35_002862"
     CDS             <1..578
                     /locus_tag="IWM35_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="recombinase"
                     /protein_id="HAP9920624.1"
                     /translation="EREKALVASERVKIEEEKKNMLNVKKEMAVIFQKDIVPLESFKR
                     LPDGSYHLSGNDFVDLYQRATRATNAKRNADNMSEQYLQMLEQNTELNEYVATLEEER
                     HLSGRKQLADLQVENKKLKEENRSLRQRLERLKNAFQKSITRLSIRFGLAEKDMGLSE
                     ELEVLEQEKTLFYRQNVSQRKLERSEDEIEW"
BASE COUNT          216 a           87 c          157 g          167 t
ORIGIN      
        1 atgagcgaga aaaggctctg gtggcttctg agagagtgaa gattgaagaa gagaagaaaa
       61 atatgctgaa tgtaaaaaaa gagatggcgg tcattttcca aaaagatatt gttcctttag
      121 aaagtttcaa gaggcttcct gatggttcgt atcatttatc tgggaatgat tttgttgatt
      181 tgtatcaacg agctacgaga gcaacaaatg ccaagagaaa tgctgataac atgagtgagc
      241 aatatttaca gatgcttgag caaaatacag aattaaatga gtatgtagct acattggaag
      301 aagagcgtca tttgtctggt agaaaacaat tagcagattt acaagtggaa aataaaaagc
      361 tgaaagaaga aaatcgctct ttacgccagc gtttagagcg attaaaaaac gcatttcaaa
      421 aatcgatcac tcgtttgtct atccgttttg gtcttgcaga aaaggatatg gggctttctg
      481 aggaattaga agtgttagag caggagaaaa ccttatttta tcgtcaaaac gtctcacagc
      541 gtaaattaga gcgttctgaa gatgagatcg agtggtagtt acgatgtgca gtcaacggta
      601 ccggaggcgg tttgtgccga ggataag
//
LOCUS       DACDXO010000189          621 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E23605_2017
            SAMN12639989-rid9881463.denovo.204, whole genome shotgun sequence.
ACCESSION   DACDXO010000189 DACDXO010000000
VERSION     DACDXO010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639989
            Sequence Read Archive: SRR10023389
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 621)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 621)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741914.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:42:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,972
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 33 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 17 of 73
            Pseudo Genes (multiple problems)  :: 10 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..621
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639989-rid9881463.denovo.204"
                     /isolate="E23605_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..>621
                     /locus_tag="IWM35_002863"
     CDS             <1..>621
                     /locus_tag="IWM35_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS30-like element IS6770 family transposase"
                     /protein_id="HAP9920625.1"
                     /translation="LYRLFSKGIFDIDTLPMKGKRKPNGHQEKRGKQQYQRSIHDRPD
                     NYPDFNSEFGHLEGDTIVGIHHKSAVITLVERLSKVIITIKPNGRKALDIETALNQWF
                     SRFPKNFFKSITFDCGKEFSNWKAISNQHDIDIYFADPGTPSQRPLNENSNGILRRNG
                     LPKSMDFRKVNQTFISSVSNQRNHIPRKSLNYRTPIEIFLSYVQEA"
BASE COUNT          213 a          120 c          105 g          183 t
ORIGIN      
        1 cactttatcg tttattttct aaaggaatat ttgatattga cacactaccg atgaaaggta
       61 aaagaaaacc caatggccat caggaaaaac ggggaaaaca acagtatcag cgctcaatcc
      121 atgatcgacc tgataattat cctgatttca attctgagtt tggtcacctt gaaggtgata
      181 cgatcgttgg cattcatcat aaaagtgccg tcattacttt agttgaaaga ttatctaaag
      241 tcattatcac gattaaaccc aacggccgta aggcattaga tattgaaact gcccttaatc
      301 aatggttttc tcgcttccct aaaaacttct ttaaatctat tacgtttgac tgtggaaaag
      361 aattttctaa ctggaaagcc attagtaacc aacatgatat tgatatatat tttgcggacc
      421 ctggaacacc ttctcaacgc ccattaaacg agaattctaa cgggattctg cgtcgtaatg
      481 gactgccgaa atcaatggat tttagaaaag tgaatcagac atttatttcc agtgtcagca
      541 atcaacgtaa tcatattcca agaaaatcat tgaattacag aacaccaatc gaaatatttt
      601 tgagctatgt acaagaagca t
//
LOCUS       DACDXO010000190          598 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E23605_2017
            SAMN12639989-rid9881463.denovo.205, whole genome shotgun sequence.
ACCESSION   DACDXO010000190 DACDXO010000000
VERSION     DACDXO010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639989
            Sequence Read Archive: SRR10023389
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741914.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:42:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,972
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 33 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 17 of 73
            Pseudo Genes (multiple problems)  :: 10 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..598
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639989-rid9881463.denovo.205"
                     /isolate="E23605_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..491
                     /locus_tag="IWM35_002864"
     CDS             <1..491
                     /locus_tag="IWM35_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3-like element IS1485 family transposase"
                     /protein_id="HAP9920626.1"
                     /translation="VGKKWNYVCFILDLFNREILGYSCGEHKDAVLVKKAFSRIKQPL
                     TEVEIFHTDRGKEFDNQAIDELLTTFDINRSLSHKGCPFDNAVAESTYKSLKVEFVYQ
                     YTFETLQQLDLELFDYVNWWNHLRLHGTLGYETPVGYRNQRLAQRILDNELGCANASE
                     AV"
BASE COUNT          182 a          110 c          130 g          176 t
ORIGIN      
        1 gagtcgggaa aaaatggaat tatgtctgtt tcattttgga tctgttcaat cgagaaattc
       61 tcggctattc ttgtggagaa cataaagatg ccgttctagt aaaaaaagca tttagccgta
      121 tcaaacaacc tctgacagag gttgagattt ttcatactga tcgtggaaaa gagtttgata
      181 accaagctat tgatgaatta ttaacaactt ttgacatcaa tcgatcattg agtcataaag
      241 gctgtccttt tgataatgcc gtagctgaat caacttataa gtcgttgaaa gtagaatttg
      301 tctatcaata cacatttgaa accttacaac aattggattt ggagttattt gactatgtca
      361 attggtggaa ccaccttcgg ttgcacggta cacttggcta cgagacaccg gttggttacc
      421 gtaaccagag attggcgcag cgaatccttg ataatgagct cggatgtgct aacgctagcg
      481 aggcagtcta actttaactg ttagctcctg ccgaagatcg tcacatccga ggaggctcat
      541 tgtcaaggac aatcggaata gcatacggaa agaagttctg caccttataa aatttgtc
//
LOCUS       DACDXO010000191          595 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E23605_2017
            SAMN12639989-rid9881463.denovo.206, whole genome shotgun sequence.
ACCESSION   DACDXO010000191 DACDXO010000000
VERSION     DACDXO010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639989
            Sequence Read Archive: SRR10023389
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 595)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741914.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:42:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,972
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 33 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 17 of 73
            Pseudo Genes (multiple problems)  :: 10 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..595
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639989-rid9881463.denovo.206"
                     /isolate="E23605_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(1..563)
                     /locus_tag="IWM35_002865"
                     /pseudo
     CDS             complement(1..563)
                     /locus_tag="IWM35_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
BASE COUNT          168 a          109 c           78 g          240 t
ORIGIN      
        1 taattgtgca attgaaagta atagattcaa tttaaattct tttttgagct gaaggattgt
       61 tttaaccttg atttcttctt gctcaaatct tgttcttgaa tcaaggtttc taactttttt
      121 aaatatgcgt tctctgctct taaacgaata acttcttctt caagagactc atctttaagt
      181 tttttaggaa tgtttagctt ggaattcata ctaatttttc tgcccctttt ttggctctca
      241 agtgaagaag caccgccttc ttcatattgc tctatccatt tacttagagt tctatttgaa
      301 ccgatattaa attttttagc agtttcttgg atagaaagac catttgtttc catatattct
      361 ataacatcaa gtttaaattt tgtagtgtag cttttgccac ctccaaccaa gccttcccaa
      421 ccatgatagt tataaatcct tacccaatgt ctaaccagtg tacgatttat ttgatattta
      481 tgtgcaagat atttgtagcc gccttcgtta tttaaatagt ctgaaactac ttttttctta
      541 aaaacaaatg tatatttccg caaaaaaagc acccctttta attagatttc tagtc
//
LOCUS       DACDXO010000192          581 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E23605_2017
            SAMN12639989-rid9881463.denovo.207, whole genome shotgun sequence.
ACCESSION   DACDXO010000192 DACDXO010000000
VERSION     DACDXO010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639989
            Sequence Read Archive: SRR10023389
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741914.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:42:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,972
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 33 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 17 of 73
            Pseudo Genes (multiple problems)  :: 10 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..581
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639989-rid9881463.denovo.207"
                     /isolate="E23605_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..>581
                     /locus_tag="IWM35_002866"
     CDS             <1..>581
                     /locus_tag="IWM35_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK signal domain/LPXTG anchor domain surface
                     protein"
                     /protein_id="HAP9920627.1"
                     /translation="PITADDTEFVSSATKLDDIIENPGDNIPAGYHKVTFTAGEGTSI
                     ESGTTVFAVKDGVSLPEDKLPVLKAKDGYTDAKWPEEATQPITADDTEFVSSATKLDD
                     KSDADKYNPEGQKVTTELNKEPDAFEGIKNKEDLPKDTKYTWKEKVDVSAAGNKKGTV
                     VVTYPDGSSDEVEVDVTVTDNRSDADKYEPTVEG"
BASE COUNT          233 a           90 c          131 g          127 t
ORIGIN      
        1 ccaattacag cagatgatac agaatttgta tcaagtgcaa caaaattgga tgatatcatt
       61 gaaaacccag gggataacat tccggctggc tatcataaag taacctttac tgcaggagaa
      121 ggaacaagta ttgaaagtgg aacaacagtc tttgcagtga aagacggcgt aagcttacca
      181 gaagataaac ttccagtgtt gaaagcaaaa gatggttata cagatgcgaa atggccagaa
      241 gaagcaactc aaccaattac agcagatgat acagaatttg tatcaagtgc aacaaaactg
      301 gatgataaat ctgacgctga caaatataat cctgaaggtc aaaaagtgac tacagaattg
      361 aataaggaac cagacgcatt tgagggaatt aagaacaaag aagatttacc aaaagatact
      421 aagtatactt ggaaagaaaa agtagatgtt agcgcagctg gaaataaaaa agggactgtt
      481 gtagtgacat atccagatgg atcatctgat gaagttgaag tagatgttac agtgacagac
      541 aatcgttctg acgccgataa atatgagcca acagtagaag g
//
LOCUS       DACDXO010000193          526 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E23605_2017
            SAMN12639989-rid9881463.denovo.208, whole genome shotgun sequence.
ACCESSION   DACDXO010000193 DACDXO010000000
VERSION     DACDXO010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639989
            Sequence Read Archive: SRR10023389
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741914.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:42:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,972
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 33 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 17 of 73
            Pseudo Genes (multiple problems)  :: 10 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..526
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639989-rid9881463.denovo.208"
                     /isolate="E23605_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(6..359)
                     /gene="tnpB"
                     /locus_tag="IWM35_002867"
     CDS             complement(6..359)
                     /gene="tnpB"
                     /locus_tag="IWM35_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HAP9920628.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
BASE COUNT          153 a          116 c           90 g          167 t
ORIGIN      
        1 ccagcttaaa aaactccttt ttttcctggc aaaatagctc gaggttgatc gatacttaat
       61 ccttctaaca accatcgaac ctgttggttc gttaaggttc gaacttcgtc tttcgtccga
      121 ggccatttta acttgccgtt ttctatccgc ttataacaga gaagaaaacc atcaccgtcc
      181 caatagagaa gtttgaaacg gtcttgacgg ttcccgcaaa ataaaaaaac ggcatcttcg
      241 taaagctcga gttgatactc ttgttgaata agactggcaa gaccatcaat tccttttcgc
      301 aaatctgttt ttccgcacac aatataaata tgtttggttt gaagtaaatc ataaagcatt
      361 ggacaacagt tctttcaaaa tagcttgaag aatataattg ttaataccgt tatagatcaa
      421 aacttcagaa ccattctctc ttttgatgcg catcgctaac ttggcagccg cgggtttacc
      481 tttttctggt gcactctttt cgtttcctaa ttggacagga acaatc
//
LOCUS       DACDXO010000194          521 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E23605_2017
            SAMN12639989-rid9881463.denovo.209, whole genome shotgun sequence.
ACCESSION   DACDXO010000194 DACDXO010000000
VERSION     DACDXO010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639989
            Sequence Read Archive: SRR10023389
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741914.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:42:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,972
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 33 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 17 of 73
            Pseudo Genes (multiple problems)  :: 10 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..521
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639989-rid9881463.denovo.209"
                     /isolate="E23605_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            25..>521
                     /locus_tag="IWM35_002868"
     CDS             25..>521
                     /locus_tag="IWM35_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEf1 family transposase"
                     /protein_id="HAP9920629.1"
                     /translation="MNDFTTEIVQTLVTKGDLNELFRSHLEKAINTLLRTELTAFLDY
                     EKYDRTGFNSGNSRNGSYFRSIKTEYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLET
                     TIIHLFEKGVTMSEIADLIEKMYGHHYTPQTMSNMTKVLTEEVNAFKSRALNDKYVAI
                     FMDAT"
BASE COUNT          190 a           95 c           86 g          150 t
ORIGIN      
        1 attcacagaa aagaggactt ccctatgaat gattttacta cagaaattgt gcaaactcta
       61 gtcactaaag gcgatttaaa tgaattattc cgttcgcact tagaaaaagc gataaacaca
      121 ctcctacgga ctgaattaac ggctttttta gattacgaaa aatatgatcg cactggtttt
      181 aattcaggta attcgagaaa cggttcttac tttcgatcaa tcaaaaccga atatggtgaa
      241 ttaacattgg aaatacctag agatcgtaat ggtgagttta aacaacaaac tttaccagcc
      301 tacaaaagaa caaacgatac attggaaacc actattatcc atttattcga aaaaggtgtt
      361 acgatgtctg aaattgctga tttgatcgaa aaaatgtacg gtcatcacta tactccacaa
      421 accatgtcca acatgactaa agttctgact gaagaagtaa atgcctttaa atccagagcc
      481 ttaaatgata agtatgtcgc tatttttatg gacgctactt a
//
LOCUS       DACDXO010000195          514 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E23605_2017
            SAMN12639989-rid9881463.denovo.210, whole genome shotgun sequence.
ACCESSION   DACDXO010000195 DACDXO010000000
VERSION     DACDXO010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639989
            Sequence Read Archive: SRR10023389
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741914.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:42:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,972
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 33 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 17 of 73
            Pseudo Genes (multiple problems)  :: 10 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..514
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639989-rid9881463.denovo.210"
                     /isolate="E23605_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(107..>514)
                     /locus_tag="IWM35_002869"
     CDS             complement(107..>514)
                     /locus_tag="IWM35_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_465591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HAP9920630.1"
                     /translation="GTPENHFSNQISLNVYSRGMGGLGLPGDLSSVSRFVKATFTKMN
                     AASGDSESESISQFFHILGSVEQQKGVCDTGEGKYEYTIYSSCCNVDKGIYYYRTYED
                     SQITAVNMNKEDLDRHELVSYPVIKEQQINYVN"
BASE COUNT          162 a          103 c           78 g          171 t
ORIGIN      
        1 atcatccata aaatggactt tgtctacagt ctgagacacg acctaaagat cgtgtctaat
       61 tatgactgta tcagaaaata tgataccaaa cggatctatt cattaattaa ttgacatagt
      121 ttatctgttg ctcttttatg actggataac tgaccaattc atgacgatcc aagtcttctt
      181 tattcatatt tacagcagta atttgactgt cttcatacgt acgataataa taaatccctt
      241 tatcaacgtt acagcaagaa gaataaatcg tatattcgta ttttccttct ccagtatcgc
      301 atactccttt ttgttgctcg actgagccta gtatatgaaa aaattgactg atactttcag
      361 attctgaatc acctgatgca gcattcattt tagtaaatgt ggcttttaca aatcgagaaa
      421 cagaagagag atctccaggt aagcccaatc ctcccatccc acgactataa acattcaagc
      481 tgatctggtt tgaaaaatga ttttctggtg ttcc
//
LOCUS       DACDXO010000196          508 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E23605_2017
            SAMN12639989-rid9881463.denovo.211, whole genome shotgun sequence.
ACCESSION   DACDXO010000196 DACDXO010000000
VERSION     DACDXO010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639989
            Sequence Read Archive: SRR10023389
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741914.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:42:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,972
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 33 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 17 of 73
            Pseudo Genes (multiple problems)  :: 10 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..508
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639989-rid9881463.denovo.211"
                     /isolate="E23605_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
BASE COUNT          228 a           70 c           81 g          129 t
ORIGIN      
        1 aattccttag gcagcttttg ctgtgtagaa aggaataaat aataacgaaa gttcaaaagc
       61 taatgccgac tattctagac attaaagaag acgaaaacga acgataaaga aagaaaagtt
      121 atgaataatc gtttattgaa aaaaacttga aaaaatacaa aaaattcgtt gaccaagcta
      181 aatttagttg gtatattatt acttgtccaa aagacagaag aaaatattta ttcaaaaaca
      241 taacaaaaaa gttattgaca tgttactaac gacatgataa gataactgag tcgttaaaaa
      301 agaaccactt gcgaagcttg attctatcga aaaaaacttc aaaaaagttc ttgacaaata
      361 acaaacgata agttatgata aatgagttgc cgaaagattt cgaaaaacga aatcgaaaaa
      421 aacttttaaa aaagagttga caatcgcttg tctctctgat atgatataaa agttgctacg
      481 gcagccaaaa atgaatcggg aaacactc
//
LOCUS       DACDXO010000197          484 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E23605_2017
            SAMN12639989-rid9881463.denovo.212, whole genome shotgun sequence.
ACCESSION   DACDXO010000197 DACDXO010000000
VERSION     DACDXO010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639989
            Sequence Read Archive: SRR10023389
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741914.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:42:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,972
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 33 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 17 of 73
            Pseudo Genes (multiple problems)  :: 10 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..484
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639989-rid9881463.denovo.212"
                     /isolate="E23605_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(61..258)
                     /locus_tag="IWM35_002870"
     CDS             complement(61..258)
                     /locus_tag="IWM35_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP9920631.1"
                     /translation="MNNGTVKWFNSDKGFGFITGEDGNDVFAHFSAIQGEGFKSLDEG
                     QAVTYDIEEGQRGPQAVNIVK"
BASE COUNT          158 a           89 c           67 g          170 t
ORIGIN      
        1 aattttttct accgacataa acaaaaaaac cacctcatat gaggcggtta acgttcaaca
       61 ttattttacg atatttactg cttgaggacc acgttgtcct tcttcaatat cataagtaac
      121 tgcttggcct tcatctaaag acttaaagcc ttctccttgg attgctgaga aatgagcgaa
      181 tacgtcattt ccatcttcac cagtgatgaa accaaaacct ttatctgagt taaaccattt
      241 tactgtaccg ttattcatat atatttcctc ctgatacgta tataatacgt gttttattgc
      301 aattaatatt acttggtaaa aggagttttt atagtgtgaa tatatcgctc agattacgtt
      361 tcaaattaga ttacttattt actataacac ggatgtaatg ctaacacaag gataatcact
      421 cgatatacgg tttaaatatt attatttgtc aaattaagcg agacaaaact ttccatttac
      481 gtaa
//
LOCUS       DACDXO010000198          484 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E23605_2017
            SAMN12639989-rid9881463.denovo.213, whole genome shotgun sequence.
ACCESSION   DACDXO010000198 DACDXO010000000
VERSION     DACDXO010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639989
            Sequence Read Archive: SRR10023389
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741914.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:42:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,972
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 33 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 17 of 73
            Pseudo Genes (multiple problems)  :: 10 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..484
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639989-rid9881463.denovo.213"
                     /isolate="E23605_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..>484)
                     /locus_tag="IWM35_002871"
     CDS             complement(<1..>484)
                     /locus_tag="IWM35_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019117172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAP9920632.1"
                     /translation="SLLYNSGAITELGSVDKGTTRTDNTLLERQRGITIQTGITSFQW
                     ENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAKDGVQAQTRILFHALRKMGIPT
                     IFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVELYPNMCVTNFTESEQWDTVIEG
                     N"
BASE COUNT          131 a          105 c           75 g          173 t
ORIGIN      
        1 cgtttccctc tattaccgta tcccattgtt cagattcggt aaagttcgtc acacacatat
       61 taggatacag ttctaccttc tgtttgatta caatttcggc agaaagtttc tctttaatat
      121 cctgataaac cgttgataaa tcaattccat tttggtcaat cttattgata aaaaagattg
      181 tgggaatccc cattttccta agtgcatgaa ataatatacg agtttgtgct tgtacgccat
      241 cttttgcaga aatcagtaga attgccccat ctaaaactga taatgaacga tatacttctg
      301 ctaagaaatc catatgtcct ggcgtgtcta tgatgttcac cttcgtattt tcccactgaa
      361 aagaggttat tcctgtctga attgtaattc ctctctgacg ttctaaaagc gtattatccg
      421 tcctcgttgt acctttgtcc acgcttccta attctgtaat cgctccactg ttatataata
      481 agct
//
LOCUS       DACDXO010000199          465 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E23605_2017
            SAMN12639989-rid9881463.denovo.214, whole genome shotgun sequence.
ACCESSION   DACDXO010000199 DACDXO010000000
VERSION     DACDXO010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639989
            Sequence Read Archive: SRR10023389
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741914.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:42:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,972
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 33 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 17 of 73
            Pseudo Genes (multiple problems)  :: 10 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..465
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639989-rid9881463.denovo.214"
                     /isolate="E23605_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            10..375
                     /locus_tag="IWM35_002872"
     CDS             10..375
                     /locus_tag="IWM35_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9920633.1"
                     /translation="MVFDIDNLKGFLNDTSSFGFIAKENNKIIGFAYCYTLLRPDGKT
                     MFYLHSIGMLPNYQDKGYGSKLLSFIKEYSKEIGCSEMFLITDKGNPRACHVYEKLGG
                     KNDYKDEIVYVYDYEKGDK"
     gene            376..>465
                     /locus_tag="IWM35_002873"
     CDS             376..>465
                     /locus_tag="IWM35_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase"
                     /protein_id="HAP9920634.1"
                     /translation="MNIVENEICIRTLIDDDFPLMLKWLTDERV"
BASE COUNT          184 a           34 c           80 g          167 t
ORIGIN      
        1 gatgataata tggtttttga tattgataat ttaaaaggat ttcttaatga taccagttca
       61 tttgggttta tagctaaaga aaataataaa attataggat ttgcatattg ctatacactt
      121 ttaagacctg atggaaaaac aatgttttat ttacactcaa taggaatgtt acctaactat
      181 caagacaaag gttatggttc aaaattatta tcttttatta aggaatattc taaagagatt
      241 ggttgttctg aaatgttttt aataactgat aaaggtaatc ctagagcttg ccatgtatat
      301 gaaaaattag gtggtaaaaa tgattataaa gatgaaatag tatatgtata tgattatgaa
      361 aaaggtgata aataaatgaa tatagttgaa aatgaaatat gtataagaac tttaatagat
      421 gatgattttc ctttgatgtt aaaatggtta actgatgaaa gagta
//
LOCUS       DACDXO010000200          441 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E23605_2017
            SAMN12639989-rid9881463.denovo.215, whole genome shotgun sequence.
ACCESSION   DACDXO010000200 DACDXO010000000
VERSION     DACDXO010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639989
            Sequence Read Archive: SRR10023389
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741914.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:42:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,972
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 33 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 17 of 73
            Pseudo Genes (multiple problems)  :: 10 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..441
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639989-rid9881463.denovo.215"
                     /isolate="E23605_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..>441
                     /locus_tag="IWM35_002874"
     CDS             <1..>441
                     /locus_tag="IWM35_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAP9920635.1"
                     /translation="DKYSKIKQEITAIVKESRNSYGYRRVTLALKMKGYTINHKTVRK
                     LMSQMGLTCQIRIKRYKSYKGTVGKIAKNVLKRNFSVDTPNKKWVTDVTEFKIKGRKI
                     YLSPILDLFNGEIISYSISTSPTYKLIEEMLQQAIKKKGTEGSL"
BASE COUNT          194 a           61 c           74 g          112 t
ORIGIN      
        1 cagataaata tagcaagata aagcaagaaa ttacagcgat tgtaaaagaa tcaagaaact
       61 cttatggtta tcgtagagtc actttagcgt taaagatgaa aggatataca atcaatcata
      121 aaacagttag aaaattaatg tcccaaatgg gacttacctg tcaaatccga ataaagcgat
      181 ataaatctta taaaggaaca gtaggaaaaa ttgccaagaa tgtgttaaaa cgaaattttt
      241 cagtagatac acccaataaa aaatgggtga cagacgtcac tgaattcaag ataaaaggaa
      301 gaaaaatcta tctatctcct atacttgatt tatttaacgg agaaataatt agttatagta
      361 tatcaactag tccaacatat aaactgattg aagaaatgct tcaacaagca attaaaaaaa
      421 agggaactga gggttcactg a
//
LOCUS       DACDXO010000201          429 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E23605_2017
            SAMN12639989-rid9881463.denovo.216, whole genome shotgun sequence.
ACCESSION   DACDXO010000201 DACDXO010000000
VERSION     DACDXO010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639989
            Sequence Read Archive: SRR10023389
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741914.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:42:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,972
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 33 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 17 of 73
            Pseudo Genes (multiple problems)  :: 10 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..429
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639989-rid9881463.denovo.216"
                     /isolate="E23605_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..>429
                     /locus_tag="IWM35_002875"
     CDS             <1..>429
                     /locus_tag="IWM35_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP9920636.1"
                     /translation="KIPHFSTFGKNYVRRFRETTVFEDIFAYILEQAVKAGFVTEDNL
                     YLDSTHIKANANKHKFTKEMTHDEAKAYQDELEDEINRQRIEAGKRPFTLDLEKEVKL
                     KERKISKADPESGYYVKGEREKQFAYSAHTSCDDNGFILST"
BASE COUNT          157 a           75 c           88 g          109 t
ORIGIN      
        1 aaaatccccc atttctcaac ttttggaaag aattatgttc gccgatttag agaaacgacg
       61 gtgtttgagg atatttttgc ctatatttta gaacaagcag ttaaagcagg ttttgtaaca
      121 gaggataatt tgtatctgga ttctacccac atcaaagcta acgccaataa acataagttt
      181 acgaaagaaa tgacccatga tgaagcgaaa gcctatcagg atgaattaga agatgagatc
      241 aatcgtcaac gaatagaggc tggaaaacgc ccttttactt tggacttaga aaaggaagtg
      301 aagctgaaag aaagaaaaat aagtaaagct gatccggaaa gcggctacta cgtcaaaggc
      361 gaacgagaaa aacaatttgc ttattctgcc catacttcat gcgacgacaa tggatttatt
      421 cttagcaca
//
LOCUS       DACDXO010000202          415 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E23605_2017
            SAMN12639989-rid9881463.denovo.218, whole genome shotgun sequence.
ACCESSION   DACDXO010000202 DACDXO010000000
VERSION     DACDXO010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639989
            Sequence Read Archive: SRR10023389
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741914.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:42:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,972
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 33 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 17 of 73
            Pseudo Genes (multiple problems)  :: 10 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..415
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639989-rid9881463.denovo.218"
                     /isolate="E23605_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..390
                     /locus_tag="IWM35_002876"
     CDS             <1..390
                     /locus_tag="IWM35_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAP9920637.1"
                     /translation="KDRKEICDDFKAVYQANSKEEANTFLSGMIEKWKKNYPKVTQSL
                     IENQDLLTFYDFPPSIRRTIYSTNLIESFNKQIKRYSRRKEQFQNEESLERFLVSIFD
                     TYNQKFLNRSHKGFQQVTDTLVSMFTE"
BASE COUNT          161 a           74 c           65 g          115 t
ORIGIN      
        1 aaagaccgaa aagaaatctg tgatgacttt aaggctgttt atcaagctaa ctcaaaagaa
       61 gaagcgaata ccttcttatc cggcatgatt gagaaatgga agaaaaacta tcctaaagtg
      121 acgcagtcac tcatagaaaa ccaagactta ttaacttttt atgattttcc acctagcatt
      181 cgtagaacca tttactcaac caatctaatc gagtctttca ataagcaaat taaaagatac
      241 agccgtagaa aagagcagtt tcaaaatgaa gaatcactag aacgctttct agtcagcatt
      301 tttgatacat acaatcaaaa atttctaaac agaagccata aaggttttca acaggtaacc
      361 gatacattag tttcaatgtt tactgagtaa ctaattattt tgcaggagga caatt
//
LOCUS       DACDXO010000203          415 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E23605_2017
            SAMN12639989-rid9881463.denovo.219, whole genome shotgun sequence.
ACCESSION   DACDXO010000203 DACDXO010000000
VERSION     DACDXO010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639989
            Sequence Read Archive: SRR10023389
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741914.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:42:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,972
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 33 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 17 of 73
            Pseudo Genes (multiple problems)  :: 10 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..415
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639989-rid9881463.denovo.219"
                     /isolate="E23605_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..339)
                     /locus_tag="IWM35_002877"
     CDS             complement(<1..339)
                     /locus_tag="IWM35_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9920638.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYT"
BASE COUNT          116 a           70 c           68 g          161 t
ORIGIN      
        1 tgtataactc tgaacaggat ttttcttact tttgaaacgt ggaaatccaa cggatttatc
       61 ccgaaaaaaa ttcttgtatg ctttatctaa attaagttgg gcattggcta aagcaaggct
      121 atcaatttct ttcaaaaacg gaaattcttt cttgtattta gctggtgtcg gaaaagtcat
      181 ttttttagaa gaatcatttt taacttcttc atacgccttt tttcggtcat caagcattag
      241 attgtagacc ttacggacac aaccaaaaga tttggcaaag aagatttctt gttcctctgt
      301 tggatagatt ctgaatttgt atgcttttag ttgttccata aagttcacct actttctatt
      361 tatgattgtt tccttgattt tggatatact tcttaatcac atcaattggt gcgcc
//
LOCUS       DACDXO010000204          406 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E23605_2017
            SAMN12639989-rid9881463.denovo.220, whole genome shotgun sequence.
ACCESSION   DACDXO010000204 DACDXO010000000
VERSION     DACDXO010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639989
            Sequence Read Archive: SRR10023389
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741914.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:42:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,972
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 33 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 17 of 73
            Pseudo Genes (multiple problems)  :: 10 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..406
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639989-rid9881463.denovo.220"
                     /isolate="E23605_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..299
                     /locus_tag="IWM35_002878"
     CDS             <1..299
                     /locus_tag="IWM35_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9920639.1"
                     /translation="DLREETVDRIELMYERLADYLNLPDEDNYSEMLERIMDVLNSVI
                     NADLAKSSVLTNEFVHKHLAQTTDESEKEFWLKLMSHVMQLNQISVINSKEKEM"
BASE COUNT          159 a           47 c           82 g          118 t
ORIGIN      
        1 ttgatttgag agaagaaaca gtcgatagaa ttgaattgat gtatgaacgg ttagctgatt
       61 atttgaattt gccggatgaa gataattata gtgaaatgct tgaacgaatt atggatgttc
      121 tcaactcggt tatcaatgct gatttagcaa aatcttcagt tttgactaat gaatttgttc
      181 ataagcattt agcgcaaaca actgatgaat cagaaaaaga attttggttg aaattaatga
      241 gtcatgttat gcagttaaac caaatttccg ttattaattc caaagaaaag gagatgtagt
      301 ttatgggaat gactttagca gatattaaag cgcaagaaga aaaattaaaa aaagaacaag
      361 cgaaacttcg ccaacagaaa aaacgcttga ttgagaaaga aaatgc
//
LOCUS       DACDXO010000205          388 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E23605_2017
            SAMN12639989-rid9881463.denovo.221, whole genome shotgun sequence.
ACCESSION   DACDXO010000205 DACDXO010000000
VERSION     DACDXO010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639989
            Sequence Read Archive: SRR10023389
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741914.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:42:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,972
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 33 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 17 of 73
            Pseudo Genes (multiple problems)  :: 10 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..388
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639989-rid9881463.denovo.221"
                     /isolate="E23605_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            22..>388
                     /locus_tag="IWM35_002879"
     CDS             22..>388
                     /locus_tag="IWM35_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP9920640.1"
                     /translation="MTYTHLTPNELVMIEAYFHQETPVAIVAKQLKRGRQTIYNVYNF
                     LKCGGTALEYFEQYKENKRRCGRTEIIFPAEEKEYIEKRSTEGWTPDVIIGRAERTFS
                     CSVSTLYRRFKTGEFNVLHL"
BASE COUNT          133 a           76 c           77 g          102 t
ORIGIN      
        1 atacaaggag agtgaacata aatgacttat acccatctta caccaaacga gcttgtaatg
       61 atagaagcat attttcatca agaaactccg gttgctatcg ttgcgaagca gcttaaacgt
      121 ggacgccaaa caatttacaa tgtctataac tttctcaaat gtggtggaac agcacttgaa
      181 tactttgaac aatacaaaga aaataagcga cgttgtggga gaaccgaaat catttttcct
      241 gctgaggaaa aagaatacat tgaaaaaaga tcaactgaag gttggacccc agacgtcatt
      301 attggccgtg cagagcgaac cttctcttgt tcagtaagta ccctctatcg ccggtttaag
      361 acgggagaat tcaatgtttt acatttac
//
LOCUS       DACDXO010000206          358 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E23605_2017
            SAMN12639989-rid9881463.denovo.222, whole genome shotgun sequence.
ACCESSION   DACDXO010000206 DACDXO010000000
VERSION     DACDXO010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639989
            Sequence Read Archive: SRR10023389
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741914.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:42:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,972
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 33 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 17 of 73
            Pseudo Genes (multiple problems)  :: 10 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..358
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639989-rid9881463.denovo.222"
                     /isolate="E23605_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..>358)
                     /locus_tag="IWM35_002880"
     CDS             complement(<1..>358)
                     /locus_tag="IWM35_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
                     /protein_id="HAP9920641.1"
                     /translation="RNPNLSVYNAVIHNDEVSPHLHLNIVPVAEGYKRGVQKQPSFNK
                     ALQQQGLRADKENGRVIWNNFRNQEVESIERLMADFGWERELVGTNKIKDVHEYKEIM
                     SEISELRQTAVKERESV"
BASE COUNT           98 a           90 c           54 g          116 t
ORIGIN      
        1 aaacgctctc acgctctttt acggccgttt gacgcaattc tgaaatctcg ctcatgattt
       61 ctttatattc gtgaacatct ttgattttgt tagtaccgac taactcacgc tcccaaccaa
      121 aatctgccat caaacgttca attgactcaa cttcctgatt cctaaaatta ttccaaatca
      181 ctcgaccatt ctctttatct gctcgtaaac cctgctgttg caaggctttg ttaaaacttg
      241 gttgcttttg aacaccccgt ttataccctt cagcaacagg aacaatattc aaatgcaggt
      301 gcggacttac ttcgtcatta tgaataacag cattgtagac ggataaatta ggattccg
//
LOCUS       DACDXO010000207          345 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E23605_2017
            SAMN12639989-rid9881463.denovo.223, whole genome shotgun sequence.
ACCESSION   DACDXO010000207 DACDXO010000000
VERSION     DACDXO010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639989
            Sequence Read Archive: SRR10023389
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741914.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:42:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,972
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 33 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 17 of 73
            Pseudo Genes (multiple problems)  :: 10 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..345
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639989-rid9881463.denovo.223"
                     /isolate="E23605_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..>345
                     /locus_tag="IWM35_002881"
     CDS             <1..>345
                     /locus_tag="IWM35_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9920642.1"
                     /translation="GVSKNTVRNQLVNSGFDLAQYKENGIIMLDKELIGVVRSIYRSK
                     SDQLTGQLPVNNTGSDFPLEKENEFLRDQLAEKDKHISELTEMIRDQQKLTLLQQQKN
                     DQLLLENQELKNR"
BASE COUNT          127 a           52 c           70 g           96 t
ORIGIN      
        1 gggtgtatcg aaaaatacag tgcgcaatca actggtcaat tcagggtttg accttgctca
       61 gtataaagaa aatggcatta tcatgctaga taaagagttg attggtgttg ttcggtcaat
      121 ttatcggtca aaaagtgatc aattgactgg tcaattaccg gtcaataaca caggcagtga
      181 ttttccttta gaaaaagaga atgagttttt gcgtgatcaa ttagctgaga aggacaaaca
      241 tatttctgaa ttaacagaaa tgattcgtga tcaacaaaaa ttgaccctgc ttcagcaaca
      301 aaaaaacgat cagttgttac tagaaaatca agaattaaaa aatcg
//
LOCUS       DACDXO010000208          338 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E23605_2017
            SAMN12639989-rid9881463.denovo.224, whole genome shotgun sequence.
ACCESSION   DACDXO010000208 DACDXO010000000
VERSION     DACDXO010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639989
            Sequence Read Archive: SRR10023389
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741914.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:42:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,972
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 33 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 17 of 73
            Pseudo Genes (multiple problems)  :: 10 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..338
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639989-rid9881463.denovo.224"
                     /isolate="E23605_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            1..338
                     /locus_tag="IWM35_002882"
                     /pseudo
     CDS             1..338
                     /locus_tag="IWM35_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          142 a           59 c           64 g           73 t
ORIGIN      
        1 gtaaagcaat ctaaaacgag tggttcattc aaagaaaaag ataatcttac gcctgaacaa
       61 aaagaattgt tagaactacg taaaagaaac cagcaattag aaatggaaaa tgatatttta
      121 aagcaagcag cgctgatatt cggacgaaga gacaagtaat cgatgcgaat aagcatcttt
      181 accctatatc agcgatgtgc agaatattag gtctatcacg tcagtcctat tattatcaat
      241 caaaaccaaa gaaagacgaa tcagaacttg aagaagtagt cgctgaagaa tttatccgca
      301 gccgaaaggc ctacggctca agaaaaataa aaaaagcc
//
LOCUS       DACDXO010000209          331 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E23605_2017
            SAMN12639989-rid9881463.denovo.225, whole genome shotgun sequence.
ACCESSION   DACDXO010000209 DACDXO010000000
VERSION     DACDXO010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639989
            Sequence Read Archive: SRR10023389
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741914.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:42:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,972
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 33 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 17 of 73
            Pseudo Genes (multiple problems)  :: 10 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..331
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639989-rid9881463.denovo.225"
                     /isolate="E23605_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..>331
                     /locus_tag="IWM35_002883"
     CDS             <1..>331
                     /locus_tag="IWM35_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS6-like element ISS1D family transposase"
                     /protein_id="HAP9920643.1"
                     /translation="YLWKKKNRQSFYSWKMDETYIKIKGRWHYLYRAIDADGLTLDIW
                     LRKKRDTQAAYAFLKRLHKQFGEPKAIVTDKAPSLGSAFRKLQSVGLYTKTEHRTVKY
                     LNNLIEQ"
BASE COUNT          114 a           60 c           68 g           89 t
ORIGIN      
        1 attatctttg gaagaagaaa aatagacaat ccttctattc atggaaaatg gacgaaacct
       61 atatcaaaat taagggacgt tggcattatc tttatcgtgc aattgatgcg gacggcttaa
      121 ccttagatat ctggttacga aagaaacggg atacgcaagc agcctatgct ttcttaaaac
      181 gactccataa acagtttggt gagccgaaag caattgtgac cgataaagca ccttctcttg
      241 gctccgcctt tagaaagtta cagagtgtgg gtttatatac taagacagag caccgaactg
      301 tgaagtatct taacaattta atagaacaag a
//
LOCUS       DACDXO010000210          329 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E23605_2017
            SAMN12639989-rid9881463.denovo.226, whole genome shotgun sequence.
ACCESSION   DACDXO010000210 DACDXO010000000
VERSION     DACDXO010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639989
            Sequence Read Archive: SRR10023389
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 329)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 329)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741914.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:42:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,972
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 33 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 17 of 73
            Pseudo Genes (multiple problems)  :: 10 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..329
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639989-rid9881463.denovo.226"
                     /isolate="E23605_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(13..246)
                     /locus_tag="IWM35_002884"
     CDS             complement(13..246)
                     /locus_tag="IWM35_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9920644.1"
                     /translation="MTEKQKYFGKKNWLVNLTGDYVVVVFLDSKKMSGKLLDVTQYEL
                     ILKNNKIDSPIIIPKHAVKYIYAVAESSGEAND"
BASE COUNT           94 a           73 c           39 g          123 t
ORIGIN      
        1 ttttctgaca aatcagtcat tagcttctcc actactttca gctaccgcat aaatatattt
       61 aacggcgtgc ttagggataa taatcggaga gtcaatttta ttatttttca aaatcaattc
      121 atattgcgtt acatcaagta acttaccgga catttttttg ctatccaaaa aaacaaccac
      181 aacgtaatca cctgttaaat ttaccaacca atttttctta ccaaaatact tttgtttttc
      241 tgtcattttt cttcctcctc tgttacgtct agccctagaa gccatgtgtc taattctttt
      301 tttaaatcgt ctgccgattc cgcaaaggt
//
LOCUS       DACDXO010000211          328 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E23605_2017
            SAMN12639989-rid9881463.denovo.227, whole genome shotgun sequence.
ACCESSION   DACDXO010000211 DACDXO010000000
VERSION     DACDXO010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639989
            Sequence Read Archive: SRR10023389
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741914.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:42:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,972
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 33 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 17 of 73
            Pseudo Genes (multiple problems)  :: 10 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..328
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639989-rid9881463.denovo.227"
                     /isolate="E23605_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            52..>328
                     /locus_tag="IWM35_002885"
     CDS             52..>328
                     /locus_tag="IWM35_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HAP9920645.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVSEYRTYLELKKQRFKCK"
BASE COUNT          104 a           60 c           63 g          101 t
ORIGIN      
        1 cctgaaatcc tttagaatta atttggctac caattcatag gaggattcaa gatgtctcat
       61 actcagctta tcaaagatac cctaaatatt ctagacctaa atatctattt cgaggaaaat
      121 tgtttaacta aagaaaaata caaagggcaa atttgcttga tttacaaagg aactcttctg
      181 tacaagccag aagagtgttc ccattgtctt tgtgtcgtcc cctctaggat cattcgttgg
      241 gggacgacaa ccgttcggtt gttactgaat gatgtgtctg agtatcgcac ttatctggag
      301 ctgaagaaac aacgatttaa atgtaagt
//
LOCUS       DACDXO010000212          323 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E23605_2017
            SAMN12639989-rid9881463.denovo.228, whole genome shotgun sequence.
ACCESSION   DACDXO010000212 DACDXO010000000
VERSION     DACDXO010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639989
            Sequence Read Archive: SRR10023389
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741914.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:42:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,972
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 33 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 17 of 73
            Pseudo Genes (multiple problems)  :: 10 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..323
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639989-rid9881463.denovo.228"
                     /isolate="E23605_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..310
                     /locus_tag="IWM35_002886"
     CDS             <1..310
                     /locus_tag="IWM35_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAP9920646.1"
                     /translation="QIRFMIDKWKKQYPRVVKLLMNPAILTFYNFPPSIRRTIYSTNL
                     IEGFNKQLKKYTKRKEQFPNEESLERFLVSQFNNYNQKFLCRVHKGFKEIHDTLESMF
                     "
BASE COUNT          129 a           48 c           45 g          101 t
ORIGIN      
        1 tcaaataagg tttatgatag ataaatggaa aaagcagtat ccacgagtgg ttaaattgct
       61 catgaatcct gctatattaa ccttttataa tttccctccg tcaatcagaa gaactattta
      121 ttcaactaat ttgattgaag gttttaacaa acaattaaag aaatatacca aaagaaaaga
      181 gcaatttcct aatgaagaat ctctggagag atttctagta tctcaattca acaactataa
      241 tcaaaagttt ctatgccgag ttcataaagg ttttaaagaa atacatgata cattagaatc
      301 aatgttttaa cttgatcaac aga
//
LOCUS       DACDXO010000213          321 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E23605_2017
            SAMN12639989-rid9881463.denovo.229, whole genome shotgun sequence.
ACCESSION   DACDXO010000213 DACDXO010000000
VERSION     DACDXO010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639989
            Sequence Read Archive: SRR10023389
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741914.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:42:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,972
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 33 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 17 of 73
            Pseudo Genes (multiple problems)  :: 10 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..321
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639989-rid9881463.denovo.229"
                     /isolate="E23605_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..>321)
                     /locus_tag="IWM35_002887"
     CDS             complement(<1..>321)
                     /locus_tag="IWM35_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP9920647.1"
                     /translation="ITRHVWGDLMDEVEHLRLTDLNKSIYKKRKQTIERIFADAKEKH
                     GMRWTKYRGLEKVATHTMLVFAAMNLKKLATWLWKGKEPLFFCSKIRNEVDKKLFQAR
                     VTSL"
BASE COUNT           79 a           78 c           51 g          113 t
ORIGIN      
        1 tccaaactcg taactcgagc ttggaacagc tttttatcta cttcgtttcg aatttttgaa
       61 caaaaaaata aaggctcctt gcctttccat agccaggtgg ctaatttctt taaattcatt
      121 gcagcaaaca ctagcatcgt atgcgtcgcg actttttcca agcctcgata cttggtccaa
      181 cgcatcccat gcttttcttt tgcatccgca aatattcgtt caatcgtttg tttccgtttt
      241 ttgtaaattg atttattcag atctgttagg cgaagatgtt ccacttcatc catcagatct
      301 ccccaaacat gtctcgtgat g
//
LOCUS       DACDXO010000214          317 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E23605_2017
            SAMN12639989-rid9881463.denovo.230, whole genome shotgun sequence.
ACCESSION   DACDXO010000214 DACDXO010000000
VERSION     DACDXO010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639989
            Sequence Read Archive: SRR10023389
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741914.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:42:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,972
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 33 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 17 of 73
            Pseudo Genes (multiple problems)  :: 10 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..317
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639989-rid9881463.denovo.230"
                     /isolate="E23605_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..>317
                     /locus_tag="IWM35_002888"
     CDS             <1..>317
                     /locus_tag="IWM35_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP9920648.1"
                     /translation="RQAKLLNQIAKVGYNSTKKSYFYGLKIHMIVTKTGFPITYSITN
                     PGVHDVKVLETLSEEANLPNILGDKGYISHKIHEKLALKGITISVPPRKNMDKSEKLD
                     HSL"
BASE COUNT          117 a           64 c           52 g           84 t
ORIGIN      
        1 tagacaagca aaactattga atcaaatagc caaagttggc tataactcaa caaaaaaatc
       61 ttatttttat ggtcttaaaa ttcatatgat cgttactaag actggctttc cgattactta
      121 ctcaatcacg aatccaggtg tccatgacgt gaaagtgtta gagactttat cggaagaagc
      181 gaatcttccc aatatcctag gggacaaagg atatattagc cataaaatcc atgaaaagct
      241 tgcccttaag ggcattacta tatccgttcc gccacgtaaa aacatggata aatcagaaaa
      301 actagaccac agcttac
//
LOCUS       DACDXO010000215          313 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E23605_2017
            SAMN12639989-rid9881463.denovo.231, whole genome shotgun sequence.
ACCESSION   DACDXO010000215 DACDXO010000000
VERSION     DACDXO010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639989
            Sequence Read Archive: SRR10023389
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741914.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:42:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,972
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 33 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 17 of 73
            Pseudo Genes (multiple problems)  :: 10 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..313
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639989-rid9881463.denovo.231"
                     /isolate="E23605_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..294
                     /locus_tag="IWM35_002889"
     CDS             <1..294
                     /locus_tag="IWM35_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP9920649.1"
                     /translation="SSTTSDSSDSSTSSDSTDTSSTTSDTRHASVSFSKTSSDNRTNF
                     NLTNKTSNDNGNKSKRALPKTGSQSNNWITLAGVILLVIGLRITFMSYKKSKR"
BASE COUNT          127 a           61 c           49 g           76 t
ORIGIN      
        1 tcctctacca caagtgatag ctcagattca tcaacttcaa gcgatagtac agacacgtcg
       61 tctaccacaa gcgatacacg tcatgcaagt gtctccttct caaaaacaag ttcggataac
      121 aggacgaact ttaacttaac taataaaaca agcaatgaca atggaaacaa atcaaaaaga
      181 gcacttccta aaactggaag tcaatcgaat aattggataa cactcgcagg cgttatatta
      241 cttgtaatcg gtttaagaat cacttttatg agttataaga aatcgaaaag gtaaaaaaat
      301 attcaagata aaa
//
LOCUS       DACDXO010000216          309 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E23605_2017
            SAMN12639989-rid9881463.denovo.232, whole genome shotgun sequence.
ACCESSION   DACDXO010000216 DACDXO010000000
VERSION     DACDXO010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639989
            Sequence Read Archive: SRR10023389
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741914.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:42:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,972
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 33 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 17 of 73
            Pseudo Genes (multiple problems)  :: 10 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..309
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639989-rid9881463.denovo.232"
                     /isolate="E23605_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            51..>309
                     /locus_tag="IWM35_002890"
     CDS             51..>309
                     /locus_tag="IWM35_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HAP9920650.1"
                     /translation="MNHFKGKQFQQDVIIVAVGYYLRYNLSYREVQEILYDRGINVSH
                     TTIYRWVQEYGKLLYQIWKKKNKKSFYSWKMDETYIKIKGKW"
BASE COUNT          120 a           41 c           61 g           87 t
ORIGIN      
        1 tactatcaaa taaggtagaa taatagaaaa agatagcagg aggaatgacg atgaatcatt
       61 ttaaaggaaa gcaatttcag caggatgtga ttattgtagc cgtgggctac tatcttcgtt
      121 ataaccttag ctatcgtgaa gttcaagaaa tcttatatga tcgtggcatt aacgtttctc
      181 atacgacgat ttatcgttgg gtgcaagaat atggcaaact actctatcaa atttggaaaa
      241 agaaaaataa aaaatccttt tattcatgga aaatggatga aacgtacatc aaaattaaag
      301 gaaaatggc
//
LOCUS       DACDXO010000217          308 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E23605_2017
            SAMN12639989-rid9881463.denovo.233, whole genome shotgun sequence.
ACCESSION   DACDXO010000217 DACDXO010000000
VERSION     DACDXO010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639989
            Sequence Read Archive: SRR10023389
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741914.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:42:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,972
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 33 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 17 of 73
            Pseudo Genes (multiple problems)  :: 10 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..308
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639989-rid9881463.denovo.233"
                     /isolate="E23605_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(76..>308)
                     /locus_tag="IWM35_002891"
     CDS             complement(76..>308)
                     /locus_tag="IWM35_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP9920651.1"
                     /translation="LKNLPETLDAQLRTKLQNLLTYEEGIYNAMIYPYSNGKIEAKIP
                     HIKTLKRLSYGFKSFENMKIRIFLINQLIQVK"
BASE COUNT           94 a           46 c           56 g          112 t
ORIGIN      
        1 atgttggtga agttcaccaa catcaaaaag tatagagccg atagaaggac tgaccttctg
       61 tctcagattt ttttgttatt tcacttgaat caattgattg atcaaaaaaa tcctgatttt
      121 catgttctca aatgatttaa atccataaga taatcgtttc agtgtcttga tatggggaat
      181 cttggcctct attttgccat tagaatacgg gtaaatcata gcattgtaaa taccttcttc
      241 atacgttaaa aggttttgaa gtttcgtgcg aagttgtgca tcaagtgttt caggaagatt
      301 ttttaata
//
LOCUS       DACDXO010000218          305 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E23605_2017
            SAMN12639989-rid9881463.denovo.234, whole genome shotgun sequence.
ACCESSION   DACDXO010000218 DACDXO010000000
VERSION     DACDXO010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639989
            Sequence Read Archive: SRR10023389
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741914.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:42:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,972
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 33 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 17 of 73
            Pseudo Genes (multiple problems)  :: 10 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..305
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639989-rid9881463.denovo.234"
                     /isolate="E23605_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..302)
                     /locus_tag="IWM35_002892"
     CDS             complement(<1..302)
                     /locus_tag="IWM35_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="HAP9920652.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERIS
                     ESYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLW"
BASE COUNT           95 a           45 c           50 g          115 t
ORIGIN      
        1 ttccaaagaa attgtttgac tcttggaaag tcacgtttta tcaatcttga gctggcactt
       61 ttataggcat tgatgaattt tgtcaattct gttttaggtt gagctttgaa cataatatga
      121 acgtggtctt tatcatgatt ccactccact aaagtaatgt gatacgattc tgaaattctt
      181 tcaaaagtag ttttggcata atcagatatt tcatcatcaa tcacttgtct acgatatttc
      241 gttactaaaa caaggtggta ataaagaaga aatactgaat gattatttgt atctaattcc
      301 atgtt
//
LOCUS       DACDXO010000219          298 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E23605_2017
            SAMN12639989-rid9881463.denovo.235, whole genome shotgun sequence.
ACCESSION   DACDXO010000219 DACDXO010000000
VERSION     DACDXO010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639989
            Sequence Read Archive: SRR10023389
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 298)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741914.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:42:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,972
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 33 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 17 of 73
            Pseudo Genes (multiple problems)  :: 10 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..298
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639989-rid9881463.denovo.235"
                     /isolate="E23605_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..>298
                     /locus_tag="IWM35_002893"
     CDS             <1..>298
                     /locus_tag="IWM35_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS6-like element IS1216 family transposase"
                     /protein_id="HAP9920653.1"
                     /translation="IWLRKKRDTQAAYAFLKRLVKQFDEPKVVVTDKAPSITSAFKKL
                     KEYGFYQGTEHRTIKYLNNLIEQDHRPVKRRNKFYRSLRTASTTIKGMEAIRGLY"
BASE COUNT          104 a           59 c           58 g           77 t
ORIGIN      
        1 tatttggtta cgtaaaaaac gggacacaca agcagcctat gcttttctta agcggttagt
       61 gaagcagttt gatgaaccga aggttgtagt cacagataaa gccccctcta ttacaagtgc
      121 ctttaagaaa ctaaaagaat acggctttta tcaagggaca gaacatcgta ccattaaata
      181 cctgaataat ttgattgaac aagaccatcg tccagtaaag agacgcaata aattctatcg
      241 aagtttacgc actgcctcta ccacgattaa aggcatggaa gccattcgag gattatat
//
LOCUS       DACDXO010000220          291 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E23605_2017
            SAMN12639989-rid9881463.denovo.236, whole genome shotgun sequence.
ACCESSION   DACDXO010000220 DACDXO010000000
VERSION     DACDXO010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639989
            Sequence Read Archive: SRR10023389
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741914.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:42:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,972
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 33 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 17 of 73
            Pseudo Genes (multiple problems)  :: 10 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..291
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639989-rid9881463.denovo.236"
                     /isolate="E23605_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..>291
                     /locus_tag="IWM35_002894"
     CDS             <1..>291
                     /locus_tag="IWM35_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HAP9920654.1"
                     /translation="LVKQSKRLFPEINCVVADAGYKTPKFVHFLTHLNLRPCLPYSRP
                     KGKKGLLSKNEFLYDEYFDCYICPQDQMLAFSTVTREGYREYKSNPKECVNC"
BASE COUNT           86 a           55 c           58 g           92 t
ORIGIN      
        1 agctcgtcaa acaatcgaag agactttttc ctgaaatcaa ttgtgttgtc gctgatgctg
       61 ggtataagac acctaagttt gttcactttt tgactcattt gaaccttcga ccttgtttgc
      121 cttattcaag gcccaaaggg aaaaaggggc tattatcaaa aaatgagttt ctctatgacg
      181 agtattttga ttgttatatt tgtccgcaag atcaaatgct cgctttttca acggttactc
      241 gagaagggta ccgagaatac aaatccaatc caaaagaatg tgtgaactgt c
//
LOCUS       DACDXO010000221          290 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E23605_2017
            SAMN12639989-rid9881463.denovo.237, whole genome shotgun sequence.
ACCESSION   DACDXO010000221 DACDXO010000000
VERSION     DACDXO010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639989
            Sequence Read Archive: SRR10023389
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 290)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 290)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741914.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:42:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,972
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 33 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 17 of 73
            Pseudo Genes (multiple problems)  :: 10 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..290
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639989-rid9881463.denovo.237"
                     /isolate="E23605_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            19..>290
                     /locus_tag="IWM35_002895"
     CDS             19..>290
                     /locus_tag="IWM35_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP9920655.1"
                     /translation="MQGVIAMMSKNNTNGRNQFAMLTIDDLVPQDHLVRKIDAALDFE
                     FIYPIVEATYSDLGRPSIDPVILIKLVFIQYLFGIRSMRQTIKEVDT"
BASE COUNT           88 a           53 c           57 g           92 t
ORIGIN      
        1 atagatttat gaaaacgaat gcaaggagtg atagctatga tgtctaaaaa caatacgaat
       61 ggtagaaatc aatttgcgat gcttacgatt gatgatctag ttcctcaaga tcatctcgtt
      121 cgaaagattg atgccgcgct tgattttgaa tttatttatc caatcgtcga agccacttac
      181 tccgatttag gtcgcccaag tatcgatcca gtgattctga tcaaattggt gtttattcag
      241 tatttattcg gtatccgttc catgcgacaa accattaagg aagtcgatac
//
LOCUS       DACDXO010000222          283 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E23605_2017
            SAMN12639989-rid9881463.denovo.238, whole genome shotgun sequence.
ACCESSION   DACDXO010000222 DACDXO010000000
VERSION     DACDXO010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639989
            Sequence Read Archive: SRR10023389
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741914.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:42:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,972
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 33 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 17 of 73
            Pseudo Genes (multiple problems)  :: 10 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..283
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639989-rid9881463.denovo.238"
                     /isolate="E23605_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            1..283
                     /locus_tag="IWM35_002896"
                     /pseudo
     CDS             1..283
                     /locus_tag="IWM35_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
BASE COUNT          109 a           57 c           56 g           61 t
ORIGIN      
        1 aacaaatcgt cgatctctat ctcgctggta agcctcgcgc agaaattatt cgagagtatg
       61 agcttacgcc ttcttctttc gataaatgga tgaagcaagc acaatcaacg ggctcattca
      121 aagaaagaga caacttaaca ccagaacaag cagaattgat cgcactaaga aagaaaaata
      181 agcaactcga aatggagaat gatattttaa agcaagcggc gctgatattc ggacgaaaag
      241 acaagtaatt gatgccaaca agcataaata ttccatatca gcg
//
LOCUS       DACDXO010000223        21240 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E23605_2017
            SAMN12639989-rid9881463.guided.048, whole genome shotgun sequence.
ACCESSION   DACDXO010000223 DACDXO010000000
VERSION     DACDXO010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639989
            Sequence Read Archive: SRR10023389
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21240)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21240)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741914.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:42:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,972
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 33 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 17 of 73
            Pseudo Genes (multiple problems)  :: 10 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21240
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639989-rid9881463.guided.048"
                     /isolate="E23605_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            320..2965
                     /locus_tag="IWM35_002897"
     CDS             320..2965
                     /locus_tag="IWM35_002897"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HAP9920656.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            3275..4588
                     /locus_tag="IWM35_002898"
     CDS             3275..4588
                     /locus_tag="IWM35_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HAP9920657.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            4578..5231
                     /locus_tag="IWM35_002899"
     CDS             4578..5231
                     /locus_tag="IWM35_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP9920658.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDLEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            5286..5837
                     /gene="radC"
                     /locus_tag="IWM35_002900"
                     /pseudo
     CDS             5286..5837
                     /gene="radC"
                     /locus_tag="IWM35_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736077.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
     gene            5968..7053
                     /locus_tag="IWM35_002901"
     CDS             5968..7053
                     /locus_tag="IWM35_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP9920659.1"
                     /translation="MKVQRIEVENKPYPLYLLLDKEYQLIEPVMKFIKYLDNTGKSPN
                     TIKAYCYHLKLLYEFMEQRGVILNDINFELLADFVGWLRYPSASNVIDLQSKKAIREE
                     TTVNTILNVVMSFLDYLSRLGEFKSIDVFKQAKGRNFKGFLHHVNKGRYQKNVLKLRV
                     KKKQIRTLRSEEVKQIIDACHTKRDKLILMLMYEGGLRIGEVLSLRLEDIVTWDNQIH
                     LTPRDVNVNEAYIKLRKERTIHVSKELMSLYTDYLIYEYSEELEHDYVFISLKEGYFG
                     KPLKYQSVLDLVRRIVKRTGIEFTSHMLRHTHATQLIREGWDVAFVQKRLGHAHVQTT
                     LNTYVHLSDQDMKNEFNKYLERKEHKK"
     gene            7050..8942
                     /locus_tag="IWM35_002902"
     CDS             7050..8942
                     /locus_tag="IWM35_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001833801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP9920660.1"
                     /translation="MNASSKRKIISQSEISKKIAVMNEEMQGFWANNSWDIRKCPHPS
                     AIELSKNPALRNRWVRFERVKNLWLRTELKYFYFYHLNNGIWNAKTVWIRKGTVINKM
                     LDFLDLKYPSITSITEVPIEKAMTEYRTYLTKRGVRITTTNYKITANQEKTPVKANSY
                     YVTNLKQFMEFYENFYFDGEEWDKDVWDRRNLPLPDDKVNPTQYEYTINFKGFRNTYF
                     KQLVKRYCKLRLNMDSFSYVSDIAQRLKEFFNFLDMKFKQVQRVHQLTRVEIEAYLSE
                     LNMMGIKPSTITGRISILEGLFSTLLRLEWDDVPSKILIYSEDYPKIPRAKPRFIDEF
                     VLEQLNSHLDKLPEYIATMTMIVQECGMRISELCTLKKGCLLEDKDGDFFLKYYQWKM
                     KKEHIVPISKEVALLIKVREDKVSEEFPDSEYLFPRKDGSPLKQETFRGELNKLAYEQ
                     NIVDKSGEIYRFHAHAFRHTVGTRMINNGMPQHIVQKFLGHESPEMTSRYAHIFDETL
                     KNEFTKFQEKLVTNNGDVLDLDEDNEVDDVELQWFKKNINAQVLPNGYCRLPVVAGGC
                     PHANACLDCTHFCTSKQFLPQHEEQLERTEELLAIAKDKQWQRQVETNSRVKERLEQI
                     IGSLTG"
     gene            8949..9326
                     /locus_tag="IWM35_002903"
     CDS             8949..9326
                     /locus_tag="IWM35_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP9920661.1"
                     /translation="MDKQVRNTTEIVRLAKQKSKKTREKVDKAISKFSIEGKVINFNS
                     IAKEANVSKSWLYKEHDIRQRIESLRERQITANVVSKPKKSSRSEEILIKTLKRRVME
                     LEKENKKLQNQIQKLYGDLYNKE"
     gene            9480..10262
                     /locus_tag="IWM35_002904"
     CDS             9480..10262
                     /locus_tag="IWM35_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(9)-Ia"
                     /protein_id="HAP9920662.1"
                     /translation="MSNLINGKIPNQAIQTLKIVKDLFGSSIVGVYLFGSAVNGGLRI
                     NSDVDVLVVVNHSLPQLTRKKLTERLMTISGKIGNTDSVRPLEVTVINRSEVVPWQYP
                     PKREFIYGEWLRGEFENGQIQEPSYDPDLAIVLAQARKNSISLFGPDSSSILVSVPLT
                     DIRRAIKDSLPELIEGIKGDERNVILTLARMWQTVTTGEITSKDVAAEWAIPLLPKEH
                     VTLLDIARKGYRGECDDKWEGLYSKVKALVKYMKNSIETSLN"
     gene            complement(10388..11119)
                     /gene="erm(A)"
                     /locus_tag="IWM35_002905"
     CDS             complement(10388..11119)
                     /gene="erm(A)"
                     /locus_tag="IWM35_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001072197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(A)"
                     /protein_id="HAP9920663.1"
                     /translation="MNQKNPKDTQNFITSKKHVKEILNHTNISKQDNVIEIGSGKGHF
                     TKELVKMSRSVTAIEIDGGLCQVTKEAVNPSENIKVIQTDILKFSFPKHINYKIYGNI
                     PYNISTDIVKRITFESQAKYSYLIVEKGFAKRLQNLQRALGLLLMVEMDIKMLKKVPP
                     LYFHPKPSVDSVLIVLERHQPLISKKDYKKYRSFVYKWVNREYRVLFTKNQFRQALKH
                     ANVTNINKLSKEQFLSIFNSYKLFH"
     gene            complement(11177..11264)
                     /locus_tag="IWM35_002906"
                     /pseudo
     CDS             complement(11177..11264)
                     /locus_tag="IWM35_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771940.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ErmCL family antibiotic resistance leader
                     peptide"
     gene            11628..12290
                     /locus_tag="IWM35_002907"
     CDS             11628..12290
                     /locus_tag="IWM35_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAP9920664.1"
                     /translation="MNTIESQFDKVAEDYDFVNELLNDYSFFVSNMSPKKGRALDIGC
                     GSGLLVEKLASYYDEVVGIDISNQMLDLAKSKRQLTNTVYLNMNAEQLNFNEKFDFIV
                     SRTTFHHLDDIASVIQQMKELLNEEGRIVILDNVSEVETPPTYVYKLGAIQEFLPHCF
                     KFGIKNAIRIYNHNTSKSWLEHLASDKYLSEQNYYDLYEKLLPGCQFHKMGWAMGVVW
                     TK"
     gene            12525..12668
                     /locus_tag="IWM35_002908"
                     /pseudo
     CDS             12525..12668
                     /locus_tag="IWM35_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein"
     gene            12818..13018
                     /locus_tag="IWM35_002909"
     CDS             12818..13018
                     /locus_tag="IWM35_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP9920665.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
     gene            complement(13272..14951)
                     /locus_tag="IWM35_002910"
     CDS             complement(13272..14951)
                     /locus_tag="IWM35_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HAP9920666.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
     gene            complement(14953..15165)
                     /locus_tag="IWM35_002911"
     CDS             complement(14953..15165)
                     /locus_tag="IWM35_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HAP9920667.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            15558..17288
                     /gene="efrA"
                     /locus_tag="IWM35_002912"
     CDS             15558..17288
                     /gene="efrA"
                     /locus_tag="IWM35_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HAP9920668.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            17285..19045
                     /gene="efrB"
                     /locus_tag="IWM35_002913"
     CDS             17285..19045
                     /gene="efrB"
                     /locus_tag="IWM35_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HAP9920669.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            19140..19337
                     /locus_tag="IWM35_002914"
     CDS             19140..19337
                     /locus_tag="IWM35_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9920670.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            19490..19975
                     /locus_tag="IWM35_002915"
     CDS             19490..19975
                     /locus_tag="IWM35_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HAP9920671.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(20079..20672)
                     /gene="clpP"
                     /locus_tag="IWM35_002916"
     CDS             complement(20079..20672)
                     /gene="clpP"
                     /locus_tag="IWM35_002916"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HAP9920672.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            20852..>21240
                     /locus_tag="IWM35_002917"
     CDS             20852..>21240
                     /locus_tag="IWM35_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9920673.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGG"
BASE COUNT         7027 a         3404 c         4423 g         6386 t
ORIGIN      
        1 aagtggtttt ttcacgacca cttgttgact aaaaaaaaaa accattgtag aatgaagcat
       61 aacgctgaac aagaggagta actggcacat tcattcttag agagagaact atctgctgaa
      121 aggttctcaa tgacaaccag tgaaggtagc ttgggagttt ctgtcttgaa tggagtagag
      181 atagacgagt cgtgatcgtt aactcacttg agaggcaata atgattgatt attgcaattt
      241 aggtggtacc gcgtaaatca tacgtcctag aatacgaaag tattttagga cgttttttag
      301 tttaaaggag gaaaacagca tgacggaaga aaataaccta tcaacaaaat ataatccgca
      361 agaagttgaa gctggacgtt atcagcattg gttagaacaa gatttgttta aaccgagcgg
      421 agataaaaaa gcaaaacctt attcaatcgt aatcccgccc ccaaatgtta ctggaaaact
      481 tcatttaggg catgcttggg atacgacctt gcaagatatg ctgattcgtc aaaagagaat
      541 gcaaggatat gatactttat ggctgcctgg tatggaccat gcaggaatcg cgacacaagc
      601 aaaagtagaa gcgaagctta gagagcaagg gatttctcgt tatgatctag gtcgtgaaaa
      661 gttcatcgat caagtgtggg aatggaaaga agagtatgcg agccacatcc gcgaacagtg
      721 ggctaaaatg ggcttatctt tagattacag tagggaacgt tttacattag atgatggatt
      781 atctgaagca gtacgtaaag tttttgttac cttatacgaa aaaggtttga tttatcgtgg
      841 tgaatatatc atcaactggg atcctcaagc acgaacagct ctatctgata tcgaagttat
      901 tcataaagat atcgaaggcg ccttttatca tatgagctat gaactagcag atggatcagg
      961 cgtagtagaa attgcgacaa cccgtccaga aacgatgttg ggagatacgg cgattgctgt
     1021 tcatccagaa gacgagcgtt atcaagcgct catcggcaaa aaagtgattc tacctttagt
     1081 caataaacaa atcccaatca ttgcagatga atatgtagat atggaattcg ggacaggtgt
     1141 agtaaaaatc actccagcac atgatccaaa cgactttgag gtaggtaatc gtcatgatct
     1201 tccacgcgta aatgttatgg atgatgatgg tacgatgaat gaactagcag gaaaatatgc
     1261 tggaatggaa cgttttgctg ctcgtaaagc aatcgtgcaa gatctgaaag aaatcggtcg
     1321 attgattaaa atcgatacga tgatccacag tgtgggtcat tccgagcgga caggtgtcgt
     1381 tgttgaacct cggctgtcta ctcaatggtt tgtaaaaatg gcgccgttag cgaaaaaagc
     1441 cattgataac caagaaacag atcaagcagt ggaattcttc cctccaagat tcaatcaaac
     1501 gtttttacgt tggatggaaa atgtccatga ttgggtaatc tcacgacaat tatggtgggg
     1561 acatcaaatt cctgcatggt atcacaaaga aacaggtgaa atgtatgtag ggatggaagc
     1621 accagcggat agtgaaaact gggtacaaga tcctgatgtt ttagacactt ggtttagttc
     1681 agctttgtgg cctttctcaa caatgggctg gccagatacg gaaagcgaag attataagcg
     1741 atacttccct actagcactt tagtaaccgg ttatgacatc attttcttct gggtaagcag
     1801 aatgattttc caaagcttgg aatttactgg cgaacgtcca tttgagaatg tcttgattca
     1861 tggattgatt cgtgacgaac aagggcggaa gatgagtaaa tcattaggta atggaatcga
     1921 tccgatggaa gtaatcgaaa agtatggagc agatgctctc cgctggttct tatcgaatgg
     1981 ttctgcacca ggacaagatg tccgcttcag ctatgaaaaa atggatgctt cttggaactt
     2041 tatcaacaaa atctggaatg cttcacgttt cgttatcatg aatatcgaag gaatgaccgt
     2101 agaagagatc gatttatctg gagaaaaatc agtggctgac cggtggatcc ttactcgttt
     2161 gaatgaaacg atcgaacgag taactgagct atttgatcgt tttgaatttg gtgaagcagg
     2221 tcgacaactg tataacttta tctgggatga tttttgtgac tggtacatcg aaatgagtaa
     2281 agaagtactt tatggagatg atgctgtttc aaaacaaacc acccgtagca tcttagtcca
     2341 tacacttgat cagatcctac gtttattgca tccaatcatg ccattcgtta cagaagaaat
     2401 atgggaacat attcctcatc agggaaattc tctagtagtg gcagaatatc ctgttgtacg
     2461 tccagagttt gatgatgaaa cagcttctaa agggatggaa gtattgaaag aattgattcg
     2521 agccgttcga aatattcgtt cagaagtgaa tacaccattg tctaaaccaa ttacgttgct
     2581 aattaaaaca aacgatgcta aaatcgatca atttttagtt gaaaatacaa gctatatcga
     2641 acgcttctgt aatcccgaag agttgacgat atcaagtgag atcactgcgc cagatctagc
     2701 gatgtcagct gttctgacag gtgcagaaat cttcttgccg ttagcaggat tgatcaatat
     2761 tgaagaagaa atcaaacgat tagaaaaaga actggaaaag tggacaagcg aagtaaaacg
     2821 tgtccaagga aaactttcta atgaacgttt tgtttctaac gcgccggaag aagtagtaga
     2881 ggcagagcgt gcaaaagaaa aagattattt agagaagcaa acggctgtca aagaacgaat
     2941 cgaaagtttg cgtactattc aataagaata gaaaaagaga gtgtgaaatc agctagctat
     3001 tactaaaagc tagctgattg aacatcgtta caggaaagag tttgagcctc actcttagct
     3061 tcgattcata aggagatgtg acataaattt tgtcgcatct cttttttagt ctactcttat
     3121 tatgtaggag aaggagggag gtggatcatg tatcaagagt caataaagta gttgataaaa
     3181 tcgttcctct ctctgtttct tttctctttg tcatactttt atttttctta tgtgttctat
     3241 ttgttataat aaaagagaaa tgggggaatt gttgatgacg attgaagaag caatagaatg
     3301 gatccatagt cgattaccat ttggttcacg tcctggcctt gaccgagtcg aagctttatt
     3361 agaagtggta gggcatccag agagaaaagt aaaaacgatc catatcgctg gaacaaacgg
     3421 aaaaggttct actgtagctt atttgagaag cctattagaa gaggctgggc tgacagtagg
     3481 tacatttact tcaccttata tcgaaacgtt caatgaacgg atcgcaatca atggcaaaag
     3541 cattccagac aatgatttga tccagcttgt aaaaaaatat cagccactga tcaaacaact
     3601 tgatcaattt gaagcagtag ctggtattac tgaattcgag acattaacag caatggcatt
     3661 tgattatttt ttgatgaaga aagtggacgt agcgattatc gaagttggct taggcggctt
     3721 attggattct accaatgtag ctgtaccaat gctgacggga ataacaacga ttggacttga
     3781 tcataccgaa atacttggag atacaattga agaaatcgct gcacaaaaag ctggaatcat
     3841 caaacaaggc gttccagtag taacaggaaa tattgcccca gaagctttta ctgtcatcca
     3901 acaaacagct aaagcaaaaa atgcaccgct ttatgtatac ggggaaaatt ataaaataaa
     3961 ctatctgcat cctgatgaga tgtggggaga agtatttgaa ttttttaatg aaataggaaa
     4021 gatccctcaa ttaaccactc cgcttttagg gcaacatcaa gctgaaaatg caggagtagc
     4081 catcgaattg tattttgttt actgtcaatt ggaaaagctg cctttccgag aaaaagatgt
     4141 tttgaaggga ttgaagaaat ctcgatggcc aggaagaatg gagcgtttga gtaacgagcc
     4201 attagttgtt ttagatggtg cccataatac gcatgcaatg aaccggcttg ttgagaattt
     4261 gaaaaaagaa ttcaaaggat atcaaatcaa cattttgttt tccgccttac gaactaaaaa
     4321 tgttagagaa atgctagaac gattgcagca agtaccaaat gtccatattt atttgacgac
     4381 ctttgaacat ccaaaagcca tcgaattaag tgaagtatca gatttagcaa gtgagagagt
     4441 atcaatcgtt tcattgtggc aattcggatt agcagatata ttggaaaaga tgacaaatga
     4501 agacgtatta gttgtgaccg gttcacttta tttcatttca gaagttcgcc atttattaat
     4561 acaattagga ggagatcatg aagtataatg cagtgatttt tgacatggat ggtttgctgt
     4621 tcgacacaga gttagtgtat tacgaagcca cacaagtagt agcagacaag atgggttttc
     4681 cttatgataa agacctgtat ttaaaattct taggtgtttc agatgaagaa gtatgggcga
     4741 attatcatat gattttctcg gaatttggca aaacaagtgt acaaagattc atagatgatt
     4801 cataccagga aacgcttgaa cgtttttcgc aaggagcggt acaaatgaag cccggtgtgt
     4861 tggaactcct ctctttttta gaagaacatc agattcccaa agttgtcgct tccagtaacc
     4921 aacgagcagt cattgaactt ttattgaaga agaacaatct gtttgaacga tttgagacga
     4981 ttgtttctgc agaaaatgtt caacgagcaa aaccagacct agaaattttc ttgttagcta
     5041 atcagaagct aggtacagcg aagaaagaaa cattgatttt agaagattca caaaatggta
     5101 ttttagcagc ttctgcggct gagatcccgg tagtcatggt accagatctg cttccaccat
     5161 cccaagaatt gatcaaaaaa acagctgggg ttgtttcttc tttacatgaa atccctgcat
     5221 tgttaaaata aaaatccatc tcttttcgta tcttatttta gataagatag aaaggagatg
     5281 tttttttggg aaaacgattg atcaaagaag tacctactag ctctttacca agggaacgta
     5341 tggaaattta tggcgcggaa gctttatcgg atcaggagct tttggcaatc ttactgcgta
     5401 ccggtcaaca cccttatagt gtgatgtcga ttgctggaaa tctgttaaaa acgtttggag
     5461 gactggcctc tttacgacag gctactttgc atgagttaga agagattcga gggataggac
     5521 gcgtgaaagc aatcgaaatc aaagccttga tcgaattagg tcgacgaatt cagacggatc
     5581 atgaaagagc ggctccaccg gttcgtacca gctacgaatt ggcccagcaa ttgattctgg
     5641 aaatgaaaga tcacaaacaa gaacatttgg tttgtattta tttggacacg aagaatcaag
     5701 tgatcttaaa aaaaacagtg ttcatcggtt cgctgaatca gtcagtcgct catcctagag
     5761 aaattttcca ttatgctgta cgttattgtg cagcaaggat cgttttagcg cataatcatc
     5821 cttctggtaa tgttttaacc atagtataat aaataatagt agttaaagta gtttaagagg
     5881 agatattaaa gggatatttt actttaaata aaaattaaca aggtttataa tttgtagtag
     5941 ttaaagtatt taaagaggtg ggaacatgtg aaggttcaaa ggatagaagt ggagaataag
     6001 ccgtatccat tgtatttatt actagataaa gaataccagc taatagaacc agtaatgaaa
     6061 tttattaaat acttagataa tactggtaag tctcctaata ccattaaagc atactgctat
     6121 catttaaagt tgctgtacga gttcatggaa cagagaggtg ttattcttaa tgatattaac
     6181 tttgagttgt tagcagactt cgtaggttgg ttgagatatc cttcagcatc aaatgtaatt
     6241 gatcttcagt caaaaaaagc cataagagaa gaaacgacag tgaatacaat tttaaatgta
     6301 gttatgagtt ttcttgatta tttaagtaga ttaggagaat ttaaatcaat tgatgtattt
     6361 aaacaagcaa agggaagaaa tttcaaagga tttttacatc atgttaataa gggtagatac
     6421 caaaagaatg tcttaaagtt aagggttaaa aagaaacaga taagaacatt gagatcagag
     6481 gaagttaagc aaattattga tgcttgtcat acgaaaagag ataaattaat tttaatgctt
     6541 atgtatgagg gtggtttaag aatcggtgaa gtgttatcgc ttaggcttga agatattgtc
     6601 acttgggaca atcaaatcca tttaacacct agagatgtta atgttaatga agcttatatt
     6661 aaattaagga aggaaagaac aatacatgtg agtaaagaac ttatgtcact ttatacagat
     6721 tacttgatat atgagtatag tgaggaattg gagcatgatt atgtttttat ttccttaaaa
     6781 gaaggctatt ttgggaaacc actaaagtac caaagtgttc ttgatctagt tagaagaata
     6841 gttaaaagga ctggaataga atttacatca catatgcttc gccacactca cgcaacgcag
     6901 ctaattaggg aaggatggga tgttgcgttc gttcaaaaga gattaggtca cgcacatgtt
     6961 cagacaacgt taaataccta tgttcatctt tcagatcagg atatgaaaaa tgagtttaat
     7021 aaatacctcg agagaaagga gcataagaaa tgaatgcttc tagtaaaagg aaaattatta
     7081 gtcagagtga gattagcaaa aaaatagctg taatgaatga agaaatgcag gggttttggg
     7141 ctaataatag ttgggatata agaaaatgtc cacatccttc tgccatagaa ttaagtaaga
     7201 atcctgcttt aaggaatcgt tgggttcgtt ttgaacgtgt taaaaatctg tggttaagaa
     7261 cagaattgaa atatttttat ttttaccatt taaacaatgg aatatggaat gcaaaaactg
     7321 tctggattag aaaaggaaca gtaattaata aaatgttaga tttcttagat ttaaagtatc
     7381 ctagcattac ttcaattact gaagttccta ttgaaaaagc aatgacggag tatagaactt
     7441 atttgacaaa acggggtgtt agaattacca ctactaatta taagattact gctaatcaag
     7501 aaaaaacacc tgtaaaagct aattcctact atgttactaa tctaaaacaa tttatggagt
     7561 tttatgagaa cttttatttt gatggagagg agtgggataa agacgtttgg gatagacgta
     7621 acttaccttt gccagatgat aaggttaacc caacacaata tgaatataca attaacttta
     7681 aagggtttcg gaatacatat tttaaacaac ttgtaaaaag atattgtaag ttgagattga
     7741 acatggatag cttttcctat gtaagtgata ttgcccaaag acttaaagag ttctttaatt
     7801 ttctggacat gaaatttaaa caagttcaga gagtacacca attaacgaga gtggaaattg
     7861 aagcatattt aagtgaacta aacatgatgg gaataaaacc tagtacaata actgggagga
     7921 tctctatatt ggaaggacta tttagtaccc ttcttaggct agaatgggat gatgttcctt
     7981 ccaaaatatt aatttattct gaggactatc cgaaaatacc aagagcaaaa ccacgcttta
     8041 tagatgaatt cgtcctagag caattgaaca gtcatcttga taaattaccc gaatatatag
     8101 ctacgatgac tatgattgtt caagaatgtg gaatgaggat aagtgaattg tgcaccttga
     8161 aaaaaggctg tctattagag gacaaagatg gagatttctt tttaaagtat tatcaatgga
     8221 aaatgaaaaa ggagcatata gttccaatat ctaaagaggt agctttactt attaaagttc
     8281 gggaagataa agtttcagag gaatttccag atagtgaata cctctttcca agaaaagatg
     8341 gatcgccatt aaaacaagaa acatttagag gtgagttaaa taaattagct tatgagcaaa
     8401 atatagtgga taaatcaggt gagatttata gattccatgc ccatgccttt cgccatacag
     8461 taggaacaag aatgattaac aacgggatgc cccagcatat tgtgcagaaa tttttggggc
     8521 atgaaagccc agaaatgaca agcagatacg ctcatatctt tgatgaaact ctaaaaaatg
     8581 aatttactaa atttcaggaa aaactggtta ccaataatgg agatgtgctt gatctagatg
     8641 aagataatga agtcgatgat gtagagcttc aatggttcaa gaaaaatata aatgcacaag
     8701 tgcttccaaa tggttattgt agattgccag tagtagcagg tggttgtcca catgcgaatg
     8761 catgcttaga ttgcactcac ttctgtacca gtaagcaatt cttaccacag cacgaagaac
     8821 agttagagcg tacagaagag ttattagcca tagctaagga taaacaatgg caaagacaag
     8881 tagagactaa tagccgtgtt aaagagcgtt tagaacaaat cattggaagt ttgacggggt
     8941 aattatcaat ggataaacaa gttagaaata caacagaaat tgtacgtttg gcgaagcaga
     9001 aatcaaaaaa gacaagggaa aaagtagaca aagcgatttc taaattttcg attgaaggta
     9061 aagttattaa ttttaattca atagcaaagg aagctaatgt ttctaaatca tggctttata
     9121 aggaacacga tattaggcaa agaatcgaat cccttcgtga gcgtcaaata acagcaaatg
     9181 tagtctcaaa acccaagaaa agttctcgtt cggaggaaat ccttattaaa accttaaaaa
     9241 gaagagtaat ggaattagaa aaagaaaata aaaaattaca gaaccaaatt caaaaattat
     9301 atggagatct gtataataaa gaataattat taatctgtag acaaattgtg aaaggatgta
     9361 cttaaacgct aacggtcagc tttattgaac agtcatttag gtatatgtcc aatctagggt
     9421 aagtaaattg agtatcaata taaactttat atgaacataa tcagttacga ggtgaaatca
     9481 tgagcaattt gattaacgga aaaataccaa atcaagcgat tcaaacatta aaaatcgtaa
     9541 aagatttatt tggaagttca atagttggag tatatctatt tggttcagca gtaaatggtg
     9601 gtttacgcat taacagcgat gtagatgttc tagtcgtcgt gaatcatagt ttacctcaat
     9661 taactcgaaa aaaactaaca gaaagactaa tgactatatc aggaaagatt ggaaatacgg
     9721 attctgttag accacttgaa gttacggtta taaataggag tgaagttgtc ccttggcaat
     9781 atcctccaaa aagagaattt atatacggtg agtggctcag gggtgaattt gagaatggac
     9841 aaattcagga accaagctat gatcctgatt tggctattgt tttagcacaa gcaagaaaga
     9901 atagtatttc tctatttggt cctgattctt caagtatact tgtctccgta cctttgacag
     9961 atattcgaag agcaattaag gattctttgc cagaactaat tgaggggata aaaggtgatg
    10021 agcgtaatgt aattttaacc ctagctcgaa tgtggcaaac agtgactact ggtgaaatta
    10081 cctcgaaaga tgtcgctgca gaatgggcta tacctctttt acctaaagag catgtaactt
    10141 tactggatat agctagaaaa ggctatcggg gagagtgtga tgataagtgg gaaggactat
    10201 attcaaaggt gaaagcactc gttaagtata tgaaaaattc tatagaaact tctctcaatt
    10261 aggctaattt tattgcaata acaggtgctt acttttaaaa ctactgattt attgataaat
    10321 attgaacaat ttttgggaag aataaagcgt cctcttgtga aattagagaa cgctttatta
    10381 ctttaattta gtgaaacaat ttgtaactat tgaaaataga aagaaattgt tccttcgata
    10441 gtttattaat attagtgaca tttgcatgct tcaaagcctg tcggaattgg tttttagtga
    10501 aaagaacacg atattcacgg tttacccact tataaacaaa agatcgatac tttttgtagt
    10561 ccttctttga aatcaatggt tgatgtcgtt caagaacaat caatacagag tctacacttg
    10621 gcttaggatg aaaatatagt ggtggtactt ttttgagcat ttttatatcc atctccacca
    10681 ttaatagtaa acccaaagct cgttgcagat tttgcaatct tttcgcaaat cccttctcaa
    10741 cgataagata gctatattta gcctgacttt caaaggtaat tcttttgaca atatccgtac
    10801 tgatgttata aggaatatta ccatatatct tatagtttat atgttttggg aaggaaaatt
    10861 ttagaatatc cgtttgaatc acttttatat tctcagaggg gtttaccgct tctttagtca
    10921 cttgacataa gcctccatca atttctatag cagtaactga tcgactcatt ttgactagct
    10981 ctttggtaaa atgtcctttt cctgatccga tttctattac gttgtcttgt ttactgatat
    11041 tcgtgtgatt caatatttct tttacatgct ttttagaagt aataaaattt tgcgtgtctt
    11101 tagggttttt ctggttcatt ataaccttct ccttactggt tataatgaac tgacattaag
    11161 cgttcattat aaccaattaa ttttgatttg gttgataacg aactttatta ataacaaata
    11221 tagaaaaagt acccatactt ttatctcctt tccatttttt tcattatgaa tgagataaag
    11281 taatttctac tactgcgata ctggtgcaca taattgaaac ctccttatgt aactgaatat
    11341 gattgtatca tcttttcatt ttagaataaa cctttatatg taattttgca cttgacactt
    11401 cagatggacg ttttcttcaa aataatatgt tttgaaactt gataagagaa gccactgtct
    11461 ttactaaaaa gtaaaggctg cgataaagga cagtgacttt aataaagttg cactgttgta
    11521 ttgtctttgg ataaaattgt ctaaagggaa gttttatccc caacgaaaaa acatctaaag
    11581 tggtaagtaa tgaaagaatg tcgataaagt aggaatgggg gatctttttg aatacaatag
    11641 aatcacagtt tgataaggtt gcagaagatt acgatttcgt gaatgagctt ttgaatgatt
    11701 attcattctt tgtgtctaat atgtctccaa agaaaggcag agcattagat atcggatgtg
    11761 gctcgggttt gttagtggag aaattagcaa gttattatga tgaagtggta gggattgata
    11821 tttctaatca aatgctcgat cttgccaaat ctaaacgtca actaacaaat acggtctatc
    11881 tgaatatgaa tgcagaacaa cttaatttta atgagaagtt tgattttatt gtaagccgaa
    11941 caacctttca tcatttggat gatatagcca gcgtaataca gcaaatgaag gaactgttga
    12001 atgaggaagg aagaatagtt attcttgata atgtatctga agtcgagaca ccgcctacct
    12061 atgtttataa attaggggca atccaagagt ttttaccgca ttgtttcaaa tttggaataa
    12121 aaaatgcgat tagaatttat aatcacaaca catctaaatc atggcttgag catttagctt
    12181 cagataagta cctatctgaa caaaattatt acgatttata tgaaaagttg ttgcctggat
    12241 gccaattcca taaaatgggt tgggcaatgg gggttgtctg gacaaaataa cttaaagctg
    12301 tggctttgaa aagttgaatt tgtgattgtt ttcagagcta tcgggtgctt tagtataaat
    12361 aacaatgaat aaaaaatcag cctgtatctt atgttactat taagtaattt aagatacagg
    12421 ttttactatt tttgaaataa gtaccttcca cttcaaatac atgtattcaa agtgaaagta
    12481 ttgatttaaa tgattctttt gtaatttact cttgataata tgaaattcca tcgcagcagg
    12541 atatgaattt taccaaaagg atacaaaagt gtggagaaat gatggggatc actgtactgg
    12601 accacttgat catcggacgc aaacgatatt ttagtttgag agaagaagga atgatggaag
    12661 agaagtaagt tgctttcttg caaaaaaaat gaagcaagtg tacaattata ttgtaatttt
    12721 gtttgggaat agaagttgaa acatctgttg cacctctatc gtacacgagc aatgttacaa
    12781 tttaaattag tgcttttggc acgaggagga tattattatg gaaaacggaa cagtaaaatg
    12841 gttcaacgct gaaaaaggat ttggatttat ctcacgtgaa gatggaagcg acgtattcgt
    12901 tcacttctca gctatccaag gtgacggatt caaaacatta gaagaaggtc aagcagtgac
    12961 tttcgacgtt gaagaatcag accgcggccc acaagctgca aacgttgtaa aagcttaatt
    13021 ttatctgtaa atcaaattga tggtagacat caatttttac gagagaacaa agcgagtaac
    13081 aaaaatacct gccacaagag acaatttctc ttgtggcagg tatttttttg tatgaaaaag
    13141 taaataaatt taagaggcta tgaaagagcc accttgtatc aaggaataag aacaaacaaa
    13201 aatggcttct catgtttttc atttgttttc tcttatattc aggacaaggt gctgatttca
    13261 taacctctta tttattcttc atctggtgtc attaccattg gcaaaatcat tggatgtctt
    13321 tctgtacgtt cgaataagaa aggctgaagc gcgccagtca tcgcttcttt caatttatat
    13381 tctgaacagt caggtccatt caaacattct cttaaagcgt ggaataagat tcgctgagct
    13441 tcattgatca tttctcctga ttctcgcatg tagacaaatc cacgagagag aatatctgga
    13501 ccagccaaga tttctttttt cttgatatcc actgtcgcga cagctaatac aagaccttct
    13561 tctgaaagca ggtgacggtc acgtaaaacg acattaccaa tatcaccgat cccgctacca
    13621 tcgatataca catctccagc cacaaaatgc ccagcaggac gcgcagaatc gctagttaac
    13681 gctaaaacat cgccattttc catgatgaag cagttttctg caggaacacc tacatcttgt
    13741 gctagagaag catgtacttt caacatgcgg aattctccgt ggacaggaat aaaatatttt
    13801 ggcttcatca aacgaagcat taatttttgt tcttcttgtc caccgtgacc ggaagtatgg
    13861 atgttattga tttttccgtg aatcacttct gcaccagctt cagacagcaa attgatcaaa
    13921 tgattgacac ttgttgtatt accaggaata ggtgaacttg aaaagatcac tgtatcgtct
    13981 ggctggattt ggatttgacg gtgagtacca ttcgcgatgc ggctcaacgc ggccatcgct
    14041 tctccttgag aacctgtaca taggatcatc gtttcattgg caggcaagcg attgatttcg
    14101 tgtgcatcta caaatgtgcc gtctggaact ttgatatatc ctaaacgttg accgttgacg
    14161 atggcatttt ccatgcttcg tccgaaaaca gcgattttac gaccagttgc cacagcggca
    14221 tctgctgctt gctgcaaacg gaagatgtta gaagcaaagc tggcaaaaat gatacgtcct
    14281 tcgatttttg aaaagatctt caaaatagaa tgcccaatcg ttttttctga tttagtaaat
    14341 gttggaatct cagcatttgt actatcagat aaaaggcaca agactccttc ttccccgata
    14401 cgagccattt tatgcaagtt tgctggttcg ccaacaggag tgaaatcgaa tttgaagtct
    14461 ccagtcgcta cgatatttcc agaaggtgtt ttcaccacga tccctaatgc atctgggata
    14521 ctatgggtcg ttctgaaaaa gctgacagaa gtctttctaa aacggatgat cgtatcttca
    14581 ttgatctcat ggagctctgc atctcgaagc aagccatgct catctaattt gtttgtgatc
    14641 aacgctaatg cgagaggacc agcatagata ggaatatttg cttgtttcag caagtagggt
    14701 attccgccga tgtggtcttc gtgcccgtga gtaatcacca aggcttttac tttgtgtaag
    14761 ttttgtatga tgtagctgta gtcgggaatg acatagtcaa ttccaagtaa atcgtcttcc
    14821 gggaatttga tcccggcatc aatcaaaatg atttcatctt ggaattgtac cccatacgta
    14881 tttttcccga tttcacctaa tccaccaata gcaaagacgc ctgtctcgtt atttttgatg
    14941 gaaagtttca tattaaaact ccactaatga gaaatcagca ttttcttttt cgtactctag
    15001 atgacttcct tctagaagtt gaacgaattc aatattgtat ggggtatttt cttccacacg
    15061 ttggcgagcg tctacatcgc tttttgcctc aatgtaaaga gattttgttt cttcccgttt
    15121 agggttgcgt acttttgttt cttgataata aactttatag atcattgttt tgtctccttt
    15181 atctgcgtaa agcagtgatt tagctagttg atttttttag acaacacaaa acgaggggac
    15241 aagccacctg gtttttcata tgctttgact agaatactgg attcgagtca aagagacaag
    15301 cctcgttttt gcttatctgt tcggatattt tttcaaccgt accttctgta taaaaaatat
    15361 acaacaatta attgttattc tagcataaaa cagcaaataa gtatagaaat caactagatt
    15421 gaaagaaaaa atcaatgttt atcatcccgc tataatcata atgacttttt tatgagagaa
    15481 gtgttctatt aataggctcg ttgtgctatc atttttagta atgaaaatgt aacaaaagat
    15541 taaaaggaga tgagaaagtg aaactaatgt ggcattatac catgcgttat aagaaattct
    15601 tgtttttcaa tttcatatgc gtatttggtt ttattttgat tgaattagga cttcctacga
    15661 ttttagcacg tatgatcgat gtaggaatcc gcaatgatga ttttgattat gtgaagcaac
    15721 aagggatatt tatgatcgtt attactgtaa tcggtattat tttgaatatc ttgttaggtt
    15781 attttggttc gaggattacg actaatatcg tagcagatat tcgagatgat ttgttcgaaa
    15841 agatccaaag ttattcgcat caggaatacg aaaatctagg cgtatcttca ttgatcactc
    15901 gtacaacaaa cgatgcttat cagatcatgt tgttcctgca aaatatttta cgtatcggtt
    15961 ttatggcacc aatgatgttc attgtcagtt tgtacatggt catgcgtaca agtgtgacac
    16021 tcagtttgta tgtagtaggt gcattacctt tactattatt ggcagtcgta gcgattgcta
    16081 aggtgtctga accgttatct aaaaagcaac aaaaaaactt agataaaatc aacagtatct
    16141 tgcgggaaaa tctttcagga ttgcgtgtga ttcgtgcatt cgtcaatgaa aagtttgaag
    16201 aaaaacgttt tgctgtagtc aatgagaatt atgcttctag ttcaaaaagc ttattccgtt
    16261 tgatggctgt tgcacaaccg ggtttttatt tcttatttaa tatcgtaatg gtattgatca
    16321 tctggagcgg ggcagtgcaa atcgatgcag gcgagcttcg tgtaggagat ttgattgcgt
    16381 ttatcgagta tatattccat gccttgttct cattcatgtt atttgctagt gtgttcatga
    16441 tgtatccaag agcagctgtt tcagctggac gtatccaaga agccctagat gccaaacctt
    16501 cgattgtaga agatccaaat ggtatatctg atacaaaaac taaagggtat atcgaatttc
    16561 aaaatgtaac atttgcttat ccaggccatt cgcaagagcc agtgatacga aatgtcagtt
    16621 ttacagcctc tccaggagag accgttgctt ttattggaag taccggtagc ggaaaatcta
    16681 cgttgatcca attgatccct cgcttttatg atgtgagtga aggaaagatc ttgatcgatg
    16741 gagtagacgt gagagattat cggctaagca agttgcgaga taagattggg tttattccgc
    16801 aaaaagcgct gctatttact ggaacgattg cagataattt gcgatacggg aaagaagatg
    16861 ctacgcaaga agaaatggaa cgtgcagctg agattgctca agcttcagaa ttcatttctc
    16921 gaaaaccaga tggatttaat gaacatttat cagaaggtgg ttccaatttt tcaggtggac
    16981 aaaaacaacg attggcaata gccagagcca tcatacgccg tccggaaatc tatatttttg
    17041 atgacagctt ttctgcatta gattatcaaa cggatgcgaa gttacgtgct cgtctgaaaa
    17101 aagaaacgac agaatctact gtattgatcg tagcacaacg tgtgggaacg atcatgcatg
    17161 cagataagat catcgtatta aatgaaggag aagtcgtcgg tatggggacg catcgggagt
    17221 tgttggaaaa atgcccgatt tattatgata ttgccgcttc tcaattatca aaggaggaat
    17281 tagcatgaaa aaaacctttt cttccttaaa acgtttagct cgatatatca gaccgtatcg
    17341 tgagactttt cttttagtga tcctatttac agttctgaca gtggctttca acgtggcttt
    17401 gccatatgtt acaggacttc ctactacaga aatcagccgt aatattgcca atggcgaatc
    17461 aatcaatttt tcttatatat tcaaatgtct catttggatc acagttgttg gaatagggta
    17521 ttgtatttcg caattacttt ctggtgtttt gatgacaaat gtcgttcaaa gtgccatgca
    17581 tgatttgaga agagacatag aagaaaaaat caatcgtctt ccggtttcat atttcgataa
    17641 aaaccagcaa ggaaatattt tgtctcgtgt gacgaatgat gtagatgcag tcagcggtgc
    17701 tttacaacaa gcttttattg gtatcgtaaa tgcgatattg ggaattacgt tggctgcagc
    17761 aatgatgttt tatatccagc cgatgatggc gctgatctct atgatcatga ttccaacatc
    17821 gatctggatc tcaaaacgag tgatcaatgc ttcgcagaaa tattttcaaa gcatgcagaa
    17881 ttctcttggt gagctaaacg gctacgtcca agaaaatatg acaggtttta gtgtattgaa
    17941 agtatatgga cgcgaaaaag aaacattgga aggattcaaa aaagttaatc acagcttgaa
    18001 atactttggg tttcgtgcag cattcatttc tggcttgatg atgcctctag ttcaattaac
    18061 tgcctatgct acttatattg gtatggctgt tctaggaagc ttttatgtta ttactggggc
    18121 gatcgttgtc ggtcaactac aagcattcat ccaatatatt tggcaaatca gtcagccaat
    18181 ggggaacgtg acacaattgt cttcagtttt gcaaagtgca tcagcggcaa cgaaacgtgt
    18241 attcgagatt ttagatgagc cagaagaaaa agaaaacaaa gtagatgctt cactgccaga
    18301 aacgatttta ggagatgtga cattcgatca tgtcgatttt gcatatgatc cgaagaaacc
    18361 attaatccaa gatttgaatt ttgaagtgaa agctggtcaa accgtagctg tggtcggtcc
    18421 gaccggtgcc ggcaagacga cattgatcaa tctattgatg cgtttttatg acgtagacaa
    18481 aggagcaata aggattgatg gaatcgacac caaatcaatg agtcgcagtg atgtgcgttc
    18541 gctatttggt atggtcttgc aagatgcatg gctttatcaa ggaacgattg cagataatat
    18601 ccgcttcgga aagcttgatg cgacagatta tgaagtagtt gatgctgcga aaacagcaaa
    18661 cgtagatcat tttatccgaa cgatgccaga tggatatgat atggccatca attctgaggg
    18721 agacaatgtg tctttaggac aaaaacagtt gctgacgatt gcccgagcag tggtctccga
    18781 tcctaaaatc ttgattttag atgaagcaac aagttcagta gacacccgtt tagaagcgtt
    18841 gattcaaaaa gcaatggatc gcgtgatgga gggacggaca agtttcgtca tcgctcaccg
    18901 tttatcgaca atccgtgatg cagatctgat tctagtgatg gatcaaggtc agatcattga
    18961 aaaaggaacg catgagagtt tattggcaca aggtggattt tatgaaaaac tgtataacag
    19021 ccaatttgct gaggaagcag aataagtcat ataagaataa aacagtttaa aacttatttt
    19081 ttgttctcta ttcttgataa tggtaaagta aagttatcaa gaaaagtgag gacacggaga
    19141 tggaacagtt tttaaatgaa aaaattactg aagccaaaag agaagttgat cgtttaacgc
    19201 acgtctgtca gacgaagcct tttgattcag ctgatttttt tgaaaatgag tttcaaatcg
    19261 aacgcttggc agcacaaata gaagtgtacg aattaatttt aaaaaaagta atagaagaaa
    19321 atatagatgt acaataaaaa caggtaaaac gtcagttacc agatactgta aatagacatc
    19381 tggcctgagc aggttttacc tgtttttttt cttatgaaat aaatatatgc tatgatacct
    19441 ttattcaata ggattagttt ctacaaaaaa tgaaaggaga aaaataaata tggaacggtt
    19501 tatagcatca aatgccacag tgattggtga tgtgacattg tcagaagatg tgacgatctg
    19561 gtatcaagct gttttacgcg gggacagtaa ttggataaaa ataggacaaa gaacgaatat
    19621 ccaagatggt acaatcatcc atgtcgatca tgatgcacct gtagatattg cggagaatgt
    19681 gacggtagga catcagtgca tgctacatgg ctgtacaatc gaaaaagggg cgctgatcgg
    19741 gatggggacc acgatattga atcatgcggt tattggtgag aatagtttga ttggtgctgg
    19801 atcattagta acagaaggaa aagttattcc tcctaatgtc ttggcttttg gtcgcccagc
    19861 taaagtgatc cgtcctttga caaaagaaga aatacaaaaa aacaaagaaa acatccagca
    19921 ttatgtcgag ataggtcgga tgcatgcgca aggaaaattc aaggagcaaa gataggcagt
    19981 agcatccata aaataaaaaa ggatggtcag tttgagttga ccatcctttt ttatttggtt
    20041 tagataagag agcttaaaaa agcttccttt gaaacagatt aatttaatga agaactgttt
    20101 tccatgactt catcgatcaa tccatatgct tttgcttgtt cagctgtcat atagttgtca
    20161 cgatctgtat ctttttcaat cacttcaagc ggttgtccag tgcgttcagc taaaattttg
    20221 tttaagcgtt cgcgtgtttg caaaatatgg cgtgccgcaa tttcgatttc agttgcttgc
    20281 ccttgagcgc cgccaagagg ttgatggatc atgatctcag catttggtaa agcaaatcgt
    20341 ttaccttttg taccagctgt cagcaagaag cttcccattg aagcagccat acccataaca
    20401 attgtttgga catctgcttt gacaaagttc attgtgtcgt aaatagccat acctgctgta
    20461 actgaaccac caggagaatt gatataaaga tagatatctt tttcagaatc ttgagcgtct
    20521 aagaaaagca attgggcgat gatcgaatta gcaagatcat ctgttacttg gccgcttaac
    20581 atgatgatac ggtctttcaa taaacgtgag tagatatcgt aagctctttc acctcgtgaa
    20641 gattgttcaa tgactgtagg aattaaattc ataatagagt tcctccttga tttcgtttag
    20701 aaatagtgta ccataattta gtttctaata cctaaaatgt agtatacatt tttggtcaaa
    20761 aaaggtcaaa taaaaaactt aggcaaatta gtcgcataaa cccgctttat ggtatgataa
    20821 gcagagaaac aaaacaagga ggaacaacag attgatagtt ggtattactg gccttatcgg
    20881 agtgctgaca ggtgctgtag ttgtctatat cggcatgata ctgcaaatgc gtcacttgga
    20941 aaataaagaa cagcgtagac gtgaattgga attgaaagtc aaagaaatcg agacgctgaa
    21001 tcaattaaat aaaaagatca acgagatctt gcaaaaacgg acaacgctga tggaagaata
    21061 tgtcagcttt gatgcatttg atgattgtta tatcacgatt gacgattttg cgtatctcca
    21121 atcattttca gcgcaaaata gtttttatct gccaaattat attttagacg agctatttaa
    21181 gaatatctcc catcgaaaag tgattttatc tccagatgaa acaatgaaga ttggtggata
//
LOCUS       DACDXO010000224         7959 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E23605_2017
            SAMN12639989-rid9881463.guided.090, whole genome shotgun sequence.
ACCESSION   DACDXO010000224 DACDXO010000000
VERSION     DACDXO010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639989
            Sequence Read Archive: SRR10023389
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741914.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:42:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,972
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 33 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 17 of 73
            Pseudo Genes (multiple problems)  :: 10 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7959
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639989-rid9881463.guided.090"
                     /isolate="E23605_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            128..1564
                     /locus_tag="IWM35_002918"
     CDS             128..1564
                     /locus_tag="IWM35_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP9920674.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            1753..4200
                     /locus_tag="IWM35_002919"
     CDS             1753..4200
                     /locus_tag="IWM35_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HAP9920675.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            4430..5497
                     /gene="rlmN"
                     /locus_tag="IWM35_002920"
     CDS             4430..5497
                     /gene="rlmN"
                     /locus_tag="IWM35_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HAP9920676.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(5690..6793)
                     /locus_tag="IWM35_002921"
     CDS             complement(5690..6793)
                     /locus_tag="IWM35_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAP9920677.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            7346..7894
                     /gene="aac(6')"
                     /locus_tag="IWM35_002922"
     CDS             7346..7894
                     /gene="aac(6')"
                     /locus_tag="IWM35_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HAP9920678.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT         2665 a         1359 c         1728 g         2207 t
ORIGIN      
        1 tttcttacta ctcgaaacta agcgactttt tcacaacctc ctttacataa ttttattttg
       61 ttgtggaagg ggtttcgtgt tattctaaaa gaggaacgat tgaaggttat aggaaaggaa
      121 gagatgaatg gaacacaaag aacttacctc atttccaaaa gattttctgt ggggctctgc
      181 ttctgcagcg tatcaagtag aaggagcttg gcaagaagat gggaaaggag aatcagtctg
      241 ggatcgattt gtacggatac ctggaaagac atttaaagga acgaacggtg atttggcagt
      301 agaccactat catcgttata aagaagatat tgctttaatg aaagaacaag gtctgaaagc
      361 ttaccgcttc tctgtagctt ggacacggat ttttcctaat ggacgaggag aagtcaatca
      421 agctgggctc gctttttatg agagattgat cgacgaattg attgaaaacg atattgaacc
      481 tatgttgact ttatatcatt gggatcttcc acaagcattg caagatgaat ataatggttg
      541 ggaatcacgt cagatcattg aagatttcac tcattatgca gaaacattat ttgaggcatt
      601 ccgcgggaaa gtccattact ggattagttt gaatgaacaa aatatcttca cttcattagg
      661 ttatttgtta gcagcacatc cgccaggtgt gacagacccg aaacgaatgt atgaagttaa
      721 ccatattgct aatttagcaa atgcatctgt catcaataaa tttcacgagt tagaaatgcc
      781 tggaaaaatc ggaccaagct ttgcgtatac gccaaattat ccaattgaca gtaatcctga
      841 aaatgttctg gctgctgaaa atgcagaaga cctgatggcc aattattgga tggatgtcta
      901 tatgtgggga aaatatccga ttgctgccat gaggttctta gaagaaaaag gatgggcacc
      961 aacgattgaa gctggcgatg cagaattgct agaatctgct aaaccagatt ttcttgggat
     1021 caattactat caaacggcga cgaatgcatt caatccatta aacggagttg gtgctggaaa
     1081 aatgaatacg acaggaaaaa aaggttcatc agaagaaact ggagttcctg gaatgtataa
     1141 aaaagttgag aatccatttg tcgaacgaac aaattgggac tgggagattg acccagaagg
     1201 attaaggatc ggactgcgtc gtatcacaag ccgttaccgc atgccggtac tgattacgga
     1261 aaatggctta ggagagtatg acaagttgac agaagataaa caaatccatg acgactatcg
     1321 aatcaattac ttacaaagcc acgtcaaagc catcaaagaa gcaatttcgg atggagcaga
     1381 agtcctaggc tattgcacct ggtcttatac agatttgctt agctggctga acggttatca
     1441 aaagcgatat ggatttgtat atgttgatca agacgaaaca caagaaggat ctttggaacg
     1501 atataagaaa gacagttatt actggtacca aaaactgatt gaagaaaatg gagagaacct
     1561 ttagttcaca tacttaaaac aagaaggtac ggctggatat ggccgtgcct tttcttgata
     1621 aaatcattta gaaattaaga aaaactgtaa aataagctga aaaatgaaag aaagcgtcag
     1681 ttctttcaaa caaaacttta gttcccttac ttttttttgt ataatgaaac ttgatgaaat
     1741 gagggataat aaatggcaca aaagaaacgg cctactaaaa aaaggaaaaa gacgaaaaaa
     1801 acgaaaaagc aaaaacagca gcaggaacaa atgctgacga ttggtgttgg gtttgccttg
     1861 attttatttg ctgtcttcgg ctttttaaaa ttaggttttt taggaatatt gattgcaaac
     1921 ggctttcgga tcattgcagg aaatacttac caaatcctct gtcttttatt agccgtatta
     1981 ggattttgga ttgtcattaa gaatacagag ttttcaattg gtaaaaaccg acgctggttt
     2041 ggcggcatac tcttttattt cggtatctta ttattgttac atgcccattt atttggcaaa
     2101 ctccatactg gggaaccaaa tatcatgggg acgacatggg atttgttagc cagtgatata
     2161 aaacaaagtc aagtagacaa taacgttggt ggtgggatga ttggagcaat cctttatcat
     2221 tttacttact tcttgattgc acagcctgga agttatttag cagcagtgct attactggca
     2281 ggaggagcat tcctgttcag taatctggaa ggctatcagc tgttaaatgg tatccaatca
     2341 attggagaac gagtacagga attattggaa ggtgatcctg ccaagcaagc tcgaaagcaa
     2401 gcggcaaagg aagagagaat gaaacagcgc gcagaagcga aagaagcaag gcgcttagct
     2461 gcaaaagaag ctgcagaaaa agaagctgca gaatatgaga agaaaaaagc ggtgagccag
     2521 caaagggaaa atactgctga tgaatggcag gaaccagaaa atacagagcc ggagcagcta
     2581 agttttgttc caatcgacag ttttcaagaa aatatccatc ctgcgaatct agagaaacca
     2641 gtaccagata caccaaaaca gaccaataca gcagaaggtt tttcagatga gttgccagag
     2701 gatgacggta cctcattaga atttgagatt gaagcagaac aggaaaatca agactatgaa
     2761 ttaccgacag ttgatttgct tgattctata ccaactgtgg atcaaagtga tgagtataaa
     2821 aaaattgaaa aaaatatcgg cgtattagaa caaactttcc aaagtttcgg tgtcgatgcc
     2881 aaggtcgtca aagccagctt aggaccatcc gtcacaaaat tcgaagtcca gccggctgta
     2941 ggggtaaaag taagtaaaat tgtgaactta acagatgata tcgcgcttgc tttagcggcg
     3001 aaagatgtcc ggatggaagc accgattcca ggtaaatcac tgattggtat tgaagtacca
     3061 aatggaaaga tcagtatggt ttcctttaga gaaatcatcg aagcacaacc aaaccaccca
     3121 gataaattac tagaagtacc tttaggacgt gatgtttctg gacgggtaca aacggctgat
     3181 ctttcgaaga tgcctcactt actcgtggct ggttcgactg gtagtgggaa gtcagtagca
     3241 attaatggga ttatcacaag tatattaatg cgtgctaagc cccatgaagt aaaactgatg
     3301 atgatcgatc cgaaaatggt tgaattgaat atgtacaacg gcatacctca tctgttgacg
     3361 ccggtcgtga cgaaccctag aaaagcagcg caagccttac aaaaagtagt acaagaaatg
     3421 gaagaacgat atgaaaaatt cgcggcaacc ggagtaagaa atatttctgg ttacaatgaa
     3481 tttgttcaac agaaaaactt ggaaaacgga acgaaacatc caaccttacc attcatcgta
     3541 gtgatcgtag atgaattagc tgatttgatg atggtagcca gcaatgaagt tgaagatgcg
     3601 attattcgtt tagcgcaaat ggctcgggca gcggggattc atatgatcct agctactcaa
     3661 cgtccaagtg tggatgttat cacaggaatc atcaaagcaa atgtaccttc cagaatggca
     3721 tttgcggtat caagcggtac agattctcgg acgatcattg attctaatgg ggcagaaaag
     3781 ctcttagggc gaggagatat gctattcttg ccaatgggtg agaataaacc gatccgtgtg
     3841 caaggagcat tcatttctga ccacgaagta gaacgggtcg ttcaatttgt tacagatcag
     3901 caagaagctc attatgaaga gaaaatgatg ccgacagatg aagttgaaac gacaggtgct
     3961 ccagaacagc cacaagatga gctgtttgaa gaagcaaaag cacttgtcgt tgaaatgcaa
     4021 acggcaagta tttcccttct ccaaagaaga tttcggatcg gttacaaccg agcagcacgt
     4081 ttagtagatg aactggaagc tcatggagtt gttggcccgt cagaaggaag caagcctcgg
     4141 aaagtattca ttgagcagga agaaacatca gaaacgttgc cggacgataa tgacatgtaa
     4201 aacttgtgtg atgaagggtt gaaaaacgaa taaccaataa aaagacaagg catgatgata
     4261 ccaaaagaaa aaatggtttt tttccatctt ttctaatacg gtatgatgtc ttgtcttttt
     4321 agtttttgtg agattaaagt tctattcgat ttatttgtga agtattgcat taaggaaatg
     4381 aatcatgtat agtatgtagc gaaactttga gaaaaagagg gaaaatactt tggaaaaaca
     4441 gtccatttat ggcttgacga atgaagaatt aatcaattgg tttatagaaa acggcgagaa
     4501 aaaattccga gctgcccaag tttgggaatg gttgtatcaa aaacgtgtaa gtaatttcac
     4561 agaaatgacg aatctatcga aacaattgat cgaaaaatta tcggagcatt ttatcatcaa
     4621 tcctctaaaa cagatggtcg tccaagaagc ttcggatggt acagtaaaat atttgtttga
     4681 actccctgat aaaaacatga ttgagactgt actgatgaga caagaatatg gcttgtcagt
     4741 ttgtgtgacg acacaagtag gatgtaatat cggctgtacg ttttgtgcaa gcgggctgct
     4801 caaaaagaac cgtgatctga ctgctggaga aattgttgca cagatcatga tggtccaaca
     4861 ttattttgat gagcgcaatc ttggcgaacg cgtttcacat gtcgttgtaa tggggatcgg
     4921 tgagccattt gataattacg acaatgtaat ggactttttg catatcatca atgatgccaa
     4981 aggattggca attggtgctc ggcacattac agtttccact agtggattag cacacaaaat
     5041 caaggaattc gctaataacg gcttgcaagt caatctggct atttccttgc acgcgccgaa
     5101 taatgaggtg cgaacatcga tcatgcggat caaccgaagt ttcccaattg agaaattgat
     5161 ggaagctgtg gatgagtatt tagagaaaac aaaccgtcga atcacgtttg aatatatcat
     5221 gttgaatcaa gtaaatgata gaccagagca tgcgcaacaa ttagcagatc tattaaaaga
     5281 taagaaaaaa ttagcatatg tcaacttgat tccatataat ccagtcagtg aacatgatca
     5341 atattctcgc agtccaaaag ctgatgtatt gagattttat gatgtattga agaaaaacgg
     5401 cgtaaactgt gtgatacgta aagaacatgg tacagatatt gatgcagctt gcggacaatt
     5461 gcgaagcaag caaatgaaaa aaacagttgc caaataatcg tatcctactc gttttttcaa
     5521 ttaccataaa aggagcagtt ttcttaatta aggaactgtt caacttaaat aagagaaaag
     5581 attctatagc gaaagctata aagctttttt tgacaagaaa aaccctcatc aagcaaagaa
     5641 atgtcctcaa tttgatgaca gcctgcttga tgggggtttt agcaattatt taactgattt
     5701 tcttatatcc ttgctggctt tcttgtgtag catgataagt ttctacgtca agaatggcct
     5761 gtttaaattt ttctcgggtg atttcataag gcacatgttt aatatcattt gttgccatgg
     5821 ctttatctaa gattgcttct aaatcggtcg tgacattgag attcatgtcg gttaacgata
     5881 ctggaagttg gttctttttc ataaacacat agattttatc aaacaaatcg aactgctgat
     5941 ccatcatcaa taaggtcagt acaccataac tgacagatgc tccgtgaagg tatccctcca
     6001 ttttttctag aacagtcgta ccgtaataga atgagtgtgc catagcagaa ttatagttat
     6061 tgttaactaa aacagaaacc aatccggtag taacaatgat atccgaaacg acttgttcaa
     6121 ttgcatatga gggacgatcg tcggcactat ctttcaatgc ttgcgtacca taagtcaaga
     6181 gacgatcaat gcacattttt ccgatttgga ttcctaatct atccgtgtag cctagatcat
     6241 cgccacgagc agaaaaagta gattcgactt ctttggataa agcatcgcca atacctgccc
     6301 ataagtattt atcaggtgct tcggcaataa ttgctgtatc aatgaaggtg tgtacaggtg
     6361 gttttgaagg aaagaagagt ccgtccatta cgccttcttc gttatagatg acacataccg
     6421 cagtgacggg cgaacagttc gaaccaatag tgggaaaggt gaagactggt ttagctagtt
     6481 tttcaccaat gacttttaca gtatcgcaag cacggccacc accgactgca aacaggacgt
     6541 ctgctttttg gacactttca tgctggatca actgttcaat atttccataa gttgaattac
     6601 ccccgtacca gagactggcg acaacctcta tttcctgtgt caaagctact tttattttgt
     6661 tttctgcctt tgaaagtgca gtcttccctc caatcagcac tacttttgtc ccatattctt
     6721 tgacgattcc atttgctttt tcatacgcat ctgttccaat ggtataattt ggtaagaaaa
     6781 cactttgact catattcatt tccttctttc ttcgttgaaa cgggttaaaa ttttaaagag
     6841 tgatcgatct gactgcggct gtagatggag tagaggtgaa cctaagacag gataagtgat
     6901 gaataaggtg gcatgaacac cttgaaatca tcaataagta tcctaaaaac gaacaagtac
     6961 aaaaacattg aaaacgacaa taatgaattt ttgttcctct tagtatttct accgattccc
     7021 tttgcttttt tgtaattatc taaaaattct aaaaattaag attattatat gaaaacaggt
     7081 tttatttgtc aattcataat tgtttttttt taaagcaaaa gataatctat atcatgatga
     7141 acaaacagag ttttttcctc caattgatgg ctttcctgat ctttcctatt ttgaataaag
     7201 tgtttctgta taattttttt attttataag taagataaat aatgaaagaa tagagatact
     7261 gattggtagc taaagatttt tgagcgagaa catttcgttt tttcaaaagg actgttagaa
     7321 taagcaagaa caagggggaa gacgtatgat aatcagtgaa tttgaccgta ataatccagt
     7381 attgaaagat cagctttctg atttactgag actgacttgg ccggaagaat atggagacag
     7441 ctcggcagaa gaagtagaag aaatgatgaa tccagaacga atcgcggtag cagcggtaga
     7501 ccaagatgag ttagtaggat ttattggtgc aatccctcaa tacggtatca caggttggga
     7561 attgcatcca ttagttgtag aaagctcccg acgaaagaac caaataggta ctcgattagt
     7621 caattactta gaaaaagaag tagcttccag aggaggaatc acgatttatt taggtacgga
     7681 tgatttagac catggaacaa cgttaagtca aacggacctg tatgagcata catttgataa
     7741 agtggcttcc atccagaacc ttcgtgaaca tccgtatgaa ttctatgaaa aattaggtta
     7801 taaaatcgta ggtgtcttac caaatgcaaa tggctgggac aaaccggata tttggatggc
     7861 aaaaacgatt attcctcgac caaattctca ataataaaaa gaggttgtga cagaagtaat
     7921 cagcttcaag aaataagaag gaatttacga aaattactt
//
LOCUS       DACDXO010000225         6779 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E23605_2017
            SAMN12639989-rid9881463.guided.094, whole genome shotgun sequence.
ACCESSION   DACDXO010000225 DACDXO010000000
VERSION     DACDXO010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639989
            Sequence Read Archive: SRR10023389
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741914.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:42:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,972
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 33 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 17 of 73
            Pseudo Genes (multiple problems)  :: 10 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6779
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639989-rid9881463.guided.094"
                     /isolate="E23605_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            298..1566
                     /locus_tag="IWM35_002923"
     CDS             298..1566
                     /locus_tag="IWM35_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosaminidase"
                     /protein_id="HAP9920679.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            2053..2517
                     /locus_tag="IWM35_002924"
     CDS             2053..2517
                     /locus_tag="IWM35_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP9920680.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            2501..3130
                     /locus_tag="IWM35_002925"
     CDS             2501..3130
                     /locus_tag="IWM35_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAP9920681.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            3484..4962
                     /gene="msr"
                     /locus_tag="IWM35_002926"
     CDS             3484..4962
                     /gene="msr"
                     /locus_tag="IWM35_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Msr family ABC-F type ribosomal protection
                     protein"
                     /protein_id="HAP9920682.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            5009..5455
                     /locus_tag="IWM35_002927"
     CDS             5009..5455
                     /locus_tag="IWM35_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HAP9920683.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(5654..6169)
                     /locus_tag="IWM35_002928"
     CDS             complement(5654..6169)
                     /locus_tag="IWM35_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAP9920684.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            6286..>6779
                     /locus_tag="IWM35_002929"
     CDS             6286..>6779
                     /locus_tag="IWM35_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP9920685.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVG"
BASE COUNT         2215 a         1171 c         1384 g         2009 t
ORIGIN      
        1 gatagaaacg ccagaccgga tgtctgtcag caacatgatt atccgtccga cgacacagga
       61 agtataatcc ataacggata atatccagag agaattactt gttaaatagt tattcaaacg
      121 accatcaatt taaggcgtga atggagaaga atattcttca tttacgtctt tttatgcaga
      181 aaaagcaaat ttattaaaaa gaaacaaatg ttatttaaat atggacattg acttgctttt
      241 acttttttag ttacactgtt atttaggaaa agtttcataa ttcaaaggag aaaaaagatg
      301 aaagcaaaga atttattatt tagcgttatt cttttaggaa atctagcagt tgccaacaca
      361 acagtgctag ctgaagaatc gacagaaact gtcgcaactc agacatcgag ttcttcagaa
      421 acaggaattg aaaacgatac agaaagtact ttgcagtcag ctactgtctc agaaaataca
      481 atttcaaccg aagaaccaac aactgaaaca acagaacaaa cacaaaccga agacagtaca
      541 tcagcagaag ctcagaacaa tccagagaat gcagaagaaa tcacgaacca attgacagat
      601 gggcaaacat tgacacatac ccatcaagca gatttgtctg aattcgaaaa ctatactggc
      661 tatgcttttt atgctcgctc atctgctaat tctcaacaag cgttcattga tagtattgcc
      721 tcaactgcac aaagtttggc aagtgccaat gacttatatg catcagtcat gattgctcaa
      781 gccatcgttg aaagtggctg ggggaacagt actttggctt ctgcccctaa ttacaatcta
      841 tttgggatta aaggaagcta taatggccaa tctgttacga tgcctacttc ggaatatgtg
      901 aatgggcaat ggattacggt gaatgccgct tttagaaaat atccttcgta caaagaatca
      961 ttgcaagata acgtaacggt attgaagacg acttcttttc agccaggcgt ttactactat
     1021 tccggtgcat ggaaaagcaa tacgaactct tacaaagatg ctacggcttg gctaacaggt
     1081 cgttatgcca ctgcgcctaa ttatggttct acattgaaca atgtaatcga aacctataat
     1141 ctgacacagt atgatacagc accaacttca acttctccga tgtatcgtct gtataaccgt
     1201 catacaggag aacatttata tacattgaat gccggagaaa aaaattatct gccaactgta
     1261 ggctgggaat atgaagggat tgcttggcaa gcaccaaaca gcggacaacc tgtctaccgt
     1321 ttatacaatc cttacagtgg agaccatcat tacacaatgg cacaatctga gatcaactct
     1381 ttaacaaaaa tcggttggcg ttatgaaggt ctaagttttt attctggtgg ttccaagcca
     1441 atttatcgac tatttaatcc aaatgaaaaa actggtaccc atcactatac cttgagtgct
     1501 aaggaaagag attggctaac accgatgggc tggagatatg aaggaatagg attttatggt
     1561 tattaaaaag aggtagtgaa aaagctgccc tgcatcaagt agtaagaaca gccaaacaaa
     1621 aacagatttt catgtttttt gtttggttga acttattacc gcagatgcaa gtttttgaac
     1681 accgtttact caaaaaaggt tgtgacaaca tcttgtcata acttttttat tactgtttaa
     1741 gcatactgat ccaatttcta atattcgacg ggattcttgc aaaaggcgat ttcggaaatt
     1801 tttggcggtg taaaattatc tactgcagaa gagctatctt ctatagtttg ataagaatca
     1861 ataatcttgg ttttagcgtt gtgctttctt tatcattttt tccgttcatt ctacaaaaaa
     1921 tttcttttct ctatactttt ctattgagag cgtttttttt attacacagc tgattctttg
     1981 acttttttct ttttttattc tattattgac aagtcaatcg ttgacgagtc aattattaga
     2041 aaggaggaaa ttatgtcttt agaagagatt agtcaccttt tatatcaaat caaattagcc
     2101 gatcaaaaaa tcgtccagat ctttgaagaa aatatcggta taagtcttac tagatacgag
     2161 atcatgatga gactgaaaga agaaaatcca ttgctacaaa gcagtttgca agaaaagttg
     2221 cagattgatc aaggagcagt tactcgtcat ctgaaggtat tggaagcaaa tggctatgtt
     2281 tcgcgtcaga gaaatcctca gaacaaccgg gaagtgtatg tagcgctaac agaaaaagcc
     2341 cagaagaaaa tcaagcattg tgaaatggac cataaagata caggttccgt gctatccccg
     2401 gctttcagcg atgtagaaat gaaacaattt ctaacacttt tagataaatt gaatacccaa
     2461 ctttgcaata taacaacaga aacgaaagag gttaataata atggaacaaa aaaatgattt
     2521 ttcaacaatt atgactggta gaagatccgt tcgcgtatat gatgaaacat acaaaatccc
     2581 acacgaagaa atgctggaaa tgatcaaaga agcgacgact gcaccttctt ctgtcaatat
     2641 gaaaccatgg cgctttgtgg tcgctgaaag cgaggaagca aaagaaatct tgcgtccttt
     2701 gatccgtttc aatacgagac aaaatgatac ttcttctgca atggtcatga tttttggcga
     2761 tatgcaatgt tacgaatatg gcgaagaaat ctacaaccaa gcttacgaat cagggaagat
     2821 gccaaaagaa gtcagagatc aacaattagc tgcgatcatt ccttattaca agagtttttc
     2881 aagagaagaa atgaacgatg tagtcaaagt cgattctagc ttagcggcta tgcaattcat
     2941 gttagttgcg cgtgatcatg gctatgaaac aaatccaatt ggtggattcg aagcagatca
     3001 gttggcagag gcatttggtt tggataaaga ccgttatgtt ccagttatta ttttatcaat
     3061 aggaaaagcg gtagaagaag gttacgagtc tgttcgttta gcaccagaaa agatcactat
     3121 gttcgaataa aaggttttca gttactattc agtagctatt gctttttttg aatcgtgtgt
     3181 ggtaacctaa taaaaaagga ggtcggaaaa atgactgcat cgatgaaatt acgtttcgaa
     3241 cttttgaata acaactaaat aaattcaaaa gacatgtata aacaacaatt tcatggatgg
     3301 agtatgtcca ttttttcgaa cgacagtaga ataggtgaaa gctatgacag atttggtgat
     3361 ttagtcatag ttatttcgtt cagagtagga aaagatgtga tgcagttttt ttgcaaacag
     3421 atttttctgc tcttttttag tatcgataat tgttgtttta catgaaatag gagtgtaaaa
     3481 catatggaaa atttagcagt aaatataaca aatctacaag tcagctttgg caaccagcta
     3541 gaactatcta ttgattctct tcgtgtctat cagcaagatc gtatagggat cataggagaa
     3601 aatggtgtcg gtaaatcgac tttgctcaaa ctaatagccg gtgaactttt tcccgatcat
     3661 gggaaaatcc aaacagagat caccttcaac tacctgcctc aattaaccta tctcgctgaa
     3721 gcaaaggacc taaatttgga attagccagt catttccagt taagactgga agaaacttca
     3781 gagcggaaat ggagcggagg ggaagaacga aagatcgagt tgatacgtct tctttcttct
     3841 tatgaacaag ggatgcttct agacgagcca acaacccatc tagatagaaa aagtattgat
     3901 cgactgattg aagagcttcg ttattattat ggtacgctgg tttttgttag tcatgatcgc
     3961 tattttctag atgagctagc atcgaaaatc tgggaagtaa aagacggaga aatccgagag
     4021 ttttcgggga attatagtgc ctatctcact caaaaggaat tggagaaaaa gactcagcta
     4081 cgagaagcag agtcgatcat gaaagagaaa aaacgattgg aaaaatcgat ccaagaaaag
     4141 aaaaaacaag cggaaaagtt ggaaaaagtg tccagtaaaa agaaaaagca acaaatcaga
     4201 ctggatcggt tgtcttcctc taaacaaaaa gacagtgtac aaaaagccat ccaaaagaat
     4261 gcgaaaacat tagagagaag actccaaaaa ataggagaaa caaccaagcc gcagcagatg
     4321 aaacaaatcc gttttccagt accaaaatct cttgaactcc acagccgcta tcccatcatg
     4381 ggacaaaatg tccaattgga aagaagtggg agaacattac tggtaaatgg tgattttcag
     4441 ttttctttag gtaaaaaaat cgcgattgtc ggtgaaaatg gttcaggtaa gacaacctta
     4501 ttagaacata tccgcaaaca aggagaagga atccttctct ctccgaaagt aagctttcaa
     4561 gtatatcagc aaaagggtta tcaaatgaca tctgaagaat ccatcattcg ttttgtcatg
     4621 agacaaacag agttttcaga atcgcttgtc cgtagtttgc tgaatcactt agggtttgct
     4681 caggaaactt tgacgaaacc gttatgtaca ttaagtgggg gagaagcgac ccgtctgacg
     4741 attgctttgc tttttactaa gccaagtaat gtgttgctgt tagatgaacc gactaatttt
     4801 attgatatgg caacgatcga agctttagag aagctaatgc aaatatatcc gggaacgatt
     4861 ttgtttactt ctcatgattc ttactttgtc gagcgtacgg ctgatgaagt ttatgaaata
     4921 aaagggcaga aaataaaaaa agtacttacg agaaattttt aaatgtgcta cgatgatttt
     4981 cgtgacaaat ttaaaggagt ggtacagatt gcctacaaat aaaaaagaag gaataatttt
     5041 tacaacaatg atgtgttttt tgatggtctt ggggatgagc gcgtataatt tatggctgca
     5101 tcaagatctt catttatcaa gtttgttgat tggtctagtg cctggattcg tggtggcttt
     5161 cattttggat gtcttcatca tcggtgtgct ggcgaaaaaa attgccttta aactaccagt
     5221 aaatccagaa agcaaggtcc aaatgatctt aactatttca tgcttgatgg ttttaggaat
     5281 ggtgacatgt atgtctttat tcggtgtgat catggaacaa ggaatacctg atcaattatt
     5341 ctctgcttat atgaatgctt ggaaaatgaa tgtgattgcc gctttaccgt tgcagttgat
     5401 catcgttggc ccgctttcaa gaagtgtgct aaaagcaatt caaagtaatg aatgatggaa
     5461 cactgttatt tgaaaaaagg ccttggcatg ctttttgtca tagtctcttg aatgcagttc
     5521 tagaaaaata gtgatccgaa cgataggttt tggcctgtcg ttcggatcgc tttttctttt
     5581 ccaactgtat tgctctttta ttgaagataa tttgctcttc aatagatatt tctagaaagg
     5641 ttctgtttat cccttactca aaaaactgct gcatttgttc tcttacttgc tgacaccatt
     5701 ggagttcctg ctgacaaagc ataattctgc gctggatcac aacataacgt ccaaattctt
     5761 gtttcttgtc tagcgagagg tcattcaatt ttttttgatt tttttgcatg attttgttca
     5821 gctgttgttc tctagtgaat agcagtccag tagctgttgt tttatcgagc gtcgaaaaag
     5881 catagatttt tgctagaaat tcatcttttt gcgtaggagc gggagtttcc tctttgatcc
     5941 attcctctac aagagcacgt ccttcatcag tcaagtggta gatctttttt tgagagtgct
     6001 cgtctttgtc caaaacggtc acataaccct gctggtctaa cttggcaagc gttgtataga
     6061 tctgactgtg atgggcttcc caaaaaattg tgatcagctg cttcaattca tacccagaac
     6121 gtggtttgga taaaagcacg cataataata tataggctaa agtattcata aaaatcctac
     6181 ctctcgtctt attttcttta ttatatcaca gtaaagtttc cacgttttac atgtgcatat
     6241 tgacatgtaa aataaatcat gtatgatgct aatagagaga aagggatgga aatattggag
     6301 aatcaaatag atatacaaga acaaacaaat gaagaaacaa aggggaaaag ttcagcgcta
     6361 gttgtagcag ctacctgtat gatcgctttt atgggagtgg gtctagtaga cccaatctta
     6421 aaaacgattg ctgcacagtt aaacgctacg ccagctgaaa cgaccctgtt atttactagt
     6481 tacatgttag tcactggtgt ggtgatgcta tttacaggtt tcttatctag tcggatcggt
     6541 ttgaaaaaaa cattgatgac aggattgttt atcatcgttg tattcgctgg tcttggcgga
     6601 ttatctggga atatcggaat gctgattggg ttacgtgccg gctggggtgt cgggaatgct
     6661 ttatttgtct ctactgcttt agcaacgatc gtcagtattt tagttggtaa cacagaaaaa
     6721 gcaatcatga tgtatgaagc agcacttggc tgcggaatgg ctgttggccc tctagtcgg
//
LOCUS       DACDXO010000226         6425 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E23605_2017
            SAMN12639989-rid9881463.guided.097, whole genome shotgun sequence.
ACCESSION   DACDXO010000226 DACDXO010000000
VERSION     DACDXO010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639989
            Sequence Read Archive: SRR10023389
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741914.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:42:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,972
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 33 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 17 of 73
            Pseudo Genes (multiple problems)  :: 10 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6425
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639989-rid9881463.guided.097"
                     /isolate="E23605_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            296..571
                     /locus_tag="IWM35_002930"
     CDS             296..571
                     /locus_tag="IWM35_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9920686.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            584..2656
                     /locus_tag="IWM35_002931"
     CDS             584..2656
                     /locus_tag="IWM35_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HAP9920687.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKRVQ"
     gene            2821..4707
                     /locus_tag="IWM35_002932"
     CDS             2821..4707
                     /locus_tag="IWM35_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAP9920688.1"
                     /translation="MLKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKT
                     SLLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVR
                     AYEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIS
                     TLSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDR
                     YFLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMR
                     AGVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRIND
                     QTLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQ
                     ENEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEK
                     RRLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVA
                     DKLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKE
                     WETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSE
                     WVED"
     gene            4719..5666
                     /locus_tag="IWM35_002933"
     CDS             4719..5666
                     /locus_tag="IWM35_002933"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HAP9920689.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            5686..6213
                     /locus_tag="IWM35_002934"
     CDS             5686..6213
                     /locus_tag="IWM35_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAP9920690.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(6276..>6425)
                     /locus_tag="IWM35_002935"
     CDS             complement(6276..>6425)
                     /locus_tag="IWM35_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HAP9920691.1"
                     /translation="VILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFS
                     VLFFT"
BASE COUNT         2140 a         1063 c         1389 g         1833 t
ORIGIN      
        1 tcaattctga ttcatcagtc ccataaatta tagagccgat ttttgtcgca gccccatttt
       61 tttgttgtga taaaagaaca agaaaggcag gaatgggaaa gtaaacatcc ttttgtcaga
      121 aacttttatc tttcttaagt tttacttgat atctgatact atttggaata gaatgaggcg
      181 ttgaaaaatt tatggaaatt tctaactagc aataaacgat gaaacaatga aatatttttc
      241 atccaaaaag aactttgcta taatgatgga agtttgtgta taggagatct ggaaaatgag
      301 agagagtttt gaacaacaaa aaaaactgtt gcatgatcgc tatggcgccc tttcgatgga
      361 tgaccgccgt caaatacttt gtaagttgcg taaaagaaat atattgatgt atcgtcagtt
      421 ggaacgtctg aaacatgatc tgcttcgttt ggagtcaaaa cgtgtacagt gtgaattaga
      481 ggggaaccaa acacaggttg aagtagtcga aaccaaaatt ttaaagaaaa aagaacaatt
      541 tttaaaaatg cttacacaaa ataaaaaata aaggggatgg acgatggaat ttgctgagtt
      601 agttattgta tttgcagtaa cgatcacctt atcgaacata gctagccgca ttttgccaat
      661 gattccagct ccactgatcc aaattttcct tggagtggta ttaggtctga cagagtgggg
      721 acaatcaatc gattttgaac ctgaattgtt cttagttatg attattgcac cattgttatt
      781 tagagaagga gaaaaagcag atatttcttc gattctcaaa aatttcggga caattttgtt
      841 cttggcattt ggaggagtca ttttgaccct tgtcggggta ggtgcgacgt tatcattcct
      901 gttgccaagt gtcccacttg ctgcctgctt tgcttttgga gcagcgttag gtccgacaga
      961 tgcggtagca gtcagttctc tttctggacg agtgaatatc cctaaaaagg cgatgcatat
     1021 tttggaagga gaaggtcttt tgaatgatgc atctggtgtt actgccttcc agtttgctct
     1081 tggtgcgctg atcaccggat ctttttcagc agtaaacgcg gggatgagtc tagtagtttc
     1141 tagtattggg ggagctttga tcggcttttt gcttgtctgg ttcaaacaaa aaataattca
     1201 tttgatcgaa aaagcatcag cacaagatgt cacagggtat ctgctgatcg aacttttgct
     1261 tccttttcta gcctatgtat tggcagaggc atttgatgta tctggaatca ttgctgcagt
     1321 ggcagcaggt attctccaag cttctggttt taggaaaatc tctgtgtttg atgcagaatt
     1381 gtcgagcttg tcccacagta catggacaac tattgctttt acactgaatg cattagtatt
     1441 tatattttta ggaatcgaat tgacgcaagt gttctcgcca gtatggggag acggtttata
     1501 tccaaacggt cttttattag cgattatcgt attaatctct gtcatgcttt ttgtgatcag
     1561 attcatttct atcagtcttt tttatgtatt caaagatggc agtaaaaagt tcaaaaaaca
     1621 gttgaacgag atcttgatct taacatttgg cggtgtaaag ggaacagtca gtttggctac
     1681 gatttttatt ttgccatttt caatcaataa tatgatgttt taccagcggt ctttgctttt
     1741 gtttctgacg gcgggtgtca tcttagtgac tttggtcata gggatcattg tcttgcctat
     1801 gctgacggaa acagaggaag cgaaaagcac ggatttgaat gctttgatga ttttagaaga
     1861 ggtcgttgaa gttttgcgaa aagaaataaa ggaaattgat cgtaatacaa aagaattttt
     1921 agccactgaa gcagtgatag aaaattatca agaacggata cgtgatctgt acttggaaga
     1981 tttaaccgat gatgaaaagc aagaagtaca agaaatacaa gcactgattt tgtcgatcga
     2041 acgggatgga ttggatgaaa gttatcgttc gggcaagctt tcttctaatg gttatcgttt
     2101 ttactcccgt tttctttctc gattcgaaca ttctatcaca agccagattt tgtcttttat
     2161 cggcttttgg ttcattgttg tccgtaggtt aatgcgaatc gttctccatc cgaaaatgtt
     2221 ttggcaaaga agatatgctg atcgtcagac atttattagt gatgaagata tccaagaaat
     2281 cagagagaca tatcaaaaaa atactcagtt gattattgaa agtttagata acttgactga
     2341 tatttatgat gaaacgatgg ttcaattttt tatccagcaa cgcaaacttg aaggaataaa
     2401 aatgaactcg gggaatttga tttcttcgtg gatgatccag caagatagtc tgtttacgaa
     2461 aaaaatgcta agaggttatt atcttgaaag aaaaatcatt gatgaatacg aagttgctga
     2521 aaaaatcacg acgttttctg cgaacagcta tcgcaggaat atcaatttgt tagaatctta
     2581 tacgatgaac aaaccatctg ataacttttc tttccgattt gcgtttcgag caaagtcaaa
     2641 aaaacgagtc caataaaata aaaaggtaaa aattttcaag ttttataaaa aacaaaacat
     2701 atcagaacag gatcgaatcg gtcctgttct ttttttgaga acacaaaaag aacaaatacc
     2761 aaaaaaattt tcctgaagtt cactattttt atgatacaat atccaagata aggagggaac
     2821 atgttgaaag aattaaaagt aactgacctc aaaaaaactt atggtgaaaa agatttgttt
     2881 gaccagattt cttttttggt ccacgaaaaa gaccgtatcg ggttgattgg gacgaacggg
     2941 acgggtaaaa caagtctctt aaatattatt gccggtatcg atagcagcga cggtgatcgg
     3001 caaacagtgt tttatccaac tgattaccgt attggttatc tttctcaagc agctgaattt
     3061 tccgatgaat taactgttct ccaagcagtc tttcaaggaa acagcccctt gatccaaact
     3121 gttcgagcat atgaggaagc attgatcgaa ctaggggaaa acggagaaga ccctgatgtg
     3181 caaaaacgtt atgctaaagc agaagaacaa atgaataaag aagatgcttg gactacagat
     3241 accaacgcaa agattatttt gcaaaaatta ggaatcagtg aattagataa aaaaatcagt
     3301 actttatcag gtggtcagaa aaaacgagtt agtctggcgc aagtgttgat tgatgaaccc
     3361 gatttactat tactagacga accaacaaac catttagatt atgaagcgat cgagtggttg
     3421 gaaaactatt tgaaacaata ccgtggcgca ctattgatgg ttacccacga tcgttatttt
     3481 ctagatagag tagcaaaccg aatcttcgaa ctttcatttg gaaaattata cgagtataaa
     3541 ggaaattatg aagcttacgt cttagaaaaa gcagaacgtg atcgtgtaga agtagaacaa
     3601 gaagaaaagc gcaagcggct ttataaacag gaattagctt ggatgagggc tggcgtacag
     3661 gcacgtggca ccaagcagca agcacggatc aaccgtttcg aagatctaaa ggagaacttg
     3721 taccaagtaa accaagaaga tgatttagag ctgaacctag cgacacaacg tttagggaaa
     3781 aaagtactgg aaatcaaaga tggcagttac cgtataaatg accaaacact gctagaacat
     3841 ttagatttat tgattcaatc tcgggaaaga ctaggtatta ctggtaaaaa tggtgcggga
     3901 aaatcgacat tgcttaatat tctagctggc agaattcccc tggatagcgg tacaatgtct
     3961 atcggtgaaa cggttcatct tgcgtattat acacaagaaa atgaagaaat ggcaccagat
     4021 aaacggatga ttgcttactt acaagaagct gctgaagaag caaaaacagc agatggcagt
     4081 caaatcggag tagcagaatt acttgaacga tttttatttc caagatttat gcatgggaca
     4141 ctgataggca aactttcagg cggagaaaaa cgccggttat ttttgttgaa actgctgatt
     4201 cagcaaccta atgttttatt attggatgaa ccgaccaatg atttggacat agcaacattg
     4261 acgattttgg aagattattt tagaagtttt ccaggtgctg tgatcactgt ttcccatgac
     4321 cgttatttct tggacaaggt agcagataag ttattagtat tccaaggaaa cggaaaacaa
     4381 gaactttatt acggaaacat gtccagttat ttactgaagc aaaaagagac ccagcagcct
     4441 gctgaaaaag cgaaaccaaa aatacagagc aaggaacctg ctgaaaagaa gaaattatct
     4501 tacatggaac aaaaagaatg ggaaacgatt gaagatgaga ttgcagaact tgaagaaaaa
     4561 atctcattgc tccaagaaga gatgaatcat caaggagata atttcactcg attacaagaa
     4621 ttgcaaaacg acgtatctga aacagaagca caacttgaag aaaaaatggc acggtgggaa
     4681 tacttgagtg aatgggtaga agattaggag gaaaaaggat ggaacaagcg tatcttgact
     4741 taggaagaaa attgttggaa gaagggcatc gtaaagaaga cagaacaggc acaggcacat
     4801 taagcttatt cggataccaa atgcggtttg atctgcaaaa agggtttcct ttgttaacaa
     4861 cgaaacgagt accttttgga ttgatcaaaa gcgaattgct ttggttttta aaaggagata
     4921 cgaatatccg ctatctatta caaaataaaa accatatttg ggatgaatgg gcatttgaac
     4981 gctacatcaa aagtccagac tataatggac cagatatgac tgattttggc catagatgtt
     5041 tgacagatcc tgaattcaat gaagaatata aaaagcagag caagcttttt tgcgagaaga
     5101 tcctaaccga tgattccttt gctgaaacat acggcgatct tggacacatc tacggctatc
     5161 aatggcgtca ctgggaaaca aaagacggtg gctttatcga tcaaatcaaa caagtgatcg
     5221 aagcaatcaa aaagactcct gattctagaa gattgatcgt ttctgcatgg aatccggaag
     5281 atgttcctag catggctttg cctccttgtc atacgatgtt tcaattttat gttcaagagg
     5341 gacggctaag ctgccaattg tatcaacgca gtggggatgt gtttctaggc gttccgttca
     5401 acattgcaag ttatgcgttg ctgactcacc taattgcaca tgaaacaggt ttagcggttg
     5461 gcgaatttgt tcacacatta ggagatgctc atctttatat caatcatatc gaacaaatga
     5521 aagaacaatt gagccgagaa atacgcgcat ttccgacttt agttttgaat caggaaaagc
     5581 agtcagtatt tgattttgag atggaagata tccaattaga aggatatgac ccacatccgg
     5641 cgatcaaagc tcctatcgct gtttagaatc agaaaggatg agaaaatgtt agctgcaatt
     5701 tgggcacagg atgaaaacgg attgattgga aaagaagacc gtcttccttg gcgtttgcca
     5761 aatgatctga aattcttcaa acaaatgaca gaagcaaata cactagtgat gggacgaaaa
     5821 acattcgaag ggatgggtag ccgtccgttg ccgaatcgtc agacgattgt tttgacgaga
     5881 gattcttcct ataaagcaga aggcgtgcat gtcatgcata gtgtggaaga cgtgcttgct
     5941 ctggaaaaag aaacagacgg tatctttttc atcgctggag ggtcagctgt ttatcaagaa
     6001 tttctgccat tttgtacgat attataccgt acagtcatcc atcatgtatt cgatggagat
     6061 gcttattttc cacctgttga ctggtctgat tggtcactga tcaatactag tcaaggagaa
     6121 atggatgaaa aaaatcgtta tccgcatcaa tttgaaacgt atcagcgtaa tgagaatcct
     6181 gtaaaaagcg ggacaggcga attgacgata taaaaaagct atggatagac tagctgttat
     6241 ttagagctgt cccatccata gctttatttt ttttattaag taaagaataa aacagagaag
     6301 tacataaaac ctgcacctaa cataacaaac aaatgccaaa caacatgcat atagcggata
     6361 gaattcaagc tataaaagaa tgcgcctatc gtgtaagaaa gcccgcctgc taccatcaaa
     6421 ataac
//
LOCUS       DACDXO010000227         5986 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E23605_2017
            SAMN12639989-rid9881463.guided.103, whole genome shotgun sequence.
ACCESSION   DACDXO010000227 DACDXO010000000
VERSION     DACDXO010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639989
            Sequence Read Archive: SRR10023389
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5986)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741914.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:42:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,972
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 33 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 17 of 73
            Pseudo Genes (multiple problems)  :: 10 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5986
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639989-rid9881463.guided.103"
                     /isolate="E23605_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(41..1027)
                     /locus_tag="IWM35_002936"
                     /pseudo
     CDS             complement(41..1027)
                     /locus_tag="IWM35_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006701269.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS1542 family transposase"
     gene            1179..1874
                     /gene="vanR"
                     /locus_tag="IWM35_002937"
     CDS             1179..1874
                     /gene="vanR"
                     /locus_tag="IWM35_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HAP9920692.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            1852..3006
                     /locus_tag="IWM35_002938"
     CDS             1852..3006
                     /locus_tag="IWM35_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HAP9920693.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
     gene            3221..4189
                     /locus_tag="IWM35_002939"
     CDS             3221..4189
                     /locus_tag="IWM35_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HAP9920694.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            4182..5213
                     /gene="vanA"
                     /locus_tag="IWM35_002940"
     CDS             4182..5213
                     /gene="vanA"
                     /locus_tag="IWM35_002940"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HAP9920695.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALVVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            5219..5827
                     /locus_tag="IWM35_002941"
     CDS             5219..5827
                     /locus_tag="IWM35_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HAP9920696.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPKRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
BASE COUNT         1811 a         1161 c         1348 g         1666 t
ORIGIN      
        1 gggattttat tctttattta aaactttgca acagaaccct atttccgata accgtgtatt
       61 gaaaggcatc ttcgaagcca ttatccaata tttcgcaggc ttttgtatat ttaggctggt
      121 cgatatattc accgactaag gcattcttag ctgttcgagc gagttcaata tccgtaaact
      181 taaagatcgc ttttactgct tctctaaacg gttttgaatt cttttttgga atggaactga
      241 agatgtttct taaaaaatgg acctggcatc tctgccaact tgcgttggta aatgacttac
      301 gaatcgcaga cactaggcct ttatgggcat cagaaatgat gagttctgtc ccctttaggc
      361 cgcgttcttt taagtattca aagaagatgg accatgtgtc atcactttct tcattttgaa
      421 tcatgaagcc aatgatttca cggtcgccac cttctgttat cccgatcgca atatggcagc
      481 ttttagaaag cactcgatgg tcctcacgga cttttatgta gagaacatca gtcatcagat
      541 aaggataatt cgtacctgag agtgaacggt tctgccattc gttgaccatc gggtctaact
      601 gctcagtcag gctagaaaca aaagatttcg atacagattt tccacatagc tcttcaacaa
      661 tctttgaaac tttacgtgtc gaaacgcccg aaacatacat ctcaagcatt gaagcgagca
      721 gtgccttttc atttcgctga taacgctcaa acaccgtcgg tgaaaattca ccatcacgtg
      781 ttctaggcac ttttaattcg agtgtaccca cacgagtcgt aaagtctcgc tcataatagc
      841 catttctttg actctgacga ctttctgaac gttcatagtc atcggcttga atatattctg
      901 ttcgttgatt ttccatcaat tggttgaaaa cagtggttaa aatattttta gaaacatcat
      961 cttttaccga atgttcaata atactttgaa cctcttcgtt gttcagtgta aaatgtactt
     1021 gggtcatgta acgtcctcct gggtatgttt ttgtggttaa aaacattgta ccgtaaaagg
     1081 gctgttacat ggccttttat cttttacaca attatatgga ctttatccta taatctgata
     1141 aattattaag ggcaaactta tgtgaaaggg tgataactat gagcgataaa atacttattg
     1201 tggatgatga acatgaaatt gccgatttgg ttgaattata cttaaaaaac gagaattata
     1261 cggttttcaa atactatacc gccaaagaag cattggaatg tatagacaag tctgagattg
     1321 accttgccat attggacatc atgcttcccg gcacaagcgg ccttactatc tgtcaaaaaa
     1381 taagggacaa gcacacctat ccgattatca tgctgaccgg gaaagataca gaggtagata
     1441 aaattacagg gttaacaatc ggcgcggatg attatataac gaagcccttt cgcccactgg
     1501 agttaattgc tcgggtaaag gcccagttgc gccgatacaa aaaattcagt ggagtaaagg
     1561 agcagaacga aaatgttatc gtccactccg gccttgtcat taatgttaac acccatgagt
     1621 gttatctgaa cgagaagcag ttatccctta ctcccaccga gttttcaata ctgcgaatcc
     1681 tctgtgaaaa caaggggaat gtggttagct ccgagctgct atttcatgag atatggggcg
     1741 acgaatattt cagcaagagc aacaacacca tcaccgtgca tatccggcat ttgcgcgaaa
     1801 aaatgaacga caccattgat aatccgaaat atataaaaac ggtatggggg gttggttata
     1861 aaattgaaaa ataaaaaaaa cgactattcc aaactagaac gaaaacttta catgtatatc
     1921 gttgcaattg ttgtggtagc aattgtattc gtgttgtata ttcgttcaat gatccgaggg
     1981 aaacttgggg attggatctt aagtattttg gaaaacaaat atgacttaaa tcacctggac
     2041 gcgatgaaat tatatcaata ttccatacgg aacaatatag atatctttat ttatgtggcg
     2101 attgtcatta gtattcttat tctatgtcgc gtcatgcttt caaaattcgc aaaatacttt
     2161 gacgagataa ataccggcat tgatgtactt attcagaacg aagataaaca aattgagctt
     2221 tctgcggaaa tggatgttat ggaacaaaag ctcaacacat taaaacggac tctggaaaag
     2281 cgagagcagg atgcaaagct ggccgaacaa agaaaaaatg acgttgttat gtacttggcg
     2341 cacgatatta aaacgcccct tacatccatt atcggttatt tgagcctgct tgacgaggct
     2401 ccagacatgc cggtagatca aaaggcaaag tatgtgcata tcacgttgga caaagcgtat
     2461 cgactcgaac agctaatcga cgagtttttt gagattacac ggtataacct acaaacgata
     2521 acgctaacaa aaacgcacat agacctatac tatatgctgg tgcagatgac cgatgaattt
     2581 tatcctcagc tttccgcaca tggaaaacag gcggttattc acgcccccga ggatctgacc
     2641 gtgtccggcg accctgataa actcgcgaga gtctttaaca acattttgaa aaacgccgct
     2701 gcatacagtg aggataacag catcattgac attaccgcgg gcctctccgg ggatgtggtg
     2761 tcaatcgaat tcaagaacac tggaagcatc ccaaaagata agctagctgc catatttgaa
     2821 aagttctata ggctggacaa tgctcgttct tccgatacgg gtggcgcggg acttggattg
     2881 gcgattgcaa aagaaattat tgttcagcat ggagggcaga tttacgcgga aagcaatgat
     2941 aactatacga cgtttagggt agagcttcca gcgatgccag acttggttga taaaaggagg
     3001 tcctaagaga tgtatataat tttttaggaa aatctcaagg ttatctttac tttttcttag
     3061 gaaattaaca atttaatatt aagaaacggc tcgttcttac acggtagact taataccgta
     3121 agaacgagcc gttttcgttc ttcagagaaa gatttgacaa gattaccatt ggcatccccg
     3181 ttttatttgg tgcctttcac agaaagggtt ggtcttaatt atgaataaca tcggcattac
     3241 tgtttatgga tgtgagcagg atgaggcaga tgcattccat gctctttcgc ctcgctttgg
     3301 cgttatggca acgataatta acgccaacgt gtcggaatcc aacgccaaat ccgcgccttt
     3361 caatcaatgt atcagtgtgg gacataaatc agagatttcc gcctctattc ttcttgcgct
     3421 gaagagagcc ggtgtgaaat atatttctac ccgaagcatc ggctgcaatc atatagatac
     3481 aactgctgct aagagaatgg gcatcactgt cgacaatgtg gcgtactcgc cggatagcgt
     3541 tgccgattat actatgatgc taattcttat ggcagtacgc aacgtaaaat cgattgtgcg
     3601 ctctgtggaa aaacatgatt tcaggttgga cagcgaccgt ggcaaggtac tcagcgacat
     3661 gacagttggt gtggtgggaa cgggccagat aggcaaagcg gttattgagc ggctgcgagg
     3721 atttggatgt aaagtgttgg cttatagtcg cagccgaagt atagaggtaa actatgtacc
     3781 gtttgatgag ttgctgcaaa atagcgatat cgttacgctt catgtgccgc tcaatacgga
     3841 tacgcactat attatcagcc acgaacaaat acagagaatg aagcaaggag catttcttat
     3901 caatactggg cgcggtccac ttgtagatac ctatgagttg gttaaagcat tagaaaacgg
     3961 gaaactgggc ggtgccgcat tggatgtatt ggaaggagag gaagagtttt tctactctga
     4021 ttgcacccaa aaaccaattg ataatcaatt tttacttaaa cttcaaagaa tgcctaacgt
     4081 gataatcaca ccgcatacgg cctattatac cgagcaagcg ttgcgtgata ccgttgaaaa
     4141 aaccattaaa aactgtttgg attttgaaag gagacaggag catgaataga ataaaagttg
     4201 caatactgtt tgggggttgc tcagaggagc atgacgtatc ggtaaaatct gcaatagaga
     4261 tagccgctaa cattaataaa gaaaaatacg agccgttata cattggaatt acgaaatctg
     4321 gtgtatggaa aatgtgcgaa aaaccttgcg cggaatggga aaacgacaat tgctattcag
     4381 ctgtactctc gccggataaa aaaatgcacg gattacttgt taaaaagaac catgaatatg
     4441 aaatcaacca tgttgatgta gcattttcag ctttgcatgg caagtcaggt gaagatggat
     4501 ccatacaagg tctgtttgaa ttgtccggta tcccttttgt aggctgcgat attcaaagct
     4561 cagcaatttg tatggacaaa tcgttgacat acatcgttgc gaaaaatgct gggatagcta
     4621 ctcccgcctt ttgggttatt aataaagatg ataggccggt ggcagctacg tttacctatc
     4681 ctgtttttgt taagccggcg cgttcaggct catccttcgg tgtgaaaaaa gtcaatagcg
     4741 cggacgaatt ggactacgca attgaatcgg caagacaata tgacagcaaa atcttaattg
     4801 agcaggctgt ttcgggctgt gaggtcggtt gtgcggtatt gggaaacagt gccgcgttag
     4861 ttgttggcga ggtggaccaa atcaggctgc agtacggaat ctttcgtatt catcaggaag
     4921 tcgagccgga aaaaggctct gaaaacgcag ttataaccgt tcccgcagac ctttcagcag
     4981 aggagcgagg acggatacag gaaacggcaa aaaaaatata taaagcgctc ggctgtagag
     5041 gtctagcccg tgtggatatg tttttacaag ataacggccg cattgtactg aacgaagtca
     5101 atactctgcc cggtttcacg tcatacagtc gttatccccg tatgatggcc gctgcaggta
     5161 ttgcacttcc cgaactgatt gaccgcttga tcgtattagc gttaaagggg tgataagcat
     5221 ggaaatagga tttacttttt tagatgaaat agtacacggt gttcgttggg acgctaaata
     5281 tgccacttgg gataatttca ccggaaaacc ggttgacggt tatgaagtaa atcgcattgt
     5341 agggacatac gagttggctg aatcgctttt gaaggcaaaa gaactggctg ctacccaagg
     5401 gtacggattg cttctatggg acggttaccg tcctaagcgt gctgtaaact gttttatgca
     5461 atgggctgca cagccggaaa ataacctgac aaaggaaagt tattatccca atattgaccg
     5521 aactgagatg atttcaaaag gatacgtggc ttcaaaatca agccatagcc gcggcagtgc
     5581 cattgatctt acgctttatc gattagacac gggtgagctt gtaccaatgg ggagccgatt
     5641 tgattttatg gatgaacgct ctcatcatgc ggcaaatgga atatcatgca atgaagcgca
     5701 aaatcgcaga cgtttgcgct ccatcatgga aaacagtggg tttgaagcat atagcctcga
     5761 atggtggcac tatgtattaa gagacgaacc ataccccaat agctattttg atttccccgt
     5821 taaataaact tttaaccgtt gcacggacaa actatataag ctaactcttt cggcaggaaa
     5881 cccgacgtat gtaactggtt cttagggaat ttatatatag tagatagtat tgaagatgta
     5941 aggcagaggg ttctgttgca aagttttaaa taaagaataa aatccc
//
LOCUS       DACDXO010000228         5197 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E23605_2017
            SAMN12639989-rid9881463.guided.110, whole genome shotgun sequence.
ACCESSION   DACDXO010000228 DACDXO010000000
VERSION     DACDXO010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639989
            Sequence Read Archive: SRR10023389
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5197)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5197)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741914.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:42:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,972
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 33 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 17 of 73
            Pseudo Genes (multiple problems)  :: 10 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5197
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639989-rid9881463.guided.110"
                     /isolate="E23605_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            102..1223
                     /locus_tag="IWM35_002942"
     CDS             102..1223
                     /locus_tag="IWM35_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAP9920697.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            1481..2983
                     /locus_tag="IWM35_002943"
     CDS             1481..2983
                     /locus_tag="IWM35_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HAP9920698.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            3050..3577
                     /locus_tag="IWM35_002944"
     CDS             3050..3577
                     /locus_tag="IWM35_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAP9920699.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            3788..4849
                     /locus_tag="IWM35_002945"
     CDS             3788..4849
                     /locus_tag="IWM35_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAP9920700.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            4852..>5197
                     /gene="murQ"
                     /locus_tag="IWM35_002946"
                     /gene_synonym="yfeU"
     CDS             4852..>5197
                     /gene="murQ"
                     /locus_tag="IWM35_002946"
                     /gene_synonym="yfeU"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="catalyzes the cleavage of the lactyl ether moiety
                     of N-acetylmuramic acid-6-phosphate (MurNAc-6-P) to form
                     N-acetylglucosamine-6-phosphate (GlcNAc-6-P) and lactate;
                     involved in MurNAc dissimilation pathway; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HAP9920701.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEG"
BASE COUNT         1786 a          878 c         1160 g         1373 t
ORIGIN      
        1 tttttatttt aaatggctaa gttcattata aaatccgcca ttgttttgtt tgaaaaaaat
       61 gaaaaaaagt gtcttactta agaagaaagt gagggataca gatggatatt ttggcggcaa
      121 ttgattcatt taaaggaagt gcttcttcat ttgaactaaa tcaggcagtt ctaaatggct
      181 gggaagatcg taaaggaaga aaaatcaatg tgccgattgc agatggcgga gaaaatactg
      241 cagaagcaat ctattacgcg ctcaggggaa catggcgtac cattgatggg atggatctct
      301 tattccgtca gcgaaaaatc agctatttat tgacaacttg gcaaggaaaa aacaccgcag
      361 tcatcgaatc tgcacaagca ttggggttag atctacttga taaaccaacg gatcaaacta
      421 tccgacttgc ttccagttat gccttaggag aacttattaa agatgcactg cttcaagaag
      481 tagaacaaat tattgtcact ttaggaggaa gcgggtgtag cgatggtggt ctaggattac
      541 tccaatcctt gggtgcttct ttagaagggt acacagaagg aaatccttta ttatctacta
      601 aaaaacttca ttttgatcga agaaacgttc ttgtttctaa agtaaaattg attatcgctg
      661 cagatgttac caatccatat acaggtcaac aaggagctgc ttggcaattt ggaagacaaa
      721 aaggaggaat agatacgacg ctagctttct tagataaaca agcaggaaag attgctcagc
      781 aaataaaaga tttctgggag gtggatttag atgatattcc aggaagtgga gcagcaggcg
      841 gtattggtgg agcccttttt ttgttgggag cgaagatggt ttctggtttc gatttagtca
      901 gccaaatcac tggattggaa aaaaagatcg aacaagcaga tgtcatcatt acaggagaag
      961 ggcggatcga tcagcaaacg atccacggaa aagttccata cggtgtagca gtgcttgcaa
     1021 aaaaatatcg aaaaccaata atcgctattt gcggaagtag agaccaagag ctagaaggac
     1081 tttctgattt tctcccaatt gtcttaagta ttcatttggg tccaataggt ttagaagaag
     1141 caatggaaca caggcaaaca ttagaaaaaa caaaaatctt aggacagaca cttagcagat
     1201 ttttcaccat cctcccgaag tagttgacaa aatcattttc tttatgctat cgtaatttca
     1261 aatataggac aaagtgaggg atccgtgcat tgaaaaatta agtcgattga tgaaagctgc
     1321 attcagactg taccttgtgg agacaaggaa gcagtgtgca gattttcggg attggcttgg
     1381 ttttttcgat gtatgggtct ttttgtgtgt atccaaccct tgcatcctct ttagccagtt
     1441 ctgaaaatag gactggtctt tttttacgag gtgatggaaa atgtctaaaa tcgaaataaa
     1501 aaatctgaca ttcggctacg acagccaagg cacattatta tttgaacaag caaatctaaa
     1561 ttttgacaca caatggaaac taggacttat cggacgaaac ggtcgaggaa agacaacttt
     1621 actgaatatt ctacaaaaca aactacctta tcaagggcaa gtaatccatc agcaagaatt
     1681 tgcctatttc ccgcaacaga caaaagataa agaacgttta acctattacg tgttaaatga
     1741 tattacggat tttgagatat gggaaatcga aagagagctc caattgatgc aaacagatcc
     1801 tgaaatctta tggagagaat tcagcacact atcgggggga gagaagacaa aagtcctact
     1861 ggcactttta tttgtggatg acactcattt cccgttaatc gatgaaccaa cgaatcattt
     1921 ggatatctct ggtagaaaac aagtagcggc ttatttgaaa aagaaaaaac aaggcttcat
     1981 cgtggtcagc catgaccggg gatttatcga tgaagtagtg gaccatgttt tagcaatcga
     2041 aaaaagtcaa ctggaacttt atcaagggaa tttctctatc tatgaagaac agaaaaaact
     2101 tcgtgatgaa tttgaaatgg ctcaaaatga aaaattgaaa aaagaagtca gtaggctaaa
     2161 gaaaacagca gctgaaaaag ccgaatggtc tcgttcccga gaaggagata aaacaaagaa
     2221 acaagtcgga ttcatcgata ctgaatctag acgagtgaat aaaggagcag tgggtgctga
     2281 tgctgcacgg acgatgaaac gatccaaagc aatcgtgaat cggatggaga cccagatcag
     2341 cgagaaagaa aaactattaa aagatatcga atatatcgat tctttgacga tgaatagcca
     2401 agcgtctcac cataagcgac ttttaagcgt agaagatctt caattagggt atgaaaatct
     2461 gttattcgag ccaattcatt ttacaatcga gcctcatcag cgggtggcga tttcaggtcc
     2521 taacggtgca ggaaagtcat ccattatcca ttatcttctg ggggcattca acggcaaggt
     2581 tataggagaa aaataccagc caaaacatct gagcattagt tatgcaagcc aaaattatga
     2641 agacaatcga ggaacgttgg cggaatttgc agagaaaaac caagtagact accaagcatt
     2701 tttgaacaac ctccgaaagc ttgggatgga aagagatgtt tttcataaca agatcgagca
     2761 gatgagtatg ggccaacgga aaaaagtgga attggctaaa tctttatcac agccagctga
     2821 actatataca tgggatgaac cattgaatta tttggatgtc ttcaatcaag aacaattaga
     2881 acaactgatc ttgaacgtga aacctgccat gttactagtg gaacatgatc aaaccttcct
     2941 ggataaagta tctactgaga ttatttctct tgagagaatc taacccttgt aatctcaaga
     3001 acaacaaggt atggttaagg ggaactttgc caacgaagga gagaatcaca tgctttatgt
     3061 aacgagacac ggagaaacaa cttggaacgc acaaggatta gtttgcggac gggcagatgt
     3121 gccgctgact gaaaaaggac agatgcaggc acagaaacta gcagaaaagg tagtagattt
     3181 gcctgttcca atcacgaaaa tcatccattc ccctcttcaa cgagcaagag atacagcaca
     3241 agcagttgct gatcgtctca gcttgccgct gaccgttgat gaacgattgg tagaaatgga
     3301 ttttggggat tatgatggac tgccaagcaa agatgagaac tttcaaaaag cacgtttagc
     3361 gttcgctgta cgattcccaa atggggagtc agtcttagat gtgtatgcac ggatcgttcc
     3421 tttattgaaa gaatgcatcg aagacgaaga aaatgtctat ctgttagttt gccataatgc
     3481 cttgataaga gtgatcaatg cttattttca tccgatgcct aatgaggggt tctttacatt
     3541 catggtagat aatacagaat tggtttctta tgagtaatta tgtttttgtg ttatttaaat
     3601 aaaatatttc aaggcactgc ttttgcttag cagtgccttt tttaggttat gacaattctt
     3661 ttgataatat aaactcaacc tttagaaatg aaatttaatt attttaataa aagtattgaa
     3721 attttgtttc tttagatata tactctagtc atagaaatga agacggatac agaaaaagag
     3781 ggataatatg tttggctttt caatatttat gaacgattct ttgtcagaag agaagaaaca
     3841 gtatatccgc caaatggcat ccaatggctt tgtcggtatt ttcacttcca tgcatatacc
     3901 agaagatgat gtttcggcat atcaaaagcg attgatcgaa ctaggaagcg aagcgaaaaa
     3961 gaataaacta gaattgatgg tcgatatttc aggagaggca ttggaacgag cgggtttttc
     4021 attagaagat ctaacgcctt tgaaagcaat tggtgtaaca ggtttaagaa tggattatca
     4081 tatttccaat cagcagattg ccgactggtc acatcaattg aaaatcagtt tgaatgctag
     4141 cacgatcacg cctaaagata tcgatgaatt gaaagaagca gaagctgatt tctcacagat
     4201 tgaagcttgg cataattatt atccacgtcc cgaaacaggg ctggacaaag aatggtatca
     4261 gaaaaaaaat caatggctca aaattcaagg tctccttgtc caaggttttg taccaggaga
     4321 tacagagttg agaggcccac tatatcaagg attgccaact ttggaagaac atcgagggat
     4381 ccatcctctt gcaggcgctc tagatctatt agcaagtaac acggatattg tttacatcgg
     4441 agatgcaggt ctgtcagaaa acgttcagga gcaatttgct tctttccaaa aagaacagac
     4501 agtcttgctc catacggaac ctgtagatga agaattttac gaatatatat tagggaagca
     4561 tacgaatcgg caagacgatg caagagatgt catacgaagc gtagatgcac gttttcgaga
     4621 gattccgcct atcccagcaa gaaatacagc tacgcgtatg aaagggtcta ttactttaga
     4681 taacgaaaag tatttgcgct atatgggtga gatccaatta accaaatatg atttgccagc
     4741 ggatgaaaaa gtgaatgtag tagctaaagt tatcaaagaa gagctaccat tgatcaacca
     4801 aatcaaagct ggtatgaact atcaatttat aagaaaagaa ggaagataaa aatgagcaaa
     4861 atcaatttag agaacctaac aacggaacgc agaaaccaag aaacttttgg attagatgag
     4921 atgagtattt ctcaagccgt tcaactaatg aacaaagaag atcagaaagt ggccgctgcg
     4981 gttgaaaaag aactgacaag tatcgaacca gtgattgcta acacgattga gtcctttaaa
     5041 aaaggtggac gtctgattta tatgggagcc ggaacaagtg gaagactagg tgtgttagat
     5101 gctgctgaat gcgtaccgac attcggtgta gaaccggaaa tggtcatagg tctaattgct
     5161 ggtggagaag aagcaatgac tgtagctgtc gaagggg
//
LOCUS       DACDXO010000229         3668 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E23605_2017
            SAMN12639989-rid9881463.guided.130, whole genome shotgun sequence.
ACCESSION   DACDXO010000229 DACDXO010000000
VERSION     DACDXO010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639989
            Sequence Read Archive: SRR10023389
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3668)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741914.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:42:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,972
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 33 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 17 of 73
            Pseudo Genes (multiple problems)  :: 10 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3668
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639989-rid9881463.guided.130"
                     /isolate="E23605_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..164)
                     /locus_tag="IWM35_002947"
     CDS             complement(<1..164)
                     /locus_tag="IWM35_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HAP9920702.1"
                     /translation="MKYILSFCTPKLNYLLAYIRLYSGVLHLRDSVRVSEKEKIKVTE
                     MYTSINGELC"
     gene            394..891
                     /gene="dfrG"
                     /locus_tag="IWM35_002948"
     CDS             394..891
                     /gene="dfrG"
                     /locus_tag="IWM35_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HAP9920703.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDNFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            complement(963..2915)
                     /locus_tag="IWM35_002949"
     CDS             complement(963..2915)
                     /locus_tag="IWM35_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HAP9920704.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(2922..3218)
                     /locus_tag="IWM35_002950"
     CDS             complement(2922..3218)
                     /locus_tag="IWM35_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9920705.1"
                     /translation="MQIGVLKWLQFTKQKGVHFPMQFLEPKNKNAKSVDWEISEQVRV
                     IVKQYAEYAERTESEAVDEFLLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            complement(3393..>3668)
                     /locus_tag="IWM35_002951"
     CDS             complement(3393..>3668)
                     /locus_tag="IWM35_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAP9920706.1"
                     /translation="TVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLFPLYHGS
                     AKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFKIEYTKKRQRLAY"
BASE COUNT         1132 a          636 c          591 g         1309 t
ORIGIN      
        1 ttacataatt caccatttat tgaagtatac atttctgtaa cttttatttt ttctttttct
       61 gatactctaa ccgaatctcg taaatgtagt actccactat aaaggcgtat atatgcaagt
      121 aagtagttca gttttggagt acaaaatgac aaaatgtact tcaaaatgga ctactttttt
      181 cattttaact tacataccaa attacataca aaatgattta taaaatgcaa ttgaaaatga
      241 taattgaatg aaaatttgtg attttgtggt attatgataa tataacttat agaaaaaatt
      301 gcttattcaa ctaacgggca ggataatgag aaaaggtttt ttgatagaaa taagaaatta
      361 gatggtaaat tagttaaaaa ggctgtgtta taaatgaaag tttctttgat tgctgcgatg
      421 gataagaata gagtgattgg caaagagaat gacattcctt ggaggattcc caaggactgg
      481 gaatatgtta aaaatactac aaagggacat ccgataatat taggtaggaa gaaccttgaa
      541 tcaatcggaa gagccttacc tgacagaaga aatattattc tgacgagaga taaggggttt
      601 acctttaatg gttgtgaaat tgttcattca atagaagatg tttttgagtt atgtaaaaac
      661 gaagaagaaa tttttatttt cggaggagaa cagatttata atttgttttt cccttatgtt
      721 gagaaaatgt acatcacaaa aatacatcat gaattcgaag gagataattt ttttccagaa
      781 gtgaattatg aggaatggaa tgaggtattt gcccaaaaag ggataaagaa tgataaaaat
      841 ccgtataact actattttca tgtatatgaa agaaaaaact tattgagtta acgggtgcgt
      901 taattgaaga agaacagtaa agataaagca caagagcttg ggattactcc aagctctttt
      961 tgtcatttga aataaattat atctttccaa tcaaattgtt tatctgtaat ccctaatctt
     1021 tgagcaggtg tcaaaacctt tttatccgct gatttaaaag ggcgacaaaa gttgtagtaa
     1081 gttcttagaa ttgtaatcgc aaattgagca tacttcggat tgaagttagc gtaaatatag
     1141 ctcttcttat caccacgagc tgttgttaat ggtctttcaa gagatgatat ataacgtctt
     1201 atctgttgaa taaagctatt ggtcgaatgg tcattaacat ttaatagcat ctttgcgatt
     1261 tcgtttggtt ccagagcaga taaatccgtt ctacaatcaa ctgaataaaa tcctttatcc
     1321 ttagtggcta atggatgttt gatagggtta tcggcatatt tgcgatatct ttctccatct
     1381 ttactaatag cctcttcgtg gaattgatga cttgttagta attcagttag ataatgagat
     1441 gccaaagtcc gtagaaactt tgttttgaat cctctgatat ctccccaatc gagcagttct
     1501 gcttttgctt gtccgaattc ttttaagcat tgttttctgc ttttcgtctt gttaacttgg
     1561 caaataaaat gatgtgcatc gctcaacctt aattctttcg tgaatactct ataaaaggcg
     1621 tttctaatag agaagtcatc atcactaatc attcgccatt cgtcggcatt aaccatttgt
     1681 ttaattaacc aatagtgagc agttgtagta taaggagcat caacatttaa accatcgaca
     1741 aatctactcc tattagtaat tttccctaat tcgtgaagat aagcattcct attttcgcta
     1801 tcgttagcgg atggttcttg tggataataa gaccaatcta acctatcatt tttacgacta
     1861 aaagtattga gatggtcttc tttgaatttt cttgtatctt catttaattc atccattgaa
     1921 atattccaat cataggcaac atcagaacgg aacacatatc ttgataagac ctcagcagat
     1981 acaactatat acgtttgcat attaaggtct tcaaaccccg tatatttctt tgaaccttgt
     2041 cctttcttac ggacgttatt taggtagtag tgcatcttgt ccgtattaag ccacatctcg
     2101 ttaaatttct ttgtttgaag gggttgtgtc tcatatcgtt ctaaaaactc caaacaacgc
     2161 ctatacagcc actccagctt atgatagtag gttccaactc caattcctaa aatatcacaa
     2221 gtacgactga ctgaaaccct tccaacaacc atcttagcga acattggaag aatggtgttc
     2281 ttttgttggt gataagtagt ggtttcttct ctttttggta gaacattagt gaacttctta
     2341 cacgccttac attgatagcg ttgagatttg cccctacttt tccccctttt gtaaaattgc
     2401 ttcggctcat tgaatggggt tgattcttct tccgagcagc cttctttatg aaattgataa
     2461 tctggctcaa catcttggat gctattgatt tctataaggc gtttaatttc ttcaacaaca
     2521 gaccaattag ataaaggggt tactgtacag ttttgcgata cacctctgcc aataggattg
     2581 tcattgcagt acataccttt atgaccttta tctttggaac taccagtcat tttatatcga
     2641 cttggtttcc cttttacatc tttgaatttg tgctgttctt tcccaaaatt acaacaaaac
     2701 ggattgatgc agtaattata ttgaatggga tattcagaac cgttccattt aaaagacgtt
     2761 ggcaagaaca acattttgtt ataccttgtt gcaaatcgtt ttggagttaa taaatcatat
     2821 tgtttagccc tatcttttaa ttcttcgttt gaaatcggaa ttttaacatc tacaatttca
     2881 tcttccttcg tggctaatct tttaagcttg gtcattgtct atcaacccac tctatctttg
     2941 atacccattt tttcaacaat ccgcttatta cttcgcttgt tggcaatcca ttcaataaac
     3001 ttcttatcgt ctaaaatgtt caataagaat tcatccactg cttcgctttc agtacgctct
     3061 gcatattcag cgtattgctt aactatcacc cttacttgct ctgatatttc ccaatccaca
     3121 gacttagcgt tcttattttt tggctcaaga aattgcatag gaaaatgcac ccctttttgc
     3181 ttagtaaatt gtaaccattt taacacacca atttgcattc ctacaataat tatttcacgt
     3241 tcgcattttt atatacattt taacttacat tttgtggtgt aaattaacaa caaacgttga
     3301 tttaacaata aaaaacgagt atcaaattca gttgcaattt gatactcatt taggtttaca
     3361 ttttcacttt ttgtactcca aaactgaact acttaatatg caagacgttg tctttttttt
     3421 gtatattcaa ttttgaaaac atttccgcaa agttcagacg gacctcgatg tgttgatgaa
     3481 taaaatttat tagtaataac ttctataagg ttatcaatcc ctatattact ttttgcactt
     3541 ccatgataaa gagggaacag agaacaattc tgaaatctta tgctttcctc ttgttcgagt
     3601 tccaatgctt ctaatgattt accggacata tatttctcta aaaggtcatc gtttccctct
     3661 attaccgt
//
LOCUS       DACDXO010000230         3668 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E23605_2017
            SAMN12639989-rid9881463.guided.131, whole genome shotgun sequence.
ACCESSION   DACDXO010000230 DACDXO010000000
VERSION     DACDXO010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639989
            Sequence Read Archive: SRR10023389
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3668)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741914.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:42:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,972
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 33 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 17 of 73
            Pseudo Genes (multiple problems)  :: 10 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3668
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639989-rid9881463.guided.131"
                     /isolate="E23605_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..164)
                     /locus_tag="IWM35_002952"
     CDS             complement(<1..164)
                     /locus_tag="IWM35_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HAP9920707.1"
                     /translation="MKYILSFCTPKLNYLLAYIRLYSGVLHLRDSVRVSEKEKIKVTE
                     MYTSINGELC"
     gene            394..891
                     /gene="dfrG"
                     /locus_tag="IWM35_002953"
     CDS             394..891
                     /gene="dfrG"
                     /locus_tag="IWM35_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HAP9920708.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            complement(963..2915)
                     /locus_tag="IWM35_002954"
     CDS             complement(963..2915)
                     /locus_tag="IWM35_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HAP9920709.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(2922..3218)
                     /locus_tag="IWM35_002955"
     CDS             complement(2922..3218)
                     /locus_tag="IWM35_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9920710.1"
                     /translation="MQIGVLKWLQFTKQKGVHFPMQFLEPKNKNAKSVDWEISEQVRV
                     IVKQYAEYAERTESEAVDEFLLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            complement(3393..>3668)
                     /locus_tag="IWM35_002956"
     CDS             complement(3393..>3668)
                     /locus_tag="IWM35_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAP9920711.1"
                     /translation="TVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLFPLYHGS
                     AKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFKIEYTKKRQRLAY"
BASE COUNT         1131 a          637 c          591 g         1309 t
ORIGIN      
        1 ttacataatt caccatttat tgaagtatac atttctgtaa cttttatttt ttctttttct
       61 gatactctaa ccgaatctcg taaatgtagt actccactat aaaggcgtat atatgcaagt
      121 aagtagttca gttttggagt acaaaatgac aaaatgtact tcaaaatgga ctactttttt
      181 cattttaact tacataccaa attacataca aaatgattta taaaatgcaa ttgaaaatga
      241 taattgaatg aaaatttgtg attttgtggt attatgataa tataacttat agaaaaaatt
      301 gcttattcaa ctaacgggca ggataatgag aaaaggtttt ttgatagaaa taagaaatta
      361 gatggtaaat tagttaaaaa ggctgtgtta taaatgaaag tttctttgat tgctgcgatg
      421 gataagaata gagtgattgg caaagagaat gacattcctt ggaggattcc caaggactgg
      481 gaatatgtta aaaatactac aaagggacat ccgataatat taggtaggaa gaaccttgaa
      541 tcaatcggaa gagccttacc tgacagaaga aatattattc tgacgagaga taaggggttt
      601 acctttaatg gttgtgaaat tgttcattca atagaagatg tttttgagtt atgtaaaaac
      661 gaagaagaaa tttttatttt cggaggagaa cagatttata atttgttttt cccttatgtt
      721 gagaaaatgt acatcacaaa aatacatcat gaattcgaag gagatacttt ttttccagaa
      781 gtgaattatg aggaatggaa tgaggtattt gcccaaaaag ggataaagaa tgataaaaat
      841 ccgtataact actattttca tgtatatgaa agaaaaaact tattgagtta acgggtgcgt
      901 taattgaaga agaacagtaa agataaagca caagagcttg ggattactcc aagctctttt
      961 tgtcatttga aataaattat atctttccaa tcaaattgtt tatctgtaat ccctaatctt
     1021 tgagcaggtg tcaaaacctt tttatccgct gatttaaaag ggcgacaaaa gttgtagtaa
     1081 gttcttagaa ttgtaatcgc aaattgagca tacttcggat tgaagttagc gtaaatatag
     1141 ctcttcttat caccacgagc tgttgttaat ggtctttcaa gagatgatat ataacgtctt
     1201 atctgttgaa taaagctatt ggtcgaatgg tcattaacat ttaatagcat ctttgcgatt
     1261 tcgtttggtt ccagagcaga taaatccgtt ctacaatcaa ctgaataaaa tcctttatcc
     1321 ttagtggcta atggatgttt gatagggtta tcggcatatt tgcgatatct ttctccatct
     1381 ttactaatag cctcttcgtg gaattgatga cttgttagta attcagttag ataatgagat
     1441 gccaaagtcc gtagaaactt tgttttgaat cctctgatat ctccccaatc gagcagttct
     1501 gcttttgctt gtccgaattc ttttaagcat tgttttctgc ttttcgtctt gttaacttgg
     1561 caaataaaat gatgtgcatc gctcaacctt aattctttcg tgaatactct ataaaaggcg
     1621 tttctaatag agaagtcatc atcactaatc attcgccatt cgtcggcatt aaccatttgt
     1681 ttaattaacc aatagtgagc agttgtagta taaggagcat caacatttaa accatcgaca
     1741 aatctactcc tattagtaat tttccctaat tcgtgaagat aagcattcct attttcgcta
     1801 tcgttagcgg atggttcttg tggataataa gaccaatcta acctatcatt tttacgacta
     1861 aaagtattga gatggtcttc tttgaatttt cttgtatctt catttaattc atccattgaa
     1921 atattccaat cataggcaac atcagaacgg aacacatatc ttgataagac ctcagcagat
     1981 acaactatat acgtttgcat attaaggtct tcaaaccccg tatatttctt tgaaccttgt
     2041 cctttcttac ggacgttatt taggtagtag tgcatcttgt ccgtattaag ccacatctcg
     2101 ttaaatttct ttgtttgaag gggttgtgtc tcatatcgtt ctaaaaactc caaacaacgc
     2161 ctatacagcc actccagctt atgatagtag gttccaactc caattcctaa aatatcacaa
     2221 gtacgactga ctgaaaccct tccaacaacc atcttagcga acattggaag aatggtgttc
     2281 ttttgttggt gataagtagt ggtttcttct ctttttggta gaacattagt gaacttctta
     2341 cacgccttac attgatagcg ttgagatttg cccctacttt tccccctttt gtaaaattgc
     2401 ttcggctcat tgaatggggt tgattcttct tccgagcagc cttctttatg aaattgataa
     2461 tctggctcaa catcttggat gctattgatt tctataaggc gtttaatttc ttcaacaaca
     2521 gaccaattag ataaaggggt tactgtacag ttttgcgata cacctctgcc aataggattg
     2581 tcattgcagt acataccttt atgaccttta tctttggaac taccagtcat tttatatcga
     2641 cttggtttcc cttttacatc tttgaatttg tgctgttctt tcccaaaatt acaacaaaac
     2701 ggattgatgc agtaattata ttgaatggga tattcagaac cgttccattt aaaagacgtt
     2761 ggcaagaaca acattttgtt ataccttgtt gcaaatcgtt ttggagttaa taaatcatat
     2821 tgtttagccc tatcttttaa ttcttcgttt gaaatcggaa ttttaacatc tacaatttca
     2881 tcttccttcg tggctaatct tttaagcttg gtcattgtct atcaacccac tctatctttg
     2941 atacccattt tttcaacaat ccgcttatta cttcgcttgt tggcaatcca ttcaataaac
     3001 ttcttatcgt ctaaaatgtt caataagaat tcatccactg cttcgctttc agtacgctct
     3061 gcatattcag cgtattgctt aactatcacc cttacttgct ctgatatttc ccaatccaca
     3121 gacttagcgt tcttattttt tggctcaaga aattgcatag gaaaatgcac ccctttttgc
     3181 ttagtaaatt gtaaccattt taacacacca atttgcattc ctacaataat tatttcacgt
     3241 tcgcattttt atatacattt taacttacat tttgtggtgt aaattaacaa caaacgttga
     3301 tttaacaata aaaaacgagt atcaaattca gttgcaattt gatactcatt taggtttaca
     3361 ttttcacttt ttgtactcca aaactgaact acttaatatg caagacgttg tctttttttt
     3421 gtatattcaa ttttgaaaac atttccgcaa agttcagacg gacctcgatg tgttgatgaa
     3481 taaaatttat tagtaataac ttctataagg ttatcaatcc ctatattact ttttgcactt
     3541 ccatgataaa gagggaacag agaacaattc tgaaatctta tgctttcctc ttgttcgagt
     3601 tccaatgctt ctaatgattt accggacata tatttctcta aaaggtcatc gtttccctct
     3661 attaccgt
//
LOCUS       DACDXO010000231         2371 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E23605_2017
            SAMN12639989-rid9881463.guided.148, whole genome shotgun sequence.
ACCESSION   DACDXO010000231 DACDXO010000000
VERSION     DACDXO010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639989
            Sequence Read Archive: SRR10023389
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741914.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:42:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,972
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 33 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 17 of 73
            Pseudo Genes (multiple problems)  :: 10 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2371
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639989-rid9881463.guided.148"
                     /isolate="E23605_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            22..1941
                     /gene="tet(M)"
                     /locus_tag="IWM35_002957"
     CDS             22..1941
                     /gene="tet(M)"
                     /locus_tag="IWM35_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019117172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HAP9920712.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYTSGKLLEALELEQEESIRFHNCSLFPVYH
                     GSAKNNIGIDNLIEVITNKFYSSTHRGQSELCGKVFKIEYSEKRQRLAYIRLYSGVLH
                     LRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRKKIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIEIKEPTVIYMERPLKKAEYTIHIEVPPNPFWASIGLSVA
                     QLPLGSGVQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            2060..2227
                     /locus_tag="IWM35_002958"
     CDS             2060..2227
                     /locus_tag="IWM35_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP9920713.1"
                     /translation="MCPVCGNKTRLKIREDTELKKFPLYCPKCRQENLIEIKQFKVTV
                     ITEPDAKTQSR"
     gene            complement(2287..>2371)
                     /locus_tag="IWM35_002959"
     CDS             complement(2287..>2371)
                     /locus_tag="IWM35_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAP9920714.1"
                     /translation="MDSFTDKELSLMESLASGINEARNIED"
BASE COUNT          818 a          361 c          482 g          710 t
ORIGIN      
        1 ttttgaatgg aggaaaatca catgaaaatt attaatattg gagttttagc tcatgttgat
       61 gcaggaaaaa ctaccttaac agaaagctta ttatataaca gtggagcgat tacagaatta
      121 ggaagcgtgg acaaaggtac aacgaggacg gataatacgc ttttagaacg tcagagagga
      181 attacaattc agacaggaat aacctctttt cagtgggaaa atacgaaggt gaacatcata
      241 gacacgccag gacatatgga tttcttagca gaagtatatc gttcattatc agttttagat
      301 ggggcaattc tactgatttc tgcaaaagat ggcgtacaag cacaaactcg tatattattt
      361 catgcactta ggaaaatggg gattcccaca atctttttta tcaataagat tgaccaaaat
      421 ggaattgatt tatcaacggt ttatcaggat attaaagaga aactttctgc cgaaattgta
      481 atcaaacaga aggtagaact gtatcctaat atgtgtgtga cgaactttac cgaatctgaa
      541 caatgggata cggtaataga gggaaacgat gaccttttag agaaatatac gtctgggaaa
      601 ttattggaag cattagaact cgaacaagag gaaagcataa gatttcataa ttgttccctg
      661 ttccctgttt atcacggaag tgcaaaaaac aatataggga ttgataacct tatagaagtg
      721 attacgaata aattttattc atcaacacat cgaggtcagt ctgaactttg cggaaaagtt
      781 ttcaaaattg agtattcgga aaaaagacag cgtcttgcat atatacgtct ttatagtggc
      841 gtactgcatt tgcgagattc ggttagaata tcggaaaagg aaaaaataaa aattacagaa
      901 atgtatactt caataaatgg tgaattatgt aaaatcgata aggcttattc cggggaaatt
      961 gttattttgc aaaatgagtt tttgaagtta aatagtgttc ttggagatac aaaactattg
     1021 ccacagagaa aaaagattga aaatccgcac cctctactac aaacaactgt tgaaccgagt
     1081 aaacctgaac agagagaaat gttgcttgat gcccttttgg aaatctcaga tagtgatccg
     1141 cttctacgat attacgtgga ttctacgaca catgaaatta tactttcttt cttagggaaa
     1201 gtacaaatgg aagtgattag tgcactgttg caagaaaagt atcatgtgga gatagaaata
     1261 aaagagccta cagtcattta tatggaaaga ccgttaaaaa aagcagagta taccattcac
     1321 atcgaagttc caccgaatcc tttctgggct tccattggtc tatctgtagc acagcttcca
     1381 ttagggagcg gagtacagta tgagagctcg gtttctcttg gatacttaaa tcaatcgttt
     1441 caaaatgcag ttatggaggg gatacgctat ggctgtgaac aaggattgta tggttggaat
     1501 gtgacggact gtaaaatctg ttttaagtat ggcttatact atagccctgt tagtacccca
     1561 gcagattttc ggatgcttgc tcctattgta ttggaacaag tcttaaaaaa agctggaaca
     1621 gaattgttag agccatatct tagttttaaa atttatgcgc cacaggaata tctttcacga
     1681 gcatacaacg atgctcctaa atattgtgcg aacatcgtag acactcaatt gaaaaataat
     1741 gaggtcattc ttagtggaga aatccctgct cggtgtattc aagaatatcg tagtgattta
     1801 actttcttta caaatggacg tagtgtttgt ttaacagagt taaaagggta ccatgttact
     1861 accggtgaac ctgtttgcca gccccgtcgt ccaaatagtc ggatagataa agtacgatat
     1921 atgttcaata aaataactta gtgtatttta tgttgttata taaatatggt ttcttgttaa
     1981 ataagatgaa atatttttta ataaagattt gaattaaagt gtaaaggagg agatagttat
     2041 tataaactac aagtggatat tgtgtcctgt atgtggaaat aaaacacgat taaagataag
     2101 ggaagatact gaattaaaaa aattccccct ctattgtccg aaatgcagac aagaaaattt
     2161 aattgaaata aagcagttca aagtaactgt gattacagag ccagacgcaa agacgcagag
     2221 ccgataaaat gagattaata caatctcatt ttatcggctc tttccgttat gtatggattc
     2281 ttttaattag tcttcgatgt ttcttgcttc gttgataccg ctggctaaag attccattaa
     2341 ggatagttct ttgtctgtaa agctatccat g
//
LOCUS       DACDXO010000232         2371 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E23605_2017
            SAMN12639989-rid9881463.guided.149, whole genome shotgun sequence.
ACCESSION   DACDXO010000232 DACDXO010000000
VERSION     DACDXO010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639989
            Sequence Read Archive: SRR10023389
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741914.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:42:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,972
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 33 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 17 of 73
            Pseudo Genes (multiple problems)  :: 10 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2371
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639989-rid9881463.guided.149"
                     /isolate="E23605_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            22..1941
                     /gene="tet(M)"
                     /locus_tag="IWM35_002960"
     CDS             22..1941
                     /gene="tet(M)"
                     /locus_tag="IWM35_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019117172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HAP9920715.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYTSGKLLEALELEQEESIRFHNCSLFPVYH
                     GSAKNNIGIDNLIEVITNKFYSSTHRGQSELCGKVFKIEYSEKRQRLAYIRLYSGVLH
                     LRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRKKIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIEIKEPTVIYMERPLKKAEYTIHIEVPPNPFWASIGLSVA
                     QLPLGSGVQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            2060..2227
                     /locus_tag="IWM35_002961"
     CDS             2060..2227
                     /locus_tag="IWM35_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP9920716.1"
                     /translation="MCPVCGNKTRLKIREDTELKKFPLYCPKCRQENLIEIKQFKVTV
                     ITEPDAKTQSR"
     gene            complement(2287..>2371)
                     /locus_tag="IWM35_002962"
     CDS             complement(2287..>2371)
                     /locus_tag="IWM35_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAP9920717.1"
                     /translation="MDSFTDKELSLMESLASGINEARNIED"
BASE COUNT          817 a          361 c          483 g          710 t
ORIGIN      
        1 ttttgaatgg aggaaaatca catgaaaatt attaatattg gagttttagc tcatgttgat
       61 gcgggaaaaa ctaccttaac agaaagctta ttatataaca gtggagcgat tacagaatta
      121 ggaagcgtgg acaaaggtac aacgaggacg gataatacgc ttttagaacg tcagagagga
      181 attacaattc agacaggaat aacctctttt cagtgggaaa atacgaaggt gaacatcata
      241 gacacgccag gacatatgga tttcttagca gaagtatatc gttcattatc agttttagat
      301 ggggcaattc tactgatttc tgcaaaagat ggcgtacaag cacaaactcg tatattattt
      361 catgcactta ggaaaatggg gattcccaca atctttttta tcaataagat tgaccaaaat
      421 ggaattgatt tatcaacggt ttatcaggat attaaagaga aactttctgc cgaaattgta
      481 atcaaacaga aggtagaact gtatcctaat atgtgtgtga cgaactttac cgaatctgaa
      541 caatgggata cggtaataga gggaaacgat gaccttttag agaaatatac gtctgggaaa
      601 ttattggaag cattagaact cgaacaagag gaaagcataa gatttcataa ttgttccctg
      661 ttccctgttt atcacggaag tgcaaaaaac aatataggga ttgataacct tatagaagtg
      721 attacgaata aattttattc atcaacacat cgaggtcagt ctgaactttg cggaaaagtt
      781 ttcaaaattg agtattcgga aaaaagacag cgtcttgcat atatacgtct ttatagtggc
      841 gtactgcatt tgcgagattc ggttagaata tcggaaaagg aaaaaataaa aattacagaa
      901 atgtatactt caataaatgg tgaattatgt aaaatcgata aggcttattc cggggaaatt
      961 gttattttgc aaaatgagtt tttgaagtta aatagtgttc ttggagatac aaaactattg
     1021 ccacagagaa aaaagattga aaatccgcac cctctactac aaacaactgt tgaaccgagt
     1081 aaacctgaac agagagaaat gttgcttgat gcccttttgg aaatctcaga tagtgatccg
     1141 cttctacgat attacgtgga ttctacgaca catgaaatta tactttcttt cttagggaaa
     1201 gtacaaatgg aagtgattag tgcactgttg caagaaaagt atcatgtgga gatagaaata
     1261 aaagagccta cagtcattta tatggaaaga ccgttaaaaa aagcagagta taccattcac
     1321 atcgaagttc caccgaatcc tttctgggct tccattggtc tatctgtagc acagcttcca
     1381 ttagggagcg gagtacagta tgagagctcg gtttctcttg gatacttaaa tcaatcgttt
     1441 caaaatgcag ttatggaggg gatacgctat ggctgtgaac aaggattgta tggttggaat
     1501 gtgacggact gtaaaatctg ttttaagtat ggcttatact atagccctgt tagtacccca
     1561 gcagattttc ggatgcttgc tcctattgta ttggaacaag tcttaaaaaa agctggaaca
     1621 gaattgttag agccatatct tagttttaaa atttatgcgc cacaggaata tctttcacga
     1681 gcatacaacg atgctcctaa atattgtgcg aacatcgtag acactcaatt gaaaaataat
     1741 gaggtcattc ttagtggaga aatccctgct cggtgtattc aagaatatcg tagtgattta
     1801 actttcttta caaatggacg tagtgtttgt ttaacagagt taaaagggta ccatgttact
     1861 accggtgaac ctgtttgcca gccccgtcgt ccaaatagtc ggatagataa agtacgatat
     1921 atgttcaata aaataactta gtgtatttta tgttgttata taaatatggt ttcttgttaa
     1981 ataagatgaa atatttttta ataaagattt gaattaaagt gtaaaggagg agatagttat
     2041 tataaactac aagtggatat tgtgtcctgt atgtggaaat aaaacacgat taaagataag
     2101 ggaagatact gaattaaaaa aattccccct ctattgtccg aaatgcagac aagaaaattt
     2161 aattgaaata aagcagttca aagtaactgt gattacagag ccagacgcaa agacgcagag
     2221 ccgataaaat gagattaata caatctcatt ttatcggctc tttccgttat gtatggattc
     2281 ttttaattag tcttcgatgt ttcttgcttc gttgataccg ctggctaaag attccattaa
     2341 ggatagttct ttgtctgtaa agctatccat g
//
LOCUS       DACDXO010000233         1504 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E23605_2017
            SAMN12639989-rid9881463.guided.160, whole genome shotgun sequence.
ACCESSION   DACDXO010000233 DACDXO010000000
VERSION     DACDXO010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639989
            Sequence Read Archive: SRR10023389
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741914.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:42:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,972
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 33 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 17 of 73
            Pseudo Genes (multiple problems)  :: 10 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1504
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639989-rid9881463.guided.160"
                     /isolate="E23605_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            262..373
                     /locus_tag="IWM35_002963"
                     /pseudo
     CDS             262..373
                     /locus_tag="IWM35_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008787626.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
     gene            394..477
                     /locus_tag="IWM35_002964"
     CDS             394..477
                     /locus_tag="IWM35_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuator leader
                     peptide ErmL"
                     /protein_id="HAP9920718.1"
                     /translation="MLVFQMRYVDKTSTVLKQTKNSDYADK"
     gene            602..1339
                     /gene="erm(B)"
                     /locus_tag="IWM35_002965"
     CDS             602..1339
                     /gene="erm(B)"
                     /locus_tag="IWM35_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HAP9920719.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
BASE COUNT          551 a          224 c          245 g          484 t
ORIGIN      
        1 atttgtatcg ataagaaata gatttaaaaa tttcgctgtt attttgtaca tttaacttga
       61 cggtgacatc tctctattgt gagttattag tggtacagtt ttcaaccgtt ttaattataa
      121 aaaagtggtg catttttaaa ttggcacaaa caggtaacgg ttattgcagg tgtatttctt
      181 atctatgggt ttaacatgga ttttatcatt aaaatcatga gtattgtccg agagtgattg
      241 gtcttgcgta tggttaaccc taaagttatg gaaataagac ttagaagcaa acttaagagt
      301 gtgttgatag tgcattatct taaaattttg tataatagga attgaagtta aattagatgc
      361 taaaaatttg taattaagaa ggagggattc gtcatgttgg tattccaaat gcgttatgta
      421 gataaaacat ctactgtttt gaaacagact aaaaacagtg attacgcaga taaataaata
      481 cgttagatta attcctacca gtgactaatc ttatgacttt ttaaacagat aactaaaatt
      541 acaaacaaat cgtttaactt ctgtatttgt ttatagatgt aatcacttca ggagtgataa
      601 catgaacaaa aatataaaat attctcaaaa ctttttaacg agtgaaaaag tactcaacca
      661 aataataaaa caattgaatt taaaagaaac cgataccgtt tacgaaattg gaacaggtaa
      721 agggcattta acgacgaaac tggctaaaat aagtaaacag gtaacgtcta ttgaattaga
      781 cagtcatcta ttcaacttat cgtcagaaaa attaaaactg aatactcgtg tcactttaat
      841 tcaccaagat attctacagt ttcaattccc taacaaacag aggtataaaa ttgttgggaa
      901 tattccttac catttaagca cacaaattat taaaaaagtg gtttttgaaa gccgtgcgtc
      961 tgacatctat ctgattgttg aagaaggatt ctacaagcgt accttggata ttcaccgaac
     1021 actagggttg ctcttgcaca ctcaagtctc gattcagcaa ttgcttaagc tgccagcgga
     1081 atgctttcat cctaaaccaa aagtaaacag tgtcttaata aaacttaccc gccataccac
     1141 agatgttcca gataaatatt ggaagctata tacgtacttt gtttcaaaat gggtcaatcg
     1201 agaatatcgt caactgttta ctaaaaatca gtttcatcaa gcaatgaaac acgccaaagt
     1261 aaacaattta agtaccgtta cttatgagca agtattgtct atttttaata gttatctatt
     1321 atttaacggg aggaaataat tctatgagtc gcttttgtaa atttggaaag ttacacgtta
     1381 ctaaagggaa tgtagataaa ttattaggta tagggcacct ctaataacta ccaattaatt
     1441 cacctcaaaa tgaaattaga aaaaaacagc gaaattttta aatctatttc ttatcgatac
     1501 aaat
//
LOCUS       DACDXO010000234         1477 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E23605_2017
            SAMN12639989-rid9881463.guided.161, whole genome shotgun sequence.
ACCESSION   DACDXO010000234 DACDXO010000000
VERSION     DACDXO010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639989
            Sequence Read Archive: SRR10023389
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1477)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741914.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:42:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,972
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 33 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 17 of 73
            Pseudo Genes (multiple problems)  :: 10 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1477
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639989-rid9881463.guided.161"
                     /isolate="E23605_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            9..1049
                     /locus_tag="IWM35_002966"
     CDS             9..1049
                     /locus_tag="IWM35_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HAP9920720.1"
                     /translation="MYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLPQRK
                     KIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLGKVQ
                     MEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVSPLP
                     LGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYSPVS
                     TPADFRMLTPIVLEQAFRKAGTELLEPYLSFKVYAPQEYLSRAYNDAPKYCANIVNTQ
                     LKNNEVIIIGEIPARCIQDYRNDLTFFTNGLSVCLAELKGYQVTTGEPVCQTRRLNSR
                     IDKVRYMFNKIT"
     gene            1147..1335
                     /locus_tag="IWM35_002967"
     CDS             1147..1335
                     /locus_tag="IWM35_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP9920721.1"
                     /translation="MINYKWILCPICGNKTRLRIRVDTILKNFPLYCPKCKNETLINV
                     QKMNIITIKEPDAKTQSR"
     gene            complement(1393..>1477)
                     /locus_tag="IWM35_002968"
     CDS             complement(1393..>1477)
                     /locus_tag="IWM35_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAP9920722.1"
                     /translation="MDSFTDKELSLMEALAKGINEARNIEG"
BASE COUNT          489 a          231 c          282 g          475 t
ORIGIN      
        1 ttacagaaat gtatacttca ataaatggtg aattatgtaa gattgataga gcttattctg
       61 gagaaattgt tattttgcaa aatgagtttt tgaagttaaa tagtgttctt ggagatacaa
      121 aactattgcc acagagaaaa aagattgaaa atccgcaccc tctactacaa acaactgttg
      181 aaccgagtaa acctgaacag agagaaatgt tgcttgatgc ccttttggaa atctcagata
      241 gtgatccgct tctacgatat tacgtggatt ctacgacaca tgaaattata ctttctttct
      301 tagggaaagt acaaatggaa gtgattagtg cactgttgca agaaaagtat catgtggaga
      361 tagaactaaa agagcctaca gtcatttata tggagagacc gttaaaaaat gcagaatata
      421 ccattcacat cgaagtgccg ccaaatcctt tctgggcttc cattggttta tctgtatcac
      481 cgcttccgtt gggaagtgga atgcagtatg agagctcggt ttctcttgga tacttaaatc
      541 aatcatttca aaatgcagtt atggaaggga tacgctatgg ttgcgaacaa ggattatatg
      601 gttggaatgt gacggattgt aaaatctgtt ttaagtacgg tttatactat agccctgtta
      661 gtactccagc agattttcgg atgcttactc ctattgtact ggagcaagcc tttagaaaag
      721 ctggaacaga attgttagag ccatatctta gttttaaagt ttatgcacca caggaatatc
      781 tttcacgggc atataacgat gctcccaaat attgtgcaaa tatcgtaaat actcaactga
      841 aaaataatga ggtcattatt attggagaaa ttcctgctcg atgtattcaa gattatcgca
      901 atgatttaac tttttttaca aatgggctta gtgtttgttt agcagagcta aaaggatatc
      961 aggttaccac tggcgaacct gtttgccaga cccgtcgtct aaatagtcgg atagataaag
     1021 taagatatat gttcaataaa ataacttagt gcgttttatg ttgttatata aatatggttt
     1081 cttattaaat aagatgaaat attctttaat atagatttga attaaagtgg aaaggaggag
     1141 attgttatta taaactacaa gtggatattg tgtcctattt gtggaaataa aacaagacta
     1201 cgaatacgag tggatactat acttaaaaat ttccctttat actgccccaa atgtaagaac
     1261 gaaactttaa ttaatgttca aaaaatgaat ataataacaa tcaaagagcc agacgccaag
     1321 acgcagagcc gataatttga gaaatgaaac tctcatctta tcggctcttt ttgtttatct
     1381 gaattttact gactagcctt caatatttct tgcttcattg ataccttttg ctagtgcttc
     1441 cattaaggat agttctttgt ctgtaaagct atccatg
//
LOCUS       DACDXO010000235         1422 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E23605_2017
            SAMN12639989-rid9881463.guided.164, whole genome shotgun sequence.
ACCESSION   DACDXO010000235 DACDXO010000000
VERSION     DACDXO010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639989
            Sequence Read Archive: SRR10023389
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741914.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:42:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,972
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 33 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 17 of 73
            Pseudo Genes (multiple problems)  :: 10 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1422
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639989-rid9881463.guided.164"
                     /isolate="E23605_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..1351
                     /locus_tag="IWM35_002969"
     CDS             <1..1351
                     /locus_tag="IWM35_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional aminoglycoside N-acetyltransferase
                     AAC(6')-Ie/aminoglycoside O-phosphotransferase
                     APH(2'')-Ia"
                     /protein_id="HAP9920723.1"
                     /translation="RDFILYLKLCNRTESLKKHYTEPWEDEVFRVIIEYNNVPIGYGQ
                     IYKMYDELYTDYHYPKTDEIVYGMDQFIGEPNYWSKGIGTRYIKLIFEFLKKERNANA
                     VILDPHKNNPRAIRAYQKSGFRIIEDLPEHELHEGKKEDCYLMEYRYDDNATNVKAMK
                     YLIEHYFDNFKVDSIEIIGSGYDSVAYLVNNEYIFKTKFSTNKKKGYAKEKAIYNFLN
                     TNLETNVKIPNIEYSYISDELSILGYKEIKGTFLTPEIYSTMSEEEQNLLKRDIASFL
                     RQMHGLDYTDISECTIDNKQNVLEEYILLRETIYNDLTDIEKDYIESFMERLNATTVF
                     EGKKCLCHNDFSCNHLLLDGNNRLTGIIDFGDSGIIDEYCDFIYLLEDSEEEIGTNFG
                     EDILRMYGNIDIEKAKEYQDIVEEYYPIETIVYGIKNIKQEFIENGRKEIYKRTYKD"
BASE COUNT          598 a          114 c          239 g          471 t
ORIGIN      
        1 aagggatttt attctttatt taaaactttg caacagaacc gaatcattaa aaaaacatta
       61 tacagagcct tgggaagatg aagtttttag agtaattatt gaatataaca atgttcctat
      121 tggatatgga caaatatata aaatgtatga tgagttatat actgattatc attatccaaa
      181 aactgatgag atagtctatg gtatggatca atttatagga gagccaaatt attggagtaa
      241 aggaattggt acaagatata ttaaattgat ttttgaattt ttgaaaaaag aaagaaatgc
      301 taatgcagtt attttagacc ctcataaaaa taatccaaga gcaataaggg cataccaaaa
      361 atctggtttt agaattattg aagatttgcc agaacatgaa ttacacgagg gcaaaaaaga
      421 agattgttat ttaatggaat atagatatga tgataatgcc acaaatgtta aggcaatgaa
      481 atatttaatt gagcattact ttgataattt caaagtagat agtattgaaa taatcggtag
      541 tggttatgat agtgtggcat atttagttaa taatgaatac atttttaaaa caaaatttag
      601 tactaataag aaaaaaggtt atgcaaaaga aaaagcaata tataattttt taaatacaaa
      661 tttagaaact aatgtaaaaa ttcctaatat tgaatattcg tatattagtg atgaattatc
      721 tatactaggt tataaagaaa ttaaaggaac ttttttaaca ccagaaattt attctactat
      781 gtcagaagaa gaacaaaatt tgttaaaacg agatattgcc agttttttaa gacaaatgca
      841 cggtttagat tatacagata ttagtgaatg tactattgat aataaacaaa atgtattaga
      901 agagtatata ttgttgcgtg aaactattta taatgattta actgatatag aaaaagatta
      961 tatagaaagt tttatggaaa gactaaatgc aacaacagtt tttgagggta aaaagtgttt
     1021 atgccataat gattttagtt gtaatcatct attgttagat ggcaataata gattaactgg
     1081 aataattgat tttggagatt ctggaattat agatgaatat tgtgatttta tatacttact
     1141 tgaagatagt gaagaagaaa taggaacaaa ttttggagaa gatatattaa gaatgtatgg
     1201 aaatatagat attgagaaag caaaagaata tcaagatata gttgaagaat attatcctat
     1261 tgaaactatt gtttatggaa ttaaaaatat taaacaggaa tttatcgaaa atggtagaaa
     1321 agaaatttat aaaaggactt ataaagattg attatataat atatgaaaag ctattataaa
     1381 agacggttct gttgcaaagt tttaaatcta ctatcaaata ag
//
LOCUS       DACDXO010000236         1350 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E23605_2017
            SAMN12639989-rid9881463.guided.167, whole genome shotgun sequence.
ACCESSION   DACDXO010000236 DACDXO010000000
VERSION     DACDXO010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639989
            Sequence Read Archive: SRR10023389
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741914.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:42:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,972
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 33 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 17 of 73
            Pseudo Genes (multiple problems)  :: 10 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1350
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639989-rid9881463.guided.167"
                     /isolate="E23605_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            47..298
                     /locus_tag="IWM35_002970"
                     /pseudo
     CDS             47..298
                     /locus_tag="IWM35_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            391..1185
                     /locus_tag="IWM35_002971"
                     /note="aph(3')-IIIa"
     CDS             391..1185
                     /locus_tag="IWM35_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HAP9920724.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
BASE COUNT          425 a          234 c          322 g          369 t
ORIGIN      
        1 acattttcct ctaggaaaaa attaactagc accacgggtt atcttttata tagaagatat
       61 cgccgtatgt aaggatttca gggggcaagg cataggcagc gcgcttatca atatatctat
      121 agaatgggca aagcataaaa acttgcatgg actaatgctt gaaacccagg acaataacct
      181 tatagcttgt aaattctatc ataattgtgg tttcaaaatc ggctccgtcg atactatgtt
      241 atacgccaac tttgaaaaca actttgaaaa agctgttttc tggtatttaa ggttttagaa
      301 tgcaaggaac agtgaattgg agttcgtctt gttataatta gcttcttggg gtatctttaa
      361 atactgtaga aaagaggaag gaaataataa atggctaaaa tgagaatatc accggaattg
      421 aaaaaactga tcgaaaaata ccgctgcgta aaagatacgg aaggaatgtc tcctgctaag
      481 gtatataagc tggtgggaga aaatgaaaac ctatatttaa aaatgacgga cagccggtat
      541 aaagggacca cctatgatgt ggaacgggaa aaggacatga tgctatggct ggaaggaaag
      601 ctgcctgttc caaaggtcct gcactttgaa cggcatgatg gctggagcaa tctgctcatg
      661 agtgaggccg atggcgtcct ttgctcggaa gagtatgaag atgaacaaag ccctgaaaag
      721 attatcgagc tgtatgcgga gtgcatcagg ctctttcact ccatcgacat atcggattgt
      781 ccctatacga atagcttaga cagccgctta gccgaattgg attacttact gaataacgat
      841 ctggccgatg tggattgcga aaactgggaa gaagacactc catttaaaga tccgcgcgag
      901 ctgtatgatt ttttaaagac ggaaaagccc gaagaggaac ttgtcttttc ccacggcgac
      961 ctgggagaca gcaacatctt tgtgaaagat ggcaaagtaa gtggctttat tgatcttggg
     1021 agaagcggca gggcggacaa gtggtatgac attgccttct gcgtccggtc gatcagggag
     1081 gatatcgggg aagaacagta tgtcgagcta ttttttgact tactggggat caagcctgat
     1141 tgggagaaaa taaaatatta tattttactg gatgaattgt tttagtacct agatttagat
     1201 gtctaaaaag ctttaactac aagcttttta gacatctaat cttttctgaa gtacatccgc
     1261 aactgtccat actctgatgt tttatatctt ttctaaaagt tcgctagata ggggtcccga
     1321 gcgcctacga ggaatttgta tcgataagaa
//
LOCUS       DACDXO010000237         1194 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E23605_2017
            SAMN12639989-rid9881463.guided.179, whole genome shotgun sequence.
ACCESSION   DACDXO010000237 DACDXO010000000
VERSION     DACDXO010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639989
            Sequence Read Archive: SRR10023389
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741914.1
            Genome Coverage       :: 79x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:42:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,972
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,836
            CDSs (with protein)               :: 2,836
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 33 of 73
            Pseudo Genes (incomplete)         :: 35 of 73
            Pseudo Genes (internal stop)      :: 17 of 73
            Pseudo Genes (multiple problems)  :: 10 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1194
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639989-rid9881463.guided.179"
                     /isolate="E23605_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            259..>1194
                     /gene="vanY"
                     /locus_tag="IWM35_002972"
     CDS             259..>1194
                     /gene="vanY"
                     /locus_tag="IWM35_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanY-A/VanY-F/VanY-M family D-Ala-D-Ala
                     carboxypeptidase"
                     /protein_id="HAP9920725.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNWFCCKVLNLLSN"
BASE COUNT          426 a          173 c          238 g          357 t
ORIGIN      
        1 cttatttgat agtagattta aaactttgca acagaacccc cattgtcaac aggcagttca
       61 gcctcgttaa attcagcatg ggtatcactt atgaaaattc atctacattg gtgataatag
      121 taaatccagt agggcgaaat aattgactgt aatttacggg gcaaaacggc acaatctcaa
      181 acgagattgt gccgtttaag gggaagattc tagaaatatt tcatacttcc aactatatag
      241 ttaaggagga gactgaaaat gaagaagttg ttttttttat tgttattgtt attcttaata
      301 tacttaggtt atgactacgt taatgaagca ctgttttctc aggaaaaagt cgaatttcaa
      361 aattatgatc aaaatcccaa agaacattta gaaaatagtg ggacttctga aaatacccaa
      421 gagaaaacaa ttacagaaga acaggtttat caaggaaatc tgctattaat caatagtaaa
      481 tatcctgttc gccaagaaag tgtgaagtca gatatcgtga atttatctaa acatgacgaa
      541 ttaataaatg gatacgggtt gcttgatagt aatatttata tgtcaaaaga aatagcacaa
      601 aaattttcag agatggtcaa tgatgctgta aagggtggcg ttagtcattt tattattaat
      661 agtggctatc gagactttga tgagcaaagt gtgctttacc aagaaatggg ggctgagtat
      721 gccttaccag caggttatag tgagcataat tcaggtttat cactagatgt aggatcaagc
      781 ttgacgaaaa tggaacgagc ccctgaagga aagtggatag aagaaaatgc ttggaaatac
      841 gggttcattt tacgttatcc agaggacaaa acagagttaa caggaattca atatgaacca
      901 tggcatattc gctatgttgg tttaccacat agtgcgatta tgaaagaaaa gaatttcgtt
      961 ctcgaggaat atatggatta cctaaaagaa gaaaaaacca tttctgttag tgtaaatggg
     1021 gaaaaatatg agatctttta ttatcctgtt actaaaaata ccaccattca tgtgccgact
     1081 aatcttcgtt atgagatatc aggaaacaat atagacggtg taattgtgac agtgtttccc
     1141 ggatcaacac atactaattg gttctgttgc aaagttttaa atctactatc aaat
//
